LOCUS       DAXHRI010000001        47815 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXHRI010000001 DAXHRI010000000
VERSION     DAXHRI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 47815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.001"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..261
                     /locus_tag="ACFM75_000001"
     CDS             <1..261
                     /locus_tag="ACFM75_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254626.1"
                     /translation="LTACSSTSAPSATEIKVVEKAVLPTPPAALMVAPVRPNPPKDGK
                     TATLLEHAAEFGGYVAELENQNQAWRDWVNSQAEVDGSEGAR"
     gene            258..587
                     /locus_tag="ACFM75_000002"
     CDS             258..587
                     /locus_tag="ACFM75_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254627.1"
                     /translation="MTTYRDLVQRTVACRHADLELGLSRAREQEPFVIHVSELLDKAG
                     IEYAVRMDKDFQTTFCVEFSATAPADVIGILQKYYSVFFDGQKVEAASRNPEGYAVRI
                     VFGDVPV"
     gene            599..865
                     /locus_tag="ACFM75_000003"
     CDS             599..865
                     /locus_tag="ACFM75_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254628.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISSRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAQDDRKNIAASLERIENKLEKVNEKLDRKADKS"
     gene            862..1059
                     /locus_tag="ACFM75_000004"
     CDS             862..1059
                     /locus_tag="ACFM75_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254629.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            1062..1566
                     /locus_tag="ACFM75_000005"
                     /pseudo
     CDS             1062..1566
                     /locus_tag="ACFM75_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
     gene            complement(1563..2075)
                     /locus_tag="ACFM75_000006"
     CDS             complement(1563..2075)
                     /locus_tag="ACFM75_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254630.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLTDGY"
     gene            2087..3709
                     /gene="terL"
                     /locus_tag="ACFM75_000007"
     CDS             2087..3709
                     /gene="terL"
                     /locus_tag="ACFM75_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC6254631.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRNKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            3709..5277
                     /locus_tag="ACFM75_000008"
     CDS             3709..5277
                     /locus_tag="ACFM75_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFC6254632.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     NGTTATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            5264..6559
                     /locus_tag="ACFM75_000009"
     CDS             5264..6559
                     /locus_tag="ACFM75_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFC6254633.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            6669..7085
                     /locus_tag="ACFM75_000010"
     CDS             6669..7085
                     /locus_tag="ACFM75_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFC6254634.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            7316..8377
                     /locus_tag="ACFM75_000011"
     CDS             7316..8377
                     /locus_tag="ACFM75_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFC6254635.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            8439..8948
                     /locus_tag="ACFM75_000012"
                     /pseudo
     CDS             8439..8948
                     /locus_tag="ACFM75_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8938..10083
                     /locus_tag="ACFM75_000013"
     CDS             8938..10083
                     /locus_tag="ACFM75_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFC6254636.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGGRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKVENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            10080..10748
                     /locus_tag="ACFM75_000014"
     CDS             10080..10748
                     /locus_tag="ACFM75_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFC6254637.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            10849..11196
                     /locus_tag="ACFM75_000015"
     CDS             10849..11196
                     /locus_tag="ACFM75_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFC6254638.1"
                     /translation="MDKELNPSIGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            11210..12264
                     /locus_tag="ACFM75_000016"
                     /pseudo
     CDS             11210..12264
                     /locus_tag="ACFM75_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            12261..12821
                     /locus_tag="ACFM75_000017"
     CDS             12261..12821
                     /locus_tag="ACFM75_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFC6254639.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            12832..14808
                     /locus_tag="ACFM75_000018"
     CDS             12832..14808
                     /locus_tag="ACFM75_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HFC6254640.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAANGLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSALVSTWTDENLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETVNAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGSPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSLIVPEI
                     PETNEEPQRESGRTSTGPRNRRRHRDGLLEALQD"
     gene            complement(15690..15992)
                     /locus_tag="ACFM75_000019"
     CDS             complement(15690..15992)
                     /locus_tag="ACFM75_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFC6254641.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(15964..16251)
                     /locus_tag="ACFM75_000020"
     CDS             complement(15964..16251)
                     /locus_tag="ACFM75_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFC6254642.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            16331..16936
                     /locus_tag="ACFM75_000021"
     CDS             16331..16936
                     /locus_tag="ACFM75_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254643.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            16915..17211
                     /locus_tag="ACFM75_000022"
     CDS             16915..17211
                     /locus_tag="ACFM75_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC6254644.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            17208..17417
                     /locus_tag="ACFM75_000023"
     CDS             17208..17417
                     /locus_tag="ACFM75_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254645.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(17587..18670)
                     /locus_tag="ACFM75_000024"
                     /pseudo
     CDS             complement(17587..18670)
                     /locus_tag="ACFM75_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
     gene            complement(18959..19606)
                     /locus_tag="ACFM75_000025"
     CDS             complement(18959..19606)
                     /locus_tag="ACFM75_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC6254646.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(19603..19971)
                     /locus_tag="ACFM75_000026"
     CDS             complement(19603..19971)
                     /locus_tag="ACFM75_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC6254647.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(19981..20652)
                     /locus_tag="ACFM75_000027"
     CDS             complement(19981..20652)
                     /locus_tag="ACFM75_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC6254648.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIIYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(20817..21536)
                     /locus_tag="ACFM75_000028"
     CDS             complement(20817..21536)
                     /locus_tag="ACFM75_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6254649.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(21620..22933)
                     /gene="hpnE"
                     /locus_tag="ACFM75_000029"
     CDS             complement(21620..22933)
                     /gene="hpnE"
                     /locus_tag="ACFM75_000029"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC6254650.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTIPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(23012..23884)
                     /gene="hpnD"
                     /locus_tag="ACFM75_000030"
     CDS             complement(23012..23884)
                     /gene="hpnD"
                     /locus_tag="ACFM75_000030"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC6254651.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            24055..25917
                     /gene="hscA"
                     /locus_tag="ACFM75_000031"
     CDS             24055..25917
                     /gene="hscA"
                     /locus_tag="ACFM75_000031"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC6254652.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCHLLEQNELSKLNEQDSRLLLSLVRSAKEK
                     LTAQTEAVIETTLSDGHKVHTVITRQEFHNLTQNLVQKTIEPVKQALKDAGVGKADVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTSRAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQVDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDSTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELAQI
                     QRDIADLQGSLKDGNAEDIRAAAAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            25999..26655
                     /locus_tag="ACFM75_000032"
     CDS             25999..26655
                     /locus_tag="ACFM75_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6254653.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLEFFKSESDAEGRKPR
                     "
     gene            26691..27452
                     /locus_tag="ACFM75_000033"
     CDS             26691..27452
                     /locus_tag="ACFM75_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC6254654.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNIEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            27507..27848
                     /gene="fdx"
                     /locus_tag="ACFM75_000034"
     CDS             27507..27848
                     /gene="fdx"
                     /locus_tag="ACFM75_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC6254655.1"
                     /translation="MPKITVLPHTTLCPEGAVIDDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(28031..28618)
                     /locus_tag="ACFM75_000035"
     CDS             complement(28031..28618)
                     /locus_tag="ACFM75_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC6254656.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDPLSEMGEFDRLILLIRKLYQILD
                     GQHTLSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSDGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGRFVRK
                     PIKINIEARVERYPAYCPTVALKVTRRRFFRLQNR"
     gene            28831..29028
                     /gene="iscX"
                     /locus_tag="ACFM75_000036"
     CDS             28831..29028
                     /gene="iscX"
                     /locus_tag="ACFM75_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC6254657.1"
                     /translation="MKWTDTQRIAEDLYDLHGEDIDPKSLRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(29096..29497)
                     /locus_tag="ACFM75_000037"
     CDS             complement(29096..29497)
                     /locus_tag="ACFM75_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6254658.1"
                     /translation="MKDKHHSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDTKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            29708..30667
                     /locus_tag="ACFM75_000038"
     CDS             29708..30667
                     /locus_tag="ACFM75_000038"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC6254659.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            30735..32045
                     /gene="tilS"
                     /locus_tag="ACFM75_000039"
     CDS             30735..32045
                     /gene="tilS"
                     /locus_tag="ACFM75_000039"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC6254660.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAASDTLAVGGIHKNVKKILQEKRVLPT
                     LRPIWPLVADSGNSPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            32119..32904
                     /locus_tag="ACFM75_000040"
     CDS             32119..32904
                     /locus_tag="ACFM75_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC6254661.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGKPLGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLSCADDILALIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLDRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLARYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            32914..33111
                     /locus_tag="ACFM75_000041"
     CDS             32914..33111
                     /locus_tag="ACFM75_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254662.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDEQWK
                     LILLSNAVAAAVLAWVWWKWF"
     gene            33124..33426
                     /locus_tag="ACFM75_000042"
     CDS             33124..33426
                     /locus_tag="ACFM75_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC6254663.1"
                     /translation="MKTSKIIAGLMLAVAVQTASAAVYECRQNGKVSYSQTPGKHCTN
                     AGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            33488..34864
                     /gene="mpl"
                     /locus_tag="ACFM75_000043"
     CDS             33488..34864
                     /gene="mpl"
                     /locus_tag="ACFM75_000043"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC6254664.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIDVYEGFDAAQLDEFKADVYVIGNVAKRGMDVVEAILNLGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTIASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            35071..36123
                     /gene="bioB"
                     /locus_tag="ACFM75_000044"
     CDS             35071..36123
                     /gene="bioB"
                     /locus_tag="ACFM75_000044"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC6254665.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(36270..36836)
                     /locus_tag="ACFM75_000045"
     CDS             complement(36270..36836)
                     /locus_tag="ACFM75_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6254666.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKPLSGGLFI"
     gene            complement(36960..37199)
                     /locus_tag="ACFM75_000046"
     CDS             complement(36960..37199)
                     /locus_tag="ACFM75_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254667.1"
                     /translation="MGGCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            37186..37419
                     /locus_tag="ACFM75_000047"
     CDS             37186..37419
                     /locus_tag="ACFM75_000047"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6254668.1"
                     /translation="MQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGIT
                     ATAAGGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(37482..39341)
                     /gene="ilvD"
                     /locus_tag="ACFM75_000048"
     CDS             complement(37482..39341)
                     /gene="ilvD"
                     /locus_tag="ACFM75_000048"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC6254669.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDITNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(39514..41283)
                     /gene="cysI"
                     /locus_tag="ACFM75_000049"
     CDS             complement(39514..41283)
                     /gene="cysI"
                     /locus_tag="ACFM75_000049"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC6254670.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILSELDDLIGKWAAGRDTDEGFGDFAIRTGI
                     VKPVLDAPVDFWDASKAVPIARA"
     gene            complement(41310..43160)
                     /locus_tag="ACFM75_000050"
     CDS             complement(41310..43160)
                     /locus_tag="ACFM75_000050"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC6254671.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGNGASAGNGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEA
                     AGIQVSRTELKDYKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKL
                     QFAVLGLGDSSYPNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASADGWTGRIVAR
                     LKEEAAKNRATPAPQTTPPAGLQTAPDGRYCKADPFPAALLANQKITSRQSDKDVRHI
                     EIDLSGSDLHYLPGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLS
                     HFELTQNTPAFVKGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQ
                     FAGLLRPLAPRLYSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDG
                     TVRVFVERNDGFRLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPH
                     FARDFLYQTEWQQFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHI
                     YVCGDAAKMAKDVEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(43157..44443)
                     /locus_tag="ACFM75_000051"
     CDS             complement(43157..44443)
                     /locus_tag="ACFM75_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC6254672.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAGRLGLKDVHFVPVSALLGDNLVYPGGNMPWYRGEPLLAIL
                     ENLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARRYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(44482..45405)
                     /gene="cysD"
                     /locus_tag="ACFM75_000052"
     CDS             complement(44482..45405)
                     /gene="cysD"
                     /locus_tag="ACFM75_000052"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFC6254673.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPAEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(45443..46183)
                     /locus_tag="ACFM75_000053"
     CDS             complement(45443..46183)
                     /locus_tag="ACFM75_000053"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC6254674.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(46194..47645)
                     /gene="cysG"
                     /locus_tag="ACFM75_000054"
     CDS             complement(46194..47645)
                     /gene="cysG"
                     /locus_tag="ACFM75_000054"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC6254675.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQAYPALGGLNAGQRAA"
BASE COUNT        10887 a        12827 c        13642 g        10459 t
ORIGIN      
        1 ctcacggctt gcagctctac aagcgcgccc tcggctacgg aaattaaggt tgtcgaaaag
       61 gcggtcttgc cgacaccgcc tgccgcattg atggttgcgc cggtacgccc aaatccgccg
      121 aaagacggca agacggccac gctgttggaa cacgccgccg agtttggcgg ctatgtggcg
      181 gagttggaaa accaaaatca ggcttggcgc gactgggtca acagtcaagc ggaagttgac
      241 ggttcggagg gcgcgcgatg acgacttatc gtgatttggt gcaacgcacg gtcgcctgcc
      301 gccatgcgga cttggagtta ggcttaagcc gcgcacgcga acaagagccg tttgtcattc
      361 atgtttccga actgttggat aaggcaggca ttgagtacgc agtgcgtatg gataaggatt
      421 ttcagacgac cttttgtgtg gagttttccg cgaccgcccc tgctgatgtg attggtattt
      481 tgcaaaagta ttattcagtc ttttttgacg gccaaaaggt cgaggcggcg agccgaaatc
      541 ccgaaggcta tgcggtgcgt atcgtattcg gcgatgtgcc ggtttaaagg ggttttaaat
      601 ggactttgaa tttggtttca gaaccctgtg gccgattgcg acggcggcat tttggttttg
      661 ggtcaacggc atttcaagcc gtctgaaaga ggcggacaag cgtatcgacg accttaaaga
      721 ggagctgcac gcggtcaagc tctcgtacca caccaagcag gatgcccaag acgaccgcaa
      781 aaatatcgca gcgtctttgg agcgcatcga aaacaagttg gaaaaagtaa acgaaaaact
      841 ggacaggaaa gcggacaaat catgagcgac ccgattttgg aagccttggc gcgtattgaa
      901 aacaagactg atcaaacgct gaaaaatcag aaggaaatgc aggcggaaat tgcgcaaatc
      961 cgccaagaca cgaaacgcac ggccattaca ttcggcgcac tgggcggcgg cgtgattacg
     1021 gtcggctggg aattgcttaa agcgaaaatg ggactgtaat tatggctcac ccgcaagaaa
     1081 tccgtgaaaa gttacgccgg ctctatgtca gcggcgagca aactttggaa acggcggcct
     1141 tgatgtgcga aatcccgcag gccactgcgc gtgcgtggaa acgtgcggat aaggaaaaag
     1201 gcgacgactg ggataagatg cgcgccgctt acactttggc cggtggcggt attgaggatt
     1261 tgagccgtgc gatgttggcc ggttttatgg tgcagtacaa cagcacgatg acgatgctgc
     1321 aggattcgag taccgaagat ttgcctccgt ccgaccgcgc caagctgttg gccagcctga
     1381 ccgatgcgtt tacgaaaacc gtatccgcca atgcgcgtgt gatgccggaa acgtcaaaac
     1441 tggcgacggc tttggaattg attgagttct tgatggcgtt tgtgcaagaa aaacatccca
     1501 aacatttgcc tgcctttgga ggtattggag tcgtttgggg cggaagtgga gaagaagttt
     1561 ggttaatagc cgtctgtcaa tctcccataa cgtgatcctc ctactgaaaa tacttcaaca
     1621 tctgacttta aacgctctcc caatattatg gtttggcaac gggaacagga atatttatga
     1681 aaagcacctt catacccatt gaattgaaga atcgatttga caccctgctc gtaacattgg
     1741 ggacaaatat catgaattgg ctcgctgttc gccatctctg ttttcaatcg atagacgaaa
     1801 gaaccaggag caagttcata caattggtag cgttgcttct ctgtagacca attttttact
     1861 gccaccagtt ctttttccaa ctcatctata cggctaattg ccgccgtata agaagattgg
     1921 gcttcaaata aatcagtttg aataccagca atgatttgat taagttcgat ggttttttgc
     1981 gctacctctg cctctgtttt gagctggtta aaccctttta aaagtgatcc tgcttctttt
     2041 aggcctataa atgcgttaga aatgaatcca gtcattataa atactcatga aaacaaaaga
     2101 attcctcaaa tcccttgccg aactggccgc cagtttgcgc caagtcatcg aagcggaagt
     2161 ggacggcttt gatgcgtcgc ccaaggctat tgctgcacgc cgtgccaagg tgtttgaccc
     2221 ggtaggcggt tacgagtatt tcgtcaacac ctactttccg cattatatcc gctcccctga
     2281 aaaatccgaa ctgcatgcgt ttttattcag ccgtctgccg gagattatcc gctcccccaa
     2341 aggggaaaat gaggcggtgg gtgcgccgcg tggcgagggt aagtcgacgc aggttactca
     2401 gttgtttacg ctgtggtgta ttgtgacagg ccaaaaacat tatgccgtta ttgtgatgga
     2461 cagcatagat caggcatacc cgatgctcga agccatcaag gcggaattgg aatttaatcc
     2521 gcgcttgaaa accgactttc cggaagtatg cgggcagggc cgcgtatggc aggccggtac
     2581 gattgtgacg gccaatgacg ttaaagtcca agtggccggt agcggtaaaa agctgcgcgg
     2641 tttgcgtcac ggcccttacc gtcctgacct gaccatattg gacgatattg agaatgacga
     2701 gcaagtccgc aaccccgaac agcgcaacaa gctcaatgcg tggctgacta agactgtatt
     2761 gcctttgggc ggtgtcggcc agaaatacga tgtgatatat atcggcacga ttttgcatta
     2821 cgacagcgta cttaaccgca ctttgaataa cccgttttgg cacggtatta agtttaaggc
     2881 gatgaaacgc tggcccgacc gcatggattt gtgggacaga tgggaggaac ttttccgaaa
     2941 cgacggcgag actgtggccg aggcgtttta tctcgccaat aaagacgaga tggagcgcgg
     3001 tgcgcaaaca agctgggcgg ctcgcggcgt actcgcgctg atgaaaatcc gtgcgcgtga
     3061 cggtcatgcg acatttgaca gcgaatatca aaacgacccg gtcagcggcg aagatgcgcc
     3121 gtttgccaag tcgatgaagt tttggaacga cctgccgtcc gatttggtgt atttcggtgc
     3181 gctcgacccg tcgctgggta aagcgggggc gagccgtgac ccgtcggcga ttatcattgg
     3241 cggttatcaa cgtgaaaccg gcaaactgta tgtcgtggaa gctcagatta aaaaacgtct
     3301 ccctgatttg attattgagg acgttattcg attgcaccgt caatatcgtt gcaaactgtg
     3361 gtttgttgag acggttcagt ttcaggaatt tttgaaagat gagctggtca agcgcagcgc
     3421 ggcgcgtgga atacctgtcc cggcgcgggc ggtcaagccg gtatcggaca agctcttgcg
     3481 gattgagact ttacagcctc acatggcgaa cggtttgatt ctgttgaatg agagccaaca
     3541 gacgctgata cagcagttcc gccattttcc aaaggctgat catgatgatg gtcctgatgc
     3601 agtgcatatg ctctggtcgg gggcggtggc caattgtgtg ccgatagaat ggcaaagccc
     3661 taccgataac gattttgatg acgagataaa aagtaaatgg agccgataat ggcaaaaaag
     3721 aacaataaaa ctaaaatcca aaagcccgaa gctgcattgc agacggacgt ggctcaaatt
     3781 acggcgaccg gtcgggttat cgccgagcat ccgtccaatt ttattacacc gcaaaagatg
     3841 cgggccctct tcgaggatgc ggaaagcggc gacatccgcg cccaacacga gcttttcgcg
     3901 gacattgagg agcgcgacag cgacatcgcg gcaaatatgg ggacgcgcaa acgcgcgctg
     3961 ctgacgctca actggcgcgt cgccccgccg cgaaatgcga cgcccgaaga agaaaagctg
     4021 tccgaccaag cctacgaaat gatggacagc ctgcctaccc tcgaagacct gattatggat
     4081 ttgatggacg cggtagggca cggattttct gcgttggagg tcgagtgggt attttcagac
     4141 ggcctttacc taccccgaaa ctttatccac cgcccgcaaa gctggttcaa atgggacaaa
     4201 gacaacgggc tgctgctgcg tacccgcgaa aatccggaag gcgaagcgtt gtggccgctg
     4261 ggctgggtcg ttcataccca aaaatcgcgc agcgtccagc aggcgcgcaa cgggcttttc
     4321 cgcacgcttt cctggctgta tatgttcaaa cactacgccg tccacgattt tgccgagttt
     4381 ttggagctgt acggcatgcc catccgtatc ggcaaatacg gcgcgggcgc aaccaaagag
     4441 gaaaaaaaca ccctgcttcg agcggtggcg gaaatcggtc acaacgcggc aggcatcatg
     4501 ccagaaggta tggaaataga gctccacaac gcggcaaacg gtacgacggc aaccagcaat
     4561 ccgtttttgc agatggccga ctggtgcgaa aaatcggcgg cgcggctgat tttggggcaa
     4621 acgctgacca gcggtgcgga cggaaaatcc agcaccaacg cgctgggcaa tatccacaac
     4681 gaggtacgcc gcgatttgct ggtgtcggac gcaaaacagg tggcgcaaac catcacaagc
     4741 caaatcatcg gaccgttcct gcaaatcaac tatccccatg ccgacccaaa ccgcgtgccg
     4801 aaatttgaat ttgacacgcg cgagccgaaa gacatcgcgg tctttgccga cgctatcccg
     4861 aaactggtgg atgtcggcgt acaaatcccc gaaagctggg tgcgcgacaa actggtcatt
     4921 ccagatgtgc aggagggtga ggctgtgttg gtgcggcagg taccggacaa tccggtaaac
     4981 agaactgcat tggcggcttt atccgcccac accgtaccat ctaaggctac gggcaggcat
     5041 caggaaatat tggacggcgc gttggatgac gcgctggttg agcccgattt caattctcag
     5101 ctcaacccga tggtgcgtca ggcggttgcc gcacttaatg cttgcaacag ctacgaggag
     5161 gcagatgccg cactgaatgc gctttatccg aatttggaca acgcgaaact gcgtacctat
     5221 atgcagcagg ccttgtttat cagcgatatt ttgggacaag accatgcccg cgcctgattt
     5281 gggatttgcc ttaagtctgc cgccaaaaaa ggcaatcgag tggctggaaa gtaaaaaggt
     5341 tacggcggag agctaccgca atctgacagc ctccgaaatt gccaaagtct atacgattgc
     5401 ccgcatgacc gacttggata tgctcaacga catcaaaact tcgatggttg aatcggcaaa
     5461 aagtggacag tcgtttgacg attggcgaaa aggtatcttg aatctgctca gcaacaaggg
     5521 ctggctgcat ccgaacgggc ataacggtaa ggatatcatc gacccagcca ccggcgaggt
     5581 attcggttcg ccgcggaggt tggagacgat ttaccgtacc aatatgcaaa ctgcctacaa
     5641 cgccggtcaa tatcaaggat atatggcaaa tattgatgca cgaccttatt ggatgtatga
     5701 cgcggtaggc gacagccgca cccgtccggc gcattcggca atagacgggc tggtgtaccg
     5761 ctacgacgac ccgttttggg caacgtttta cccgcccaac ggctacaact gccgctgctc
     5821 ggtcatcgcg ctgtcggagc gggatgtgga acgccagggg cggattgttg ggcaaagcac
     5881 ggcggacaat ctggtcgaga cccataaaat ctacaacaaa aaaggcgata cttatctgac
     5941 ccttgcctat aaagcaccgg atggcagtct gtacacgacc gatcgaggat ttgattacaa
     6001 cgccggacga atgaactacc gccccgattt agacaagtac gaccgtgcgt tggcgcatca
     6061 atttgccaaa gcggaaatgg gtggtgcgga ttttaaaacc agctttaaac agcttgaaaa
     6121 agagttttat gaagtcaagc aacgtttgga tattgatggc aagcccgata aagagcagaa
     6181 aatcaaaatc cgaaatgcgc tatcaagaca gcttaaattt gctgcgggtg tattgagcaa
     6241 ggaaacgcaa gaattggcag gtatgacacg agcgacggtg tggctgtctg atgatacgtt
     6301 ggttaaacag gtagacagcc gtgaggggca gaatttcgat gactcctact atgctttttt
     6361 gccggatatg ctgcaaaacc ctgaacatgt catccgcgac aatcgtgaat tgattttcac
     6421 agctcgctat aaaggctcgg cattgtgggc agttttaaaa tatattaagg aggtggatga
     6481 gatttatcta cagtcgtacc gaatcagtaa cgacaaagag attgccaaat ttatggcgaa
     6541 gaagaaagta ttgaaataga cgttgggcaa ggctcgaaat cacttgcaca cgctctcgga
     6601 cgccctaacg ggcaggctgc ggtatcgagg ttatcaccgc ttttccaacg tctgtacgga
     6661 ataataccat gattgatgtc aaaatagaca atatctttgt cgtcctaaac caaatcgagc
     6721 ggcttggcaa cgggatcgaa aaccgctacc tgctgatgcg ccgactgtcc gaaaccatgc
     6781 acacggcggt caagctcaat ttccgctacg caggccgtcc gaaatggttg gggctaaaat
     6841 accgcgacgg caagccgctt tcggattcgg gtcgtctgaa agacagtttt tccacactgt
     6901 cagacaacga tacagccctt gtcggtacga atatcgtcta tgccgccatc cacaacttcg
     6961 gcggtatggc ggggcgcaac cgcaaagttc ggattccgca acgggaattt ttgacgctga
     7021 cggacgacga caaacaggct ttgatggacg atgtgcagga ttatttttcg ggtctgatac
     7081 cgtgaattta taaaaccctc aaaaacgcgc tttttagcgc gtttttttat gcgggtaata
     7141 caaacccctg cccaagatat aaaaatcaat cctagacgct tctaaaaagc ccctgaaaac
     7201 gattaattgt gtatcgcgcg gacaggtttt aaaaaaatgg cgggagggtt tgaagcacgc
     7261 ctactctttg ttgttttttc aaataggcaa aatgaccgta ttgagagagg tacacatgtc
     7321 caaaaatgca caaaaaaccc tacttgccgt gtgcagtttc gaggtgcagc caaaagacgg
     7381 gcgaatccaa ctgctgccat atggcgaatt tcgcgcagta gacggtcgtc cgactgatgt
     7441 ccctgcgtgg tatctgaccg aagaaaacgg tcatgatgtc gcgttgttgg ccaacagctc
     7501 gcgcaatcag ttggttgtcg attatgaaca ccagacgctc tacaaagaga aaaacggaca
     7561 acctgcacct gccgccggtt ggatgcgttg gctggagttc acgcctaaag gcatgtttgc
     7621 cgaagtggag tggacggaca aggcggctgc ggcaattgcc gcaaaagagt atcgctacat
     7681 ctctgctgtg ttttcctatg acacaaaggg atatgtaagc aaaatttttc acgccgcgct
     7741 gacaaatttc cccgcgttgg acggtatgga cgaggtgctg gcggcagcgt cggcgcaaat
     7801 tttaaaaccg gaaacggagc aaaaccctat gaaagagttg ttacagcaac tgttcgacct
     7861 gcctgatgcg ggcgaagaag aactgaaggc ggcattgtcc gcgctcgtgg aagccaagcc
     7921 gaaagacgtg gcattgtctg ccgacgtgtt cgcgcagctg gcggaaaaag acagccgcat
     7981 cgcggcattg acggcgcaaa ccgccaagcc tgatttgact aaatacgcgc ctatctcagt
     8041 ggttcaagag ctgcaaagca aagtcgccgc gctgactgcc aagcaggaag cagacaaagg
     8101 caacgaattg attaccgccg cgctgacttc aggcaaattg ctgcctgctc agaaggagtg
     8161 ggcaaaaggc gtattgaaac agccgggcgg cttggcattt ttgaccggct ttattgaaaa
     8221 cgcccagccg gtcgctgcac tggcaggctc gcaaacgggc ggcaaagcac ccgacgaacg
     8281 cgtcgccgca ctgactgcgg aagaggcagc cgcagcaaaa atgctgggca tgtccggcga
     8341 agaatttgta aaaatcaaag aaagcgaagg taagtaatgg acaaatcagc gattttgacc
     8401 gcaatcacgg cagcattccg caaagaattt caaacggggg cctgcaaaac cgcctgaacc
     8461 ggttaaccgc caaacaggtg cagccggtag cgcaggcggt gcgcctgtta tccacgtcat
     8521 cgctgttgtc ggtggcaacg gcattaatcg aggcgcatgg cgaagagatg accgcgcccg
     8581 atttgattga ggttaaccgc gccatgcgcc gccgtatgca ggccgagatt gccgccttac
     8641 gggcggtgca gacggctgct gccgagtctg gtgggctgac ggccaacgcc gtgtataccg
     8701 aggcttacca aacggcagaa tccctgcgcg cggcggcagg ccgtctgaat gcgttggttg
     8761 cggcggtcat caaccaaaag ccgccgctga ttgtgcgcca agccccaatc gacggtacga
     8821 tacaccaaat cgcccacgag ttttacggcg atatagcccg cgcagcagag ctggtgcggc
     8881 tcaatcccca tatccaccac cccgcgttta tcaagcgcgg cactttggtc aacagctatg
     8941 caaaataatt catacggcta tgccgtgtcg gtgcgcgtgg gcggtaaaga gcaccgccac
     9001 tgggagcgct acgacatcga cagcgacttt ttaatccctg ccgacagctt cgattttgtc
     9061 atcggcaggt tggggccgga ggcggccata cccgatttaa gcggagagag ctgcgaggta
     9121 gtgatagacg ggcaaatcgt gatgacgggc atcatcggca gccagcgcca cggcaaaagc
     9181 aagggcggcc gcgagttgag cttgagcggg cgtgatttgg ccggtttttt ggtggattgc
     9241 tccgcgccgc agctcaatgt aaagggcatg acggtattgg atgcagccaa aaagctggcc
     9301 gcgccgtggc cgcagattaa agcggtggtg cttaaggtcg aaaacaaccc cgctttggac
     9361 aaaatcgaca tcgagccggg cgaaactgta tggcaggcat taacccatat tgccaactcg
     9421 gtcgggctgc atccgtggct ggagccggac ggcacgttgg tggtgggcgg tgcggattac
     9481 agcagcccgc cggtggcgac attgtgttgg agccgcaccg acagccgctg caatatcgag
     9541 cgcatggaca ttgagtggga taccgacaac cgcttttccg aggttacttt tttggcgcaa
     9601 tcgcacggcc gcagcggcga cagcgccaaa cacgatttaa agtgggtgta caaagacccg
     9661 acgatgacgc tgcaccgccc taaaacggtg gtggtgtccg atgccgacaa tttggccgca
     9721 ttgcaaaagc aggctaaaaa gcagctggcc gactggcggc tggagggatt tacactcacg
     9781 ataaccgtgg gcggccataa aacccgcgac ggcgtattgt ggcaacctgg ccagcgtgtg
     9841 catgtgatcg acgacgagca cggtatcgat gcggtgtttt ttctgatggg gcggcggttt
     9901 atgctatccc gcatggatgg cacgcaaacc gagctgcggc tcaaagagga cggtatttgg
     9961 acacccgacg cttaccccaa aaaggccgag gcggcgcgca agcgcaaagg caaacgcaaa
    10021 ggcgtgagcc ataagggcaa aaaaggcggc aaaaaacaag cagaaacggc ggtgtttgaa
    10081 tgagtttgag taaattggcg aaaaaaacgg cacaaactgc taaaaatatc ggcgaaaccc
    10141 tgcgcgcggc ctttcgggga aaaatcacgc tggtggtgtc gtccgagccg atacagcgcg
    10201 tgcagttgag cggcttggcc gacgaaaccc tgcaagacct tgaacatttg caggaatacg
    10261 gctttgccag ccatccgccc gacggcagcg aagcggtagt gataccgctg ggcggcaata
    10321 cttcgcacgg tgtgattgtg tgcagccagc acggcagcta ccgcatcaaa aaccttaagc
    10381 ccggcgagac ggcgattttt aatcatgagg gtgcaaaaat cgtgattaag caaggcaaaa
    10441 tcattgaggc cgattgcgac gtgtaccggg ttaactgcaa acaatacgag gttaatgcgg
    10501 ccacggatgc caaatttaac gctccgttgg tggagaccag tgcagtgttg acggcgcaag
    10561 gccaaatcaa cggcaacggc ggcatggccg tcgagggcgg cgacggagcc acctttagcg
    10621 gcgatgttaa ccaaacgggc ggcagcttta acaccgacgg cgacgtggtg gccggcaata
    10681 tatcgttgcg ccagcacccg cataccgaca gcatcggcgg caaaacctta ccggcggaac
    10741 cggcatagac aagcagacct ttggcagcct tcgggctgct ttttttgtgc gtgtgggatt
    10801 gaagcccgtg tactccgtga ggccgtctga aaacggcaaa atgccaacat ggacaaagag
    10861 ctaaacccca gcatcggcga ctataccggc cgcaccgtcg atacgctgca aaatgccgtg
    10921 tatatccgct tgatgacacc gttgggcagc tggtgggcgg ataaaacgct cggctcgctg
    10981 ctgcatttgt tgcagcgcga aaaagacctg caacgggtca gcctgttggc cgagcaatat
    11041 gccgatgagg cactgcaacc gattgttaag agcgggcgtg ccgacaagat taccgtgcgc
    11101 gcagagcagc cgcacgacgg ccgcctgatc ctgcatatcc gggtggatac ggcggcgggc
    11161 gggtttgatt accgccacga agtgcccgtg atttaaagag gttttaaacg tgtttgaaac
    11221 gccgacattt gagcaaatcc gcgagcgtat cctgcgcgat accaaaagcc tgtggccgga
    11281 tgccgatatc agccccgaca gcgaccatta tgtgcacgcc agccgtttgg ccagctgcgc
    11341 cgaagggcaa tatgcgcatc aaagttggat tgtgcggcag attttccctg ataccgccga
    11401 ccgcgagtat ttggagcggc atgcctccat gcgcggcttg cgccgccgca atcctaccac
    11461 ggccagcggc acgctgaccg taagcggtat tgcgcaatcc atgctttcag acggcctgca
    11521 agtgcgtatc ggccagcgtt tttaccgcac taccgcccgc gccgttatcg gcagcggcgg
    11581 cacggcggaa ataccggcaa tcgccgacga gccgggcgcg gccgccaatg tggcgacggc
    11641 gaggcgcaac tgatggccgc ccccgccggt gtggccaccg aatgccgcct taccgtacaa
    11701 ggcggcaccg accgagaaag cgatgcctca ctgctggcgc gtctgttgga aatcatccgc
    11761 cgaccgcccg caggcggcaa ccgttacgac tataaaaact gggcgttgag tgttgacggc
    11821 gtaaccagcg catatgttta tccgctgcgc cgcggcttgg gtacggtgga tattgccatt
    11881 acctccgccg acggtgtgcc atcggaagaa actgtgcgcc gcgtacaggc ttatatcgac
    11941 gagatgcgcc cggtaacggc aaaaaatgcg ctggtactca agccaaccgt aacggcggtg
    12001 cctgttaccg tgcaagtcaa gctcgacggc atcgacttgg acgaggccaa gcgccgcata
    12061 cggacggccc taaaagaata tttcgacacc ctgatccccg gcgacggcct gactgtgtcg
    12121 caaatcgagg cggctatcag caatgtggat ggtgtgatcg accgccgtct gactgcgccg
    12181 acggccaacc gtgccgccga tacggttaac cgcatcgagt ggtttaaagc gggcgcgatt
    12241 aatgtaacgg agatgccgtc atgagctatc aagacatctt gcggggcctg ttgccccccg
    12301 tgtcgtatgc ccgcaatgcc ccgcgtgtgc gggcgcaggc agaaatagac ggcgcagcgc
    12361 tggatgcggt ggcggaatcg gctcaaagcg ttgccgatgc cgtcgacccg cgcagcgccg
    12421 gccaaatgct ggccgattgg gagcgcgtat taggtttgga cggtacgggc aaaaaccgcc
    12481 agcgccgtgt gttggccgtc atggccaagc taaacgaaac aggcggcttg agtattcctt
    12541 attttgtgcg tttggccgag gcggcgggct atcaaatcca aatcgacgaa ccgcagccgt
    12601 tccgcgccgg tgtaaaccgc gccggcgacc gtcttgcgcc gcaggaaatc atgtgggtgt
    12661 ggcacgttaa cgtgcgcggc ggcaacaacc gcattacccg attccgcgcc ggtatctcgg
    12721 cggcgggcga caggctgacc gattacagcg atgccgtgat cgagagcctg ttcaaccgcc
    12781 tcaagcccgc ccacaccgct atccgattta cctaccgata aggactaaac catgcacccc
    12841 atcgaaaccc ccgacaagac cttccacgac ggcgacggcg tgtccgaatt gggcaccatc
    12901 ctgcccgcgt ggtggctcaa ccaagtgcaa tccgagctgc tggccgtgct gactgcggcc
    12961 ggtatccagc cggataagtc gcagcctaat caattactgg cagcactaaa taggctggct
    13021 gtagttacca ccggcgacca aaccatcacc ggccaaaaaa cctttaccgc ccaaacccaa
    13081 ttccaaagcg gcatccattt atccgccaac cagacgaact ggaacggcgg ccacaaagcc
    13141 tacatcggcg cggatgccga caacgcccac atcgtcttcg gcgacgacac cctccgcctg
    13201 cacggcgcaa acaaccgcat ttcctacaac aaccacgaca tcttccacaa agccaacaaa
    13261 ccgcgttttg ccgaagacat cgaaggtaag ccgaacacac tgtccggata cggcatcggc
    13321 aatttcaaag tcgaaacctt ccggggcgat ttgaacaccc tcaaaacaga cggcatctat
    13381 tccctgccga cggcgggcgg cagctccaac ctgcccgttg aaaacaccgc ctgccatatc
    13441 caagtcatcg ccggcacgaa acacggctgg tgcaggcagt tgggttatcc cgcctacacg
    13501 tccgacgtgt acgaacgcca ccaaacgagc agcgcaaacg acaactggtc cgcatggaaa
    13561 aaactcaatt cggacggcat ccccgtcggc gcgatcgtat cctttcccaa agccgtccga
    13621 aaccccgcag gctatctcaa agccaacggc acgacctttg cacaaaacac cttccccgac
    13681 ctctaccgcg ccctgggcaa cagcaaccgc ctgcccgatt taagccgtac cgacatcggc
    13741 atcaccgcat ggtttccgtc cgaccaaatc ccgaccggct ggctggcgtt tgacgacatc
    13801 cgcacgcgcg taaccgaaac cgcttatccc gagctgtacc gtctgctgac cggaaaatac
    13861 ggcagcatcc aaaacgtccc gcaggcggaa gaccgcttta tccgcaacgc cgccaacggc
    13921 ttggcagtcg gaacgaagca ggaagacgaa atcaaacggc acgtccacaa agtattttca
    13981 cactgggtaa accacccgca cgcggcagcc ctcggttacg aagaccacaa cgaaaggcag
    14041 agaagcgcgc tcgtatcgac ttggacggac gaaaatttaa acgacaacgg ctttttaacc
    14101 ccgcgcccgg acagcaaaat ggcgacaggc ggcgacgaaa accgccccaa agccctggtt
    14161 ttaaaactgt gcatcaaagc cgccgacacc ttgggcgaag ccgtgttctg gataaagtcc
    14221 cacggcgaaa ccgtcaacgc cggcgcgctg gacgcgggca cgctggcgca aaacctccaa
    14281 gacaaagcag accgcgccca cacccacacc gccgcccaaa tccaagggct ggacgaaaaa
    14341 atcagcaccg ccgttgccgc gcaattcaca cgccaaacca tcggcggcgt ggatattgtc
    14401 agattccccg acggcacaat gatacagacc ggcagttaca ggttcacacg aagcggcagc
    14461 cccatagaaa acgaagtcgt cttccccgtc gcctttgccg acggcaacgt caaatgcttc
    14521 gtatccgaac gccattcggg acgcgtcacc ggcgatcgaa ggcaacacaa ctggctgttt
    14581 atccgcgcaa aaaaccacgc cgccgccatt attaccaact ggtacgaagg cagttgcgac
    14641 tggatggcca tcggcaaagc cgcctcggga aacgccgcca gctccctgat agtccccgaa
    14701 atacctgaaa ccaacgaaga accgcaaaga gagagtggaa gaacatcaac cggaccccga
    14761 aaccgccgcc gccaccgaga cggcttgctc gaggcactgc aagactagcg ggctgtagag
    14821 atggctgtag agatgggcag tagagacggg ttgtagagat gggttgtaga gatggctgta
    14881 gagatggctg tagagatggc tgtagagatg ggctgtagag atggctgtag agatggctgt
    14941 agagatggct gtagagatgg ctgtagagat gggctgtaga gatgggcttc agcccgccga
    15001 tccaagcaat ccgaccgaaa ccggccgcgc cgccaatccc ccgaaaccta tgccccgcca
    15061 atcctgccac tcttcgtcat tcccgccgct ttcgtcattc ccgcgaaagc gggaatccag
    15121 cccctaaacg cggcaggaat ctatcggaaa aaacagaaac ccccgccacc gtcattcccg
    15181 cgaaagcggg aatctagacc ccaaacgcgg caggaatcta tcggaaaaaa cagaaacccc
    15241 cgccgccgtc attcccgcga aagcgggaat ccagaccccg acgcaacagg aatctatcgg
    15301 aaaaaacaga aacccccgcc gccgtcattc ccgcgcaggc gggaatccag accccaaacg
    15361 cggcaggaat ctatcggaaa aaaccgaccc cccgccaccg tcattcccgc gcaggcggga
    15421 atccagaccc caaacgcggc aggaatctat cggaaacggc tgaaaccgaa cggactggat
    15481 tcccgcctgc gcgggaatga cggcggcagg ggtttcggga ttcccgcctt cgcgggaatg
    15541 acggaaagtg gcgggaataa cgaaaggcgg gaatgaccgc gcaaaaagcc gctgccccct
    15601 tcggacggca ccggcaacaa aaaaccgcac ggccgaaacc gcgcgggaaa ggatagtcgg
    15661 gcgcgcccga taagcagcgg ccgcccccgt tatttcaatt gggcgacata ttggcgcaaa
    15721 acctcgttga tgcgcgtctg ccagcccttg ccgccggcgc ggaatttttc gaccacatcg
    15781 gcggacaggc gtatggtaac gagttgtttc ggggttttgc ctgtgtttcg tttttgcatt
    15841 acgccctttt cttccaattg tttttgatgg gaaaaaagca cctgtgccaa gtcttcgggc
    15901 agggcttcgg caatggggcg ggcaagcgca aaatcttcgg cggcaagttc ccgcacttcg
    15961 ccgtcagcgt ttgttaagga ttgacgttgc atatttttta acctctcttt tattcgcttt
    16021 gcgaaaactg atgacacgga tgccgtcttt tatcggcgta aaacagacaa tgtgcaggcg
    16081 ttgcgtatcg cctagataag cagcggcaac ataacgcggt tcggggtaat caaagcagac
    16141 atcgggcaca ataacggccg ttgtccagcg tatttgcccg actgattcaa agggcaaatt
    16201 ccgctcttcg atattgcgtt gatttttttc ggagtcaaat tcaatcttca ttgcagcttg
    16261 cagcgtattt tgtcgttaca ttataagcgg caaaaaacca aaatgtaaat acaaaaaagg
    16321 aaaccccgaa atgaccatct atttcaaaaa cggcttttac gacgacacct tgggcagcat
    16381 ccccgaaggc gcggttgccg tccgcgccga agaatacgcc gcccttttgg caggacaggc
    16441 gcagggcggg cagattgccg cagattccga cggccgcccc gttttaaccc cgccacgccc
    16501 gtccgaatac cacgaatggg acggcaagaa atgggaaatc ggcgaagccg ctgccgccgc
    16561 ccgtttcgcc gaacaaaaaa ccgccacggc attccgcctc gcggcaaagg cggacgaact
    16621 caaaaacagc ctcttggcgg gctatcccca agtggaaatc gacagctttt acaggcagga
    16681 aaaagaagcc ctcgcgcggc aggcggacaa caacgccccg accccgatgc tggcgcaaat
    16741 cgccgccgca aggggcgtgg aattggacgt tttgattgaa aaagttgtcg aaaaatccgc
    16801 ccgcctggcc gttgccgccg gcgcgattat cggaaagcgg cagcagctcg aagacaaatt
    16861 gaacaccatc gaaaccgcgc caggattgga cgcgctggaa aaggaaatcg aagaatggac
    16921 gctaaacatc ggctgaaaaa atacgtttac cacctgttgg tagccatcga ccaactgttc
    16981 aacgccctaa ccggcggcgc ggcggacgaa accctctcaa gccgcaccta tcgcggcgcg
    17041 cggctcgccc aaaagcccaa aacccgctgg aaggttttat acaccctgat caacggcgta
    17101 tttttcgacc gccaacactg ccggcaggcg tatatcagcg aactgaaagg caggcagcac
    17161 gacgcgcggt tcaaccaaag ccgcgccgcc ggggaaaagg ggacgcgatg aactacttcg
    17221 gcatggtaga gtttctgcgc ctgatggcaa tggtgcggcc gccatttgtc ttcttctccg
    17281 gcacccgcag cgaactgcct acttatcttg acctcgtggc agaactgcgc ctgaccggat
    17341 gggagcgttt tgcaggcagc caaaccttga ctgtgagcag cacatcaaca gcaattcaag
    17401 ctacgacgac cacctgattt ataagttctg accgcaagta gcgtactact tttaaaggca
    17461 taagataatc cccgtttaac agaccattaa acggggataa atttgtgcaa aagctaatac
    17521 aatttcctac gcttcggcgg tgcaaaagct gccgccaatt cgtgcaaaag ctgccgccgc
    17581 cttacattcc agtgcaatgc cgtctgaaac ttcgctaatc tcgggttgcc gcgcgctgtg
    17641 ttgttcttcg gtactcagca aaaagccgta cagacgctcc aatcgggcgc ggtaggcggt
    17701 gaatttgttg tagaacatcc ggaaaaaaga tagcgcgttt tgcagtcgcg caaaggcttg
    17761 gacggtctgc tggatgtcgc cgattttgat ttgtccggca aacaggcgcg gcgcttgcaa
    17821 aataatgggg aagagtttga tgccgttggt gaacatatcg ttaaagccgc ttaagcagac
    17881 gctttgccgc gcgatgcgcc aacggttgcg gataatggct ttaaagcggt cggaaagccg
    17941 gccgtgttcg tgttgttcgc cgttgtaaaa cgctacgctt tcggcgtggt cgcgcacgcg
    18001 gatgagggaa taacggtagt cgccgttgag tttttcgttt tcataattgt aacgaatcaa
    18061 agggttgcct atccacatgg cgataaaggt cgccaaaatc acaaatatat agacaaacca
    18121 aacgatgccg tgcggaatgt cgaagccgaa cacggtcagg atgccagcca agccccgcaa
    18181 aacaacggca aattccagag aagtaacgac cgaattgacc atgccgcgca caaattcgat
    18241 ggtcgaagcg ataaattcct gcgcgtcctg ttggatacgc tggtcgatgt tgtctggcgc
    18301 gtggcggcgc atttgcaggc ggtagtagtt tttgtcggca agccagcgtg ctgtcagcac
    18361 ttcgttgagc cgctccgacc atttaatcgc caagccttga tcgaggaagt cgttgacgac
    18421 gttgttaaac gcccgtatca gcaccacgcc cgcgttcatt gcagcaaaca tccaaaatgc
    18481 cgaagcattt caaatcctgc atcgagtcgt aaagcccttt ggacataaag gtactcaaca
    18541 cattcagccg catttcggtt aacaccagcg taatcatcgc cgtaatcagc agcaagactt
    18601 tgaccgcgct tttcagtgtc agacaaagcc aaagcggtgt ggaataaagc tcggtttgcc
    18661 atttctgcat gggaaatttc ttacggtatc aatgccgtct gaaaaagacg ggtacagttg
    18721 attttttgat gaagtttggg gaagttttgc aggtcagggc atattgcgtg ttaatttacc
    18781 atacaaccac gccggaatta agtttaaatt tgaataaaag gttcgggttc tgcaaaatac
    18841 agaacccgaa ccttgttcgg atattgaaac cggccgcccg attttgggcg gtgcggcttg
    18901 caagtatcaa gattcgcata tgccgtctga agctcggaga ggttcagacg gcatatgctt
    18961 atttgggctg ctcttcgacg aatctcggac ctttcaagat gccgttgtga gaatagggcg
    19021 acagcaggtt gtatgcggcg gttttggaaa cctgataacc gcggtcggtc aggctgttgg
    19081 caatctgatt gaccactgcg ctgaccaaag cccccaacag gccgctgttg ctgttgttgc
    19141 tgccttcgcg gatgctggcc gaacccgacc acaactcttt tccgttgcgg gaatcgacca
    19201 gccgtgcttt ggcggatacg gtcgtcacgc tgtctaaaat ttgatatgaa gtgccgtatt
    19261 cggtaaccgt aatgtacaaa accgcatcat tgccgaaaat ctgatgcagt ttttccggcc
    19321 ggacggcgtg aatatcggcg gcattggtca agccgttttg tttgaaggtt tcctccacga
    19381 ctgcggcggg gaagacgtaa tagccggctt cggaaagcgg cgcggcggtc gaagccagta
    19441 caccccatgt tccgttgaca tcgggcgatt cgttcagcgg cggaaccacc aaaattgaag
    19501 ccggtttgct ttccttgaat gacgtgtagt cgaaatcggg cgctttttga acttggcagg
    19561 cagacagcgc caacacggcg gcaagcccta aaatcaaagg tttcatcgct tgcctccttt
    19621 accggttttc atcaggaagt ccataaatac gcccgattcg ggaaacagcc ttttctcttc
    19681 ttcaaactgg cggaacgcgc cctctttgtc tcccgaacgg gaaagcagca gtcccaaatg
    19741 ggcgtgcgca cccggggcgg cattcatttt tttgttgccg gcttccacaa agtatttttc
    19801 catcttttcg gtctgcttgc ccaacgaagt gtcgtcgttt ttcaaacctt catagacggt
    19861 atcgggatag ccgccgtaat aatacaggga tttttgcccg ttgccgccgc aggcggtcag
    19921 agccaagacc gccgcacaca gcgacaaacg gctcaaggtt ttcggattca tcatttctcc
    19981 ttaacggttg ggttgccatg cgccgttgtc aacagcctga accaggctgt tgacggcttc
    20041 gcggattgcc aagtctaaaa ctttgccgtt caaagtcgca tcgtagccgg aagtgccgcc
    20101 gaaaccgatg atttcacggt tggaaagtgc gtattcgccc gcgccctgtg cggaatagat
    20161 gatttcggaa gtattgacgt tgacgatatt cagagccact tttgcatagg cgatttgcga
    20221 tttgccgcga cccaaaatgc cgaagagctg atgatcgccg acatctctgc gtccgaattc
    20281 ggttacatcg ccggtaacga cataatctgc gcctttcagg ttatgcgctt tgccggaaat
    20341 gccggattcc tgttttaatg cgttcaaatt ggtgcggttc agtacgttga agcggttggt
    20401 ctgttgcagg tgcgttacca gaatggtttt tgcctggctg cccaaacggt cttccccgtc
    20461 ggagaaaatg cctttttgga agctggagcg gttgtcgaat gttccgacgg aaatcggggt
    20521 acgaacaccg tgatattgcg tattgtagga ggcgactttc tctacctcga gactgcgtga
    20581 ggattcggtc gcacagccgg tcagtgaaac ggcagcggcg gcaaggacaa cggcggtgga
    20641 aacggttttc ataaaattta ccctaaggtc aagttaagga aataacgggt tatcattatt
    20701 gtccttatgt aaatttaagt caaggtgttt gtctgtgcgg gacggatgcg cgcggaaggt
    20761 acggatattt ttcaaacgga atcgcggcgc gggcgggatg cctgcctttt ccggatttag
    20821 aggtaaacga tttcgccact ccgccctttg ctttcggcac ttgcccacca gacaaatgcg
    20881 ggcagcacgt ccccgtagct tttgcgttcg cttttggctt cgcccggatg ggatttgatg
    20941 cgttgcgggg aattgatggg gccggggacg aggacgttgg cgcgcaggtt gccgaagcgt
    21001 tcccattcgt cggcggcgac tttgcacagg tagttcaacg cggctttgga cgcgccgaag
    21061 ccgccccagt aggctttggg tgtttcgccg tggctttcgc cgacgaagat gacggacgcg
    21121 tcgggcgact gcttcagcag cgggaacagg gcgcgggtca gccccatagg tgcgacggtg
    21181 ttgatgcggt attggttgac ccattcggcg acggtttgga aatccagcgg cgagagggcg
    21241 taaaaatagc cggcgcagtg gacgatgccg tccagtttgc cttgcgtggc ttcggcaatg
    21301 gtggcggcga actgattgaa ttctttttct tccgcactca tcaggtcgaa acggatggcg
    21361 aagggttcgg ggtgtccggc ttcgacaatc gcgtcataga ctttttccag ttttttctga
    21421 tggcgtgcca ccagaatcac ggttgcgcct gccgccgcat aggctttggc gacctgttcg
    21481 cccagacctt gcgatgcgcc ggtaattaag atggttttgt cggacagtgt cgccatactt
    21541 tttccttttt ggtggtcggt cgggggtatt ttagcgtttt gccgcacctt gtaaagcgtc
    21601 ccgatgccgg ccggcgtttt cagacggcat cgctcaggct ttgcaggcag gtttccgccg
    21661 ccgcgaaacc tgattgtacg gcggcttcga gcgtggcggg gtagtccggg tggaggtagt
    21721 cgccggcggg gaagatgcgg tgccggtgca gccacgacaa atccggcggc ggggcatcgg
    21781 ctgcggttgt ggcgcgtttt tcggtgatga cgcgcacggc ttcgggttcg cccaaatgcg
    21841 gaaggatgcg tttgaggtcg gcgtgaactt tgtccgccca cgctcggttt gtaaacgcgc
    21901 cgacgcggtc ggaaacgctg atgacggcgg acacttcgtt ttcaggcagt ccgagcctgc
    21961 cccggcaaag cagccattgt gccgtgccgt cggcaaggcc ggtcagcggg gcgggcaggc
    22021 ggacgggttc ggcgtagcgc agatagacgg tggtgatggc gtgatagcga aggttttgat
    22081 atgccgtctg aacgtgttcg ggcgtgcctt cgggcaggag cgcggcggcg tggtagggcg
    22141 cggtggcggg gacggcggca tcgaaagctt cgccgttgac gagcactttc ccgtccggga
    22201 tggtgttcag acggcatacg cgcgtttcga ggcggatgtc cgcgccgagc cgttgaagct
    22261 ccgccaaggc gggttcggcg acgattgcgc ccaaatcctg cttgggtagg agatagtcgc
    22321 tgccggattt tttcgtcagc acgccgtcgg acaaaacgtt gcacaacacg cgcaggcttg
    22381 cggtttccaa aggcgtgttg agcgcgcccc acaccagcgg ctgccaaaac tgcatcacgg
    22441 cggcacgcgg cacgttccgc tgtttcagcc attgtgcaac tgtcgtgtcg ggctgtccga
    22501 ggcgtgcgga cttctgcaaa tcggacatat cggcaagcag tttggctttg aatgcggacg
    22561 gtacgcgccg ggcaagcagc acgccgccca aaatatgcag cggtgcgggc agggggaggg
    22621 cgcggaactg caaaccgccg tgcatatgcc agtgcagcgg tacgcgcaaa aaggcggcac
    22681 ggggatccga accgatggtt ttcatcaggc gcaacacgcc ccggtatgcg ccgagcaaaa
    22741 tgtgctgccc gttgtccaaa aaaccgaaac cgtcggtatt tccggccagt gtgcgcgccc
    22801 tgccgcccgc ctgccggccg gcttcaaaca gggtaacgtc ggcgtgccgc gccaaggtga
    22861 cggcggcgga cagtcctgcc cagcctgcgc cgatgacggc gattttcggg cgcggatgcg
    22921 gcgtgttcat catttattcc tccaatggtt gaaaccccgt ttttcggggc ggcgggggtt
    22981 gggttttcct gaagggcggg cggtatgcgc cttatgcccg ttccggcgtg ccggggcgcg
    23041 gtttgaatcc gaataaccag gttttcaggg caatgcgttt tttgcgcggc gaagggaggg
    23101 cgattttgta tttgaggacg ttttgcgcgc cgtcccggtc gatttcgttc aatagctcgt
    23161 aataaaccgc cgccataacc agtccgactt tttgggcttt tttatcggca gccggcagca
    23221 gcgatacggc ttcgcggtag gtttcacggg cgcgtttgat ttggaacgcc atcaattcgg
    23281 caaaattgcc cgtcgggctg cattgcaaaa tcacgcttgc ggggacatca aaccgctgca
    23341 tttcctccat cggcaggtaa atccgtcccc tgcgcgcgtc ttcgccgaca tcgcggatga
    23401 tgttggtcag ttgcagcgca agtcccatct tgtcggcgta ttccagcgtt ttgccgtctg
    23461 aaaaccccaa aatccgcgca atcaggcagc cgactacacc tgcaacacgg cggcagtaca
    23521 gtttcaattc ttcaaaactg ccgtaacgcg cccgaaccaa atccatctgc atcccgtcga
    23581 ttaaggcttc cagttcatat ttcggcagct tgaaggtttc cttaacctgc cgcaaggcct
    23641 gattgacggg gtgttccggc atcgcgccgc cgaatacctt gtccaaatcg ccgcgccacc
    23701 agttcaatgt cgcctgtgca acatcggggt tggaacattc gtcaaccaca tcgtccaatt
    23761 cgcggcaaaa agcatataaa accgttaccg catcccgttt ttcctgagtc aggaaacgga
    23821 agcccgacaa aaaactggag cggctttctt ctgctttttg gcggcaatag tcgagtcctt
    23881 tcacgattta tattcctaat gatgggcggg aaaggcggat tttatcggca tttggcggta
    23941 gagggcaatt tcggcggcac gacccaatcc ttagcggttt gctcaactat cggcgcgaat
    24001 tctgttaaaa tgccgacttc cttcctttac acaccgcacc gacaggcaga atttatggct
    24061 cttttgcaga tttcagaacc gggtatgtcc gccgccccgc accgacaccg tttggcggca
    24121 ggcatcgatt tgggtacgac caacagcttg gtcgccaccg tccgcagcgg cagtgccgcc
    24181 tgtctgcccg atgccgaagg gcgcgttacc ctgccttccg tcgtccgcta tctggaaaac
    24241 ggcggcattg aagtcggcaa aaccgccctg tccgcccaaa aaaccgaccc gctgaacacc
    24301 gtcagctccg ccaaacgcct tatcgggcgg acgcttgccg atctgcatca aaatacgcat
    24361 tacctgcctt accgtttcgg tgacaatcaa cgcgttatcg aactgcatac gcggcagggg
    24421 gtgaaaacgc ctgtcgaagt gtcggcggaa atcctcaaaa cccttaaatc gcgcgccgaa
    24481 gaaaccttgg gcggcgattt ggtcggcgtg gtgattaccg tccccgccta tttcgacgat
    24541 gcccaacgcc aggccaccaa agatgccgcg cgtctggcgg gtttgaacgt attgcgcctg
    24601 ctcaacgaac ccaccgccgc cgcaatcgcc tacgggctgg acaacgcctc ggaaggcacg
    24661 tttgtcgtgt acgacttagg gggcggcaca ttcgacgtat ccgtattgca actgaccaaa
    24721 ggactgtttg aagtcaaagc caccggcggc aacagcgcgt tgggcggcga cgacttcgac
    24781 catcgtttgt tctgccacct gctcgaacaa aacgagctct ccaaactcaa cgagcaagac
    24841 agccggctgc tgctttccct tgtccgtagt gccaaagaaa aactgaccgc tcaaaccgaa
    24901 gccgtcatcg aaaccacact ttcagacggc cataaagttc ataccgtcat tacccgccaa
    24961 gagtttcaca atctgaccca aaatctggta caaaaaacca tcgagccggt caaacaggct
    25021 ttgaaagatg cgggcgtggg caaggcggat gtcaagggcg tgattatggt cggcggttcg
    25081 acccgtatgc tgcacgtcca acaagcagtt gccacattct ttggtcaaac cccgctgaac
    25141 aacctcaatc ctgacgaagt ggtcgcgctc ggcgcagcca tacaggcaaa cgtcctcgcc
    25201 ggcaacaaag ccgacggcga atggctgctg ctggacgtta ctcccctgtc gctcggtttg
    25261 gaaacctacg gcgggcttgc cgaaaaaatc atcccgcgca attccaccat ccccacctcg
    25321 cgcgcgcagg acttcaccac gttcaaagac ggtcagaccg cgatgacgat acacgtcgta
    25381 caaggcgaac gcgaactggt ttccgactgc cgcagccttg ccaaatttac cttacgcggc
    25441 attccgccga tggcagcagg cgcagcgcgt atccgcgtaa ccttccaagt tgacgctgac
    25501 ggattactat ccgtttccgc acaagaacaa agcaccggcg tacaggcgca aatcgaagtc
    25561 aaaccttcct acggcttgga cgacagcact atcacccaaa tgctcaaaga cagcatgagc
    25621 aacgctgccg aagacatggc cgcacgcgca cgcgccgaag ccgtagtcga agccgaaagc
    25681 ctgaccgatg ccgtcaacgc cgccctcgag ttggacagcg atttgctgga tgccgaagag
    25741 cttgcacaaa tccagcgaga catcgctgat ttgcaaggca gtctgaaaga cggcaacgcc
    25801 gaagacatcc gcgccgccgc cgccaaactc agccgcagca ccgacaactt cgccgctaaa
    25861 cgcatgaacc gcaatatcca acgcgcattg actggtcaaa gcgttgataa tatttgacgg
    25921 caaatggttt cagacggcat tggataaaga ggccgtctga aaaaatatca tctcacagaa
    25981 ataaaataaa aaacaacaat ggaaaagtca ggcattgtct atctgatgaa aaccgtcatc
    26041 aagggcgtgt ataaaatcgg catttcggat gtaagcaatt ttgaaggcag aatgcgccat
    26101 ttggaaaaca acggttatgc gaacgttgcc gggttggaac gcatcctcgc tgtcaaaacc
    26161 gacaattaca aagaaaaaga aaacctgctc catgaaattt tcagcaaaag caggataggc
    26221 gataccgaat tgttcgccgt ggacgaaaac cttgtgaaac gtttgttttt atcgcttcgc
    26281 ggcgaaatcg tgttcccgaa aaacgaaacg gcggaatcgg aatttgaaaa aagcgtccac
    26341 gaacgcaggc aggaagggaa tgccggatcc ggccgcaaac aactgcttga tttggtacgg
    26401 cgcggacacc gggaataccc ttacgcgctg cctcggcttt tggcgggtgc ggcgtcctac
    26461 aagccgaaaa aatcgaaaat ccgccttttt aaagaagcat atttcggcaa aagcggcacg
    26521 aggctgaccg acgaaattgc agacggcatc catatttaca cctgtttttc gcgggcggat
    26581 ttggaaaaag cctattccga atatttggaa tttttcaaat ccgaatcgga tgccgaaggc
    26641 agaaaaccac gctaaggtgc aaacagatac cgtacacgtt gaggagcagt atgatgggcg
    26701 attccgtcat ttattatgta gaacaggcag acgaaccggt aaaccgtgcc ggcgaacgcg
    26761 cccgtaaaac attcaaatac ttttggcgcg aacttttttg ggaacgccgc cgcattattc
    26821 ccgccctgga ttttgccatg gtcaaagtcc cttttttcca agacggcgaa gacggcgaaa
    26881 tttgcgaaca tatgtggatc gacgatattt attttgacgg tctttacatt tacggtgtgc
    26941 tgaacaatga acccggcgaa ctgaccaata tcgaacaagg cgaaagcatt tgcgttccgg
    27001 ttgacgacat cagcgactgg atgttcgtgt gcaacggcat tccctacggc ggctttacca
    27061 tacaggcaat gcgcgggcag atgacggaag aggagcgtac cgaacacgat gccgcatggg
    27121 gaatcgattt cggcgatccc gggcaggtat tgctggtgta tgaagaaaaa gaacatcccg
    27181 aaaatctgga agagcatccg atgtgccgga actgtattga cgattttcgg caacagttgt
    27241 cccaaaatcc ggattttctg catgagcagg acgaagacgg ctatacgccg cttcatcatg
    27301 aagccatcgc aggaaatgca cttatggttc aagccatgct tgaatacggc gcaaatcctg
    27361 cctcaaagac atcggaaggc tataccgccc tcgattttgc ccgcctgacg ggctggcaaa
    27421 atgttgccga cctgctcgaa ccgcgacatt aggcagacag ttttccgaaa acgaacacaa
    27481 acacttttta cagaaagaca ataaaaatgc ccaaaatcac cgtacttcca cacacgacat
    27541 tatgccccga aggcgcggtt atcgacgacg cgccggaagg caaaaccgtc ctcgacgtac
    27601 tgctcgacca tgatatcgaa gtcgatcacg cctgcgaaaa atcctgcgcc tgcaccacct
    27661 gccacgtcat catccgcaaa ggtttcgaca gtttggaaga accgaccgaa ttggaagaag
    27721 acctgctcga ccaagcatgg ggtttggaag ccgattcgcg cctgagttgt caggcggttg
    27781 tcgccggcga ggatttgatt gtggaaatcc ccaaatacac catcaaccac gcgcgagaag
    27841 aacactgaaa acaggccgtc tgaagccggc gcgcttcaga cggcattgtt gcgcggataa
    27901 ggcgcaatta ccggaaacag gcgttcgtac aggcggaaat ttcgattcta tattgatttt
    27961 tatcggtttt ctgacggaat gatccagtgc ggcatccgag gcggattact cgggcgcgat
    28021 gcacgggtat ttatcggttt tgcagccgga aaaaccgccg gcgggttact ttcagggcga
    28081 cggtcgggca gtatgccgga tagcgttcca ctcttgcttc tatattgatt ttgattggtt
    28141 ttctgacgaa acggccagat gcggcatccg ggacggcttg tgttttttcc tgcccgcctg
    28201 ccggattttc ccatccttgc gtgaaaccga aagagacggc ggcgcggcgg acaagctcga
    28261 gatagcgtcc ttcaagctcc gggcaggcgg cggacacgct ttctgccgta accgtgaagc
    28321 cgccgtccga cccggcaagg cgttccgcaa tctgcgtgta gatgcgccaa cgttgggcgc
    28381 gcaacattgc cgcatcgcaa ccggcagccg ctttatccaa ggcaatcagg ccgcgcctgc
    28441 tccggtagtg aaggcaaacc gttactctgg agagggtatg ttgcccgtcc aatatttgat
    28501 acagcttgcg tatcagcaaa atcaggcggt caaactcccc catctccgac agggggtcgg
    28561 gatggtcgga agcggtataa accagtttgg acaattttgc ggcaaacata ttgttcatca
    28621 atcttccttg tcggttgaaa ccccgctctt cagggcggta gaatcagatt tgtttgggag
    28681 aggcagctcc tcccgaatca ggacgacaca taaggtggtg cttgatgtgt tgtccggcga
    28741 gttgaaacat tcagccatcc tcaaagggcg gcagttttgc cgaaacatat tctacacggc
    28801 ttcaatgccg gacgataaaa ggaaattcat atgaaatgga cagacacaca acgtatcgct
    28861 gaagaccttt acgaccttca cggcgaagat atcgacccca aaagcctgcg ctttacccaa
    28921 ttgcgggatt tgattatggc gcttcccgaa ttcgacgacg accccgcccg ttgcggcgaa
    28981 cgcatcctcg aagccgtgca gcaggcatgg atagacgagg cggaataagt ttcgggaatg
    29041 ccgtctgaaa tgcggcggta cgcggttcgt gcttctgttt gcagcgggaa tggttttacc
    29101 agtctccttt tttcagcctg tccagttggc ggcggtcgcg cttggtcggt ctgccgtcgg
    29161 gataggcgga agtgatgcgg ctgaattggt cgagctgttt gcgctcttcc ctcaatgttg
    29221 ctgttttcgt gtcttcttca tacagaagtc gcgcctcgga tgccgggcgg cgttggtggt
    29281 tcaaaccttt aaccttgatt ttatagggaa gggaattgag cgtcaggtcg ataatatcgc
    29341 cgatgtctat ggttttactg tttttgacct tcgagccgtt tacttgaacc ctacccagtt
    29401 cgatgtgctt ttgcgcaagg gaacgggtct tgaaaaaacg tgccgcccaa agccatttgt
    29461 ccagccgcat ggcggaagaa tggtgcttgt ctttcatacg attttgtttg aaataattga
    29521 atttgtttcg agtttagcat aagatacgcc gcctataact agtatatatg cactaatcca
    29581 ctgttttcca tgctgtccga acacaaaaag agggtgtgga aaagccgttt tggacaataa
    29641 attaactgcg gaatatgcac aaatagcgta tgatagcggc agaatctgtt gatgagagct
    29701 tcattctatg aaacctgttt ttttggattt tgaacaaccc atagccgaac tgaccaacaa
    29761 aatcgatgag ctgcgtttcg tccaagacga gtctgccgtc gatatttcgg acgaaataca
    29821 ccgtttgcag aaaaaaagca acgacctgac caaatcgatt ttcagcaaac ttacgcccgc
    29881 ccaggtttca caggtttccc ggcatccgca gcgtccctat actttggatt acattgatgc
    29941 gctgtttact gattttgaag agctgcacgg cgaccgccac tttgccgacg atcatgcgat
    30001 tgtcggcgga ttggcgcgtt tcaacggaca aagcgtgatg gtcgtcgggc atcagaaagg
    30061 gcgcgacacc aaagaaaaaa tccgccgcaa cttcggtatg ccgcgccccg agggctaccg
    30121 caaagccctg cgcctgatga aaacggcaga aaaattcggc ttgcccgtca tgacctttat
    30181 cgatacaccg ggcgcgtatc ccggcatcgg cgcggaagaa cgcgggcagt cggaagccat
    30241 cggcaaaaac ctgtacgaac tgacacgcct gcgcgttcct gttttgtgta ccgtcatcgg
    30301 cgaaggcggt tcgggcggtg cgttggcggt cgccgtgggc gattacgtca atatgctgca
    30361 atactcgacc tattccgtta tttcccccga aggctgcgct tcgattttgt ggaaaaccgc
    30421 cgaaaaggcg gcggatgcgg ctcaggcttt gggcattact gctgaccgcc tgcaaaagct
    30481 ggacttggtc gataccgtca tcaaagaacc attgggcggc gcgcatcggg attttgggca
    30541 aaccatgaaa aacgtgaaag ccgttttgga aaaacaactg cacgaagcgc aaagcatccc
    30601 gcttgccgat ttgctttcgc gccgtttcga ccgcattatg gcttacggca aattttcgga
    30661 acaataattc aggtagaaca agcagcaagc agtttgtctg aaactgcttg ctttttcttt
    30721 atcgggacgg aaccgtgctg actttagatg cgtttgagca atgcttgaag gattgttttc
    30781 ctcaaggtct gaatggaaaa aaaacagcgg tggcattaag cggcggcttg gattccgtcg
    30841 ttttgctgca tctgcttgtc cgcgccggga aaaaaggcgg ttttattccg gatgcattgc
    30901 atatccatca cggcttgagt ccccgtgccg acgattgggc ggatttctgc caaaactatt
    30961 gcgatatgct cggggtgggg ctggaaacgg ttaagatctg cgtggaaaaa aacggtttgg
    31021 gcatcgaggc ggcggcgcgg caaaagcgtt atgccgcgtt tgccgaaaaa ggctttgacg
    31081 ttttggcgtt ggcgcaccac agggacgatc aaatcgaaac ctttatgctg gcggtcgcgc
    31141 gcggcggcgg tttgcgcgct ttggcggcta tgcccgccgt ccgccctttt ggggaaaaag
    31201 gcattatctg gcggcccttg ctgccttttt cgcgccaaga tatatgggat tatgcccaaa
    31261 aacacggttt gccgaatatc gaggacgaaa gcaatagcga tacggcttat ttgcgaaacc
    31321 gcttccggca ccgtattttg cccgaacttt cggcgcagat tccccatttc gggcggcatc
    31381 tgttgaacaa tgtccgtgcc ttgcaggagg atttggcttt gttggacgag gtcgtcgttc
    31441 aagactgccg ttgggtttgc ggagccggtt atttcgatac ggcgcggtgg ctgacgtttt
    31501 ccccgcgccg gaaaacccat attttgcggc attttttgaa ggaaaacggc attcccgtgc
    31561 cgaatcagaa tgcccttgcc gacattgccc gggttttgac ggaggcaaaa accggacgtt
    31621 ggaacgtgca aggctttgaa ttgcatcatt atgcaggcag gctgtttgtg ttccgaccgg
    31681 aaaaaacgga taaactgcgg tttttgaaag acaggcagat aagcggaaat ttaagggaaa
    31741 tattgacggg gcagggattt gtgttgaagc ggcatccgtt tgggcttcct gagcatcttt
    31801 tggagcagga cggaattttg aggacggtag cggcatcgga tacgttggcc gtgggcggca
    31861 tccataaaaa tgtgaaaaaa atccttcagg aaaagcgagt tttgcccacc ctgcgcccaa
    31921 tctggccgct cgttgccgac agcggaaaca gtccattggc gttggcaaac tgttgtgcgg
    31981 atttccaata ttcggtttca gacggcattt tgcccgtcca tcctgacttt cccattttat
    32041 tttgataata tcgcaaacag attttggcgg cgttcagtcg ggtattgtcc ggttgcatat
    32101 ttctaaaagg cttgtgaagt gaaacacatc agttcgacca ataatgaaca catcagacac
    32161 ctgcaccgcc tgttgtcgca aggaaagttc agacggcagt atgcccaaac cgttttggag
    32221 ggcgtgcacc tgcttcaggt tttcctgcaa tccggcggaa agccgctcgg ggtatatatt
    32281 cccgagggca aaatgccgtc tgaagaaatc cgcaggctga gggcggttgt gccggaaggc
    32341 aggatttttt ccgtttcaga cggcatattg aaaaaaatca gcagcttgag ttgtgcagac
    32401 gatatacttg cactgattga tatcccattg ggcggaacct tgccggacaa aggcgactgt
    32461 gtggttttgg acggcgtgca agatccgggc aatgtcggca ctgtcttacg gagtgcggcg
    32521 gcggcagggg taggtacggt cgttttggac aggggttgcg cggacgcgtg gtcgcccaag
    32581 gtattgcgcg cggggatggg cgcacatttc ttgtcgggca tttatccgca ggcggatttg
    32641 gaaatatggt tggcgcgcta taaaggccgt gtgtttgcca ccgccttgcg cgaggaaaag
    32701 caggcggttt tgtacggcga agatttgtgc gaaccgacag cctgggtgtt tggcaacgaa
    32761 ggcgcggggg tcggtaaagc agttttagac agggcggaca agtgtgtcag gataccgatg
    32821 cacgatgcaa ccgagtcttt aaatgtcgcg atggcggcga caatctgcct gtttgaacaa
    32881 atgcgccaac gggcggcgta ttgaggaaaa gaaatgccgt ctgaaaaaat ctattacggc
    32941 gtattgattt tcttatgtat cgcttctatg ctgctgtcgc cgttttttta tgcgggtgct
    33001 ttgaagccca agaaggcggc attgcggaag gacgagcagt ggaaactcat cttattgtcc
    33061 aatgccgtgg cggcggcggt tttggcttgg gtgtggtgga aatggttttg aaggagtctt
    33121 gatatgaaaa catctaaaat tattgcgggg ctgatgctgg ctgtggcggt tcagacggca
    33181 tctgcggcag tgtatgaatg ccgtcaaaac ggtaaggtca gttacagcca aactccggga
    33241 aaacattgta ccaacgcggg tttggggcgg gatcgggtgt acagttcggt tagaccggca
    33301 gtaaaagaca gggcggaaga cgcaggggtc ggcgattatt cggacacggt gagggacgaa
    33361 cacgtccaaa atccgagaga gaatacgcat aaggatgctt cgaataccgg tgcaaagacg
    33421 cattgattga aaccgaatca gccatgacgg cgttgaataa tagaatttga acgattaggg
    33481 aactctgatg aaacacatcc acattatcgg tatcggcggc acgtttatgg gtgggattgc
    33541 cgccattgcc aaagaagcag ggtttgaagt cagcggttgc gacgcgaaga tgtatccgcc
    33601 gatgagcacc cagctcgaag ccttgggcat agacgtgtat gaaggcttcg atgccgctca
    33661 gttggacgaa tttaaagccg acgtttacgt tatcggcaat gtcgccaagc gcgggatgga
    33721 tgtggttgaa gcgattttga acctcggcct gccttatatt tccggcccgc aatggctggc
    33781 tgaaaacgtg ctgcaccatc attgggtact cggtgtggcg gggacgcacg gcaaaacgac
    33841 catcgcctcc atgctcgcat gggtcttgga atatgccggc ctcgcgccgg gcttccttat
    33901 cggcggcgta ccggaaaatt tcggcgtttc cgcccgcctg ccgcaaacgc cgcgtcaaga
    33961 cccgaacagc caatcgccgt ttttcgttat cgaagccgac gaatacgaca ccgcgttttt
    34021 tgacaaacgc tccaaattcg tgcattaccg tccgcgtacc gccgtgttga acaatctaga
    34081 attcgaccac gccgacatct ttgccgattt gggcgcgata cagacccagt tccaccacct
    34141 cgtgcgcacc gtgccgtctg aaggcctcat cgtctgcaac ggacaacaac aaagcctgca
    34201 agacactttg gacaaaggct gctggacgcc ggtggaaaaa ttcggcacgg aacacggctg
    34261 gcaggccggc gaagccaatg ccgatggctc gttcgacgtg ttgcttgacg gcaaaaaagc
    34321 cggacacgtc gcttggagtt tgatgggcgg acacaaccgc atgaacgcgc tcgccgtcat
    34381 cgccgccgcg cgtcatgccg gagtcgacat tcagacggcc tgcgaagcct tgagcacgtt
    34441 taaaaacgtc aaacgccgca tggaaatcaa aggcacggta aacggcatca ctgtttacga
    34501 cgacttcgcc caccatccga ccgccatcga aaccaccata gaaggtctgc gtcagcgcgt
    34561 cggaaacgcc cgcatcctcg ccgtcctcga gccgcgttcc aacacgatga agctgggcac
    34621 gatgaagtcc gccctgcccg aaagcctcaa agaagccgac caagtgttct gctacgccgg
    34681 cggtgtggac tggaacgtcg ccgaagccct cgcacctttg ggcggcaggc tgcacgtcgg
    34741 caaggatttc gatgccttcg ttgccgaaat tgtgaaaaac gcccgaaccg gcgaccatat
    34801 tttggtgatg agcaacggcg gtttcggcgg aatacacacc aaactgctgg acgctttgag
    34861 atagcccggg cgatgccgtc tgaaagccct tcagacggca tcgtccggct gcgcggcaca
    34921 aaggcggaaa aaccgtttgc cccgtatttt caaacgcgtt acacttgccg ccgctgtttt
    34981 cagccatttg attacccgca accgccgtca ttgcgccggc ggtttgcctg tcagcgtcat
    35041 tgcgccgctg taaatacgaa agaacacatt atgaccgtat cccccgtcgc cttgcgccgt
    35101 aagaccgagt gcaagcctca tcccaccgcg cgctattgga aaaaatgcga tgtcgaagcc
    35161 ctgttcggac ttcccttcct cgacctcatt taccaagccg ccgaagtcca ccgccaaaat
    35221 ttcaacccgc gcgaaatcca gctttccacg ctgttgtcca tcaaaaccgg cggttgtccc
    35281 gaagattgcg cctactgtcc gcaatcggcg caccacaata ccaatctggg caaagagcag
    35341 atgatggatg tggatgaaat cgtcgaaaaa gccaaaatcg ccaaatcgcg cggcgcaagc
    35401 cggttttgta tgggcgcggc atggcgcggc cccaaaccca aagacgtgga gacggtttcc
    35461 gcaatcatca aagccgtcaa gggcttgggt atggaaacct gcggcacgtt cggtatgctc
    35521 gaagaaggta tggcggaaga cttgaaagag gcgggcttgg attattacaa ccacaacctc
    35581 gacaccgacc ccgaccgcta caacgacatc atccatacac gccggcatga agaccgtatg
    35641 gacaccttgg gcaaagtccg caacgccggt ttgaaagtct gctgcggcgg catcgtcggg
    35701 atgaacgaaa cccgcgccga acgcgcaggg ctgattgcca gcctcgccaa cctcgacccg
    35761 cagcccgaaa gcgtgccgat taaccggttg gtcaaagtgg aaggtacgcc gcttgccgat
    35821 gccgaggatt tggactggac ggaatttgtc cgcaccatcg ccgtggcgcg gattacgatg
    35881 ccgcaaagtt atgtccgact gtcggcaggg cgcagcaata tgccggaagc gatgcaggcg
    35941 atgtgcttta tggcgggcgc gaactcgatt ttttacggcg acaagctgct gaccacgggc
    36001 aatccggatg aagacggcga cagaatcctg atggaaaagc tcaacctgta tcccttacag
    36061 tttgagctgg aaggcgaggt cgccgaagtg gaaaaagcct ctgggattaa agtggattat
    36121 tgacgattgc aaaaatgccg tctgaaaccc gaaaaaaggc tttcagacga catttgttcg
    36181 gacggtattt ccaatatctt tttaccggcg cgtgatgttg ccgtcgggcg agacatccag
    36241 cccgttcccg ttggggaagt cgctgcggtt cagataaata atccgcccga taacggtttt
    36301 tcagggtcga ttttggggat tttatcgtcg gcttgggtaa tagggatgat agttccggaa
    36361 ataaatttac cctgtttgtc ggtgataatt ttgaaaatcg gcgcaatgcc gctgatgccg
    36421 gaggtgttga ttgcgccgta ggtggcaaag ttgccgccgc tgtaagagat gaagcggtcg
    36481 cggtaaagtt cgacggcgcg tgtaacgtgc ggcccctgcc cgaatacgac atccgcgccg
    36541 gaatcgacgg caagccgtgc aaactcgacg acgttgcccc tgttttcccc atagaagatt
    36601 tcggtatcga acggcaggtg ttccgcctgt ttcccttccg cgccgccgtg gaacattaca
    36661 atgacgatgt cggctttctg tttggttttg gtaatccgtt ttctaacttt ggcataatcg
    36721 ttcagtttga cggcggcaag gttgggggcg aaggagacga agccggatct tacgccgttt
    36781 ttcttcagga tggcggtttc aaacctgttt tcgatgcccg aatatttgat gttcaattcg
    36841 tcaaggttcg ccctttatgc cgtttccgca ccgcaaaccg ccggggtcaa gccctcggcg
    36901 catcctgccg gaacggaatc cccgtgctgc cgattgacgg ttcaaacccc gcgcccgttt
    36961 caaataccgg cgatgtggac ggacaggatg cgcctgacga aaagacagcc gataccgttt
    37021 ccattatcgg cgtgggcgac attatgctcg gcagcaatta tccggtcgat tacctgcccg
    37081 ataccaatat tctgaaaaac gtcgaatctg ccttgcaaga cgcggacatt accgtcggca
    37141 acctcgaagg cacgctgttt gacgaaggcg gtacgccgaa aaaatgtgca accccccaaa
    37201 atatgctatg cattccgaac gccctccgca tacgggcaat accttgccga cgcgggattc
    37261 gactacctca gcttcgccaa caaccacagc aacgacttcg gcgcgcaagg catcacggca
    37321 acggcggcgg gcggcagctc ttttacatac tcgatcgcgc taaagccgct gccgataacg
    37381 aggccaaaat tgcggaaaat accgccatcg cccagataaa tttgtccatc atcagacctt
    37441 tactgttcag acgagacagc atttgccgca cgttttgggg cttatctttc gatttgcgct
    37501 acgtcgcgca ccgcgccttt gtcggcggaa gtcgccatcg cgccgtaagc acgcagggct
    37561 gcggagacgt agcggtcgcg gttttccggt ttccacgctt tgctgccgcg cgcttccatt
    37621 tcggcacggc gggcggcaag ctcttcatcg gaaatggcaa ggtggatgct gcggttgggg
    37681 atgtcgattt cgacggtatc gccttcgtgt accaaaccga tcgcgccacc ttccgccgct
    37741 tcgggcgagg cgtgtccgat ggacaaacct gatgtgccgc cggagaagcg tccgtcggtt
    37801 aagagagcgc aggctttgcc gaggccttta gatttcaggt aggaagtcgg atacagcatt
    37861 tcctgcatgc ccgggccgcc tttcgggcct tcgtagcgga tgatgacgat gtcgccggcg
    37921 acgatttggt tgcccaaaat gccttctact gcgtcttctt ggctttcaaa cacgcgggcg
    37981 cggccggtga atttgaggat gctctcgtcc acgcctgcgg tttttaccac gcagccgcgc
    38041 tcggcgatgt tgccgaacaa gaccgccaaa ccgccgtctt gcgagtaggc gtgtgccacg
    38101 tcgcggatac agcctttttc gcggtcgagg tcgagggttt tccacatacg gttttgcgag
    38161 aacgcttggg tggtgcgtac gccgccgggc gcggctttga agcgttcgat ggcgcgggtg
    38221 ttttcagggt tggttatgtc ccatttttca atcgcgtctt tcagcgtcgg tgcgtggata
    38281 gtgtgcacgt cggtgtgcag cttgcccgct ttgtccaatt ctttcaggat ggcgaaaatg
    38341 ccgccggcgc ggtgcacgtc ttccatatag tagtcgtggt tgttgggtgc ggttttgcag
    38401 atgcagggca caacgcggct taagcggtca atgtcggcca ttttgaaatc gacaccggct
    38461 tcgttggcaa cggccaacag gtgcaaaatg gtattggtgc tgccgcccat cgcaatatcc
    38521 atcgtcatag cgttttcaaa cgcttttttg gtggcaatgc tgcgcggtaa cacggtttca
    38581 tcgttttgct cgtaatagcg tttggtgatt tcgacaatca tacggccggc ttcgaggaac
    38641 agttctttgc ggccggcgtg ggtcgccaaa tacgaaccgt tgccgggtag ggacagaccg
    38701 agtgcttcgg tcaggcagtt catcgagttt gccgtaaaca tacccgaaca cgagccgcag
    38761 gtcgggcagg cgttttgttc gacttcttca acttgccggt tgctgacatt gtcgtccgcc
    38821 gattcaatca tcgcgtcaat caagtccaaa cggcgttcgg gctggatgtt tgccacgccg
    38881 ataactttac ctgcttccat cggaccgccg gaaacgaaga tggtcgggat attcagacgc
    38941 atcgcagcaa tcagcatgcc cggggtgatt ttgtcgcagt tggaaatgca caccagcgcg
    39001 tcggcgcagt gggcattgac catatattcg atggagtcgg caatcaaatc gcggctgggc
    39061 agggaataca gcataccgct gtgtcccata gcgatgccgt cgtcgatggc gatggtgttg
    39121 aattctttgg cgatggcgcc cgccttttcg atttcgcggg caaccagctg gcccatattg
    39181 tgcaggtgga catggccggg cacgaattgg gtgaaggagt tggcaacggc gatgatgggc
    39241 ttgccgaagt cggtttccat cacgccggtg gcgcgccaca atgcacgtgc gcccgccata
    39301 ttgcggccgt gggtggaggt tttggagcgg tattctggca tagtgtgttt ccttgtctgc
    39361 tatgccgtct gaacggcaaa aggctttcag acggcatttt tgttgtgttt tgaaatgaaa
    39421 aaaaaggtat tttataccaa gtatcggaat tttgcggaat ggaaacgcgt gcggcaaaaa
    39481 aggacatccc ccgcaggaat gcgggggacg gtttcaggcg cgcgcaatcg ggacggcttt
    39541 ggatgcgtcc caaaaatcga cgggcgcatc caacacgggt ttgacgatgc ccgtccgtat
    39601 ggcgaaatcg ccgaaacctt cgtcggtatc gcgtcccgcc gcccatttgc cgatcaggtc
    39661 gtctaattcg gaaaggattt ccggcagggt aatgttttct ttgtagagtc gggggatgcg
    39721 tacgccttcg cggtcgccgc cgatgtggag gttgtagcgt ccgacggctt tgccgaccag
    39781 tccgatttcc gccaacatcg cccgtccgca gccgttcggg cagccggtaa tgcgggtaac
    39841 gatgtagtcg tccgacgtgc cgtgtttcgc cataatctta tccagctcgc cgatgaagtc
    39901 cggcagcacg cgttcggctt ccgccattgc cagcgggcag gtcgggaagg aaacgcacga
    39961 catcgcattt tcacgcagtt tactgacatc gttgcggatt aatccgtatg ttcgggcaag
    40021 ttcttcgatt tttgctttgt ctgcttcggc gacatttgcc acgatgaggt tttggttggc
    40081 ggtgatgcgg aaatcgcctt tgtggatttt ggcgatttcc aacacgccgg tcagcagctg
    40141 tttcccgcct tcgtcaacca aacgcccgct ttcgatgaaa agggtcaaat gccagttgcc
    40201 gtctatgcct ttcacccagc cgatacggtc gccgcgcccg gtaaatttga acgggcgtat
    40261 gggttcgaac ggcataccca tacggcgttc gacttccgcg cggaagttgt ccaagcccat
    40321 attttgaatg gtgtagcggg tgcgggcgtt tttgcggtcg ctgcggttgc cgaagtcgcg
    40381 ctgcgtggtt accaccgctt cggcggcctt cagcgcgtgt tccggaggca cgaaacccag
    40441 ttccagtgaa atgttcggat aggttttggt gttgccgtgt tccatcgaaa gcccgccgcc
    40501 tgccaaaaca ttgaagccgg caagctgtcc gttaccgtct gaaacggcga cgaaatccaa
    40561 atcgttgccg tagcagtcca catcgttcaa gggcgggatg acgactgcgg ttttgaattt
    40621 gcgcggcaga taggttttgc ccaaaatcgg ctcgtcttct tgaaggaagt cgtcggaact
    40681 ttgaactttt ttgccgtcca cccacacatc cagataaccg cgcgtgcgcg gcagcaagtg
    40741 ttcggaaatc tttttcgcgt attcgtaagc ctgccggtgc agttcggact cgatcgggtt
    40801 ggacgtgcaa agcacgttgc ggttcatatc cgccgccgtg gcgatggaat ccaaacccag
    40861 tttgtgcaag aggcggtgca tcgtctgcaa cttggctttc ggcacgccgt gaaattggaa
    40921 ggtttgccgg ttggtcagcc ggatggagcg gtaatgactg ttttcccggg caaatttgtc
    40981 cagttctatc cattgggacg gtttgatgat cccgcccggc agccggcagc gcaaaagcat
    41041 aaatttcaag ggctcgagtt ttgcctcggc gcgttcggcg cggatgtcgc ggtcgtcctg
    41101 ctcatacata ccgtggaagc ggatgagttg gaagttgtcg cctttgaagc cgcccgtgag
    41161 cgggtctttc aaatcgtcca aaatcgtgcc gcgtaaaaaa ttgctttcgg ttttcagacg
    41221 ttcgttgtcg gatagcggtt tttcttgcca cgccaaacct tttgtcttgg tctgtacggt
    41281 cattttgtgt tcctcccgat tatatttaat caataaacat cacgctgata gcgtttttct
    41341 tcgcgcagca tatccaaata ttcttctgcg ccctcttcgt ccaaatgtcc tgccccgata
    41401 atcacatcca gcaaggcggc ttccacgtct tttgccattt ttgccgcatc gccgcacaca
    41461 tagatatgcg cgccttcctg cagccattgc caaagtcctt ccgcctgttc gcggattttg
    41521 tcctgcacat agattttttc ttcctgatcg cgggaccagg cgaaatcgta cctgtgcagg
    41581 aaaccgtctt tggcaaactg ctgccattcg gtttgataga gaaaatcacg ggcaaaatgc
    41641 ggattgccga aaatcagcca gtttttgcct tccgcatttt ctgcggcacg ttgttggacg
    41701 aaggcgcgga acggtgcgac gccggtgccc gagccgatca tcacaatcgg cttgcggctg
    41761 tcttcgggca gcctgaagcc gtcgttgcgt tccacaaaca cgcgcaccgt gccgtcctct
    41821 tccagccggt cggcaaggaa acccgatgcg ccgcccgccc tggcgcgccc ttcgtgttcg
    41881 aaacgcaccg cgccgacggt caggtgcact tcgtccccca cttccgcctg cgacgaggaa
    41941 atcgaataca ggcgcggcgc aagcgggcga agcaggccgg cgaattgttc cgccgtcagt
    42001 tttgccggga agcggtgcag cacatcggca atcggcgtgc tttgcacaaa gccttgcaaa
    42061 acggcgttgt cggcagcaat acggtcgagt tcgtcatcat cggcgaacgg ggcatagcct
    42121 ttgacaaagg cgggggtgtt ttgcgtgagt tcaaaatggg ataacagtgc ggaggcaacc
    42181 ggcagggttt ttccgcccgc ctgtatttcc gttgccggat cgatgccgag caggtctagg
    42241 atttccctga ccagtgccgg atcgttgtca aaccaaacgc cgagcgcgtc gcccgggagg
    42301 tagtgcaaat ccgaaccgct caaatcgatt tcgatgtggc gcacgtcttt atcggattgg
    42361 cgggaggtga ttttctgatt ggccagcagg gcggcgggaa aggggtctgc cttgcagtac
    42421 ctgccatccg gtgccgtctg aaggccggcg gggggcgttg tctgcggcgc gggcgttgcc
    42481 cggttttttg cggcttcttc ttttaagcgc gcgacaatgc gacctgtcca tccgtctgcg
    42541 gaggcggtaa agtccaaatc cgcatcaacg cgttcgagca ggcgtttcgc ccccaattct
    42601 tcaaaacgcc ggtcgaaatc tttacctgcc tgacagaaat tcggatagga gctgtcgccc
    42661 aagcccagta cggcaaattg gagtttgtcc aatttcgggg cttttttgcc gttcagcagt
    42721 ttgtgcagca cgacggcttc ttccggcggt tcgccttcgc cctgggtgga cgtaaccagc
    42781 agcaggcggc gttcgtcggc gatgtttttc gccttatagt ctttcagttc ggtgcgactg
    42841 acttggatgc cggcggcttc caggctgtcc gccgctttgt cggcaacgga tttcgcattg
    42901 ccggtttgcg aggcggaaag gacggttacg gaaaaaggtt ctgccgccgg caatgccgtc
    42961 tgaagcgcgg gcagtcctgc agatgccccg tttcctgcag atgccccgtt tcctgcagat
    43021 gccccgtttc ctgcttttgc ccaagcgtag ccggacagcc acgcccattg tgccgcgtcc
    43081 agccccgaca ggagctgcgt gatttcgggc ggcagaggcg gtaatggcgg atttgtgttc
    43141 tgcatatcgt gttcactcat aaaatcatac ctgccgcaac agtgccgtat gtcgcttcgt
    43201 ctatcaggat aaacgaaccg gcggcggtgt tttccgcata aggcgttgcc gtaacgggtt
    43261 tttgaaggtt gatgcggact ttggcgatgt cgttcatctt caaggattcc gcgccggcct
    43321 cttgttccag cgtgcggaca tccaaaacgc tttcaatttc cccgactttt gccggcacgg
    43381 tttgcgtgcc gtgcttgagc aggtatctgc gcgcggtgtt gagcggacgt tcgtcaaacc
    43441 agcaaagcgt ggcttccaga tgtttttgcg gggcgagcgg ggaattttta tcgacaaaaa
    43501 ggtcgccgcg cgaaacatcg atgtcgcggt ccagccggag ggttgccgcc tcgccggcaa
    43561 aagcctgcgc cacttcccct ttcggcgtga tgatttcgga cacttcggcg gtcagcccgt
    43621 tcggttcgat gcggacggtt tgcccgacgg cgaccgaacc gcgttcgatg cgcccctgat
    43681 agcctcggaa atcatcggcc ttgtcggcat cttggcggac gaccagttgg acggggaaat
    43741 aaaaatcgtc ggcggtgcgg ctgacttcgt ccgcccccgg caggttttcc aagatggcca
    43801 gcagcggttc gccccgatac cacggcatat tgccgccggg gtaaacgagg ttgtcgccca
    43861 agagtgcgga caccggtacg aaatgcacgt ctttcaaacc gagccgtccg gcgagccggc
    43921 ggtatgcctc cacaatggcg ttgaatttgt cttcgctgta atccagcagg tccattttgt
    43981 tgaccgccac cacaatatgc gggcagttga gttggcggag gatggcggaa tggcgtttgg
    44041 tctgcggcag aagctgcaag ggctgcgcgc cgaaatccag ttgggatgcg tcaaccagca
    44101 cgactgccgc cgaagcggtg cttgcgcccg taaccatatt gcgcgtgtat tgttcgtgcc
    44161 ccggcgtgtc ggcgatgatg aatttccgtt tcgccgtgga aaaatagcgg tatgccacat
    44221 cgatcgtaat gccctgttcg cgttcggctt ccagtccgtc ggtcaggatg gagaagtcta
    44281 tggcttcttt caaacctttg cttttgccgg attccaaggt tttgatttgg tcggacagca
    44341 gggctttgct gtcgtagagc agtcgtccga tcagggtgct tttgccgtca tcgacgctgc
    44401 cggcggtaat gaagcggagc ggggcctggt gttgtgccgt catattttct tcctcatatc
    44461 tgcttaaagg gtttttgaaa tttagaaata gccttctttt ttgcgttttt ccattgccgc
    44521 ctcgcttgcc tgatcgtcta gccgggtcgc gctgcgttcg gaaatgtcgg caaccgccgt
    44581 ttccctgata atctccgccg gcgtggacgc ggtgctttct accgggcagg tgcagctgat
    44641 gtcgccgacg gtgcggaagc ggacatcaag gatttcggag gtttcagacg gcattttcgg
    44701 ggtgagcggc gttacaggga ccagcagccc cctgcgtctg accacttcgc gcctgtggct
    44761 gtaataaatc ggcggcagct cgaggttttc gcgggcgatg tattgccaga tgtcgagttc
    44821 cgtccagttg gaaatcggga agacgcgcat attttcgcct ttgtgcagcc tggtgttgta
    44881 cagcgaccac agctcggggc gttgcgcctt cggatcccat tgtccgaact cgtcgcggaa
    44941 cgagaaaatc cgttctttgg cgcgggcttt ttcttcgtcg cgccgcgcgc cgcccataag
    45001 cgcgtcgaag ccgtttgcct cgatggtttc caacaaggta accgcctgtg ccgcattgcg
    45061 cgaatcggtt tctttgcgta agaccaccgt gcctttggca atggagtctt ccacgcgccc
    45121 cactatcagg cgggcattga gttttgccgc ctgcgcgtcg cggaaagcaa tcacttcggg
    45181 gtagttgtgt cccgtgtcga tatgcaccag cgggaagggc agtttcaccg gccggctgcc
    45241 cagccggaag gctttgcagg cgagggcgag caggaccacg gaatctttgc cgccggaaaa
    45301 gagcagggcg gggttttcac attccgccgc cacttcgcgg atgatgtgga tggattcgga
    45361 ttccaaccag tcgagttggg cgttgttcgg ttcggttttc gtcataccat attccttatt
    45421 tcttctgtct gatatttatg aattatttgt gcagcccgca ttctttgctg tttctgcctt
    45481 cccaccacca ccgcccggcg cggatgtctt cgcccgcctt gacggggcgg gtgcaggggt
    45541 cgcagcctat gctgggaaat ccttgccggt acaaatcgtt gtaaggcaca ttgttggcga
    45601 ggatgtatgc ccacacgtcg tgttccgacc agtcgaaaat cgggttgtat ttgccgatgc
    45661 cccgtccggc atcgtattcg gcaaacggca gttccgtgcg tgtggcggat tgttcgcggc
    45721 gttgcccggt aagccaggcg tccgcgcctg caatggcgcg gttgagcggt tcgatttttc
    45781 ggatgcggca gcattcgcgg cgcgcttcaa cgctgtcgta aaaggcaaac ctgcctttgc
    45841 tttccacata acggtcggca tcttctcgaa ccggccggaa acgctttatc cgcaaatggg
    45901 gatatgcgcg tccgagcctg tccagcaggt ttagggtttc cgtgtggagc agccccgtat
    45961 ccaaggtaaa aatgccgata ttgaggtttt cgccggcgat aaggtcggta atcaccatat
    46021 cttctgccgc aaggctgctg gcaaaccgtg catcccggtg gctgccgaca atccggtgca
    46081 ggcgttgttt gagggtttcc gttttttccg caagggcggt ttcgccgccg gatccgatat
    46141 gcggtatctg ccacagggcg ggtttgaaca gtgtcgtttc catttttccc gccttatgcc
    46201 gcccgttgtc cggcattcag tccgcccaat gcgggatacg cctgcccgac ccggttttct
    46261 ccttcgccgt tttcaccgaa ccaggcgagt ttttcgtgca gccccaccac ttcgcctatg
    46321 acaatcaatg ccggattcgg cgcggtttcg gcgagttcgg caaggttggc gagcgtgccg
    46381 gttgcggttt tttgagccgg cagcgtgcct tggctgatga cggctgccgg cgtgtcgggc
    46441 gagcgtccgt gctgttgcag ccgttcggca atcagggcgg ctttgagcgc acccatataa
    46501 atcaccaagg tctggcggct gcgggcgagg gtctgccatt cgatgtcggg cgcatccgcc
    46561 ttgcggtggc cggttacgaa aaccgcactt tgggcataat cgcggtgcgt gagcgggata
    46621 ccggcatagg cggtcgcgcc gacggcggcg gtaatgccgg ggacgaccga aaacggaatc
    46681 tgatggcgtg ccaaggtttc caattcttcg ccgccgcgtc cgaacacgaa agggtctccg
    46741 cctttcaaac gcaccacgcg cctgccttcg cgggccagcc tgaccataag cgcattggtg
    46801 tcctcttgcg gggtgcggtc gccccgggcg cgcttgccga caaaaatccg ttccgcatcg
    46861 cggcggacga gggacagtat gccgtctgaa accagcgcgt cgtaaagcac cacgtctgcc
    46921 tgctggattt cctgcagccc tttgagcgtc agcagccccg catcgccggg acccgcgccg
    46981 accagcgaga cggagccgcc ttgatcattt tgacgacttt gttccaattg gcctgccaat
    47041 tcccgttcgg caagggtgtt ttgccggttt ttgacgaggg cggcgaaacg tccgttaaac
    47101 tgcttttccc aaaagcggcg gcgttcggta acggatttca gtttgccctt gacggcatcg
    47161 cgccaccttc ctgaaatttc cgccatatcg cccaaagacg gcggcagcag ggcttccagc
    47221 ctttcacgca gcagtcgggc gaggacgggc gcgctgccgg agctggaaac ggcaatctga
    47281 accgggttgc ggtcgataac cgacgggaag atgaagctgc aatggtcgcg gtcgtcaacc
    47341 acgttgaccg gcttttggca gctttcggca agatggaaaa cgcgccggtt gagggcttgg
    47401 tcgctgcttg ccgcaatgat gaggaaaacc gtgcggatgt gttcggcacg aaattcttcg
    47461 gcaagccaca ggattttgtt ttccgccgcc aacgcggaga gttcggcatt caggtgtttt
    47521 gcggcaaccc tgacctctgc gcccgccttc agcagcaggc tgattttgcg tgcggcgacc
    47581 gcgccgccgc ctacgaccaa tacggggcgg ccggcgaggt tggcgaaaat agggaaataa
    47641 ttcactggct gtctcctttg ctgtttgccc gcaccttgtt tccgatacgg tgcgtcgcgg
    47701 catttttgtc ggaatgcggg tcattttaga caaaaggatt ttcccggtta aataataaaa
    47761 aggcatttgt tagaagctga aagctatatg agggcggctg cggatgcggc ggttt
//
LOCUS       DAXHRI010000002        40323 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXHRI010000002 DAXHRI010000000
VERSION     DAXHRI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.002"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(67..3165)
                     /locus_tag="ACFM75_000055"
                     /pseudo
     CDS             complement(67..3165)
                     /locus_tag="ACFM75_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(3196..3308)
                     /locus_tag="ACFM75_000056"
                     /pseudo
     CDS             complement(3196..3308)
                     /locus_tag="ACFM75_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3287..3487)
                     /locus_tag="ACFM75_000057"
     CDS             complement(3287..3487)
                     /locus_tag="ACFM75_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254676.1"
                     /translation="MPFGRVGFSPPKQQQAATTVKQRQAGRWAEAHPTMDTTAPHSAA
                     ANQSSLHIFRQPNPNIKPFIVN"
     gene            complement(3474..4034)
                     /locus_tag="ACFM75_000058"
     CDS             complement(3474..4034)
                     /locus_tag="ACFM75_000058"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6254677.1"
                     /translation="MVWKTLGEIAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSG
                     YVPSEGKMTEYIANDILIGNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQ
                     VLADDKFFAFNMKHAKGAKMPRGSKAAIMQYKIPIPPLPEQEKIVAILDKFDTLTHSV
                     SEGLPYEIALRRKQYEYYREQLLAFR"
     gene            complement(4060..4823)
                     /locus_tag="ACFM75_000059"
                     /pseudo
     CDS             complement(4060..4823)
                     /locus_tag="ACFM75_000059"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(4795..4878)
                     /locus_tag="ACFM75_000060"
                     /pseudo
     CDS             complement(4795..4878)
                     /locus_tag="ACFM75_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible D domain protein"
     gene            complement(4995..6536)
                     /locus_tag="ACFM75_000061"
     CDS             complement(4995..6536)
                     /locus_tag="ACFM75_000061"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6254678.1"
                     /translation="MTEIQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFIS
                     ENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNVAAKAHQNEE
                     LNTNLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGVA
                     ELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEKV
                     NKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNQFH
                     IELGDTLTNPKLKDSKPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKAD
                     FAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFYG
                     TGIAVNILVLSKHKDNTDIQFIDASGFFKKETNNNVLTEEHIADIVKLFADKADVPHI
                     AQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEIGETVAKIERLRREIDEVI
                     AEIEA"
     gene            complement(6613..7617)
                     /gene="rfaD"
                     /locus_tag="ACFM75_000062"
     CDS             complement(6613..7617)
                     /gene="rfaD"
                     /locus_tag="ACFM75_000062"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC6254679.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(7658..8777)
                     /locus_tag="ACFM75_000063"
                     /pseudo
     CDS             complement(7658..8777)
                     /locus_tag="ACFM75_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(8779..10014)
                     /locus_tag="ACFM75_000064"
     CDS             complement(8779..10014)
                     /locus_tag="ACFM75_000064"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6254680.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(10051..11022)
                     /gene="hldA"
                     /locus_tag="ACFM75_000065"
     CDS             complement(10051..11022)
                     /gene="hldA"
                     /locus_tag="ACFM75_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC6254681.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(11078..11818)
                     /gene="pyrF"
                     /locus_tag="ACFM75_000066"
     CDS             complement(11078..11818)
                     /gene="pyrF"
                     /locus_tag="ACFM75_000066"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC6254682.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNIAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPLAVLREVNRVANLEAN"
     gene            complement(12344..12991)
                     /gene="adk"
                     /locus_tag="ACFM75_000067"
     CDS             complement(12344..12991)
                     /gene="adk"
                     /locus_tag="ACFM75_000067"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC6254683.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            13206..14045
                     /gene="htpX"
                     /locus_tag="ACFM75_000068"
     CDS             13206..14045
                     /gene="htpX"
                     /locus_tag="ACFM75_000068"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC6254684.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(14282..15580)
                     /locus_tag="ACFM75_000069"
     CDS             complement(14282..15580)
                     /locus_tag="ACFM75_000069"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC6254685.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEETGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAVDLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGVGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(15684..16835)
                     /locus_tag="ACFM75_000070"
     CDS             complement(15684..16835)
                     /locus_tag="ACFM75_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC6254686.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGTVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            17170..18549
                     /gene="norM"
                     /locus_tag="ACFM75_000071"
     CDS             17170..18549
                     /gene="norM"
                     /locus_tag="ACFM75_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC6254687.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFGALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLELCSR
                     EMVRSHKAV"
     gene            18593..19633
                     /gene="murB"
                     /locus_tag="ACFM75_000072"
     CDS             18593..19633
                     /gene="murB"
                     /locus_tag="ACFM75_000072"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC6254688.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELCDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGIVRIEAQAGEIWHDFVLHT
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPTLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            19645..20295
                     /locus_tag="ACFM75_000073"
     CDS             19645..20295
                     /locus_tag="ACFM75_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC6254689.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            20350..21126
                     /locus_tag="ACFM75_000074"
     CDS             20350..21126
                     /locus_tag="ACFM75_000074"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6254690.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSMGKIIRTVCDNSLSL"
     gene            21177..21743
                     /locus_tag="ACFM75_000075"
     CDS             21177..21743
                     /locus_tag="ACFM75_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC6254691.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            21763..22653
                     /locus_tag="ACFM75_000076"
     CDS             21763..22653
                     /locus_tag="ACFM75_000076"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC6254692.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            22678..23436
                     /locus_tag="ACFM75_000077"
     CDS             22678..23436
                     /locus_tag="ACFM75_000077"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6254693.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(23491..24156)
                     /locus_tag="ACFM75_000078"
     CDS             complement(23491..24156)
                     /locus_tag="ACFM75_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6254694.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(24356..25129)
                     /gene="folE2"
                     /locus_tag="ACFM75_000079"
     CDS             complement(24356..25129)
                     /gene="folE2"
                     /locus_tag="ACFM75_000079"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC6254695.1"
                     /translation="MNTIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVCLTGEMKDGAYGYSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            25321..26478
                     /locus_tag="ACFM75_000080"
     CDS             25321..26478
                     /locus_tag="ACFM75_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC6254696.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFTLHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(26541..27542)
                     /gene="hemB"
                     /locus_tag="ACFM75_000081"
     CDS             complement(26541..27542)
                     /gene="hemB"
                     /locus_tag="ACFM75_000081"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC6254697.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEASNLGIPMLALFPVVTTNKTECAQEAYNPE
                     GLVPTAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHARIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(27725..28009)
                     /gene="yhbY"
                     /locus_tag="ACFM75_000082"
     CDS             complement(27725..28009)
                     /gene="yhbY"
                     /locus_tag="ACFM75_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC6254698.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            28119..28739
                     /locus_tag="ACFM75_000083"
     CDS             28119..28739
                     /locus_tag="ACFM75_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC6254699.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGSSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            28803..30770
                     /gene="ftsH"
                     /locus_tag="ACFM75_000084"
     CDS             28803..30770
                     /gene="ftsH"
                     /locus_tag="ACFM75_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC6254700.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            30992..31423
                     /locus_tag="ACFM75_000085"
     CDS             30992..31423
                     /locus_tag="ACFM75_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC6254701.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            31416..31694
                     /locus_tag="ACFM75_000086"
     CDS             31416..31694
                     /locus_tag="ACFM75_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC6254702.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEVRRKRVQNQEE"
     gene            31747..32325
                     /gene="pth"
                     /locus_tag="ACFM75_000087"
     CDS             31747..32325
                     /gene="pth"
                     /locus_tag="ACFM75_000087"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC6254703.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            32386..32592
                     /locus_tag="ACFM75_000088"
     CDS             32386..32592
                     /locus_tag="ACFM75_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC6254704.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            32589..32951
                     /locus_tag="ACFM75_000089"
     CDS             32589..32951
                     /locus_tag="ACFM75_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC6254705.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFTARAADRPD"
     gene            33182..34597
                     /locus_tag="ACFM75_000090"
     CDS             33182..34597
                     /locus_tag="ACFM75_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC6254706.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            34716..35225
                     /locus_tag="ACFM75_000091"
     CDS             34716..35225
                     /locus_tag="ACFM75_000091"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6254707.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            35388..36770
                     /locus_tag="ACFM75_000092"
     CDS             35388..36770
                     /locus_tag="ACFM75_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC6254708.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(36790..37545)
                     /locus_tag="ACFM75_000093"
     CDS             complement(36790..37545)
                     /locus_tag="ACFM75_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6254709.1"
                     /translation="MIKIRNIHKTFGENTILHGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(37555..38271)
                     /locus_tag="ACFM75_000094"
     CDS             complement(37555..38271)
                     /locus_tag="ACFM75_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6254710.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(38261..39088)
                     /locus_tag="ACFM75_000095"
     CDS             complement(38261..39088)
                     /locus_tag="ACFM75_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6254711.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            39373..40281
                     /locus_tag="ACFM75_000096"
     CDS             39373..40281
                     /locus_tag="ACFM75_000096"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC6254712.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         9530 a        11080 c         9902 g         9811 t
ORIGIN      
        1 tccactataa aaaccgcctc tgcataaaat caaaaatgcc gtctgaaagg ttcagacggc
       61 attttgtcaa atatcggtcc ctattccggc aaacttctcc acaaacgccg caatcttttg
      121 aaaaacactt tgtttcttcg tcagatattg cgggttgatc ggactcattt tcggcagggt
      181 ttcggtcagt tccgtaccgt tttcgctggc atagccgcgt ttgagcgagc tgattaaata
      241 gcgtttggcg gcggtttcgt tcaaaccttc ggcggcaatc aatcctgccg cttcgtgccg
      301 catcacctct tgcgcatagg tgtaaaaggt ttccagtatg gcgggaacgt cgggcacttt
      361 gtccaaatcc gtatcgttga tgaaatccac aatcagccct tctttggcgc ggtgtccgat
      421 acttgcgcgg atgatgcggc ggatttcttc taccagctcc gctttgcctt tgattttttt
      481 gtgatgctcg aaaaccagtt gcaggatgta atccagattg atttcctgcg atttgagcaa
      541 atccacctca aaaaccacat cgtcccagtc gatttttgac tgttctttct gttcgcctgc
      601 tttttggcgg cgcaaccaat cgcggatgtc gttataggcg gaacggtaat cctgcacggc
      661 tctttcagac ggcatcggca cttgccgcat ttcctgcacg tcttcatcgc tcaggtagta
      721 tttttcctga aacgccttca tagcatcttc gtccgccgca tccacattct gcaactcgcg
      781 cagcgcggca aattcatcgt agttctgcaa tacgttttcc gcccgcaggt attcaccgaa
      841 aagtttggca aaatcttttt tgtctttttc cgtttcgatt tcgtggggat cggggaaacg
      901 ttcgcgcaac tcttttgcca catccaaata gccgcgccgc gcttctccgg tcagtctgtc
      961 ggtatagccg ttcatgtatt cttcgtaact tttttccagc actacgtttt tggtgttttt
     1021 atcgccaaac agggtaatcg catcaatggt tgcctgctcc aaatcacgga agcagacaat
     1081 attgccgaag gttttggtgg catcgtaaat gcggttggtg cgcgaaaacg cctgcatcag
     1141 gccgtgatag cgcaggtttt tatcgacaaa caacgtgttc agcgtcggcg cgtcaaaacc
     1201 cgtcaaaaac ataccgacca caatcagcaa atccacttcc tgatttttca cccgttttgc
     1261 caaatcgcgg tagtagttct gaaaggcttt gctgtccgtg ccgaaattgg ttttgaaaca
     1321 ggcgttgtaa tcgttgatgg cagcctgcaa aaattctttt gcgctgctgt ccatcgcttc
     1381 cggttcaaag gtctcgtcaa caatttcacc gacggcgttt tgctcttcgt tggcggcaaa
     1441 ggaaaaaata gtagccactt tcaacgggtg caggctgcct gcctgttgcg ttttgaacgc
     1501 ttcgtaatag cacttcgccg catccacgct gctgacggca aacatcgcgt taaatccttt
     1561 accgccagcg ttcaggcggt gcgttttctg cctgaactga ttcaggatat attgcgtgat
     1621 ttcgcggatg cgttcggggt gcagcagggc ttggcggttt tcggcggcac tcagtttctt
     1681 ctcgtcctgt tccgcttcca cggctttgaa ctgcgggcgc acatcgttgt aatccacttt
     1741 gaatttcaat actttttcat cgcggatggc atcggtaatc acataagaat gcaactcccg
     1801 cccgaacacg cccgccgtgg tttccgcgcc caaagcgttt tcgggaaaaa tcggcgtgcc
     1861 ggtaaagccg aactggcaga attttttaaa tttctttttc aggttttttt gcgcttcgcc
     1921 gaattgcgag cggtggcatt cgtcgaaaat aaagacaact cgctgatggt aaaccggcag
     1981 attatcttca ctcttcatca ggttgttcag cttttggatg gtggtaacga tgattttgtt
     2041 gtcgtctttt tccaaattgc gtttcaagcc cgccgtactt tccgaaccgt tcacactgtc
     2101 gggcgaaaaa cgctggtatt ccttcatcgt ctggtagtcc aaatccttcc tgtccaccac
     2161 gaagaaaacc ttgtcgataa acgccgattc cgtcgccaga cgcgccgcct taaagctggt
     2221 cagcgttttg ccgctgcccg tggtgtgcca aacatagccg ccgctttccg gtttgctcca
     2281 attcttcgcc tgcgccgaac tgttaatttt ccacagaatg cgttcggcgg cggcaatctg
     2341 gtacggccgc ataatcagca gcgtatcatt cgcatcgaac acgctgtaat gcagcaaaac
     2401 gctcagtaat acgctttttt gcaggaacgt ggcggtaaag tctttcaaat ccttaatcgg
     2461 atgattgtcc gaccgcgccc aattcatcgt gaaatcgaag ctgtttttgt cgcgcttggt
     2521 ggtgttggcg aaatagcgcg tgtccgtgcc gttggaaatg acgaagattt gcaggaattt
     2581 gaacaacgaa ttgtcgctgt tgaagctctc tttgctgtaa cggtgcacct gattgaatgc
     2641 ttcacgcacc gccacaccgc gctttttcaa ttcaatctgc accagcggca ggccgtttac
     2701 caacacggta acgtcataac ggtttgcatg cgtgcccgtc tgctcaaact agttgataag
     2761 ctgcacatgg ttgcgggcaa ggtttttctt gtccagcaga taaatgtttt tcagacggcc
     2821 gtcatcaaaa gcgaaatcgt aaatatggtc gtcgtggatt ttgcgggttt tgtcggtaat
     2881 gttttcagac ggcctgtcca gatattccgt taaaaaccgc gcccattcgc cgtctgaaaa
     2941 cgccacatcg ttcagccgct gcaactgcgc gcgcaggttt tccagcagcc tgctttggct
     3001 gttcaagtct ttgcggtatt cgtaaccctg attctgcaaa tcggcgatta actccgcttc
     3061 caactggttt tccgattggt agctgcccga ctgttcgatt ttttcatatt ggtcgagcac
     3121 gatgaaattc ggcgtttcag cgatgggttt agtttcgagg ttcatatggg tcgtcctttg
     3181 cggtgtcggg ttttatagtg aattaaattt aaaccggtac tgcgttggct cgccttgccg
     3241 tactatttgt actgtctgcg gctcgccgcc ttgtcctgat ttaaatttaa ttcactataa
     3301 acggtttgat gttcggattg ggctgcctga aaatgtgcag gctgctttgg ttggctgcgg
     3361 cggaatgcgg ggcggttgtg tccatcgtag gatgggcttc agcccaccgc cccgcctgcc
     3421 gttgttttac tgttgttgca gcttgctgct gtttcggtgg gctgaagccc accctaccga
     3481 aaggcaagca gctgttcgcg gtaatattcg tattgtttgc ggcgcagggc gatttcgtac
     3541 ggcaggcctt cgctgacgga gtgggtcagg gtgtcgaatt tgtctaggat ggcgacgatt
     3601 ttttcctgtt cggggagtgg tgggatgggg attttatatt gcataatcgc tgctttgctg
     3661 ccacgcggca tttttgcacc tttggcgtgc ttcatattaa aggcaaaaaa tttatcatcg
     3721 gccaatacct gatacagata ttttggatta accttttcat ctgttacccg gataaccaaa
     3781 acatcaccgt ttgttccgcc cgtgcagtct gcctgccaga tttttttcaa atacggacga
     3841 atatttccaa tcaagatgtc attggcaata tattctgtca tttttccctc acttggaaca
     3901 taaccggata actttttacc ttctctattt tgtaagagat tatccacgcc cacgtaatta
     3961 tgttcgttca gtttatccga acaaatacgg ttttttgaat attcggcaat ctcccccaac
     4021 gtcttccaaa ccacatcttt cagacggcat tgatagccat cagctatccc ccccgatttg
     4081 attgtcaaaa tctaaaagaa agtcgcggta ataccggtat tggcgtttgc gcagggctaa
     4141 ttccgcttcc agcgtagctt ccagcgtagc ttccagctcg gtgaatttgt caagtatttt
     4201 tacaattttt tgttgggttt ctaggggcgg gatgggaata agtaagtttt gcaattcact
     4261 tttagcaagt gctggtatac ctgctccgta ttgacattgc atgaatgtaa tttgcttcat
     4321 ttttaaaaat aatatgcaaa ccgttgcaat agtttgtctt cgttcgcttt aatagtatat
     4381 gcgtgagctc ctgcaaaaaa agggatatcc atccaattaa caaaccccgc acttgctcct
     4441 ccttggctaa taacaatcgt gccagcttcg ttattgtatt catgccaata acctgatgga
     4501 ttaataccgc cattgattac gggatactta tctttttctc taagtagtga tttattaagc
     4561 tgctttccct ttcctatact tgccacttcc cccaacggct tccactccac cggtgccgtc
     4621 tgaatcatct caatcaattt tttcgcttta ctttgtatat ccatggttgt tttccgtttc
     4681 tttcccgctg cggcgggatg tggggcggtt atccgccgta gggtgggctc cagcccaaca
     4741 atccgcctgc tgttttgccg ttgtttcggg gggctgaagc ccaccctact ttgtctgcaa
     4801 ggtgcacacc gaccaatatt cattgcctgt ttcgtcgatg tgtttggcgt tttcaaaagc
     4861 gttgttttct gttgtcatgt atttttcccc gaaaatttct tgatttgaac ttgtaaagat
     4921 tacttacagg ttaccgcccg tattttcaga cggcctcttt cccattcagt tgttgcaaaa
     4981 tatgcaacaa ctgcctaggc ctcaatctct gcaatcactt cgtcaatttc gcgccgcagc
     5041 cgttcgattt tggcgacggt ttcgccgatt tcggcgttaa gctgtttgat gtcgataatt
     5101 tcgcgtgtgt cttcgggttc aacatagctg ctgacggcga ggttgtagcc gttgtctttg
     5161 acggtttgct gggcggcgtt ttgggcgata tgcggcacat cggctttgtc ggcgaagagt
     5221 ttgacgatgt cggcaatgtg ttcttcggtt aagacgttgt tgttggtttc ttttttaaag
     5281 aagccgcttg cgtcgataaa ttggatgtcg gtattgtctt tgtgtttgga caaaaccagg
     5341 atattgacgg cgatgccggt gccgtaaaag agattgggcg caagggcaat cacggtttcc
     5401 acgtagttgc cctccaccag atattggcgg attttttgct ctgcgccgcc gcgatagaaa
     5461 atgccgggga atgagacgat ggcggcgcgg cctctgccgg aaaggtagtt cagtgcgtgc
     5521 aggatgaagg caaaatcggc tttggatttc ggggcaagta cgcctgcggg ggcaaagcgg
     5581 tcgtcgttga ttaaggtggg gtcgtcgctg cctatccagt tgatggaata aggcggattg
     5641 gaaacgatgg catcaaaggg tttgctgtct ttgagctttg ggttggtcag tgtgtcgccc
     5701 aattcgatgt ggaattggtt gtaattgacg ttgtgcagga acatgttcat gcgggcgagg
     5761 ttgtaggtgg tgtggttgat ttcctgcccg aagaagcctt cttcgatgat gtgctcgtca
     5821 aactgttttt tcgcctgcaa gagcaggctg cccgagccgc aagctgggtc gtagattttg
     5881 ttgactttct cctgcccgtg caccgccagc cgcgcaatca gcttggatac gctttgcggg
     5941 gtgaaaaatt cgccgccgga tttgcctgcg ttggcggcgt agttggaaat caggtattcg
     6001 taggcatcgc cgaaaaggtc gatgtggtgg tcttcaaaac tgccgaaatc aagttccgcc
     6061 acgcctttga ggacggcggc aaggcgtttg ttcttgtcgg caacggtgct gccgagccgg
     6121 ctgctggtgg tgtcgaagtc gtcaaacagg cctttgatgt cttgttcgga cggatagccg
     6181 gaggcggagc tttcaatcgc ggtaaagatt tctttcagat tggtgttgag ttcttcgttt
     6241 tgatgggctt tggcggcgac attgcaaaaa agctggccgg ggtagatgaa atagccttta
     6301 actttgacgg catcgtcttt gatttcgggc gtgatgatgc tgtccggcat agcggcgtaa
     6361 tcaatactgc tgtcgcctgc ctgcatatag tcggtgaagt tttcgctgat aaagcggtag
     6421 aaaagtgtgc cgagaacata ttgtttaaag tcccagccgt ccaccgcacc acgtacttcg
     6481 tccgcgattt tccagatttg gcggtgcagt tgggcgcgtt gttgtatttc ggtcataatc
     6541 gaaatctata taaaagttaa acaaatcaaa atcgcctgat attttcagac gattttttta
     6601 cgggcattca aattaagcca aattttccag catccatttg acgtagcggt cgacgcctga
     6661 tttgacatcg aaaaattctt ccttatatcc ggcttcgcgc aatttggtga tgtcggcttg
     6721 ggtgaagctt tggtatttgc ctttgagtgc gtcggggaag gggatgtagc ggataagttc
     6781 ttcttctacc agctctttca agctcatttc gggtttgcct tcggcggcgc ggcaggcgtt
     6841 gacggtggcg gcggctagtt cgttgaactg ttggctgcgg ccggtgccga gattgtagat
     6901 gccggaaagt tcgggatggt cgaagaagta gaggttgact ttggcaacgt cttcgacgct
     6961 gacgaagtcg cgggtttgtt cgccgttgcc gtagccgtcg ttgctgccga acaggttgac
     7021 gtaaccgtgt tcgcggtatt ggtggaaatg gtggaaggcg acagatgcca tgcggccttt
     7081 gtgttgttcg tgttgtccgt aaacattgaa gtagcggaag ccgacgactt gggcggtgag
     7141 accttctttc atgcggcgac gcaatacttg gtcgaacagg aatttggagt agccgtacac
     7201 gttgagcggt ttttcgagtt cgcgctcttc gcggaagatt tctcctttgc cgtaaaccgc
     7261 cgcactggag gcataaagga aggggatgcg ttcgtcctga caccagtcca gcaaatccag
     7321 cgtgtactgg tagttgttgt ccatcatata caaaccgtcg tggttcatcg tatcggaaca
     7381 cgcgccttga tggaaaacgg cttcgatgtt ttgataaggt aaaatgtgtt ccctcacttg
     7441 gcggatgaat tcgtgtttgt cgagataatg ggcgatttcg cactcggcaa ggtttttgaa
     7501 tttttcgcct ttgctcaaat tatcgacggc aacaatgtca gtaataccgc gttgattaag
     7561 tgctttgacg atgttgctgc cgataaagcc ggccgcgcct gttacgatga tggtcatatt
     7621 cggtttcctt tgttttgagt aaaattaata aacttagtta caagaaatca tcgatattgt
     7681 cctgaaacac aaatttgcga tgatattcca aatatttttc tcttccttcg ggatatattc
     7741 tcatcttaat tttttgcatt tggattgata ttaagttttt cccatgtgta acggcttagt
     7801 cgagtaccgc aaatcaaacg accatcatcc aagaaagtaa tataaccttg atcgaacaac
     7861 caatcataag tcggcgttaa agctaaccca tttaaataat ctaatgcctc tttctcattt
     7921 ttctcagtaa tacaaaccat ataaggttta atatgactag cccttaacaa aatctcatct
     7981 gtaattaatg taaatggaca ttgcgacata tgatttataa catccttgcg atatttctgc
     8041 tgcccttttc tacttttctg tttaatgatt tcttgctcta cgaattgatt aaattctgaa
     8101 gccggtattt ctaatttttc tcttgataag agttgcgggt gcacgataga gcgaaattga
     8161 taatccaaaa atattcggaa ataaaataat ggttctgaat cttctatatc tttgacaggc
     8221 aatagtttaa gaattgataa ataactaatt ttgggcaata caatcttacg ccaaattgac
     8281 catatatcat cctccgaacg gatataaccc ctacggtaac ctttagaatt ttccaaatgg
     8341 tctgatacat catggattga aaagaaaata ttttctgtag gcagattatt aatacttaaa
     8401 acgtaatcat cataaaacct tgaaatatca tgatgatatt tctgttcttg tttgtgatat
     8461 tcaacttttg catcaaccaa ataacgctgt aaatttttct tattaaaaaa acaatttttc
     8521 tgaatgagag gatattctac cctccttcca ttttttgtct cagtaatccc ccaattttca
     8581 tactcaaaaa atgtacttag ttttcgacca ctatcatcaa tataactacc tacatatttg
     8641 cgtgcttccc cattaccacc aaaaacagat aacttgttat tcctgtgaat aaaactatct
     8701 tccgcacgta aattttgaat actatcgaca ataaaatact ctatgttttc aatagtaatt
     8761 ctttgaaaat ccggcatttt actctccata aagcgtaata tccatttgtt ttaatatagc
     8821 gattaataca tcaacaacaa tactgtttcc cgcttggcga tacatttgag tatcactaac
     8881 taaaatttta aaatcatctc taaaacccat tagccgcaaa cattccctag gtgttaattt
     8941 acgaatacgt ccacgattat gagtaacata attatcaacc ccggcacgat gcattttatg
     9001 catagtttgc aataatgggc gagctacagg caaatcagtt tccgtactgg ttttaaaatt
     9061 ttttgttcct cctgccaaaa cataattttt gattttttca gaaagataat atttttcctc
     9121 aacgttattt acatcaaaaa taaagtcatc aaattcagat tcgcgggctg cctgaaaaat
     9181 aaaatcacca tgccaattaa attgttgatt tgctttttgg cataaggcaa tttctccatt
     9241 aatctgtgta taacgttttt gtctattttt agaactggta acaaatttca cacccttttc
     9301 acgtaaaaaa tatttgctat cagtatagtc ctccaaaaaa tcttgcatag tatgttctaa
     9361 ttcaatcttt tcaggaaact gaaaaccatt tataggaggg gtatgaaagc caacaacaaa
     9421 aatacgctca cgatgttgag gaatcccata atccttacta ttcattattt ggaaatataa
     9481 gtcataacca agtgaataaa aaacactttt tacaactttc caagtttttc cattatcatg
     9541 attgagcaag ccctttacat tttcataaat aaatatcttt ggttggactt catccacaac
     9601 gcgagcaaat tcatagaata atgttcctcg tgtatcttct aatcctgcac gtttgccaac
     9661 catggaaaat gcttggcaag gactgcctcc aactaaaata tcaacttgat ttctaaactt
     9721 tctcgcatca aattgagtaa tgtcgttatg ccaaaaatct tcatttaatt tatagtttcc
     9781 aagataactt tttttaacgt atggatcaat atctcctgca aaaacaatgg tatggtttaa
     9841 atttaatcgg tggaatgcct gttcaaccgc accaatcccg ctgaatacgg ttgctaatct
     9901 aatatgtgaa tcaggtttaa gaaaagtttt agatttccaa ccttgttgac tgggaaagag
     9961 caaagttttt tgtaatcgag tatcgtgtgt ctgtgccatt gtcgaaatag tcatacttat
    10021 atcgttctgt ttatcttatc aatatgaaaa ctacattgtt gattgccctg acaatgcctt
    10081 gatcaattcg gcaaacgagc aaaccgccgt accgagtttc gccacgacaa ccccggccgc
    10141 agtattggca aggtacatgg cttcgggcat ggtgcagcct gccgccaaac ccaagcccat
    10201 tccggcaatg acggtgtcgc ccgcaccgga tacgtcgtaa acttcttggg cgcgggtggg
    10261 ctggtaaatc ggttcgcctt cgctgaacaa ggtcatgcct tcttcgcttc gggtcagtaa
    10321 aacggcggtc aggtcgaggt ggcggcgcag gttttgcgct ttttcggtca gctcgctttc
    10381 gtttttccaa ctgccgacca cttctttcaa ttcggcgcgg ttaggcgtaa tcagagttgc
    10441 accgacatat ttttcgtaat cgtcgccttt ggggtcgatt aagacggttt tgccggcgtg
    10501 tttcgcccaa tcgatcatat cggagatatg cgacaggccg cctttgccgt agtctgaaaa
    10561 aatgattgcg tcgtattcgg gcaagatttc gcggtatttc tgcttgattt gttccaacac
    10621 ttcgcagttg ggatgttctt caaaatcaag acggataagc tgctggttgc gggcgacgac
    10681 gcgcagtttg acggtggtgg cgatttgttt gtcgcgcatc agataggagg cgacgccgtc
    10741 ctgcaccatc agcgcatcga gcgcgtcggc ggcttcgtcg ttgccggtta cggacaacag
    10801 ccctgccctg ccgcccaacg aagcgatgtt gcgcgcgaca tttgccgctc cgcccgcgcg
    10861 ttggtcgatt cgtccgattt tcgccaccgg cacgggggct tcgggcgaaa tacgggacac
    10921 atcgccgaac caatagcggt cgagcatcac gtcgccgaca accaggactt tggcttgcgc
    10981 gaaacgggat ttgagggttt cttgttggaa cttggcggac atattgcctc cgttgtgttt
    11041 tgttcagacg accttaaccg gtaaggccgc ctgaaaatca gtttgcttca aggtttgcca
    11101 cgcggttcac ttcgcgcaat acggctagcg gatcggcagc ttgggtaacg ggacggccca
    11161 tcaccagata ggtcgaacct gccgccaagg cttcggcggg cgtcatgatg cggcgttggt
    11221 cgtcgttgtt gccggcaacg tccaagcgga taccgggcgt gaccaagaca aaatcctgtc
    11281 ccaattcgcg gcgcagcggt gcggcttctt gggcggagca gaccacgccg tccaagcccg
    11341 aactttgcgc cagtttcgcc aaacggatga cttgttcttc gggagcgatg ttcaaaccga
    11401 tttccgccaa atcgctttgt tccatgctgg tcaacacggt tacgccgatt aagagcggct
    11461 ttgtaccgta tccggcaacg gcttctgctg cggcttccat catacggcgg ccgcccgacg
    11521 cgtgcatatc gaccatccaa acgcccatgt cggcagcgac tttgcaggct tgcgcgacgg
    11581 tgtggggaat atcgtggtat ttcaaatcga ggaaaagttt gaaaccttga ttgattaagc
    11641 tttctgccaa attgcgtccc gtcgcggtaa acagctcttt gccgattttg atttgacaca
    11701 atgtcgggtc aaggttgcgg acgaatccga gcgtgtcttt ttcgttggaa aaatcaaggg
    11761 cgacgatgac gggggtgcgt tgttgcggag tttggaagtc ggagattaaa ggattcatgg
    11821 tgttttcgtt tcgggttttc agacgacctt tatcgtcttt ttcgcagtat agtggattaa
    11881 ctttaaaccg gtacggcgtt gtctcgcctt agttcaaaga gaacgcttct ctaaggtgct
    11941 gaagcaccaa gtgaatcggt tccgtactgt ttgtactgcc tgcggcttcg tcgccttgtc
    12001 ctgatttttg ttaatccact ataacaacgt aaattcaaac cgtaggtcgg gcattgatgt
    12061 ccgacctacg cggactcaag cctccgacaa actgtttcgg ataaatttga accggtacat
    12121 cagccgtcat tcccgcgcag gcgggaatcc attgataaaa ctcagttcat cagatttaaa
    12181 cagcgattgc ccaaattcag aaatggattc ccgcctgcgc gggaatgacg ccaaatgatt
    12241 atttttcggt tgcaacacgc tgtttatcaa aaacaggtcg tctgaaaacc tgattttctg
    12301 ttttcagagt gcgaagcctg cccgtgggta cgaccttttc gatttatttg cccaatgcgc
    12361 ccaatacttc ggctttcacg gcttctaccg gctgagtgcc gtcaactttg atgtatttag
    12421 gcgcgtgttc gccttccagt ttgctgtaaa aatcgaccaa aacttcggtt tgctcgtggt
    12481 aaacggcaag gcgttttttc acggtttctt ctttgtcgtc gtcgcgctga atcaaatctt
    12541 cgccggttac gtcgtctttg ccttcaactt tgggcgggtt gtaggtaacg tggtaagtac
    12601 ggccggaagc caaatgcacg cggcggccgc tcatgcggtc gacaatcacg ctgtcaggca
    12661 catcgatttc aacgactgca tccaaatcca cgcctgcttc aaccatcgct tcggcttgtg
    12721 ccaatgtgcg cgggaaaccg tcaaacagga aaccgttttt gcagtcgtct tgcgcgatgc
    12781 gttctttgac catgccgata atgatgtcgt cgcgcaccaa gccgccttcg tcaatgattt
    12841 ttttcgcttc caaacccaag ggcgtgcctg ccttaatcgc ggcacggagc atgtcgccgg
    12901 tagagatttg cggaatgccg aacgctgcgg tgatgaattg cgcctgagtg cctttgcccg
    12961 cgcccggtgc gcctaaaagt aatgctttca tgaagtgatc cttttaaatg tgtttattgg
    13021 aatcaaacag atggtttacg attctacacg gcttggcaca atagttcaaa aaccaactgc
    13081 acaaactgcc atttgcaagc aaatgttttg catacctttg ccgagcctga tattcacaag
    13141 gcggcaactt tttgggacaa tccccaaaat ttcaaaacat cacaccctac caaaaaggaa
    13201 catccgtgaa acgcattttt ctgtttttgg ctaccaatat cgctgttttg gtcgtaatca
    13261 acattgtttt ggcggttctg ggcatcaaca gccggggcgg cgcgggcagc ctgttggcgt
    13321 attccgccgt cgtcggcttc actggttcga ttatttcgct gctgatgtcc aaattcatcg
    13381 ccaaacaatc ggtcggcgcg gaagttatcg acacgccgcg caccgaagaa gaagcctggc
    13441 ttttgaacac tgtcgaagcc caagcgcggc aatggaacct gaaaacgccc gaagtcgcca
    13501 tctaccactc ccccgaaccc aatgcctttg ccacgggcgc atcgagaaac agctccctga
    13561 tcgccgtcag caccggtttg ctcgaccata tgacgcgcga cgaagtggaa gccgtattgg
    13621 cgcacgaaat ggcacacgtc ggcaacggcg atatggttac gctgacgctg attcaaggcg
    13681 tggtcaatac tttcgtcgtg ttcctgtcgc gcattattgc caacctgatt gcccgaaaca
    13741 acgacggcag ccagtcccag ggaacttatt tcctagtcag catggtattc caaatcctgt
    13801 tcggcttcct tgccagctta attgtcatgt ggttcagccg acaacgcgaa taccgcgccg
    13861 acgcgggcgc ggcaaaactg gtcggcgcac cgaaaatgat ttccgccctg caaaggctta
    13921 aaggcaaccc ggtcgatttg cccgaagaaa tgaacgcaat gggcatcgcc ggagatacgc
    13981 gcgactccct gctcagcacc cacccttcgc tggacaaccg aatcgcccgc ctcaaatcgc
    14041 tctgagccga tttgaaacaa caaaaaaacc gtacgcgcaa gcagtacggt tttttcatat
    14101 atgccgtctg aaaatatcgg gtttgccata ccggcaccga ccttaccaat ttccgcaccg
    14161 gatacgccgc attgatatac cctgataaac cgtgtccaaa cagaaaatat cagataaaac
    14221 ggcagcaaaa atgccgtctg aaaccaatta gggcttcaga cggcattttt actgcaaaac
    14281 cttatgcgaa cggatgatgc agcacaatcg tttcttctcg gtcggggccg gtggagacga
    14341 tggcgaccgg cgcaccgcag acttcttcaa tccgtttcaa atatgctttg gcgttttcgg
    14401 gcaatgcgcc gtagtccttc acgccgaaag tggattcacg ccagccgggc atggtttcgt
    14461 aaatcggctt gcaggtttcc accgcatcgg aaccgcaagg caggatgtcg gttttgccgc
    14521 cgtcgggcaa ttcatagccg acgcagatat tgatggtttc aacgccgtcc attacatcga
    14581 gtttagtaat acacataccg gaaatgccgt tgatttggat ggagcgtttc agggcggcgg
    14641 catcaaacca gccgcagcgg cgtgcgcgac cggttaccga accgaattcg tgtccgcgtt
    14701 ctgccaaacc tacgcctact tcgtcgaaca attcggtcgg gaacgggccc gaaccgacgc
    14761 gcgtggtata ggctttgacg atgcccaaaa cataatccag catttgagga cctacgcccg
    14821 cgcctgcgga agcggctccc gctagacagt tggacgaggt aacgaaggga taagtgccgt
    14881 agtcgatgtc caacaacgca ccttgcgcgc cttcaaacag cagtttttcg ccgtttttgt
    14941 ttttctcgtt caacacgcgg gacacgtcgg taatcatcgg cgcaatgcgc ggcgcgactt
    15001 tttcgataac cgccatcacg tcttccgctt taaccggctc ggcattgtgc agatgttgca
    15061 gttggacatt gtaataggca aggacggcat ccagtttttc acgcagtttt tcaggatgca
    15121 gcaaatcgac ggcgcgaatg gcgcggcgtg ccactttgtc ttcgtaggca gggccgatgc
    15181 cgcggccggt cgtgccgatt ttgcctttgc cgcgcgatgc ttctcgggct tggtcgagcg
    15241 cgatgtgata aggcaggatc agcgggcagg tcggcgcgat tttcagacgg ccttcgacgt
    15301 ttttcacgcc tgcagcgttc aactcgtcga tttcgcccaa cagggcttcg ggggagacga
    15361 caacgcccga accgatgaag caatccaatc cttcgtgcag gatgccgctc ggaatcaggc
    15421 gcaaaatggt ttttttgccg ccgacaacca aggtatggcc cgcattgtga ccgccttgaa
    15481 agcgcaccac gccgccggtt tcttccgcca gccagtcaac aattttacct ttaccctcgt
    15541 cgccccactg tgcgccgatt actacaacat ttttagccat agccatataa cctatcgata
    15601 ttaaaaatta ttgcggaaat ccccaaactg gtgcggcttt gcgccccgcc acgatttgcc
    15661 tttcatccgc catgaacggg tatttagcgt ttcacgacct gccaaacgcc gtccgtcttt
    15721 ttcagacggc ctgcaagctc ttccgaaaca ttgtgtccga taccgtaatc gattacgaca
    15781 cactgccctt gttcacgcaa ggcttcgacc gcttcgcgcg ccgcttcggc atcttccgca
    15841 tcgaccaaca cggcgggctg ccgttcgatg gcgggcaaac gcccgataaa gctgcgcaag
    15901 tcgaaactga atcccgttgc cgggcgcgcc ctgccgaaat atccgcccaa tccgtcataa
    15961 cgcccgccgc gcgcgaccgc gtcgtggaaa tcggcggcat aggcggcata cagcaagccc
    16021 gtgtggtaat tgtcgacacg cagctcggac aagtcgatat ggatttcaca atcggggaat
    16081 gcgtcgcaca ccgcctgcaa ttcgtctaac gcgccgccga ccgccgacaa atccggcaac
    16141 cgtccgcgcg cgtcggacaa cacttcacgc ccgccgtata ggcgcggcag cagcgagaat
    16201 gcttttgccc acatgccgtc cagcttccaa gccttgacct gcgcttcgac cgtcccggta
    16261 tctttatcct gcatcaaggc aagcagcgtt gcagactgcc ccgcatccaa atgcgccgca
    16321 tcggacaagg cgcgaaatat gccgatatgc cccagcgaaa gcagcacttt gcccatatcg
    16381 gcaattttca tgcttttcag catcaggtct atcagctcga tgtcgccacg gatgtcggca
    16441 aaaccgtaca tttccgcccc tgcctgcaag ggttcgcgca tattcagcag accgtcgggc
    16501 tgcgcgtgca acaccggacc ggcataacac aaccgattaa tcccttggtt ggcggataaa
    16561 agatgggcat cgatacgcgc cacctgcggc gtgatgtcgg cgcgtatgcc caactgcctg
    16621 ccgctgagcc tgtccgttac caaaatggtt ttcagggaaa gccccgcatc gatatgcgtc
    16681 agcagggaat gtgcgtactc catcagcgga ggctgtacca gttcataacc gtgtacgcgg
    16741 aacagtgcca acaactgctc cctcgcgctt tcaagctgcc gcgcgttcgt gggcagtacg
    16801 tcggcgatat gttccggaag ctgccatgtc tgcatgagaa tctgccttct attttatcct
    16861 gtaaacacaa aagactgccc gatccgcaat cagacggcat ctttgccgca acgcatcacg
    16921 ccgctgtcaa aacggcgatg cttctttccg attcagaatc cgaacagccc ctgtccgacg
    16981 gctcaatgcc gtcaagtcgt taaaaccaaa ctttaccata aaatacacac aatctgaata
    17041 tacttttata ccctgtatcc gccgtctgac ggcacggcat ttttttacta tataatgtgc
    17101 accattattt ctcatcactt cctgctgccg ttttctgctt cagacggcat ttttgttgat
    17161 tgaacactta tgctgctcga cctcaaccgc ttttcctttt ccgtcttcct gaaagaagtc
    17221 cgcctgctga ccgctcttgc cctgcccatg ctgttggcgc aggtcgcgca ggtgggcatc
    17281 ggttttgtcg ataccgtgat ggcgggcggt gcgggcaagg aggacttggc ggcggtggct
    17341 ttgggcagca gcgcgtttgc cacggtttat attaccttta tgggcattat ggcggcgctg
    17401 aacccgatga ttgcccagct ttacggcgcg ggtaaaaccg acgaagtggg cgaaacgggg
    17461 cggcagggga tttggttcgg gctgtttttg ggcgtgttcg gcatggtctt gatgtgggcg
    17521 gcgattacgc cgttccgcaa ctggctgacc ttgagcgatt atgtggaagg cacaatggcg
    17581 cagtatatgc tgttcaccag cttggcgatg ccggcggcaa tggtacaccg cgcactgcac
    17641 gcctacgcct ccagcctgaa ccgcccgcgc ctgattatgt tggtcagctt tgcggcgttt
    17701 gtgttgaacg tgccgctgaa ctatattttc gtttacggca aattcggtat gcccgctttg
    17761 ggcggtgcag gctgcggact ggcgacgatg gcggtgtttt ggttcggcgc gctggcattg
    17821 tggatttata tcgctaagga gaaattcttc cgtccgttcg gactgacggc gaaattcggc
    17881 aaaccggatt gggcggtgtt caaacagatt tggaaaattg gcgcgcccat cgggctgtct
    17941 tattttttgg aagccagcgc gttttcgttt atcgtgttct tgatcgcgcc cttcggcgag
    18001 gattatgtgg cggcgcagca ggtcggcatc agtttgtcgg ggattctcta tatgattccg
    18061 caaagcgtcg gctcggcggg gacggtgcgc atcggctttt cgcttgggcg gcgcgaattt
    18121 tcgcgggcgc gctatatttc gggcgtgtca ctggtgttag gatggatgct cgccgtgatt
    18181 accgtgcttt ccttggtatt attccgttcg ccgctggtaa gtatgtacaa caatgatccg
    18241 gcggttttaa gcatcgccgc caccgtctta ctgttcgccg gcttgttcca accggcagac
    18301 ttcacccaat gtatcgcctc ctacgccttg cgcggctaca aagttacaaa ggtgccgatg
    18361 ttcatccacg ccgccgcctt ttggggctgc ggtctgctgc cgggctacct gctcgcctac
    18421 cgtttcgata tgggcattta cggcttctgg acggcattga ttgcctcgct caccatcgcc
    18481 gccgtcgcct tggtgtggtg cttggaattg tgcagcaggg agatggtcag atcgcataag
    18541 gctgtctgaa aacgcagtac acttcaattc aaatacagtt aaagttcaaa ccatgcaacc
    18601 catccgatac cgaaccgacc tcacccccta caacaccttc ggtcttcgcg cccaagcccg
    18661 agcctttatc gcgctcgaac atgccgacga gttgtgcgac atcgtccgac tgcccgagtt
    18721 cgaccgcgat actgttttat ggctgggcgg cggcagcaac atccttttga tgcaggatta
    18781 cgccggactg gtcgtacaca tggaaaacaa aggcatacgc gagattgcgc gttcagacgg
    18841 cattgttcgc atcgaagcgc aggcgggcga aatctggcac gattttgtcc tgcacaccgt
    18901 cgcgctgggc ttgaatgggc tggaaaacct gagcctgatt ccgggtacgg tcggcgcgtc
    18961 gcccgtgcag aacatcggcg catacggcgt agaggcgaaa gatgtgattc acagcgtgcg
    19021 ctgcttcgat ttggatacgg aaacctttgt aaccctttcc aatgccgact gccgcttcgc
    19081 ctaccgcgaa agcctgttca agcaggaggg taaagggcgt tatgtgattg tttcggtcgt
    19141 atttgcatta aaaacgcatt ttgtgccgac tttgggttac ggcgatttgg cggccgccgt
    19201 tgccgaactg agcgcgggca gggtcccgac ggcgaaagat gtttccgatg cagtgtgtgc
    19261 aatccgcaac agtaaacttc ctaatcctaa cgtgcttggc aatgtcggca gtttctttaa
    19321 aaaccccgtc gtcagcgcag aaaaagccgc caccttgttg cagcggcatc ctgatatgcc
    19381 gcattatccg cagccggacg gttcggtcaa actcgccgcc ggctggctga tcgaccaatg
    19441 ccgtctgaaa ggcttccaaa tcggcggcgc ggcggtacac gacaaacagg cattggtttt
    19501 ggtaaacaaa aacaacgcct cggcaaacga tgtccggcag ttggcgcaac acatcaaatt
    19561 tacagtattt gctcggtttc aggtagaatt gcacgccgaa cccaattggc tgcctgcttc
    19621 gttcagcctg taaatccaag gagtatgccc gtgacccgcc ccgccaaaat caatacttac
    19681 acacgcatca tcgacgccag ccttgcgctt ttcaacgagg aaggcgagcg caacatcagc
    19741 accaaccata ttgccgccca cttgggcatc agtccgggca atctctatta ccacttccgc
    19801 aacaaagacg aaatcatcgt ccaactgttc aaacgttaca gcgaagccct gctggaatac
    19861 ctcaacgaag ccgtgttgcc gtctgatgtg gaagactcca tcaactatat ggcgggcatt
    19921 tacgacgtga tgtgggaata ccgcttccta ttcagcgacg tgaacaccct gcttgcgcgc
    19981 agtgctgaat tattgggcga acacaatacc ttcacccaag ccaaagtctc cccgctcttg
    20041 gtcaacctgc ttacccagct caacggcttg aacatcatcc aagccgacca aaccgctatg
    20101 aacgacctcg ccgtcaatat gtggatggtc acgaaatact ggttcgactt cgacagctcc
    20161 ctgcgcggcc gcaccaagct gaccgaagac tccaaagcac gcggcgtccg tcgtacctta
    20221 agcctccttc gtccttatct tttgcccgaa caccgcgaga aatacgaccg gaaaatcggc
    20281 aacggcaacc cgtaaaccca cagcgtccga atcagccttc agacggcact tacccgacgg
    20341 tatcagcgaa tgcgcgcagt ccccccgccc catatctcca tcacaggcct aggttacctc
    20401 ggcctgccgc tggcacaaaa gttttaccaa cacggcagcc gcgtcgccgc cataaaacgc
    20461 agcctgactt cggacgacat caacctgccc atacatctcg acaccttcga cctcggcagc
    20521 accgatgcct ttcagacggc attgtggcgg caccacgccg acaaacccgt ctggttcttc
    20581 ctgctaccgc cctcctcgct gacgcattac gccgacaccg tcaaacaatg ggcagaactt
    20641 gcccgggcgt gcaacgtgca acacctgatt ttcacaagca gcaccagcgt ttacggcgat
    20701 acagcgcgcg aatgcgacga aaccgccaca cccgatccgc aaaccgagtc cgcccgccaa
    20761 atcctcgccg ccgaacaaca cctgctcgac agcggcgttc cgaacatcga catcctgcgg
    20821 ctgggcgggc tttattgcgc cgaacgccat cccgtcagcc gccttgttca aaagcaaaac
    20881 atcccgggcg gcaaccgccc catcaacatc gtccaccgta atatcgccgt cgaaagcctg
    20941 tttcagacgg catttaaccc cggcggcagg cggctgaaaa acattatcga accgcgccac
    21001 ccgacacgac gcgaattcta tacggaagaa gccgccaaac tcggcttgcc ggctcctgat
    21061 ttcgcacccg acgacagcat gggcaaaatc atccgtaccg tttgcgataa cagcttaagc
    21121 ctgtaaaata agtgtcaaca gcaacaaaca ggcattctcc gccccgatca gaaaatatgt
    21181 ccgccctcct ccccatcatc aaccgcctga ttctgcaaag cccggacagc cgctcggaac
    21241 ttgccgcctt tgcaggcaaa acactgaccc tgaacattgc tgggctgaaa ctggcgggac
    21301 gcatcacgga agacggtttg ctctcggcgg gaaacggctt tgcagacacc gaaattacct
    21361 tccgcaacag cgcggtacag aaaatcctcc aaggcggcga acccggggcg ggcgacatcg
    21421 ggctcgaagg cgacctcatc ctcggcatcg cggtactgtc cctgctcggc agcctgcgtt
    21481 cccgcgcatc ggacgaattg gcacggattt tcggcacgca ggcagacatc ggcagccgtg
    21541 ccgccgacat cggacacggc atcaaacaaa tcggcaggaa catcgccgaa caaatcggcg
    21601 gattttcccg cgaatccgag tccgcaaaca tcggcaacga agcccttgcc gactgcctcg
    21661 acgaaataag cagactgcgc gacggcgtgg aacgcctcaa cgaacgcctc gaccggctcg
    21721 aacgcgacat ttggatagac taaccttcag acggcatccg acatgaacag cccttttcac
    21781 aacatcggca tcgtaacccg ccccaacacg cccgacatcc aagacaccgc acacacgctg
    21841 attacctttt tgaagcagca cggctttacc gtctatctcg acgaagtcgg cataaaggaa
    21901 ggctgcatct atacccaaga caccgtcggc tgccatatcg tcaacaagac cgaactgggg
    21961 caatactgcg acctggtcgc cgttttaggc ggagacggca cctttctctc cgtcgcccgc
    22021 gaaatcgccc tgcgcgccgt tccgattatc ggcatcaacc aagggcattt gggcttcctg
    22081 acccaaattc cccgcgaata tatgacggac aagctattgc ccgttttaga agggaaatac
    22141 cttgccgaag agcgcatcct gattgaggcc gcactcatcc gcgaaggcaa aaccgccgaa
    22201 cgcgccatcg ccctcaacga tgccgtcctc tcccgtggcg gtgccggaca gatgattgag
    22261 tttgaagtct tcgtcaatcg ggaattcgtc tatacccagc gttcggacgg gctgattgtc
    22321 tccaccccca ccggatcgac cgcctattcg cttgccgccg gcggccccat catgcaggca
    22381 ggattacacg ccttcacgct cgtccccatc tgcccgcaat ccatgaccaa ccgccccatc
    22441 gccattccag acacgtccga aatcgaaatc ctcgttaccc aaggcggcga cgcgcgcgtc
    22501 catttcgacg gtcaaaccca tatcgacgtg caaaacctcg accgcatcac catccgccgc
    22561 taccgaaatc ccttacgcat tttgcacccg accgactacc aatatttcaa aaccctgcgc
    22621 caaaaactgc actggggcga gcaattagtc taagccggcc tttacccgaa agtatccatg
    22681 aatcccaaaa tttcctccga ccatacagaa gatgccgtgc gcctctccaa acgcatggcg
    22741 caactggggc tttgttcgcg ccgcgaagcc gacggctaca tcgaacaggg ctgggtaacg
    22801 gtcaacggca aaaccgccgt actcggtcag aaagtttcac cggcagaccg tatcgaactg
    22861 aacaagaaag cccacgaaca gcaggcggca cgcattacca tcctgttgaa caaacccgtc
    22921 ggctatgtca gcgcacaagc ggaaaaaggc tataaatccg ccgccgaact gattacccct
    22981 gaaaatcact gggaaggcga taccggccgc atccgtttcg atccgaaaca caaaatcggc
    23041 ctcgcccccg ccggcaggct ggacatcgac tcggtcggat tgctggtatt gactcaggac
    23101 ggccgtatcg ccaagcagct tatcggcgaa aacagcggca gtgaaaaaga atatttggtg
    23161 cgcgtgcgcg gcaaattgga cgaaaaagga cttgccttac tgaatcacgg attgagtttg
    23221 gacggcgaga aactgcgtcc cgcccaagta gaatggcaaa acgaagacca actgcgcttc
    23281 gtgttgaaac agggtaaaaa gcggcaaatc cgccgtatgt gcgaactggt cggactgcgc
    23341 gtcgtcgggc tgaaacgcat ccgcatgggc aaggtcaaac tcggcaggct gccgcccggc
    23401 aaatggcgtt atctcgcccc cggtgaatcg ttttaaataa gcattaaaaa atgccgtctg
    23461 aaaatccttg cgttttcaga cggcattgcc ttatgcccaa ataacggttt cttccaaggg
    23521 cctgcgcgcc ttcgtggcga tttcctgaac gcggtaaccg aatgtcgcgg cgacggacac
    23581 gccccattct gccgcatcga acaaaccgaa ctgcccggac aatacgcgtt ccatatcggc
    23641 atagttgaaa ccttccaccg ggcaggaatc gatacctgcc atcgccgcac ccgtcatcat
    23701 gttgcccaac gcgatatagg tctggcggca acaccagtca aacaaggcgc gcgaatcgtc
    23761 caaaatcttg atgtcgtcgg cttgaaacgc ctgatacctc gccaaagatt ttgccatggc
    23821 atccggttcg gtaacgccgc gccgtttgag gctttccaac ataaacgggc tgtcgaagcg
    23881 ggcatttttc tttgccaaaa acaccaccaa atgactggcg gtatccaaag catccgccat
    23941 accccaagaa aacggcttga ttgcctgtcg gatttcgggg ttttgaacca caacaaactg
    24001 ccaaggctcc gaaccgaccg aactgggcga caaacgcccg agttctaaaa taaactgaaa
    24061 atcctcggca ctgattttac gtgccgcatc gtaatgccgg catgatttgc ggtttttaaa
    24121 tgcggataga acctgctctt tgcttaatac tgccatcgca catcctttca ttacttgtat
    24181 tgcaaattga tttcaaaccg atacggcact gccgcaccat acaatccgtc ctcgccatca
    24241 agcttcatcc ggactttacc catctctcta cggctgggaa aacaaagcgg cggcagactc
    24301 gggaataacg ccccaatgcc tttcagacgg catttgggca tcattgcaaa cgcgcctacg
    24361 ggtaggcgat ataggcataa gccgaatggt tgtggataga ctcgaaattc tcgctctcga
    24421 caacgaaact cttgatgcgt ttgtcggcga tcagcgaagt agcgacatca cgcaccatat
    24481 cttccacgaa tttcgggttt tcgtaggctt tttcggtaac atatttttca tcggggcgtt
    24541 tgagcaggcc gtagagttgg cagcttgcct gcgcctccac ataatcaatg acttcctcga
    24601 taccgacttc tgcatcggca gtcaggctga cggtaacatg cgaacgctgg ttgtgcgcgc
    24661 catattggga aatttctttg gaacacgggc aaagcgaggt tacgggaatc atgaccttca
    24721 tactgtagcc gtatgccccg tctttcattt cgcccgtgag acagacatca taatccagta
    24781 aggaccgaat accggaaacc ggcgcggttt tcttgcggaa aaacggaaaa gaaacgctga
    24841 ttttgccggc gcgggaatct aaaagcgcga ccatttcggc agtcagcctg tgcaattgtg
    24901 caaaatccaa ggcttcagaa tgttgctcca tcaatgcgac aaaacgcgac atatgcgtcc
    24961 ccttctgctc agcaggcagg taaaccgtca tggtcagacg ggcaacggtg gactggatgc
    25021 cttctgcagt ttgcagggta atcggaaagc gcaggtcttt gataccgacc tgattgatcg
    25081 gcagattgcg caaatctcgg ctggattgca cgtctgcaat agtgttcatg agttgttttt
    25141 ccttaatatt catgtaattg ttgagatgtg tgcatggtat ggcgtttccg ccggggaata
    25201 taccgtcaat ctgcaaatgc gagattatat cactcgcttt tagcagctgc attgattgtt
    25261 tctatttttc agatagtgat aaaatcttcg attattcaca tctacatagg cagagaccaa
    25321 atgaaattcg ccacgaaagc cattcattcc agctacgatt gcgacgaaca caaccgcgcg
    25381 ctgatgccgc cgatttatca aaacagtatg tttacgttgc acgagattgg cgaaaatgtg
    25441 ccttaccgtt attcgcgcct gagcaacccg acccgtcaga ttttggaaga caccgttgcc
    25501 gatttggaac acggtgcggc aggctttgcg ttttccagcg gtatggcggg gattgatgcc
    25561 gtatggcgta ccttcctgca accgggcgat accatcgtcg cggtcgccga tatttacggc
    25621 ggcgcgtatg acttattggt cgatgtttat caaaaatggg gggtaaatgt tgtttttgcc
    25681 gatttaggca atcccgacaa tttggacgaa ttgctcaaag cgcacaaggt caaactggtt
    25741 tggctggaaa cgccgtccaa tccgctttta cgcttggtag acatcaaagc ccttgccgca
    25801 aaagccaaag cgaccggcgc gctggtcggc atcgacaaca cctttgccac gccgtatctg
    25861 caacagccgt tggatatggg ttgcgatttt gtgttccatt ccgctaccaa atatttgtgc
    25921 ggccattccg acgtattgat gggcatcgtc gttgccaaaa ccaaagagct ggctcagcct
    25981 ttgcacgata tgatggtgca taccggtgcg attgcaggcc caatggactg ctggctggtg
    26041 ttgcgcggca tcaaaacgct tgccctgcgt atggaggcac actgcaaaaa cgcactcgaa
    26101 atcgcgcgcc gtctggaaga ccatcctgcc attgaaaaag tgttccatcc cggcctgccg
    26161 tcgcacgaac attatgaact cgcccaagcg caaatgccta aaggcatcgg cggcgtggtg
    26221 acggtttatc tcaaaaacga cacgcgcgaa gcggcaaaca gcgtgattaa aaacatgaaa
    26281 ctggtcaaaa tggcttccag cctcggcggc gtggaaagtt tggtcaacca ttgctattcc
    26341 cagtcccaca gcggcgtgcc gcataatgtg aaaatggaaa tgggcatcaa agtcggcctg
    26401 ctgcgtttct ccatcggcat cgaagacgcg gacgatattt ggaacgatat ttccgccgca
    26461 ctcgatacga ctttgtaaac tgtaaaaatg ccgtctgaaa ccatggtttc agacggcatt
    26521 tcaattaacc cggccgaaaa tcaacgcttc aacatctttg ccgcctcaat agcgtaatag
    26581 gtcaaaatcc cgtccgcacc cgcacgtttg aatgccagca ggctttccaa aaccactttg
    26641 ccgccgtcca gccagccgtt ggcaatcgct gcctgcaaca tcgcgtattc tcccgaaacc
    26701 tgataggcat aagtcggtac gccgaactcg tccttcacgc ggcggacaac gtccaaatac
    26761 ggtaaaccgg gcttcaccat caccatatcc gcaccttccc gaatatcgag cgccacttca
    26821 ttcagcgcct catcggtatt tgccggatcc atctggtagg ttttcttgtc cgccttgccc
    26881 aaattgcccg aactgcccac cgcatcgcgg aacggcccgt aaaacgccga agcatatttg
    26941 gcggaatacg ccataatccg cgcgtggata tgcccggcat cctccaacgc ctcgcgaatc
    27001 gcaccgatac gcccgtccat catatcggaa ggggcaacca cctgtgcgcc cgcttcagcg
    27061 tggcacaaag cctgcttgac caaaacctct acggtttcat cgttcatcac ataaccgttt
    27121 tcgtccgtca gcccgtcctg accgtgcacc gtataaggat cgagcgcgac atccgtcata
    27181 atgcccagtt cgggaaacct ctcgcgcaag gctcggacgg ctgtcggcac cagtccttca
    27241 gggttatacg cctcctgcgc acattctgtc ttatttgtcg taacgacagg gaatagtgcc
    27301 agcatgggta ttcccaaatt tgacgcttcc tccgccgtaa acagcagcct gtccaaactt
    27361 tgacgcttca cacccggcat agaaggcaca tcctcctcgc gcgccgaccc ctccaatacg
    27421 aacaccggat aaatcaaatc atcggcggtc agcgtgtgtt cgcgcatcag gcggcgtgaa
    27481 aaatcgtccc tgcgcatacg gcgcatacgc gaagccggaa cattgcggta aggaaactgc
    27541 ataagccctc caatcatcct gtcaacaatt caaaccatac ggaagccgcc gcaacttgca
    27601 gacagcatcg ggcggcaatc aaaatccgtt ttcaccggaa agggtttcgg cccgccgaaa
    27661 tgccgcgccg aaggacggcg tcccgcccat taaagaacaa tagcaaaaca acagcttcag
    27721 gctgtcagcc ttcgatattc ttacgccaca ataccaaaag tttcccgata tgctgaacag
    27781 gttgcgcgtc aaccgcctca cataaggcag tgcagatttc gatacgttcg gcacggtcgt
    27841 cgccgaatac gcgcacttta atcagctcat gcgccgtcaa tgccgcatcg gtttccttga
    27901 tgaccgcgtc cgtcagaccc tgctgaccga ccatcacaac aggatggaga tggtgcgcgc
    27961 gcgctttcag ttccaaaatt tctttggtgt tcaatttggt atccgtcatt ttcctactgc
    28021 ttgaataaaa agaataatgc ggcattgtac gcgatttggc acgacatcgg caaaattaac
    28081 gtacaatacg cggtttccat tccgacccgc gaaccactat ggctgtacgt tccaaatcct
    28141 caaaagcgtg gctgcacgaa cacgtcaacg accactacgt ccatatggcg caaaaagacg
    28201 gctaccgcgc ccgtgccgca tacaaacttt tggaaatcaa cgacaaagac aaattaatca
    28261 aacccggcac ggtacttgcc gacttgggca gcgcgccggg aagctggtcg caggttgccg
    28321 ccaagctgac gggttcttcc ggagcagttt tcgccttgga catcctgcct atggaagcca
    28381 tagggggcgt ctccttcatt cagggcgact tccgcgagaa cgacgtactg gcacaattcg
    28441 aaaccttgtt ggacaaccgc ccgctcgacc ttgtaatttg cgatatggcg cccaatatgt
    28501 cgggaaacgc cgtaagcgat caggcacgca gcttttatct gtgcgaactg gctttagact
    28561 tcgcctcgca acacctgaaa accggcggca gctttttggt caaagtcttt cagggtgcag
    28621 gctatcagga atacatggca gccatgcgcg aaattttcgg cacggtgcag acgcgcaaac
    28681 ccgaagcctc gcgcaatcgc tccagtgaga tttatttatt gggcaaaaat aaacgctgac
    28741 aatacagacg gcgtgcttta caatcatttc cgttttacac ctcaattatg gagccttgct
    28801 aagtggggaa cacctttaaa tcaatccttg tctgggtcgc cttgggtatc ggcctgatgg
    28861 ctgcgttcaa cgctttagac ggtaaaaaag aagacaacgg gcaaatcgaa tattctcagt
    28921 tcatccaaca ggtcaacaac ggcgaagtat ccggcgtcaa catcgaagga tccgtcgtca
    28981 gcggctacct gattaagggc gagcgcaccg acaaaagcac cttcttcacc aacgcgcctt
    29041 tggacgacaa cctgattaaa acactgctcg acaaaaacgt ccgtgtaaaa gtaacgccgg
    29101 aagaaaaacc gagcgcgctg gctgccctgt tttacagcct gctgcccgtc ctgctgctga
    29161 ttggcgcgtg gttctacttt atgcgtatgc agacgggcgg cggcggcaaa ggcggcgcat
    29221 tctcattcgg caaaagccgc gcccgcctac tggacaaaga tgccaacaaa gtgacctttg
    29281 ccgatgtcgc cggctgcgac gaagccaaag aagaagtgca ggaaatcgtc gattacctca
    29341 aagcgccgaa ccgctatcaa agcctcggcg gtcgcgtgcc gcgcggcatc ctgctggcgg
    29401 gcagcccggg tacgggtaag acgcttttgg caaaagccat tgcaggcgaa gccggcgtgc
    29461 cgttcttcag catttccggt tccgactttg tcgaaatgtt cgtcggtgtc ggtgcgagcc
    29521 gcgtccgcga tatgttcgag caggcgaaga aaaacgctcc ctgcatcatc tttattgatg
    29581 aaatcgacgc agtcggccgc caacgcggcg caggtttggg cggcggcaat gatgagcgcg
    29641 agcaaacatt aaaccaattg ttggttgaaa tggacggttt tgagagcaat cagactgtaa
    29701 ttgtgattgc ggcaaccaac cgccccgacg tactcgatcc tgcgctgcaa cgccccggcc
    29761 gtttcgaccg ccaagtggtt gtccccctgc cggacatccg ggggcgcgaa cagattttga
    29821 acgtccattc taaaaaagtg cctttggaca aatctgtgga tttattgtcc ctcgcgcgcg
    29881 gcacgccggg tttttccggc gcggatttgg cgaacttggt caacgaagcc gccctgtttg
    29941 ccggccgccg caataaagtc aaagtcgatc agagcgattt tgaagacgcc aaagacaaaa
    30001 tctatatggg tccggaacgc cgcagtatgg tgatgcacga agacgaaaaa cgtgcgacgg
    30061 cgtatcacga atccggacac gcgattgttg ccgaaagcct gccctttacc gaccccgtcc
    30121 acaaagtaac cattatgccg cgcggacgtg cgctgggtct gacttggcag cttccggagc
    30181 gcgaccgcat cagtatgtat aaagatcaga tgttgagcca gctctccatc ctgttcggcg
    30241 gacggattgc cgaagacatc ttcgtcggac gcatctccac cggcgcatca aacgactttg
    30301 aacgcgcaac ccaaatggcg cgcgaaatgg taacgcgcta cggcatgagc gacaaaatgg
    30361 gcgtgatggt ttatgcggaa aacgaaggcg aagtcttctt gggacgcagc gtaacccgtt
    30421 cgcaaaacat ttccgagaaa acccagcagg acatcgatgc ggaaatccgc cggattttgg
    30481 acgagcagta tcaggttgcc tacaaaatcc tcgatgaaaa ccgcgacaag atggaaacga
    30541 tgtgcaaagc cctgatggaa tgggaaacca tcgaccgcga tcaggtactg gaaatcatgg
    30601 cgggcaaaca acccagcccg cccaaagatt acagccacaa cctgcgcgag aatgcggacg
    30661 cggcggaaga taacgcgccg cacgctccga ctcgggaaga aaccgaagca cctgccccgg
    30721 cagacaccgc ttcgacagag tccgagcagc agcctgaaaa caaggcttaa cttcccgaac
    30781 aaacggcagc ccgcaagctg ccgttttccc atcctgtttt cagacggcat tttccgctca
    30841 atgccgtctg aaacgccaaa gccgtatcgg tttttgcgaa tctgcgatat ggcggtttaa
    30901 attgaaatca gaacggggca aggctgtacc ggtttaaaat taaaccgcta taataccgcc
    30961 cttcgaatac acgcccgcat tggtgatgcc cgtgaaaaaa gttgaaaaaa acatcttggt
    31021 gctgcacggc gcggacaaaa tgttcgagct ggtcgataag gttgaggatt acccgaactt
    31081 tctgccgtgg tacagcaaaa ccgaggtcat cgggcgcaac ggcaacgaac tgaaggcgcg
    31141 gctgtttatg gactatatgc acgttcgcca atcattcgcc acgcacaacc gcaacattcc
    31201 gggcagggaa atccgtatgg aactgctcga aggtccgttc aaaaccttac gtggaacgtg
    31261 gaaatttatc gatttgggcg acgatatgtg caaaatcgaa ttcaatttgg aatacgattt
    31321 ttccaatgcc gttttgtccg ccttaatttc ccccgtcttc aaccaccttt ccgccacgct
    31381 ggtcgaagcg ttcgtcaaag aggcagaccg ccgttatgct tgaaattgag attgtgtacg
    31441 ggctgcccga ccgacaggtt ttgaaaacca tgcagcttgc cgagggaaca accgtccgcg
    31501 ccgccgcact gcaaagcggt ttggacggca tatttgaaga tttaaacctg cattccgcgc
    31561 ctttgggcat tttcggcaaa gccgtcaaag acgacacgcc cctgcgcgac ggcgaccgca
    31621 tcgaagtgta ccgcccgctg ttgatcgatc ccaaagaagt gcgccgcaaa cgcgttcaaa
    31681 atcaagaaga ataaccatgc cgtctgaagc cttcagacag cattcgacag aaacacggaa
    31741 aatatcatgt caaacaaaat caaaatgctt gtcggattgg gcaaccccgg caaagaatac
    31801 gaacaaaccc gacacaatgc gggtttttgg ttcctcgacg aactggcgtg gaaatggaag
    31861 gcttcattta aagaagaaaa aaaattcttc ggcgaagtcg cccgtgccgc cctgcccgac
    31921 ggcgacgttt ggctgctcaa acctgccacg ttcatgaacc gttccggaca ggcagttgcc
    31981 gcgcttgcac agttctacaa aatcaaaccc gaagaaatcc tcgtcgtcca cgacgaactc
    32041 gacattccct gcggacggat taaattcaaa ctcggcggcg gcaacggcgg acacaacggc
    32101 ttgaaagaca ttcaggcaaa actcggcacg gcagactatt accgcctgcg cctcggtatc
    32161 gaccacccgg gcgaccgcaa cctcgtcgtc ggctatgtcc tgaacaaacc cagtacggaa
    32221 caccgccgac agattgacga tgccgtcgcc aaatccctgc aagccatacc cgacatcctt
    32281 gccggcaaat gggaagaagc aacccgcttc ctgcacagca aatgacccga tgccgtctga
    32341 agccctttca gacggcattt tcccgatttc catatccgaa cagtcatgac cgaactcaag
    32401 cagcttatcc aaaccgaatc catccccgtc atcgaagaaa ccctcgattt cctgctgtac
    32461 gaatgcagca tcgacgacgc accatccgcc gaagaagtgg cacaatggcg cgacatactt
    32521 gccgcacgcg gcggcaaatt cctgcgcctg tccaaaatct gccaaacgtg gctggacgag
    32581 gaggcggcat gaagctgccg cgcaaccgct tcagcctgct ttccgcattg tggtttgccg
    32641 gcggcatcta ttcgctgctc ttcaaagctg ccgacaccgc gccgccgccg tttccgcatt
    32701 tcgacaaagc agcacacctt gccctgtttt tcgcacaaat ctggcttttg accaaagcat
    32761 tcaaaaccgg aaaacttccc atcccctacc gcagcctgat ggtctttgcc ctctgtttcg
    32821 ccctcttcag cgaatgcgcg caggcatggt ttaccgcaac gagaaccggc agtttgggcg
    32881 atgtccttgc cgacctgacg ggcgcagccc ttgccctctt taccgcgcga gctgccgacc
    32941 gcccggactg aatcggttta ttttccccaa acaggatgca cctcttcccg atacatccta
    33001 tttttcccct tacatatcaa tcccatctca taaacaaaac cgacaaatcg tttacaatat
    33061 atttacacta catcagatta caaatatact cgaaccagtt gcagaatcgg cggttccaca
    33121 aaccgtttcg gattgcacga cacaaaagaa aaaccgattt tattgcctta aaggagtatt
    33181 catgaacctg catgcaaagg acaaaaccca gcatcccgaa aacgtcgagc tgctcagtgc
    33241 gcagaagccg attaccgact ttaagggcct gctgaccacc attatttccg ccgtcgtctg
    33301 tttcggcatt taccacatcc tgccttacag ccccgatgcc aataaaggta tcgcgctgct
    33361 gattttcgtt gccgcacttt ggtttaccga agccgtccac attaccgtaa ccgcactgat
    33421 ggtgccgatt ctcgccgtcg tactcggttt ccccgacatg gacatcaaaa aggcgatggc
    33481 tgatttttcc aacccgatta tctacatttt tttcggaggc ttcgcgcttg ccaccgccct
    33541 gcatatgcag cggctggacc gtaaaatcgc cgtcagcctg ttgcgcctgt cgcgcggcaa
    33601 tatgaaagtg gcggttttga tgttgttcct cgttaccgcc tttctgtcca tgtggatcag
    33661 caacaccgcc accgccgcga tgatgctgcc tctagcaatg ggtatgctga gccacctcga
    33721 ccaggaaaaa gaacacaaaa cctacgtctt cctcctgctc ggcatcgcct attgcgccag
    33781 catcggcggc ttgggcacgc tcgtcggctc gccgcccaac ctgattgccg ccaaagccct
    33841 aaatctggac ttcgtcggct ggatgaagct cggcctgccg atgatgctgt tgattctgcc
    33901 cttgatgctg ctctccctgt acgtcatcct caaacctaat ttgaacgaac gcgtggaaat
    33961 caaagccgaa tccatccctt ggacgctgca ccgcgtgatc gcgctgttga ttttccttgc
    34021 cacagccgcc gcgtggatat tcagctccaa aatcaaaacc gccttcggca tttccaatcc
    34081 cgacaccgtt atcgccctga gtgccgccgt cgccgtcgtc gtcttcggcg tggcgcaatg
    34141 gaaggaagtc gcccgcaata ccgactgggg cgtgttgatg ctcttcggcg gcggcatcag
    34201 cctgagcacg ctgttgaaaa catccggcgc gtccgaagcc ttgggacagc aggttgccgc
    34261 caccttttcc ggcgcgcccg catttttggt gatactcatc gtcgccgcct tcattatttt
    34321 tctgaccgag ttcaccagca acaccgcctc cgccgcattg cttgtaccga ttttctccgg
    34381 catcgctatg cagatggggc tgcccgaaca agtcttggta ttcgtcatcg gcatcggcgc
    34441 atcttgtgcc ttcatgctgc cggttgccac accgcctaac gcgattgtgt tcggcacggg
    34501 cttaatcaag caacgcgaaa tgatgaatgt cggcatactg ctgaacatcc tctgcgtagt
    34561 attggttgct ctgtgggctt atgctgtact gatgtaaacc atcgacctaa acaacaagac
    34621 cgtctgaaag aatatttttc agacgacctt gaagtttgtc gttcagacac aatttgtcga
    34681 atcattcaaa accagattct aacgaaagga aacccatgat tatcctgcac accaacaaag
    34741 gcgacatcaa aatcgaactc gatttcgaca aagcccccgt taccgccaaa aacttcgagc
    34801 aatacgtcaa agacggcttc tacgacggcg taatcttcca ccgcgtcatc aaaggcttca
    34861 tgattcaagg cggcggcatg gatgaaaaca tgaacgaaaa agaaacccgc gatccgattc
    34921 aaaacgaagc gtccaacggc ctgcccaacg ataaatacac catcgccatg gcacgcactt
    34981 ccgaccccca ttccgccagc gcgcaattct tcatcaacac tgccgacaac gctttcctga
    35041 acttccgttc taaagagctg tacggcaaaa ccgtcgtcca agactggggc tatgcagtat
    35101 tcggtaaagt cgttgacggt tttgacgttg tcgatgccat cgaaggcgta tccaccaaac
    35161 gccacggcta ccatgacgac gtaccgagcg aacctgtcgt catcattaaa gccgaagcgg
    35221 tataaaccgg caatccgaaa gcagcctgca caaaggctgc tttttttgtt tcaggcggca
    35281 taaaatccaa gcacatatgc cgtctgaaca cctcccgcaa gccttcggac aatgttgcaa
    35341 cgtgttaaaa tagcctcctt tcctgaaacc acaacaagga aaccatcatg gcaaatattg
    35401 cccgcgacat cttcaaagcc tacgacatcc gaggcattgt cggcaaaacc ctgactgacg
    35461 atgccgccta tctcatcggc agggccatcg ccgccaaagc cgccgaaaaa ggcatgaccc
    35521 gcatcgcgct cggacgcgac ggacgcttga gcggtcccga actgatggag cacatccaac
    35581 gcggctttac cgacagcggc atcggcgtac tcaacgtcgg catggtcgcc actcctatgc
    35641 tctattttgc cgccatcaac gaatgcggcg gcagcggcgt gatgattacc ggcagccaca
    35701 atccgcctga ttacaacggt ttcaaaatga tgctcggcgg cgacacgctc gcaggcgaag
    35761 ccattcaaga acttttagct attgttgaga aagatggttt tgttgccgcc gacaaacaag
    35821 gcaacgtaac cgaaaaagac atctccggcg aataccacaa ccacatcgtc ggccacatca
    35881 aactcaaacg ccccatgaaa attgccatcg acgcgggcaa cggcgtgggc ggcgcgtttg
    35941 ccggcaaact ctataaaggc ttgggcaacg aagtaaccga gcttttctgc gacgtggacg
    36001 gcaatttccc taatcaccac cccgaccctt ccaaaccgaa aaacctgcaa gatttgattg
    36061 ccgcgctgaa aaacggtgat gccgaaatcg gcctggcgtt tgacggcgat gccgaccgct
    36121 tgggcgtggt taccaaagac ggcaacatca tctatccgga ccgccaactg atgttgttcg
    36181 cgcaagacgt gttaagccgc aatcctaaag ccaaagtgat tttcgacgtg aagtccaccc
    36241 gcctgcttgc gccttggatt aaagaacacg gcggcgaacc tgtgatggaa aaaaccggcc
    36301 acagctttat caaatccgcc atgaaaaaaa ccggcgcgcc ggttgccggc gaaatgagcg
    36361 gacacatctt cttcaaagaa cgctggttcg gcttcgacga cggtctgtac gccggcgcac
    36421 gcctcttgga aatcctgtct gcctccgata atccgtccga agtgttaaac aacctgccgc
    36481 aaagcatttc cacgcccgaa ctcaacatcg ccctgcccga aggcagcaac ggccatcagg
    36541 ttatcgacga actcgccgcc aaagccgaat ttgaaggcgc aaccgaaatc atcaccatcg
    36601 acggtctgcg cgttgaattt cccgacggct tcggtctgat gcgtgcttcc aataccacgc
    36661 cgattctggt gttgcgtttt gaggcggata cgcaagaagc catcgagcgg attcaaaacc
    36721 aattcaaagc cgtcatcgaa agcaatccga atctaatctg gcctctgtaa acacaggaaa
    36781 aatgcttaat caaatcttgg tagattggat ttggcttaaa aatctccgcg tccgttcgtg
    36841 tttggggtgg tcgaacaact ctttcgggct gccctgctct acgataacgc cgccgtccat
    36901 cacgacaacg gtcgtggcaa cttccagcgc gaacttgatt tcgtgggtaa cgacgaccat
    36961 cgtccaacct tcccgcgcca attctttcat ggcgttcaac acgtcttgca ccaactcggg
    37021 gtcaagcgcg gaagtgggtt cgtcaaacaa catcagctcg ggctgaatcg ccaatgctcg
    37081 ggcaatgccg acgcgctgct gctgaccgcc ggaaagctgg tagggataca aatccacttt
    37141 gtcgcccaag ccgacttttt ccagcagttt cagagcctct tcgcgcgctt gggcggcagg
    37201 cttgccctgt acggcaaccg gtccttccat cacgttttcc aaggcggttt tgtgcggaaa
    37261 gaggttgtat tgttgaaaca ccatgcctga tttgcggcgc agtgccaaaa tatcgtgttt
    37321 gcttggtttt ttagaaaaat cgattttcag cggtcgctcg ttgtcgaact cgatttgtcc
    37381 gtcttcgggc atttccaacg cgtttaggca gcgcagaaac gtcgttttgc ctgagccgga
    37441 aggcccgagg atgacgacca cctgcccttt gcacacatcc aaatcgatgc cgtgcaaaat
    37501 ggtattttcg ccgaaggtct tatggatatt gcggatttta atcatgacaa ctccttattt
    37561 ggcgacgtag cggtcgaaac gtttttccaa acgcgcctga atcaggaaca gcactttaca
    37621 aaaacaccag taaaccaaag cggcttcgat atagacgggc aaaaagtcat aagtgcggtt
    37681 tgccgtttcc tgcgcgacgc ggaataattc cgttaccgtc acgactgccg cgagcgaggt
    37741 gtttttaaac aaaccgataa actcgttgct caaaggcggc acggcaacgc ggaatgcctg
    37801 cggcgcgaca atgcggcgga acgtctgcat ataggtcatg ccgatggaga aacctgcttc
    37861 ccattggcct ttcggtacgg acaaaattgc cgcgcgtatg gtttcggaag catatgcgcc
    37921 gacattgagc gaaaagccga tgatggcggc aggaatcggg tcgatataga tgccgacgga
    37981 aggcagcccg taaaacacaa tcacaagctg aaccaacagc ggcgtaccgc gaatgacgga
    38041 aatataaaat tccaccaatt tcagcaggat tttccgcacg atgccgccgg cgggcataat
    38101 ccgcaccaaa gccacggcta ccgcaatcat cataccgata acgaaagaag ctgccgccaa
    38161 aggcagagag accgcgaagc cggctttgac cataggcaaa aacgcgctga caatcatatc
    38221 ggcgcgtgtt tccgtcataa acggcagcga agcaaggaaa ttattgaaca ctgatgtctt
    38281 ttccgaagaa ttgttcgccc agttttttca gcgtaccgtc ggctttcagc tcgttgattg
    38341 ccgtactgaa tttcgccacg gcttcgtcat tgcccttgtt gacaatcagg ccggaaccga
    38401 ctttttcatc ggcaggtgcg gaccaaacga ttttcacgcc cgcattcggg tttttcttca
    38461 gatagtccaa aaccgccaat tcgtcgttca gggttgcatc ggcacgtttt tgttcaatca
    38521 gggtcagcga ttgcgccaaa ccgtcaacag ccaccaaatc tgcgcctgca gctttggctt
    38581 tttcgccgta gttgctggtc agggattgtg cggttttcac gcctttgatg tcgtcgatag
    38641 atttgatgtt gctgtcgtta cgggcaacca atacggcacc gctccagctg taaggatcgg
    38701 atttgtcgaa tgtcgcttgg cgttcggggc tggtcagacc gacttggttt gccaccacgt
    38761 cgaaacgccc cgccttcaaa cccgccatca tcgaatccca ttgcgtttct ttaaactcga
    38821 ctttcacgcc cagtttttcc gccacggcgc gggttacttc cacatcgtaa ccggtcagtt
    38881 tgccgtcctt gtcgtggtag gtaaacggtg cgtaagtgcc ttccgtgccg acggtaaccg
    38941 tgcctttatt gttgatgcgc tcgattaaag aaccggaaac tgccgattgt gcaggcgcgg
    39001 aagatgctcc gctgccgctt tccgaaccgc cgcaggccgc caaaaccaat gcggcaatgc
    39061 cgccgagtac gaattttttc aacatcattc tctcctgtaa aaagtgtgca agtgcggcat
    39121 tctaagtgaa attgcggcaa acacaaagaa tacttttttt atatggttat tccaaatcgg
    39181 aatggaaaag atatgccgaa gccgccggaa gcggaaataa agggggcggg aaaggcggcg
    39241 ggcggcacga aaccgaaaag cggcatcagg tatgcaaacg gcggattcac gcacgccgtt
    39301 ccggcaaaaa tccgcctgcc gattcggtgc tataatccat ccgaaacaga tgacaccaaa
    39361 aaggaacatc tcatgaacac gccccaatcc gccatcattc ccgaccacgc ccaagccggc
    39421 atctttatcg aagccgactt cgccgccaac cgcctcaacg atattaaagc cgcctgccgc
    39481 gcttcgctcg acgcgttatc cgccttgaaa acccgctttc cggacgatat tttgggtctg
    39541 accatcgcct tcggcagcaa agcctgggca acattcggac atacggacga aggcagtgaa
    39601 atcaaaccct tctccgaaat gggcaacggg cttgcgccgt ccacgcagca cgatatgtcc
    39661 atccacatcc aatctttccg ccaaaacgcc gcctacgcgc ttgcccaatc cgttttgggc
    39721 gcgttcggcg acagcatatg cgtcgcgtcc gaagaacacg gtttgcgcct gtatcaggat
    39781 cgcgggctgg acggtttcgt cgacggcacg gaaaacccgc agggcgatga aaatgtccgc
    39841 gaagtcgcca tcatccccga aggaaaaccc gatgcgggcg gcagctatgt cctgctgcaa
    39901 aaatacctgc acgatttgaa aaaatgggat gccgtccccg tcgccgaaca ggaggcctcg
    39961 gtcggacgcg gcaaggaaac cgacgacgaa ttcggccgcg atgtccgcct gcccgattcg
    40021 cacctcggcc gcgtcaacct gaaagaaaac ggagtcggcc tgaaaatcgt ccgccgcagc
    40081 ctgcccttcg gcaaaatcag cggcgaacac ggtttgatgt ttaccgcata ctgccgcacg
    40141 ctgcacaata tcgaagcgca attattgagt atgttcggcg atacggacgg caaaaccgac
    40201 ctgctgctcc gacatctctc cgccgccgtt tcgggcggat actactacgc gccgtctgtc
    40261 gaacgcctgc aaaacctcta agccggacga tgccgtctga aaccattttt cagacggcat
    40321 ttt
//
LOCUS       DAXHRI010000003        38500 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXHRI010000003 DAXHRI010000000
VERSION     DAXHRI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.003"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(142..232)
                     /locus_tag="ACFM75_000097"
     tRNA            complement(142..232)
                     /locus_tag="ACFM75_000097"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Ser,seq:gga)
     gene            complement(268..354)
                     /locus_tag="ACFM75_000098"
     tRNA            complement(268..354)
                     /locus_tag="ACFM75_000098"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(318..320),aa:Leu,seq:cag)
     gene            complement(390..476)
                     /locus_tag="ACFM75_000099"
     tRNA            complement(390..476)
                     /locus_tag="ACFM75_000099"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Leu,seq:cag)
     gene            565..2949
                     /gene="rnr"
                     /locus_tag="ACFM75_000100"
     CDS             565..2949
                     /gene="rnr"
                     /locus_tag="ACFM75_000100"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC6254713.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQF
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPIPIEVQKVKKRKGKS"
     gene            2949..3218
                     /locus_tag="ACFM75_000101"
     CDS             2949..3218
                     /locus_tag="ACFM75_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254714.1"
                     /translation="MLTGRLGEGRAGHTFPKPPPDFLIWRPAHHFRPIETPVLKDCVY
                     FHRRFKGRRYSADWAQTAFRRHFHSFGVSVRIALPVRAISPCGLP"
     gene            complement(3293..4756)
                     /gene="guaB"
                     /locus_tag="ACFM75_000102"
     CDS             complement(3293..4756)
                     /gene="guaB"
                     /locus_tag="ACFM75_000102"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC6254715.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4957..5037)
                     /locus_tag="ACFM75_000103"
                     /pseudo
     CDS             complement(4957..5037)
                     /locus_tag="ACFM75_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5083..5544)
                     /locus_tag="ACFM75_000104"
     CDS             complement(5083..5544)
                     /locus_tag="ACFM75_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254716.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNTVFNSLKSSFEEKYYAGGNNGLDFREQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(5635..8193)
                     /gene="glnD"
                     /locus_tag="ACFM75_000105"
     CDS             complement(5635..8193)
                     /gene="glnD"
                     /locus_tag="ACFM75_000105"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC6254717.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAALETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSTVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETSNHSRRISRRSRYMPIAPGITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQTLLEQLS"
     gene            complement(8242..8562)
                     /locus_tag="ACFM75_000106"
     CDS             complement(8242..8562)
                     /locus_tag="ACFM75_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6254718.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(8886..9359)
                     /gene="bfr"
                     /locus_tag="ACFM75_000107"
     CDS             complement(8886..9359)
                     /gene="bfr"
                     /locus_tag="ACFM75_000107"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6254719.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(9387..9851)
                     /gene="bfr"
                     /locus_tag="ACFM75_000108"
     CDS             complement(9387..9851)
                     /gene="bfr"
                     /locus_tag="ACFM75_000108"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6254720.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(10315..11298)
                     /gene="lipA"
                     /locus_tag="ACFM75_000109"
     CDS             complement(10315..11298)
                     /gene="lipA"
                     /locus_tag="ACFM75_000109"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC6254721.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(11291..11914)
                     /gene="lipB"
                     /locus_tag="ACFM75_000110"
     CDS             complement(11291..11914)
                     /gene="lipB"
                     /locus_tag="ACFM75_000110"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC6254722.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIANFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11916..12191)
                     /locus_tag="ACFM75_000111"
     CDS             complement(11916..12191)
                     /locus_tag="ACFM75_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC6254723.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            12376..13446
                     /locus_tag="ACFM75_000112"
     CDS             12376..13446
                     /locus_tag="ACFM75_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC6254724.1"
                     /translation="MYRRKGRGIKPWMGACAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(13553..14500)
                     /locus_tag="ACFM75_000113"
     CDS             complement(13553..14500)
                     /locus_tag="ACFM75_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC6254725.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSIVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(14515..14922)
                     /locus_tag="ACFM75_000114"
     CDS             complement(14515..14922)
                     /locus_tag="ACFM75_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC6254726.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIIYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            15003..15662
                     /gene="ung"
                     /locus_tag="ACFM75_000115"
     CDS             15003..15662
                     /gene="ung"
                     /locus_tag="ACFM75_000115"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC6254727.1"
                     /translation="MDTWHDALGNEKRQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDINGFSI
                     PDHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            15739..15867
                     /locus_tag="ACFM75_000116"
     CDS             15739..15867
                     /locus_tag="ACFM75_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6254728.1"
                     /translation="MAIKKNQLYASLWAGCDELRGGMDASLYKELCAYAIVFKICF"
     gene            15809..16958
                     /locus_tag="ACFM75_000117"
                     /pseudo
     CDS             15809..16958
                     /locus_tag="ACFM75_000117"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            17306..18250
                     /locus_tag="ACFM75_000118"
     CDS             17306..18250
                     /locus_tag="ACFM75_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6254729.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     CLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            18401..18526
                     /locus_tag="ACFM75_000119"
     CDS             18401..18526
                     /locus_tag="ACFM75_000119"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254730.1"
                     /translation="MLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT"
     gene            complement(18816..19331)
                     /locus_tag="ACFM75_000120"
     CDS             complement(18816..19331)
                     /locus_tag="ACFM75_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254731.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(19797..21716)
                     /locus_tag="ACFM75_000121"
     CDS             complement(19797..21716)
                     /locus_tag="ACFM75_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6254732.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQTLQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAE
                     KEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQEE
                     LERIETEIEKQFAER"
     gene            complement(21793..22164)
                     /locus_tag="ACFM75_000122"
     CDS             complement(21793..22164)
                     /locus_tag="ACFM75_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC6254733.1"
                     /translation="MLFEENPIDGHFTEYGHRSGEIRLAGQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            22361..23668
                     /locus_tag="ACFM75_000123"
     CDS             22361..23668
                     /locus_tag="ACFM75_000123"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC6254734.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            23661..24095
                     /locus_tag="ACFM75_000124"
     CDS             23661..24095
                     /locus_tag="ACFM75_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254735.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(24154..24423)
                     /locus_tag="ACFM75_000125"
     CDS             complement(24154..24423)
                     /locus_tag="ACFM75_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC6254736.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(24605..27055)
                     /gene="lon"
                     /locus_tag="ACFM75_000126"
     CDS             complement(24605..27055)
                     /gene="lon"
                     /locus_tag="ACFM75_000126"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC6254737.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            27155..27331
                     /locus_tag="ACFM75_000127"
     CDS             27155..27331
                     /locus_tag="ACFM75_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HFC6254738.1"
                     /translation="MPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVLPPYFQ
                     NMTQVFHVFFYFSR"
     gene            27306..28409
                     /locus_tag="ACFM75_000128"
     CDS             27306..28409
                     /locus_tag="ACFM75_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6254739.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLGLRPADFLEKAFIRAGLRVADMM
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(28437..30182)
                     /gene="recD"
                     /locus_tag="ACFM75_000129"
     CDS             complement(28437..30182)
                     /gene="recD"
                     /locus_tag="ACFM75_000129"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC6254740.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSSDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTVLMLQLLKAVKTGARVILLGDENQLP
                     SVGIGDVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPLLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGGLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSRGLLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(30250..30945)
                     /gene="lolD"
                     /locus_tag="ACFM75_000130"
     CDS             complement(30250..30945)
                     /gene="lolD"
                     /locus_tag="ACFM75_000130"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC6254741.1"
                     /translation="MSELILKCESVGKRYRDGGLDVPVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFGRVMVM
                     KDGSLHPKQGANA"
     gene            complement(30938..32185)
                     /locus_tag="ACFM75_000131"
     CDS             complement(30938..32185)
                     /locus_tag="ACFM75_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC6254742.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            32390..32668
                     /locus_tag="ACFM75_000132"
     CDS             32390..32668
                     /locus_tag="ACFM75_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254743.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(32724..33341)
                     /gene="recR"
                     /locus_tag="ACFM75_000133"
     CDS             complement(32724..33341)
                     /gene="recR"
                     /locus_tag="ACFM75_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC6254744.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(33408..34946)
                     /locus_tag="ACFM75_000134"
     CDS             complement(33408..34946)
                     /locus_tag="ACFM75_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC6254745.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     ASVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(35006..35380)
                     /locus_tag="ACFM75_000135"
     CDS             complement(35006..35380)
                     /locus_tag="ACFM75_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC6254746.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQVPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            35530..37158
                     /locus_tag="ACFM75_000136"
     CDS             35530..37158
                     /locus_tag="ACFM75_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC6254747.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGTIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            37230..38462
                     /locus_tag="ACFM75_000137"
     CDS             37230..38462
                     /locus_tag="ACFM75_000137"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC6254748.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYGFEIAEETMKLMRQM
                     VANGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAANMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAA"
BASE COUNT         8728 a         9884 c        10308 g         9580 t
ORIGIN      
        1 taccgcaaaa cataccacaa aaaacggtaa cggatagttg taagcggttg atgacgatta
       61 tagacaataa cgggttttcc caatgaaatt attgtttgaa taataaaaaa tcccaaaccg
      121 taaaagtttg ggatttgtat ttggcagaga ggaagggatt cgaaccctcg atacgctatt
      181 cacgtataca cgctttccag gcgtgcgact taaaccactc atccacctct ctaatggcgg
      241 aaattatccc aatcggaata atttatttgg tgcccgggag aagactcgaa cttccacacc
      301 cgtgaggata ccagcacctg aagctggcgc gtctaccaat tccgccaccc gggcaatcta
      361 aatattgaaa taaagcaaag catttgattt ggtgcccggg agaagactcg aacttccaca
      421 cccgtgagga tactagcacc tgaagctagc gcgtctacca attccgccac ccgggctttg
      481 ctttatttgc tgttttgcgg tacagtgtcc tgccgcaaag aagtcgctat tatatagttc
      541 ataaagagaa tgtcaacagt ccaaatgaat aaaaatatta aatctttaaa tttacgggaa
      601 aaagacccgt ttttaagtcg tgaaaaacag cgttatgaac atcctttgcc cagtcgggaa
      661 tggataatcg aattgttgga gcgcaaaggt gtgccttcaa aaatcgaatc gcttgcgcgc
      721 gagctgtcga ttacggaaga cgagtatgtc tttttcgaac gccgtctgaa ggcgatggcg
      781 cgggacggtc aggttttaat caaccgccgg ggcgcggttt gcgcggcgga caaattggat
      841 ttggtcaaat gccgtgtcga ggcgcacaaa gacggtttcg gttttgccgt gccgctcacg
      901 cccgccaaag acggtgattt tgttttgtat gaacgccaga tgcgcggcat tatgcacggc
      961 gatattgtca ctgttcgtcc tgccggcatg gaccgtaggg gccgccgcga agggacagtt
     1021 ctggatattg tcgaacgcgc gcaaagcaaa gtggtcggcc gtttctatat ggataggggc
     1081 gtggcgattt tggagccgga agacaagcgt ctgaaccaaa gcatcgtatt ggaaccggac
     1141 ggcgtggcgc gtttcaaacc tgaatccggt caggtcatcg tcggcgaaat tgaggtttat
     1201 cctgagcaaa accggccggc agtggcaaaa atcatcgaag ttttgggcga ttatgccgac
     1261 agcggcatgg agattgaaat tgccgtgcgc aagcatcatt tgccgcacca attcagtgaa
     1321 gcgtgtgcca aagccgtgaa aaaaattccc gaccatgtac gcaaaagcga tttgaaaggc
     1381 cgcgtcgatt tgcgcgacct gcctttggta acgattgacg gcgaaacggc tcgagatttt
     1441 gacgatgcgg tgtttgccga aaaaatcgga cgcaattacc gtctggtcgt ggcgattgcc
     1501 gatgtcagcc attatgtccg ccccgatgac gctatcgaca cggacgctca ggaacgcagc
     1561 accagtgttt acttcccgcg ccgcgtgatt cccatgttgc cggaaaacct gtccaacggc
     1621 atctgctcgc tcaatcctaa tgtcgagcgt ttgtgtatgg tgtgcgatat ggttatcact
     1681 tacgcgggca atattaaaga ataccgcttc taccccgccg tgatgcgctc tcatgcccgc
     1741 ctgacctaca accaagtttg gaaatggctt tcaggcggca tcgggcatcc gttcaaagcc
     1801 caaatcgaca cgctttacaa actcttcaaa atccttcaga aaaagcgttt cgaacgcggg
     1861 gcggtggagt ttgacagcat cgaaacccaa atgcttttcg acgacaacgg taaaattgaa
     1921 aaaatcgtcc ccgttgtccg caacgatgcc cacaagctga ttgaagaatg tatgttggcg
     1981 gcaaacgttt gcgcagcgga ttttctgttg aaaaacaagc ataccgcatt gttccgcaac
     2041 catttggggc ccacgcccga aaaactcgcc gccttgcgcg agcagttcgg tctgttgggg
     2101 cttcaacttg gcggcggcga caacccgtcg ccgaaagact atgccgcgct tgccggacag
     2161 ttcaaaggca ggccggatgc cgaattgctg caagtcatga tgttgcgctc catgcaacag
     2221 gcggtttacg aaccgcattg cgacggacac tttggtcttg cctacgaagc atacgcccac
     2281 ttcacctcgc ccatccgccg ctatcccgac ctgaccgtac accgcgccat caaagccgtg
     2341 ttgaatcagc aaacctacac gccaaaaaaa agctggcagg ctttgggcgt gcatacctcg
     2401 ttctgtgagc gccgtgccga cgacgccagc cgcgacgtgg aaaactggct gaaaacctac
     2461 tatatgcgtg acaaagtcgg cgaaatattt gaaggcaaaa tttccggggt ggcaaatttt
     2521 ggaatatttg tcactttgga cgatatccat atcgacggtc tggtgcatat cagcgatttg
     2581 ggcgaagact atttcaactt ccgccccgaa atcatggcaa tcgaaggtga acgcagtggc
     2641 atccgtttca atatgggcga cagggttgcc attaaagtgg cccgtgccga tttggatgat
     2701 ggaaaaatcg attttgtcct gattgccgag gggagcggca gagggcggaa agttaaatca
     2761 tccgcgtctg ccaaaccggc agggacggcg gggaaaggga agccgaaaac cgccgccggg
     2821 aaaaaaacag cccgaggcgg caaagtaagg ggaaggggcg cgtctgccgc cgcagaatcg
     2881 gggaaaaagg caaagaaacc gattccgatt gaagtacaaa aggtaaaaaa acggaaaggc
     2941 aaatcataat gctgacgggg cggcttgggg agggcagggc agggcatact ttcccgaagc
     3001 cgccgcccga ttttttgata tggcggccgg cgcatcattt caggccaatc gaaacgcccg
     3061 tattgaaaga ttgcgtttat ttccaccgcc gttttaaagg ccggcggtat tcggcagact
     3121 gggcgcaaac ggcgttcaga cggcattttc attctttcgg cgtgtccgtc cgaattgctt
     3181 tgcccgtccg cgcaatcagc ccgtgcggct tgccttgaac ggacaaaaaa tgccgtctga
     3241 aacccgaaaa tcaggtttca gacggcattt tgccttgaaa aagctgttca aatcagcgat
     3301 ggtagttcgg tgcttctttg gtaatttgaa cgtcgtgaac gtgcgattcg ctcatacctg
     3361 cggaagtgat ttccacaaat tcggcttttt cgtgcatttc agcaatattg gcgcaaccca
     3421 aataccccat gctggagcgc agtccgccgg tcagttggtg gatgatattc acgatcgggc
     3481 ctttgtaagg cacgcggcct tcgatacctt cagggacgta tttgtcggtg ctgtcggttt
     3541 tgtcttggaa gtagcggtcg gcagaacctt ggctcatcgc gcccaaggaa cccataccgc
     3601 gataggattt gtacgagcgg ccttggtaga gttcgatttc gcccggcgct tcttccgtgc
     3661 ctgcaaacat accgccgagc atgacgctgt acgcgcctgc ggcgagggct ttggcgatgt
     3721 cgccggagaa gcggatgccg ccgtcggcaa tcagcggaac gcccgtgcct ttgagggctt
     3781 cggcaacgtt gtgaatggcg gtcagttgcg gcacgccgac acctgccacg atacgggtgg
     3841 tgcaaatcga tcccggaccg ataccgactt tgacggcatc cgcgccggcg gcgaccaaat
     3901 ccaatgcggc tttggcagtg gcgatgttgc cgccgatgac ttggatgtgc ggataggttt
     3961 ctttcaccca tttcacgcgg tcgatcacgc cttggctgtg cccgtgggca gtatcgacga
     4021 caatcacgtc cacgccggct tcgaccaagg ctttgacgcg ctcttcggta tcgccgccgg
     4081 tgccgactgc cgcaccgacg cgcagacggc cctcggagtc tttgttggca ttgggaaatt
     4141 cggtggtttt taaaatatct ttgacggtaa tcagaccttt gagttcgtct ttttcgttca
     4201 gaaccaaaac gcgttcgact ttatgcgtgt gcatcagttc gcgcgcttcg tctatgcttg
     4261 tgccttcggg gacggtaacc aagcgttcgc gcggggtcat aatggcggaa acgggcaaat
     4321 cgacgcggtt ttcaaaacgc aggtcgcggt tggttacgat gccgacgact ttgccgtttt
     4381 caacgacggg caggccggac attttgcgtt tgcgctgcgc acgcatttcc aagacttcgc
     4441 ggatgagcgt tgtcggtgca acggttacgg ggtctttgac cacgccgctt tcgtggcgtt
     4501 tcactttgga aacggcgcgc gcctgcattt cgggcggcat gtttttatgg atgatgccga
     4561 tgccgccttc ttgtgccatc gaaatggcga ggcgcgcctc ggtaacagtg tccatcgcgg
     4621 cggaaagcag ggggaggttg agtgtgattt cgcgggtgag cttggtttga agtttaacgt
     4681 ctcgcggcag cacggttgag tgtgcgggaa ccaacaaaac atcgtcgaaa gtataggctt
     4741 tttctacgat acgcataatg ctcggtcttt cagtttgtgc aagatgcacg gcattatagc
     4801 acgttaccgg cggcttgaca gtttatcagg tttaattttg gtcccctttg aatagcccgg
     4861 ttttcctttg ccgaccactg ttgctcccgt tctttcaatt tcaggaaaag cttttttcta
     4921 atttttggta agtggctcag ttattgaagc cctatatcgg gcggtaactt ccagcacaaa
     4981 aaaacggagt agttctttat ttattttttc ctttaatttt cagtatatta tcttaatatt
     5041 tcgagggtaa catatctgct aatctagtta cagccccata tattatagat tcaattgaaa
     5101 aataacagac tcaactgtac ctttcttata cctgatttct ttaaagtttt tcccattgtc
     5161 aaaactataa aaaagtctct cttcctcacc agaatatccc atttctaata ctaaacaatc
     5221 tatttcacaa ttaccccctc cactcatata tgtagtatca atagaaaata tattgcataa
     5281 ttccagagaa ttatatttat ctatatcaaa tagttgctct ctaaaatcta aaccattgtt
     5341 tccacctgca taatacttct cttcaaagga tgacttcaaa ctattaaaaa ctgtatttag
     5401 cgtaaaatat ccatatgggc atatcttagg atatatattc tctcctatta ttatctgaat
     5461 ttttccataa ataaaaccat tacattcaga gttttcttcc cataaaatcc caaattttct
     5521 tgttgagtcc ataataatat tcatataaat ccttatatta ataaattatt tacaatatcc
     5581 cccgctttca gacggcatac ggcgtggcgg cggaatgccg tctgaaggcg ggcgttatga
     5641 taattgttcc agcagcgtct gcttcaatga ggactggatt tttgggtttt tcaggtctag
     5701 gctaaaaacg gtaaaactgt cttcggcgcg ttcatccagt gtggagattt tggcatagcg
     5761 caggctgacg ttgtgggcga aaaagacttc cgccatatcg gcgagcagga aggggcggtt
     5821 gacggcggtg atttcgacgg agtaccagtc tggatagtct tcttcggggg tgatggtgat
     5881 gcccggtgca ataggcatat agcggctgcg gcggctgatg cggcggctgt ggtttgaggt
     5941 ttcggcaacg gtgtgtccgt ggataaagct gttgagttcg gcttcaagcg cgctttgaat
     6001 gtcggggtag tcttcggggg cgtgttgtga gggaatttgc acgatgaagg tgtcgaggat
     6061 gtagtcgtgt tcggtgataa aggcgcgggc ggcgaggata tcgaagccgt ggcggctgaa
     6121 aatgcggcag agacgggcaa acaggcgcgg gccgttgggc atgaaaacca tgacttgaaa
     6181 gctgtcgctt ttgggcagga tgcggctgcg gacgatgggg gtttcaaagt cgtgtacaag
     6241 gttggcggcg tgccacagga tttcgcggga ctggtggcgg gcgaagtagg cggaaccgag
     6301 cgcgttccaa agctttttct gctgtttttc ggggacggcg gcgcgggtga gtaagtcggc
     6361 ggcttcctgc cggcggcggc cgaagagggt gtgcgggttg ccgccgttgc ctgtaaggta
     6421 gcgtccggcg gcatggaaga ggctttccag caggctggcg cgccatgcgt tccacagctt
     6481 gggattggtg ccgcgtatgt cggaaatggt cagaaggtag agcgcgctga ggcgttcgtg
     6541 ggtttggacg cgtttgcaga aggcatcgag tacgctgggg tcttggatgt cttctttttg
     6601 ggcgacggtg gacataagaa gatggttttc aaccagccag gcgagcaggt cgctttcttc
     6661 ttcggtcagg aagtggtcgg cggcaaattg gcgcgcgtct gcgatgcctt gtatggcatg
     6721 gtcgccgccg cgtcctttgg cgatgtcatg gaaaaaggcg gcaaggtaga ggatgtcttg
     6781 tttttcaaag gactgcatca gtgcagaggc gtagggcagc tcgtggctgt gcatatccaa
     6841 ggcaaggcgg cggacgttgc ggacgacggt gaggatgtgg tcgtccacgg gatagatgtg
     6901 gaacaggtcg tgctggagca ggccgacgat tttttcccac gcgggcaggt agcgtcccaa
     6961 tacgccgtag aggttgagga agcgcagggt ttgggtcagt ccgttgccgt tgcggaaaaa
     7021 gccggcgaag cggtggcggt tttcagaatt ttggtagaag ctgcggttga ttttgcgcgt
     7081 cgcgccccac catgcgcgca gggtttgcgg ttcgagcgcg gtaatatcgt tgcgctgctg
     7141 catgatttcg acgattttga aaatgtgttc gggccgtctg aaaaaaatat cggtgtgccg
     7201 cgcggcgatt tggttgttga cttggatgta gtcgtcgtca atccgcaggg taacgcgcaa
     7261 cggggtggag gaaacgcggc tttgcagcat aggcgtgagg atgccgccca gttgtttgac
     7321 ggttttaatc gcgcggtaaa acacgcgcat cagttcttcg ctttggcggc ggaggttcaa
     7381 gccttcataa cccatgcttt cggcgacttg cggctgcaaa tcgaacagca ggcggtcttc
     7441 ggcgcgcttg gcgtttaaat gcagatggat gcggatgtgg gcgaggcggc ggtagccgtg
     7501 cgaaagcata ccggcttcgg cacgcgtcaa aatccgctgt ttgagcaggt cgggcaggtc
     7561 ggtcgccaag ccttgcgcct tcgctatcca aagcagggtg tggatatcgc gcagaccgcc
     7621 cggacagctt ttgatattcg gctccaatac tgcccccgaa ccttgcgatt tggcgtggcg
     7681 gtgttccatc tccaccagtt ttgcctcgac aaacgccgcc acattgcgct gcgcgttcat
     7741 tttttccgct agtttatccg ccgtttggcg gttgccaaac aaaaacctag cctctaaaaa
     7801 cgctgtgtcc cccgtaatat cattgcgcac gctttcacat agttcatcaa cgctgccgct
     7861 ttttacagac ggcatcagtt tgcagtccca cagggtttgg acaaaccggg caatctgttc
     7921 ctgaatgccg tctgaaagcg gggcagggga gacaaccgcc aaatccacat ccgaacaggg
     7981 atacagttcg ccgcgtccga agccgcctac cgccatcagg cataacgcgc tgttttgaaa
     8041 atactctgcc cacaatgccg ccagtaaggt ttcgagtgcc gccgtatatt ccctgaaaaa
     8101 taccgacacg cggttggctt tcaaataatg cgcttcggcg gcatcgcgct gctgtttgaa
     8161 ggtttccagt gctgaagaca ggtttgcagg catttttatt ctttcgattg gcgggaaaaa
     8221 gggaggcgga tggttcggcg gtcaaatacc gctttcagac ggcatttgtc gggtatcggc
     8281 ggaattggtc atcagcttca accgttcctg cggcggcagc agcaactgat acgccgccac
     8341 gcccaaagcc gccgccattt tttcgatatt cgacagggcg atgttccagc gtttgcgctc
     8401 gactgccgac acataagtcc tgtccaaacc gcattgccgc gccaattctt cttgcgacca
     8461 acccttgttc acgcggaaaa gccgcatatt gtatgccaat accgcccgca aatcctgttc
     8521 gtcaggcagt tcggcaggca gagtcaattt gttgcccatc atttgcttcc agataaatgg
     8581 ttaaagttaa gcatttgctg ttacggattt tacttcacat aaaagccaaa aaaaaggggt
     8641 ggcggcagcc acacccaaac acacacacat caagaggaaa gagataaaat cagacagatt
     8701 ggaaaacagc aaacaagcac cctgaatttc atatcggtat tatcaaacaa aaacccttat
     8761 caggtattga ttcaaatcaa tttagatttt attcgcaaac cgaaaaagaa aaacggcata
     8821 tccgtttata cggatatgcc gtctgatggt gcgggggact gctgtatttg gcagtagttt
     8881 gtgttttagt cctcttgcgc cgctgtttgc aggtagttgg gcagaccgat tttgccgata
     8941 agctcttgct gggtctccag ccagtctata tgttcttcgt tggtgtcttt ttgtttttcc
     9001 aacaaatcgc ggctgacgta atcctgttgc gcttctgctg tggcgatggc ggcaagcagg
     9061 gcttcgtgtt tttcctgttc tttggtcaaa tcgcaggcga tgatttcttc ggtggactcg
     9121 ccaatcagaa gcttgcccag ttcttgcagg ttcggcagac cttcgaggaa cagaatgcgc
     9181 tcgatcaaat cgtcggcagc tttcatttca acgatggact gtttgaagaa atgttcgccc
     9241 agttcttcaa agccccagtt tttcaaaata cgggcgtgaa ggaaatattg gttgatggtt
     9301 accagcagca agcctaagtt tttgttcagc tcgcggataa ccaaacggtc gcctttcata
     9361 cggactcctt ttattccgaa tacggattac agttggcttt ggtagtagtt gccttcgcca
     9421 attaactcga tcaggcgaag ctgctgttcc agccagtggg cgtggtcttc ttcggtatct
     9481 ttcagttggg caaccatcag gtcgcgcgta acatagtctt gagcctcttc gcacagtttg
     9541 atgccttttt tcaaagcgtc gcgcacttcg tattcggttt gcaggtcggc tttgaggcag
     9601 gaaaccacgt ccgtgccgat attcagctcg gcgcgcgcca ttttcggcgt accgcccagc
     9661 atcaggatgc gtcggatgaa gtcttcggcg tgtgtggttt cttcttccat ctcgtgattg
     9721 agacgttcaa aaagtttggt gtagccccat tcggagtaga ggcgggagtg gataaagtat
     9781 tggtcgcgtg ccgccagctc gccagacagc aattcgttca tataatcaac aacagcttga
     9841 ttgccttgca taatatctct ctttccgtaa cttggttttc ggtatgtctg aacagcatct
     9901 gtgtcgctat tcgggatgcg tgcattgtat gcaaaagctg tcggcatgac aaattttcta
     9961 tttaaaatac aaacaattat caaaagaaat agggcgtatt ccccgatgcc ttccaaatca
    10021 gtatgctgtt tcttatcggt tttttgtgct gttaaaacta agaatccata tcatccataa
    10081 agaacattta cacttgttaa ccatagcgaa aagcaaatac tgtacggttt tttatcaaaa
    10141 tttataatga atcgttctca ttaaccgaca gattcttttg agattatcgg attttgggaa
    10201 taaattatgc cgtctgaggg gctttcagac ggcatagaca attaaagctg attaaacaac
    10261 gcggcagaca gcagcgtagg aaattatttt ttacttcgga acggtatttt gggtttaatg
    10321 atgcccgcaa ccgccgtggc tttcgcgtaa ggcttcggca gcctgttcgt cggcgtggta
    10381 gctggaacgt accatcgcgc cgatggcggc attgctgaag cccagttcgt atgcttcttt
    10441 ttcaaagatt ttgaactgct cgggcgtaac gtagcgcagg acgggcaggt gtccgtctga
    10501 aggctggagg tactgtccga tggtaatcat ttcgatattg tgcgcccgca tatcgcgcat
    10561 aatttcacgc acgtcttcgt ctgtttcgcc caagccgacc atgatgccgg atttggtcgg
    10621 gatatgcggc atcatttctt tataacgttt taataagtct aaagaatgtt gataattagc
    10681 accgggacgg gcttttctgt acaggctcgg atgggtttct aggttgtggt tcatcacgtc
    10741 gggcggggtt tcggcaagga ttttgagtgc gatgtccaag cgtcctcgga agtcggggac
    10801 gaggatttcg attttggtgt tcgggctggt ttcgcggatg gctttgatgc agtcggcgaa
    10861 atgctgtgcg ccgccgtcgc gcaggtcgtc gcggtcgacg gaggtgatga cgacgtaacg
    10921 caggttcatg gctttgacgg attcggcgag gtttttcggt tcgtcggggt cgagcatatt
    10981 cgggcggccg tgtcccacgt cgcagaacgg gcagcggcgg gtgcagatgt cgcccataat
    11041 catgaaggtc gccgtgcctt tgctgaagca ttcgccgatg ttggggcagg aggcttcctc
    11101 gcaaacggtg tgcatttttt gttcgcgcaa aatgtctttg atttcaaaga atttgcgcga
    11161 tggaagtttg gcgcgtatcc attcgggctt ttttaatttt tcctgaaggg ggacgacttt
    11221 gatggggatg cgggcggttt tgtccgcgcc tctgagtttg atgccgcgtt tggggtcgtc
    11281 ggttttgatt tcactcattg ttgtctgctt tcggtgtgaa ttgtgtttca aggtgtgcgg
    11341 tgagtttggc ggcgacttcg tccggcgtgg ggcagggttg gacaaaattc gcgatttgcg
    11401 tcatttccat accggcgtag ccgcaggggt tgatgtgggt aaacgggctt aaatccatat
    11461 tgacgttgag cgcaagcccg tgatagacgg agccgttttt gatacgcagc cccagtgagg
    11521 cgattttgcg ttctccgaca taaacgccgg ggcgtttggg gtctgccgcc gcttcgatgc
    11581 cgtattctgc caatgtggcg atgatgctgt tttcaagcgc ggaaacgatg tttctaacac
    11641 tggttttgcg ccgtttgaaa tcaatcatcg tataaacgac caattgcccg ggcccgtgat
    11701 aggtaatctg cccgccccgg tcgatttgga cgacgggaat gtcgtcgcga atcagcaggt
    11761 gttcgggttt tcccgccagt ccttgtgtga acacgggcgg gtgttcgacg acccacagtt
    11821 cgtcttcggt gtcggcattc cgtccggcat taaaggtttt catcgcttca aaagtcggca
    11881 gatattcgac caaacctttg tgtatgattt tcatctcaaa gtaccacttt gaccagttcg
    11941 tgcgaagtca gcgcacggta gatgttgtcc aattgttctt ggttttcaac ctttacctgt
    12001 acggtggcgc cagtatagtt gcctttgctg ctcggacgcg tggtgatgtg gtgcgcctgc
    12061 gtgtcggggg cgtggaggcg gacggtgtct aaaaccgcct gctcgaactc gggatgcacc
    12121 gcgcccatta ctttcaatgg gaaggtgcag ggaaattcga tgagggatgt tttgtttttt
    12181 tgttcggtca tgatgtgctg ccttgtcgtg tacggtatgc cgtctgaagg cgggtttgcc
    12241 tttcagacgg catcggatgt gcgttatttt agcctaaacc gcgataacag gctatcggga
    12301 aggcggaggc ttttttgacg gcgcggcggt tctgctatac tggcgcacaa tattattttt
    12361 agaagggtgg tttttatgta tcggaggaaa gggcggggca tcaagccgtg gatgggtgcc
    12421 tgtgcggcgt ttgccgcctt ggtctggctg gttttcgcgc tcggcgatac tttgactccg
    12481 tttgcggttg cggcggtgct ggcgtatgta ttggaccctt tggtcgaatg gttgcagaaa
    12541 aagggtttga accgtgcatc cgcttcgatg tctgtgatgg tgttttcctt gattttgttg
    12601 ttggcattat tgttgattat tgtccctatg ctggtcgggc agttcaacaa tttggcatcg
    12661 cgcctgcccc aattaatcgg ttttatgcag aacacgctgc tgccgtggtt gaaaaataca
    12721 atcggcggat atgtggaaat cgatcaggca tctattattg cgtggcttca ggcgcatacg
    12781 ggcgagttga gcaacgcgct taaggcatgg tttcccgttt tgatgaggca gggcggcaat
    12841 attgtcagca gtatcggcaa cctgctgctg cttcccttgc tgctttacta tttcctgctg
    12901 gattggcagc ggtggtcgtg cggcattgcc aaactggttc cgaggcgttt tgccggtgct
    12961 tatacgcgca ttacaggcaa tttgaacgag gtattgggcg aatttttgcg cgggcagctt
    13021 ctggtgatgc tgattatggg tttggtttac ggcttggggt tggtgctggt cgggctggat
    13081 tcggggtttg caatcggtat ggttgccggt attttggttt ttgttcccta tttgggcgcg
    13141 tttacaggac tgctgctggc aaccgtcgcc gccttgctcc agttcggttc gtggaacggc
    13201 atcttggctg tttgggcggt ttttgccgta ggacagtttc tcgaaagttt tttcattacg
    13261 ccgaaaatcg tgggagaccg tatcggcctg tcgccgtttt gggttatctt ttcgctgatg
    13321 gcgttcgggc agctgatggg ctttgtcgga atgttggccg gattgccttt ggccgccgta
    13381 accttggtct tgcttcgcga gggcgtgcag aaatattttg ccggcagttt ttaccggggc
    13441 aggtaggcgg ttccgaaaca tatttgaagc ggaatacaac cttgcccggg tttaaataaa
    13501 aatgccgtct gaaccccgaa aactggactt cagacggcat tttcatcacg gcttatttgg
    13561 cggttttgct gctgtcgata attttcatac cggcagaaat caggctgccg atgtcggcaa
    13621 cattggcggg cataatcagc gtattgcttt ctttggcaag attgttgaac gcagcgacgt
    13681 attgttccgc aatcttcaga ttgaccgcat ccgcaccgcc ttgggtttga agggcggcgg
    13741 caatttgacg gatggcttcg gcattggctt cggcaacaag gcgcaaggat tccgcttcac
    13801 ctttggcgcg gttgatgcgg gcgattttct cggcatttga cgcattgacc gcagcctgag
    13861 cctcgccttc ggattgttgg atttcggctt cgcgctgacc gctggcaagg ttgatttgtt
    13921 cgattttacg gccttcggat tcggcgatac gggcgcgttt ttcgcgttcg gcggtaattt
    13981 gcgcctgcat tgagcgaagg atttcttgag gcggcaccaa gtctttaatt tcataacgca
    14041 aaaccttcac accccaagct ccggcggctt catcgagggc ggagacgacg atactgttga
    14101 tttcgtcgcg ttcttcaaac gttttgtcca actccatacg cccgataacg gaacgcagcg
    14161 tcgtttgggc aagctgggta atcgccataa tgtagttgct cgaaccgtat gaggcgagtt
    14221 tggggtcggt tacttggaaa tagatgatgc cgtcaaccgt cagctgcgta ttgtcgcgcg
    14281 tgatgcagac ctggctgggt acgtctaaag ggatttcttt cagcgaatgg cggtaggcga
    14341 cgcggtcgat aaagggaatc aaaatattca aaccggccgt cagggcgcga tggaaacgcc
    14401 ccagcctttc gacaacgtgg acttcctgct gtgggatgac gacaaaggat ttgaagccga
    14461 aaacggcaac ggctgccaac aagataatga aaaattccat aattcctccg agtgttaagg
    14521 ttttgcgatg ataagaaggt tgccttcctt gcggacgatt agggcgcgcg ttcctggttc
    14581 aagctcttct tgccccgtat tttgcgcctg ccagtgcgta ccgcgataaa aaacttcgta
    14641 acggttgccg cctgtgtgtc ggaggatttc gacatattgt ccggcatcca aatcctgata
    14701 tgaatccgtt tcaacttttc ccacggcggt tttggcgtgt acgaaccaaa tacccagcgc
    14761 ggaaagcaga gcagcggtca agacggcggc aggcgtgctg ccggtcagtc cgtagataat
    14821 gcccgaaccc gccaaagctg cactgacaac cagaagataa accgttcccg tcaataattc
    14881 gatgattaag acggcaacag cagcaacaaa ccatacagtc atacatttcc ccacaaagcg
    14941 cgtcgtttga caaaatcacg cgatatcagc agtatagccg aatttgaaag gatagggcag
    15001 atatggatac ttggcacgat gcactgggca acgaaaaacg gcaaccgtat tttcaggaaa
    15061 ttttaaatgc agtcaggcag gaacgtttgt cgggacaaat catctatccg ccggcggcgg
    15121 atgtgttcaa cgcattccgc ctgacagcgt tcgaccgggt caaagccgtc attctcggac
    15181 aagatccgta tcacggggca gggcaggcgc acggtttggc attttccgtc cggcagggtg
    15241 tccgcatacc gccgtcttta ctcaatatct acaaagaatt ggaaaccgac atcaacggtt
    15301 tttccattcc cgatcacggc tgcctgacgg cttgggcgga gcagggcgta ttgcttctga
    15361 acacggtttt gacggtgcgt gcaggacagg cgcattcgca cgccctttta ggctgggaac
    15421 gctttaccga taccgttatc aggcagcttg cgacacaccg caagcacctt gtcttcatgt
    15481 tgtggggtgg gtatgcacaa caaaaaggga ggctgataga cagtcaaaat catttgatat
    15541 tgaccgcacc gcatccatct cctctgtcgg catatcgcgg tttttttggc tgccgccatt
    15601 tttcacaggc aaacagctat ttgagccggc acggtatcga tccgataaac tggaagctgt
    15661 gaatgccgat atagccgttg ccgccggcgt gttaaaatcg cgtttgattt gtaatttcca
    15721 tttattaggc aaaaccttat ggcaatcaaa aaaaatcagc tttatgcatc actttgggcg
    15781 ggctgcgacg agttgcgcgg cggtatggat gcgagccttt ataaagaact atgtgcttac
    15841 gctattgttt ttaaaatatg tttctgataa gcataagtac ggcggcggca tgattgagct
    15901 gcacgccggt acgacttttg acgacatcgt caaactcaaa aacaccgccg acatcggcga
    15961 ccgcctgaat aagattatcg cccaaattgc cgaagccaac gacttaaaag gcgtgatcga
    16021 cgttaccgac ttcaacgacg aagacaaact gggtaaaggt aaggagatga tcgaccgttt
    16081 gagcaggctt gtcggcattt ttaaaaagct caacctttct tccaaccaag ccgaagacga
    16141 cgatttgtta ggtgatgcct acgaatacct gatgcgccat tttgcgaccg agtcaggcaa
    16201 atccaaaggg cagttttaca cgcctgccga agtctcccgc attatggcga agattatcgg
    16261 aatcagcgca gattgcccgt ccagcaccag cgtttatgac ccgacctgcg gctcgggttc
    16321 gctgttgctc aaagccgccg cccaagccgg cagccaaatc agcctttacg gtcaggaaaa
    16381 agatgtggca accgcgtccc ttgcccgtat gaatatgatt ttgcacaaca acgaaaccgc
    16441 cgaaatcaac accgggaaca ccttgtccga ttcgtctttc cgtgatgaaa acgacgggct
    16501 taagaccttc gattttgccg ttgccaatcc gccttatccc gcccgaaaaa aacggcgatt
    16561 acgccttttt gctgcatctg ctcaaaagcc tgaaaccaag cggcaaaggt gcgattattc
    16621 ttccgcacgg tgtgctgttt cgcggcaatg ccgaagcgcg tattcgcacg gaattgctta
    16681 accttgacct tattaaaggc attatcgggc tgcctgccaa cctgttttac ggcacgggca
    16741 ttcctgcctg catcatcgtc atcgacaaag aacacgccca aaccgcccaa tttgccgaag
    16801 agggaacaaa ccaagttatc agcggcggca gcgtgtttat gattgacgca tcgcgcggct
    16861 tcattaaaga cggcaacaaa aaccgtctgc gtgagcaaga cattcacaaa atcatcgaca
    16921 ctttcacaaa cctcgttaca gccgtatggt gcatttaagc gaaatcgcag cacaagatta
    16981 caaccttaat ctgcctcgct atatcgacag cggagaagtc gaagacctgc aaaatctcgc
    17041 cgcctatcta tctttatggc ggcatacctg cgcacgatat ggacgcattg gaagcctatt
    17101 ggcaagtttt aggccgtatg aaaaacgagt tgtttgccga acacgatggc cactttacca
    17161 ctatacaacg gaatcgattg caaatctttc ccactctcaa cagcttaaaa tcctgcggga
    17221 ttggtgtgga atttagggct aatctagtac agccccaaat ttaatccact ataaaatcga
    17281 aagcagccaa atcaaagccc atatattggc gcaccccgat tacgccgcct tcaaagccgg
    17341 acacctggca aagtttgccg cgtggcacac tcaaaacgac cttgccgcca tccaaccggg
    17401 caggcttatc cggaaatgga gcgaaagcct gctggacgcg ttcaaacccg gcagcctgat
    17461 tgaagaatac gatttctacc aaatcctgac ggactactgg gcggaaaccc tgcaagacga
    17521 tgtttatctc atcgcccaag atggctggaa ggcggttaaa aacctggccg aaatcaccaa
    17581 agaaagcgat gaagccgcga acctgaccgt cgtctttgag gaaaccgaaa ccgacaaaaa
    17641 aggcaaagcc aaaaccaagc gcatcagcaa aaaataccgc agcgaagtca tcgcccccga
    17701 gctggttgcc cgccgctact tttcagacgg catcgccaag ctggaagaaa aacaaagcga
    17761 gctggaacgc ctaagccaag aattggaaaa ccacatagaa gaacacggcg gcgaagaggg
    17821 tgcgctgaac gacgtattgg atgcaaaagg caaactttcc gccaaacttc tgaaaaccgc
    17881 attggaagaa agcggcatag aagaaggcga acgggctgtt ttacaaacca cccaaacact
    17941 gatgacgcag gaaaaagccg cgaaagacgc agtcaaaacc caaatcgaag ccctgaacct
    18001 tgccgtattc aaacaattcg gccgactttc cgaagccgaa atcaagcagc ttgccgttca
    18061 agacaaatgg cttgccgatt tacaaagccg aatcgaaaat cgcttggaaa acagtattca
    18121 gcagcttatc agctgcttga acacgctgga agaccgctac cgcagcccga tggccgagct
    18181 tgcccgagaa gtggaaaagt ggcaaagcaa agtcaatgcc caccttgaaa atatgggttt
    18241 tggaggctga aatggcggca cagacaggct ataaggcgag cgggttttga gacctttgca
    18301 aaattcccca aaatccccta aattcccacc aagacattta ggggatcgcg gttcgggtgt
    18361 ccgcaccgct taatacgtcg tcgtccacga actgacccat ttgctcgaac gccatcgcaa
    18421 cgcccgtttt atggcgcata tggacaactt tctcccaaac tggcaaagca tcaaacaaca
    18481 gcttaatgcc ttggagttat ttgcacaaat atataattta acataatata cattatgcga
    18541 actatcggaa acagttgtac gtgtccctgt ggtctttcca agtaggaaaa ttaaagtatg
    18601 gacaatgcgg ctgaatgtat agcccggagc atccgcgtat ccgaaggtac tgaacgatat
    18661 gccctgcatc cctttcgatg cttccatcca gccggtattg ggaaatacgt tgaccttcaa
    18721 tataatatac aggcagccga cacacaggat atgccgctgc tttttcatca tttcttctgt
    18781 caaatccttg gaacggtcga tttgaaaaca cggctttaca tacgccccag ttccctttgc
    18841 agggcttgaa tattttgctg cctgtccaat acattgcttt gtaatgcatt tatttcttga
    18901 tggttgatgt tgccgccctt tgccagacgt gcttgtgata acattttttg ggcttcaacc
    18961 aatgctttgc gttcgttgct caattctgtt tcgagaatgg agcgtctgct gttgtttgag
    19021 ggtgcttgtt gcggcggcgg cgtattggat tttgccggct tggatactgt tttgaccggg
    19081 gctttatatt tgacaacctg tccgccgttt gacggtgatg ataccggttc gggcgtttgg
    19141 ggtggaatat agcgttcgct gctgtagttg ccgattgggg gcaaatcggt tgagtggcag
    19201 cttttagacg gcttggtggt gtaaacggtt tctccgttga ttgtgcaggt gtagattttg
    19261 gccgcattcg cacccaatgg gcttgaaatc agggaaaagt tgattaggat taaggggagt
    19321 tttgatttca taatgtgctt gatgctcgga taattatttg attttttggt attttttgta
    19381 atgctttctt ggcgttttac gggcttttct tttcggttgt ttgcttttga ttttctttcg
    19441 gagctttgga agcctgcctg ttctttttcc cggcaggttt tttgtttttc tttttacttt
    19501 ctatgtgtat tttggcttct taactgagtt ttttaatttt caggcggtat ccgcctccct
    19561 gatggctgct gattttaggt aaatccgcat cggcgcacga tcctgctggg gctgatgtat
    19621 atacgctttt gacatttgag ttgcaatggt atacggaggc ttgcgccgcc gcgccggaaa
    19681 gggacaggag acccaaggcg gcaaaaagtc tgatgtttat accggtaaaa ggcggtgata
    19741 acgcccgaat tataccgtta ttggcaggca aagataagca ccctgcccgc gcttctttat
    19801 cgctcggcaa actgtttctc gatttcagtt tcaatccgtt ccaattcttc ttgacaagcc
    19861 agccaaacct cttcgatttg ggcaagttgt gttttgactt ttgccagctg ggataaggta
    19921 ttttgcaatt tttctttgtt tgcttcaagg taagcgtctt cttgtgctaa aaatgcttca
    19981 catgccgtct gaatttcgga aagctgcgcc atttcttttt cggcacggtc tattttctgc
    20041 tgtatcggct tgccgcgtcg ggctttttcc tgacggattt gcgcttcgat gcgcttggtg
    20101 tctttgcggc tttggctttg tgcggatgct gcgggtgcgg cggcgttttc ctgtgccaaa
    20161 cgccattggc ggtaatcgtt caaatcgccg tcgaagttct tcagacggcc tttgtcgatc
    20221 aggaggaagc tgtcggtagt cgcttcaagc aggctgcggt cgtgcgatac gatgattaag
    20281 gcgccttgga aactttgcag agcgagcgtc agggcgtggc gcatatccaa gtccaaatgg
    20341 ttggtcggtt cgtcaagcag caacaggttc ggcttttgcc agataatcat ggcaagggca
    20401 aggcgggctt tttctccgcc ggaaaaaggt tcggttttct gtaacgccat atcgccaaca
    20461 aaattgaagc cgccgaggaa atttcggatt tcttgttcgc gtacttcggg agaaagctgc
    20521 tgaatatgcc aaacagggtt ttggtcggcg cggatggtat cgagttggtg ttgggcaaaa
    20581 tagccgatat tgagtttttc ggaacggacg atgctgccgg agagcaaatc gattttgccc
    20641 gccaaggctt tgataaatgt agatttaccg ctgccgttga cacccaataa accatagcgc
    20701 gcgccgcttt ccagcgacag ggtaatgtcg tgcaaaacag ttttgccttc gtaacccaaa
    20761 tctgcgtgtt ccagcttcag caaaggattg ggcagatgct cgggattgta aaactcaaag
    20821 gaaaactcgc tgtccaaatg cgcgggagcg atgcgttcga gctttgccaa agccttcatg
    20881 cggctttgcg cctgaacggc tttggtggct ttggctttga agcggtcgat aaaggattgc
    20941 agatgtttga tttgcgcctg ctgtttgaca taggcggctt gttgctgcgc gagacgctgc
    21001 gcgcgttcgt tttggtaaaa atcgtaattg ccgccgtatt gcgtgagctt ttgctgggac
    21061 aattcgatgg tttgggtggt tgccgcattg aggaaatcgc ggtcgtggga aatgatgatt
    21121 tgcgtgcagg gtaaagaggc aaggtggttt tccagccaca agacggtttc taaatccaag
    21181 tggttggtcg gttcgtcaag caagagcaaa tcggcgcggc aaatcagggc ttgcgcaaga
    21241 ttcaggcgca tacgccagcc gccggaaaag gatttgacgg gacggctgtg ttcttcttgc
    21301 gaaaaaccca gcccgttcaa caatttcgct gcacgggccg gcgcggtata agcgtcgatt
    21361 tcttccaatt tggcgtgata ttccgcctgt ttcataccgt cgttttgcgc ttccgcctgc
    21421 ctcaatgccg tctgaagggt ctgtaactcg gcatcgccct gcaaaacgta atctaaagcg
    21481 gaaatatcca aatcgggcgt ttcttgggaa acggaagcga gccgccagtt tttcggaatc
    21541 gagacatcgc cgccgtcctg agtgatttca cccttgatta aggcaaacag gctcgatttg
    21601 cccgtcccgt ttttgccgat caaaccgacg cgctgaccgg gattgacggt agcgttggct
    21661 ttgtcgagca ggactttcaa accgcgttgc agggtgaggt ttttgatttc aatcataacg
    21721 gaaacatcgt cgggcgggaa aagcccgtat tttacctgaa aatcagtgcc gatgccgtct
    21781 gaaatgggaa atttacggct gaagccaagc ccaagccctg cgcccttccg agtgcaggaa
    21841 aaccaatgtc ctgaatgccg aatcggtatt catgcattcc acgctgattc cgattcggga
    21901 aaaatccgcc atgattttgg gatggataaa ctcctgagcc gcgcccgtcc cgataatcaa
    21961 tatttccgga tagtcaacag gtttgatgtc ggacaacagg ttttccggag tcagatcgga
    22021 caaggttcgg cattgcgaca ggcagaccga atccttatgt acaagcacgg gtttatggaa
    22081 actttgaccc gccaatcgga tttcacccga ccggtggcca tactctgtaa aatgtccgtc
    22141 tatcggattt tcttcaaaca acattttttt accccgtcgc cgcatcatct acaccgaaag
    22201 gggtgcaaaa tcaggcgaat tcatgtagga ttggcagatt tcatctgacc cgcctgccga
    22261 tttcagacgg catttgattc aaagtgcggc acaattatat cggcagcgga tattttcgtc
    22321 tttcaatatt tacatttcag tcggcttaca aggagacaca atgaagccag taaacatcgg
    22381 tcttttaggt ttgggtacgg tcggcggcgg tacggctgcc gtgttgcggg acaacgcgga
    22441 ggaaatttcc cgtcgcttgg ggcgcgaaat ccgtatttct gccgtgtgcg atttgagtga
    22501 agaaaaagcc cgacaaacct gcccgtccgc agcctttgtc aaagatccgt tcgaactggt
    22561 cgcacgtgaa gacgtcgatg tcgtcgtcga attgttcggc ggtaccggca ttgccaaaga
    22621 tgcggtgttg aaagccattg aaaacggcaa acacatcgtt accgccaaca aaaaactgct
    22681 cgccgaatac ggcaacgaaa tcttcccgct ggcggaaaaa caaaacgtca tcgtccaatt
    22741 tgaagcggca gtagcgggcg gtatcccaat catcaaagcc ctgcgcgaag gtttggcggc
    22801 aaacaggatt aaatccatcg ccggcattat taacggcacc agcaacttca tcctctccga
    22861 aatgcgcgaa aaaggcagcg cgtttgccga tgtactgaaa gaagcgcagg cattgggtta
    22921 tgccgaagcc gatccgacct tcgacatcga aggcaacgat gcgggccata aaatcaccat
    22981 catgagcgca ctggcattcg gcacgccgat gaacttttcc gcctgctacc tcgaaggcat
    23041 cagcaaactc gacagccgcg acatcaaata cgccgaagaa cttggctatc gcatcaaact
    23101 gttgggcatt acccgcaaaa ccggcaaagg catcgagctg cgcgtccacc ctaccctgat
    23161 tcccgaaagc cgcctcttgg caaacgtcaa cggcgtgatg aacgccgtgc gcgtcaacgc
    23221 cgatatggtt ggcgaaacct tatattacgg cgcgggcgcg ggcgcattgc cgaccgcttc
    23281 cgccgtggtt gccgatatca tcgacatcgc ccgcctggtt gaagccgata ccgcccaccg
    23341 cgtaccgcat ctggcgttcc aacccgcgca agtccaagcg caaaccatcc tgcctatgga
    23401 cgaaattacc agcagctact acctgcgcgt ccaagccaaa gacgaaccgg gcacgctggg
    23461 gcaaatcgcc gcgctgttgg cacaagaaaa cgtgtccatc gaagcactga ttcaaaaagg
    23521 cgtgattgat cagaccactg ccgaaatcgt gattctgacc cacagcacgg tcgaaaaaca
    23581 catcaagtcg gcaatcgcag ccatcgaagc actggattgc gtggaaaaac cgattaccat
    23641 gatccgcatg gaaagcctgc atgactgagc cgaaacacga aatgccgacg gaagagcagg
    23701 ttgccgcgcg caaaaaagca aaagccaaaa tccgcaccat ccgcatttgg gcgtgggtca
    23761 ttttggcgtt gctcgcttta accgccctgc tctcccaatg cgcgatgtcc aaaccgcagg
    23821 caaaacagaa aattgtcgag tcttgcgtga agaatattcc gtttgccgaa aaatggcaaa
    23881 acgatttgcg ggcccgcggt ttagattcaa acaatacccg cctcgccgtc gactactgca
    23941 aatgtatgtg ggagcagcct ttggacagat tgagcgagaa acagattaga tccttcggca
    24001 aactcggcgc acaagaacag cttgacctgc tcggcggcgc aaatgccttt gaagcacgtg
    24061 acaagcagtg tgttgccgat ttgaaatcag aataatgtgg accgataaaa aagccgattc
    24121 tttaaagaat cggctttttt cataaaaaac ggcttacagt gcgtctttca atgctttacc
    24181 tgcacggaat ttaggcgttt tggcggcggc aatggtcaga ggctcgccgg ttttggggtt
    24241 gcggccttgg cgttccgcac gttcgcccac gtagaaagta ccgaaaccga ccaaagtaac
    24301 ggtgtcgcct tgtttcaggg cggtggttac tgcattggta gtggcatcca aagctttttg
    24361 tgcggcggct ttggaaatgt cggcttcttg agcaatcgct tcgatcaatt cagacttgtt
    24421 cacaatcagt cccttcctgt cttaaaaaat gatgaaatgc ccgaatactc ggggtttgta
    24481 ctgcttgagc aactttcgct ttatagcaat tctgaaattg ccgtgtcaag caaaaaatac
    24541 ggaatcaccc tatttgacag gctttcagga cgaaaccgca tttttacaac acatttcctg
    24601 cgtttcaatg tttggttgcc ctgctgcggg gttttggttt tgaagcggat tccgccgccg
    24661 cttccgcacc agaaggttct gcccaaggct caggctggct ttccaaaccc agagccaata
    24721 cctcgtctat ccatttgacc ggatggatgg tcaggccggt tttcacgttt tcagggattt
    24781 cttccaagtc tttgaggttg tcttttggaa ttaaaacgtg tttgatgccg ccgcgaagcg
    24841 cagccaacag tttttccttc agaccgccaa tcggcaggac ttcgccgcgc agggtaattt
    24901 cgcccgtcat ggcaacatcg gcgcgcaccg ggattttggt gaatgcagac accatcgcca
    24961 aagtcatggc aatgcccgca ctcggaccgt ctttcggcgt cgcaccttcg ggaacatgga
    25021 catggatgtc ttttttctcg taaaaatcag gagccaagcc cactgattcc gcgcgggagc
    25081 ggacaaccga ccacgctgcg gacacggatt ccttcatcac atcgcccaac tggccggtgc
    25141 actgaatcac gcccttaccc ggcaatgctg cggcttcgac ggtcagcaat tcgccgccga
    25201 cttccgtcca cgccaaaccg gttacttgtc cgatacggtt ttcgctttcg gcaacgccgt
    25261 aatcgaagcg gcgcacaccc aaatagtcgt gcagattttt ctcatttact ttaaccgccc
    25321 tgggcttggc ttttccggtt tttttggttt ttgagtcttc gtccaaggta atctgcatca
    25381 ccaccttgcg gcagattttg gcaatctcgc ggtcgagcga acgcacgccc gcctcgcggg
    25441 tgtagtaacg gataatatcg cgcaccgcgc tttcttcgat tgccaattcc ccttctttta
    25501 cgccgttgcg cttcatttgc ttcggcacga ggtactgcat cgcgatattg attttttcgt
    25561 cttcggtata gccggacaga cggatgattt ccatacggtc gagcagcggt gtcgggatat
    25621 tcaggctgtt tgaagtcgca ataaacatca catcgctcaa atcgtaatcc acttcggcat
    25681 aatgatcggc gaacttgttg ttttgctcgg gatcaagcac ttcgagcaac gcgctggcgg
    25741 gatcgccccg gaagtcgcta cccaatttgt cgatttcgtc gagcaggaac aatgggtttt
    25801 ttacgccggc ttttgccata ttctgcaaaa tcttaccggg catggagccg atataggtgc
    25861 ggcggtgtcc ccggatttcg ctttcgtcgc gcacgccgcc caaagccatg cggacatatt
    25921 tccgccccgt tgctttggcg atggattcgc ccaaagaggt tttgcccacg cctggagggc
    25981 cgaccaggca cagaatcggg cctttgagtt tgtccatacg tttttggacg gcgaggtatt
    26041 ccaaaatccg ttctttgact ttttccaggc cgtagtggtc ggtatccagc accaatccgg
    26101 ctttggcgat gtctttgctg acgcgggatt ttttcttcca cggcagttcg agcagggtgt
    26161 cgatgtagtt gcgtacgacg gtggattcgg cagacatcgg cggcatcatt ttgagttttt
    26221 tcagttcgga caggcatttt tcttccgctt ctttgctcat ccccgccttt ttgatgcctg
    26281 cttccaaggc atccagttcg ccgttttcgt cttcttcgcc caattctttg tgtatcgctt
    26341 taatctgttc gttcagataa tattcgcgct gggatttttc catttggcgt ttgacgcgtc
    26401 cgcgtatgcg tttttcggcc tgcataatgt cgagttcgga ttccagctgt gccagcagga
    26461 attccatccg tttgccgatt tcgggaattt ccaaaatctg ttggcgttgc gccagtttca
    26521 actgcaaatg cgctgcgacc gtatcggtta gccggctgtt ttcggcaatg ccgttaatgc
    26581 tgccgataat ttcggcgggg atttttttat tgagtttggc gtattgttca aactgcgcca
    26641 acagggtgcg gcgcacggct tcgaggtcgg tattgccgcc cgtgtcttct tccacgaccg
    26701 tctctatatg ggaaacgaac agaccgcccg tgtcttcaat ggtcagaaca cgtccgcgat
    26761 acagcccttc gaccaatact tttaccgtgc cgtcgggcag tttcaacact tgcaggactt
    26821 gtgcgaccgt accggtctga tacaggtcgg cggcaaccgg ttcttctacc gccgcatcgg
    26881 tttgcgccaa caggaaaacc ggctcctcgc gggtaatggc gttttccagt gcggcgatgg
    26941 atttcggcct gccgacaaac agcggcagaa ccatatgcgg gtaaacgacg acatcccgca
    27001 aaggaagggt tgccaaggcg gcatattcct caaaatgctt ttctttttgt gtcataggta
    27061 ctctcttgtg tctgacagat tgccgatttt cgcgtacatt ggggttgaag gtattatttc
    27121 aagcatatgt ggtttattta tggagtttga tgcgatgccg tctgaaacat tccggcttca
    27181 gacggcatgg gcttggaaag acaaggcggg aacaaaaaac tgttctgtgt tgccgctcct
    27241 tgctgtacca tccgtatggt ttgcggttct gccgccctat tttcaaaaca tgacgcaggt
    27301 attccatgtc ttcttttatt tttcccgata acgggttcga atggcgcaac gaaagtctgc
    27361 aaaagccgcc caaaggttgg cattatgtcc gcgaaagtgg tgcaaacggc attttgaagg
    27421 ctgcctatca aaatattgca actgtctggc agggcgattt ccacaatgcc aaacaggtgc
    27481 tttccgcaat gaagaagaag gtccgcaaac ctgccgccgt ccgttccgat gcggatattg
    27541 ccgccctttt ccacgcccac cgtatgaagc aggcgcagca gagccgtatt ctgaatatgc
    27601 ttgccgttga aatccgcccc ggttttgtgt tggacaacaa acgcgcgccc gatatacgcg
    27661 ccgccctgct cgacgtgtac ggagaggcgg acggcaaacc gtttttcctg ccgctcaatc
    27721 tgctgctggg gtttatgggt gcgcacgagt ggcataagaa aggggttgcc gttccgcagc
    27781 tgggcggcag catacacgtt cctttcggcg tattctcgcc gttgcgcggc gaatacctcg
    27841 acctgctcgc ccatgcgccg tcaacgggtt ttcagacggc attcgatatc gggacgggct
    27901 cgggcgtgct tgccgccatt ttggcgaaac agggcattcc ttccgtcatc ggcacggata
    27961 ccaatccgaa agccgtcgcc tgcgcccgtg ccaatattgc ccgtttgggc tttgaaaaac
    28021 aggttgagat acgggaaacc gatctgtttc ccgaagggtt tgccgatctg attgtctgca
    28081 atccgccctg gcttcccgcc aagccgactt ccgccgtcga atccgcgtta tacgaccccg
    28141 aatctgcgat gctggctgcg tttttgcggg atgcgccgaa acatctgaat cccgacggag
    28201 aaatccgcct gattatttcc gaccttgcag agcatttggg gctgcgccct gccgattttt
    28261 tggaaaaggc atttatccgg gcgggtttgc gtgttgccga tatgatgaaa accaagccga
    28321 agcacaaaaa agccgcgaat ccgagcgatc cgcttgcttt tgcgcgaacc cgggaaacca
    28381 ctttcctata ccgtttgaaa aaggcataag gggcggcggc gcggcattcg ggcggattat
    28441 tcttgtgaaa atacccgctc gagcatactg cccaatgccg tctgacgcgt tttgacagtg
    28501 gcggcagctt gccggaaggt ttcttctccg ccgaagaata cgaacttttc cctcgcgcgg
    28561 gtaatggctg tgtaaagcag ccctctgctc aagcccgaaa gcgcgtcgtc cccttcgtcc
    28621 gaaggtgcgg cggaaggcgg cagcagccag acttcccgat attccgaacc ttggcttttg
    28681 tggacggtca tggcgaatgc gggttcgaaa tcgggcaggc agcttaccgc taccttttta
    28741 aatccgtccg catcggcaaa ataggcggca aggccgccct gccgtccgac atcttccata
    28801 atcaatccga tgtcgccgtt gaacagttca agcgcgtagt cgttctgtct gatcataatc
    28861 ggttctccgg cgaaataggc ctgatgttcc ggtatgttca ttttgcggcg tacatggctg
    28921 caataggctt cgttgaagtc ttccgcatcc tgccgccacg ccgcgaggac gacgatatcc
    28981 gagatgcctg cgtatgcggc ttcgatattg ccgtctttta ccgcctgcca ataggctttg
    29041 tgcgcccggt acaacctttc gactcgagcg ttcggactgc attccgaatg ttccagttcg
    29101 tccggaaacc ggtcaaacaa tgcccacgcc ccctcatcgc ccgatacggc ggcacgggca
    29161 aggcagccga tgccgctgtt gtcgccgaag cggtggctga acgacagatg ggcggtgttt
    29221 tgcgccaaca agggcggatt tgcactgacg ctgaaaccgt gttccggaag gaagccggcc
    29281 agcctttggt gcgtttctcc gtccaaaacg gttttttgcg acaggacgga cagcacgtcc
    29341 ccgattccga cggacgggag ctggttttca tcgcccagca gaatcacgcg cgcgccggtt
    29401 ttgaccgctt ttaaaagttg caacatcaat accgtatcca gcatagaggc ttcatcgata
    29461 accaatacgt caaacggcag cgggtaaaca gggttgaacg ccgcctgcat tttgggcggg
    29521 cgcagcttca gcagtcggtg gacggtttgc ccttccagtt tgagcaaatg gcggcggacg
    29581 gcctccggcg cgtcaaaacc gttgattgca cggtgcagcg cgcgcgccat atgtgccgcc
    29641 gctttgcccg tcggtgcggc aagcgcgatg ttgggaagat tttcgtcttc accgcaaatc
    29701 agcgccagca gtttggcaac cgttgtcgtt ttgcccgttc ccggcccgcc ggtaatcacc
    29761 ataaaagact gcaacagtgc caaggcggcg gcatcgcgct gcccttcgct gcccgtgcct
    29821 tgaaaccatt ttgcgaggtt ttgcctcgcg cctgccgcgt cgggggcgga cgtgtcggct
    29881 gccgccaagc gttttatctc ggcagccaaa tcgtattcca actgccacat cctgcccaaa
    29941 aacagccttc tgccttccaa aatcaaaggc gcggcggatg ttccgaccac gggtgcgagt
    30001 gccgacagcg cgtcagcctc gccaccgctc aaacggataa acgaatgacc gttttgcaat
    30061 gcctgaaaca ggcgttcggt gcagtttgca agcacttcgt cgctggaacc cgcatagcgt
    30121 gccaaaaacc agattgccgc ccttgccgcc gcttgggcaa attcatctgt ctgccgttcc
    30181 atcgtattcc ttgtcgaaat gccgtctgaa ggcgtggggc ttcagacggc gcggtgttgt
    30241 ttcggctgtt taggcgtttg cgccctgttt ggggtgcagg ctgccgtctt tcatgaccat
    30301 cacgcgcccg aagcggccgg cgagttcgtc gtcgtgcgtt acgaccacca gccccgttcc
    30361 caattctgtt ttcagttcca gcatcatatc caaaacattc cttgcgtttg ctcggtcgag
    30421 attgccggtc ggttcgtcgg caagcaggca tttgggttgc gtaaccaagg cgcgtgcaat
    30481 ggcggcacgc tgccgctcac cgcctgaaag ttcgcccgcg cggtgcgtcg aacggtgttt
    30541 cagtccgacc ttttccagca tcgccattgc cgtttctgct gcctcttcac ggcttttttt
    30601 gccgatcaga agcggcatca tcacgttttc cagtgccgaa aattcaggca gaagatggtg
    30661 gaactggtac acgaaaccga gatggcggtt acgtaaatcg cccaaacgcc gctggtttaa
    30721 ggtacgcaaa tcctcgccca tcagcagcac cctgccttca gacggcatat ccagcccgcc
    30781 tagaatatgc agcagcgtcg attttccgct gcccgaagaa ccgatgatgc cggtgctttc
    30841 ccccgcgcgg atttccaaat ccaagccgtg cagcaccgga acatccaaac cgccgtcgcg
    30901 gtagcgtttg cccacgcttt cgcatttcaa aatcaactca ctcataacgc aaagcctccg
    30961 ccggttgggt ttttgacgcg cgccggctcg ggtagagcgt ggcaacgaaa gacagtccca
    31021 aagaaatgca ggcaatcagg gcaacgtcgc ccatatcaac atcgctgggc aggtagtcga
    31081 taaaataaac ctgcgaattg ataaggtgga caccgagcag gttttcaaaa aatgccacga
    31141 ccctgccgac gttccaaccc aaaagcacgc cgcagaccac acccgccagc gtgccgaaaa
    31201 agcctgaaaa cgcgccctgc accataaaaa tcttcatcac gccgccaggg gaaagaccca
    31261 aagtccgcaa aatcgcaatg tccgcctgct tttccgtaac cgccatcacc aaggaagaga
    31321 caaggttgaa cgccgccacg gcgataatca gcgtcaggat gatgaacatc atccgttttt
    31381 ccaactcgac cgcctcaaaa tagctgcggt tgttgtacgt ccaatcgcgc acccaaaccg
    31441 cgtccctttg cgcctccgga atcagtgttg ccgtcaaggc gggggcgttt tgcgggtcgg
    31501 cgagtttcag ccgcagcccc gcaacttcct tatccaagcg gtacagtacg cgcgcgtctt
    31561 ggatatgcgt catcgccaat gaattgtcaa cctcgtaaac acccgtctta accagaccga
    31621 ccacggtaaa ctgtttcaac ctcggtacga ctccggcagg ggtaacgttg ccctccggcg
    31681 tgatgacggt aactttattg ccgacttccg cccccaaagc ctccgccaag ccgacaccga
    31741 ggataatgtc aaactcgccc ggaatcaggt cttcaaattt gcctgccggc attttgtcgc
    31801 catattccac cactttgcgt tcttcagacg gcaaaatgcc gcgcatctgc acgcccctga
    31861 tttcgcccgc gtttgccagc agagcctgat tggaaacata gggcgcggca gccaaaatac
    31921 ctttgcggtt ttccgtaaac cgaagcaggt tgcgccaatc cgtatccgta ttgtcgatat
    31981 agccgatttc ggcgtgcggc gcgacattca ggagctgccc gcgtatttct ttctgaaagc
    32041 cgttcataac cgacaagacg acaatcagcg cggttacgcc caaggcgatt ccggcaatcg
    32101 aaaccatcgt gataaacgac ataaagccgt tgcgcttttt cgccctgaga tacctcaagc
    32161 ctatccaagc ctctagagaa aacataacgc taccttaaaa atgtctgcaa acgtgccgcc
    32221 ccggacggcg gtttgggggc ggcggcaaaa gtttattgta ccgtaaaacc ccggcagcgt
    32281 ccgaacgccc gagtgcgggg gacggggcgg cagagcgggc ggaaaacttg cacaacgcgt
    32341 caaactgccc tatcctttcc ttcagaaaaa ccgtttcttg aggaaaacaa tgaatatccg
    32401 aactgctttt gctttgtgcg ccatcgcctt atccgccgct gcggctgcct acgccaaaga
    32461 aatcaaaatc gatgccaaca acacgcctta ttccgaagcc gacgcgcaaa agctggcggc
    32521 aacggcagtc ggtatgggtg ttaaggaacc tatcagcctg aacggcggca gcggcagcat
    32581 taccgtgtcc ggcagcagcg cgacacagtg cgtgttcaaa gtcggcaacg gaggcgcgtt
    32641 gcagattcaa gggctaaact gcaagtaaac cgcccggaaa aatgccgtct gaaggcttca
    32701 gacggcattt tgcattggcg gcgttacgcc ccgccttctt taatcaggcg gcgttcgtac
    32761 accgcctgcg ccagcgttcc cgcatcgaca tattccaatt cgccgcccaa ggggatgccc
    32821 tgcgacagcc tgctgacttt gtaaggcagg tttttaaaaa actcggacag gacatacgcc
    32881 gtcgcattgc cttctgcggt aaaagcggtt gcaataatga tttcttcgac ttccccgccg
    32941 cccagccgtt gcgccagcct gtccaatgcg atggcggata cgtccattcc caatgccgta
    33001 ttgatttgcc ccatcaggac gaaatacagc ccgtcgtggc agtttgccgc ttccatattc
    33061 gacacgtcgg caggcatatg caccaccatc agccgccgcc cgtcgcgtgt ttcatcggca
    33121 caaatatcgc acaatccgcc ttcgcaaaac gtgttgcaca tcgcgcaatg gtaaacctgc
    33181 ttcaatgccg tctgcaaggc atccaccagt ttttcagcct ctttgcgctt gtgttggagc
    33241 aaatgatacg ctatccgctg tgccgatttc ggcccaacgt tgggtaaaac cttcagcgcg
    33301 tcgatcaatc cttggaaggc atcttgtttt ttgtggctca tcatattccg ccgtatggga
    33361 aaacggccgg aatattccga ccgttatttt gtcaacaaaa gtgtcaatta ctgaccgtcg
    33421 ccgttgtcga ccgattgcgc tcctttggtc tgtttgattt tgccgttgaa ataacgtatc
    33481 aacaagtcga aagtattggc agactgctgt tgcgccaaag cctgttttgc aagcggaagc
    33541 tgtgcggcga tatcatccgg cggggttaca gcctgtactt cgacaatcac ggatgccggc
    33601 agaccgatca gcctgacgta ggcgggtttg ccgtttgccg gttttgcttt cagcagttcc
    33661 gcataagcct cgggcggcat ggactgcctt gcctgctgtg cgcccaaaac ggacacttcc
    33721 gaccatttca cgtcaacagc cttgccgccg ttcagttggg taagcacgtc ttttgccttg
    33781 ttttcggcaa gtttggcggc ttcggtacgg atataagcct gacgtaccgc gtctttggct
    33841 tcggcaaacg gcagggtttt ctcttcgcgg acttctttgg cgcggacgac ccacgcggtt
    33901 tcgctgttga tggtcagcac ttcggaattg tgttttttct tcaatacgtc gtcgctgaaa
    33961 acggcattga tcagattttc gggcataccg gacatttgcg cgtcctgcct actcagccaa
    34021 gtttcttggg tttcgacttt caaaccgctg tttttggcgg cttcggcaag cgaggaagga
    34081 tggttgaacg cgtcatcgcc cagcttttct tttgccttat tgaagtcggc aaccgccttt
    34141 ttcattttca attcgttttc tacggcggct ttttcctgct cgaaagaagg tttggcttca
    34201 tttgccggca aacgcgccac gcgctcttca aacgcatttt tcacttctgt ttcgctgacg
    34261 gtctgtttgt ctgcaaagtc tttcagattc aaggcgacat attccaattt gaccgctttg
    34321 ggaagcaggt agtctttttt gtttgcgtta taaaacttct gcaaatcggc ttcagacact
    34381 ttgacttggg cgatgaattc gtcggggttg aaagtgtgcg aacggatggt gcggttgacc
    34441 tgcgtcagcc tgatcagctg ttccgcctgc gcgtcgccga ccaatacgcc gttttggacg
    34501 aggtttacca aattctgcaa ggcaaactga tcgcggattt cttcgacaaa ctggtcttca
    34561 gacatatggc gttgcgacag gtagcggttt aaaagcgcgt ggtcgaattt gccgtttgcg
    34621 tcgtggaaat tgggatcatc cacgataatc tgcttgattt gttcggaaga aaccgaaatg
    34681 cccatcagct tcgcgccctg tttcaggtag gcgcgttgca gcagggattg gaacaccgcg
    34741 tcgcgcgaag ggccgccgcc gtccgcctgt tcgttctgta tggcgttgtt gatggagtgg
    34801 tcgctgattt tttcgtcgcc cacttggacg atgtagtcgg cacccggatg ggataccgtg
    34861 ctgaccccga agccgacgaa ggttaatgca atcaggccca aaaggacttg ggcgggcgtt
    34921 ctgtattttt cgatggaatg gaacatattt taaatcggga tatagaatgg gaacgggaaa
    34981 ttcaagtcgg gtattgtaac ggtttttatc cctgtctgca cggggcttgc cggttgaaga
    35041 tgccgtcgta ggtttcttcg ctgaagccgg cgtaaacctt gccgccgcac tccaatacgg
    35101 gacgcttgat caggctcggc atttcggaca tcagtttgac ggcctccgct gtcgagaaca
    35161 gggctttttg ctgtgtttcg gcatcgagtt tgcgccagct tgtcccgcgt ttgttgagca
    35221 gggttgccaa aggcacttgt tccagccacg agcagatttc cgcttcggaa ggcacctgtt
    35281 ttttaaaatc ccgaaattca aactccaagc cgtatccggc aagccggttt ttggcttttt
    35341 tgaccgtatc gcaatttggg atgccgtgaa ggactatcat ttggaaacct tttgtctgaa
    35401 ataataaaac ggatatttta ctataagtgt ctgaaaattt gcccgtctgt ttcagacggc
    35461 agggcggtta tgttacaatc cgaaaattcg aaaaatttaa tctcttgttc aataaaggct
    35521 ttaccaatca tgatttctac caacggcatc accatgcagt tcggcgcgaa gccgctgttt
    35581 gaaaacgtat ccgttaaatt cggcgaaggc aaccgctacg gtttgatcgg cgcgaacggc
    35641 tcaggcaaat ccaccttcat gaaaatcctc ggcggcgatt tggaacagac ggccggcgaa
    35701 gtggcgattg aaaacggcgt gcgtttgggt aaattgcgcc aagaccagtt tgcctacgaa
    35761 gacatgcgcg tgctggacgt ggtgatgatg gggcataccg aaatgtgggc ggcgatgacc
    35821 gaacgtgatg cgatttacgc caatcccgaa gccaccgaag atgactacat gaaagccgcc
    35881 gaactggaag ccaagttcgc cgaatacgac ggctacaccg ccgaagcgcg tgccgccgaa
    35941 ctgttgagcg gcgtgggcat ttccgaagat ttgcacaatg cgaaaatggc ggaagtcgcc
    36001 ccgggcttca aactgcgcgt attgctggcg caggcgctgt tctccaagcc ggatgtattg
    36061 ctcttggacg aaccgaccaa taacttggac attaatacca tccgctggtt ggaaggcgtg
    36121 ttgaaccaat acgactccac gatgattatc atcagccacg accgccactt tttgaacgaa
    36181 gtctgcacgc atatggcgga tttggactac aacaccatca ccatctatcc gggcaactac
    36241 gacgactaca tgctcgcctc cgcccaatcg cgcgaacgcg ccctgaaaga caatgccaaa
    36301 gccaaagaga aactgcaaga gctgcaagag ttcgtcgccc gcttctctgc caacaaatcc
    36361 aaagcccgtc aggcaaccag ccgtctgaaa caggccgaca aaatcaaatc ggagatggtc
    36421 gaagtcaaac cttccacccg tcaaaacccg tatatccgtt ttgaagccga tgaaaaagcc
    36481 aagctgcacc gtcaggctgt ggaagttgaa aaactggcga aacgctttga aacccagttg
    36541 tttaaaaacc tgaacttcat ccttgaagcg ggacaacgcc tcgccatcat cggcccgaac
    36601 ggcgcgggca aatccaccct gctgaaactc ttggccggcg cgtacaaccc cgaatattca
    36661 gacggcctgt taccggacga aggcaccatc aaatgggcgg aaaaagccag tgtcggctac
    36721 tatccgcaag accatgaaaa cgacttcgac gtcgatatgg atttgagcga atggatgcgc
    36781 caatgggggc aggacggcga cgacgaacaa gtcatccgcg gcactttggg gcgtttgctc
    36841 ttcggcagta acgatgtcgt gaaaaaagtg aaggttctct ccggcggcga aaaaggccgt
    36901 atgctttacg gcaaactgtt gctgttgaaa cccaatgtct tagtcatgga tgaaccgacc
    36961 aaccacatgg acatggaaag catcgaatcc ttgaacatgg cgctggaaaa atacaacggc
    37021 acgctgattt tcgtctccca cgaccgtcag ttcgtttctt ccctggcaac ccaaatcatc
    37081 gaactggacg gcaaaggcgg atatgaacac tacttgggcg attacgaaag ttacttggag
    37141 aaaaaaggcg tagcataacc gccggttgga acaatgccgt ctgaagccgc ttcagacggc
    37201 attgttgata actttaaaat aggaagcata tgcagactta cctcgtcggc ggtgccgtcc
    37261 gcgattatct tttgggcttg cccgtcaaag accgcgattg ggtggtcgtc ggcgcagacg
    37321 cacaaaccat gctggcgcaa ggcttccagc ctgtcggcaa agattttccc gtgtttctcc
    37381 atcccgaaac gcacgaagaa tacgccctcg cccgcaccga gcgcaaaacc gccaaaggtt
    37441 acgtcggttt cagtttccac gccgacaaag acgttacgct ggagcaggat ttgatgcgcc
    37501 gcgacctgac catcaacgcg atggcgcaag atgcggacgg caagattatc gaccctttcg
    37561 gcggacaacg ggatttggcg gcaggcattt tgcgccacgt ttcccccgcc tttgccgaag
    37621 accccgtccg catcctgcgt actgcccgct ttgccgcgcg ctacggattt gaaatcgccg
    37681 aagaaaccat gaagctgatg cggcagatgg tggcaaacgg cgaagcggac gcattggttg
    37741 ccgaacgcgt ctggcaggag tttgcgaaag gtttgatgga aaaaaatccg cgcaaaatga
    37801 ttgaagtgtt gcgcgaatgc ggcgcgctca aagtcttgct gcccgaagtc aatgccctct
    37861 tcggcgtgcc gcaacgcgcc gactaccatc ccgaaatcga cagcggcatc cataccctga
    37921 tgacgctgca acgcgccgcc aatatgggtt tgagcctgcc cgaacgctat gccgccctgc
    37981 tgcacgactt gggcaaagcc aaaacaccgt ccgacatcct gccgcgccac cacggacacg
    38041 acctcgccgg tgtcgaaccc gtgcgcgaag tcaatcagcg gctgcgtgcg ccgaaacatt
    38101 gcgccgagct tgccgaattg gtttgccgtt ggcacattat tttccaccaa gtcggacagc
    38161 ttaaaagcca aaccattctg aacgtattga aaaaaaccga tgccttccgc cgtcccgaac
    38221 gctttcagac ggcattgaac gtctgcattg ccgacacgca aggccgtctg aaccgcgaac
    38281 acacgcccta cccgcaacgc gcacactggc tcgccctgct cgaagccgcc aatcaggcgg
    38341 attcgggcaa aatcgccgcc gaatgccgcg cacagggaaa agcgcacctt atcgccgaac
    38401 aaatcgaccg ggcgcggctg gcacaaatcg ccccattgca aaaagcgttt cgagcggcgt
    38461 aagacaaaac agaaaaacat taaaacgtcc aatgcagcca
//
LOCUS       DAXHRI010000004        32667 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXHRI010000004 DAXHRI010000000
VERSION     DAXHRI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32667
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.004"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(68..814)
                     /locus_tag="ACFM75_000138"
     CDS             complement(68..814)
                     /locus_tag="ACFM75_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6254749.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(798..977)
                     /locus_tag="ACFM75_000139"
     CDS             complement(798..977)
                     /locus_tag="ACFM75_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254750.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRWFLIRTVYGFSF"
     gene            complement(993..1664)
                     /locus_tag="ACFM75_000140"
     CDS             complement(993..1664)
                     /locus_tag="ACFM75_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC6254751.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM75_000141"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="ACFM75_000141"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC6254752.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM75_000142"
     CDS             complement(2481..2963)
                     /gene="ispF"
                     /locus_tag="ACFM75_000142"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC6254753.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM75_000143"
     CDS             complement(2995..3684)
                     /gene="ispD"
                     /locus_tag="ACFM75_000143"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC6254754.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM75_000144"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="ACFM75_000144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC6254755.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="ACFM75_000145"
     CDS             complement(4571..5881)
                     /locus_tag="ACFM75_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6254756.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM75_000146"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="ACFM75_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC6254757.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="ACFM75_000147"
     CDS             complement(6056..6355)
                     /locus_tag="ACFM75_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254758.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="ACFM75_000148"
     CDS             6706..7902
                     /locus_tag="ACFM75_000148"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6254759.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8538..10379)
                     /locus_tag="ACFM75_000149"
     CDS             complement(8538..10379)
                     /locus_tag="ACFM75_000149"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC6254760.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10470..10940
                     /locus_tag="ACFM75_000150"
     CDS             10470..10940
                     /locus_tag="ACFM75_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC6254761.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(11011..11823)
                     /gene="hpnC"
                     /locus_tag="ACFM75_000151"
     CDS             complement(11011..11823)
                     /gene="hpnC"
                     /locus_tag="ACFM75_000151"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC6254762.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(11910..12392)
                     /locus_tag="ACFM75_000152"
     CDS             complement(11910..12392)
                     /locus_tag="ACFM75_000152"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6254763.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12865..13086
                     /locus_tag="ACFM75_000153"
     CDS             12865..13086
                     /locus_tag="ACFM75_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254764.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAAAT
                     EAPAAEAAATEAPAAEAPAAEAPAAEAAK"
     gene            13196..14563
                     /locus_tag="ACFM75_000154"
     CDS             13196..14563
                     /locus_tag="ACFM75_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC6254765.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14743..16782)
                     /gene="autB"
                     /locus_tag="ACFM75_000155"
     CDS             complement(14743..16782)
                     /gene="autB"
                     /locus_tag="ACFM75_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phase-variable autotransporter AutB"
                     /protein_id="HFC6254766.1"
                     /translation="MYYETRSKQASKQKFKKSFIVSLFFSILYTSPLLAVDYVYDKTK
                     LTNDEITRLKKLRDKTSEYWKKETYLITEDNPKVPPFPALYPRTYQFENINNSKKISF
                     YDQEYTEGYLVGFARGLGVAKRNGDTEEQIRKYFKECFNSNTKIRDYSTCQAEKFGSH
                     PLIVKSHIFSLGPKIKNSHINSEILSVGNYTEWANQVIHHIENYVSFAAHLYSGLDPF
                     HYIEVTDNSHVIGQTISLDEFRLENSLWEPRWDSNVGKLKTTNADIRFNTKSESLLVK
                     EDYAGGARFRFAYDPKEAKNTALIFEKNVTGTSDIIFENPIDDLKSLDGHQIIKVNGT
                     ADKHAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFNLRLNN
                     KNNDIFDRTLPRKGLWLRVIDGHSNQWVQGKTAPVESYRKGVQLGGEVFTWQNESNQL
                     SIGLMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQ
                     RFRHSINTEDATERFTSKGITASIEAGYNALLAEHFTEKGNRVRFYLQPQAQLTYLGV
                     NGKFSDSENAQVNLLGFRQLQSRVGVQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGLE
                     MDGERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(17147..17593)
                     /gene="smpB"
                     /locus_tag="ACFM75_000156"
     CDS             complement(17147..17593)
                     /gene="smpB"
                     /locus_tag="ACFM75_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC6254767.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17648..18658)
                     /gene="waaF"
                     /locus_tag="ACFM75_000157"
     CDS             complement(17648..18658)
                     /gene="waaF"
                     /locus_tag="ACFM75_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC6254768.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18789..18864)
                     /locus_tag="ACFM75_000158"
     tRNA            complement(18789..18864)
                     /locus_tag="ACFM75_000158"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18829..18831),aa:Asn,seq:gtt)
     gene            complement(19055..19807)
                     /locus_tag="ACFM75_000159"
     CDS             complement(19055..19807)
                     /locus_tag="ACFM75_000159"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC6254769.1"
                     /translation="MITLLSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEASYPDSLNELVCAAIATPLGEMLAVFGSKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20118..20642
                     /locus_tag="ACFM75_000160"
     CDS             20118..20642
                     /locus_tag="ACFM75_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFC6254770.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     AASLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     ALCGTSSSASTAAA"
     gene            20996..21106
                     /locus_tag="ACFM75_000161"
                     /pseudo
     CDS             20996..21106
                     /locus_tag="ACFM75_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(21479..22624)
                     /gene="dapE"
                     /locus_tag="ACFM75_000162"
     CDS             complement(21479..22624)
                     /gene="dapE"
                     /locus_tag="ACFM75_000162"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC6254771.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYEGILACLLAGNAV"
     gene            complement(22737..23357)
                     /locus_tag="ACFM75_000163"
     CDS             complement(22737..23357)
                     /locus_tag="ACFM75_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFC6254772.1"
                     /translation="MLTSKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQVWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     KLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(23382..23891)
                     /locus_tag="ACFM75_000164"
     CDS             complement(23382..23891)
                     /locus_tag="ACFM75_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6254773.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQITRYFNVAAPLHHEDEEEDFFPLLLQYAPQAREGVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDAEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(23986..24537)
                     /gene="azu"
                     /locus_tag="ACFM75_000165"
     CDS             complement(23986..24537)
                     /gene="azu"
                     /locus_tag="ACFM75_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC6254774.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAVGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(24818..25240)
                     /locus_tag="ACFM75_000166"
     CDS             complement(24818..25240)
                     /locus_tag="ACFM75_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC6254775.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            25371..28121
                     /gene="secA"
                     /locus_tag="ACFM75_000167"
     CDS             25371..28121
                     /gene="secA"
                     /locus_tag="ACFM75_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC6254776.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHTLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            28136..28288
                     /locus_tag="ACFM75_000168"
     CDS             28136..28288
                     /locus_tag="ACFM75_000168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254777.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            28266..30038
                     /gene="dnaG"
                     /locus_tag="ACFM75_000169"
     CDS             28266..30038
                     /gene="dnaG"
                     /locus_tag="ACFM75_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC6254778.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKKQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            30225..32153
                     /gene="rpoD"
                     /locus_tag="ACFM75_000170"
     CDS             30225..32153
                     /gene="rpoD"
                     /locus_tag="ACFM75_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC6254779.1"
                     /translation="MSRNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            32300..>32667
                     /locus_tag="ACFM75_000171"
     CDS             32300..>32667
                     /locus_tag="ACFM75_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6254780.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSIFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKL"
BASE COUNT         8300 a         9257 c         7648 g         7462 t
ORIGIN      
        1 ataaatttac cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccagcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcatagt cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtatcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc gcccaaaagc
     1621 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatctctga accggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tattccgttt tcgagtacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggttt gtcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaaggg
     1981 aacgcgcgct ttcgacgact tccacgggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcgtc cgcaatgcag acgaattttt ctgatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatcctt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacagcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcattgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagccccg cgcggaaaag
     3181 ctgcggcgtt tgtgcctgcc aaaggctcgt ccgctcgaca gtggcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaatgga aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcagc gcgcggcatc
     3361 gtgtaccaga atattgtcat ccgccgatgc caagccgatt tccaacagtt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctgtatc gtcaagccgt ggacgcgtgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacagag caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcacgca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacggacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaataaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aggcacgctt tggcaacgcc
     7801 ggcgtgatta ccactgccgg cagcaaagcc gttgccgtgg tcattccgac caacgaggaa
     7861 ttgatgattg cacacgatac cgcccgcttg agcggtctgt aagattcaat ccccacgcga
     7921 actgcctccg aaaacggagg cagttttttt atccggcttt ccatgcttaa acagcaccgc
     7981 ctcttttcag acgttgacgg ttgcagccgc ttacctgaac cttatagtgg attaacaaaa
     8041 accagtacag cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctcaagca
     8101 ccaagtaaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     8161 tttgttaatc cactatatcc gactttccat acttaaacag cactgcctct tttcagacat
     8221 tgacggttgc agccgcccac ctgaacctta atgattgact attttttaat catgttatta
     8281 ttttccataa aatacatgac attaagatgt ttttccacaa aagatacaca caccggcaaa
     8341 caccggctgt gtttatcttt tcttatgcct attttttaat catcgtattt ttatctttta
     8401 atttcaatac gcaaactaac ttatacacac ggttttcaca tctttggact gcttccgtgt
     8461 gtatagcgga tattgccgtt ttcctttctg acaaaaatgc cgtctgagaa cttcagacgg
     8521 catttgaaac atcggaatca gcggttttgt tcataccact cgataaactt gtctgctttg
     8581 acaaaaccca gcagcggctc gctgcggctg ccgtcggagc ggacgacaaa cacgcccggc
     8641 ggcccgaaca gaccgtattc tttcaacaac gcctgatgtt cgggcgtgtt ggcggttacg
     8701 tcgatttgga aaaagcgttc catatcgact gcctgatgca cttccggctg attgagcgtg
     8761 taagccgcca tttctttgca ggaaatgcac cagtcggcat aaaaatccaa aacgacgggt
     8821 ttgtcgggat gttctttcaa cgccgtatcc atcgctgcct tcagcgcggc agtatcggca
     8881 aacattttgc cgtgttccga agatttgcct gcttcggccg gcggattgag ggtcaggaaa
     8941 tggtgcagcg cggtcgtttt gccgtttgcg ccctgccagc cgaaccacgc gccgcctatc
     9001 agcaatatgc tgcccaatgc gaatgccaca gctttcggac ggcgtttctg cctgcgtccg
     9061 ttgaccagca gcatacaggc aggaaccagc atcagcagcg tgtacagcgc gacgacgaga
     9121 taatagggca agtgcggcgt ggcgaggtaa acggcgacgg caagcaggat aaagccgaaa
     9181 gcgtatttga cggcattcat ccaatcgcct gccttaggca ggatatgccc gccgaacgtg
     9241 ccgatggcaa tcagcggaac gccggtgccc aacgccaaag tgtaaagtgc caaaccgcct
     9301 aaaaccgcat cgcccgtctg accgatgtag cccaaagcaa atgccagcgg cggggcgacg
     9361 cacggcccga caatcagcgc ggacaatatg cccataataa agacggaaac gattttaccg
     9421 cctgaaagcc tgctgctttg attctgaaaa tacgactgca cggcgttggg aagctggatg
     9481 ttgaacagcc cgaacataga cagtgccaag acgaccatca aagccgatgc cgccaacacg
     9541 acccaagcct gctgcaacca tacggtcagc agtgcgcccg tcagtccggc aacaatgccg
     9601 accagcgtat aagtcagagc caaaccctga acataaacga cggacagcac aaacgcccgc
     9661 gccttgcccg cctttttgtc gccgaccaca atactggaaa caatcggcaa caggggatac
     9721 atacaggcgg taaaactcag acccaaaccg gcgagaaaaa acgccaaaag attggcgttg
     9781 agcgtatccc aagacagctt gaaacggctg tcgccgccct catccccctt cgggggcggc
     9841 agcgccccgc tgccgttttg agaggaaggc tgcaaaaagc ggtctttggc ggatgccggt
     9901 tcgtcggttt gcggatggta agtgccgttg ccggaaatat caaactcggt atccacgggc
     9961 ggatagcaca cgccggcttc ggcacagccc tgataggtca aaaccaattt atacggttcg
    10021 ccgacagcct ttgcataagg aaaggcaacc tgcgcttcgt gatggtaaac cgtctgcctg
    10081 ccgaaaaact cgtcttcctt ctcttcgccc ttgctgaaag aaggctgtcc caacaaatcc
    10141 gccggatcgg tcttgccgac gattttcgcc tgatacatat agtatccgtc ggcaatcttg
    10201 aaacggacgt tcacaccgtc gtcggcaacg gcaagctccg gcacgaatgc cttttccggc
    10261 ggcagcagat cgttcgcatc cagcgcgaaa gctcgtccgc acaacatcaa aaatacggcg
    10321 aacaggcaaa tcagtttttt cataatcgaa tccgtttcag acaaataatt tgtctgcatt
    10381 ataaatggta aggttgacgg tgggatttaa tttatgtaaa acccgccatt atccgaacct
    10441 atttccataa ccatcttatc gaacccgcca tgtacgatgt caatacccac gatgtccgcc
    10501 gctttttcgc ccgcgtgtgg cagcagcggc tcaatccgct gcaactgagc gcactggaac
    10561 agaaagccct ccgcattgtc gaagcccatc ccgaatacca ccgctatctc gaacgcatcg
    10621 aagaccatct ggacaccgac tggctgcccg aaaacggcga aagcaacccc ttcctgcata
    10681 tgtcgctgca tctgtccgtc caagaacagg cgggcataga ccagccgcac ggcatacgcg
    10741 caatccacga caccctgtgc gccaaacgcg gctggctgga agccgaacac gaaatgatgg
    10801 aggcactggc ggaaacactg tggacggcgc aacgctacgg caccggtttg gatgtcaatt
    10861 tctacatgac ccgactgcgc aaactcatcg gcttgggtgc agaagatcaa gccaggttga
    10921 acccacatga aatcgcctga ccataccaac cgcctgcaaa atgccgtctg aagcggaaca
    10981 acccctttca gacggcatgc atttccccca tcatttccac aacgcgcgtt tcagcataat
    11041 caaccaatcc ttcttatcca aaacggggcg ttgtgcaaac acatcgtatc ggcacgcgtc
    11101 cagtttctgc aaaatcaact gcgcccctaa cacaatcata cggagttcca aaccgatacg
    11161 ccctttcaat tcgcgcgcta aaggcgaacc cgccttcagc atacgaaatg cgcgccggca
    11221 ctcatacgcc atcagccgct gaaacgccgc atccgcccta ccctccgcaa tctgtccttc
    11281 ggaaacaccg aatttcaaca aatcgtcctg cggaatataa accctgccct tttgccaatc
    11341 cacagccaca tcctgccaaa aattcaccag ttgcaaagcc gtacagatgc cgtcgctttg
    11401 cgccacgcac accgcatccg ttttcccgta caaagccagc ataatgcgtc cgacaggatt
    11461 ggcagaacgt cggcaataat cggccagctc gccgaaattt ccataccttg ttttaaccac
    11521 atcctgagaa aatgcagaaa gcaaatcata aaacggctgc aaatccaaac cgaacggcac
    11581 aaccgcctcg gcatccaatc gtgcaatcaa aggatgcgcc gaccggccgc ccgatgccaa
    11641 cacgtccaac tcgcgccgca aaccctccaa ccccgccaac ctggcttcag acggcatact
    11701 gccctcgtcc gccatatcgt ccgccgtccg tgcaaacgcg tacaccgcgt gaaccggctt
    11761 cctcaacctg cgcggcaaaa tcagcgaacc gacgggaaaa ttttcataat gcccaaccga
    11821 cataccttct ccatccatca aacaaaatgc cgtctgaaat ggaacaaacc cttttcagac
    11881 ggcatcagat acctccaagc tgccggcaat cagtggtggt gatgaccgtg cgggccgtgg
    11941 acatgaccgt gtgcgatttc ctcatcggat gcatcgcgca cactttcaac cgtagcctta
    12001 aagcggattt tcatgcctgc caaaggatgg ttgccgtcca ccaccgcctt accgtcggca
    12061 acatcggtta cacgatagac gacaacatcg ccggtttcag gatcgtcggc ttcaaacatc
    12121 atgccgactt cgacttcaac agggaacacg cccgcatctt caatacggac caactccgga
    12181 tcctgctcgc cgaacgcatc gtcgggcgaa agcgccacat cgaccgtatc gccggcatcc
    12241 ttaccgtgca acgcctcttc caccaaaggg aaaatgccgt cgtaaccgcc gtgcagatac
    12301 gcaatcggtt cttcggtttt gtccaaaagc tgattgttgg catcatacat ctcataatgc
    12361 agcgaaacca cggaattctt cacgatagcc atatttgtcc tttcaggaac agcagattga
    12421 ttacaggcgc attctaacac aaccgccgcg ccggccgatt accgttaacc tgttcataaa
    12481 ctgtacagca catatttcaa tgtaaatttt tgttatttta ttgcgatgta acttttttac
    12541 aacattatta aaaccattcc gacctgtctg ccgactttcc caatccgcct taataaatca
    12601 tacaagatac tgaaattata ttaatctcta taatatttat ccctatcgaa tttttaacag
    12661 caaaaccgtt ttacaggatt tatcaatccg cccgccagaa aacttttcat tcaaaccttt
    12721 ttcccatctg tacgacattg caatccctta ttccatagtg cataattacg caaattcagc
    12781 gatgaatttc caacccggtt tgtagtatgg tcgataaaga cctatttgtt tcaataattt
    12841 aaattggttc taaaggttac taaaatgaaa aaatccctgt ttgccgctgc tttgttgtct
    12901 ttggtcctgg cagcctgcgg cggtgaaaaa gccgctgaag ctcccgctac cgaagcacct
    12961 gccgccgaag ctcccgctgc tgaagctgcc gctaccgaag cacctgccgc tgaagctgcc
    13021 gctaccgaag cacctgccgc tgaagctcct gctgccgaag ctcctgctgc cgaagctgca
    13081 aaataagcat tttccgcttg caaaaaagca ggatacgttc agtatcctgc ttttttgatt
    13141 tttcagacgg catcagattc ccttcctcaa tcttctccct acccttccga caaacatgcc
    13201 tgaccttcat accgaatttt cccgactcct gccggcagat gaaattgccg aactttctcc
    13261 gacgctttta aaagaccagc gcaaccgctt tacgtctgca ccagacatca ttttgcagcc
    13321 gcgcagcgtt gaaagcgtgc aaaccattat gcgtttttgt tacgaacacc gcattccggt
    13381 tacgccgcaa ggcggcaata ccggtttgtg cggcgcggca gtatcggaaa acggcgtatt
    13441 gctgaacctt tccaaactca accgcatccg cagcatcaat ttgtcagaca actgcataac
    13501 cgtcgaagca ggttccgtac tccaaaccgt ccaacaggca gccgaagcct caaacaggct
    13561 gttcccactc agtctcgcca gcgaaggctc ctgtcaaatc ggcggcaaca tcgcctgcaa
    13621 tgccggaggt ttgaacgtat tgcgttacgg cacgatgcgc gacctggtta tcggtttgga
    13681 agtcgtcctc cccaacggcg agctggtttc ccatctccat cccctgcata aaaacaccac
    13741 cggctacgac ctgcgccatc tgtttatcgg cagcgaaggc acactgggca tcatcactgc
    13801 cgccacgctc aagctttttg cccgtccctc ggacaaggca acggcatggg tcggcatacc
    13861 cgacatcgaa tccgccgtcc gcctgctgac cgaaacccaa gctcactttg ccgaacgcct
    13921 atgcagtttt gagctgatcg gccgttttgc cgccgagttg tcttccgaat tcagcaaatt
    13981 ccccctgccg acacattcag aatggcatat tttacttgag ttgaccgact cattacccga
    14041 cagcaatctt gatgatcggc ttgtcgaatt tctttataaa aaaggattta ccgacagcgt
    14101 attggcacaa agcgaacaag aacgtatcca tatgtgggca ttgcgcgaaa acatctccgc
    14161 atcgcaacgc aaactgggca ccagcatcaa acacgatatt gccattccta tcggacgcgt
    14221 tgccgacttt gtccgccggt gcgccaaaga tttggaacag aattttaaag gcatacaaat
    14281 cgtctgcttc ggacatcttg gcgacggcag cctgcactac aatactttcc tgcccgaaat
    14341 cctcagcaat gaagtctatc gttacgaaaa cgacatcaac gacgcagttt atcgcaacgt
    14401 tcttgcctgc aacggcacga ttgccgccga acacggcata ggtatcatca aaaaacagtg
    14461 gctggacaaa gtacgcacgc ctgccgaaat cgccctgatg aaaagcatca aacaacacct
    14521 tgatccatat aacattatga atccgggcaa actgcttccg taaccggcat ttctgatttg
    14581 catacacaac aaagaaaggg acaatagatc cgattgtcgg tttagcgcga gctcgtgagt
    14641 gcggtaaaaa attggtggaa atgacacgaa aaatgaccgc atttttaaaa taaaaaatcg
    14701 gcagtaaatt tctctgccga ttttattttg ttacaactta acttaaaacg tccactgtaa
    14761 attcaacgca ccttgtttag cctgatgatg tttgcctgtt tggcggttga atgttgcttg
    14821 taaagttaag tgagatttga ttttcactgc tacacctaat tggctctcaa ttgccgtctt
    14881 attgtttatc actcgacgct ctccgtccat ttccagaccg aaaggttttt gttggtagat
    14941 tgaattgaca gcaacaaatg gttggaaagt gacgccatta gtgaaagcaa attgagcttt
    15001 agcttgaacg cccactcggc tttgtaattg gcggaagcca agcaaattca cttgggcatt
    15061 ttcgctatcg ctgaattttc cgtttacccc caaataagtc aattgtgcct gtggttgcag
    15121 gtaaaaacga acacggttgc ctttttcagt gaagtgttct gccaataaag cattgtaacc
    15181 cgcttcaatt gaggcagtga taccttttga agtaaagcgt tctgtagcat cttcagtgtt
    15241 aatgctatgg cggaaacgtt gatattgtac ccagctatcc gcatacgcac ctgtctgttt
    15301 gtcctgaagt tgatgccaag tggcgtaaac gcctgcacca aagcctttca catttcccgt
    15361 tgtaagattg tctgtatctg ggttgcggaa agtactgcgt tgttctgctt gtccgcccat
    15421 taagccaata gaaagttgat tactttcgtt ttgccatgtg aatacttcgc cgccgagttg
    15481 cacacctttt cgatagcttt ctacaggtgc tgttttgcct tgcacccatt ggttggaatg
    15541 tccgtcaatc acacgcaacc acaagccttt gcgtggtaaa gtgcggtcga aaatatcgtt
    15601 gtttttgtta ttcaaacgta ggttgaataa agtattggcg gcttgagcct gttgtgcata
    15661 aatcgcaata tcatcgcgtt cttgcacttt ggtaaaaaag ccctctgggc gttgttgtaa
    15721 agaaagcgta taaattccct tttggtgttt gccagaaaga cggaatgcgt gtttatctgc
    15781 tgtgccattt actttgataa tttgatgccc atcgaggctt tttaaatcgt ctattggatt
    15841 ttcgaagatg atgtcggaag tgccagtaac atttttctca aaaattaatg cagtattttt
    15901 cgcttcttta ggatcgtaag caaaacgaaa acgagctccg ccagcataat cttcttttac
    15961 gagtaaactt tcacttttag tattaaaacg gatgtctgca ttcgttgttt ttaatttccc
    16021 aacattagaa tcccaacggg gctcccagag agaattttct aagcggaatt catccaaact
    16081 aatcgtttgc ccgataacgt gcgagttgtc tgtaacctca atatagtgga atggatctaa
    16141 accagaatat agatgtgctg caaaagaaac ataattttca atatgatgaa ttacttgatt
    16201 agcccattct gtataattcc cgacagataa aatttcgctg ttgatatgac tattttttat
    16261 ttttggacct aaggagaata tatgactttt tactataaga ggatgggatc caaatttttc
    16321 agcttggcaa gtactataat cacgtatctt agtgttagaa ttaaaacatt ccttaaaata
    16381 tttccgtatt tgttcttctg tgtccccatt tctttttgca acccctaaac ctcgggcgaa
    16441 gccaactagg taaccttcgg tatattcttg atcataaaaa gaaatctttt ttgagttatt
    16501 gatgttttcg aattggtatg ttctagggta tagtgcggga aagggtggaa cttttggatt
    16561 atcctcggtt ataagataag tttctttttt ccaatattca ctcgttttat cgcggagttt
    16621 ttttaagcgg gtaatttcat cattagtgag cttggttttg tcgtaaacgt aatcaacagc
    16681 caaaagcgga gaggtataaa gaatagaaaa aaatagactt acaataaatg attttttaaa
    16741 cttctgcttg cttgcttgct tgcttcgagt ttcataataa attttccttt gtcaagtaaa
    16801 aataaatagg gcgtggattt tagcataaaa ctgaacaaaa aatgtcattt agttcaaatt
    16861 ttctctatgt atttcttatt tattaaaagt aaacgtttgc tttttgctat tttgtcaagc
    16921 cagtttgaaa atgtgtataa ttgccctcgt tatttacaaa aatttcagga aaaatgaccg
    16981 cactttaccc ttggctaatg ccaatttatc atcaaattgc tcaaaccttt gacgaaggtt
    17041 tggggcatca tgctgtgctg attaaagcgg atgctggttt aggtgtagaa cgtttacaca
    17101 tcaggcggca gccttgccca taccctctga agcactgttt ccacaatcag cgcgtatgct
    17161 taatcaaccg ctgtttctcg cgtttccaat ccgcctcttt catactctga cgtttgtcgt
    17221 gctgtttctt accttttgcc aaaccgattt ccatcttgat ttttccgcgc gaaaaatgca
    17281 aatccagcgg cacgatggtg tagccggcac gttcggtttt gccgattaat ttgttgattt
    17341 ccgactggtt caacaagagc ttgcgcggac gtacggcatc tggtttaatg tgtgtcgagg
    17401 ctgtgggcaa agccgtaata tggcagccga ccagataaaa cgcgtctttt ttccaataga
    17461 tataactctc tttaagctgt acgcgcgcgg cgcggattgc tttgacttcc cagccttcca
    17521 agaccaaacc ggcttcaatc cggtcttcaa tgaaaaaatc gtgaaatgct tttttattgt
    17581 tcgcaatagc cataaacatc ctatcaatat ccgccgtcag acggcataaa cccgaaaaca
    17641 gaacccatca taccgcctct tcaaccgcct gcacaatctt ctcgggatac agcctgttga
    17701 ggcagtcggt atgccccagc ggacattctc gtttgaaaca cggggaacat tccaagtgca
    17761 ggctgacgat tttcgccctg tcgctcaaag gcggcgtatg cgtcgggctg gaagaaccgt
    17821 aaaccgccac taccttcctg cccaaagctg ccgccaagtg catcaatccg ctgtcgttac
    17881 acacaaccgt atctgccaac gacaacaaat ccattgcctg cgacaaatcg gttttgccgc
    17941 acaaattgac acacataccg tctgaaaggc ggttgatttc ctcggcaatt tcatcatctt
    18001 tttgcgaacc gaacaaccaa acctgccaac ccgccgccag ataatgtttg cccaactcgg
    18061 caaaatgcct tgtcggccaa cgctttgccg gcccgaattc cgcgcccggg caaaatgcca
    18121 gaacaggctt tccaatatcc aagccaaagg tttcgacaga aatttcccgc cgccgttcat
    18181 caatggaaaa ctcggggaat cccgaatgcc cgtcaaaatc ctcctggctc ggatgcgcga
    18241 gagccgtata tctgtccacc atcaaaggca gacgttcctt atccagcctg cgtatatcgt
    18301 tcaacagaaa ataacggctt tcaccgacat aacccgtcct tttaccgata cctgtcgcca
    18361 gcgcgatgac tgccgatttc aaagaaccgg gcaacacaat aacctgatcg tatccgcgcc
    18421 gccccaaatc cctgccgacc cgccagcggc gtttcaactc caacgcaccg tgtccgaacg
    18481 gattttcaag aatctcattc acttcaggca tacgctcaaa caccgccatc gaccacttcg
    18541 gtgcgaacac atcaatcgtg caaccgggat gaagttcctt caaacggcgg aacaagggct
    18601 gggtcatcac gcagtcgcct atccaactgg gagaaataat caggattttg atggacataa
    18661 caagaaaccg aaatcagaca ggcagaattt taccgcgaaa ccgttggaaa acctatcttg
    18721 ccgcattccg aacgccgtac gtgcaaatat gaaaaagccc gaacattcaa gttcgggctt
    18781 caaaattctg gctccccgac ctgggctcga accagggacc tgcggattaa cagtccgtcg
    18841 ctctaccgac tgagctatcg gggaatgggg cgtattatag cgtccggaaa aaatgtgtca
    18901 atccttaatt ttggaaaaat gggcgacaaa acgacaagca tatgaatcag aaagacatta
    18961 agaccgatgc cttaaaagga ttgccgttgt atgaatttcc acagccgtca tcacaccata
    19021 tttaagcccg atgagccgtt ctgccctccc ccgcttaaaa caatgccgtc tgaacttcgc
    19081 cgcgttccaa agccagtaaa aactgtttgc ggttcaaccc gcccgcgtag ccggtcagtt
    19141 tgccgtcgct gccgatgacg cggtggcagg gaatcaagat agacaccttg ttctgcccgt
    19201 tggcggcggc aacggcgcgg acggcgttgg ggttgcccaa acgctgcgcc tgctctttgt
    19261 agctgcgcgt ttcgccgtaa ggaatcgcca agagcgcgtc ccatgcctgc ttttgaaact
    19321 cggtgccaat ctgctccaaa ggcgtggcaa aggttttcag acgacccttg aagtataagt
    19381 ccaattcctg ccgcaaaagt tgcgtccgct catcctcccg aaacacaaac cgtccgcgca
    19441 aggctttttg gacggcggca atttcctgtt ccaaatgctt ctgtccgaca aactccagca
    19501 gacacaagcc tttactgccg aacaccgcca gcatctcgcc caaaggcgtg gcaatggcgg
    19561 cacacaccag ctcgttcaaa ctgtcgggat aactcgcttc cagcagacgg atggcgcggc
    19621 ggatacggac atattcttca ggcgcacagc cgatattgtc ccaaaaatcc cgctcgaact
    19681 gcccggcttc gcattccgtc aaattgggat gcggcataac gccgcactca caaacccggg
    19741 tttcgagcca atagcggatt tcatcccatt ttgacggcag attgtttaag gaaagcaggg
    19801 taatcattcg tttgctccgt atctctatca tggatttggc ggcaaaatcc ccaatttttg
    19861 ccattcccgc acgccggagc aggaacgggc tatgacgtaa atcttgaggg ttaggttgcg
    19921 gcaatgccta aatatgcgat atttctaaag cctcagggaa aggaattaaa gacttcagac
    19981 gacctgcaaa gggtcgtctg aaaccacgat ttttgcattt acgcattccg gcacatcatc
    20041 caaccgtttc ggcacattcc tgccgccgtt gacagcctat aatgaatcca cttattcatc
    20101 aagcaaagga atcatctatg caaacccgca tcctctccgc cgtactgctg gctttttcaa
    20161 ccgctgcctt tgccgggggc gcattcacgc tgcaattcga caacccgtcc gaagacggcg
    20221 gcttcacgca aaaccagctt ttgagcgcgc cttacggctt tggctgttcg ggcggcaatg
    20281 cttcgcccgc gctgtcgtgg aaaaatccgc cggccgggac aaaaagtttc gtcctgaccg
    20341 tttacgataa agacgcgccg accggactgg gctggatgca ctgggtggtc gccgacattc
    20401 ccgccgatgt ccgccgccgc aacgcggcct cgctgcaatt aagccgctgc gccaacatcg
    20461 ccgacgacca gtccgcagcc atatcggcgg taatcagttt gcggatttgc cgcatcaggt
    20521 tgacgccttc gtacacggca aaaccgatgc cgtcatgctg caaccacgcc aacacgccgc
    20581 aaagcgcggc ctccgccgca ttgtgcggca cttcttcatc cgccagtacc gcagccgcat
    20641 aatcaaacgc ctcgcccatc cgcccgggtg cggcagcttc accgcatcgc acactgcctg
    20701 cgccgtcccg tattgtgcgg caaacctttc tatggcttcc tgttcgaaag caatccattg
    20761 cgcctgatag aggccgtctg aatcgggaat attaataacg tcaaacgtct gtccgcctgc
    20821 caacgcgacc gccttacccg ccgcaatcct caattcgcgc cccgcccacc gcagcttctt
    20881 acttccgcgc cgcacgataa gcacagccgg ttcatatacc gccacgctgc ggtacaaggc
    20941 ggtatgatgt tgcacgatgc cgcctaaagc acccaatcgt tcgcgcgtat gaaagtatag
    21001 tggattaaat ttaaatcagg acaaggcgac gaagccgcag acagtacaaa tcgtacggca
    21061 aggcaaggca acgccgtact ggtttaaatt taatccacta tatctcaaac ccacgttagg
    21121 tctaagcaaa tggtcggaca tccttatccg acagcccatc ttcttttcag acggcattgc
    21181 aaatttaagt ttgacgtgcg ttcaaaataa ggcagttaat gcgaagcgaa attccgtcgg
    21241 cgtacctgca acttggcccc tcccctatag gggagggtcg gagggagggt aaaacggggc
    21301 agatacagac aatatttccg ttaccgcccc gatgccctct ccctagccct ctcccacggg
    21361 agagggaatg gattgccgtt caaataaaca accctacata aaaatcaata tgttaactca
    21421 aatttacgtt aggtctaagc aaatggtcgg acatccttat ccggcaagca agctgctttc
    21481 agacggcatt tccagccaac aagcacgcca atatcccctc atacaccgca gacagcttcg
    21541 gaatatcgtc cagccgcacg ttttcgttga tttggtggat ggtggcattg gacgggccta
    21601 attcgatgag ttcttttgca atggctttga tgaagcgtcc gtccgaagtg ccgccggtgg
    21661 tggacaattc ggcctcaatg ccgcaggttt cggcaatggc tgcgcgtgcc acgtcggtca
    21721 gtttgcccgc ttgggtcaga aagggctgcc ccgaacacga ccactgcaaa tcgtattgca
    21781 cgccgtgttt gtccaaaatg gcgtggacgc gttgtttcag ccctgcttcg gtggactcgg
    21841 tggagaagcg gaaattgaat ttgacgctca gctcgcccgg aatgacgttg gtcgcgcctg
    21901 tgccgccgtt gatattggaa atttgaaagc tggttggcgg gaaatattcg ttgccttcat
    21961 cccagacttc ctgcgtcagc tctaacaagg ccggggcaaa agtatggacg ggattgattg
    22021 ccaaatgcgg ataggcaata tggccttgct tgcctttgac ggtcaggttg cccgacagcg
    22081 agccgcgccg gccgttttta atcatatcgc ccaatttgtc cacggctgtc ggttcgccga
    22141 cgatgcagta gtcgataagc tcgtcgcgcg ctttcaatac atcgacgact ttggtcgtgc
    22201 cgtccaacgc gtcgccctct tcgtcggaag taatcagaag cgcaatgctg ccttggtggt
    22261 cgggatgttc ggcaacgaag cgttcacagg cggtaacgaa gcaggcgatg ctggttttca
    22321 tgtctgccgc gccgcgcccg tataatcttc cgtcgcgctc ggccggttcg aacgggggcg
    22381 aatcccattt ttcgacagga cctgtcggta caacgtcggt atgccctgca aaacagacga
    22441 cgggagcttt cgtgccgcgt cgcaaccaga tgtttttggt gtcgccgaaa tggagttctt
    22501 cagccgcaaa accgattttg tgcaggcgtt cggcaaggag tttttggcaa tccctgtcgt
    22561 caggggtaac ggatggtcgg gaaatcagct ctttggcaag ctctagggat tgagtttcgg
    22621 tcatatttgt tcacttttga aattagaccg tctgaaacgt tctgaatgtg attttcagac
    22681 ggcatttagg ttaggttggc atacggggtg ggtattttac ccatcagtct tctgaatcat
    22741 ttgccgtggc aggcttcgta aagcggcagc aaatcttcca ccgtttccgc tatccatttc
    22801 gcgacatcct gcttgcccaa atcgtcgcgc tcgatgtgtt tgccgatgca gaaaaagtct
    22861 tcgtcgtttt gcaactttcg gtcggattcg ctttgttgtg cgacggtgcg gtagtcgtca
    22921 tattcgcttt ccgcgccgtg ccacatatcg aatgcggcgt atttgtcggt atcgaaatca
    22981 tccaaccagc ggttgtattc gggcagcgca atcggcgaca cgtcggcttt gtagcagtgc
    23041 caatccaagc tgacgctcag gcggcggcgg ttcaataaaa tcgataaaat cgcggcggaa
    23101 tttttatatt gttcgtattt gaagtaggca aagaaatggg cgcgcacctg ccagccgtta
    23161 caccagcgtt cgatatgcgg cggcgcgaac ggcgcaccca attcggcggc aacctgctgg
    23221 atcagctgct gccatacctg ccagttttct ttatagtctg ccttgatttg cggaatgctt
    23281 tcaggctggt attttttaag ctgggaaaat tggaaaaacg ggatattgaa caaatcgcaa
    23341 cttttcgagg taagcatagt gtattccttg agacgattac cttatttgcg ccgacgcgcc
    23401 gccatgattt caccgatttc ggtcagtttt tctttaggga taaaggtgtt gcccatatca
    23461 aacagcggct cttcaatcgc caaatgaaca tcatatcccg ccacaaaacg tttgaacgct
    23521 tccgcatcgg gaacataagc attgtctgct tcgagcttgg caaattcggc ggaaacagcc
    23581 gcccagttgt cgtgcagccc gatatgttgg cgtaaaagct cgtccacacc ttcacgtgct
    23641 tgcggcgcgt attgcagcag cagcgggaaa aagtcttctt cttcgtcctc atgatgcagc
    23701 ggcgcggcaa cgttgaaata ccgggtgatt tggcggatgg tttgcaaaac aatttgattg
    23761 cagccgtttt cggcgatgta gtccgacagc atggcgactt gtccgcaaaa acggcgcact
    23821 ttgccgtggc aggcatacag catttcaatc ggttcggcaa aggtaacgct tttggtttca
    23881 aacggattca tgttttcgtt ctcaacgggc acttttcaag cagtcatttt ataataaaac
    23941 agcctgcaca aagcaggctg tccgtctttt gagactttaa gcggattaat cgaccaaagt
    24001 cactttgccg ttcatcaaag caccgtgacc tgggaaggta caagcgaatt tatattcgcc
    24061 gtcggccaat ttagcaggat ccagagtcag ggaagcttct tcgccgccgc cgatcagttt
    24121 ggtatgggca acaacgcgtg catcatcagg tttgacatag tcggtatcgg cagcacctac
    24181 gccgtcttta aatacgccgt ccatgtcttc agctttggca atcacgagat tgtgacccat
    24241 gctggctttg ggttgcgtac cggtatgttt cagagtgatg gtaaactctt tacatgcttt
    24301 gctgacttgg atgtctttgg tgttgaactg catattgtcg ttggattcga cagttgccgc
    24361 acagttgccg acagcagggg cttcggcagc atctgcagga gcagcttcgg cggcaggtgc
    24421 ttcggaagcg ggtgcttcag cagcaggagt tgcctcggca gcaggcgcgg caggttcttg
    24481 agagcaggca gccaaaccga taacggcagc agaaatcaga gccagatacg ctttcataac
    24541 aaatctccaa tcgataaaat aatattcggt tttacagaaa tcaaagtgca accgccatta
    24601 acaaaacctt gaaaaagatt ccgccgcgtt gcacaaacag atgtttcgga gcggcatttt
    24661 gctacaaatt tcatttgaaa tcaaagcctg tctgcaagtt tacaatcgtt tactttaaaa
    24721 ggggcaattt taccccgaac ctatttcttt agtattagac ccattatcct ttacttctta
    24781 atattaacgg atgtttacac aaattcccgt atacatttta tgcgccatgc cttctaacca
    24841 agtttgccaa tgcctccgcc aattcgggat gccgtttttc caactttgcc gccgccgaac
    24901 cgaaactctc cagcgcagcc ttactcaaat gcagggtatt ggttttcggc ggtttttccg
    24961 gtttcgggac cagcctgacc gaaacagagc gtatcgaagc atcaagccct gccaactgcg
    25021 gcaataccga cggtgcaatc attttcaagc gcgatgccgc catattgttt gccgccaaaa
    25081 ggacaagcct gccgtcttcg atacatgccg tctgaaaatg cgggtgcagg ttggcaggca
    25141 gcagtttttt cacggcggca tccaaccgcc gccactgtcc cgcctgtttc aaaagtccgg
    25201 aaagcagcgc gtcccgcctg cccaactgtt ccaaattcat aaaacataca cccaaaaaga
    25261 ttgaaatacc gcaaacgcgc ctttatttca gacggcatta gcactttgca caaacgcttg
    25321 tgttaaaatc gcgttttcgc ccactattat atcaggcgca ggaattattc atgctgacaa
    25381 acattgccaa gaaaatcttc ggcagccgca acgaccgctt gctgaaacaa taccgtaaat
    25441 ccgttgccag aatcaacgcg ctcgaagaac agatgcaagc cctaagcgat gctgatctgc
    25501 aagccaaaac tgccgaattc aaacaacgcc tcgccgacgg tcagactttg gacggcattt
    25561 tgcccgaagc cttcgccgtc tgccgcgaag cgtcccgccg caccctcggt atgcgccact
    25621 tcgacgtgca gcttatcggc ggtatggtgc tgcacgacgg caaaatcgcc gaaatgcgta
    25681 ccggcgaagg caaaaccctg gtcgccaccc tcgccgtcta tctcaacgcg ctggccggca
    25741 aaggcgtaca cgtcgttacc gtcaacgact acctcgcctc acgcgatgcg ggcatcatgg
    25801 aaccgctcta taatttcctc ggccttaccg tgggcgtgat tatttcagat atgcagccgt
    25861 tcgaccgtca aaacgcctat gccgccgata tcacctacgg caccaataat gaattcggct
    25921 tcgactacct gcgcgacaat atggttaccg accaatacga caaagtgcag cgcgaattga
    25981 attttgccgt tgtcgatgaa gtggattcca tcttgattga cgaagcgcgc actccgctga
    26041 ttatctccgg ccaggcagac gacaacatcc agttgtacca aatcatgaac accgtcccgc
    26101 cccacctcgt ccgccaagag acagaagaag gcgaaggcga ctactgggta gatgaaaagg
    26161 cgcaccaggt catcctgagc gaagccggcc acgaacacgc cgagcagatt ctgacccaaa
    26221 tgggattgct ggcagaaaac gactcgctct attctgccgc caacatcgcc ctgatgcacc
    26281 accttatggc ggcattgcgc gcgcatacgt tattccacaa agaccaacat tacgttatcc
    26341 aagacggcga aatcgtcatc gttgacgaat ttacaggccg tctgatgtcc ggccgccgct
    26401 ggtccgaagg cctgcatcaa gccgtggaag ccaaagaagg cgtggaaatc aaacgcgaaa
    26461 accaaaccct cgcctccatc accttccaaa actatttccg cctgtacacc aagctctccg
    26521 gcatgaccgg cacagccgat accgaagcct tcgagttcca aagcatctac aacctcgaaa
    26581 ccgtcatcat cccgaccaac cgccccatac aacgcaaaga cttcaacgac cagattttcc
    26641 gttccgccga agaaaaattc gaagccgtcg tcaaagacat cgaagaatgc cacaaacgtg
    26701 ggcagcccgt cctcgtcggc acaaccagca tcgaaaactc cgaactggtc tcccaccttc
    26761 tgcaaaaagc cggactgccg cacaacgtcc tcaacgccaa agaacacgaa cgcgaagcac
    26821 tgattgtcgc ccaagccggc aaagtcggcg cgattaccgt tgccaccaat atggcgggac
    26881 gcggtacgga catcgtttta ggcggcaacc tgaagcacca aaccgatgcc atccgtgccg
    26941 acgaagcctt gagcgacgaa gagaaacagg cacaaatctc cgcactcgaa gacggctggc
    27001 aggcagaaca cgacaaagtg atggaagcag gcggtttgca catcatcggt acggaacgcc
    27061 acgaaagccg ccgtatcgac aaccagttgc gcggacgttc cggccgtcag ggcgaccccg
    27121 gatccagccg tttctacctc tcgtttgaag acccgctgct gcgcctattc gcactcgacc
    27181 gcgccgccgc catcctcaac cgcctcgccc ccgaacgcgg cgttgccatc gagcacaacc
    27241 tgctgacgcg ccaaatcgaa ggggcgcaac gcaaagtcga aggcagaaac ttcgatatgc
    27301 gcaaacaggt tctggaatac gacgacgtcg ccaacgagca acgtaaagtc atttacagcc
    27361 aacgcaacga aattttaacc agcaaagaca tcagcgacct gatgcaggaa atccgttctg
    27421 atgtcgtcag cgacctcgtg gatacctata tgccgcccga cagcatggaa gaacaatggg
    27481 acatcccgac tttggagaac cgtctggctg ccgaattcag actgcacgaa gacatccaat
    27541 cctggctgaa ggcggacaat gcgattgacg gtcaagacat caaagaacgc ctgatcgaac
    27601 gcatcgaaaa cgaatatgcc gccaaaaccg aactggtcgg caagcaggca atggccgatt
    27661 tcgagcgcaa cgtgatgttg caggtcatcg acaaccaatg gcgcgaacac ctcgccgcta
    27721 tggactacct gcgacaaggc atacacctgc gcagctatgc ccaaaaaaat ccgaagcagg
    27781 aatataaacg tgaagccttt accatgttcc aagacctgtg gaacggcatc aaattccata
    27841 ttgcctccct gcttacctcg gttcaaatcg aacaaaaccc tgtcgcggtg gttgaagagc
    27901 aacccatcgg caacatccag tccatccatt ccgaatcgcc cgatatggaa gaacttttgg
    27961 gtcagtcgca aaccgatttg gttaccgaag cctttaatcc cgatgggaca gatttcagcc
    28021 ccgaagcctt ggaagcgcgg gggcaaatcg tccaccgcaa cgacccctgc ccctgcggca
    28081 gcggtttgaa atacaaacaa tgccacggca aactggctta agcgtttgaa cgcaaatgcc
    28141 gtctgaacat cccgctcccg tttcagacgg cattttgcct gaaccgccac atccgactgc
    28201 cattccgaaa aatcccgatt tcgtaccgtc cgtaccaaaa acagacatcc cgtccgcccc
    28261 acatcatgat tccatccgac ttcattgacg agcttttagc caaaaccgat attgtcgata
    28321 ttatcgacga gcaggttccg ctgaaaaaag gcggggcgaa ctatatggcg tgttgcccgt
    28381 tccacaagga aaaaacgccg tcgttttcgg tcagtccaac caagcagttt taccattgtt
    28441 tcagttgcgg ggcgcatggt tcggcgattg gttttgtgat ggagcatcag gggctgtcgt
    28501 ttccggaggc tgtgcagttt cttgccgacc gtgtgggcat ggttgtgcct aaagtgcgtg
    28561 ggcagaacga taatcccgaa gtccgtgccg aacgtaagaa aaaaaagcag acgctggaag
    28621 aaacgacggc ggcggcggct gatttttacg cgcaacagct aaaattcaat ccggcggcaa
    28681 aagcttattt ggacaagcgc ggcttgagtg cagaagttat cgcgcattat ggtttgggct
    28741 acgcgcccga cggctggcag cctttggcgc aagtgttcca accgtatccg aataccgcgt
    28801 tggtggatac ggggatggtg attgacaatg aggggcggta ttacgaccgc ttccgccatc
    28861 ggattatgtt ccctatccgc aatccgcgcg ggcaggtcat cggtttcggc ggcagggtgc
    28921 tggacgactc caaacccaaa tatctaaact ctcccgatac acctttgttt gacaaaggga
    28981 aaaaccttta cggcttgtat gaggggcgtg ccgctgtcaa ggaagcggag cggattctgg
    29041 tggtcgaagg ctatatggac gtggtcgcgc tggcacagtt cggcgtgggc tacggtgtgg
    29101 cggccttggg cacggcaaca acagctgaac acgtcaaaat cctaatgcgt caggcagaca
    29161 gtatttattt ctgtttcgac ggcgacagcg cggggcgaaa agcggcttgg cgcgcgctgg
    29221 aaaacgcgtt gccgcagttg aaagacgaca aatcgctgca ttttttgttc ctgcccgagg
    29281 aacacgaccc cgacagttac atccgtgcct acggcaaagc gcaatttgaa gacgcgcttc
    29341 tgaatcaaag caagcctttg tcggagtatt tctgggaaca cctttcagac ggcattcatc
    29401 tcaatacgca ggaaggcaag gcggaattgg tgaaaaccag ttcgccgctt ttggcgcaga
    29461 ttaccgcgcc ggcattggct tatttgttaa aacaacggct tagcgaactg gtcggcatcg
    29521 accccgacaa cctcgcgcaa ctgctaggac aggaagcgcc gaagcggcac gtcaaacaaa
    29581 aaaactacaa actgcctccg atttccgtca aacagcccgt catgctgacg ctggtacagc
    29641 ggcaaatccg cagcctcttg ataaatccgg attgggctgc atatatagac ctgcccgatt
    29701 atctggcgtt ggacggtgat ttcgcctgcc ttgccaatct tgccgaatcg attaaaaacc
    29761 atgccgccgt acccgaaacc gctcaggttt tagagtatat gcgcggctcg ccttacgaag
    29821 aaacgataac ccgaatcttc cattcaacgc accaatcgga agaaatgaac agcagcagtg
    29881 aagaagattg cgagaatttc caaatcggca tgaaaaaact gctcaatgag ttaaaataca
    29941 gccaaatcga aacattaaaa caaaaaagcc tgcaatccgg cttaaatgaa agcgagaaaa
    30001 aacttttgct gtcgttgctg accgcaaaac aaaattgacc ggcggattcc gccacccgta
    30061 aacccgttat gccgtctgaa aagcacttac cccggctgca acaacgacac ctgcagaaca
    30121 cccatcccaa aaagccttca gacggcatca gagtacccgc tctgccacgc cttcaggtgc
    30181 gttcaaacgc aaaccgtcgg caccttacca acagaaagca gacaatgtcc agaaaccaaa
    30241 atcacgaaga ataccaagac gatgcccgcc ccctgacgat tgaggaacaa cgcgctcgcc
    30301 tgcgccaact catcattatg ggcaaagaac gcggctacat cacctactcc gaaatcaacg
    30361 acgcactgcc cgacgatatg tccgatgccg atcaaatcga caacatcgtc agtatgattt
    30421 ccggtttggg tatccaagtt accgaacacg cccccgatgc ggaagacata ttgttaagcg
    30481 acaatgccgc cgttaccgac gatgatgccg tcgaagaagc cgaggccgcc ctttccagcg
    30541 cagattccga gttcggcagg acgaccgacc ccgtccgtat gtatatgcgc gaaatgggac
    30601 aggtcgacct gctgacccgc gaagacgaaa tcatcatcgc caaaaaaatc gaaaacgccc
    30661 tgaaaaatat ggttcaggcc atctccgcct gcccgggatc cattgctgaa atcttagaac
    30721 tcatcgaaaa aatccgcaaa gacgaaatcc gcgtcgacga agtcgtagaa gccattatcg
    30781 acccgaatga agtattgctc aacgaattgg gcttggggca cttggaaacc acagcacccg
    30841 agaaaccttc caacgacaat tcggacgaaa acgaagacga cgaagaatcg gaagaagatg
    30901 cggatgaaat ctcggcagcc aatctcgccg aattgaaaca aaaagtcatc ggccactttg
    30961 cccaaatcga aaaagactac aaaaaaatga tcggccgttt ggaaaaacac cacagccggc
    31021 acaaagacta tctcgcctac cgcgacgcga ttgccaacaa actgctggaa gtccgtttcg
    31081 ccacccggca aatcgacagc ctcagcagca gcctgcgcgg aaaagtagaa aacatccgca
    31141 aactcgaacg cgaaatccgc gacatctgcc tcgaccgcgt ccatatggag cgcgactact
    31201 tcatccaaaa cttcctgccc gaaatcacca atctggaatg gattgaagaa gaaatcgcca
    31261 aaggcagggt ttggagcgac gcgctcgacc gcttccgcca cgccatcctc gaaaaacaaa
    31321 ccgagttggc ggatatggaa aaagaaaccc gcatttccat cgaagagttg aaagaaatca
    31381 acaaaaatat ggtgtcgagc gaaaaagaaa ccgcagccgc caaacaggaa atgattcagg
    31441 caaacttgcg cctcgtgatt tccatcgcca aaaaatatac caaccggggc ttacaattcc
    31501 ttgatctgat tcaggaaggc aacatcggtt tgatgaaagc ggtcgataag ttcgaatacc
    31561 gcagaggcta taaattctcc acctacgcaa cctggtggat ccgccaggca atcacccgtt
    31621 cgattgccga tcaggcgcgt accatccgca ttccggtaca tatgattgaa accatcaaca
    31681 agatgaaccg catctcgcgc caacaccttc aagaaaccgg cgaagaaccc gattccgcca
    31741 aacttgccga actgatgcag atgcccgaag acaaaatccg caaaatcatg aaaatcgcca
    31801 aagagccgat ttcgatggaa acccccatcg gcgacgacga cgattcgcac ttgggcgact
    31861 tcatcgaaga tgccaacaat gttgcgccgg ccgatgcggc aatgtacacc agcctgcacg
    31921 aagtaaccaa agaaatcctc gaaagcctga caccgcgtga ggcaaaagtc ctgcgtatgc
    31981 gtttcggcat cgatatgaac accgaccaca cgctggaaga agtcggcaga cagtttgacg
    32041 taacgcgcga acgcatccga caaatcgagg caaaagcact ccgcaagctg cggcatccga
    32101 caagaagcga ccgtttgaga agtttcttgg acagcgaaga cagcaagctg taaaccaaaa
    32161 aaccgcaggt ttcaaatacc tgcggttttt tcttacacaa taaacaacgc ttccacatat
    32221 cccacactcc tatcccgaga cctttgcaaa atcccccaaa atcccctaaa ttcccaccaa
    32281 gacatttagg agattttcca tgagcacctt ctttcaacaa accgcgcaag ccatgattgc
    32341 taaacacatc gaccgtttcc ctctattgaa gttggatcaa gtgattgatt ggcagccgat
    32401 cgagcagtac ctgaaccgtc aaaaaacccg ttaccttaga gaccaccgcg gccgtcccgc
    32461 ctatcccctg ttgtccatat tcaaagccgt cctgctcgga caatggcaca gcctctccga
    32521 tcccgaactc gaacacagcc tcatcacccg catcgacttc aacctgtttt gccgctttga
    32581 cgaactgagc atccccgatt acagcacctt atgccgctac cgcaactggc tggcgcaaga
    32641 cgacaccctg tccgaattgc tcaaact
//
LOCUS       DAXHRI010000005        32636 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXHRI010000005 DAXHRI010000000
VERSION     DAXHRI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32636
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.005"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            230..2371
                     /gene="fetA"
                     /locus_tag="ACFM75_000172"
                     /gene_synonym="frpB"
     CDS             230..2371
                     /gene="fetA"
                     /locus_tag="ACFM75_000172"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC6254781.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGGDDHRIVLSHMKDQHRGIRTVREEFAVGDTKSRINIKR
                     QAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TQITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSQFKIEDKKDATDEEKKK
                     NRDNENIAKAYRLTNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSSS
                     NLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARNT
                     EIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHGYELGASY
                     RTGGLTAKVGVSHSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFQNPNLEIGW
                     RGRYVQKAVGSILVSGQKNREGKLENVVRQGFGVNDVFANWKPLGKDTLNVNLSVNNV
                     FNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            2531..3496
                     /locus_tag="ACFM75_000173"
     CDS             2531..3496
                     /locus_tag="ACFM75_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6254782.1"
                     /translation="MLRLTALALCTALALGACSPQNSDSAPQAKEQAVSAAQTESASV
                     TVKTARGDAKVPQNPERIAVYDLGMLDTLNKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            3660..4628
                     /locus_tag="ACFM75_000174"
     CDS             3660..4628
                     /locus_tag="ACFM75_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6254783.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGSVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            4618..5592
                     /locus_tag="ACFM75_000175"
     CDS             4618..5592
                     /locus_tag="ACFM75_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC6254784.1"
                     /translation="MPSENKIAFMQGSPRPLWAAFALLLICCGLFLTLNVKGDWDFVL
                     QLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTLGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSVAAVWRERYRLDVYLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            5637..6263
                     /locus_tag="ACFM75_000176"
     CDS             5637..6263
                     /locus_tag="ACFM75_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC6254785.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAQDGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGIQGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALKPFAPQ
                     MRDIMPKAVSGSASTDELKHFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            6346..7104
                     /locus_tag="ACFM75_000177"
     CDS             6346..7104
                     /locus_tag="ACFM75_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6254786.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLACTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPSENDKTIVEEALAEFHLQDFSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKVALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            7119..7262
                     /locus_tag="ACFM75_000178"
     CDS             7119..7262
                     /locus_tag="ACFM75_000178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254787.1"
                     /translation="MPSESLSDGTPYPDKIKARHRRHWRQHNMHILTDINPEPPAGKN
                     LNK"
     gene            7417..8392
                     /locus_tag="ACFM75_000179"
                     /pseudo
     CDS             7417..8392
                     /locus_tag="ACFM75_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
     gene            complement(8458..8796)
                     /locus_tag="ACFM75_000180"
     CDS             complement(8458..8796)
                     /locus_tag="ACFM75_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC6254788.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKIKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            8891..9103
                     /locus_tag="ACFM75_000181"
     CDS             8891..9103
                     /locus_tag="ACFM75_000181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254789.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRTETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            9261..13247
                     /gene="purL"
                     /locus_tag="ACFM75_000182"
     CDS             9261..13247
                     /gene="purL"
                     /locus_tag="ACFM75_000182"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC6254790.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSSVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLRDVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVAPYAT
                     EGNPFGKKPFNAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQ
                     TPVADCAVTMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIG
                     NIKLSANWMAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEK
                     KSVVSPLSLIISAFAPVKDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYN
                     NMSGDAPDLDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLD
                     IDLNLLLAQTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVA
                     DIINLFYQQQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRL
                     RDNPACADSEFALIGDNGRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQ
                     IEMAAAFTRAGFDAYDVHMSDLMAGRIHLADFKMLAACGGFSYGDVLGAGEGWAKSIL
                     FHPALRDQFAAFFADPDTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARL
                     SMVHVPKSASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQV
                     TQTYPLNPNGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLF
                     AGARKALG"
     gene            13342..14100
                     /gene="gloB"
                     /locus_tag="ACFM75_000183"
     CDS             13342..14100
                     /gene="gloB"
                     /locus_tag="ACFM75_000183"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC6254791.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLARERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            14414..18889
                     /gene="mspA"
                     /locus_tag="ACFM75_000184"
                     /gene_synonym="ausI"
     CDS             14414..18889
                     /gene="mspA"
                     /locus_tag="ACFM75_000184"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HFC6254792.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLIHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTQTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDSFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFIGNNRQQAAKQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNGPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDMTGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYDLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQI
                     HVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNP
                     LKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARI
                     DSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELD
                     TYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNL
                     AELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHR
                     PYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENG
                     ALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSN
                     GNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALS
                     VNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            19121..20575
                     /gene="mgtE"
                     /locus_tag="ACFM75_000185"
     CDS             19121..20575
                     /gene="mgtE"
                     /locus_tag="ACFM75_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC6254793.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(20661..21569)
                     /gene="hslO"
                     /locus_tag="ACFM75_000186"
     CDS             complement(20661..21569)
                     /gene="hslO"
                     /locus_tag="ACFM75_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC6254794.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            21814..22569
                     /locus_tag="ACFM75_000187"
     CDS             21814..22569
                     /locus_tag="ACFM75_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6254795.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            22588..22806
                     /locus_tag="ACFM75_000188"
     CDS             22588..22806
                     /locus_tag="ACFM75_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254796.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            22951..23295
                     /locus_tag="ACFM75_000189"
     CDS             22951..23295
                     /locus_tag="ACFM75_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC6254797.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            23295..23987
                     /locus_tag="ACFM75_000190"
     CDS             23295..23987
                     /locus_tag="ACFM75_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC6254798.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTVVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            24054..25274
                     /gene="argJ"
                     /locus_tag="ACFM75_000191"
     CDS             24054..25274
                     /gene="argJ"
                     /locus_tag="ACFM75_000191"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC6254799.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFNEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVVD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQSAATVYTCD
                     LSHGYVSINADYRS"
     gene            25334..26743
                     /locus_tag="ACFM75_000192"
     CDS             25334..26743
                     /locus_tag="ACFM75_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6254800.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            26933..28324
                     /gene="hrpA"
                     /locus_tag="ACFM75_000193"
     CDS             26933..28324
                     /gene="hrpA"
                     /locus_tag="ACFM75_000193"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC6254801.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYQKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKAIAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLIGKDEDDAEIELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            28425..29552
                     /locus_tag="ACFM75_000194"
     CDS             28425..29552
                     /locus_tag="ACFM75_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC6254802.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKIATEPPSALRENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            29729..31306
                     /gene="lpt3"
                     /locus_tag="ACFM75_000195"
     CDS             29729..31306
                     /gene="lpt3"
                     /locus_tag="ACFM75_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC6254803.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGG
                     AGASMLDKLWLPALWGMLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQSAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            31372..>32636
                     /locus_tag="ACFM75_000196"
     CDS             31372..>32636
                     /locus_tag="ACFM75_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6254804.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VPYGKVTPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYHERLPDFYTADAVSDGLRPANP"
BASE COUNT         8095 a         9866 c         8034 g         6641 t
ORIGIN      
        1 caggcttcgt cattcccgca tttgttaatc cactatattt ccgccgtttt ttacatttcc
       61 gacaaaacct gtcaacaaaa aacaacactt cgcaaataaa aacgataatc agctttacac
      121 aaatcccccc tctcgttaat ataaacaaaa ataattatta tttttcttat cctgccaaac
      181 cttaacggtt tggatttact tcccttcata cactcaaaag gacgaacaaa tgaacgcccc
      241 gtttttccgc ctcagcctgc tctcgcttac cctggcggca ggttttgccc atgcggcaga
      301 aaataatgcc aatgtcgcat tggataccgt taccgtaaaa ggcgaccgcc aaggcagcaa
      361 aatccgtacc aacatcgtta cgcttcaaca aaaagacgaa agcaccgcaa ccgatatgcg
      421 cgaactctta aaagaagagc cctccatcga tttcggcggc ggcaacggca cgtcccaatt
      481 cctgacgctg cgcggcatgg gtcagaactc tgtcgacatc aaggtggaca acgcctattc
      541 cgacagccaa atcctttacc accaaggcag atttattgtc gatcccgctt tggttaaagt
      601 cgtttccgta caaaaaggcg cgggttccgc ctctgccggt atcggcgcga ccaacggcgc
      661 gattatcgcc aaaaccgtcg atgcccaaga cctgctcaaa ggcttggata aaaactgggg
      721 cgtgcgcctc aacagcggct ttgccagcaa cgaaggcgta agctacggcg caagcgtatt
      781 cggaaaagag ggcaacttcg acggtttgtt ctcttacaac cgcaacgatg aaaaagatta
      841 cgaagccggc aaaggtttcc gcaatgtcaa cggcggcaaa accgtaccgt acagcgcgct
      901 ggacaaacgc agctacctcg ccaaaatcgg aacaaccttc ggcggcgacg accaccgcat
      961 cgtgttgagc catatgaaag accaacaccg gggcatccgc actgtgcgtg aagagtttgc
     1021 cgtcggcgac acaaaatcac ggataaatat taaacgccaa gcccctgctt accgcgaaac
     1081 cactcaatcc aacaccaact tggcgtacac cggcaaagat ttgggctttg tcgaaaaact
     1141 ggatgccaac gcctatgtgt tggaaaagaa acgctattcc gccgatgaca aagataacgg
     1201 ctacgcaggc aatgtaaaag gccctaacca tacccaaatc accactcggg gtatgaattt
     1261 caacttcgac agccgccttg ccgaacaaac cctgttgaaa tacggtatca actaccgcca
     1321 tcaggaaatc aaaccgcaag cgtttttgaa ttcacaattt aaaattgaag ataaaaaaga
     1381 tgcaactgat gaagagaaaa agaagaaccg tgacaatgaa aacattgcca aagcctaccg
     1441 tctgaccaac ccgaccaaaa ccgataccgg cgcgtatatc gaagccattc acgagattga
     1501 cggctttacc ctgaccggcg ggctgcgtta cgaccgcttc aaggtgaaaa cccacgacgg
     1561 caaaaccgtt tcaagcagca accttaaccc gagtttcggc gtgatttggc agccgcacga
     1621 acactggagc ttcagcgcaa gccacaacta cgccagccgc agcccgcgcc tgtatgacgc
     1681 gctgcaaacc cacggcaaac gcggcatcat ctcgattgcc gacggcacga aagccgaacg
     1741 cgcgcgcaat accgaaatcg gcttcaacta caacgacggc acgtttgccg caaacggcag
     1801 ctacttctgg cagaccatca aagacgcgct tgccaacccg caaaaccgcc acgactctgt
     1861 cgccgtccgt gaagccgtca atgccggtta catcaaaaac cacggttacg aattgggcgc
     1921 gtcctaccgc accggcggcc tgactgccaa agtcggcgta agccacagca aaccgcgctt
     1981 ttacgatacc catcctaaaa aactgttgag cgcgaacccc gagtttggcg cacaaaccgg
     2041 ccgcacttgg acggcttccc ttgcctaccg cttccaaaat ccgaatctgg aaatcggctg
     2101 gcgcggacgc tatgttcaaa aagccgtggg ttcgatattg gtgtcaggtc aaaaaaaccg
     2161 cgagggcaaa ttggaaaacg ttgtacgcca aggtttcggt gtgaacgatg tcttcgccaa
     2221 ctggaaaccg ctgggcaaag acacgctcaa tgttaatctt tcggttaaca acgtgttcaa
     2281 cacgttctac tatccgcaca gccaacgctg gaccaatacc ctgccgggcg tgggccgcga
     2341 tgtacgcttg ggcgtgaact acaagttcta aaacgcacat cccgaaaaaa tgccgtctga
     2401 aagcctttca gacggcatct gtcctgattt ttgttaatcc actatagatt atcatttatc
     2461 cttttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat ccccattttg
     2521 gagacaccct atgttgcgtt tgaccgcatt ggccctctgc accgccctcg ccttgggcgc
     2581 gtgttcgccg caaaattccg actctgcccc acaagccaaa gaacaggcgg tttccgccgc
     2641 acaaaccgaa agcgcgtccg ttaccgtcaa aaccgcgcgc ggcgatgcaa aagtgccgca
     2701 aaaccccgaa cgcatcgccg tttacgattt gggtatgctc gacaccttga acaaactggg
     2761 cgtgaaaacc ggtttgtccg tcgataaaaa ccgcctgccg tatttagagg aatatttcaa
     2821 aacgacaaaa cctgccggaa ctttgttcga gccggattac gaaacgctca atgcctacaa
     2881 gccgcagctc atcatcatcg gcagccgcgc cgccaaagca tttgacaaat tgaacgaaat
     2941 cgcgccgacc atcgaaatga ccgccgatac cgccaacctc aaagaaagtg ccaaagagcg
     3001 catcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc tgaaggcgga
     3061 aatcgatgct tcgtttgaag cggcgaaaac tgccgcacaa ggcaaaggca aaggtttggt
     3121 gattttggtc aacggcggca agatgtccgc cttcggcccg tcttcacgac tgggcggctg
     3181 gctgcacaaa gacatcggtt tgcccgctgt cgatgaatca attaaagaag gcagccacgg
     3241 tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt ttgtcctcga
     3301 ccgaagcgcg gccatcggtg aagagggtca ggcggcgaaa gacgtgttga ataatccgct
     3361 ggttgccgaa acaaccgcct ggaaaaaagg acaggtcgtt tacctcgttc ccgaaactta
     3421 tttggcagcc ggcggcgcgc aagagctgct gaatgcaagc aaacaggttg ccgacgcttt
     3481 taacgcggca aaataatgaa acggcggcat tcgatgccgt ctgaaacacg gatgcaaacc
     3541 gcctcctgtg tttcagacgg cattgcccga tacggaggct tcaaacaagg ctttccgctc
     3601 cgacggttcg gactgccttg tttgaatctt ctacgcctta acgcttttcc cttctgttta
     3661 tgactgccaa acctttttcc ctcaacctga ccaacctcct gctgctggcg gtattgtttg
     3721 ccgtcagcct gtcggtgggc gttgccgatt tccgctggtc ggatgtgttt tcgctatccg
     3781 acagccagca agtgatgttc atcagccgcc tgccgcgcac gtttgcgatt gtgttgacgg
     3841 gcgcgtcgat ggcggtggcg gggatgatta tgcagatttt gatgcgtaac cgttttgtcg
     3901 agccttctat ggcgggcgcg ggtcaaagcg cggctttggg tttgcttctg atgtccctgc
     3961 tgctgcctgc cgcgcctctg ccggtcaaaa tgtcggttgc cgccgttgcc gcgctaatcg
     4021 ggatgctggt gtttatgatg cttatccgcc gtctgccgcc gacggcgcaa ctgatggtgc
     4081 ctttggtcgg gattattttc ggcagcgtgg ttgaggcagt ggcgacgttt atcgcgtatg
     4141 aaaacgaaat gctgcaaatg ctcggcgtgt ggcagcaggg cgatttttcg agcgtgctgc
     4201 tggggcggta cgagctgctt tggattacgg gcggtttggc ggtgtttgcc tatctgattg
     4261 ccgaccggct gacgattttg ggactgggcg agacggtcag cgtgaatttg ggtttgaacc
     4321 ggacggcggt gttgtggtcg ggtttgatta ttgtggcttt gattacgtcg ctggttatcg
     4381 ttacggtcgg caatattccg tttgtcgggc tggtcgtgcc gaacatcatc agccgcctga
     4441 taggcgacag gctgcgccaa agcctgcctg cggtggcctt gttgggcgcg tctttggttt
     4501 tattgtgcga cattatcgga cgcgtgattg tgtttccgtt tgaaattccg gtctctacgg
     4561 tttttggtgt gttgggtacg gctttgtttt tgtggctttt gttaaggaaa cctgctcatg
     4621 ccgtctgaaa acaaaattgc atttatgcaa ggttccccgc gcccgttgtg ggcggcattc
     4681 gcactcttac taatctgctg cggactgttt ttaacgctca acgtcaaagg cgattgggat
     4741 tttgttttgc aactgcgcct gaccaaactt gccgcgctgc tgatggtcgc ctatgcggtc
     4801 ggcgtgtcca cgcaactctt ccaaacgctg accaacaatc cgattctgac cccttcgatt
     4861 ttaggtttcg attcgctgta tgtgtttttg cagaccttgc tggtgtttac gctcggcggc
     4921 gtaggctatg cttccctgcc gttgacgggt aaattcggct ttgaattggt cgttatgatg
     4981 ggcggctcgc tgctgctgtt ttacacgctc atcaaacagg gcggacgcga tttgtcgcgc
     5041 atgattttaa tcggcgtgat tttcgggatt ttgttccgca gcctgtcgtc gctgctttcg
     5101 cgcatgatcg accccgaaga atttacggcg gcgcaggcga atatgtttgc cggattcaat
     5161 accgtccaca gcgagctttt gggcataggc gcgctgattc tgctcgtcag cgtggcggcg
     5221 gtttggcgcg aacgctaccg cttggacgtt taccttttgg ggcgtgacca agccgtcaat
     5281 ttgggcatca gctacacgcg caacacctta tggatactgc tttggattgc cgcgctggtg
     5341 gcgacggcga ccgccgttgt cggcccggta agctttttcg ggcttctcgc cgcctcgctt
     5401 gccaaccact tttccccgtc cgtgcgccat tccgtccgcc tgccgatgac ggtttgtgtc
     5461 ggcggcatcc tcttggtcgg cggacagacc gtgttcgaac acttcttggg catgaaggcg
     5521 gtattaagcg tggtagtcga atttgcgggc ggactcgttt tcctctatct cgttttaaaa
     5581 cacaaaaaat gacggatgcc atctgaacgg cagcccgccc cgaaaggaca aaccatatga
     5641 cgcaagaacg tttacccgaa tttttcgacc gcgccccgac gctgaccgta caagacccgc
     5701 ttgccgcatt tctcggcgcg gcgcaagacg gcatcctcac ttaccgctac gccgatgccg
     5761 tgcgcctgtg cggacattcc tgcccgaccg tcgcgggcgc gtacctgatg gttatcaaag
     5821 gtctgaaagc cctttacggc gaagagctgc ccgaacgcgg cggcatcgaa gccgccatgc
     5881 agggaacgcg cgacgaaggc acggtcggcg taaccgcgtc cgtcgtccaa ctcctcaccg
     5941 gcgcagcccc cgaaaccggc tttggcggca tcggaataca gggacgcttc gcccgccgca
     6001 acctcttatc ctttggtgca ggcgaaatca acggcacact cgcgctccgc cgccgcgata
     6061 ccggcaaaac cgtcgccgtc agcctcaacg ccgccctgaa acccttcgca ccgcaaatgc
     6121 gcgacatcat gcccaaagcc gtcagcggca gcgcaagtac cgacgaactc aaacacttcg
     6181 gacaactctg gcaggcacgc gttaaagcat ttttgaccga atcggcggac gacccgcagt
     6241 tcgtcatcgt ccgcgaagtg tgagcgttca gacggcattc cgaatttcaa atgccgtctg
     6301 aaccccgcca aacaacaaca aacctacgcc cgacaagcat ccgccatgat taccatccgc
     6361 aacgtcagct accgcatcgg cacacgcccc atcctcgaca acgtcagcct cgacatcccc
     6421 gaaggcggca ttaccgccct cgtcggcccc aacggtgcgg gcaaatccac cctgttttcc
     6481 tttatggcgc ggctgcgccc gcttgaaagc ggcagcatcg cctaccgagg caaaaacctt
     6541 gcctgcaccc ccaccgccga actcgcccgc acgctctcca tcctcaccca agaaaacagc
     6601 atcatgagcc gcatcaccgt gcgtgacctg ctgatgttcg gccgctatcc ctaccaccaa
     6661 ggcaggccgt ctgaaaacga caaaaccatc gtcgaagaag cacttgccga gttccacctg
     6721 caagattttt ccgaccgcta cctgaccgag ctctccggcg gacaacgcca acgcgccatg
     6781 attgcgatgg tgttctgcca aagcaccgac tacgtccttt tggacgaacc gctgaacaac
     6841 ctcgatatgt atcacgcccg ctcgctcatg cagattctgc aaaagctcac gcgcgaacac
     6901 aaacgcacca ccgtcgccgt attgcacgac attaaccagg ccgccgctta cgccgaccac
     6961 gtcgtcgcca tgaaaaacgg caaagtcgcc ctaaccggca cgcccgaaga agtgttcacc
     7021 gtacacaaca tcaaagaact gttcgacatg gacgtggacg tactcgacta cgaaggcaaa
     7081 aaactcatcg tccaccacat ctgaagctca aaaagaaaat gccgtctgaa agcctttcag
     7141 acggcacccc atatcctgac aaaattaagg cacgacacag gcggcattgg cggcagcata
     7201 atatgcacat attaacagac atcaatcccg aaccgcccgc cggcaagaat ctaaataaat
     7261 aaataaataa ataaataaat aaataaataa cggaaaggta ttgcaaatat gcctcccttt
     7321 cacatatact cgaatataca ggtaaacttg gtgggaatga aaccacttgc aaaagattac
     7381 cgccccgttt ttatcctctc acaaaggtaa taagccatga aacactttca atccaaagta
     7441 ctgaccgcag ccatccttgc cgccctcagc ggcagcgcaa tggcagacaa cccccccccc
     7501 ttccactgac gaaattgcca aagccgccct ggttggagtc tacaacaata cccaagacat
     7561 caacggattc aaagtcggag acaccatcta cgacattgaa aatggccagc ctaaaggaag
     7621 accagcaaca gaagacgatg ttaaagccga tgacttcggc ggtctgggtc tgaaagaagt
     7681 cttggctcaa cacgaccaaa gccttgccga cctgaccgga accgtcgagg aaaacagcga
     7741 agcattggtg aaaaccgccg aggttgtcaa tgacatcagt gccgatgtca aagccaacac
     7801 agcagctatc agggaaaaca aagctgctat cgctacaaaa gcagacaaaa ccgaactgga
     7861 tgaagtgtcc ggcaaggtaa ccgcaaacga gactgctatc ggtaaaaaag caaacagtgc
     7921 cgacgtgtac actaaagctg aggtgtacac caaacaagag tctgacaaca gatttgtcaa
     7981 aattggtgac agaatcggta atctgaacac tactgccaat ggattggaga cacgcttggc
     8041 cgatgccgaa aaatccgtta aagaccacgg tacgcgcttg gcttctgccg aaaaatccat
     8101 taccgaacac ggtacgcgcc tgaacggttt ggatagaaca gtgtcagacc tgcgcaaaga
     8161 aacccgccaa ggccttgcag aacaagccgc gctctccggc ctgttccaac cttacaacgt
     8221 gggtcggttc aatgtaacgg ctgcagtcgg cggctacaaa tccgaatcgg cagtcgccat
     8281 cggtaccggc ttccgcttta ccgaaaactt tgccgccaaa gcaggcgtgg cagtcggcac
     8341 ttcgtccggt tcttccgcag cctaccatgt cggcgtcaat tacgagtggt aagcagcatc
     8401 tcccgataaa gaaaccgcag ccctgcaagg ctgcggtttt tattttctat ccggccgtca
     8461 aaccgctgcg tccgcacgtt cgcccgtgcg gatacggatt gcctcctcga ccggcagcac
     8521 aaaaatcttg ccgtcgccga tttttcccga acgcgccacc tcgacaatca cgtcaatcgc
     8581 gcgttccacg gcgtcatccg ccaacaccag ctcgattttg attttgggca ggaaatcgac
     8641 ggcgtattcc gcgccgcgat agatttccgt atgccccttc tgcctgccga accctttgac
     8701 ctcgctgacg gtcatgcccg taatgccgat ttccgtcaac gcctcgcgca cgtcgtcgag
     8761 tttgaacggt ttgacaatcg cctcgatttt tttcatcaca ttctccatta attaacgtaa
     8821 acaaacgaca aatatagcag aaaccgccgg caaacgataa acggtactgt tcgcccggcg
     8881 atgcggcgat ttgccggaaa ccgtcattcc cgcgcaggcg ggaatcccga tctctgcatt
     8941 tcagcaatat ttaaaggttt ctgtaaatcc aaacttccat attcctaacc gtacggaaac
     9001 gacggcattt gatctaaaaa catacggaag cagttgcccg cccctctact tcctgcaatt
     9061 atccgcatac ccggctttgg gtaaaaatca gaaaaacaga taaaaccctt attccttaat
     9121 atcttcagac ggcacctgta ttttccccaa ctgtatgccg tctgaagatt gtcaacattt
     9181 taagccaaaa tatccaagca atacaaacct gtccggcgta taatccctct ttttcccaac
     9241 cgcatttgag agccgaatcc atgtctgtcg ttttgccctt gcgcggcgtt accgcccttt
     9301 ccgatttccg tgtcgaaaaa ctcttgcaaa aagccgccgc actcggactg cccgaagtca
     9361 aattaagcag cgaattttgg tatttcgccg gcagcgagaa agcattggat gccgcgactg
     9421 tcgaaaaact gcaagccttg ttggcggcgc aaagcgttga acaaacgcca aaagcgcgcg
     9481 agggcttgca tttgtttttg gtcacgcccc gtttgggtac gatttcgccg tgggcttcca
     9541 aggcgaccaa tatcgcggaa aactgcggtt tggcaggcat cgagcgcatc gagcgcggca
     9601 tggcggtgtg gctggaaggt gcgcttaccg atgaacagaa acagcaatgg gcggctttgc
     9661 tgcacgaccg catgaccgaa tccgtattgc ccgattttca gacggcctcc aaattattcc
     9721 accatctcga atccgaaact ttctccagcg tcgatgtttt gggcggcggt aaagaggctt
     9781 tggtcaaagc caataccgaa atgggcttgg cactttcagc cgacgaaatc gattatctgg
     9841 ttgaaaacta ccaggctttg cagcgcaatc cgtccgatgt tgaattgatg atgttcgcgc
     9901 aggcaaacag cgaacactgc cgccacaaaa tcttcaacgc cgacttcatc ctcaacggcg
     9961 aaaaacaacc gaaatccctg ttcggtatga tacgcgacac gcacaacgcg catcccgaag
    10021 gcacggtcgt cgcttataaa gacaactcgt ccgtaattga aggcgcgaaa atcgagcgtt
    10081 tctatccgaa tgcggcggaa aaccaaggtt accgtttcca cgaagaagac acgcatatca
    10141 tcatgaaagt ggaaacgcac aaccacccga ccgccatcgc gccgtttgcg ggcgcggcaa
    10201 caggcgcggg cggcgaaatc cgcgacgaag gcgcaacggg caaaggttcg cgtccgaaag
    10261 caggcttgac cggctttacc gtctccaacc tcaacatccc cgacctcaaa cagccgtggg
    10321 aacaagacta cggcaagccg gaacacattt cctccccgtt ggacatcatg attgaaggcc
    10381 ccatcggcgg cgcggcgttc aacaacgaat tcggccgccc caacctcttg ggctacttcc
    10441 gcacttttga agagaagttt gacggtcagg ttcgcggcta ccacaaaccg attatgattg
    10501 ccggcggctt gggcagcatt caggcgcagc agacgcataa agacgaaatc cccgaaggcg
    10561 cgttgctgat ccaactgggc ggcccgggca tgcttatcgg cttgggcggc ggcgcggctt
    10621 cttcgatgga taccggcacg aacgacgcgt ctttagactt caattccgta caacgcggca
    10681 accccgaaat cgaacgccgc gcgcaggaag tcatcgaccg ctgctggcag ctcggcggca
    10741 aaaacccgat tatctccatc cacgacgtgg gcgcaggagg tttgtccaac gccttccccg
    10801 aactggtcaa cgatgccgga cgcggcgcgg tattcaagct gcgcgacgtg ccgctggaag
    10861 aacacggttt aacgcctttg caaatctggt gcaacgaatc gcaagagcgt tatgtgttgt
    10921 cgattttgga aaaagatttg gacaccttcc gcgccatctg cgaacgcgaa cgctgcccgt
    10981 ttgccgtggt tggcacggcg accgacgacg gccatttgaa agtgcgcgac gatttgttcg
    11041 ccaacaaccc cgtcgatttg ccgctgaacg tcttgctcgg caaaccgccc aaaaccacgc
    11101 gtaccgacaa aacggttgcg ccgtatgcga ccgaagggaa tccctttggg aaaaaaccgt
    11161 ttaacgccgg cgatatcgac attaccgaag ccgcctaccg cgttttgcgc ctgcctgccg
    11221 tagccgccaa aaacttcctg attaccatcg gcgaccgcag cgtcggcggc atgacccacc
    11281 gcgaccaaat ggtcggcaaa taccaaaccc ccgtagccga ctgcgccgtt accatgatgg
    11341 gcttcaatac ctatcgcggc gaagcgatgt ctatgggcga aaaaccgacc gttgccctgt
    11401 ttgacgcgcc tgcttcaggc agaatgtgcg tcggcgaagc catcaccaac atcgcggcgg
    11461 tcaacatcgg cgacatcggc aacatcaagc tctccgccaa ctggatggcg gcgtgcggca
    11521 acgagggcga agacgaaaaa ctctaccgca ccgtcgaagc ggtttccaaa gcctgtcagg
    11581 cattggattt gagcattccc gtgggcaaag acagcctgtc gatgaaaact gtgtggcagg
    11641 acggcgaaga gaaaaaatcc gtcgtctcgc cattgagcct gattatctct gcgttcgcgc
    11701 cggtcaaaga cgtgcgcaaa accgttacgc ccgaattgaa aaacgtcgaa gacagcgtat
    11761 tgttatttat cgatttgggc ttcggcaaag cgcgtatggg cggctctgca ttcggtcagg
    11821 tatacaacaa catgagcggc gacgcgcccg atttggacga tacaagtcgt ctgaaagcgt
    11881 tttacaacgt gattcaacag cttgtcgccg aagacaaact cttggcatac cacgaccgca
    11941 gcgacggcgg tttgtttgcc gttttggtag aaatggcgtt tgcggggcgg tgcggcttgg
    12001 atatagattt aaatttattg cttgcacaaa catttattac caaccatacc gctctgtctc
    12061 aatcattgcg gactgaagag gtaaaagcgt tggctgaatg gcaagaaacc attgcccgca
    12121 cattatttaa tgaagagttg ggtgctgtta tccaagttag aaaacaagat gttgccgata
    12181 ttatcaattt attctatcaa caacagctgc atcataatgt ctttgaaatc ggtacgttaa
    12241 ctgatgagaa cacgttaatc atccgcgacg ggcaaacgca ccttatttct gacaacctaa
    12301 tcaaactgca acaaacctgg caagaaacca gccatcaaat ccaacgcctg cgcgacaacc
    12361 ccgcctgcgc cgacagcgag ttcgcattga ttggcgacaa cggacgcagc gcattgtttg
    12421 ccaacctgaa attcgacgtg aacgaagaca tcgccgcgcc gtttatcaac agcggcgcga
    12481 aacccaaaat cgccatcctg cgcgaacagg gcgtaaacgg gcaaatcgaa atggccgccg
    12541 cctttacccg cgccggattc gatgcttacg acgtgcatat gtccgacctg atggcaggcc
    12601 gcatccacct cgccgacttc aaaatgctgg cggcgtgcgg cggcttcagc tacggcgacg
    12661 tactcggcgc gggcgaaggc tgggcgaaat cgattctgtt ccaccctgct ctgcgcgacc
    12721 agtttgccgc cttcttcgcc gacccggaca cgctgacatt gggcgtgtgc aacggctgcc
    12781 aaatggtcag caaccttgcc gaaatcatcc ccggcacggc aggctggccg aagttcaaac
    12841 gcaacctgag cgaacagttt gaagcgcgcc taagcatggt tcacgtcccc aaatccgcgt
    12901 cgctgattct gaacgaaatg caaggctcca gcctgcccgt cgtcgtcagc cacggcgaag
    12961 gccgcgccga cttcgcactt cacggcggca acatttctgc cgatttgggc attgcgctgc
    13021 aatatgtgga cggtcaaaac caagttaccc aaacctatcc gctcaacccc aacggctcgc
    13081 cgcaaggcat cgccggcatt accaacgccg acggccgcgt gaccatcatg atgccccacc
    13141 ccgaacgcgt gtaccgcgcc gcacaaatga gctggaaacc ggaaggctgg acggaactgt
    13201 ccggctggta ccgcctcttt gccggcgcac gtaaagcctt gggctaaccg ccctactcaa
    13261 accaatgccg tctgaagaat atttcagacg gcattccggc acaccatcct ttaaacggta
    13321 tccgtccacc gaggaacact catgaaaatc acacccgtca aagccctaac cgacaactac
    13381 atctggatga tacagcacgg caaccatgcc gtctgcgtcg acccttccga accctcgccc
    13441 gtcttggaat tcctcgtccg caaccgcctc atgcttgccc aaacatgggt aacgcacccc
    13501 caccccgacc acgagggcgg tgcggcggca ctctggcgcg gctacatgga atcgcccgtt
    13561 tacggcgaat ccgacatcga agcagcaacc cacaccgtaa ccgccggcac ccaattcacc
    13621 ttcggcgacg gacaggttac cgtttgggca acacccggcc acacagaccg ccacaccagc
    13681 taccttctcg aaacttcaga cggcatacac gtcttttgcg gcgacaccct gttttccgcc
    13741 ggctgcggac gcgtgtttac cggcacaatc gaacagcttt acgacagctt ccaacgcttc
    13801 aaccgcctgc ctgaaaacac cctgttctac ccggcacacg aatacaccgc cgccaacctg
    13861 gattttgccg cccatatcga gccggacaac gccgacattc agacggcact gaaagcggcg
    13921 gcgcatacgc ctaccctgcc cgttaccctc gcgcgcgaac gccgcgtcaa cccgtttttg
    13981 cgcgtcgacc tgccgcacgt cagagaccgc gccgaggcat tgagcgggaa aacgttaaac
    14041 agcagcctcg atacctttgt cgcgctgcgt gaacttaaaa accaataccg gacgaaataa
    14101 aacaacggga aaacgcagcc attcctagga tttttattaa aatcttaaat aaaatcatac
    14161 aatcatcgcc aatagacgaa aggacaccgt tgccttataa tcaaacaaaa acaaaatata
    14221 taatatagtg gattgaattt aaatcaggac aaggcgacga agccgcagat agtacggcaa
    14281 ggcgaggcaa cgctgtactg gtttttgtta atccactata ttgttaatcc actatataaa
    14341 tccagcacaa aacgggatcg gtgattcttg tccgcaagaa tcgttgattt tctctattac
    14401 acggataatc atcatgcgct tcacacacac caccccattt tgttccgtat tgtccaccct
    14461 cggtcttttt gccgtttccc ctgcttactc atccattgtc cgcaacgatg tcgattacca
    14521 atattttcgc gactttgccg aaaataaagg cgcgttcgcc gtaggtgcaa gcaatatttc
    14581 catccaagac aagcaaggca aaatattagg cagggttctc aacggcatcc ccatgcccga
    14641 cttccgcgtc agcaaccggc aaaccgccat cgccaccctg attcaccccc aatacgtcaa
    14701 cagtgtcaaa cacaacgtcg gctacggttc catacaattc ggcaacgaca cccaaaatcc
    14761 agaagaacaa gcctatacct accgcctcgt atcacgcaac ccgcacccgg actacgacta
    14821 ccaccttccc cgcctcaaca aactggttac cgaaatctca cctgccgcac tcagcagcgt
    14881 acccttgctt ggaaacggcc agccaaaggc caatgcctac ctcgataccg accgcttccc
    14941 ctactttgta cgactcggct caggcacgca acaagtccgc aaagcagacg gcacgcgcac
    15001 acaaaccgcc ccggcatacc aatacctgac cggcggcacg ccgctgaaag tattgggttt
    15061 ccaaaaccac ggcttactcg tcggcggcag cctgaccgac caacccctta acacctacgc
    15121 aatcgccgga gacagcggtt cccccctgtt tgccttcgac aagcatgaaa accgctgggt
    15181 gcttgcgggc gtactcagca cctacgccgg cttcgacagc ttcttcaaca aatacatcgt
    15241 cacgcaaccc gaattcatcc gttccaccat ccgccaatac gaaacccggc tggatgtcgg
    15301 gctgaccacc aacgaactca tatggcgcga caacggtaat ggcgacagca ccctgcaagg
    15361 gctcaacgaa cgcatcaccc tgcccattgc aaacccttcg cttgccccac aaaacgacag
    15421 caggcacatg ccgtctgaag atgccggcaa aacgctcatc ctatccagca ggttcgacaa
    15481 caaaacactg atgctggcag acaatatcaa ccaaggcgca ggcgcattgc agttcgacag
    15541 caacttcacc gtcgtcggta aaaaccacac atggcaaggt gcaggcgtta tcgtagccga
    15601 cggcaaacgc gtcttctggc aagtcagcaa ccccaaaggc gaccggctct ccaaactggg
    15661 cgcaggcacg cttatcgcca acggacaagg catcaaccag ggcgacatca gcatcgggga
    15721 aggcactgtc gtactcgccc aaaaagctgc ttcagacggc agcaaacaag cattcaacca
    15781 agtcggcatc accagcggca ggggcacggc cgtcctcgcc gacagccagc aaatcaaacc
    15841 cgaaaacctc tatttcggct tcaggggcgg acggctcgac ctcaacggca acaaccttgc
    15901 ctttacccat atccgccatg cggacggcgg cgcgcaaatc gtcaatcaca accctgacca
    15961 agccgcgaca ctgacgctga ccggcaaccc cgtcctcagt cccgagcatg tcgagtgggt
    16021 gcaatggggc aaccgtccgc aaggcaacgc ggcggtttac gaatacatca acccgcaccg
    16081 caaccgtcgg accgactact tcatactcaa acccggcggc aacccgcgcg aatttttccc
    16141 gttaaatatg gaaaactcaa caagctggca atttatcggc aacaacaggc aacaggccgc
    16201 caaacaagtc gcccaagccg aaaatgcccg ccccgaccta attaccttcg gcggatactt
    16261 gggtgagaac gcgcaaacgg gcaaagccgc accgagttac agcaaaacca atatcgaatg
    16321 ggtgaatggt ccaaacaccc cacacaagcc ctcgccctat acgaatacgc caacccgcac
    16381 aggggcaggc gtaccgacga ttaccgcctc aaagaaggcg gcaacccctg cgcctacttc
    16441 ccatggaatg cctacagcac ccaagactgg gaatttctcg gacatgaccg gcgaagcagc
    16501 catagaaaaa acccgccata tcgcaaatgc cgccgtatac ggccggcccg aataccgtta
    16561 caacggcgca ctcaacctgc actatcgtcc caaacgcacc gacagcacgc tgttgctcaa
    16621 cggcggcatg aaccttaacg gggaagtctt gattgagggc ggcaatatga ttgtgtcagg
    16681 caggcccgta ccccatgcct acgaccacca ggccaaacgc gaacccgttc ttgaaaacga
    16741 atggaccgac ggcagcttca aggctgcacg gttcaccctg cgaaaccatg cccgactgac
    16801 ggcagggcgc aataccgcgc atctggacgg cgacataacc gcatacgatc tgtccggcat
    16861 cgacctcggc tttatccaag gcaaaacacc ggaatgctac cgctcctacc atagcggcag
    16921 cacccactgc acacccaacg ccgttttaaa agccgaaaac tatcgtgcac tacctgcaac
    16981 gcaagtacgc ggcgacatta cccttaacga ccgttcagag ctccgcctgg gcaaagcaca
    17041 cctgtacggc agcatccgtg ccggcaaaga caccgcagtc cgcatggaag cagacagcaa
    17101 ctggacactt tcccagtcca gccacaccgg cgcactgacg cttgacggcg cacaaattac
    17161 cctgaacccc gatttcgcca ataatacaca caacaaccgc ttcaacacac tgaccgtcaa
    17221 cggcacactt gacgggttcg gcacattccg attcctgacc ggcatcgtcc gaaaacaaaa
    17281 tgcccccccc ctcaaactgg aaggggacag ccgcggcgca ttccaaatcc acgtcaaaaa
    17341 caccggacaa gaacctcaaa caaccgaatc gcttgcactt gtgagcctca atccgaaaca
    17401 cagccaccaa gcccgattca ccctccaaaa cggctatgcc gatttgggtg cctaccgcta
    17461 catcctccgc aaaaacaaca acggatacag cctgtacaac ccgctcaaag aggccgaact
    17521 tcaaattgaa gccacgcgtg cggaacatga gcgcaaccaa caggcataca accaattaca
    17581 ggcaaccgac atcagcagac aggttcaaca tgactctgac gcgaccaggc aggcactaca
    17641 ggcctggcag aacagtcaaa ccgaacttgc ccgcatcgac agccaagtcc aatatctgtc
    17701 cgcccaattg aaacagacag acccgctgac cggcattctg acgcgtgccc aaaacctgtg
    17761 tgccgcacaa ggatacagtg tcgatatctg ccgtcaggtt gccaaagccg ccgacacgaa
    17821 cgacctgaca ctcttcgaaa ccgaactgga tacgtatata gaacgtgtag aaatggccga
    17881 atccgaactt gacaaagcac ggcaaggcgg cgatgcgcaa gccgtcgaaa cagcccggca
    17941 cgcctacctg aacgcactca accgtctgtc ccgacaaatc cacagtttga aaaccggcgt
    18001 tgccggcatc cgtatgccga acctggccga actgatcagc cggtcggcca acaccgccgt
    18061 ttccgaacag gccgcctaca ataccggccg gcaacaggcg ggacgccgca tcgaccgcca
    18121 ccttaccgat ccgcagcagc aaaacatctg gctggaaacc ggtacgcaac aaaccgacta
    18181 ccatagcggc acacaccgtc cctaccaaca aactaccaac tatgcacata tcggcatcca
    18241 aaccggcatc accgaccgtc tcagtgtcgg tacgatttta accgatgagc gcacaaacaa
    18301 ccgttttgat gaaggcgtat ccgcccgaaa ccgcagcaac ggcgcacatc tgttcgtcaa
    18361 aggggaaaac ggcgcactct ttgccgcggc agatttaggc tacagcaaca gccgtacccg
    18421 atttaccgat tatgacgggg ctgccgtccg ccgccacgca tgggatgcag gcatcaacac
    18481 cggcatcaaa atcgataccg gcatcaacct cagaccctat gccggcatcc gtataaaccg
    18541 cagcaacggc aaccggtacg tactcgacgg cgcagagata aacagcccgg cgcaaatcca
    18601 aaccacatgg catgccggca tccgtctcga taaaaccgtc gaactgggtc aagccaagct
    18661 gacccccgct ttcagcagcg attactacca tacccgccaa aacagcggtt ccgccctcag
    18721 cgtcaacgac cgtaccttac tgcagcaagc cgcccacggc acactgcata ccctgcaaat
    18781 cgacgccgga tacaaaggct ggaacgccaa acttcatgcc gcttacggca aagacagcaa
    18841 caccgcccgc cacaaacagg caggaatcaa aataggctac aactggtaac aagccggtaa
    18901 aaatgccgtc tggaacccgc gtttcagacg gcatttgcgt taaaaatagt aaaccgtttc
    18961 aaaagggagt agaatagtgc cgtttccaac cctgcgcctg taccgtcagg cttttattat
    19021 ggaccttccc agttcgtttt tactgaacgc cccttccgat tcaaacccgc aataaccatc
    19081 ccgccggaat gcctccccgc acacggcggg cggagcattt atgagcatcg aaccaacccc
    19141 tccgaacctt gaaaacgacg gtatcgaaaa cgatgtagaa cgcgtttccg ccgatttcga
    19201 ccgtgtccac tccctctgcg aaattctcga acctgctttt gaacaaatcg aaaacggcac
    19261 accgctcgaa gacgcgccgc tgcgcgacaa gctgaccgag ctgaccgttc tcttgagcga
    19321 gctgcaccct gccgacgtgg cggcggtatt ggaatcgcta ccgccgcgcg aacgcaatat
    19381 cgtctggatt ctggtcaaac cggaagacga cggcgaagta ttgctggaag tatccgacgc
    19441 ggtgcgcgaa acactgatcg agtcgatgga caaagacgaa ttgttggcag cggtcgatga
    19501 tttggacgcg gacgaattgg cggaactggc agacgatttg ccgcaccaag tggtttacga
    19561 agcgctacaa acccgcgatg aggaagaacg cgcccaagtc aaagcggcaa tgtcctacga
    19621 agacaaccaa gtcggtgcga ttatggactt cgagttggtc agcatccgcg ccgatgtcgc
    19681 ctgtgaagtg gtgctgcgct atctgcgccg cttcgacagc ctgcccgacc ataccgacaa
    19741 gatttttgtg gtcgatgaaa acgacgtact gcaaggcgtg ctgcccatcc gcaaactttt
    19801 ggtcgccgat cccgaagatt tggtggaaaa cgtgatggcg aaagatgtcg tgcgtttccg
    19861 cgccgaagac gacgtggaag aagcggcgca ggcgtttgaa cgctacgact tggtaactgc
    19921 gccggttgtc gatgaaaaca aaaagctcat cggtaggatt accgttgacg agatggtgga
    19981 cgtaatccgt gaagaatccg aagcggatat gttgaacatg gcaggtttgc aggaagagga
    20041 agacctgttc gcccccgtct tggattccgt caaaaaccgc tggatgtggc tcgccgtcaa
    20101 cctctgcacc gccttcctcg ccagccgcgt catcggcgcg tttgaaggca gtatcgaaaa
    20161 aatcgttgca ctcgccgcgc tgatgcccat cgtcgccggt atcggcggca actcgggcaa
    20221 ccagacgatt accatgattg tccgcgccat ggcgatgggg caactgacag gcatgcaggc
    20281 aggcaggctg ttgaaaaaag aagtcggcgt tgccctagtc aacggcatta tctggggaac
    20341 cgtcatgggc gcggtttcat ggctgctcta cggcagcctc ggcatcgggc tggttatgat
    20401 tgccgcgatg acgctcaacc tgctgctggc ggcaaccgtc ggcgtattga ttcccgtggt
    20461 aatggaaaag ttcggacgcg accccgcgct gggcagctcg gtgctgatta cggcggtaac
    20521 ggattccggc ggcttcctga ttttcttggg tctcgccacc ctattcctgc tttaaatgcc
    20581 gtctgaaccc gcgcaaaaat gccgtctgaa gcggaagctg cttcagacgg catttgacta
    20641 tttatccttg ttgcacaaga ttattggacg gtatgccggg gcagcccttt ggcaacgccg
    20701 accacatcct ccccgaacag cgcgttgaca tcggtttcgt caaacacata tttgctgtgg
    20761 cagaaatcgc aatcgacttc gatgctgcct tgttccgcca ccacgccgcc gacttcttcc
    20821 ccgcccagca tcaacagcat atcgctgact ttgccgcgcg aacaggtgca tgaaaattca
    20881 aacgtttccg gctcgaacac gcgcggcggc gtttcgtgga acaggcggta taaaacgtgt
    20941 tgcgcgtcca attctgccag ctcctccgcc gtcagcgtgc gcgccagcgt actgacgtgt
    21001 tcccatgcct cttcatccaa cacctcttca ggcagacgct gcaccagcag accgcccgcc
    21061 gcttcgtcgc ttgcagacag gacgatgtgc gtatcaagct gttcggaacg tttcatatag
    21121 ttcatcaaca tttgcgcgat accgtcgcct tccaaaggca ctacgccctg ccagggttcg
    21181 ccgtctttgg gctgcagcgt caatacgaac acgccgttgc cgcccaaaag gtcgccgagg
    21241 ctttcgtcat cggctatttc tgccgcctcg tcccaccgcg cggttgcacg gacggtacga
    21301 tcggaagtcg cttccgcaac cagcattttc agccgcccct gcccctgaac ctgcacaatc
    21361 agcgtgcctt cgtttttgag gttgcccgac agcagaacac cggcagccaa cagctcgccc
    21421 aaggcgcggc ggatggcggc gggatagttt ttctgtttta caatgtgctg ccacacgttt
    21481 tccagacgga cgtgcagccc gcgcacgggc atatcgtcga agataaagcg ggtacgcaca
    21541 tcggcgcggt tgatggcggt ttgattcatg attttctctg actgattgtt cggatggcgg
    21601 ctatatggtt gcggtcggcg cgaaaacaag acggacggcg gatgcgcttc ccaaattatc
    21661 aataaattat ataaaaatca acatattaac tcaatctaac aagccgtttt ttgccaaaca
    21721 gccgtttttt tatatacaat caacaagata ttttcgactg atacagcata acatcgcacg
    21781 gcggcacgat gcctcctgcg cggaaacacc gatatggatt cttttttcaa accggcagtt
    21841 tgggcggttt tgtggctgat gtttgccgtc cgccccgccc ttgccgacga gttgaccaac
    21901 ctgctcagca gccgcgagca gattctcaga cagtttgccg aagacgaaca gcccgtttta
    21961 cccatcaacc gagcccccgc ccgacgggcg ggcaatgccg acgaactcat cggcagcgcg
    22021 atggggctta acgaacagcc cgttttaccc gtcaaccgag tccccgcccg gcgggcgggc
    22081 aatgccgacg aactcatcgg caacgcgatg gggcttaacg aacagcccgt tttacccgtc
    22141 aaccgagccc ccgcccggcg ggcgggcaat gccgacgaac tcatcggcaa cgcgatggga
    22201 cttttgggta ttgcctaccg ctacggcggc acatcgattt ctaccggttt tgactgcagc
    22261 ggcttcatgc agcacatctt caaacgcgcc atgggcatca acctgccgcg cacgtcggca
    22321 gaacaggcgc ggatgggtac gccggttgcc cgaagcgaat tgcagcccgg ggatatggtg
    22381 tttttccgca cgctcggcgg cagccgcatt tcccatgtcg gactttatat cggcaacaac
    22441 cgcttcatcc acgcgccgcg cacggggaaa aatatcgaaa tcaccagcct gagccacaaa
    22501 tattggagcg gcaaatacgc gttcgcccgc cgggtcaaga aaaacgaccc gtcccgcttt
    22561 ctgaactgat ttcccaagga atacgcaatg agtatgcccg aaatgcccaa atggtacgac
    22621 gatgacggac agatcgtgtc ctgtaccgaa aaggtcaaag tgatgtccga aaatatggcc
    22681 gagctgtatc agacggcaca agacgcgttt gaagacgcgc tgctgatggg ttgcggcgaa
    22741 cgtcagttgc gcgattacct gctcgcgctg attgaaggtt tggaaaatcc ctaccgcaaa
    22801 gtctgaacac gccccggttg ctgcggcacg gtttatccgt gccgtttttg cgtttgtgcg
    22861 cggcttcggc ttttcagacg gcatatttga cgttatgatt aaacagttaa caagatttat
    22921 cacaacgccg tcaaaagaca gacacacaac atgaacatca tccgcgcgct cctcatcatc
    22981 ctcggctgcc tcgccgccgg cgaaaccgcc gttttcctag caggcatcaa actgcccggc
    23041 agcatcgtcg gcatgggcgt gctgtttgcg cttttgcagg cgggttgggt caaaacgtct
    23101 tggctgcaac agcttaccga cgcgctgatg gcgaatctga cgctgtttct cgtgccgccc
    23161 tgcgtggcgg tcatcagcta tttggatttg attgccgacg attggttttc gatactggtt
    23221 tccgcctccg ccagcacttt gtgcgtactg ctggttacgg gcaaagtcca ccgctggata
    23281 cggagcatta tctgatgaac gaaatcctca ggcagcccag cgttctgctt ttcctcacgc
    23341 ttgccgtgta cgcgcttgcg attatcgtgc gcacgcgcac gggcaatatc ttctgcaacc
    23401 ccgtactcgt cagcaccgtg gtgctgattg cctacctgaa aatcctcgac atcgattatg
    23461 cggtgtacca caacgccgcg caattcattg atttttggct gaaacccgcc gtcgtcgtgc
    23521 ttgccgtgcc gctctaccaa aaccgccgta aaatcttcaa ccagtggctg cccgtcatcg
    23581 tttcacagct tgcgggcagc gttacgggca ttgttacggg gatgtatttt gccaaatggc
    23641 tgggcgcgga acgcgaagtc gtcctctcgc tcgcgtccaa atctgttacc aaccccatcg
    23701 ccattgaaat tacccgctcc atcggcggca ttcccgccat taccgccgcc accgtcatca
    23761 ttgccggtct ggtcggacag attgccggtt acaaaatgct gaagaacaca gtcgtcatgc
    23821 cctcgtccgt gggtatgtcg ctcggcacgg cttcgcacgc gatggggatt gccgcctcgc
    23881 tcgaacgcag ccgccgtatg gcggcatacg cgggactggg gctgacgttc aacggcgtac
    23941 tgaccgcgct gattgcgccg ctgctcatcc ccgttttggg attttgaacc cgtttcagac
    24001 ggcatttcag cccatgctgt ctgaacgccg acacactcgc aaggagaacc gttatggctg
    24061 tcaacctgac cgaaaaaacc gccgaacaac tgcccgacat cgacggcatt gccctctaca
    24121 ccgcccaagc aggcgtgaag aagcccgggc ataccgacct gacactgatt gccgtagccg
    24181 ccggcagcac cgtcggtgca gtcttcacga ccaaccgttt ctgtgccgcg cccgtccaca
    24241 tcgccaaatc gcaccttttc aacgaagacg gcgtgcgcgc cctcatcatc aacacgggca
    24301 acgccaacgc gggtacgggc gcacagggca gaatcgatgc tttggcagtg tgtgccgccg
    24361 ccgcccggca aatcggctgc aaaccgaacc aggtgctgcc cttctccacc ggcgtgattc
    24421 tcgaaccgct gcccgcagac aaaatcatcg ccgcgctgcc caaaatgcag cctgccttct
    24481 ggaacgaagc ggcacgcgcc atcatgacca ccgacaccgt gcccaaagcc gcctcgcgcg
    24541 aaggcaaggt cggcgacaaa cacaccgtcc gcgccacggg catcgccaaa ggctcgggca
    24601 tgattcatcc caatatggcg accatgctcg gtttcatcgc caccgatgcc aaagtttccc
    24661 aacccgtcct ccaactgatg acgcaggaaa tcgccgacga aaccttcaac accatcaccg
    24721 ttgacggcga caccagcacc aacgacagct tcgtcatcat cgccaccggc aaaaacagcc
    24781 aaagcgaaat cgacaacatc gccgacccgc gttacgccca actcaaagaa ttgttgtgca
    24841 gcctcgcgct cgaactcgcc caagccatcg tccgcgacgg cgaaggtgcg accaagttca
    24901 tcaccgtccg cgtcgaaaac gccaaaaccc gcgacgaagc ccgccaagcc gcctacgccg
    24961 tggcacgttc gccgctggtc aaaaccgcct ttttcgcctc cgaccccaac ctcggcaggc
    25021 tgctcgccgc catcggttat gccggcgttg tcgacctcga tactgacctc gtggaaatgt
    25081 atctcgacga tattttggtt gccgaacacg gtggacgcgc cgcaagctac accgaagcac
    25141 aagggcaggc ggtgatgtcg aaggccgaaa tcaccgtccg catcaagctg catcgcggac
    25201 aaagtgccgc caccgtctat acctgcgacc tgtcgcacgg atacgtttcc atcaacgccg
    25261 actaccgttc ctgacccgac acggcttcag acggcataca taaaatgccg tctgaaccgc
    25321 cggacaacat gccatgacct ccacattccc ccgccgcctc gcccgcaaaa tccgccaaac
    25381 ccgccgcctg tcgcgcaaaa gcatcgcctt tctgttcctt ttggcaggtt cggcactcgt
    25441 cgccctgacc gcgctgtttt ttgcccatct tgccgatttt gcgctggaac tgaacgccaa
    25501 actggttcaa caatacccgt ggttcgcgtg ggtcgcgctt cctttgggtt tgccgcttat
    25561 tgcgtggctc acacgcaaat tcgccccctt caccgccggc agcggcatcc cgcaggtcat
    25621 cgcctcactg tcgctgccct acggcgcaca gaaaacgcgg ctgatccgcc tcgggcagac
    25681 gctgctgaag attccgctga cctttttggg tatgctgttc ggcgcgtcca tcggacgcga
    25741 aggtccgtcc gtgcaggtcg gcgcggcagt gatgggcgcg tggggcgcgt ggtgcaagaa
    25801 acacggcttg gcattcaaag ggatgcagga aaacgatttg atggcggcgg gcgcggcggg
    25861 cggtttggca gccgcgttca acgcgccgct ggcgggcgtg attttcgcca ttgaggaact
    25921 cgggcgcggc atcatgttgc gctgggagag gcaaattctt ttgggcgtgc tcgcctccgg
    25981 tttcatacag gtcgccattc agggcaacaa cccgtatttt tccggcttca acggcggcgt
    26041 attggaacat atctttctgt gggtcgcact gtccggcctg gtttgcggcg cggcgggcgg
    26101 gctgttcgga cgtttgctct atcgcggtgc ggcggcgttt gcaccgcgca agatacgcgg
    26161 cttcatccgc aaccgtccgc tgctgctggc ggcactgatg gggctgctgc tcgccctgct
    26221 cggcacgttc taccaaggca aaacctacgg caccggctac cacgaagccg cccaagccct
    26281 gcacggcatc tacgaagccc ccttcggact cgccgccgcc aaatggctcg ccaccgtatt
    26341 cagctattgg gcaggcattc cgggcggcat tttcactccc tcgctgacca taggcgcggt
    26401 tttgggcgag catatcgccg ccatcgccga catatcgcag ggtgcaaaca tcatcgtcct
    26461 catctgcatg gcggcatttc tggcgggcgc gacacaatcc ccgattactt ccgccgtcgt
    26521 cgtcatggaa atgacgggcg gacaaagcct gctgttttgg atgctaattg cctgcatttt
    26581 cgcctcgcag gtttcgcgcc agttttcgcc gcgtccgttc taccacgcat cgggaatgcg
    26641 cttccgccag cgcgtgcttc aagaaaccgc cgcccaaacc ggcaatgcgc ccgcaagacc
    26701 gcaaacagca aacagcaaaa cgggaatgcc gtctgaaaat taaaacgccc ccgatcaaac
    26761 gccggcagcc gccttgattt gaataccgtt ccgccgccgc ttgaaatttc agcaacaatg
    26821 ccgtctgaac gacagaatgc ggttttcaga cggcatttcc acatcccgat attgcctaaa
    26881 caaaaccgaa gcgtttgcta taattctatt ttttaccgca cacgcaccaa tcatgtttcc
    26941 cgatttctcc caaaccctct ccaaagaccg ccacttccta cgttccgcct tcaaaaatcc
    27001 caacaaatac ggcggcttgt ccaaagtcga agaaaaatac caaaaatcgc acgaaatctt
    27061 tttgaagcgt ttggctgcct tgcccaaacc cgaattcgat aacaccctgc ccgttcacga
    27121 gaagctcgac gaaatcaaaa aagccattgc cgagaatcag gtaacgatta tttgcggcga
    27181 aaccggttcg ggcaagacca ctcagttgcc caagatttgt ctggaactcg ggcgcggggc
    27241 ggcgggtttg atcgggcata cccagccgcg ccgtttggcg gcgcgttccg tagcagagcg
    27301 gattgccgaa gagctgaaat ccgaaatcgg cagcgcggtc ggctataaag tgcgcttcac
    27361 cgaccacacc tcgcgcgatg cctgcgtcaa gctgatgacc gacggcatcc tgctggcgga
    27421 aacccagacc gaccgttatc tcgccgccta cgacacgatt atcatcgacg aagcgcacga
    27481 gcgcagcctg aacatcgact tccttttggg ctatttgaaa caactcctgc cgcgccgccc
    27541 cgatttgaaa gtcatcatca cctcggcaac gatagacgca gaacgctttt cccaacactt
    27601 caacggcgcg cccgtgctgg aagtgagcgg acgcacctat cccgtcgaaa tcctctaccg
    27661 accgctgatc ggcaaagacg aagacgacgc ggaaatagaa ttgaccgacg cgattgtcga
    27721 tgcggcggac gaattggcgc gatatggcga aggcgatatt ttggtattcc tgccgggcga
    27781 acgcgaaatc cgcgaagccg ccgaagccct gcgcaaatcc accctgcgcc gtaacgacga
    27841 aatcctgccc ctgttcgcac gcctgtcgca cgccgaacag cacaaaatct tccacccctc
    27901 aggcgcgaaa cgccgcatcg tattggcaac caacgtcgcc gaaacctcgc ttaccgtgcc
    27961 gggcatcaaa tacgtcatcg acaccggtct cgcgcgcgtc aaacgctatt ccgcacgggc
    28021 gaaagtggag cagcttcatg tcgaaaaaat ctcccaagcc gccgcccgcc aacgctccgg
    28081 ccgctgcgga cgcgtatccg caggcgtatg tatccgactg ttttcagaag aagatttcaa
    28141 cagtcgcccc gaatttaccg accccgaaat cgtccgcagc aacctcgccg ccgtcatcct
    28201 gcgcatggca tcgctgaaac tcggcgacgt agcggcattc ccatttttag aaatgcccga
    28261 ttcgaggtat attaatgatg ggtttcaggt gttgctggaa ttaggggcag tggaggccgc
    28321 ctgaaaacaa gtaggatata cacttctacc tgacaaagac caacaaacga taaaactacg
    28381 gcacatctag atgcccaacc tacaacattg cacacggtaa aaacatgaaa cacttactca
    28441 tcgattttga aaacgtccag ccgcaaaact tagacaaatt actaaccgaa aatacccata
    28501 tttggctatt tataggtgta ttacacaaaa tgttacctat tagtctggtg caatccctac
    28561 tacgtttcgg cgaacgtgtc catcttgtcc agttacaaaa aacggggaaa aacgcattgg
    28621 atttttacct gtcctattac ctcggacaaa ttaccgccac agaccccaat gcccaaatcg
    28681 gcatactctc gcgtgatgga ggatacgatg ttctggtcga acatattttg aaaaaccacc
    28741 aggcgaaggg tatcgtgcgc ctagccaata tagatgaagt acaacatcag aaaattgcta
    28801 ccgaaccgcc gtcagcattg cgggaaaaca ctcctcagcc tgaaaccacc ctcaaaccac
    28861 agcaaccatt aacttcctat ttccaagcag ccctaactgc actgcgccgc cccgacgctt
    28921 tccgcccctg ccgcctgcat aacctgcgac aaaatctgcg taagcatatt ttgagtgatt
    28981 tgtttaaaga aaaaaccgat gaagaatgcg aaataaccac tgctaacgtt atcaataaac
    29041 tcaaagcaca aaacttcatc agcattgatg aacaggaaac cgtttcctac catctcagtg
    29101 ataatgattt gttacaaaga atccaacgcc atattttaag ccaacgtccc aaaacctacg
    29161 ctgattttca agccgtcgtg caaaaccgag cagatgcact tcacttaaca gtcggtacca
    29221 acgacattca atcctttgcg cgacatttgc gcgaccaaaa cctgatccgc caaaacaatg
    29281 ggaaaattga atatgcaccg tttactgaac ctaaaccaca gccaacgccc aagcagccta
    29341 aaaaaaccgc atgggaacct gatgaaatta tttggaaaaa agtgattgcc gcgttatcgt
    29401 taaagaaccg tcctaataaa accaaaactt tacgcaatac aatccaggca ctcacaaaat
    29461 ccaatgcaca agaaactgac aaactgctac aacatttaca agatacccaa gtcctacgta
    29521 ttgacggtac taaaattgtg tatatcaagt aggatgggca tttatgcctg acctacaaaa
    29581 ccaaaaacaa cctaacaccc ttaatcaact ccacaaaccc ttttcagaca acctcgtttt
    29641 ttgaaaaaca atctgtaaac agataactgc tgaagaatac cgttgccgag ccccaaaacc
    29701 tgcctgcaac ttttattgtg aacttcccat tatgaaaaaa tcccttttcg ttctctttct
    29761 ctattcatct ctacttaccg ccagcgaaat cgcctatcgc tttgtattcg gaattgaaac
    29821 cttaccggct gcaaaaatgg cagaaacgtt tgcgctgaca tttatgattg ctgcgctgta
    29881 tctgtttgcg cgttataagg cttcgcggct gctgattgcg gtgtttttcg cgttcagcat
    29941 tattgccaac aatgtacatt atgcggttta tcaaagttgg atgacgggca tcaattattg
    30001 gctgatgctg aaagagatta ccgaagttgg cggcgcgggg gcgtcgatgt tggataagtt
    30061 gtggctgcct gcgttgtggg gcatgttgga agtcatgttg ttttgcagcc ttgccaagtt
    30121 ccgccgtaag acgcattttt ctgccgatat actgtttgcc ttcctaatgc tgatgatttt
    30181 cgtgcgttcg ttcgacacga aacaagagca cggtatttcg cccaaaccga catacagccg
    30241 tatcaaagcc aattatttca gcttcggtta ttttgtcgga cgcgtattgc cgtatcagtt
    30301 atttgattta agcaaaatcc ctgtgttcaa acagtctgcg ccaagcaaaa tcgggcaggg
    30361 cagtgttcaa aatatcgtcc tgattatggg cgaaagcgaa agcgcggcgc atttgaaatt
    30421 gtttggctac gggcgcgaaa cttcgccgtt tttgacccag ctttcgcaag ccgattttaa
    30481 gccgattgtg aaacaaagtt attccgcagg ctttatgacg gcagtatccc tgcccagttt
    30541 ctttaacgtc ataccgcatg ccaacggctt ggaacaaatc agcggcggcg ataccaatat
    30601 gttccgcctc gccaaagagc agggctatga gacgtatttt tacagtgctc aggctgaaaa
    30661 ccaaatggca attttgaact taatcggtaa gaaatggata gaccatctga ttcagccgac
    30721 gcagcttggc tacggcaacg gcgacaatat gcccgatgag aagctgctgc cgctgttcga
    30781 caaaatcaat ttgcagcagg gcaggcattt tatcgtgttg caccaacgcg gttcgcacgc
    30841 cccatacggc gcattgttgc agcctcaaga taaagtattc ggcgaagccg atattgtgga
    30901 taagtacgac aacaccatcc acaaaaccga ccaaatgatt caaaccgtat tcgagcagct
    30961 gcaaaagcag cctgacggca actggctgtt tgcctatacc tccgatcatg gccagtatgt
    31021 tcgccaagat atctacaatc aaggcacggt gcagcccgac agctatctcg tgccgctggt
    31081 gttgtacagc ccggataagg ccgtgcaaca ggctgccaac caggcttttg cgccttgcga
    31141 gattgccttc catcagcagc tttcaacgtt cctgattcac acgttgggct acgatatgcc
    31201 ggtttcaggt tgtcgcgaag gctcggtaac gggcaacctg attacgggtg atgcaggcag
    31261 cttgaacatt cgcgacggca aggcggaata tgtttatccg caatgagtgg cgtaaaaacc
    31321 aataaagaca aatttagatg atgtcgggga agatgcccga ccgacaagac tatgcaaaat
    31381 atgaaaaacc aagtacgcgg atcaggcatg gatgcacgat ccaatccggc caatgtttca
    31441 gacggcctgc aaaacagttc gggtcatatc ggtaccaaca cgcgttaccg cctgaccaaa
    31501 ctcggcgaac aaatggcgcg cctgcccatc gacccgaaaa tcgcgcgcat tttgttggcg
    31561 gcgaagaagc acgactgcat ggcggaaata ttggtgatcg cgtccgcgct gtcgattcaa
    31621 gacccgcgcg agcggccgct ggaagcgcgc gatgccgccg ccaaggcgca cgagcgtttt
    31681 accgacaagc aatctgactt tcttgcctat ctgaacattt gggacagctt ccagcgcgag
    31741 cgcgacaagg gcttgtccaa caagcagttg gtgcagtggt gccgtcaata tttcctgtcg
    31801 cacctgcgga tgcgcgagtg gcgcgagctg caccaccagc ttgcccaaac cgcgattgaa
    31861 atgggtttga ccaccaagga agcggctttc agacgacttt ccgaaatcaa gcagcttacg
    31921 tcgtctgaaa accaaggcga tcaagactta tccgccaaac gcaaacaaaa acaactggat
    31981 aagaaacaac accgcgccca aatccgcgcc gccaaagaag cgggctacga acaaatccac
    32041 cgcgccctgc tcaccggcct tatcgccaac gtcggcatga aatcgcccga cggcaacgac
    32101 tataccggcg cgcgcggcag ccgtttccac cttttccccg cctccgccct gttcaaagcc
    32161 aaacccaaat gggtgatggc ggcagaactc gttgaaacga caaaacttta tgcgcgcgac
    32221 gtcgccgtta tccagcccga atggattgag caggaagccc cgcacctcgt ccgctaccac
    32281 tatttcgagc cgcactggga acaaaaacgc ggcgaagtcg tcgccagcga acgcgtgacg
    32341 ctttacggcc tgaccgtatt gccgcgccgc cccgtgcctt acggcaaagt tacgcccgaa
    32401 gaagcacgtg aaatctttat ccgcagcgcg ttggtggcgc aggaatgcga tttgaaagcg
    32461 gatttttttg tccacaataa aaagctgatt aaagaaatta ccgaactcga acacaaatcg
    32521 cgcaagcaag acgtattggt cgacgacgaa gccctgtttg cgttttatca cgaacggctg
    32581 cccgattttt atacggcgga tgcggtttca gacggtctgc gtcctgcaaa tcccca
//
LOCUS       DAXHRI010000006        31108 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXHRI010000006 DAXHRI010000000
VERSION     DAXHRI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.006"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            377..508
                     /locus_tag="ACFM75_000197"
     CDS             377..508
                     /locus_tag="ACFM75_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254805.1"
                     /translation="MQNQNTRPVKIELKGEAGKRVLLAAARRIAKTHQKAVKALADK"
     gene            505..873
                     /locus_tag="ACFM75_000198"
     CDS             505..873
                     /locus_tag="ACFM75_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFC6254806.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
     gene            889..1059
                     /locus_tag="ACFM75_000199"
     CDS             889..1059
                     /locus_tag="ACFM75_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254807.1"
                     /translation="MPSEIQTAFLLKGFSSTALHKGGFFMPSESPSDGIGLHGRSSPA
                     FKQERMSRMPDF"
     gene            1311..1802
                     /locus_tag="ACFM75_000200"
     CDS             1311..1802
                     /locus_tag="ACFM75_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC6254808.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            2161..2397
                     /locus_tag="ACFM75_000201"
     CDS             2161..2397
                     /locus_tag="ACFM75_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFC6254809.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDAGNEVV"
     gene            2399..2746
                     /locus_tag="ACFM75_000202"
     CDS             2399..2746
                     /locus_tag="ACFM75_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6254810.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(2799..3308)
                     /locus_tag="ACFM75_000203"
     CDS             complement(2799..3308)
                     /locus_tag="ACFM75_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254811.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWRFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRSRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(3320..3793)
                     /locus_tag="ACFM75_000204"
     CDS             complement(3320..3793)
                     /locus_tag="ACFM75_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HFC6254812.1"
                     /translation="MQVTKNFSLRELTRSETARRAGVPNEPSPAEMDNIYYTAQQLEK
                     IREYVGRPIIVTSCLRSERVNKLVGGSPTSAHRHGLAADCDASGMTSLAFAKLLIKMR
                     DEGALKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKTVYLPGLHP"
     gene            3941..4321
                     /locus_tag="ACFM75_000205"
     CDS             3941..4321
                     /locus_tag="ACFM75_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6254813.1"
                     /translation="MPLKFQPQERSVVMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(4526..4675)
                     /locus_tag="ACFM75_000206"
     CDS             complement(4526..4675)
                     /locus_tag="ACFM75_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254814.1"
                     /translation="MTAKEFCEKQIAYWLNERRKASDNDDVKAFEFAERELANYREML
                     KRYAV"
     gene            complement(4678..5352)
                     /locus_tag="ACFM75_000207"
     CDS             complement(4678..5352)
                     /locus_tag="ACFM75_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254815.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWRDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYATGEFAAGYVYEIDSIRFVFDGKSVCLKLVSPETADV
                     CAPGWRDGAATGLPVYAVQGDEKLTLAPAPDRNGRLFVGGYCLPRDMEEDGDAPEINS
                     IHHRNLVYWALAEAFSIPDTETFDPQRSESARRRFELYFGLPTDSDLRRITREDTLHL
                     NRHFWI"
     gene            complement(5349..5756)
                     /locus_tag="ACFM75_000208"
     CDS             complement(5349..5756)
                     /locus_tag="ACFM75_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254816.1"
                     /translation="MDRVFIKYIGGRAVWRDGIYHTGLVFEDGQVREVSAEAAAKLLR
                     HGDVFVAAPGKRVIKADDTEALEKAGALEVEREEAAFDAVQDVILQINRMGKDELELY
                     AKANYGQSLDKRKSAENLREAVVQMVHQFGIVQ"
     gene            complement(5840..6313)
                     /locus_tag="ACFM75_000209"
     CDS             complement(5840..6313)
                     /locus_tag="ACFM75_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254817.1"
                     /translation="MAKIHVRNNGGNRFGGVPYGNMAVEHYRIVAKEDGTILGADAYG
                     PPKADDVLVLGVLEQGFRLDDAQIIVKTGMASGVTADVGFVYADGADDANVPQDAAYF
                     ASAADFASAARIRCQSAKLVTLPKQALLTVTLKGADSKAAADIDILIYGEKFGQL"
     gene            complement(6371..7666)
                     /locus_tag="ACFM75_000210"
     CDS             complement(6371..7666)
                     /locus_tag="ACFM75_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFC6254818.1"
                     /translation="MTQKTNMAYGDPQAMMKQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPIVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGKGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALMRPVAQSLMDRYADQTLLVHMAGAR
                     GFHNNIEWGVPLAGDPKFNDYVVNPVKAPSKNRHFVASGGAVTGVAAKGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAASDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGEAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELDHGNRVETLIGT
                     IRGVAKTRFAVDVGEGEKEITDYGATVVDTVVPLQGGIR"
     gene            complement(7687..8610)
                     /locus_tag="ACFM75_000211"
     CDS             complement(7687..8610)
                     /locus_tag="ACFM75_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254819.1"
                     /translation="MEDEIDYEDLGLILTPEQLAVLEADDGAESAGDTDADGEKAPDA
                     EDAADAPDGGKPETSESDADAREDGSDGGVPDDDADKVIRAKDGKHTIPYEKLVEARE
                     AEKSAKAEAEALRKRIAQLEKGAEKPEVKTADDSGNSLFGDFSDEDMKKGVEKLIQEK
                     LAGYEANLKQQEAAKAHYREIYTAHPDADSIVESRELEEWLAGQNPLVRKAFHDALKD
                     GTAAEVIGAFDLFKAAKSAAEPEKPDEKPDEKPDEKPPVRKNTPNTLSDIPAGRDHTA
                     SDSPLDHLSGNALAEKLASMTEEQVEKFLNS"
     gene            complement(8695..9888)
                     /locus_tag="ACFM75_000212"
     CDS             complement(8695..9888)
                     /locus_tag="ACFM75_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254820.1"
                     /translation="MAEVGITPVAGVNNVARDDDMVRGPDAPGRFVRDAVNVDIHADG
                     AFSMRGAARKISGLKLRDVWQSPLHRDVFCVHEGHLCKLDTGDFSVSVLVRDAGAGRV
                     WYAVVNHAVFAATDRGIFVYDGLSAQRLGIDTPPAPLVMPSEGSLAAGHYGFALAWLR
                     GGKEGGLSAMASVAVADGGGVEVALPFCTDRTVDGVRLYATAADGGELACIGDYPVSG
                     TIVLPLLPKQGKTAQMRFLSPMPTGKFMCVWRGRLLTARANVLRFSEPLAFHLHDGRF
                     GYMQMPQRITFVEPVEGGIFVGQVDGVVFLRGVDVEALEMVRLAVKPPVAMSSVRLDA
                     DEAGQFSNGAAVCLWLADNGAAVGLPDGGVVEVHAGVLRGLAAGTAAQTVSADRRFVS
                     LMLDS"
     gene            complement(9876..13091)
                     /locus_tag="ACFM75_000213"
     CDS             complement(9876..13091)
                     /locus_tag="ACFM75_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254821.1"
                     /translation="MDRINPHGLPLVFGYVRRDGDGVGPLPFGDGGGRFFVWFKLGDT
                     SAFGRASVWNWETPVFDAQASDGVERFGAAVFISNLPFYPVGFAPSVFGDRSKIYNRD
                     FLVVSEGWDSFYQGEGAAKSLGRFRRHLVVERVLPLVFALPLSAKNAKTELPFGFKSE
                     VSYLFFPGLYGSDAGTPRVYSTIQDVRRVSLGDVSAAGGAVSVRNAGQRLRQRPHGDD
                     LAFPFPIVFNRTYRVLATVENRFAWGVARVFDPLQKVGRGADFDSLVFGQAWFSHFLR
                     HIEIGQGVIGGFFEQRRTVRVSFGRQYVVLPANSFRRRQFSRNHTVGFPPEIRPLGWD
                     SAAYLTRIVPPGQAVRVDAGMRVRVSFGVSSVRQSVLRVSAWSIPPWVNNWAHILRNV
                     RQYVRPSDQVQEQIDGMVFGAAYPYNRDRVVRPSRFAAVRWGYTVLLLAGERVVPATL
                     PPPKPGKPSVTNRVRYAAPSGWDGARIPTGAAVWTNGQVLLPPGTDMAVHTRGAYVWK
                     NRRFLRRFRGIEGEAYGTAFAAYAVRHIFQDDGSLSFMIRPPDMRMPSVALLKRYVPV
                     RGTDSAAFGTGVFRIRRNMIAPSWPPDDGGRDFGLAYLKKRNAEVYLHGFGGFAFSDG
                     AAVQYWVRTLGVETFNRHTLGRLTWIGRRVRFIGMGGIGTMRVPDGHDVWTDSTPPFG
                     TKLILLDAWSSEDGASSGSGNGWDSVLFGGTGLTDMSVRDAGGFAPSLFSIGTRFFNN
                     GILVRQGIPVGTAGRCAVSHRHRPIVAKGWRMDFALGRPRVTPHTVWVMYSPPPKAAE
                     NNGGGGYHAPSEGIRTGYPYIVFNSGRVEQYAAYPQYRAGKVSGYCLIENARQSVRAK
                     GIFHFRAGWNTFYPYTQYVELFSQGDVNADYMAFGVLKAGFLDVFDWRIAAAGVRFAG
                     FGDQTVRNRNQDVRPAGADMLEAGKSRWNDTPFMWQGLRVGERIPTAIGGGDLLRFGG
                     FGSVSYRNRTVRAEGMDMFASEGEARRFDDRMTVRRASGMPPDNRVGRKGEDARPVQF
                     VGVPPFGFAPFGRCDIGNKVRFIRPDGYADQFRKGASVWQR"
     gene            complement(13094..13486)
                     /locus_tag="ACFM75_000214"
     CDS             complement(13094..13486)
                     /locus_tag="ACFM75_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254822.1"
                     /translation="MADNPADNPAVGLLGFSRGGVLILKRHERKDVPFVGMGGRGLLA
                     ANHYQFGDGLIAGAGRGVVTVEGAPASRRVYLFALPDMVCIDDVWADDNGEYRFSQLD
                     TEKRFLVLARDHTGKYEPVSADNVKPEK"
     gene            complement(13486..14385)
                     /locus_tag="ACFM75_000215"
     CDS             complement(13486..14385)
                     /locus_tag="ACFM75_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254823.1"
                     /translation="MFKKSRVPVRVFRSSDKGAPQNVVGASDNAFKILNACLVEGYGD
                     TASLGWELKFKVGSTGIFMPTDKKQTGWGLRISQSEKYGARVSAVRNPSSDDTFELES
                     AYENNEAYFLHDNTNGDTKTRDWTLVGHSLGFILIFHVTEHSSDGPFNTTLYFGRVPT
                     LAAADDHNYLLCYSTDGSYRYLSYRTRNSYLLMARDYLGLNLGRYGTKSKTEGISHRF
                     PARICNGFWCDDVYITEQVESEWEVRGYLPGIMNVVGDAYPYDRKVLEDFDGSGDSFL
                     YLRFYGHSSSPKGQMVNLTAWEV"
     gene            complement(14378..14803)
                     /locus_tag="ACFM75_000216"
     CDS             complement(14378..14803)
                     /locus_tag="ACFM75_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254824.1"
                     /translation="MSKFVMVVQAVYEGLERHEMVFLRDELERRIGALKDGAMPSETR
                     EWDKPAPAVLSVAGADASGFGEIGGGAPRYDWAALCARAPFQMFVAEVYPSFPDARSF
                     VMGRVCSPEDEADVAAAYCAWFEDKKLWGGDDPLGGVNV"
     gene            complement(14815..15222)
                     /locus_tag="ACFM75_000217"
     CDS             complement(14815..15222)
                     /locus_tag="ACFM75_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254825.1"
                     /translation="MANTLYDTSRQMFLEGQLNWKDDDVKVLLVNTTSYTVNVATHKF
                     LSDISSSARVTQPALLTAKTTTGGAADANDVTFTSVSGETVGAIVGFKDTGTESTSPL
                     LFFIDTATGLPLTPNGGDVIVTWDNGINKIFRL"
     gene            complement(15325..24609)
                     /locus_tag="ACFM75_000218"
     CDS             complement(15325..24609)
                     /locus_tag="ACFM75_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFC6254826.1"
                     /translation="MSDLVRYDPLEHGQLVGGLKKYRGFTQKDAWAAADDTALTRGFK
                     NSMRSARMAWNDLTGDKEELGQLKAEDMDYRKIQEGRKSQARRELGEAWEKGEGVGGG
                     LSNVWEELKKDWREKGLDGVLEDVGEMGGATLEQAPNALVPLATTTVGGILGTLAGGN
                     TAVGAYAGATLGNTLMEYGEQLDRAAEAAGVDPTDKDAMMAFIDRGAPGALKNAAVKG
                     AVVGAVDMATMKLGGSILNMGKKAAEKAALKKMGVAAADKAAVAAAKGTPEFAALAKE
                     SAKGGLGGAARHAAAFALEPASEFAGEYLGTGLANGEWDEKGAALEAFSSLGHSAVEF
                     VGTKAYAAISDPLRLSAEQKADALADAKGNIEGNRKAGKLTPEEAQALGAAVEAALDG
                     GVEQGGAGFDTAHHDQLYLTLQQFVDRTKQKEAEQFFNSPADGNTPHAEELAREMEKQ
                     PDVSGIPSEDEAEFLNTGVMSDGLARQYADMAAKYRAKPSEAMGIDPDDGAISAAAAL
                     AVDSGAAVPSVPSDDVETPTVADDVLSGRSADADRGGVSSAYGNVRSGGAASVASGGA
                     GRSAAAASGGIARVAPLPAGQYFDGLDTRGRKALAKEAGFDIKGVADFGQIAEPVRQK
                     IEEAYHARIEADYQAASEAKQGYLPPPVRMADAVPVPKKGFSVPADALNKESRRRFDA
                     LPETVRRHAQTVADYTADGIMRQEAGMADMRGHYPAGLAESVRVVRAYRAEHPESADV
                     LDRLNRAVYGYRRNNGWRVPLLSREGERLQGVRTALPDDGASEAVVGGGRGLPRALPT
                     EDKGLAQDVRQDVRQGLTQGGRGLTPDTGADANAAALQGLPESAAVASGNAPTHRQNL
                     QVRAHAEGAAPGLSASENLAETDGGKRAPVAGKRPDTVLPVLNPQVAESAVKVSPKKR
                     MADAAADFTRRLAADRRKLEKAGVPLGDGEYRFEHTNRQHIDALAGVLDRLGKGGMLE
                     EFADMAGSSHSDGLVFDSRRYLKGKEAETLQAGGLLEAVPSKSGWDYRLTQEGRALAK
                     VMARSRDAAASAGKSAGKAQSARAKDTPVAGKAAVAKNAANEKPSSDKAAKPETLVKT
                     ALDNPQEARRKARVLQGEPVYTVKERTAPGENKALREWAVALFDKAGGKAVNPEAGEI
                     LLNERSVRDSIAHGMNPFKAEAFAAIPDVISQGVVIHEGVNPENGTRYVYISAPVEIE
                     GKDDVVTLLARNTGNGSQMYLHSVATKESILNASDTETAEISRETGKVNSGYIASVLK
                     KLLKYKTQGGKTEDTPSEGLRFSRSEQTKSAFENRIDALFGGAKANLKGVRVLDKSDL
                     LDMLGYGGMPVELNESKVVLNRKNHPEMTAQQWKKVPKWLDNPVAVLKSKTHDKRLVF
                     VPDELIDGAPVYLVVEPNSRGLDIHVLVNGYAKDGNPQQTFRDIWRDLANGNTEFVDS
                     KKARELLGRSGLQLPRLPSLNTSRKKILTEKNLQGYRKSEKTDTGQQHAESIKKRLAE
                     SIGRLAEQVDVAAVSETAPDKAQMLLSERVEGWFDGRTGKITLVAENLTPERAVWVAW
                     HELGHRGFAADGFAKYRAELERADGNSLIRRIADAVQEEREGTGDAAASVRSAAVEEA
                     VVELYAAQRTGDWSGIENRYGVKVGNGLKRGIAGVLARIGAVLRRVLQRLAGKADGAM
                     SDADVFAMLADLHGNAEGARDASSDGVKPSRSAMKDTDANIRRGRAAMNRALVEKADV
                     RRAMYRNDIGWIDFVWGGTGRVLPNGKTKGAMGLAHVIESRMRKDGMGRDEVVGMLTN
                     DIVEAIARGKVYKHVVNGNSERLQLRHNGYEANLIKNAGNNAWLITGYELYQNGADGV
                     GFDTSAATHTTPTPARRDVGASDTPNVRAKDESVNTADGTRNSRAAERSKSESLEKLR
                     RAETIRISGREIEAGDDLRQYRRRAMEYGKSLRGSYVNKDTGREISLGRAGITEVLHH
                     DVSNPEHLQSIAAIPQIIEKSIYIDTLPNEDKAKNPDIQEYEYYVAGLNIGGTDYTVK
                     AAIAVATTGDKYYDHKLTRIEKGDLLEMTSRLSSAEISNQSPLSDIDDKRLLQILQDK
                     DAGKGGIADFDTEAVRFSRAANIGAAIGHITGKKSDLRNALKDRWDASKGIQLQFLGR
                     RQIEDIYGGDLDGLKEYGRLSELFGADANKAVTEADKVVREWGRLKKADAKALADLMH
                     DATLAKVDADPLMRGDAQKRLDGIRTALDIADGKIVKARAVIASADARIARADAAYNK
                     ASEAAKKAQSALLAAEEKAGREILADEADMRLRRLFYADSEAKRALRHAEADVMAESR
                     AKTDAVQMLKQARADVRRLEKDEVGAQKALEGLALLNRRFAKLPDAAQRVYRKARDDY
                     RAHFGQVRDALAERLARSGQDAEIVRRLKERFDNELGGVYFPLARFGDYLVVVKDADG
                     NSVNVSRAETLSEAEKLRDALKADFGAGFKVSPVMKSRDYIQSRDAVSGGFMKELGEA
                     VGMLDLDPAQQAQLNDTLTQLYLNSLPDTSWAKHGIHRKGVPGFGDDARRAYAQNMGS
                     GANYLAKLRYADRMAEQLDVMQDFVDGRKYVEGFNQRQLQRVADEMRKRHEAVMNPNP
                     SKLAQALTGFGFLWMMGMSPALAIVNLSQTAMVAYPVMAAKWGYADAARELLRASKQI
                     GLKVGEKFNTIEDSLNEDEKAAFQKAVDYGVIDLSQAHDLAGVANGDPGLAGSAWQKV
                     MDKASWLFHHTEKFNRQVTFVAAYRLAKRAGADSDTAFEQAKKATYDGHFDYAAQNRP
                     RFMMGNVAKVVFLFKQYSQNILYALGRNAYLAFKGDKEARKTLAGLLVSHAMASGILG
                     LPFVSTLLAVASMLGSDDDDPWDAEAALRNMLADTFGDKAGEVMAKGFSRLTPLDVSG
                     RLGLNQLIFPDIQDGLAGKKWAESLVVGSTGAVVGAGIGAADGVQKILDGRYMEGLES
                     MLPVAIRNPLKAVRYATDGQVDKSGITVKDDFNLFELAGQAAGFRSSDLALKQEGKSA
                     VYRRDRALSAARAKILSAMAKAVVEQDAAALRELRGVVAQWNRKHPERAIKSENIMSS
                     VRNRQRRVAGAKDGIYLSDKHSDARTDGGFAFGH"
     gene            complement(24606..25802)
                     /locus_tag="ACFM75_000219"
     CDS             complement(24606..25802)
                     /locus_tag="ACFM75_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254827.1"
                     /translation="MGLFEPTAGDFSEMKEKEKKEKTREEMEAQEREAALVRRADVVR
                     QTVANYEDGLARYRNVMDLSRNNIEDGARRLRQAGAFERDADAGLGFSGGDKVLSPDA
                     RAEADFARRDTRSTDAGGQTLPSLGFDGNVYRGGKLVRDFDAQRPLVSAKPDALSPEE
                     QELYKRATTPHAGALNGQLTAAQLNAARGIVAEHNKNAADGELGRERLAAAAAENAAN
                     REAMLQKGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LENRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFEQKEEKGFDPNVFKTISYEIT
                     DPNTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAALGFKPNGEKTADGKIIYENDRG
                     EKRVEQ"
     gene            complement(26068..27264)
                     /locus_tag="ACFM75_000220"
     CDS             complement(26068..27264)
                     /locus_tag="ACFM75_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC6254828.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWIYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVAR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRSLARTYLREVLKVDNDVAEKLLAHSLGTRTQTAYNRSELWEERKDALERWGNDVLR
                     LAKNGK"
     gene            complement(27456..27545)
                     /locus_tag="ACFM75_000221"
     tRNA            complement(27456..27545)
                     /locus_tag="ACFM75_000221"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27509..27511),aa:Ser,seq:cga)
     gene            complement(27623..27713)
                     /locus_tag="ACFM75_000222"
     tRNA            complement(27623..27713)
                     /locus_tag="ACFM75_000222"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27677..27679),aa:Ser,seq:tga)
     gene            complement(27796..28575)
                     /gene="yaaA"
                     /locus_tag="ACFM75_000223"
     CDS             complement(27796..28575)
                     /gene="yaaA"
                     /locus_tag="ACFM75_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC6254829.1"
                     /translation="MFFVLSPAKNLNEKDPASVSEFTQPDLLAESEILMQQLRELALQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRTRLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(28839..30026)
                     /gene="dapC"
                     /locus_tag="ACFM75_000224"
     CDS             complement(28839..30026)
                     /gene="dapC"
                     /locus_tag="ACFM75_000224"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC6254830.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEIFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            complement(30103..30555)
                     /gene="dut"
                     /locus_tag="ACFM75_000225"
     CDS             complement(30103..30555)
                     /gene="dut"
                     /locus_tag="ACFM75_000225"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC6254831.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLKPG
                     ETFLVPTGLVIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPVVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            30697..>31108
                     /locus_tag="ACFM75_000226"
     CDS             30697..>31108
                     /locus_tag="ACFM75_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFC6254832.1"
                     /translation="MMHTPSPHNEFIRAIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALAPHLKGIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNM"
BASE COUNT         7472 a        10262 c         6750 g         6624 t
ORIGIN      
        1 catataaatt accataaaac tgtgatcaaa gcatctgccg attgcggttt attgcagggc
       61 gttacggaaa ataagcataa tccaatgatt cttttatggt aattgtgcag attcgcgcca
      121 aaaagaaccg ccctgaatca gggcggtttt gttttctggc ggaaacggtg ggattcgact
      181 aaattttatt ttattgattt aaatatattt attctttgtg aaaatttaat ttaccataaa
      241 aacagccata tacaaaaatc ttggagtaac tattgcatta cactattaga agaatgccgt
      301 ctgaagtgtt ttcagacggc atttcccaag ctgccggaaa aaagctaaaa tgcccgcaaa
      361 cagacaagga gcagcgatgc aaaaccaaaa tacacgcccc gtcaaaatcg agcttaaagg
      421 cgaagcaggc aaacgcgtac tgcttgccgc cgcccgccgc attgccaaaa cccatcaaaa
      481 agccgtcaag gcacttgccg acaaatgata gacggcgaac tggtcgcgct tatccatcaa
      541 accgtattgg cggatgaagc gggtttgaaa gggcgggcgg atatggcgcg gctcgacggc
      601 gcattgtcgc ggattgccaa ctggcggcag tatgaaaacc ttgaggacat ctacgaaatc
      661 gccgcccttt atgcacaagc catagccaaa gcccacgcct ttcccgacgg caacaagcgc
      721 accgcgcttt taacgatgtt gacctatctg gatttgcagg gcatcagcat tgccgccgac
      781 caagggcttg acgacttgat tgtcagtttg gcggcggggg aaaccgactt caaacagctc
      841 gccgaaaccc tgcgccgact ggataaggaa taggcatatc cgacaacaat gccgtctgaa
      901 attcagacgg catttttatt gaaaggcttt tcttcaaccg ctttacacaa aggcggtttt
      961 tttatgccgt ctgaaagccc ttcagacggc attggtttac acggcaggag ttcccccgcc
     1021 tttaagcagg agaggatgtc aaggatgcct gatttttaaa tcaccccttg aaagaacggg
     1081 cgcacggcat taatatacag atatcgacaa gcaaggttaa aaccattaag gaaatacgat
     1141 gaaatacaaa aatgagggtt ttaatggctg gtaaggtgcg gaaacaaata aaacaaggag
     1201 gttggttgtc agtaattgcc ctaacctcct tgtttgtatc tgtattcact ttattttaca
     1261 tattcaggca cagcgtacag tttaacctat gagggacggc cgaatggctg atgttttggc
     1321 aactgaccgt tgtttcagta accgccgtca ttgcactggg gacaatattc atcaataaga
     1381 aaacttcaaa gcaaaaggcg acattagatg ttattttgaa tgattaccaa gatgcacaat
     1441 ttgtagaagc cgacaatcat atttcgcctt atattcgcgg cacggcagtt gacgacaaca
     1501 acgcgcggat cgacctgtat gaaatttatc aaaataaggg cggacaatgg gaaaaagaga
     1561 gagggcattt acttaccgta atcaatcggc acgagtttta tgcgtgcgca atcaactcgg
     1621 gagtattgga tgaggatttg tttaaacggc tgcattgcac caacttcata aaattgtgga
     1681 atgcagtttc gcctcttgtt atgaaaatac gcgaagaaga acgcaaagac acaatattta
     1741 gagagttgga aattttggtt gcattatgga aagcaaaccc cctaaaggca tctgatttgt
     1801 gaataaacag tcaaaactgt tctgaaatat gggcagccac gcaatcgccg aaatacgcca
     1861 aagcagcctt ataaagtgat tttttgaaca taattttctc cttgcggagc attttccaat
     1921 caaacagttt tagtttactt ggttttgtat ccctaaacaa ccgaaatccg acatcaagca
     1981 attagaaagc ttttgcatct tgaaaatgga taacaaaata ttgcctgaag gcgcaaatac
     2041 agtacaaaag ccgtccgaaa cagttcggac ggcttttggc gtattctgca caagtatttg
     2101 aacaactgaa attttatggt aatatgtatc tactttgtag atacaataaa ggtgaagatt
     2161 atgttgcgtg tccaaaaatg ggggaactcg gcggccgtcc gactgcctgc cgacatgctg
     2221 aaacaattgg attttaaaat cggcgacgct ttggtagcgg aagtacataa cggcgaactc
     2281 cgtgtgcgtg ctgcccgacg tttccgcttg gcagacttgc ttgccgaaat ggaagaaacc
     2341 ccgccgcgcg tagaaggctg ggaaatcttg gatgatgccg gcaacgaggt cgtctgaaat
     2401 gtatattccc gacaaaggcg atattttcca tttgaatttc gacccttcca gcggcaagga
     2461 aatcaagggc gggcggtttg cgctggctct gtctccaaaa gcattcaacc gcgcaacggg
     2521 attggttttt gcctgcccca tttcacaggg gaatgcagcg gctgcacgaa gcagcggcat
     2581 gatttcaacc ttactcggtg caggaacgga aacgcagggc aatgtccact gccaccagct
     2641 caaatctctg gactggcaaa tccgcaaggc ttcttttaaa gaaactgtac ccgattatgt
     2701 attggacgat gtgctggcgc gcatcggcgc cgtcttattc gattaaatgc tgaaaccgcc
     2761 cgaacctgta atcttttctt acaggttcgg gcggttgctt attcggcacg ctgactgctt
     2821 actgcatgac catatgcctg ccattccctt aaatcggccg catgtccgtc ggctgtttct
     2881 gccagtcctg catatttttc agcgcactgt ccgaataata gccagccttg ggaatctgac
     2941 gcgccatcag atgcggtggc ggcggtagtg gttgcgggca ggtttctgct gcgattttgg
     3001 gcggcggcgt agtggcgcag gcggttaagc tcaagacgca tagcggcaac agttttttca
     3061 gcattttgtt tttcctcttc taatttggtt tgtcgttcgg caaacgcggc ggcggctttc
     3121 tgctcggcag aacgcgcctg ctttgcctgc tcgatatagc cgtctttcag acgattcgag
     3181 atttcggcag cggcagcctc ccgtcccatg cggtattttt cggcacggtc gaaacgccat
     3241 gcggtaaaaa tcaggacgat cagcagcaat acgcctaccg gcttccagta tttcaataaa
     3301 atatccattt cagacgacct caaggatgca gcccgggcag atacacggtt ttgccgtttt
     3361 tcttggttgc ggtcagtatc tggttgcgtt gcgggctgtt gcgtcggaaa ccgatgtgta
     3421 cccatgcgcc gtccccgcgc tcaggaaatt cgagtatcaa ctggtcgaat ttcagcgcgc
     3481 cttcatcacg cattttgatt aacagtttgg caaatgccag cgaggtcatg cccgatgcgt
     3541 cgcaatcggc ggcaagtccg tgtcggtgcg ccgatgtggg actgccaccg accaatttgt
     3601 ttacccgctc gctgcgcaag cagcttgtta cgataatcgg gcgaccgacg tattcgcgga
     3661 ttttttcaag ctgctgtgcg gtgtaataaa tgttgtccat ttcggcgggc gacggttcgt
     3721 tcggtacacc tgcgcgacgc gcggtttcac tgcgtgtcaa ctcgcgcaga ctgaaatttt
     3781 tggtaacttg catttcggtt ctccatgttc ttttcaaata aaaaaccgcc taaacgacag
     3841 gcatccccaa cagatttcac gcggcatagc gtttcaacat ttcaaaatac atccattaag
     3901 aactgctata atagccgcct gtcatcgtca ggagcggcta atgcctttaa aattccaacc
     3961 acaggaacgt tcggtcgtca tgtgcgactt tcgcggctat gaagaaccgg aaatggtcaa
     4021 gaaacgccct gttgtcgtca tagcgcgaaa caggcacaac ggcaaactgg taacggtcgt
     4081 acccttaagc agcacagaac ctgtcccttt ggcggactac caccacaaaa tgagtggaaa
     4141 ccccttaccg gacaagccgc acatccaatg ttgggcaaaa tgcgatatga cggcaacagt
     4201 cggattggca cggttagacc gatacaaacc caaagggcgc gaccgctgca ttccaataat
     4261 cagtgaagag gattttcagg cgattaaaac agccgttgcc aaggcattca aactgtacta
     4321 gaataaatct gttcccttaa aggggcttgc aagactgttc tgaaatatgg gcagccgcgc
     4381 acgggcgaca ggcgatgaca agccgtccgt gcgtgtgatg gggcgcggaa tgcgcccctt
     4441 gtcatatctg caaacgccta caaatcccaa ccagcctttc aagcaaagcc gttttggata
     4501 aacccgcctt tgccgcctgc tgttttcaga cggcatagcg tttcaacatt tcccgataat
     4561 tcgccaattc ccgttcggca aattcaaagg ccttcacatc gtcgttatct gatgccttgc
     4621 ggcgttcgtt cagccagtag gcgatttgtt tttcacaaaa ttctttggcg gtcatcgtca
     4681 aatccaaaaa tgcctgttca agtgcagcgt atcctcacgc gtgatacggc gcaaatcact
     4741 gtcggtcggc aggccgaaat acagctcgaa acgcctcctt gccgattccg aacgctgcgg
     4801 gtcgaaagtc tccgtatccg ggatactgaa agcctccgcc aaagcccaat aaaccaaatt
     4861 ccgatgatgt atagaattga tttccggcgc gtccccgtcc tcctccatat cccgcggcag
     4921 gcaatacccc ccgacaaaca acctgccgtt acggtccggc gcaggtgcaa gcgtcagctt
     4981 ctcatcaccc tgcaccgcat aaaccggcag ccccgttgcc gccccgtcgc gccaacccgg
     5041 ggcgcaaaca tccgccgtct ccggcgaaac caacttcagg cacaccgact tgccgtcaaa
     5101 cacaaaacgg atactgtcga tttcatacac ataacccgcc gcaaactccc ctgtcgcata
     5161 agcataaagc ggccgccctt tttccacatc cgttacaaac gcatcgtccg aatgcagcag
     5221 ccggccgcgc aggcacgcct catgaaccgc atcgttcagc catcccgaca cctcctcgtc
     5281 gcgccaaaaa tacggctcgg ccttatcatt ggcaagcacg cggaaacgcg caatcaaagc
     5341 cttcaaattc attgcacgat accaaactga tgaaccatct gaaccacagc ctcacgcaaa
     5401 ttctccgccg atttgcgctt atccaaactc tgaccgtaat tcgccttcgc atacagttca
     5461 agctcgtcct tgcccatccg gttgatttgc aggatcacat cctgcaccgc atcaaacgcc
     5521 gcctcttccc gttcgacttc caaagcaccc gccttttcca aagcctccgt atcatccgcc
     5581 ttgatcacac gtttgcccgg ggcggcgaca aacacatcac cgtggcgcaa caactttgcc
     5641 gcagcctcgg ccgacacctc gcgcacctga ccgtcttcaa aaaccaagcc cgtatgatag
     5701 ataccgtcgc gccacacggc acgcccgccg atatacttga taaaaaccct atccattcaa
     5761 cctcccgccg ccgttcggac ggcattgtca aatacagaaa cgccgcaaag gcaaatcccc
     5821 aagcggcgtt tcattccact tacaactgac cgaacttctc accgtaaatc agaatatcga
     5881 tatcggcagc cgccttactg tccgcccctt taagcgtaac cgtcagcaaa gcctgcttgg
     5941 gcagcgtaac cagtttggca gactggcagc ggatacgcgc ggcagacgca aagtccgcgg
     6001 ccgaagcaaa ataagccgca tcctgaggca cgttcgcatc atccgcacca tccgcataca
     6061 caaagccgac atcggcagtg acaccggaag ccattcccgt cttcacaata atttgcgcgt
     6121 catccaaacg gaaaccctgt tccaagacac ccaacaccaa cacatcgtcc gccttaggcg
     6181 gcccgtaggc atccgccccc aagatggtac cgtcctcctt cgcaacgata cggtaatgct
     6241 ccaccgccat attgccgtaa ggcacaccgc cgaaacggtt gccgccattg tttctcacat
     6301 gaatcttagc cataaaaaaa cccctcaact caacaaatag aaaaagccgc cctttcaagc
     6361 ggcattcaaa tcagcggata ccgccctgca aaggaaccac cgtatccaca accgttgcac
     6421 cgtagtcggt aatctccttc tcaccctcgc cgacatccac ggcaaagcgc gttttcgcca
     6481 caccgcgtat cgtaccgatg agcgtttcca cacggttgcc gtggtcaagc tccttttcag
     6541 accaaaagaa aggcataccg ccgtgtttgc cggtattcgc aaacgcctcc aacaccgcct
     6601 gaccgcccaa aataaccgaa cggtcgaccg caaacttgtc cgcaaacgaa gccgggattt
     6661 tcaagcccga ttccgcctca ctgaacttat cggcgcaata cttcatctca tcgcccgcat
     6721 agaaacggat aggacgcggc atttttacca agataaagcc gttccacaaa cccgcctcgc
     6781 ccaaaaacag cggattttga tttgcctggg aagcacgcgc aattgccgaa gcctgaagct
     6841 ggcggaattt cggatcggcg gcaaaacggt tgtactgtgc cggagaaagc agccacacac
     6901 gcaaaggcga atcactcgcc gccttatcgc cctcaaactt cacaatcggc ggcggcagcg
     6961 gaatctggtc aagcacggta cgcatactgt ccaccgtatc catcgtaaac aaatcggtag
     7021 aagcaatctt caactcgccg cctttcgccg caacgcccgt taccgcaccg cccgaagcca
     7081 caaaatggcg gtttttagac ggagctttga ccggattgac cacataatca ttgaatttcg
     7141 ggtcgcccgc caaaggcacg ccccattcga tattgttatg gaaaccgcgc gcgcccgcca
     7201 tatgcaccaa caacgtctgg tcggcataac ggtccatcag gctttgcgcc accggacgca
     7261 tcagcgcacg ataatcggca gggctgcgct gattcgtcat cgtgccgccg ccgtcaacag
     7321 ggaaacgcgc ctgattcaca cgcaaacccg cctccgacag gctcatcccc acgcccttgc
     7381 cttccgccgt gttatcaccc ataatcggca gggcagaaac agggttgacc aaattaaaac
     7441 ggatttcatc gcccataccg cgcgtcaaat cctgacagcg cacaatcggc atatgctggg
     7501 tcgtctgttt gcgcaaagtc gcctccgcac ccgccgtacc ggcaggcatc ttacccgcca
     7561 aacggttcag cgtactgtta cgctgcatgt gcatcgcaaa cagccccgcc gcctgcttca
     7621 tcatcgcctg cgggtcgccg taggccatat tcgtcttttg tgtcataaaa agtctccctc
     7681 aaaaactcaa gaatttaaaa acttctcaac ctgctcttcc gtcatagaag ccagtttttc
     7741 cgccagcgca ttaccgctca aatgatccag cgggctgtcc gaagccgtat ggtcgcgccc
     7801 cgccggaata tccgagagcg tattgggcgt attcttcctg accggcggct tttcatcagg
     7861 cttttcatca ggcttttcat caggcttttc cggttcggcg gcggattttg ccgccttaaa
     7921 cagatcaaac gccccgatga cctcggcggc agtcccgtcc ttaagcgcat catgaaacgc
     7981 cttacggaca agcggattct gccccgccag ccactcctcc agctcacggc tttccacaat
     8041 cgaatccgca tcagggtgcg ccgtataaat ttcgcggtaa tgcgcctttg ccgcctcctg
     8101 ctgcttcagg ttcgcctcat aacccgccag cttctcctga atcagctttt ccacgccctt
     8161 tttcatatcc tcgtcggaaa aatccccgaa caacgaatta ccggaatcgt cagccgtttt
     8221 cacttcaggt ttttccgccc ccttctcaag ctgggcaata cgcttcctca aggcttccgc
     8281 ttcggctttc gccgactttt ccgcctcacg cgcctctacc agcttttcat aagggatcgt
     8341 gtgtttgccg tccttggcac gaatgacctt gtccgcatca tcgtccggca caccgccgtc
     8401 cgacccgtct tcccgggcat cggcatcact ttccgaagtt tcaggtttcc cgccgtcggg
     8461 cgcatcggcg gcatcttccg catcaggtgc tttttccccg tccgcatccg tatcgcccgc
     8521 agattcagcc ccatcgtccg cctccaaaac cgcaagctgt tcgggggtca gaatcaaacc
     8581 caaatcctcg taatcgatct cgtcttccat ttcctctcca tgccacatat cgccgtagcc
     8641 gcaaaattaa aaggatacgg tatggtaaaa acaccgcaaa aaaccttcaa acgactaaga
     8701 atcaagcatt aacgaaacaa accgcctatc ggcagacacc gtttgcgccg ccgttcccgc
     8761 cgccaagccc cgcaacaccc ccgcgtgcac ttccaccaca ccgccgtccg gcagccccac
     8821 cgccgcacca ttgtccgcca accacaaaca caccgccgcg ccattggaaa actgccccgc
     8881 ctcatccgca tccaaacgca cagaactcat cgccacaggc ggcttgaccg ccaagcgcac
     8941 catttccaaa gcctccacat cgacaccgcg caaaaacacc acaccgtcaa cctgccccac
     9001 aaaaataccg ccctctaccg gctcgacaaa cgtaatcctt tgcggcatct gcatatagcc
     9061 gaaccgaccg tcatgcagat ggaaggcaag cggctcggaa aagcgcaaca cattcgccct
     9121 cgccgtcagc agccgcccgc gccacacaca cataaacttc cccgtcggca taggcgacaa
     9181 aaaacgcatt tgcgccgtct tcccctgttt aggaagcagc ggcagcacaa tcgtccccga
     9241 caccggataa tccccgatac acgccaattc cccaccgtcc gcagccgtcg cataaagcct
     9301 taccccgtca accgtcctat ccgtacaaaa cggcagcgcg acttccacgc ccccgccgtc
     9361 cgcaaccgca accgaagcca tagccgacaa accgccttcc ttgccaccgc gcagccaggc
     9421 cagcgcaaac ccataatgcc ccgccgccaa agacccttcg gacggcatca cgagcggcgc
     9481 gggcggcgta tcgataccca acctttgggc agagagcccg tcataaacaa aaatccccct
     9541 gtccgttgcc gcaaacaccg catgattgac cactgcatac cacacgcgcc ccgcccccgc
     9601 atcgcgcacc aggacggaca cagaaaaatc ccccgtatcc aatttgcaca aatgaccctc
     9661 atgcacacaa aacacatccc tatgcaaagg gctctgccac acatcccgca gcttcaagcc
     9721 gctgattttc cgcgccgcac cgcgcataga aaacgccccg tccgcatgaa tgtccacatt
     9781 gaccgcatcc cgcacgaaac gcccgggcgc atcggggccg cgcaccatat catcgtcccg
     9841 cgccacattg ttcacacccg caacaggcgt aatccctacc tctgccatac actcgcacct
     9901 ttcctgaact ggtccgcata cccgtcggga cggataaacc gaaccttatt tccaatatcg
     9961 catctgccga acggcgcaaa gccgaacggg ggtacgccga caaactgcac cggccgggcg
    10021 tcttccccct tgcgccccac acggttgtcc ggcggcatac ccgaagcacg gcgcaccgtc
    10081 atcctgtcgt caaaccgccg cgcctcccct tccgaagcaa acatatccat accttccgcc
    10141 cgcaccgtcc tgttccggta cgacaccgac ccaaagccgc cgaagcgcag caaatccccg
    10201 ccgccgattg ccgtcggtat cctttccccg acgcgcaagc cctgccacat aaacggcgta
    10261 tcgttccaac ggcttttgcc cgcctccagc atatccgccc ctgccgggcg cacatcctga
    10321 ttgcggttgc gtaccgtctg atccccgaaa cccgcaaacc gcacaccggc ggcggcgata
    10381 cgccaatcaa acacatccag aaatcccgcc ttaagcacgc cgaaagccat ataatcggca
    10441 ttcacgtccc cttgcgaaaa cagttccaca tactgcgtat aaggataaaa agtattccag
    10501 cccgcacgga aatgaaatat ccccttcgcc cgcacagact gcctggcatt ttcaatcagg
    10561 cagtatcccg acaccttgcc cgcccgatat tgcggatagg ccgcatactg ttcgacccgc
    10621 ccgctgttga acacaatata cggatagccc gtccggatac cctccgaagg cgcgtggtaa
    10681 ccgcctccgc cgttgttttc cgcagccttg ggcggcggcg aatacatcac ccaaaccgta
    10741 tggggcgtaa cgcgcggcct gcccaacgca aaatccatcc gccatccttt cgcaacgatg
    10801 ggtctgtgcc tgtgcgacac ggcgcaccgt cccgccgtcc cgacaggaat gccctggcga
    10861 accaggatac cgttattgaa aaaccgcgtc ccgatactga aaagcgacgg cgcaaacccg
    10921 cccgcgtccc tgaccgacat atcggtcaga cccgtacccc cgaacagcac actgtcccag
    10981 ccattgcccg acccggacga agcaccgtcc tccgaagacc acgcatccag caagatcagc
    11041 tttgttccga aaggcggcgt actgtccgtc cacacatcgt gtccgtccgg cacgcgcatc
    11101 gtaccgatac cgcccatacc gatgaagcgc acccgccgcc cgatccacgt cagccgtccc
    11161 aacgtatggc ggttaaacgt ttccacccca agcgtcctga cccagtactg caccgccgcg
    11221 ccgtccgaaa acgcaaaacc cccgaagccg tgcagataca cttccgcatt gcgctttttc
    11281 aaataagcca gcccgaaatc ccgcccgccg tcgtccggcg gccaagaagg cgcaatcatg
    11341 ttccgccgta tcctgaaaac ccccgtcccg aaggccgccg aatccgtacc gcgcaccggc
    11401 acataccgct tcagcagcgc caccgacggc atacgcatat cgggcgggcg tatcataaac
    11461 gacagcgaac catcgtcctg aaagatatgc cgtaccgcgt atgccgcaaa cgccgtgccg
    11521 tacgcctccc cctcaatccc gcgaaaacgg cgcaaaaaac ggcggttttt ccacacatac
    11581 gccccgcgcg tatgcaccgc catatccgtc cccggcggca gcagcacctg cccgttcgtc
    11641 cacaccgccg cacccgtcgg gattctcgcc ccgtcccatc cggacggcgc ggcatagcgc
    11701 accctgttcg tcacagacgg cttccccggc tttggcggcg gcagcgtggc cggcacaaca
    11761 cgctcccccg caagcagcag caccgtataa ccccagcgca ccgccgcaaa acggctcggg
    11821 cgcacgacgc ggtcgcggtt gtacggatag gccgcaccga acaccatccc gtctatctgc
    11881 tcctgcacct gatccgaagg ccgcacatac tgcctgacat tgcgcaggat atgcgcccaa
    11941 ttgttgaccc aaggcgggat gctccaagcc gacacgcgca acaccgactg ccgcaccgaa
    12001 gatacgccga acgacacccg caccctcatc cccgcatcaa cgcgcaccgc ctgcccgggc
    12061 ggcacgatgc gcgtcagata agccgccgaa tcccagccca acggccgtat ttccggcggg
    12121 aagcccaccg tatggttgcg cgaaaactgc cgccgcctga aactgttggc aggcagcacc
    12181 acatactgcc tgccgaaact cacgcgcacc gtccgccgct gttcgaaaaa gccgccgatg
    12241 accccctgcc cgatttcaat atgccgcaaa aaatgcgaaa accacgcttg cccgaacaca
    12301 agcgaatcaa aatccgcccc gcgcccgacc ttctgcaaag gatcgaacac acgcgccacg
    12361 ccccacgcaa accgattttc aaccgtcgcc aaaacccggt aggtacggtt gaatacaatg
    12421 gggaacggaa atgccaaatc atcgccgtgc ggccgctgcc tcaggcgttg ccccgcattg
    12481 cgtaccgata ccgcaccccc cgccgccgac acatccccga gcgaaacccg gcgcacatcc
    12541 tgaatcgtcg aatacacccg aggcgtaccc gcatccgaac cgtacagacc cgggaaaaac
    12601 aaataagaca cttccgactt gaagccgaaa ggcagttccg ttttcgcatt tttggcggat
    12661 agcggcagcg caaacaccaa aggcagcacc ctttccacca ccaaatgccg tctgaaccta
    12721 cccaacgact tcgccgcacc ctcgccctga taaaagctgt cccaaccctc ggaaacaaca
    12781 agaaagtccc ggttgtatat cttcgacctg tcgccgaaga cagacggcgc aaaccccacc
    12841 gggtaaaacg gaagattgga aataaacacc gccgccccga accgttccac accgtccgaa
    12901 gcctgcgcgt caaaaacagg cgtttcccaa ttccacaccg aagcacgtcc gaaagcagac
    12961 gtatcgccga gtttgaacca cacaaaaaaa cggccgccac cgtcgccaaa cggaagcggc
    13021 ccgacaccgt cgccatcccg cctcacatat ccgaagacga gcggcagccc atgaggatta
    13081 attctatcca taactatttt tcaggcttca cattgtccgc cgacacaggc tcatactttc
    13141 ccgtatggtc gcgtgccagc accaaaaacc gcttttccgt gtccaattgc gaaaaccggt
    13201 actccccgtt atcgtccgcc cacacatcat cgatacacac catatccggc agcgcgaaca
    13261 aatacacccg cctgctcgca ggcgcaccct ccaccgtgac cacgcccctg cccgcccccg
    13321 caatcaagcc gtcgccaaac tgataatggt tggcggcaag cagccccctg ccgcccatcc
    13381 cgacaaacgg cacatcctta cgttcgtgcc gtttcaatat caaaacaccg cccctgctga
    13441 agcccaacag cccgacggca ggattgtcgg caggattgtc cgccatcaca cctcccaagc
    13501 cgtcaggttg accatctggc cttttggcga actactgtgc ccgtaaaacc gcaaatacag
    13561 aaagctgtcg cccgagccgt cgaaatcctc aagcaccttg cggtcataag ggtacgcatc
    13621 cccgacaacg ttcataatcc ccggcaggta gccgcgcacc tcccattcgc tttccacctg
    13681 ttcggtaata tacacatcgt cgcaccaaaa cccgttgcag atacgcgccg gaaagcggtg
    13741 gcttatccct tcggtcttgc ttttcgtgcc gtatctcccg aggttgagac ccaaataatc
    13801 ccgcgccata agaagataac tgtttcgggt acgataagag agatatcggt aactgccatc
    13861 cgtcgaatag cacaaaagat aattatggtc gtcggcggcg gcaagcgtcg gcacgcgccc
    13921 gaaatacagc gtcgtgttaa acgggccgtc actcgagtgt tccgttacgt gaaaaatcaa
    13981 gatgaagccc aaagaatgcc cgaccagcgt ccaatccctg gttttagtat ccccgtttgt
    14041 gttatcgtgc aagaaatacg cctcattgtt ttcataagcc gattcaagtt caaacgtgtc
    14101 atccgaagac ggattgcgta ccgccgacac cctcgcgccg tacttttccg actggctgat
    14161 acgcaaaccc catcccgtct gcttcttatc cgtcggcata aagatgcccg tactcccgac
    14221 cttgaacttc aattcccagc ccaaagacgc ggtatcgccg taaccttcca ccaggcaggc
    14281 attcaaaatc ttaaacgcat tatcggacgc acctaccaca ttttgcggcg cacccttgtc
    14341 cgaagagcgg aacacccgca ccggcacacg gcttttctta aacatttacc cctcccaacg
    14401 ggtcgtctcc gccccacagc ttcttatcct caaaccaagc acaatacgcc gccgccacat
    14461 ccgcctcatc ttccggcgaa caaacgcgcc ccatcacaaa agaacgcgcg tccggaaaag
    14521 atggatacac ctccgcgaca aacatctgaa acggcgcgcg cgcacacaaa gccgcccagt
    14581 cataacgcgg cgcacccccg ccgatttccc cgaaacccga agcatccgcc cccgcgaccg
    14641 acagcaccgc aggcgcaggc ttatcccatt ccctcgtttc agacggcata gcgccgtcct
    14701 tcaaagcccc gatgcgccgt tccagctcat cgcgcaaaaa caccatctcg tgccgttcca
    14761 aaccctcgta caccgcctgg acaaccataa cgaatttaga cataacgccc cccgtcacaa
    14821 acggaaaatc ttattgatgc cgttgtccca agtcacgatc acatcgccgc cgttcggcgt
    14881 cagcggcaga cccgtcgccg tatcgataaa gaacagcagc gggcttgtcg attccgtccc
    14941 cgtatcctta aacccgacaa tcgcccccac cgtctcgccc gaaaccgaag taaacgtcac
    15001 atcgttcgca tccgccgcgc cacccgtcgt cgtcttggca gtcagcagcg caggttgcgt
    15061 cacacgcgcc gaagacgaaa tatccgataa aaacttatgc gtcgccacat tgaccgtgta
    15121 agaagtcgta ttgaccagca acaccttcac atcatcgtct ttccagttca actgcccctc
    15181 caagaacatc tgtctcgaag tatcgtaaag cgtattcgcc ataactgctc caacaagtta
    15241 aaaacacacc cattaaaccg acagcatcag agacattcaa ccgcctaaaa aaaagcccga
    15301 accgttttca ggttcgggat tttctcaatg cccgaaggca aatccgccgt ccgtccgcgc
    15361 atccgagtgc ttgtccgaca aatagatgcc gtctttcgcc cccgcaacgc gccgttgccg
    15421 gtttctcaca cttgacataa tgttttccga cttaatggca cgctccggat gcttcctgtt
    15481 ccactgcgcc accacgccgc gcaactctcg caaagccgcc gcgtcctgtt ccaccaccgc
    15541 cttagccatc gccgacaaga ttttcgccct tgccgccgac aaagcacggt cgcgccgata
    15601 gacggcagac ttaccctcct gtttcaaagc caaatccgaa gaacggaaac ccgccgcctg
    15661 acccgccagc tcaaacagat taaaatcatc cttgaccgta atgcccgact tatccacctg
    15721 accatccgtc gcataacgca ccgccttcaa aggattacgg atggcaacag gcagcatcga
    15781 ctccaagccc tccatatacc ggccgtccaa aatcttctgc acaccgtccg ccgcaccgat
    15841 acccgcaccg accaccgcgc ccgtactgcc gaccaccaac gattccgccc acttcttacc
    15901 cgccaaacca tcttgaatat cagggaaaat caactggttc aaacccaaac gccccgacac
    15961 gtccagcggc gtcaggcggc tgaacccctt ggccatcacc tcacccgcct tatccccgaa
    16021 agtgtccgcc aacatattgc gcaacgccgc ttccgcatcc cacggatcat cgtcatcact
    16081 gcccaacatc gaagctaccg caagcaacgt cgacacaaac ggcaacccca agatgcccga
    16141 agccatcgca tgcgaaacca acagccccgc cagcgtctta cgcgcctcct tatcgccctt
    16201 aaacgcaaga taagcattgc gccccagcgc atacaggata ttctgcgaat actgcttaaa
    16261 cagaaagacc accttcgcca cattgcccat cataaagcgc ggacggtttt gcgccgcata
    16321 atcaaagtgc ccgtcatacg tcgccttttt cgcctgttca aaagccgtat cgctgtccgc
    16381 ccccgcccgc ttcgccaaac ggtaggccgc gacaaacgta acctgacggt taaacttctc
    16441 cgtatgatgg aacagccagc ttgccttatc cattaccttc tgccaagccg accccgccaa
    16501 tcccgggtcg ccgtttgcca cacccgccaa atcatgcgcc tgcgacaaat caatcacacc
    16561 ataatccacc gccttttgga acgccgcctt ctcatcctcg ttcagactat cctcaatcgt
    16621 attgaacttc tccccgacct tcagcccgat ctgttttgaa gcccgcagca attcccgcgc
    16681 cgcatcggca taaccccact tcgccgccat caccggataa gccaccatcg ccgtctgtga
    16741 caggttcaca atcgccaaag ctggcgacat ccccatcatc cacaaaaagc cgaagccagt
    16801 taaagcctgc gccagcttgg aaggattcgg attcatcacc gcctcgtggc gtttcctcat
    16861 ctcatccgcc acacgctgca actgacgctg gttgaaaccc tccacatatt tgcgcccgtc
    16921 cacaaaatcc tgcatcacat ccaactgttc cgccatacgg tccgcatagc gcaacttcgc
    16981 cagataattc gcaccgctgc ccatattctg cgcatatgcg cgtctcgcat catcaccgaa
    17041 gcccggcacg cccttgcggt gaataccatg tttcgcccaa gacgtatcag gcagggagtt
    17101 caaataaagc tgcgtcagcg tatcattcaa ttgagcctgt tgcgcagggt ccaaatccaa
    17161 cataccgacc gcctcgccca gctccttcat aaatccaccg cttaccgcat cacggctttg
    17221 gatataatcc cgagacttca tcacaggcga aaccttaaac cccgccccga aatcagcctt
    17281 cagcgcatcg cgcagctttt ccgcctcact caaagtttcc gcgcgggaca cattcacact
    17341 attcccgtcc gcatccttaa ccaccaccaa ataatcgccg aaacgcgcca aagggaaata
    17401 cacaccgccc aactcattat caaaacgctc cttcaggcgg cgcacaattt ccgcatcctg
    17461 ccccgaacgc gccaaccgtt cggcaagcgc atcgcgcacc tgcccgaaat gcgccctata
    17521 atcatcccgc gccttgcggt acaccctctg cgccgcatcc ggcagcttgg caaaacggcg
    17581 gttcaacaaa gcaagcccct ccaaagcctt ttgcgcccca acctcatcct tttccaaacg
    17641 ccgcacatcc gcgcgcgcct gcttcaacat ctgcaccgca tccgttttag cccggctttc
    17701 cgccatcaca tccgcctcag cgtgcctcaa cgcccgcttc gcctccgaat ccgcataaaa
    17761 cagacggcgc aggcgcatat ccgcctcatc cgccaaaatc tcacgtccgg ctttctcctc
    17821 agcggcaagc aaagccgact gagccttctt cgccgcctcc gaagccttat tgtaggcagc
    17881 atccgcacgg gcgatacgcg catcggcgga agcaatgacc gcccgcgcct tcacgatttt
    17941 accgtccgca atatccaaag ccgtccggat accgtccaaa cgcttctgag catccccacg
    18001 catcagcggg tcggcatcca ccttcgccaa cgtcgcatcg tgcatcagat ccgcaagcgc
    18061 tttcgcatcc gccttcttca acctgcccca ttccctgaca accttgtccg cctccgtaac
    18121 cgccttgttc gcatccgcgc cgaaaagttc cgacaaacgc ccgtattcct tcaggccgtc
    18181 caaatcgccg ccgtaaatgt cctcgatctg ccgcctgccc aaaaactgaa gctgaatccc
    18241 cttagaagca tcccagcggt ctttcagcgc gtttctcaaa tcggattttt tacccgttat
    18301 atgaccgatt gcagccccga tgttcgccgc acgggaaaaa cgcaccgcct ccgtatcaaa
    18361 atcggcaatg ccgcccttgc ccgcatcttt atcttgcaaa atctgcaaca ggcgtttatc
    18421 atcaatatct gataaaggcg attgattgga aatctccgca ctagataagc gagaggtcat
    18481 ttccaaaagg tcgccttttt ctattctcgt caatttatgg tcatagtatt tgtcgcctgt
    18541 tgtcgcaacg gcgattgccg cttttaccgt gtaatcagtc ccaccgatat tcaagcctgc
    18601 aacataatat tcatactctt gaatatcggg atttttggct ttgtcttcat tcggcaaagt
    18661 atcgatataa atcgacttct cgataatctg cggaatggcg gctatgcttt gcaaatgttc
    18721 agggttactg acatcgtgat gcaacacctc cgttatgccc gcccgtccga gtgaaatttc
    18781 acgccccgtg tccttattca cataagaccc gcgcaacgat ttcccatact ccattgcacg
    18841 gcgacgatac tggcgcaaat catcacccgc ctcaatctcc ctgcccgaaa tgcggatggt
    18901 ttccgcacgg cgcagcttct caaggctttc cgacttggaa cgttccgccg cccggctgtt
    18961 gcgtgtgccg tccgccgtat tgacagattc atccttcgcg cgtacattag gcgtatcaga
    19021 cgctcccaca tcacgacgcg caggtgtagg cgtggtgtgc gtagcggcgg aggtatcaaa
    19081 tcctaccccg tcggcaccgt tctgatacag ctcatagccg gtaatcaacc aagcgttgtt
    19141 tccggcattt ttaataaggt tcgcttcata accgttgtgc cgtagctgca aacgttcgga
    19201 attaccgtta acaacgtgtt tgtaaacctt gcctcgtgcg atagcttcaa caatatcatt
    19261 agtcaacatc cccacaactt cgtcacggcc catcccgtct ttgcgcatac ggctttcaat
    19321 cacgtgcgcc aagcccatcg cgcccttagt cttaccgttc ggcaaaaccc tgcccgtacc
    19381 gccccaaacg aaatcaatcc aaccgatgtc gttgcggtac atcgcacggc gcacatccgc
    19441 cttttccacc aacgcccgat tcatcgccgc ccgtccgcgc cggatattcg cgtccgtatc
    19501 cttcatcgcg ctcctgctag gcttcacacc gtccgaagat gcatcccgcg ccccttccgc
    19561 attcccgtgc aaatccgcca gcatcgcaaa cacatccgcg tccgacatcg cgccgtcggc
    19621 cttacccgcc agccgttgca gcacacggcg caataccgcg ccgatacgcg ccaacacgcc
    19681 cgcaatgccg cgtttcaaac cgttgccgac cttcacgccg taacggtttt caatgcccga
    19741 ccaatcgccg gtacgctgcg ccgcataaag ctccacgacc gcctcttcca ccgcggcaga
    19801 ccgcaccgaa gccgccgcat cgcccgtacc ttcgcgctct tcctgtaccg catccgcaat
    19861 acgccgaatc aggctgttgc cgtccgcacg ttccaattcc gcacggtact tggcaaagcc
    19921 atccgccgca aagcccctgt gccccagctc gtgccaagcc acccataccg cacgttcggg
    19981 cgtaaggttt tccgccacca gcgtaatttt gcccgtcctg ccgtcaaacc agccctccac
    20041 acgctccgac aacagcatct gcgccttgtc cggcgctgtt tccgacacgg cggcgacatc
    20101 cacctgttcc gccagtctgc ctatactttc ggcaaggcgt tttttgatgc tttcggcgtg
    20161 ttgttgacct gtatcagttt tttcagattt tctgtagcct tgcagattct tttcagttag
    20221 aatcttctta cggcttgtgt tcaagctcgg caggcggggc aattggagcc ccgaacgacc
    20281 gagaagttca cgagcctttt tactgtccac aaattccgta ttgccattag ccaaatcgcg
    20341 ccaaatgtcg cggaaagtct gttgcggatt gccatctttg gcatatccgt tgactaaaac
    20401 gtgaatgtcc aagccacgac tgttcggctc taccaccaga tacacgggcg caccgtctat
    20461 cagttcgtca ggcacaaata caagacgctt gtcatgcgtt ttcgacttca gtacagcaac
    20521 aggattgtcc agccattttg gaactttttt ccattgttgc gccgtcattt caggatggtt
    20581 tttccgattc agcacaacct tggattcatt gagttcaaca ggcataccac cataacccaa
    20641 catatcgagc aggtctgact tgtccaaaac ccttacgccc tttagatttg ccttcgcacc
    20701 accgaacagc gcgtcaatac ggttttcaaa cgccgacttc gtctgttccg aacggctgaa
    20761 ccttagccct tcagacggcg tgtcttccgt tttaccgcct tgcgtcttgt atttcagcag
    20821 ctttttgagt acactggcaa tgtatcctga attgaccttc cccgtttcgc ggctgatttc
    20881 agccgtctcg gtatcactgg cattcaggat actttctttt gtagccacgc tgtgcaggta
    20941 catctgactg ccgttgccgg tatttcgggc aagcagcgta accacatcat ctttcccctc
    21001 gatttccaca ggcgcactga tatacacata gcgcgtaccg ttttcagggt tcacgccctc
    21061 gtggataacc acgccctgac tgattacatc gggaatcgcg gcaaacgctt cagccttaaa
    21121 cgggttcata ccgtgtgcaa tcgaatcacg gacagaacgt tcgtttaaca ggatttctcc
    21181 cgcctcagga ttaaccgcct ttccgcccgc cttatcgaac agcgcgaccg cccactcgcg
    21241 caacgccttg ttttccccgg gcgcggtacg ctctttcacg gtataaacag gctctccctg
    21301 caacacccgc gccttgcgtc gtgcctcttg cggattgtcc aaagccgttt tcacaagcgt
    21361 ttcaggcttg gcagccttat ccgaagacgg cttttcattt gccgcatttt ttgcaacggc
    21421 cgccttgcct gcaacaggcg tatcttttgc ccgggcagac tgcgccttgc ccgccgattt
    21481 cccggcagaa gcagcggcat cgcgcgaccg cgccatcacc ttggccaacg cccttccttc
    21541 ctgcgtcaga cgataatccc aacccgactt ggacggcacg gcttccaaca aaccgcccgc
    21601 ctgcaaggtt tccgcctcct tacccttcaa ataacggcgg ctgtcgaaga caaggccgtc
    21661 agaatgggaa gaaccggcca tatcggcaaa ctcctccaac atcccgcctt tacccagcct
    21721 gtccagcaca cccgcaagcg catcaatatg ctgacggttc gtatgctcga aacggtattc
    21781 gccgtccccc aaaggcacac ccgccttttc cagcttgcgc ctgtccgccg ccaaacggcg
    21841 cgtaaaatcc gccgccgcat ccgccatccg tttcttaggc gataccttga ctgccgattc
    21901 cgcaacctgc ggattcagca ccggcaacac cgtatcgggg cgtttgcccg caacaggcgc
    21961 acgtttcccg ccgtccgttt cggcaaggtt ttcagacgcg gaaagaccgg gcgcggcacc
    22021 ttccgcgtgc gcccgaacct gtaagttttg ccgatgggtt ggcgcattgc cggacgcaac
    22081 ggcggcggac tcgggcaaac cctgcaaggc agccgcattt gcatccgccc ccgtatcagg
    22141 ggtcaaaccc ctgccgcctt gggtcaaacc ctgccgcaca tcctgccgca catcctgcgc
    22201 caaaccctta tcttccgtgg gtaaagcccg gggtaaaccc ctgccgccgc caacaacagc
    22261 ctcagacgcg ccatcatccg gcaacgccgt ccgaacccct tgcaaacgct ccccctcacg
    22321 gctcaacagc ggcacgcgcc aaccgttgtt gcggcggtaa ccgtaaaccg cacggttaag
    22381 cctgtccaac acatccgccg attccggatg ttccgcacgg taagcgcgta caaccctgac
    22441 cgattccgcc aaacccgcag gataatgtcc gcgcatatcc gccatacccg cctcttggcg
    22501 cataatcccg tccgccgtat agtccgccac cgtctgtgca tgacggcgca ccgtttccgg
    22561 cagcgcgtca aaccgcctac gcgattcctt attcaacgca tcggcaggga cggaaaaccc
    22621 ttttttagga acaggcacgg catccgccat acgcacgggc ggcggcagat acccctgttt
    22681 ggcttcagaa gccgcctgat aatccgcctc aatccgcgcg tgatacgcct cctcgatttt
    22741 ttggcgcaca ggctcggcga tttgcccgaa atccgcaacg cccttaatat caaagcccgc
    22801 ctccttcgcc aaagccttgc gcccccgagt atccaaaccg tcgaaatatt gacccgcagg
    22861 cagcggcgcg acacgcgcaa tcccgcccga agcagcggcg gcagaacgtc ccgcaccacc
    22921 cgacgcaacc gaagccgcac cgccggaacg cacattaccg taagcggacg aaacaccgcc
    22981 cctgtccgca tccgccgacc gtccggacag cacatcatcc gcaaccgtcg gcgtttccac
    23041 atcgtcagac ggcacagacg gcacagccgc gcccgaatcc accgccaaag cagcggccgc
    23101 agaaatcgca ccgtcatccg gatcaatccc catcgcctcc gacggcttag cccgatattt
    23161 ggccgccatg tccgcatact gacgcgccaa accatccgac attacgcccg tattcaaaaa
    23221 ttccgcctca tcctccgacg gaatacccga aacatccggc tgtttttcca tctcgcgcgc
    23281 caattcctcc gcgtggggcg tattgccgtc ggcagggctg ttgaaaaact gctccgcctc
    23341 cttctgcttc gtacggtcca caaactgctg caaagtcaga tacaactgat cgtgatgcgc
    23401 cgtatcaaaa cccgcgccgc cctgttccac gccgccgtcc agagccgcct ccaccgccgc
    23461 acctaacgcc tgcgcctctt ccggggtcag cttgcccgcc ttcctattgc cctcaatatt
    23521 ccccttcgca tccgccaacg catccgcctt ctgttcggcc gacaacctaa gcgggtcgct
    23581 tatcgccgca taagccttcg ttccgacaaa ctccaccgca gaatgcccca aagaagagaa
    23641 agcctccaaa gccgccccct tctcgtccca ttccccgttt gccagccccg tacccaaata
    23701 ctcgcccgca aactcactgg ccggttccag cgcaaaagcc gccgcgtgcc gtgccgcacc
    23761 gcccaaaccg cccttggcag actccttcgc cagtgccgca aattcaggcg ttcccttagc
    23821 cgccgcaacc gccgccttat ccgccgccgc aacacccatt ttcttcaatg cggctttctc
    23881 ggcggccttc ttgcccatat tcaaaatact gccgccaagt ttcatcgtcg ccatatccac
    23941 cgcgccgacc accgcgccct tgaccgccgc atttttcaac gcacccggcg caccgcggtc
    24001 gataaacgcc atcatcgcat ccttgtccgt aggatcgacg cccgccgcct ctgccgccct
    24061 gtccagctgc tcgccgtatt ccatcagcgt attgcccaag gtcgcgcctg cataagcacc
    24121 gacagccgtg ttaccgcccg ccaaagtgcc caaaataccg ccgacagttg tcgtagcaag
    24181 cggaacaagc gcattaggcg cctgctccag cgtcgcaccc cccatctcgc ccacatcttc
    24241 caagacaccg tccaaaccct tttcgcgcca gtctttctta agctcctccc acacattcga
    24301 cagaccgccg ccgacaccct cgcccttttc ccaagcctcg cccagctccc ttcgcgcttg
    24361 agatttacgc ccttcctgaa tcttccgata atccatatcc tcagccttca gttggccgag
    24421 ttcctcttta tcgcccgtca aatcattcca cgccatacgc gccgaacgca tagaattttt
    24481 aaacccgcgc gtcaacgccg tatcgtcggc agccgcccac gcatcctttt gcgtaaagcc
    24541 gcgatacttc ttcaaacccc cgacaagctg cccgtgttcc aacggatcgt atctgaccaa
    24601 atcactcatt gctcaaccct cttctcaccc ctatcattct cataaatgat tttaccgtcg
    24661 gccgttttct caccgttcgg cttaaagccc aacgccgcat aacgcttctc gcgctctccc
    24721 gcaaactcca catccaaagg cttgcccgtc cgcaaatcaa caatcccctc gcgcttcgcc
    24781 gtcaagcccg tattcgggtc ggtaatctca taactgattg tcttaaacac attcgggtca
    24841 aagcccttct cctctttctg ctcaaacttc ccattaagcg catcaatctg ccgcgcaatc
    24901 tccccacgtt gcccgtccga cttcgcaaaa ccataagccc gcgtcaaatc agcgatacgc
    24961 ttcctattct caagctccaa cgcatccgcc tcacgcttca tctcaaaccc ctgccgcttc
    25021 atccccagct cggcctgctg cacatcaaac gccctgtccg cattcctctg cgccatctca
    25081 cggttcaacg caccctcatt cgccttaacc gccgcatcaa accgcccctt ctgcaacatc
    25141 gcctcacggt tcgccgcatt ctccgccgcc gccgccgcca gcctttccct gcccaattcc
    25201 ccgtccgccg catttttatt atgttccgcc acaatccccc gcgccgcatt aagctgcgcc
    25261 gccgtcaact gaccgttcaa agcccccgca tgaggcgtcg tcgccctctt ataaagctcc
    25321 tgctcctccg gcgacagcgc atccggcttg gcagacacca aaggacgctg cgcatcaaaa
    25381 tcacgcacca gcttaccgcc ccgatacaca ttcccgtcaa agcccaaaga cggcagcgtc
    25441 tgtccgcccg catccgtcga gcgcgtatca cgccgcgcaa aatccgcctc agcccgcgca
    25501 tcgggggaaa gcaccttatc gccgcccgaa aaccccaaac cggcatccgc atcccgttca
    25561 aaagcacccg cctgccgcaa ccgccgcgcc ccatcctcaa tattgttacg gctcaagtcc
    25621 atcacattcc gataacgcgc cagcccatcc tcataattcg caacagtctg ccgcaccaca
    25681 tcggcacgac gcaccagcgc cgcctcccgc tcctgcgcct ccatctcctc cctagtcttc
    25741 tcttttttct ccttttcctt catctccgaa aaatccccgg cagtcggctc aaacaaaccc
    25801 atcacaaacc ctccaaacaa ataaagtcca aaataagcaa aaacccaaac ccaatcaaac
    25861 gcctaaagtt aagaacaaac aaaaatctca accgcaaacc aattaacaaa caacaagtta
    25921 cacaaaaaaa cacaccgaca agccattttc atgggcaaat aaaaaaccgc ctttgtgtaa
    25981 agcggttgca aaaaagcctt tcaataaaaa tgcggaaacg gttttttatt tgcgtttccg
    26041 cataatacaa acccacacac aaaaaaatca ttttccgttt ttggcaagcc tcaaaacatc
    26101 attcccccac cgttccagcg catccttacg ctcctcccaa agctccgagc ggttgtaagc
    26161 cgtttgcgtt ctcgtcccca acgaatgggc aagcagcttt tccgccacat cattatccac
    26221 cttcagaacc tcgcgcaaat aagtacgcgc aagcgagcgc aaaccgtgcg ccgtcgtatc
    26281 cagccccgcc cgctgcatcg ccacgcgcgg actttcctta ttgatatgcc cttgaaaatt
    26341 cacactttca aacacaaacc gcccgttcac attcaacgcc cgcgcctgat ccaacaccca
    26401 ctgcatcgcc cgcgacagcg gcacatcatg cccccgccgc tcctttttcc gttcggcggg
    26461 gatacgccaa acctccccgt ccaactccga ccactccatc aacgccgcct cctgaatccg
    26521 cgtcattgtc aacagcagcc aatagataca aagccgcgtt acaggatgga tacgcaccct
    26581 gcccgcataa accgcaaatt cattgcgctg ctccaaaaaa tcaatcagac gcggcaattc
    26641 agacggactc aacgccgcca tattccccgt tttcgcccgt tcaaacacac ccgaaccgat
    26701 ttgcgcgaca gggttgattt ccatcattcc cgaaccgacc gcaaacgcaa acaccatctt
    26761 cagactgttt ttcgttttgc gcaacgtatc aacgataccg cgcgcctcca tcacacgcag
    26821 acagccgacc acatccgccg tcctgacttg acgaatatca agattgccga tagccggaaa
    26881 aacccaccgt tcaaaattcc gcataacctg tccggcatac ttttcagacc gacccttcga
    26941 ccaacgcaca aaccaatcac gcgccacctt ctcaaaagca aaatccgccc gcaccttctt
    27001 attgacgaca ttttccccgt gcgcccgttt tcggcgcacc tcctcccgcc attcccgcgc
    27061 atcggccagc gaaaaatcag gataccgccc cagcgaaatt gtctgctgcc ttccatcctg
    27121 cacaaacgac agcttccaac tccgcccgcc cgtcgggtag atccacaaag ccaacccgcc
    27181 gccgtccgac aacttataca gcttatcgcg cggcttcgca tttttaacct gatttgccga
    27241 cagcggcgta atgattttcg ccattatggt aatttcccac ttatccaaaa attaccatat
    27301 acattaccat aaaaaaaatg caataagaag ataccgcaag attgcaaatg atttactttg
    27361 acgcgttttg atagggcaaa ccgatgattt ttatatggta attgacacaa cttgaacaaa
    27421 aaagaaccgc cctgattcag ggcggttatg ttttctggcg gaaacggtgg gattcgaacc
    27481 cacggaggat ttgcaccctc agcggatttc gagtccgctg cattcagcct ctctgccacc
    27541 tttccgataa tgaagtaaaa ccaaataaaa atgccgatgc tttatgctac ttcattttta
    27601 tttggttttt gacctcaagg attggcggaa gcggtgagat tcgaactcac ggagggctat
    27661 caaccctcga cggttttcaa gaccgttgca ttaaaccgct ctgccacgct tccgtcttct
    27721 tgaagattag gaataataat gaaatttata cattttgcca agcacttttt tcggaaaata
    27781 cttaatttat tgttttcact ttatttgttc cgagcgcata aacacccatt cgctttcatt
    27841 ggaagccgcg tcattgaatg cgtagccttc gtaattaaaa tttttcagca tttcagggtc
    27901 ggtaatatga tgttctgccg catagcgcac cattaatccg cgtgcacgct tggcatagaa
    27961 actgataatt ttatatttgc cgtttttttc gtctttaaat atcggggtaa tcagccgcgt
    28021 ccgaagtttt ttcgtgttga cggacttgaa atattcctgt gaggcaaggt tgacaagcgt
    28081 attgctgcct gcttgggcaa gcgtgtcatt taaaaggttg gtaatgatgt ctccccaaaa
    28141 ctcatacaaa ttcttgccgc gcaaattggc aaatgccgtc cccatttcca aacggtaggg
    28201 ctgtatcagg tctaacgggc gaagaagacc gtacagaccg gacagcaggc ggacatggtt
    28261 ttgcagatag cgtatctgtc caatatccaa tgtgtttgca tccatacctt cgtaaacatc
    28321 gccgttgaac ataaagaccg cctgtttggc gttttccggc gtaaacggcg tgtgccattc
    28381 tgcattgcgc tgcgcgttta agagggcaat tttgtcggaa acgtgcatca gttcggcaat
    28441 ctgttgcaac gcaagctcgc gcagctgctg cattagaatt tcggactctg ccagcaggtc
    28501 gggttgggta aactcgctga cagaggcagg gtctttttca ttaaggttct tcgcagggga
    28561 aaggacaaaa aacataatct gctcggtatt gtgtcaaaca cggggatttt aagtttaaac
    28621 gtgtttgcgg gcaagccgat taaaagtgaa gtatagtgga ttaaatttaa accagtacag
    28681 cgttgcctcg ccttgccgta ctatctgtac tgtcttcggc ttcgtcgcct tgtcctgatt
    28741 tttgttaatc cactataaat accgttatag caaattcaac cgtaaaatat tgaaaaatac
    28801 cgtctgaaca aaagttcaga cggcattttt tttattcttt accgatatag ggaaacaatg
    28861 gtttccgcag ctttgacaca agttgcgaca tcggcaacca aagcgatacg cacataacct
    28921 tccccgggat tgccctgttc ggtatcccgt gccaaaaagc gtccgggcaa tacttggata
    28981 gcggcttttt gccataaatt gcgtgcaaat gccaaatcgt cgccatcggg aactttcaac
    29041 cagatgtaaa acgaggcatc cggtaattta acatcaaata cctgttgcaa aatgggaata
    29101 acgcgctcaa atttttcctg atacaggcgg cggttgtcga taacgtgctg ttcgtcatcc
    29161 caagcggcaa tactggcacg ctgcacggga atgctcattg cgctgccgtg ataggttctg
    29221 taaagcagaa agtttttaag cagttcggca tcgccggcga caaaaccgga acgcaaacct
    29281 ggaacattgg accgcttgga caaactggtg aacataagca gtttttgcct gcttcgaccc
    29341 aactgtgcag cggcctgcag gcagcctaaa ggtttgttgc cgtcgaaata gatttcggaa
    29401 tagcattcat ccgaggcaat aatgaaacca tatttatcct gtaaatcaaa aatttctttc
    29461 catccgtcca aatccagcac gctgccgctg gggttgttgg gcgagcagac gaacaccagt
    29521 ttggtgcgtt tccaaacctc ttcggaaata ctacgccaat cggggttgaa agacggcgcg
    29581 gggcaattgg caaaatggat ttcaccgccg cccaaaagtg tcgcaccttc gtaaatctga
    29641 taaaagggat tcgggctgac aattgcgggt ttgatgccgt ctgaaacagg gttcaacacg
    29701 gtttgaacaa aagaaaacaa cgcctcccta ctgcctaaaa ccggcagaat ttcattatcc
    29761 gcatccactg tcaagccatc gtaacggcgt tttaaccagt ttgcacacgc ctgacgcagt
    29821 tcaggcagac cggccgtcag cggatatttt tccaactcgt gcaatgaggc ggtcagcgca
    29881 tctgtaatga ctttcggtgt tggatgtttc ggttcgccga tatgcagtgg gacggcttcc
    29941 agaccttcgg gggcggaaat gccctgcatc gcttcacgca gtcgggcaaa gggatagggt
    30001 ttgagttggt tgagtaaggt attcataatg ttctccggtt gtcggtatat cggaagatat
    30061 gccgtctgaa cctgacggca cccatcagac ggcattttat attcaatgca aacccgtact
    30121 gccgaaaccg ccttctccac ggctgcttcc gacaaattct tcgacacgtt tgaaacgcgc
    30181 ctgcacgacc ggaacaataa ccatctgcgc gatacgctcg aacggtttga cagtaaacgg
    30241 ctcgctgctt ctgttccata acgacacctt caattcccct tgataatcgg agtcaatcaa
    30301 accgaccaaa ttgcccaaaa cgatgccgtg tttatgcccc aagccggaac ggggcagcaa
    30361 aacggcggca tatgcgggat tcgccaaata aattaccaaa cccgtcggca caagaaacgt
    30421 ttcacccggc ttcaaaacga cttcctcatc caaacaggcg cgcaaatcta aacctgcaga
    30481 accctccgtt gcataggcag ggataaaatc cgccatcctc tcgtccaaca ctttcatttc
    30541 tacttcaata ttcatcgtct tgctcctgca cacggccggt tttaaagatg ggagattata
    30601 cacaagccgt gtttacgctg aaacacggca aatcggtttt gttttgaaat ccgttgccag
    30661 tgtataatgc ccgcccttcc agttatgaag acgaacatta tgcacacacc ttccccgcat
    30721 aacgaattta tacgcgccat caaagaaagt tcgcccatac ttatcggact tctgccttgg
    30781 gcattaatac tcggtatgca gggtggacaa aaaggcatga gctggctgga aatgttgttg
    30841 atgaccagta tgaacttcgc cggcggctcc gagtttgcca cggtcaacct gtgggcggaa
    30901 cctctgccga tactgcttat cgccaccgta acctttatga ttaattctcg gcatatcctg
    30961 atgggggcgg cacttgcccc gcacctgaaa ggaataccgc tgaaaaaagc cgtacccgca
    31021 ctgtttttta tgtgtgatga aagctgggcg atggcattct ccgaaatcca aaaacggaaa
    31081 gcagccggtt tgcccgcatt caatatgc
//
LOCUS       DAXHRI010000007        28557 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXHRI010000007 DAXHRI010000000
VERSION     DAXHRI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.007"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            24..1106
                     /gene="mraY"
                     /locus_tag="ACFM75_000227"
     CDS             24..1106
                     /gene="mraY"
                     /locus_tag="ACFM75_000227"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC6254833.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQVVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            1221..1493
                     /locus_tag="ACFM75_000228"
     CDS             1221..1493
                     /locus_tag="ACFM75_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC6254834.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWLGKACQNGDQDGCDNDQRLKAGY"
     gene            1577..2914
                     /gene="murD"
                     /locus_tag="ACFM75_000229"
     CDS             1577..2914
                     /gene="murD"
                     /locus_tag="ACFM75_000229"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC6254835.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCDLN
                     MTDCATLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            3040..4365
                     /locus_tag="ACFM75_000230"
     CDS             3040..4365
                     /locus_tag="ACFM75_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC6254836.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            4369..5436
                     /gene="murG"
                     /locus_tag="ACFM75_000231"
     CDS             4369..5436
                     /gene="murG"
                     /locus_tag="ACFM75_000231"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC6254837.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVQAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            5591..7000
                     /gene="murC"
                     /locus_tag="ACFM75_000232"
     CDS             5591..7000
                     /gene="murC"
                     /locus_tag="ACFM75_000232"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC6254838.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            7113..8027
                     /locus_tag="ACFM75_000233"
     CDS             7113..8027
                     /locus_tag="ACFM75_000233"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC6254839.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGISYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            8017..8745
                     /locus_tag="ACFM75_000234"
     CDS             8017..8745
                     /locus_tag="ACFM75_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC6254840.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            8831..10075
                     /gene="ftsA"
                     /locus_tag="ACFM75_000235"
     CDS             8831..10075
                     /gene="ftsA"
                     /locus_tag="ACFM75_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC6254841.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREVGGGLLARLKRWIENSF"
     gene            10194..11372
                     /gene="ftsZ"
                     /locus_tag="ACFM75_000236"
     CDS             10194..11372
                     /gene="ftsZ"
                     /locus_tag="ACFM75_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC6254842.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVGFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            11511..12821
                     /locus_tag="ACFM75_000237"
     CDS             11511..12821
                     /locus_tag="ACFM75_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC6254843.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            13446..14321
                     /gene="prpB"
                     /locus_tag="ACFM75_000238"
     CDS             13446..14321
                     /gene="prpB"
                     /locus_tag="ACFM75_000238"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC6254844.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            14407..15561
                     /gene="prpC"
                     /locus_tag="ACFM75_000239"
     CDS             14407..15561
                     /gene="prpC"
                     /locus_tag="ACFM75_000239"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC6254845.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            15887..16831
                     /gene="gntB"
                     /locus_tag="ACFM75_000240"
     CDS             15887..16831
                     /gene="gntB"
                     /locus_tag="ACFM75_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis L-arginine gamma (S)
                     hydroxylase"
                     /protein_id="HFC6254846.1"
                     /translation="MYHKKFDKEILLKIKELYRYDNWHAVLAILYDYLIIIFSIFLSE
                     QSYWFLPLTILLIGSRQRALATILHEASHSALTKNKKFGQILGTYFSGYLIFQSWNSY
                     CVSHVKNHHPKLGTDSDPDYKYYKDSGIFQTYSRIEFFWRFCVSQILCLKLFRNFKYL
                     IKNRLFSDITRTELFKLLLSQLILFVIGTYFVGYKFYVIYWLLPYLTAFQMITWFIEL
                     SEHYPMIQTAKNNLEASRNRFSHAIEHFFTGIHGENYHLIHHLFPAIPFWNLEKAHKI
                     LMKDFDYQEVNKDFGGIFFSKNNNAPMWKVLWSKYEAA"
     gene            16818..17729
                     /locus_tag="ACFM75_000241"
     CDS             16818..17729
                     /locus_tag="ACFM75_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6254847.1"
                     /translation="MKLRDLLMGIFLAVSAALLNATIGIFSKILMEQGLSVQHIAFLK
                     TLTGFVFISILLCRTGFTRQIADISRKKEAILPLLLKVAICAFFGIYTLFFFETTAYQ
                     YGNAANVVVVLMASAAVSALILDSILLDERICISSVVGVGLAVLGIAMISWTGEGSLG
                     LILNAALAGSGYGCFSVLIKKFGLNGGIYLTRILMFFGSIFLFIPSLEGIEDIHWQWS
                     FIPPLLALSLLPTILGFYCTTKALDYLSAAKVQVTELAEPLFAAVLAWLFLNEIPEGR
                     FFVGAILIIAGIVSINGLYRPLLKRIE"
     gene            17820..20426
                     /gene="acnD"
                     /locus_tag="ACFM75_000242"
     CDS             17820..20426
                     /gene="acnD"
                     /locus_tag="ACFM75_000242"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC6254848.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLASKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANCFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            20778..21947
                     /gene="prpF"
                     /locus_tag="ACFM75_000243"
     CDS             20778..21947
                     /gene="prpF"
                     /locus_tag="ACFM75_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC6254849.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            22361..23560
                     /locus_tag="ACFM75_000244"
     CDS             22361..23560
                     /locus_tag="ACFM75_000244"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6254850.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            23668..24288
                     /locus_tag="ACFM75_000245"
     CDS             23668..24288
                     /locus_tag="ACFM75_000245"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6254851.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(24415..24858)
                     /locus_tag="ACFM75_000246"
     CDS             complement(24415..24858)
                     /locus_tag="ACFM75_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC6254852.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            25032..25400
                     /locus_tag="ACFM75_000247"
     CDS             25032..25400
                     /locus_tag="ACFM75_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6254853.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            25445..26599
                     /locus_tag="ACFM75_000248"
     CDS             25445..26599
                     /locus_tag="ACFM75_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6254854.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(26656..27528)
                     /locus_tag="ACFM75_000249"
     CDS             complement(26656..27528)
                     /locus_tag="ACFM75_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC6254855.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATFPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            27613..28425
                     /locus_tag="ACFM75_000250"
     CDS             27613..28425
                     /locus_tag="ACFM75_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC6254856.1"
                     /translation="MDKIRVVAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         6608 a         7259 c         7891 g         6799 t
ORIGIN      
        1 acaaaacaaa aaaaggaagc cccatgtttt tatggctcgc acatttcagc aactggttaa
       61 ccggtctgaa tatttttcaa tacaccacat tccgcgccgt catggcggcg ttgaccgcct
      121 tggcgttttc cctgatgttc ggcccgtgga cgatacgcag gctgaccgcg ctcaaatgcg
      181 ggcaggtagt gcgtaccgac ggcccgcaaa cccacctcgt caaaaacggc acgccgacga
      241 tgggcggttc gctgattctg accgccatta ccgtgtccac tctgttgtgg ggcaactggg
      301 cgaacccgta tatctggatt ctcttgggcg tactgcttgc caccggtgcg ctcggttttt
      361 acgacgactg gcgcaaagtc gtctataaag accccaacgg cgtgtccgcc aaattcaaaa
      421 tggtgtggca gtcaagcgtt gccattatcg ccggtttggc attgttttac cttgccgcca
      481 attccgccaa caatattttg attgtcccgt tcttcaaaca aatcgccctg ccgctgggcg
      541 tggtcggctt tttggtgttg tcctacctga ccatcgtcgg cacatccaac gccgtcaacc
      601 tcaccgacgg cttggacggc cttgcgacct tccccgtcgt cctcgttgct gccggcctcg
      661 ccattttcgc ctacgtcagc ggacactacc aattttccca atacctccag cttccctatg
      721 tcgccggtgc gaacgaagtc gctatattct gtaccgccat gtgcggcgcg tgcctcggtt
      781 tcttgtggtt caacgcctat cccgcgcaag tctttatggg cgatgtcggc gcgctggcat
      841 tgggtgccgc gctcggtacc gttgccgtca tcgtccgcca agagtttgtt ttagtgatta
      901 tgggcggtct gttcgtcgta gaagctgtgt ccgttatgct tcaggtcggc tggtataaga
      961 aaaccaaaaa acgcatcttc ctgatggcgc ccatccatca ccactacgaa caaaaaggct
     1021 ggaaagaaac ccaagtcgtc gtccgctttt ggattatcac catcgtcttg gtgttgatcg
     1081 gtttgagtac cctcaaaatc cgctgaacct atgccgtctg aacatctttc agacggcatt
     1141 tgaacgcgca ataaacctgc ggcgacaatc cgcccagcct tatcgttaac ggcggcttat
     1201 actaaaacag aagtaaaacc atgaaacaga cagtcaaatg gcttgccgcc gccctgattg
     1261 ccttgggctt gaaccaagcg gtgtgggcgg atgacgtatc ggattttcgg gaaaacttgc
     1321 aggcggcagc acagggaaat gcagcagccc aatacaattt gggcgcaatg tattacaaag
     1381 gacgcggcgt gcgccaagac cttgtgctcg ctcaagaatg gcttggcaag gcttgtcaaa
     1441 acggagacca agacggctgc gacaatgacc aacgcctgaa ggcgggttat tgaacagctc
     1501 gcgatgccgt ctgaaagcgg cttgggcagg ggcggacatc tcctgctcaa tatgatttgt
     1561 tttaggacaa accaaaatga cttttcaaaa caaaaaaatc ctcgtcgccg gactcggcgg
     1621 tacgggtatt tccatgattg cctacctgcg caaaaacggc gcggaggttg ctgcgtatga
     1681 tgcggagctg aagccggaac gcgtgtcgca aatcggtaag atgtttgacg ggctggtgtt
     1741 ttatacgggc cgtctgaaag atgcgctgga caacggtttc gatattctgg cactcagccc
     1801 cggcatcagc gagcggcagc cggatatcga ggcgttcaag caaaacggcg gacgcgtgtt
     1861 gggcgacatc gaattgctgg cggacattgt gaaccgccga ggcgacaagg taattgcgat
     1921 taccggcagc aacggcaaaa ccacggtaac gagcctggtc ggctatctct gcatcaagtg
     1981 cgggctggat accgttatcg cgggcaatat cggcacgccg gttttggagg cggaatggca
     2041 gcgcgaaggc aaaaaggcgg acgtgtgggt gttggagctt tccagcttcc aactggaaaa
     2101 caccgaaagc ctgcgcccga ctgcggcgac ggtactgaac atttccgaag accatctcga
     2161 ccgctacgac gacttgctcg actacgcgca taccaaagcc aagattttcc gtggcgacgg
     2221 tgtgcaggtt ttgaatgcgg acgatgcgtt ctgccgtgcc atgaaacggg cagggcgcga
     2281 ggtaaaatgg ttttcgttgg aacacgaagc cgatttttgg ttggaacgcg agacaggccg
     2341 cctgaaacaa ggcaatgaag atttgattgc cacgcaagat attccgttgc aaggcttgca
     2401 caacgccacc aacgtgatgg ctgccgttgc cttgtgcgaa gctgtcggtt tgccgcgcga
     2461 agcattgctc gaacacgtca aaacctttca aggcctgccg caccgcgtgg aaaaaatcgg
     2521 cgagaaaaat ggcgtggtgt ttatcgatga cagcaaaggc acgaacgtcg gtgcgaccgc
     2581 cgccgcgatt gccggtttgc aaaatccgct cttcgtgatt ttaggcggca tgggcaaagg
     2641 gcaggacttc acgcccctgc gcgacgcgct tgccggcaag gcaaaaggcg tgttcctgat
     2701 tggtgtcgat gcgccgcaaa tccgccgcga tttggacggc tgcgatctga atatgaccga
     2761 ctgcgccact ttggaagaag cggttcagac ggcatatgcc caagccgaag cgggcgacat
     2821 cgtgttgctc agccccgcct gcgcaagctt cgatatgttt aaaggctatg cgcaccgttc
     2881 ggaagtgttt atcggggcgt ttaaggcttt gtgataccgt ctgaaacgca aacgccgtca
     2941 ttgttgggcg gcaagtaaag atttagaata ccgatttggg atgtatcgta tgttcggacg
     3001 gcattgtctg ccgtctgaaa tttttgccct ttgcggcagg tgcaaacaga ctggcaggtg
     3061 gtttttttga agatttcgga agtattggta aaagtgggcg acggtgtcca cactctgctg
     3121 ctcgacaggc cgattgtgcg cgacggcagg aaattcgacg cgccgctttt gtggatggtg
     3181 gtgctgatga cggcgttcag cctgctgatg atttattcgg cttctgtgta tttggcatca
     3241 aaagaaggcg gcgatcagtt tttctatttg accagacagg cggggttcgt cgttgccggc
     3301 ttgatagcga gcggtttttt atggtttctt tgcaggatga ggacatggcg gcggcttgtg
     3361 ccgtggattt ttgccctatc cggcctgttg ctggtagtcg tattgattgc cgggcgcgaa
     3421 atcaatggcg cgacccgttg gatacctttg ggtccgttga atttccagcc gaccgagctg
     3481 ttcaagctgg cggtcatcct ttatttggca agcctgttca cgcgccgtga agaagtgttg
     3541 cgcagcatgg aaagtttggg ttggcagtcg atttggcggg ggacggccaa tctgatcatg
     3601 tccgccacca atccgcaggc acgtcgtgaa acattagaaa tgtacggccg tttccgggcg
     3661 atcatcctgc cgattatgct ggtggcgttc ggtttggtgc tgataatggt acagccggat
     3721 ttcggttcgt ttgtcgtcat taccgtcatt gccgttggaa tgctgttttt ggcaggattg
     3781 ccgtggaaat attttttcgt cctggtaggc agcgtcttgg gcgggatggt gctgatgatt
     3841 accgccgctc cctaccgtgt gcagcgggta gtggcatttt tggacccgtg gaaagacccg
     3901 cagggtgccg gctaccagct tacccactct ctgatggcaa tcgggcgcgg agagtggttc
     3961 ggtatgggtt tgggtgcgag tttgagcaaa cgcggctttc tgccggaagc gcataccgat
     4021 tttatttttg ccatcatcgc cgaagaattc ggtttcttcg gtatgtgcgt gctgatattc
     4081 tgttacggct ggctggtggt gcgggcgttt tccatcggca agcagtcgcg cgatttgggt
     4141 ttgactttca acgcctatat cgcttcgggt atcggcattt ggatcggtat ccaaagtttc
     4201 ttcaatatcg gtgtgaacat cggtgctttg ccgaccaaag gtctgacgct gccgttgatg
     4261 tcctatggcg gttcgtcagt ctttttcatg ctgatcagca tgatgctgct gttgcgtata
     4321 gattatgaaa accgccggaa aatgcgcggt tatcgggtgg agtaaatcat gggcggtaaa
     4381 acctttatgc tgatggcggg cggaacgggc ggacatattt tccccgcgct ggcggtggcg
     4441 gattcattgc gcgcgcgcgg ccatcatgtg atttggctgg gcagcaagga ttcgatggaa
     4501 gagcgtatcg tgccgcaata cggcatacgc ttggaaacgc tggcgattaa aggcgtgcgc
     4561 ggcaacggca tcaaacgcaa gctgatgctg ccgtttactt tgtatcaaac cgtccgcgaa
     4621 gcgcagcgga ttatccgcaa acaccgtgtc gagtgcgtca tcggcttcgg cggcttcgtt
     4681 accttccccg gcggtttggc ggcgaagtta ttaggcgtgc cgattgtgat tcacgagcaa
     4741 aacgccgtgg caggtttgtc caaccgccac ctgtcgcgct gggcgaagcg ggtgttgtac
     4801 gcttttccga aagcgttcag ccacgaaggc ggcttggtcg gcaaccccgt ccgcgccgat
     4861 attagcaacc tgcccgtgcc tgccgaacgc ttccaagggc gtgaaggccg tctgaaaatt
     4921 ttggtggtcg gcggcagttt gggcgcggac gttttgaaca aaaccgtacc gcaggcattg
     4981 gctttgctgc ccgacaatgc gcgtccgcag atgtaccacc aatcgggacg gggcaagctg
     5041 ggcagcttgc aggcggatta cgacgcgctg ggcgtgcaag cggaatgcgt ggaatttatt
     5101 accgatatgg tgtccgccta ccgcgatgcc gatttggtga tttgccgtgc cggcgcgctg
     5161 acgattgccg agttgacggc ggcgggattg ggtgcgttgt tagtgccgta tcctcacgcc
     5221 gttgatgacc atcaaaccgc caacgcgcgt tttatggtgc aggcggaggc gggattgctg
     5281 ttgccgcaaa cccagttgac ggcggaaaaa ctcgccgaaa tcctcggcag cctgaaccgc
     5341 gaaaaatgtc tcaaatgggc ggaaaacgcc cgtacgttgg cactgccgca cagtgcggac
     5401 gacgttgccg aagccgcgat tgcgtgtgcg gcgtaaactg ccgaaccatg ccgtctgaaa
     5461 agccgttcag acagcatgga tgttttttat ttcaatccgc tatatatttg tcagaaaact
     5521 atggcgtgca aacggtcagc cctttaaaat aacgccttta cgcatcgaaa atccaccgga
     5581 acgcaacatt atgatgaaaa atcgagtgac caacatccat tttgtcggta tcggcggcgt
     5641 cggcatgagc ggcatcgccg aagtcttgca caatttgggt tttaaagttt ccggttcgga
     5701 tcaggcgcga aatgccgcta ccgagcattt gggcagcctg ggcattcaag tttatcccgg
     5761 ccataccgca gaacacgtta acggcgcgga tgttgtcgtt acctctaccg ccgtcaaaaa
     5821 agaaaatccc gaagttgtcg ctgcgttgga gcagcaaatt cccgttattc cgcgcgccct
     5881 gatgttggcg gagttgatgc gcttccgtga cggcatcgcc attgccggca cgcacggcaa
     5941 aaccacgacc accagcctga ccgcctccat cctcggcgcg gcaggacttg acccgacttt
     6001 cgttatcggc ggcaaactca acgccgcagg caccaacgcc cgcttgggca aaggcgaata
     6061 catcgttgcc gaagccgacg agtcggatgc atcctttctg cacctgacac cgattatgtc
     6121 cgtcgttacc aatatcgacg aagaccatat ggatacctac gggcacagcg tcgaaaaact
     6181 gcatcaggcg tttatcgatt tcatccaccg tatgcccttc tacggcaaag cctttttgtg
     6241 tattgacagc gaacacgtcc gcgcgatttt gcccaaagtg agcaaacctt atgctactta
     6301 cggtttggac gataccgccg acatctacgc caccgacatc gaaaacgtcg gcgcgcaaat
     6361 gaaattcacc gtccatgttc aaatgaaagg acatgagcag gggtcgtttg aagtcgtgct
     6421 gaatatgccc ggcagacaca acgtgctgaa cgcattggca gccatcggcg tggcgctgga
     6481 agtcggcgca tcggttgaag cgatccaaaa aggcttgctc ggctttgaag gtgtcggccg
     6541 ccgcttccaa aaatacggcg acatcaagtt gccaaacggc gggaccgcgc tcttggtgga
     6601 cgactacgga caccaccccg tcgaaatggc ggcgaccctt tccgccgcac gcggcgcgta
     6661 tccggaaaaa cgtttggtac tcgccttcca gccgcaccgc tatacccgca cgcgcgattt
     6721 gtttgaagac tttaccaaag tcctcaatac cgttgacgcg ctggtgctga ccgaagttta
     6781 tgccgccggt gaagagccga ttgccgccgc cgattcccgc gctcttgccc gcgccatccg
     6841 cgtgttgggc aaactcgagc cgatttactg cgaaaacgtt gccgatctgc ccgaaatgct
     6901 gttgaacgtt ttgcaggacg gcgacatcgt gttgaatatg ggcgcgggaa gcatcaaccg
     6961 cgtccccgcc gcgctgctgg aattgtcgaa acagatttga ggcacacccg cctgacagac
     7021 ggaacatcat ataaaggtcg tctgaaaccg caaatcaggt ttcagacgac ctctggcaac
     7081 aagcataaag caatcaggaa agaacaaaaa caatgcagaa ttttggcaaa gtggccgtat
     7141 tgatgggcgg tttttccagc gaacgagaaa tctcgctgga cagcggcacc gccattttga
     7201 atgctttaaa aagcaaaggc atagacgcat acgccttcga tcctaaagaa accccattgt
     7261 ctgaattgaa ggcacaaggt tttcagacgg cattcaacat ccttcacggt acttacggcg
     7321 aagacggggc ggttcagggt gcattggaac tgttgggcat ttcctatacc ggcagcggtg
     7381 tcgccgcatc cgccatcggc atggacaaat accgctgcaa actgatttgg caggcattgg
     7441 gattgcccgt tcccgagttc gccgtcctgc acgacgacac tgatttcgat gccgtcgaag
     7501 aaaaattggg cctgccgatg tttgtgaaac cggcggccga aggcagcagc gtcggtgtgg
     7561 taaaagtcaa agaaaaaggc cgtctgaaaa gcgtttacga agaattgaaa caccttcagg
     7621 gcgaaatcat tgccgaacgt tttatcggcg gcggcgaata ttcctgcccc gtcctgaacg
     7681 gcaaagggct gcccggcata cacatcattc ccgcaaccga gttttacgac tacgaagcca
     7741 agtacaaccg caacgacacc atttatcaat gtccttcgga agatttgacc gaagccgaag
     7801 aaagcctgat gcgcgaactg gcggttcgcg gcgcgcaggc aatcggtgcg gaaggctgcg
     7861 tgcgcgtcga tttcctcaaa gataccgacg gcaaactcta tctgttggaa atcaacaccc
     7921 tgcccggtat gacgagccat agtttagtac cgaaatccgc tgccgttacg ggcgtgggtt
     7981 ttgccgattt atgtattgaa attttgaaga ccgcacatgt gggataatgc cgaagcgatg
     8041 gaacggctga cgcgctggct gcttgtcatg atggcgatgc tgcttgctgc gtccgggctg
     8101 gtttggtttt acaattcgaa tcatctgccc gtcaagcagg tgtcgctgaa gggcaacctg
     8161 gtttattccg ataagaagac attgggcagt ttggcgaaag aatacatcca tgggaatatt
     8221 ttgaggacgg acatcaatgg cgcacaggaa gcctaccgcc ggtatccgtg gattgcgtcg
     8281 gtcatggtgc gccgccgttt tcccgatacg gttgaggtcg tcctgaccga gcgcaagccg
     8341 gtcgcgcgtt ggggtgacca tgccttggtg gacggcgaag gcaatgtttt tgaagcccgc
     8401 ttggacagac ccggaatgcc ggtattcaga ggcgcggaag gaacgtctgc cgaaatgctc
     8461 cgccgttatg acgaattttc gactgttttg gcaaaacagg gtttgggcat caaagagatg
     8521 acctatacgg cacgttcggc gtggattgtc gttttggaca acggcatcac cgtcaggctc
     8581 ggacgggaaa acgagatgaa acgcctccgg ctttttaccg aagcgtggca gcatctgttg
     8641 cgtaaaaata aaaatcggtt atcctatgtg gatatgaggt ataaggacgg attttcagtc
     8701 cgctatgctt ccgacggttt acccgaaaaa gaatccgaag aatagtggga acaggtatcg
     8761 gacagattac ggccgtgccg tctgaaacgg tgcgacgcaa atttcaatca gttttaagag
     8821 cagacgaaca atggaacagc agcaaagata catcagcgta ctggatatcg gtacgtctaa
     8881 agtcctcgca ctgatcgggg aagttcaaga tgacgacaaa atcaacatcg tcggtttggg
     8941 gcaggctcct tcacggggct tgcgcgcggg catggtaacc aatatcgatg ccaccgtcca
     9001 agccatcagg caggcggtca atgatgccga gctgatggcg gataccaaaa ttacccacgt
     9061 taccacaggt atcgcaggca accacatccg cagtctcaat tcgcaaggtg tggttaaaat
     9121 taaagacggg gaagtcacgc aggcagacat cgaccgcgcc attgaaacgg caaaggcaat
     9181 caatatcccg cccgatcaaa aaattctcga tgccgtggtt caagactaca ttatcgacac
     9241 ccaacttggc gtgagggagc ccatcggtat gagcggtgtg cgtctggata cgcgggtgca
     9301 catcattacc ggtgcaagta cggcagtgca gaatgtccaa aaatgtatcg agcggtgtgg
     9361 tttgaaaagc gatcagatta tgcttcagcc gttggcaagc gggcaggcgg tgctgactga
     9421 agatgaaaaa gacctcggcg tatgcgtcat cgacattggt ggcggaacga ccgatattgc
     9481 cgtttatatg aacggtgcca tccgccatac gtccgtcatt ccggccggtg gtaatctgat
     9541 taccaaagat ttgtccaaat cgttgagaac acctctcgat gccgccgagt acattaaaat
     9601 ccattatggc gtggcatcat gcgatacgga aggcttgggt gagatgattg aagttccggg
     9661 cgtgggtgac cggacatcgc gtcaggtttc cagtaaggtt ttggcagcaa tcatcagcgc
     9721 acgtattcag gagatttttg gcgtagtgct gggcgagctg caaaaatcgg gtttccccaa
     9781 agaagtgctg aatgcgggta tcgttctgac cggcggtgtg tccatgatga ccgggattgt
     9841 ggaatttgcc gaaaaaatct tcgatttgcc tgtacgcacc ggtgcacccc aagaaatggg
     9901 cggtttgtcc gaccgcgtcc gcacaccgcg tttttctacc gctatcgggc tgcttcatgc
     9961 agcatgcaag ctggaaggaa acttgccgca gccggaaaac ggtgcagtgc aagagaggga
    10021 agtgggcggc ggtttgttgg caagattgaa acggtggatt gaaaacagct tctgaacagg
    10081 tggattgccg tttgacaggt gagaagtatt ttgccagcag caagatactt cttatataat
    10141 gaataataat ttatttaaac cgtcctctga atggggcgag caggagtttt tgaatggaat
    10201 ttgtttacga cgtggcagaa tcggcagtca gccctgcggt gattaaagta atcggcttgg
    10261 gcggcggcgg ttgcaatgca atcaataaca tggttgccaa caatgtgcgc ggtgtggagt
    10321 ttatcagtgc caatacggat gcgcagtctc tggcaaaaaa ccatgcggcg aagagaatcc
    10381 agttgggtac gaatctgaca cgcggtttgg gcgcgggcgc gaatcccgat atcggccgtg
    10441 cggcagccca ggaagaccgg gaagccattg aagaagccat tcgcggtgcg aatatgctgt
    10501 ttatcacgac cggtatgggc ggcggtaccg gtaccggttc cgcgccggtt gttgctgaga
    10561 ttgccaagtc tttgggcatt ctgaccgttg ccgtggttac ccgaccgttc gcatatgaag
    10621 gtaagcgcgt ccatgtcgca caggcagggt tggaacagtt gaaagaacac gtcgattcgc
    10681 tgatcatcat cccgaacgac aaactgatga ctgcattggg tgaagacgta acgatgcgcg
    10741 aagccttccg tgccgccgac aatgtattgc gcgatgcggt cgcaggcatt tccgaagtgg
    10801 taacttgccc gagcgaaatc atcaacctcg actttgccga cgtgaaaacc gtgatgagca
    10861 accgcggtat cgctatgatg ggttcgggtt atgcccaagg tatcgaccgt gcgcgtatgg
    10921 cgaccgacca ggccatttcc agtccgctgc tggacgatgt aaccttggac ggagcgcgcg
    10981 gtgtgctggt caatattacg accgcacccg gttgcctgaa aatgtccgag ttgtctgaag
    11041 tcatgaaaat cgtcaaccaa agcgcacatc ccgatttgga atgcaaattc ggtgcggctg
    11101 aagacgagac catgagcgaa gatgccatcc ggattaccat tatcgctacc ggtctgaaag
    11161 aaaaaggcgc ggtcggtttt gttccggcaa gggaggtaga agcggttgcc ccgtccaaac
    11221 aggagcaaag ccacaatgtc gaaggtatga tccgcaccaa tcgcggtatc cgcacgatga
    11281 accttaccgc tgcggatttc gacaatcagt ccgtacttga cgactttgaa atccctgcga
    11341 ttttgcgtcg tcaacacaat tcagacaaat aatgtgctgt ttgcccgtaa acctgctgcc
    11401 tcccgaatcg gtttgtccgg tttgggaggt atgtttttca agatgttgca atttcgtacg
    11461 gtttgcggtc ggcggattca gatttttcca cttgatacag actttcagat atggacactt
    11521 caaaacaaac actgttggac gggattttta agctgaaggc aaacggtacg acggtgcgta
    11581 ccgagttgat ggcgggtttg acaacttttt tgacgatgtg ctacatcgtt atcgtcaacc
    11641 ctctgatttt gggcgagacc ggcatggata tgggggcggt attcgtcgct acctgtatcg
    11701 cgtctgccat cggctgtttt gttatgggtt ttgtcggcaa ctatccgatt gcactcgcac
    11761 cggggatggg gctgaatgcc tatttcacct ttgccgtcgt taagggtatg ggcgtgcctt
    11821 ggcaggttgc gttgggtgcg gtgttcatct ccggtctgat tttcatcctg ttcagctttt
    11881 ttaaagtcag ggaaatgctg gtcaacgcac tgcctatggg tttgaaaatg tcgattgctg
    11941 ccggtatcgg tttgtttttg gcactgattt ccctgaaagg cgcaggcatt atcgttgcca
    12001 atccggcaac cttggtcggt ttgggcgata ttcatcagcc gtccgcgttg ttggcactgt
    12061 tcggttttgc tatggtggtc gtattgggac atttccgcgt tcaaggcgca atcatcatca
    12121 ccatcttgac cattaccgtc attgccagcc tgatgggttt gaatgaattt cacggcatca
    12181 tcggcgaagt accgagcatt gcgccgactt ttatgcagat ggattttgaa ggcctgttta
    12241 ccgtcagcat ggtcagtgtg attttcgtct tcttcttggt cgatctattt gacagtaccg
    12301 gaacgctggt cggcatatcc caccgtgccg ggctgctggt ggacggtaag ctgccccgcc
    12361 tgaaacgcgc actgcttgca gactctaccg ccattgtggc aggtgcggct ttgggtactt
    12421 cttccaccac gccttatgtg gaaagcgcgg cgggcgtatc ggcaggcgga cggaccggcc
    12481 tgacggcggt taccgtcggc gtattgatgc tcgcctgcct gatgttttca cctttggcga
    12541 aaagtgttcc cgcttttgcc accgcgcccg ccctgcttta tgtcggcacg cagatgctcc
    12601 gcagtgcgag ggatattgat tgggacgata tgacggaagc cgcacccgca ttcctgacca
    12661 ttgtcttcat gccgtttacc tattcgattg cagacggcat cgccttcggc ttcatcagct
    12721 atgccgtggt taaactttta tgccgccgca ccaaagacgt tccgcctatg gtatggattg
    12781 ttgccgtatt gtgggcactg aaattctggt atttgggctg attgattcga tattaaaaat
    12841 gccgtctgaa aggttttcag acggcatttt gtttgccgat atatttaatt tttattaaat
    12901 tatataaaaa tcaaatacat aataaaatac atcggattgc ttaaaaataa tacattgttt
    12961 ttatgtataa aatattttat aagttttcag gattttgatt atcgaaaatt tttcttgatt
    13021 tcctgacaat tttattgaaa caaataattc aaaattaatc tagtttaatc atagaattaa
    13081 aataaaatat taaaattatg taatgagtct ccttaaaaat gtttgacatt ttcagtcttg
    13141 tgttttagat tatcgaaaaa taaaactaca taacactaca aaggaacatt actatgaaac
    13201 caattcagat gttttcccct tttctgaata atccccttgt tttcttcttg tctgcggttt
    13261 tgccgcataa ttccgaacgg tctgctgttt ttctttgatt cgttttaaat atcaataaga
    13321 taatttttcc catatatttt taatgattgg attgggatgc ccgatgcgtc ggatggatgt
    13381 gttttgccgt ccgaatgtga tggaagcctg tccatactga aaaaagtcta taaaggagaa
    13441 atatgatgag tcaacactct gccggagcac gtttccgcca agccgtgaaa gaatcgaatc
    13501 cgcttgccgt cgccggttgc gtcaatgctt attttgcacg attggccacc caaagcggtt
    13561 tcaaagccat ctatctgtcc ggcggcggcg tggcagcctg ttcttgcggt atccctgatt
    13621 tgggcattac cacaatggaa gatgtgctga tcgacgcacg acgcattacg gacaacgtgg
    13681 atacgcctct gctggtggac atcgatgtgg gttggggcgg tgcattcaat attgcccgta
    13741 ccattcgcaa ctttgaacgc gccggtgttg cagcggttca catcgaagat caggtagcgc
    13801 aaaaacgctg cggccaccgt ccgaacaaag ccattgtatc taaagatgaa atggtcgacc
    13861 gtatcaaagc tgccgtagat gcgcgcgttg atgagaactt cgtgattatg gcgcgtaccg
    13921 atgcgctggc ggtagaaggt ttggatgccg ctatcgaacg cgcccaagct tgtgtcgaag
    13981 ccggtgcgga catgattttc cctgaagcca tgaccgattt gaacatgtac cgccaatttg
    14041 cagatgcggt gaaagtgccc gtgttggcga acattaccga gtttggttcc actccgcttt
    14101 atacccaaag cgagctggct gaaaacggcg tgtcgctggt gctgtatccg ctgtcatcgt
    14161 tccgtgcagc aagcaaagcc gctctgaatg tttacgaagc gattatgcgc gatggcactc
    14221 aggcggcggt ggtggacagt atgcaaaccc gtgccgagct gtacgagcat ctgaactatc
    14281 atgccttcga gcaaaaactg gataaattgt ttcaaaaatg atttaccgct ttcagacggt
    14341 ctttcaacaa atccgcatcg gtcgtctgaa aacccgaaac ccataaaaac acaaaggaga
    14401 aataccatga ctgaaactac tcaaaccccg actttcaaac ctaaaaaatc cgttgcgctt
    14461 tcaggcgttg cggccggtaa taccgctttg tgtaccgttg gccgtaccgg caacgatttg
    14521 agctatcgcg gttacgacat cttggatttg gcacaaaaat gcgagtttga agaagtcgcc
    14581 cacctgctga ttcacggtca tctgcccaac aaattcgagc tggccgctta taaaaccaag
    14641 ctcaaatcca tgcgcggcct gcctatccgt gtgattaaag ttttggaaag cctgcctgca
    14701 catacccatc cgatggacgt aatgcgtacc ggcgtatcca tgctgggctg cgttcatcct
    14761 gaacgtgaaa gccatccgga aagtgaagcg cgcgacatcg ccgacaaact gatcgccagc
    14821 ctcggcagca tcctcttgta ctggtatcaa tattcgcaca acggcaaacg cattgaggtt
    14881 gaaagcgacg aagagaccat cggcggtcat ttcctgcaac tgttgcacgg caaacgccca
    14941 agcgaatcac acatcaaagc catgcacgtt tcactgattc tgtatgccga acacgagttc
    15001 aacgcttcta cctttaccgc ccgcgtgatc gccggtacag gctctgatat gtactccagc
    15061 attaccggag caatcggcgc gttgaaaggt ccgaaacacg gcggcgcgaa cgaagtggct
    15121 tacgatattc aaaaacgcta ccgcaatgcc gacgaagctg aagccgacat ccgcgaacgc
    15181 atcggccgca aagaaatcgt gatcggtttc ggtcatccgg tgtacaccat ttccgaccct
    15241 cgcaacgttg tcattaaaga agtggcacgc ggtttgagca aagaaaccgg cgatatgcgc
    15301 ctctttgaca ttgccgaacg tttggaaagc gtgatgtggg aagagaaaaa aatgttcccg
    15361 aatctggact ggttctctgc cgtttcctac caaaaattgg gcgtaccgac cgctatgttc
    15421 acaccgctgt tcgtaatttc ccgtacaacc ggttggagcg cacacgttct tgagcaacgc
    15481 aaagacggca aaatcatccg tccgagcgca aactacacag gccctgaaga tttggcgttt
    15541 gtggagattg aagaacgctg agacggaaca caaccgtagg agaccaccaa tcggatttca
    15601 gacgacgtgt agcgcgggct ttgcctgcgg aaaactgaaa cccgcctacg gttccgcttt
    15661 tcagacggcc tgttgaaaga tgcgagagag aaggttgtct gaaaaaccag ccgcaacgtc
    15721 tgccgtcatt cccacccccg cctaagcgag gacaggtcgc ggcgggaatg gcggaggcag
    15781 cgcggcaact tttttcacgg ttctgccaaa atatgctatg aaagaatgtt cgtcagcata
    15841 atggagatga agccgatatt ataactaata cggagaattg tgggatatgt accataagaa
    15901 atttgataaa gaaattcttt taaaaattaa agaattatat agatacgata attggcatgc
    15961 tgtacttgct atattatatg attatttaat aataattttt tctatatttt tatctgagca
    16021 gtcatattgg tttctccctt taacgatttt attaatagga agcaggcaaa gggcgttggc
    16081 aacaatttta catgaagcct ctcattctgc cttaactaaa aataagaagt ttggacaaat
    16141 tttaggtaca tatttttctg gatatttaat ttttcaaagt tggaatagtt attgtgtttc
    16201 tcatgtgaag aatcatcatc caaagttagg gactgattcg gatcctgatt ataagtatta
    16261 taaagactca ggtatttttc agacatattc aagaattgaa tttttctgga gattttgtgt
    16321 ctctcaaatt ttatgtttga aattatttag gaattttaaa tatttaatta aaaatagatt
    16381 attttcggat attacaagaa cagagttgtt taagctactt ctttctcaat taattctatt
    16441 tgttatcggt acatattttg tagggtataa attttatgta atttattggt tattaccata
    16501 tttaactgca ttccagatga taacttggtt tattgagttg tccgagcatt atccaatgat
    16561 acaaactgcc aaaaataatt tagaagcgtc aagaaatagg tttagtcatg caatagagca
    16621 ttttttcacg gggatacatg gagaaaatta tcatctaata catcatttat ttccagccat
    16681 tccattttgg aatttggaaa aagcacataa gatattaatg aaagattttg attatcaaga
    16741 ggtaaataaa gattttggag ggattttctt ttcgaaaaac aataatgcac ctatgtggaa
    16801 agtattatgg agtaaatatg aagctgcgtg atttactgat gggaatattc ttggcagttt
    16861 ctgcggccct tctgaatgca accatcggca tattcagcaa gatattgatg gagcagggct
    16921 tgtctgttca gcatattgca tttttgaaaa ctttgacagg tttcgtgttt atcagcattt
    16981 tgctttgccg taccggtttt accagacaga ttgcggatat ttcaagaaag aaagaggcaa
    17041 ttttgccgtt gctgttaaaa gtagcaattt gtgctttttt cggaatttat acgttgtttt
    17101 tctttgaaac cacagcttat caatatggca atgctgcgaa tgtagtagtt gtattaatgg
    17161 catcggctgc cgtatctgcc ttgatattgg acagcatact gttagatgaa cgtatttgca
    17221 tttcttcagt cgtcggtgtg ggtttggcag tattggggat cgcaatgatt tcttggactg
    17281 gagaaggaag tttagggttg attctgaatg ccgcactggc gggctcgggc tacggttgtt
    17341 tttccgtttt gattaagaaa ttcggcctaa acggcggtat ttatttgaca cggatattga
    17401 tgttttttgg aagtattttt ttgtttatcc cttcattgga aggtattgag gatatacatt
    17461 ggcaatggtc ttttattccg ccactcttgg cattgtcttt attgccgacg attttaggat
    17521 tttattgtac aactaaagca ttggattatt tgagtgctgc gaaggtacag gtaactgaat
    17581 tggccgagcc attgtttgct gccgtactgg cttggttgtt tttgaatgaa ataccggaag
    17641 gacgcttctt tgtcggcgcc attctgatta ttgccggtat tgtgtctatc aatgggctgt
    17701 atcgaccatt gttgaagcga attgaataat ttttatttcc caagatttca gacgacctct
    17761 tcaacaaacg tcgtctgaaa ccccaaaacc aaaaaaataa tagatttagg agaaccgaca
    17821 tggctgccaa ccaacgttac cgcaaaccgc tgcccggtac ggatttggaa tactacgacg
    17881 cgcgtgcggc gtgtgaggac atcaagcccg gctcttacga caagctgcct tacacgagcc
    17941 gcattttggc ggagaatttg gtcaaccgcg cggacaaagt cgatttgccg acgctgcaaa
    18001 gctggctggg tcagctgatt gagggaaaac aggaaatcga ctttccttgg tatccggcgc
    18061 gggtggtgtg ccacgatatt ctggggcaga ccgcgttggt ggatttggca ggtctgcgcg
    18121 atgcgattgc cgaaaaaggc ggcgatcctg ccaaagtgaa tccggtggtg caaacccagc
    18181 tcatcgtcga ccactctctg gccgttgaat gcggcggcta cgaccccgat gccttccgca
    18241 aaaaccgcca aatcgaagac agacgtaacg aagaccgttt ccacttcatc aactggacaa
    18301 aaaccgcatt tgaaaatgtg gacgtgattc cggcgggcaa cggcatcatg caccaaatca
    18361 atctagaaaa aatgtcgccc gtcgtccaag tcaaaaacgg cgtggcgttc cccgatacct
    18421 gcgtcggcac ggattcgcac acgccgcacg tcgatgcgct gggcgtgatt tccgtgggcg
    18481 tgggcggatt ggaagcggaa accgtgatgc tgggtcgcgc gtccatgatg cgcctgcccg
    18541 atattgtcgg cgttgagctg aacggcaaac ggcaggcggg cattacggcg acggatattg
    18601 tgttggcact gaccgagttt ctgcgcaaag aacgcgtggt cggggcgttt gtcgaattct
    18661 tcggcgaggg cgcgagaagc ctgtctatcg gcgaccgcgc gaccatttcc aacatgacgc
    18721 cggagttcgg cgcgactgcc gcgatgttcg ctattgatga gcaaaccatt gattatttga
    18781 aactgaccgg acgcgacgac gcgcaggtga aattggtgga aacctacgcc aaaaccgcag
    18841 gcttatgggc agatgccttg aaaaccgccg tttatccgcg cgttttgaaa tttgatttga
    18901 gcagcgtaac gcgcaatatg gcaggcccga gcaacccgca cgcgcgtttt gcgaccgccg
    18961 atttggccag caaaggcttg gctaaacctt acgaagagcc ttcagacggc caaatgcccg
    19021 acggcgcggt catcatcgcc gcgattacca gttgcaccaa cacttccaac ccgcgcaacg
    19081 ttgttgccgc cgcgctcttg gcgcgcaacg ccaactgctt cgggctgaaa cgcaaaccgt
    19141 gggtcaaaac ctcgtttgcc cccggttcga aagtggcgga aatttatttg aaagaagcag
    19201 gcctgctgcc cgaaatggaa aaactcggct tcggtatcgt cgccttcgcc tgcaccacct
    19261 gcaacggcat gagtggcgcg ctggatccga aaatccagaa agaaatcatc gaccgcgatt
    19321 tgtacgccac cgccgtatta tcaggcaacc gcaacttcga cggccgtgtc catccgtatg
    19381 cgaaacaggc tttcctcgct tcgcctccgt tggtcgttgc ctacgcgctg gcaggcagta
    19441 tccgtttcga tattgaaaac gacgtactcg gcgttgcaga cggcaaggaa atccgcctga
    19501 aagacatttg gcctgccgat gaagaaatcg atgccgtcgt tgccgaatat gtgaaaccgc
    19561 agcagttccg cgatgtgtat gtaccgatgt tcgacaccgg cacagcgcaa aaagcaccta
    19621 gtccgctgta cgattggcgt ccgatgtcca cctacatccg ccgtccgcct tactgggaag
    19681 gcgcgctggc aggggaacgc acattaagag gtatgcgtcc gctggcgatt ttgcccgaca
    19741 acatcaccac cgaccacctc tcgccgtcca atgcgatttt ggccgtcagt gccgcaggcg
    19801 agtatttggc gaaaatgggt ttgcctgaag aagacttcaa ctcttacgca acccaccgcg
    19861 gcgaccactt gaccgcccaa cgcgctacct tcgccaatcc gaaactgttt aacgaaatgg
    19921 tgaaaaacga agacggcagc gtgcgccaag gctcgttcgc ccgcgtcgaa cccgaaggcg
    19981 aaaccatgcg catgtgggaa gccatcgaaa cctatatgaa ccgcaaacag ccgctcatca
    20041 tcattgccgg tgcggactat ggtcaaggct caagccgcga ctgggctgca aaaggcgtac
    20101 gcctcgccgg cgtagaagcg attgttgccg aaggcttcga gcgtatccac cgcaccaacc
    20161 ttatcggcat gggcgtgttg ccgctgcagt tcaaacccga caccaaccgc cataccctgc
    20221 aactggacgg tacggaaacc tacgacgtgg tcggcgaacg cacaccgcgc tgcgacctga
    20281 ccctcgtgat tcaccgtaaa aacggcgaaa ccgtcgaagt tcccgttacc tgccgcctcg
    20341 atactgcaga agaagtattg gtatatgaag ccggcggcgt gttgcaacgg tttgcacagg
    20401 attttttgga agggaacgcg gcttagaggt cgtctgaaaa gcaagacgta gcgtgggtcg
    20461 ggttcaacat tttgctcatt cacgtaattc tcgatatggc aggcatctac tgtaaatcgt
    20521 cattcccgcg caggcgggaa tccagaaagt ggaattgagg aaaccttttt atccgatgag
    20581 tttctgtgcg gataaatctg gattcccgcc tgcgcgggaa tgacggggtt taataatctg
    20641 ccgtatcaca acacagtagc cgtagattgt ggcgaacccc gacagtttgc ggaatcaaac
    20701 ggctttggtc ggagtggcag cctaatccac tataaaaatc gtgggcagag cccacgctac
    20761 ataaggagaa tctagaaatg ccgcaaatta aaattcccgc cgtttactac cgtggcggta
    20821 catcaaaagg cgtgtttttc aaacgttccg acctgcccga ggcggcgcgg gaagcgggaa
    20881 gcgcacgcga caaaatcctc ttgcgcgtac tcggcagccc ggatccctac ggcaagcaga
    20941 tagacggttt gggcaacgcc agctcgtcca ccagcaaggc ggtgattttg gacaagtccg
    21001 aacgcgccga tcacgatgtc gattaccttt tcgggcaagt ttccatcgac aaaccttttg
    21061 tcgattggag tggcaactgc ggcaacctca ccgccgccgt gggcgcattt gccatcgagc
    21121 aaggcttggt cgataaaggc aagattcctt cagacggcat ctgcacagtc aaaatctggc
    21181 agaaaaacat cggcaaaacc attattgccc atgtaccgat gcaaaacggc gcagttttgg
    21241 aaacaggcga ttttgagctc gacggcgtaa cgttcccggc agccgaagta caaatcgaat
    21301 ttcttgatcc agccgacggc gaaggcagta tgttcccaac cggcaatttg gtcgatgaaa
    21361 ttgatgtgcc gaatataggc cgtttgaaag ccacgctcat caacgcgggc attccgaccg
    21421 ttttcctgaa tgccgccgac ttgggctaca cgggcaaaga gttgcaagac gacatcaaca
    21481 acgatgccgc ggctttggaa aaattcgaga aaatccgcgc ttacggtgcg ctgaaaatgg
    21541 gtctgatcag cgacgtatcc gaagctgccg cccgcgcgca cacgccgaaa gtcgccttcg
    21601 tcgcgcccgc cgccgattac accgcctcca gtggcaaaac cgtgaacgcc gccgacatcg
    21661 atttgctggt acgagccctg agcatgggca aactgcacca cgcgatgatg ggtaccgcct
    21721 ctgttgccat tgcgaccgcc gccgccgtgc ccggtacgct ggtcaacctt gccgcaggcg
    21781 gcggaacgcg taaagaagtg cgcttcgggc atccttccgg cacattgcgc gtcggtgcag
    21841 ccgccgaatg tcaggacgga caatggacgg ccaccaaagc ggtcatgagc cgtagcgcac
    21901 gcgtgatgat ggaaggttgg gtcagggtgc ctgaggattg tttttaaatt gacgtagcat
    21961 gggtttgccc gcgagccata aacaggtcgt ctgaaaaaca agtaaacatc aaatcactga
    22021 ccattccttt cccttgccct gtggcggaag gcggcaaatc acaaggaaga acacggaaac
    22081 cccgataaaa gacagcttcc cgtattaccg tcattcccgc gcaggcggga atctagacct
    22141 gtcaatatgg aggattggta ggggaaaaca ggtttcgtga gttctacatt ctggattccc
    22201 gccacagcct gtcctcgcgt aggcggggac ggaataacga tagaaaatgc ggcatacgct
    22261 ttgcccaaag aggccgtctg aaacaccttg cgcctgatgt ctgccttttt cagacgaccc
    22321 cacaccaaaa aaacaaccac aaactacaag gagaaacatc atgtccgacc aactcatcct
    22381 cgttctgaac tgcggcagtt catcgctcaa aggcgccgtt atcgaccgaa aaagcggcag
    22441 cgtcgtccta agctgcctcg gcgaacgcct gaccacgccc gaagccgtca ttacgttcaa
    22501 caaagacggc aacaaacgcc aagttcccct gagcggccga aattgccacg ccggcgcggt
    22561 gggtatgctt ttgaacgaac tggaaaaaca cggtctgcac gaccgcatca aagccatcgg
    22621 ccaccgcatc gcccacggcg gcgaaaaata cagcgagtct gttttgatcg accaggccgt
    22681 aatggacgaa ctcaatgcct gcattccgct tgcgccgctg cacaaccccg ccaacatcag
    22741 cggcatcctt gccgcacagg aacatttccc cggtctgccc aatgtcggcg tgatggatac
    22801 ttcgttccac caaaccatgc cggagcgtgc ctacacttat gccgtgccgc gcgagttgcg
    22861 taaaaaatac gctttccgcc gctacggttt ccacggcacc agtatgcgtt acgttgcccc
    22921 tgaagccgca cgcatcttgg gcaaaccttt ggaagacatc cgcatgatta ttgcccactt
    22981 aggcaacggc gcatccatta ccgccatcaa aaacggcaaa tccgtcgata ccagtatggg
    23041 tttcacgccg atcgaaggtt tggtaatggg tacacgttgc ggcgacatcg atccgggcgt
    23101 atacagctat ctgacttccc acgccgggat ggatgttgcc caagtggatg aaatgctgaa
    23161 caaaaaatca ggtttgctcg gtatttccga actttccaac gactgccgca ccctcgaaat
    23221 cgccgccgac gaaggccacg aaggcgcgcg cctcgccctc gaagtcatga cctaccgcct
    23281 cgccaaatac atcgcttcga tggctgtggg ctgcggcggc gttgacgcac tcgtgttcac
    23341 cggcggtatc ggcgaaaact cgcgtaatat ccgtgccaaa accgtttcct atcttgattt
    23401 cttgggtctg cacatcgaca ccaaagccaa tatggaaaaa cgctacggca attcgggcat
    23461 tatcagcccg accgattctt ctccggctgt tttggttgtc ccgaccaatg aagaactgat
    23521 gattgcctgc gacactgccg aacttgccgg catcttgtag ccaaaaaagg gacgagtccg
    23581 caaaaatgcc gtctgaaatt ttcagacggc attttgtttg gattttgggc gtacaatacg
    23641 gaaaacatga cgataaggac acaaactatg gcacagtttt tcgctattca tcccgacaat
    23701 ccccaagaac gcctcatcaa gcaggcggtt gaaatcgtca ataaaggcgg cgtggtcgtc
    23761 tatccgaccg attcgtgtta cgccttgggt tgcaaactcg gcgataaggc ggcgatggaa
    23821 cgcatactct ccatccgcaa aatcgatttg aaacaccacc tgaccctgat gtgcgccgat
    23881 ttgagcgagt tgggcacgta cgccaaaatc gacaacgtac agtttcgtca gcttaaggcc
    23941 gccacgcccg gaccttatac ctttatcttg caggcgacga aggatgtgcc ggcgcgcacg
    24001 ctgcacccga aacgcaaaac catcgggctg cgtattcccg ataatgccat tgcacaagcc
    24061 ctgctggggg aattgggcga gccgctttta agctgcaccc tgatgctgcc cgaagacggc
    24121 gaaccattga ccgatcctta tgaaatccgc gagcgtttgg aacacgccgt cgatttggtg
    24181 attgacggcg gctggtgcgg aaccgagccg accaccgtcg tcgatatgac cgacggcacg
    24241 gaattggtgc gccaaggttg cggcgatacg gcggtgttcg gtttgtaggg aaaccgatgc
    24301 cgtctgaagc atcggctgtt cagacggcat tgcgcgcctt gccggcggca gtccgaaatg
    24361 ccggcgcgta tcgcgctcgg tcggaatatc cgtttgaaac ggcattttga tgcattactg
    24421 caccgcaatc ggaattctcg gttcgtagag caggtcgtag gtcggcttgt tgagcaggtc
    24481 ttggagcgtg aaaccgtcca gatacgtgaa aaacgacttc atcgcgccgc cgagtatgcc
    24541 cgtcagccgg caggacggtg taatcaggca ttcgttgttc tcgcccatgc actcgaccag
    24601 ctgcatcggt tcgaggtggc ggacaaccga gccgatgttg atgcggtcgg gcggtgcggc
    24661 aagccgcaga ccgccgcctt ttccgcgcac actgtggagg aagccgcctt tgaccagcgc
    24721 ggtaacgacc ttcatcagat ggcttttgga aatgccgtag gttacggcga tggtactgat
    24781 gttgaccagc gcatcgtcgt tgatggcagt gtagataagg acgcgcagcc cgtagtccgt
    24841 atgttgtgtc aaatacatga ttttctcggt atggattgtt attcttatcg gtacggttta
    24901 aggttcacgg acaatacctt aatggttgaa accctgtccg tcggggcggt agaatgcagc
    24961 ctgtctgcgg cggtatgccg tctgaaacat ccgcgctacc gtttgataat ttgttattgt
    25021 aactcaaaat catgaaaccg ttgaaacgac atcccgccct tatcgggctt tcgcgtgacc
    25081 accaccattc gctttccctg tgcgtgcgtc tgttgcggac gccggaagaa aggcatcggg
    25141 acgaactcga accgcatttt gccgaattgg aaacccattt tcgcgaagaa gaagccaagt
    25201 ttgccccaat ttggcagaat gtcgcccccg aattgaaaca acgtttcgag aaagaccacg
    25261 cccgactgcg gcagatgatg gcaagccccg aatacggtaa cgcggcgtgg aataccgctt
    25321 ttgccacaac cctgcgcgac cacgcgcgct ttgaagaacg cgagctgttt cccgccgccg
    25381 aaccgttttt gccggcatga ttccgttttg cggcaaatat attaatgata aacaaggaac
    25441 acacatgaaa tttaccaagc accccgtctg ggcaatggcg ttccgcccat tttattcgct
    25501 ggcggctctg tacggcgcat tgtccgtatt gctgtggggt ttcggctaca cgggaacgca
    25561 cgagctgtcc ggtttctatt ggcacgcgca tgagatgatt tggggttatg ccggactggt
    25621 cgtcgtcgcc ttcctgctga ccgccgtcgc cacttggacg gggcagccgc ccacgcgggg
    25681 cggcgttctg gtcggcttga ctatcttttg gctggctgcg cggattgccg cctttatccc
    25741 gggttggggt gcgtcggcaa gcggcatact cggtacgctg tttttctggt acggcgcggt
    25801 gtgcatggct ttgcccgtta tccgttcaca gaatcaacgc aactatgttg ccgtgttcgc
    25861 gctgttcgtc ttgggcggca cgcatgcggc gttccacgtc cagctgcaca acggcaacct
    25921 aggcggactc ttgagcggat tacagtcggg cttggtgatg gtgtcgggtt ttatcggtct
    25981 gattggtacg cggattattt cgttttttac gtccaaacgg ttgaatgtgc cgcagattcc
    26041 cagtccgaaa tgggtggcgc aggcttcgct gtggctgccc atgctgaccg ccatgctgat
    26101 ggcgcacggc gtgatgcctt ggctgtcggc ggctttcgcg tttgcggcag gcgtgatctt
    26161 taccgtgcag gtgtaccgct ggtggtataa acccgtgttg aaagagccga tgctgtggat
    26221 tctgtttgcc ggctatctgt ttaccggatt ggggctgatt gcggtcggcg cgtcttattt
    26281 caaacccgct ttcctcaatc tgggtgtgca tctgatcggg gtcggcggta tcggcgtgct
    26341 gactttgggc atgatggcgc gtaccgcgct tggtcatacg ggcaatccga tttatccgcc
    26401 gcccaaagcc gttcctgtcg cattttggct gatgatggcg gcaactgccg tccgcatggt
    26461 tgccgtattt tcttccggta cagcctacac gcacagcatc cgcacctctt cggttttgtt
    26521 tgcacttgcg cttttggtgt atgcgtggaa atacattccg tggctgattc gtccgcgttc
    26581 ggacggcagg cccggttgag acaaaccgcc gcagatttcg ggtctgggcg gtttgctttt
    26641 cagacggcag ggcggtcagt tgccgtccag ccagcggtcg cgtgtggttt tggcttcttc
    26701 aaaatagcgg tacagggctt cgcggtcgtc ggtggtcagg atgtttgcca aaacgtccaa
    26761 ctgtttgccc aagccttgaa ccagttgcag caggctgtct ttgttggcaa ggcagatgtc
    26821 cgcccacacg gcgggatggc cggaggcgat gcgggtgaag tcccgaaagc ccgtggcggc
    26881 gaatttcaga tattcctgtc cgtcggggtg gtcgagaatc tggtggacat aggcgaaggc
    26941 ggtcaggtgg ggcatatggg agacggcggc gaaaaccgcg tcgtggcgtt gcgcgtccat
    27001 cgtataaatt tccgcaccga ccgcgtgcca cagttcttct accaaggcaa tgccgtctga
    27061 atgttcgccg ccgtgtggcg tgatgatgag ttttctgtgg cggaacagcc cgaactgcgc
    27121 ggcttgcgca ccgcttctgt ccgaaccggc aatcgggtgg gcggcgatgc agcggtgcag
    27181 gcggtcgggc agacagcggc ggaaggcttc gatgaccgaa gatttggtgc tgccgacatc
    27241 ggaaatccaa gtgtgttccg gcaaaacggg gcgcagcgcg gtcaaaatgg cgggaaaggt
    27301 ggcgacgggc gtggcaatca gtaccaagtc cgcaccgccg atgctgtccg cgtcgatggc
    27361 aacggaagcc cggtcaatca cgccgcgttc caatgcacgt tcgaggttgt cgcggtcggt
    27421 gtcgataccg gtaacggtgc ggacgagtcc ctgccttttg aggtcgagaa cgaacgaacc
    27481 gccgatcagc cctacaccga tgagggcaat atggttcaaa atgggcattt gtgtaaacgg
    27541 ttttcgcaaa gtaccgtcat ggtagcctat cggcggaata tgccgcaagg tcggcaggaa
    27601 aaaggagaag aaatggacaa aatcagagtt gtcgccgtgc agatggtgtc gggcgtgtcg
    27661 ccggaaacca acgtcgccgc catgaaacgc ctggtcgcac gggcagcgga gcagggtgcg
    27721 gattgggtgc tactgcccga atattgggtg ctgatgggcg caaacgatac cgacaaactc
    27781 gcgcttgccg agcctttggg cggcggacgc tttcagacgg cattgagcga aacggcgaaa
    27841 gaatgcggcg tggtgctgtt tggcggaacc gtgccgctgc aaagccccga ggcgggcaag
    27901 gtcatgaata ctttattggt gtacgggcgc gacggcaaac ggacggggct gtaccacaaa
    27961 atgcacctct tcggcttttc cggtttgggc gaacgctatg ccgaagccga taccatccgc
    28021 gcgggcgggg aagtgccgca cttgtcggca gaaggcgtgt cggtggcggc gggcatttgt
    28081 tacgatgtcc gctttcccga atttttccgc cgccagctgc cgtttgacgt attgatgctg
    28141 cccgctgcgt ttacgcacac gacgggcaag gcgcattggg agctgctgct gcgcgcgcgt
    28201 gccgtcgaaa accaatgtta cgtcgtggcg gcggcacagg gcggtttgca cgaaaacgga
    28261 cggcgcacgt tcggacacag catgattgtc gatccgtggg gcgacgtgtt ggacgtattg
    28321 cccgaaggcg aaggcgttgt tacggcagac atcgatgcca atcgcctgaa cagcgtccgc
    28381 aaccgcctgc ccgccttgaa ataccgggtt ttggatgccg tctgaaggtt cagacggcat
    28441 cggtgccggg gaatcagcgg gctgtaggat gggcttcagc ctgccatttc catcaatccc
    28501 acatttctac cgttttcatc gaatccatcg aatccgcccc tttcgacgac ccagtcc
//
LOCUS       DAXHRI010000008        27594 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXHRI010000008 DAXHRI010000000
VERSION     DAXHRI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27594
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.008"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(67..906)
                     /locus_tag="ACFM75_000251"
     CDS             complement(67..906)
                     /locus_tag="ACFM75_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC6254857.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     ATPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1191..1266
                     /locus_tag="ACFM75_000252"
     tRNA            1191..1266
                     /locus_tag="ACFM75_000252"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1224..1226,aa:Arg,seq:ccg)
     gene            complement(1331..2524)
                     /locus_tag="ACFM75_000253"
     CDS             complement(1331..2524)
                     /locus_tag="ACFM75_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC6254858.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     NRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2545..3633)
                     /gene="trmA"
                     /locus_tag="ACFM75_000254"
     CDS             complement(2545..3633)
                     /gene="trmA"
                     /locus_tag="ACFM75_000254"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC6254859.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3776..4876
                     /gene="aroC"
                     /locus_tag="ACFM75_000255"
     CDS             3776..4876
                     /gene="aroC"
                     /locus_tag="ACFM75_000255"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC6254860.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLALTESDIQAD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGCEHISQNPFFAANQSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4967..5392
                     /locus_tag="ACFM75_000256"
     CDS             4967..5392
                     /locus_tag="ACFM75_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC6254861.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5466..7451)
                     /gene="parE"
                     /locus_tag="ACFM75_000257"
     CDS             complement(5466..7451)
                     /gene="parE"
                     /locus_tag="ACFM75_000257"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC6254862.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDDHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7518..8066)
                     /locus_tag="ACFM75_000258"
     CDS             complement(7518..8066)
                     /locus_tag="ACFM75_000258"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC6254863.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINERNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQPSGNR"
     gene            8207..9502
                     /gene="serS"
                     /locus_tag="ACFM75_000259"
     CDS             8207..9502
                     /gene="serS"
                     /locus_tag="ACFM75_000259"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC6254864.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYEFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9575..10573)
                     /locus_tag="ACFM75_000260"
     CDS             complement(9575..10573)
                     /locus_tag="ACFM75_000260"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6254865.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGKYVDLDELYAQSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10698..11774)
                     /gene="prfA"
                     /locus_tag="ACFM75_000261"
     CDS             complement(10698..11774)
                     /gene="prfA"
                     /locus_tag="ACFM75_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC6254866.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATADMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDTDDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11864..12634)
                     /locus_tag="ACFM75_000262"
     CDS             complement(11864..12634)
                     /locus_tag="ACFM75_000262"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6254867.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILWIAEIAAQIRGVGVADIAAITERNADSLLRP"
     gene            complement(12749..13738)
                     /locus_tag="ACFM75_000263"
     CDS             complement(12749..13738)
                     /locus_tag="ACFM75_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC6254868.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     VKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGRLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13805..14488)
                     /locus_tag="ACFM75_000264"
     CDS             complement(13805..14488)
                     /locus_tag="ACFM75_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6254869.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14677..16011)
                     /gene="glmM"
                     /locus_tag="ACFM75_000265"
     CDS             complement(14677..16011)
                     /gene="glmM"
                     /locus_tag="ACFM75_000265"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC6254870.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTQPSARLGRARRINGADDRY
                     IEFCKSTFPTHSDLRGLKLVVDAANGAAYSVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRSWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16141..16992)
                     /gene="folP"
                     /locus_tag="ACFM75_000266"
     CDS             complement(16141..16992)
                     /gene="folP"
                     /locus_tag="ACFM75_000266"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC6254871.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGALELLACQADTGICLMHMRGLPENMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMIGELTGEADAAERVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(17132..18025)
                     /locus_tag="ACFM75_000267"
     CDS             complement(17132..18025)
                     /locus_tag="ACFM75_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC6254872.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18116..20230
                     /locus_tag="ACFM75_000268"
     CDS             18116..20230
                     /locus_tag="ACFM75_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC6254873.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRIFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTIGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLEAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20294..21772)
                     /gene="ubiD"
                     /locus_tag="ACFM75_000269"
     CDS             complement(20294..21772)
                     /gene="ubiD"
                     /locus_tag="ACFM75_000269"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC6254874.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVIGMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETEREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(21792..22307)
                     /locus_tag="ACFM75_000270"
     CDS             complement(21792..22307)
                     /locus_tag="ACFM75_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC6254875.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECGGDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22314..22748)
                     /locus_tag="ACFM75_000271"
     CDS             complement(22314..22748)
                     /locus_tag="ACFM75_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254876.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFGRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRRVTQVWCVFFIANGTL
                     AALLARLGRYDWWAVYTGVIAYVLMGMPFAGEWLYRKLVLKV"
     gene            complement(23029..23112)
                     /locus_tag="ACFM75_000272"
                     /pseudo
     CDS             complement(23029..23112)
                     /locus_tag="ACFM75_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            complement(23115..23615)
                     /locus_tag="ACFM75_000273"
                     /pseudo
     CDS             complement(23115..23615)
                     /locus_tag="ACFM75_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23671..24234
                     /locus_tag="ACFM75_000274"
                     /pseudo
     CDS             23671..24234
                     /locus_tag="ACFM75_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            24212..25348
                     /locus_tag="ACFM75_000275"
     CDS             24212..25348
                     /locus_tag="ACFM75_000275"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC6254877.1"
                     /translation="MDWHERFPELEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVD
                     AAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGMSRNFSANTYVR
                     MMPHTAAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFF
                     PSEVYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAII
                     YAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHADLAPEDIGWINLHGTGTHHND
                     SMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRKSNPEGKLPPQLW
                     DGQNDPDLPAINLTGSGSRWETEKRIAASSSFAFGGSNCVLLIG"
     gene            25402..25477
                     /locus_tag="ACFM75_000276"
     tRNA            25402..25477
                     /locus_tag="ACFM75_000276"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25435..25437,aa:Ile,seq:cat)
     gene            25552..26310
                     /locus_tag="ACFM75_000277"
     CDS             25552..26310
                     /locus_tag="ACFM75_000277"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6254878.1"
                     /translation="MKKVSVLIVARNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AESLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            26311..27375
                     /locus_tag="ACFM75_000278"
     CDS             26311..27375
                     /locus_tag="ACFM75_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6254879.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRDCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
BASE COUNT         6393 a         7795 c         7171 g         6235 t
ORIGIN      
        1 attcccataa aaaaacggag cagatacctg ccccgttttt atttaatccg aaattttaat
       61 ctaaatttag aattttgcac cggattggtt tgccatatag tcaacagccg ctttgacttc
      121 gtcatcgctc aaaccggcat tgccgccttt ggccggcatc gcgttaaagc cttcaagggc
      181 gtgtttgtgc aaggtttctt tgcctttttt gatacgcggt gcccaatcgt cttttttgcc
      241 tatgccggga ataccgggaa tcgaaccgcc gtggcacacc tgacaggttg cttcgaagac
      301 ttttttaccg tcaacgccga ccgcaggggc tgccgcaccc ttgtcttctg ccttcgcttc
      361 tgccggagct gcactatcgg caggagcaga agctgttcct gaagcggcat tgtcggcagg
      421 cgtagcctca tcaggattcg ggaaagaacc gccgcttttg ttcgccatgt aagtaatcgc
      481 ccgtttgagt tcctgatcgg tcaggtctgc cgcaccgcct ttggcaggca tggcgttaaa
      541 gccgttcagc gcgtgttgga acaaggtatc gaagccttgc gcgatacgcg gtgcccaatc
      601 gccgttgtgt tccagtttcg gagcgttcgg cacattgctg tccgccgcgt ggcattggat
      661 acagattttg ccgaaaatct gttcgccttg gcgttcgccg acggggatgc cgtcgcccat
      721 cgtcaattgt ccgacgggct ggatacgggt ctgcgttgcc gcttccgtag tggcatcgac
      781 atcgccgaac gagccgctgc ccgccagctt aatcaggaaa taaaggactg caataacaat
      841 aacgataccg ctcacaaggg taaacagtgc agagccttgg gctttattgt cgcggagttg
      901 tttcatttgg taggcctcgc cgtcaggtta ggttgtgctg taaattatag tttggtgtgt
      961 taaacgcagt taacaatatt ttgctggatt atactgaatt cacagggtct ttccaatcgc
     1021 tatcattgaa aatatgaaaa aatttgccaa cggtatctgt ataaaacaaa caatcctttg
     1081 aaaataatgg tttatcctca agaaaactct ccttatgccg ccatacgccg cctgccggcg
     1141 caagataacc tttgccaatt tgcagaattt acgttaacct tgcgttttcc gcacccatag
     1201 ctcagttgga agagtgtcag tttccgaagc tggaggtcac aggttcgatc cctgttgggt
     1261 gcgccaatta taaagagacc gtctgaaaga taaatatttt tcagacggtc ttttgactta
     1321 cttcaaactc ttatttcaag acttccgcaa atgcgcgggc aacatagtcg gtattcgacg
     1381 tattcagtcc ggcgacgcac atcctgccgg aatccagcag gtaaacggca aattcgtcgc
     1441 gcagcctgcg gacttgttcc acgctcaatc ctgtgtagcc gaacatgccg cgctgtttga
     1501 tgaaataagt gaaatcgcga ttggggattt gcgcagttaa gacatcataa agtttctgcc
     1561 gcatcgcacg gatgcggtcg cgcatcatat aaacctcgtt ttgccacaag gcgtaaagtt
     1621 cggggctgtt catcacgtcg gcggcgatat acgcgccgtg cgcgggcggg ctggagtaga
     1681 tgcggcggac ggtgaatttg agctgtccga acaccaaatc cgcttcttcc ttattcgggc
     1741 aaaccacgct taagccgccg acgcgctcgc cgtagagcga caggtttttt gagaaggaat
     1801 tgctgacgaa caagggcaat tccatttcca ccgctttgcg gacggcgtag gcatcgctgt
     1861 ccaaatcgcc gccgaatcct tggtaggcaa tgtccataaa cggaatcagt ttgcgcgttt
     1921 tgatgatgtg caacacttcg tcccattgcc gttccgacat atccacgccg gtcgggttgt
     1981 ggcagcaggg atggaggatc aggacgctgt tttcgggcag ggtgttgaaa aacgcggtca
     2041 tttcgtcgaa tttcacgccg acagtggcag ggtcgtaata tgggtaagtg ccgacctcga
     2101 aacctgcgcc ttcaaaaatg ccgcgatggt tgtcccaagt cgggtcgctg acgtaggcgc
     2161 gcgcttcggg aaaccagcgg tgcaggaagt ccgccccgac tttgagcgcg cccgagccgc
     2221 ccaaggtttg tacggtaacg atgcgccctt gcgcaagcgc gggattgtct ttgccgaaca
     2281 ataaatgctg caccgcgctg cggtaagtgt ccaagccttc catcggcagg tagggcgacg
     2341 gcgcaggcgc ggcggcacgg gcggtttcgg ctcggctcac ggattccaac acgggcattt
     2401 tgccttcgtc gtcgaaataa atgcctatgc tcaaattgac tttttcgggg cgcgggtcgt
     2461 ttttgaaggt ttcgaccaaa ctcaaaatcg ggtcgccggg atagtattcg atgtgtcggt
     2521 acatagtcct taccttttac tttttcaaag gattttcttt ttcaacaata caccactttc
     2581 gatatggtgc gtaaacggga attggtcgaa caaggcggca cgttcgaccg catgggtttc
     2641 cgccaaggtg tccaaattgg tacgcagcgt ttcgggattg caggaaatgt agatgatgtt
     2701 gtcaaactgc gacaccagct tcaaagtttc ctcatcgata ccggcacgcg gcggatcgac
     2761 gaaaatagtg gaaaatgcgt aatccgtcaa agcaataccg ccatccttaa ggcgtttaaa
     2821 ctcacgtttt ccggtatagg cttcggtaaa ttcttcagca gacagacggg cgattttgat
     2881 gttgccgatg cggttggctt cgatattcca ttgcgccgcg ctgacggagg ttttggagat
     2941 ttcggttgcc aaaacacgtt caaacttttc agataaagga agcgtaaagt tgccgtttcc
     3001 gcaatacagt tcgagcaaat ccccatccaa tccttctgct gcaccgcagg cccattcaag
     3061 cattttctga cacacggcgg cattcggttg ggtaaaactg ccttcaattt gccgataacg
     3121 gaaatcccgg ttgccgacct tcaaagtttc cgttacatag tcctgtttta agactatttt
     3181 ctgtcccctg ctccgcccga tgacggaaat atccaactgt tgctgtaacg cttgcgccgc
     3241 ctgcatccac tcattatcaa gccttttgtg gtaaatcatg gtaaccagca tttccccgct
     3301 gagcgtagac aaaaattcga cggcatacca acggtttctc agttcggcag attgggcggc
     3361 aacggcaatc aattctggca tcaggcggtt gacggcttcg gaagccgctt caaaacggtc
     3421 gcaacggatc aggcttgccc cgccagcctt ttgtcctttt tcaaacatgg cataaaacat
     3481 ttcaccgcct tcgtgccaaa tacggaactc ggcacgcata cggtaatgtt tgtccggaga
     3541 ttcgtacact tcccactcag gaacatccaa acctgcaaaa agggttttaa ggtggtcttt
     3601 tttatcttga agctgctgcg tgtagtcgct catatcgcta tcctgaaaaa tcagaccgga
     3661 ttataagccg attcactgca aaacacgcaa cccctttacc ggcatataac ccgttttcct
     3721 ccaactttta tgctaaaatc cgccgtctcc aacataccgc cgacagaagg aaaatatggc
     3781 aggcaacact ttcggacaac tcttcaccgt gaccacgttc ggcgaaagcc acggcgcagg
     3841 tttgggctgc atcatcgacg gctgcccgcc cggactggca ttaaccgaat cagacatcca
     3901 agccgacctc gaccggcgca aacccggcac cagccgccac gttacccaac gccgcgaagc
     3961 cgaccaagtc gaaatcctct ccggtgtctt cgaaggcaaa accaccggca cgcccatcgc
     4021 cctcttaatc cgcaataccg accagcgcag caaagactat ggcaacatcg cccaaagttt
     4081 ccgccccggc cacgccgact atacctactg gcacaaatac ggcacgcgcg actaccgggg
     4141 cggcggtagg agttccgccc gcgaaaccgc cgcccgcgtt gctgccggag ccgttgccaa
     4201 aaaatggttg aaagaaaaat tcggcacgga aatcaccgcc tacgttaccc aagtcggcga
     4261 aaaaaaaatc cggtttgaag gctgcgaaca catttcccaa aatccttttt ttgccgccaa
     4321 ccaaagccaa attgccgagc tggaaaacta tatggacagt gtgcgcaaat cgttggattc
     4381 cgtcggagcg aagctgcata ttgaagccgc caacgtcccc gtcggcttgg gcgaacccgt
     4441 tttcgaccgc ctcgatgccg aaatcgccta cgcgatgatg ggcatcaacg ccgtaaaagg
     4501 cgtggaaatc ggcgcaggtt ttgacagcgt aacgcaacgc ggcagcgaac acggcgacga
     4561 actgaccccg caaggcttcc tgtccaacca ttcgggcggc atcctcggcg gcatcagcac
     4621 cgggcaagac atctgcgtca atatcgccat caaacccacc agctccatcg ccaccccgcg
     4681 ccgcagtatc gacatcaacg gcaaccccat cgaactcgcc acgcacggca ggcacgaccc
     4741 ctgcgtcggc ttgcgcgccg cgccgattgc cgaagctatg ctcgccttgg ttttaatcga
     4801 ccacgccctg cgccatcgtg cgcaaaatgc ggatgttgcc gtggatacgc ccgatatcgc
     4861 ccgctcggac aagtaaaaag atttagccaa aacacgggct ttatactaaa ataccgacca
     4921 ttgccgtgcc gtctgaacgc acgattctta gaggaataca accgaaatga cacaagaaac
     4981 cgctttgggt gcggcactga aatccgccgt ccaaactatg agcaaaaaga aacagaccga
     5041 aatgatcgcc gaccacatct acggcaaata cgatgtgttc aaacgcttca aaccgttggc
     5101 actcggcatc gatcaggatt tgattgccgc tttgccgcaa tacgatgccg cactgattgc
     5161 acgcgtcctc gccaaccact gccgccgtcc gcgctatctg aaagccttgg cgcgcggcgg
     5221 caaacgtttc gatttgaaca accgtttcaa aggcgaagtt acccccgaag aacaggcgat
     5281 cgcgcaaaac catcctttcg tgcagcaggc tttgcaacag cagtccgccc aagctgccgc
     5341 cgaaacgccg tctgttgaag ccgaagcagc cgaatcttcc gcagcagaat aaatccccaa
     5401 atgaaatgcc gtctgaaaac cgatttggtt tcagacggca tttttccgta tgcggcaatc
     5461 acggttcaaa tatcgagttc cgccgtatcg ccttcgcgct ccatccaggc acggcgggcg
     5521 gcggcttcgc ctttgcccat cagtttgacg aagatgtcgc gcgtctcgtc atccgcgcct
     5581 tcggggattt gtacctgcaa caggcggcgg gtgtcagggt gcatggtggt gtctttgagc
     5641 tggtcggggt tcatttcgcc caagcccttg aaacggctga tggaataggc ggtttctttg
     5701 acgccttctt tttgcagccg ctccaaaatg ctgtcgagtt cgttttggtc gagggcgtag
     5761 aatttgcggg caggtttgct tttgccttgt gcgttgacat cgacgcggaa cagcggcggc
     5821 tgggcgacgt agatgtgtcc gtcggcaacc agtttcggga agtggcggta gaataaggtc
     5881 agcagcaaaa cttgaatatg cgagccgtcc acgtcggcat cggacaggat ggcgattttg
     5941 ccgtagcgca ggccgcttaa atcgggatgg tcgttaatac cgtgcggatc gacgccgatg
     6001 gcgacggaaa tgtcgtggat ttcggcgttg ccgaagagtt ggtcggggtg gacttcaaag
     6061 ctgttgagca ctttgccgcg caggggcagg atggcttggg tggctttgtc gcgggcgagt
     6121 ttggctgagc cgccggcgga atcgccttcg acgaggaaga gttcgttttc gcggatgtct
     6181 tcgctttcgc agtcggtcag cttaccgggc aggacggcga cgccgctgcc ttttttcttt
     6241 tcgattttct taaccgaacg catccgcgcc tgcgcctgac ggatggcgag ttcggcgatt
     6301 tttttaccga agtccacgtt ttggttcagc cacaattcca aagggtcgcc cgatacggcg
     6361 gcaacgagtt tcaacgcgtc gcggttggtc agcttgtctt tggtttgacc ttggaactgc
     6421 gggtcgagga cgcgggcgga gaggacgaag gcggttttac cgaacacgtc gtcactttgt
     6481 actttcacgc cgcgcggtag gaggttgtgc agattgatga aattgttgac ggcgttgaac
     6541 acggcttgtt tcaaacctgc ttcgtgcgtg ccgcccagcg gggtggggat gaggttgacg
     6601 tagctttcgt tggcgcacga gccttcttcc agccaggtca gggcaaacgc cgctccttcg
     6661 ccgatgctga aatcgccgtt gtggtcgtct gaaatgtagt tttcgcaaga gaagataggc
     6721 acggcttcct gcgcgtcggc aatcaggtcg gtcagatagc ttttcagacc gtcggggtaa
     6781 tgccaggttt gggtgtgcgc ttcgtcttcg cctttgaccg gacgggtcag ggaaacgcgc
     6841 acgcccggca gcagcacggc tttggcgcgc agcaggcgtt cgagttcggg aatgctgtaa
     6901 ttcgggcttt caaaatattt gccgtccggc cacacgcgca cttcggtgcc gctgtctttg
     6961 acggcgcatt tgcccacttg tgccaacggt tcgaccacgt cgccgccggc aaacacgata
     7021 cggtggattt tgccttcgcg tttgaccgtt acctcaaggc gggtggaaag ggcgttggtc
     7081 acggatacgc ccacgccgtg caggccgcct gaaaaggcat acgcgctgcc gccgtctttt
     7141 ttgttgaact tgccgcctgc gtgcagacgg gtgaacacga gttcgactac gggtacgcct
     7201 tcttcagggt gcagaccgac gggaataccg cggccgttgt cgcgtacgga aagcgaaccg
     7261 tcttcatgaa tttgcacgtc gattgccgta gcgaaaccgc ccaacgcctc gtccgccgcg
     7321 ttatcaatca cttcctgaca gatatgggtc gggctgtcgg tgcgggtgta cataccggga
     7381 cgttctttga ccggttccaa acctttgagg acggtgatgc tggattcgct gtattggttg
     7441 tttttagcca taggaataat ctgaaagtaa gaaaaacaac gctttcagac ggcctgaaag
     7501 cgttgcgttc cgttgtttta ccggttgccg gaaggttggc gggcggcaaa gtcttcataa
     7561 ctttccatac cgcgcaggaa gcgggaagag agttctttca acgcccccaa ataaacgtcg
     7621 cgtttgaact cgatgacttc gtcaacgggc gcccaatatt gatgccaacg ccagccgtca
     7681 aattcggggt ggcgggtggc gcgcaggttg acatcgcaat cccggccggt caggcgcagg
     7741 agataccaaa tctgcttctg cccgcgataa gatccgcgcc attcgcggcg cacccagttg
     7801 ttcggcacgt cgtaacgcag ccagtcgcgc gtccgcccga cgattttgac gtgttgcggc
     7861 agaagtccga cttcttcgta aagctcgcgg tacatcgcgg tttcggggct ttcgcccggc
     7921 ttgatgccgc cttgcggaaa ctgccaagaa tgttcgcgca cgcgcttgcc ccaaaagact
     7981 tcgttacgtt cgttaatcag gataataccg acattggggc gatagccttc cctgtccaac
     8041 acggtgtcgc cctccgttaa attcaatcgt acgattttcc cacaaatcgg gcggttttga
     8101 caaatcagac ggcatggttc aagcatgtcg aaacacagac ggactcggga aaatatctta
     8161 aatttggttt acaataatgt gtttcaaatt attcgggaat cagaccatgt tagatatcca
     8221 attgctccgc agcaacaccg ccgctgtggc ggaacgcttg gcggcgcgcg gttacgagtt
     8281 tgatgccgcg cgtttcaatg cgctggaaga acaacgcaaa gccgtccaag tgaaaaccga
     8341 agaactacaa gcctcgcgta acagcatttc caagcaaatc ggcgcactga aaggtcaggg
     8401 caaacacgaa gaggcgcagg cggccatgga tcaagtcgcc caaatcaaaa ccgatttgga
     8461 acaggctgcc gccgatttgg atgccgttca aaaagaattg gacgcatggt tgttgagcat
     8521 acctaacctg ccgcacgaaa gcgtacctgc cggtaaagac gaaaccgaaa acgtcgaagt
     8581 ccgcaaagtc ggcaccccgc gcgaatttga ctttgaaatc aaagaccatg tcgatttggg
     8641 cgaacctttg ggtttggatt ttgaaggcgg tgcaaaactc tccggcgcac gatttaccgt
     8701 gatgcgcgga caaatcgccc gtctgcaccg cgccttggca cagttcatgc tggatacgca
     8761 cacgctgcaa cacggctaca ccgagcatta cacgccttat atcgttgacg atacgacgct
     8821 gcaaggtacg ggccaactac caaaatttgc ggaagatctg ttccacgtta cccgtggcgg
     8881 cgacgaaacc aaaaccaccc aatacctgat tccgacagcc gaagttaccc tgaccaatac
     8941 cgttgccgac agcattatcc cgtccgaaca actgccgctg aagctgaccg cgcattcgcc
     9001 ctgtttccgc agcgaggcgg gttcgtacgg caaagacacg cgcggtctga ttcgccagca
     9061 ccagttcgac aaagtggaaa tggttcaaat cgttcatccc gaaaaatcat acgaaacgct
     9121 ggaagaaatg gtcggccatg ccgaaaacat cctgaaggct ttggaactgc cctaccgcgt
     9181 gattaccctg tgtaccggcg acatgggctt cggcgcggca aaaacgtatg acttggaagt
     9241 ttgggtgccg gcgcaaaata cctaccgcga aatctcaagc tgctccaact gcgaagattt
     9301 ccaagcccgt cgcatgaagg cgcgtttcaa agacgaaaac ggcaaaaacc gcttggtaca
     9361 tactttgaac ggctccggtt tggcagtcgg cagaaccttg gtcgccgtgt tggaaaacca
     9421 tcaaaacgcc gacggcagca tcaacatccc tgccgcactg caaccgtata tgggcggcgt
     9481 tgccaagttg gaagtgaaat aagccttaac cctgaacata aatgccgtct gaaacttggt
     9541 tcagacggca tttcctttaa acttttaaaa cacgtcagcc gtcggcacga accgcattgc
     9601 cgcaatcgcc ggtctgtccg acctcgcgga tattggacag cgtaacttcc gaaatattac
     9661 ccaacgcctc ttccgtcaaa aatgcctgat ggccggtaaa cagcacatta tgacaagacg
     9721 acaggcggcg gaacacgtcg tcggtaatca catcgttgga tttgtcttca aaaaacagct
     9781 cgcgctcgtt ctcgtacaca tccatgccca atgcgccgat tttccggcgt ttcaacgcct
     9841 caatcgcggc ggcactgtca atcagcccgc cccggctggt gttgataatc atcacgccgt
     9901 ctttcatttt gtcgaacgcc gcttcgttca gcatatagtg gttttccggc gtggaggggc
     9961 aatgcagcgt gatgatgtcc gactgggcat acagctcgtc caaatccaca tattttccgc
    10021 cgagtttttc cacttcgggg ttgcaaaacg gatcgtaagc cagcaggttc ataccgaaac
    10081 cctttaaaat ccgcatcgtt gcgataccga ttttccccgt gccgataacg cccgccgttt
    10141 tgccgtacat attgaaaccg gtcagacctt ccagcgaaaa attcgcatcg cgggtacgct
    10201 gataggcttt gtggatacgg cggttcaacg tcagcatcag accgaccgta tgttccgcaa
    10261 ccgattcggg cgaataggca ggcacgcgca cgactttcaa gcccaactct tcagccgcct
    10321 ttaaatccac attattgaag ccggcacaac gcaacgccac agttttcacg ccaatttgcg
    10381 ccaatttttc caacacgggc cggctgccgt cgtcgttcac aaaaatacag accgcctccg
    10441 caccttccgc cattttcgcc gttttcgcat ccagcataaa atcaaaaaac tccagctcga
    10501 agccgaaatg ccggttggcg cgggtaaaat gttcgcggtc atagcttttc gtaccgtaaa
    10561 tcgcaatctt catcaatatg tccagttgtc gtctatggtt gagaaacggc ataatacact
    10621 gaattcaaac aaatcagtag aatatggtgg attaaaattg attgcatgca cggcatttcc
    10681 atttcaaaac acaaaactca atcgcccatt gccgccagaa gctccgcctg atgctcggca
    10741 atcagggcat cggtgatttc tgccaaatcg ccgtccatca caaaatccag cttgtgcagg
    10801 gtgaggttga tgcggtggtc tgttaccctg ccttggggat agttgtaggt gcggatgcgt
    10861 tcgctgcggt ctccgctgcc gatgagggat ttgcgttcgg cggcttcttt ggcttgggct
    10921 tcgcgttttt gcgcgtcgtt caggcgggcg gcgaggactt tcatcgcctg cgctttgttg
    10981 gcgtgttggc tgcgtccgtc ttggcattcg accaccatgc ctgtgggcag gtgggtgatg
    11041 cggacggcgg aatcggtttt attgatgtgc tgcccgcccg cgccggatgc gcgaaaggtg
    11101 tcgatgcgaa ggtcgacggg gttcaactcg atgtcttcca gttcgtccgc ttcgggcatg
    11161 acggcaacgg tgcaggcgga ggtgtggatg cggccttggc tttcggtggc ggggacgcgc
    11221 tgcacgcggt gtccgcccga ttcaaatttc aaacggctgt acgcgcctaa gccgataata
    11281 cgggcgataa cttctttata gccgcccaac tcgctttcgt tggcggacac gatttcaacc
    11341 tgccagcggt tgcgttcggc gtagcggctg tacatacgca gcaaatcgcc ggcaaacagc
    11401 gcggcttcgt cgccgcccgt tccggcgcgt atttcgatga agatgttttt gtcgtcgtcg
    11461 gtatctttgg gcagcagcag tttttgcaat tcggtatcga gtgcgccgat tttggctttc
    11521 gccgcttcga tttcttcggc ggcaaagtct ttcatttcgg ggtcggacaa catttcttcg
    11581 gcatccgcca aatcgctttg cgccagttga tagttttgga atacttcgac gacgggggtc
    11641 agttcggcgt gttcgcgcgt gagcttgcgg tagttgtcca tatctgccgt ggcttcgggt
    11701 tgccctaaaa ggtgggtaac ttcttccaac cggtcgccga gttgttgcag tttttctaag
    11761 atagacggct tcataattct tccataacaa acgccgcctg aatttcagac ggcatcaaaa
    11821 cgcggtcatt ataatatatt ttctgaatat tcaaaaagat aatctaggga cggagcaggc
    11881 tgtcggcatt gcgttccgtt atcgcggcga tgtccgccac gccgacaccg cgtatctgcg
    11941 cggcaatttc ggcaatccaa agaatattgg caggcgtatt aatctctttt ttcagcataa
    12001 acgggctgtc ggtttccaat acgaaatccc cgtcgttcaa ggctttaagc gtatcgcgca
    12061 ctttacgcgc gttcggattg agcaacaacg aaccgatgcc gattttgaaa cccagtttcg
    12121 tcaacacgcg cgcttcttcc gcgctgcctg agaaggcgtg aacgatgccg ccttgggcaa
    12181 agcctgtctg tttgacggcg gcggcgatgt cggcggtggc tttgagatta tggataatca
    12241 cgcggcggcg cagggtttgc gcgatggcaa gctggcggac gaaaacttgg atttgccgtt
    12301 cgcgctgcgg cggcgtttgg gttttgtcgt aaaaatccaa gccgatttcg ccgacccatg
    12361 cctgcggata acgtgccaac atcgcttcca ggcggacgca atcacgctcg gcaatgccgt
    12421 ctgaaaacca aggatggatg ccgagcgcaa tacggatttg accgtgttcg gacggcattt
    12481 ccgacagttc cgccacgctt tgccaatcct gcggacaggt ggcgggaacg ataaaccgct
    12541 tcaccccaac tttccgcgcg gcggtcagga tgtgcggcag gttttcgcgc agggcggcat
    12601 cggcaaggtg gcagtgggtg tcggtgaagt tcatttcgat tccgcaatgc ggcaggttca
    12661 cataaaaata aggcagggca gcaagggatg ccgtgtacgg gcggcgcata aggcgcggtc
    12721 aacgccccgt cattgccatt tcaatccgct ataacttcag tcttgccaac tctttttcca
    12781 cctcttcctt accccagcct tgcgatacgg cgagggtcat gagtgcggtg gcggtttcga
    12841 ggttgcattt gccgccgttg atgatgcctg agttgcggaa cgcgttgcct tgcgcgtaaa
    12901 cggcagcggt tctgccttgt cggacttggc tgatgttgag cagcagcctg ccttgctgcg
    12961 taaagtcttg cacggcgcgg atgaaacctt cgtctgcggg cgtgttgccg tgtccgtaac
    13021 tttgcaggat aagggcgtgg gcgggaagct gtccgagtcc gtcggcaagt tcttggacgg
    13081 caaagccggg aataagcgtg cggacggcga tttttgcctg cgggtcagga tagcggattt
    13141 tgaggccgtc tgaaacggcc gccgcatctt gggacggaat acgaagattg taccagcctt
    13201 gagtttcatc ccattcggcg atgacaccga aatgcggatt gtcgaagcct gcggcggttt
    13261 cggtgctgac tttgctgctg ccgacggcgg gataaagttt gccgtcaaag gcaataagcg
    13321 tctgcttcaa tccgagtggg aatgcggcaa cggcagtcgc cagattgcgc ggcgcgtcgc
    13381 tgcccacggc gttgtacggc cattgggaac cggtcaggac aatcggtttg accaaaccct
    13441 gcagcgcgag ggcgaacaga ttggcggtat aggcgagcgt gtccgtaccg tgcagcacca
    13501 aaacgccgtc gtactgcggc agcgcggcgg caatgatgtc cagccaagcg caccagtttt
    13561 gcagttggac ggcggaagaa tcaatcagcg tcccacaaac gtgccaatcg aaatccatgc
    13621 cgtttgaaaa cggcgcgagt gcctgcccga ccagcgcggt atcggggcgc aggccttcgc
    13681 tgctttgcgt catgccgatg gtgccgccgg tataaaggac aaaaattttc tgtttcatgg
    13741 ctcatcctga aaatgcaaag accgcctgaa acgcgaaatg cgtccggacg gtcgaaaatc
    13801 tgatttactg ctgcttctgt gccgcttttt tcgctgcctt ttccgccttg cgtttggcgc
    13861 gtttttcgct caattgtgcg cggtaaagct gataatgtcg gcgtttgcgc caccagaacc
    13921 aagccagcgc cgccgccagc acgcccaatg cgatgaagat gccggattgc aggctgtgca
    13981 ttttcgccat cagccaatcg atgttgtgcg cgccgtactc gcccaagtaa atccaaacgg
    14041 gcacggaaat cagcgcggca agcccgtcca taatcagaaa gcgcagatac gatactttgc
    14101 ggctgatgcc ggcggtaacg aaaacggcag tccgcaaacc cggcaggaag cgggcgacaa
    14161 acagaaccca gttgccgtat ttgtcgaatt tttcctgaac ctgtgcgtaa cgtttcggcg
    14221 tcatgatgcg cgcaatcggt ttgaacttga ggattttctg cccccaaatt cgtccggcgg
    14281 cgaacatgat gccgtcgccc gccaacacgc cgagcatacc gactgcaaac ataatatgcg
    14341 gattggtata acccataccc gaaatcacgc cgcctgttac caaggtcaaa tcctcgggaa
    14401 tcggcacgcc gaaaccgcag atgaccaaaa cgaaaaacac ggccgcatag ccgtattcga
    14461 caaaaaaggc ttccaaaagg gcaaacatag cggatattcc attgtcggag ataaaaagtc
    14521 agaacaagcc gaaacatttt ctgcgcgaaa cggcattcta tcaaagatta tgccgtctga
    14581 aagcggcaag gggcagattc gcgccctctg ccggacgttc agacggcatt taaacaggaa
    14641 aaccgccgct gttgaaacgg ttttgcctga ttttgcctaa aggctgccga tggcggaagc
    14701 aatacgttcc gcaccgtcgc gcgcccaatc cgcctgccgc gcctcaacca tcactcgcac
    14761 gacgggttcg gttcccgaag cgcgcaacac gacacgccct ttgccttcga gttctttttc
    14821 cacttccgcc aacacgtctt ttgaggcttc ctgccagttt tgccctttgc cgatacggac
    14881 attaatcatc gtttgcggat acggctgcca atcggcacaa acggtggcga ggtcttggtt
    14941 cagcgtttgc agtgccgcca aaacttgcag tgcggaaata atgccgtcgc cggtgttgtg
    15001 tttgtccata cacaaaatat ggccgctggc ttcgccgccg atgagccagc tgcgttggtt
    15061 cagctgttcc aacacatagc ggtcgccgac tttggcgcgg cagaaatcga cgccctgctc
    15121 tttcagggcg atttccatcg ccatattggt catgaccgtg ccgaccacgc cgccgatgtt
    15181 gataccttct cgggcgcggg ctttggcgat gacgtaaatc aggctgtcgc cgtcgtaaac
    15241 cttaccgttt ttatcgacca tcatcaggcg gtcgccgtcg ccgtctaagg cgatgccgta
    15301 gtcggcttca tgctgtaaaa cggcggcctg gagtgtcttg gtataagtcg caccgcattt
    15361 ttcattgata ttgtagccgt tgggttcgtc gccgatgctg acgacttgtg cgcccagttc
    15421 gtgaaacact ttgggtgcga cgctgtaagc cgcgccgttg gcggcatcga cgaccagctt
    15481 cagcccgcgc aaatcggaat gcgtagggaa ggtggatttg caaaattcga tatagcggtc
    15541 gtccgcgccg ttgatgcggc gtgcgcgacc gagacgggcg gacggttggg ttttcatttc
    15601 gccgtcgatt ttggcttcga tttccaactc gacttcatcg gaaagtttca cgccgccttc
    15661 ggcgaagaat ttgatgccgt tgtcggaata ggcgttgtgc gacgcggaaa tcatcacgcc
    15721 ggcggacagg cgcaacgcgc gggtcagata agccacgccg ggcgtgggca gcggtccggt
    15781 ctgtaccaca ttcacacccg ccgccgtaaa accggccacc aaagcggctt ccagcatata
    15841 gccggaaatg cgcgtgtctt tgccgatgag gacggtcggt ttctggtcgg tgtcgtgctg
    15901 caccaaaacc tgccccgccg catagccgag tttcaatacg aaatcgggcg taatcggaaa
    15961 ttgccccact tcgccgcgca cgccgtccgt gccgaaatat ttttttgcca tgtgttgctc
    16021 cgagaatgtg aaccgttgtc cgagattata cagtcagttt gtgccctgct gtctgcaccg
    16081 ttgatgccgt ctgaagccgc cccgcccttt tcagacggca tgaagtatgc gaaccgctgt
    16141 ttacagattg atgcccaacg cttcccacac cttcaacgcg tccgccgtcg ccttcacatc
    16201 atgcacccgc acgatttgcg cgccgcgcgc cacggaagcc agcgccgccg ccacgctgcc
    16261 gtgtacgcgt tccgccgcgt ctgcctcgcc agtcagctcg cctatcatac gtttgcgcga
    16321 cacgccgatg agcagcggaa aacccgtttc cgccatcaat tcgggtaaat gccgcatcag
    16381 tgcgatattg tgttgcaagg ttttgccgaa gccgaaaccg gggtcgagtg taatgcgttg
    16441 cggggcgatg cctgccgcga tgcattccgc cgcccgcgct ttcaaatacc gcgccacttc
    16501 accgacaaca tcctgatatt tcggattaat ctgcatattc tcaggcaagc cccgcatgtg
    16561 catcaggcaa atgcccgtat ccgcctgaca cgccagcaat tcgagcgcgc cttcgtcggt
    16621 caacgccgcc acatcgttaa taatatcgat gccgccgagt gccaacgctt tttccataac
    16681 caccgtgcgg cgcgtgtcca aactgacggg aacaccccac cccgccactt ctgccaatac
    16741 aggctcaacc cgcgcccatt cttcttcagg agaaacataa tccgcacccg gccgcgtcga
    16801 ttcgccgccg atgtcgagaa tgtccgcacc ctctttcaaa agccgttcgg cgtgcgccaa
    16861 agctgtttgg gcattttgcg aatacgcgcc gccgtcggaa aaagaatcgg gcgtgagatt
    16921 cacgataccc atgattttcg gtttgtccaa accgatttca aaccgtcctg cctgccaaac
    16981 gtgtcgtgcc atctgaactc ctcccaaaat aaaaaacaga ttatatgccg tctgaaaccg
    17041 tcttgtgcgc ttcagacggc accgctattc gggcggcaga cggcatgttg tccgaatgtc
    17101 tgctccgcct ttgaatctgc cggtatgcct gctatccgcc cgacttttca aaacaggttc
    17161 cgacgattcc gcacgcgcct gccgcctttg ccaagccgta caggatttcc tgcggcatat
    17221 cgcggttcca taatcccgta atattcgcaa tcacgggcag atggctgatt tggcggactt
    17281 tcacgatgga ttcgacatcc aaacggtagg gatggtcttt ggtatggctc aacacgcccg
    17341 actcgcctag aatcaggtgg cggttgccgc gcgagacgac atattctgcg gcattcaacc
    17401 aatcttcggc agaatggtgc ttgtctttac acagcaccaa gggaatgttc aggcttccgg
    17461 cttcattcaa tacggcaaga tcggacatca gcccgccgcc caaatacagg atgtccgccc
    17521 ccgcattcaa agccgcttcg acatggcgga cgttgcggac gcgcaccaat acgggtttcc
    17581 ctgcgccatg cgccgatgcg gtctgttccg ccaaccgtct gcaccgtccc cgcccttcat
    17641 ccgcacttga agtgtcgtat aagtttgccg aagtgaaaaa cggatccaga aacactgcat
    17701 ccgcattgcg ccatactgac ggttctgcgg cgatacggac ggtttccccg ccgccgaaag
    17761 ccacgccttt ggcggcaacg cggctgtctt ccgcccgatt ttcccgactg acggttttcc
    17821 atgtatccaa aatgcggacg gctttctcga cctccggcag cgtctgcacc tccctgacgc
    17881 tcaaaaccct atcgtcgccg attgcgccga tgacagtacg ctcgtcgccg tgagaaatgt
    17941 gttctcgcag acctctgctg cggataaagg cgacaacgcc ggcaatgtcc gcttcggcgg
    18001 cacgcctgct catgacaata atcatatttc ctcctgacac aagaaacggc ctgcccaaaa
    18061 taggattttt gcaagccgtg ttatactgtg gcgtgtttta cagattgttc gggctatgga
    18121 tttattatcg gttttccaca aataccgtct gaaatatgcg gtggccgtgc tgacgatact
    18181 gcttttggcg gcagtcgggc tgcacgcttc cgtctatcgc atcttcacac ctgaaaacat
    18241 ccgcagccgc ctccaacaaa gcattgcaca cacacaccgg aaaatctcgt ttgatgcgga
    18301 cattcagcgc aggctcctgc cccggccgac cgtcatcctg aaaaacctga ccattaccga
    18361 acccggcggc gaccggactg ccgtttccgt ccaagaaacc aaaatcggat tgagctggaa
    18421 aaacctgtgg tcggatcaga tacagattga aaaatgggtg gtttcgagtg cggaacttgc
    18481 cctgacgcgc gacgggaaag gtgtttggaa cattcaagac ctgatcgaca gccaaaaacg
    18541 ccaagcctca gtcaaccgca ttatcgtcga aaacagcacc gtccgcctca atttcctgca
    18601 ggaacagctt atcctgaagg aaatcaacct caacctgcaa tcccccgatt cgtcggggca
    18661 gccgtttgaa agttcgggca tactagtttg gggaaagctg tccgtcccgt ggaaaagcag
    18721 ggggctgttc ctttcaaacg gcatcggccc gcccgaaatc tcaccgttcc attttgaagc
    18781 ttccacttcg ctggacggac acggcattac catttccacc accggcagcc cttctgtccg
    18841 cttcaacgcc ggcggagcgg atgccgccgg cctcggcctg cgtgcagaca cttccttccg
    18901 caacctccac ctgaccgccc aaatccctac gctggcactc aggaacaaca gcattaaaat
    18961 tgaaaccgtc aacggcgcat ttaccatcgg cggcgaatat gcccgatggg acggttcgtt
    19021 caaactcgac aaagccaacc tgcactccgg catcgccaac atcggcaacg ccgaaatctc
    19081 cggcagcttc aaaacaccgc gccaccagac caacttctcc ctcaattcgc cgctcgtatg
    19141 gacggaaaac aaagggctgg aggcgccgcg cctgtatgta tcgacccttc aggataccgt
    19201 caaccgcctg ccgcaacccc gtttcatcag ccggctcgac ggttcgctgt ccgtaccgaa
    19261 tctgcaaaat tggaatgccg aattaaacgg cacattcgac cgccaaaccg ttgccgcgaa
    19321 attcagatac acacatgaag acgcaccgca tctggaagcc gccgtcgcac tgcaaaaatt
    19381 gaacctgacc ccctatcttg acgacgtgcg gcaacaaaac ggcaaaatat ttcccgacac
    19441 cctcgccaag ctgtccggcg acatcgaggc gcacctgaaa atcggaaaag tccaacttcc
    19501 cggcctgcaa ctggacgata tggaaaccta cctccacgcc gacaaagacc atatcgcgct
    19561 cagccgtttc aagtcagggc tttacggcgg ccataccgaa ggcggcatca gcatcgccaa
    19621 cacccgtccc gccacttacc gcctgcaaca gaatgcaagc aacatccaaa tccaaccgct
    19681 gctgcaagac ctgttcggct tccacagctt cagcggcaac ggcgacgcgg tcatcgacct
    19741 gaccgcgggc ggcgaaaccc gaaaagagct tatccgctcg cttcagggca gcctgtcgct
    19801 aaatatttcc aacggtgcgt ggcacggtat cgacatggac aatatcctga aaaacggcat
    19861 ttcgggcaaa actgccgaca atgccgcacc cagcacaccc ttccaccgat tcacgctcaa
    19921 cagcgaaatt tcagacggca tcagccgcca catcgatacc gaactcttct ccgacagcct
    19981 ctatgttacc agcaacggct ataccaatct ggatacgcag gaattgtctg aagatgtcct
    20041 tatccgcaac gccgtccatc cgaaaaacaa accgattccc ctgaaaatca ccggtacagt
    20101 ggacaaaccg tccattaccg tcgattacgg caggctgacc ggcggcatca attcgcgcaa
    20161 agagaaacag aaaatcctcg aagacaccct gctggaacaa tggcagtggc tcaaacctaa
    20221 agaaccgtaa acaccctgcg tacaaaaatg ccgtctgaaa cacattaagc ttcagacggc
    20281 attaccgtaa aacctacaac cccaattccc cccaaatccc atcaatctta gccgtaacgg
    20341 cagggtcttt tttgattaca cgcccccatt cgcgttcggt ttcgcccggc cacttattgg
    20401 ttgcatccaa acccattttg ccgccgagtc cgctgacggg gctggcgaag tcgaggtagt
    20461 cgatgggcgt gttttctacc aaaacagtgt cgcgcacggg gtccatgcgc gtggtgaccg
    20521 cccagatgac ttctttccag tcgcgcacgt tcacatcgtc atccaccacg ataatgaatt
    20581 tggtatacat aaactggcgc aggaacgacc agcagcccat catcacgcgc ttggcgtgtc
    20641 cggcgtactg ttttttcatg ccgatcaccg ccatgcggta ggaacagcct tcgggcggca
    20701 ggtagaaatc ggtgatttcg gggaactgct tttgcaaaag cggtacgaac acttcgttca
    20761 atgccacacc caaaacggca ggctcatcgg gcggtttgcc cgtgtaggtc gaatggtaaa
    20821 tcgggttttc gcgcatggtg atgcgttcga ccgtaaacac agggaaatga tcctgctcgt
    20881 tgtaatagcc ggtgtggtcg ccgtacgggc cttccaacgc ggtttcgttc ggatggatga
    20941 cgccttccaa cacgatttct gcgcgggcag gcacttgcaa atcgttgccg atacatttca
    21001 ccagctccgt ccgcgaaccg cgcagcagtc cggcaaactg gtattcgctc aaggtatcgg
    21061 gaacaggcgt taccgcgccc aaaatggtgg cggggtcgca gccgagtacg acggcgacgg
    21121 gatacggcgt atcgggattg agtttgcgga attcctgata atccaacgcg ccgccgcgat
    21181 gcgacagcca gcgcataatc agcttgtttt tgccgattaa ttgttggcgg taaatgccga
    21241 gattttggcg ttttttgtgc ggcccgcgcg ttacggtcaa gccccacgtt accagcggcg
    21301 caacgtcttc cggccagcaa tgctgaatcg gaagtttata caaatcaacg ttttcgcctt
    21361 cccacacgat ttcctgacac ggcgcgtttt tcaccacgtt cggcgccatg ctccaaatgt
    21421 ctttcagtag cggcagtttg gaaaacgcat ctttgatgcc tttgggcggt tcgggttctt
    21481 tcaaatacgc cagcgtctgc ccgatttcac gcagcttgga cacgctgtcc gcgcccatgc
    21541 ccatcgccac acgttcgggc gtgccgaaca ggtttgccaa cacaggataa ccgtagcgcg
    21601 taccgtcggg cttaaccggg ttttcaaaca gcaacgccgg cccttcggcg cgcagcacgc
    21661 ggtcggcgat ttcggtcatt tccaaatgcg gggaaacggg gtgcgcgatg cgtttgagtt
    21721 tgccctgctg ctcgagcatg gcgatgaagt cgcgcaggtc tttgtatttc atattcatcc
    21781 tttttgtcct tttatcctga gcaatccgat tcggataccg cccctatcct tgcctgcgct
    21841 tcggcatatt ctatgccgtg ataaaagtcg cgtaccagcg gatgttcgct gccttgatgg
    21901 agttgcaaca aaggacgttg accatcgggt tgggtaacga cattgcaatg caaaccgaag
    21961 gtgtcggttt cataaggggg cagccggttg cagatcatcc cgaaataaac gacattttcc
    22021 ggattgtcgt gaaagtggct ttgatagtcg ttaaaactct tttcgctgac ggacacccac
    22081 acgccgtatt ccagcgtttc ctcatgcccg ataatcggaa taagcagcac cgcgcggata
    22141 aaacggtcgg tttggtcggg atagcggatg atgcagaaat cgccgccgca ttcagcttgg
    22201 taagcaatgc gttcttcttc actgagttga ttatagggat agggggcggt aaagccgatt
    22261 gcgggcatat cttcatgttt ttcgccgcag gaagtgcaag tgtacataag gtttcagact
    22321 ttcaaaacga gtttgcggta aagccattcg ccggcaaacg gcatccccat caatacatag
    22381 gcaatcacgc cggtataaac cgcccaccaa tcatatcgcc ccaaccgtgc caacaaagcg
    22441 gcaagcgtcc cgttggcaat aaagaatacg caccaaacct gcgttacccg gcgggtatag
    22501 cgcacagctt tttcaggcag gtcgggctgt tgcagccgcg caagtttttc aatcaccgtc
    22561 tgcccggcaa acaggctgcc gccgaacacc gccaacatca tcagattgac gaggacggga
    22621 taccaataca tcgaatcatg ccgcccgaac accaatactg cggcaaaaaa cagtgtaata
    22681 aacaaagccg cataacgctg ttggggcagt ttggcagtca gggcgcgcag cagccacaac
    22741 ccgcacatcg ccgccgccag ccagacaaac cagcccgcct ccctgccgta atatagtgga
    22801 ttaacaaaaa ccagtacagc gttgcctcgc cttgccgtac tgtctgcggc ttcgtcgcct
    22861 tgtcctgatt tttgttaatc cactatacca caaagcggga taggcaatgc ttaatacggt
    22921 cagaaaaata tgtccgaaaa aaccgggttt cattttgaat ccgcacaaat gttttcagac
    22981 ggcatccgat aaaaacatgc cgtctgaaaa ataattagaa atacccgatt agcccgcctg
    23041 aatcttcaat accgcctgta ccacgtcgtt gacggtgcgg acattgcgga aatcttcagc
    23101 ctgcagcttg cgggctgcca cagccattca cgattgcgat atacccggcg gcattcccga
    23161 ctgccgttca gcagaaagcg cacccattcc aaaccgctcc aatatgcctc gggcagcata
    23221 ccggcttcag acggcatatc gtccgaagca gataaagtca ggctgtaacg cgtcccttta
    23281 gtcagaacca tcgccaaagc ataagcaaac ggcgcgcgcg ttgccgatac gacatatcct
    23341 tccggcagcg gatcgtccgc cgccaaaacc aaaaccgacc cgcatccctc ttccaacagt
    23401 gatgccgctt ccgccaatgc cgtttccaca ccgtcggcac acacggccaa tgccgtctgc
    23461 tcgctcatat cccgcctcaa tatcgaccat taccccgccg ttgcattgtg caccgacaaa
    23521 ccgaacgaag tcggcgacac ggtttgcgat ttcaacagtt ccaaccacaa atcgaaactg
    23581 cgcaccattt ccccgtcgtg cgaggcataa actatagtgg attaaatttt aggggctgta
    23641 ctagattagc agatatgtta cccccgaaat atgaagataa cacactgcaa attaaagaaa
    23701 aaagtacaga aagaactgct ccgttttttt gtactcgaag ttaccgcccg ttctgccgcc
    23761 gatattttgg gtatccatcc caattcggca gcactgttct accgtaaaat ccgcacggtt
    23821 atcaaccatc atttggtctt ggctgccgat gaggtttttg agggccctgt cgagcaggac
    23881 gaaagcgatt tcggcggacg gcgtaaaggc agacgtggtc gcggtgcggc aggaaaagtg
    23941 gttgttttcg gcattctgaa acgcaacgga cgggtctata ccgttgtcgt aaacaatgcc
    24001 aagtctgaaa cgttactccc tgtcatcaaa aagaaaatca tgccggacag cattgtttat
    24061 accgatagtc tgagcaactg cgacaagttg gacgtgagcg gttttatcca ttaccgcatc
    24121 aaccattcca aggagtttgc agaccgtcag aaccacatta acggcattga gaacttttgg
    24181 aatcaggcaa aacgcgtctt gcgaaaatat agtggattgg cacgaacgtt tccccgaatt
    24241 ggaagcgcaa ctgggtgcgc cgattgagga ttacgcgccg ccgaaacatt ggacgcgcaa
    24301 gcagctcaga agtatggggc gcgtgtcgta tttgtgcgtc gatgcggcgg agcaggcttt
    24361 gacggatgcc ggtttgctcg gagacgaaag tattaccgac ggacggatgg gcgttgcctg
    24421 cggctcttcc agcggcagca ccaaagacat cggcgatgtg ggcgaattgt tgctgaccgg
    24481 catgtcgcgc aacttcagtg ccaacaccta tgtgcgtatg atgccgcaca ccgccgccgc
    24541 caatatcggc atctttttcg gactgaaagg acgcatcatc ccgacatcga gcgcgtgttc
    24601 gtccggcagc caaggcatag gttatgccta cgaagccatc aaatacggtc tgaccgatat
    24661 gatgctggcg ggcggaggcg aagaattttt cccgtccgaa gtgtatgttt tcgactcgct
    24721 ttatgccgcc agccgccgca acggcgaacc ggaaaaaacc ccgcgcccat acgacgcgaa
    24781 ccgcgacggg ctggtcatcg gcgaaggcgc ggggattttc gtgctggaag aattggaaca
    24841 cgccaaacgg cgcggtgcga taatttacgc cgaactcgtc ggctacggag ccaacagcga
    24901 tgcctaccat atttccacgc cccgccccga cgcgcaaggc gcaatccttg cctttcagac
    24961 ggcattgcaa cacgcagacc ttgcgcccga agacatcggc tggattaatc tgcacggcac
    25021 cgggacgcac cacaacgaca gtatggaaag ccgcgccgtt gcagcggttt tcggcaacaa
    25081 tacgccctgc acgtccacca agccgcaaac cggacacacg ctgggcgcgg cgggcgcaat
    25141 cgaagccgcg ttcgcgtggg gcattgccga ccggaaaagc aatcccgaag ggaaacttcc
    25201 gccccagctt tgggacgggc agaacgatcc cgaccttccc gccatcaacc tgaccggcag
    25261 cggcagccgc tgggaaaccg aaaaacgcat tgccgccagc tcgtcgtttg ccttcggagg
    25321 aagcaactgc gttttactca tcggatgaaa taagtttgtc aatcccaccg ctatgctata
    25381 caatacgcgc ctactcttga tgggtctgta gctcaggggt tagagcaggg gactcataat
    25441 cccttggtcg tgggttcgag ccccaccgga cccaccaatt cccaagcccg gacgtatgtt
    25501 tgggcttttt tgccgccctg tgaaaccaaa atgctttgag aaaccttgat aatgaaaaaa
    25561 gtcagcgtat tgattgttgc tagaaatgaa gcaaaccaca ttcgggaatg tattgaaagt
    25621 tgccgtttcg ataaagaagt tatcgttatt gacgaccaca gcaccgacaa tactgccgaa
    25681 attgccgaga gtttgggtgc aaaagtcttc agacggcatt tgaacgggga tttcggagcg
    25741 caaaaaacat ttgccatcga acaggcaggc ggagaatggg ttttcctgat tgatgcagac
    25801 gaacgctgca cgccggaact atctgatgaa atctcaaaaa ttgtccaaac cggcgattat
    25861 gccgcctatt ttgtcgaacg ccgcaacctt ttccccaacc atcccgccac acacggcgcg
    25921 atgcgtcccg acagcgtatg ccgtctgatg ccgaaaaaag acagttcggt gcaaggcaaa
    25981 gtacacgaaa ccgtacaaac cccctacccc aaacgccgtc tgaagcattt tatgtaccat
    26041 tacacgtacg acaactggga acaatatttc aacaagttca acaaatatac ttccatttca
    26101 gccgaaaaat accgagagca gggaaagccc gtgcgtttcg ttagggacat tatcctccgc
    26161 ccgatttggg ggtttttcaa aatttatatc ctgaacaaag ggtttcttga tggaaaaatg
    26221 ggttggatta tgtccgtcaa ccacagctat tacacgatga ttaaatatgt caaactatat
    26281 tatctgtaca aatccggcgg aaaattttaa atggaaaaag aattcaggat attaaatatc
    26341 gtatcggcca agatttgggg tggaggcgaa caatatgtct atgatgtttc aaaagcattg
    26401 gggcttcggg actgcacaat gtttaccgcc gtcaataaaa ataatgaatt gatgcacagg
    26461 cgattttccg aagtttcttc cgttttcacg acacgccttc acacgctcaa cgggctgttt
    26521 tcgctctacg cacttacccg ctttatccgg aaaaaccgca tttcccacct gatgatacac
    26581 accggcaaaa ttgccgcctt atccatactt ttgaaaaaac tgaccggggt gcgcctgata
    26641 tttgtcaaac ataatgttgt cgccaacaaa actgattttt atcaccgcct gatacagaaa
    26701 aacaccgacc gctttatttg cgtttcccgt ctggtttacg atgtgcaaac cgccgacaat
    26761 ccctttaaag aaaaataccg gattgttcat aacggtatcg ataccggccg tttccctccc
    26821 tctcaagaaa aacccgacag ccgttttttt accgtcgcct acgccggcag gatcagtcca
    26881 gaaaaaggat tggaaaacct gattgaagcc tgtgtgatac tgcatcggaa atatcctcaa
    26941 atcaggctca aattggcagg gcacggacat ccggattata tgtgccgcct gaagcgggac
    27001 gtatctgctt caggagcaga accatttgtt tcttttgaag ggtttaccga aaaacttgct
    27061 tcgttttacc gccaaagcga tgtcgtggtt ttgcccagcc tcgtcccgga ggcattcggt
    27121 ttgtcattat gcgaggcgat gtactgccga acggcggtga tttccaatac tttgggggcg
    27181 caaaaggaaa ttatcgaaca tcatcaatcg gggattctgc tggacaggct gacacctgaa
    27241 tctttggcgg acgaaatcga acgcctcgtc ttgaaccctg aaacgaaaaa cgcactggca
    27301 acggcaggac atcaatgcgt cgccaaccgt tttaccatca accataccgc cgacaaatta
    27361 ttggatgcaa tataaactgc tttcagacgg catatgccgt ctgaaagcct ttgatgcaac
    27421 aaaccgccaa attatattcg ttcattggaa agaaacaccc cgaatccatc cttaaaaata
    27481 agaaaatccc aatatccccc gatattacgc agcctattgg caaagttttg cagcgtcttc
    27541 cccggcttgt gctgccgcat caagtgcttt gttacaatgt atagtagact aaca
//
LOCUS       DAXHRI010000009        27517 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXHRI010000009 DAXHRI010000000
VERSION     DAXHRI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27517
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.009"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(113..516)
                     /locus_tag="ACFM75_000279"
                     /pseudo
     CDS             complement(113..516)
                     /locus_tag="ACFM75_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(673..3423)
                     /gene="gyrA"
                     /locus_tag="ACFM75_000280"
     CDS             complement(673..3423)
                     /gene="gyrA"
                     /locus_tag="ACFM75_000280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC6254880.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVIMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAYPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            complement(3521..4021)
                     /gene="hscB"
                     /locus_tag="ACFM75_000281"
     CDS             complement(3521..4021)
                     /gene="hscB"
                     /locus_tag="ACFM75_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC6254881.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPEFLMQQMEWRET
                     LMEARAGKDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            4103..4324
                     /locus_tag="ACFM75_000282"
     CDS             4103..4324
                     /locus_tag="ACFM75_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC6254882.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRYKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            complement(4284..4604)
                     /gene="iscA"
                     /locus_tag="ACFM75_000283"
     CDS             complement(4284..4604)
                     /gene="iscA"
                     /locus_tag="ACFM75_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC6254883.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4691..5077)
                     /gene="iscU"
                     /locus_tag="ACFM75_000284"
     CDS             complement(4691..5077)
                     /gene="iscU"
                     /locus_tag="ACFM75_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC6254884.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(5435..5722)
                     /locus_tag="ACFM75_000285"
     CDS             complement(5435..5722)
                     /locus_tag="ACFM75_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254885.1"
                     /translation="MNKILEKLIEQACTNNALFCGKLLTDLTKDEMDILSSFHAIDVD
                     MIVLNDDYFCGIRADHFVIEFGWSECHEGDLILITANHKGSRALTLIDISK"
     gene            complement(5875..7089)
                     /locus_tag="ACFM75_000286"
     CDS             complement(5875..7089)
                     /locus_tag="ACFM75_000286"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC6254886.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLGHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTSEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(7118..7564)
                     /gene="iscR"
                     /locus_tag="ACFM75_000287"
     CDS             complement(7118..7564)
                     /gene="iscR"
                     /locus_tag="ACFM75_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC6254887.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            7914..9086
                     /locus_tag="ACFM75_000288"
     CDS             7914..9086
                     /locus_tag="ACFM75_000288"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC6254888.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(9235..12006)
                     /locus_tag="ACFM75_000289"
     CDS             complement(9235..12006)
                     /locus_tag="ACFM75_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6254889.1"
                     /translation="MANNKTLFEVIENERKAVKKYKPELLEMPEFTSKNLKYDFFEWQ
                     KSALENFLIFDRTSELDDFPDLKNKPTHLLFNMATGAGKTMMMAALILYYFEKGYRHF
                     LFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKFT
                     SIQKLYNDIHTERENQTTLADLHDLNLVMLGDEAHHLNAQTKGKKQGELDLEKEMNDR
                     TSHAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATPPEKADVQQKYADKIITKFGL
                     KEFLQKGYTKEINLVSSTLSKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTV
                     DESKADYLAFLNWVENVQADDFSFLKTFSMSLNDSDNANEQGKTRTEQALKFMQENGF
                     EFAHLANWIKQNYQKHNIIITNSETNKSKTEKTDSETEKLLNNLEAVDNPIRAIFTVG
                     RLTEGWDVLNLFDIVRLYEGQNGGGSNKKSCKTANTTVSEKQLIGRGVRYFPFSFEDK
                     QPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLATF
                     KLKSEFADNENFKNLLIWANKKIPNPNAKSNNADSLKANPQTLSFQIHGNQLLQETQF
                     TADKNDEKVKQLGIQNNFSQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLNI
                     QNRNELQNNLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDKPFIGTK
                     EFTPKTLWEIFGTPKQKWVKKDDIKTAIAMQNDWYVMDNFAGTSLEEALIQFISARLG
                     DLKSKYDVHLIRNEEVFKLNNFADSEGFMPDFVLLLKDKQKSSSDSVDGFLHYQIFIE
                     PKGEHLVETDRWKEEFLEAITAEYGKDKILQKDTPHYRLIGLPFFTDHQKNGQFTESF
                     PLGAASLEK"
     gene            complement(11996..14179)
                     /locus_tag="ACFM75_000290"
     CDS             complement(11996..14179)
                     /locus_tag="ACFM75_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFC6254890.1"
                     /translation="MKTDIQAELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLGNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSSEESEEIYFKHSNSQPASQPASQPASQPASQPASQL
                     YTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRHTADGKQAVGEIKRHSDGTPAE
                     NLIIKGNNLIVLHSLAKQFKGKVKLIYIDPPYYFVKKKQQDSFGYNTNFKLSTWLTFM
                     KNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFIGNLPTIMNLKGNNDE
                     YAFAGTHEYTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGFYKKGAPMRATGTEDK
                     REDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQKLKEKYENLGYNFIL
                     PIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIFY
                     KPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNENDIVLDYHLGSGTTAAV
                     AHKMNRQYIGIEQMDYIETLAVERLKKVIEGEQGGISKAVNWQGGGEFVYAELAPFNE
                     TAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQEPEFQSLPLDEQKQMV
                     LEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSRAFYQAEKKDGE"
     gene            complement(14499..15419)
                     /gene="truB"
                     /locus_tag="ACFM75_000291"
     CDS             complement(14499..15419)
                     /gene="truB"
                     /locus_tag="ACFM75_000291"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC6254891.1"
                     /translation="MTNKPTKRPVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIVATARADI
                     SLAEFQTACQALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRAETAGFTIAQSHTLEAL
                     ANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLRCGQRPRFEEDLPSDTPVRVYTE
                     TGEFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(15477..16001)
                     /locus_tag="ACFM75_000292"
     CDS             complement(15477..16001)
                     /locus_tag="ACFM75_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254892.1"
                     /translation="MNNLIPEHLAAYAHSDSLQIEGVHRCFSLSCQGRDTFHIRYYGE
                     PFDGLMTDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSPNQKQNRTLTDL
                     DEYTYRVLIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISVDLID
                     EKHSVRELLNEELS"
     gene            complement(16012..16383)
                     /gene="rbfA"
                     /locus_tag="ACFM75_000293"
     CDS             complement(16012..16383)
                     /gene="rbfA"
                     /locus_tag="ACFM75_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC6254893.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            16573..17817
                     /gene="clpX"
                     /locus_tag="ACFM75_000294"
     CDS             16573..17817
                     /gene="clpX"
                     /locus_tag="ACFM75_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC6254894.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPPESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAEGKVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(17920..19116)
                     /locus_tag="ACFM75_000295"
     CDS             complement(17920..19116)
                     /locus_tag="ACFM75_000295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC6254895.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDQHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDTAEGLRRLEHALTEFA
                     ATSDNP"
     gene            19520..20020
                     /locus_tag="ACFM75_000296"
     CDS             19520..20020
                     /locus_tag="ACFM75_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254896.1"
                     /translation="MNAMPQTAAQNETLHICSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            20105..20659
                     /locus_tag="ACFM75_000297"
     CDS             20105..20659
                     /locus_tag="ACFM75_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254897.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            20673..20873
                     /locus_tag="ACFM75_000298"
     CDS             20673..20873
                     /locus_tag="ACFM75_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254898.1"
                     /translation="MPSEGLSDGIFAFVRGIVMLPFDFQTAFCFPAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(21148..22536)
                     /locus_tag="ACFM75_000299"
     CDS             complement(21148..22536)
                     /locus_tag="ACFM75_000299"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6254899.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
     gene            22864..23811
                     /locus_tag="ACFM75_000300"
     CDS             22864..23811
                     /locus_tag="ACFM75_000300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6254900.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMQPEEYEAWLAEVLEAVVFVTGFAPEQIHLKRRERQKGLQQ
                     YEKTGKTGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(23864..24196)
                     /gene="trxA"
                     /locus_tag="ACFM75_000301"
     CDS             complement(23864..24196)
                     /gene="trxA"
                     /locus_tag="ACFM75_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC6254901.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            24494..24967
                     /gene="dnrN"
                     /locus_tag="ACFM75_000302"
     CDS             24494..24967
                     /gene="dnrN"
                     /locus_tag="ACFM75_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC6254902.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGIGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDDFQVPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            complement(25037..25858)
                     /gene="nadE"
                     /locus_tag="ACFM75_000303"
     CDS             complement(25037..25858)
                     /gene="nadE"
                     /locus_tag="ACFM75_000303"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC6254903.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDKYAAQANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQGQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            26055..27410
                     /gene="xseA"
                     /locus_tag="ACFM75_000304"
     CDS             26055..27410
                     /gene="xseA"
                     /locus_tag="ACFM75_000304"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC6254904.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTVVQGTGSELQIAQVIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRTYIHKLAQTLSYSMTQNLRA
                     HAARFERQTQALQHYRPDISVCKKNIDRFQTALPAAFSQLLARRRQSLSAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT         6454 a         6556 c         6960 g         7547 t
ORIGIN      
        1 tgttaatcca ctatagttta aagctttgtt tcttaagtcc gcagagtatg ccatggttag
       61 accttcaaag ttgagtattg tactattttg tttatggggc tgtcctagat aactaggata
      121 aactcgattt tactaattgt tttaaaatgg aaatttgaac ttttatctca ctgttgttaa
      181 aacgccgttc gtaccccttt aaatacagct caaaatgcgc tttgggaatg ccgtcaaact
      241 tgcgtaaatg acgttttgcc cggttccaaa agttcccaat tccattgata tggttttgtc
      301 gttcagcaaa ataactttca tctgcttcta ctttgccgtc aaacatttcc aaatgcggac
      361 tgttttgata aataagtaat cgtaaacgat gaaaataata ggctgcggta tttttattaa
      421 cgcctactaa ctctgctgtc gttcttgcag ttacacctgc gacaaacagt tcaatgagtt
      481 tatttgttta taccggctta gacgactttt tctcataggg gcaactctaa cttaatttga
      541 atttccctag ttatctagga cagccccttg tttttaattg actataatcc gctatattgt
      601 gagaagctgg atgacggatg agggataaaa taaatgccgt ctgaatcttc agacggcatc
      661 gggagatgat tttcagttct cgacttccgg ttcggttaca ttggaaatta cagaagcgtc
      721 ggagagttcg gattcgtctt cggcaacacg ttccagcgat accaaggttt cgccttcgtc
      781 caagttaatc agtttcacgc ctgctgcggc gcggccggtt tcgcggattt gttcgacttt
      841 ggtgcggata agtacgccgc cgctggtaat cagcatcaaa tcgtcggttt cgccgaccaa
      901 ggttgcggcg accaaatcgc cgtttcgctc gccggtgtta atggcaatat tgccttgccc
      961 gcctttgttt ttgcggctgt aatcggcaat cggggtgcgt tttccgtatc cgttggcggt
     1021 ggcggttaaa acttgcaaac cgctttcttc ggtttcaggg gcgaaggtaa tcaggctgac
     1081 gattttgccg tcggcaggca ggcgcatacc gcgcaaaccg ccgctgccgc gaccggacgg
     1141 gcgaacaccg tgtttgccgc tcggcagtgc gttttcgctg tcggcggttt catcttcgat
     1201 gccgtctgaa atttcggttt cgatgtcggc atcttccgct tcgtcgttgc cggatttttc
     1261 ccagtattcg ttgaagcgga tggctttacc taagttggag aacagcatga tgtcgtccgc
     1321 accgcctgtt tgcgcagcgc cgacgaggta gtcgccttct ttgagcgcga tggctttaat
     1381 gccttgggcg cggacgtttt taaaggcgga aagttggact tttttcacca ttccctgcgc
     1441 ggtggcgaag aagacgtatt ggtcttcggg gaactcgcgt actgccagaa tcgcgctgac
     1501 tttttcgcct tcttccaact ggatgacgtt gttaatcgga cggccgcggc tgttgcgtcc
     1561 gccttcgggc agtttgtaaa ccttaatcca atgacacttg cccaaattgg taaagcacat
     1621 caaataatca tgcgtgttgg caacaaacag ggtttcgata aagtcttcgt ctttggtggc
     1681 agccgcctgt ttgccgcgcc cgccgcgacg ctgcgcctga tagtcggtgg tcggctgggt
     1741 tttgatatag ccgccatgtg tcagggtaac gaccatttcg cgttgcggaa tcaggtcttc
     1801 atcggcaatg tcgccaccga acgggttgat ttcgctgcgg cgttcgtcgc catagttggt
     1861 tttgatttct tccagttcgt cgcggatgat ttgggtaatg cgttcgggtt tggagaggat
     1921 atccacaaag tcgatgattt tacccatcag gtttttgtag ctttcgacaa tttcttcttg
     1981 atcgaggccg gtcaggtttc gcaggctcat gcgtaaaata gcatctgcct gaatctcgct
     2041 caggtaataa ccttgttctt tcaagccgat gtttgcagcc aatccttccg gacgcatcat
     2101 ttccaaatcc agaccggaac gcgtcagcat ttcttcaacg aggctgctgc gccaagggta
     2161 cgcaagcagt ttgtctttgg cctcggctgc gttgggcgat tctttgatga gcttgatgat
     2221 ttcatcgata ttggacagtg cgacggcttt gccttcggca atatgccctt catggcgtgc
     2281 cttcttcagc cggaaaagcg tacgtcgggt aacgacttcg cggcggtggc gcaggaattc
     2341 ggagagaatc tgtttcaggt tcaataggcg cggttgtccg tcgaccaaaa ccaccatatt
     2401 gatgccgaaa ctgtcttgca gcggagtcag tttgtagagt tggtttaaga cgacttcggc
     2461 attttcgttg cgtttcagct cgataacgac gcgcataccg gatttgtcgg attcgtcgcg
     2521 gagctcggaa atgccttcca gtgttttttc ccgaaccaaa tcgccgattt tctcgaccag
     2581 cttggctttg ttgacctgat aggggatttc gtcgataacg atggcttcgc gttcgccgtt
     2641 tttgcctatg ggttcgatat gggtcttacc gcgcataatg acgcggccgc ggcctgtttt
     2701 atagccttcg cgcacgccgc tcaagccgta gatggttgcc ccggtcggga agtcgggggc
     2761 ttggataatg tcgatcagtt cgtcgatttc ggtgtcgggt gcatcgagca ggcgcaggca
     2821 ggcattgacg gtatcggaaa ggttgtgcgg cgggatattg gtcgccatgc cgacggcgat
     2881 gccggacgag ccgttgacga gcagtgtggg gaaacgggtc ggcagtacaa gcggctcgtg
     2941 ttcgctaccg tcgtagttcg ggccgaaatt gacggtttct tcctcaatgt ctgccagcat
     3001 ttcgtgggaa attttcgcca tgcggatttc ggtgtagcgc atggctgcgg cggcaagccc
     3061 gtccaccgat ccgaagttgc cctgtccgtc tatcagcaca taacgcatag cgaaattttg
     3121 cgccatacgg acgatggtgt cgtatacggc ggtatcgccg tgggggtggt atttaccgat
     3181 gacgtcgccg acgatgcgcg ccgatttttt gtaggcggca ttccagttgt ttttcaattc
     3241 gtgcatcgcg tacagtacgc ggcggtgtac cggcttgaga ccgtcgcgaa cgtccggcag
     3301 cgcgcgcccg acaatgacgc tcatggcgta gtcgagatag cttttgcgca tttcgtcttc
     3361 aaggcttacc ggcagggttt cgagggcgaa tttgtggtcg tggcggatgg ttgcgtcggt
     3421 catggtttca atgtttcgta tggcaaaaaa ttgttgctta ttttagcata ttttgacgcg
     3481 gaacggtgcg gcggttacgc cgtctgaaac acggtgcgga ttataatgcc gaggaaattt
     3541 cgttgcggag tttgtcgaga aacctgcctt ggcggacttg ttgtgcggcg gtgtcgtaat
     3601 cttgtcgggc aaacgactgt ttcagaccgc agaacagttt ttcttgttcg tcgcggattt
     3661 cgttgtcgag atttttcaag gattcaaggt ctttgcccgc ccgtgcctcc atcagcgttt
     3721 cgcgccattc catttgctgc ataaggaatt cgggggcgaa agaggtatgc tccggcgcgt
     3781 cggcatcgat gcccgatgtt ttcagcaggt aggcggcgcg gtcgatgggg tttttcaagg
     3841 tgcggtaggc atcgttgatg gtggaagaca tcatcactgc ctgcttttgc tcaaaggcgg
     3901 aagctgaagc gaatttatcg ggatggaaac gggcggccaa ggcgcggtag gtttgttcca
     3961 agttttcggt gtcgatatcg aaagcgggtt caatccggaa gagggtgaaa tattgggaca
     4021 tagtaggatg ataaatgtaa gattttggca gaaaactgtt tttgccttat aatctgcagc
     4081 ttcttaaacg aaaggactga atatgggcgg caaagtgcag cacaataaag gcaaaatacg
     4141 cgacaatgct ttaaaagcct tagtgaaatc cgatttgttc cggtacaagg tggaacggaa
     4201 aaggaaaggc aaaggcagct acaacaggca ggaagcgaaa aaatggcggg acggttttga
     4261 tactgtcccg ccatttttat gtcttaaaca tggaagcttt cgccgcagcc gcaggagtct
     4321 ttgacattgg ggttttcaaa tttgaaacct tcctgcaaac cttctttggt gtaatcgact
     4381 tgtgtgccgt ccagataaac caggcttttc gggtcgatat aaatgcgtgc gccgtgtcct
     4441 tcaaaaatca ggtcgtcgcc atcggcttca tcgacaaatt caaggttgta cgccatcccc
     4501 gagcagccgc tggttttcac acccaagcgt acgcccaagc ctttgccgcg tttggcgaga
     4561 tagtcattga tgtgttttgc ggcattctcg gtaagggtaa tcatatttct tccttgttgt
     4621 accgccccgg acagacctga agcggcgggt ggttcggacg gcattgcggg acgatgccgt
     4681 ctgaagggct ttatctgttt tcctgacgtt tgcggtagtc ggcaacggcc gcttttaccg
     4741 catcttcagc caagatggag cagtggattt ttaccggcgg caattccaac tcctcggcga
     4801 tttcgctgtt tttgattgcc agcgcgtcat ccagactttt gcctttgacc cactcggtaa
     4861 tcaggctgga cgaagcgatg gcggaaccgc agccgtaagt tttgaatttc gcatcttcga
     4921 tgatgccttc atcgttcact ttgatttgca ggcgcatcac gtcgccgcag gccggtgcgc
     4981 cgaccatgcc ggtgccgaca gattcgtcgt ttttgtcgaa agtgccgacg ttgcggggat
     5041 tttcgtagtg gtcgattact ttatcgctgt atgccatgat gtggtttcct taatgttttg
     5101 atgatttaag tggtttgttt gccgattttc agacggcctg aagtttagat tttgcacgcc
     5161 ccgccttcgc agccgtcgtc gttttccgaa tcgacgccgc cttccacggt aacgccgtca
     5221 aaatcttcaa gcaggttgtc gaggtcaagt tcttgttcgt tcatgggttg catccttgat
     5281 ttgaaggttg tggcttttag gtcgtctgaa aaggtttttg tggattttca gacgaccttt
     5341 ttctatttat taagtaggct gggttgttaa acccaacatt tcgagcagat tgataggctt
     5401 gttgggtttt caacccaacc tacgctttta tgcctcattt tgatatgtct atcaatgtta
     5461 aagctctaga tcctttatgg ttagccgtta ttagaattaa atctccctca tggcattcac
     5521 tccatccaaa ttcaataaca aaatgatctg ctctaattcc acaaaaataa tcatcattca
     5581 aaacaatcat atctacatca atagcatgaa aactacttaa tatatccatt tcatcttttg
     5641 ttaaatcggt tagcaatttt ccgcaaaaca atgcattatt tgtgcaagcc tgttcaatta
     5701 atttttccaa tattttattc atagctatat tcctttaaaa ataaagatgg gaatccatca
     5761 tagtttggga aattataaaa tgttattaag tagactagct catttttggt attagctaaa
     5821 taaaggtcgt ctgaaaaggt ctttttagga ttttcaaacg accttttctc tgggttaatg
     5881 cgccgcccat tcaatcgagt tcaaatcaat cccatcttta aacatttccc acagcggcga
     5941 cagttcgcgc agtttgccga ttttggattt aatcagttcg gcggcgaatt gcacttcttc
     6001 ttcgctggtc atgcgaccga aggtgatacg cagggatgag tgcgccagtt cgtcgttgcg
     6061 gccgagcgcg cgcaggacgt agctgggttc gagcgaggcg gaggtgcagg cggagccgct
     6121 ggatacggcg agttctttca ccgccataat caggctttcg ccttcgacga agttgaagct
     6181 gacgttcagg ttgttcgggg cgcggtgtcc gaggtcgccg ttgatgtaga cttcttcgat
     6241 gccttcgatg cctttgagga agatgtcgcg cagtttgcgg tagtgcgcca tatcttgttc
     6301 gagttcttct ttggcgatgc ggaaggcttc gcccataccg acgatttggt gggtcggtaa
     6361 tgtgccgctg cggaagccgc gttcgtggcc gccaccgtgc atttgggctt cgaggcggac
     6421 gcgcggtttg cggcggacgt acagcgcgcc gatgcctttg gggccgtaca ctttgtgcgc
     6481 ggacatcgag agcaggtcga ttttggcggc ttcgacatcg acaggcactt tgccgcaggc
     6541 ttgggcggca tcgacgtgga agacgatttt acgttcgcgg cagatttcgc cgatggcggg
     6601 aatgtcttgc accacgccga tttcgttgtt tgcccacatt acggaaatca ggatggtgtc
     6661 gtcgcggatg gcggctttga gttcttccaa atcaatcaaa ccgttttctt gaacgccgag
     6721 gtaggttact tcaaaacctt ggcgttcgag ttcgcgcatt gtatctaaaa cggctttgtg
     6781 ttcggttttg acggtgatga ggtgtttgcc tttggttttg tagaagtttg ccgcgccttt
     6841 gatggcgagg ttgtcggact cggtcgcgcc gctggtgaag acgatttctt tggagtcggc
     6901 attaatcagg gcagcgatgt cggcgcgggc tttttcgacg gcctcttctg cttcccagcc
     6961 gaatgcgtgg ctgttggagg cggggttgcc gaaagtttcg gtcagatagg gaatcatttt
     7021 ttcggcaacg cgtttgtcaa cgggggtggt ggcggcgtag tcgaggtaaa cgggggtttt
     7081 gacggtcatg gtttgctctt tctttttcgg gtgttattta atggatgtgt gtaaattgga
     7141 cgacgcggct gccgtcgccg ttgtttttct gttcgatgat gctttgcagg gtaacgctgc
     7201 cgaggtagtc gttgatggtt ttgtttaaat tctcccaaag atcgtgcgtc aggcagggcg
     7261 cgccgtggtg gcagttggct ttgctgccgc attgggttgc gtccagccgg tcttcggcgg
     7321 cggcgatgat ttgggcgatg ttgatgcgtg ccgccggtgc ggcgaggatg tagccgccgc
     7381 cgggcccgcg caggctttca accagtccgg cgcggcggag tttgccgaac aattgctcga
     7441 gataggagag ggatatgttt tggcgttcgc tgatggcact gagtttgacg gcgccggttt
     7501 gcgcgttcat cgccaaatcc agcatagcgg taacggcgaa acgccctttg gtggtcagtc
     7561 tcatggtggt ggttgcccgt gtcggctttt ttatagtggg gagaattgtc caatatctga
     7621 gtgtttcagt caagtaaggg ggagctgttg ccgattgttt gtttaaatgg cggtatagtc
     7681 tgattttgct ttatttgttt ggttttttaa atcagttggt tatgtttttt gaggagcggc
     7741 ggtcggggta ttttgccggc aagcggtttc aaaacgattt gatttatttt tgatattgat
     7801 atagattatc taaatcttag tgatttattt gggtattgcc tgaaatgctg tctgaaggct
     7861 caagcggttt cggacggcat attccatcag gctgtcaaac agggaaaaac aagatgaaac
     7921 gtgatttgag caaaatgacc tgcatcgaag acttgcgccg cgttgccaag cgcaaaatgc
     7981 cgcgtatgtt ttacgattac atcgattcgg gttcgtggac ggaaacgact tatcgggaaa
     8041 atacttcgga tttcaaagac atccgcttcc gtcaaaaagt attggtcaat atggaaggcc
     8101 gaagccttga gactaaaatg atcgggcagg atgtgaaaat gccggtggcg attgcgccga
     8161 cgggttttac cggcatggca cacgcagacg gcgaaatttt ggcggcgcgg gcggcggaga
     8221 agttcggtat tccgtttacg ttgtccacca tgtccatctg ctcgattgaa gacgttgccg
     8281 agaacaccag cgcgccgttt tggtttcagc tttatgtgat gcgcgaccgc gagtttatgg
     8341 aaaacctgat taagcgcgcg aaggatgcca aatgttcggc attggttttg accgccgatt
     8401 tgcaggtttt ggggcaacgc cacaaagaca tcaaaaacgg cctgtccgcg ccgccgaaac
     8461 cgaccatcgc caatttaatc aatctggcga ccaaaccgga atggtgcatg aaaatgctga
     8521 acacggaacg ccgcacgttc cgcaatatcg tcggacacgc gaagaacgtc ggcgatttgt
     8581 cttcgctgtc ttcatggact tccgaacaat tcgacccgcg cctgagctgg gacgatgtgg
     8641 cgcgtattaa agatttgtgg ggcggcaagc tgattatcaa aggcattatg gaacccgaag
     8701 acgcggaaaa agcagcgaaa agcggcgcgg acgcattgat tgtttccaac cacggcggcc
     8761 gacagctcga cgataccgtg tctgccatca aagccttgcc cgacatcgtc agcgcagtgg
     8821 gcagcgacat cgaagtttgg atggacagcg gtatccgcag cggtcaggat attttgaagg
     8881 cgtgggcttt gggcgcgaaa ggtacgatga taggccgggc gtttctgtat ggtttgggtg
     8941 cgtatggtga agaaggcgta acccgtgcgc tggaaatcct gtataaagaa atggatatat
     9001 ctatggcgtt tacgggacac cgcgatattc aagatgtcga tgcttcgatt ttgagaggca
     9061 aagattgggg cagggaaacg gtttagatac tgaaaaatac gatgccgtct gaacacaggg
     9121 ttcggacggc attaaaattt ggctgtattt taataatgtg ttgattaaat atctgacaaa
     9181 acgccctttt gtaaaggaag ttttgtcaaa cggggttgcc tgcaatgcac tactctattt
     9241 ttcaagcgat gccgcaccta aagggaatga ctccgtaaat tgtccatttt tctgatgatc
     9301 agtaaaaaac ggtaaaccga tcaaacgata atgcggtgta tctttttgca ggatcttatc
     9361 cttgccatat tctgctgtga tggcttctaa aaattcttct ttccacctat cagtttccac
     9421 taaatgctca cctttgggct caatgaaaat ttggtaatgc aaaaagccgt ccacgctgtc
     9481 ggaagaagat ttttgtttat ctttcagcaa taaaacaaag tccggcataa atccttcact
     9541 atcggcaaag ttattcaatt taaacacttc ttcatttcgg attaaatgca cgtcatattt
     9601 agatttcaaa tcgcccaaac gcgctgaaat aaattgaatt aacgcttctt ccaaacttgt
     9661 tccagcaaaa ttatccatca cataccaatc attttgcatg gcaatagcag tttttatatc
     9721 atcttttttg acccattttt gttttggcgt gccgaaaatt tcccataatg ttttaggcgt
     9781 aaattctttt gtaccgataa atggcttatc actttcattc aaatgctttt caaccatttc
     9841 caagattttc aggcagcctg caagtttatc atccgggcga acctgtttat cttgctctaa
     9901 tcctaaaaat tcaatttgcc agtctttcaa caagttattt tgcaattcat tgcgattttg
     9961 aatgttgagt ttgctttgca aacggttgaa atggaataaa gattgactat ttttcccttt
    10021 gatatgtaat gctttattga agatgtgccg ttccatttca ctcattttta tggtttgaga
    10081 aaaattattt tgtatgccaa gttgcttgac cttttcatca tttttatcgg ctgtaaattg
    10141 cgtttcctgc aacagttgat tgccgtgaat ttggaatgaa agtgtttgcg gattggcttt
    10201 taggctgtct gcattattac ttttagcatt gggattgggg atttttttat tcgcccaaat
    10261 caataagttt ttaaaatttt cattatctgc aaattcagat ttgagtttaa atgttgccaa
    10321 taccttatca tcgtcttttt caggcaaata accgtctttt cgtaactcgt ttttcagttc
    10381 tgtaatatag cgggattgct catcgtgcgt gtaataaaac aattcttcca aaatacgcaa
    10441 ttcgtgttgc atgtcgttgt caaatttacg cttattcggc tgtttatctt caaacgaaaa
    10501 tggaaaataa cgcacgcctc gaccaatcaa ctgtttttct gataccgttg tatttgccgt
    10561 tttgcatgat tttttatttg aaccgccgcc gttttgccct tcatacaaac gcacaatatc
    10621 aaacaaattt aaaacgtccc aaccttcggt taatctaccc accgtaaaaa tagcacgaat
    10681 cggattatca accgcttcca gattgtttag caatttttcc gtttcgctgt cggttttttc
    10741 ggttttgctt ttgttggttt cggagttggt aataatgata ttgtgttttt gataattttg
    10801 ttttatccaa tttgctaaat gtgcaaactc aaagccattt tcctgcataa attttagggc
    10861 ttgttcggtg cgggttttgc cttgttcgtt ggcgttatcg ctatcgttta agctcattga
    10921 aaatgtcttt aaaaacgaaa aatcatctgc ttgcacattt tctacccaat ttaaaaatgc
    10981 caaataatcc gcttttgatt catcaaccgt cttgcttcta aacaacatca caggcttgaa
    11041 attggcaatg ccatatttca aagcgatttg atggcgatac caagcaaaca ataaagcgtg
    11101 caacacccgc tctttcttgc tcagtgtact ggataccaaa ttgatttctt tggtataacc
    11161 tttttgcaaa aattctttta aaccaaattt tgtgatgatt ttatcagcgt atttttgttg
    11221 cacgtcagct ttttcaggtg gcgtggcggt aaattccaac agcacatttt ggccgggatt
    11281 gccattttta ttgagcaaca attccaaaac catatgctcc cagcctttgc gttcaatttc
    11341 ggcatggcta gtgcggtcgt tcatttcctt ttctaaatct aattcgcctt gttttttgcc
    11401 tttggtttgc gcgtttaaat ggtgtgcttc atcgcccagc attacaaggt tcaaatcgtg
    11461 caaatctgcc aatgtggttt ggttttctcg ctcggtgtga atatcgttat acagtttttg
    11521 aatgctggta aatttaattt caatgccgtc tgaatgtggg ctgaacgttt ccactttgcg
    11581 aatgggaatt accgtatcgc cctgcaaaat cttttcggta aataaaaatt ttgcgtgcgt
    11641 cgggtcaata aaattatttt ccgttttatc cacgatatta ttttgattca caaaaaacag
    11701 aaaatgccga tacccttttt caaaataata caaaatcaac gccgccatca tcatcgtttt
    11761 gccggcacct gttgccatat taaatagcaa atgcgttggc ttatttttta aatcagggaa
    11821 atcgtctagc tctgaagtgc ggtcaaaaat caaaaagttt tcaagggcgg atttttgcca
    11881 ttcaaaaaaa tcatatttta agtttttgga cgtaaactca ggcatttcaa gtaattcagg
    11941 cttgtatttt ttaaccgctt tacgttcatt ttcaatcact tcaaacaaag ttttattatt
    12001 cgccatcttt tttctccgct tgatagaacg cacggcttaa cgctttatcg tcatcattca
    12061 ggcagcctgc aaattgttca tcgtccattt cggataatga aacatacatt tgatttaaat
    12121 ccaacatttc aagcaccatt tgtttttgtt cgtctaatgg caaagattga aattcaggct
    12181 cttgaatgat ttgactaaat tcatttacgc tgacgttgta tttcaagaaa tagcgttcgc
    12241 ataaaccttc aaacagcgtt ttgatgccgt ctgaatcttc gcaagccaaa atttgttgtt
    12301 ttgcggtttc gttaaatgga gcaagttcgg cataaacaaa ctcgccacca ccttgccaat
    12361 tcacggcttt ggaaataccg ccttgctcgc cttcaatcac ttttttcaag cgttcaacgg
    12421 caagcgtttc aatatagtcc atttgttcaa taccgatgta ttggcggttc attttgtggg
    12481 caacggcggc ggttgtgcca ctaccaagat ggtagtctag gacgatgtcg ttttcgttgg
    12541 ttgtgatggt aataaaatct tggataagtt cttctggttt aggattttta aatgcctttt
    12601 caccaaaaag atttttcatc tgttcagttc cattaccact actgtattca ggtttataaa
    12661 aaatagtttt aggttttttt gtaggtaaat catctaattc aggtctttgt tttttataca
    12721 aggaaattcc atttttagat tggctaacaa taatatccgt ctgcagcctt gccttattct
    12781 ttattgaata cccccaacgc caacgccccc attgtttatc agcaataggt aaaataaaat
    12841 tgtatccaag attttcgtat ttctctttta gtttttgaat aaaatcatca ttgaatactt
    12901 ctaaatcttt attatagatt tgagaaaatt cttcatctgt aattgtacta actgtgttat
    12961 tttttaccaa aaaaggatag aacatttcag gtctatcttc tcttttatct tcggttcctg
    13021 ttgctcgcat tggagctcct tttttataaa acccaatttc atcttcttcc catttctcta
    13081 aaaaattatc ttcatctatt ggaaattcat aaaaagtaga tttatcttta tttttagcaa
    13141 agactagtgt gtattcgtga gtgcctgcaa aagcatattc atcattatta ccttttaaat
    13201 tcataatagt gggtaaattt ccgataaaat tttcaaatcc aaaaatttca tctaacaaaa
    13261 tctttaaata agcatttccg tcatcatcaa tggaaataac aataatccca tcatcagtta
    13321 ataattcttt ggcaattgat aaacgatttt tcataaatgt aagccaagta gataatttaa
    13381 agttagtgtt ataaccaaaa ctatcttgtt gtttcttttt aacaaaataa taaggcggat
    13441 caatataaat cagcttcact ttacctttaa actgcttggc aagcgaatgc aggacaatca
    13501 gattattgcc tttgataatg agattttcag ctggcgtacc gtctgaatgt cgtttgattt
    13561 cgccaaccgc ttgtttgccg tctgcggtgt ggcgagagaa ttttgagaat gcttttgcgt
    13621 caaaaagtcg atcgatttca tcaaaagcaa gcgtttgatt aaaaaagatt tcttgtcttt
    13681 ttcgggttaa tttagtgtat aattggctgg ctggctggct ggctggctgg ctggctggct
    13741 ggctggctgg ctggctggct ggctggctat tactgtgttt aaaataaatc tcttcgcttt
    13801 cttcgctgct ttgtccgcca ttcaatacgc aatctttaaa cggaaaatcc aacacaatat
    13861 ccgaactgtc tttcaaaaag cggttgccgt ccgtcaaacc aatgcggttg gcgtattttg
    13921 tgtaggaatt attgacgctg tgtttgtcca agaaaaaacg gaaatcctgc aatttaaaca
    13981 ccaaaacacc attcacttcc acaaaaaaat ggcgttttaa ctcatcattc cccaataaca
    14041 acccgataat ggacgggtcg gttttttcca ccaaatccaa caggatgttt ttggctaaaa
    14101 tggttttttc ttcgttcgcc catacttttt cgtgtggtag cagggcttgg gttaattcgg
    14161 cttgaatgtc tgtcttcatc gttataaatc ctcatcagtt attttaatgt gccattcttg
    14221 tgtaacggca aatggctttt caaataaatt ctaataaaat caattctttg atgtctttgc
    14281 tgtttccggg ggcgttcgca aagttgcgat aagcggggcg ggtgtccgag tgtgtcggaa
    14341 ttttgcggaa atatagtgga ttaacaaaaa tcagggcaag gcgacgaagc cgcagacagt
    14401 acaaatagta cggcaaggcg aggcaacgct gtactggttt ttgttaatcc actatatcaa
    14461 cacattattc aaacacagcc tgtattttta accgccgttc aggcggcgga tgccgccgtg
    14521 ttcatcaggc gcaaggcttt cagacggcat atttcctttt gatattctgc taaaccgaca
    14581 aattctccgg tttcagtgta aacgcgtacc ggcgtgtcgg aaggtaggtc ttcctcgaaa
    14641 cgcggacgtt gtccgcagcg gagcatatgg acggcataat cgtttaaaac ggtttgggga
    14701 aagtgtgaaa ccaatacgtc gcagggtagc agcaagctgt cgcgttctgt ttcgttcaaa
    14761 tttgccaagg cctcaagcgt gtggctttgg gcgatagtaa agccggcggt ttcggcacgg
    14821 cgcagggcgg tcaggtgggc gaacgtgccg atgtgtttgg cgatgtcttc gctgagggtg
    14881 cggatatagg tacctttgct gcaacgcacg cttatcacgg ctttgggcgc atcaaattcg
    14941 gcaatatcga tggcgtaaac ggtaatgtcg cgcgctttgc gttcgatgac gatgcctttg
    15001 cgcgcgtatt cgtacagcgg tttaccttcg tgcttgagcg cggaaaacat cggcggcact
    15061 tggcggatgt cgcccgttaa ggcttggcag gccgtctgaa attcggctaa ggaaatatcg
    15121 gcgcgggcag tggcaacgat ttcgccttcg gcatcgcccg tgctgctggc ttcgccgagt
    15181 ttcagcgtgg cggtgtaggc tttgtcggca tccagcaggt attgggcgaa cttggtcgct
    15241 tcgccgaagc agacgggcaa aagcccggtt gccaaagggt cgagcacgcc ggtatgcccg
    15301 gctttttcgg cacggaacaa acgccgcgct ttttgcaggg cggtgttgct ggaaaggcct
    15361 tcgggtttgt cgaggagaag aacgccgttg accgggcgtt tggtgggttt attggtcata
    15421 ttaattaggg tatattaatg ttcagaccgt ctgaaagggt tcggacggcc tgcgtgtcag
    15481 cttaattctt cattcaataa ttcacgaaca gagtgttttt catcaattag atcaacgctg
    15541 attgcatcaa agccatttcg ttttaatgag tcaaaactaa tgattcgacc atcaattaag
    15601 gggacttgtc cttcagaatt gacgaaatct tcatattcat cttcatagtc tatattgtaa
    15661 taaagatgga tcagaactcg gtaagtatac tcatccaaat cagttaacgt tctgttttgc
    15721 ttttgatttg gactatattt gtcgcaaaac atagcgttat agccatgttc cgcgccatca
    15781 aacaatacga tttcatcgcc gcttacagct tctactgcca caatttttac cggagcctta
    15841 tcagtatcgg tcatcaatcc atcaaaaggc tctccatagt aacggatgtg gaaagtatct
    15901 ctaccttggc aggataatga aaagcaacga tgcacgcctt caatttgcag gctgtcacta
    15961 tgtgcatagg cggctaaatg ttcgggaatc agattgttca ttttagcagt atcagtcttc
    16021 aaccggtttt tccgccgcca cttgatcgat aagggcggac aggttcaaac cgcgttccaa
    16081 cgattcgtcg tatttgaaat gcagctcggg gattttgaac agcttgatgc gtttggccaa
    16141 ttcgctgcgg aggtgtccgc gtgcgtgttc cagcacttct tccgtaattt cgcgcgcatc
    16201 ttggtttaaa acggtgtaga acacggtggc gtggctgtaa tcgcgggtaa cttcgacctc
    16261 gttgacggtg atgaagccgg cgcgggggtc tttcagtccg gtacggacga gttcggcaag
    16321 ctcgcgcatg atttgttctt tgacacggtc ttggcgggcg tagccgcgtt ggggttttct
    16381 catgaatatc ctcagatttc agatttggtt tcagacggca tttttgccgc cggggcggtt
    16441 gcgaaagggc gtattatagc ctaatcggcg ggattttaag gtaatacatt gataagaacg
    16501 gatatagtta ttatacttgg caacccgaac ggcgcgtgcc gtttctgtaa cgcatacatt
    16561 gtaaagaaag ccatgtccaa cgaaaaccgt acctgttcct tttgcggaaa atccaaatca
    16621 cacgtcaaac atttaattga gggcgaaaac gcctttatct gtgacgaatg cgtctcaaac
    16681 tgtattgaaa tattgcatga agacggaaat gacggcacgc ctccggaaag tgccggaggg
    16741 gagccggagg aatccggcaa gctgcccacg cccgccgaaa tcgtcgccaa tctcaacgac
    16801 cacgttatcg gtcaggaaca agccaagaaa gccttggcgg tgtcggtgta caaccattac
    16861 aaacgcctgc gccacccgaa agcggagggc aaagtcgagt tgtccaaatc caatatcctg
    16921 cttatcggcc cgaccggatc gggtaaaacc ctgctggcgc aatctttggc gcgcaaactg
    16981 gatgtgccgt tcgtgatggc agatgcaacc acgctgaccg aagcaggtta tgtcggcgaa
    17041 gatgtcgaac agattattac caaactgttg ggcaaatgtg attttgacgt tgaaaaagcc
    17101 cagcgcggca tcgtttatat cgatgaaatc gataaaattt cacgcaaaag cgacaatccg
    17161 tccatcacgc gcgacgtatc cggcgaaggc gtgcaacaag ccttgctaaa actgattgaa
    17221 ggcacggtag ccagcgttcc ccctcaaggc ggacgcaaac atccgaacca agaattcatc
    17281 aacgttgata ccaccaacat cctctttatc tgcggcggcg catttgccgg cttggaaaaa
    17341 gtcatccgcc aacgtactga aaaaggcggt atcggctttg gtgcgtccgt tcacagcaag
    17401 gacgaaaatg ccgacattac caagctgttc ggcatcgtcg aaccggaaga cttgattaaa
    17461 ttcggtctga ttccagaatt aatcggacgt ttgcctgtga ttgcaacttt agaagaattg
    17521 gacgaagacg cgctgattaa tattttgacc gagccgaaaa atgctttggt caagcagtat
    17581 caggcgttgt tcggtatgga aaatgtcgaa ctggagtttg aagaaggcgc attgcgttcc
    17641 atcgcgcgtc aggcaatgga acgcaaaacc ggcgcgcgcg gcctgaggtc catcgtcgaa
    17701 cgctgcctgc tcgatacgat gtaccgcctg cctgatttga aaggcttgaa aaaagtggtg
    17761 gtcggtaagg cagtcatcga agagggcagg gaaccggaat tggtgttcga gtcctgatgg
    17821 caaacaaaac cgcttcggaa cgttgaacca cgtttctgaa gcggtttttt gtttctgatg
    17881 ccgtctgaag ccttcccgcc ttcagacggc atttgagttt tacggattgt ctgatgtcgc
    17941 ggcaaattcc gtcagcgcgt gttccaaacg tcgcaaacct tccgccgtat cctcatcgtt
    18001 caacagcagc gaaggcgcga aacgcaatac gtccgcacct gcaaccaaaa tcatcacgcc
    18061 gtgtttcaag gcggcggcgg taatttcgga tgcgcgtccg cgataggctt cgtccaacac
    18121 gcagccgagt agcagtccca ttccgcgaac ctgtgagaac aagcctgttt tcctgcccaa
    18181 atccagcaat gccgtctgaa gtttctgccc ctggctgcgg acgttgtgca aggtttccgg
    18241 cgcgttgatg atgtcgaagg cgcggctgcc gaccgcgcac gccatcgggt tgccgccgaa
    18301 agtcgagcca tgcgtgcccg gttggaaggc ggcggcaatc gtttctgtcg ccagcatcgc
    18361 gccgatcgga aagccgcagc ccaaggcttt tgccgaactc aaaatatcgg gaacaacgcc
    18421 gtaatgttca taggcaaaca gcctgcccgt atgccccatc ccggtttgaa cttcgtccaa
    18481 aatcaacaac gcattgtgct ggtcgcacag acggcgcgcg gtttgcaaat attccgcagt
    18541 ggcgggcagg atgccgcttt cgccctgtat cggctcgatg atgaccgcgc aggtctgttc
    18601 gccgacggca gcttccagcg cggcaatatc gttgaacgga acgtgcgtaa tgccttgcgg
    18661 caggggtgca taatccttgc tgtatttcgg ctgaccgccg acggacacgg taaacagcgt
    18721 gcgtccgtga aaactgttga tacaggcgac gatttcgctt tttcctccgc cgaaacggtc
    18781 gcgggcgtat ttcctcgcca gcttcaacgc cgcctcattc gcttccgcgc ccgagttgca
    18841 gaaaaaaacc ttgtcggcaa aactgtttgc aaccaatttt tgcgccaatt cctgcgccgg
    18901 acgcgtcgta tagatattgg aaatgtgcca cagcttgtgc atctgcgcgt ttaaagcatc
    18961 ggcaagggca gggtggcagt gtcccagcgc attgacggcg ataccgcctg aaaaatcaat
    19021 atattcacgc ccttccgtat cccaaacgcg gctgcctgaa gcgcgttcgg gaatcatcgg
    19081 ggcaaaggcg aaattggggg tcagatagtt ttgcataatt gctcctgatt gcggttttgc
    19141 tcgggattat gcaccgtttt tcaaagcaga taaagggaaa ttgccgacaa tatcgggcag
    19201 cggtaaaaat aaaccgaaat attttcaatc ggatagatga aaaatcaaaa aaatttcgat
    19261 aaaatgcttg aggcgcggca gcgttttgat ttagaatctc cccaatattt ttatcttcag
    19321 acggcatttc atcatgcaag gcaaaaaatt tacaaccgct tattgctatt ggtaccacac
    19381 agacgtgcgg tacttttttg cgcttgcttg aaatccatcc catacgcaaa acaggccgct
    19441 cgaaaacggg gcggcttttt tgttaccttc acacaggtcg cccgattcgg caacacacga
    19501 cagaatcagg aaaatgacca tgaatgcaat gccccaaacc gccgcacaaa acgaaaccct
    19561 gcacatttgc agcattattg tcgatgatgc acaggatttt tcccccatgc ttctcgatgc
    19621 agggcgggac gacacactta tcagcgaatc gatgattccg caaatccgca ctgttgccgc
    19681 cctgatagcc gccgaacggc acgacttcag ccgcagcagc cccgccgagt ttaccgatgc
    19741 cgccgatttt tttgccgcac ggattctcgt attgggcgtg cgacgcttcc atctggacgt
    19801 atcgctgctt cagattttga aaaccgccaa cagccgcgcc cgccgctttg ccgaaaaaca
    19861 ccgcctgttt ttcacacccg cacaagccga attgagcctg aacaaacaca aaaaccgccg
    19921 cctgctgacc gtagaaaccg aacacgaagt cgaaaacaaa ggcaacccag tcgccaacag
    19981 cctcgcattt gtccgcaaac tgcacaccct gcccctgtaa acatcacgat ttgccggcag
    20041 aatgcttgcc aaaattcccg acggcgatac aataaaaccg attttccacc caaacaggac
    20101 acacatgaaa aaaatcatcg cctccgcgct tatcgcaaca ttcgcactcg ccgcctgcca
    20161 agacgacacg caggcgcggc tcgaacagca gcagaaacag attgaagccc tgcaacagca
    20221 gctcgcacag caggcagacg atacggttta ccaactgact cccgaagcag tcaaagacac
    20281 cattcctgcc gaagcacagg caaacggcaa taacgggcaa cccgttaccg gtaaagacgg
    20341 gcagcagtat atttacgacc aatcgacagg aagctggctg ctgcaaagcc tggtcggcgc
    20401 ggcggcaggc gcgtttatcg gcaacgcgct ggcaaacaaa ttcacacggg caggcaacca
    20461 agacagtccc gtcgcccgac gcgcgcgtgc agcctaccat cagtccgcac atcccaatgc
    20521 gcgcaccagc agggatttga acacgcgcag cctccgtgca aaacaacagg cggcgcaggc
    20581 gcagcgttac cgcccgacaa cgcgcccgcc cgccaattac cgccgccccg ctatgcgcgg
    20641 tttcggcagg aggcggtaaa cccggcgtgc caatgccgtc tgaagggctt tcagacggca
    20701 tttttgcatt tgttaggggc attgttatgt tgccgtttga ttttcagacg gcattttgtt
    20761 tcccagcgtt tgatgtcggg atggcaattc tgatttacgt gcgttggcag ggcggcattg
    20821 tcatgccgcc gttgggtttt cagacggcat catatcgaac agtcaagccg tagggcgagc
    20881 attcttgccc accgttgcaa gggcgggatt gggtgggcag gaatgcccac cctgcatctg
    20941 aaacccttcc gtgtcattcc cgtgcaggaa ggaatctaga tatttgaatt tacggaaatt
    21001 ttcaatcgtt gccaaagcgg aaaagggttt ttattacagc ctaatccttt gggtatcaaa
    21061 gccgggcggt gttgaaacat tgcaaaaatg ccgtctgaaa cccgaaatcg gtttcagacg
    21121 gcattggttt atgcggcagg ttggggctta gcggtctttg taacggcgtt cgcctttgcc
    21181 tgcaaaagaa tcgccggctt tacgtttgcc tttgaagcct tcgccggttt ttttgaaacc
    21241 gtctttctta aagccttctt tcttgaaacc ttcgccgcgc gttttgccgc cgaagccttc
    21301 tttgcccggt ttatgatcgc cgcgccaacc gccggattta cgatcgcccc agccgccttt
    21361 gcctttcggc ttgccgcctg cggatttgcg tttgcgggtc ggttccatgc cctcgatggt
    21421 cagttcgggc agttttcggt taatgtattt ttcgattttg tggactttga cgtattcgtt
    21481 cacttcggca aacgtaatcg caatacccgt gcggcctgcg cggccggtgc gcccgatgcg
    21541 gtggacgtag tcttccgcct gtttcggcag gtcgtagttg ataacgtggg taatggtcgg
    21601 tacgtcgata ccgcgtgcgg ctacatcggt ggcaaccaaa attttgcagc ggcctttgcg
    21661 caagtccatc agcgtgcggt tgcgccagcc ttgcggcata tcgccgtgca ggcagttggc
    21721 ggcgaaacct ttttcgtaca attcatccgc gatgacttcg gtcatggctt tggtggacgt
    21781 gaaaatcacg cattggtcga tgttggcatc acgcaggatg tggtcgagca ggcggttttt
    21841 gtggcgcata tcgtcgcagt acagcagttg ttcttcgatt ttgccttggt cgtccacgcg
    21901 ttcgacttcg atgatttcag ggtctttggt cagtttgcgt gccagtttgc cgaccgcgcc
    21961 gtcccaagtg gcggagaaca ataaagtctg acggtcgctc ggcgttgctt ccacgatggt
    22021 ttcgatgtcg tcgataaagc ccatatccaa catacggtcg gcttcgtcca gaatcagcac
    22081 ttccaaacgt tcaaaatcga ctttgccgct ttgcatcaag tccatcaggc ggcccggcgt
    22141 ggcgacaatc agatcgaccg gtttgctcag ggcgcgggtt tggtagccga acgatgcgcc
    22201 gccgacgatg ctgacggtac ggaaccaacg catatttttg gcatacgcca gcgcgttttt
    22261 ctcgacttga gccgccaatt cgcgggtcgg ggtcaacacc aaagcacgcg ggcctttgcc
    22321 cggtttttcg ctgcgtttgg tcagtcgctg caaagtcggt aacagaaagg cggcggtttt
    22381 gccggagccg gtttgcgccg aagccatgat gtcgcggcct tccaaagcaa acggaatcgc
    22441 ctgcgcctga atcggcgtcg ggctttcgta accctcgctg ctgacggcgg acaaaatgtt
    22501 tttatcgagg ttcaaatcgg caaatttaat agacatggct atccttggaa gacaacaacg
    22561 cccgcgtccg aaaggttcag acggcctgaa gcagggaagg gtaacgatac ggggaatgtg
    22621 aagttacagg gttgtcgcaa gcggcttgct tgtggtttac atcgacggca acctgcacag
    22681 atacgctttg taaaatcaaa gcaacagaag aaacgcgccc gtaagggcgg tttaggttgg
    22741 aaggcgatgg cttcgtatga caagataagc ggcggatggt atagggtttg cccgaaacgg
    22801 tcaagcaatc gggcgttaat tacagtaaaa ttgccgtttt ccactttatt ccgcctgaaa
    22861 ccaatgaccg acatcacacc gtttgccaac cgcttgggca aaaacatcaa acaccttatg
    22921 aaatgggcga aacgcaacgg catcgaagcc tggcgcattt acgaccgcga cattccccaa
    22981 ttcccttttg ccgccgatgt ttacggtgat cgaatccacc ttcaggaata cgataccggc
    23041 tggctgatgc agcccgaaga atacgaagcg tggcttgccg aagtattgga agccgtcgtt
    23101 ttcgttaccg gttttgcgcc cgaacaaatc cacctcaaac gccgcgaacg tcaaaaaggt
    23161 ttgcagcaat acgagaaaac cggcaaaacc ggcgatgatt ttgtcatcac cgaaaacggc
    23221 cgcaagtttt gggtcaacct cgacaaatac ctcgataccg gccttttcct cgaccaccgc
    23281 aacacgcgca aaaaagtcgg cgaaaccgca gcgggcaaac gcttcctcaa cctgttttcc
    23341 tacacaggca gcttcaccgt ctacgccgcc accggcggcg cggcatccag tgaaaccgtc
    23401 gatttgtcca acacttatct cgattgggcg aagcgcaatt ttgaattgaa cggcatcgat
    23461 acggaacggc acaaaatcgt ccgcgccgac gtgttccaat atcttcagac ggcatatggg
    23521 gaaggcaggc agttcgacct cattgtgatg gatccgccca gtttttccaa cagcaagaaa
    23581 atgtccgaca tcctcgacat tcagcgcgat cacaaaaagc tgattgacgg cgcggtgaag
    23641 ctgctcgctt cagacggcat tttgtacttt tccaacaacc tgcgcagctt cgtcttagac
    23701 gacttggtat cggaacaata cgccgtcaaa gacatttcca aacaatccgt tcccgaagat
    23761 ttccgcaaca aaaaaatcca tcggtgctgg gaaatccgcc acaaatctta agggtgcgaa
    23821 aaagccgccg gaatcttccg gcggcttttt cgattgcgct ttatcaggcg atagaggctt
    23881 cgacaaaggc ggttagctgg ccttttgcca atgcgccgac tttggtggcg acgacttcgc
    23941 cgtttttaaa caccatcagg gtcggaatgc cgcgcacgcc gaaacgggac ggggtggctt
    24001 cgttgtcgtc gatgttgatt ttgaccactt tcagacggcc ttcaaattcg gcggcaatgt
    24061 cgtccaaaat cggggcaatc attttgcagg ggccgcacca cggagcccaa aagtccagca
    24121 ggacggggat atctgcattt aaaacgtctt tttcaaaagc ggcatcgctg gtgtgtacaa
    24181 tcaattcgct gctcattatt tttccttttc ggttgggatt ggcacgatag cgttcaggat
    24241 agggcttggg cataaaaatt tcaagtgggc gggcgggata atagtttttc tgtaccgccc
    24301 caatcgcctt tgcaaattct aaaaaacggt tcggacagta ttttatttct gaaaattact
    24361 tttattatca aaatgtaagg aatacgtttg aggctgtaca gaagcgcgcg ggcggtgtat
    24421 gctccgtttg aattgattct taaagtatga atgttcaaaa ccaaacccga aaccctgtaa
    24481 aaggaacact gcgatgactg atttttccgt ttgggagacc gccccctttg gtgcaaccgt
    24541 cgatcatatc ctgcaacgct accacaatgt ccaccgcgca caatttgaag aattggtgcc
    24601 gctggcgcag aaagtggccc aagtccatgc cgataccttt ccggcagaaa ttgccgggct
    24661 gcttgcctat atgcagaacg aactgctgat gcatatgatg aaggaggagc ggatgctgtt
    24721 tccgatgatt aatcagggta tcggtcgggg tgcggcaatg cccatcagtg tgatgatgca
    24781 cgaacatgaa gaacacgacc gcgccatcgc acggctcaaa gagctgaccg atgatttcca
    24841 agtgcccgaa ggagcctgcg gaagctggac gcggctttac gctttggcca aagaaatggt
    24901 ggaggatttg aacgaccaca ttcatttgga aaacgatatt ctgtttgccc gcgtactcga
    24961 ttcttaaggg ataggagtcg gatatttcaa tgccgtctga aacacggttt tccgcttcag
    25021 acggcatttt catgtttcag ccaagcaatt cgggcggaat gcggcacacg ggaatcgggt
    25081 tgattttgtg ctgcatggcg cggtgaagcc gcgtatagat ttccagaact tcgcgccgcc
    25141 gcccttcaaa atcttcgggt ttgcgcgtgc cgtacacacc cattgcccac tccagttcgg
    25201 gatagcttgc gcccatctgt tcctcgtcgg tgcgttccgt atcccacagg ccgtcggtcg
    25261 gcggggcttt ttgaatcgcc tcgtccacgc ccaatgcttc ggcaagccgg taaacctgcg
    25321 ttttggtcag gtcggcaatc gggctgatgt ccacgccgcc gtcgccgtat ttagtaaaaa
    25381 agcccacgcc gaaatcttcg attttattac ctgtccccgt aaccagcagt ccgtgtatct
    25441 gcccgtagta gtacagggtc agcatacgca ggcggcttct ggcgttggcg agggaaagcg
    25501 gctgattgtc aaatgccgtc tgatgaacgc cgacggtttg ttcaaaggtc tggaaggtgt
    25561 cggtcagatc gaccgtttgc gcgcttacat tggcatattg cccttgcaga ttgcggatgt
    25621 gcagccttgc ccgctcaagc tggtcggggt gttggcgtat cggcatatcc agaagcagcg
    25681 tggtgcggcc ggtgcgggcg gcgagtgcgg agacgacggc ggaatcgatg ccgccggaaa
    25741 cgcccacgac gaaccctttt gcatttgcct gagcggcgta tttgtccaac catcggacaa
    25801 tatgtgtgat aactgcctgc gtatccattt ttcctcctgt cgaaaatatc cgcatcatag
    25861 cctgccggcg gttcgatttg aatagattgc aggcaggttg atgaatattt ttcccattcc
    25921 ccgcttaaaa aagtcatacg ggcaaaggcg ggcggcaaga tggcgcgtgt tataatattg
    25981 ttgacaattt aggcggcccg atgccgcctt gggcttcaaa acatattcat attttttatt
    26041 atttgattga ttcgatgtct gatttttttc attctgacgt gttgtccgtt tccgagttga
    26101 acgcatttgc caaaagtatt ttggaaaacc accttgccgg tttgtggatt gccggcgaag
    26161 tgtccaacct gacccgtgcc gccagcgggc attattattt ctcgctcaaa gacagccgcg
    26221 cgcaggtgcg ttgcgcgatg ttcaaaggtg cggcggcgcg tttggcgaag cctttgaaag
    26281 aaggcgatca tatcgaggta tcaggaaaaa tcagtattta cgaggcgcgg ggcgaatttc
    26341 aaattaccgt aaacgaagta cggctcaaag gtttggggca gctttacgaa gcctatgagc
    26401 ggttgaaggc gcagttgcag gcggaaggcg cgtttgcggc ggaacgcaaa aaacctctgc
    26461 ctgcccgacc gcaatgtatc ggcatcgtaa ccagtttggc ggcggcggca ttgcgcgatg
    26521 ttgtaaccac cttgaaacgc cgcgcgcccg aaattcccgt catcgtttat ccgaccgtcg
    26581 ttcaaggcac aggcagcgag ttgcaaatcg cacaagtgat taaaaccgca tcgcaacgcg
    26641 ccgaatgcga tgtgttgatt gtctgtcgcg gcggcggcag cattgaagat ttgtgggcgt
    26701 ttaacgaaga accggtcgta cgcgcaatcg aagcctgcac gattccggtc gtcagcggcg
    26761 taggccacga aaccgatttc acgcttgccg attttgttgc cgacgtacgc gcaccgacac
    26821 cgaccggtgc ggcggagctg gtcagcccca accgccaaga atcgctacat cgtctcgctc
    26881 aggcgcaagg ccgtctgaaa accgttttgg aacaacgcta tttcgatgcc agccaaaaac
    26941 tcgactggct ggcgcggcaa atccgtcatc cgcgccaaaa actcgacgaa cagcgcacct
    27001 acatccacaa actggcgcaa acgctgtctt actcgatgac gcaaaacctc cgcgcccacg
    27061 ccgcccgttt cgaacgccaa acccaagccc tgcaacatta ccgtcctgat atttccgttt
    27121 gcaaaaaaaa catcgaccgc tttcagacgg cattgcccgc cgccttcagc caattgctcg
    27181 cccgccgccg ccaaagcctg agcgcccaag cagcattgct cgaagccgtt tcgccgcagc
    27241 acattctgga gcgcggcttt tccgtcgtca aaaacacacg cggacaagtc atccgcaacg
    27301 ccgatgtttt gaagcagggg cagaagctgc acattatctt cgccgacggc gaaaccgacg
    27361 tgcgcgtaag caaagaacaa gggcagcagg atttgttcga ttgcatttaa acccaatcgg
    27421 aaaaaccgct ctccctccgc cgtcattccc gcgaaagcgg gagtccggaa gcggaaagcc
    27481 gccggggttt atcagaaatg actgaaactc aaaaaac
//
LOCUS       DAXHRI010000010        26295 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXHRI010000010 DAXHRI010000000
VERSION     DAXHRI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.010"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            182..538
                     /locus_tag="ACFM75_000305"
     CDS             182..538
                     /locus_tag="ACFM75_000305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC6254905.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            529..1011
                     /locus_tag="ACFM75_000306"
     CDS             529..1011
                     /locus_tag="ACFM75_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC6254906.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1024..1617
                     /locus_tag="ACFM75_000307"
     CDS             1024..1617
                     /locus_tag="ACFM75_000307"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC6254907.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIESREITPRIVREENYGGQ"
     gene            1607..2863
                     /gene="nuoD"
                     /locus_tag="ACFM75_000308"
     CDS             1607..2863
                     /gene="nuoD"
                     /locus_tag="ACFM75_000308"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC6254908.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2863..3336
                     /gene="nuoE"
                     /locus_tag="ACFM75_000309"
     CDS             2863..3336
                     /gene="nuoE"
                     /locus_tag="ACFM75_000309"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC6254909.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGVNAADYLKQK
                     LGIGFGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEVIEKKLAELE"
     gene            3726..5027
                     /gene="nuoF"
                     /locus_tag="ACFM75_000310"
     CDS             3726..5027
                     /gene="nuoF"
                     /locus_tag="ACFM75_000310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC6254910.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLGEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFKLFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5027..5464
                     /locus_tag="ACFM75_000311"
     CDS             5027..5464
                     /locus_tag="ACFM75_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254911.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            5754..8015
                     /gene="nuoG"
                     /locus_tag="ACFM75_000312"
     CDS             5754..8015
                     /gene="nuoG"
                     /locus_tag="ACFM75_000312"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC6254912.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVHSAIKCIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAVHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQIAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8018..9094
                     /gene="nuoH"
                     /locus_tag="ACFM75_000313"
     CDS             8018..9094
                     /gene="nuoH"
                     /locus_tag="ACFM75_000313"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC6254913.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            9175..9654
                     /gene="nuoI"
                     /locus_tag="ACFM75_000314"
     CDS             9175..9654
                     /gene="nuoI"
                     /locus_tag="ACFM75_000314"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC6254914.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            9666..10337
                     /locus_tag="ACFM75_000315"
     CDS             9666..10337
                     /locus_tag="ACFM75_000315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC6254915.1"
                     /translation="MTFSAILFYILAAIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            10334..10639
                     /gene="nuoK"
                     /locus_tag="ACFM75_000316"
     CDS             10334..10639
                     /gene="nuoK"
                     /locus_tag="ACFM75_000316"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC6254916.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            10673..11248
                     /gene="fic"
                     /locus_tag="ACFM75_000317"
     CDS             10673..11248
                     /gene="fic"
                     /locus_tag="ACFM75_000317"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6254917.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            11372..11713
                     /locus_tag="ACFM75_000318"
     CDS             11372..11713
                     /locus_tag="ACFM75_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254918.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            11762..13786
                     /gene="nuoL"
                     /locus_tag="ACFM75_000319"
     CDS             11762..13786
                     /gene="nuoL"
                     /locus_tag="ACFM75_000319"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC6254919.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQTATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPVTWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            13875..15371
                     /locus_tag="ACFM75_000320"
     CDS             13875..15371
                     /locus_tag="ACFM75_000320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC6254920.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKM"
     gene            15381..16826
                     /gene="nuoN"
                     /locus_tag="ACFM75_000321"
     CDS             15381..16826
                     /gene="nuoN"
                     /locus_tag="ACFM75_000321"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC6254921.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVIMSLIGAFYYLRVVKVMYFDESGRAHPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            16938..17237
                     /locus_tag="ACFM75_000322"
     CDS             16938..17237
                     /locus_tag="ACFM75_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC6254922.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(17346..18242)
                     /locus_tag="ACFM75_000323"
     CDS             complement(17346..18242)
                     /locus_tag="ACFM75_000323"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC6254923.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVGQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEANALLTGDALQTQAFDVLARPSGLPASRQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(18226..18537)
                     /locus_tag="ACFM75_000324"
     CDS             complement(18226..18537)
                     /locus_tag="ACFM75_000324"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC6254924.1"
                     /translation="MPSEAGFGFQTASVWQKCYNPLFTGTPETMKKNAPKSFEEALSR
                     LESLTQAMQGEMPLEDALAAYQEGNELVRYCQTKLAQVEQKLQVLDADGPKELNLESD
                     E"
     gene            complement(18564..19487)
                     /gene="rsgA"
                     /locus_tag="ACFM75_000325"
     CDS             complement(18564..19487)
                     /gene="rsgA"
                     /locus_tag="ACFM75_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC6254925.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(19492..21348)
                     /locus_tag="ACFM75_000326"
     CDS             complement(19492..21348)
                     /locus_tag="ACFM75_000326"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6254926.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(21374..21511)
                     /locus_tag="ACFM75_000327"
     CDS             complement(21374..21511)
                     /locus_tag="ACFM75_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254927.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(21511..22095)
                     /gene="ruvA"
                     /locus_tag="ACFM75_000328"
     CDS             complement(21511..22095)
                     /gene="ruvA"
                     /locus_tag="ACFM75_000328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC6254928.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAAAETEDIVSTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(22177..22503)
                     /locus_tag="ACFM75_000329"
     CDS             complement(22177..22503)
                     /locus_tag="ACFM75_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6254929.1"
                     /translation="MNIKHLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(22565..23284)
                     /locus_tag="ACFM75_000330"
     CDS             complement(22565..23284)
                     /locus_tag="ACFM75_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC6254930.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSALV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNLASSASNPNLSVEKRHYEYVVKMGPFSSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(23412..23876)
                     /gene="trmL"
                     /locus_tag="ACFM75_000331"
     CDS             complement(23412..23876)
                     /gene="trmL"
                     /locus_tag="ACFM75_000331"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC6254931.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(23941..24666)
                     /locus_tag="ACFM75_000332"
     CDS             complement(23941..24666)
                     /locus_tag="ACFM75_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6254932.1"
                     /translation="MDFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCRDDLAAYR
                     TDAANSCPLCFMHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIHALKHLADLSM
                     VQPLADLMMQNPPDRLADECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWRTL
                     PLHTVFRRHRPPQSTLKGGERLKNIKNAFEIREPLPKGCNILLIDDVFTTGATLDELA
                     KMLKKSGANRICCWTLARTPMKK"
     gene            24665..25408
                     /gene="bioH"
                     /locus_tag="ACFM75_000333"
     CDS             24665..25408
                     /gene="bioH"
                     /locus_tag="ACFM75_000333"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC6254933.1"
                     /translation="MPDAVKKVYLIHGWGANRHVFDDLMPRLPATWAVSAVDLPGHGD
                     APFVQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLAR
                     CGTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRRLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            25410..26195
                     /locus_tag="ACFM75_000334"
     CDS             25410..26195
                     /locus_tag="ACFM75_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6254934.1"
                     /translation="MNHQDARWQVHRHLAEHTDQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAARKGGFWQRFAGKGVVQHCQSPTAPLPEACADM
                     LWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSVM
                     FPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAAR
                     SCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         5686 a         6718 c         7366 g         6525 t
ORIGIN      
        1 cgaattacgg tgtaaaaatt tatatgacct tataaaatca aataagaatc gttatcataa
       61 catgattgta tttattgggt ttttttgggc gttttgccga tatttacctt ttaatggttt
      121 ttgaaattcg ctaaaatacg aaattattgt agaaattttg ttaacggatt tgggtgtaac
      181 catgttgtcc gcttactttc ccgtctttgt ctttatcctc gtcggcctcg cggccggcgt
      241 gctgtttatc ctgctcggca cgattttagg cccgaaacgc cactacgccg aaaaagacgc
      301 gccttacgaa tgcggttttg aagcctttga aaacgcaagg atgaagttcg acgtgcgcta
      361 ttacctcgtc gccatcctct tcatcctgtt tgatttggag gtcgcgttca tgttgccgtg
      421 ggcagttgtg ttcaaagatt tgggcgcgta cggcttctgg tctatgctgg tgtttatcgt
      481 cgtcctgacg gtaggctttg tttacgaatg gaaaaaaggt gcgctggaat gggaatagaa
      541 ggcgttttga aaaaaggttt catcaccacc agcgcggata cggtgttgaa ctatatgcgt
      601 accggttcgc tgtggccggt tactttcggc ttggcctgct gcgccgtgga aatgatgcac
      661 gcgggtatgg cgcgttatga ccttgaccgt ttcggcatca ttttccgtcc gtctccccgt
      721 caggccgacc tgatgattgt ggcaggtaca ctgaccaaca aaatggcgcc cgccctgcgc
      781 cgcgtatacg accagctcgc cgagccgcgc tgggtattgt ctatgggctc gtgtgccaac
      841 ggcggcggct attatcacta ttcttattcc gttgtgcgcg gtgccgaccg cgtcgtgccg
      901 gtagacgttt atgtgccagg ttgtccgccg actgcggaag ccctgattta tggcctgatt
      961 cagctccaac aaaaaatcaa gcgcacttcc accatcgcgc gtgacgaata aggagaggac
     1021 gatatggcaa gcattcaaaa cttatatgag accgtcgtcg gcgtgcttgg cgatcaggca
     1081 agcaaagtca tttcagcttt gggcgagatt accgtcgagt gtctgcccga acactatatt
     1141 tcagtcatga ccgcattgcg cgatcatgaa gagctgcatt tcgagcttct ggtcgatttg
     1201 tgcggtgtcg attacagcac ttacaaaaac gaagtatggc agggcaaacg ctttgccgtc
     1261 gtcagtcagt tgctttccgt taaaaacaat caacgcatcc gcgtacgcgt ctgggtttca
     1321 gacgacgact tccctgtagt cgaatccgta gtcgatattt acaacagcgc ggattggtac
     1381 gaacgcgaag ccttcgatat gtacggcatc atgttcaaca accacccgga cttgcgccgc
     1441 atcctgaccg attacggctt tgtcggacat ccgttccgca aagacttccc gatttccggc
     1501 tatgtggaaa tgcgttacga cgaagagcaa aaacgcgtga tttaccaacc tgttaccatt
     1561 gagtcgcgcg agatcacgcc gcgtatcgtc cgtgaggaga actacggtgg ccaataaatt
     1621 aagaaactac accatcaact tcggcccgca acaccctgcg gcgcacggcg tattgcgcat
     1681 gatcttggag ctggacggcg aacaaatcgt ccgtgccgac ccgcatatcg gcctcctgca
     1741 ccgaggtact gaaaaactgg ctgaaaccaa aacctatctg caagccctgc cttatatgga
     1801 ccgtttggac tacgtttcca tgatggtcaa cgagcaggcg tattgtttgg cggtagaaaa
     1861 gctcgccgga attgacgtgc ctatccgcgc ccagtatatc cgtgtgatgt ttgccgaagt
     1921 aacgcgcatc ctcaatcact tgatgggcat cggttcgcat gccttcgaca tcggcgcgat
     1981 gaccgccatc ctctacgcct tccgcgaccg cgaagagctg atggacttgt acgaagccgt
     2041 gtccggcgca cgtatgcatg cagcctactt ccgtcccggc ggcgtttacc gcgacctgcc
     2101 cgacttcatg cctaaatacg agagcagcaa attccgcaat gccaaagtat tgaagcagct
     2161 caacgaatcc cgcgaaggca ccatgctcga ctttatcgat gccttctgcg aacgcttccc
     2221 caaaaatatc gacacactcg aaaccctcct gaccgacaac cgcatctgga aacagcgtac
     2281 cgtcggcatc ggcgttgtct ctcccgagcg tgccatgcaa aaaggcttta ccggcgtgat
     2341 gttgcgcggt tcgggcgtgg aatgggacgt gcgtaagaca cagccttacg aagtgtacga
     2401 caaaatggat ttcgacatcc ctgtcggcgt gaacggcgac tgctacgacc gctacctctg
     2461 ccgtatggaa gaaatgcgtc aatccgtacg catcatcaaa caatgttccg agtggttgcg
     2521 tgtcaatccg ggtccggtca ttaccacaaa ccacaaattc gctccgccca aacgtaccga
     2581 aatgaaaaca ggtatggaag acctgattca ccatttcaaa ctctttaccg agggtatgca
     2641 cgttcccgag ggtgagacct acaccgctgt cgaacatccg aaaggcgagt tcggcgttta
     2701 catcatttca gacggcgcaa ataaacccta ccgcctgaaa atccgcgcac ccggcttcgc
     2761 ccacctgcaa ggcatggacg aaatggcaaa aggccacatg ctggccgacg tcgttgccat
     2821 cattggtacg caggacatcg tattcgggga ggttgaccga taatgttatc cgcagaatct
     2881 ttaaaacaaa tcgacatcga gttggcaaaa tatcctgccg accaacgccg ctccgcgatt
     2941 atgggcgcat tgcgtatcgc ccaaaccgaa aaaggctggc tcgctcccga gaccatcgcc
     3001 tttgtcgcag actatatcgg catctcgcct gcacaagcct acgaagtcgc taccttctac
     3061 aatatgtacg accttgagcc tgtcggcaaa tacaaactga ccgtttgtac caacctgccc
     3121 tgcgccctgc gcggcggcgt gaatgcagcc gattatttga aacaaaaact cggtatcggc
     3181 ttcggcgaaa ccacccctga cggcaagttt acccttgtcg aaggcgaatg catgggcgca
     3241 tgcggcgacg ctcccgttat gctggtcaac aaccacagca tgtgcagctt tatgaccgaa
     3301 gaagtgattg agaagaaact ggcggagttg gagtaggtcg tctgaaacga cgatttaaac
     3361 gtaggtcgga tacttgtatc cgacagagtg ggtaaaaagg caaaatgtcg gatttaagaa
     3421 tccgccctac tgaaataccg aaatgccgtc attcccgcgc aggcgggaat ccaccggtaa
     3481 gattcggttt ctgaatttaa taagacattg cttaccattg aggatggatt cccgcctgcg
     3541 cgggaatgac gacagacaag caagtggtcg agatccaaca aaaacgatta aaggtcgtct
     3601 gaaaatatcg atttgataaa ctagatttta tttcagacga cgttacaagc cggtacacaa
     3661 agacatctta aggtcgtctg aaacagcggc cgcaaccgat acgaaaacaa acaggcacac
     3721 caaaaatggc tatttaccaa tcaggcgtga tttttgacca agtggatacc gccaatcccg
     3781 attgctggac attgggcgaa tacgtcaaac gtggcggtta taccgccctg cgtaaaatcc
     3841 tgtccgaaaa tatctcgcaa accgatgtaa ttgacgaagt caaaacctcc ggcttgcgcg
     3901 gtcgtggtgg tgcgggcttc ccgactggtt tgaaatggag ctttatgccc cgttccttcc
     3961 cgggcgaaaa atatgtggtt tgtaacaccg acgaaggcga accgggtaca tttaaagacc
     4021 gtgacatcat catgttcaat cctcatgccc tgattgaagg catgattatc gccggttacg
     4081 cgatgggtgc gaaagccggc tacaactata tccacggcga aatttttgaa ggctaccaac
     4141 gttttgaagc cgctttggag caggcgcgtg ccgcaggctt tttgggtaaa aatattttgg
     4201 gttcggattt tgaattcaaa ctcttcgccc accacggtta cggcgcatat atttgcggcg
     4261 aagaaaccgc attgctcgaa tcgttggaag gcaaaaaagg ccagcctcgc tttaaaccac
     4321 cattccctgc ttcgttcggc ctgtacggca aaccgactac catcaacaat accgaaacgt
     4381 tctcctccgt tccattcatt atccgtgacg gcggacaggc gtttgccgat aaaggtattc
     4441 cgaatgcagg cggtaccaaa ttattctgta tttccggcca tgtcgagcgt ccgggcaact
     4501 atgaagtgcc attgggcacg ccgtttgccg aagtcctgaa aatggcaggc ggtatgcgcg
     4561 gcggcaaaaa actcaaggcc gttattcctg gcggttcgtc cgcgcccgta ttgcctgccg
     4621 acatcatgat gcagacaaat atggactacg actcgatttc caaagccggc tccatgctcg
     4681 gttccggcgc gattatcgtg atggacgaag atgtgtgcat ggtcaaagcc cttgaacggt
     4741 taagctactt ctactacgac gaatcctgcg gccaatgcac cccctgccgc gaaggcactg
     4801 gctggctcta ccgcatcgtc caccgcatcg tagaaggcaa aggccgcatg gaagacttgg
     4861 atttgctgga ttccgtcggc aaccaaatgg caggccgcac catctgcgcc cttgccgatg
     4921 ccgccgtttt ccctgttcgc agctttacca agcatttccg tgatgagttt gtgcattaca
     4981 tcgaacacgg cgggccgatg aaagagcata agtggggagg gtggtaatgg tggaagctaa
     5041 aatttttatt ctatacggtg cagccaacaa aggtaagagt acgacactca atacgctttt
     5101 taatcagatt tgttggaaat tttctaaatt tctagtcttt tttgaaagac atggaaatgg
     5161 cttagatttt gttgcagtat ttgatcatga aggtcagaga attggttttt attcatctgg
     5221 tgataatgaa tacgaggtta ggagaaattt atacaaactt tattcgcata attgtgattt
     5281 tatttttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat gttatgcaga
     5341 gttattgcat ggcgatgtaa atataattaa ttggtgtgaa aagtttgagc ctacagatga
     5401 agacaatgag cgtgctgtta aagagttatt taagtcattt aaaaatataa taaatgagtt
     5461 atagttttag ttggttttat attggttaaa agcaaaatgc taaaaattta actttgccgt
     5521 cattcccgcg taggcgggaa tccatagtgg aatttacaga acccgatatt tgaaaagcag
     5581 ttgccgaaat tcaaaaaatg gattcccgcc tgtgcgggaa tgacggcaag gtggcagtaa
     5641 acgttttaaa cagtattgat tgtcaatgaa actcaaaagg ccgtctgaaa cccatttttc
     5701 agacgacctc cataaaagat tatttatcaa atacccgtaa ctaggaacga accatgttac
     5761 aaatcgaaat cgacggcaaa caagtatctg tggagcaggg cgcgacggta attgaagccg
     5821 cgcacaagct cggtacttat attccgcatt tctgttacca caaaaaactt tccatcgccg
     5881 ccaactgccg tatgtgtctg gtgaacgtag aaaaagcccc aaaacccctg cctgcctgtg
     5941 ccacgccggt tacagacggc atgattgtgc gtacgcattc ggcaaaagcc cgagaggcgc
     6001 aggaaggcgt gatggagttc ctgctcatca accatccgct tgattgtccg acctgcgacc
     6061 aaggcggcga atgccagttg caggatttgg cggtgggcta cggaaaaacc accagccgct
     6121 acaccgaaga aaaacgttcc gtcgtcggca aagacatggg gcctttggtt tccgccgagg
     6181 aaatgagccg ctgtatccac tgcacccgct gcgttcgttt cactgaagaa atcgccggtt
     6241 tgcaggaaat tgcgatggtg aatcgcggcg aacactccga aatcatgccc tttatcggca
     6301 aaacggtgga aaccgagctg tcgggcaacg tcatcgattt gtgtcctgtc ggcgcattga
     6361 ccagcaaacc gttccgcttc aacgcgcgta cttgggaatt gaaccgccgt aaatccgttt
     6421 ccgctcatga cgctttgggc agcaacctga ttgtacaaac caaagaccat accgtccgcc
     6481 gcgtgttgcc gctggaaaac gaagccatca acgaatgctg gctgtccgac cgcgaccgtt
     6541 ttgcctatga aggcctgtat cacgaaagcc gtttgaaaaa cccgaaaatc aaacagggcg
     6601 gcgagtggat ggacgtggat tggaaaaccg cgttggaata tgtccacagc gcaattaaat
     6661 gtatcgccaa agacggcaac caaaatcaag tcggcgtttg ggccaatccg atgaatacgg
     6721 ttgaagagct gtatttggcc aaaaagcttg ccgacggctt gggtgttaaa aactttgcca
     6781 cccgtctgcg ccaacaagac aaacgtcttt cagacggcct taaaggtgcg caatggttgg
     6841 gacaaagcat tgaatcgctg gcggacaacg atgccgtatt ggtagtcggt gcgaacctgc
     6901 gcaaagaaca gccgcttctg accgcccgcc tgcgccgcgc cgctaaagac cgcatggctt
     6961 tgagcgtatt ggcaagcagt aaagaagaat tgtttatgcc gcttctgtct caagaagccg
     7021 tacatcccga cgagtgggca ggtcgtctga aaaacctgtc tgccgatgcg gaacacgccg
     7081 ttaccgccag cctgaaaaac gctgaaaaag cagcggtgat tttgggcgcg gaagtgcaaa
     7141 accatcctga ttacgccgcc atttatgccg ccgcgcaaga gctggcggac gcgaccggcg
     7201 cagtgctggg tattttgccg caagccgcca acagtgttgg tgcggatgtc ttgaatgtaa
     7261 actccggcga gagcgttgcc gaaatggcaa acgcgccgaa acaggcagtc ttgttgctca
     7321 acgtcgagcc ggaaattgat acggttgacg gtgcaaaagc cgtagccgcg ttgaaacagg
     7381 caaaaagcgt gatggcgttt acgccgtttg tcagcgaaac gctgctggac gtgtgcgacg
     7441 tgttgttgcc gattgcaccg tttaccgaaa cctcaggcag cttcatcaat atggaaggcc
     7501 gtctgcaatc tttccacggc gtggtacaag gcttcggcga ttcgcgtccg atgtggaaag
     7561 tattgcgcgt attgggcaat ctgtttgacc tgaaaggttt tgaatatcac gataccgctg
     7621 cgattctgaa agacgcgctg gatgcagaaa gcctgccgtc caaactggac aaccgtagca
     7681 catggacagg ggagggcgtt cagacggcct cagaccgcct tgtccgtgtc ggcggcgtcg
     7741 gtatttatca cactgattct atcgtgcgcc gttccgcacc gttgcaagaa accagccatg
     7801 ccgccgtgcc tgccgcgcgt gtaaatccga acactttggc acgcttgggc ctgcaagacg
     7861 gacaaattgc tgtcgccaaa caaaacggcg caagcgtatc ggttgccgtc aaagccgatg
     7921 ccggtttgcc tgaaaacgtg gtgcatctgc cgctgcatac cgaaaatgcc gcgctgggtg
     7981 cgttgatgga cactattgaa ctggcgggag cttgatcatg caagaatggt tccaaaacct
     8041 ctttgccgca acgctcggtc tgggcgattt gggtattacc gtaggcttgg tggtatccgt
     8101 catcgtcaaa attgtgatta tcctgattcc gctgattctg accgttgcct acctgacgta
     8161 tttcgaacgt aaagtcatcg gctttatgca gcttcgtgtc ggcccgaacg taaccggccc
     8221 gtggggtctg attcagccgt ttgccgacgt gttcaaactc ttgtttaaag aagtaacccg
     8281 tccgaagctg tcaaacaaag ccctgttcta catcggcccg attatgtcgc tcgccccgtc
     8341 ttttgcggca tgggcagtga ttccgttcaa cgaagaatgg gtgctgacca atatcaatat
     8401 cggccttttg tacatcctga tgattacctc gctgtcggtt tacggcgtga tcatcgcggg
     8461 ctgggcttcc aactccaaat attcgttcct gggcgcaatg cgtgcctccg cgcaaagcat
     8521 ttcctacgaa atcgccatga gtgccgcgct ggtgtgcgtc gtgatggtat cgggcagtat
     8581 gaacttctcc gacatcgttg ccgcacaagc caaaggtatt gccggcggtt cagtattctc
     8641 ttggaactgg ctgccgctct tcccaatctt catcgtctat ctgatttccg ccgttgccga
     8701 aaccaaccgc gcgccgtttg acgtggcaga gggtgagtct gaaatcgttg ccggacacca
     8761 cgtcgaatat tccggcttcg cattcgcact gttcttcctt gctgaataca ttttcatgat
     8821 tctgattgcc gcgctgacat cgctgatgtt cctcggcggc tggttgtccc cgttcccgca
     8881 aagttggggc tttatcggca caccttccgc attctggatg ttcgtgaaaa tggcggcggt
     8941 gttgtactgg tacctgtgga tccgtgcaac cttcccgcgc taccgttacg accaaatcat
     9001 gcgtttgggc tggaaagtgc tgattccgat cggcttcgcc tacatcgtgg ttttgggcgt
     9061 gtggatgatt tcaccgctga atttgtggaa ataagtttca gacggcatct tgaggccgtc
     9121 tgaacaaagc gattttgaat acctaacgaa atccctgttt tgagggaaca taatatggct
     9181 aacttagtaa aaacctttct gcttggcgaa ttggtaaaag gtatgggcgt aacgctcaaa
     9241 aactttttcg cccgcaaaga cacgatttat tttcccgaag agaaaacgcc gcaatccgtg
     9301 cgtttccgcg gcctgcacgc gcaacgccgt tatccgaacg gcgaagagcg ctgtattgcg
     9361 tgtaaattgt gtgaggcagt ttgtccggca atggcgatta acatcgaatc ggaagaacgt
     9421 gaagacggta cccgccgcac caagcgttac gacatcgatc tgaccaagtg catcttctgc
     9481 ggtttctgcg aagaggcctg cccgaccgat gcgattgtgg aaacgcatat ttttgaatac
     9541 cacggcgaga aaaaaggcga cttgcacatg accaagccga tcctcttggc aatcggcgac
     9601 aaatacgaag ctgaaatcgc caaacgcaaa gccgctgatg cgccgtatcg ttaaggagca
     9661 gacgaatgac tttttccgcg attctgttct atatccttgc cgccatcgtc ctatacggcg
     9721 cgctgcgtac cgttacagcg aaaaaccctg tccacgccgc tttgcatctg gtgttgacct
     9781 tctgcgtgag cgcgatgctt tggatgctga tgcaggctga gtttttgggc gtgacgctgg
     9841 tggtggttta cgtcggcgcc gtgatggtgt tgttcctgtt cgtcgtgatg atgttgaaca
     9901 tcgacattga agaaatgcgt gccggtttct ggcggcacgc gcctgttgcc ggtgtggtcg
     9961 gcacattgtt ggcagttgcc ctgattctga ttctggtcaa tccgaaaacc gaccttgccg
    10021 catttgggct gatgaaagac attcccgccg attacaacaa tatccgcgat ttgggcagcc
    10081 gtatttatac cgattatctg ctgccgtttg aattggcggc ggtattgctg ctgttgggta
    10141 tggtggccgc gattgcgctg gttcaccgta aaacggttaa tccgaaacgt atggatcctg
    10201 ccgaccaagt taaagtacgc gccgaccaag gccgtatgcg cttggtgaaa atggaagccg
    10261 tgaaaccgca aaccgaatct gccgaagaaa gcgaagtttc agacgacctc aagccgaaag
    10321 aggagggcaa agcatgatta ccttgacgca ttatctggta ttgggtgcgc tcctgttcgg
    10381 tatcagcgcg atgggtatct tcatgaaccg caaaaacgtg ctggtattgt tgatgtccat
    10441 tgagctgatg cttttggcgg tgaacttcaa ctttatcgcc ttttcgcaac atttgggcga
    10501 tactgccgga caaattttcg tatttttcgt actgaccgtt gctgctgccg aatctgccat
    10561 cggtttggca attatggtgc tggtgtaccg caaccgacaa acgattaacg ttgccgattt
    10621 ggacgagttg aaagggtaaa ggtaggttgg gtcgagacct gacaagacac cgatgccgtc
    10681 tgaaaacccg ataggaaaaa cgatgaaatc catagacgaa caaagcctgc ataatgcccg
    10741 ccgcctgttt gaaagcggcg acatcgaccg tatcgaagtc ggtaccaccg cgggcctgca
    10801 acagattcac cgttacctgt tcggcggctt atatgatttt gcgggtcaaa tcagggaaga
    10861 caacatttcc aaaggcggtt ttcgttttgc caacgccatg tatttaaaag aggctttggt
    10921 taaaatcgag cagatgcccg agcggacttt tgaagaaatc atcgccaaat atgttgaaat
    10981 gaacattgcc catccgtttt tggagggtaa tggcagaagt acccgcatct ggctggattt
    11041 ggtgctgaaa aaaaacctga aaaaagtcgt gaactggcaa aatgtaagta aaaccctgta
    11101 tttgcaggca atggaacgca gccccgtcaa cgatttagaa ctgcgctttc tgttaaagga
    11161 caacctgact gacgatgtgg acaaccgtga aatcatcttt aaaggtatcg agcagtcgta
    11221 ttattacgaa gggtatgaaa aaggctgagg gtcgtctgaa aagcgatttc agactgtttc
    11281 agacgacctg attcggtagg tgatcagacg ggagcggatg agaaaagaaa ttctgggtaa
    11341 gaataatccg gtctgaaata ttggaagaag aatgatggat aaaaatcagt tagaacaaga
    11401 atttcataaa gccatgttaa atatttatca ggaggctttg aatttgccgc aaccttacaa
    11461 ggcgacacga tttttacaaa ttgtaaatga atttggtggt aaagaggcgg cggataaatt
    11521 attaagtacg ggggaaaaga agactcagac cggttttaca gagctgattt tgagtggtgg
    11581 cggagtccac gccttgaaat acagtatgga atatctggtg ttacaaaagc cgtggtgtga
    11641 tttatttact gaagagcaat tagctgtggc acgcaaacga ttggagcgtg ttggatttgt
    11701 ttctccgaag taattttgta cgaaacaaac atagattttt aaatcaatcg gattcaatca
    11761 aatgaacgac atgactttat atctcatcat tgcccttgtg cctttggcag gctcactgat
    11821 cgcgggtttg ttcggcaaca aaatcggacg tgccggtgcg catacggtta cgatactcgg
    11881 cgtggcggtg tccgccgtgc tgtcggctta tgtgttgtgg ggctttattg acggcagccg
    11941 cgccaagttt gacgaaaacg tctatacctg gctgacaatg ggcggcttgg atttctccgt
    12001 cggctttttg gtcgatacga tgacggcgat gatgatggtc gtggtaacgg gcgtgtcgtt
    12061 gatggtgcat atctacacca tcggttatat gcacgacgaa aaagtcggct accaacgctt
    12121 cttcagctat atctctttgt ttacattcag tatgttgatg ctgattatga gcaacaactt
    12181 catccagctc ttcttcggct gggaggctgt gggcttagtg tcgtaccttt tgatcggttt
    12241 ctatttcaaa cgtccaagcg caacatttgc caacctgaaa gcctttttga tcaaccgcgt
    12301 cggcgacttt ggctttttgc tcggtatcgg cttggtgctt gcctatttcg gcggcagcct
    12361 gcgctatcag gacgtattcg cttatctgcc taatgttcag acggccacta tccaactgtt
    12421 ccccggtgtg gaatggtctt tgattaccgt aacctgtttg ctcctgtttg tcggtgcgat
    12481 gggtaaatcg gcacaattcc cactgcacgt ttggctgcct gattcgatgg aaggcccgac
    12541 cccgatttct gcattgattc acgccgcaac catggttacc gccggtttgt tcatggtatc
    12601 gcgtatgtcg ccgatttacg aaatgagcag cactgcgctg tcggtcatta tggtaatcgg
    12661 cgcgattacc gccctgttta tgggtttctt gggcgtgatt caaaacgaca tcaaacgtgt
    12721 ggttgcgtat tctaccctgt cgcaactggg ctacatgacc gtggctctgg gcgcgtctgc
    12781 ctattccgtg gcgatgttcc acgtcatgac tcacgctttc tttaaagcct tgttgttctt
    12841 ggccgcaggt agcgcgatta tcggtatgca ccatgaccaa gacatgcgcc atatgggcaa
    12901 cctgaaaaaa tatatgccgg ttacttggct gactatgctg atcggcaatt tgtcgctgat
    12961 cggtacgccg ttcttctccg gcttctattc taaagattcg attatcgaag cagcgaaata
    13021 cagcaccctg ccgggcagcg gctttgctta ttttgccgtc cttgccagcg tgttcgttac
    13081 cgcgttttac gcgttccgcc aatactttat ggtgttccac ggcgaagaga aatggcgcag
    13141 cctgcctgaa catcatgacg atcatcatgg tgaagaacat cacggcttgg gcaaaaacga
    13201 caatccgcac gaaagcccgc tggttgttac cctgccgctg attttgcttg ccatcccgtc
    13261 cgtcatcatc ggctatgttg ccatcgagcc tatgctttac ggcgatttct ttaaagacgt
    13321 gattttcgtc aataccgacg cgcatccgac catgcacatc atgaaggaag agttccacgg
    13381 cgcattggca atggtgtcgc acagcctgca ttcgcctgta ctctaccttg ctattgcagg
    13441 tgtcttaagc gcatggcttt tgtacgtcaa actgccgcac ctgcctgcga aaattgcaca
    13501 gacgttccgt ccgatttacg ttttgtttga aaacaaatac tacctcgacg ccctgtattt
    13561 caacgtcttc gccaaaggca cacgcgcatt gggtaccttc ttctggaaag tcggcgatac
    13621 cgccattatc gacaacggca tcgtcaacgg ctccgccaaa ctggtcggcg cgattgccgc
    13681 gcaagtgcgt aaagcccaaa ccggctttat ctacacttac gctgccgcta tggtattcgg
    13741 cgtattggta ctgctcggca tgaccttctg gggattgttc agataaggat gaggcttcag
    13801 acggtctctt aaaacgtcgt ctgacaaacc aacccatata acgatatttt tattttaacc
    13861 acaggttaac cattatgttt tccaactacc tactcagctt ggcaatatgg atacccatcg
    13921 ccgcaggtgt gctggttttg gcgaccggta aagacagccg tgcgccgctg gcgcgtgtgc
    13981 ttgccttcat gggtgcgctt gccggtttct tggtaacgct gcccttgttt accggtttcg
    14041 accgtttgag cggcggctat cagtttaccg agttccacga gtggattccg cttctgaaaa
    14101 tcaactacgc attgggtgtg gacggtattt cagtgctctt tattatcttg aatgcgttta
    14161 ttacgctgtt ggtggtattg gccggttggg aagttattca gaaacgtccg gcgcagtata
    14221 tggcggcatt cctgatgatg tcgggtttga ttaacggcgc gtttgccgca caggatgcga
    14281 ttctgtttta tgtgttcttc gagggtatgc tgattccgtt gtacctgatt atcggtgtat
    14341 ggggcggccc gcgccgtgtc tatgcgtcgg tcaagctgtt tctctatacg ctgatgggtt
    14401 cgctcctgat gctggttgcc atggtttacc tgtactatca aacaggcagc ttctctattg
    14461 tcgatttcca aaacatcaaa cagattccgt tgggcgtaca gcagcttttg tttgcggcat
    14521 tcttcctgtc atttgccgta aaagtgccga tgttccccgt acacacttgg ctgccggatg
    14581 cccacgttga agcgccgacc ggcggttcga tggtgttggc ggcgattacg ctgaaactgg
    14641 gtgcgtatgg tttcttgcgc tttatcttgc cgattatgcc ggacgcggca cgctattttg
    14701 cccctgtgat catcgtattg agtctgattg ccgtgattta catcggtatg gtggctttgg
    14761 tgcaaaccga tatgaaaaaa ctggtggcgt attcgtccat cagccatatg ggttttgtaa
    14821 cgcttgggat gtttttgttt gttgacgggc agttggacga ctgggcgttg aaaggcgcgg
    14881 tcattcaaat gatttcccac ggtttcgtgt ctgccgcgat gtttatgtgt atcggcgtga
    14941 tgtacgaccg cctgcacacg cgcaatattg ccgattatgg cggcgtggtc aatgtgatgc
    15001 ccaagtttgc ggcgtttatg atgttgttcg gtatggcgaa cgccggtttg cctgcaactt
    15061 ccggcttcgt gggcgagttt atggtgatta tgggcgcggt taaagtgaat ttctgggtag
    15121 gcgctttggc cgccatgacc ctgatttacg gtgcgtctta taccctgtgg atgtacaagc
    15181 gcgtgatttt cggcgcgatt cacaatccgc acgttgccga aatgaaagac atcaactgcc
    15241 gcgaatttgc gattctggcg gtgttggcgg ttgctgtttt gggtatgggc ctgtatccga
    15301 acgcatttat cgaagtggtg catcaggcgg caaacgattt gattgcccat gtggcacaaa
    15361 gcaagatgtg aggtgtgtaa atgaactggt ctgatttgaa tttaatgccc gccatgcccg
    15421 aaatcgtgct gcttgcgctg ctggtgttat tgttgctggc ggacttgtgg gtcagtgatg
    15481 acaaacgccg ttggacgcat tacggcgcgt tggcaacggt ggcggttacg gctgtggtgc
    15541 agcttgccgt ttgggaacag ggcagcacgt cttcgttcaa cggcatgtat attgcagacg
    15601 gtatgtcgcg tttggcaaaa atggttttat atgccttgac gtttgccctg tttgtttatg
    15661 ccaagcccta caaccaagtg cgcggtattt tcaaaggcga gttttacact ctgtcgctgt
    15721 ttgccctctt gggcatgagc gtgatggtga gcgcggggca ttttttaact gcctatatcg
    15781 gtttggaact cttgtcgctc gccctttacg ccctgattgc cctgcgccgc gattccggct
    15841 ttgccgccga agccgccttg aaatattttg ttctgggcgc gctggcatcc ggcctgctgc
    15901 tctacggtat ttctatggtt tacggcgcaa ccggttcgct ggaatttgcc ggcgtgctcg
    15961 cctcttcctt caatgaagaa gcaaacgaat ggctgttgaa actgggtctg gtgtttatcg
    16021 tcgtcgccgt tgcgttcaaa ctcggcgcgg tgccgttcca tatgtgggtg cccgacgtgt
    16081 atcacggcgc gcccacttct gttaccgccc tagtcggcac tgccccgaaa atcgccgccg
    16141 tcgttttcgc tttccgcatc ctcgttaccg ggttgggaac cgtgcatcat gactggtctc
    16201 tgatgtttgc cctgcttgcc gccgcctcgc tgctggtcgg caaccttgcc gccatcatgc
    16261 agaccaatat caaacgtatg ctcgcctatt ccaccgtatc gcatatgggg tttatcctgc
    16321 tggcgtttat ggcgggcgcg gtcggctttg cggcggggct gtattacgcc attacctacg
    16381 cgctgatggc ggcggcaggg ttcggcgtgt tgatggtgtt gtcggacggg gacaacgagt
    16441 gcgaaaacat cagcgatttg gcagggttga accaacaccg cgtatggctt gcctttttga
    16501 tgctgctggt tatgttctcc atggcaggca ttccgccgct gatgggtttt tacgccaaat
    16561 tcggcgtgat tatggcactc ttgaaacaag gctatgtttg gttgtccgta tttgccgtca
    16621 tcatgtccct gattggcgcg ttctactacc tgcgcgtggt caaagtcatg tattttgacg
    16681 aatccggccg cgcccatccc accgtcggcg gcaacaatgc cgcaaaattc cttttgagcg
    16741 tcaacgcgct gctgttggtg ctgtggggca ttatgccgca aaccgttatc gactggtgcg
    16801 ccaaggcgtt ggaaaacacg ctgtaagccg ccgcaacggc agccgtgtca gaggctgccg
    16861 tttttgttaa gatatgccgt tccgcaacgc agttcagacg gcatcgccgc cgacaatgcc
    16921 caaacagaaa gcccgccatg accgcatcca tgtacatcct tttgctgctt gccttgattt
    16981 ttgccaacgc ccccttcctc acgaccaggc tgttcggcat cgtaccgctc aagcgcaaac
    17041 atttcgggca ccacttgatc gagctggcgg caggtttcgc gctgaccgcc gttcttgcct
    17101 acatcctcga atcccgtgcg ggagcggtac acgatcaggg ttgggagttt tacgccaccg
    17161 tcgtctgcct gtacctgatt tttgcgtttc cctgtttcgt gtggcggtat ttttggcaca
    17221 cgcgcaacag ggaataggca ggcataggaa tgccgtctga aaccctttca gacggcattt
    17281 gtttcattca agtgcaggcc ggcatcgctg tgccggcacg tttcagccgg cgatatacgc
    17341 cggttttaat atttgcgggc gactgcaaat tctgccaact gccgcaggcg cagggctttg
    17401 tcgccgaagg gttcgagcag cgcgaccgct tcggcaacca gtttgtgtgc gtatgagcgc
    17461 gccgcttcca agcccatcag tttcacataa gtcggcttgt cgttgtctgc gtctttgccc
    17521 gccgttttgc ccaaagtcgc cgtgtccgct tcacaatcca acacatcgtc aatgacttgg
    17581 aacgccagcc ccagttttgc cgcataatcg tccaataccg ccagactgtc ttcagacaga
    17641 ttcgggcagg acatcgcccc caataaaacc gccgcacgga ttagcgcacc cgttttcagg
    17701 ctgtgcatct gttccaaatc ggcttgaacc atttgtttgc cgacattcgc caaatcgatt
    17761 gcctgaccgc ccgccatacc cctgcttccg cccgcttttg ccaaaactga caacattgcc
    17821 agctgccgcg aagcaggcag gccggacgga cgcgccaata catcgaaggc ttgggtttgc
    17881 aaagcgtcgc cggtcagcag tgcgtttgct tcgccatatt tgatgtggca ggtcggtttg
    17941 ccccggcgca ggctgtcgtt gtccatcgcc ggcatatcgt cgtgaaccag cgaataggcg
    18001 tgaatcatct cgattgccgc cattgcctgt cctactgctt catgcacggc ttcgcctaat
    18061 tccgaagctg ccagaaccag catcggccgc agacgcttgc cgccgtccaa agccgcataa
    18121 cgcatcgctt cgtgcagcgt gtgcgggatt tcgttttcag acggcaaaaa gcgttccagc
    18181 agcagctctg tttgcgcctg cgccctctgt tgccacgctt tcaaatcatt cgtcggattc
    18241 aaggttcaac tccttcggcc cgtccgcgtc caaaacctgt aatttctgtt cgacttgcgc
    18301 cagcttggtt tggcagtacc tgaccagctc gttgccttcc tgataggcgg caagcgcgtc
    18361 ttccaagggc atttcaccct gcatagcttg tgtcagcgat tcaaggcgcg acagtgcctc
    18421 ttcaaacgat ttcggggcgt ttttcttcat agtttcgggt gttccggtaa aaagcggatt
    18481 gtagcatttt tgccaaacgg atgccgtctg aaacccgaat ccggcttcag acggcattgt
    18541 tttgtcctgc aaacggcaag gcgttacccg ggcagttcgt cggtgatgcc ctgcaaaaag
    18601 gcgaggcgtt cggggcttgc cgccccggtt tgcgcggcgg ctttgaaggc gcagccgggt
    18661 tcggcgcggt gggtgcagtt gtggaagcgg cattgcccga caaggtggcg gaaatcgggg
    18721 aaatagcgcg gcaaatcggc ggcttggagg tggtgtaaac caaattcttg caaacccggg
    18781 gagtcgatga gttgggtttc gccgttcaaa tcataaagcc gggcgtgggt ggtggtgtgt
    18841 tttcccgagt cgagtgcggc ggaaatgtcg ccggtgcggg cggtttggct gcccaaaagg
    18901 gcgttggtca gggtggattt gcccataccg ctctgcccga gcaggatgtt gctgtgccct
    18961 tgcagggcgg ggcgcaggct gccggcgttt tccagtgcgc gggtttcgat gacgggataa
    19021 cccagcgttt cgtagaattt gagtttttcg cgccaaaggg cggtttcggg caggtcggct
    19081 ttgttcagga cgatgacggc ttcaataccg gcggcttcgg cggcaagcag ggcgcgttgc
    19141 agcagccgca cgctcggact cgggacggcg gcggttacga tgaggagttg ggtaacgttg
    19201 gcggcgatga gtttggtttt ccacgcgtct tggcggtaga gcaggctttg gcgcggtaaa
    19261 aaatcttcaa tcacaacttg ttcggcgttg acggggctga tgcggacacg gtcgccgcag
    19321 gcgaaatcga cgcgtttttt gcgggtgctg gcttcgtagg ttgtgccgtc gggcgtgcgc
    19381 acgatgtagc ggcggccgta gctggcggta atttgggcgg tgtcgttcat ggtttctttg
    19441 gggttgggtg tggaaatgcc gtctgaaaac gggtgttcgg acggcatcgg ttcagtcgtg
    19501 ctgccactcg acgtgttcgt tgaggaagcc gccgctctgg tgcgcccaga gtttggcgta
    19561 aagcccgcgt ttttcgagga gttcggcgtg tgtgccttct tcgatgatgc ggcctttgtc
    19621 gaggacgacg agcctgtcca ttgcggcgat ggtggagagg cggtgggcga tggcgatgac
    19681 ggttttgccg tccatcattt tgtcgaggct ttcctggatg gcggcttcga cttcggaatc
    19741 gagcgcgctg gtggcttcgt cgagcagcag gatgggcgcg tctttgagca tcacgcgggc
    19801 aatggcgatg cgctggcgtt gcccgccgga gagtttcacg ccgcgttcgc cgacgtgcgc
    19861 gtcatagccg cgccgccctt tggcatcgga aagttcgggg atgaagccgg cggcttcggc
    19921 gcgttcggcg gcggaaacca tttcggcatc ggtcgcgtcg gggcggccgt aaataatgtt
    19981 gtcgcgcacg gaacggtgca gcagcgaggt atcttgcgtg accaaaccga tttgggcgcg
    20041 taaagattct tgggtaacgc cgcttatgtc ctgcccgtcg atcgaaaccg tgccgctttg
    20101 cggttcgtag aagcgcaaaa gcaggttgac gatggtggat ttgcccgcgc cgctgcgtcc
    20161 gatcaagccg actttttcgc ccgggcggat ggtgaggttg aagccgttga gcagcggttt
    20221 gccggcttcg tagcagaaat cgatgtgttc aaatttgatt tcgccttgcg acactttcag
    20281 cggcagggcg ttcggcttgt cgaggatggt gtgcggtttg gacagggttg ccatgccgtc
    20341 gccgacggtg ccgatgtttt caaacagccg cgcggattcc cacataatgt attgcgacag
    20401 cccgttgacg cgcaacgcca tcgcggtggc ggtggcgacc gcgcccacgc cgacctgccc
    20461 gttgtgccag agccagatgc ccagtgcggc ggtggagagg gtcagggagg tgttgacgat
    20521 gaagctgcac gaatgcagca gcgtcgccag ccgcatttgg gcgcgcaccg taaccataaa
    20581 ttcttccatc gactgcttgg cataggcggc ttcgcgcgcg ccgtgggaga agagtttgac
    20641 ggtggcgata ttggaatagg catcggtaat gcggccggtc atcagcgagc gggcatccgc
    20701 ctgccatgcg gcggtttgcc ccaatttggg aatcagcagg cgcatcaccg aagcgaaacc
    20761 gacaatccag ccgataaagg gcagcagcag ccatgagtcg agcgaggcga gaatcacgcc
    20821 ggaggtaatg aaatacaccg acacataaac gaccatatcg gcaaccgtca tcaccgcgtc
    20881 gcgcaacgcc agcgcggtct gcatgacttt ggcggacacg cgtccggcaa attcgtcctg
    20941 ataaaaaccg aggctttggt tcagcatcag gcggtggaag ttccagcgca ggcgcatagg
    21001 gaacacgccc tgaagggttt gcaggcgcac gttggacgcg gcatacgccc acacgaccga
    21061 aaacaccatc atcgccgcca ttgccgccag ttcccaacct ttttcggcaa acagttcggc
    21121 gggcgcgtat ttgccgagcc actccacgat tttgcccata aattgaaaaa ccagggcttc
    21181 cataatgccg atgccggcgg tcagcgcagc cagggcggct atccatttcc gcacgccggc
    21241 catgctgctc cagacaaacc gccacaagcc tttttctggc gttttcgggg cggcttcggg
    21301 ataagggtcg attcgggact cgaaccagga aaatattttg ttcaacattg ttttcgattt
    21361 cggtaaaaca gtttcagacg gcatcaaaca caatgccgtc tgaaaggaag gacaataacg
    21421 ccattttacg ggaaaagccg tcgggaagac agcgcgaggc ggaaacgcag ggtttcgtca
    21481 gggcaaacgc cgcgccgcct tcagacggca ttatttcagc aggtttttca aagcaaggcg
    21541 cacgccttcg cccacgtccg tccctttcgg aacgcctttg accgccgctt ttgcttcgcg
    21601 ttcgctgtaa cccagtgcaa gcagtgtgct gacgatgtct tccgtttcgg cggcggcggg
    21661 tgcggcggca aacagcccgt ccgttaccgt atgcgcgacc agcttgccgc gcagttccaa
    21721 gaccatacgt tcggcggttt ttttgccgat tcccggcgcg gaagacaggc gtttgacatc
    21781 ttcttctgca accgcccgcg ccagctcgtc tgccgtcatc gctgacaaaa tgcccaaagc
    21841 cgttttcgcg ccgatgccgc cgaccttaat cagttggcgg aaggtcttgc gttcttccgc
    21901 agtggcaaaa ccaaataaaa gatgtgcgtc ttcccgaatg ataagctggg taaacagttg
    21961 tacgctttca cccacgggcg gcaggttgta gaaggtctgc atcgatacgt cggcctcata
    22021 gccgacaccg ttgacatcga tgacgatttg cggagggttt ttttcaacca gtttgccggt
    22081 cagtctgctg atcatgtatg aaggatcctg aagtgtcggg tgcaaaatgc cgtctgaaac
    22141 cggttcgggc ttcagacggc acggattgta tcaaattcag tcgtcgcggc gggaggaaat
    22201 cacgcggccg gtacgggcat cgacaacgac tttgtattcc tgtccgtttt tgacgatttc
    22261 gacatcatag tgcggacggc cgttgtcgtg ttcgagatcg atgtcggtga ttttgccgcc
    22321 gacacgcgcc aacgctgctt tttcggcttg ggcgcggctg atgattttgt cttgtttgct
    22381 gtgttggtgt gcggcgtgtc cgtggtcgtc atcgccgtgt ccgtcgtggt gggcgagtgc
    22441 gggggcggaa atgcccatca gtgcggttgc ggcggcggtc aagagaaggt gtttgatgtt
    22501 catattttgc ctttgtaaat cgtgggttgg aaaatgtgga tattaataag gtatcaaata
    22561 accgtcagcc ggcagtcaat accgcccgaa ccataccgcg cgcctgagct tcggcttcgg
    22621 cggcgcgttc ctgcgaggaa aacggcccca ttttgacgac atattcgtaa tggcgttttt
    22681 caaccgatag gttcgggttt gatgccgatg aagccaggtt ttgggcggct tggttcagat
    22741 aggcttgtgc ttcgtgttcc gtaccgaaag atttcaagtc gatgaagatg tctttgtttt
    22801 cggcaaccag tgcggattgg cccgggacga tttgttcgat tttgacgtgc gccgtccctt
    22861 ggctgacaaa gcccaatttt tgcgcggcgg ctttggatac gtcgatgatg cggttgccgt
    22921 ggaagggacc gcggtcgttg acgcggacga tgacgctttt gccgtttttg gtattggtta
    22981 cgcgcacata gctggggatg ggcagggttt tgtgggcggc ggtaaaggcg ttcatatcgt
    23041 atcgttctcc gccggaagtt ttgcgcccgt gaaacctgcc gccgtaccac gaggcgttgc
    23101 cggtttgcgt gaattcggcg acttggtttt tcggcgtgta gcgttttccg gcgactttgt
    23161 agctgcggtt ggcggaggcg tgcagttttt ctgccttgac cactgcgtcg gcggatgccg
    23221 tctgaaggga gtgtgtgccg aatgcggcgg tgagaaggaa aagggttttt cgggttaaag
    23281 tcaaaacgtg ttccgttctt gagttgaaga cgaatgggca tcatgcccgc cggacgcgtt
    23341 ccgaaccgcc gagcgctgcg gcagcggttc ggaatgcgtc cgaacggttt tcagacggca
    23401 tgaacctgcg ttcaaacgcc gcctgcgtaa ccgtgttgcc gccacgcttc aaagagaatc
    23461 acggcaacgg tattggaaag gttcatactc cggctgccgg gctgcatcgg caggcggatt
    23521 ttttgcgcgg cgggcaggct gtcgaggatg tcggcaggca gtccgcgcgt ttccggcccg
    23581 aacagtaaaa cgtcgccttt ttgaaacgcg gtttcatcgg ggcgcgccgt gcctttggtg
    23641 gtcagggcga aaatgcgcct gcctgcgagt gccttgaggc agtcgtcgaa gttttcgtac
    23701 accgtcaggc tggcgaattc gtggtagtcg agcccggcgc gtttcatttt ggcggaatcc
    23761 aatgggaagc cgagcggttt gacaaggtgc aaatccgcgc cggtattggc gcacaggcgg
    23821 atgatgttgc ccgtgttcgg cgggatttcc ggctggtata aaacgatggt aaacataaat
    23881 atcaatcact tataggcgcg taaccttgcc acaaggcgga tggggtgtca aaaaatttag
    23941 ttattttttc attggcgtgc gtgccagcgt ccagcagcag attcggtttg cgcccgattt
    24001 tttcagcatc ttcgccaatt cgtccagcgt cgcgccggtg gtaaagacat cgtcgattaa
    24061 caaaatatta cagcctttcg gcagcggttc gcggatttca aaggcgtttt tgatgttttt
    24121 caggcgttcg ccgcctttga gcgtgctttg cggcggccgg tggcggcgga atacggtatg
    24181 caggggcagc gtgcgccaac cgtagcgttg agccagcagc ccgacgatgc tttcactttg
    24241 gttgaacccg cgttgcagca gccgctccct gcttagcggt acgggcagga cgaaatcgaa
    24301 acattcgtct gcaagccggt cgggcggatt ctgcatcatc aggtctgcca gcggctgcac
    24361 catgctcaaa tcagccaagt gcttcagcgc gtgtatcata ttgctgacgg gcggttcgta
    24421 atgcagcgaa gcccacatcc ggtcgaatgc gggcggtttt ttctgacagc cgccgcacac
    24481 cgcgccgcct tgaacgtgca tgaaacacag ggggcagctg tttgccgcgt cggtgcggta
    24541 tgccgccaaa tcgtcgcggc agccggcgca gatgccgtct gaaacgccgg acgaaccgtg
    24601 gcataatacg caacgcctga tagtgggcgc gtctgcgatg cgccgccaac aagagagaaa
    24661 atccatgcct gatgccgtca aaaaagttta cctgatacac ggttgggggg cgaaccgcca
    24721 cgtgtttgac gatttgatgc cgcgcctgcc tgcaacgtgg gcggtgtccg ccgtcgattt
    24781 gcccggacac ggcgacgcgc ccttcgtgca acccttcgat attgaggcag ccgccgacgc
    24841 tgttgccgcg caaatcgata cgcccgccga cattctcggc tggtcgctcg gcggattggt
    24901 cgcgctgtat ctggcggcgc gccatcccga caaagtccgt tcgctctgcc tgacagcgag
    24961 tttcgcacgg ctgacggctg ccgaagatta tcccgaaggg cttgccgcgc ctgcattggg
    25021 caaaatggtc ggcgcgttcc gttcggatta tgccaaacat atcaaacagt ttctacaact
    25081 ccagcttctg cacacgcctg atgcggacgg aatcataggc agaatcctgc ccgatttggc
    25141 gcgctgcggc acgccctccg ccttacagga agctttggac gcggcggaaa gggcggatgc
    25201 gcggcatttg ttggacaaga tagatgttcc ggtactgctg gtgttcggcg gcaaagacgc
    25261 gattacgccg ccgcgtatgg gtgaatatct gcaccgccgt ttgaagggca gcaggttggt
    25321 tgtgatggaa aaggcggcgc atgcgccgtt tttgagccat gcggaagcgt ttgccgcgct
    25381 gtaccgcgac tttgtcgaag gggtttgaga tgaaccatca ggacgcacgc tggcaggttc
    25441 accgccatct tgccgaacat accgaccaac gcctgacact cgtccgcaac gcgcccaagt
    25501 atatcctgct tgcaggtgcg gatgcggaca tcagccgcag cctgctggcg aaacgctatc
    25561 cgcaggcggt atttgaagaa tacgattccc gtgcggattt tttggcggca gccgccgccc
    25621 gcaaaggcgg tttttggcaa aggtttgcgg gtaagggcgt ggtgcagcac tgccaatccc
    25681 cgaccgcgcc gctgcccgaa gcgtgtgccg atatgttgtg gtcgaatctc ggactgttgg
    25741 cggcggaaca aatccttcct gtgctgcaca actgggcgcg cgccttgaag acggacgggc
    25801 tgctgttttt tacctgcttc gggcgcgaca cttttaccga actgaaatgc cgtctgaaag
    25861 aaaacggcat tgaaagccgc agcgtgatgt ttcccgatat gcacgacttg ggcgatatgc
    25921 ttgctgaaaa cggcttttac gaccccgtta ccgatacggc gaagctggtg ttggattaca
    25981 aaaaggcgga aacgttttgg gcggatatgg acacgctggg cgtttggcgg gcgatggcgt
    26041 ggaacgatga aaacgccgcg cgttcgtgtg tcgggacaat atttgaacgg gaaggcggtt
    26101 tgagtattac gctggaaaca gtgtacggac acgccgttaa aaaactgatg ctgccgcaag
    26161 gggagaacgt ggtgcagttt tttccgaaga gatgatgtgc agatgccgtc tgaagccgtt
    26221 tccaagtttc agacggcatt tgtttgtgaa aaccgacaga actaaaggaa atgccgatgt
    26281 atagtgaatt aaatt
//
LOCUS       DAXHRI010000011        26124 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXHRI010000011 DAXHRI010000000
VERSION     DAXHRI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.011"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(55..1239)
                     /gene="odhB"
                     /locus_tag="ACFM75_000335"
     CDS             complement(55..1239)
                     /gene="odhB"
                     /locus_tag="ACFM75_000335"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC6254935.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEATPAAAAPD
                     AAQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAASAPAVALPA
                     GARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHG
                     VKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDA
                     DQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAI
                     LGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLL
                     DL"
     gene            complement(1339..4167)
                     /locus_tag="ACFM75_000336"
     CDS             complement(1339..4167)
                     /locus_tag="ACFM75_000336"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC6254936.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVVGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     STRAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4372..5655)
                     /gene="gltA"
                     /locus_tag="ACFM75_000337"
     CDS             complement(4372..5655)
                     /gene="gltA"
                     /locus_tag="ACFM75_000337"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC6254937.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDIANPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYAENFLHMMFATPCEEYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5767..6015)
                     /locus_tag="ACFM75_000338"
     CDS             complement(5767..6015)
                     /locus_tag="ACFM75_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC6254938.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6019..6726)
                     /locus_tag="ACFM75_000339"
     CDS             complement(6019..6726)
                     /locus_tag="ACFM75_000339"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC6254939.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6846..8609)
                     /gene="sdhA"
                     /locus_tag="ACFM75_000340"
     CDS             complement(6846..8609)
                     /gene="sdhA"
                     /locus_tag="ACFM75_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC6254940.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8612..8953)
                     /gene="sdhD"
                     /locus_tag="ACFM75_000341"
     CDS             complement(8612..8953)
                     /gene="sdhD"
                     /locus_tag="ACFM75_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC6254941.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8947..9324)
                     /gene="sdhC"
                     /locus_tag="ACFM75_000342"
     CDS             complement(8947..9324)
                     /gene="sdhC"
                     /locus_tag="ACFM75_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC6254942.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9603..11006)
                     /locus_tag="ACFM75_000343"
     CDS             complement(9603..11006)
                     /locus_tag="ACFM75_000343"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6254943.1"
                     /translation="MKKIQADVVVLGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDSFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAS
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11255..11992)
                     /locus_tag="ACFM75_000344"
     CDS             complement(11255..11992)
                     /locus_tag="ACFM75_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC6254944.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12271..14547)
                     /gene="metE"
                     /locus_tag="ACFM75_000345"
     CDS             complement(12271..14547)
                     /gene="metE"
                     /locus_tag="ACFM75_000345"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC6254945.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTELAAAGAEWIQIDEPALAVDLPQAWL
                     DAYQDVYATLSQVNAKILLSTYFGSVAAHAGRLKALPVAGLHIDLVRAPEQLDAFADY
                     GKVLSAGVIDGRNIWRANLNNVLATLEPLKAKLGDRLWISSSCSLLHTPYDLSVEEKL
                     KVNKPALFSWLAFTLQKTQELRVLKTALNQGREAVAAELADSQAAADSRANSTEIHRA
                     EVAKRLADLPADADQRKSPFAERIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSDADYEAAMKKEIALVIEEQEKLNLDVLVHGEAERNDMVEYFGELLEGFAFTQY
                     GWVQSYGSRCVKPPVIYGDVSRPNAMTVAWSTYAQTLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(14685..15563)
                     /gene="metF"
                     /locus_tag="ACFM75_000346"
     CDS             complement(14685..15563)
                     /gene="metF"
                     /locus_tag="ACFM75_000346"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC6254946.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15763..16038
                     /locus_tag="ACFM75_000347"
     CDS             15763..16038
                     /locus_tag="ACFM75_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC6254947.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16038..16163
                     /gene="ykgO"
                     /locus_tag="ACFM75_000348"
     CDS             16038..16163
                     /gene="ykgO"
                     /locus_tag="ACFM75_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC6254948.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16269..16469)
                     /locus_tag="ACFM75_000349"
     CDS             complement(16269..16469)
                     /locus_tag="ACFM75_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254949.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRKQKRALRDVAKISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            16627..17766
                     /locus_tag="ACFM75_000350"
     CDS             16627..17766
                     /locus_tag="ACFM75_000350"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC6254950.1"
                     /translation="MSQNASVGIVTPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVIGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17763..18344
                     /gene="metW"
                     /locus_tag="ACFM75_000351"
     CDS             17763..18344
                     /gene="metW"
                     /locus_tag="ACFM75_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC6254951.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRILERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18461..19306
                     /locus_tag="ACFM75_000352"
     CDS             18461..19306
                     /locus_tag="ACFM75_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC6254952.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19356..19916)
                     /gene="efp"
                     /locus_tag="ACFM75_000353"
     CDS             complement(19356..19916)
                     /gene="efp"
                     /locus_tag="ACFM75_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC6254953.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(19959..21107)
                     /gene="earP"
                     /locus_tag="ACFM75_000354"
     CDS             complement(19959..21107)
                     /gene="earP"
                     /locus_tag="ACFM75_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC6254954.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRDA
                     VRFDTEALRERLMLPEKNASEWLLFGYRSDVWAKWLEMWQQAGSPLTLLLAGAQIIDS
                     LKQSGIIPQNALQNDGDVFQTASVCLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRRLSDDLNGGGALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21126..21404)
                     /locus_tag="ACFM75_000355"
     CDS             complement(21126..21404)
                     /locus_tag="ACFM75_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254955.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
     gene            21584..22525
                     /gene="miaA"
                     /locus_tag="ACFM75_000356"
     CDS             21584..22525
                     /gene="miaA"
                     /locus_tag="ACFM75_000356"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC6254956.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            22529..23248
                     /gene="tadA"
                     /locus_tag="ACFM75_000357"
     CDS             22529..23248
                     /gene="tadA"
                     /locus_tag="ACFM75_000357"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC6254957.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKARLLAELKNHPPVAAFPPQEEMEHFMCEALRQ
                     AEQSSADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGREIQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            23253..23741
                     /locus_tag="ACFM75_000358"
     CDS             23253..23741
                     /locus_tag="ACFM75_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC6254958.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            23807..24559
                     /gene="rlmB"
                     /locus_tag="ACFM75_000359"
     CDS             23807..24559
                     /gene="rlmB"
                     /locus_tag="ACFM75_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC6254959.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVAGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(24621..26012)
                     /locus_tag="ACFM75_000360"
     CDS             complement(24621..26012)
                     /locus_tag="ACFM75_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFC6254960.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLIFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
BASE COUNT         6502 a         6950 c         6492 g         6180 t
ORIGIN      
        1 ctataaaaat tcagacggcc ttttcattaa caaataaaag gccgtctgaa acgattacag
       61 atccaacaac aggcgggctg ggtcttccaa cgcgtcttta atggctacca aggtcaatac
      121 agcttcgcgg ccgtcaatga tacggtggtc gtaagacaga gccagataca tcattggacg
      181 gacaacaact tggccgtttt caaccacagc gcgctcttta gtggcgtgca tacccaaaat
      241 cgcagattga ggcgggttga tgatcggggt agacatcata gaaccgaaag taccgccgtt
      301 ggtaatactg aatgtaccgc cggtcagatc ttcgatagcg attttgccgt ctttggcttt
      361 tttcgcgtaa tcaacaattg cttgctcgat gtcggcaatg ctcatttggt cggcatcacg
      421 caggattggt acaaccaaac cgcgtgggct gccaattgcg ataccgatgt cgaagtagcc
      481 gtggtacacg atgtctttgc cgtcaacaga agcattcaca accgggtatt ttttcagggc
      541 ggcaacagcg gctttaacga agaaagacat aaagcccaat ttcacgccgt gttctttctc
      601 gaatttttct ttgtacttcg cacgcaagtc catgattggt ttcatgttga cttcgttgaa
      661 tgtagtcaga atggcgtttt cttgttgaga agccaagagg cgttctgcaa cacgggcacg
      721 caggcggctc attggtacgc gttcttcagg acgtgcgccg gcaggaagtg caacggcagg
      781 agcagaggct gcggcaggtt tggctgcggc attttgtacg tcttctttca atacgcgacc
      841 gtcacggccg gaaccttgca atgcgttcac gtcaacaccg gtctcggcag ccagtttggc
      901 ggcagcaggc atagcggcgt tgttttgtgc agcatcagga gcggcggcgg ctggggtagc
      961 ttcggcaggc gcggcggcag gggcttcagc agcggcagta gcagcggtat cgatacgtgc
     1021 caaaacttgg tcggcaacaa cggtttcacc gtcttgcgct acgatttcaa ccaatacgcc
     1081 ggcttgtgga gaaggtactt ccaaaaccac tttgtccgtt tcgatatcaa tcaggatttc
     1141 gtcacgggca actgcttcgc caactttttt cttccattcc aagagcgtgc cttcagatac
     1201 gctttcagac agcataggta cttttacatc aataatcatt ttgtgtctcc aatggccctt
     1261 tcagacggcc tgttgtgtgt ttttcattct gcatatgggc attccgcctg agcagcctca
     1321 gacggaatgc tgcttggttt acaacgccaa agcgtcttca accaattgtt tcaattgagc
     1381 aatgtgtttg cttgagtagc ccactgcagg cgatgcgctg cttggacgac cagcataaga
     1441 cagtttttgc tcttcgctga taacgtcttc gatgcggtgg cggatttggt agaacgcgcc
     1501 ttggtttttc ggctcttctt gcgcccaaac cacagatttt gcgttcggat atttcgccag
     1561 ttcggcttta acctcatcgt atgggaacgg atacagctgc tcaacgcgga caatcgcaac
     1621 atcatcttcc agtttacgtt cggctcgacc ggcttccaag tcatagtaaa cctgaccggc
     1681 acacaatacc acgcgtttca cgctgtcgtt gcttgcgcgt tcggcggtat cgccgataac
     1741 cggacggaaa gtcgaacctt cggtgaagtt ttccagcggg ctcattgccc ctttgaatcg
     1801 caacaggcgt ttggacatga aaatcaccag cggtttgcgg tatgaaccta agacttgacg
     1861 ttgcaagagg tggaacattt gcgacgcttc agatggcatg atgacttgca tattgttctc
     1921 agaacacagt tgcaaccagc gttctacgcg tgcagaagag tgctcagggc cttgaccgtc
     1981 gtaaccgtgc ggcaggatgg tagtcagacc gcacaagcga ccccatttgg tttcgcctga
     2041 agacaggaat tggtcaatag tcacttgcgc gccgttggcg aagtcaccga attgagcttc
     2101 ccaaatggtg agcttgtcag gagcggagca ggcaaagccg tactcgaacg ccatcacggc
     2161 ctcttcgttc aagatggagt cgataaccag gaactcgccc attccttcgc ccatatggcg
     2221 caggggaaca taagtaccgt cgtcccattt ttcgcgtttt tgatcgtgca atacggcgtg
     2281 gcggtgcgag aacgtaccgc gtcccgagtc ctcaccggaa atacgcacgc cgtgaccttt
     2341 ggttacgagg ctggcgtatg ccaaggtttc ggccataccc caatctatgg cctgtttgcc
     2401 ggatgccatg gctttacgcg cttcaatcac acgttttgca gtcggatgca gggcaaagcc
     2461 ttccggtacg gcggtaaact tctcagtgag acgttcaata tctgcggcag gcaaaccggt
     2521 ttcgatgtgt tcgcgccaat ctttgccttg gtatttgctc cagtcgattt gcgtgcgttg
     2581 gaagttgctc aacgttgttt gctcgacgtg ctcgcctttg tccaaagcat cacggtaggc
     2641 ttggatgtaa ccgtctgcct cggcttgggt taccacgcct tcggcaatca gttgctcggt
     2701 gtacaaagca cgcgcacccg ggtgttgcga tacttttttg tacatcatcg gttgggtcaa
     2761 ggtcggatca tcgccctcgt tgtggcccca tttacggtag cagacaacgt cgatcacgat
     2821 gtctttatgg aattttttgc ggtaatccaa agcggcttgg atggcaaagc aaacgcgctc
     2881 aggatcatcg ccgttcacgt ggataaccgg agcagaaacc attttcgcga tatcggtaca
     2941 gtgtacggtt gaacgggtat cgcggatatc ggaagtggta aagccgattt ggttgttgat
     3001 cacgatgtga accgtaccgc cggtggtata accacgggtt ttagacaggt tgaatgttgc
     3061 ttggttaaca cccaaaccga taaatgcgga gtcaccgtga atcaataccg gcaagacttt
     3121 atcacggccg ttttcgccca aacgtttttg tttggcgcgt gtagaacctt ccactaccgg
     3181 gttaacaatt tccaagtgtg acgggttaaa tgccaaagaa acgtgcatcg gaccatgtgg
     3241 agtggcgatg tcggagctga agcccatgtg gtatttcacg tcgccgctag gcaatttgat
     3301 ttcggcacgg ccttcaaact cagcgaacaa atctccgggt tttttaccca aaatgttaac
     3361 caaaacattc agacgaccac ggtgcgccat accgatgatg acttcttcca cgccgtcttt
     3421 accggcgttt tgaatcaggt agttcaaacc ggcaatcgcg ctttcgccgc cttcgacacc
     3481 gaaacgtttc tgaccgacat atttggtatg cagataacgt tccaaagttt cggcagcagt
     3541 catctctttc aagatacggc gtttttgatc ggcattgtaa tgcggtgtgg acaatacgct
     3601 ttcaaaataa ttgcgtaccc agcggcgctc ttcggtattg ggaatataga tatattccaa
     3661 tgcgatgtgg ccgcagtagg tttgtttgag gttgctgatg atttgggaca aaggcagttt
     3721 gccgcgattg gcaaaatcgc cctcgcccat attgaattga agcgccatat cggcatctga
     3781 cagaccgtgg aatttcggat cgagggcttc aatatcgcgc ggggggatac gtttgagcgg
     3841 atcaagttgg gctgcaccca cgccttggat acgataggcg gaaatcagcc gtaaaacgct
     3901 gacttgcttt ttcagcattg cctcatccgc accgcccaca acggcagatg caattttctt
     3961 tttcgccaaa gtaacaaatg attcgcgaat cggtgtgtgt gcgacatcga cagcaaccgt
     4021 ccccggctgt ttgctcaaat cggtgaaata ctgcttccat ttttcatcaa ccgcatcggg
     4081 gttttccaaa aaagcctcgt acaattcctc aatgtagggt gcgttcgaac cgaacaggta
     4141 agagaaattg agtttttcgt ccatcatggc gtgcgctctt ttctttaaaa tataaacaaa
     4201 gggtagaagt ccgcgtcctg acggctaaac tgatgccgtc tgaaaagaca ttttattcta
     4261 ccctatttta aaccggattg cttgtcttga tttcatccga ctacaatcag acggattcgg
     4321 gaaacaatcc ggttctctga tattgcctgt ttgacaatat attaatgtat gttacctctc
     4381 gcctggcggc acataatcgc ggcgttccga accggtataa agctggcgcg ggcggccgat
     4441 tttcagcgaa ggatcgctaa tcatctcgtg ccagtgcgaa atccagccca cgctgcgcga
     4501 cagggcgaag atgacggtaa acatttcggt cgggatgccc agcgcggaca ggacgatgcc
     4561 ggaatagaaa tcgacgtttg gatacagttt gcgttcgata aagaacgggt ctttcagcgc
     4621 aatctgttcc aattccatcg ccagtttgaa tttcggactg tcttccaagc ccaattcctt
     4681 caaaacttca tagcaggttt cgcgcataat gctggcacgc ggatccatat tgcggtacac
     4741 gcggtgaccg aagcccatca gacggtattt gcgttgtttc acaccttcca tgtatgcggc
     4801 aacattagac acatcgccga tttcgtccaa catttttaag acggcttcgt tcgcgccacc
     4861 atgggatgca ccccacagac aggcgatacc ggcagcgata caggcaaatg ggtttgcacc
     4921 tgaagaacct gccaagcgta cggtcgaagt agaggcgttt tgctcgtggt cggcatgcaa
     4981 aataaagatg cggtcgagcg cgcgagccaa tactggatta ggtttgtatt cttcacatgg
     5041 tgtcgcaaac atcatgtgca agaagttttc agcataggaa aggttatttt ttggataatt
     5101 gaacggcaga ccgttggaat aacggtaaca cattgctgca attgtcggga ttttggagat
     5161 taagcggtaa atcgcaattt tgcggtgctc aggattggca atatccaagc tgtcttggta
     5221 gaacgcagac aaagcgccaa ccacgccaac catcatcgcc atcggatgcg catcgcgacg
     5281 gaacccccgg aagaaccaag tcagctgttc atgcaccatc gtgtggcggc ggacggtatt
     5341 gtcaaattct gccttttgct cgggagtcgg cagttcgccg taaatcaaca ggtagcagac
     5401 ttccaaataa tcggactttt cggccagctg ctcgatgggg tatccgcgat aataaagcaa
     5461 gccttgatcg ccgtcgatgt aagtaatttt agactcacag cttgcggttg aaacaaatcc
     5521 ggggtcgaag gaaaacaaac ctgtattttt tgtcagcccc cgaatgtcaa ccacatcgtg
     5581 cccgatgctg gcttccaata ccggcagctc caaacctgcc tgacccggta cgttgagttt
     5641 gattgatttg gacatattat tgctcctttg caagttataa cccacgttac tgcatccgca
     5701 gcacgttctt tgccttcaga cggcttttgc attttgaaat ctgcctgcag gcagatttca
     5761 gatgcctcat gcccgtctga ttttttcaag catcgggata aggtgccctt tgtccgtttc
     5821 cgaatgcccg ttaatcaagg caagcaattc ttgatcttga aattcaagga tttcggaaaa
     5881 ctcggacagc tctttatcgc tcaaatgctc gaattctttt tccataaacc tgccgaagat
     5941 taaatctaac tccaacaatc cccggcgggt ttgaaaacgg attttccgtt tggcaatatc
     6001 gtcaaaaacc atcatttctt aaacggcccg tttcaacata atctctttaa tcttaccgat
     6061 ggctcgggtc ggattcaagt gtttaggaca tacgtctacg cagttcataa tggtgtggca
     6121 acggaacaaa cggtatgggt cgttcagatt atccaaacgc tcattagtaa tggtatcacg
     6181 gctatccgca atgaaacggt aagcattcag caaaccagac gggccaacga atttatcagg
     6241 gttccaccag aatgacgggc aggcagtcga acagcaggcg cacaaaatac attcgtacaa
     6301 accgtccaac tctttacgct cttcctgagt ttgcagacgc tctttgtccg catcaatcgg
     6361 attatcgttg acaacataag gtttgacgga atggtattgt ttgaagaact gggtcatatc
     6421 cacaatcagg tcgcggataa caggcagacc tggcagagga cggattttaa ctggctgttt
     6481 caagccacgc agatcggtca aacacgccaa gccgtttttg ccgttgatgt tcataccgtc
     6541 agaaccgcaa atcccttcgc ggcaggagcg gcggaaagac aaggtatcgt cttgtgcttt
     6601 caggcgcact aaagcgtcca aaagtttcac gtcggtcggt tccaattcca actcgtaacg
     6661 ctgcatataa ggcttggcat caacatccgg gttgtaacgg taaatttcaa aactcatttt
     6721 ttccatgaga agtgttcctt ttctcatcaa ctgcccaatg attttgtatt ataaattcaa
     6781 gcagttcaat gtgggtatgg ttaggtttca gacggccttt gaggtaaagg ccgtctgaaa
     6841 acgcatcaat aaacgcgctt ggccggtttg atgtattcca cgctcaaggg cttggtatgc
     6901 accggtttgt aagacaaggt attggtatct gaatggtaca gcgtatgttt catccagttt
     6961 tcatcatcgc gctcaggatg gtcgtctgaa gcgtgcgcac cgcgtgattc tttacgtgct
     7021 tcggcagaca ccaaagtcgc tttcgccact tcaattaggt tatccaattc caaagcctcg
     7081 atacgcgcgg tattccacac tttgctcttg tctttgattt cggtacgttt cacacgctcg
     7141 gcaatcgcca tgacttctcg aacgcctttg ctcagaatct catcagtacg gaacacgccg
     7201 gcgtgcaatt gtacggagcg ttgcagttcg cggcgcaatg catcaacgtt ttcaccatcg
     7261 gtttggctgt ccaaacgctc gatacgttgg cgggtcaact caccagcatt agcaggcaaa
     7321 ggtttccagt cgctttgctc tttgatgaat ttaatcatgc tgtcgccggc agctttaccg
     7381 aataccacca agtccagcag ggagttcgta cccaaacggt tcgcaccgtg tacggaagca
     7441 caggcgcact cacctgcggc atacaggcct tttacaggca cttcgtattc gtctccttgc
     7501 ggcacaacga cttcgccgtg gtaattggtc ggaatgccgc ccatcatata gtgggtagtc
     7561 ggcacaacgg gaatcgggtc tttaatcgga tcgataccag cgaactgaat ggaaatctcg
     7621 cggatgcccg gcagtttttc cataattttt tctgcgccga tatggtcgat tttcagtaag
     7681 acatggtctt tgtttttacc gcaaccgcga ccttcgtaga tttccatcgc catcgcgcgg
     7741 gaaacaacgt cgcgagaagc caagtctttt acggtcggcg catagcgttc cataaagcgt
     7801 tcgccgtcgg cattcaacag aataccgccc tcgccgcgta cgccttcggt aatcaacacg
     7861 cccgcacctg ccacgccggt cgggtggaat tgccagaatt ccatgtcttc caacgggata
     7921 cctgcacgcg cacaaatacc caaaccatcg ccggtattca tataggcatt ggtagaagac
     7981 gcataaatac ggccgccgcc gccggtagca aacatcacag ctttagcgtg gaaaatataa
     8041 acttcgccgg tttccatttc catggcggtt acgccgacga catcgccgtt ttcatcacga
     8101 atcaaatctt gtgccgtcca ttccacaaag aattgcgtat tggcacggac gttttgttgg
     8161 tacaaagtat gcagcatcgc atgacctgta cggtcggcaa ccgcacaggc gcgttctacc
     8221 gcgcgtttac cgtgttcggc agtatggccg ccgaaaggac gctgataaat tttaccgctt
     8281 tccacacggt caaaaggcat acccatgtgt tccaactcaa ttacggcttc aggcgcggcg
     8341 cggcacataa actcaatcgc atcttggtcg cccaaccagt cggaaccttt cacggtatcg
     8401 tacatgtgcc agtcccaacg gtcttcctgc acattaccca gagaggcgga aataccgccc
     8461 tgcgccgcta cggtatgcga acgggtcggg aacactttag acaaaacggc acaattcaga
     8521 ccggatttgg ataattggag ggctgcgcgt aaacctgcac caccaccgcc gacaatcacg
     8581 gcatcaaact tgcgaacagg aaaacccata cttaccccca aattacttta attgaataca
     8641 ccaagcagcc gaccagccag acgatggtgg caacctgcaa aaacaaacgc acgccgaagg
     8701 gtttgatata gtccatccac aaatcgcgga tacccaccca agcgtgcaag aatacggcga
     8761 tgaagctcac ttgggtaaat acttttaccc aagtttgact aaaaaatgcc tgccatgccg
     8821 aatattcttt aggcagggaa aatagaacca ctaaaagtgc aacggtataa atcaacataa
     8881 taaccgcagt cgcacgttgc atcgcccaat cgcgcaaacc gtaatgggca ccggtcaatt
     8941 tacgttctac cataacaacg ctcccaaaac gacagtcaaa accaatgcag aagcaaatac
     9001 ggctttagcg gtattgcgcg cagtattcag ctcaaggcct ttgtgcgcat ccaaaaataa
     9061 aaagcggata ccggcgagag aatggtgcag ataagcccac aatacaccga ttaaaaccag
     9121 cttgaccaaa ggatgggaaa caatggcacg gtaagtttca aatgcagact cttgactcag
     9181 ggtaccggac aggaaataca gcaggaaagg cagcataata aacagcccga ccccactgat
     9241 gcggtgaagg atggaaacta tcccgggtat cggcagacgg atgttcggca aatccagata
     9301 aacaggacgc ggtttggcag acatagtcaa ttcctctgta gttatatatc gactcaaaaa
     9361 ccgtaaaccc attgctacac tgggttacat aaacgttaat ttacctgttt tatcccattt
     9421 tgaacagtgt attttaaaaa caaactgata gattttgaca aacacgcgtg gaaacagcag
     9481 attaaaagaa agatttttag ttttgccgcc cccttcccga cacggtatca gcatccgacc
     9541 ccatccccaa aaacaaaatg ccgtctgaaa aaatttcaga cggcattgtt ttgccatatc
     9601 ggtcaggctt tcaatttcgc atcggcatcg cgcaacgcgg tacgcagacc ttcctcgata
     9661 acgggatggt agaacggcat atccagcatt tgcggaacgg tcatcttcat ttgatgcgcc
     9721 catgccaaca agtgcgccaa atgttcggcg gcagggccta cgatttccgc gccgataaag
     9781 cggccggtgg ctttttcggc atacaggcgc atatggcctt tgtttaccag catcacgcgg
     9841 ctgcggcctt ggtttttgaa cgatacttcg ccgatgacaa attcgtcggc ttggtattgc
     9901 gcggcaacct gcgcgtattt cagaccgaca aagccgattt gcggactggt aaacaccacg
     9961 ccgatggtgc tgcggcgcaa accgccgccg atattcgggt agcggctcgc gttatcgccg
    10021 gcaatcttgc cttggtcggc agcttcatgc agcagaggca gttggttgga cgcatcgcct
    10081 gcgatgaaga tatgcggaat actggtctgc atggtcagcg gatcagcaac aggtacgccg
    10141 cgcgcatctt tttcgatatt gatattttcc aaaccgatat tgtcaacgtt cggacggcgg
    10201 cccacggctg ccagcatata ttcggcaaca aatacgcctt tttcgccatc ctgctcccaa
    10261 tggacttcta cattgccgtc tgcatcgagc ttgacctcgg ttttagcatc caaatgcagt
    10321 ttcaattctt cgccgaacac ggctttcgcc tcgtctgaaa cgacggggtc ggaaatgccg
    10381 ccgatgattc cgcccaaacc gaaaatttca actttcacgc ccaaacggtg caatgcctga
    10441 cccagttcca aaccgataac acccggcccg aacacggcaa cgcgcttagg cagcgtatcc
    10501 catgagaaaa cgtcatcgtt gataatcaaa cgattgccca aagactgcca ttgcggcagg
    10561 atgacgggac gcgaaccggt agcaatcacg aaactttttg ccgtaatttg agtatgctcg
    10621 tcgatttgga cggtatgctc gtcgataaat ttagccgaac ccatgatgcg cttgtcggca
    10681 ggccactctt ccacatcggc aacgacaaag ccgacaaaac ggtcacgctc ggatttaacg
    10741 cgctgcatga cctcttcacc gttgacgacg atgctgtctt tgtccaaatg cacgccgaac
    10801 gagtcggtat gcaatgcgtg gtggcgcgct tctgcggcgg caatcaagag tttggaaggc
    10861 atacaaccca cgcgcgcgca ggttgtgccg aacacattgt tttcaatcag gtaaacatta
    10921 tccgaatgta aacgggcatt gcgaaacgca cccataccgg cagtaccgcc gccgagtacg
    10981 acgacatccg cttgaatttt tttcataatc tttgtccttc cgttttcgtc tgaaacattg
    11041 ccgacacaga acatatcggt ttcagacggc atttaaatat tttcccaatc aaaaacatca
    11101 ttcagataaa taaaaacaaa atttcgattg agtgcaacaa tatccaaatt ctcttataaa
    11161 tatagagtta ttcggagcgg cagtgtagaa ccgcccgaat aactctatcc ggtatatcag
    11221 acagtttaat caaaccgcct taagcaaaca gctatcagtt tttagccaag taagcttcca
    11281 aatcttcgct gccgccgatg tatttgccgc cgatgaagac ttgaggggca gtcatcttgc
    11341 cggtaatggc gcgaacggaa gtaacggttg catctttgcc caatacgatt tcttcgtaag
    11401 acaaaccttt gtcttgcaaa gcttgtttgg ctttagcgca gaattggcaa cctggtttag
    11461 tgaaaattgc cacagactct tgagccttcc aatcgggagc aacgaattgc agcatagtat
    11521 ctgcatcgga tactttgaac ggatcgcccg gttcttcagg ttcgatgaac attttttcaa
    11581 ccacgccgtc gttaaccagc atggagtaac gccaagagcg tttaccgaag cccaagtctt
    11641 ctttaccgac cagcataccc ataccttcgg taaattcgcc gttgccatca ggaatcatgt
    11701 agatgttgtc ggattcttct tcggcagccc aagcgttcat tacgaacgta tcgtttacag
    11761 atacgcagta gattgcgtca acgccgtttt ctttgaacgc gccgaacaat tcgttgtaac
    11821 gcggcaggtg tgaagaagaa caagtcgggg taaatgcacc gggcagggag aatacgacta
    11881 cttttttgcc tttgaacaaa tcatcggtag acacatcttt ccaagtgtcg ccgacgcggg
    11941 tgcggaatac tacggaaggt actttttgac cggtacgatc ttgcaaagcc attttaagct
    12001 ccttaataga ttggtttgag aaataattgt gaatcgctgg gcattctacc ccatgcaaaa
    12061 tgatttgtat aatttatagt tcaaataata taaattattt aaaactatca aacacaactc
    12121 cgatttgcct gtttcccaca tataaagccg aatgagaaaa aaacaagctg caatgaaaag
    12181 attttccggc aactgcgccc ttgcgccgca aatcaggaca aaataccgtc tgaaaggctt
    12241 tcagacggta tttattcata cggtctcggc ttatttcgcc aattcggcac gcagttttcg
    12301 ggttacgttc atcatcactt ggagttgttc cagagtttct ttccagccac gtgttttcag
    12361 gccgcagtca ggattcaccc acagacgctc aaccggtaca acctcgatgg ctttgcgcaa
    12421 caggtgctca acttcggctt cagtcggtac gcgcgggctg tggatgtcgt aaacccccgg
    12481 gccgatgtcg ttcgggtatt tgaattcgcc gaacgcggtc aagagttcca tatcggaacg
    12541 tgaagtttcg atggtgatga cgtcagcatc catagcggcg atggccggca agatgtcgtt
    12601 gaactcggag taacacatat gggtgtggat ctgagtgctg tcttcacaac cggcagagga
    12661 caggcggaaa gattcgcccg cccagttcag gtaggcatcc caatcggcac gtttcaaagg
    12721 cagaccttcg cggatggcag gttcgtcgat ttgaatcact ttaataccgg ctttttccaa
    12781 atccaatact tcgtcattta aggccaaggc gatttgtttg cacacggtgc tgcgcggaat
    12841 atcgttgcgc acaaatgacc attgcagaat cgttaccgga ccggtcaaca tgcctttcat
    12901 cgggcgtttg gtcagggttt gggcgtaggt tgaccaagcc acagtcatcg cgttcgggcg
    12961 gctaacatcg ccgtaaatca ccggtggttt cacgcagcgt gagccatagc tttgtaccca
    13021 gccgtattgg gtaaaggcaa agccttccag caattcgccg aaatattcca ccatgtcgtt
    13081 acgctcggct tcgccgtgta ccaacacatc caaattgagt ttttcttgct cttcaatcac
    13141 caaggcgatt tcttttttca tcgcggcttc gtaatcagcg tcagacaatt cgcctttttt
    13201 gaacgcggca cgcgcttggc ggatttcagt ggtttgcggg aacgaaccga tattggtggt
    13261 cggcagcaac ggcaggttca gccatgcttg ttgcgcttta atgcgctcgg caaacggtga
    13321 tttgcgttgg tcggcatctg ccggtaaatc agccaagcgt ttggccactt cggcgcggtg
    13381 gatttcggtg ctgttggcgc gtgaatcagc agcggcttgg ctgtcggcca attcggcggc
    13441 aacggcttcg cggccttggt tcaatgcagt tttcaacacg cgcaattctt gcgttttctg
    13501 caaggtgaaa gccaaccatg agaacaaggc aggtttgttg actttcagct tttcttctac
    13561 cgacaaatcg tatggcgtgt gcagcagcga gcatgagctg gaaatccaca agcggtcgcc
    13621 caatttggct ttcaacggct caagcgtggc taatacgttg tttaaattgg cgcgccaaat
    13681 attacggcca tcaatcacac cggcagacaa cactttgccg taatcagcaa atgcgtccaa
    13741 ttgctcgggc gcgcgcacca aatcgatgtg caaaccggcc acaggcaagg ctttcagacg
    13801 acctgcgtgt gcggcaaccg aaccgaaata agtgctcaac aagattttgg cattcacttg
    13861 gctcaaagtg gcgtaaacgt cttggtacgc atccaaccac gcttgcggca ggtcaaccgc
    13921 caatgctggc tcatcgattt gaatccattc ggcaccggca gcggccaatt cggtcaggat
    13981 ttcaacataa accggcaata attttggcaa caggctcaag cggtcaaatt cgacagcgcc
    14041 tttttcttta ccaacccaca ggaaggtcaa cggaccaaca acggtcggtt tggctttcag
    14101 gcccaaagct tgggcttctt gcagctgttg aacgtagtgt ttggcgttcg ctttgaattc
    14161 ggtatcggcg tggaattcag gcaccaagta gtggtagttg gtgtcgaacc atttggtcat
    14221 ttcgatagcg aattggtctt tgttaccgcg cgccagttgg aagaattgtt ccaaagacag
    14281 gttttggctg tcgaagccga agcgggcggg aatcgcgccg gtggcgactt gcaggtcgag
    14341 gatgtggtcg tagaaagtga aatcgcctgc ggcaacgtaa tcggcgttgg cagcagcttg
    14401 gtgtttccag tttttctcgc gcaagtcttt agcaacagcc agcaattctt gctcgctgat
    14461 ttctttgcgc cagtattttt cttgggcgaa tttcaattcg cggaaggcgc cgacacgcgg
    14521 gaagcctgag aaatgtaatg ttgtcatgtt gaactctcca gttggatttt gtaaggaccg
    14581 gttcaatccg aatcggccgg tattgtaaat cagaatggct cggatggggc ggtgttttgc
    14641 tttttagaca ccttagatgc cgtctgaaac tgtttgatac ggctttaagg gcgcacgcct
    14701 aaaatatggc aaatggcgta agtcagctcg ctgcggttaa gcgtatagaa gtggaaatct
    14761 ttcacgcctt cgcgggacag gactttgacc atatcgatgg cgatacttgc cgccaccaga
    14821 ttgcgcgtac cttggtcgtc gtccaaacct tcatacattt gcgacagcca gcttgggatt
    14881 ttgacgttgg ttacttgagc cattttaccg agctgcttga agttggtaac aggcaaaata
    14941 ccggggacga tttccacatc gatacccaac atcacgcagc ggtcgcggaa gcgcaggtag
    15001 cgttctacgt caaagaaaaa ttgggtgatg acgtggttcg cgcccgcatc gattttgcgt
    15061 ttcaaattaa tcaggtcggc ttgtgcggat ttcgcttcgg gatgcacttc gggatatgct
    15121 gctacagaga tgtcgaagtc ggcgacggag cgtaatagct taaccaagtc ttcggcataa
    15181 aacggttttt tctcgtaacc gggcggctcg tcgccgcgca gggcgacaat gcggcggatg
    15241 ccgctgtccc aatagtcttt ggcgatttgg cgcaattcgt cgggagaagc gtcgatgccg
    15301 gtcaggtgcg gcgcggcttc caagccggtt tcttgcttaa tgcgtttgac gatgccgtgc
    15361 gtgcggtcgc gttcgccgga atttgcgccg taggttacgg atacgaactt gggatgcagg
    15421 gtttgcagac ggtggatgga atcccacagc atcgtttcca tttgttcgtt tttcggcggg
    15481 aaaaattcaa acgaaacgtt aatgtcgcct ttcaaatcag aaagactgtt atttaacgca
    15541 ttgatttctt ttgcgtaatt catggctgcg taccttttat tatcttttat atgatgtttc
    15601 aggcaacata ataaagttgc gccaaacatg agtcaatttc aaaattttca gatttatcat
    15661 gaaaatttct aatatattac aaaaatagtt aaatttcctt gtacaagcct tctaatttat
    15721 gataaagttt gttatgttat aacaattcaa ctaaggcgat atatgaaacc gaatattcat
    15781 cctgacaatt atcgaaccgt cctttttttc gacagcagcg ccaatgaagg ctggttgatt
    15841 cgttcttgcg ccgaaacaca cggaaaaacg atggtttgga cagacggcaa agaatatccg
    15901 ctgttttcgc tggatacttc ttccgcatcc catcccgtct atactggcaa acagcgcaac
    15961 gtcaacaccg aaggccgtgc cagcaaattc aaccaacgtt tccaatccgt catgtcatcg
    16021 tttagaaagg acaaataatg caggttctgt cttcactcaa aactgccaaa caacgccacc
    16081 gcgactgcca aatcgtcaga cgcaggggca aagtttacgt catctgcaaa agcaatccgc
    16141 gttttaaagc gcgccaacgc tgacccccta ccctttcctg cttcggttgt aaagcccctc
    16201 ctaccgcaag cccttttcaa atatgccgtc tgaaacagaa aaatgcgttt cagacggcat
    16261 tttttccgtc aatacgaatc cggaatgtgt ttgaaataca gcataaacag ataccaaaac
    16321 agtgtcgcgg cagctgcgcc aagcagtacc catgcgctga ttttggcgac atcgcgcagg
    16381 gcacgttttt gcttgcggct gaagaggcgg ttgataatca gcgcggcaag tgcgaaaaag
    16441 aaaaaaatac ggaaaagtct gcctatcata tagggaagcg gtggaggtcg tctgaaaaag
    16501 tatggatcgg ttgcggcggc atggggacgg atgtttcagc gcgtcgggct gcttgtccga
    16561 ccgtgtcaaa aagtcatgcc gtttgctata taataatgct tttcgccgca tttgttggaa
    16621 caggatatga gtcaaaatgc ctcggtgggc attgtaacgc cccaaaaaat tccgtttgag
    16681 gtgccgctgg ttttggaaaa cggtaaaact ttgccgcgtt tcgatctgat gattgaaacc
    16741 tacggcgagc tgaatgctga aaaaaacaat gcggttttaa tctgccacgc gctgtcgggc
    16801 aaccatcatg ttgcgggcag gcattcggcg gaggataaat atacgggctg gtgggacaat
    16861 atggtcggac ccggcaaacc gattgatacg gaacgttttt tcgtgatcgg tttgaacaat
    16921 ctgggcggct gtgacggcag cagcggacct ttgtcgatca atcctgaaac gggtagggaa
    16981 tacggcgcgg attttccggt ggttacggtg aaggactggg taaaatcaca agccgcgctt
    17041 gccgactatc tcggcatcgg gcaatgggcg gcggttgtcg gcggcagttt gggcggtatg
    17101 caggctttgc agtgggcgat ttcctatccc gaacgcgtgc gccatgcctt agtgattgcg
    17161 tctgcgccga aactgtccac gcaaaatatc gcgttcaacg atgtggcgcg tcaggcgatt
    17221 ttgaccgacc ccgatttcaa cgaaggacat taccgcagcc ataataccgt tcctgcacgg
    17281 ggcttacgga ttgcccgcat gatggggcac attacctatc ttgccgaaga cggtttgggc
    17341 aaaaaattcg gacgcgattt gcgttccaac ggctatcaat acggttatgg cgttgaattt
    17401 gaagtagaat cctatctgcg ctatcagggc gacaaattcg tcgggcggtt tgatgccaac
    17461 acctatttgc tgatgaccaa agctttggac tatttcgatc cggcggcgga tttcggcaac
    17521 agcctgaccc gcgccgtgca ggatgtgcag gcaaaattct ttgtcgccag cttcagcacc
    17581 gattggcgtt tcgcgcccga acgttcgcac gaactggtca aggccctgat tgccgcccaa
    17641 aaatccgtgc agtatatcga agtcaaatcc gcacacgggc acgatgcctt tttaatggaa
    17701 gacgaagcct atatgcgtgc ggtcgccgcc tatatgaaca acgtttataa ggaatgtcag
    17761 caatgaacct gcgcgatgat ttgcaactga tttacgaccg gatacccgaa ggcagccgcg
    17821 tcttggactt gggctgcggc gacggcgaac tgcttgccgc cttggtcgaa cacaaaaaat
    17881 gcagcggcta cggcatcgaa atcgacaccg acagcgtgat tgccgcgatg tcgcgcggcg
    17941 tgaatgttat ccaagccgat ttggaagaag gtttaaccgc attcaacgat caaagttttg
    18001 atgtgattgt gttgagccaa accatccaag cgatgcagaa taccgaaaaa atcctgcgct
    18061 gcctgatgcg cgttgccaaa caggcgattg tcagcttccc gaatttcggc tactggcgca
    18121 accgcgtcca aatcgcactc ggcggacata tgccggtttc cgaacgtatg ccctaccatt
    18181 ggtatgatac gccgaacatc cattggtgta cgctcaaaga ctttgattta ttgtgcgcca
    18241 aaaacaacat ccgcatcctc gagcgcgccg tgatgacggg caacagacag gtcaaacatt
    18301 tccccaacct tttgggcagt cttgcatttt accgcgtcgg gtaagacaga ttgagaacct
    18361 tgtcggcatt aacaggttct tcggttctat taacaacagg ccgtctgaaa aatgaaacgg
    18421 taggttttca gacggcctct ttaatactgg aaacaataga atgaaaaaca aaacctcatc
    18481 acttctctta tggcttgccg caatgatgct gaccgcgtgt tccccgagca aagaagataa
    18541 aacgaaagaa aacggcgcat ccgccgcctc gtccacggca tccgccgctt cgtcttccgc
    18601 gccccaaacc gatttgcaac cggccgcatc cgcccctgat aacgtcaagc aggcagaaag
    18661 cgtgccgccg tcaaattgca ccgacctgca ccccgccacc ggcattgacg atctcatgca
    18721 gcaaatcgcc gaacacattg actcggactg tctgtttgcc ctttcccatc acgaactgga
    18781 aacccgtttc ggcttacccg gcggcggcta tgacaacata cagcggctgc tgtttcccga
    18841 catccgccct gaagatcccg actaccatca gaaaatcata ctggcaattg aagacttgcg
    18901 ttacggaaag cgcacgatca gccggcaggc acaagatgcc ttgatggaac aggaacgccg
    18961 cctccgagaa gcgacgctgt tgctgataca gggcagtcaa gaaacccgcg gacaaggcga
    19021 ggagccgaaa cgcacgcgtt attttgaagt ttcggcaacc cctgcctatt cgagccggca
    19081 caacaacgga cttggcggca atttccaata catcggccaa ttgcccggct atctgaaaat
    19141 acacggagaa atgcttgaaa accaatcact cttccggctg tccaaccgtg aacgcaatcc
    19201 cgacaaaccg tttttagaca tccattttga cgaaaatggc aaaatcacgc gtattgtcgt
    19261 ttacgaaaaa aacatctact tcaatccaaa cttggggcga agataaaaaa atgccgtctg
    19321 aaacgttttc agacggcatt tttcaatgca acaaatcaag cgcgtttgcg gaattcgccg
    19381 gtgcgggtat cgatttcgac tttgtcgccg ttttcgatgt aagacatcac ttggatttcg
    19441 gtgccgccga ccaggcgcgc ggttttcatc actttgccgg aagtgtcgcc tttgacggca
    19501 ggctcggtgt actcgacttc gcgcacgatg atggtgggca gttcgacaga aatggggttg
    19561 ccttcgtaga aggtaacttc gcattggtct tccataccgt caacgatgaa tttcaacgcg
    19621 tcgccgatgt tgtcggcttc gatttcgtat tggttgaatt cttcatccat aaagacgtac
    19681 atcgggtcgg caaagtagct gtacgtacag tttttgcggg acaggatgac cacgtcgaat
    19741 ttgtcgtcgg ctttgtaaat ggtttcggaa gccgcgccgg tcagcaggtt tttcagtttc
    19801 atgctgactt tggcggaaga gcggccgcct ttgatgtatt cggttttttg aacgaccata
    19861 ggatcgttgc cgaccataaa tacattgccg gcgcgcagtt cttgtgctgt tttcattgtg
    19921 attttccaat caaatcggtt gtcgtaaaac gcgctattct agcgtatttt ttgatgcttt
    19981 gaaacaaagg cggcgagttt ttcggatgcg gaaggctgcc cgaaaagata acggctccaa
    20041 tcttccgcgc cttgccgcca gccgttttga tgttgttgca gggtttgcca acattcgagg
    20101 cgttgcgtgg cggataaagc ccctccgccg ttgaggtcgt ctgaaaggcg gcggtgtgcc
    20161 gatgcggttt cgggcgtgta gaaaccgtgt gccttatccc aaaaggcgtg gagtttgtcg
    20221 agatggacat tctcatcttg cgggtagatg tgccaaaaga agggtttgcc cgcaagctgg
    20281 gcgcgcacga aactgtcttc gccgcggatg acggcgcaat cggcgaggtg cagcagtttg
    20341 tcgaagtcct gttgcggcac gaaagggatt ttgacaaggc agacggatgc cgtctgaaaa
    20401 acatcgccgt cgttttgcag ggcgttttgc ggaatgatgc cgctttgttt gaggctgtcg
    20461 ataatttgcg cccccgccag caacagtgtc aacggactgc ctgcctgttg ccacatttcc
    20521 agccactttg cccaaacatc gctccgatag ccgaaaagca gccattcgga ggcgtttttt
    20581 tcgggcagca tcagccgctc tcgcagggct tcagtatcga aacggacggc atctcggtaa
    20641 tcacgttcgc gtatcaaccc gccgcttttt tcgctgaaac ccataaacca aaaatatttt
    20701 tgaacaccct cctgcggcga aggcatcagg tgcagccttt cattgctttc ctccgcgctc
    20761 aaatattccc aattcagcca aagcggcttg tgtcggcgga taatgtgcag cacattttcg
    20821 ggcaggtcgc aggcaaaagt ttcgatgacg gcatcgggaa caggcgcggt atcaatatct
    20881 gccgcatcgg aatgccaagt gcggacatga atatcctgat gaacgcaggg aacatcgggc
    20941 aaatcagggc aaagcgcacg caaggcggac acatcgtccg tccacaaatg cacctgccaa
    21001 ccgagttcgc ggtgcaaaac acgggcaagc cgccacgaaa cgccgatgtc gccgaaattg
    21061 tcgatgacct tgcaaaaaat ccagcagaca aaaggaggag tattcataga aataacggaa
    21121 acgggctaaa acgcgccaaa atagtggaac aaatacacaa tcagcaaaat aatcggaata
    21181 aacagcaaca tagtttgacg tttcaaatca aactcttgtt tcaaatgatg cttgatggtg
    21241 tcgaaaagga cgaatttaaa caaaaacgtc gcttcgcagg caaggatgat gccgttaatc
    21301 agatgctggg gtttgtcttc gggcggattg gcgacgaaaa cggcaagcgt cgccaagggt
    21361 atcaggcaga cgacaagcca gcggacaagc aggtttcggg acattgcaag gctttcaaaa
    21421 aagcaaaacg ccattatcgc cccaagcgca cgccttgtct aacggatgcg ccggagatgc
    21481 cgtctgaagc cttgccgccg gaaaacgcag tgcttccgca aaccaaaata taacgtcggc
    21541 acgctttccg ctataatccg cattttcgca accctgaaac atcatgccga ccccgaaagc
    21601 ctttgccctg ctcgggccga ccgccggagg caaaaccgcg ctcgccctca aaattgccga
    21661 aaccctgcct gtcgaaatca tcagcctcga ttccgcgctg gtttaccgcg atatggacat
    21721 cggcacggcg aaaccgtccg cctccgaacg cgcctttgtc ccgcaccacc ttatcgacat
    21781 catcccgcct accgaatcat acagcgccgc ccgctttgtc gaagactgca cgcgcttggt
    21841 cggggaaatt tcctcacgcg gcaggtttgc ccttattgtc ggcggcacga tgatgtattt
    21901 ccgcgccctg acccaaggtt tgaacgattt gcccgaagcc gacgcctgcc tgcgtgccga
    21961 cttggacgaa caaaaacaga tgtacggctt ggatttcctc taccgcaccc tgcaaaaagt
    22021 cgaccctgaa accgcatgcc gtctgaaacc gaacgacagc cagcgtatcg aacgcgcttt
    22081 ggaagtttat tatctgaccg gcaaaccgat gagcgaacac ctcggcagac aaagccccca
    22141 tacgctcccc ttcgatttgc ataccgccgc cctgattccc gaaaaccgcg cccgcctgca
    22201 tgaaaatatc gccctgcgtt tccacctgat gcttgaacag ggctttatcg gcgaagtaga
    22261 aaacctgcgc cgccgctatc ccggcctgac cgccgactcc cccgccatcc gctgcgtcgg
    22321 ctaccgtcag gcatggaaat atctggatgg aaaaaccgat ttcccggcat ttgtcgagaa
    22381 aggcattgcc gccacccgcc aacttgccaa acgccaactg acttggttgc gcaaaacacc
    22441 tttagactgc gttgccgacc cgttttcaga cggcacttca tgcacacgcc tgattgaggc
    22501 ggcaaaacgg tttttcggcg cataaaccat gctgaccaca ccgccgctcg cccccaaaac
    22561 cgtcgccgcc ctgcacaggc tcggtatccg tacactcgaa gaactgcggc aaaacggttc
    22621 ggtcaaagcc tttctgctgc tcaaagcatc gggtttgacc ctcacgaaaa gcacactgtg
    22681 gcaactcgag tcgctgctga acggcacgcc gccgcaagaa atgtcccaag cgcataaagc
    22741 ccgcctgctt gccgagttaa aaaaccatcc gcccgtcgcc gcctttccgc cgcaggaaga
    22801 aatggaacac ttcatgtgcg aagcactgcg ccaagcagaa caatcctccg ccgacggcga
    22861 aatacccgtc ggggcagtca tcgtttcaga cggcaaaatc atcgcatcgg cacacaacac
    22921 ctgcattgcc gactgcaatg tcagccgaca cgccgaaatc aacgccttgg cacaggcagg
    22981 cagagaaata caaaactacc gccttgacgg atgcgacata tatatcaccc tcgaaccctg
    23041 cgccatgtgc gcgtccgcac tgatacaggc acgaatcagg cgtgtgatat atggtgcggc
    23101 agaacccaaa accggagcgg caggcagcat tgtcaacctg tttgccgaca aacgcctcaa
    23161 tacgcacacc gccatacggg gcggaatcct gcaagaagaa tgccgcgccg tattaagccg
    23221 ttttttccaa aacaaaagaa aaggttgaac ctatgaccgc cttgctcgtc atcctcgccc
    23281 tcgccctgat agccgccggt acggcgggca tcgtttaccc cgccctgccc ggattggcat
    23341 tgatgtttgc cggaacctgg ctgctcgcct actccggcgg ctaccaaatc tacggcgcgg
    23401 gcgttttgtg gacggtcgga ctcatcagcc ttgccggcat actggcggac tatgtggcag
    23461 gcatatgggg gacaaaatat accggagcgg gcaagctcgc cgttcgcggc gcattggccg
    23521 gcagcatcat cggcatattt ttctcccttc ccggactaat actcggcccc tttatcggcg
    23581 cggcggcagg cgaactgatc gatcggcgca atatgcttca ggcaggtaaa gcgggcttgg
    23641 gtacactgtt gggacttgtc gtcggcacgg cgttcaaaat cggctgcgcc gtatccatct
    23701 tgtttatcct gttggtgaaa tacatcgcat acctgtttta accccttcag acggcatcag
    23761 gataaattgc tataatgccg tctgaaattt ccgacaaagc accatcatgg caaaccaaag
    23821 acccatctac ggcttccacg ccgtcaatgc ccgattgtgg caaaacccca aatccatcgt
    23881 cgaactctac atccaagaag gcaaatcaga cgcacgcacg cgcgaagtgt tggaaaaggc
    23941 ggcaaacgaa aacatccgcg tatattttgc cgatgccgac cgcctcaacg ccatcagcaa
    24001 aggcgcgcgc catcagggtg tggccggatt tatcgatgcc tccaaaaacc acgtccatct
    24061 cgaagacgta ttggaaaact taagcgaacc gccgctgctg ctgatactcg acggcatcac
    24121 cgatccgcac aacctcggcg cgtgcctgcg taccgccgac gcaatgggcg tacacgccgt
    24181 catcgcaccg aaagacaaaa gcgcggggct gaacgccacc gtcagcaaag tcgcctgcgg
    24241 tgcggcggaa accgtcccct acatcaccgt aaccaacctc gcccgcaccc tacgcgaatt
    24301 gaaagaatac ggcatttgga tcatcggcac cgatatgggc ggcaacgccg acctttacca
    24361 ttgcgatctg cccgacagcg cggcatgggt gatgggcaac gaaggcgacg gtatgcgccg
    24421 cctgacacgc gaacattgcg acatgctggt gtccataccc atgttcggca cggtcgaaag
    24481 catgaacgtc tccgtgtctg ccggaatggt attaagtgaa acacgccgac aacgcgtatt
    24541 aaaaaacgaa aaagcgtaag cctcaaaaac aatgccgtct gaaacaggga ttccgtttca
    24601 gacggcattc attttcaaaa tcagtgttcc aagtggtcgg tatcaaactt gaccgccgcc
    24661 tcggttttat cttcgggcaa gaccaaattc agcaagactg ccgtaatgcc gccggcggaa
    24721 atagagtttt ggaacaagac gggcaggttt ttaaacactt ccggctcaaa cgcgacaccc
    24781 aagcccaaac cgaccgacgt tgctgcaatt accgcttcgc gcctgcggat gccgtggctg
    24841 accaaaatcc gcacgcccgc aatcgcaatc aagccgaaca tcaaaaccat cgcgccgccc
    24901 aacaccggac tcggaatcgt cgtaaacgcg cgaccgacaa cggggaacag acccaacagc
    24961 accaaaatca cggcaatata tttgcccaca tggcgcgaag ccacgccggt catctgaatc
    25021 acgccgttgt tttgcgcaaa cgttgtcagc ggcagcgaac ccaaagccgt cgcaatcacc
    25081 gacaccaagc cgtctgccaa cacgccgccg cgcaggcgtt tggtgtattc ctcgccttca
    25141 atcggctggt cggacaccat tgccgtcgcg gttaaatcgc cgaccgcctc aaacacgctc
    25201 aacaagaaaa tcgcgcccgc cacaataaac gcgtgccagt cgaaagcaaa accgtattta
    25261 aacggtacgg gcagcgtaac cagcggcagg ttttgcagcg cggaaaaatc caccttgccc
    25321 aaaaacagcg cgacaatgta accggcaatc agaccgaccg cgatgccgct catccgcaac
    25381 agcgggtttt tcatgcagtt gaatatcaac acaatcagca acaccagcga agccagcccc
    25441 aggttttcca tcgagccgaa cgtgccgtcc gccttcgcgc cgaagccgcc gccgaaatcg
    25501 gtaataccga cgtgtaccaa actcaagcca atcagcatca cgaccacgcc actgaccgtc
    25561 ggcgtaatca cttttttcaa atacggcagc agccacgccg agaaacacac caaaaacgcg
    25621 ccgacaaacg atacacccaa gagcgtcgaa atcatcgcat ccttagtcaa accgccctct
    25681 ttcatccccg cgccgagcgc aatcatgacg gtaacgaacg agaaattcac cgactggatg
    25741 gacagcatcc ccgaaccgac cggcccgaag cggttgacct gcaaataagt gccgacaccc
    25801 gacgcaacca tcgccatcga cacgagatac gccgtcattt ccaccggcag ttccagcgcg
    25861 ccgcccacaa tcagcgcggg cgtaatcatc ggcacaaaaa tcgccaaaag atgggtaacg
    25921 gcactcaaga gcgcattacc gaacggcggc ctgtcttcca aaccgtacac caaatcaggc
    25981 gcatccgccc ctttgcccaa ctgaccgctc attgtatttc cttattttaa aggttgaaaa
    26041 atccaagcgc ggattgtagc gaatctgccg cacggcttca atttacattt cagacggcat
    26101 ccgtctgccc cacacgttta ccac
//
LOCUS       DAXHRI010000012        25119 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXHRI010000012 DAXHRI010000000
VERSION     DAXHRI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.012"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..439
                     /locus_tag="ACFM75_000361"
     CDS             <1..439
                     /locus_tag="ACFM75_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6254961.1"
                     /translation="DKQIDDHTRTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLS
                     HKRIASLAGIAPHPRESGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFYQRL
                     PSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
     gene            487..1560
                     /locus_tag="ACFM75_000362"
     CDS             487..1560
                     /locus_tag="ACFM75_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6254962.1"
                     /translation="MRPFCVGATVLAILGALVFFISPGVVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPATTLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAW
                     LIWLARNTDNFALLMLLAAFTVFQTAYAATGDLNLLRAQVHLNMAAVMFVSIRVSVLL
                     GAEALKECRLKDPVFIPNIVYKNIAITFLLLHTAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRTVLMNVNPIFFITVPAILTVA
                     VFVLYLFTFVPIFQANAFTGDPE"
     gene            complement(1607..3796)
                     /locus_tag="ACFM75_000363"
     CDS             complement(1607..3796)
                     /locus_tag="ACFM75_000363"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC6254963.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVRTAFSDEPLLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVGNRFADVPTAVLH
                     SQMAAGRRTQDYLRAMSGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRTFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESVSRQDLHRAVSLWVQVLQQNRDSKIRWSVDVDPQEA"
     gene            3935..4717
                     /locus_tag="ACFM75_000364"
     CDS             3935..4717
                     /locus_tag="ACFM75_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC6254964.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(5035..6969)
                     /locus_tag="ACFM75_000365"
     CDS             complement(5035..6969)
                     /locus_tag="ACFM75_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC6254965.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDDIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHKAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(7035..8213)
                     /locus_tag="ACFM75_000366"
     CDS             complement(7035..8213)
                     /locus_tag="ACFM75_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC6254966.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8439..8828
                     /locus_tag="ACFM75_000367"
     CDS             8439..8828
                     /locus_tag="ACFM75_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6254967.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFATYDKNKLKQNEYFKITLSEVSPDHFTLQAAPDPMTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            9111..10157
                     /gene="adhP"
                     /locus_tag="ACFM75_000368"
     CDS             9111..10157
                     /gene="adhP"
                     /locus_tag="ACFM75_000368"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC6254968.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVNADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(10322..13807)
                     /gene="smc"
                     /locus_tag="ACFM75_000369"
     CDS             complement(10322..13807)
                     /gene="smc"
                     /locus_tag="ACFM75_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC6254969.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELQAEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTRQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQNR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQASDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIVTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            13809..14060
                     /locus_tag="ACFM75_000370"
     CDS             13809..14060
                     /locus_tag="ACFM75_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254970.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHIFLAKPYRILRPRCRLKPSDGIFN
                     APEPRFRQCPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            14057..14779
                     /gene="lpxH"
                     /locus_tag="ACFM75_000371"
     CDS             14057..14779
                     /gene="lpxH"
                     /locus_tag="ACFM75_000371"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC6254971.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAAGQARAIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTERQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            15048..16634
                     /locus_tag="ACFM75_000372"
     CDS             15048..16634
                     /locus_tag="ACFM75_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC6254972.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            16672..16812
                     /locus_tag="ACFM75_000373"
     CDS             16672..16812
                     /locus_tag="ACFM75_000373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6254973.1"
                     /translation="METAVPRLGGASGRIPVPEQARPAKQMPSETEKGFRRHFSAVCV
                     QA"
     gene            complement(16803..17006)
                     /locus_tag="ACFM75_000374"
     CDS             complement(16803..17006)
                     /locus_tag="ACFM75_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC6254974.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPGNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            17122..17619
                     /locus_tag="ACFM75_000375"
     CDS             17122..17619
                     /locus_tag="ACFM75_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC6254975.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17701..18894
                     /locus_tag="ACFM75_000376"
     CDS             17701..18894
                     /locus_tag="ACFM75_000376"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC6254976.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQNFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(19298..20233)
                     /gene="hemC"
                     /locus_tag="ACFM75_000377"
     CDS             complement(19298..20233)
                     /gene="hemC"
                     /locus_tag="ACFM75_000377"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC6254977.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVILQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20352..20867
                     /gene="ybeY"
                     /locus_tag="ACFM75_000378"
     CDS             20352..20867
                     /gene="ybeY"
                     /locus_tag="ACFM75_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC6254978.1"
                     /translation="MKRAKQYPFLSLQRQRFHLNFENASSTANLPSERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            20869..21693
                     /locus_tag="ACFM75_000379"
     CDS             20869..21693
                     /locus_tag="ACFM75_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC6254979.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(21836..23215)
                     /gene="nhaC"
                     /locus_tag="ACFM75_000380"
     CDS             complement(21836..23215)
                     /gene="nhaC"
                     /locus_tag="ACFM75_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC6254980.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLVRGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23506..24729)
                     /locus_tag="ACFM75_000381"
     CDS             complement(23506..24729)
                     /locus_tag="ACFM75_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC6254981.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         5730 a         7135 c         6507 g         5747 t
ORIGIN      
        1 ggacaaacaa atcgacgacc acacccgcac gcattttgac ggcaaagccc aagtggcaga
       61 gcaaatcaaa ggcatcggtt cgataacgac ggctacgctg atggcgatgc tgcccgaatt
      121 ggggcggctg tcgcacaaac ggatagcgag tttggccggc attgccccgc acccgagaga
      181 gagcggggaa accaaattca aaagccgctg cttcggcgga aggtctgcgg tgcgtaaggc
      241 actgtatatg gctaccgtgg cagcgacacg ttttgaaccg cttattcggg atttctacca
      301 acgcctgccg tccgagggta agccgtataa ggttgccgtt acggcatgta tgcgtaaact
      361 gctgacgata tcgaatgccc ggatgcgtga ttattttgcc gaaaacgata ccgccgaaaa
      421 cggtatccgg acggcttgat ttgagttttg gtatttttgc ccgacggggt gaaaaatact
      481 gttgctatgc gcccgttttg cgtcggcgcg acagtgcttg ccatactcgg cgcgttggtg
      541 tttttcatca gccccggtgt cgtcgtcctg caccgccaaa tcttcttaga actcatgctg
      601 cctgcagcat atggtggttt cctgactgcg gctttgttgg actggacggg tttttcgggc
      661 aacctgaaac ctgccactac tttgatggcg gtgttgttgc ttgttgcggc tgttttattg
      721 ccgtttttac cgcaacttgc cgcattcttt gtagcggctt attggctggt tttattgctg
      781 ttctgcgctt ggttgatttg gctggcccgc aacacggata acttcgccct gctgatgctg
      841 cttgccgcgt tcaccgtttt tcagacggca tacgcggcaa ccggcgactt gaacctgttg
      901 cgcgcgcaag tgcatttaaa tatggctgcg gtgatgtttg tttccatacg tgtgagtgtt
      961 ttgctgggcg cggaagccct taaagaatgc cgtctgaaag accccgtatt catccccaac
     1021 atcgtctata aaaacatcgc catcaccttc ctgctgctgc acaccgccgc cgaactttgg
     1081 ctgcccgcgc agaccgccgg ttttaccgcg ctcgccgtcg gctttatcct gcttgccaaa
     1141 ctgcgcgaac tgcaccatca cgaactcctg cgcaaacact acgtccgcac ttattacctg
     1201 ctccagctct ttgccgccgc aggctatttg tggacaggcg cggcgaaact gcaaaactta
     1261 cctgcctccg cgcccctgca cctgattacc ctcggaggca tgatgggcgg cgtgatgatg
     1321 gtgtggctga ccgccggact gtggcacagc ggctttacca agctcgacta ccccaaactc
     1381 tgccgcatcg ccgtccccat ccttttcgcc gctgccgttt cgcgcactgt tttaatgaac
     1441 gtgaacccga tattcttcat caccgtcccc gcgattctga ccgtcgccgt gtttgtgctt
     1501 tatctcttta cgttcgtacc gatctttcag gcaaatgcgt ttacgggcga tccggaataa
     1561 aaatgccgtc tgaaacggtt ttcagacggc atcggattgc caataatcaa gcctcctgcg
     1621 gatcgacatc caccgaccat ctgattttgc tgtcgcggtt ttgctgcaac acctgcaccc
     1681 acaaactcac ggcgcggtgc aaatcctgtc gggataccga ttcgaggaag atttgcgcgc
     1741 gttcgcgttc ggcgaggcgc accatcagca tcggggccgc gccgaactgg gaaacgcttt
     1801 cgggcaacag cggggcgagg gtttctttgg cggcattgag aaattccatc gcatcggcaa
     1861 cgcgcggcgc atcggcgcgg acggcggtct ggaaaccgaa gggcggcatg gcgaacattt
     1921 gccgctcgtt caattcgttt tcggcaaaca cggcatagtc ttgcgctttg acggcggcga
     1981 agacaggatg ttcgggcagt tgggtctgta tcaacacctt gccgggtttg tcggcgcgcc
     2041 ccgccctgcc tgacacttgc atcagctcgg caaacagcct ttccggcgcg cgaaagtccg
     2101 cgctgtacag gctgccgtcg gcgttcaata cgataacgag gttgagccgc gcgaaatcat
     2161 ggcctttggc aagcatctgc gtgccgacca aaatgtcgat tttgttgtcg gcgatgcggc
     2221 ggtacaaatc cgcccagtca tttttgtgcg cggtgctgtc cctgtcaaca cggacgacgg
     2281 ctgccttggg caggaaggtg cgcagggttt cttcgacgcg ctgcgtgccg tggccgacgg
     2341 cggtcaggtc ttggttgccg cagtcggggc atttgtacgg gatgggttcg cggtggtcgc
     2401 agtggtgaca gcgcagttgg cgggcgcgtt ggtgcagtac cattttggcg gagcagtttg
     2461 ggcagccgaa ggtatggccg cagtcgccgc aaaacagcgc gggtgcgaag ccgcgccggt
     2521 tgaggtacac cagcgacata ccgcccgctt caaaattctg tttcaaaagc tgcaaggctt
     2581 gcggcgagaa accgttgtcg agtttcagac ggcctacgtt gagaatgtcc acttgcggca
     2641 gttgcgcggc ggtatgggcg cgttcggtca gttgcagcag gcggtacgcg ccgctttgcg
     2701 ccttgtgcca gctttccaag ctgggcgtgg cactgcccaa tatgatgggg cagccgccct
     2761 gcttcgcccg ccacaccgcc aaatcgcggg cgtggtagcg caattcgttg tcttgtttga
     2821 acgagccgtc gtgttcctca tcgactacaa tcagcccgac atcatccatc ggcgtaaaca
     2881 ccgccagccg cgtgccgatg accaatttcg cctgccccga catcgcgcgc aaataatcct
     2941 gcgtgcgcct gcctgccgcc atctgactgt gcaacacggc ggtcggcacg tcggcaaaac
     3001 gatttcccac ccgcttcaaa agctgcggcg tgaggttgat ttcgggcaac agaaacaaca
     3061 cctgccgccc ctgagccaac actttcgcca tcgcatcgaa atacacttcg gtcttgccgc
     3121 tgccggtgat gccgtacagc agaaacggct ggaagcgtcc caatgccgtc tgaatggcat
     3181 cggaagcctt ctgctggtca gcatttaata caaattcaga gtgcgaagcc tgcccattac
     3241 acgaccttaa aacgggcttc gccgcttccg tcgtttcaat ccaaccctgc tccgcccaat
     3301 cttcgattaa cttcgccgcc tgcgcgttta cctgcttcaa cgctgccatc gtcatttcgc
     3361 ccgacagcag cgcgtcccac aaagccgctt ttttgttgaa ccgcgccggc ggcggcgttt
     3421 gcgccctgcc cgcttcgttc aacgcgtaaa acaagggcgg ctgcggcatt tccaccgcgc
     3481 gcgtttcctt caaaccttgc ggcaatgcgg caaatactgc ctgcccggtc ggatagtggt
     3541 aataacgcga cgtaaacgag agcaaatcac gccagctttc gggcagcagc ggctcgtccg
     3601 aaaaggccgt ccgaacgctc aaaatccgcg ccgtatccat atccggcgca atatccgttt
     3661 cccacaccat cccgaccacg gttttattgc ggaaaggcac aagcacccgc gttcccggag
     3721 gaagcggatc ggaatgggaa taagtcaaaa ggccgtctga aagcggcacg tttacggcga
     3781 tgcggtggta gatcatacag gcaggcaaat catttttttg cgtattttaa caggcggaag
     3841 ctattttcag acgataaatt gagcctaggc gcaatgcagg cgcaacagag atttgataca
     3901 attccgcttt tccaacacgc gcaatcatct ttatatgaaa accaagttaa tcaaaatctt
     3961 gacccccttt accgtcctcc cactgctggc ttgcgggcaa acgcccgttt ccaatgccaa
     4021 cgccgaaccc gccgtcaaag ccgagtccgc cggcaaatcc gttgccgcct ctttgaaagc
     4081 gcgtttggaa aaaacctatt ccgcccaaga tttgaaagtg ttgagcgtca gcgaaacacc
     4141 ggtcaaaggc atttacgaag tcgtcgtcag cggcaggcag attatctaca ccgatgccga
     4201 aggcggctat atgttcgtcg gcgaactcat caacatcgac acgcgcaaaa acctgaccga
     4261 agaacgcgcc gccgatttga acaaaatcga cttcgcctcc ctgcctttgg acaaagccat
     4321 caaagaagtg cgcggcaacg gcaagctgaa agtcgccgtc ttttccgacc ccgattgtcc
     4381 gttctgcaaa cgcttggaac acgagtttga aaaaatgacc gacgtgacgg tttacagctt
     4441 tatgatgccc attgccggcc tgcaccccga tgccgcgcgc aaggcgcaaa tcttatggtg
     4501 tcagcccgac cgcgccaaag cgtggacgga ttggatgcgt aaaggcaaat tcccggtcgg
     4561 cggcagcatc tgcgacaatc ccgtcgcgga aaccacttcc ttgggcgaac aattcggctt
     4621 caacggcacg ccgaccctcg tcttccccaa cgggcgcagc caaagcggct acagcccgat
     4681 gccccaactg gaggaaatca tccgcaaaaa tcaataatcc atccgcaaaa caaaaggctt
     4741 ggaaaattcc aagccttttt tgcgggaaat gccgcctgaa aaccaatccg cactgaaagt
     4801 tatagtggat taaatttata gtggattaaa tttaaaccag tacagcaagc caacaaacac
     4861 atcctaaccc attgaatgat tgaaaataat aaattgaaca atacgaaaca caccgccgtc
     4921 attcccacgc aagtgggaat ttggaacgta gaatctaaga aaccgttttt gtccaataag
     4981 tttctgctcc aaaatgaccg gttctgcagc ttcaggcggc atctttgtcc aacctcaatc
     5041 ctgtgccaat gcgtctatcg gattgagttt ggctgcttta ttggcaggca taaagccgaa
     5101 cgcgatgccg attccggtcg agcaggcgac cgccccgata acggatgccg ccgaaatgtc
     5161 catcgggaag tcggttacaa aatgattgaa cacgaggctg acggcggcgg acaaacccac
     5221 gccgaccaaa ccgccgatga cgcagattaa caccgcctca atcaaaaact gctgcaaaat
     5281 attgccgcgc cgcgcgccga ttgccatccg tatgccgatt tctttggtgc gctcggtaac
     5341 ggacaccagc atgatgttca tcacgccgat gccgccgact accaatgaaa tcagggcgat
     5401 ggaggaaatc agcagcttca tcgtaccggt ggtgctttcg actatctgcc tgatgctgtc
     5461 gctgttgttc atgaagaaat cttccgtgcc gtgccgcgct ttgagcagat cggtcagccc
     5521 tttttcggca acctgagtat tggcattgtc tttgattttg acggtgatgg agttggtgtg
     5581 gctctcgcct gtgatttggt gcatcaccgt cgtatagggc gaccaaagca tcagcacgtc
     5641 ggaattgccg aaagcgtttt cgtctttttt catcacgccg atgacggtca aggggcgttt
     5701 cctgaacaaa atggttttac ccaacggatc cgagtccgca aagagtttgt ctttgacatt
     5761 ttggtcgatg acgacgacct gcgcgtcttc tttcacatcg ttttcgtcaa acagccgccc
     5821 cgtttccagc ttcagtccgc gcacgtcgaa atattgttcg cccacgccgt acaaagaagc
     5881 ggtcaggtcg gtattgcggt aagtcagcgt gccgccgctc gaagtcatgg gcgtggcgga
     5941 agcaacgtag ctttgtttgg cgatgatttt tgcgtcgtct atggtcaggg ttttaatcct
     6001 gccgctgcgc ctgtcgccga agccgcgccc cgggaagatg ctgatggtgt tcgtccctat
     6061 cgaactgatg tcttcaagga tttttttctg cgaaccgttg cccaatgcga cgacggaaac
     6121 caccgacgcg atgccgatga tgatgccgag catcgtcaga agcgaacgca ttttgtgcgc
     6181 caatactgct tgcaccgaca ttctgaaggc ttcgacaaac tggtcgtaat aaaacgacca
     6241 cgaggctttt tcccgaatcc tcccgacatt gcttgcgggg atttcgggat ttttcgaggt
     6301 gtcggaaatg atttcgccgt cccggatttc gatgacgcgg ttggcattgg cggcgatgcc
     6361 ggggtcgtgc gtgaccataa tgacggtatg cccggctttg tgcagcttgt ggatgatttc
     6421 catcacgttt ttgccgctgg cggtatcgag cgcaccggtc ggctcgtcgg cgaagatgat
     6481 ttctccgccg ttcatcaggg cgcgggcgat ggagacgcgc tgctgctgtc cgcccgagag
     6541 ttcgccgggc ttgttgccct ctttgcttgc caaacccaaa tcctgcaaca acttctccgc
     6601 acgcgcggaa cgctctttgc cgcccgcgcc catatagacg gctggcagcg cgacgttgtc
     6661 ccttgcggtc agcgagctta agaggttgta gcgttggaag atgaaaccga agcgttcgcg
     6721 gcgcaatgcc gccagctcgt caggctgcat tttggcagtt tcgatgtcgt cgattcggta
     6781 cgaaccggaa ccggcggtat ccaaacagcc gagtatgttc atcagcgtgg acttgcccga
     6841 accggactgc ccgatgatgg cgacaaaatc gcccttctct atcgacaggc tgatgtcttt
     6901 caaaatatgg acgcggttct cgccgctgcc gaaatagcgg ttgatgtttt tacattcgat
     6961 caagctcata atattccgta aatcaataac aaatgccgtc tgaaaccgtt tccgtgttca
     7021 gacggcatat tcgtttatcg gcgcggcggg ccgcctaggg cgcgttcgcc gctttcctgc
     7081 tgctcggcgg cggttatttc ggagatgacc actttgtccc cctctttcaa cccgcttttt
     7141 acttcggtat tcatactgtc tctcataccg gtccggattt cgcgttccgc cgccttgccg
     7201 tccgcaccca acacgcgcac aaacgccttg ccgccgcgat ttttcacggt cagcgacgga
     7261 ataatcagca catttttcac gccgtcgatt tcaaccgtat tctgcgtcgt catccccgtg
     7321 gcgagtttgc cgtccggatt cggcacaaac gaacgggcat aatagtagac cgcattggaa
     7381 gccgtatccg tactgctgtt gtagccgccc gacgacatcg tggtcagccc ggggtcgacg
     7441 ctgtcgagct tcgccttaat cggcgtatcc ggttcggaca aaatcgtaaa cgaaatatcc
     7501 tgccccgcct tcaccttggt aatatcgccc tcggcaatct gcattttgtt caacatcata
     7561 tccagattcg ccaattggac aatcgtcggc gtagactgcg ccgcgttcac agtctgcccc
     7621 tcttccacga gaatcgccac caccgtgccg tccatcgttg cggtaatgcg cgtgtagccc
     7681 aattccgact cggcggtatt gatggaaatt ttgctctgtc tgattaaagc cttcagctcg
     7741 gcaacattgg ctttggcggc ggcaaacgca tcctgcgcgc tttccaaatc ctctttggaa
     7801 gtcgcgtttt ccttccataa cgccgcctga cgcttatatt tcttctccgc actgcccaat
     7861 gcaatctgtg ccgacaccag cttcgcctga tacgtttcca acttggattt ttccgtattg
     7921 agcgtattgg tctgcgaggt cgaattgatt tccgcaatca aatcgccctt tttaacctgt
     7981 tgcccgagtt tgacataaag tttcttaatc tgccccgatg cctgcgcgcc gaccgatacc
     8041 aggttggacg gcgaaatctc ccctgttgca gaaaccgtcc ggctgatgtc gccgcgcctg
     8101 accgtttccg taatataagc agcctgcggc tcgggcttca gataagacca tccgccccaa
     8161 accgctgccg ccgcgaccgc cgcaacagcc gcccatttca tcatttttgc catgttttca
     8221 attctctgta tgcgtcaatc ccataaatcg gaagcggatt ctaccgcaag tccctgcccc
     8281 atccaaggcg gcaaactgcc gacaataaaa ataaccattt gttttaaatg gcttatacaa
     8341 tctgctttcc cgaaaatttc aatccgttta catattccgc catttccgat acggcgttat
     8401 aatgccgtcg accgacaaac aaatggaaac acattgctat gaaaaacgtt caaaaaggct
     8461 ttacgctgct cgagctgatg attgccgtcg ccatcctcgg catcctgacg cttatcacct
     8521 acccttccta taaaacctac atccggcgcg tccgcctgtc ggaagtcagg acgaccctgc
     8581 tgcacaacgc gcagaccatg gaacgttact accgccaaaa agggacgttt gcaacctacg
     8641 acaaaaacaa actgaaacaa aacgaatatt tcaagattac cttaagcgaa gtcagccccg
     8701 accacttcac ccttcaggca gcccccgatc cgatgaccaa cgacggcgaa acctgcgtcg
     8761 ttacgctcaa cgacggcggt accattgccg cttccggtac aaaccaatcc tgccccggct
     8821 tcgactaatc ctccgccgaa gcccgcaaac ccgtaaagat gccgtccgga cacagcttca
     8881 gacggcattt ttacgggttt gccgtccggt ttttcaaact tgaaacctgc cgcccctcat
     8941 ccggcaaccg ccccgatgcc gtgcctttcc aagatgttat aatataacat ataatctata
     9001 tttttcaaca acataccact tgacctgtga gatgcggaag cgtatgctta cccttatatc
     9061 ggacaacatt ccgtttacag gtttttttgc cgtaactaag gagcgcaatc atgaaaatgc
     9121 aggcagttgt tgtgaataaa aatgtagcgg gcgatgtgga aatagtcgaa cgcgaggttc
     9181 gcccgttgga atacggcgag gcgttggtcg aagtcgaata ttgcggcgtg tgccacaccg
     9241 acctgcacgt tgcggcaggc gactacggcg aaaaaccggg ccgcgtgctg ggacacgagg
     9301 gcatcggttt ggttaaagaa gttgccgacg gtgtgaaaaa tctgaaagtc ggcgaccgcg
     9361 tcagcatcgc ctggctgttc caaagctgcg gctcttgcga atactgcaat accggccgcg
     9421 aaaccctgtg ccgttccgta ttgaacgcgg gctacaccgc cgacggcggt atggcgaccc
     9481 actgcatcgt caatgcggat tatgcggtca aagtccctga aggtttggat cctgcgcaag
     9541 cttccagcat tacttgtgcc ggcgtgacca cttataaagc cattaaagtt tccagtgttc
     9601 gtccgggaca gtggattgcc atctacggcg cgggcggttt gggcaacttg ggggtccaat
     9661 acgcgaaaaa agtattcggc gcgcacgttg tcgccatcga catcaacgac gacaaactgg
     9721 cgtttgccaa agaaaccggc gcggatttgg ttgtcaacgc cgccaaagag gacgctgcca
     9781 aagtgattca ggaaaaaaca ggcggcgcac acgctgcggt cgtaaccgcc gtatctgctg
     9841 ccgcattcaa ctctgccgtg aattgcgtcc gcgcgggcgg acgtgtggtt gccatcggac
     9901 tgccgccgga atcgatggat ttgtccatcc cgcgtttggt tttggacggc atcgaagtgg
     9961 tcggctcttt ggtcggcacg cgcaaagatt tggaagaagc cttccaattc ggcgcggaag
    10021 gtttggttgt gccgaaagtc caactgcgtg ctttggatga agcacccgcc attttccaag
    10081 aaatgcgcga aggcaaaatc accggtcgta tggtgatcga tatgaaaaaa gaatgcggct
    10141 gcggccatca ccactgattt gacgtggcag tacacatcga aatgccgtct gaacgctgtt
    10201 cagacggcat tttttatgga tttggatttg attttaatct gttctgtttg aaataccgtc
    10261 tgaaaaccct tatcgcaaca cgtcattaaa aacggcaaga ttcagccgtt ctgcaacccg
    10321 ttcaaacggc ttccgccatt tccaacgcct gcttgatgtc cacggcgacg acgcgcgata
    10381 cgcctttttc ctgcatggtt acaccgacca gctgttccgc catttccatg gtcaggcggt
    10441 tgtgtgagat gtagaggaat tgggtttgcg ccgacatttc tttgaccaaa ttgcagaagc
    10501 gcgaggtgtt ggcatcgtcc agcggggcat cgacttcgtc caaaaggcag aacggagcag
    10561 ggttgaggct gaacagggcg aacacgaggc tcatggcggt gagggctttt tcgccgccgg
    10621 aaaggaggtg gatggtgctg tttttcttgc cgggcgggcg cgccatgatg gacacgccgg
    10681 cggtcaggag gtcgtcgcct atcattttga gggtagcttc gccaccgccg aacagggtcg
    10741 ggaagaaagt ttggactttg ccgttgacgg catcgaaggt ttctttgaaa cgcgctttgg
    10801 ttttgtcgtc gatttgggcg atggcttctt ccaaaagggt gatggctgcc tgcacgtctt
    10861 cgctttggct gcggtagtag ccgtcgcgtt cgcgcgcttc ttcgagttct tgcagggcgg
    10921 cgaggttgac cgcgccgagt gcttcgattt gttgcgaaag gctgccgatg ctgctgttca
    10981 atactttcgg cgattctttc gccaacgctt cgagcgcgtc caaatcggcg gcgcgttcgg
    11041 tcaggttttg atggtagcgt ttggcgttga tcagggcttc ctgctgctgc aacaaggcgg
    11101 tttgggtggc ggcctgaagc tgcggcagct tggtttgcag ggtttgcact ttagcgtatt
    11161 gctccctgcc ctgttcctga atctgcgcga gtttctcttg cacaacaata tattcttcgt
    11221 ccaaggtctg tacggcttcg cttaattctt caagcttgat gtgctgctcg tcgttttgga
    11281 actcggtttc ataggcgagg gcaagctctt gctggcgttc ctgccagtct agggtttgct
    11341 gttcgagctg ggcaatttgc tgccggtagt tttgtttttg ctggttgagc ttgtgaacgg
    11401 cgacttcggc cagcccgtat tggcggttgg cttccaacag ggcaagctgc gcctgtttca
    11461 gacggccttg ctgctcttgg cggctgtgcg cggtggtttg ctgctggtgt tcgagttcgg
    11521 cggcggcttc ctgcaaggta acgatgtcgt ctgaaagccc gtcggacgtg tgttgcaaca
    11581 cggtctgctc ttccgccaac tgcgccagtt cgcgctcgat gtgttcgcgg cggatttgcc
    11641 cttggttggt gcgcgccaga agttcggcgg cgcgttgctg cgcctgactg tactggcgcg
    11701 tatgctgctg ttgttgctgc atcaggtttt tgtgctgcac ttcggacgag cgcacggcgg
    11761 cttccgcctg tttgaacgcg gcttcggcgg cggagagttc gggggctagg ttgtccagtt
    11821 cggatgcgat gccgtcgagg cgcgcttttt gggcaatcag gctttcctgc gcgggcttgg
    11881 catagagcag gacgctgact ttatcgacct gatggccttc gggcgtaagc cagatttgat
    11941 gcgcgcccaa atcgctttga tgagcgaggg cgtaactcaa atcgggcgca cacaatacgc
    12001 cgtcgagcca gtggtgcaat gccgtctgaa acggcggctg cgcttggatt tggttcagca
    12061 atgcctgtac gggcagcgat tttttgatgc caccggagag gccgtctgaa agccacgccg
    12121 cctgcccctg cggcaaaggt tcggggggaa cgaaaccttg cggcacggcg cgggcgtgca
    12181 ggcgttcggc aaggatgacg gacaaggcgt gctgccactc ggcgggcgcg gtgatgtgtt
    12241 gccacagttg cggcgcggcg gcgtggtcgc ttgcctgcca gaagtcggcg gcttcctgct
    12301 gttgcgacag gatttgcgac aacgcctgct gctgcgcctg caaggtgatg tgttgctgct
    12361 tcaggctttg gaagcggttt gatgccgtct gaaacgcctc gcgggcggcg tgtagagctt
    12421 cttcggcggc gatgatttgt tcttcgtaat gctcttgccg gttttgcaac aaggcggcgg
    12481 cttcctgcgc ggcggcggtt tcggcttcgt cgggcaggtt cagggcttgg ttttcctgtt
    12541 tcagacgacc tttgcgctct tcgtgcttgg caacggtttg ttcggcatgg gcaagctgct
    12601 gctgcttcaa cgccagttcg cggcggatgc ggtttgcctc gtcctgccgg gtttggaagg
    12661 cggcgttgag cgtggcttgg gcttcttcca attcgggcag acgctcttcg tgttcggcaa
    12721 cctgcatcgc ccattccgcc aactcggttt gcttttcttc tgcctgcaac tcgttttcct
    12781 caagctgcac gcggatttgc tgctgttctt gatggatgcg ctgcatctgc gcctgcgccg
    12841 cctgcttgtc gcgttcgatg cgttggtgca ggttttgctg atggcggatt tgttcttcca
    12901 aacgggcaat ctgctcgcgc aacacgccgc gtttgttgct caattcgtgt accgcctgct
    12961 gctgcgactg ttcggcggtc tgcaaggcgt gtacttcgtc gtttaacgcc tgaacctgcg
    13021 cggcggtttc gtcctgctgc gcctgcaaag actgatgctg cgcggtcgcc ttgtcggcgg
    13081 cggcaagcga ttgctgccat tgggcgtaat cgagcaaatc ctgctgttgg ttcaactgcg
    13141 cggtcaggga tttgtagcgt tcggcggttt ccgcctgttt ttccagcttt tccacctgac
    13201 gcgccaactc gttctgcaaa tcacccaaac gctgcaaatg ctcgcgcgtg tctttcagac
    13261 ggccttccgt ctccttgcgg cgttccttat atttggacac gcccgccgcc tcctcgatat
    13321 aggcgcgcaa ctcctccggc cgcgcttcga tgatgcgcga aatcatcccc tgctcgataa
    13381 cggcataacc gcgcgcgccc acgccagtac ccaaaaacaa atcggtaatg tcgcggcggc
    13441 gcacggtctg attattgatg aaataagtcg attcgccctg acgcgtcaac tgccgcttga
    13501 tgctcacctc ggcatactgc ccccacgccc cctgcaaact gtggtcgctg ttgtcaaaca
    13561 ccagctccac cgaagccctc ggcgcaggac ggcgcgtcgc cgcaccgtta aaaatcacgt
    13621 cctgcatact ctcgccgcga agctgcttcg ccgaagcctc acccaacacc cagcgcaccg
    13681 cgtcaatcac attcgacttg ccgcagccgt tcggcccgat aaccgcgaca agctgccccg
    13741 gaacatgaat cgtggtcggg tcggtaaaag atttgaagcc ggagagtttg atgtgggtca
    13801 ggcgcataat ggtcggaaaa aataaaaaag aagcgtattt taacggaaat cccgccgcac
    13861 caccccatat ctttctggca aaaccttacc gcatcctacg cccgcgatgc cgtctgaagc
    13921 cttcagacgg catttttaac gcgcccgaac cccggttccg ccaatgcccg atgcttggcg
    13981 cggcatttgc cgtattgccc cgccgccggg cgcgttataa tccgccacga catccgcccg
    14041 atttggaaac ctgcctatga aacccgccta tttcatttcc gacctgcatt tgagcgaaaa
    14101 gcagcccgaa ctgaccgcgc tgctgctgcg ttttttacgt tcttccgccg ccgggcaggc
    14161 gcgggcgatt tatattttgg gcgatttgtt tgatttttgg gtgggcgatg acgaagtttc
    14221 cgagttgaat acttcggttg cgcgggaaat caggaaattg tccgacaaag gcgttgccgt
    14281 gttcttcgtc aggggcaacc gcgacttcct gatcggtcag gatttttgcc ggcaggcggg
    14341 tatgacgctg ctgccggatt actcggtttt ggacttgttc ggctgcaaaa ccctcatctg
    14401 ccacggagac acattatgca ccgacgacaa ggcataccaa cgtttccgca aaatcgtgca
    14461 tcggaagcgg ctgcaaaaac tgttcctgat gctgccccta aagtggcgca cgcgccttgc
    14521 cgccaaaatc aggcgtgtca gcaaaatgga aaaacaggtc aagcccgccg atattatgga
    14581 tgtcaatgcc gcctttaccg agcggcaggt tcgcgccttt aacgcggaaa ggctgataca
    14641 cgggcatacc caccgcgagc atatccatca cgaaaacggc tttacccgca tcgttttggg
    14701 cgactggcat aacgactatg cttcaatcct ccgcgtagac ggggacggcg cggtattcgt
    14761 gccgctggaa aaatgctgaa aatgccgtct caagcctttc gggcggcttt ttttacacgc
    14821 ccctttccca cactccctcc gccgcatcat caccatcctt aacccgcatt aaggggcgtt
    14881 atcgataaaa tgcttgaccg attacataag attacgtaaa gtgtgcaaac gcataacaat
    14941 tggtcttacc aatttatctt ttaaccaaaa tatcgggtgt acgggatttg aacgtctttc
    15001 caaacctcga cgcgccttat gatgcaaatg cctaaaaagg attcgttatg gcacttttcc
    15061 tcagcatatt ccccatcgtc ctgctgattt ggctgatggt gaaaaaaaac agtatgccct
    15121 cctacgtcgc gctgccgatt accgcagtgc tgatttacgc catcaaactt ttctacttcg
    15181 acgatgcagg aatgctgctc aacgccaccg ccgcttccgg cctcgtcaaa acgctcacgc
    15241 cgattaccgt gattttcggc gcgattatgt tcaaccgtat gatggaaacc acgggctgca
    15301 tcgatgtcat ccgcaaatgg ctggcgacca tcagccccaa ccccgtagcg caactgatga
    15361 ttatcggctg ggcttttgcc tttatgattg aaggcgcatc cggcttcggc acgcctgccg
    15421 cgattgccgc gccgattctg atgagcttgg gcttcaaccc gttgaaagtg gcgattttca
    15481 ctttggtgat gaactccgtc cccgtctctt tcggcgcggt cggtacgccg acctggttcg
    15541 gtttcgcacc gctgaacctg agtgccgaag acatcctcgc catcggcagg cagaccggcg
    15601 taatgcactt cttcgcaggt ttcgtcatcc ccgtcatcgg cttgggcttc atcgtacctt
    15661 ggtctgaaat ccgcaaaaac ttgggcttcg tcgccattgc cgtcttctcc tgcaccattc
    15721 cttatgtcgc attggcgatg gtcaacgaag aattcccgtc gctcgtcgcc ggcgcgatcg
    15781 gcctgatggt gtccgtattc gccgccaacc aaggctgggg cttgagcaaa gaccacgcca
    15841 aagacccgaa tgccgaaaaa gtgccgttcg cccaagtcgc caaagcactc gcccctttgg
    15901 gtatgctgat cggcatgctg gtggttacgc gcatcaaaca gctcggcatc aaaggcattt
    15961 tgaccagcaa agaagaatgg ttcagcttcc aactgccgtt tgatttgtcc aaaatcaccg
    16021 tcagcgactc cctgacgatt accttcggca atattttcgg acaagatgtc agcgcgtctt
    16081 accaaacgct gtacgtcccg gcttggattc cgtttgtgct gaccgtttgg atttgcatcc
    16141 tgctgtataa aaccaaattc aaagatgcct ggacgattta ttccgtaact ttcaatcaaa
    16201 ccaaaaaacc gctgcttgcc ctgatgggcg cgctgattat ggttcagctg atgctggtcg
    16261 gcggcgacaa ttcgatggtg aaaatcatcg gtaaggaatt tgccgcaatg gcgggcgaac
    16321 actgggttta tttctcgccg tatctgggcg cgatcggcgc gttcttctcc ggttccaaca
    16381 ccgtgtccaa cctgaccttc ggcccgattc agcagcaaat cgccttggat accggcctat
    16441 ccgttaccct gattctggcg ttgcagtccg tcggcggcgc gatgggcaat atggtgtgcc
    16501 tcaacaacat catcgccgta tgtaccgtat tggatgtgaa aaattccgaa ggtgcgatta
    16561 tcaagaaaac cgttatcccg atggcgattt acggcgtgat tgccgtcgtc gcggcaatga
    16621 ttttcttcct ctaggcgaaa cgccaccatc catagcggca gcacgcgggg aatggaaacc
    16681 gccgttcccc gtctgggcgg cgcgtcgggc agaatcccgg tgccggaaca agcccgcccc
    16741 gccaaacaaa tgccgtctga aaccgaaaaa ggcttcagac ggcatttttc cgccgtttgc
    16801 gttcaggcgt aatgatgatg cggcatcttg ccgtcaaggc ggtagcgcgt gccgcaatac
    16861 gggcaggcaa cgctgcccga ttctccttcg cacaaaggca aaaacactct cggatgtccg
    16921 ttccactgct cgttgccggg tcccgagcaa tacagcggca gattttccgg caacacggaa
    16981 atttcctgca gattcagatt gtccatttga ttttcctttg cgtggttggc gtgcgcccat
    17041 tttacgccat cggcaggcta aaggatattt tcggcgcaaa gccgcaatcc gctataatcc
    17101 ccacttttca gacggcatac catgactgcg cttacccttc cggaagacat ccgccaacaa
    17161 gagccatccg ccctgctcta taccctcgtt tccgcctacc tcgaacacac cgcccaaacc
    17221 ggcgacgaat ccctctcctg cctgtccgac gaccagcaca cgctgaccgc attctgctac
    17281 ctcgacagcc aagtcgaaga aggcggcttc gtgcaactca tcgcctccgg ctacggcgaa
    17341 tatattttcc gcaacccgct tgccgacagc ctgcgccgct ggaaaatcaa agccgtgccg
    17401 aaagtcttgg acaaagccaa agccctctac gaacaacacg gcaaaaccat cgaaacgctc
    17461 gccgacggag gcgcagacat cgaatccctg cgcaaacagt tccccgactt tgaagaatgg
    17521 gacggcgcat actacgaagc cgccgaacaa gacctgcccc tgcttgcaga acacatacag
    17581 tcaaactggg aaaccttcgc ccatatcggg caggcgtgat tgcgtctgtt tccagctcgt
    17641 gtaaaacagc gtaaaatcgg caaacccgaa tgaccttccg tccccatcaa ggagcaagct
    17701 atgttcttca agcacatcga agccgccccc gccgatccga ttctcggttt gggcgaagcg
    17761 ttcaaagccg aaacccgccc cgaaaaagtc aacctcggca tcggcgttta taaagacgca
    17821 tccggcgcga cacccctcgt caaagccgtc aaagaagccg aaaaacgcct gttggaaagc
    17881 gaaaccacca aaaactacct caccatcgac ggcgttgccg actacaacgc gcaaacccaa
    17941 atcctgctgt tcggcaaaga ccacgaaatc atcgccagcc gtcgcgccaa aacagcgcaa
    18001 agcctcggcg gtacgggcgc attgcgtatt gcggccgagt ttgccaaacg ccagttgaac
    18061 gcgcaaacca tctggatttc caatccgact tggcccaacc acaacgccat cgccaaagcg
    18121 gtcggtatcc aagacaaacc ttatcgttac tatgatgccg ccaaacacgg tttggattgg
    18181 gacggcatga ttgaggactt gagccaagcg caaaaaggcg acatcgtcct gctgcacggc
    18241 tgctgccaca atcctaccgg catcgaccct acgcccgaac aatgggaaac tttggcaaaa
    18301 ctttctgccg aaaaaggctg gttgccgctg tttgactttg cctaccaagg cttcggcaat
    18361 ggtttggaag aagatgccta cggcttgcgc gtgttcttga aacacaatac agaattgctg
    18421 attgccagct cttattccaa aaacttcggt atgtacaacg agcgcgtcgg cgcgttcact
    18481 ttggtggccg aagatgaaga aacagcagcc cgcgcccaca gccaagtcaa aaccatcatc
    18541 cgtaccttgt attccaaccc ggcttcacac ggtgcgaaca ccattgcgct ggtgttgaaa
    18601 aatgatgatt tgaaagcaca atggattgcc gaactcgatg aaatgcgcgg ccgcatcaaa
    18661 gccatgcgcc aaaaatttgt cgagttgctc aaagccaaag gtgcaagcca aaactttgat
    18721 ttcattatcg aacaaaacgg tatgttctct ttcagcggct tgactcccga acaagtcgac
    18781 cgcctgaaaa acgagtttgc catttatgcc gtccgctccg gccgcatcaa cgtcgccggc
    18841 attaccgacg acaacatcga ttatctgtgc gaaagcatcg tgaaagtact gtaaaaagca
    18901 cagccatatg aaaatgccgt ctgaacctta accagttcag acggcatctg ttttttgtta
    18961 aaaaacaata aaccataaca atttcaaaca agctaaaaac atctataaaa tcaacccaat
    19021 atgatgaact caaataaaaa atacgaatat catcattctc aaattcaaaa tttgaacgcc
    19081 acagtattat ccgaagccac aaaaaccttt ccgccgtcat tcccgcgcag gcgggaatcc
    19141 gggaacccaa tgctgcaaga attgattaga aacaactgaa accgagcaaa ctggattccc
    19201 gcctgtgcgg gaatgacgaa ataaggcgag taaaactgat gaaacaaatt gattaaaaac
    19261 aataacttat attattttat ttaaattttc gataaaatca attttccgta ttcaatactg
    19321 ctccaatcaa ttcccgcgca ccgtcgtccg ccaatttctt agcgacagcg cgtccgagcg
    19381 cgtcggcata ttcggcaggg gcttgcgcgt ccgcctgcaa aatgaccgaa ccgtcggggt
    19441 gtccgaccag tccgcgcaag gtcagcaagc cgttttcttc cgtgcaatat gcggccaaag
    19501 gcacttggca gcttccgccc aaagcgcgcg ccaaagcgcg ttcggccgta acgcaggcgt
    19561 gtgtggtatc gtggtttagc ggttttaaaa cctcatacaa atcttcgcgg tgcgcggcaa
    19621 tttcgatacc caatgcgcct tgtccggcgg caggcaggct gtcggattcc gacaaaatca
    19681 tgcggatgcg tccgtccaat tccagacgct gcaaaccggc ggcagccaag ataattgcgt
    19741 cgtattcgcc gttatcgagt ttagacaaac gggtttgcac attgccgcgc aaaggtttga
    19801 taaccaaatg cggatagcgc gcacgcaact gggcttcgcg gcgcaggctg gatgtgccta
    19861 cgacggcgcc tttgggcatt tcttccaaac gcgcgtattg gttggacaca aacgcgtcaa
    19921 acggattggc gcgttcgccg atggctgcaa gcgcgaaacc ttcaggcaaa tccatcggca
    19981 catctttaat cgaatgcacg gccaagtcag cgcgcccgtc atataaagcc tgttccaact
    20041 ctttgacaaa caagccctta ccgccgactt ttgacaaagt tttgtccaaa atctggtcgc
    20101 cgcgcgtggt catgcccaaa atctcgactt cgcaatcggg atacagggct ttcagacggc
    20161 cttggatatg ctttgcctgc cacatggcaa gcaggctttc gcgactggcg ataacgagtt
    20221 ttttcgggtt cataatggtt gcctgaaaga tggaaaacgg catgaagtct atcatatttt
    20281 gggcggtctt tcagacggca tgagggtgaa tatgttatat ttacgcctaa aaaccaaatt
    20341 ttccggacaa catgaaacgc gccaaacaat atcctttttt atccttacag cgacaacgtt
    20401 ttcatctgaa ctttgaaaac gcttcttcca ccgccaacct tccgagcgaa cgcgatttct
    20461 accgctgggc gtggtctgcc ttgaaaaatg aataccgccg cgccgacatc agcctgattc
    20521 ttctggacga agaagaagcc cgcgcttaca accgcgacta ccgcggcaaa gattacgcca
    20581 ccaatgtatt gagtttcgcg ctcaacgaag gcgaaatcct gccctgccaa gtttcggaaa
    20641 aactgtacgg cgatttgatt atctgcccgc aagtggtttt gaaagaagcc gccgaacagg
    20701 gcaaaacgcc cgaacggcat tttgcccacc tgaccataca cggcgttttg cacctgatgg
    20761 gctacgacca catcgaagac gatgaagccg aaataatgga agccgaagaa atccgcctga
    20821 tgctggcggc aggcttcccc aacccctacc aagaggacga atattaaaat ggacggtacg
    20881 cagtcgaaac ccaagttttt cgaacgcctg atctcccgac ttgccggcga gcccgactcc
    20941 gccgaagacg tattgaccct gctgcgccaa gcgcacgagc aggaagtttt tgatgcggat
    21001 acgcttttaa ggttggaaaa agtcctcgat ttttccgatt tggaagtgcg cgatgccatg
    21061 attacgcgca gccgtatgaa cgttttaaaa gaaaacgaca gcatcgagcg catcaccgcc
    21121 tacgtcatcg ataccgccca ttcgcgcttc cccgtcatcg gcgaagacaa agacgaagtt
    21181 ttgggcattt tgcacgccaa agacctgctc aaatatatgt tcaaccccga gcagttccac
    21241 ctgaaatccg tcttgcgccc tgccgttttc gttcccgaag gcaaatcttt gaccgccctt
    21301 ttaaaagagt tccgcgaaca gcgcaaccac atggcgattg ttatcgacga atacggcggc
    21361 acgtcgggtt tggtcacctt tgaagacatc atcgagcaaa tcgtcggcga aatcgaagat
    21421 gagtttgacg aagacgacag tgccgataat atccacgccg tttcttccga acgctggcgc
    21481 atccacgcgg ctaccgaaat cgaagacatc aataccttct tcggcacaga atacagcagc
    21541 gaagaagccg ataccatcgg cggcctggtc attcaagagt tagggcatct gcccgtacgc
    21601 ggcgaaaaag tccttatcgg cggtttgcag ttcaccgtcg cccgcgccga caaccgccgc
    21661 ctgcacacgc tgatggcgac ccgcgtgaag taagcagagc ctgcccgcac cgccgtttct
    21721 gcacagttta ggatgacggt acggacgttt tctgtttcaa tccgccccat ccgccaaaca
    21781 taaaaatgcc gtctgaaacc ggaaaacagg ttcagacggc atttcgctta tccgcttatt
    21841 ttttgctcaa agtcagcccc gtccagccga acagaagcgt cagtgccaaa ctcaaatagc
    21901 agaaaaaggc atacggcaga tattcccaca ccggcacacc cagcgcgtgg ctgataaata
    21961 cgccgcacac gctccacgga acaagcgggt tgatgaccgt acccgcgtct tcgagcgtac
    22021 gcgagaggtt gcgggaatgt agtccgagcc tgtcgtaaac gggtttgaac gtttctcccg
    22081 aaagcaggat gctcaaatat tgttcgccaa tcaggaaatt gaccccgacc gaagtcatgg
    22141 caacactgaa cgtcgcgcgt ccggcattcg tcaaaaagct gcggacggca tccagcaggg
    22201 aaggaatcgc gcccagcgca aacagcagtc cacccaaact catcccgaga atcacgatgg
    22261 tttgcgtgaa aaacatactt tccaaaccgc cgcgcgaaat cagtttggca acatctttaa
    22321 acgcttcgcc ttcgagtttg taaccgccgt aaaaccacgc gccgagctga cgcagatcgg
    22381 gcgtgctgtg cagatacgtt acggcaacgg cggcaatgac ggtaaagagc atggcgacca
    22441 cggcattgac gcgcatcaat gccaaaacga ccaacagtgc aaacggaatc agcgaatagc
    22501 cgtgcaccaa tcccgtggct tcaagctggc tgcggaagga ttcgacgctg ttcaaatcct
    22561 gcgcggcgac attcggcaaa agccaaagca tcagtgccgc actaatgagc cacgcgggga
    22621 tggtggtgta catcatattt ttgatgtgct caaacaagtc gatgccgacg atggacgcgg
    22681 aaatgcccgt ggtgtcggaa agcggggaca ttttgtcgcc aaaaaatgcg cccgaaacaa
    22741 tcgcgcccgc cgtcatcgcc atatcggcat gaaacgccgc cgccatcccc ataaaggcaa
    22801 cgccgacggt ggcgcaggtg gtcaggctgc tgccgatgga cacgccgatg acggaacaca
    22861 gcgcgaaagc ggaaaaataa aaataagtcg gggaaatcag cccgaaaccg taatacatca
    22921 gcgtcggaat cgcgccgctc atcatcagcg cgctgaccat cagcccgatg aagaaaaaca
    22981 ggtaaatcgc gcccataccc tgattcaacg cgcctatcat gccctgctgc atatcgttgt
    23041 atttcaaacc gcgcaccaag ccgtacaaaa tcagcacgac gatggcggca ataatggaca
    23101 tatgcggcag ccactccaat gaaatgatgg tatagcccat cgcggcaatc agagcgacga
    23161 cgacggcaag tgcctcaccg cgcggcatat cgagtaagga tttgaaagca aacatgattc
    23221 tttgcccgat ttgatataaa gttaacggat tctaacacac cataccgaat ccgcccaacg
    23281 ttttcagacg gcattttagc gttttacaaa agccggacag aaacaaaacg gcaattttct
    23341 caatttaaat taattttttt gaaatataat tttttatttt tttgtttgtt ggcatataag
    23401 acttttatgg attgaagtat taaaacctcg gcacacatgc cgcaaacacc ggtacgcaaa
    23461 aatgccgtct gaaagatgtt cagacggcat ttctcaagaa aaagattatt tgtccgccct
    23521 atatccgtac accgcaaaaa acacgatata cagataacag atgacggaaa cgacaaacga
    23581 agacatcagg gtgtaagtat ctgccaccca gccctgcacg acaggaacga ccgcaccgcc
    23641 gacaatcgcg gtacacagta caccggaagc cgcgttggta aattttccca atcctttggt
    23701 tgccaaagag aaaatcgtcg ggaacataat cgagttgaaa aaaccgatgg caagcagcga
    23761 ccacatcgcc acatccgcat tgccgctgcc cgtcgccatc gcgacggcaa gcagtacgac
    23821 cgcagccgat gcgttaaacg ccaaataacg gttgggcgcg aatttcgcca tcaccgccga
    23881 accgaggaaa cgtccgacca tcgcgccgcc ccaatagaac gacaggtaat gcgcggcaga
    23941 agcatgatcc agccctttca gataacccaa tacgttgacc atcaacgaac cgatagacac
    24001 ctccgcgccg acatagcaga aaatgcctgc cgtaccgaac acgagatgtt tgtattgcca
    24061 tacgctggtt ttgccgtcgt ggttgtgcgc gctttcctcg gcggcaattt tgcgcgcgtc
    24121 gggcagccgg atcattttca cgaaaacggc aaggataatc agcagccccg ccagtcccaa
    24181 atagggaatc tgtacggaag aaatctgttc cgccttgctg acggtttggg ttgcgtccgc
    24241 cagaatcagg aacgcgccga tttgcggcgc aatggtcgta cccaaagcgt taaacgcctg
    24301 aaccagcgtc agtgttgccg attccttgcc gggtttcgcc agcagggtaa cataaggatt
    24361 accggcgacc tgaagcagcg ttacgccgga agccaaaata aacaacgcgc ccaaaaatac
    24421 cgcgtaagaa tggctgcccg cagcaggata aaacagcagg catccgaccg ccgtcagcag
    24481 aaagccgccg ataacgccgt ttttgtagcc gactttgccg acaaaagccc ccatcgggat
    24541 ggacatcacc gcataggcgg taaagaaaca gaattggatc agcatcgcct gaacgtaaga
    24601 caggtcgaaa atttctttca aatgagggat aaggatgtcg ttcatgcagg taataaaacc
    24661 catcatgaag aacagcgtgg tcaagacgac caatggggac gtatgattgt tttgtgattg
    24721 cgcagacatg atttgccttt cgtggtgtcg gaatattacc cgtttcaaga aaatagaaat
    24781 tgacatttct taacggcaat gcaaatcata ctgatatttg aacattttgc aaactttatc
    24841 ggatgcaggc aatgcgccga tctgaaattc aagcagcaga aattaatatt ttacagttgc
    24901 ttgcatagat tttaatagat tttaatagat tttaaaaaat tccggcggac ttgcaaaaac
    24961 cgttcccaat aaaatgaaaa tccgccgccg tatccggaaa attcggaaat gccgcatcgt
    25021 ggcatcaaaa ccggagcgga aagaatgtcg tatgcccgac aaaaaagggg catacggctg
    25081 tgccgtattt tttgcaccgt cctccgccct tcggcgggg
//
LOCUS       DAXHRI010000013        25036 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXHRI010000013 DAXHRI010000000
VERSION     DAXHRI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25036
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.013"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            232..855
                     /gene="lolA"
                     /locus_tag="ACFM75_000382"
     CDS             232..855
                     /gene="lolA"
                     /locus_tag="ACFM75_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC6254982.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1128..2267
                     /locus_tag="ACFM75_000383"
     CDS             1128..2267
                     /locus_tag="ACFM75_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6254983.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2399..3442
                     /locus_tag="ACFM75_000384"
                     /pseudo
     CDS             2399..3442
                     /locus_tag="ACFM75_000384"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            3483..4013
                     /locus_tag="ACFM75_000385"
     CDS             3483..4013
                     /locus_tag="ACFM75_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC6254984.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYIGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(4030..4161)
                     /locus_tag="ACFM75_000386"
     CDS             complement(4030..4161)
                     /locus_tag="ACFM75_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC6254985.1"
                     /translation="MPSEPEKRLSDGIFACNSNAAGQNQGASDTVIGHPVRMNLGLT"
     gene            complement(4386..5981)
                     /locus_tag="ACFM75_000387"
     CDS             complement(4386..5981)
                     /locus_tag="ACFM75_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC6254986.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(6088..6483)
                     /gene="hisI"
                     /locus_tag="ACFM75_000388"
     CDS             complement(6088..6483)
                     /gene="hisI"
                     /locus_tag="ACFM75_000388"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC6254987.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(6514..7281)
                     /gene="hisF"
                     /locus_tag="ACFM75_000389"
     CDS             complement(6514..7281)
                     /gene="hisF"
                     /locus_tag="ACFM75_000389"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC6254988.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGNPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAANPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(7294..8031)
                     /gene="hisA"
                     /locus_tag="ACFM75_000390"
     CDS             complement(7294..8031)
                     /gene="hisA"
                     /locus_tag="ACFM75_000390"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC6254989.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(8064..8702)
                     /gene="hisH"
                     /locus_tag="ACFM75_000391"
     CDS             complement(8064..8702)
                     /gene="hisH"
                     /locus_tag="ACFM75_000391"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC6254990.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALKRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLLDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQDTRFYFVHSYY
                     FDPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(8833..10335)
                     /gene="pta"
                     /locus_tag="ACFM75_000392"
     CDS             complement(8833..10335)
                     /gene="pta"
                     /locus_tag="ACFM75_000392"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC6254991.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGFAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRNANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(10520..11578)
                     /gene="fbpC"
                     /locus_tag="ACFM75_000393"
     CDS             complement(10520..11578)
                     /gene="fbpC"
                     /locus_tag="ACFM75_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC6254992.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRCPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(11599..13116)
                     /gene="fbpB"
                     /locus_tag="ACFM75_000394"
     CDS             complement(11599..13116)
                     /gene="fbpB"
                     /locus_tag="ACFM75_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC6254993.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(13184..14179)
                     /gene="fbpA"
                     /locus_tag="ACFM75_000395"
     CDS             complement(13184..14179)
                     /gene="fbpA"
                     /locus_tag="ACFM75_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC6254994.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(14430..14895)
                     /locus_tag="ACFM75_000396"
                     /pseudo
     CDS             complement(14430..14895)
                     /locus_tag="ACFM75_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(14953..16329)
                     /gene="argH"
                     /locus_tag="ACFM75_000397"
     CDS             complement(14953..16329)
                     /gene="argH"
                     /locus_tag="ACFM75_000397"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC6254995.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASISFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(16348..17217)
                     /gene="galU"
                     /locus_tag="ACFM75_000398"
     CDS             complement(16348..17217)
                     /gene="galU"
                     /locus_tag="ACFM75_000398"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6254996.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(17245..17844)
                     /gene="rdgB"
                     /locus_tag="ACFM75_000399"
     CDS             complement(17245..17844)
                     /gene="rdgB"
                     /locus_tag="ACFM75_000399"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC6254997.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDSA
                     NNLKLVGELACKADKSCCYVCVLVFIRHENDPRPIIAEGVWHGQWHDTPQGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(17837..18070)
                     /locus_tag="ACFM75_000400"
     CDS             complement(17837..18070)
                     /locus_tag="ACFM75_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC6254998.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(18178..18711)
                     /locus_tag="ACFM75_000401"
     CDS             complement(18178..18711)
                     /locus_tag="ACFM75_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC6254999.1"
                     /translation="MADFNQILTTGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(18813..19271)
                     /gene="nudB"
                     /locus_tag="ACFM75_000402"
     CDS             complement(18813..19271)
                     /gene="nudB"
                     /locus_tag="ACFM75_000402"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC6255000.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFDMEEAAEKVFSSSNRCAILELGRFLGKR"
     gene            19482..19558
                     /locus_tag="ACFM75_000403"
     tRNA            19482..19558
                     /locus_tag="ACFM75_000403"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19516..19518,aa:Pro,seq:ggg)
     gene            19716..22115
                     /gene="mafB"
                     /locus_tag="ACFM75_000404"
     CDS             19716..22115
                     /gene="mafB"
                     /locus_tag="ACFM75_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC6255001.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKDVKYDTRINTAVPQVNPIDEPVF
                     NPKGSVGSAHSWSITARIQYAKLPRQGRIRYIPPKNYSPSAPLPKGPNNGYSAGNLSK
                     KVLTLVYINGKLKFGPSSLPSSALKAPVPIVGKAEAPPGKAVPVGEARIPSNPKAVKG
                     TTVTLSQEQRLANGELIPKGSVVTWSDNAVKVVRPDGKKRRFNSRDWEQKALPKPQLP
                     KDYRDDINIGGNNPRFNQQRHEKIESHNPNRIEEIRQRNAVSSAERQADALKRMQTSG
                     RTRLPGQPQKGKPGAAREMSSSDNPNQTAEAFARRYLGRKPTLMERVEGEKLYGGKCV
                     GCWKAQMPDGTFVVYRPAGNASMTKGTTASVDINNPTLNNLNINLKGKPTELKFKFPA
                     R"
     gene            22126..22587
                     /locus_tag="ACFM75_000405"
     CDS             22126..22587
                     /locus_tag="ACFM75_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255002.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            22574..22861
                     /locus_tag="ACFM75_000406"
                     /pseudo
     CDS             22574..22861
                     /locus_tag="ACFM75_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
     gene            22888..23307
                     /gene="mafI"
                     /locus_tag="ACFM75_000407"
     CDS             22888..23307
                     /gene="mafI"
                     /locus_tag="ACFM75_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6255003.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            23397..23468
                     /locus_tag="ACFM75_000408"
                     /pseudo
     CDS             23397..23468
                     /locus_tag="ACFM75_000408"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            23465..23706
                     /gene="imm31"
                     /locus_tag="ACFM75_000409"
                     /pseudo
     CDS             23465..23706
                     /gene="imm31"
                     /locus_tag="ACFM75_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
     gene            23784..24029
                     /locus_tag="ACFM75_000410"
     CDS             23784..24029
                     /locus_tag="ACFM75_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6255004.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMGKGK"
     gene            24032..24418
                     /locus_tag="ACFM75_000411"
     CDS             24032..24418
                     /locus_tag="ACFM75_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255005.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFEMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPVSKEKA
                     LKALEKHPEIYQRFQHFFPFVELPPL"
     gene            24519..24908
                     /locus_tag="ACFM75_000412"
     CDS             24519..24908
                     /locus_tag="ACFM75_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255006.1"
                     /translation="MWKIIKEDSDDLEFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADLDEEIDNIYNIIGFVSSYSLSKLRRNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
BASE COUNT         6019 a         5887 c         6982 g         6148 t
ORIGIN      
        1 gtactatttg tactgtctgc ggcttcatcg ccttgtcctg atttttgtta atccactata
       61 acgcggcaca cattaaaggg cagcgtggcg cgcctgcctt ttccgagcgg tcaaaaaaat
      121 cagccctcgg aaaacgctgt ttgtaaaatg caaaccgccc gtaacgccgc ccgtatgatt
      181 gttttgccgc gccgatactt tacgccacac ttattccaac aaggaaaaat aatgatgaaa
      241 ccgcacaacc tgttccaatt tctcgccgtt tgctccctga ccgtcgccgt cgcttccgca
      301 caggcgggcg cggtagacgc gctcaagcaa ttcaacaacg atgccgacgg tatcagcggc
      361 agcttcaccc aaaccgtcca aagcaaaaag aaaacccaaa ccgcgcacgg cacgttcaaa
      421 atcctgcgcc cgggcctctt caaatgggaa tacacttcgc cctacaaaca gactattgtc
      481 ggcgacggtc aaaccgtttg gctctacgat gtcgatttgg cacaagtgac caagtcgtcc
      541 caagaccagg ccataggcgg cagccccgcc gccatcctgt cgaacaaaac cgccctcgaa
      601 agcagctaca cgctgaaaga ggacggttcg tccaacggca tcgattatgt gctggcaacg
      661 cccaaacgca acaacgccgg ctaccaatac atccgcatcg gcttcaaagg cggcaacctt
      721 gccgccatgc agcttaaaga cagcttcggc aaccaaacct ccatcagttt cggcggtttg
      781 aataccaatc cccaactctc gcgcggcgcg ttcaagttta ccccgcccaa aggcgtggac
      841 gtgttgagca actgatgccg tccgccccga tgccgtctga aagccgccga ggcttcagac
      901 ggcattttca cgcaggcgga acaatgtccc gcattaccgc ccgatcgggc atcggaacgg
      961 caaacccgtg aaaattaacg gttgcgcccc ggctgttttt gccgtttaat gcaaaccttg
     1021 ctgcaccaag ggccaagaaa gccgaccggc cgcccccaca gcttccgatg caggcggccc
     1081 ggccgtccct gcaatgtttt ttatttttga acgaaaggtc gaaaaccatg aaaaaaacac
     1141 tggtggcggc ggcaatcctg agcctcgcct tgactgcgtg cggcggcgga agcgataccg
     1201 ccgcccaagc cccctccgcc aagcccgaag ccgaacaatc gggcaaactc aacatctaca
     1261 actggtcgga ttatgtcgat cccgaaaccg tcgccgcctt tgaaaaagaa accggcatca
     1321 agacgcgttc cgattattac gacagcaacg aaacactgga ggcaaaagtc ctgaccggca
     1381 aatccggcta cgacctgacc gcgccgtcca tcgccaacgt cggccggcaa atcaaagcgg
     1441 gcgcgtatca gaaaatcgac aaggcgcaaa tcccccatta cggcaacatc gataaagatt
     1501 tgctgaaaat gatggaagcc gtcgatccgg gcaacgaata cgccgtcccc tatttctggg
     1561 gcatcaacac cttggcaatc aacacgcagc aggtgaaaaa ggcattgggt acggacaagc
     1621 tgcccgaaaa cgaatgggat ttggtgttca aacccgaata caccgccaag ctcaaatcct
     1681 gcggcattag ctatttcgac agcgcaatcg aacagattcc cttggcgttg cactatttgg
     1741 gcaaagaccc caacagtgag aatcccgaag acatcaaagc cgccgtcgat atgatgaaag
     1801 ccgtccgggg cgacgtgaaa cgcttcagct cttccggcta tatcgacgat atggcggcgg
     1861 gcaacctgtg tgccgccatc ggttacggcg gcgatttgaa cattgccaaa acccgtgccg
     1921 aagaagcggc aaacggcgtg gaaatcaaag tattgacccc gaaaaccggc gtgggcgtgt
     1981 gggtggattc ctttatgatt ccgcgcgacg cgcaaaacgt tgccaatgcc caccgctata
     2041 tcgactacac gctccggccc gaggtggcgg cgaaaaacgg cagcttcgtt acctacgcgc
     2101 ccgccagccg tcccgcgcgc gagctgatgg atgaaaaata cacctccgac gcatcgattt
     2161 tcccgaacaa agaactgatg gaaaaaagtt tcatcgtatc gcccaaatcc gcagaatccg
     2221 ccaaactggg cgtgaagctg tggcaagggc tcaaagcggg caaataaccg gaatccctgc
     2281 cgtctgaaac ctttcgggcg gcaggaaacg gcgcgtccgt tatcaaacag ggggcgtttc
     2341 cccgcccgcc tgttatgatt gggttaagat taaaatgatt tagtaaaatg agaaagatat
     2401 ggatttaagt atcgtagttc ccatttataa tgtcgagagt tatttggaag cgtgtctaaa
     2461 ttccatagaa cctatattaa gtaatgaaaa tgtcgaactt atccttgtga atgacgggtc
     2521 aaatagacgg aagtgaagat atatgttata aatatataga taaaacaaca aacaacaaac
     2581 aacaaacaac aaacaacaaa caacaaacaa caaacaacaa acaacaaaca acaccagata
     2641 ccgaacacca aacatcaaat atatatatca ggataaccaa gggacggcag aagctaggaa
     2701 taccggaata aaaaattcaa acggaaaata tatagtcttt attgattcag atgattttat
     2761 taattgtcag gtcttgctgg attttcttag taaagatgat attgatatgc cggatgtggt
     2821 ttttttaaat gcggttaaat atgataaggg aagtgtttca cattttggcg aagattatca
     2881 gcctgaaaaa atactcaagc aatccaaagt cgaagttttg aaaggattat gccgatttag
     2941 aaaatttccg ggttcggcgt gtaataagat tataaaaagg gaattgatta tcaaagaaaa
     3001 actgtttttt gaaaaagagg tttatgccga agatatcgaa tggtcaatga gattatttaa
     3061 tgcggcaaca actttttctt atttggacgg ttgttattac tattatcggc agggaagaaa
     3121 agattctaaa acaggaactg tttcggaaaa aagtataaag tcattattat atattttgga
     3181 gaaaaatgcg gaaatggaat ttgataggga tatatcaagt tatctttatt cttttctttc
     3241 ctacgaatat ctcgttttgc tttttatcat gacgagtaaa aatatagagt gtgatgctga
     3301 tataaaaaga agggcgtatc atttaaggtt tatgctgtta aagtccaata aattgatata
     3361 taagctgata ttcccgataa tcacattatt cggggtcgat attacaggca ggattttaaa
     3421 agcaatcagg gggaatattt aataaatcct ttaacactat acatcttacc gaaggaggaa
     3481 aaatgaacgc aatccgaact ttccaaaacc gcacgcccga aatccacgaa agctgtatga
     3541 tagacgaagc gtgcgtcgtc atcggcgaag tgtcgcttgc cgaagacgtt tctgtgtggc
     3601 cgtgcgccgt gttgcgcggc gatgtgaaca gcatcaccgt cggcgcgcgc agcaatatac
     3661 aggacggcag cgtcctgcac gtttcccaca aaaccgccgc caaacccgaa ggatcgccgc
     3721 tggttatcgg cgaagacgtt accgtggggc acaaagtgat gctgcacggc tgccgaatcg
     3781 gcaaccgcgt cttggtcggt atgggcagca ccgttttgga cgatgctgtg attgaggacg
     3841 aagtgatgat cggcgcgggc agcctcgttc cgccgcgcaa acgcttggcg ggcggctacc
     3901 tttatatcgg ttcgccggtc agacaagtgc gcgtgctgac cgatgaggaa aaagcctttt
     3961 tgaagtattc cgccgcgcat tatgtgaaac tgtcgaaaca gtacgggatg tgaaatcaca
     4021 tcggcgttcc tacgtcagcc ccaaattcat gcggacggga tgcccgataa cggtatccga
     4081 tgcgccttga ttttgaccgg ctgcgtttga gttgcaggca aaaatgccgt ctgaaagcct
     4141 tttttcgggt tcagacggca ttttatcgcc gattgctttt taaagtttga ccgaatgctc
     4201 gcgcgtttcg tggaacacga tgtccggcca acgttcttgc gtcagcccta aattcatgcg
     4261 gacgggatgc ccgataacgg tatccgatgc gccttgattt tgatcggtgc atttgagttg
     4321 caggcaaaaa tgccgtctga agcccttttt tcgggttcag acggcatttt attgccgatt
     4381 gctttttaaa gtttgaccga atgctcgcgc gtttcgtgga acacgatgtc cggccagcgt
     4441 tcttgcgtga gtcccagatt cacgcggtta ggggcgaggt aggcgaggtt gccgcccgcg
     4501 tcgatggcga ggttgcctgc attggctttt tcaaattccg ccagtttttt cttgtcgtcg
     4561 cacgataccc agcgcgccga ccagatggat gcgctgtcga acacggcttc tacgccgtat
     4621 tcgttggcca gccgcgaggt aacgacttca aattgcaaca cgccgaccgc gcccaaaatc
     4681 aaatccgcgc cgctcatcgg tttgaacacc tgcaccgcac cttcttcgcc aagctgttgc
     4741 aaaccttttt gcagttgctt gattttcagc gggtttttga tgcgaacgct gcggaacagt
     4801 tcgggcgcga agaatgggat gccggtaaac gtcagttgtt cgccttcgga gaagctgtcg
     4861 ccgatttgga tgttgccgtg gtttgggata ccgataatgt cgccggcgta ggcttcttca
     4921 accagctcgc ggtcgtggga catgaaggtt accacgctgg aggcggcgat ttcgcggttg
     4981 atacgcaggt gtttcatttt catgccgcgc tcgaatttgc cggagcagac gcgcaagaag
     5041 gcaatacggt cgcggtgttt cgggtccata ttggcttgga ttttgaagat aaatccggaa
     5101 aacttcggct cgtccggctc gaccatacgc acggtcgcat cgcgtggttt cggcgcgggc
     5161 gcccattcaa tcaatgaatt gaggatttcc tgaataccga agttgttaat cgcagagccg
     5221 aagaatacgg gcgtgagttc gccggcgagg aattcgtcga gattgaactc gttggaagcc
     5281 gcctgcacca attcgatttc gtcgcgcaac tgttggattt ccaatggaaa gcgttgttcc
     5341 aattcgggat tttctatgcc tttgatgatg tcgaactcgt gcggcaggcg ttcgccgccc
     5401 gcttcaaaga gatagatttc gtcgttcagg atgtggtata cgcctttgaa gtttttgccc
     5461 ataccgatcg gccaggtaac gggcgcgcag cggatttgca ggatgttttc cacttcgtcc
     5521 aagagttcca gcgaatcgcg cacttcgcgg tcgtatttgt tcataaacgt aacaatcggc
     5581 gtatcgcgca ggcggcagac gtttaagagc ttgatggttt gcgcttccac gccttttgcc
     5641 gcgtcgatga ccattaatgc gctgtccacg gcggttaaaa cgcggtaggt gtcttcggag
     5701 aagtcttggt gtcccggcgt gtccaagagg ttgacggtgt ggtctttgta gtcgaactgc
     5761 atcacgcttg atgccacgga aatgccgcgc tgcttctcga tttccatcca gtcggaagtg
     5821 gcgaatttgc cggttttttt gccttttacc gtacccgcgc tttgaatcgc acctgaaaac
     5881 agcaagagtt tttcagtcaa cgtggtttta cctgcgtcag ggtgggagat gatggcaaac
     5941 gtgcggcggc ggcgcacttg gtcgaggatt tcttgggaca tggttttctt tgcaaaaagg
     6001 ttcaggccgc ttttcagacg gcccggacag tgtttgagac ggcgaaattg tacaaaaaaa
     6061 tgcctgataa ttcaatgttg gaggcggtca gtgcgtgctg ccgtaaatct ctttttcgtc
     6121 tttcaggacg gcatcggcgg tttcccacgc gctgccacgc cagactttgt aaaagcagct
     6181 ttctcgcccg gtgtggcagg cgatgccgcc gttttgggcg atgagcatca caatggcgtc
     6241 gccgtcgcag tcgaggcgca gttcgcggac tttttgcgtg tgtcccgact cttcgccctt
     6301 catccattgt ttttggcgcg aacggctgta atagtgggca aagccggttt cgacggtttt
     6361 ttgcagggct tcggcgttca tccacgccac cattaaaaca cgtttggttt cggcatcttg
     6421 ggcgatggcg caaaccaaac ctttttcgtc aaatttgacg gcttcaagca ggtttttatc
     6481 catatttcct ttcagacggc atagtcgggg cggtcagagg cgcacttcga tgccggcttc
     6541 gcgcatagcg cgtttggctt cgcggatggc gatttccccg aaatggaaaa cgccggcggc
     6601 aagtacggca tcggctttgc cttcggttat gccttcaatc aggtgccgga cattgccgac
     6661 cccgccggag gcgatgacgg ggatgtcgac ggcttcggca acggcgcggg tcagcggcag
     6721 gttgaaaccc tgtttcgtac cgtccctgtc cataccggtg agcaggattt cgcccgcgcc
     6781 gcgtttttgc atttcgaccg cccattccac cgcatccaaa ccggtcggat ttcgcccgcc
     6841 gtgggtaaag atttcccagc gtgtgttttc ggggttggcg gctttggcat cgacggcggc
     6901 gacgatggct tgcgaaccga aaaatccggc ggcttcgtca attaaatcgg gacgggtaac
     6961 ggcggcggtg ttgatgctga ctttgtccgc gccggcattg agcaggcggc ggatgtcggc
     7021 aacggtgcgt acgccgccgc cgacggtcag ggggatgaag acttgtccgg caacctcttc
     7081 gatgatgtgc aggatggtgt cgcggttgtc ggatgaggcg gtgatgtcga ggaaagtcaa
     7141 ttcgtccgcg ccttcgccgt tgtagcgttt ggcggcttcg acggggttgc ccgcgtcgcg
     7201 caaaccgatg aagttcacgc ctttgacgac gcgcccgtct tttacgtcga gacaggggat
     7261 gatgcgtttt gccagtgcca taatcggatg cctttagtcg agggaatctg ccagttgctg
     7321 cgcttgggca aaatcgatgc tgccctcgta aatcgcgcgg ccggtaatcg cgcctgctac
     7381 gccatgtttt tcggcggcac acagggcgcg gatgtcgtcc aagccggtca gtccgccgga
     7441 ggcgatgacg ggaatgcgga cggcttgggc gagtttgacc gtcgcgtcga tgttcacgcc
     7501 gctcatcata ccgtcgcgcc cgatgtcggt gtagatgatg ctgttgacgc cgtcgtcttc
     7561 aaagcgtttt gccaaatcaa ttacatgatg cccggttacg gttgcccagc cgtcgatggc
     7621 ggccataccg tctttggcat ccagcccgac gataatcctg ccggggaagg ctttgcacgc
     7681 ctcgcgcacg aagtcggggt ttttgactgc cgccgtgccg ataatcacgt cgtttaagcc
     7741 caaatccaaa tattgtccga tggttttcaa atcgcgtatg ccgccgccga gctgtacggg
     7801 gatgtctttg gcgacggcgg cgaggatgtc tttgatggcg ggcaggtttt gcggaacgcc
     7861 ggcaaacgcg ccgttcaaat ccaccagatg caggcggcgc gcgccttgtt tgaaccagtg
     7921 cagtgcggtt tcggcgggcg aatcggaaaa gacggtcgcc tcttccatca gcccttgttt
     7981 caggcggacg cagcgtcctt ctttcaaatc gatggcgggt atcagcagca tagtttttct
     8041 ccttgtgcgg ggccgtgtcc ggcttaccag tttaaaaagt ttttcaacat cgtcagcccg
     8101 gcatcgtggc ttttttcggt gtgaaattgc gtggcgaata cgttgtcttt gccgacgatg
     8161 caggcaaacg gggacgggta gtcgctttcg cccaatatgg tttcgggatt ttcggggtcg
     8221 aaatagtagc tgtggacgaa gtaaaaacgc gtgtcttggg ggatgccttg aaaaagcggg
     8281 tggttttggg tttggcgcac ggtgttccag cccatatgcg ggactttcag acggcatccc
     8341 tgcgggtcga ggaggtcgcg ctcaaagcgt ctgactttgc cgccgaacca gcccaagccg
     8401 tcggtgtttc cttcttcgct gtggtcgaat aaaagttgcg cgccgacgca gattccgaaa
     8461 aacggtttgt tttttaaggc atctttgact gcctcgtcca aaccgtctcg ttttaatgcc
     8521 gccatacagt cgggcatcgc gccctgaccg ggaaaaatga ctttgtcggc gcgggacacg
     8581 cggtcggggt cgccgcttaa aaagatttcg gtattttttc cggcaagctg ccccgccgtc
     8641 cggacggatt tcaatacgga atgcaggttg cccataccgt aatcgataat ggcggtttgc
     8701 atggcttcct cctctttttt tgcaatatgg ctgcgatttt aacaaacaaa tgtgccgtgc
     8761 tgataaaaat gccgtctgaa aacgggagtc tgtcttcaga cggcataggg tttaaacccg
     8821 gaaagccgtt tgtcagcctt ccatttgttt tgcctgaacg gcagtcaggg cgatggtaaa
     8881 cacgatatct tctaccagtg cgccgcggga gaggtcgttg accggtttac gcaggccttg
     8941 cagcagcggg ccgacgctta agacgttggc gttgcgttgg acggctttat aggtgcagtt
     9001 gccggtgttc aggtcgggga agaccaaaac ggttgcctgt cctgccaccg ggctgcccgg
     9061 agctttggat ttgcccacgc ccggcacggt tgccgcatca tattgcagcg ggccgtcgat
     9121 ggcgaggtcg gggcgttttt cccgggcaag tttggttgct tcgatgacgg tatcgacatc
     9181 ggggccgctg ccggagttga cggtggagta ggaaatcatc gccactttcg ggtcgatgcc
     9241 gaaggctttt gcggaatcgg cagactggat ggcgatgtcg gcaagctgtt gcgcggtcgg
     9301 gttcggatta accgcgcagt cgccgaagac gaggacttgg ttgggcagca gcataaagaa
     9361 tacgctggac acgaggcttg cgcccggtgc ggttttaatc agttgcaaag cggggcggat
     9421 ggtgttggcg gtggtgtgaa ccgcaccgga taccaaaccg tccacatcat tttgcgccat
     9481 catcatcgta ccgagtacca cggtgtcttg cagttgcttg cgcgcgtctt cgggtgtcag
     9541 gcctttggat ttgcgcagtt cgcacatcgg ctcgacgtat tgttcgacca atgaggcggg
     9601 atcgatgatt tccaaagagt cgggcaggct gataccgcgt tctttggcaa cggcttcgac
     9661 ttcttcgcgt ttggcaagca ggacgcagcg ggcaatgcct ttttcgtggc agatggcggc
     9721 ggcttggacg gtgcggggtt ctgcgccctc aggcaggacg atgcgtttgt cggcttggcg
     9781 ggcgaagtcg atcaggttgt agcggaattg cgccggcgac aggcgttttg cttcgcggcc
     9841 tgccaatacg gatacgtctt tcagcgcgtc gctcgaaccg aagaaggtca ggccggtttt
     9901 ttcggctgcc gcttcggcaa cggaggctgc cgcgccgtcc acgacaaaac cttccaatac
     9961 gcccggcgcg gcggcgaaga actgtttggc aaggttcacc cgatttgcca gttcgtcggc
    10021 atcggcgttg tcggaacgga cggcgaagac ggctgccgcg tcaagggaca atgccagttc
    10081 gacgtttttg cctgcgaggt agattttgtc ggcatcgggc gcgaagcctt cgatgacgag
    10141 gttggcagca tcgagtgcgg caactttgcc gaccagtgcg tcgaaccagt cgtcgctttt
    10201 gccttgcgcg agcagggttt cggcggttgc gtcaacggct tggaaaattt gtgcgtccag
    10261 tgcttttgca aaggcttgtg cggcggcgga ggcgtccagt ccggcagata cgggtacgat
    10321 gagtactttt gccatgatat atcctttcgt atgctgcggt gtgcggcata tgtggttgga
    10381 aggggcggca tataggcaga aacggctgcc tgcgtgccgt gcgtgccgtg tttggcttga
    10441 ggcgcgcagg ttgaatatag caaacaaatt ctgtttccaa caagataaat atccgcaggc
    10501 ttgtggatgc tgccgccttt cagagggtat ttccggggaa gaacagggcg ggaccgtcca
    10561 aatggaggac ggcggaaatg ccgtctgaca gggtgggggc ggaagggagg ttgagcgtga
    10621 ggacggtttg tccgacccgg agactgattt cggtatgccg cgctttgggc gtggttttga
    10681 gaaccacggc gtgaatggag gcggtgggtg cggaatgggg gtgaaggctg aactgttccg
    10741 gacggatgag cagtgtgccg cgtgtgcctg cgggtgcgcc gctttgaacg ggcaggcggc
    10801 ccaatccgca atcggcggtg ccgtcggcgt tgagcgcggc ggggaacacg atgccttcgc
    10861 cgataaacag ggcggcatca aggtcggcag gttgtcggta caattcgtga gggcttgcag
    10921 tttggaggat gcgcccctgt ttcatcacgg caatccggtc ggcgtattgc agggcttctt
    10981 cgcggtcgtg gctgacgaaa acggcagatt tgccgttggc gcgcagggcg gcaatcatgt
    11041 cttcgcgaat ctggcggcgc aactgttcgt ccagcgcgct gaagggttcg tccaacaaaa
    11101 tcagttcggg atcgggtgcg agggcgcggg cgagggcgac acgctgttgc tgtccgcccg
    11161 aaagttcgtg cggacagcgt ccggcaagtt cggaaatgcc ggtcaattcc aacatagctt
    11221 cgatgcgctg ccgctcttgc gccgtcttgc ctttgccgtt gcccagcccg taggcggtgt
    11281 tgcggtaaac ggtcaggtgg gggaacagca caccttcctg tacgacataa cccaaacggc
    11341 gttcgcggac ggggaggttg gtatttttcg agaagatggt tctgccggaa agcgaaattt
    11401 cgccggaatc gggttgttca aaaccggcaa ggcagcgtaa aagggtggtt ttgccgcagc
    11461 cggacgcgcc gacgataaag aggatttcgc ccgggtcgag gctgagcgaa atgtcgttta
    11521 aaaccggggt gttttgaaaa cttttggaca ggtgtccgat gtgcagggcg gcggtcatgg
    11581 cggtacttcc tcaagctgtt atttgaaggc gtatttcttc agcaggaata cggggatgcc
    11641 ggaaaataat accagcatca gcgcgtaagg ggtggcggcg gcgtattgtg cgtccgatgt
    11701 gtattcccaa acggcggtgg agagtgtgtg gacatcgtcg gtggtcagca gcagggtggc
    11761 ggtcagctct ttcatcagtt tgaggaagac gagtgcgaat gcggcggtaa tgccgggcag
    11821 gatggacggc agtaccaacg tcctgaaaat aaagaagtgt ccgcgcccca atgttgcgcc
    11881 gacctgttcc atcccttttg ggagttgttc caaggaagtc ctcagggtgg tttgcgccat
    11941 cggcaggtaa agcatgaaat aggcaaggat gacgacgata aaggtttggt aaacggcagg
    12001 ggtgtagttg atgctgaaat aaaccaagga tagggcgata accaaaccgg ggacggcgtg
    12061 cagtaaaaac ggcagcctgt ctatccaaac ggttaaaaaa ttgcgatagc gaaccgatgc
    12121 ccaaacaagg ggcaaggcac ataatatagt caaaatcgca cctaaagccg atacgcttaa
    12181 ggaacggata aaggcatcaa atacggatac gagcgcgaat gtgccggaag tgccgaccat
    12241 catccaatgt atcaatacgc caaaggggat aataatgccc aaagtcaaca agctgcttaa
    12301 aaaaacaatc gcgccaatct gaccgggcag tttgagggtt ttgacgggat aaggacgggc
    12361 aacgcctttg ccgctgtggt aaatcttggc tttgccgcga aatatgcttt ctccaaatac
    12421 gacgatgccg cacaccgcca ttaaaacagc ggaaagcagg gcggcggtat tgttgttgta
    12481 ggacatttcg tattcttgga aaatggcggt ggtaaaagtg gggtagttca aaatggatac
    12541 cgcgccaaat tcgaccagca tatgcagggc aattagtaac acgctgctgc cgatggcggg
    12601 tttgagctgg gggaggatgg cggaaaaaaa ggtttgcagg cggcttttgc ccaaggacag
    12661 gctgacttct tcgtaagaca ggctgatgcg tttgagtgcc gcctcgacgg gcaggtaggc
    12721 gagcgggaac gaggacaggc tcataatcat cactgtcccc caaaagcctt cgacacggaa
    12781 ggtcaggctg atccaggtga aacagctgac aaatgcgggg atgcacaaag gcagggtgat
    12841 tgccgtctga aaaaaggttt tgccgaagaa gcggtaacgt tggaacaaaa gggcgcaggc
    12901 aatgcccaaa acaatggaaa tcagggtaac gcccgccatc atcgtcaagg tgttggagag
    12961 caaatcccac atacgcgggc ggaacaacag ttcgacggcg cggttgatgc cgacctgcca
    13021 cgaacgcata gcgacatata aaaaaggcag ggtaagcggt agggcaatca gtaggatgag
    13081 gccggtaagc caaatgggta tttttttagg agacatagtg tttttcatcg gcaaaacggg
    13141 cggacagtat aaatgtccac ccgtttgaca atccgaaaac ggcttatttc ataccggctt
    13201 gctcaagcag ccgggtggcg tgttcttttt cggaaacagt ggtggcggac acttggggtg
    13261 cttccaactt ggcgatgggt tccaaattga aggtggatac cacgtgcgga ttcaaaggat
    13321 actcggcacg gacggcggtc agggcacgct gtccttcctt gccggcgagg aaggcgacga
    13381 attttttcgc ctcatccttg ttttgggagg attttaacac ggctgcaccg gaataggtaa
    13441 tgagtgcgcc gggatctctg tggcggacga aattcaggcg ggtgtggacg ttttgtacgc
    13501 ctttttcacg cgcgaaagcg tgccagtagt agttgttgat gagggcggca tcgatttcgc
    13561 cgttttcaac cgcttgaagg gcgacggagt ttttagcgta aggcttgccg tattctttca
    13621 gacctttgag ccatttcaat gcggccgctt cgcctttcag tttgacgatg gcgacaacct
    13681 gttccaagaa cgcgccggaa gtgggggcgt aaccgatgcg gtttttccat ttcggcgtgg
    13741 cgtaattcag gacggatttt tctaaatctt tttcagacag tttgcgggtg tcgtaaacga
    13801 cgacgcgcga acgtccgctc agtgccaccc agtctttttt ggcggcaacc ggcacgccct
    13861 tgccgcgtgt ttcgttgatg gtggaggcgg gcaggggctc taggaggttg gctgcggaaa
    13921 gggtggcgag tgccgggatt tgttcggaat agaatacgtc ggcggggctt cggctgcctt
    13981 cttctttgat ttgaccggca agctggtcgc ctttggcgct gttgagtttg actttgatgc
    14041 cggtagcccg ggtaaaggca tctgcaacgg cttgtgctgc ttctttgtgt tgtccgttgt
    14101 acacggtaat gtctgccagc gcgggggttg cggcggtcag ggctgcggca agcagtgcgt
    14161 atcggataga tgttttcata tcgattttct cctaatgaat gagagtgtat accttgttaa
    14221 gacataacgg tgtgtagtgt attccttctt ttttataaat gcaaataatt attttttaaa
    14281 tttgttgttg tccgatccgg ttattgtttg ttctgacttg tattttttcc gtgagtctcg
    14341 cccgtaaggc ggaagtggcg ggcaatgcgt ggcggaatgt gggtaaaggc ggcattttga
    14401 tttgtcggaa tgcttgagaa cccctctctt taaaacaccg tctgaacagg gttgctggaa
    14461 tagtattgcc atcccagcag atacagtttg tcggggtctt gccaatattg ttcatccaga
    14521 ctgttcggca gtgaggcggt tttgttgtcg agatgttata tcccacattt ctttcaggtt
    14581 tttaccttcc gattggaggc ggcggatttc tttgtccaat gcgtctgccg cccgatggat
    14641 cagcattgcg ctgtgtatcg cgccgatttt tttcagaacg gaactagtct tttcggcggc
    14701 gggaaaacac cagttgcaga caaattgcag tatgccgccg ttaccgatgt cggcttccgc
    14761 ccgccaaatc aaaaccagct cctgttcccg tccgtccatg ctttcgagtt tgccgttatg
    14821 ctgttcaaaa agtttgtcca ccgcctgcca catcatctgc tcgaagcggt cggcttgggt
    14881 atccgtatcg gtcatcgtgt tcttgccttt taaaaatgcc gtctgaacat ttcttcagac
    14941 ggcatttggg ggttaagcca acatttcccg ccagcgtttc acttggaagc ggacttgttc
    15001 cggcgcggta ccgcccaagt ggttgcgggc gtttaagctg ccttcgggtg tcagcacgcc
    15061 gtaaacgtcg tcggcaatca aatcgctgaa accttgtaag acttcgagcg gcagttcgct
    15121 caaatcgacg cccgcttggt cggcgtggcg cacggcttgg gcgacgactt cgtgggcatc
    15181 gcggaaaggc atgccttttt tgaccagata atccgccaag tcggtggcgg tagcgaagcc
    15241 ctgcatcacg gcggcgcgca tattgtcggg tttgacggtt acgccgcgca tcatatcggc
    15301 gtaaatccgc aacgtgtcga taagcgtgtc ggcggtgtcg aacaagggtt ctttgtcttc
    15361 ctgattgtct ttgttgtacg ccaagggttg ggatttcatc agggtaatca gaccgataag
    15421 gtgtccgatg acgcggccgg atttgccgcg cacgagttcg ggcacgtcgg ggtttttctt
    15481 ctgcggcatg atggacgaac ctgtgcagaa acggtcggcg atgtcgataa agccgaaacg
    15541 cggactcatc cacaaaatca attcttcaga caggcggctc aggtgaacca taaccagcga
    15601 ggcggcggcg gtgaactcga tggcaaaatc gcggtcggat acggcatcga gcgagttttg
    15661 gcagatttgt tcaaagccca acagttcggc ggtgatttcg cgccgaatcg gataagtcgt
    15721 accggcaagg gcggctgcgc cgagcggcat acggttgacg cggcggcggc agtccgccat
    15781 ccgttcgtta tcgcgtccga gcatttcgac gtaggcgagc atatggtgtc cgaagctgac
    15841 gggctgggcg acttgcaggt gggtaaagcc tggcatgacg gtttcggcgt tttgttccgc
    15901 caaatccagc aatgccgtct gaaggctttg aatcaggctt cggatgacgg taatctgatc
    15961 gcgcagccac aggcggatgt cggtggcgac ttggtcgttg cggctgcggc cggtgtgcag
    16021 gcgtttgccc gcgtcgccga ttttgtcggt caggcggcgt tcgatgttca tgtggacatc
    16081 ttccaaatcg gacgaccatt cgattttgcc gctgcggatt tcttcgagga tttccgccat
    16141 gccccgacgg atgtccgcca aatcgccttc gtccaacacg ccggtttctt tcaacatttg
    16201 cgcgtgggcg agcgagcctt gaatgtccca ttcggcaagc cgtcggtcga aactgatgga
    16261 ggcggtgtat tgtttgacga gttcggaaac gggttcgttg aaacgtccgg accaggtttt
    16321 gtcgtgcata aggattcctt gatggggtta ttcggtgcgg tatttttcca aaaggcggcg
    16381 gaagggttcg ccggtttcgg ggtgtttcag accgtaggcg acggtggctt cgaggtagcc
    16441 cagtttgctg ccgcagtcgt agcgcgtgcc ttcaaagggg tgcgccagga caaattcgtg
    16501 atcgagcagc ttggcgatgc cgtctgtaag ctggatttcg ttgcccgcgc cgcgcggcag
    16561 tccggtcagt aagtcgaaaa tgcgcggggt aaggatgtag cgtccgacga cggcaaggtt
    16621 ggagggcgcg tcttcgggct tgggtttttc gacaatgccg ataatgcgtt ggaactgttt
    16681 gagctgttcg gtttcgacga tgccgtatga gccggtttgc gatgcttcaa cggtttctac
    16741 gcccaaaatg ctgttgccgc tgcgcccgta cacttcgacc atttgtttga gcgcgccttt
    16801 gggggcatca atcaggtcgt cggcaaggat aacggcaaag ggttcgtctc cgatggcggc
    16861 gcgggcgcac aagacggcgt gtcccaagcc cagtgcttcc gcctgacgga tgtagaggca
    16921 ggtaatgttc ggcggcagga tgttgcggac gtgttccaac aatttgtctt tatggcgcat
    16981 ttccaactcg gtttcgagtt cgtatgcctt gtcgaaatgg tcttcgatgc tgcgtttgtt
    17041 gcgtccggta acaaacacca tttccgtgca gccggcttcc acggcttctt ctacggcgta
    17101 ttggatcagc ggcttgtcga cgatgggcag catttctttc gggctggcct tggtggcggg
    17161 caggaagcgt gttcccatcc ctgcgacggg gaagacggct ttccgtatgg gtttcattct
    17221 ttttcctttg tattgttttg atgtttaaag ggcgagtttg cggattaaat cggcgagtgc
    17281 ctgcgcgcgg tggctttcgc ggtttttgac ctccgaatcc aattcggcgg cggttttgcc
    17341 gtgttcgggc agataaaaat acgggtcata accgaaaccg ttttgccctt gcggggtatc
    17401 gtgccactgt ccgtgccata cgccttcggc aataatcgga cgcgggtcgt tttcatgacg
    17461 gataaaaacc aatacgcaga cgtagcagca gcttttgtcg gctttgcagg caagttcgcc
    17521 gacaagtttc aggttgttgg cggaatcgga tttggggttg tcgccggcgt agcgtgcgga
    17581 atggatgccc ggcgcgccgt ttagggcggc ggcacagatg ccgctgtcgt cggcgagtgc
    17641 gggcagcccg ctgtattcgg cggcatggcg tgcttttgcc agcgcatttt caacaaaggt
    17701 ggaatagggt tcggggcatt cgggtatgct gaactcggat tgcggcaata cggtaatgcc
    17761 gtatggttcg aataggttgc cgaattcttt gagtttgcct gcattgccgc ttgccaaaac
    17821 gattttttcc ggtttttcag acatagcggt tttcctttgc ggcggattgg gcggcgcgta
    17881 gggatttgtg ccgcaggtag agggcgaggc tgccgatttg tccgaacagt gcgccgaatg
    17941 cgaaaagaaa ggaggcgacg gcgaaggctt tgctgccgac ggtcagcaga taagcaccca
    18001 agaggacgag cagcataaat gccagtgtga agagggcaac agacaacaga tagatttttc
    18061 ggcggttcat ggcgttcggt cggaaacggt atgttcggat tatagccgat tgggacggta
    18121 ttccctagag cttggaaaaa tgccgtctga aggggcgttc agacggcatg gcgggcgtta
    18181 tgcctgtttg ttccaacgtt cgatggattc tttgatgact tttttcgctt cttccgcatc
    18241 gccccaagat tcgactttgg tcgtacctgc ttttttcagg tctttgtaat ggttgaagtg
    18301 gaactcgatt tgtttgatga gctgttgcgg caaatcggac aaagttttgt aggcgttgcc
    18361 gttgttgcgg tcgtcggcag gaacgcagac gattttgtcg tctacttcgc cgtcgtcaac
    18421 gaatttcatc acgccgataa cgcgcgcttc caagaatacg ccggttgcca gaggttgttc
    18481 ggtaacgagc agcacgtcca attcgtcgcc gtcttcgtcc aaagtttggg gaatgaagcc
    18541 gtagttggtc ggtttggcga agatggcggg ttcgacgcgg tcgagttgga atgcggcgag
    18601 tttgcggttc cactcgattt tgtggttgct gccggcgggg atttcgttga cgacgttgat
    18661 gatgccgccg tccacgtcgc cggtggtcag gatttggttg aagtctgcca tttggtttcc
    18721 tttatttggg aaagtttgaa gtttgaaagt atagcacaaa cgtccggctg gaaatgcgcc
    18781 cgatgccgtc tgaaagggtg tacgggcgcg tgttaccgtt tgcccaaaaa cctgcccagt
    18841 tccagaatcg cgcacctgtt ggacgaggaa aacacttttt ccgccgcttc ttccatatcg
    18901 aaccagccgt aggagacgtg ttcctcgggt tgcaggacga cgggcgtatc gcgcgggatt
    18961 tcggcagaga agaggtgttc gcggttttca aacacgcctt ttggatagcg gtgccgccag
    19021 tggtggtaga tttcgtaaac cgtgctgtcg tgccagtctt gaagctgccc gtccgccagc
    19081 aggatgccgg tttcttccca aacttcgcgc cttgccgttt gtgcgacggt ttcgcccggt
    19141 tcgaggctgc cggttaccga ctgccaaaat cctttcggat gcgtgcgttc gatgagcagg
    19201 atgccgccgt ccccgctata aaggacgacc agtgcggaaa cggggtattt gagcggtttt
    19261 gccatcggca tctttcggcg ggctgcggta atgaaggggc ggattatagc aaacgccgca
    19321 cgttatggcg tttatccttt tccgtatcct tttttctgca cggatgggac gcgccggtgt
    19381 ttgccggtaa attttccgtt gtgtcaaaaa gataagggcg gttgtgattt taatgcttgc
    19441 caaagcgtcg ggcggaaact ataatccgca acttatcgag tcggagtgtg gcgcagtctg
    19501 gtagcgcact tgcatggggt gcaaggggtc gaaggttcga atcctttcac tccgaccaaa
    19561 aattccgaaa gccgctttca aaagcggctt ttttgccgtc cgtatgatta tgatgtagag
    19621 tacgcggcga cagacattca aatgccgtct gaaaaccgtt cagatggcat ctctttatct
    19681 tagtttcatt ccgtaccatc ttaaggaaca tcaaattggg catttcccgc aaaatatccc
    19741 ttattctgtc catactggca gtgtgcctgc cgatgcatgc acacgcctca gatttggcaa
    19801 acgattcttt tatccggcag gttctcgacc gtcagcattt cgaacccgac gggaaatacc
    19861 acctattcgg cagcaggggg gaacttgccg agcgcagcgg tcatatcgga ttgggaaaca
    19921 tacaaagcca tcagttgggc aacctgatga ttcaacaggc ggccattaaa ggaaatatcg
    19981 gctacattgt ccgcttttcc gatcacgggc acgaagtcca ttcccccttc gacaaccatg
    20041 cctcacattc cgattctgat gaagccggta gtcccgttga cggatttagc ctttaccgca
    20101 tccattggga cggatacgaa caccatcccg ccgacggcta tgacgggcca cagggcggcg
    20161 gctatcccgc tcccaaaggc gcgagggata tatacagcta cgacataaaa ggcgttgccc
    20221 aaaatatccg cctcaacctg accgacaacc gcagcaccgg acaacggctt gccgaccgtt
    20281 tccacaatgc cggtagtatg ctgacgcaag gagtaggcga cggattcaaa cgcgccaccc
    20341 gatacagccc cgagctggac agatcgggca atgccgccga agccttcaac ggcactgcag
    20401 atatcgttaa aaacatcatc ggcgcggcag gagaaattgt cggcgcaggc gatgccgtgc
    20461 agggcataag cgaaggctca aacattgctg tcatgcacgg cttgggtctg ctttccaccg
    20521 aaaacaagat ggcgcgcatc aacgatttgg cagatatggc gcaactcaaa gactatgccg
    20581 cagcagccat ccgcgattgg gcagtccaaa accccaatgc cgcacaaggc atagaagccg
    20641 tcagcaatat ctttacggca gtcatccccg tcaaagggat tggagctgtt cggggaaaat
    20701 acggcttggg cggcatcacg gcacatccta tcaagcggtc gcagatgggc gcgatcgcat
    20761 tgccgaaagg gaaatccgcc gtcagcgaca attttgccga tgcggcatac gccaaatacc
    20821 cgtcccctta ccattcccga aatatccgtt caaacttgga gcagcgttac ggcaaagaaa
    20881 acatcacctc ctcaaccgtg ccgccgtcaa acggaaagaa tgtgaaactg gcaaacaaac
    20941 gccacccgaa gaccaaagtg ccgtttgacg gtaaagggtt tccgaatttt gaaaaagacg
    21001 taaaatacga tacgagaatt aataccgctg taccacaagt gaatcctata gatgaacccg
    21061 tctttaatcc taaaggttct gtcggatcgg ctcattcttg gtctataact gccagaattc
    21121 aatacgcaaa attaccaagg caaggtagaa tcagatatat cccacctaaa aattactctc
    21181 cttcagcacc gctaccaaaa ggacctaata atggatatag tgccgggaat ttaagcaaaa
    21241 aagtcctgac tttagtttac ataaacggca aactcaaatt cgggccaagc agtctgccgt
    21301 cttcggcatt gaaagcacct gttcccattg tcggcaaggc tgaggctccg cccggaaaag
    21361 ctgtgcctgt cggcgaagcc cgcattcctt caaatccaaa agcggtaaaa ggaacaaccg
    21421 ttaccctgtc gcaggaacaa cgcctagcca acggagagct gataccgaaa ggctctgtgg
    21481 taacgtggtc ggacaatgcg gtgaaggttg tgcggcctga tgggaaaaag cggagattta
    21541 atagtcggga ttgggaacag aaggcattgc cgaaaccgca actaccgaaa gattacagag
    21601 atgacatcaa cataggtgga aataatccta gatttaatca gcaacggcat gaaaaaatcg
    21661 aatcccacaa cccaaacaga atagaggaga tacgccaacg taatgccgtt tcttctgcag
    21721 aaaggcaggc tgatgcatta aaaaggatgc aaacgtctgg ccgcacccgc ctgcctgggc
    21781 agccacaaaa aggaaaaccc ggcgctgcta gagaaatgtc ctcttcagac aatccgaatc
    21841 aaacagccga ggcttttgca cgtcgatatt taggcaggaa acctacacta atggaacgag
    21901 tcgaaggtga aaaattatac ggaggtaaat gtgtgggctg ttggaaagcg caaatgccgg
    21961 acggaacctt tgtggtgtat aggccagcag gtaatgcttc tatgaccaag ggaactactg
    22021 cgtctgttga tattaataac ccaacattaa ataacttaaa tattaattta aaaggcaagc
    22081 caacagagtt aaaatttaaa ttccctgcta gataaggatg ttttaatgaa tttagagaca
    22141 gcagaaaata ttcaatgcga atttgagtat ttagcattag atggttattt tccaatgcat
    22201 tttgcatcgc atggtcaagg aaacaaagat tggcaatttg cagttgaatt catatataga
    22261 ttattaatat gtcaattagc aactcttgaa cctatcaact ttgaaacgaa aaatgatatt
    22321 ttagactttt gccataattt agcaaaacaa agtcctttta ataatgataa tgaggtgtgg
    22381 tatcaggggg aaattgtatt aacaaaaaaa gggatagatt taataaagga atatattcca
    22441 gaggcgtttg aagagtggaa tggaaagaaa tttgaattaa acattccttt cattaaaaca
    22501 ttaaaaaata tatttgttaa ttatgaggta gcatgggatg agaataatcc actattcccc
    22561 attatatctt taaacctcgg cacctgataa acatggggtt tatcaagcga cagtggaaat
    22621 taaaaagcct gatggaagtt gggaaatgaa aaaaggaagg ggcggaaaac ctatgacaaa
    22681 tcattctatg ttcccaaaag attgggatga ggctagaatt agggctgaag ttacttcggc
    22741 ttgggagaaa agaaaggaat tagaaggtca aaaatggaga ggtacaagta aatctggtgt
    22801 tgaaatagaa ggatttacgg aacctaatcg aacagcttat ccactttata atagtaaatg
    22861 aagtattgat taaaaaggtg tttttaaatg tataaaataa aatgttttta tgatcagttt
    22921 agttctggtg aattatttaa ctactgccag tttttagata attcttctaa tcaaaagata
    22981 aatacacgag gaacggtgta tcaatatatt atttatgttt taacaggaga cctttatcta
    23041 caaaaagaca tagatgaaaa tttagaattt atccatcaag cagaaaataa tcccaatgaa
    23101 gtttattcag gaggtggaca aggtttttgc tgggatattt ctgctgaaaa agtagttttc
    23161 tatcacaacg aatttgatga agaagacggc tggcctgatt taagctgttc gctgcatact
    23221 tttaaaactg ctttaattgc ttggaatgct tttttgcaat tgcctaaaag tattcattcg
    23281 gtggtggaga ctgtgattga ggaataagca taattagctt aatgaataga atcagcgata
    23341 tagattggac tgcaaatcca cgcttatacg ctgtgccatg attaacagga agagagcaac
    23401 ttggatgggc tagtagggat ggtaagcatt taaatatatc aattgatgga aagattacac
    23461 acaaatgact aatttaaaat tagatttcta ctctgaagtt attataaaag attcttgccc
    23521 taatgatttg ttagaaaatg gtgagactat taaaggaaaa aaaggagtgg tattaggtat
    23581 aagcgaagaa gatggtataa tctatggcta tactatttta ctttttgata taaaatattg
    23641 tatatatata gataagaaat atattatacc gactggaaaa aattctctcg cgatgatttt
    23701 tattagctaa tttaagtaat ttaagcaagt tttttagata ctgaaactgg taattatgtt
    23761 tatgtaagca taaggagttc agattgaagg attctacagg gcaaatgagg ctggcaacca
    23821 aggatttggc ggaagccatt aaacgaggag aagtacgtag ttctgctttt acaacaaagc
    23881 aactaaaggc aatcgaaaaa ggagaatcta aaataccgga ttacacttgg catcatcatc
    23941 aagatacagg gagaatgcag cttgtgcctg aatgggaaca ttctaaaacc ggtcacatag
    24001 gagggacggc aatggggaag ggtaaataat tatgtggaaa atttataaag aaaatagcac
    24061 cgacttaaat tttgccatag gcagtatata ttgccaagca atcaatctta ccgaatttga
    24121 aatgtgggtc gaaaaaatca taagggaagt ggatttagat gaaattccaa attatttttt
    24181 tgatttgata gattttcaat cactatacga tctgattgat attataggat ttgttcccga
    24241 aaataattta tcaaaaaatc aggataatgc attaaccggt attgcttttt taagggggat
    24301 agatgtctat gaccctcccg tttcaaaaga aaaagcatta aaagccttag aaaaacatcc
    24361 tgaaatttat cagaggtttc agcatttctt cccatttgta gagctgcccc cgctttaaac
    24421 agtcaaaatg ccgtctgaaa caatattcgg ctttcagacg gcatttttga tacaaagcag
    24481 gtaaccaaaa gagcgtttga cggcaaagga agataattat gtggaaaatc ataaaagagg
    24541 atagtgatga tttagaattt gcaattaaat gcttattctc tcaatctatt gatttaaatg
    24601 aattcaaatt atggattgaa caagtaatac gcgatatgcc cattgaggac atcccttttt
    24661 atatttttga tttggcggat ttggatgaag aaattgataa tatttacaat atcataggtt
    24721 ttgtttcgag ttacagctta tcaaaattga gaagaaatgc cttaacaggc attgcattct
    24781 taagggggat agatgtctat gaccctcccg tttcaaaaga aaaagcatta aaagccttag
    24841 agaaatatcc tgaaatttat cagaagtttc agcatttctt tccatttgta gaactgcccc
    24901 cgctttaaac agtcaaaatg ccgtctgaaa gccatttccg ccgctcagac ggcattttcg
    24961 cccctttcgt ttacaaaccc ttaaaatcca tttacactca aactcgttca aaacaaaaca
    25021 aaccctgcca tgaaaa
//
LOCUS       DAXHRI010000014        23633 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXHRI010000014 DAXHRI010000000
VERSION     DAXHRI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.014"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(191..1966)
                     /gene="ptsP"
                     /locus_tag="ACFM75_000413"
     CDS             complement(191..1966)
                     /gene="ptsP"
                     /locus_tag="ACFM75_000413"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC6255007.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIAKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(1966..2235)
                     /locus_tag="ACFM75_000414"
     CDS             complement(1966..2235)
                     /locus_tag="ACFM75_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC6255008.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2304..2741)
                     /locus_tag="ACFM75_000415"
     CDS             complement(2304..2741)
                     /locus_tag="ACFM75_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC6255009.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2822..3385)
                     /locus_tag="ACFM75_000416"
     CDS             complement(2822..3385)
                     /locus_tag="ACFM75_000416"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC6255010.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3454..4278)
                     /locus_tag="ACFM75_000417"
     CDS             complement(3454..4278)
                     /locus_tag="ACFM75_000417"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC6255011.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQTKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     IGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            4370..5002
                     /locus_tag="ACFM75_000418"
     CDS             4370..5002
                     /locus_tag="ACFM75_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC6255012.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGSHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNHWLEEGETLKVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5070..6860)
                     /gene="rmuC"
                     /locus_tag="ACFM75_000419"
     CDS             complement(5070..6860)
                     /gene="rmuC"
                     /locus_tag="ACFM75_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC6255013.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAHLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAYLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(7199..7987)
                     /locus_tag="ACFM75_000420"
     CDS             complement(7199..7987)
                     /locus_tag="ACFM75_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC6255014.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDTYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8002..9351)
                     /locus_tag="ACFM75_000421"
     CDS             complement(8002..9351)
                     /locus_tag="ACFM75_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC6255015.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDVLGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9370..9951)
                     /gene="petA"
                     /locus_tag="ACFM75_000422"
     CDS             complement(9370..9951)
                     /gene="petA"
                     /locus_tag="ACFM75_000422"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC6255016.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10074..10823)
                     /locus_tag="ACFM75_000423"
     CDS             complement(10074..10823)
                     /locus_tag="ACFM75_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC6255017.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            10954..11883
                     /locus_tag="ACFM75_000424"
     CDS             10954..11883
                     /locus_tag="ACFM75_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6255018.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPIGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSKLEPV"
     gene            complement(12039..12431)
                     /gene="rpsI"
                     /locus_tag="ACFM75_000425"
     CDS             complement(12039..12431)
                     /gene="rpsI"
                     /locus_tag="ACFM75_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC6255019.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIEFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12441..12872)
                     /gene="rplM"
                     /locus_tag="ACFM75_000426"
     CDS             complement(12441..12872)
                     /gene="rplM"
                     /locus_tag="ACFM75_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC6255020.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13050..13229)
                     /gene="ssrS"
                     /locus_tag="ACFM75_000427"
     ncRNA           complement(13050..13229)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFM75_000427"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13235..13540)
                     /locus_tag="ACFM75_000428"
     CDS             complement(13235..13540)
                     /locus_tag="ACFM75_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC6255021.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13551..13880)
                     /locus_tag="ACFM75_000429"
     CDS             complement(13551..13880)
                     /locus_tag="ACFM75_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255022.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(13935..14924)
                     /locus_tag="ACFM75_000430"
     CDS             complement(13935..14924)
                     /locus_tag="ACFM75_000430"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC6255023.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15047..17749)
                     /gene="ppc"
                     /locus_tag="ACFM75_000431"
     CDS             complement(15047..17749)
                     /gene="ppc"
                     /locus_tag="ACFM75_000431"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC6255024.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVTELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQISPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGNPDTLTALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            17795..18688
                     /locus_tag="ACFM75_000432"
     CDS             17795..18688
                     /locus_tag="ACFM75_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6255025.1"
                     /translation="MPHPCQNVHTLTALFPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIERQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFDEGDVGKLKTEALADRLHHINHTVDVRTVNEKLDSGRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            complement(19088..19312)
                     /locus_tag="ACFM75_000433"
     CDS             complement(19088..19312)
                     /locus_tag="ACFM75_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC6255026.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIARLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(19313..20701)
                     /locus_tag="ACFM75_000434"
     CDS             complement(19313..20701)
                     /locus_tag="ACFM75_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC6255027.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(20787..21608)
                     /gene="prmC"
                     /locus_tag="ACFM75_000435"
     CDS             complement(20787..21608)
                     /gene="prmC"
                     /locus_tag="ACFM75_000435"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6255028.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(21598..22059)
                     /locus_tag="ACFM75_000436"
     CDS             complement(21598..22059)
                     /locus_tag="ACFM75_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255029.1"
                     /translation="MQEQNRKPSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            complement(22124..23608)
                     /gene="tldD"
                     /locus_tag="ACFM75_000437"
     CDS             complement(22124..23608)
                     /gene="tldD"
                     /locus_tag="ACFM75_000437"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC6255030.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILTGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT         5109 a         6565 c         6284 g         5675 t
ORIGIN      
        1 aagtttctgt gccgacaaac ctagattccc gcctgcgcgg gaatgacgga ttttaggttt
       61 ctgattttgg ttttctgttt ttgagggaat gaccgatttt gggtttctgt ttcggttttc
      121 tattttgcaa gaatggcaaa atttcagatt gcgggcattg ttaagtattt ctatttttta
      181 cctgccgtat ttatttccgc cccttgaagt cggcttcttc ctcgacagac acgctgttca
      241 tctgtttgat cagcttttcc gacttctctt cgtcttcgca gcggatgact ttcgcaatat
      301 cgctttcgag ctgtccgaca ttgctgtgca gaatgatgtt tttgacgggc aggatgttgt
      361 tggggttcat ggaaaaacgg cgcagcccca tacccaataa aacgcgggta aacgcggtat
      421 cgcccgccat ctcgccgcat acggatacgt ctttgtccat gcggttggcg gtacggatga
      481 cgtgttgcag cattttcagc acggcgggat ggccgggctg gtagagatgg ctgacgctgt
      541 cgtcgccgcg atcgacggac aagatgtatt gaatcaggtc gttggtaccg acggagatga
      601 aatcgaccag tttcaaaata ctgccgacgg tcagcgcggc agacggaatt tcaatcatac
      661 agccgatgcc gactttaccg aaggcatcgc cgcgttcggc aagctggcgt tgcgcggtgt
      721 cgaggtggat gaggcactgg cgcacttcgg atacggaggt aatcatcggc cacatcatgc
      781 ggacgggacc gtgtaccgcc gcacggagga tggcgcgcat ctgggtgcgg aacatgaccg
      841 gttcggcaag gcacaggcgg atgccggtca tgcccagcgc ggggttgagg ctgccgttgg
      901 gcgtgctgtt tttcccgaac cagcgcgggt ttttgtccac acccaaatcg actgtccgta
      961 tcgttacgct tttgcctttc atttttttga caatcgcgct gtacacttcg tactgctcgt
     1021 cttcagacgg catcgtatcg cggttcaggt aaagaaactc gctgcggaac agcccgatgc
     1081 cgtctgcgcc gaggttgtgc agcggtttca cgtcttcggc ggattctata ttgcccacaa
     1141 gctcgatgca gaccccgtcg gcggtggcgg cggcggtttt tttgagcttg ttcaaatcgc
     1201 gtttgtggct gcggtattcg cgggcacggc ggcggtattc gttcaacacc gactcatccg
     1261 gcgcgataat caacacgccg ttgataccgt ccacaatgac cgtttcgcct tcggtaatca
     1321 gtttgcgcgc gttgtgcagc ccgacgacgg acgggatgtc caagctcctg cccaaaatcg
     1381 ccgtatgccc ggtggggccg ccggcatcgg taacgaaggc ggcaatgcgc tgctctttaa
     1441 acaaaaccgt gtcggcgggc gaaaggtcgt ttgcaatcag aacggtttcg tcaaacaggt
     1501 tgtcggcaac ttccaactcg ttgccctgcc cgatcaggtt gttgtggatg cggcggacga
     1561 cttgcagcat atcctgcttg cgttcgcgca aataggcatc gtccatattg tcgaattggg
     1621 cggcgagttt gtcgctctgc tgcttcaatg cccactcggc gttgattttt tgttccctta
     1681 aaatatcgac gggttcgcgc gacaaggtaa catcggtcaa gagcatcagg tgcagcgaga
     1741 tgaacgcgcc caactcggtc ggggcgtttt cgggaatcgc gctgcggagc tgttccaact
     1801 ctttgcgcgt ggctttgacg gcggcatcga aacgttcggc ttcggcatcg gtgtccgcct
     1861 ccgcaacatc atactgcggc acttcctccg taccgcgcgc aatcaggtgg gcgcaaccga
     1921 cggcaatgcc tttgcccgcc gccacgccgt gcagcacgat actcattatt cgccctcgcc
     1981 gaagtagccg ttgattaagt cggtcagggc gcgcatcgct tccgcctcgt ccgcgccgtc
     2041 cgtctccagt tcgatgaccg tacccttggc ggcggcgagc atcatcagcc ccataatgct
     2101 tttgccgttg acgcggctgt cgtttttcgt aacccagact tcgcttttga attgggacgc
     2161 ggtttgggtg aacttgttgg acgcgcgggc gtggagtccg agtttgttga tgatttcgat
     2221 ggattgtttg agcatttcgg ttcccgtgtt atgtatatcg gcagcagacg ccgtttaaaa
     2281 tgttttcctg ccctgccgct tcttcagacg gcatcgccgc tgcgccggca caccaaatct
     2341 tcgggcgcgg acgtgatggc gaaaatgcct tttacagccg cctccctgac gcattcggta
     2401 aaggcggcaa ggtcttccgc cgccggcgaa tattggacgg ccttaaccat catcggcgcg
     2461 ttcagcccgg tcaaaatcgc cgatttgttt tcgcgcacga ggcggcgggc tgcattgcag
     2521 ggggtcgcgc cgaaaatatc ggtcataatt agcacgccgt cgttgtcggg aaactcctga
     2581 agcgcggcaa tggcgttgtt gatgatgtcg tcttggtctt ccgtcggctg cacgccgagt
     2641 atgcggacgt tttcaggcag tccgcccgga aaaaaatgat gtgccagctt gcggtaggct
     2701 tcgcctatgg tttcgtgtgt gatgattaaa agccctatca tattatgcgt cctgttcctc
     2761 gttgtcctgc cggcgtatgg gcgcaatgcc gtctgaacag ccttcagacg gcatcgcgtc
     2821 cttattttcc gtccaatgcg taaatctcgc ccaaattgcg ccagcagccc gccgcatcca
     2881 tgccgtaacc gaaaacataa cggttcggca catccagtcc gacataatcg gctcggatag
     2941 gcttgggttt gtcaatcagc ttgttggcga acaccgccgc acggcagctt gccgcaccca
     3001 tttccaaaag tttggcttga atggcggaca tcgtatgccc ttcgtccaaa atatcgtcca
     3061 gcacgacgac gtgcctgccc cggatttgtt ccgcatcggg catacgcttc cagttgaacg
     3121 cgccgccctc cagcttgtcg ccgtaacggg aaacgtgaac ataatcaaaa tctaagggaa
     3181 aacgcaacag cggcagcaac tgccccgtaa acaccaccgc gccgcccatc acgggcagca
     3241 gcagcggata tttgccgccc aaatcgcgcg taatctcgtc cgccactttt tgcagtgcgg
     3301 cacggcattg gccttggtcg aacaaaagat cggcgttttc aagcatcgcc tgtgtttcaa
     3361 ggcgtttggt ttctaaatcg gtcatatgtc ggaatcggtc ggtaaaagga aaattataaa
     3421 ccaaagtatc ggatgccgtc tgaaccgtcc tgctcagcgg tcggtacgca cgcgcacaaa
     3481 tgtggcaaat ttcggcgtgc ctttccgcgt aaaaccacgg tagcggtagg taatcagtgt
     3541 gccgattttg ggcgggttgt cgcggtcttt gtctttgaaa ccgctgccga tgcggaattc
     3601 gccgtgccgg tttttgcagc cgatcgcgcc caaccgtccg gcgtttcgcc ctttgccctc
     3661 atagtgccgc gttaccgtgc attcgtcgtc gtattggctt ttcagcttca ataattggct
     3721 gctcctgccg ccgctgtaac gggattcggg ctgacgcagc atcacgcctt cgccgccctg
     3781 cgcttcgatt tgttttaaaa agtccatcgc gtgctgccgg tcgcgcactt tggtttgcgg
     3841 gatgatggta atcggcgcgt tcggatgcgt tttcagccac tgcgttgcga ctgccaaacg
     3901 ttggtagaga ttgccctgcg ccttgggtac atcgaaaacg tgcaggcgga tgccgcgcca
     3961 gtctgaagaa acagaacgca cggtagcgga aatctgctcg aactgaccac gtccgctata
     4021 caattcgccg tccaaaggat aaggcggaaa ctgcgcggta aagcctttgg ggggcgtaaa
     4081 cgcgtagccc tgacggctta tcaggtgctt tccgtcccaa taggcgcgca cgccgtcgag
     4141 tttctcgctc attgcccagc cggcaatatc ctgccctttg tattcctgcg ccagcatcaa
     4201 atccgccgcg cctgccgatg cagggatgaa aaccgccgta aaaatcggta tgatgccgcc
     4261 gattgtcttc ttaatcatct gattccccaa atatcaaaac aggcggcaaa ccgccataaa
     4321 acaaacggca aacccgatgc cgtctgaaaa accgtttagg aacacgccga tgaccctacg
     4381 ttacgaaatc ttccccgtta cccccttccg ccaaaactgc accctgattt gggacgacga
     4441 aagcggcgaa gccgtcctga ccgatgtcgg cggcgacgtg ccgttcctgc tgcaagcgtt
     4501 ggcaaaccgc aaacttacgc tcacggcaat ctggctgacg cacggccatc tcgatcacgc
     4561 gggcggcgtg gtcgaaatgt tgaaaacgca taaagtccct gtcctcggtt cgcaccgcga
     4621 agatgaattc ctgctccaat cgctgccgca aaccaccgcg caatacggat ttcccgtctc
     4681 gcccgccttt gcgccgaacc attggctcga agaaggcgaa acgctcaaag tcggacgcta
     4741 tgcctttcaa gtgctgcata ttccggggca cacgccggga cacgtggttt tctattgtgc
     4801 cgaagcggag ctgctgattg cgggcgacgt gctgttttac gaaaccatag gcagaaccga
     4861 ttttccgcgc ggcaaccacg ccgacttaat caataatatc cgcaacaaat tattcgccct
     4921 ccccgaaacc gtgcaagttg tcgccggaca cgggcgtatg acttccatcg gacacgaaaa
     4981 acggcacaat ccgtttttct aaccgacttc actacggtct tcagacggcg tcatctgcac
     5041 tgatgccgtc tgaaacgcat ccgcctttcc tattcggctt cgtcagccgc ttcctgttcc
     5101 gaaggtttgc ccaaagcaaa tgccgtctgt tcgttggcac gctcgaccaa atcccgttga
     5161 agctgtttgc ctgccttcac gcccaacaga cgcagtttct cggcgcggcc gaccagattc
     5221 ccgcgcccct cggcaagttg cttgaatgcc gtctgaaagc tgttttgcgc ctgatcgatg
     5281 cctttgccga cgctttcgag cgtctgtaca aagccgacaa atttgtcgta cagcttgccg
     5341 ccttcgtccg caatcgccag cgcgttttgg ttttgctgct cgttgcgcca aatattcgcc
     5401 accgtccgca aagtcgccag cagcgtactg gggccgacca gcataatccg tttgtcgaaa
     5461 cactcttgga acaagccagc gtcattctgc aacgccaaca ggtaggccgg ttcgacaggg
     5521 ataaacataa agacgaaatc caatgtgttc acaccttcca aatcggtgta atccttcagc
     5581 gacaagcctt tcatgtgtgc acggatgctg gcaacgtgtg ccgccagttc gcgtgccgcc
     5641 gtatccgcat ccgccgcctg cgtgtagcgc acataagctg tcagcgagac cttggaatca
     5701 atcacaatct gcttgttgtc gggcaggttg accaaaacgt cgggctggag gcggcgcgtg
     5761 ccgccgtctt cctcttttcg gacggatgcc gcctgaacca catattcgcg ccctttctgg
     5821 aggccggaat tttccaaaac cgtttccaga atcatttcgc cccaattgcc ctgaaccttg
     5881 ttctgcgtac cggtcagcgc gttggtcagt gcctttgcct cgctgtgcag ctgcgcattc
     5941 aatccttgaa gccgtttcaa ttcgttttcc aacgtcagcc gctcgcgcga ttctttatca
     6001 taggtttgct tgaccaactc gccgaaaccg tggatgcgtt cgttcagcgg gttcaaaacc
     6061 tgatggagct gctcccggtt ctgctcggta aaacggcggc ttttttcttc caaaatcgtg
     6121 ttagcaagat tttgaaactg atcgctcaaa ctcttgcgcg cctcgcccag caaggacagc
     6181 ttctcttcag aagcaaggcg ttcctgttcg atttgcgttg ccaaacgttc gttttcaacc
     6241 gccaaaccct gtgccttttc ctgcaactcg gtatgcgact gcctcaaccg ctccgcttcc
     6301 gcctcttttt cctgcaaatg ggcaatctgt ttttcggctg cggcaaaacg gttgcccaca
     6361 tcggaaaggt cgttttgcac gtcgcggaca gtttggcggc tttcttccaa atcggtttcg
     6421 actctttggc ggatttggcg ttccagggca tattggtcgg caaatgcctt ccgctcctgt
     6481 ccgagctgcg ttgccaaacg ttcgttttca accgccaaac cctgtgcctt ttcctgcaac
     6541 tcgatatacg actgcttcag acgcgccgac tccgcctctt tttcctgcaa ataagcaatc
     6601 tgcttttcgg ctgcggaaaa acggttgccc aaagcataat tttcgtcctg caaatgccgg
     6661 tatttcccgt ccaacaccgc caattccgac acggttttgc cgtgtgcctg ttcgacaaaa
     6721 tcacatcttg ccgccttttc cgccaggtgc gcgttcaaac cggcaaactc gccctgaaac
     6781 ctgcccttca tcagcaacca tgtaaacaac acgcccgaca ccaacgccgc caaaggcagc
     6841 aaaacagtca tcagttccat caatcatcct aatattcaaa cattttcaca ccggacaaaa
     6901 ccgccgctta ttccgattct acctgtttgt tcgacatagc tccaaaaaat atagcggatt
     6961 ggctttaaac ctgttcgaca tcgccttacc atgctgcttg cggtttcaga ccttttccta
     7021 attcaatatc aatctgccac aaaccctgat taagttcccg atgtctgaca tttttagaat
     7081 gatgccgtct gaaatgttgc agctatgttc agacggcata cggattcagg cttttcaaac
     7141 ggcaggcaaa atgaaaaaag ggcaaaccct aaaggatttg cccttttgtt ccaaacgctt
     7201 agtgtacgtc tttccaatat tcttttttca ggaaatacgc caaaggcagc ataaccgcaa
     7261 ataggaaaat catcacgaca tagcctatac gtttgcgttg cagttgtgca ggttcgccca
     7321 tgtacacaag gtaattgacc aaatcgcgta cataagtatc gtactctttt tggatcactt
     7381 tgccgttagg caggcggcgg ctgtgcaaac cggtagattc ccaatacagc ttaggcttca
     7441 tctcgccgtg ttcgtctttt accataaccg gctgaccttt ggcatccaac tcaacggctt
     7501 gaacgccttg ctgttcccac aacgggtggg gcataccgac tttatcgaat acagtattgt
     7561 tccagccgct cggacgggtc ggatctttat agaagccgcg catataggcg taaaggtagt
     7621 ctgcaccttt ggaacgcgca atcaacgtca aatcgggcgg agcagcacca aaccattttg
     7681 ccgcatcttt cgggttcatc gccgaatgca tgacatcgcc gacattatcg gtggtaaaca
     7741 tcaggttttt cttgatttct tcgtcagtca aaccgatgtc tttcaaacgg ttgaaacgca
     7801 taccgcttgc cgagtggcag gacaggcaat agttggtaaa gatttgtgcg ccgcgctgca
     7861 ggctgacttg gtcgcgcaaa tcgatatcga ctttctcata gtgtgcatga ccgcccgaag
     7921 caaatgccgc acccatgggt actgccaaca gcaaggcagc aaaccaattt ttcatagctt
     7981 gtttcatttt gactgccctc atcagatgtt ggttgcaaac aggtaagcac ccacggcggt
     8041 aatgccgacg taaacaaaga acatgatttt ttgtttggcg gtgctcatgg ttacgcgttc
     8101 gggaaccggt ttgtttttat ccagcttggt atagaacggc atacccagga agaatgcgaa
     8161 atagacgaag gacaggatgc gcgcgaccaa agtacgcgta tcggttgcca ccatcgcgcc
     8221 caaaataccc aaaccgataa aggaaatgat gaacaagacc aatgcggttt tgaaaatcgg
     8281 gccgcggtaa cggacggatt tcacctcgcc cctatccagc caaggcagca gggcaatcaa
     8341 cacgaccgcc gcgcccatac cgattacacc ccaaacctgc gttcccaaga acgagggaat
     8401 cgcacgcaaa atggcgtaga acggtgtgaa ataccatacc ggcgcaatgt gcggaggtgt
     8461 tttcagcgca tttgccgcat cgaagttcgg cgcttcgagg aagtagccgc cgccttccgg
     8521 tgcgaagaac aatacggcac agaagacaat caggaataca accacaccca agacgtcttt
     8581 aacggtatag taaggatgga atgggatacc gtcgcgcggg ataccgtttt cgtctttgtt
     8641 tttcttgatt tcaacgccgt cagggttgtt agaacccact tcgtgcaggg cgatgatgtg
     8701 cgccacaacc aagccgagca ataccagagg taccgcgata acgtgcaggg caaagaagcg
     8761 gttcagggta acgtcggaaa cgttgaagtc gccgcgaatc caagtggaca aatccggacc
     8821 gataacaggg atggcggaga acaggttaat aattacctgc gcaccccaaa aggacatctg
     8881 accccaaggc agcaggtagc ccataaaggc ttccgccatc aatgccaaga aaatcagaga
     8941 accgaaaatc cacaccaact cacggggttt tttgtacgaa ccgtaaatca aaccacggaa
     9001 catgtgcaga taaacaacaa tgaagaagaa agatgcgccg gtggaatgca tgtagcggat
     9061 aatccaaccg ccggacacgt cgcgcatgat gtactcgact gcagtaaagg cagcaggcag
     9121 atggtaggcg ttcaggttgc cgtcgggttt gtagttcatg gtcaggaaaa tgccgctgac
     9181 gatttgaatc acgaggacaa gcagggccaa agagccgaag tagtaccaaa aattaaagtt
     9241 tttcggtgcg tagtattgag ccaaatgctc attccacatt ttagacaggg ggaaacgagc
     9301 gtccatccag cctaacaatg cttttgcttt gctattggtt tggtttgcca taattatcgt
     9361 tccttattct tagtcttcgc ccaccaagat agttgtgtcg ctcaagtatt tatatggcgg
     9421 gacaaccagg ttggtcgggg caggaacacc tttatatacg cggccggcca agtcgaattt
     9481 cgaaccgtgg caggggcaga agaagccacc tttccagtct gcacccaaat cggcaggggc
     9541 aatgtcggga cggaaggtgg gcgagcagcc caaatgggtg cagataccga tggcgacaag
     9601 gatgttcggc ttaatcgaac gggtctcgtt tttcgcatac tccggctgct gttccgcatc
     9661 ggaattggga tcggtaagtt cgccgttcag gcctttcagg tctttaagct gctgatctgt
     9721 acggttgagc acccaaatcg gtttgccttg ccactcggcg gtcagcagct gacccgcttc
     9781 gattttactg acatccacct cgacggcagc accggcggcc ttggcttttt ccgaagggaa
     9841 aaaactggcc acaaacggcg ttgccacacc caatgctgcc actccgcccg cgccgcaggt
     9901 cgcgagtgtc aggaaacggc ggcggccgtt gttgatttct tgattatcca ttattcagtc
     9961 gtcctaatat tttgggaata ccgagccatt aaacgttgca attttaccca gtttgcagtg
    10021 atactcaaag cattatttaa aataaggtaa agttttatga tatttctcaa gactcaagcc
    10081 ggattgtttt cgtcaaaatg gcacacttcc aacccgaaaa cctctgccgc cgattctgcc
    10141 agcgcgcgta cgccgtaacg ttccgtcgcg tgatgccctg ccgaaatgaa agccgtaccc
    10201 gtttcattgg caaggtggta ttgggcttca gagatttccc ccgtcaaata cagatcgaca
    10261 ccttcatcta ttgccgtctg aaaaaaaccc tgcgccccgc cggtacacca tgcgacccgt
    10321 cggatttcgc gttcgggatt gccgataacg acaggcttac gttgcaaaac tgtttcaata
    10381 tgcgccgcca atgcgccgag tgtcttggct tgtttcaggc tgcccgagtt gagcaggttt
    10441 tgttcgccga accgtttttc tgtcgcaaaa cccaatctgt cggcgagttg ggcattgttg
    10501 cccagtgtgg gatgtgcatc caggggcaga tggtagcctg ccatattgat gtcgtgccgt
    10561 aacagtgcgg caatccgttc ttttttccaa ccagtaacgg tcggcaactc gtttttccag
    10621 aacataccgt gatgtaccaa aagcaaatct gccttctgct ccacagcaaa atcaatcgct
    10681 gccctgcttg ccgttaccga cgtaacgatt ttcccgatat attccctccc ttcaacctgc
    10741 aaaccgttag gggcgtaatc tttaaacaac gctgtctgca atgtttcatt acaccaagtc
    10801 agaaaatccc tgcgcaatac catctttttt cctaatcgct ttaaacaagc gggcattcta
    10861 atcgcaaaat gtccggaatt cacatttttc cgatttgcac ccgcatatga attattttaa
    10921 tatgcgccgg ttcaatatgc cgtctgaagc cccatggatt ccattatcga actgcgccac
    10981 ctcaagaccc tgctggcact tgaagaaacc ggcagcgtct cccttgccgc caaacgggtt
    11041 ttccttaccc aatccgccct ttcccaccag atccgtatgc tcgaaaacca ctacggcacg
    11101 ccgctgttcg aacgcaaatc cacgcccttg cgctttaccc cggttggaga aaggctgtta
    11161 cgcctcgctc acgacctgtt gcctcaagtt accgatgcag aacgtgattt ggcacgcatt
    11221 atagaaggag aggcaggaga gctgcggatt gccgtcgaat gccatacctg tttcgactgg
    11281 ctgatgcccg ccatgggcga attccgcccg atgtggcccc aagtcgaatt ggatatcgta
    11341 tcgggattcc aagcggaccc tatcggactg ctgctgcaac accgcgccga tcttgccatt
    11401 gtttccgaag cggaaaaaca aaacggtatt tctttccaac cgctgtttgc ctacgaaatg
    11461 gtcggcattt gcgcaccgga ccatccgctt gccgccaaaa acgtttggac ggcggaagac
    11521 tttatcgggg aaaccctgat tacctatccc gttcccgatg aaatgctgga tttacccaaa
    11581 aaaatcttaa ttccgaaaaa catcaaccca ccgcgccgac acagcgaact gaccattgcc
    11641 attatccaac tggttgccag ccggagggga atcgccgccc ttccctattg gacagtcatg
    11701 ccctaccttg aaaaaggcta tgtcgttcac cggcagatta ccgacgacgg attgcaaagc
    11761 aaactgtatg ctgccatccg cactgaagac acagataaaa gttatctgaa caacttttgc
    11821 caaatcatac gtgaacgcgg cttcgcggac ttgcccggat taagtaagtt ggaaccagtc
    11881 tgatttccct tgttcaaacc atacccatgc ggtttttcta ttttttcatg tactgttttt
    11941 gatttttgcc caatttgcaa tctttaaatt gccaatggga aaccgtctgc tacaaataaa
    12001 aaaccctgcg ataagcaggg ttttttgaat ttccaacatt aacgtttgga gaattgtttt
    12061 gcacggcgtg ctttgcgcag acccggtttt ttacgttcga cttcgcgtgc atcacgagta
    12121 acgaaaccgg cttgagacaa ggcgggtttc aacgcggcat cgaattcgat cagggcacgg
    12181 gtaatgccgt ggcggattgc gccggactgg ccggtttcgc cgccgccaac aacattgact
    12241 ttgatgtcga aagattcggc gttttcagtc agaaccaagg gttggcgaac aaccattcgg
    12301 ctggtttccc gtgcgaagaa ttcgtcaacg ggacgaccgt ttacgatgat ttgacctgta
    12361 cctttaatca ggaatacacg agccactgaa cttttgcggc ggcctgtgcc gtagtagtat
    12421 ttaccgttca tgtcgcgtcc ttatttcagt tccaaaactt tgggttgttg cgcagcatgg
    12481 gcgtgttccg cacccgcata cactttcagt tttttaatca tggcgtaacc cagaggacct
    12541 ttgggcagca taccttttac agcttgttcc aaagcgcggc ccgggaattg ctcttgcatt
    12601 tcgcggaagg tgcgttcgta gataccgcct gggaaaccgg aatggcggaa gtatttttta
    12661 tcttcgaatt tggcaccggt tacacgcagt ttgtccgcat tgataacaat gatgtaatcg
    12721 ccggtatcga cgtggggggt gtattcaggt ttgtgtttgc cacgcagacg gctggcgact
    12781 tcggccgcaa cgcgacccaa gactttgtct tgggcatcga tgacgaacca ttcgcgcttc
    12841 acctcgtggg gtttcgctga aaaggttttc atagtggaaa tccagataga tatagaaagt
    12901 tgtaaatttt aaagacagga ttcgattttg tcaatcgcat taccgcgtta cggaaggatt
    12961 ttccggattt cggcagactg catactgctt tttcgggcgg ggcggcggcc aatgtgaaaa
    13021 accgcatcgt tgcgatgcgg ttttgaatgg gaatccccgc gagagccgtt tcggccgaat
    13081 ccgcttgaac cttgctgaca aggcggctgc ctcgggtagt ttcgggtgcg tccgcaaaag
    13141 gacgctcgcg cccactactg ctcccggcaa ccttaagcga acttattggt tcaaaggaat
    13201 atatgccttc gcggacaccg cagggaaaaa ggggttattc ctgcgccaag cgggatagtg
    13261 ctttttggca ggcgttgtcc atatcggcta ttttacgcgc aaaatcgccg attgccaaat
    13321 cgccgccgtt cagagaggtt ttcaacaggt cgtggacgac gttgagcgcg gccataatga
    13381 cgattttttc gctgtccgcg acgcgtccgc cttcgcggat ggcttcggct ttgccgttga
    13441 gcattccgac tgcctgcaac agtgtgtctt tttcttctgc cggcgtgttg acggtcagcc
    13501 gggcgtgcat gacttcgatg tggacttgtt cgatgttcat cctttaatcc ttattgctgc
    13561 gtttcctgcc attgggggag gcgcgctgcc agtgcgctga ttttttccct gctctgttcg
    13621 agcaggctgc ggtatcgtgt attttcttct gtcaggctgt cgattttgtt ttgcaggtct
    13681 tctttgagtt tgccgacttg gacgagcagg gcttcgctga gttcgtcgac ggcggtttcg
    13741 tgttcgagtt tttgccgctc gtgcgcccgt ttgagttcgg cgacggtttc tttgaggcgg
    13801 cggttttcgc tgacgagggt ttcgaatttt tgtaccagcg tataaacgct gctttcgagt
    13861 ttttcgatat tttgtttcat aaccttacct gtccgtatgc cgtctgaagg cttcagacgg
    13921 catctgtctg ttgtttattc gaaacgcgcg ctgcgttcca tcagtctttc gacgacctgc
    13981 tgaggggtca tttccttacg gatgagttgc agcagggttt gggtaatcgg catatcgatt
    14041 tggtacttac aggcagtatt gaagacttct tctatcgtgc tgaccccttc ggaaacgtgt
    14101 ccgatttcga ccagcacctg atgcagttcc ttgccttctg ccaaacccaa gccgacgcgg
    14161 cggttgcgtg agagtgcgcc agtgcaggtc aagatgaggt cgccgatgcc tgccagcccc
    14221 atcatggttt tgggctgtgc gcccattgcg gaggcaaggc gggtaatttc agctaatccg
    14281 cgcgtaacca gtgcggcacg ggcgttaagc ccgtactcta ggccatcgga caatccggtg
    14341 gcaatcgcca taacattttt taccgcgccg ccaaccgcca cgccgataac gtcggtactg
    14401 ccgtaaagcc tcatgacagt cgtgttgagc tgcggtacga gttcttcaat ccactcttgg
    14461 ttttcggagg cgaggacgac ggcacagggc agttgtttgg cgagttcctg tgcaaaactc
    14521 gggccggaga gtacgccgat tttcttattg tcgggcaata cttctttcaa tacttgaaag
    14581 gtcagcagcc ctgtatcctg ctcgaatcct ttgcaggcgg cgaggacggg gaggtgtccc
    14641 gcgccgtact gtttgagcag ctctgcgctg cttctcaatc cggcaacgga ggttacgata
    14701 aggacaagtc cgctgtcttt gagcgcgtct gccaaatccg cacacacttc caaggtttcg
    14761 ggaaaggaaa agccgggcag tccgcgtttg ttttcacgcg cttcctgcat ttggcggact
    14821 tggtctgcgt tgcgcgtcca cagggatacg cggttgccgt gttgggaaaa gtgcagggcg
    14881 agcgccgtac cccacgaacc tgcgccgata acggtaattt tcattggtcg tctttcaaca
    14941 tatcactgcc gttcacttta aaacaatcgg tgtttctctg caagtgcggt cagggaaatg
    15001 ccgtctgaaa ggtattcaga cggcattttg tcccgatgtg gcactatcag ccggtattac
    15061 gcaaaccttg cgccacgccg ttgatggtca ggtgaaccat cagcagcgcg tgcggattgt
    15121 cgggttcttt acgcaggcgt ttgagcatag cgacttgcag gccgttgagg gcgttcaggt
    15181 aaggaatcct caaagcgagc gaacgggcga ggctgcggtt gtcgcgcaaa agctcttcgg
    15241 tttgcagcag gtcgagcagt gctttgcggc tgcggcgata ttcttcctta atcatcccga
    15301 agatgatttt tgccttatcg ggcgattcgc tcaagccggc atagttttcc gcgagggtga
    15361 tgtcggtttt cgccatcact tgctccatat tggagagcat ggcttggaag aacggattgc
    15421 tttgggcatg ttcgcgcagg gcggtcagtg tgtcgggatt gccttcgcac aaggtttcca
    15481 ccgcgctgcc gaaaccgtac caagcaggca gcatgaggcg gttctgcatc caggaaaata
    15541 cccacggaat cgcgcgcaag tcttgaatcc gcgctaaggt cttgcggctg gcaggacggc
    15601 tgccgaggtt gagggtggcg atttcctgaa tcgggctgat ttgcaggaag tagtcgatga
    15661 agtcggggtg ggtaatcagt tcgcggtagt atttgaacga tacgtccgac aatgcctgca
    15721 tcagtttggc atcagggtct tttttatccg gcaggatgct ggcttccaaa gtcgcggcaa
    15781 ccaaggtttc caagttgcgt tgggcattgc cggggtcggc gtatttggcg gtaatgactt
    15841 cgccttgttc ggtaatgcgg atttgtcccg ccacgctgcc ggccggttgc gccaaaatgg
    15901 cttggtaaga agggccgccg ccgcgtccga cgctgccgcc gcggccgtgg aacaggcgca
    15961 tgcggacatc gtattttttg aagagttcga ccaagcccaa ttccgcctga tacagacacc
    16021 atgagctggt aacgtagccg ccgtctttat tggagtcgga gtagcccaac atgatttctt
    16081 ggatattgcc gcggctttcg agcagggcat cgtaccagtc gaggcggaac atcgtttcca
    16141 taaccggaca ggcgttttcg agtgcttcga tggtttcaaa cagcggcacg atattgatgc
    16201 ggctgtgcgg tttgccgttt tccaccgcca acaggccgct ttctttcagc agcaatgcca
    16261 aggcgagcag gtcgctgggt tgttcgcagt tggaaataat gctttgtgtt acggcatctt
    16321 cgccaaattc gtctttgatt ttgcgcgctt cgttgaaaat tgccagttcg tggcgggtat
    16381 ggtcgctgta tgtgatgaac gggctgtaca gcggacgttg atggctcaat tctcgcaaca
    16441 ggacggcttg tttttgttct tcgttcaggc tgttgtagtc ttccaagcct gcgtgttgga
    16501 aaagctcggt aaccacatcg gcgtgtttgc ctgcgtgttg gcgcaaatca agcggcatca
    16561 tatggaagcc gaacacggat acggagcgga tgaggtctgc cagacggcct tcggcaagca
    16621 ggcggctgcc gttgtcgata agggaacgtt gcaatttttt caaatcatcg agaaattctt
    16681 gtgccgaagc ataaggctcg aggaagccga atttgcagcc catgcccaaa ccgagtgagc
    16741 gtgccttgcc catagcgcgt gccatgatgt aggcgatggc gcggcggtag ggttcttcgg
    16801 tacgggcgat ttcttcgtca ggcgagaggg cggccagtgc cattacgtcg tcgttgactt
    16861 tgacgcggcg gatggagagc ggcagttcgc ggtaaagttt gtcgagttcg ccacggtaaa
    16921 aacggaacac ggcatcggcg tggcggcgga aggcaaaacg caaggtttcg gcagaaacga
    16981 acggattgcc gtcgcggtcg ccgccaatcc agccgccgat tttgaggata ttcggaacgc
    17041 agacatcggg atagaccatc tgaaagtcgt gttccatatt gcggtagagc ttgggcaggg
    17101 cttcgaaaaa gctcatcggg aagatggaca cgccgttgtt gatttcgtcg ttgacgctga
    17161 gtttgtggcg gcgcgtttcg ctggtctgcc acaagcccag caggatagtg tcgatttcgc
    17221 ggcgcagccg tgccagcgcg tcggcattgg tgcagcgttc gcgttgcggc aacagtgcgc
    17281 ggatgcggcg gttgaagctt aagacggttt ggcgttgcac ttcggtcgga tgcgcggtca
    17341 aaacggcggt aacggacgta ttgtccaact gccgctgcac cgatttgccg tcggctttcc
    17401 ccgctttgag cctgcggacg gtttccgtca ggctgccttc cgcgccgccg cgtccggctt
    17461 cttcgtggat ttggcggcgg cgttcgtggt gcacgtcttc ggcgatgttc aaaatctggg
    17521 cgaacaggcc gcaggccaag gttaaatcgt gggtttgttg ttcgtccaat tgcggcaata
    17581 ctttttcaat caatgccgcg ctgtcgtcgg aagtggacag cagcttgacc gtttccacca
    17641 ccaacggcga ggcttcttcg tgtaggaggt taaacaggga ttgtttcaga aattccgcgt
    17701 ccgccgccaa agccgcgtcc ttcggattgt tcaggatatg cagttgcatg atttttctct
    17761 ctcgtttgcc gtaaatattg taaatgtacc tcaaatgccg catccgtgcc aaaacgttca
    17821 cactttaacc gcccttttcc cgaaatgccg tctgaagttg aacgccgccc gacggcagcg
    17881 ttacaatcgc ccgcaactgt tttttccgaa catcatcatg accacgaccg aacacgacaa
    17941 cgacgatgca ttcctgctgc ggtacagccg gcacatcctc ttggacgaaa tcggcatcga
    18001 aaggcagcag aaactttccg acgcgcatat tttggtcgtc ggatgcggcg gtttgggtgc
    18061 cgccgcactg ccctaccttg ccgcttcggg tgtcggcacg ctgaccatag ccgatttcga
    18121 cacggtcgaa ctgcacaacc tgcaacgtca ggttgcattt gacgagggcg acgttggcaa
    18181 actcaaaacc gaagccttgg cagaccgtct gcaccatatc aaccataccg tcgatgtccg
    18241 caccgtcaac gaaaaactcg acagcggccg cctgaccggt ttggttcaaa ccgccgatat
    18301 cgttttagac tgctgcgaca actatgccac gcgccaagcc gtcaaccgtg tctgcgtaca
    18361 aacgaaaaca ccgctggttt caggagcagc ggtgcgcttt gaagggcaac ttgccgtgta
    18421 ccgtcccgac ttgcccgact cgccgtgtta cgcctgcctg tttgacggcg gatcggcttc
    18481 agacggcatc tgttccctct tcggcgtgtt ctcgccgctg gtcggcatca tcggcagtac
    18541 ccaagcggcg gaggctctga aaatcctgct ggatgcgggc gaaccgtcgc acggcaggct
    18601 ggcggtttac cgtgccttgg aagggggctg gcaatatttc gacctgccgc gcaaccccga
    18661 atgcccgatt tgcggcgcgg aacggtaaaa ccctgccgcc gtttcagacg gtatccaaac
    18721 ggatgcggcg gaacggtttt aaaaatttaa aaatttacat ttctttgcaa aaaaaaataa
    18781 acttacctta taattgcagt tgttttagca atgtctgttt cgcagactca ttgagtaaga
    18841 cgttttcccc gtaatgtgtt tggccgtctg tcccctttgg gttcggacgg cttttttttg
    18901 gctgtgtttg aatacccggt tgatttcgtc tgtttgcagg gggaaagccg cttatttccg
    18961 ttcgggtgga aaacggttcc atcggataaa aggcattttg tccgactgat taaagttaca
    19021 tcttagagcc gcatcggcgg aatattcaaa cacagctttt ttaaggaaat ccggatacgg
    19081 cggcgcatca ataatgcggc ggaatctcgt cgcgcaggga atacggctct tgcgcgtcgg
    19141 gattcctgtc ctgcattttt tgatacagca gcctcaactg agcctgctgc aaatccagcg
    19201 tctgccgcaa ttccgccacc atcgcgttca ggcgggcgat tacgtcctcc tgaagcgcgg
    19261 attggatttc caattcgaca atacggcgtt ccaactcttg aaccgcgtcc atttacagca
    19321 ccattgcggc aatccagccg gcaatcagca gcgggatgtt gtagtggatg aaggtcggga
    19381 taacggaatc gcggatgtgg tcgtgctgcc cgtcggcgtt cagccccatc gtcgggccca
    19441 gcgtggaatc ggacgcaggc gaaccggcat cgcccaacgc ccccgccgtg ccgacaatgg
    19501 cgacggtggc aagcggcgaa aaacccaaac cgacacacaa aggcacataa atcgcggcaa
    19561 taatcggcaa agtggaaaag gacgaaccga tgcccatcgt taccaaaagc cccaccacca
    19621 gcatcgccaa tgccgccata cctttgctgt tgccgaatat cgccatactg ctttccacca
    19681 gcggctggat atgcccggtc gcattcatca cggcggcaaa accctgcgcg gcaatcataa
    19741 tgaagccgac catcgccatc atcttgatac cttcgccgaa tacgtcgttt gccttgtcgc
    19801 ggttgatgac ccccaacatc ataaatacgg cgaaaccgag catcgcgccc aacaccagcg
    19861 agtcttcata catcaactgg atggcaaaac atacggcaat ggcggcggcg gcggccaggc
    19921 tgcggtaggc ggacggctgc ggacggtttg ccgcatcggc gttgcccgcc gtatcggcat
    19981 tgttgctttg gtacaggcgc ggtttgcggt aatggacaaa cgccagcagg agtccggcca
    20041 gcattcccaa cgcgggaatc gccattgccg ccatcacgtt aatgtttttc acatcaagct
    20101 gcggcgcggc ggaatggatg ttgcccaaca ggatttcgtt caaaaaaatc gcgccgaagc
    20161 cgtaaggcag gaacatataa gtcgtaacca gcccgaaagt gatgacgcac gcaatcaggc
    20221 ggcggtcgat tttcaggcgg ttgaacacca aaagcagcgg cggaacaatc atcgggataa
    20281 aggcaatgtg gatggggatg atgttctgac tcatcatgcc catcacaagg atgatggaaa
    20341 gcagcagcca tttgaccgcg ccctcgcccg aacgcacgct gtcgggcata ccgccccggt
    20401 tcagtttgcg gacgaccgcg ccggcaagct gctgcggcag gccggaatgg gtaatcgcca
    20461 tcgcaaacgc accgagcatc gcataagaaa gcgcaatctt cgcgccgcct tccaaacctt
    20521 tgttgaacac ggggataatc cccgcctgac tgacctgtcc cgccgcatcg gcaatgtttt
    20581 gcagcggcat acccgccacc gcgccgccga caaacgcgcc gaccgtcagg ctcaatacca
    20641 cgtgcacgcg cgacagcgac agcaccagca taacgattac ggcaactacg actgcattca
    20701 ttgtttatcg ctccaaacct ataaatgttt acatatcgaa acacatcata acccaataac
    20761 gggaaacccg ccaattttgc aaacaattat ttcaaatgct tcatatactt ccccagcgta
    20821 accctgtcca aacccgccaa atccggcagg gtttccactc ctgaaaaacc attctccgcc
    20881 aacacgccgc gcaccgccgc gccctgatcg aaaccgtgtt ccagcaataa aaaaccgcct
    20941 tccgccaaac ggtcgggcgc gccttgcgcc aaggtacgga tgcagcttag gccgtctgaa
    21001 aagtcggtca gcgcgatttg cggctcaaac cgcaaatcgc cttgcgacaa atgtttatcg
    21061 ccgttttcga tatagggcgg gttggacacg atgatgtccc atttcccttc agacggcata
    21121 tcggtgtcga accacgaacc gtgtgcaaat tcgacccgcg cgcccaaatc cgccgcattt
    21181 ttccgcgccg tttcaagggc gggcgggctg atgtcggatg cgcgcacaaa cgcatcgggg
    21241 cgttcgagtg cgacggttac ggcaaccgcg ccgctgcccg tccccaaatc ccacacgcgc
    21301 ccgttttcgg gcaggcgcgc caatacggct tcgaccaagt gttcggtttc ggggcgcgga
    21361 atcagcacgc tcggattgac tgtaaagcgt ctgccataaa attcgcgcac acctaaaata
    21421 taggcaaccg gctcgccgtt cagacggcgt tgcgccagcc tgtccgcccg ctgtcggact
    21481 tcgtccggca tttcttcccc gccccgcgtc aacaactgca cgcgcgtata ttccgaaaca
    21541 tattgcagca gcattcttgc ttcaatttta ggcagttttg acaagcccaa ccattcgtca
    21601 aacgtcattt tttatcccgt ctgccgccga tgcggctttt ctttccttat tctttccggc
    21661 aaacgtaccg atggtggcaa ccgcaaatgc ggcataccac aaataaaatc ctgcaccgta
    21721 gcgcacaata tccgatgtat tccctgcttc atcgacgtat acggctttca cactgaaagc
    21781 caccaacgcc aagccccaaa gtgccgcatg gacaggcacg accttcttcc gcaacgccag
    21841 caaaacaatg gccgccaacc aaacataatt cgcatagacc gcacaatacc tgatatccaa
    21901 agaagcaaat atcgacccca aaatcaaaac ggtcaaaccc tccatgcttc catggttgcc
    21961 caaataaaat gcaacattgg ataaagacgc tatccacagg gcaaccgaca ccagcaacat
    22021 cactatggga aaacttggtt tccgattctg ttcctgcatg gttttatcct aatgtaaaag
    22081 gccgcctgaa aacctttcag acggcatcgt gccggattcc gcgtcagatt gcgctgccgc
    22141 cgacggtcag tccggcatca atccgcaaag tcggttgccc cacgccgacg gggacgctct
    22201 gcccttcttt gccgcacacg ccgacaccgc tgtcgagcgc ggtatcgttg cctatcatcg
    22261 aaacgtgttt cagcacttcg ggaccgttgc cgataatggt cgcgcctttg acgggatatt
    22321 gcagcctgcc gccttccacc caccatgctt cggacgcgct gaacacaaat ttgccgctgg
    22381 taatgtccac ctgtccgccg ccgaagttga cggcgtaaat gcctttgtcg atggacgcga
    22441 tgatttcttc cggctcatag ctgccgtttt ccataaaggt attggtcatg cgcggcatag
    22501 gggcggaggc gtagctttcg cggcggccgt tgccggtcga ttgcgtaccc gtcaggcggg
    22561 cgttggtttc gtcctgcata tagccggtca aaataccgtc ttcaatcaat acggtgcggc
    22621 gggtttcgtt gccttcgtcg tcgatgttga gcgaaccgcg ccggccggca atatcgccct
    22681 gatcgacgac ggtaacgcct ttggcggcga cgcgctcgcc tattctgccg gaaaagacgc
    22741 tggtcccctt gcggttgaaa tcgccttcca aaccgtgtcc gaccgcttcg tgcagcaaca
    22801 cgcccggcca gccgttgccc aaaacgacgg tcatttcgcc ggcgggcgcg gggcgggatt
    22861 cgaggttggt gagtgcctgt ttgacggcgg catcgacaaa ccgatgaacc aagttttcat
    22921 cgaaataagc caagtcgtag cgtccgccgc cgcccgcgct gccctgttcg cggcgttcgc
    22981 cctgtttggc gataacggta acgttcaggc gcaccatcgg gcggatgtcg gcggcgtgtt
    23041 tgccgtccag acgggcgagg taaaccatat cgtattcgca ggtcaaaccg gccatcactt
    23101 gcacgatgcg cggatcggcg gctttggcga ttgcttccac tttgttcaac agcgcgactt
    23161 tggcggcgga atcgaggccg gcaatggggt cggacgcgga acaaaccggc ttgccgcgcg
    23221 tttcagacgg cattttggcg gacaccttgc cgcctgccgc cccaatcgcg cggacggcgc
    23281 gggcggaacg gtttatcgaa tcgatgcaca ggctgtcggc gtaggcaaag gcggttttgt
    23341 cgcccgaaac ggcgcgcacg cccacgccct gattgatttg gaagctgccc gatttgacga
    23401 tgccctcttc caaatgccag ctttcataag cggtgcgctg gcagtagatg tcggcgtaat
    23461 cgacgtggtg cgcgccgatg atgcacaggc ttttggcgag cagttcgggg gaaaggcggt
    23521 tggcttcgag cagccgcgcc tgtacggcgg aataggtcgg atgcatagtg tcggcgcata
    23581 aaaaatcagg ggcttgatta tacggcattt gttatatagt ggattaacaa aaa
//
LOCUS       DAXHRI010000015        23613 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXHRI010000015 DAXHRI010000000
VERSION     DAXHRI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.015"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            307..951
                     /gene="rplC"
                     /locus_tag="ACFM75_000438"
     CDS             307..951
                     /gene="rplC"
                     /locus_tag="ACFM75_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC6255031.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            951..1571
                     /gene="rplD"
                     /locus_tag="ACFM75_000439"
     CDS             951..1571
                     /gene="rplD"
                     /locus_tag="ACFM75_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC6255032.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1568..1882
                     /gene="rplW"
                     /locus_tag="ACFM75_000440"
     CDS             1568..1882
                     /gene="rplW"
                     /locus_tag="ACFM75_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC6255033.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1888..2721
                     /gene="rplB"
                     /locus_tag="ACFM75_000441"
     CDS             1888..2721
                     /gene="rplB"
                     /locus_tag="ACFM75_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC6255034.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2728..3006
                     /gene="rpsS"
                     /locus_tag="ACFM75_000442"
     CDS             2728..3006
                     /gene="rpsS"
                     /locus_tag="ACFM75_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC6255035.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3015..3344
                     /gene="rplV"
                     /locus_tag="ACFM75_000443"
     CDS             3015..3344
                     /gene="rplV"
                     /locus_tag="ACFM75_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC6255036.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3354..4046
                     /gene="rpsC"
                     /locus_tag="ACFM75_000444"
     CDS             3354..4046
                     /gene="rpsC"
                     /locus_tag="ACFM75_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC6255037.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4030..4446
                     /gene="rplP"
                     /locus_tag="ACFM75_000445"
     CDS             4030..4446
                     /gene="rplP"
                     /locus_tag="ACFM75_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC6255038.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4446..4637
                     /gene="rpmC"
                     /locus_tag="ACFM75_000446"
     CDS             4446..4637
                     /gene="rpmC"
                     /locus_tag="ACFM75_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC6255039.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4637..4900
                     /gene="rpsQ"
                     /locus_tag="ACFM75_000447"
     CDS             4637..4900
                     /gene="rpsQ"
                     /locus_tag="ACFM75_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC6255040.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5122..5490
                     /gene="rplN"
                     /locus_tag="ACFM75_000448"
     CDS             5122..5490
                     /gene="rplN"
                     /locus_tag="ACFM75_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC6255041.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5502..5825
                     /gene="rplX"
                     /locus_tag="ACFM75_000449"
     CDS             5502..5825
                     /gene="rplX"
                     /locus_tag="ACFM75_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC6255042.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5835..6374
                     /gene="rplE"
                     /locus_tag="ACFM75_000450"
     CDS             5835..6374
                     /gene="rplE"
                     /locus_tag="ACFM75_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC6255043.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6377..6682
                     /gene="rpsN"
                     /locus_tag="ACFM75_000451"
     CDS             6377..6682
                     /gene="rpsN"
                     /locus_tag="ACFM75_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC6255044.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6698..7090
                     /gene="rpsH"
                     /locus_tag="ACFM75_000452"
     CDS             6698..7090
                     /gene="rpsH"
                     /locus_tag="ACFM75_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC6255045.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7104..7637
                     /gene="rplF"
                     /locus_tag="ACFM75_000453"
     CDS             7104..7637
                     /gene="rplF"
                     /locus_tag="ACFM75_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC6255046.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7651..8004
                     /gene="rplR"
                     /locus_tag="ACFM75_000454"
     CDS             7651..8004
                     /gene="rplR"
                     /locus_tag="ACFM75_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC6255047.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8023..8541
                     /gene="rpsE"
                     /locus_tag="ACFM75_000455"
     CDS             8023..8541
                     /gene="rpsE"
                     /locus_tag="ACFM75_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC6255048.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8534..8719
                     /gene="rpmD"
                     /locus_tag="ACFM75_000456"
     CDS             8534..8719
                     /gene="rpmD"
                     /locus_tag="ACFM75_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC6255049.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8721..9155
                     /gene="rplO"
                     /locus_tag="ACFM75_000457"
     CDS             8721..9155
                     /gene="rplO"
                     /locus_tag="ACFM75_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC6255050.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9167..10477
                     /gene="secY"
                     /locus_tag="ACFM75_000458"
     CDS             9167..10477
                     /gene="secY"
                     /locus_tag="ACFM75_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC6255051.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10482..10700
                     /gene="infA"
                     /locus_tag="ACFM75_000459"
     CDS             10482..10700
                     /gene="infA"
                     /locus_tag="ACFM75_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC6255052.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10721..10834
                     /gene="rpmJ"
                     /locus_tag="ACFM75_000460"
     CDS             10721..10834
                     /gene="rpmJ"
                     /locus_tag="ACFM75_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC6255053.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10900..11262
                     /gene="rpsM"
                     /locus_tag="ACFM75_000461"
     CDS             10900..11262
                     /gene="rpsM"
                     /locus_tag="ACFM75_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC6255054.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11282..11677
                     /gene="rpsK"
                     /locus_tag="ACFM75_000462"
     CDS             11282..11677
                     /gene="rpsK"
                     /locus_tag="ACFM75_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC6255055.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11697..12317
                     /gene="rpsD"
                     /locus_tag="ACFM75_000463"
     CDS             11697..12317
                     /gene="rpsD"
                     /locus_tag="ACFM75_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC6255056.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KTRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12343..13329
                     /gene="rpoA"
                     /locus_tag="ACFM75_000464"
     CDS             12343..13329
                     /gene="rpoA"
                     /locus_tag="ACFM75_000464"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC6255057.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13353..13721
                     /gene="rplQ"
                     /locus_tag="ACFM75_000465"
     CDS             13353..13721
                     /gene="rplQ"
                     /locus_tag="ACFM75_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC6255058.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13868..14581
                     /gene="minC"
                     /locus_tag="ACFM75_000466"
     CDS             13868..14581
                     /gene="minC"
                     /locus_tag="ACFM75_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC6255059.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14610..15425
                     /gene="minD"
                     /locus_tag="ACFM75_000467"
     CDS             14610..15425
                     /gene="minD"
                     /locus_tag="ACFM75_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC6255060.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15429..15692
                     /gene="minE"
                     /locus_tag="ACFM75_000468"
     CDS             15429..15692
                     /gene="minE"
                     /locus_tag="ACFM75_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC6255061.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15696..16616
                     /gene="oxyR"
                     /locus_tag="ACFM75_000469"
     CDS             15696..16616
                     /gene="oxyR"
                     /locus_tag="ACFM75_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC6255062.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16613..16804)
                     /locus_tag="ACFM75_000470"
     CDS             complement(16613..16804)
                     /locus_tag="ACFM75_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255063.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(16946..19783)
                     /locus_tag="ACFM75_000471"
     CDS             complement(16946..19783)
                     /locus_tag="ACFM75_000471"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC6255064.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLF
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETEELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPGMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLAHFPEADGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(19880..20689)
                     /gene="zupT"
                     /locus_tag="ACFM75_000472"
     CDS             complement(19880..20689)
                     /gene="zupT"
                     /locus_tag="ACFM75_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC6255065.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(20870..22126)
                     /locus_tag="ACFM75_000473"
     CDS             complement(20870..22126)
                     /locus_tag="ACFM75_000473"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC6255066.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTASRYQ
                     INKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQNISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPKGGDLNQTLFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSGDLSLSRYQA"
     gene            complement(22205..23596)
                     /locus_tag="ACFM75_000474"
     CDS             complement(22205..23596)
                     /locus_tag="ACFM75_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6255067.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPLMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFMPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
BASE COUNT         6138 a         4979 c         6019 g         6477 t
ORIGIN      
        1 cagcatacga aaactaccat ctcaaagaaa taatggatgc agtcatcaac catattccgg
       61 tttgtcggag aaagatgcat acgctgtgat gaccggatac cgacccttta aaagagtccg
      121 gccctatgcc gtctgaagat tcaaaacgct tcagacggca tattgaagat atttctgata
      181 tttctgttga tatttctttg acttgtcaga tataatgccg agcttggtac atttgtgcca
      241 agtttaactt tgtctgaaag acaggccaat cgtagcctgt ccctttactt taaaaggaaa
      301 ataatcatga ctttaggtct ggttggacgc aaagttggta tgacccgcgt gttcgacgaa
      361 cagggtgttt ctgttccggt aaccgttttg gatatgtctg ccaaccgcgt tacacaagta
      421 aaatccaaag atactgacgg ctatactgcc gttcaagtta cctttggtca gaaaaaagcc
      481 aatcgtgtca ataaagccga agccgggcac tttgcaaaag caggtgttga agccggtcgc
      541 ggtttgattg agtttgcttt gactgaagaa aaactggctg aattgaaagc tggtgacgaa
      601 atcaccgttt ctatgtttga agtcggtcaa ctggtcgatg taaccggtac ctctaaaggt
      661 aaaggtttct ccggcacgat taaacgtcat aacttcggtg cccaacgtac ttcccacggt
      721 aactctcgtt ctcaccgcgt tccaggctct atcggtatgg cgcaagaccc gggtcgcgtg
      781 ttccccggta agcgcatggc cggccaatac ggcaacacca aagcaactgt tcaaaaattg
      841 gaagttgtcc gtgttgatgc agaacgccaa ctgctgttgg ttaagggtgc tgttccgggt
      901 gcggtcaaca gcgatgttgt agttcgtccc agcgtgaaag taggtgcgta atggaattga
      961 aagtaattga cgctaaagga caagtttcag gcagcctgtc tgtttctgat gctttgttcg
     1021 cccgcgaata caatgaagcg ttggttcatc agctggtaaa tgcctacttg gcaaacgccc
     1081 gctctggtaa ccgtgctcaa aaaacccgtg ccgaagtaaa acactcaacc aaaaaaccat
     1141 ggcgtcaaaa aggtaccggc cgcgcccgtt ccggtatgac ttcatctccg ctgtggcgta
     1201 aaggtggtcg cgcgttcccg aacaaacccg acgaaaactt cactcaaaaa gtaaaccgca
     1261 aaatgtaccg tgccggtatg gcgactattc tgtcccaatt gactcgtgac gagcgtttgt
     1321 ttgcgattga ggcgttgact gctgaaactc ctaaaaccaa agtttttgct gaacaagtga
     1381 aaaatctggg tctggagcaa gtgttgtttg taaccaaaca gctcgacgag aatgtttact
     1441 tggcttcacg caacttgcca aacgtgttgg ttttggaagc tcaacaagtt gatccttaca
     1501 gcttgctgcg ttacaaaaaa gtaatcatca ctaaagatgc agttgcacaa ttagaggagc
     1561 aatgggtatg aatcaacaac gtttgactca agtgattttg gcacctatcg tttctgaaaa
     1621 aagcaacgta ttggctgaaa aacgcaacca aatgacgttt aaagtcttgg caaatgcaac
     1681 caaacctgaa atcaaagcgg ctgttgagct gctgtttggt gttcaagttg cttctgtaac
     1741 taccgttaca actaaaggca aaactaagcg ttttggtcgt actttgggcc gccgcagcga
     1801 tgttaaaaaa gcttatgtaa gcttggctgc cggtcaagag ttggatttgg aagccgctgc
     1861 tgcagctgca gataaggaat aaacaaaatg gcaatcgtta aaatgaagcc gacctctgca
     1921 ggccgtcgcg gcatggttcg cgtggtaaca gaaggtttgc acaaaggtgc accttatgcg
     1981 cccttgcttg aaaagaaaaa ttctactgcc ggccgtaaca ataatggtca tatcaccacc
     2041 cgtcacaaag gcggcggtca taaacaccat taccgtgttg tagactttaa acgtaacaaa
     2101 gacggcattc ctgccaaagt agagcgtatc gaatacgatc ctaaccgtac tgccttcatc
     2161 gcactgttgt gctatgcaga cggcgagcgt cgttacatca ttgctcctcg cggtattcaa
     2221 gccggtgctg tattggtttc cggtgctgaa gctgccatca aagtaggcaa caccctgccg
     2281 attcgcaaca ttcccgttgg tacgactatc cactgtatcg aaatgaaacc cggtaaaggt
     2341 gctcaaatcg cacgttctgc cggtgcttct gcggtattgc tggctaaaga aggcgcgtac
     2401 gctcaagtcc gtctgcgctc tggcgaagtc cgtaaaatca acgtaaattg ccgtgcgacc
     2461 attggtgaag ttggtaacga agagcaaagc ctgaaaaaaa tcggtaaagc cggtgccaac
     2521 cgttggcgcg gtattcgtcc gaccgtacgc ggtgttgtca tgaatcctgt cgatcacccg
     2581 catggtggtg gtgaaggccg tacgggcgag gcccgcgaac cggtcagccc atggggtact
     2641 cctgctaaag gctaccgcac tcgtaataac aaacgcacgg ataacatgat tgttcgtcgc
     2701 cgttactcaa ataaaggtta atttagtatg gctcgttcat tgaaaaaagg cccatatgta
     2761 gacctgcatt tgctgaaaaa agtagatgct gctcgcgcaa gcaacgacaa acgcccgatt
     2821 aaaacctggt ctcgtcgttc taccattctg cctgatttta tcggtctgac cattgctgtg
     2881 cacaacggcc gcacccatgt gcctgtgttt atcagcgaca atatggttgg tcataaatta
     2941 ggcgaattct cattgacccg tacctttaaa ggccacttgg ccgataaaaa ggctaaaaag
     3001 aaataaggtg aatcatgaga gtaaatgcac aacataaaaa tgcccgtatc tctgctcaaa
     3061 aggctcgttt ggtagctgat ttgattcgtg gtaaagacgt tgcccaagct ttgaatattt
     3121 tggctttcag tcctaaaaaa ggtgccgagc tgattaaaaa agtattggag tcagctattg
     3181 ctaatgccga gcacaataac ggtgcggaca ttgatgaact gaaagtggta actatctttg
     3241 ttgacaaagg cccaagcttg aaacgttttc aagctcgcgc caaaggtcgc ggtaaccgca
     3301 tcgaaaaaca aacttgtcat atcaatgtga cagtgggtaa ctaaggaaaa gctatgggac
     3361 aaaagattaa ccctacaggc tttcgcctgg cggtaactaa agactgggct tcaaaatggt
     3421 ttgctaaaag caccgacttt tctactgttt tgaagcagga tatcgatgtt cgcaattatt
     3481 tgcgtcaaaa attggccaat gcttcggttg gtcgagtggt tattgaacgc cctgcaaaat
     3541 ctgcacgcat taccattcac tccgctcgtc cgggtgtggt tatcggtaaa aaaggtgagg
     3601 atatcgaggt tttgaaacgt gacttgcaag tcttgatggg tgtacctgtt catgtaaata
     3661 ttgaagagat tcgccgtcct gagttggatg ctcaaattat tgctgacggt attgcccagc
     3721 agttggaaaa gcgcgttcaa ttccgtcgtg ctatgaaacg agcaatgcaa aatgcaatgc
     3781 gttctggtgc taaaggcatt aagattatga cttcaggccg tctgaatggt gcggatattg
     3841 cccgtagcga atggtatcgt gaaggtcgcg tgccactgca tactttacgt gcaaatgtag
     3901 attatgcaac cagcgaagcg cacaccacat atggtgtatt gggtctgaaa gtttgggttt
     3961 atacggaagg caatattaaa tcttccaaac ctgaacatga gagtaaacaa agaaaggcag
     4021 gtagacgtaa tgctgcagcc aactagactg aaataccgta agcaacaaaa gggtcgcaat
     4081 actggcatcg ctactcgcgg taataaggta agtttcggtg agttcggctt gaaagccgta
     4141 ggtcgtggtc gtttgactgc ccgtcaaatc gaagctgctc gtcgtgcaat gacccgtcat
     4201 atcaaacgtg gtggtcgtat ttggattcgt gtattccctg ataaaccgat tactgaaaag
     4261 cctattcaag ttcgtatggg tggtggtaaa ggtaacgtgg aatattacat tgccgaaatt
     4321 aaaccaggta aagtgttgta tgaaatggat ggcgttccag aggaactggc tcgtgaagca
     4381 ttcgagttgg ctgctgccaa attgcctatt cctacaacct ttgtagtaag acaggtgggt
     4441 caataatgaa agcaaatgaa ttgaaagaca aatccgttga gcagttgaat gcagatttgt
     4501 tggacttgtt gaaagctcag tttggcttac gtatgcaaaa cgctaccggt caattaggca
     4561 aaccaagtga attgaaacgt gtacgtcgcg atattgctcg tattaaaacc gttttaactg
     4621 aaaaaggtgc taagtaatga gcgaaactaa aaatgttcgt actttgcaag gcaaagtagt
     4681 aagcgacaaa atggataaaa ccgtaacagt attggttgag cgtaaagtaa aacatccgct
     4741 gtatggtaag attattcggt tatctactaa aatccatgcc catgatgaaa ataatcaata
     4801 tggaattggt gatgtggttg ttatatcgga atcccgtcca ttgtcaaaaa ctaaatcttg
     4861 ggttgtcagt gagctggttg agaaagcacg ttctatttaa gaattaaagc aacgtgcttg
     4921 gaatgggaaa cgaagtattg cagcaaattt aatttgcgtg taaacttcgt ttcctgtctt
     4981 tcagtttctt ctggaagttt cttccctttc ggggtccaag actggtttac ttgaaccgca
     5041 aggtttcatt taataagcag cggctttgct gtaagttatc tgaaagtggt aaattaagtt
     5101 ggttaattta aaggtaataa catgattcaa atgcagacca tcttagatgt ggctgataac
     5161 tctggtgcgc gtcgcgtaat gtgtatcaag gtattgggcg gatctaagcg tcgctacgct
     5221 tctgttggcg atattattaa agtggcagtt aaagatgcgg ctccgcgtgg ccgtgtcaaa
     5281 aaaggcgatg tatataatgc ggtagttgtt cgtactgcta agggtgtacg tcgtcctgat
     5341 ggtgcgttaa ttaaattcga taacaatgcc gccgtgttac tgaataataa acttgaacct
     5401 ttgggtactc gtatctttgg tccggtaacc cgtgaattgc gtactgagcg atttatgaaa
     5461 atcgtttcat tggcacctga agtattataa ggaatggcac gatgaataaa atcattaaag
     5521 gcgatagggt tgtagtaatt gctggtaagg ataaaggtaa gcagggtcaa gtagttcgag
     5581 tgttgggtga taaagttgtt gttgagggcg ttaatgttgt aaaacgccat caaaaaccta
     5641 atccaatgcg tggcattgag ggcggtatta ttactaaaga aatgcctttg gatatttcta
     5701 atatcgcaat cctgaatccg gaaactaata aagcggaccg tgttggtatt aagctgattg
     5761 aaaatgaagg caaagttaaa cgcgttcgtt tcttcaaatc aaatggctct atcattgggg
     5821 cataaggaga taacatggct cggttgagag agttttataa agagacagtt gttcctgaat
     5881 tggttaaaca atttggttac aaatcagtaa tggaagtccc gcgtattgaa aaaattacct
     5941 tgaatatggg tgtgggtgag gctgttgctg ataaaaaagt tatggaacat gctgtttccg
     6001 atttagagaa aattgccggt caaaaaccgg ttgttactgt tgcccgtaaa tctatcgcag
     6061 gttttaaaat ccgtgataac tatccggttg gttgcaaagt aacattgcgt cgtgatcaaa
     6121 tgtttgaatt cttggatcgt ttgattacta ttgcattacc tcgcgtacgt gacttccgtg
     6181 gtgtgagcgg taaatcattt gatggccgtg gcaattacaa tatgggtgtt cgtgagcaaa
     6241 ttatttttcc ggaaattgaa tacgataaaa ttgatgcttt gcgtggtttg aatattacta
     6301 ttactactac agcaaaaacc gatgaggaag cgaaagcttt attgtcattg tttaaatttc
     6361 cgttcaaagg ataatcatgg ctaagaaagc acttattaat cgtgatctga aacgtcaagc
     6421 tttggctaaa aaatatgcgg ctaaacgcgc ggcaattaaa gcggtaatca atgattcgaa
     6481 tgcaactgag gaagagcgtt ttgaggctcg tttgaggttt caatccattc ctcgtaatgc
     6541 ggcacctgtg cgtcaacgtc gtcgttgtgc tttgacaggt cgccctcgtg gtactttccg
     6601 taaatttggt ttgggtcgta ttaaaatccg tgaaatcgcc atgcgtggcg aaattccggg
     6661 tgttgttaaa gccagctggt aataggagta attaagaatg agtatgcatg atcctatttc
     6721 cgatatgttg actcgtatcc gcaatgcgca acgtgctaat aaagcagcag ttgcaatgcc
     6781 ttcttcaaaa ttaaagtgtg ctattgcaaa ggtattgaaa gaagaaggat atattgagga
     6841 cttcgcagtt tcatctgacg taaagtctat attggaaatt caattaaaat actatgcagg
     6901 tcgtcctgta attgaacaaa tcaagcgtgt atctcgccct ggtttgcgta tttataaagc
     6961 gtctagtgag attccaagtg ttatgaatgg cttgggtatt gctattgtta gtacttctaa
     7021 aggtgtaatg actgatcgta aagcacgttc tcaaggtgtt ggtggtgagt tgttatgcat
     7081 tgtagcctag tggaggaaaa gaaatgtcac gtgtcgcaaa aaacccagtg actgttcccg
     7141 ctggtgtaga agtaaaattt ggaacagagg cattagttat taagggtaag aacggtgaat
     7201 tgtcttttcc tttgcattct gatgtagcca ttgaatttaa tgatggcaaa ttggcttttg
     7261 ttgcgaataa cagcagtaaa caagcaaatg caatgtctgg tactgctcgc gcattagtca
     7321 gcaatatggt taaaggtgtt tcagaaggtt ttgagaaaag attgcaattg ataggtgtgg
     7381 gttatcgtgc tcaagcacaa ggtaaaatct tgaatctgtc tttgggtttt tctcatccga
     7441 tcgtatatga aatgcctgaa ggtgtctccg ttcaaactcc tagccaaaca gagattgttt
     7501 taaccggctc ggataaacaa gttgttggtc aagttgctgc tgagattcgt gcgttccgtg
     7561 ctcctgagcc ttataaaggt aaaggtgttc gctatgtagg agaagtagtg gtaatgaaag
     7621 aagccaagaa aaaataattg aggttcacta atggataaac atacaacccg actccgtcgt
     7681 gcacgcaaaa cccgtgctcg tattgcggac ttgaaaatgg taagattatg tgtgttccga
     7741 agcaataatc atatttatgc tcaagtaatt agtgctgaag gtgataaagt attggctcaa
     7801 gcctctacat tggaagctga ggtgcgcggt agtctgaaat ctggaagcaa tgttgaagca
     7861 gctgcaatag ttggtaaacg tattgctgag aaggctaaag cagcaggtgt agaaaaggtt
     7921 gcttttgatc gttcaggttt ccaatatcac ggtcgtgtga aggctttggc tgaagctgct
     7981 cgtgaaaatg gtttaagctt ctaaatattt ggagactttc agatggcaaa acatgaaatt
     8041 gaagaacgcg gtgacggtct gattgaaaag atggtcgcag ttaaccgtgt aaccaaagta
     8101 gttaaaggtg gtcgcattat ggctttctct gcgctaactg ttgttggtga tggagatggt
     8161 cgtattggta tgggtaaagg taagtcaaaa gaagtgcctg ttgcagtcca aaaggcgatg
     8221 gatcaagcac gacgctctat gattaaggta ccattaaaaa atggtacgat tcatcatgag
     8281 gttattggtc gacatggtgc tactaaagta tttatgcagc ctgctaaaga gggtagtggc
     8341 gtaaaagccg gtggacctat gcgtttggtt tttgatgcta tgggcattca taatatctcc
     8401 gccaaagtgc acggatctac taacccatat aatatcgtac gtgcaacatt agatggtttg
     8461 tctaagttgc atactcctgc tgatatcgca gccaaacgtg gcttgacagt ggaagacatt
     8521 ttgggagtta accatggctg aacaaaaaaa gattagggtt acattggtta aaagcctgat
     8581 tggtacaatt gaatctcatc gtgcatgtgc acgcggttta ggtttgcgtc gtcgcgagca
     8641 tacggtagag gttttagata cccctgaaaa ccgtggtatg attaataaaa tcagctactt
     8701 gttgaaagtg gagtcttgat atgtttttga atacaattca acctgctgtt ggtgctacgc
     8761 atgctggtcg tcgtgttgga cgcggtattg gtagtggtct tggcaaaacg ggtggtcgtg
     8821 gtcataaagg tcaaaagagc cggtctggtg ggtttcataa ggtgggtttc gagggtggtc
     8881 aaatgccctt gcaacgacgc ctccctaaaa gaggttttaa atctttaaca gcatcagcta
     8941 atgcacagct tcgtttaagt gaactggaat caattgctgt taatgagatt gatattttgg
     9001 tcttaaagca agcgggtctg attgcatcta cagtctctaa tgttaaagtt attgcttctg
     9061 gtgaaatttc taaggcagtt gctttgaagg gtattaaagt taccaaaggt gcgagagctg
     9121 ctatcgaggc tgttggtggt aagattgaaa tgtaaggttt aatattgtgg ctaatcaaca
     9181 aacgtcatca ggttcatcca aatttggaga tcttaagaaa cgtcttttgt ttctatttgg
     9241 agcattgatt gtttttcgaa ttggtgccca tatacccgta cctggagttg atgctgttgc
     9301 tttagctaaa ttatacgaaa gcgctggaaa cggcatcctg ggaatattga atatgttttc
     9361 cggtgggtcg ttagagcgct ttagtatatt tgcaatagga attatgccat atatttcagc
     9421 ttctattatt gtacagctcg cttctgaaat tttgccatca ttgaaggctt taaaaaaaga
     9481 aggggaggct ggtagaaagg taattacgaa atatactagg tatggtactg ttttgttagc
     9541 aattcttcaa agtctaggtg ttgcatcttt cgtatttcag caaggaattg ttgtaacaag
     9601 ttcatttgag tttcatgttt ccacggtagt ttctttggta acgggaacta tgtttcttat
     9661 gtggcttggg gagcaaatta ctgaaagggg tatcgggaac ggtatttctt taatcattac
     9721 ggcaggtatt gcttcaggta ttccttcggg tattgcaaag ctggttacac tgacgaacca
     9781 aggttctatg agcatgctta cggcgttgtt tattgtattt ggtgccttat tattaattta
     9841 tttggttgta tactttgaaa gtgcacagcg gaagattcct attcattatg caaaacgcca
     9901 gtttaatggt agggcgggta gtcaaaatac gcatatgcct ttcaagttga atatggctgg
     9961 tgttattccc ccaatttttg cttccagtat tattctattt ccatctactc ttttaggttg
    10021 gtttggttcg gctgatacaa atagtgtttt gcacaaaata gctggattgt tacaacacgg
    10081 tcaattgctg tatatggctt tatttgcagc gacagttatt ttcttttgtt atttttatac
    10141 ggctttggtt tttagcccta aagaaatggc agagaattta aaaaagagtg gtgcttttgt
    10201 tcctgggatt agacctggtg agcagacctc taggtattta gaaaaagttg tattacgttt
    10261 gacattgttt ggagctcttt atattacaac tatttgttta attccagagt tcttaactac
    10321 ggttttaaat gtaccttttt atttgggtgg cacgtctttg ttgattctag ttgttgtaac
    10381 gatggatttt agtacacaaa taaattcgta taggcttact caacagtatg ataagttaat
    10441 gactcgttca gaaatgaaat cattttctcg gaaatagaat tatggcgaaa gaagatacta
    10501 tccaaatgca aggtgaaatt cttgaaactt tacctaatgc aacatttaaa gtaaaacttg
    10561 agaatgacca tattgtattg ggtcatattt ctgggaagat gcggatgcat tacattcgta
    10621 tttctccggg agataaggtc acagtagagc tgacacctta tgatctaact agggctcgaa
    10681 tcgttttcag agcaagataa accaataaaa ggaaaataaa atgcgtgtac aaccatctgt
    10741 taagaaaatt tgccgaaatt gcaagattat tcgtcgaaat cgtgtagttc gtgtaatttg
    10801 tactgatctc cgtcacaaac agcgtcaagg ttaatggaat atttctttta atgtgattct
    10861 gtgatatagt gacacacttt gccctaaaaa ggaaaaaata tggctcgtat tgcaggggta
    10921 aatatcccta ataacgcaca catcgtaatt ggtcttcagg ctatttacgg tattggtgct
    10981 actcgtgcta aattgatttg tgaggctgca aatattgcgc ctgatactaa agcaaaagat
    11041 ttggacgaga ctcaattaga tgctttgcgt gaccaagttg ccaagtatga agtagaaggt
    11101 gatttgcgtc gtgaggtaac tatgagtatc aagcgattga tggacatggg ctgctatcgt
    11161 ggcttccgtc atcgtcgcgg cttaccatgc cgcggtcaac gcactcgtac aaatgcgcgt
    11221 acccgcaaag gtccgcgtaa agcgattgct ggtaagaaat aaattttaag gaattttatt
    11281 aatggctaaa gcaaacacag cttcacgtgt acgtaaaaaa gtacgtaaaa ccgtgagtga
    11341 gggtattgtg cacgttcatg catctttcaa caataccatc attacaatca ctgaccgtca
    11401 aggcaatgcg ttgtcttggg ctacctctgg cggcgctggt tttaaaggtt ctcgtaaaag
    11461 tacaccattt gcagcacaag ttgcagcaga agcagctggt aaagttgccc aagagtatgg
    11521 cgttaaaaat ttagaggttc gtattaaagg tccaggtcca ggtcgtgaat cctctgtacg
    11581 tgctttgaat gctcttggtt tcaagattac cagcattact gacgttaccc cgttgcctca
    11641 taacggttgc cgtccgccta aaaaacgtcg tatttaatat tggagtgatt tgaaacatgg
    11701 cacgttatat tggccctaaa tgtaagttgg cacgtcgcga aggtacggat ttgtttttga
    11761 agagtgcgcg ccgctctttg gattctaaat gtaaaattga ttccgctccc ggtcagcatg
    11821 gtgcaaaaaa aacgcgtttg tcagactatg gtttgcagtt gcgtgaaaaa caaaaaatcc
    11881 gccgtattta tggcgtatta gaacgtcagt tccgtcgtta tttcgcagaa gctgatcgtc
    11941 gtaaaggttc taccggcgag ttgctgttgc agttgctgga atctcgtttg gataatgtcg
    12001 tttatcgtat gggtttcggt tctacccgag ctgaagcaag acagcttgtt tctcataagg
    12061 cgatagttgt gaatggacaa gttgtcaata ttccttcttt ccaagtgaaa gctggtgatg
    12121 ttgtctcagt tcgtgaaaaa gccaaaaaac aggtacgtat tcaagaagca ttgggtttgg
    12181 caactcaaat cggcttgccg ggttgggttt ctgtagatgc ggataagctt gagggtgtgt
    12241 tcaaaaacat gccggatcgc tcggaattga ccggtgatat taatgaacag ctggtggtag
    12301 agttctactc taaataatgc tagctcagtg agggacagtt aaatgcagaa tagcacaacc
    12361 gaatttttga aacctcgtca aattgatgta aatacttttt ctgcaactcg tgcaaaagta
    12421 tctatgcagc catttgaacg tggtttcggt cataccttag gtaatgcttt gcgccgtatc
    12481 ttactgtcat ccatgaatgg ttttgctcct actgaagtag ctattgccgg tgtattacac
    12541 gaatattcta ctgttgatgg tattcaggaa gatgttgttg acattttgct gaatattaaa
    12601 ggtattgtgt ttaaactcca tggtcgtagc caagttcaac ttgtgttgaa gaaatcaggt
    12661 tcaggtgtcg tatctgccgg tgatattgag ttgccgcatg atgtagaaat tctgaatcct
    12721 ggtcatgtca tttgtcattt ggctgataac ggtcaaattg agatggaaat taaagtagag
    12781 caaggtcgtg gttatcaatc tgtttcaggt cgtcaggtag ttcgtgatga gaaccgtcag
    12841 attggtgcaa tccagttgga tgcgagcttt tcgcccatca gccgtgttag ctttgaggtt
    12901 gaacctgcac gtgtagagca gcggacggat cttgataagt tggttttgga tatcgaaacc
    12961 gacggttcta ttgatcctga ggaagctgta cgcagtgcgg cacgtatttt gattgatcag
    13021 atgtctattt ttgctgattt gcagggtacg cctgtggagg aggttgaaga aaaagcacct
    13081 cctatcgacc ctgttctttt gcgtccggtg gatgatctgg aattgacagt acgttcagct
    13141 aattgtttga aagctgagga tatttattat attggcgatt tgattcaacg cactgaaacc
    13201 gagcttctta aaacgccgaa tttgggacgt aaatctttga atgagattaa ggaagtattg
    13261 gcatctaaag gtttgacact gggttctaag ttggaagcat ggccacctgt aggcttggaa
    13321 aagccttaat gaagaattaa aggataattg atatgcgtca tcgtaatggc aatcgcaaat
    13381 taaaccgtac cagcagtcat cgtgctgcaa tgctgcgtaa tatggcgaat tcattattga
    13441 ctcacgaaac tattgtaaca actctgccta aggccaagga attgcgccgt gtagtagagc
    13501 cgttgattac attgggtaaa aagccgtcat tggcaaaccg ccgtttggca tttgaccgta
    13561 ctcgcgaccg tgatgttgta gtaaaactgt ttggcgattt gggtcctcgt tttactgctc
    13621 gtaacggtgg ttatgttcgg gtgttgaaat acggattccg taaaggtgat aatgcacctc
    13681 tggcactggt tgaattggtt gacaaaccgg ctgctgagta attttagtca tataacgcca
    13741 tctgccgaaa agcaggtggc gttatttttg caatatctga taggtaatag ggtattggct
    13801 atcatgttta aaatattaat tgaatagcta aggtttgcgc ggtaaactta catcattaaa
    13861 aaattctatg atggtttata taatgaatgc tttcgatata aagtcgacaa agatggacgt
    13921 attgtctata tctttgcata cgtcagactt gtttgatttg gaagatgtgc tggtcaaatt
    13981 gggcaagaag tttcaagagt ctggtgttgt tccatttgtg ctggatgttc aagagtttga
    14041 ttatcccgag tctttggatc ttgctgcatt ggtttcgttg ttttcaaggc atggtatgca
    14101 aattttgggt ctgaagcatt ctaatgaacg ttgggctgct gcggctatga agtatcattt
    14161 gctgttttgt ctgtctcatt cggaaaatgt taaagaactg ggtcaggttg aggtgcagaa
    14221 cacggaggat ggtcagaaag caaggaaaac agtattgatt acatcccctg tccgtaccgg
    14281 tcagcaggtt tatgccgaag atggcgattt gattgttacg ggggcggtca gccagggggc
    14341 ggaattgatt gcggatggca atatacatat ttatgcgccg atgagggggc gtgctttggc
    14401 cggtgccaag ggtgatactt ctgcccgcat atttatccac tccatgcagg cagaactggt
    14461 ttctgtggcg ggtatttacc gtaattttga acaggatttg ccgaaccatc tgcacaagca
    14521 gccggtacag atattgttgc aggataaccg attggttatc agtgcaattg gctcagagta
    14581 attgtttgat attttaaaaa ggaaatattg tggcaaaaat tattgtagta acttcaggta
    14641 agggcggtgt cggtaaaacg actaccagtg ccagtattgc gacaggtttg gcattacgcg
    14701 gatataaaac tgcggtaatt gattttgatg tgggtttgcg taacctcgac ctcattatgg
    14761 gttgcgagcg tcgtgtcgtt tatgacctga tcaatgtcat tcagggggag gcgacgctca
    14821 accaggcttt gattaaagat aaaaattgtg aaaacctgtt tattttgccg gcttcccaga
    14881 ctcgggataa agacgctttg acacgcgagg gcgtagaaaa agtgatgcag gagctgtccg
    14941 gcaagaaaat gggctttgag tatattattt gcgactctcc tgccggtatt gagcagggtg
    15001 cattgatggc gttgtatttt gctgatgaag ccattgtaac gaccaatcct gaggtttcca
    15061 gtgtgcgtga ctccgacagg attttgggaa ttttgcaaag caaatcccat aaggcagagc
    15121 aaggcggttc ggttaaagaa catctgttga ttacgcgtta ttctcccgaa cgtgtggcaa
    15181 aaggcgaaat gctgtctgta caggatattt gcgatattct gcatattcct ttgctgggtg
    15241 tgattcctga atcccaaaac gtcttgcagg catccaattc cggagaaccg gtcatccatc
    15301 aggacagcgt ggcggcttcc gaggcatata aggacgttat tgcccgtctt ttgggcgaga
    15361 accgtgaaat gcgtttcttg gaagctgaga aaaaaagctt cttcaaacgt ctgtttggag
    15421 gataaggtat gtcattaatc gaatttttat tcggcagaaa gcagaaaacg gcaaccgttg
    15481 cccgcgaccg ccttcaaatc atcattgccc aagagcgcgc ccaagaaggt caggctccgg
    15541 attacctgcc gactttacgt aaagagttga tggaagtcct gtccaaatat gtgaatgttt
    15601 cattagacaa tatccgtatt tcccaggaaa aacaggatgg tatggatgtg cttgagttga
    15661 acattacttt gccggaacag aaaaaggtat aggacatgac cttaaccgaa ttgcggtaca
    15721 tcgtcgcagt cgcccaagaa cgtcatttcg gcagggcggc gcggcgttgt tttgtcagtc
    15781 agcccacttt gtctattgcc attaagaaat tggaagaaga gcttgccgtc tctttgtttg
    15841 accggagcag caacgatatt attacgaccg aggcggggga acgtatcgtt gcacaggcgc
    15901 gtaaggtgtt ggaagaggca gagcttatca ggcatttggc aaatgaagaa caaaacgagc
    15961 tggagggtgc gttcaaactc gggctgattt ttacggttgc gccgtacctg ctgccgaaac
    16021 tgattgtctc gttgcgccgt actgcaccga aaatgccttt gatgttggaa gagaattaca
    16081 cgcatacttt gaccgagtcg ctcaaacgcg gggacgttga tgcgattatc gttgccgaac
    16141 cgtttcaaga gccgggcatt gttaccgaac ccttgtatga cgaaccgttt ttcgtgattg
    16201 tcccgaaagg gcattcattt gaggaactgg atgccgtttc gccccggatg ctgggtgagg
    16261 agcaggtttt gctgctgacg gaaggcaact gtatgcggga tcaggtactc tcaagctgtt
    16321 ccgaattggc ggcgaaacaa cgtatacagg ggttgaccaa tacattgcag ggcagctcga
    16381 ttaatacaat ccgccatatg gttgccagcg gtttggcaat cagcgtgttg ccggcaaccg
    16441 cactgaccga aaacgatcat atgctgttca gcattattcc gtttgagggt acgccgccaa
    16501 gccggcgggt cgtattggcg taccgccgca attttgtccg tccgaaggcg ttgtcggcga
    16561 tgaaggcggc gattatgcag tcgcagcttc acggggtaag ttttatccgc gactaggcgc
    16621 aggcattgtt ttcaaaacgc catttccctg agccgacaac acggtatgcc aagatattgc
    16681 cgtcatcatc gattttgagt atagcatcgc cacggaaact gccgtcctga agatattcga
    16741 cttttgcatc actgtgaatg ttttcatcag tgccgatgca atgccatgta tagtggatta
    16801 acaaaaacca gtacggcgtt gcctcacctt gccgtactat ttgtactgtc tgcggcttcg
    16861 tcgccttgtc ctgatttttg ttaatccact ataaaagagg ccgtctgaaa aacatttttc
    16921 agacggcctt gtttattcaa tcaaatcagt ctttcaactt cgccaactga ttttgaactt
    16981 ttgccatttt gtcttccaat tccgccaaat cggctttgtc tttttccacc agatgcgcag
    17041 gggctttttc ggtgtagccg ggtttggaga gtttggcgtt gagtttgtcc aaggcttttt
    17101 gcagcttctc ggcttctttg ctcaaacggg cggtttcggc ggctttgtcg atttcgactt
    17161 tcaacatcag gcgcgcgccg ttgcagacgg cgacgggcgc gtctccgctt tcgggcaggg
    17221 cggcgacttg ccgtgcttcg gtcaggcggg tcatcatcgg caggtatttg aggtagtccg
    17281 ccaagtcgtc cgcgctttcg acaaacagcg gggctttgac attaggctgg atgcccattt
    17341 cgccgcgcag gttgcggacg gcgccaatca aatcctgcaa cacggtcatt tgcccgaatg
    17401 ccgtctgaac aatctcgccg ccgtcggctt cggggaagtg ggcgagcata atgctgtcgg
    17461 cggttttcgc gtcgcacata ggggcgacgg tttgccacag ttcttcggtg atgaagggga
    17521 taatcggatg cagcaggcgc agggcggctt cgagtacgcg caacaaggta tggcgtgtgg
    17581 cgcgctggcg gctggcgcag ccggtttgaa gctgcacttt ggcgagttcc aaataccagt
    17641 cgcaatagtc gttccatacg aagctgtaca gggtttccgc cgccaaatca aagcggtagg
    17701 tttcgtaggc ttgcgtaacc tgctcgatgg tctgattcag acggcctaca atccacatat
    17761 cggggaagga atagccgcgc ggttcggcgg cggttgcgcc gtagccgcag tcttggtttt
    17821 cggtgttcat caagacgaag ttggtggcgt tccagatttt gttgcagaag ttgcggtagc
    17881 cttcggcgcg tttgaagtcg aagttgacgc tgcgtccgag gctggcgtag ctcgccatag
    17941 tgaagcgcaa tgcgtccgcg cccataccgg ggatgccttc aggaaagagt tttttggtgg
    18001 cttcttccac tttcggcgcg gtttcgggtt tgcgcaggcc ggtggtacgt tttaccagca
    18061 gtttttccaa gtcgatgccg tcgatcaaat ctacgggatc gatgacattg ccttcggatt
    18121 tggacatttt tttgccttcg tggtcgcgca cgatgccgtg gatgtacacg tctttaaacg
    18181 gtactttgcc ggtaaagtgg gtggtcatca taatcatgcg cgccacccag aagaagatga
    18241 tttcgtagcc ggtaaccaag acgttggacg gcaggaaggc tttgagttct tcggtttcag
    18301 acggccagcc gagcgtggaa aacggcacga gcgcggagga gaaccaagtg tccaatacgt
    18361 cttcttcgcg ggtcaaaccg gttttgccgg cttgtttttc ggcttccgcc tgattgcggg
    18421 cgacgtacac attgcctgct tcgtcgtacc acgcggggat ttgatgcccc caccacagtt
    18481 ggcgcgagat gcaccagtct tggatattgt tcatccattg gttgtaggtg ttgacccagt
    18541 tttcggggat aaagcgtacc gcgccgctgt cgacggcttt tttggctttg tcggcgaggc
    18601 tcaagccttt gaattcgctg tcaggctcgc cgccgttcgg cgtggcggac atagcgacaa
    18661 accattggct ggtcagcatc ggctcaatca ccgaacctgt gcggtcgcct ttcggcgtca
    18721 tcagtgtgtg cggtttgatt tcgaccaaca ggccttgttc ctgcaaatcg gcaaccattt
    18781 gtttgcgcgc ggcaaagcgg tccaaacctg cgtatttttc aggcagggca aagcccggtt
    18841 gcgcttcgcc tttgaagttg aacacttcgg cgtttgccag cactttggct tccaagtcga
    18901 acacattaat caggcgcgtg tcgtggcgtt tgccgacttc gtagtcgttg aagtcgtgcg
    18961 caggcgtgat tttcacgcag cctgtgccga agtctttttc aacgtattcg tcggcaatca
    19021 cgggaatggt gcggccggtc agcggcagga ttaattcttt gccgatcaaa tgggtgtaac
    19081 gttcgtcttc gggattgacg gcaacggcca cgtcgcctag cagcgtctca ggacgggtgg
    19141 tcgccacgat cacggcttcg gcaggattgt ccgccagcgg atagcggatg tgccacatag
    19201 agccttgttc ttccacgctt tccacttcca aatccgatac tgccgtaccc aaaaccggat
    19261 cccagttcac caagcgtttg ccacggtaaa tcaaaccttg ctcaaacagg cgcacgaaca
    19321 cttcggttac ggtttcggcg cgcacgtcgt ccatcgtgaa atactcgcgc gtccagtcgg
    19381 cagagcagcc cacgcggcgc atttgttggg taatcgtgcc gccggacact tctttccatt
    19441 cccacacttt ttccaagaat ttttcgcggc ccaagtcatg acgggacacg ttttgcgcgg
    19501 caagctgacg ctcgaccacg atttgcgtgg cgatgcccgc gtggtcggtg cccggaatcc
    19561 aggcggtgtt gcagcctttc atgcggtagt agcgggtcag accgtccata atggtttggt
    19621 tgaaggcatg gcccatgtgc agcgtgccgg ttacattggg cggcggcagt tggatggaaa
    19681 aagacggttt ggtcaaatcc atatcaggtt ggaaatagcc ttggctttcc cagttttgat
    19741 agtgcttgga ttcgatttcg gcgggggagt atttgtctaa catgatggaa ctttgtgaaa
    19801 ttaaggttat ttttgatgtg cggattataa cgcaaaaagg ccgtctgaat catttcagac
    19861 ggcctttggc atacaggttt taaaaatgga acaataccag gctgacggca atcaccgcca
    19921 tgcccattgt caggccgtaa acggtttcat ggccgtctga atagcgtttg gcagccggca
    19981 gcagctcgtc caacgccaaa aacaccatca caccggctat cacgccgaat accgaaccaa
    20041 acacggcagg cgacaaaaac ggctgcaaaa ccaaatagcc caaagccgcc cccaacggct
    20101 cggccaagcc ggatagcaga cacgcccaca ccgttttctt acggctgcgg gtggcaaaat
    20161 aaaccggcgc ggcgatggaa atgccctccg gaatattatg gatggcaatc gccaaggcca
    20221 aaggcatccc gactgctgga ttttccaatg tggcaaaaaa cgtcgccaag ccttcgggga
    20281 aattgtgcgc agtaatcgca aacgccgcca tcatgccgac tcgcgcgata tggcggcgtt
    20341 tgctttcttg aaacgacggg tcttgcgcgt ctaaagtttc atgcgggttc ggcaccagac
    20401 ggtcaatcag cgcaatgccg cccatcccgg ccaaaaatgc catggtcgcc gccgcaaacg
    20461 cgtggtcttt atcataaatt tcagcgaacg cctcgctgga cttactgaaa atctccgtca
    20521 gggaaacata taccatcgca ccgccggcaa acgccaaacc aaacgacaac acacgcggat
    20581 tgggcgtttt ggaaaacatc accaagccac tgcctaatac ggtaaacaaa ccggcagcca
    20641 atgtgatgga aaaggcaacg gccaaattgg acatcgaaaa atcgggcatg agaaaacctg
    20701 cgctaaaagc tgggacaggt tcagactaac actttttaat gtatatgata atagttatta
    20761 tttattttat tgattggata cacggatttt gaaacaaaag gccgtctgaa aaatgatttt
    20821 cagacggcct ttaaatttga aatgccgcta aaccttagtg ctttccagct taagcctgat
    20881 aacgcgacag gctcaaatcg ccgcttcgga tttcggtgtc tttgccgctt acgatatcgg
    20941 cggttaattt tgccgaaccc agcgacatgg tccagcccaa agtgccgtgg ccggtattca
    21001 ggaacaggtt ttcaaagcgg gtgcgcccga ttaacggcgt gctgtcgggc gtcatcggtc
    21061 tgaggccgct ccagaacaat gtttggttta aatcgccgcc ttttgggaac aagtcgttga
    21121 cgaccaaagc caaggtttcg cggcgttttt cgggcagttt gatttcgtag cccgacaatt
    21181 ccgccatacc gccgacgcgg attctgttgt caaagcgcgt gatggcgact ttgtagcttt
    21241 catctaaaac ggtggacacc ggtgcgccgt ctgaattggt gaccggcagg gtcaaggaat
    21301 agcctttgac gggataaatg ggcagattga gatccaactg cgccaaaacc gtcctgctga
    21361 aacaaccgag cgcgcagaca acggcatctg cttcaaaccg ccctgtttcg gtttcaacgg
    21421 ttttgatgcg cagcccgttg tggtcgatgc ggctgatgtt ttggttgaaa tgaaaccgta
    21481 cgcccttttc ctgacacaat ttgtataggt tttcagtgaa gaggcggcag tcgccggttg
    21541 catccgcagg caggtgcagg ccgccggcaa ttttggcggt aacgcgtgcc agcgcaggct
    21601 cgaattctgc gcattcttcg ggcttcagac ggcggtacgg cacgccgtag cgttccaaaa
    21661 cggcaatgtc ttgttctgcc gcttcaactt ctttagcctg acggaaaatc tgcaacgtcc
    21721 cttttttgcg cccttcaaaa ttcatgccgg tttgcgcttc aaaacggcgg aacatttcac
    21781 ggctgtattc ggaaatcctg accatgcgct ctttattgat ttgatagcgg gatgccgtgc
    21841 agttttgcag catccgccac agccattcga tttgatacag gctgccgtcg gggcgaaaca
    21901 gcaaaggcgg atggctttta aacagccatt tcagtgcttt ggtcggaata ccgggtgcag
    21961 cccaaggcgt ggtatagccg taagaaagct ggcctgcatt ggcaaaactg gtttccatcg
    22021 ccacaccctt ggcgcggtcg atgaccgtta cttcatgtcc ggcctctgcc agataccacg
    22081 cggaagacac gccggcaaca cccgcaccta aaacaagcac tttcatgttt ctccctccga
    22141 ctttttcaaa acagacttaa tatgccgtgc cgtctgaata ttcggattca gacggcctcg
    22201 gatattaatg cggcaattcg ccgtttgtga ttttttgttt gaagtcgcgc gtttcattga
    22261 cgatgacttt cgccatcaat aaaagtgcaa tcaggttggg caatgccatc aagccgttga
    22321 atgtgtccga agccagccac accaaatcaa ggctcaacac ggtacccagc ataacggaag
    22381 aaacataacc gacgcggtac aaaccggcaa atttctcgcc gaaaacatac accgcgcatt
    22441 tttcgccgta atagcaccag cccaaaatgg ttgagtaggc aaagaaaatc aggccgatgg
    22501 taacaatcca gccgccgatg ccgggcagca ttttttggaa tgtgacggtt gtcagtgccg
    22561 cgccgctcac ttcaggtttg acaaactcgc cgcccgcgcc gagcagtccc attaccaaca
    22621 cgatgccggt aatcgagcaa acgacgatgg tatccaaaaa cgtaccggtc atagaaacca
    22681 aggcctgacg gacgggatgg tcggttttcg cggctgcggc ggcaatcggt gcagaaccca
    22741 tacctgcctc attggagaac acgccgcgcg ccacgccgta gcggatgacc gtaccgatag
    22801 caccgcccgc cactgcctgc gcgctgaacg catcggagaa aatcagcttg acggcaggca
    22861 tcagtgcatc ggaattaatc gcgataatgg aaagaccgcc caacacataa aacaccgcca
    22921 tagcaggcac gatgaaagaa gcggctttgg cgatgccttt aataccacct aaaacgacaa
    22981 cggcagtcag aacggtcaac gtaatgccgg tataggcagg ttcgataccg aagctggttt
    23041 gcaccgcctg tgcaaccgag ttggactgca ccgagctgcc gataccgaag gaagcgaatg
    23101 tgccgaacag cgcaaacgcg acggccatcc atttccagtt tttgcccaag cctttttcga
    23161 tgtaatacat cgggccgccg gacatttcgc ctttggaatt gttgacgcgg tatttcaccg
    23221 ccaacacgcc ttcgccgtat ttggtggcca tgccgaaaat ggcggtcatc cacatccaaa
    23281 ataccgcgcc cgggccgccg gttaccaccg cagtcgccac gccggcgatg ttacccgtgc
    23341 cgatggtggc ggacagcgcg gtcatcaacg ccgcaaaatg ggaaatatcg ccttcgtggc
    23401 cttcgccgct tttatgcttc tttggcggca taaacgcctg tttcagcgca taacccaaca
    23461 tcgtgaactg caaacctttt aataaaacag tcagcaaaat acccgtgccg accagcagca
    23521 tcagcatcaa aggtccccaa acccagccgc tgacggtttc aaaaaaggct ttgggattgt
    23581 ctaaaaacac ttgcatggct ttctcctttg tct
//
LOCUS       DAXHRI010000016        22465 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXHRI010000016 DAXHRI010000000
VERSION     DAXHRI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.016"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(132..311)
                     /gene="rpmF"
                     /locus_tag="ACFM75_000475"
     CDS             complement(132..311)
                     /gene="rpmF"
                     /locus_tag="ACFM75_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC6255068.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKVKGE"
     gene            complement(345..848)
                     /locus_tag="ACFM75_000476"
     CDS             complement(345..848)
                     /locus_tag="ACFM75_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC6255069.1"
                     /translation="MSDPNLIDPEVFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDMRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            996..1586
                     /locus_tag="ACFM75_000477"
     CDS             996..1586
                     /locus_tag="ACFM75_000477"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC6255070.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            1633..2358
                     /locus_tag="ACFM75_000478"
     CDS             1633..2358
                     /locus_tag="ACFM75_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC6255071.1"
                     /translation="MSEGMVSPVLYLIPTPLGTPDTPCLLPHEQRAVVGLTDFVVEAE
                     KTARAHLKHLGVTTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSIGIVSEAGCPAVA
                     DPGANLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLK
                     ALEQRSRQQNETQLFIETPYRNDALLADAVENLHPETRLCVATDLTLPTQEIISQTIA
                     QWRKRKEMPNLKKRPTIFVMYAG"
     gene            complement(2439..4076)
                     /gene="yidC"
                     /locus_tag="ACFM75_000479"
     CDS             complement(2439..4076)
                     /gene="yidC"
                     /locus_tag="ACFM75_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC6255072.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDETKPFVLFGDGKEYT
                     YVAQSELLDAQGNNVLKGIGFTAPQKQYSLNGDKVEVRLSAPETNGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSAWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(4249..4470)
                     /gene="yidD"
                     /locus_tag="ACFM75_000480"
     CDS             complement(4249..4470)
                     /gene="yidD"
                     /locus_tag="ACFM75_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC6255073.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPLGGHGHDPVP"
     gene            complement(4535..4900)
                     /gene="rnpA"
                     /locus_tag="ACFM75_000481"
     CDS             complement(4535..4900)
                     /gene="rnpA"
                     /locus_tag="ACFM75_000481"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC6255074.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRSTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            complement(4903..5037)
                     /gene="rpmH"
                     /locus_tag="ACFM75_000482"
     CDS             complement(4903..5037)
                     /gene="rpmH"
                     /locus_tag="ACFM75_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC6255075.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            5330..6886
                     /gene="dnaA"
                     /locus_tag="ACFM75_000483"
     CDS             5330..6886
                     /gene="dnaA"
                     /locus_tag="ACFM75_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC6255076.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            6966..8069
                     /gene="dnaN"
                     /locus_tag="ACFM75_000484"
     CDS             6966..8069
                     /gene="dnaN"
                     /locus_tag="ACFM75_000484"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC6255077.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPCAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(8038..8307)
                     /locus_tag="ACFM75_000485"
     CDS             complement(8038..8307)
                     /locus_tag="ACFM75_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255078.1"
                     /translation="MPSETFPFQTAWFYSNLNRADWNQKHTFAIPAQALPLYAPNPNP
                     PHSRRHCLSDSRTKPRIRLHGSGHISNTGIVLRKTVKSASASQYI"
     gene            complement(8332..10389)
                     /gene="ppk1"
                     /locus_tag="ACFM75_000486"
     CDS             complement(8332..10389)
                     /gene="ppk1"
                     /locus_tag="ACFM75_000486"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC6255079.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRKLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAREGIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIHD
                     FLLSQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNILFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALDDNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LGG"
     gene            complement(10558..11001)
                     /locus_tag="ACFM75_000487"
     CDS             complement(10558..11001)
                     /locus_tag="ACFM75_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC6255080.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(11008..11523)
                     /locus_tag="ACFM75_000488"
     CDS             complement(11008..11523)
                     /locus_tag="ACFM75_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6255081.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            11793..14423
                     /gene="leuS"
                     /locus_tag="ACFM75_000489"
     CDS             11793..14423
                     /gene="leuS"
                     /locus_tag="ACFM75_000489"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC6255082.1"
                     /translation="MQEQYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKRLNGFNVMQPMGWDAFGMPAENAAMKHNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWERETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVGEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            14658..15470
                     /locus_tag="ACFM75_000490"
     CDS             14658..15470
                     /locus_tag="ACFM75_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC6255083.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEQYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKEGFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            15463..16272
                     /locus_tag="ACFM75_000491"
     CDS             15463..16272
                     /locus_tag="ACFM75_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6255084.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFADPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            16269..16823
                     /locus_tag="ACFM75_000492"
     CDS             16269..16823
                     /locus_tag="ACFM75_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255085.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRTLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYNINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            complement(17435..17587)
                     /locus_tag="ACFM75_000493"
     CDS             complement(17435..17587)
                     /locus_tag="ACFM75_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255086.1"
                     /translation="MPDRIKPSRNGGGADGIARQQGTAQTGAKPETSPTARHGDFLRP
                     LQGGWI"
     gene            complement(17697..18008)
                     /locus_tag="ACFM75_000494"
     CDS             complement(17697..18008)
                     /locus_tag="ACFM75_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6255087.1"
                     /translation="MKYKSEALAAIHEMMEGSYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(18005..18373)
                     /locus_tag="ACFM75_000495"
     CDS             complement(18005..18373)
                     /locus_tag="ACFM75_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC6255088.1"
                     /translation="MRIFKNQWIAKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            complement(19161..19246)
                     /locus_tag="ACFM75_000496"
     tRNA            complement(19161..19246)
                     /locus_tag="ACFM75_000496"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19210..19212),aa:Leu,seq:gag)
     gene            complement(19260..19610)
                     /gene="secG"
                     /locus_tag="ACFM75_000497"
     CDS             complement(19260..19610)
                     /gene="secG"
                     /locus_tag="ACFM75_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC6255089.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(19617..20390)
                     /gene="tpiA"
                     /locus_tag="ACFM75_000498"
     CDS             complement(19617..20390)
                     /gene="tpiA"
                     /locus_tag="ACFM75_000498"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC6255090.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            20574..21077
                     /locus_tag="ACFM75_000499"
     CDS             20574..21077
                     /locus_tag="ACFM75_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC6255091.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVNA
                     YPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEYLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            21179..21835
                     /locus_tag="ACFM75_000500"
     CDS             21179..21835
                     /locus_tag="ACFM75_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC6255092.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRERFVDASLQG
                     LAYADTELPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYAAALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNIVPDVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            21915..22238
                     /locus_tag="ACFM75_000501"
     CDS             21915..22238
                     /locus_tag="ACFM75_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6255093.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(22249..>22465)
                     /locus_tag="ACFM75_000502"
     CDS             complement(22249..>22465)
                     /locus_tag="ACFM75_000502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255094.1"
                     /translation="SLHIENAAQANFQFASLVLALSHGERGLGCRLPLRFRQILTSLI
                     PRFSFAEAALSDDLLEHHRYTHLRNAV"
BASE COUNT         5075 a         5400 c         6374 g         5616 t
ORIGIN      
        1 ggtattatac ggaacgcttt gcccctgtgt gttgacgtat ggcgaaagtt tggacggatg
       61 gtgcgtccaa tgcaaaaaaa gccagtaatt gcatggcaat gttctggctt tttcagtcag
      121 tcgaataggg attattcgcc tttgactttg accactttgc ggccgcggta cataccgttg
      181 ggggagatgt ggtgcgggcg gtgtacttcg ccggttgtgc tgtcgacaga cagtgcgggc
      241 gcggtcagcg cgtcgtgcga acggtgcata ccgcgtttgg aaggggattt tttgttttgt
      301 tgaacggcca tttcaagctc ctagataaat aaactgtgtc ctaattaact gcttttcaaa
      361 cctgccaaaa cagcaaaggg gttgggtttg tcccgattga cttcttccag tgtcccattg
      421 tcgccgcagt cttcgtgtcg cggcgaaaag ggcagggaca tcaggatttg gtcttctacc
      481 aatgtgcgca tgtcgagttc tttttcaatc agtatgcctt cgagttcttc gtcggcaagc
      541 atggattcgt ccaaggactc ttcgttggaa aacaggacga tacggctgct ttcatcaagc
      601 atgaacggca tgggtttgat acatctctgg caaatcaggg gcatatcggc tttgacgttc
      661 aggtcgagga acaggcgttg cagccggtcg cgaccgccgg tcagtgtaaa cgatattttg
      721 gtctgcctgt cggcgggata atcgtgcgaa ctgacgcgtt catccaattc ttccagcaga
      781 aaactgcctt gcaggttctg cccttcggcg gcgaaaactt ctgggtcaat caaattaggg
      841 tctgacataa acgagatatg atataattta gatgttctaa cgtcaatatt tttaagaaaa
      901 atgttgcggt acgccgaagg cgggtacccg tgtttcggcg catggctgga ttttaccgcc
      961 ccttcgggcg tgttttcaac ggtaaggagg atgggatggg tttggaactg cctttgattt
     1021 tgggtacgag ttcggttttc cgccgcgaac agatggaaag gctcggcatt gcctttcagg
     1081 cggcgtcccc cgattttgac gaaacgccga tgttggggga atccgcccct cagacggcat
     1141 tgcgccttgc cgagggtaag gcgcggtcgt tggccgggcg tttcccagag gcgttgattg
     1201 tcggtgcgga ccaggtggcg tggtgcgacg gcaggcagtg gggcaagccg atgaaccttg
     1261 ctaatgcgca caagatgttg atgcatttga gcggcaggga gattgagttt tacagcgcgg
     1321 tcgttctgct aaatacggtt acgggcagga tgcagcggca tatcgataag acggtggtcg
     1381 tgatgaggaa gttggacgag ctacacatcc tccgctattt ggagcgcgag cctgatgcgg
     1441 tttattgctc gtgtgcgttg aagagcgagg ctttgggtgc attgttgatc gaaaggattg
     1501 agagtaccga tccgaatgct ttaatcggtt tgccggtttt ccgtctggtt gattttttga
     1561 aaaatgaggg cgtggaggtt ctgtaactgc ccgtttatgc cgtctgaagg gttcagacgg
     1621 catttttaac cgatgagtga gggaatggtg tctcctgttt tgtatttgat tcctacgcct
     1681 ttgggtacgc ccgatacgcc gtgcctgttg ccgcatgaac aacgggcggt tgtcgggctg
     1741 acggattttg tcgtggaagc ggaaaaaacg gcgcgcgcgc atttgaaaca tttgggcgtg
     1801 actacgccta tccgcgagct gaatctgcaa acattgaatg aacacacgga tttgaagact
     1861 ttgccggaat tgctgaaacc tttgcaagaa gggcgcagta taggcattgt cagtgaggcg
     1921 ggttgcccgg ctgtggctga tccgggtgcg aatttggtgg cattggcgca taaacacggt
     1981 tttgaagtac ggcctttggt cggtccgtcc agcttgttgc tggcgctgat ggcttcgggt
     2041 gcgaatgggc agagttttgc gtttaagggt tatttgccgt ctgaaaaaaa tgagcggatt
     2101 cagagtttga aggctttgga acaacgttcg cgccagcaga atgaaacgca gctgtttatc
     2161 gaaacgccat atcgcaacga tgcgttgttg gcggatgcgg tggaaaacct gcatcctgaa
     2221 acgcgcttat gtgtggctac ggacttgacc ttaccgactc aggaaatcat cagccagacg
     2281 attgctcagt ggcgaaaaag aaaagaaatg ccgaatttga aaaaacgccc gacgattttt
     2341 gtgatgtatg cgggttgaag atttccgtcc cgataggggg tggacaataa aaatgccgtc
     2401 tgaacaggtt tcagacggca tttttcatgc tgcggcattt aggaaacgac ttcgccttgg
     2461 gcgcgttgtt tttcgatgct gcggttgatg tgccattgct gggcgatggt caggaggttg
     2521 ttgactaccc agtacaatac cagaccggca gggaagaaga agaacatgac ggagaaaacc
     2581 aacggcatga ttttcatcat tttcgcctgc atcgggtcgg tcggcggcgg gttcagataa
     2641 gtttgggcga acatcgttgc cgccataatg atgggcagga tgtagtaggg gtcggcgcgg
     2701 ctgaggtcgg taatccaacc cagccaaggt gcctggcgca attctacgga ggcgaacaat
     2761 gcccaataca atccgatgaa gacggggatt tgcaacagca taggcaggca gccgcccagc
     2821 gggttgattt tctcgtctgt gtaaagctgc atcatcgcct gttgttgcgc catacggtcg
     2881 tcgccgtatt tctctttgat ggcttgcagt ttgggtgcgg cggcacgcat tttcgccatc
     2941 gaacggtaag aggcgttggt caatggatac agtacggctt tgacgatgat ggtcaaaacg
     3001 acgattgccc agccccagtt gccgatgatg ttgtgcagtt ggttcaggag ccagaagagt
     3061 ggggaagcga accagtgtac tttgccgtag tcttttgcca gttgcaggtt gtcggcgatg
     3121 tttgcgatga cggatgtggt ctgcgggccg gcgtagaggt tgatggaggc ttcggatttc
     3181 gcgccgtttt ggatagcggc taaaggcacg ctgacgctgg tgctgtacag cttgtcgttg
     3241 cggcgtttga tgtcaatacg gcagtcgcca gcggcgcaaa cgctttgtcc gcctttaggc
     3301 tgcaaaatcc aagcggacat aaagtggtgt tcaatcatgc cgagccagcc ggtcggggtt
     3361 ttgcggatgt attcggcctc ggatttgccg gatttggcat cgtcgtccaa gtcggagaag
     3421 ctgacttttt ggaagttgcc ttcaggggta taaacaacag ggccgacgta agagtgggta
     3481 aagtagcctt gaccctcggg ttcgctgtgg tcgcggacga tgcggtagtc cgcgctcagg
     3541 ttggcggttt gaccgctgcc gttggcgatg tcgaagcgga cgttgaccag atagctgcct
     3601 ttggtgaagg tatagacttt gtcgattttc agtccgttgg tttcgggcgc gctcaggcgg
     3661 acttcgactt tgtcgccgtt gaggctgtat tgtttttgcg gtgcggtaaa gccgatgcct
     3721 ttcagaacgt tgttgccttg cgcgtccaaa agttcggatt gggcgacgta ggtgtattct
     3781 ttgccgtcgc caaacaggac gaacggtttg gtttcgtcgc cggttgcttt gtatttgagc
     3841 agggtcagcc ggcgcaggtc gccgcttttt tcatcaatga cggcttgaac cgtgtcggtc
     3901 gttacggtaa tcggcgttgc gggcgcgagc gcggcttcgg cggaagcggt tgctgcctgt
     3961 ttttgtgccg tctgttgggg tgcggggacg ggtttcgggg tggggaacat tttttcccag
     4021 ccgatcataa tcaccagcgc gatggcgaaa aacgccgtga gtcttttaaa atccatatag
     4081 agtttcctga atggtcggaa atgccgcagg ttgcgtgttc cgatagacgg ggaataaggg
     4141 cgggcttcat acccgacggt cgtgccgcca gcccgaaggt tcagacggca ttatatagaa
     4201 accgatggga aataaaagga aagcgtgcaa tttgaatatt gcgtcgggtc agggaacggg
     4261 gtcgtgtccg tgtccgccga gggggtggca gcgtgcaatg cgcttgatgg cgagccggcc
     4321 gcctttgaat gcgccgtatt ttttgactgc ttcgacggcg tattgcgaac aggtcggcgt
     4381 atagcggcag cgcggcggaa tcagcgggct gatgcaatat tggtagaacc gtatcagtcc
     4441 cagcaggagt ttggacaata ggaagttcat cgggatgttc cttgtatggg aaacccgttg
     4501 ctgtctgaac cttgcctgca gagtaccgtt ctgatcatgc ctgtttcctg catccggttg
     4561 cggggttgcc gaacatgagt tgtgccagtt ccgcccttgc ctgttttgcg gtagacctgt
     4621 cgaatttacg gtggacgcgc acgacgaaat cctgcggcgg cagccggttt ttgttcaatc
     4681 tgaaccagtc gcggatgacg cgcttcatat aatttcgttc gttggcgcgt ttggcggttt
     4741 ttttgccgac caccagaccg atgcggggat ggtccagccc gttgccgttt gaacgcgaaa
     4801 cttgcagcag gtcgcggctg cggcggtttc tgaatgcaaa aacggatgaa aaatcatccg
     4861 ttttcaacaa gcggtactgc cttccgaagc ggtagtccaa aattataccg ccaggcgttt
     4921 gcggcctttg gcgcggcgtg cagccaatac tgcgcggccg ccgcgtgttt tggagcgcac
     4981 caggaagccg tgggtgcgtt tgcgtttggt aacggaaggt tgataagtgc gtttcatgat
     5041 gtttcctaaa aatagaatgg gtagataatt aaaccgtgaa ttacactcca atttgccgct
     5101 tttgtcaatc aatatagaaa ctttgcagga ggattgcccg attgcctgcg gatttctgcc
     5161 gcctttctgt ggataaattt tgcacgggtt gtggataaaa tatcggcgag tcggtataat
     5221 tcggttcgct gcattttgaa ccgatgtgtg ttcaacagat ttgttttctt tttgaaaata
     5281 ttatattttc tttgttttcg atttcatttt taccgattcg agcttatcca tgacattagc
     5341 agagttttgg ccgctgtgcc tccgccgtct tcacgatatg ttgcctcaag ggcagtttgc
     5401 gcaatggatt gcgcccctta ccgtcggtga ggagggtggc gtatgggtgg tgtacggcaa
     5461 gaaccagttt gcctgcaata tgcttaagag ccagtttgcc gggaaaatag aagcggtgag
     5521 ggaagagttg gctgccggcc gttccgcctt cgtattcaaa ccaggagaag gcgtgcgtta
     5581 tgagatggcg gcggttgaag gtgctgtcga acctgccgag ccgtccttgc acgcggggtc
     5641 ggaggagatg cctgtgcagg aggttctgtt ggacgagctg ccgtctgaag agcctgtcaa
     5701 acccgctgcg tcgaaaacgg cggcggatat tctggcggaa cgtatgaaaa acctcccgca
     5761 tgagccgcgc catactgccg aacctgcttc ccgtccggaa tcggaggtgc ttgcgaaagc
     5821 gcggacggat gcgcagcgcg atgcggaaga ggcacgttac gagcagacca atctgtcgcc
     5881 ggactatacg ttcgacacat tggtcgaagg caagggcaac cgccttgcgg cggctgcggc
     5941 gcaggcgatt gcggaaagcc cggggcagag ctacaacccg tttttcctgt acggcagcac
     6001 gggtttgggc aaaacccacc tggtacaggc ggtcggcaac gagcttttga aaaaccgtcc
     6061 cgatgccaaa gtgcgctata tgcattcgga cgactatatc cgcagcttta tgaaggcggt
     6121 tcgcaacaat acctatgacg tattcaagca gcaatacaag caatacgatc tgctgattat
     6181 cgacgatatt cagtttatta aaggaaaaga ccgtacgatg gaagagtttt tctatctgta
     6241 caaccatttc cacaacgaga aaaaacagct gatcctcact tgcgatgttt tacccgccaa
     6301 aatcgaaggc atggacgacc gcctcaaatc ccgtttttca tggggactga ctttggaact
     6361 cgagccgccc gaattggaaa tgcgcatcgc cattttgcag aaaaaggcgg aagcggcggg
     6421 catcagtatt gaagacgaag ccgcgctgtt cattgccaat ctgatccgtt ccaacgtgcg
     6481 cgagcttgaa ggcgcgttta accgtgtcgg agcgagcagc cgctttatga accgtcccgt
     6541 catcgacatc gatttggcgc gtaccgcttt gcaggacatt attgccgaga agcataaggt
     6601 cattaccgcc gatatcatta ttgatgccgt tgccaaatat taccgcatca aaatcagcga
     6661 cgtactcggc aaaaaacgca cgcgcaacat tgcccgtccg cgccaggtgg cgatgagcct
     6721 gaccaaagaa ctgaccactt tgagcctgcc gtctatcggc gattcgttcg gtgggcgcga
     6781 ccatacgacc gttatgcacg gcatcagggc agtggcgaaa ctgcgcgaag aagatccgga
     6841 attggctcag gattatgaaa agctgctgat tctgattcaa aactgatgga aaacgttttc
     6901 agacggcatg atattgccca tgccgtccga agggtaaaaa atccaaccga tttaaaggag
     6961 cgaacatgtt gattttacaa gccgaacgcg acagcctgct caagccgctg caagccgtta
     7021 ccggcatcgt cgaacgccgc cacaccctgc ccatcctgtc caacgtcctg attgagggaa
     7081 aaggcggtca gaccaaactc ttggcaaccg atttggaaat ccaaatcgac actgcgggtc
     7141 ccgagggcgg cgcggacgac ttccgcatca ccaccaatgc caagaaattt caggacattt
     7201 tgcgcgcgct gcctgccggt gcgctggtgt cgctggattg ggacgacagc cgtctgacgc
     7261 tgaaggcggg caaatcgcgt tttgccctgc agaccctgcc tgccgccgat tttccgatga
     7321 tgaatgtcgg cgaggacatc agcgcgactt tctcgctgga gcaggagcgt ttcaaaacca
     7381 tgctgtcgca agtgcagtac agcatggcgg tgcaggacat ccgctattat ctcaacggtc
     7441 tgctgatgca ggttgagggc agccagttgc gccttgtggc gaccgacgga caccgccttg
     7501 cctatgcagc ctgcgcgatt gatgcggatt tgccgtgcgc cgaagtgatt ttgccgcgca
     7561 aaacggtgct ggaactgttc aaactgttga acaaccccga cgatccgatt caaatcgagc
     7621 tgctggacaa gcaggtgcgt ttccaatgca acggcacgac catcgtcagc aaggttatcg
     7681 acggcaaatt ccccgatttc aaccgcgtca tccctttgga caacgacaag attttcgttt
     7741 tgtcccgtgc cgaacttttg ggcgcgctgg aacgtgtgtc cattcttgcc aacgaaaaat
     7801 tccgtggcgc gcgcctgttc ctgcaatccg gcctgttgag cgtcgtgtgc agcaacaacg
     7861 agcaggaaga agcgcgcgaa gaaatcgaga ttgcctatca gggcggcgaa ctcgaagtcg
     7921 gtttcaatat cggctatctg atggacgtgt tgcgcaacat ccattccgac gatatgcagc
     7981 ttgccttcgg cgatgccaac cgctcgacgc tgtttaccgt gccgaacaat ccgaatttca
     8041 aatatattgt gatgccgatg cggatttgac ggttttccgg agcacgatgc ccgtattgga
     8101 gatatgcccc gaaccgtgca gacggattcg gggttttgtt cggctgtcgg aaaggcaatg
     8161 ccgtctggaa tgcggcggat tggggttggg agcgtatagg ggaagtgctt gtgcgggaat
     8221 ggcgaaggtg tgtttttgat tccagtccgc cctgtttaga ttgctgtaaa accatgccgt
     8281 ctgaaacgga aaggtttcag acggcatggt ttttgattgg ggcggtgcgg gtcagccccc
     8341 gagcaaatcc cacaaatcgt tttgcagatc ggcttcgctt tcgccctcgg cgggcgcggc
     8401 gcggatatag ccgccgtcgg gctgcatcag ccatgcgtgg gtgttgtcgt ccagtgccat
     8461 ttccaaacct tcgcggatga cgcgtttttt gagttcgggc gcggtaatcg gcgcggcggt
     8521 ttcgatgcgg cggaagaagt tgcgccccat ccaatccgcg ctggagataa aggtatcgtc
     8581 cgcgccgttg ttgtggaagc aatacacgcg cgcgtgttcg agctgcctgc cgatgatgga
     8641 gcggacgcgg atgttttcgg acaagccttt tacacccggg cgcaaggtgc acataccgcg
     8701 cacaatcaaa tcgatttgta cgcctgccgc gcttgcccga tacagggctt cgatgacggt
     8761 cggttcgatg agcgaattca tcttggcggt aatccgcgcc ggtttgccgg cttttgcgtg
     8821 ttcggtttcg cgtgcgatgc ggtcgataac cattttgtgc agggtaaacg gactttggta
     8881 gagtttgttc agccgcccgg gtttgcccaa acctgtgatt tccataaaca atatgttcac
     8941 atcggcggtg atttgttcgt cggcggtaat gaggccgaag tcggtgtaga tgcgcgatgt
     9001 gccttggtgg tagttgcccg tgccgagatg ggcgtaacgc ttgagtacgc cgtcttcgcg
     9061 gcggatgacc agtgccatct tggcgtggac tttgtagccg aacacgccgt acacgacgtg
     9121 cgcgcccgcc tcttcgagct gcttcgccca gttgacgttg ttggcttcgt caaaacgcgc
     9181 catcagttcg acgacgacgg ttacttgttt gccggcgagt gccgccttca tcagggcgcg
     9241 gacgagttcg gagcgcgtgc cggtgcggta aatcgtcatt ttgacggcaa gcacgtcggg
     9301 gtcggcggcg gcttcgcgca tcatttcgac aacggggtcg aacgattggt aggggtggtg
     9361 cagcaggatg ggcgattggc gcaccaaatc gaatatggac gcggttttgc ccaaggcttt
     9421 cagtctgccc ggcgtgtgtg ggggaaattt caaatcgggg cggttgacta ggtcggggac
     9481 ggcgttgagg cgcacgaggt tgaccgggcc tttgacctga tagagttcgg cggcggtcag
     9541 tttgaattgc gacagcagga agtcgtggat gtaggcgggg caggtgtcgg cgacttcgag
     9601 ccgcacgccg tcgccgtatt cgcggtcgtg caactcgttt tgaatggcgg cgcggaggtt
     9661 ttgcaggtct tcttcgtcaa cggtcaagtc gctgtcgcgc gtcaggcgga actggtggca
     9721 gcctttgacg ttcatgcccg ggaagagttt gccgacgtgg gcgtggagga tggaggagag
     9781 gaagacgaag ccgtgtccgc cgccacacag ttcggacggc aggggaacaa cgcgcggcag
     9841 gatgcgtggt gcctgcacaa tcgccatccc cgaaggcctg ccgaacgcgt ctgtgccgtc
     9901 gagttcgacg gcgaagttga gcgatttgtt cagcgggcgc gggaaggggt gggaggggtc
     9961 aaggccgatg ggggtcagaa tcggcagcag ttcgcggtcg aaatagtctt cgatccactt
    10021 tttctgcgtg cccgtccagt tgcggcggcg gtaaaaatgg atgccttccc gcgccagctc
    10081 gggctgaagg acgttgttga acaggtcgta ctggtgccgt atcagggagc gcgccgcttc
    10141 ggtaacgtcg gcgatggttt cagacggcat tttgccgttg tccagcttgc gccgggggtg
    10201 cagcttgttt tcacgcttga gccacgccat gcggacttca aaaaactcgt caagattgga
    10261 cgacacgatg cacaggaagc gcaggcgttc caaaagggga acgtttttgt cttccgcctg
    10321 cgccaacacg cggcggttga atgccagcag gctcagttcg cggcagagga tgcggttttg
    10381 ttcgggcata aggctctccc aaagatggtt gttgttcggc cggagcgggc ggacaatgcc
    10441 gtctgaaggc ttggcggcat ctgcgccggt ttcagacggc atgatgtggc ggacggtctg
    10501 tttcaggcat cggggcaatg tggaaacgga taccggcgcg tgcggtatcc gtctgtttca
    10561 aatcacttcc aacacaaaat aatgacgcag tttttcgtac accgcatcgc tgacgttgat
    10621 gcagccgttg gtcataatcc tgtcggacac ggacggggag gcgatgcgtt cgttccgcct
    10681 ttccgacggt atctgattcc aaacgcggtg cagggcgaaa agaaaatcac cctcctgctt
    10741 gaaaccgatg acttcgccgc cgtatccggg cttgtcggta ctgttcagcg tcagcccgaa
    10801 cgtccccttg ggcgtggcaa caccgatgag gacggggtgg cactgacggt cgtcagcgaa
    10861 acagagttcc gctttggatg tgtttacgat gacttttttc ttttgaatat aagcgttgac
    10921 cgcatccggt tgcccctgtg catatacggg ggcaaccatt gacaggccgc ataatatccc
    10981 taaaaacagg cggaacggca tgtcggatta ttggcggata cgtttcggtt ttgccggcac
    11041 gatttcgcgg atgatgactt gcggctcggc tttgggtgcg ggcggcggac aggcggcatc
    11101 tttcgggaag acggggttcc agtagaagct gcgggcaaat ttgtctttat cgaaaatcac
    11161 tttgtattgg caggtagtaa catcttctac gccggaagtg ttttcagggt cgatacctac
    11221 gcccggggta tggaagtgga acaggtaatc ccattcgcgc acgccgtaca taccttcgtc
    11281 gtaatgcggg cggcccagga ttttgtagat gtcgtctttg gtcaggccgg ggcgcatctg
    11341 atccagttcg tcataagtcg ggaatgtgcc gcgcttgttg tcgagcgtta cggaataggg
    11401 tttcgggaaa accggattgt cggtcgtgcc gtcggctttg acgttgcttt tggttgcgca
    11461 ggcagacaga acgcccgccg ccaatactgc caagcccggt ttgacgattt gtttgatttt
    11521 catatgcaga atcctctttt acccgatgcc cgtctgcctt ttgttcggaa gagccgcatc
    11581 aggagagttt taaacatttg attttggttt gtaatattag cataaaaaac gtgcgtatca
    11641 ataaacgcgg tgtatttgta cggcatacgg aataatgcgc gtgcgaattt acgcatcctg
    11701 ccggcaatct gccgattcgc cgcttccggc aacctgttat aattgatgcc gactttattt
    11761 ttccaacgtt tttaagcgaa aacagaatga ctatgcaaga acaataccgg cccgccgcca
    11821 ttgagcctgc ggcgcagaaa aaatgggacg acgcccgtat tttcaacgtc tccgaagacg
    11881 cttccaaacc caaatattat tgcctctcca tgttccccta tcccagcggc aagctgcaca
    11941 tggggcatgt gcgcaactac accatcggcg acgtattgag ccgcttcaaa cgcttaaacg
    12001 gcttcaacgt catgcagcct atgggttggg acgcgttcgg catgccggcg gaaaatgcgg
    12061 cgatgaaaca caacgtcgcc cccgccgctt ggacctacga caacatcgaa tacatgaaaa
    12121 ctcagctcaa aagcctgggt tttgcgattg actgggaacg cgaaaccgca acctgcaaac
    12181 ccgaatacta ccgctgggaa caatggctgt ttaccaagct gtttgaaaaa ggcatcgtct
    12241 atcgcaaaaa cggcacggta aactgggacc cggtcgacca aaccgtcctt gccaacgagc
    12301 aagttatcga cggacgcggc tggcgttccg gcgcattgat cgaaaaacgc gaaatcccga
    12361 tgtattactt caaaatcacc gattacgccg aagagctgct caacgacttg gacaaactgg
    12421 aacactggcc ggaacaagtc aaaaccatgc agcgcaactg gatcggcaaa tctcgcggta
    12481 tgaccgtgcg ctttgctgtt tcagacgaca gcaaacaagg cttggaaggc gattacgcga
    12541 aattcctaca agtttatacc acccgccccg acacgctgat gggtgcgact tatgtcgccg
    12601 ttgccgccga gcatccgctg gcaaccgccg cagccgccga caaacccgaa ttgcaggcat
    12661 ttatcgccga atgcaaagcc ggctcggttg ccgaagccga tatggcgacg atggagaaaa
    12721 aaggcgtgcc gacaggccgc tacgtcgtca atccgcttaa cggcgacaag ctggaagtgt
    12781 ggattgccaa ctatgtattg tggggctacg gcgacggcgc agtgatggct gttccggcgc
    12841 atgacgaacg cgattttgag ttcgccacca aatacaatct gccgaaaaaa caagtcattg
    12901 ccgtcggcga caacgcattt gacgaaaacc aatggcaaga atggtacggc gacaaagaaa
    12961 acggcgtatt ggtcaacagc ggcgacttgg acggcttgga ttttcagacg gcatttgatg
    13021 ccgttgccgc caagctgcaa agccaaggcg cgggcgaacc gaaaacccaa taccgcctgc
    13081 gcgactgggg tatttcgcgc caacgctact ggggctgccc gattcccatc gtccattgcg
    13141 aacaatgcgg cgacgtaccc gtccctgccg accaattgcc ggtcgttttg cctgaaaacg
    13201 tcgtacccga cggcatgggt tcgccgctgg caaaaatgcc cgagttttac gaaactgcct
    13261 gcccatgctg cggcggcgcg gcaaaacgcg aaaccgacac catggacacc ttcatggaat
    13321 cgagctggta tttcttccgc tatatgtcgc ccaagttttc agacggcatg gtatcggcag
    13381 aagccgcgaa atactggggc gcggtcgacc aatacatcgg cggtatcgaa cacgccattt
    13441 tgcacctcct gtacgcgcgt ttcttcacca aactgatgcg cgacgaaggc ttggtcaatg
    13501 ttggcgaacc gtttgaacgc ctgctgacgc aaggcatggt cgtttgcgaa acctactacc
    13561 gcgaaaacga caaaggcggc aaagattgga tcaaccccgc cgatgtcgag ctgacgttcg
    13621 acgacaaagg ccgccccatt tccgccgtcc tcaaagccga cgggctgccc gtcgtcatca
    13681 gcggcacgga aaaaatgtcc aagtccaaaa acaacggcgt cgatccgcaa gaactgatta
    13741 acgcctacgg cgcggacacc gcccgcctgt ttatgatgtt cgccgcaccg cccgaacagt
    13801 ccctcgaatg gagcgacagc ggcgtcgaag gcgcacaccg cttcctgcgc cgcctgtggc
    13861 gtaccgttta cgaatacttg aagcaaggcg aagcggtcaa agcgttcgca ggcagccaag
    13921 acggtttgtc taaagaactc aaagacctgc gccacaaact gcacgcgacc actgccaaag
    13981 tcagcgacga ctacggccgc cgccagcagt tcaacaccgc catcgccgcc gtgatggaat
    14041 tgctcaacca atacgacaaa accgacaccg gcggcgaaca aggacgcgcc gtcgcccaag
    14101 aagtattgga aaccgccgta cgcctgttgt ggcccatcgt gccgcacatc tgcgaaaccc
    14161 tgtggagcga attgaacggc gcgaaactgt gggaagcagg ctggccgaca gtcgacgaag
    14221 ccgccttggt caaatccgaa atcgaagtga tggttcaagt caacggcaaa ctgcgcggaa
    14281 aaatcaccgt tgccgccgac gcatccaaag ccgacctcga agccgccgca ctcgccaccg
    14341 agggcgcagt gaaattcatg gaaggcaagc ccgccaagaa aatcatcgtc gtgccgggca
    14401 gactggtgaa catcgtcgtc taaccgcttt taaggtttat ccacacggat aaaggccgtc
    14461 tgaaacttgg aaacagggtt tcagacggcc tttttaaggc agatggagtt gataagtcca
    14521 ataacatatt ggaatggaaa ggttctattg gatttagact cactctacac acgcccccaa
    14581 atataaatcc attgtattcc tcacgatgtt ttggcacaat tccaagccaa tcaaccacgc
    14641 ttgtactcgg catcctaatg gcttcaaaac cttttctaaa atgggcaggc ggcaaacata
    14701 aacttgtccc ctttatcgaa cacaatctac ctactcccac acgaaaacgc ctgattgaac
    14761 ccttttgcgg ttctgccgct ttgtctcttg cattggattt cgaacaatat ctgctcaatg
    14821 atattaacgc tgacttaatc ggcttattcc gtatcctaaa agaagaaaaa tccggattca
    14881 tcgactacgc ccgctctttc tttatttccg aaaacaatag cgattcaaga ttctatgaat
    14941 tgcgggaaca attcaacttc agcaaaaatc tgcacgaacg ctcggcacta tttatttatt
    15001 tgaaccgtca tgcttttaac ggtttatgcc gttacaacag caaaggggta ttcaatgtgc
    15061 cgttcggacg ctacaaatcg ccgtactttc cccaacaaga aatggaaggt ttcattcaga
    15121 aatctgacag agttgaatta atgtgcggag attttcaaat gattctatct cagacagaca
    15181 ataccgatac ggtttattgc gatccccctt acgcgccttt aagcccaacc gcttcattta
    15241 ccacttatgc caaagaaggt ttcaatttag acaatcaaat ccgtcttgcc cagtcagccc
    15301 aacaaattgc gcccgattcc caaggcgttt tgatttcaaa tcatgatact gaattcacaa
    15361 gggatattta ttcttttgct attctgaaaa caattgaagt acaacgcaat attgccgcaa
    15421 aaggcagcag ccgtaaaaaa gtcggagaat tattggcaat ttatgattga ttttgacaaa
    15481 ccggctgaag aagacgccat ttatcaaagt cgtttgaaaa aaatcgtttc agacgacctt
    15541 gtcctctaca acgaaaattc ccttaacgtc atgcggaaga tattggaaaa atatccaaac
    15601 ggctgttttg atatgatttt cgcagacccg ccttactttc tttccaatga cggcttcagt
    15661 tgtcaaaacg gacaaatggt ttccgtcaat aagggcaact gggataaatc caaaggaatg
    15721 gcggcagatt tggaattcta cgaagaatgg ctccgactgt gttacgcctt attaaaacca
    15781 aacggcacaa tttgggtttg cggcacattc cataatattt atttaatcgg ctacctgatg
    15841 caaactatcg gataccatat tttgaacaat attacttggg aaaaacccaa tcctccccct
    15901 aatttgtcct gccgtttttt tacccattcg acagaaacaa tcttatgggc aaagaaaaat
    15961 aaaaaagcca aacacacgtt tcattatgaa atgatgaaag cacaaaatga tggcaaacag
    16021 atgaaatgtg tttggacatt tcctcctcca aacaaagccg aaaaaacatt cggcaaacat
    16081 ccgacacaaa aaccactccc cttgcttgaa cgctgcatac tctcagcttc aaacatcgga
    16141 gatttaattt ttgacccttt catgggcagc ggcacaacag gcgttgccgc cttaaaacat
    16201 ggtcggaaat tttgtggttg cgaactggaa gaagattttt ttgaattagc aaaaaaaagg
    16261 ttagaaaaat gattattggc ggaatcggtg gtgcaaggac acaaacagga ctcagattcg
    16321 aagaacgtac agacttacga acgttgtttg aagaaattcc cgggtacgac ttaagaaaaa
    16381 catatgataa tgcgggttat gaagtttggt tcaatggaga attgaaggct tattgtttta
    16441 aaaaatatga gttttaccga tttttgaaaa gaccgggata caatattaat tggaaagacc
    16501 atctgtctaa aagattacta cccgacaatg gcttatttat catcatccgt gataccttat
    16561 ttattattga gatcaaattc caacaaactc ccggttcagt agatgagaaa ttacaaactt
    16621 gcgattttaa gagaaaacaa tatacaaaat tggttcactc tttaggttgg cgggttgaat
    16681 atgtctatgt tttgaatgat tggtttacaa agccggaata taaagatgtt ttagattata
    16741 ttatcagtgt taactgccat taccaattta acacgattcc cttaaggtgg tttggactgc
    16801 ctgatggtga aacaaatgaa taaataccat atttaaaaca cagccgttca aggtcgtctg
    16861 aatatcattt cagacgacct ttcaccaatc cccgccgttt tccatctttc tgctattgtg
    16921 ataaagtagc ccgacctttt gttcaaaata cgcggattcc gcgacactga atgcagcatc
    16981 tgtcaaagat tatttgaaaa cggtcgagtt agacaagtct gccgacaatg tcgataccac
    17041 atccaaaact atccgcaggg tttaggcggt attccagagt acggtgtctt atgctgatgc
    17101 tgtaaacaat gcccgtatag aaaatctaaa acggttgttg ccgaccgtta tgccggttta
    17161 tgagcaaagt gtcagaaata aggggcgcgt taatatagtg gattaaattt aaatcaggac
    17221 aaggcgacga agccgcagac agtacagata gtacggcaag gcgaggcaac gctgtactgg
    17281 tttttgttaa tccactataa aaaacgtcgg cgttaaggga aggggatcga gaatttgagc
    17341 cgttgtttca aaatgccgtc tgaaatcgtt tgagattcag acggcattta aacagttctg
    17401 cgccccgccc gttttgcctt caagcccgcg ccgcttaaat ccaacccccc tgcaacgggc
    17461 gcaaaaaatc cccgtgcctc gcggtcgggg atgtttcggg tttcgcgccc gtctgcgccg
    17521 tgccttgctg tcgggctatg ccgtctgcac cgccgccgtt tcggcttggt ttgatgcggt
    17581 cgggcattta tgcggctacg ccctgatatt cagttcggcg gtcttgcctg ttttggcagg
    17641 catattcagc agcgcgtcta ttgtgaactt gctgattttg ccgtttgcca agtcggctac
    17701 gcaatggctt cgataccctt gtttttgacg atggtcagca gcttcaacgc cgcgccgctc
    17761 ggctttttaa cgccccgctc ccaagccgaa acgtgatttt ttcccacgtt gagatagatg
    17821 gcgaaagcgg cttgcgatag tgcctccttc tccctgattg ccttgatatc tccgccgctc
    17881 aacggtttga tttcggtcag gcaggacttg tcaaagccgc gcatagtctt tttgtcgatt
    17941 gcgccgatgt tgtaagaccc ttccatcatt tcatgaatgg cggctaatgc ctcgcttttg
    18001 tatttcatga ttctatctcc gtgataatgc cgtcttcttt caaagccgcc agctctgccc
    18061 gcgtgtattt cagataatat gcggcggctt ttcggtaaac gtccagttct ttatccgaaa
    18121 tgttttccct gtcgttcttg gcaaaggcgt aaacgaaaaa tgccctatct gcctgtttga
    18181 acaatatcag gctgcgatag ccgccgcttc tgccttgtcc ttgccttgct atgcgctgct
    18241 taatcacacc gccgcctaaa tctgcgtcta tcagcccgtt atccgcccgc tctaccgctt
    18301 ccagcagctc ggaatcgttg attttgtgct tcttggcaaa tttcgctatc cactggtttt
    18361 tgaatatccg cattgtgtag ccgttgatta gtgatacttg gaattatacc agagacagac
    18421 aacacggcaa gctgcctttt ttatgcccca caatttctcc gtttatgccg cctgaaacac
    18481 tggcggctaa cctgattatt ttttaatcaa tcgcttttaa tggctcaaat tgcgttttta
    18541 gcgcgtttcg ctgtcttcct atgcccgtat ataaccaagc ggtttaacca ccattcctgc
    18601 ctcaaaatag gcgcggttcc gtgcgttttg gcttttcatt ccgctacgct cccactgcca
    18661 agacggtgaa aaaccggaaa accccaaaag ttgttaccga ttcgttacca tttgttaccg
    18721 catttgttac caatttttta tctttttctt attttatagt gaattaaatt taaaccggta
    18781 cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc cactatagta taggtttttt
    18841 ccaaaaaccc aaaaaaaacc gctctgcatc ttggacggtt tccccctcat tccctacggt
    18901 gcgtcagtat tccccgcaag ctcttttccg cttggcattt ctgatttggc ggtcgttcag
    18961 ctttattttt gcggtatttt tcggataccg taaaaaatca ggttacttca attaggctga
    19021 attagacgga attatacaat caggaagaag acaataggat agtaatcaat cttaaagcct
    19081 tgtatttatt aggcttggtt gacggcatca gacaggatta aacgaaaaaa acgcttagaa
    19141 attctaagcg ttttgtgtgt tggtgccgac agcgagattt gaactcgcac agcctacggc
    19201 cactaccccc tcaagatagc gtgtctacca atttcaccat gtcggcattt gaaaaactgt
    19261 tatttctgct gctgaggaac aggggcagaa ggttcggtat tgcttacggg tttgggtgct
    19321 tgctgagtct gttgtacgtt gctgaagtcc aaaccgtgtt ttgtcgtgtg ggtgtgaata
    19381 tacaccatag ccatgcaggt tgcaaagaaa aatgttgctg caacggcggt cgagcggctg
    19441 aggaagttag cgttgccggc agagccgaat acgccttgcg cgctgccgct tcccgatccg
    19501 aaggtcgcgc cggcatccgc gcctttgccg tgttggagca atactaacac gatgacggcc
    19561 aaagcggaaa ttatattaac aatccaaatt agggttttga aggcttccat atttttctac
    19621 gcattttgtg cggcactgat gatggcggta aaggagtcgt acgacaatga cgcgccgccg
    19681 acgagtgcgc cgtccacata aggtactgcg aagatgtcgg ccgcgttgtc cgctttcaca
    19741 cttccgccgt aaaggacgcg gattttaaca tcgcttccgc acaaagacaa gatttctttg
    19801 tagatgaatg cgtgcatatc ggcaatctgt tcgacggtgg cgactttgcc tgtgccgatc
    19861 gcccagacgg gttcgtaggc gacggcgatg tttttggtat ccagcccttg caggatggaa
    19921 agctgatggg cgatgacttc gtgttctttg ccggcttcgc gctcttcgag gctttcgccg
    19981 acgcacaata acgggatgag tccgacgttg aggacgtttt ccattttgcg gcgttggatt
    20041 tcgttttttt cgccgaaata aaggctgcgt tcggagtgtc cgatgaggac gatgtctgtg
    20101 ccggtgtcgg cgagcatttc ggcggacact tcgccggtgt acgcgccgtt gtcggggaag
    20161 cggctcacgt cttgggcgca ggtgaggatg cggttgttta agacgatttg catggcgttg
    20221 tgcagttgca gcaggtaaac ggtcggggcg gcgagtccga tgaggacgcg ttcggcggtg
    20281 gggaggatgc ggaagcggtg catgagcgaa ttgttgttct ggagccggcc gttcattttc
    20341 cagttgccga tgacccattt ttgatcccac attccgattt ggtgatacat cttttttgct
    20401 ccgtgtcgtg ttttttctgt ctgccgcgtg tggcgcgttg caatgtgaag tttagtggat
    20461 atgcggcggg ttcgcaactt ggggcgggcg gctgcggggg cggtttggaa tgttgtttcg
    20521 ggcaggttgt tttataatgg ccgcctgata tgtatgcaaa tataggagat gtgatgcacg
    20581 cgcttcattt ttcggcttcg gacaaggccg cgctttatcg ggaggtgttg ccgcagattg
    20641 agtctgtggt ggcggacgag acggattggg tggcgaattt ggcgaacacg gcggcggttt
    20701 tgaaggaggc gttcggttgg ttttgggtgg gtttttattt ggtcgatacg cgttcggacg
    20761 aattggtttt ggcaccgttt cagggtcctt tggcgtgtac gcggattccg ttcggtcgcg
    20821 gggtgtgcgg tcaggcttgg gcgaagggcg ggacggtggt cgtcggggat gtcaacgcgt
    20881 atcccgacca tattgcctgt tcgtctttgt cgcgttcgga gattgtcgtg ccgctgtttt
    20941 cagacggccg ctgtatcggc gtgttggacg cggacagcga atatttggcg cagtttgatg
    21001 agacagatgc tttgtatttg ggcgaactgg cgaagatttt ggagaagcga tttgaggctt
    21061 cgcgtcaggc ggcttgagtg atggaaaggg gcgggcggca cgtgccgaag ttggcgcggc
    21121 gggagtgtgg ttttataatg cctgccattg ttgaaacaat tatttgacgg agcactaaat
    21181 ggattttgaa aaagcgcggt tcaatatggt cgaacagcaa atccgtcctt gggatgtatt
    21241 ggattttgat gtgttggacg ctttggcgga gattccgcgc gagcgtttcg tggatgcgtc
    21301 tttgcagggt ttggcgtatg cggatacgga gctgccgctt gccaacgggc ataagatgct
    21361 cgagccgaaa gtcgtggcgc ggttggcgca aggcttgaag ctgacgaaaa acgatacggt
    21421 tttggaaatc ggcacgggat cgggctatgc tgccgcgctg ttggcgaagc tggcgggcag
    21481 ggtggtgtcg gacgatatcg atgcggaaca gcaaaaccgc gccaaagcgg tgttggacgg
    21541 tttgggtttg gacaatatcg attatgtgca aaacaacggg ctgaccgaac tttccgcagg
    21601 tgcgccgttt gatgcggttt atgtcggcgg tgcggtcaat attgtgcctg atgtgttgaa
    21661 agaacagttg aaagatggcg gacgcatggt ggttatcgta ggccgcaagc ctgttcagcg
    21721 cgcgcttttg attacgcgca agggcgatgt gtttgaagag aaggtgctgt tcgatacctt
    21781 ggtggcgcat ttggatgata aggatgccga tccgtttggc ggtttcgatt tttaagatgt
    21841 tgacaggtca tgccgtctga aagcggattt ggggcttcag acggcatttt gcttggtttt
    21901 ttgggatttt tgcgatggat atcgtgcaac ttgggtcggc ggaattgaag gcgtggatgg
    21961 acgaagggcg gatgttttgt ttgttggacg tgcgtacgga tgaggaaacg gcggtttgtt
    22021 cgctgccaaa tgcgctgcat atcccgatga atctgattcc gctgcggcaa aacgagctgc
    22081 cggatgatgt gccgcttgtg gtgtattgcc atcacggtat ccgcagcctg catacggcga
    22141 tgtatctggc ggacgcgggg tttgaaaacc tgtacaacct tcagggcggc atcgatgcat
    22201 gggcggccga gattgacggc aacatgatgc gttattgagg caggcatttc agacggcatt
    22261 ccttaaatgc gtgtacctgt ggtgttccaa aaggtcgtct gaaagtgcag cttctgcgaa
    22321 gctaaagcgc ggtatcaacg aagttaaaat ttgcctgaac cttaaaggca gcctgcaccc
    22381 caatcccctc tccccgtggg agagggctag gacgaggctg gcaaactgaa agtttgcttg
    22441 ggcggcattt tcaatgtgca ggctg
//
LOCUS       DAXHRI010000017        22142 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXHRI010000017 DAXHRI010000000
VERSION     DAXHRI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.017"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            155..1060
                     /locus_tag="ACFM75_000503"
     CDS             155..1060
                     /locus_tag="ACFM75_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6255095.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYTLTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            1106..1255
                     /locus_tag="ACFM75_000504"
     CDS             1106..1255
                     /locus_tag="ACFM75_000504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255096.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(1474..2493)
                     /gene="lepB"
                     /locus_tag="ACFM75_000505"
     CDS             complement(1474..2493)
                     /gene="lepB"
                     /locus_tag="ACFM75_000505"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC6255097.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(2636..4429)
                     /gene="lepA"
                     /locus_tag="ACFM75_000506"
     CDS             complement(2636..4429)
                     /gene="lepA"
                     /locus_tag="ACFM75_000506"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC6255098.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(4572..5273)
                     /locus_tag="ACFM75_000507"
     CDS             complement(4572..5273)
                     /locus_tag="ACFM75_000507"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC6255099.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELVGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPARFASDGILIEAAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            5441..6571
                     /locus_tag="ACFM75_000508"
     CDS             5441..6571
                     /locus_tag="ACFM75_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6255100.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            6605..7582
                     /locus_tag="ACFM75_000509"
     CDS             6605..7582
                     /locus_tag="ACFM75_000509"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC6255101.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            7586..7936
                     /locus_tag="ACFM75_000510"
     CDS             7586..7936
                     /locus_tag="ACFM75_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC6255102.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            7941..8720
                     /locus_tag="ACFM75_000511"
     CDS             7941..8720
                     /locus_tag="ACFM75_000511"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6255103.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRAV"
     gene            8769..8975
                     /locus_tag="ACFM75_000512"
     CDS             8769..8975
                     /locus_tag="ACFM75_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC6255104.1"
                     /translation="MSALLPAACQHVSDRHEAVGMVKSASKFCIAKGGRREVKKDESG
                     GGYALCHLPDSRIVEEWEYFRSQH"
     gene            9076..9387
                     /gene="grxD"
                     /locus_tag="ACFM75_000513"
     CDS             9076..9387
                     /gene="grxD"
                     /locus_tag="ACFM75_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC6255105.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            9493..10119
                     /gene="upp"
                     /locus_tag="ACFM75_000514"
     CDS             9493..10119
                     /gene="upp"
                     /locus_tag="ACFM75_000514"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC6255106.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            10142..10462
                     /locus_tag="ACFM75_000515"
     CDS             10142..10462
                     /locus_tag="ACFM75_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC6255107.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            10713..11135
                     /locus_tag="ACFM75_000516"
     CDS             10713..11135
                     /locus_tag="ACFM75_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC6255108.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRETMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            11153..11917
                     /locus_tag="ACFM75_000517"
     CDS             11153..11917
                     /locus_tag="ACFM75_000517"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC6255109.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRALERCLVRTV
                     IAPHDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEMAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSNQ"
     gene            11989..13263
                     /locus_tag="ACFM75_000518"
     CDS             11989..13263
                     /locus_tag="ACFM75_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC6255110.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALG
                     LGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQSAIQSELDRLD
                     GNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFNQAELLPIKQAISSDLAELKNRPYVDISGTALRLDRLETAVSGLPLILDG
                     VLKPGVQVKNEAVSASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENL
                     RLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKSPATQSWLKELAELKALDVRM
                     TADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEPAAASDVKTIEAP
                     SLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            13260..14477
                     /locus_tag="ACFM75_000519"
     CDS             13260..14477
                     /locus_tag="ACFM75_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC6255111.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEALRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            14552..15616
                     /gene="hemE"
                     /locus_tag="ACFM75_000520"
     CDS             14552..15616
                     /gene="hemE"
                     /locus_tag="ACFM75_000520"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC6255112.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     MQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEAARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            15794..17173
                     /gene="radA"
                     /locus_tag="ACFM75_000521"
     CDS             15794..17173
                     /gene="radA"
                     /locus_tag="ACFM75_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC6255113.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(17449..17928)
                     /locus_tag="ACFM75_000522"
     CDS             complement(17449..17928)
                     /locus_tag="ACFM75_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC6255114.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMNIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(18053..18412)
                     /locus_tag="ACFM75_000523"
     CDS             complement(18053..18412)
                     /locus_tag="ACFM75_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6255115.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPLSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLTKGMK"
     gene            complement(18451..22065)
                     /gene="recB"
                     /locus_tag="ACFM75_000524"
     CDS             complement(18451..22065)
                     /gene="recB"
                     /locus_tag="ACFM75_000524"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC6255116.1"
                     /translation="MSAPIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYNAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQHADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGLPQ
                     DSRETVRCAYACEKDGIAMLKRNWQRLTDNAPSGTDFAFTEDVPPPAVYCGNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHNFPKGTQAGLCLHEILEDFKFGQAAARQETLIADKLKKYGFEEIWLPAVAEM
                     AEACQKTPLTGAYGLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDVRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGADASAYTQQAMDEAVTHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         5029 a         5765 c         6232 g         5116 t
ORIGIN      
        1 tcgggtttta tccggcagga cggttttttg ccccaacgga aaatagcctg cctgcccgta
       61 aaatcagccg tttgtccggg tgcagccggg gctttgggct tcagacggca tattttcgga
      121 atggcggcat tcttgccgtc ggcgcggcag ccgtatgggg aagggagggg atattgtggt
      181 cggtaacggc aaaaaatatg ccgcaccatt gctggtgctg ggttgcgtgg tgttcggtct
      241 gggcagtctg attgtcaggt ccgtccccgt cggttcgtat gcaatcgcat tttggcggtt
      301 gctgatttcg gtgttcgtat tttggttttt ggcacggttt ttcgggcaaa aattcccaaa
      361 aaacaggaaa accgtccgat ataccctgac ggcgggcgtg tttctcgctt tcgatttggc
      421 gttgtggcac gaaagcatac acgcggtcgg gccgggtatt tccaccctgc tcaacagcct
      481 gcaaatcttt ttcttgtcgg caatcggtgt tttctttttc ggcgagcgtt tgagcgggct
      541 gaaaaaggca ggcttaatat cggcagttgc cggcgtggcg atgattgccg gtgcggaatt
      601 cggctacaac ggtaatgcgg tttggggatt cgccagcggt ttggtatcgg gactgatgct
      661 cgccctgtcg atggtgttcg tccgcaaaac ccatgaaatc gagccggtgg cgcttttccc
      721 ttcaatgatg attttgagtt tgggcggcgc ggtatcgctg gttgttccgg cattgctgat
      781 ggatggcggc gcgctttatc cgacgacttg gaaagatgcg ggtttggtgc ttgtgtacgg
      841 cgtggtgatg cagtgcttcg cgtgggcgat ggttgcctat gcgattccgc tgctttcgct
      901 gtcgctgacg gggctgctgc ttttgtccga accggttgcc gccctgttca tcgattattt
      961 cgggttgggc aaaccgattg aaggcgtgca gtgggcaggg gtggcgctga cgctttcggc
     1021 aatttacctc ggttcgctga aacagcagtc cgagccttga ctccgtccgg catcagattt
     1081 tctgccgtgg caaacctgcg tacgattgaa ctggcaggca gtcaaaaccg gcaagaccaa
     1141 acacgccagc aacggcgcgg agcggaaaat gccgtctgaa gccctttcag acggcatcgg
     1201 ggcgggcaga atcaaaaaag ccgcctccgc aggaatggca atgcaactaa aataatgtcc
     1261 gtatttccat atgccgccca caccgtcagt cggacgaaat gccctttatt caatgggatg
     1321 ggtttccaca caaacggaaa tatagtggat taaatttaat ccactatatt atccccgagg
     1381 ctgccggcag ggaaacaagg cgtttgatga taaaaatgcc gtctgaaaca gggttcggat
     1441 ggcatttttg atgttccggc gtaattttcc ggcctaacgg attgccgtac cggccctgcc
     1501 gaaatcgccg aagttcatca aaatgaacat tgccttgccg acaaccagct tgtcatccac
     1561 aaatccccag tagcgagaat cggcactgtt gtcgcggttg tcgcccatag cgaaatagcg
     1621 tccttcggga actttgcaca cgaaaccgct gccgtcgtcg gcatattggc agtgttccaa
     1681 accgctttgc tctatggaat atccgttttc agacataata tcggaggtat atttgcccaa
     1741 tacgggcagg gaaacggcag gctgtccttc ttttttcaga atattgaagg atttgccgtc
     1801 tagaccgctg cggaacatat ccgtgttgtg gatttcggaa gggtcggtat cgtcgggata
     1861 acggtatgtg ccgttaggaa tatcggaagt gggtttgcca tttaccgtca aaatcttatc
     1921 ccgatattcg accacatcgc ccggaatgcc gacaatacgc ttgatgtagg tcatctccgg
     1981 ctgcagagga taattaaaaa caacgacatc gccccgttcg attttgcccg tggggatgaa
     2041 tacattgttt aaaacgggta cgcgcaagcc gtaggaaaat ttgccgacca aaatgaaatc
     2101 gcccttgatc aggcccgggc gcatggagct ggatggaatt tggaacggtt cggcaataaa
     2161 cgaccggatg agaaacaata ccaaaacggt agggaagaaa ctgccgaaat aatcgccgaa
     2221 gtggctgctt tccgagattt cgggatgagt cttcaggcgg tatttgtata ccccccaagc
     2281 cgtaccgcac aatacaacga aaatcaggaa aacggcggta aagctcataa acagggacaa
     2341 agcggcaaac acgccgaccg ctgtcaggat ataggcgtat tcgaggccgg aactccattc
     2401 accgttttcc tgccgcttct tgtcgctttt gaaataaagg atgatgccgg caagcagcgc
     2461 ggcagccgcg cccgacatta gcattgtgtt cattgttgtt ccttaatgct taaaaacccg
     2521 cctgtccgtg caaccgtttt aaggcggcaa attgcaaaat ttgtttgcgg gcgcgtgccc
     2581 ctgaaattag ggcggtttga ggggtgttcc cgacgcgccg ccctgtgtgc cagagttatt
     2641 tgtcgctcac ctgcaaaatc gccaagaacg cgctttgcgg aatttccaca ttgcccactt
     2701 gtttcatacg gcgtttacct gccttttgtt tttcaagcag ttttttctta cgcgtaatat
     2761 cgccgccgta acatttcgct aagacgtttt tacgcagtgc tttgacgttt tcgcgggcga
     2821 taatctggct gccgatggcg gcttggacgg caatgtcgaa catttggcgc ggaatcagct
     2881 cgcgcatttt cgatgccagc tcgcggcctc ggtgaaccgc gctttgacgg tgcacaatca
     2941 ggcttaaggc atcgactttt tcgccgttga ccataatatc cagcttaatc aaatcagacg
     3001 gttggaactc tttgaaatgg tagtccaacg aagcataacc gcgcgaagtg gatttgagtt
     3061 tgtcgaaaaa gtccatcacc acttcgttca tcggcaaatc ataagtcagc atcacttggc
     3121 ggcccatgta ctgcatattg acttgcacgc cgcgcttttg gttgcacaaa gtcatgacgt
     3181 tgccgacata ttcctgcggc acaaggatgg tcgcggtaat aatcggctca agaatggttt
     3241 cgatgctgcc gatgtcgggc agtttggacg ggttttcaac ttcgattttt tcgccgcttt
     3301 tcaacacgac ttcataaacc accgtcggcg cggtggtaat caaatccatg tcgaactcgc
     3361 gctccaagcg ttcctgcacg atttccaagt gcagcagacc caagaagccg caacggaagc
     3421 cgaaacccaa tgcttgggaa acttcaggct caaatttcaa cgaagcatcg ttaagctgca
     3481 atttttccag cgcatcgcgc aaggcttcgt agtcgtggct ttctaccgga taaaggcctg
     3541 cgaatacttg gctttgtact tcttggaagc cgggcagcgg ctcagaggca ggattggcga
     3601 ccaatgtaac cgtatcgccg actttcgcct gacccaattc ttttacgccg gtaatcaaaa
     3661 agcccacttc gccggctttg agttcttgtt tttgaactga tttcggcgtg aatacgccca
     3721 gctgctcaac ctgcgtttcc gccttggtgc tcataaagcg tactttgtct ttcagcttga
     3781 tggtaccgtt tttcacacgg atcagcataa ccacgccgac atagttgtca aaccacgaat
     3841 cgacaataac cgcctgcaac ggtgcatttt catcgccggt cggcgcaggg attttggcaa
     3901 cgatttcttc caaaacatct tccacaccaa tgccgctttt ggcagaacat tgaaccgcac
     3961 caacggcatc aatgccgata atgtcttcga tttcctgttc cacgcgttcg gggtcggcgg
     4021 caggcaagtc gattttgttc aaaacaggca caacttccac gcccaaatca atcgcggtat
     4081 agcagttcgc cacggtttgc gcttccacgc cttgcgacgc gtcaaccacc aaaagcgcgc
     4141 cttcgcaggc ggacaaggag cgggaaactt cataagagaa gtcgacgtgt cccggcgtgt
     4201 caatcaaatt aagctgatac acctgcccat cgcgtgcttt gtagttgagc gcggcggttt
     4261 gcgctttaat ggtaatgccg cgctcttttt cgatgtccat ggaatcgagt acctgcgtac
     4321 tcatttcgcg caaatccaaa ccgccgcaat attggatgaa gcggtcggca agcgtcgatt
     4381 tgccgtggtc gatgtgggca atgatggaga aatttcggat atttttcatt gagttgtttt
     4441 taatgctgaa cggtaggttt tagaaatgcc gtctgaacaa acagcggtac gtccaaatat
     4501 cgggcgcaac gtggaaatag cccgatattc taacggaaaa ccgctgtttt ggcataagtt
     4561 tgataaaggt cttataaaga tttgacgatt tctgccacca tttttgcaga acttgccgcc
     4621 gccgttttca agaactcgtc aaagctgatg tctgcctttt cgtctgccga atcggaaacc
     4681 gcgcggatga tgacgaaagg cgtttccaac tgatgacagg tttgggcgat tgccgccgct
     4741 tccatttcta ccgccttaac ttcggggaag tgcttgcgga tttccgccac gccttcgctg
     4801 ctgtggacga agcggtcgcc gctgacaatc aggccttgtt ctaccgccgc gccttcaaac
     4861 gtccgcgccg cccgttttgc cgcctcaatc aaaatgccgt ctgaagcaaa ccttgccggc
     4921 agttgcggca cttgtcccca aacatagccg aatgcggtta cgtcgacatc gtggtgtgcg
     4981 gtttccgtac cgatgactac gtcgccgact ttcaaaccct tgcccaaacc gcccgcgctg
     5041 ccggtgttga tgacgcagtc cggtgcgaat tgatggataa gccaagccgt tgcaaccgcc
     5101 gcgttgacct tgccgatgcc gctcaatgca agcaccatgc gttttcccac caattcgcct
     5161 tcataggcgg aaaatctgcc gaaagagacg gctttgatat tttccatcat ctcgcgcaaa
     5221 agcttgattt cttgttccat tgcgccgata acggctactg ttttcaaaga catattgctg
     5281 acctgttgtg aatttcggat agaatgccgg attatacacg ctaacacggc aggattgagt
     5341 gggggcggtt tgtccgtgcc gtctgaaacg gtttcagacg gcacggcggg tttttggtag
     5401 aatgggaagg tacagattgt ttgaagatta ggggacgagg atgtttaccg atgaaaatat
     5461 gaccgcaaag gaagaactgt tcgcatggct gcgccatatg aacaaaaaca aaggttccga
     5521 cctgtttgtg acgacccatt tcccgcccgc aatgaagctg gacggcaaaa tcacccgcat
     5581 cacggacgaa ccgctgacgg cggaaaaatg tatggaaatc gccttttcga ttatgagtgc
     5641 gaagcaggcg gaagaatttt catcgaccaa cgagtgcaac ttcgccatca gcctgccgga
     5701 caccagccgc ttccgcgtca atgcgatgat acagcgcggc gcgacggcgt tggtattccg
     5761 tgcgattacc agcaagattc ccaagtttga aagcctgaac ctgccgccgg tcttgaagga
     5821 tgtcgcgctg aaaaaacgcg ggctggttat ttttgtcggc ggcaccggct cgggcaaatc
     5881 gacttcgctt gcctcgctta tcgattaccg caatgaaaat tcgttcggac acatcatcac
     5941 catcgaagac ccgatcgagt ttgtccacga acacaaaaac tgcatcatca cccagcgcga
     6001 ggtcggcgtg gatacggaaa actggatggc ggcgttgaaa aacacgctgc gtcaggcgcc
     6061 cgatgtcatc cttatcggcg aaatccgcga ccgcgaaaca atggactacg ccatcgcctt
     6121 tgccgaaacg gggcatttgt gtatggcgac gctgcacgcc aacagcacca atcaggcact
     6181 cgaccgcatc atcaactttt tccccgagga gcggcgcgaa caattgctga cggatttgtc
     6241 gctcaacctt caggcgttta tttcgcaacg cctcgttccg cgagacggcg gcaagggcag
     6301 ggtggcggca gtcgaggtgc tgctcaattc gcccctgatt tcggagttga ttcacaacgg
     6361 caacatccat gaaatcaaag aagtgatgaa aaaatccact accctgggta tgcagacctt
     6421 cgatcaacac ctttaccaat tgtatgaaaa aggcgatatt tccctgcaag aagcattgaa
     6481 aaatgccgat tccgcacacg atttgcgttt ggcggtacag ttgcgcagcc gccgcgcgca
     6541 aagttccagc cccgatttgg aactgctctg atggcggtat ggatttccgg acggatggtt
     6601 tgaaatgatt tatccgtggc ataatgagca atggcggcag attgcggaac attgggagcg
     6661 tcgtcccaat gcatggctgt ttgccggcaa aaaagatacg gggaaaacta catttgcccg
     6721 ctttgcggcg aaggcactgt tgtgcgaaac ccctgcaccg ggctgcaaac cctgtggcga
     6781 atgtatgtcc tgccatctgt ttggacaggg aagccatccc gatttttacg aaatcacccc
     6841 cttgtcggac gaacccgaaa acggacgcaa actgttgcag atcaaaatcg atgccgtcag
     6901 ggaaatcatc gataatgtgt acctgacttc ggtacggggc ggtttgcgcg tgatcctgat
     6961 tcatcctgcg gaaagtatga atgtccaagc cgccaacagt ttgttgaaag tgttggaaga
     7021 accgccgcca caagtggtct ttttgctggt cagccacgcg gcggacaagg ttttaccgac
     7081 cattaaaagc cgctgccgga agatggtttt gcccgcgcct tcccatgaag aggcattggc
     7141 atatctgcgt gaaaggggtg tggcggaacc tgaggaacgt ctggctttcc attccggagc
     7201 gccgctgttt gatgaggcgg acggtgtccg tgcgttgcgg attaaactgt tggatatttt
     7261 ggcagaacca aggttgttga agattttgga ttacgccgcg cttttcgata aggaaaaact
     7321 tccgctcgcc gtatttgtcg ggtggatgca gaaatggctg gtcgatttgg gattgtgcct
     7381 gcaacacatg aaacccgtct attatcccgc ttatgaagac aggctgcttc agacggtatc
     7441 cggtttccgt ccgcgcaatg tatttgcggc ggaggatatg ctcaaacagc ttgcccccta
     7501 cgggtttcat actttaaatg tcaaaatgca gatcgagcat ctgctcatca actatttgga
     7561 attgaagaaa gagaacgggt gaattatgtc agacggacaa aatattccgg caaaaatgat
     7621 gtcgttgcag ctgaaagaca tgaatctgct gtacagctcc tacatgccgt ttttggaaca
     7681 cggcggtctg tttgtgcaga ccaacgacgt attttccatc ggggacgata ttctgcttgc
     7741 cgtagaaatc ctcaacttcc ccaaactgtt cctgccgacc aaagtcgcct ggatcaatcc
     7801 tgcgcgtact tcctccaaac ccaaaggggt ggggctggca ttcacaaaac acgaaaactg
     7861 cctgaaagtc aaagatcaga tcgaagtcga actgggcaac acaatcggcg gcagcagacc
     7921 tacgtttacc atgtaacgcc atgcatatca tcgattcgca ctgccacctc aattttgaag
     7981 gtttgaaaga acgcctgccc gaagttttgt ccaacatgga agcaaacggc gtggggcagg
     8041 cgctcgccat cagcgtcagc agggaaagct tctccgaagt ctttgccatc gccgaagcgc
     8101 acgaacacat ctattgcacc ataggcgtac atcccgacag caaggaagcc gaagaatttt
     8161 ctattgcgga aatggttgcc gcagccgccc atccgaaagt ggtcggcatt ggcgagacgg
     8221 gcttggatta ttactggtgc aaaggcgatt tgtcctggca acacaaacgc tttgcagacc
     8281 acatcgaagc agccaatcaa accggactgc ccgttatcgt ccatacgcgt gatgcggcgg
     8341 cggacacctt gtctatcctg aaagaatgcc gggttaattc gggcgttatc cactgttttt
     8401 ccgaagacat cggttttgcc cgtgcagcaa tggatttggg gctttatatt tctttctcgg
     8461 gaatcgttac ctttaaaaac gcacccttgg ttcaggaggc ggcgaaatac gttccggacg
     8521 accgcatttt agtggaaacc gatgcgccgt tcctcgcccc cgttcccaaa cgcggcaggc
     8581 agaacgaacc ggcttttgtg cgccataccg ccgaacatat cgccaaattg cggaaccaaa
     8641 cattggaaca ggttgcggca tatacgacgg aaaactttta ccggctgttt aaaaaagtac
     8701 ccgatatgcg ggccgtctga ccctgtaccg acgataagga aaaccatgaa ggcaattcat
     8761 ccgtatgcat gtccgcgctg ctgccggctg cctgccaaca cgtttcggac aggcatgagg
     8821 cagtcggtat ggtgaagtcc gcttccaaat tctgcattgc caaaggcggc agacgggaag
     8881 taaaaaaaga cgaaagcggc ggcggatatg ccctgtgcca tttgccggac agtaggattg
     8941 tcgaggagtg ggaatatttc cgttcacaac attgatactg cgcgatatac ggcaaatatt
     9001 gtgggaagtt tccgcttttg cgtataatgc gtcctacctg acaaattttg tcaactttat
     9061 caaaaggaat aagcgatggc ttccatccac gaccaaatta aagaagtagt aacgacacac
     9121 cgcgtcgtat tgtttatgaa aggtacgaag cagtttccgc aatgcggttt ctcttcccgc
     9181 gccgtgcaaa tcctgaacgc ggcgggctgc accgattacg ttaccgtcaa cgtattggaa
     9241 aatcccgaag tacgccaagg cattaaggaa tacagcgact ggccgaccat cccccaactt
     9301 tatgtgaacg gcgagtttgt cggcggttcg gacatcctga tggaaatgta tgaggcaggc
     9361 gagctgcaag agctgctgaa agcctgatgg attcggcaat gccgtctgaa cgtgtttcag
     9421 acggcatttt cttttccggc aaatcaaaaa aaagtataat ggcgcgtctc aaaatcacac
     9481 tggaacaccg cgatgaacgt taatgttatc aaccatccgc tcgtccgcca caaattaacc
     9541 ctgatgaggg aggcggattg cagcacctac aaattccgga cgcttgccat cgagctggcg
     9601 cgcctgatgg catacgaggc aagccgtgat tttgaaatcg aaaaatacct tatcgacgga
     9661 tggtgcggtc agattgaagg cgatcgcatc aagggcaaaa cattgaccgt cgttcccatc
     9721 ctgcgtgcag gtttgggtat gcttgacggc gtactcgacc tgattccgac tgccaaaatc
     9781 agcgtagtcg gactgcagcg cgacgaggaa acgctgaaac ccgtttccta cttcgagaaa
     9841 ttcgtggaca gcatggacga acgtcctgcc ctgattatcg acccgatgct ggcgacgggc
     9901 ggttcgatgg ttgccaccat cgaccttttg aaagccaagg gctgcaaaaa catcaaggca
     9961 ctggtgctgg ttgccgcgcc cgagggtgtg aaggcggtca acgacgcgca tcccgacgtt
    10021 acgatttaca ccgccgcgct cgacagccac ttgaacgaga acggctacat catccccggc
    10081 ttgggcgatg cgggcgacaa gattttcggc acgcgctaac tgactgattt tcggatttga
    10141 tatgaatttc caagactatc tcgccacatt tccttcaatc gaccatctgg gcggtttgga
    10201 cgttcaggat gccgacggca aaacggttca ccacattccc gccgttcagg gtaagctcgg
    10261 ttcgctcaag ctgtacaatg ctttggcaga acgttttgac ggaaaattgg acaaagaagc
    10321 ggcagaacag ggtttgatat ggtttgccga acacgttgcc gacgcgcgcg cccatcccgg
    10381 caagcatccc aatatcgacc tgcttgaaaa agttgtgcaa agcggcgaaa ccttgctgct
    10441 caagccgctt gccgcgcaat aattttcgac catgccgtct gaaatccgtt tcagacggca
    10501 ttttgtcgga aagaagaccg taaaacgggc attttctttt ctatttcagg atacgggcaa
    10561 tgatgtttca acacacagga cgacacataa agcgttgccc tatgtgttgc cctaatttgg
    10621 aaggggttac acccctctca aataacatct gatgctgctg ccacgaagga cggatgtcca
    10681 aatggcggag tttcaaccat taaggaaata cgatgaaaaa aatgttcctt tctgctgtat
    10741 tgcttctgtc ggctgccgcc caaacggtgt gggcggatac ggtgttttcc tgtaaaacgg
    10801 acaacaacaa atatatagaa gttcaaaaaa tcaaccgcaa tctttacgaa tattcgttcg
    10861 gcagtgcggc aaaaaaagaa attgccatac gcaacagcaa agctgacctg ttggggcgtt
    10921 ccgacaggtg gcaaggcatg ggtagcggac gccgggaaac gatgaaattc caaaacggcg
    10981 aatttatgta caccgtatgg acgggcttcg attccgtgac tcatacggaa agcagcggtg
    11041 ttgttgtgga gcgtaggggc aaggaagtcg cacgggttgg ctgtacgccg caaaccgcgc
    11101 aggcgaattt caacgatgct gatttttccc ggtaatcggg gcggataagg cgatggaaac
    11161 agtgaaaccc atcatgctga ttgtccgacc gtcgggcagg gcgaaggacg atgtggaaac
    11221 ctgccgtcgt gccggatggc aggcggaagt gttgagcccg attgaaatcg aagcagatgc
    11281 tgccggactg gaacttttgt ccgaacaata tgcccgtgcg gatgccgtgt tttgggtcag
    11341 tccgaccgcc gttgaaaccg ccgtcccgta ccttaacctt tcagacggca taaagatgca
    11401 catttccgta gggcagggca gccgccgcgc attggaacgc tgtttggtca gaacggtcat
    11461 tgcgcctcat gacggaaacg acagcgaggc ggttttgcgc ctgccggttt ggaacagtct
    11521 gcccgaaggt gcacgcgtat tgtttgtgcg cggacacggc gggcgggatt ttctgatgaa
    11581 tgccttgcag gagaaaggtt ttcggacgga gatggcagaa gtctatttca gacggcataa
    11641 acctttgaac tttcaaaatt tccaaaccga aaatattgcc gccgcctata ttacgtcgac
    11701 cgagctggtg cgcttgctgt tcgggcagct tccgccgcaa ttttcccgat tcttcaaatc
    11761 cttgctatac tttacccatc atccgcgcat tgcggaggca ttgaagcgcg aaggcgtgtg
    11821 ttcggtcgaa accgtcccta cgctggaagc cgcgctttcc cattcttcca tttccgtttc
    11881 agacggcatg gtctttcccg gaacctcaaa ccaataagga gcaaaacggt gggcgaacct
    11941 gaaaacaaat catccgaacc cgtacgcgag atacaggcat caaaagaaat gccgtctgaa
    12001 acctcttccc cacgcaaaga aaacgaaaca gaagtacaca ttcctgccgc tccttttatc
    12061 gtcaaacagt ccggcagcaa cgctttggcg gtctgcgccc tggtattggc ggccttgggt
    12121 ttgggcgcaa gtggtttttt gtttgtccaa ggacagaatg tcttgaaaaa ccaagagctg
    12181 gcattcaacc aaaaaatcga caaagccgcc ttgggcgagt cggaaaacgc cgccctgctc
    12241 aaagacaacc tcaaccgcca aagtgccata cagtccgaac tcgaccgttt ggacggaaac
    12301 gtcaaagcaa acggcgaaca aatcttggaa atgcaaaaat cctatcgcga actgactaaa
    12361 gggcgtgccg attggctggt ggacgaaacc gagaccatac tcaatctggc ggcgcaacag
    12421 ctggtgttga ccggcaatat ccaaacggca gttggcgtat tggagcatat cgacagccgc
    12481 ctgtcccgtt tcaatcaggc agagcttttg ccgatcaaac aggcaatcag cagcgatttg
    12541 gcagaactga aaaaccgtcc ctatgtcgat atttccggca cggcattgcg cctcgacagg
    12601 ctggaaaccg ccgtatccgg actgccgctg attttggacg gcgtattgaa gccgggcgta
    12661 caggtgaaga acgaagccgt ttccgcttca tggtggcaga acgtatggga aaaatccctc
    12721 ggcacattga aggggctggt cgaaatccgc cgtttggaaa acaacgatgc catgctgatt
    12781 tctcccgaac aggcatattt tgtgcgtgaa aacctgcgcc tccgcctttt ggatgcgcgc
    12841 actgcattaa tgcagcgcaa cagcgaagtc tatcagggcg atttgaacaa tgccgaagcc
    12901 gccgtcagac agtatttcga tgccaagtct cccgccacgc agtcgtggct gaaagaactg
    12961 gcggaattga aggcgttgga tgtgcgcatg acggcggatg acggtttgaa aaacagccta
    13021 aatgccgtcc gcgcctatcg cgacggtacg cgcatgacgg cggcggaaaa tcaagaagca
    13081 gaacaggcgg cttccgaacc ggcaaacgaa aacacagctt ccgaaccggc tgccgcatcg
    13141 gatgtgaaga ccatagaagc accgtccctg ccttcggaac gcaaaccgga acagcctgca
    13201 aaaaaacagc ccgtaccgga aaaggcaggg cgttcgccgt ccgctaaagg agaacgcgca
    13261 tgaaaacggt agtctggatt gtcgtcctgt ttgccgccgc cgtcgggctg gcattggctt
    13321 cgggcattta caccggcgac gtgtatatcg tactcggaca gaccatgctc agaatcaacc
    13381 tgcacgcctt tgtgttaggt tcgctgattg ccgtcgtggt gtggtatttc ctgtttaaat
    13441 tcattatcgg cgtattcaat atccccgaaa agatgcagcg tttcggttcg gcgcgtaaag
    13501 gccgcaaggc cgcgcttgcc ttgaacaagg cgggtttggc gtattttgaa gggcgttttg
    13561 aaaaggcgga actagaagcc ttgcgcgtgt tggtcaacaa agaggcgggc gacaaccgga
    13621 ctttggcatt gatgctgggt gcgcacgccg ccggacagat ggaaaacatc gagctgcgcg
    13681 accgttatct tgcggaaatc gccaaactgc cggaaaaaca gcagctttcc cgttatcttt
    13741 tgttggcgga atcggcgttg aaccggcgcg attacgaagc ggcggaagcc aatcttcatg
    13801 cggcggcgaa gatgaatgcc aaccttacgc gcctcgtgcg tctgcaactt cgttacgctt
    13861 tcgacagggg cgacgcgttg caggttctgg caaaaaccga aaaactttcc aaggcgggcg
    13921 cgttgggcaa atcggaaatg gaacggtatc aaaattgggc ataccgccgc cagctggcgg
    13981 atgctgccga tgccgccgct ttgaaaacct gcctgaagcg gattcccgac agcctcaaaa
    14041 acggggaatt gagcgtatcg gttgcggaaa agtacgaacg tttgggactg tatgccgatg
    14101 cggtcaaatg ggtcaaacag cattatccgc acaaccgccg acccgaactt ttggaagcct
    14161 ttgtcgaaag cgtgcgcttt ttgggcgaac gcgatcagca gaaagccatc gattttgccg
    14221 atgcttggct gaaagaacag cccgataacg cgcttctgct gatgtatctc ggtcggctcg
    14281 cctacggccg caaactttgg ggcaaggcaa aaggctacct tgaagcgagc attgcattaa
    14341 agccgagtat ttccgcgcgt ttggttctgg caaaggtttt tgacgaaacc ggagaaccgc
    14401 agaaggcgga ggcgcagcgc aacttggttt tggcaagcgt tgccgaggaa aaccgacctt
    14461 ccgccgaaac ccattgaaac cttcagacgg catcgttgaa atgccgtctg aaaaccattt
    14521 cacttgacaa acagactgac agaaaacccg tatgactctt ttaaaaaacg ataccttcct
    14581 ccgcgccctg cttaaacagc ccgtcgaata cacgccgatt tggatgatgc gtcaggcggg
    14641 gcgttatctg cccgaataca aagccacacg cgcgaaagcg ggcagcttcc tcgatttgtg
    14701 taaaaacacc gaattggcga ccgaagttac catccagccc ttagaacgct tcgatttgga
    14761 cgcggcgatt ctgttttccg acatcctgac cgtccccgac gcaatgggct tgggactgta
    14821 ttttgccgaa ggcgaaggcc cgaaattcaa acgcgccatg caacacgagg ccgacatcgc
    14881 caagctgcac gttcccgata tggaaaaact gcaatacgtt ttcgacgcgg taacttccat
    14941 ccgtaaagca ttggacggcc gcgtaccgct catcggcttc tccggcagtc cgttcacgct
    15001 cgcctgttat atggtcgaag gcggcggcag caaagaattc cgcaccatca aaaccatgat
    15061 gtactcgcgc cccgatttgc tgcacaaaat cctcgatacc aacgcccaag ccgttaccgc
    15121 ctacctcaac gcccaaatcg acgcgggcgc gcaggcggtg cagattttcg acacttgggg
    15181 cggcgtgttg agcgacgcgg cgtttaaaga attcagcctc aaatacatcc gccaaattgt
    15241 tgccggactc aaacgcgaaa gcgaaggcag gcgcgtgcct gttatcgtgt ttgccaaagg
    15301 cggcgggctg tggctggaaa gcatggcaga aatcggcgca gacgcattgg gcttggactg
    15361 gacgtgcaac atcggcgaag cacgccgccg cgtcggcaag caagtcgccc tgcaaggtaa
    15421 cttcgacccg ttcgccctct tcggtacgcc ggaatccatc cgcaccgaag ccgcacgtat
    15481 cctagccgac tacggacacg gcagcggcca tgtcttcaac ctcggacacg gcatcaacca
    15541 acacgccgac cccgaacacg ccaaaatctt agtcgatacc gtacacgagc tgtcgcggca
    15601 gtatcacggc gggtaagccg gcaggaaacc gcccgatatg ccgtctgaaa gccgagggat
    15661 ggaaaattag gtaatacggc aatatccgcc gtgtacggat agtacatgac ggcggcggtg
    15721 ttgccgtatt ggcgtaatcc caaccgtccc tattttcaga cggcattttt gttttcagat
    15781 gcagggaaaa ccgatggcaa aaacgcttaa aaccctttac caatgcaccg aatgcggcgg
    15841 cacttccccg aaatggcagg gcaaatgccc gcattgcggc gagtggaaca cacttcagga
    15901 aagcttggcc gcgcccgagc cgaaaaacgc ccgcttccaa tcttgggcgg cggatacctc
    15961 gaccgtccaa tccctctcct ctgttaccgc gaccgaagtg ccgcgcaatc cgaccggcat
    16021 gggcgaactc gaccgcgtat tgggcggcgg tttggtcgat ggtgcggtca tcctgctcgg
    16081 cggcgatccc ggcatcggca aatccacgct gctgttgcaa accatcgcca aaatggcgca
    16141 aagccgcaaa gtgctgtacg tttccggcga agaatccgcc caacaagtcg ccctgcgcgc
    16201 gcagcgtttg gaactgccca ccgaaggcgt gaacctgctt gccgaaatcc gcatggaagc
    16261 gattcaagca gcgttgaaac agcatcagcc cgaagtcgtg gttatcgatt ccatccaaac
    16321 catgtattcc gaccaaatca cttccgcccc cggctccgtg tcgcaggtgc gcgaatgtgc
    16381 cgcccaactg acgcgtatgg cgaaacaaat gggcatcgcc atgattctgg tcggacacgt
    16441 gaccaaagac ggcgcgattg ccggcccgcg cgtgctggaa cacatggttg ataccgtgct
    16501 gtatttcgag ggcgaccaac attccaacta ccgcatgata cgcgccatca aaaaccgttt
    16561 cggcgcggca aacgaattgg gcgtattcgc catgaccgaa aacggtttga aaggcgtgtc
    16621 caacccgtcc gccatcttcc tcgccagcta ccgcgacgac acgcccggtt cgtgcgtttt
    16681 ggttacacag gaaggcagcc gcccgctttt ggtcgaaatt caggcattgg tcgatgacgc
    16741 gcacggcttc acccccaaac gcctcaccgt cggactggaa caaaaccgcc ttgcgatgct
    16801 gcttgccgta ttaaaccgcc acggcggcat tgcctgtttc gaccaagacg tgtttctcaa
    16861 tgccgtcggc ggcgtcaaaa ttggcgaacc cgccgccgac cttgccgtca tccttgccat
    16921 gctctccagc ttccgcaacc gccccatgcc cgaaaaaacc gtggttttcg gcgaaatcgg
    16981 cttaagcggc gaagtccgcc ccgtcgcacg cggacaagag cggctcaaag aggcggaaaa
    17041 gctcggcttt aaacgcgcca tcgtcccaaa agccaatatg ccgcgcaacg ccaaagagtt
    17101 tccgaacctg aaaatctacg gcgtttcgag tttgcaggaa gccatcgata tttgccgtga
    17161 cagcagggaa taaacggaaa tgccgtctga aatcaggttt cagacggcat ttggtttgtg
    17221 gcggattgaa acaagaaggc ataccggcga cagataagat ttgtggcaaa gttgcctgtg
    17281 atgtggcaaa aacacacacg cccgtcatcc ccgcaagggt gggaatctgg agtcgtccgt
    17341 ttctgcaatg attgaaaatc acggtaaccc aaccgattgg attcccgact tcgtgggaat
    17401 gaggggcgtg tgcatttgat ttccatccgc catatgtcgg cgacgggctt attcgcctac
    17461 ggttttttgt atcagttttt cggcgtttgc caaagtgttt gccacttcgt caaaaccgat
    17521 gcggctgccg gcgatgaggg cgcgcgtatc ggtataggcg gcgcgtactt tgccgtctgt
    17581 ttcggtaacg aggacgcgca ggggcagttg cagggcgaag gcggggtctt tgaccatcag
    17641 cggcgtgccg gctttgggcg tgccgaagac gatgactttt gccggctgca tcgttaagcc
    17701 gtttcggcgg gcggcttcct gatggtcgat gacggcaaaa atgttcatcc ctttgctttt
    17761 tatggcggtt tcaaggcggc tgacggtttc gtcaaagctg tattttgagg tgagggtatg
    17821 cgtggtcata gcggtttcgt tttgggtttg cggttcgctg gcaggatgtg ccgaagcggt
    17881 tgaaatgcag agtgcggatg cggcaatcag ggggagtatg tgtttcatcg tatttccttt
    17941 tcggtttgtt tatcaggctt tggacggggc ggcacgcgcc gcgcccgatg ttgcgccgcg
    18001 tgccggaacg ccgtatgccg tctgaaagcc tgtcctttca gacggcattg cgtcatttca
    18061 tcccttttgt gagcaggtct tcataaccgc cgtgattggc aacatttgta taacctgctt
    18121 ttttcagttc ttgaagggcg gcttcggcac gccgtccgct gcggcagtag aggttgaccg
    18181 gcgtgtcttt gtcgggcgcg gcttcgtgta tgcggcggac gatttggtcg acggggatgt
    18241 tgaccgcgtt gtgcaaatgc ccttcgctaa attcctgttc ggaacggaca tcgatccaaa
    18301 cggccggatg ttgcgcggtt tgggcggcgg ataggggttt ttgcggggct gcctgcgcgg
    18361 caaaggcggc tgaggcaatg agtgcggcgg taatcaggtg tttgatattc atagggttct
    18421 cctgcggttg tcgttttaag ggacgggaag ttattttatc tgttccaaag cggcggcatc
    18481 tatgtcccaa cgccaaacgc cgccgccttt gccatccaat ccgcgcaaaa acaggtagcg
    18541 gacggaaacg gcggcgggcg gttgtccgcg cagtttgaag tagcgtgcgg cggcaacggc
    18601 gtaaatcagc gcctgaaggt aatagtggtg gtgcgtgacg gcttcgtcca tcgcctgttg
    18661 cgtgtaggcg gatgcgtccg cgccgaggtg gttcgacttg tagtcgatga cgcagatatt
    18721 gccgtcgggg tcttggcaga ccatatcgat aaagccgttt aaaaagccgt tgacggtgtg
    18781 gaagtctagc gtttcggcgg cggcacggca gacttcgggc agccggacat cgtcgcgggc
    18841 aaaccagtcg cgcagccgtt tgaggccgaa gtcttcggtg tggagggtaa agcccatttc
    18901 gggacagcgg cactcgggcg ggatgtcgga cagaccgtat gcccccgtca gcggcgtttt
    18961 ctggcaggct tccgccattt cggcaacggc gggcagccat atttcttcaa atccgtattt
    19021 tttcagcttg tcggcaatga gggtttcctg tctggcggct gcttgtccga atttgaaatc
    19081 ttcaagaatt tcgtgcaggc acagccccgc ctgcgtgcct ttcgggaagt tgtgtatcga
    19141 tatgccgtct gaagccgtcg gcgtttcaga cggcatcgcc ggcaccgagg tttcggcggc
    19201 atccaaggac gggcaggcat cttcttcccc gccgtcgggc gtttgggtat ggcggcttaa
    19261 ggcggtaaag ctggtgtggc ggacaaatcg gaatccgcgt tcgggaatgc tgtttgcggc
    19321 aaattcggcg gtttgaccgg cattgccgca atatacggca gggggcggca catcttctgt
    19381 gaaggcgaaa tccgtgccgg aaggggcgtt gtccgtcaaa cgctgccagt tgcgtttgag
    19441 catcgcgatg ccgtcttttt cacacgcata ggcacagcgg acggtttcgc ggctgtcttg
    19501 cggcaagcct tcaattaggt aggcgagggg gttgtcggcg gtattggtgg agtatgcggc
    19561 gtagatgttg agctgttcct cggcacgcgt cagtgcgaca taaagcaggc gcaggcgttc
    19621 cgccatttct tcgtcggcgt gttgcttctg ttcgtcttcc gacagttgcg cctttgccaa
    19681 cagttcggtt cggtttgcgc tttggtggag gatttgccag tcggacggcc cggtatcttg
    19741 cgcgtcccac gcaaacgggc agtacaccag cggatactgc aaacctttcg aggcgtgcat
    19801 ggtaacgatt ttgaccaaat cttcgtcgct ttccagacgg atggcgcggt tgtcgccgcc
    19861 gttgttgccg gccaggctga tttggtcgcg cagccatttg tgcagcgcgg cggggttgcg
    19921 gttttgcgcg tcttcggcgg caagcagttc gagcagttgg aaatagttgg tcaggctgcg
    19981 cccgttgttc cggcttaaga ggcgtgtttc gatgccgtgt gtttgggaaa attgctgcat
    20041 agcggcgaaa atgccgtatt gattccagtt gtcgagcgcg gttcgggcag attccgccca
    20101 atgcaaaatc tcgctttcgt tttggttgaa gtcgtgcaat tgctgcgcgt tatagccgaa
    20161 tatgctgctt gtcaggacaa agcgcagcgt tccggcgcgg cgcggttcga gccagaagcc
    20221 gatgagtgcg gacagggcgg cggcttcggg cgaggcgaac acggattcgc gcgaaagcag
    20281 gacgctttgc acctgccgtt ttttcagggc ggcggaaacc atcaccgcct cgttgtgcgt
    20341 gcgtaccagc acggcaatat cgcccgactg caacgggcag cctttgaaat tcagacggcc
    20401 tccggcggct tcgttgagcg cgtgggcgat ttcgtcggcg caatagtcgg cggcacgtcg
    20461 gcgcaaaacg tctttgttgg ctttttcatt gtcgttttcg tgcagccaac gaacctgtac
    20521 ggcaggacgt ttgggcgaca atctgctttc ggcacgcgcc gcaccgactt ccgaatagcc
    20581 gatgttttcc aaaacgaacg ggcgttcttt gaggcggaac agtgcgccta tgctgccgat
    20641 aagcgcggcg tggctgcggt agttggtggc gagcgtgtag cggtgccgcg cgtcttccgc
    20701 tgcctgaagg taggcgtaaa tgtccgctcc gcgaaagctg taaatcgcct gtttgggatc
    20761 gccgaccaga aacagcgggc ggttttgggc gatgaagatt ttttggaaga tttcgtattg
    20821 cagcgggtcg gtgtcttgga actcgtcgat cagcgcggtt tcccagtttt cggcaacggc
    20881 gcgggcgagg gtttcggcgt gcggattgtc ggtcagcgcg gtgtggacat cgagcagcag
    20941 gtcgtcgaaa ccgcgttcgc ggcgcgattt tttcatctcg gcaaggctgc ggttgaggta
    21001 ttcgattaaa tccagttgca gccggatcat tgttgcttct tccgcttctt cgagtgcgtt
    21061 caaatcgcgc ccgaagtctg ccagtttctg caattcggca aatactgccg catcgggcgt
    21121 tttgcctttt ttcagtccgg cttcgagttt gtcggacgca agtttcaaga gtctgtcgtg
    21181 tgtgtctttg tccagaaagg gcagttgtcc ggcggcggat ttttgtgcca gttctttaaa
    21241 aaggttgccg aagctgtttt tgcggtaact gttgccgttg aggtcgggat gaatgcgcca
    21301 aaagccggct tccagttctg gcagcaggcg gcagatggtt tgccatgagg tttcggcgtt
    21361 gcgctgcgcc tgtttcaaat ccgcctgcgg acggcggaaa ttcaggtacg ggcgggaaag
    21421 atagcgcgaa atttgggcaa ggacggtttg cggcacagct ttgcgtttaa acgccaatgc
    21481 ggcaagcacc ggatcattgc tgacgcgttc ccgccaaaaa tcttgcgccg ggataagcag
    21541 gcggtcgccg tcttcttcgg tcagttcgac atcgaacggt gcttggcaca ggaaggcgta
    21601 gtcgcgcagg atgcgctggc agaagccgtg gatggtatag atggcggcgt tgtcgaattg
    21661 cccgatggcg gccttgaggc ggacaatcag acgcgtccgg ccctcttttt gcaaagcctg
    21721 ttttaagagt tcgggcagga aggtgtcgcc ttcgtggtgt tcggcgcagt aggcggcgat
    21781 gccgtctgaa agcgtgtcgt ctccaagttt ggcaattcct ttgctttcta aaacttgtaa
    21841 cacatcgtcc aaacgtgcgc gcaggcgtgt tttcagctcg gcggtggcgg ctttggtaaa
    21901 ggttacgacc aatacgcgct cgacgctttt ttgttccaat acgattaggc gcgtaaacag
    21961 ggcggcaatg ccgtaggttt tgccggtgcc ggcagaggcc tcaatcaggt tggtgccgga
    22021 aatggggacg gtcagcgggt cgaatgcttg gatgggtgca gacatagtgc gcgctcggaa
    22081 aacggttgga cggtatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
    22141 gt
//
LOCUS       DAXHRI010000018        20905 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXHRI010000018 DAXHRI010000000
VERSION     DAXHRI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.018"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(61..1947)
                     /gene="uvrC"
                     /locus_tag="ACFM75_000525"
     CDS             complement(61..1947)
                     /gene="uvrC"
                     /locus_tag="ACFM75_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC6255117.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     ILQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDVVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSEIPGIGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(2002..4164)
                     /locus_tag="ACFM75_000526"
     CDS             complement(2002..4164)
                     /locus_tag="ACFM75_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6255118.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEF
                     AELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAQQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4303..5253
                     /gene="trxB"
                     /locus_tag="ACFM75_000527"
     CDS             4303..5253
                     /gene="trxB"
                     /locus_tag="ACFM75_000527"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC6255119.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLEINLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLETDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5409..5777
                     /gene="rpsF"
                     /locus_tag="ACFM75_000528"
     CDS             5409..5777
                     /gene="rpsF"
                     /locus_tag="ACFM75_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC6255120.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAAAQ"
     gene            5778..6080
                     /gene="priB"
                     /locus_tag="ACFM75_000529"
     CDS             5778..6080
                     /gene="priB"
                     /locus_tag="ACFM75_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC6255121.1"
                     /translation="MGFNNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQKENGQ
                     QCFVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            6087..6317
                     /gene="rpsR"
                     /locus_tag="ACFM75_000530"
     CDS             6087..6317
                     /gene="rpsR"
                     /locus_tag="ACFM75_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC6255122.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6334..6786
                     /gene="rplI"
                     /locus_tag="ACFM75_000531"
     CDS             6334..6786
                     /gene="rplI"
                     /locus_tag="ACFM75_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC6255123.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(6980..7786)
                     /locus_tag="ACFM75_000532"
     CDS             complement(6980..7786)
                     /locus_tag="ACFM75_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6255124.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKRAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLINGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(7787..8533)
                     /gene="pssA"
                     /locus_tag="ACFM75_000533"
     CDS             complement(7787..8533)
                     /gene="pssA"
                     /locus_tag="ACFM75_000533"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC6255125.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            9116..9598
                     /locus_tag="ACFM75_000534"
     CDS             9116..9598
                     /locus_tag="ACFM75_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC6255126.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(9650..10861)
                     /locus_tag="ACFM75_000535"
     CDS             complement(9650..10861)
                     /locus_tag="ACFM75_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC6255127.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11084..13522
                     /locus_tag="ACFM75_000536"
     CDS             11084..13522
                     /locus_tag="ACFM75_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6255128.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNVDSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            13733..15043
                     /gene="tig"
                     /locus_tag="ACFM75_000537"
     CDS             13733..15043
                     /gene="tig"
                     /locus_tag="ACFM75_000537"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC6255129.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINEMVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SLKIAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRAIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQV"
     gene            15139..15753
                     /gene="clpP"
                     /locus_tag="ACFM75_000538"
     CDS             15139..15753
                     /gene="clpP"
                     /locus_tag="ACFM75_000538"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC6255130.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(15810..17075)
                     /gene="ispG"
                     /locus_tag="ACFM75_000539"
                     /gene_synonym="gcpE"
     CDS             complement(15810..17075)
                     /gene="ispG"
                     /locus_tag="ACFM75_000539"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC6255131.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(17091..17852)
                     /gene="pilW"
                     /locus_tag="ACFM75_000540"
     CDS             complement(17091..17852)
                     /gene="pilW"
                     /locus_tag="ACFM75_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC6255132.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQEYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(17855..18949)
                     /gene="rlmN"
                     /locus_tag="ACFM75_000541"
     CDS             complement(17855..18949)
                     /gene="rlmN"
                     /locus_tag="ACFM75_000541"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC6255133.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTSAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19092..19517)
                     /gene="ndk"
                     /locus_tag="ACFM75_000542"
     CDS             complement(19092..19517)
                     /gene="ndk"
                     /locus_tag="ACFM75_000542"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC6255134.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(19576..20727)
                     /gene="zapE"
                     /locus_tag="ACFM75_000543"
     CDS             complement(19576..20727)
                     /gene="zapE"
                     /locus_tag="ACFM75_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC6255135.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         4991 a         5190 c         5721 g         5003 t
ORIGIN      
        1 atcggtatac cgacctgctg tatcatattt tttacagacg gtttttgctt gggcagcata
       61 ctaatgcaga tgctcgtaaa tggtttccgc caatgccttg ctgatgcctt ccactttttc
      121 caagtcctcg cggctggcgg caatcacgcc acgcaggccg ccgaagcggg tgagtagggc
      181 ttggcggcgt ttgctgccga taccggggat ttcgctgagt gaggaagtaa cgcgtgcttt
      241 gtcgcgtttt ttgcgatggc ctgtaatcgc aaagcggtgc gattcatcgc gtacggtttg
      301 caataaatgc aaggcagggc tgttgggtgg caggcggaag acttcgccgg taaaaggcag
      361 tataagttct tccataccgg ctttgcgctc agggcctttg gcgataccga ccaaaggaat
      421 gtgcagcccg agttcttccc atacggacac ggcgatgccg atttgccctt tgccgccgtc
      481 aatcaatacg acatcgggcc atttgacggt ctcgccgttg gcttcggctt cctgcatttt
      541 gccgtaacgg cgcgtcaaca cttcgcgcat ggcggcgtag tcgtcgccgg gtttggcggt
      601 cgtgatgttg tagcggcggt attgcgaagg ctggatgttt tgctcatcgt acacaacgca
      661 ggacgcaata gtggcttcgc cttgggtgtg gctgatgtcg aaacattcga ggcggttgag
      721 gccgtctgaa tccatgccga ggattttggc cagttcatca atacggtgtt gttggctgct
      781 ttgttgcagg cggcgttggg caatcgccat ttgtgcgttt tgctccgcca ttttcaacca
      841 gactttgcgt tcgcctatgg ttttggtaac gaactgcatt tgtttgccgt gttcgctctc
      901 tagggcttct ttcaatgcgt cgggcatggg gaagttgctg atgatgatgt cgggtttgct
      961 tttgcccaga tagtgttggg cgacgaaggc ttcggcatag tcttgcccgt ttggctcggg
     1021 atcgtttttg gtgtcgggga agaaactttt gtctccgacg tgccgcccac cgcgtatgct
     1081 gacccagtgt acgcagacga tgccgtctga aaccgccagc gcgagcaaat cgatatcgtt
     1141 ggggttgttc gggtttttgc tgtcgatgaa ctggttgctc tgcataatgc cgagtgcttg
     1201 gatttggtcg cggtagcggg cggcttcttc aaattgcaga ttcgcggcgg cagtttgcat
     1261 tttgtgttgc agggtgcggg tcagttcgtc ggttttgccg ttgagaaaag tggcggcttg
     1321 acgcacgctg tcgcggtagt cttcttcgct gatgtgtccg acgcagggcg cggtacagcg
     1381 tttgatttga tacagcaggc aagggcggtc acgatgctcg aatacgctgt cttcgcaggt
     1441 acgcagcatg aagacttttt gcaggatttg gatgctgtcg cgcacagcgt tgctgttggg
     1501 atacgggccg aagtattggt tgggcttttt cagcgtgccg cggtaatacg ccatttgcgg
     1561 atattgatgg ccgctgagca tcagataagg atagcttttg tcatcgcgga aaaggatatt
     1621 gtatttcggc gacagggctt tgatgaagtt gttttcgaga atcagggctt cggcttcgga
     1681 acgcgtgatg gtggtttcga tatgatgaac ctgttttacc atcaatgcga tgcgcggcga
     1741 atggtcgttt ttttggaaat agccggacac gcgccgcttg aggttgaccg ctttgccgac
     1801 gtataagact ttgccgcctt cgtcaaaaaa acggtacacg cccggcagct tgggcaggtt
     1861 tttgaggaag agcgggatgt cgaattgttc cggagaacgg ttttctgtat tcatatcaat
     1921 catttgaaaa acggcttcag acggcatatc ggcagcggca gccttatccg ttcggaaaag
     1981 ctgtcgggtg gttatatgcg gtcaatcgat ttttacccgt ttcaggcgca aggcattgct
     2041 caacacggaa accgagcttg ccgccatggc cgcgcctgcg atgacgggat ttaaaaagcc
     2101 aagcgcggcg agcggaatgc ccaagatatt gtagaagaag gcgaaaaaca ggttttgctt
     2161 gatgtttttc aaagtggctt gcgacaccag cagggcatcg gcgagctgat taaccgaatg
     2221 ctgcatcagc gtggcggacg cggtgtgttc ggcaacgtcc gcaccgcctt tcatggcgaa
     2281 gctgacgttt gcggcggcga gcgcgggcgc gtcgttaatg ccatcgccga ccatcgctac
     2341 ggttttgccg gcggctttga gtttctgcac ttcggcggct ttgtcgcgcg gactcatatt
     2401 gccgaaggcg tgagcgatac ccagttgttg ggcgacgtat tcgaccgtgc cttggttgtc
     2461 gccgctcatg atgtaaacat cgatattgtg ttttttcaga cggcctatgg cttcggcggt
     2521 atcggctttc aacgcgtcgg caagtgcgaa tgcgccgatg ggtttgttat cgactgagac
     2581 cgcaacaatg cttgcaatat cccaaacgcc gtctaaaaac ttcggcaagg ccagttcggc
     2641 aaattcggct ttgcctgctt tcaccaaacc cacgccttcc acttcggcgg taatgcctgc
     2701 gccgacaacg gtttgtgcgt tttgtgcggc gggaatgtcc aaaccgcgcg cttgggcggc
     2761 ggagacgatg gcacgggcga gcggatgggc ggcgttttgt tcgacggcgg cggcgatgcg
     2821 gtacaaagcg tcttcgtcaa agccgctgtc gggaacgcaa taaacggcgg caacctgcgg
     2881 gctgccttcg gtcagcgtac cggttttgtc caacacgacg gcatcgacgt gggcggcttc
     2941 ctccattgct gccgcgtctt taaaccaaat accgtgttta accgctttgc ccataccgac
     3001 cataatcgcg gcaggggttg ccagacccag tgcgcacggg caggcaatca ccaaaacggc
     3061 gacggcgtgc atcagcgcaa ccgtccaatc gcccttaatc agccaagtaa caataaaagt
     3121 caacaacgca atgcccacga cggcaggcac gaataccgca gccgctttat cggctacgcg
     3181 cgcaatcggt gctttactgc cttgtgcttc agagagcgcg ttcatcatgt cgccgagctg
     3241 ggtttggctg ccgagctgcg tggcgcggta caccacactg ccttcggtca ttaacgcgcc
     3301 cgccaacact ttgccgcccg cctttttttc ttcaggattg gattcgccgg taagatggct
     3361 ctcgtccgcc caaccgctgc cgctttcaat gatgccgtct gcggcaatgc gttcgccgtg
     3421 gttggcgcgg ataaggtcgc cgatttgcac ttggtcgatg ggaagctgtt tccattcgcc
     3481 gttgcgttgc acgttgactt gggttggtgt aagtttgagc agcaagccca agctgttgag
     3541 gctggatttt ttggtacggt gttccaaaaa tttacccagt gacacaaaac cgatcaccat
     3601 cacgcccact tcaaaataca catgcgccat accgtacgcc gcgtgcgggc tgaaaaacag
     3661 catatagacg gaatacaggt aaatcgagac cgtgccgatg gtaaccagca cgtccatatt
     3721 cgccagtccg cccttaatgc tcgcccacgc gcttttgtaa aacgggattg ccagccaaag
     3781 ctgcaccacg cttgccaatg cgaactgcca caacggcgga atcatccaat cgtgtctgcc
     3841 gatcatcatc cccgccatgc cgataaggaa cgggacgttg atggtgaaca gcagccacag
     3901 ccgccagccg atatggtgtt ctgcttcggg ttgcggcaat gtatcttccg ttttttcctt
     3961 cgcgccgtaa ccggttttct caatgatttt ggcaatgtcg gctactgagg ttttgctgtc
     4021 gtcaaacact acctgcgcct cttcgctggc gaagtttacc cccgccgatt cgacaaaatc
     4081 ttttttgttc aacacttttt caatgcgcga agcgcaggcc tggcaggtca tgccttcgat
     4141 ttggaaacgg attttttgtt gcataagcgt gattcctgct ttgatattca gacggcagtt
     4201 aaggctgcgg gatgccgtct gagcgccggt atggtcgaaa tttctcaaac gcgccgataa
     4261 ttcgagtaga atccgaaaac ttgacacagc aaaggaaaaa caatgtccca acaccgcaaa
     4321 ctgattattt tgggttccgg ccccgccgga tacaccgccg ccgtctatgc cgcgcgcgcc
     4381 aatttaaacc ccgtcattat tacaggtatc gcgcaaggcg ggcaactgat gacaacgacc
     4441 gaagtggaca actggcctgc cgatgccgac ggcgtgcaag ggccggaatt gatggcgcgg
     4501 tttctcgccc acgccgaacg tttcggaacg gaaattattt ttgaccaaat caacgccgtc
     4561 gatctgcaaa aacgcccgtt cacactcaaa ggcgatatgg gcgagtacac ttgtgatgcc
     4621 ctgattgtcg caacaggcgc gtccgccaaa tacctcggtt tgccgagtga agaagcgttt
     4681 gcagggaaag gcgtttccgc ttgtgccacc tgcgacggtt tcttctacaa aaaccaagat
     4741 gttgccgtag tcggcggcgg caatacggca gttgaggaag cactctacct tgccaatatc
     4801 gccaaaaccg ttacgctgat tcaccgccgc agcgagttcc gcgccgaaaa aatcatgatc
     4861 gacaaactga tgaaacgcgt agaagagggc aaaatcatcc tcaagctgga aatcaacctg
     4921 caagaagtct tgggcgacga tcggggcgta aatggcgcat tattaaaaaa caacgatggt
     4981 tctgagcaac aaattgccgt cagcggcatc tttatcgcca tcgggcacaa gccgaatacc
     5041 gatattttca aagggcagtt ggaaacggac gaagccggat acctgaaaac aaaaggtggt
     5101 acggcggaca atgtcggtgc aaccaatatc gaaggcgtat gggcggcggg cgacgtaaaa
     5161 gaccatacct accgtcaggc aattaccagc gcggcttccg gctgtcaggc ggctttggac
     5221 gcggaacgct ggctgggcag ccaaaacatt tgaatgccgt ctgaaaccga aattttcggc
     5281 ggaatgcaag tttttcgcac caaatccttg attccgtcgg caaaccccaa tataatgccc
     5341 gttctttgaa ccttgccttc tgctttcgca tggcagaagg ctgtttttac catccataag
     5401 gagataacat gcgtcattac gagatcgtgt ttatcgttca tcctgatcaa agcgagcaag
     5461 tgcccgctat ggttgaacgt tacaaaacca tgattaccga ggcaaacggt aagattcacc
     5521 gtctggaaga ttggggtcgc cgccaactgg cttacccaat taacaaaatc cataaagcac
     5581 attacgtttt gatgaacatc gaaactactc ccgaagttgt tgaggagttg gaaaccgcgt
     5641 tccgtttcaa tgatgcggta ttgcgtcatc tgaccatcaa aaccaaacac gccgttaccg
     5701 aagcatcccc tatgttgggt ggtgaaaagg ctaagaacct gctgagcggt gcgtctgaag
     5761 aagcggctgc ccaataattg ggattcaata atcttgtttc gcttgccgcg ttgattgaaa
     5821 aggctttccc tattcgatat acgcctgccg gaatccctgt tttagatatt attttaaagc
     5881 atgaatcatg gcagaaagaa aatggacagc aatgttttgt ccaattggaa attccggcac
     5941 ggattttagg caggcaggcg gaagagtggc agtatcggca aggtgtatat gttcacgtcg
     6001 aaggtttttt ggctcaaaaa agcagacgtt ccctgatgcc gatgctcaga atacaaaata
     6061 ttaaagaata taaaggttaa acgacaatgg ctcgtcaatc attcaaacgt agaaaattct
     6121 gccgtttcac ggctgaaaaa attcaagaag tcgattacaa acaagttgat ttgctgaaag
     6181 actttatctc tgaaaacggt aaaatcattc ctgcccgcat cacaggaacg aaggcattct
     6241 accaacgcca attggctgtt gccgtaaaac gcgcgcgctt cttggctctg ctgccttaca
     6301 ccgaccaaca caaataattt tggagattaa atcatgcaaa ttattctgtt agaaaaaatc
     6361 ggcggtctgg gcaacttggg cgacatcgta accgttaaaa acggttatgc ccgcaacttc
     6421 ttgattcccg caggtaaggc aaaacgtgct actgaagcga acatgaaaga gtttgaagca
     6481 cgccgtgccg aattggaagc caaacaggcc gaaattttgg cagatgcccg agcccgtcag
     6541 gaaaaattgg acggtcaaac cgttaccgtt gcacaaaaag ccggtgtgga cggccgcctg
     6601 ttcggttccg ttaccaatgc cgacattgct gctgcaatcg ttgctgccgg catcgaagcc
     6661 gtgaaagcaa atgtacgtct gccgaacggt cctctgaaag ccgttggcga gtacgaagtg
     6721 gaagtggctt tgcacactga tgccgttgct aaaattaccg ttgctgtcat tgccgcagcc
     6781 gagtaattga aattatcaga atgccgtctg aaaccatgcc gattatcgga atatggtttc
     6841 agacggcatt tgttttgttt cccgtatatt ctgcaaaggt cttggtcagg ttgtctgtcc
     6901 tcttgtcgga acaggagact gggcagcttt gtcctgatta attgcttgtg caaacggtaa
     6961 aaaggttttt tcgtgtgttt taaagcaggt tgtacagcat ttttccggca atcaaaagca
     7021 acataatgcc gaaggatttt ttgagtttgg cagaagaaag tttgtgggcg gttttgacac
     7081 cgagcggggc aaaggcaatg gttgccgcgc tgaggacggc gacggcgggc aggtaaagga
     7141 agcccagcga cccttcgggt aatcctgcaa tattcagacc gttgatcaga tacgatattg
     7201 cgccagagag tgcaatcggc caggcaaggc cggatgatgt gccgatggct ttatgggcgg
     7261 ggaagccgca gtggattaag aaagggacgg aaagtgaacc gccgcctatg ccgacccagc
     7321 tcgacattgc gccgaacagt gtggaaaccg cagtcagtcc gggcagtccg ggcagcgggc
     7381 gggatgccgt ctgacgaccg gtatgcagtg ttttgaatgc gacggcggtt aaaaacagga
     7441 tgaagaaaat ttgaagcccg aacgcgggga tatattttgc ggagagtgcg cccgtgaata
     7501 cgccgaatat catacccggc atcatcgcaa atacggtttt ccagtcgacc gcccgttttt
     7561 tgtgctgccc caacatactg gaaaaggcgg tgaagaccat gacggcgaag gatgtgccga
     7621 cggcgaggtg ttgcgcgtaa ggatgttgtg ccaaaccctg caaatcaagc acccataaaa
     7681 cgacagggac aatcagcgtg ccgccgccta cgccgaacag gccggcaata aaacctgccg
     7741 cactgcctac ggcaagcagg attaagataa tgtcccaatg ccacatttaa tccgcctttc
     7801 tgtatttttt ccaaaatcgg cgtgccgcca taatgtagcc ggacaggctg tatccgagaa
     7861 agaacaggaa gaggacgagc gacggttccc aagtgaccag aagcagcagt aagacggcaa
     7921 gcagcattcc gacaaagggg acttgtctgc ggatgttgat ttctttaaaa ctccaaaaag
     7981 ggatttggac aatcatcgac aggccggcaa acagtgtgat gcccaatgcc caccagtgga
     8041 cggcggggaa tttttcgacg ctgtggttga cccaaatcag cccgacaatc agcgcggcgg
     8101 cagtcggact gggtacgccg ataaaccagc gtttgtccac tttgccgatg agcgtgttga
     8161 acagggcgag gcgcagggcg gcgcaggcgc agtagatgaa ggcgacggaa taaccgattt
     8221 tgccgaactg ccaaagctgc catttgtagg caatcagagc gggagcaacg ccgaagctga
     8281 ccatatcggc aaggctgtcg agctgctccc cgaacgcgct ttggctgttg gtcagccgcg
     8341 ccacgcgccc gtccataccg tccagcaaca tagagatgaa taccgcgatg gcggcggttt
     8401 cataacgtcc gtgcatggat tgggtgattg cgtaaaacgc ggaaaacagc gcggcgatgg
     8461 taaaggagtt gggcagcagg tagatactgt tttgacgcag ggagcgttgc ggggtgttgg
     8521 tcggggtttc catagctgaa gtgtctcacg gacgtggaga atcgataggg gattataacc
     8581 caaaccatta tcgaacaata cgccctttag gatgaagccc tcggaggcgg tttgtttttc
     8641 tgatggcgga tgctttgcgg ggcggtttga ctctgcctgc caccgggtgt gttgtttcag
     8701 atttttgccg gatggatatt gttgccgggc atttctgggc ttgccggaag gtatcggggg
     8761 gagggcatcg gaatatgcgg cgcgccgttc aaaccggggc ggcgtttccc tattttcagg
     8821 gggttgcagg tttcaacaaa ttgaaagata ttttttgcat ttccgaccgt atgctgattt
     8881 tgaaacggga taaattatag gacaaggttt tgcgcccgcc gcaaaggttg gccgcaatgc
     8941 cgtctgaaaa caacggcttg ccgctaaggc ggcggtatgc ggatttgcgc tgccgaaatc
     9001 ggtttgccat acccggcggt gcaatctgcg gggagggata agccgataag gcgggactta
     9061 tcctgttttt tgtttataat gcaacagaat ttggacgaac cgtcgggaat ttatcatgat
     9121 gaaaaagata ctggccgtat cggcactatg cctgatgact gcggcggcac gggctgccga
     9181 tacttacggc tatctcgccg tttggcagaa tccgcagaat gcggacgatg ttttgcaggt
     9241 taaaaccaca aaagaagatt cgacgaaaag cgaagcgttt gccgagttgg aagccttctg
     9301 caaaggtcag gacacgcttg cgggcattgc cgaagacgag ccgaccggat gccggtcggt
     9361 cgtgtcgctg aacaatacct gtgtcgcgct ggcatacccg aaagccttgg gcgcgatgcg
     9421 cgttgaaaac gccgtcgtga ttacttctcc gcgttttacg agcgttcatc aggtcgcact
     9481 caaccagtgc atcaaaaaat acggcgtaca gggacaatgc ggcttggaaa cagtgtattg
     9541 cacatcttct tcttattacg gcggaactgt gcgctctttg attcaaaatc tcaaataaaa
     9601 cggaaaatgc cgtctgaaag atgttcagac ggcatttcta tatcgacggt caggattctt
     9661 tcggatcggg cagcaggctg ttcaacataa tggcaagtac ggcgcacaag cccacgccgg
     9721 caaagctgaa gctgcccaat ttgagcgtca tgccgccgat gcccgtggtc agtaccgagc
     9781 tgacgatgac caggtttttc ggcagcatca aatcgacttt ggcatcaatc agcgttttca
     9841 cgcccaaaga agcaatcgtg ccgaacagca gcagcataat gccgcccatt actggcatcg
     9901 gaatggaagc caaaaacgca ttgaatttgc cgaaaaacgc catgcagacg gcaaaaaccg
     9961 ccgcccaagt catgatgacg gggttgctgt ttttggtaat catcaccgca cccgttactt
    10021 cgccgtaggt cgtaaccggc gggccgccga tcagacccgc aacgcatacg cccaaaccgt
    10081 cgcctgcaag ggttttgtcc aagcccgggt ctttcgtata gtctttcccc gttacattgc
    10141 cgattgccat gatgccgccg atgtgctcga tggcgggggc gacggcaacg ggcagcataa
    10201 acagtgccgc ctgccagttg acctgaggtg tttcaaaatg gggaacggca aaccagggcg
    10261 cgtgtgcaat gcttgccgtg tccaccagtc ccatcagcag tgccaaaaca taacccgaag
    10321 cgacaccgat caagatggga atcagcttca tcatcctgct gccgaaaacc gatacgatgg
    10381 cggtaacggc aaaggtaaag ccggaaagaa tcagcgaatc ggtatagtcg atgacctgtt
    10441 tgccgtccgc ctgacccatt gccatgctgc ttgccgccac ggcaacagac agaccgatga
    10501 ccatgatgac ggggccgatg acgaccggag gcagcagttt gtgtaccgct gccagtccgc
    10561 gccaacggat cagcgcggca aacacaaaat acataaagcc ggcggcaaac agtccgaaca
    10621 tggtggaagg cagcccccat tcgccgacgg agtagataat cggtgcgata aaggcaaacg
    10681 aagaaccaag aaaaatcggc actttgcgtt tggttgtgat ttggaacagc agcgttccca
    10741 agcctgcgcc caaaagcgca agagccggat tcagaccggt cagcagggga accagcacca
    10801 ttgcgccgaa tgccacaaat aaaatctgcg cgccggaaac ggcaagtttc agttggttca
    10861 taccatcttc tttcaatcgg ttcaaacggc acggattata ctggtttgcc gagagtctgc
    10921 gtaaaaatac atatggcggg caacagatat gccgtctgaa aggcaatgtc tgtctgttgc
    10981 aggatgaaag caggagaaat ggagtataat ccgacacttt ccatattcaa tccgcaagcc
    11041 gtccggcttc cttatttcag acggcatttt ccggtaatac ggcatgacag aaaaatccca
    11101 taaaaaaaca gcaaaaggca gggcgggaag cccgtcccca acgtccgcac gcaacaagaa
    11161 agccgacaac ggcgcacggg gcaataaagt ttccgagcgg ctcaaggcgg tcaaagagtt
    11221 gcagaaaacc gaaaccaaaa aggcgcgtcc cgaacatgtc gtcaacctta tcggcgacgc
    11281 actgtggctg atgggtttgg cggcaacctt gtatttggcg atttccctga ttagtttcga
    11341 tatgggcgat ccgtcttggt cgcacagttc gccggttgtg gaagatgtcg ccaattgggg
    11401 cggactgttc ggcgcgtatg ttgccgatgt cggctattat cttttcggct ggtcgttctg
    11461 gtggtggata gcggctgcct gcgtcatgct gtataaaaat tttcgtctgc acgcaaaaca
    11521 gacggaaaac gaggcataca accacaaaat cgctgccgcc gcgctgtttg tcctgacggt
    11581 cttcagcccc gtcttggagt attttgtgct gggcggaaaa tatgccgact ccctgcctgt
    11641 cggagcaggc ggtatggtcg gcatacgcgt cggcgcagtg tttgcgtggc tgctggggaa
    11701 atcgggcagc ctgctgatta tcttggttgt tctgctgttg tcgttgtccc tgctggtgca
    11761 gatttcatgg ctggaatttt tgaacggtgc gggcagggcg gttcaaaacc gcctgagtgc
    11821 cttatccggc aaggtcatgg ctttgggaaa acgccggccg aataccaaaa cagacggtgt
    11881 cgatacccaa aatacacggc gcatggtaaa agaagccaag aatattacgg ccaaacccgt
    11941 tgccttgccc gaaggcagca gcagcaaccg caaatccgtc gcggtttccg tcgcgccgcc
    12001 gcccaaaatt caggtttctc tgtttgaaga tgacgaacct cggcaggcgg gcgaatacca
    12061 caagcctaca ttgaacctat tgcggattcc tgacagcgaa cccgtcagca tcaatcccgc
    12121 cgaattggag cgcactgccg aactgatcga atccaaactg gcagaattcg gcatcggcgt
    12181 acaagtcgta tccgccacat ccggccccgt catcacgcgc tacgaaatcg aacccgcgca
    12241 aggtgttaaa ggcagccaaa ttgttgcctt gtcgaaagat ttggcacgct ctatgtcgct
    12301 gcagtccgtg cgtatcgtcg aaaccatcgc aggtaaaaac acgatgggca tcgagttgcc
    12361 caacgacaaa cgccaagacg tgatgttgag cgaaatcttg tcctcgcccg tgtttgccgg
    12421 agccaaatcc aagctgaccg tcgcgctggg caaagacatt gccggtaccc ccgttgtcgg
    12481 cgacttggcg aaaatgccgc accttttggt cgccggtatg actggttcgg gcaagtccgt
    12541 cggcgtgaac ggcatgatta tgtctatgct tttcaaagct acgcccgacg aagtccgctt
    12601 tatcatgata gacccgaaaa tgcttgagtt gagcatttac gacggcattc cgcacctgct
    12661 ctgtcccgtc gtgaccgata tgcgcgaagc ggggcaggcg ttgaactggt gcgtcgccga
    12721 aatggaaaaa cgctaccgcc tgctttccca cgccggtgta cgcaatctgg aaggcttcaa
    12781 ccaaaaagtc gaagccgcaa aagcggcagg caagccgctg ctcaatccgt tcagtctgaa
    12841 ccccgacaac cccgagcctt tggaaaaact gccgatgatt gtggtcgtta tcgacgaact
    12901 tgccgacctg atgatgaccg aacgcaaagc cgtcgagcag caaatcgccc gtctcgccca
    12961 aaaagcgcgc gccgccggta tccatatgat tgtcgccacc caacgtccca gtgtcgatgt
    13021 cgttaccggc ctgattaaag ccaacatccc gacgcgtatg gcgtttaccg tgcaaagcaa
    13081 aatcgacagc cgtaccatcc tcgaccaaat gggcgcggac gaactgctca aatatggcga
    13141 ttcgctgttc ctccagcccg gcagtgccga accgactcgc ctgcaaggcg cgtttgtttc
    13201 agacgacgaa gtacatcaag tcgtcaacta cgtcaaatcg caagccccag ccgactatat
    13261 tgaaggtctg ctcagcggcg aggccgcgct ggaaactgcc aatatcgtta atccgaatgt
    13321 agacagcgac gaattgttcg atcaggcagt cgcctatgtt ttggaaagca aaaaaacctc
    13381 catttcgtct ttgcagcggc agttgcgtat cggctataac cgcgcggcaa acctgatgga
    13441 ggcactggaa aatgcgggtg tggtttcgcc gaccgatttg aacggcagcc gtaaaatttt
    13501 ggcgcacaag gaccatttat agcacccata tcgcaaaatg ccgtctgaac ggcggaattg
    13561 gcgtttcaga cggcatatta tgtttcaggc ggaacattgt gatatacttg ccagctaaaa
    13621 tctccccttt gcggcaatgc ggttcaaata tccgtaccgt tgcgctgttt gcttccccat
    13681 gcagggaaga aggtttacca ttttatcaac acaacaaatt taagggctta tgatgagcgt
    13741 aactgttgaa actttagaaa atctggaacg caaagtagtg ttgtccctgc cttggtccga
    13801 aatcaacgca gaaaccgata aaaaactgaa acaaacccag cgccgtgcaa aaatcgacgg
    13861 tttccgcccg ggtaaagcac ctttaaaaat gattgcccaa atgtacggtg caagtgcgca
    13921 aaacgatgtc atcaacgaaa tggtacaacg ccgtttctac gatgttgccg ttgcccaaga
    13981 attgaaagtt gcaggctatc cgcgtttcga aggcgttgaa gaacaagatg ataaagaatc
    14041 cctgaaaatc gctgcgattt tcgaagtatt ccctgaagtc gttatcggcg atttgtctgc
    14101 acaagaagtc gaaaaagtaa ccgcttccgt cggcgatgcc gaagtggatc aaaccgtaga
    14161 aatcctgcga aaacaacgca cccgcttcaa ccatgtcgaa cgcgaagccc gaaacggcga
    14221 ccgcgccatc atcgactttg aaggcaaaat cgacggcgaa ccttttgccg gcggcgcatc
    14281 caaaaactac gccttcgtat tgggcgcagg tcaaatgctg cctgaatttg aagccggcgt
    14341 agtcggcatg aaagctggcg aaagtaaaga cgttaccgtc aatttccctg aagactacca
    14401 cggtaaagac gttgccggta aaactgccgt gttcaccatt acgctgaaca acgtttccga
    14461 agcgactctg cctgaagtcg atgcagattt tgcaaaagcc ttgggtattg cggatggcga
    14521 cgttgccaaa atgcgcgaag aagtgcagaa aaacgtaagc cgcgaagtgg aacgccgcgt
    14581 aaacgaacaa accaaagaat ccgtaatgaa cgcgctgctc aaagccgtag agctgaaagc
    14641 acctgttgct ttggtaaatg aagaagccgc acgcttggca aacgaaatga aacaaaattt
    14701 tgttaaccaa ggtatggctg atgctgccaa cttggatctg cctttggata tgttcaaaga
    14761 acaagccgaa cgccgcgtgt ctttgggtct gattttggcc aaactggttg atgaaaacaa
    14821 actggaaccg actgaggagc aaatcaaagc cgttgtcgcc aacttcgcag aaagctacga
    14881 agatcctcaa gaagtaatcg actggtacta cgcagagcct tcccgcctgc aagccccgac
    14941 ttctttggcg atagaaagca acgtcgttga tttcgttttg ggtaaagcca aagtaaacga
    15001 aaaagctttg tcttttgacg aagtgatggg cgcgcaagtc tgattgttct gaaaatgccg
    15061 tcttagggca gtttttgaaa gcaccgaagc cgcttttaaa gcccgctttg gtgcttttcc
    15121 atcatgaaag gagacgaaat gtcttttgat aactatcttg tccctaccgt tatcgagcag
    15181 agcggtcgcg gtgagcgtgc attcgatatc tattcccggc ttttgaaaga gcgcatcgta
    15241 ttcttggtcg gcccggtaac cgacgagtcc gccaatctgg tggttgccca actgttgttt
    15301 ttggaaagtg agaatccgga taaggatatt ttcttctata ttaactcgcc aggcggttcg
    15361 gtaacggccg gtatgtcgat ttacgacacc atgaatttta tcaagcccga tgtatcgact
    15421 ttgtgcttgg ggcaggcagc aagtatgggc gcgttcttat tgtcggcagg cgaaaaaggc
    15481 aaacgtttcg ccctgcccaa cagccggatt atgattcacc agcctttaat cagcggcggc
    15541 ttgggcggtc aggcatccga tattgaaatt cacgcacgcg aactgttgaa aatcaaagaa
    15601 aaactcaacc gcctgatggc gaaacattgc ggccgcgatt tggcagattt ggagcgcgac
    15661 accgaccgtg ataatttcat gtccgccgaa gaggctaaag aatatggtct gattgatcaa
    15721 atcttggaga accgtgcttc tttacagttt taataagaaa tctgagagaa acaaatgccg
    15781 tctgaaaaca gttcaggacg gcatttgtct tatagggact gtatagggat gattttgttc
    15841 tggttgcgtt tggagccgtt ttcgccataa ttggttttaa catattcctc tacaatagct
    15901 aggaattccg ttgcaatgtt gtcgcctttc agtgtgactt tgcgctcccc gtccacataa
    15961 accggcgcga cgggtgtttc tcccgtaccg ggcaggctga tgccgatgtc ggccaatttg
    16021 ctttctccgg gcccattgac aacgcagccc attacggcaa cgttcaggga ttcaacccca
    16081 ggataaaggg tacgccatat agacattttt tggcgcaggt aattttgaac atcttgtgcc
    16141 agctcttgaa atacggtact ggtggtacgc ccgcagccgg ggcaggcggt aaccatcggc
    16201 gtaaacgaac gcaatcccat agtttgtaaa atctcttgcc cgacgacgac ctcctgagta
    16261 cgcgggctgc caggttccgg agtcagtgaa atgcggatgg tgtcgccgat tccttcttga
    16321 agcaagacgg ataatgccgc cgttgatgcg acaatgcctt tgctgcccat accggcttcg
    16381 gtcaaaccca aatgcagcgg ataggcgcaa cggctgccca gttcgcggta aacctgaatc
    16441 aaatcctgaa ccgcgctgac tttgcacgac aggatgattt tgtcttcggg cagtcccaat
    16501 agaacggctt tttcggcaga ttccaaagcg gagacaatca gtgcttcctt cgtcacttct
    16561 tcgggcggtt tcggcgcgga agaagcgagg ttggcatcca tcatacgttt ggcgaggctt
    16621 tgatccaaag aaccccagtt tacgccgatg cggacggctt tatcgttttc agcagcggtc
    16681 cgaatcataa aggcaaattt ttcatcgcct tttacgcctt tgccgacatt gccgggattg
    16741 atgcggtatt tggacaatgc tttgccgcat tcgggaaatt ccgccaacag gcgttcgccg
    16801 ttgaagtgga aatcgccgat aagtggtgtg gcatagccca tatcgtccaa gcggcggcgg
    16861 atttcggcaa ctttggacgc ggcttcgggg ctgttgacgg taatacgcac catttcggat
    16921 ccggcatcgc tcaattcctt aatctgcaat gcggtggctt ttgcatcggc agtgtcggtg
    16981 ttggtcatag attggataac gacgggtgct tctgaaccga cggtaatatg atcgatgcgg
    17041 acttgatgcg tcttgcggcg ttggagtgtg ttcatatggt ttgaatctgt ttattgaccg
    17101 atgaggacgg tttgcaattc ttccgagtag gggaaattcg cctgcaattg tgcttcatat
    17161 tcgtatgccg cctgtgcgtt gccgagggct ttggcaattt tccagcctag cagcaaatca
    17221 tcggcctgaa ggacttctac cctgctttgg tattttttaa agtagtaatc ggcatcgccc
    17281 aactgcccgg ccagcatttt ggtgcgcgcc agttctttaa atgcgggtgg gaactgcggc
    17341 tgggcggcga gggaacgttt caaataggct tccgccaatc cgaattgccc ctgttttgcg
    17401 ctgcatatgc ctttattcag gttggcaata taaggggtcg ggtaggtggg gtcggccagg
    17461 gctttgtcga aatatgccat agattcggca gggcggttga gcctgccgca caggaaccag
    17521 ccgtagttgt tgttgatttc ggcactgtcg ggtttgatgg agagggcttg ccggaaactt
    17581 tcctgcgcct tgtcgttaac tttcaggtat tgatagattt cggcacggac cagccaggca
    17641 agctcgtttt tagggtctga tttcaaggca tcttcaatac ttgccgtcgc ctgacggtac
    17701 tcctgaccgc gcatatattc cattgccaac tgggttttga tattggaaac ctgattggct
    17761 ttttctgccc gcgaggggcg gtaggaagtg ctgcacgcgc ccaaggcaag aacaagtaat
    17821 aaagagattc gtttggatgg cttaaaaggc ataattaccc ctgttgtccg attaaaatct
    17881 gctgccattt ttgttggcgg cgcgttttat cctgaacctg ccccgccaac tgtccgcagg
    17941 cggcatcgat gtcgtcgccg cgcgtttttc gtacggtaac gacaaatcct gcctgctgca
    18001 aaatatcgcg gaacacacgg atgttctcat tgctggagcg ttcgtatccg gagtttggga
    18061 agggattgaa cggaatcaga ttgaacttgc agggaacatc tgtgaccagt tcgatcagtt
    18121 cgcgcgcatg ttgcgcctta tcgtttattc cgtccaacat gacgtattcg aaagtgatga
    18181 aatccctggg tgctttgact agatagcgtt ggcatgcggc catcaattct ttcaagggat
    18241 attttttgtt caacggtacg atttggttgc ggacttcgtc attggaagcg tggagggaaa
    18301 ccgccaaagc caccggcatg acatcgcgca acctgtccat ttggggaacc atacccgaag
    18361 tggaaacggt tacgcggcgg cggctcaaac cgtagccgtg gtcgtccagc atgatgctta
    18421 aggcggtaac gacattgtcg aagttcgcca tcggctcgcc catgcccatc atgacgacgt
    18481 tggaaataac gcgctcgttt ttcggtgtaa cgcccatcgc tttgtttgcc caccacaatt
    18541 gcccgatgat ttcggcagag gtcaggttgc ggttgaagcc ctgccggccg gtcgaacaaa
    18601 atgtacattc caaagcgcag ccgacttgtg aggaaatgca gagcgtgccg cgatccgatt
    18661 cggggatgaa gacggtttcc acgccgttgc ccgtaccgac atccaaaagc cattttcgag
    18721 tgccgtctga agatttttga gacatcatca gcttgggaat ttcgattcct gcctgttcgt
    18781 tcagtttatg gcgcaacgat tttgccaaat cggtcatttc gttaaaattt tgcgcgccgg
    18841 attggtgcat ccaacgcata acctgtttgg cacggaaggg tttttcgccc atatcggcaa
    18901 aatgtcgggt cagcccttga aggtcgtagt tgagcagatt ggttttcatg tgtttgtgtt
    18961 tttcttaaat taaggcttca aataaaaaat tgcagggcag gcaagtttga tttgaagcct
    19021 tttgcgacag agttttcaga cggcatggat atatttatgc cgtctgaaga ggagttgtgc
    19081 ggtgtattgt atcaacgggg gcagatttcg gtttggctga aaaagtaggc aatttccaaa
    19141 gcggcatttt ccacgctgtc ggaaccgtgt acggcattaa tgctgaccga agtggcaaag
    19201 tccgcgcgta tcgtgccttc ggcggcttcg gaaggattag ttgcacccat cagttcgcgg
    19261 tttttcagga cggcgttttc accctctaat acctgaatca taaccggacc gccggtcata
    19321 aattcaacca atccggcgta gaaggggcgg tctttatgaa ccgcataaaa ttcttgcgcc
    19381 tctttgagag taagctgctt cattttggcg gcaacgattt tcagaccgtt ctcctcaaag
    19441 cggctgtata ttttgccgat aacgtttttg ccgacggcat cgggtttgat gatggagatg
    19501 gtgcgttcaa tcgccatgct atatccttat ttttacttaa gaagaatcaa atcggtattc
    19561 tatcaaaaaa ataacttatc cgcctgaagc cttgggagat agggtcaggt gcggctgttc
    19621 caaataaact tcggactgca tttcgaccat ccggctggcg gtgcgggtaa attcttcggc
    19681 aaaatcgcct tccgtataga tatggttgac atctaccgcg ccggtggcac acagtttgac
    19741 ccggaaatcg tagagtacgt caatcagcca agtcagccgc cgcgcctccg ccttttcttg
    19801 cggtgagagt tgttccaaac ctgaaataaa aaccatttca taatgttcgg ccaaatacag
    19861 atagtcggac tgtgagcggg gaccgaagca cagtgcgcgg aaatcaaacc atatggcacg
    19921 gccggactcg gctttgtggg gaatctcccg accgtggatg gtgctgatgc cggggttcaa
    19981 atcggtaatg cctgtcattt ctttgaacag ttttgccagt tttgcctcat tttcttcatt
    20041 ggcaggcgta aagaaaatct cggcggggcg gagggtacgc agtcggtagt cttcaccgcc
    20101 gtcaacgttt aagacggtca ggctggactc gatgagcgcg attgtgggaa gaaaactgct
    20161 ccggttttga ccttgcgggt agagttcgga aggcgcgtag tttgaagtcg ccaccaaaac
    20221 aacgccctcg ttaagcaggt tttccagcag acggcctaaa atcattgcat ccgcaatatc
    20281 gctgacatga aattcgtcaa aacacaatac gcgggtttct ttggcaatct cggcggcaac
    20341 ggatttcaac gggttgcttt cgcttttcag ggttttcagc ctctggtgga tttctgccat
    20401 aaatgcatga aagtggacgc ggcgtttgcg gcggtaaggg aggcagccga aaaaagcgtc
    20461 catcagaaag cttttgccgc gtccgacccc gccatagaaa taaagccctt tggggacttg
    20521 cggggaacgc aaactcctgc ctaaaaaacg gtttcttttg cgtttgaaca tcatcaattc
    20581 ggtccaaagc cgatcgaggt gttcgatggc ggctgcctgc gcgtcgtcgc ggatgaagtt
    20641 gggcagttgt gaggcagcct gataccaggt cagcgggctg tggttttcaa acggcggggc
    20701 tttaaaaagt tggtctctat tactcatatt taaccttgtt atacttcttg cggcggagaa
    20761 agaaaatgct taccgtcggt attataaatg atttccttca aacttggcat tttccgtgga
    20821 aaagaataat gacgcgcaca agggggtata agcaaaggcg gcgtttcaat atggtgcaga
    20881 tatgagctca aatgcctttt atagt
//
LOCUS       DAXHRI010000019        20704 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXHRI010000019 DAXHRI010000000
VERSION     DAXHRI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.019"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            1..99
                     /locus_tag="ACFM75_000544"
                     /pseudo
     CDS             1..99
                     /locus_tag="ACFM75_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(120..1034)
                     /locus_tag="ACFM75_000545"
     CDS             complement(120..1034)
                     /locus_tag="ACFM75_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6255136.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="ACFM75_000546"
     CDS             1290..2759
                     /locus_tag="ACFM75_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC6255137.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="ACFM75_000547"
     CDS             3011..3382
                     /locus_tag="ACFM75_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255138.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /gene="thrC"
                     /locus_tag="ACFM75_000548"
     CDS             3455..4864
                     /gene="thrC"
                     /locus_tag="ACFM75_000548"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC6255139.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGREALD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYGQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="ACFM75_000549"
     CDS             4910..5704
                     /locus_tag="ACFM75_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255140.1"
                     /translation="MTVPMQETHFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="ACFM75_000550"
     CDS             5892..6668
                     /locus_tag="ACFM75_000550"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6255141.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7335..9800)
                     /locus_tag="ACFM75_000551"
     CDS             complement(7335..9800)
                     /locus_tag="ACFM75_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6255142.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADARKTELPPQEILDQIRLYDLPEVQSDFVETHGGTHEAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGASTVDESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRADYVAEIAGKPPQDGQASESGSAV
                     FLGSQSGFAAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAH
                     YRAQAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGAD
                     IVLLNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMS
                     FSSLAVLGNTLRLHKREEMPSEQ"
     gene            complement(9797..10975)
                     /gene="hflX"
                     /locus_tag="ACFM75_000552"
     CDS             complement(9797..10975)
                     /gene="hflX"
                     /locus_tag="ACFM75_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC6255143.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELM
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KIDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            complement(10980..12260)
                     /locus_tag="ACFM75_000553"
     CDS             complement(10980..12260)
                     /locus_tag="ACFM75_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC6255144.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGATFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            12262..12390
                     /locus_tag="ACFM75_000554"
     CDS             12262..12390
                     /locus_tag="ACFM75_000554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255145.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDAV"
     gene            complement(12413..13138)
                     /locus_tag="ACFM75_000555"
     CDS             complement(12413..13138)
                     /locus_tag="ACFM75_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC6255146.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13211..13888)
                     /gene="radC"
                     /locus_tag="ACFM75_000556"
     CDS             complement(13211..13888)
                     /gene="radC"
                     /locus_tag="ACFM75_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC6255147.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQSVCSF
                     RQLGLMP"
     gene            complement(14011..15360)
                     /gene="gshA"
                     /locus_tag="ACFM75_000557"
     CDS             complement(14011..15360)
                     /gene="gshA"
                     /locus_tag="ACFM75_000557"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC6255148.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPFLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDALGMPRSEKDCDVDNVHNRLYVYGVMARLSLLAASIELEETA"
     gene            15620..17029
                     /gene="leuC"
                     /locus_tag="ACFM75_000558"
     CDS             15620..17029
                     /gene="leuC"
                     /locus_tag="ACFM75_000558"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC6255149.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFKAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17126..17380
                     /locus_tag="ACFM75_000559"
     CDS             17126..17380
                     /locus_tag="ACFM75_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255150.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17442..18083
                     /gene="leuD"
                     /locus_tag="ACFM75_000560"
     CDS             17442..18083
                     /gene="leuD"
                     /locus_tag="ACFM75_000560"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC6255151.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18148..18609
                     /locus_tag="ACFM75_000561"
     CDS             18148..18609
                     /locus_tag="ACFM75_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFC6255152.1"
                     /translation="MITDTISNATRYAALHPDFAEAFHLLQTLDFAKLPDGQVPCKNP
                     NIRIFIGSEPMRTKGEAKPEAHLKHIDIQTPIDGSETYGWIDRGRLKNGLGYNEKRDI
                     EFFNCEPETWLTLEPGEFALFFPNDAHAPLVGDKANIRKAVFKIRIETERL"
     gene            18703..19773
                     /gene="leuB"
                     /locus_tag="ACFM75_000562"
     CDS             18703..19773
                     /gene="leuB"
                     /locus_tag="ACFM75_000562"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC6255153.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAILYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            19936..20499
                     /locus_tag="ACFM75_000563"
     CDS             19936..20499
                     /locus_tag="ACFM75_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC6255154.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         4808 a         5641 c         5455 g         4800 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggttg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggctccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgggaagcc ttggacaaat ggcgcggttt
     3601 ggcttatccc gaattggcgt ttgaaatcat gcgcttgttc gttacggata ttccggagga
     3661 cgatttgcgc gatattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggt aaaaaactca atatcttggg cgcgaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggcgt gaacgtattt atgttgtcac ccgacggtaa
     3961 gatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacggaaata tccacaatat
     4021 cgccgtgaag gggatgtttg acgattgtca ggacattgtg aaggcagtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac agtcaactcg attaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcgggcta tttcaaggcg acgcaaagca atgacgagca
     4201 ggtcagcttc tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac acatcgccaa
     4261 acaaatgggc ctgcctgtcc gccgcctgat tgtcgcgacc aatgaaaacg atgtattgga
     4321 cgagtttttc aaaaccggcg cgtaccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtcg atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtagcggcgg gcaagggctt
     4501 tgacctgcga tttgccttgg acaaagtcgg cggcaaatac ggctttactt ccggcaagtc
     4561 cacccacgcc gaccgcctcg ccactatcaa gcaggtttac gggcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggtgaaac
     4681 ggtggtttgt ttggaaaccg cgttggcggc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgtc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcaacg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccgtacc
     4921 tatgcaggaa acccattttt ccatactgct ggacgaactg gcggcaaaac aggaggcagc
     4981 catcgccccc cacctgcttg ccgacggaac caaggtatgg atacgcaaag caggcaggca
     5041 caatgcgcga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgcgcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggaaatttgg aaggtatccc gctcgatacg caaatccgcc aagaagccga
     5281 agccggaaag gcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcacg aaatatgatg tcggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg acccttccga ggtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacattc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtatt gtcagatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 gtaagccgcc gcccgccccg cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta ccccgaatcg
     5821 ggcagacatt ttcactctgc cgcacatccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgcgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggtccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaaccctggt tgccggcgac ctgaatccgg gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc tcctttcttg agcatcacca aagaccccga aatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gtgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcgcgcgaa gaattcgaac accacggtcg cctaaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa cccgcaagac gaccgcgcca
     6541 tgctgtgcgg cagccccgcg atgctgaaag acacctgcaa agttttggac gatttcggtc
     6601 tgaccgtctc cccgaaaaca ggcgtgcgcg gcgactacct gattgagcgt gcgtttgtag
     6661 atcaataagc tgtttgaaaa tcccgtctga acggcatcag cgataaaaaa acccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg ggtaaaaatt agaaatgcac atttccgtca
     6781 ttccaacaca ggtgggaatc cggacttacc tgtttcatta tcgtttgaaa ataaaagaga
     6841 aaccaaacat atagattccc gcttgcgcgg gaatgacgac ggtgttttgc cgtagccaaa
     6901 gcagaaaaac atccctattg tcatttcatc atgatatgac aataggatgt taaaaattaa
     6961 ttatcattat atgattgata tataagaaaa actgtcccaa taagaaatgt tatgcagaaa
     7021 ataaaatgaa aaggatggtt ttcattgaaa atggttgttc tgccaccgtc atacacagag
     7081 ccagtatttg gaaaaaaatg atccatattg acatcattat tccatttgtc gcataaatcc
     7141 actttttaat tttttccaca ttaatataat ctcttattta ataaaatcaa aataaaaatc
     7201 aaatgcctaa caattcgatc tcaaattttg aacgccaacg tgtttgagcg gtaacaatac
     7261 atactttaag tttgcaatcc ttcatcgtct tatttttgtt ttcatcaact atacatctga
     7321 agcagcgccg tccgtcattg ttcggacggc atttcctccc gtttgtgaag gcgcagggtg
     7381 ttgcccaaaa ccgccagcga actgaagctc atacccagtg cggctatcca cggttggaca
     7441 tagcccaaaa cggcaagcgg tacggcaatg atattgtacg cgcccgccca tatcaggttt
     7501 tgccggataa tatggcgggt gcgccgcgcc tgatcgagca ggtgggcgac ggtacgcaaa
     7561 tcttcgttca ataacacaat gtccgcgccg tccctcgcaa tatccgtccc gcccgctgcg
     7621 gcggcggata cgtctgcctg cgccaaaacg ggcgcgtcgt tgatgccgtc gcctatcatc
     7681 agcacttttt tcccttcttt ttgcaaggct ttgacgtatt ccagtttgtc ctcgggcatg
     7741 gcttgggcgc ggtagtgcgc gacacccagg gcgcgtgcgg tttcggcaac ggcggtttcg
     7801 cggtcgccgc tgaggatgtg cagggtcagg tttttgcctg ccaactgccg caccgcctcc
     7861 gccgcgctgt ctttcaaggg gtcggtcagg tagaacacgg cggcaaaacc gctttggctg
     7921 ccgaggaaaa ccgcgctgcc gctttccgaa gcctgtccgt cttgcggagg tttgccggcg
     7981 atttcggcga cataatccgc cctgcccaac gcccaaactt gtgtttcgcc gttgacggtc
     8041 agttgcgcgc ccacgccttc gccgatgcgg ttgaggcgtt gtttaatagc aatgtcgggg
     8101 acgctgccgc ctgaaatgcg gcagttgagg atggcgcgcg cgagcgggtg ttcggactgc
     8161 tgttccaaac tttgcgccac tgccagcact tgggcttcgg tcatgctgcc gagcagttca
     8221 atccggcgga cggcgggatt gccttgggtc agcgtgcctg ttttgtcgaa aatgatgtcg
     8281 gtggtttggg cgagggtttc gattgcctgc tttccgccga ttaaaatacc ttcgcgcgcc
     8341 agcgtaccgg tagaagctgc cagcgcggtc ggcgtggcaa gcgataaggc gcaggggcag
     8401 gtaatgacca gcagggcgac ggtaatccac aatgcggtgt gcgcgtcggc gtacagcgtc
     8461 cagccgatga agactgggac ggcaagcagg agttcgccga atatgaaaga cgaggcatat
     8521 tgttccgcca actcggcggt gcgcggtttt tgcgccaagg cgcggtcgag caggcggacg
     8581 atgtgcgaca gtcgcgtgcc gccgccggtg cggtcggtgc gtataatcag ggggctttgc
     8641 gtgttgagtg tacctgcggt tactttttca gacggcattt tggcgacggg caggctctcg
     8701 ccggtcagca tagattcgtc aaccgtgctt gcgccttcca gcaccgtgcc gtcaacgggg
     8761 atggtttcgc ccggtttgac cagcacgata tcgcccgcct tcaatttgac gacagctgcc
     8821 tcgcaggttt cctgcgtatc ggggtaatcg ggcatatgat ggcaaaacgc aggaatcagc
     8881 ttcaccagcc tttcggcggc atcgcctgcc ttgcgtcggg caatgtgttc cataaagcgt
     8941 ccgcccagca ggaaaaacag cagcatggct atggattcga aatacatccc ctgccccgca
     9001 ttggtcgcaa ggctgtaaac gccggcgata aaggtcatga tgatggcgac ggcaatcggc
     9061 gtatccatgc cggcgcggcg gtttttcagg tcgcgcaacg cgccttgata aaacgggacg
     9121 gcgcaataga atacgacggg cagcaccatt aaaaagccac cccaatggag gatttgcagg
     9181 aaatcgggtt cgatgtcgcc gccgtaaagg taggtcggca gcgcgaacat catcgtctgc
     9241 atcatcccca gcccggcaac ggcgaggcgg acgatgtatt gtttgcgttc tttttggttg
     9301 gcggcttcga ttttttgcgc gtcatagggt gcggcggtgt agcctatctg cctgattttc
     9361 aacagaatgt cggaaaggcg gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg
     9421 ctgtaattga ggtcgatgcg gacgatgccg tctgtacgca aaagctgctg ttcgatcagc
     9481 cagacgcagg cggcgcaggt aatgccgccg agcattaaaa ccgcttcgtg cgtgccgccg
     9541 tgggtttcca caaagtcgga ctggacttcg ggcaggtcgt acagacggat ttggtcgaga
     9601 atttcttggg gcggcagttc ggtttttcgc gcgtcggcgg tgcgttgttt gtaataactg
     9661 cccaagcccg cgtcaataat gctttgtgcg actgcctgac aaccggcgca gcaggtttcg
     9721 cggtcttcat tttcgtaacg gacagtcagg tggaggtgtt cgggaacatc cagaccgcag
     9781 tggaaacagg tttttttcat ggcatttcgg tttcgtctgt gtttggtgcg gcggcacaat
     9841 actcggcaat ggcttcgcgc agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga
     9901 cggcggcaat ttttcccgca gcgtcgcgcc atatgcccgt gttttgttct tcagacggca
     9961 gcaggtcgat tttgttgtac accttgatgc acggaatatc gccggcatgg atttcccgca
    10021 gtacgttttc cacgtcttca atctgctgtc cgctgttcgg ggcggcggca tcgacgacgt
    10081 gcagcagcac atcggcttgc gcggtttctt ccagcgtggc ggaaaatgcc gaaatcagtt
    10141 tgtgcggcaa atcactgacg aatccgacgg tatcggtcag gataatgctg cattcgggac
    10201 tgatgtacag ccgccgcgcc gtggtgtcga gtgtggcgaa aagctggtct ttcgcatata
    10261 tgcccgactt ggtcagccgg ttgaacaggc tggatttgcc gacattggta tagccgacca
    10321 gcgcaaacgt tttgattgtg cccgattcgc gggacttgcg gcgcagggcg cgctgttttt
    10381 tgaggttggc aagctgtttt ttcaaggcat tgatccgatg ggcgatcaat cggcggtcgg
    10441 tttccagttt ggtttcgccg gggcctttca tgccgatacc gccgcgctgg ctctgcagat
    10501 ggccgtaacc gcgtatcaag cgtcccgcca aatggctcaa ttgcgccaac tcgacttgca
    10561 gcctgccttc ctgcgtgcgg gcgcggcgag cgaaaatcgc cagaatcagc cctaccctgt
    10621 ccaatacgcg gcatttcagt tctttttcaa ggttgcgttc ctgcgtgggc atgagttcgt
    10681 ggttgaatac gaccaaatcg atgccgtctg cggcaactgc ttccgacagc tccgccgcct
    10741 tgcccgtgcc gacaaacagc gcggtgtgcg gacggtcgcg tttggcagtc tccacgcgca
    10801 cggaatcgcc gcccgccgct ttgaccagct cgacagcttc cgccaatgcc gtctgaaaac
    10861 cgttcagacg ggcggcacta ctgcccgtac catctttgtc caacattacg cccaccagca
    10921 tgacgcgttc gggttgcgct tgggtactgc cgttgcggcc tgttctgcct gtcatgtatt
    10981 caatcctgta aataaagggt tacgcctcgt tccgtttctc cttccggtgc aagcgtgatg
    11041 cggctgcccg ccattccgtc aacgctgaag acgatgccgc ccgcctcgtg ccttcctatg
    11101 gacaggcggc tgccgggtcg ctgccggtag gtataacggg tacacgcttc caaacaccag
    11161 cgtccgcgcc agtggatgaa atatttttcg ccttttctaa ccgcaagtgt ctcgccgccg
    11221 tttacctcaa aataagggta atcgggtttt cgggttctaa actgccattt cgcctgcgcg
    11281 cgccgtccgt tgcggacata gtcgaatacc gccgtataaa gcgtaccgta ttccaaaggc
    11341 ttgagcggga aaagcgcgaa ttggtacgcg gtcagcctgc cgttggggtc gttgccggcg
    11401 gttaaaaccc tgacggggcg gatttcgttt ttaccctgat acagcttgaa acttttcatc
    11461 gtaattttgc ctgccgcctc ggaaaaatca atgctggcag gattgcccgt gatttcatat
    11521 tccggcacgg ggtctggacg ctcgccgtgg aaataaggca gtgcgccgtt gccgacggga
    11581 taggctgtat agagcagctc ctgtgcgggc atggcttcgt cggtgtacac gaccgcaccg
    11641 ttatggcagg cgttgcggta atatttccgt cctgcttccg gctgatttct gccttgggcg
    11701 caatgccgct caaacctgcc gttgccctga ttgaatacga gaacggtttt accgttttcg
    11761 cgcacaaatg tcgctcctgc ctcatccgta tggcggtcaa gtagggaaag gcggtggtag
    11821 attgcgctca ttaatccgtc cacttggcgt tgctgcgtgc ggatgtcgct gtcggacgat
    11881 tcggcggctt cctcttccgt gctgatgttt tcatgcacgc cgttgtagag atacccggca
    11941 aggcgtgtgc gttcggtcag cttttgtgcg gtgtagtgcg gattgtcggg atggtgttcg
    12001 ccgtgtccgt cttcgggatt gagcgtgaga tagcgtgcgt gcctgcgggc ggaattttcc
    12061 aaaaccggcg cgtgtgccag cttgtgcaaa ccgatttggg cgcggatgcg gttgaggtag
    12121 tgcagcgcat cgccgccgtc cctgatttgc ggtgcggacg gatagacaag ttcgcccgcg
    12181 ggcagggatt ggttttgggt atgatagaaa acgcctgccg ccgaacccaa caggagcagc
    12241 cgaataaaaa gggatttcat aatgccgtcc tcccgacgcg gcaacggggt gttttatcaa
    12301 aacggcaaac ttgccaatgc ggtttccgat tgcagattgc caaaccggca aacctttccc
    12361 cgtgccgatg ccgaacctga tgccgtctga accttcagac ggcatcggat gtttatttgt
    12421 ccacccggat gggtgcaggt tcgtattgtg tcgattcgtc gccgtaatac agcacgccga
    12481 gtttgatggg gattctgccc tgtgatttgc ggtgggcatt ggaatccctc agggaatagg
    12541 cacaaccgca atattcctgc tggtagaagt tttcacgttt gctgatttca atcatgcgcg
    12601 cgctgccgcc gcctttgcgc cagttgaaat cccaatacac cacatcatcg taaggcgcgg
    12661 cggcgcggtg tccgcagtcg ttgatttgcg ccatattttt ccagcgtgaa atgcccagcg
    12721 aactggtaaa gacggggaac ccgtgttcat gcgcgtattg cgccgccttt tcaaaacgca
    12781 tatcgaaaca catggtgcag cggatgccgc gttccggctc aaactccatg cctttggctt
    12841 tggcaaacca ttctttgcgg tcgttttcgt agtcgtcgtc tttatcgatg aaaggaatgc
    12901 cgaacttttc cgcaaagcgc acgttttcct cttttcggag catatactct ttgtgcggat
    12961 ggatattggg attgtaaaaa taaatggtgt agtcgatgcc gctggccagc atggcttcca
    13021 tcacttcgcc gctgcacggg gcgcagcagg aatgcagcaa gactttttta tgtccaccgg
    13081 gcgggacgag tatagggcgg tcaatgtcgg taacggtagg tttgttttgt gtttccatta
    13141 acttaacaat atctgttgaa tacggttgaa gcggctattt tatcagagcc gccgcatgta
    13201 aaacagagtg tcagggcatc aacccgagct gtctgaacga acagacgctt tgcgaggtaa
    13261 cgataaaatg gtcgagcagc gacacatcga ccagcgacat tgcctgtgcc agccgccttg
    13321 tgaacatgat gtcttcctgc gaaggttcgg gcgagccgcc cggatggttg tgcgcaataa
    13381 tcaggctgtc ggcatattcg tccaatgcca gtttgacgat ttcgcggatg taaatcgtgt
    13441 tttccgcaac cgtaccgcgc gacagctctc tgaccgcaat cagttggttt tggcggttca
    13501 gcagcagcgc gacgctgact tcgacttttt cctgccccaa atgaaagcgt aaataatcgg
    13561 ccaccgtatc cggatcggac aggacgatgc tctcctgcaa ttcttccgcc aatatccgcc
    13621 gcccgatttc cctgaccacg gcaaactgtg tgaaacttgc cgtccccatc cctttgtatg
    13681 ccgacagttt gccgacctcc gcgctcatca gcctccccaa actgccgaac tcctgcagca
    13741 aataacgcgc caaatcaacc gcactcattc cgcgcgtgcc gacgcgcagc aggattgcca
    13801 aaagttcggc atcactcaac gccgccgccc cgcgttccaa cagcttttcc ctgggccttt
    13861 ccccctccgg ccattgcttg atgctcatgt tttccctttt ttaaatagtc tgagatgccg
    13921 tctggaacaa atgttcccac acgccaaata tccgaaatgc cgtctgaacc ggatttccgc
    13981 ttcagacggc atctgtattt caaaacagtc ttacgccgtt tcttccaact cgattgaagc
    14041 cgccagaagc gacaggcgtg ccatcacgcc gtacacatag aggcggttgt ggacattatc
    14101 gacatcgcag tctttttcag aacgcggcat acccagcgca tcccattgct caaatacgcg
    14161 cttgcaggct tcgggtgctt cttgggaatt atcgccattg ccggtaggaa tgctattgga
    14221 cagcggcaca aacaccatac cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg
    14281 tacgcggaaa aagccgccga tgacgaagcg gtccatcata tacacgacgg gttcgcacac
    14341 cgcgccgttt aaggtttcat aagtataaat cccttcttgg acaatcactt cgctgacttc
    14401 caagccttct ttgactttcg ccattttatt gcggttttta cggttcaatc cgcgcacttc
    14461 gtcggcggat ttgacgctca tcacgcccat gccgtaagtg ccggcatcgg ctttgacgat
    14521 gacgaaaggt ttgtcggtaa tgcccgattc gtcgtatttg gcttgaattt tcgccagcac
    14581 acgttctacc gcttccgcca acgcgtcttc gccttcacgc ccttggaagt ccaaaccgcc
    14641 gattttttca aaataagggt taatttgcca ttcgtcgatg ccgattaact tggcaaattc
    14701 ggcggtaact tggttgtacg cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc
    14761 gtgcaacggc ggcaaaacgg tttggctgat gcctttgagg atgtcgggaa tgccggcaga
    14821 caaatcgttg ttcaacaaaa ccacgcaagg cgaaaagccg tctgcaagat ggacgcggtc
    14881 gcgggtacgc aagaaaggtt ccaacaggat tttgtcgccc aatgcagttt caaattcggt
    14941 cggttcggtt acttccggat tcaggctgcc caagcgcact tcataccctg ccgaacgcaa
    15001 aatctcgccg agggcgtaaa tgttttgcag gtaaaacgta ttgcgcgtgt ggttttcggg
    15061 aataatcaat acggatttcg ccgtttcgca ggcgcgttgc accgcatctt gcgcggcaac
    15121 cgccgccagc gggataaagt tgggattcag attattgaag ccgccgggga acaaattcat
    15181 atcgatagac gaaattttgt aaccggcatt gcgtatatcg accgaaccgt aaaacggcgg
    15241 gcggtgtacg ctccactgcg tgcggaacca cgcctcgatt ttggcgtgat tggacaggat
    15301 ttttgcctca aacgcctgaa gttgcgccga atgttcgggc gacatcaccg gtaatttcat
    15361 ctttcgtctg cctctgtgtg ttttttgaca agatgcagat gataatgcct ttgaaagaat
    15421 gacgacaagt attctttcat atcgaatcat gctttagata taactgttta ctgaaatgga
    15481 caatatgtct aattttttcg ttaaaattgt ttgcaaaata acaccggctc aaaaattaga
    15541 caaaatttgt tgcgcggtat aaagaatacg tctaaaatcc gcccaatccg cattaccatt
    15601 tatccaaaag aacaacatca tgacagcaca aaccctctac gacaaacttt ggaacagcca
    15661 cgtcgtccgc gaagaagaag acggcaccgt cctgctctac atcgaccgcc atttggtgca
    15721 cgaagttacc agccctcagg catttgaagg cttgaaaatg gcggggcgca agctgtggcg
    15781 catcgacagc gtcgtctcca ccgccgacca caacaccccg accggcgatt gggacaaagg
    15841 catccaagac ccgatttcca agctgcaagt cgatactttg gacaaaaaca ttaaagagtt
    15901 tggcgcactc gcctattttc cgtttatgga caaaggtcag ggcatcgtac acgttatggg
    15961 ccccgaacaa ggcgcgaccc tgcccggtat gaccgtcgtc tgcggcgact cgcacacttc
    16021 cacccacggc gcattcggcg cactggcgca cggcatcggc acttccgaag tcgagcacac
    16081 catggcgacc caatgtatta ccgcgaaaaa atccaaatcc atgctgattg ccgttgacgg
    16141 caaattaaaa gcgggcgtta ccgccaaaga cgtggcgctc tacatcatcg ggcaaatcgg
    16201 cacggcaggc ggtacaggct acgccatcga gtttggcggc gaagccatcc gcagcctttc
    16261 tatggaaggc cgcatgactt tatgcaatat ggcgattgag gcaggcgcgc gctcaggcat
    16321 ggttgccgtc gaccaaacca ccatcgacta cgtaaaagat aaacccttcg cacccgaagg
    16381 cgaagcgtgg gacaaagccg tcgagtactg gcgtacgctg gtgtctgacg aaggtgcggt
    16441 attcgacaaa gaataccgtt tcaaagccga agacatcgaa ccgcaagtca cttggggcac
    16501 ttcgcctgaa atggttttag acatcagcag caaagtgccg aatcctgccg aagaaaccga
    16561 tccggtcaaa cgcagcggta tggaacgcgc ccttgaatac atgggcttgg aagccggtac
    16621 gccattaaac gaaatccccg tcgacatcgt attcatcggc tcttgcacca acagccgcat
    16681 cgaagacttg cgcgaagccg ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca
    16741 gcgcgtgtta atcgtccccg gctccggttt ggttaaagaa caagccgaaa aagaaggctt
    16801 ggacaaaatt ttcattgaag ccggttttga atggcgcgaa ccgggctgtt caatgtgtct
    16861 cgccatgaac gccgaccgcc tcgcaccggg gcaacgctgc gcctccacct ccaaccgcaa
    16921 ttttgaaggc cgtcaaggca acggcggacg tacccacctc gtcagccctg ccatggcagc
    16981 cgcagccgca gtgaccggtc gctttaccga catccgcatg atggcgtaag tattttcagg
    17041 cggtctttaa gaatttgacg cggccgtctg aaatatgtcc cgtaaggcag aaacaccttt
    17101 aatattcaaa ccaaggagtt taaccatgaa caaacttttc attaccgccc tgtccgccct
    17161 cgccttgtcc gcctgcgccg gcacttggga gggcgcgaaa caagacaccg cccgcaacct
    17221 tgacaaaaca caggccgccg ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa
    17281 aggctgggac aaaaccaaag aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat
    17341 ttcccatctc ggcggaaaaa tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg
    17401 aaaccgcttc agacggcatc gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa
    17461 ttaccgccat cgtcgccccg ctcgaccgca gcaacgtcga taccgacgcc atcatcccca
    17521 aacaatttct gaaatccatc aaacgcagcg gtttcggccc caatgccttt gacgaatggc
    17581 gttacctcga ccacggcgaa ccgggcatgg acaacagcaa acgcccgttg aaccctgatt
    17641 tctcgctgaa ccagccgcgt taccaaggcg cgcaaatcct gttgacgcgt aaaaacttcg
    17701 gttgcggctc ttcacgcgaa cacgcccctt gggcattgga cgactacggc ttccgcgccg
    17761 tcatcgcccc cagcttcgcc gacatcttct tcaacaactg ctacaaaaac ggccttttac
    17821 ctatcgtgtt gaccgaagaa caggttgacc ggcttttcaa agaagttgaa gccaacgaag
    17881 gctatcagct ctccatcgac cttgccgaac aaaccctgac cacccctggc ggcgaaacat
    17941 tcacattcga cattaccgaa caccgcaaac actgcctctt aaacggcttg gacgaaatcg
    18001 gactgaccct gcaacatgcc gatgaaattc acgcctttga agaaaaacgc cgccaaagtc
    18061 agccttggct gtttaacggt tgatatacaa aaggctgtct gaaaaattta agtgtttcag
    18121 acggccttta atctacaaaa atataccatg attaccgaca ccataagcaa tgccacccgc
    18181 tacgctgcct tgcatcccga ctttgccgag gccttccatc tgttacagac cttggatttt
    18241 gccaagctgc ccgacggtca ggtgccatgc aagaatccca atatccgtat ttttatcggt
    18301 agcgagccga tgaggacgaa gggagaagcc aagcctgaag cgcacttaaa acatattgat
    18361 attcaaacgc cgattgatgg cagcgaaaca tatggctgga tagacagagg ccgtctgaaa
    18421 aacggtttgg gctacaacga aaagcgcgat atcgagtttt tcaactgcga gccagaaact
    18481 tggctgacct tggaaccagg cgaatttgcg ctattttttc caaatgatgc gcatgcacca
    18541 ttagtcggtg acaaggcaaa catacgcaaa gcggtattta aaatccgtat cgaaacagag
    18601 cgtctataac ggcatcttgg cagcgtatcc gacctctaaa cctgcccgaa cttttttcag
    18661 acggcatccc cactctttat tgacaaaaaa aggaaaaaca aaatgaccaa acatatcgct
    18721 atcttgcgcg gcgacggcat cggcccagaa atcgtcgccg aaaccgtccg cgtactcgac
    18781 aaacttatcg cccaaggctt ggatgccggc tacgaatacg ccccattggg cggcgaagcc
    18841 tacgacgaat acggccatcc ctatcccgaa ttcacgcaaa acctctgccg caaagccgat
    18901 gccgtcctgc ttggtgcagt cggttctcct caatacgaca acctcgaccg tccgttgcgc
    18961 cctgagcgcg gcttgttggc tatccgtaag gacttgaatc tgtttgccaa cctgcgccct
    19021 gccatccttt atcccgagct ggccaacgcc tccacactca aacccgaaat cgttgccggt
    19081 ttggatattt tgattgtgcg cgaactcacc ggcgatattt atttcggcga accgcgcggt
    19141 atccgcgttt tggaaaacgg cgaacgcgaa ggctacaaca ccatgaaata cagcgaaagc
    19201 gaaatccgcc gcatcgccca cgttgccttc caatccgccc aaaaacgcag caaaaaagtc
    19261 tgctccgtcg gtaaagccaa cgttttggaa accaccgaac tgtggcgcga aatctttgaa
    19321 gaaatcggca aacaataccc cgatgtcgaa ctctcccaca tgtatgtaga caacgccgcc
    19381 atgcagctcg ttcgcgcgcc caaacaattc gacgtcatcg ccaccggcaa catcttcggc
    19441 gatattttat cggacgaagc ctctatgctg accggctcca tcggtatgct gccttccgct
    19501 tctttggacg aaaacggcaa aggcctgtac gaaccgtctc acggctccgc ccccgacatc
    19561 gccggacaaa acaaagccaa cccgctggcc accatcctct cgctcgccat gctgctgcgt
    19621 tacagcctga acgacgaagc ccgcgcgcaa caagttgaaa acgccgtaca aaaagtactg
    19681 caacaaggct tgcgtaccgg cgatatttac gaagaaggca cgaaactcgt ttcctgctcc
    19741 gaaatgggcg atgcggtact cgccgccttg taaaaaaaca aagcgcggat cacaccgcgc
    19801 tttttcatct gcccttttcc gctcgccccg ttcgtcaacc gcctgcaaac ccaccgttta
    19861 ccggcaggaa atgccataaa tccccgccta tgttaggata aacgccttta ctggctcaca
    19921 caaaggatta tcatcatgaa aaaaatcatc ttcgccgcac tcgcagcggc agccgttggc
    19981 actgcctccg ccgccaccta caaagtggac gaatatcacg ccaacgcccg tttcgccatc
    20041 gaccatttca acaccagcac caacgtcggc ggtttttacg gtctgaccgg ttccgtcgag
    20101 ttcgaccaag caaaacgcga cggtaaaatc gacatcacca tccccgttgc caacctgcaa
    20161 agcggttcgc aacactttac cgaccacctg aaatcagccg acatcttcga tgccgcccaa
    20221 tatccggaca tccgctttgt ttccaccaaa ttcaacttca acggcaaaaa actggtttcc
    20281 gttgacggca acctgaccat gcacggcaaa accgcccccg tcaaactcaa agccgaaaaa
    20341 ttcaactgct accaaagccc gatggcgaaa accgaagttt gcggcggcga cttcagcacc
    20401 accatcgacc gcaccaaatg gggcgtggac tacctcgtta acgttggtat gaccaaaagc
    20461 gtccgcatcg acatccaaat cgaggcagcc aaacaataaa tcttgatgcg aatcagcaca
    20521 aatgccgtct gaacgtcctt caaacggcat ttgtgccgtc ttcatcaaac cgccccgcca
    20581 acggtaagcc gatatggcgg agcacaatcc gccgaaagat atttcagcgg caggcagggc
    20641 ttgccggaaa gtccggaaag tatagtggat taacaaaaac cagtacggcg ttgcctcgcc
    20701 ttag
//
LOCUS       DAXHRI010000020        20688 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXHRI010000020 DAXHRI010000000
VERSION     DAXHRI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20688
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.020"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(64..240)
                     /locus_tag="ACFM75_000564"
     CDS             complement(64..240)
                     /locus_tag="ACFM75_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255155.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(243..1601)
                     /gene="murF"
                     /locus_tag="ACFM75_000565"
     CDS             complement(243..1601)
                     /gene="murF"
                     /locus_tag="ACFM75_000565"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC6255156.1"
                     /translation="MKPLDLNFICQALKLPMPSESKLVSRIVTDSRDSRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNVLRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGVDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1726..3372)
                     /locus_tag="ACFM75_000566"
     CDS             complement(1726..3372)
                     /locus_tag="ACFM75_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6255157.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3395..4873)
                     /locus_tag="ACFM75_000567"
     CDS             complement(3395..4873)
                     /locus_tag="ACFM75_000567"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC6255158.1"
                     /translation="MFSKLTPLAETGIPPLSCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAIANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGMLAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALKETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAIINVDDEYGAELVCRLKKDCPDLAVYSYGFSEQADIRIIDF
                     TASSDGIEAVFRTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4898..6643)
                     /locus_tag="ACFM75_000568"
     CDS             complement(4898..6643)
                     /locus_tag="ACFM75_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC6255159.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALVNTPAYEPNKPGQADSEQRRNRAVTDMIEPGSAIKPFVIAKALDADKTNLNERLNT
                     QPYKIGPAQVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKASTARQVRELMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNGR
                     YVDNKHVATFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVAGSPFKKIMGGSLNILGV
                     SPTKPLTAAAVKTPS"
     gene            complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="ACFM75_000569"
     CDS             complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="ACFM75_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC6255160.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="ACFM75_000570"
     CDS             complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="ACFM75_000570"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC6255161.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRRIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="ACFM75_000571"
     CDS             complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="ACFM75_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC6255162.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVSAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8681..9898
                     /locus_tag="ACFM75_000572"
     CDS             8681..9898
                     /locus_tag="ACFM75_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6255163.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            10030..10106
                     /locus_tag="ACFM75_000573"
     tRNA            10030..10106
                     /locus_tag="ACFM75_000573"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10064..10066,aa:Met,seq:cat)
     gene            10114..10189
                     /locus_tag="ACFM75_000574"
     tRNA            10114..10189
                     /locus_tag="ACFM75_000574"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10147..10149,aa:Ala,seq:ggc)
     gene            complement(10471..11292)
                     /locus_tag="ACFM75_000575"
     CDS             complement(10471..11292)
                     /locus_tag="ACFM75_000575"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC6255164.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11325..11969)
                     /locus_tag="ACFM75_000576"
     CDS             complement(11325..11969)
                     /locus_tag="ACFM75_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6255165.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKVHLEDRAVAGKWALSQKGFDGKKLMRVYDSPEAAAAASKMQK
                     LTEQYGIDNTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQPPAVQK"
     gene            complement(11972..12817)
                     /gene="ftsN"
                     /locus_tag="ACFM75_000577"
     CDS             complement(11972..12817)
                     /gene="ftsN"
                     /locus_tag="ACFM75_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6255166.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREESDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12923..14419)
                     /locus_tag="ACFM75_000578"
     CDS             complement(12923..14419)
                     /locus_tag="ACFM75_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC6255167.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPSENTKLPDLVDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14433..14753)
                     /locus_tag="ACFM75_000579"
     CDS             complement(14433..14753)
                     /locus_tag="ACFM75_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC6255168.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15237..16763
                     /gene="putP"
                     /locus_tag="ACFM75_000580"
     CDS             15237..16763
                     /gene="putP"
                     /locus_tag="ACFM75_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC6255169.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            complement(16853..17020)
                     /locus_tag="ACFM75_000581"
     CDS             complement(16853..17020)
                     /locus_tag="ACFM75_000581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255170.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17012..20617
                     /gene="putA"
                     /locus_tag="ACFM75_000582"
     CDS             17012..20617
                     /gene="putA"
                     /locus_tag="ACFM75_000582"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC6255171.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRTQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYAGEAENTLPKDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRNDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVCYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETVGISHVAALSP
                     LNSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4879 a         5963 c         5007 g         4839 t
ORIGIN      
        1 tcgggcctgc gcccgtttga ggttggcttg ccgcccggca aggtttcgga cggcttttgc
       61 agattaacgt gcagggactt tctcaatcac gcaaatcggg taactccccg gatttttgcc
      121 gccacccaag tctaaacata aatcttcata aagatacgcc tgcgtctgca ttccggcata
      181 aaacgcgccg accagcgcgg caatcaacaa taaggcttta aaaaaacgtc gacttttcat
      241 tttcacttat cctccaatgc ctcgaccact tcttccatct gcataaagcg cgaacctttc
      301 accaacacgg tggcgcgttc gggcaaatcg tggcgcaaca cttgaatcaa cgggtctttg
      361 gcggcgaacc acaaaccgtc cgcgccaaat ttttccgccg cttcgacgct gttgtcgccg
      421 acaaaataag ccgcttcgat gccttggtcg cgagcatacg cgccgacttc ggcgtgcatg
      481 gcggcggctt cgtcctcacc cagttcgccc atatcgccca tcacgaaaat acgcggcgca
      541 ggcatacgcg ccaacacgtc aatcgcggct ttcatactgt cggggttcgc gttgtaggta
      601 tcgtcaatca gggttgcgcc tttgatgccg gatttgacgt tcagacggcc tttgatgttg
      661 ctgaagcctt gcaaaccttc cgccacatcg ttcaaactca aacccgcagc caaagccagc
      721 gcggcggcag cggcggcgtt gtggacattg tggcggccgg gaacaggcag caccacggcg
      781 gcgcgctcat cgccgcacac caaatcaaat tcgcacgaca acggtttcag cacgatattt
      841 tccgcgtgga catcgccgct atcgacgccg aaagtgcgcg tattcaaatt aagcgttgcc
      901 gttttgaaga cagccatatt ggcatcttct tgaggaatca gcgccatgcc gtctgaacat
      961 aagccttgat aaatctcgct tttcgctttg gcaatatcgc ccactccgtc gaaaccgcag
     1021 ccgacatggg cacgcaggac gttgttgacc aatgcggcat cgggtttggc gatttgcgtt
     1081 aaaaccgcca gttcgccgaa atggttcatg cccatttcaa tcacggcata gcggtgtttt
     1141 tcgtttaact tcaacaaagt cagcggcaat ccgatatggt tgttgaagtt gcctgccgtc
     1201 gccaacacgg catcatcgcc gaaacggcgg cgcaataccg cagccagcat ttccttcacc
     1261 gtcgtcttgc cgcccgaacc ggtaatgccg aacacaaacg gattcacatt atcgcgccac
     1321 gccttcgcca acatttgcaa cgcggtaagc gtgtcatcga ctttcaacgc gccatccatt
     1381 gcaacgcaat cttcgcgcga aaccacaacc gccgccgcac cagcagccaa tacgtcttca
     1441 acaaaatcat gcgcgtcaaa ccgcccgccc gccaatgcga aaaacacatc gcccgcgcgg
     1501 ctgtcgcggc tgtcggttac gatgcgcgac acgagtttgc tttcagacgg catcggaagc
     1561 ttgagggctt ggcagatgaa atttagatcc agtggtttca tatttacttt cgttaatatt
     1621 cgggcggcgg acacatcggt agcggctgat ttttttatcg cctgttttgc tgtggtaaaa
     1681 cacagattat tttcccattc tcattcggca ttttttctgt acgtatcatt ttttagacgt
     1741 atttttagtc gatttgcctt ttcccgcata ccacggcgcg gggtcgtcgg gcagtccgtc
     1801 gataaaggca aggttattgc cttcgccctg cacatcggga acattccccc aaaaatcata
     1861 gccgtcatcg ggcaactcgt cggtttcgat acccgtccaa ctgccgaatc cgcgtaaaaa
     1921 attaaacgcg ctgcgcctca ctttaatcat ttcgcgccgc gtgtcatcgg acgaaatttt
     1981 aaccagcggc acgccgtagc tttgacgctt ccacgcacca tgtgtcaacg ttctctcgcc
     2041 gcctttaccg acatgcatca aaccgtggtc ggaaaagtaa accaaagacc agctttcttt
     2101 attttcattc aatatcttaa ctgtatcttc taaaaattta tcggtttgcg cgatggtgga
     2161 aacatagcag gatatttttt cagtttgata ctgaaaccgc cgcgcatcct tatccaaacg
     2221 tgtgcaaaaa tcactgtgcg aacccatcag gtgcatcaca atcagccgag gcttcgtgcc
     2281 tgcatttcct atcaaaaccc gtttgaacgc cggcaacaaa aggcggtcgc tcaaccccgc
     2341 gcttttgcca taatcacccc tttgggtaaa ccacggataa tcgctgcgta aggcataggt
     2401 ggaaatttcg ttggcaaaat gccccaacat tccttgatta gacagccacg ccgtccgaaa
     2461 acccgcctgc ttcgccaagc tgacgatgtt attgttcggt tctcccggca gccccaaagt
     2521 ctgcggcagc gaaagattcg tcgcgtgggc ggtcgattgg taaccgttta tcaacagccc
     2581 tttggtctga ctcaaaaaag gcgtatcggg caatgggaaa ccgtaaacat tcatataatc
     2641 cgaacgcgcg ctctcaccga taaccacaac ataattttta tacttgggcg caacatgacg
     2701 gatatgccat gttgacgctt tttttgctgc ttccaaaatg tgggcgcgct tggcggcata
     2761 ttgtgccggc gcggaagcca aatcgtaata caggtcgaaa atattcaaca acaggccggc
     2821 atcaggttct cgcaaatctt tatcgctggc gattttatcc atcaccgcgc aggacaaaat
     2881 caaagtcaat aatatagtca gccatatttt gctgcggcgt ttatagtttt ttacgtcagc
     2941 aaataccccc cccgatacac aatatttcca aactgtcaag ccaaaaatga aaaataatgc
     3001 ctgcacaaaa taaagcgacc cgggaagatt gccgacaaat tcacgcgcct cggcaggatt
     3061 gctttccaat atcgaaccga ctatctgata agacggcgca ccatacagcc agccgaccgg
     3121 caaatatagg gcggttgtgc cgacgtaaat cagtaaaaca actgaagaaa cgcgcgggaa
     3181 accgcgtgcc aataataata aaaatacaac cgaagccaaa gcacccacgg cagaataacg
     3241 gtagccgtat tcatattcca agtgataacc tgatgcgatg gccgcgccaa aacaaaaggc
     3301 taaaagcgcg caaatgccca atgtattttt taatgtctga tccatatttt ttattcgggc
     3361 ggattgtttc atacgatgcc gtccgaatat cccattattt acgagttaac aaagcctgtc
     3421 cgacgatttc aagatcggaa aaacggtgct tcacgccttg tacatcctga tagttttcat
     3481 gccctttgcc ggcaatcagg atgatgtcgt ttgcggcggc ttgttcaacc gcataacgga
     3541 tggcggcggc acggtcggct tcgacgcatt cgggcgcggg aacggcaggc aggatgtcgt
     3601 tgatgatgtc gtgcggattt tccaaacgcg gattgtcgct ggtgacgacg actttatccg
     3661 cgccctgtac ggctgccgcg cccatcagcg ggcgtttgcc gcgatcgcgg ttgccgccgc
     3721 aaccgaatac gcaccacaaa gccgcaccct gcggtttgat ttcctgcaaa gtggagagtg
     3781 ctttttccag tgcatcgggt gtgtgagcat aatcgacaac gaccaaaggt ttgccgctgt
     3841 tcataatgca atccatacgg cctgaggcgg gacggatttt tgccagtaca tccaatacct
     3901 tatccagcgg atagccgttg gcacacagca aggcgatgca ggcggcgagg ttttgcgcgt
     3961 tgaaccgtcc gagcaggcgc gtgcggcatt tcccttcgcc ccaaggggtt cggaatacgg
     4021 cttctatgcc gtctgaagag gcggtgaaat cgataatgcg gatgtcggct tgctcgctga
     4081 agccgtaact gtaaacggcc aaatcaggac agtctttttt cagacggcat acgagttccg
     4141 cgccgtattc gtcatccacg ttgataatgg cgtgcttcaa gccatgccag taaaacaggc
     4201 gcgacttgat ggcaccgtag gcttccatgg tgccgtggta gtcgaggtgg tctcgggtga
     4261 gattggtaaa gattgcgctg cggaatggca cgccgttgac acgcgattgg tcaaggccgt
     4321 ggctggagac ttccatcgcg gcggctgttg cgccttgttg acggaaacgg tagagcaggg
     4381 tttggacatc gacgggggcg ggggtggtgt gcgtggtttc tttcaatgca ccccaaaagc
     4441 cgttgccgac cgtgccgata atggcggttt tttctcccaa caaatcggcg gcttgcgcca
     4501 gccattgtgt gatggaggtt ttgccgttgg tgccggttac gccccagact ttgaggccgt
     4561 ctgaaacgtt gccgtaaact tgcgccgcca acatgccggc acggtgtttc aaatccttaa
     4621 tcccttgatt ggggactttc cattcgggat tccacgcgaa tttaccgtcg tcgtcccaaa
     4681 aaacaaaagc cgcgccgttg gcaatggctg cggggatata actgcggccg tcggtatatt
     4741 cgccctgaca ggcaacgaaa atatcgcctt gtttgatttg gcggctgtct gaatgcaaca
     4801 aacgccctgc cgcgtttgca cacgacaggg gcgggatgcc ggtttcagcc aaaggggtta
     4861 acttgctgaa cataaaacaa tctcgttgat acttggatta agacggtgtt ttgacggctg
     4921 cggcggtcag gggcttggtc ggggaaacgc ccaagatgtt caggctgcca cccataattt
     4981 ttttgaaggg cgaccctgcc actacgccgc cgtagtaacc gtttgcagtc ggttcgtcaa
     5041 tggttaccgc cacaatcaca cgcggatttt tagccggggc aaaaccgatg aaagtggcaa
     5101 cgtgtttgtt atcgacgtaa cgaccgttaa ccaacttacg cgccgtaccg gttttcgcac
     5161 cgacgtcgaa accatctact gccccggcag tacctgtacc acctggttcg gttacagaaa
     5221 ccatcaactc acgcacctga cgtgcggtcg atgctttaaa tatacgtttg ccttgcggcg
     5281 caaccgcctg tttttcaaag ctgaccggca gcaaaacgcc gtcgtgcgtc agtgcggtat
     5341 aggcgcgcgc caattgcagc aggctcaatt gcaggccgta accgaaagac atcgtcgcct
     5401 gttcgatagg ccgccacctg cgccaatttc tcaacaaacc tgcagtttcg cccggaaagc
     5461 ccgagtgcat acgcacaccg atacccaatt catgatagaa gtcatacatt tcttcggcac
     5521 cgaaacgcgc agacagtttg cttgtaccga cattggaaga tttctgcatg ataccgcgca
     5581 catccaaaga aggataaaca tgggtatcgc gcacctgagc cggaccaatt ttataaggct
     5641 gtgtattcaa cctctcgttc aaattggttt tatccgcatc cagtgctttc gcaatgacaa
     5701 agggctttat cgccgaaccg ggctcgatca tgtcggtaac ggcacggtta cgcctctgtt
     5761 cactgtctgc ctgaccgggt ttgttgggct cataggcagg cgtattgacc aatgcgagga
     5821 tttcccccgt gcgggcatcc aaaaccacca ccgttccggc ttttgcctga tggtattcga
     5881 ccgccttgtt caactcttca taggccaagg tctgaatcct ctgatcgagg gaaaggatga
     5941 tgtctttgcc gtttttcggg gctttattgc gcggggagtc caagctgtcc acaatattgc
     6001 cctgccggtc ccgcaaaacg acttccgcgc cgtcttcgcc atgcaggctg tcttcaagcg
     6061 aaagttccaa accttcctga cctttgccgt caatatcggt aaatccgatg acgtgtgcaa
     6121 acaggttgcc catcgggtaa tggcgtttta attctttttc aaatacaaag ttttccaaac
     6181 ccaaggcttt gacctcttcg gcaaccttgg gatcgagctg ccgcttaatc cagataaacg
     6241 acttgccttt ctgttcgagc ttgttcctca aaacatcaac cggcacatcg acaagctcgg
     6301 acaggcgttc caattgtgcg gcagacggca tttccttcat ctctttaggc acggcaaaca
     6361 gggactccgt cggcgcactc aacgccaaaa ccgcaccgtt ccggtccgaa accgtaccgc
     6421 gtgtagccgg caatgtttga gtccgcacaa tccggttgtc gccctgttct ttcaaaaagt
     6481 tatacgttac cgtctgcaga tacagtccgc gagcaatcag accggcaaac aagaccgcta
     6541 ttgccatcag gacgaagctg atccgtccgt tactggtcat cggctttttg acctgctctt
     6601 ctttgggcag catccgaggc ttatattcgc tcttaatcaa catttttact tctcgttatt
     6661 attatcctga cgcaggaatc cgattccggc acacaggctg cttctatctt tgatgcgcca
     6721 ccataaaggt attgcccgaa accggcggat ggaggttttg tttttctgcc gccgccctga
     6781 tcgcttcgtg gttcgccaaa cgcgcctgtt gcagcctcat ttgcgcataa tcctgctcca
     6841 aggcgatttc ctgttttttc gccttatcca aagctgtgaa attgagcctg tactggtttt
     6901 gctgcatcac aacggaaaaa gcggaaacgc acaccgcaag cagcagaagg aaattcaatt
     6961 tgttcattgc cattcagacg tttttctctg tgattgttcc ggtatcggac cggcagtccg
     7021 ctccgccaca cgcaaaaccg cacttctcgc cctcggattg gcggcaattt ccgcctcacc
     7081 cggctttaat gccctgccca cgattttcag gggcagctcg ggcaaatccg cttccctgac
     7141 cgccgcccag cgcggcaggg gcgcgtgttg cgaatatttt ttgacaaact gcttcacaat
     7201 gcgatcttcc aacgaatgga aagcaatgac cgccaaacgt ccgccctctt tcagacgaca
     7261 catgacctgc ggcaatactg cccctacttc ttcaagctcg cggttaataa agatgcggac
     7321 cgcctggaag gtgcgcgtcg caggatcctg cccccgctcg cgagtacgga cgttttgtgc
     7381 cacgatctgc gccagcttgc gggttgtatc gattggactt tccgcccgtt gcgcaacaat
     7441 ggcgcgcgca atccggcggc taaaccgctc ttcaccataa ttcttgatta cctcgtgcaa
     7501 atcctgttcc gacgcaaccg ctatccactc tgcggcagac ataccgcgcg tcgtatccat
     7561 acgcatatcc aaaggggcat cgaaacggaa gctgaaaccg cggctgccgt catcgatttg
     7621 cggggacgaa atccccaaat caaacagcgc accgtccacc ttgccgatac ccaaaccgtc
     7681 caatgccgtc tgaaacgaag caaaaccgcc atgcacgaca ccgacccgtt tgtccgaacg
     7741 cgccagctct tctgccacag caatcgcctg cgggtctttg tcgaaaacaa tcaaccgccc
     7801 cgcatcgccc aaacgcgaca aaatcagccg ggaatgccct cccctgccga acgtaccgtc
     7861 cacatagaca ccgtcttcgc gcacggcaag cgcatccacc gcctcattca gcaagaccgt
     7921 gatatgccgg taactttctg ctccactcac aattgcaaat ccgtctgact caactggaag
     7981 gcaagttcgt caggatcgtc atccaaagcc tgaaccatct cagcctccca ctgctcgcga
     8041 ccccaaagct ccaaacggtt ggcacgaccg accaaaacga cttcacggtc gaaatccacc
     8101 ctcttcctca gtccggcaga aaccagcacc ctgccggcgc tgtcccattc caaaatttcc
     8161 gcgttatgca gcaaaagatt ttgaaaccgc cgcaaaacag ggttatccgc cacttttaag
     8221 tttaaaagtt gcgccgcaac cttttcccac tccgcaacag ggtacatcaa cagcttgtgt
     8281 ttcgactcga gcgttaccac tacggcaggc gtatagaggc gcgacagaat gtcacggaat
     8341 ttggcaggaa cagccaaccg ccccttactg tcgatgctta attcgtgtgc gccgccgaac
     8401 atgacatgtc ccaagccgaa atcaaaatca caagggtaaa agagacactt tgccccacaa
     8461 ttccccacca atcgacacta taagaaattt taaacactcg gtcaaatcag ggcatgaaaa
     8521 ctcattaaca tatctgaaat ttttattcct tttaaaaaca ataagataaa aaatgacgac
     8581 aacggcacgg cgggtgcggt acagaataat cgaaccaata aacaactata tatgattaat
     8641 ttaataatat aaacacaata tatagtatta agataaagcc atgacagcac ccgtaccaac
     8701 gtgtaatatg tcgggaaatc caataaattt acacaagcta acacttatca tgcccctccc
     8761 cctcccctct cccgaagcac ggcaattctc gctcaaactg caaaccctca ttgccgaaaa
     8821 aatcggcaaa cacggcaact ggattccatt ttcacgtttt atggaattgg ttttatacgc
     8881 tccgcaatac ggctactaca ccggcggcag ccataaaatc ggcaataccg gggattttat
     8941 taccgcaccg accctcacct ctctgtttgc acagacactg gcacgccaac ttcaagaact
     9001 tctatcccaa acggcgggca atatctatga attcggcgcg ggaaccggac agcttgccgc
     9061 cgatttgttg ggcagcattt cggacggcat cagccgttac tatattattg aaatatcgcc
     9121 ggagctggca gcacgtcaga aaaacctgat tcaagcacgc gcaccggaag catctcaaaa
     9181 agttgtccac ttgaccgcac ttcccgaagc gtttgacggc atcatcatcg gcaacgaagt
     9241 actcgatgcc atgcctgtcg aaatcgtccg taaaaatgaa ggcggctcat tcgagcatgt
     9301 cggcgtttgc ctagataatg accgttttac ctattcggca cgaccgctgc acgacttgca
     9361 gctatctgcc ttggcttccc tctattttcc tcaaacagat tatccctata ccagcgaact
     9421 acatccgcaa caatatgcct ttatccgcac ccttgcctca aggcttgaac gcggaggtat
     9481 gatattcatc gactacggtt ttgatgcagc gcagtattac caccctcaac gcaatcaagg
     9541 tactctgatc ggacactacc gacatcacgt tatccacaat ccttttgact tcatcgggtt
     9601 ggccgacctg accgcgcatg tcaactttac cgacattgcc caagccggga cggatgccgg
     9661 attagatttg ataggttacc ttccccaatc ccatttctta ttgaacctgg gcattaccga
     9721 gctattggca cagacgggga aaacggattc ggcagcctat atccgtgaag ctgctgccgt
     9781 tcagaaactg attgaccagc atgaaatggg cgaactgttt aaagtcatcg cattcggaaa
     9841 aaatatcggc atcgactggg caggattccg cttcggcgac atctgccaca aactctaacc
     9901 ctcatgccgt cttaaccatt ttcagacggc ataaaatttt taatatttaa aaacaggaca
     9961 ctaatttaaa attagaaaat acggcttgtc aaaaaaacaa aaaaacatat aatagcgact
    10021 tcacgaaacg gcgaattagc tcagtcggtt agagcagagg aatcataatc cttgtgtccg
    10081 gggttcgagt ccctgattcg ccaccaaatt ttcgggggta tagctcagtt ggtagagcgc
    10141 ttgcatggca tgcaagaggt cagcggttcg attccgctta cctccaccag ataaaaaagc
    10201 acagaccgta aaaaggtatg tgctttttta ttgcctgatt gccagcaaat aaagaataaa
    10261 ccactgcctt caaaacaggc aatcgacttt aaacctatcg ccccgcctgt cctgatttta
    10321 tagtgaatta aatttaaacc ggtacagcgt tggctcgcct tgccgtacta tctgcggctt
    10381 cgtcgccttg tcctgatttt tgttaattca ctatatcagc ccgccagaca aacccgaccc
    10441 gaataatgtc ttcaggtcgg gtttatggtt tcattcccaa cttatccagc ctgacagcca
    10501 caatataatg atggcaatac caaaaacaat gcggtaatag gcaaaaggaa tataattttt
    10561 cttggaaaca aacctcagca acgcttttac cgctaccaag cctgaaacaa aggcagcaat
    10621 aaagcctatc agaatcaaac cgacatcatg cagggtgaaa aatcggtaat gtttcaggac
    10681 atcataagcc gttgcggcaa ccatcatcgg cacagccaag aaaaacgaga attctgtcgc
    10741 agttttccgt tcgatgcccc aaagcatccc gcccataatc gtactgcccg aacgggacgt
    10801 acccggaacc agtgcaaaca cttgggcaac gccgatcatc aaggcatcaa tcggacgcaa
    10861 tgcatcaaca tcggcaattt taggctctgc tcggctttgg cgtttctcca cccacaaaat
    10921 aaaaaaaccg cccaaaacca gcatgactgc aacactcaag gggttaaaca gatactcttt
    10981 gatttgtttg ccgaacaaca gccccatcac ggcggcaggt ataaaagcaa tggcaagatt
    11041 aaggacgaag cggttggctt tccggtcttt tcccaagccg tgcaacacat tgctgaaacg
    11101 ttgccggtat tcaaacacta ccgccaaaac cgcaccgagc tggatggcaa tttcaaaaac
    11161 cttgtgattg ctgtgaaaac caatcagatt gccgaacaca atcaaatgtc cggtgctgga
    11221 aatcggtaaa aattcggtaa aaccttctac caagcccatc atcagggctt tcaggacaat
    11281 cagaaaatcc attgcttgcg cttctttcgg atacgggagt tcggctattt ctgtacagca
    11341 gggggctgac gcttgcgttc ttccctgact ttggcaacca attctttaat cgtatgaacg
    11401 ccgccgtcaa agccattatt gaagataacg cggtattttc cgccgacaat aacggtcggc
    11461 gtgttgtcaa taccgtattg ttctgtcagt ttctgcattt ttgatgctgc tgctgcggcc
    11521 tcgggagagt cataaacgcg catcagtttt ttaccgtcaa agcctttttg agacaaagcc
    11581 cattttccgg ccaccgccct gtcttccaaa tgaaccttct gttcgtaaac tgctttaaac
    11641 acagcagggt ttgcctgata tttcaaaccc gacaaattga cggcggccgc catcctggcc
    11701 agaccgagca tttcaggctg ccagaccacg tgctccgtcc tcaaataggc atcagacggc
    11761 aatgccttgc ccaatttcaa taacaaagga tcgaaatgat ggcaatgtac gcagaaatag
    11821 ccgaaaaatt ccaaaacctc aattttaccc gactgttctt gaggaatggg tttatccaac
    11881 acaagatagt cttccccttc cgtcagggca tatgcctgcg cggacaacac tgccgacagc
    11941 agcagcggca acagatgttt gagcttcata attatttact ttcgatagaa cggatcaggc
    12001 tggcgacttc atgttttttc aactcgtcct gcattttttt caccgcatcg gcagacatat
    12061 tgccgctttg cacccggtaa agcgttttat gtcccgcctg atagccgacc accttggaag
    12121 atatgcccaa gattgccagt ttggcacgct gcccttccgc gctccggcgg tcggcatacg
    12181 cgcccatttg cagataatgc gttgcttccg ccttgtcgga cgttttcatt ttctgcactt
    12241 ctttggcggc ggcactgcgc gctttttcga tgctgccgct gttgaggatt tgttccgggg
    12301 tcggtttggg tgcaactttt tccttttccg cctttttctc ttcttttgga gcttttttct
    12361 ctgtttcttt agacggtttt accgcttgtt ttttaaccgt ttcggcatct ttcttctgcg
    12421 ctttttctct gacggtttgt tcacgctctt ccgtcagcac tttcttacgc actgcctgtc
    12481 cgtccgactc ttcccgttcc ggctcgcccg ccttttcttc aacctcgtcg gctttatcgg
    12541 cagctttttc cgcatccgac tgctctgcct ctgtcgcagc atccggttcg gacaaggcgt
    12601 tttgatcggc cggttcaggt tggatgtctt ccttaggctg gtttttcggt ttcaggattt
    12661 ccgtttctgc aggctgcttc gacgaagccg ggattttgaa cgcattttga ccgctctggt
    12721 tcagataaaa caaaataccg gcaataatga ccgtcgccag tatcaaaccg aagaaaaaac
    12781 cggacagacc ttttccggat tgggaaaatt tgttcataaa cataccttaa tgtgtttcag
    12841 acggcattaa cgccgtttgc ttacgggcgg atattctaac aatatcgcca tatttgggca
    12901 aaacctgctt ccattcccat tcctataaag cacgacggaa acctatggct ttcatgacgt
    12961 ggcttctgcc gacttcttcg tcgcccgcca aatccgccaa tgtacgcgcc acgcgcataa
    13021 tgcggtggaa gctgcgggcg gaaagggaga gtttttccag caggccgccc aatgcttcct
    13081 gcgcttcttt ttgaatgcgg gcggatgtgt cgagttcact gacactcaag gcggcattca
    13141 ctttgccttg ccgtgcgtat tgtttgtctc tagcggcgat aacgcgttcc aaaacggacg
    13201 cgctgctttc ccctgcttcc tgctgcatca gttcggcggc ggacaggctc gggacttcga
    13261 tggtcaaatc gatgcggtca agcaacgggc cggaaatttt gctgcggtaa cgcgcgacgc
    13321 tttcgggtgt gcagcggcag ggtttgacgg gatgcccgag atagccgcac gggcaggggt
    13381 tcatggcggc gacaagttgg aatttggcgg gatagacggc ttggcgcgcc gcgcgggaaa
    13441 tgtggatttc gccgttttcc aacggttcgc gcaaaacttc taaaactttg cggtcgaact
    13501 cgggcaattc atccaaaaat aaaacgccgt gatgcgccaa tgaaatttcg cccggacgcg
    13561 gatccgaacc gccgccgacc atagccgccg cgctggcgct gtgatgcgga ctgcggaaag
    13621 gacggttgct gtcgagttgt tgctggtggt tgggcaggag cgaacgcaat gcccaaactt
    13681 caaccaattc gtcttcggtc agcggcggca ggatgcctgg cagccgttgg gagagcatag
    13741 acttgcccgt tcctggcgga cccatcatca agaggctgtg tccgcctgcg gcagcgattt
    13801 ccaaagcaag gcgggcagtg tgctgaccct tgacatcgac gaggtcgggc aatttggtgt
    13861 tttcagacgg catctgagga acttggcatt cggtttgcgc caaaggttcg atgccgttca
    13921 aatgggcggc gacttcgccc aaagagcgcg cgccgtaaac ggtaatgccg cgcatcacgg
    13981 cggcttgtcc tgcattttct tcaggcaaaa caaatgcacg ttttgcctgc ataccctgcc
    14041 acgccatcgc caacgcgccg cgcacggggc gcaacagccc cgacagtgcc aattcccccg
    14101 caaactcgta ttccgccagt ttttcgggcg caacctgccc cgatgcagca aggatgccga
    14161 ttgcaatcgg caaatcgaaa cgccccgact ctttgggcag gtcggcgggg gcgaggttga
    14221 cggtaatttt tttggcgggg aattcaaaac cgctttgaat aatggcggca cggacgcggt
    14281 cgcgactttc tttgacttcc gtatcgggca gcccgacgat gttgaaatgc ggcaggccgt
    14341 tggcaaggtg ggcttccact tcgaccaacg gcgcattcat accgctcaag gcgcggctgt
    14401 aaaccaaggc aagcgacata tttcagacgg ccttattcgc cggcttcggt ttgctgcctg
    14461 atttcggcga cggcttcttc ggcagcggct tcagccgctt ccaatgctgc ccgttcggga
    14521 ttttgcgcgg cttcgagttt tgccaaacgc gcttccaaag ccgccagttt ggtacgggtt
    14581 ttgattaaaa cctgctgctg gatgtcgaat tcttcgcgcg taaccagatc catacggttg
    14641 aacgcgccgc ccagcatcgc cttaatattt ttttccacat ctttggcagg gctgttggcg
    14701 atggtttcgc tgattttcga accgacttct tcaaaaagct gcttgccgaa cataatctgt
    14761 atccttcctg aacatatcaa attcaatcgg ctattgtata aggaaaaatg ccgtctgaaa
    14821 acgggcggcg gataatcggc aaaacatacc gcgccccctt tgcggttgct aaattacacc
    14881 tcaaaacacc tgccgcctga aaacggattt catattccgc gcaacgccag attacaagac
    14941 actacaaaac aatatactgt tttaaatgat ttttccgacg cgcatcgttt caaactcggc
    15001 tttttaagcc attaagtgct ttgcaaacaa cagaaattgg gttatgctga aacggattat
    15061 ttacaatttc atatagtttt attacatatc ttattgtgat tgaagataat ttatccgaat
    15121 ccccctttcg ggtatccgga ttttccgttg tacttttatt tagaaaaaca tttcaggcgc
    15181 aagttgcttg caatttcaaa gcctgtaatt ctgaagtttt agacgaggaa tagcacatga
    15241 atcccatgta cattactttt gcaatctatc tggttgcagt ccttctcatc gggcttgccg
    15301 cctatttttc tacgcgcaat ttcgacgatt atattttggg cgggcgcagc ctgggcccgt
    15361 ttgttaccgc gatgtcggca ggcgcgtccg atatgtccgg ctggctgctg atgggtctgc
    15421 cgggcgcgat ttacctgagc ggtttgaatg aggcttggat tgccatcggc ctcttggtcg
    15481 gcgcgtattt caactggctc ttggtggcgg gccgcctgcg cgtgcatacc gaatacgcca
    15541 acaatgcgct gacgctgccg gattatttct tccaccgctt cggcgcgggc ggacacttga
    15601 tgaaagtggt ttccgcactg attatcctgt tcttcttcac gatttattgc gcctcgggca
    15661 ttgtggcggg cgcaaccctg ttccaaagcc tgtttgaagg tatgacttac aatcaggcaa
    15721 tgtggctggg cgcgggcgcg accatcgcct ataccttctt gggcggtttc ttggcggtca
    15781 gctggacgga tacgctgcag gcttctttga tgattttcgc cctgatttta acgccggtga
    15841 tggtctatct gggcttgggc ggcgcggaac agatgtctgc cgcgattcaa agcgtcgccg
    15901 caggcacggg caaagaatac ggcagcctgt ttgccggtac gaccgtcatc ggcatcattt
    15961 ccaccgccgc atggggcttg ggctatttcg gacagccgca cattttggcg cgctttatgg
    16021 cggccgaaag cgcgaaatcg ctggtatccg cacgccgcat cggcatgact tggatggcgt
    16081 tgtgccttgc gggcgcggta gcggtcggtt atttcggcat tgcgtatttc ggtgcaaatc
    16141 ccgacaaagt ttcttctatg agcggcaacc acgaacgcat cttcatcgcg ctttccaccc
    16201 tgctgttcaa cccttggatc gccggcatta ttttgagcgc gattttggcg gcggtgatgt
    16261 ccaccctgtc ctgccagctt ttggtttgct ccagcgcgat taccgaagac ttctacaaag
    16321 gctttttgcg taaaaacgcg caacagtcgg aactggtatg ggtcggccgc ctgatggtct
    16381 tggcgattgc cgtgatttcc atcctgattg cttccgatcc gaacagcaaa gtattgggac
    16441 tggtctctta tgcgtgggca ggtttcggtg cggcattcgg cccgattgtg attctgtccg
    16501 tattgtggaa acgcatcact gcctacggcg cgttgtcggg tatggttgca ggtgccttaa
    16561 ccgtggtcgt atgggcggaa tgggtgaaaa aaccggctca agcggcaggc gaaagcggct
    16621 tgttgacgat gtacgaaatc gtcccgggct ttattgtttg cttgatcgtt gccgtgttgg
    16681 tttccctgtt caacaaagaa ccttcgcgcg aaacccaaga acgctttgaa aaagccgacg
    16741 cagattaccg cgccgcccga taaacccctt tcagacggca cggcatcccg gttcgtgccg
    16801 tctgaacccg tttccgggtc aaacacaaat aaaccttgcc tgcatacagg ccttatttgt
    16861 gttttacccg tttcattttc aaagaaacgg aagcacctac cctgcccgca tttgaagcct
    16921 tgtatttcat ctgcggattt attacgatat taccgtattc aggccgcacc gatgccgcct
    16981 gccccccgaa aaactttgga gaatccaaaa aatgtttcat tttgcatttc cggcacaaac
    17041 tgccctgcgc caagcgataa ccgatgccta ccgccgtaat gaaatcgaag ccgtacagga
    17101 tatgttgcaa cgtgcacaga tgagcgacga agagcgcaac gccgcctccg agcttgcccg
    17161 ccgtttggtt acccaagtcc gcgccggccg caccaaagcc ggcggcgtgg atgcgctgat
    17221 gcacgagttt tcactctcca gcgaagaagg catcgcgctg atgtgtctgg cagaagccct
    17281 gctgcgcatt cccgacaacg ccacgcgcga ccgcctgatt gccgacaaga tttcagacgg
    17341 caactggaaa agccatttga acaacagccc ttccctcttc gtcaacgccg ccgcctgggg
    17401 cctgctgatt accggcaaac tgaccgccac aaacgacaaa caaatgagtt ccgcactcag
    17461 ccgcctgatc agcaaaggcg gcgcaccgct catccgccaa ggcgtaaatt acgccatgcg
    17521 gcttctgggc aaacagttcg taaccggaca gaccattgaa gaagccctgc aaaacggcaa
    17581 agaacgcgaa aaaatgggct accgcttctc cttcgatatg ttgggcgaag ccgcctacac
    17641 ccaagccgat gccgaccgct actaccgcga ctatgtcgaa gccatccacg ccatcggcaa
    17701 agatgcggca ggacaaggcg tttacgaagg taacggtatt tccgtcaaac tttccgccat
    17761 ccatccgcgc tactcgcgca cccaacacgg ccgcgtgatg ggcgaactgt tgccgcgcct
    17821 gaaagagctg ttccttttgg gtaaaaaata cgatatcggt atcaacatcg atgccgaaga
    17881 agccaaccgt ctggagctgt ctttggattt gatggaggct ttggtttcag accctgactt
    17941 ggctggctac aaaggtatcg gtttcgttgt ccaagcctac caaaaacgtt gtccgttcgt
    18001 tatcgactac ctgatcgacc ttgcccgccg caacaaccaa aaactaatga tccgcctcgt
    18061 caaaggcgcg tattgggaca gcgaaatcaa atgggcgcaa gtggacggct tgaacggcta
    18121 tccgacctac acccgcaaag tccacaccga catctcctac ctcgcctgcg cgcgcaaact
    18181 gctttccgcg caagacgcgg tattcccgca atttgccacc cacaacgcct acactttggg
    18241 cgcaatctac caaatgggta aaggcaaaga ttttgaacac caatgcctgc acggtatggg
    18301 cgaaaccctg tacgaccaag tcgtcggccc gcaaaactta ggccgccgcg tgcgcgtgta
    18361 cgccccggtc ggcacacacg aaaccctgct cgcctacttg gtgcgccgcc tgttggaaaa
    18421 cggcgcgaac tcgtctttcg tcaaccaaat cgtcgatgaa aacatcagca tcgacacgct
    18481 catccgcagc ccgttcgaca ccatcgccga acaaggcatc cacctgcaca acgccctgcc
    18541 gctgccgcgc gatttgtacg gcaaatgccg tctgaactcg caaggcgtgg acttgagcaa
    18601 cgaaaacgta ttgcagcagc ttcaagaaca gatgaacaaa gccgccgcgc aagacttcca
    18661 cgccgcatcc atcgtcaacg gcaaagcccg cgatgtcggc gaagcgcaac cgattaaaaa
    18721 tcctgccgac cacgacgaca tcgtcggcac agtcagtttt gccgatgccg cgcttgccca
    18781 agaagcggtt ggcgcagccg ttgccgcgtt ccccgaatgg agtgcgacac ctgccgccga
    18841 acgcgccgcc tgcctgcgcc gtttcgccga cctgctcgaa cagcacaccc ccgcgctgat
    18901 gatgcttgcc gtgcgcgaag caggcaaaac gctgaacaac gccattgccg aagtgcgcga
    18961 agccgtcgat ttctgccgct actacgcagg cgaagccgaa aacaccctgc ccaaagacgc
    19021 caaagcagtc ggcgccatcg tcgccatcag cccgtggaac ttcccgctcg ccatctttac
    19081 cggcgaagtc gtttccgcat tggcggcagg caacaccgtc atcgccaaac ccgccgaaca
    19141 aaccagcctg attgccggtt atgccgtttc cctcatgcac gaagccggca tcccgacttc
    19201 cgccctgcaa ctcgtcctcg gcgcaggcga cgtgggtgcg gcattgacca acgatgcccg
    19261 catcggcggc gtaattttca ccggctcgac cgaagtggcg cgcctaatca acaaagccct
    19321 tgccaaacgc aacgacaatc ccgtcctgat tgccgaaacc ggcggacaaa acgccatgat
    19381 tgtcgattcc accgcacttg ccgaacaagt ctgcgccgac gtattgaact ccgccttcga
    19441 cagcgcggga caacgctgct ccgccctgcg tatcttgtgc gtccaagaag acgttgccga
    19501 ccgtatgctc gacatgatca aaggcgcgat ggacgaactc gtcgtcggca aaccgattca
    19561 gctcactacc gatgtcggcc ccgtcatcga tgccgaagcg cagcaaaacc tgttgaacca
    19621 catcaacaaa atgaaaggcg ttgccaagtc ttaccacgaa gtcaaaaccg ctgccgatgt
    19681 cgattccgaa aaatccacgt tcgtccgccc catcctgttt gaattgaaca acctcaacga
    19741 actgcaacgc gaagtcttcg gccccgtcct gcacgtcgtc tgctaccgcg ccgacgaact
    19801 cgacagcgtc atcgaccaaa tcaacagcaa aggctacgcc ctgacccacg gcgtacacag
    19861 ccgcatcgaa ggcacggtac gccacatccg cagccgcatc gaagccggca acgtttacgt
    19921 caaccgcaac atcgtcggcg cagtcgtcgg cgtacagccc ttcggcggac acggtctgtc
    19981 cggcacaggt cccaaagcag gcggttcatt ctacctgcaa aaactgaccc gcgccggcga
    20041 atgggttgcc ccgaccctga gccaaatcgg acaggcggac gaagccgcac tcaaacgcct
    20101 cgaagcactg gttcacaaac tgccgttcaa cgccgaagag aaaaaagccg cagcggccgc
    20161 tttgggacac gcccgcatcc gcaccctgcg ccgtgccgaa accgtcctta ccggaccgac
    20221 cggcgagcgc aacagcatct catggcacgc gcccaaacgc gtttggatac acggcggcag
    20281 cacggttcaa gcctttgccg cactgaccga acttgccgcc tccggcatac aggcagtggt
    20341 cgaacccgac agccccttgg cttcctacac tgccgacttg gaaggtctgc tgctggtcaa
    20401 cggcaaaccc gaaaccgtcg gcatcagcca cgttgccgcc ctgtcgcctt tgaacagcgc
    20461 gcgtaaacag gaacttgccg cccacgacgg cgcactcatc cgcatcctcc cttcggaaaa
    20521 cggactcgac atcctgcaag tgtttgaaga aatctcttgc agcgtcaaca ccacagccgc
    20581 cggcggcaac gccagcctga tggcggtcgc cgactgattt taccgaaatc cccgggcgcg
    20641 gcccgtgaac caatgccgtc tgaaaacctt tcagacggca tttttata
//
LOCUS       DAXHRI010000021        20642 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXHRI010000021 DAXHRI010000000
VERSION     DAXHRI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20642
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.021"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(82..1128)
                     /gene="recA"
                     /locus_tag="ACFM75_000583"
     CDS             complement(82..1128)
                     /gene="recA"
                     /locus_tag="ACFM75_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC6255172.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1382..2146
                     /gene="aroD"
                     /locus_tag="ACFM75_000584"
     CDS             1382..2146
                     /gene="aroD"
                     /locus_tag="ACFM75_000584"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC6255173.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETSVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            2166..4181
                     /gene="rep"
                     /locus_tag="ACFM75_000585"
     CDS             2166..4181
                     /gene="rep"
                     /locus_tag="ACFM75_000585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC6255174.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGDPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINNLLKEIDYENHLLASEESKAGEIKWRNVGDL
                     VSWFARKGGEDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4249..5307)
                     /gene="dinB"
                     /locus_tag="ACFM75_000586"
     CDS             complement(4249..5307)
                     /gene="dinB"
                     /locus_tag="ACFM75_000586"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC6255175.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFELHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPYKVMAFLETLPLGKIPGVGKVTLKKMQSLGMQTAGDLRRFDRGELLNHFGR
                     YGYRLYDLARGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQGLWA"
     gene            5288..5572
                     /locus_tag="ACFM75_000587"
     CDS             5288..5572
                     /locus_tag="ACFM75_000587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255176.1"
                     /translation="MILREDIGSAEDKKDILLPASGKFQIYAPIWEAPHPQRWKQANA
                     CKQSRQTDNGILPDCHSDIGNAEKNTQIGYNPDKHTQGQQHGSLARSKIH"
     gene            5541..6320
                     /locus_tag="ACFM75_000588"
     CDS             5541..6320
                     /locus_tag="ACFM75_000588"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6255177.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVILCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPDMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(6530..7915)
                     /locus_tag="ACFM75_000589"
     CDS             complement(6530..7915)
                     /locus_tag="ACFM75_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC6255178.1"
                     /translation="MIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFPALGRLHAHK
                     VKAELVRHYGVDCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVTDNPPTGLFA
                     NKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCSVYVSGRCDKACDRSDEQVLAF
                     AHKLPVLDWGKMHEVEITETDPLTGKTVVLHGMGGALEMDDGLWYFDKDLPDVFKAKF
                     KTDRKNIKEIG"
     gene            8033..8109
                     /locus_tag="ACFM75_000590"
     tRNA            8033..8109
                     /locus_tag="ACFM75_000590"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8067..8069,aa:Arg,seq:acg)
     gene            8127..8201
                     /locus_tag="ACFM75_000591"
     tRNA            8127..8201
                     /locus_tag="ACFM75_000591"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8160..8162,aa:Glu,seq:ttc)
     gene            8760..9935
                     /locus_tag="ACFM75_000592"
     CDS             8760..9935
                     /locus_tag="ACFM75_000592"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC6255179.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(10276..10824)
                     /locus_tag="ACFM75_000593"
     CDS             complement(10276..10824)
                     /locus_tag="ACFM75_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC6255180.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(10827..12323)
                     /gene="ccoG"
                     /locus_tag="ACFM75_000594"
     CDS             complement(10827..12323)
                     /gene="ccoG"
                     /locus_tag="ACFM75_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC6255181.1"
                     /translation="MTTENQAGNPESGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            12852..14831
                     /gene="tkt"
                     /locus_tag="ACFM75_000595"
     CDS             12852..14831
                     /gene="tkt"
                     /locus_tag="ACFM75_000595"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC6255182.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEKGAKLEAEWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKHGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            14979..16367
                     /gene="fumC"
                     /locus_tag="ACFM75_000596"
     CDS             14979..16367
                     /gene="fumC"
                     /locus_tag="ACFM75_000596"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC6255183.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(16432..17355)
                     /locus_tag="ACFM75_000597"
     CDS             complement(16432..17355)
                     /locus_tag="ACFM75_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6255184.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALIVVGGAVTAAVGDRLFKRR"
     gene            17527..17667
                     /locus_tag="ACFM75_000598"
     CDS             17527..17667
                     /locus_tag="ACFM75_000598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255185.1"
                     /translation="MPSENSGDVRQASLPQNARTAQSQRSAQRKGRQQAGSDAKKRNP
                     AP"
     gene            complement(17727..18251)
                     /locus_tag="ACFM75_000599"
     CDS             complement(17727..18251)
                     /locus_tag="ACFM75_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC6255186.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(18254..19636)
                     /locus_tag="ACFM75_000600"
     CDS             complement(18254..19636)
                     /locus_tag="ACFM75_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6255187.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(19747..20370)
                     /locus_tag="ACFM75_000601"
     CDS             complement(19747..20370)
                     /locus_tag="ACFM75_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC6255188.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
BASE COUNT         5081 a         5958 c         5118 g         4485 t
ORIGIN      
        1 aaaactcaaa tcaagccgtt cggaggcggc tcaaaaaacg gtacttcgca gcagaagtac
       61 cgtttatcgg gatttcaggt tttattcttc ggggcgttcg ccgtcggttt cgtcctgcgt
      121 cccttcggtg atgtgcattt ctacgccgtt gagggcgcgg atttttgcgt cgatttcatc
      181 agcaatttcg ggattttcct tcagccagac gcggacgttg tctttgccct gaccgatttt
      241 cgcgccgttg tagctgtacc acgcgccgga tttgttgatg atgtcgtttt tcacgccgat
      301 gtcgatcaat tcgccttccc aactgatgcc ttctccgtag aggatgtcaa actctgcctg
      361 acggaacggg ggggcgactt tgtttttgat gactttgacg cgggtttcgt tgcccaatac
      421 ctcttcgcct tttttgatgg atccggtgcg gcggatgtcg aggcggacgg aagaatagaa
      481 tttcagcgcg ttgccgccgg tggtggtttc ggggctgccg aacattacgc cgatcttcat
      541 ccggatttgg ttgatgaaca caaccagcgt gttggttttt ttgatgtgtc cggtcagttt
      601 gcgcaaagcc tggctcatca ggcgcgcctg cagtccgaca tggctgtccc ccatatcgcc
      661 ttcgatttcg gctttgggga cgagtgcggc tacggaatcg acgactacca tatctatgcc
      721 gcccgaacgg acgagtgtgt cgcagatttc caaagcctgt tcgccggtat cgggctggga
      781 caggtaaagc tcttcgactt ttacgccgag tttgcgggcg taaacgggat cgaaggcgtg
      841 ttcggcatcg acaaaggcgc acacgccgcc gtttttctgg cattgggcaa cggcttcgag
      901 gcagagggtg gttttgccgg aggattcggg gccgaagatt tcgacgatgc gcccgcgcgg
      961 cagaccgccg actccgagtg cgaggtctaa tccgagcgat ccggtggaaa tgacttcgag
     1021 gttttcttcc tgctggctgc cgtccatttt catgatggcg cctttgccga aacttttttc
     1081 gatttgcgcc agtgcggcgg caagggcttt gcttttgtcg tctgacattg gggttactcc
     1141 ggaacaaatg cggtatgtgg gatgcggcgc aacacgggct gcggcgcggg atgtgtatcg
     1201 ttttcccgat gtgcgggcta tcggtaatgc tgcttcacga ggttgccatt atcgcatatt
     1261 tccttgcttg ccgatatgcg gcaggacgcg gcggcttgtg ccggaatgga atctggatgc
     1321 cgtctaaaag gcggccggct ttgttataat ggcggctgtt ttttctgtgt gtgcctgttt
     1381 tatgtgttcc tgccttgttg tcaaaaatac cgttatcgga agcggacgca ccaaaatcgc
     1441 cgtgccgctt gtcgcccgcg atgccgccga actttccgcc gtacttgacc aaatcaaaaa
     1501 cctgcccttc gacatcgtcg agttccgcgc cgattttttg gaatgcgcgg gcagcatcgg
     1561 cgaagtattg cgccacacgc agaccgtccg cgacgcgctg cccgacaaac cgctgctgtt
     1621 tacgttcaga cggcatggcg aaggcggctc gttcccgtgt tcggacgatt attatttcaa
     1681 actgctcgac gcgctgatcg aaagccgcct gcccgacatt atcgatatcg agctgttttc
     1741 cggcgaaacc tccgtccggc gcgccgtggc aaatgctcaa aaaaacggca tcgccgccct
     1801 gctctgcaat catgagtttc accgcacgcc gccgcaagaa gaaatcgtat gccgtctgaa
     1861 acagatggag gactgcggcg cggacatctg caaaatcgcg gtgatgccgc aaagcgcgga
     1921 agatgtgctg actttgcttt ccgccacgct cgaagcgaaa cggcttgccg ccaaaccggt
     1981 tgttacgatg tcgatggggc agacgggtgc ggtcagccgc cttgccggac aggtgttcgg
     2041 ctcaagcatc acgttcggtt cgggaacgca aaactccgcg ccggggcaaa tcggcgtatc
     2101 cgccctccgt gcggcactcg actgtctcga aagcggcgca gactgatttc agacagcatc
     2161 aaaacatgat gaaactcaat ccccaacagc tcgaagccgt ccgctacctc ggcggcccac
     2221 tgctcgtcct tgccggtgca ggcagcggca aaaccggcgt gattactcaa aaaattaagc
     2281 atttgattgt caatgtcggc tacctgccgc ataccgttgc cgcaattacc tttaccaaca
     2341 aagccgctgc ggaaatgcag gagcgcgttg ccaaaatgct gcccaaaccg caaacgcgcg
     2401 ggctgacgat ttgcacgttc cactctttgg gcatgaagat tctgcgcgaa gaggcgaacc
     2461 atatcggtta caaaaaaaac ttctccattc tcgattctac cgacagcgcg aaaatcatcg
     2521 gcgaactctt aggcggtacg ggcaaagaag ccgtattcaa ggcgcaacat caaatctccc
     2581 tgtggaaaaa cgatctgaaa acgcctgaag atgtcgttca gacggcctct aatgcgtggg
     2641 aacaacaaac cgcacgcgtg tatgcgagct atcaggaaac cttacaaagc tatcaggcag
     2701 tggacttcga cgacttaatc cgcctgcctg ccgtgctgtt gcagcaaaac agcgaggtgc
     2761 gcaacaaatg gcagcggcgg ctgcgttatc tgctggttga cgaatgccaa gatacgaata
     2821 cctgccaatt tacgttgatg aaacttctga ccggcgcgga aggtatgttt accgccgtcg
     2881 gcgacgacga ccagtccatc tacgcatggc gcggcgcaaa catggaaaat ctgcgcaaaa
     2941 tgcaggaaaa ctatccacag atgaaggtca tcaaactgga gcaaaactac cgctccaccg
     3001 cgcggattct caaaatcgcc aacaaagtca tcgaaaacaa ccccaagctg tttaccaaaa
     3061 aactttggtc gcaattgggc gaaggcgatc ccgtcaaagt cgttgcctgc caaaacgagc
     3121 agcacgaagc cgactgggtc gtcagccaaa tcgtcaaaca aaaactcatc ggcggcgaca
     3181 aaacccaata cgccgatttc gccgtgttat accgcggaaa gcatcaggcg aggattttcg
     3241 aggaagcatt gcgcggcgcg cgcatcccct accgtctctc cggcggacaa agctttttcg
     3301 ataaagccga aatcaaagac gtgttgtctt atgtgcggct gcttgccaac cccaacgacg
     3361 atcccgcctt tctgcgtgcc gttaccacgc ccaaacgcgg catcggcgat gtcacgctgg
     3421 gcaagctcaa cacttacgcg cacgaacacg aatgcagcct gtatgaagcc gcacaaaacg
     3481 aagaagccct tgccacgctg aacaatacca accgccaaca cctgcaagcc tttatggata
     3541 tgttcggcag ctaccgcgcc aaagcggaaa ccggcgaagc aggcgagttc atcaacaacc
     3601 tgcttaaaga aatcgactac gaaaaccatc tgcttgccag tgaagaaagc aaagcaggcg
     3661 aaatcaaatg gcgcaacgtc ggcgatttgg tatcatggtt tgcgcgaaaa ggcggggaag
     3721 acggcaaaaa cgtcatcgaa ctcgcccaaa ccgtcgcctt gatgacgctt ttggaaggaa
     3781 aagacgaaga agaaaccgat gctgtctcgc tatccacgct acacgccgcc aaaggtttgg
     3841 agtatccgta tgttttcctt gtcggttgcg aagaaggtgt tttgccgcac aacgacagca
     3901 tcgaagaggg caacgtcgaa gaagaacgcc gcctgatgta cgtcggcatc acccgcgcca
     3961 aacgccagct tacgctgacc cactgcgtca aacgcaaaaa acaaggcata tggcagttcc
     4021 ccgaacccag ccgattcata gacgaaatgc cgcaggaaga tttgaaaatc ctggggcgca
     4081 aaggcggcga accgattgtc agcaaggaag aaggcaggcg caaccttgcc gatataatcg
     4141 gaaggctcga caacctaaaa aaaagcggcg cggcggatta aaccggagcc gcaatgccgt
     4201 ctgaaggctt cagacggcat attttttgga cggcgcgcgt aaatcggttt acgcccacaa
     4261 gccctgctgc tggtttttcg gcacaagatg tcccacgccg atgccgataa ggcggaacgc
     4321 gtcttccgtc tgcggcggga cacgcgccat caacatttgc gcagcctgca gcagagctgc
     4381 gcagtcgggc aatacggagg aataagtcag cgtgcgcgtg atgatgcgga aatcgtaggt
     4441 cttcagcttg agcgttacgc tttgggcttc gacgtttttg cgcgtgattt gccgccacag
     4501 gtcttcggca agatggggca ggtgtccggc agcctgctcg agcggcaggt cttcaggcag
     4561 ggtgatttct gtggagattt ggaggcgttc gcgttcggct ttgacggggc gttcatccgt
     4621 accgcgcgcc aaatcataga ggcggtagcc gtagcgtcca aaatggttta agagctcgcc
     4681 gcgatcgaaa cggcgcaagt cgcccgccgt ctgcataccc agcgactgca tttttttcag
     4741 cgttaccttg cccacgccgg ggattttgcc caaaggcagg gtttccaaaa atgccatgac
     4801 tttgtacggc ggcaacacaa actgcccgtt cggcttgcgc cagtccgacg cgattttcgc
     4861 cagaaatttg ttcggcgcga tgcctgcgga tgcagtcaaa cctgtttccg caaaaatggc
     4921 ggcacggatt tctttggcaa cttcactggc gtaagggatg tttttgaaat tacgggtaac
     4981 gtcaagatag gcttcgtcca gcgacaaggg ttcgattaaa tcggtataac gcctgaatac
     5041 ggcgtgaatc tgcgcggaaa cctgacggta caaatcgaaa tgcggcggca catacaccgc
     5101 ttgcggacac agccttttcg ccgttgccac cgacatcgcg gaatgcagct cgaactgccg
     5161 tgcctcatac gatgcggcgc aaatcaccga acgcgcgccc tcccacgcga cgaccaccgg
     5221 ccgccctttc aaatgcggct gttcgcgcag ctctaccgat gcgtagaatg cgtccatgtc
     5281 gatgtggatg attttgcgtg aagacatcgg ctctgctgag gataaaaagg atattctact
     5341 gccggcatcg ggcaaattcc aaatatacgc cccgatatgg gaagccccgc atccgcagcg
     5401 ttggaaacag gcaaacgcct gcaaacaaag ccggcaaacg gataacggca tattgcctga
     5461 ttgtcattca gacataggaa atgcggaaaa aaacacccaa attggctata atcccgacaa
     5521 acacactcaa ggacaacaac atggcagcct cgcccgaagc aaaattcact gaagaaaaga
     5581 ttttgtgggt caaacaccac acgccgaaac tcatcacttt cgccatcagc cgtcccgaat
     5641 cctaccgttt taaagccgga cagttctccc gactcggttt ctacgaaggg gaaggcttta
     5701 tttggcgtgc ctactccatc gtttccgcag aatatgcaga cacgctcgaa tattttgccg
     5761 tactcatcca agacggcccc atgtcggccc gtttcgccaa aatgcaacag ggcgacacca
     5821 tcctgctcga taaaaatgcc accggcttcc tcctgcccga acgcttcccc gacggcaagg
     5881 atttggtgat actctgcacc ggctccggca tcgccccctt cctttccatt ctcgaacaac
     5941 ctgaaatccg acagcgtttc gataccgtca acctgataca ttccgtatct tttcccgaag
     6001 aattgatttt caacgaccgg ctcgccgcat tgtccgaaca tcccctggta ggcgaataca
     6061 gacactcgtt ccatttcgtc cctgttacca cccgtgccgc caacccctcg ggcttaagcg
     6121 gaaaacgcat tccggaactc ttaaaaaaca acagcatcga acaggcgctg cataccaagt
     6181 tcaccccgga atccacacgg tttatgattt gcggtaaccc ggacatggtc aaagacactt
     6241 tccaaacgct actcgacatg ggttacgcca tgcaccgcaa ccgcattccc ggtcaaatca
     6301 tgatggaaaa cggcttctaa aaaccaccct gcttgtccga tgccttcgga tggacgggca
     6361 aaccgacacg gcacgaaaac cgcgtcggca aaaatgccgt ctgaaaagtt cagacggcat
     6421 cttcggacac attacctgca aacggtaaca cattccaacc caaaccgatt aggcaatcag
     6481 tacgaacgac tgtttacata cttgccggct ttcaccaacc ggtattgatt taaccgattt
     6541 ccttaatatt tttcctgtcc gttttaaact tcgccttaaa cacatccggt aaatctttat
     6601 cgaaatacca aagcccgtca tccatttcca atgcgccgcc cattccgtgc agaacgactg
     6661 ttttccccgt cagcggatcg gtttcggtaa tttccacttc gtgcattttg ccccaatcca
     6721 aaacaggcaa cttgtgtgca aacgccaaaa cctgttcgtc agagcggtcg cacgccttat
     6781 cgcatcggcc tgaaacatat acggaacagg gctcatcctc tgcataacca tccggaagga
     6841 taccggctga ggcaggacac aatccgtatg tttccgccca cgacgacaaa gcccgtctcg
     6901 ccgccttttt attagcaaat aatccggtag gcggattatc cgtcacaccg tttttcaaag
     6961 ccgctgtttt cgcattcaac atgccgtctg aacctttttc aaacctgacg gtcgtaaatg
     7021 ttttaagcag atttttggca gatacgtaac aatccacccc ataatgcctg accaattccg
     7081 ctttaacctt atgggcatgc agcctgccca atgcgggaaa aaaacggact tcctccgtat
     7141 tgcaccaatc aaacggggct tttccggagt ccaataaagc cgaaacctcg ctatataccc
     7201 gttcaaacgt accgatgtaa tttactttcc ctccgccgtc gaaacaggcc agcaccccca
     7261 taccgtcagg caaaccgtac aactgttccc tcaaccgttc gggcagcgcg gcaggcaacg
     7321 gtttcggatt catcaaacgg aaacactgcc tgatccactc ctcgaccccg tgttccgaca
     7381 ggctgtattc caaataatca cacaacgccg acacatccgc catcgcacga tgcctgtctt
     7441 ccacaacaat ccccaacctt tcgatgatac tgtccaggct gtgcttgtaa aattgcggat
     7501 acagacaccg ggacagctgc acactgcaca aagcaggcga tgaaaatccg atacccgcac
     7561 gatgaaactc atgctttaaa aacgtatagt cgaaacggct gttatgtgca accagcacac
     7621 aacccttcaa taccgaaaac aactcgccgg caatctctgc aaaaacaggc gcatcggcaa
     7681 ccatgccgtc tgaaatcccc gtcagccccg ccacaaactg cggaatcggt ttttgaggat
     7741 taaccaacca ctcatgcctc accacccttc cctgctcaaa cttgaccaaa gccacttcgg
     7801 ttaccctgtc ttcatacaga ttgccgcccg tcgattccaa atcgaccacg gcaacaggca
     7861 ttccaaaccg taaaaatacc ttttccagca agggccagcg ggaagcaaca atcattttat
     7921 tctctttaaa ttcaaacaac aaaccgatat tttacacttt taaggcattt catccaacaa
     7981 aacaattgac agaatccgat gattacccta aaattcaaat ctttcttgca gtgcacccgt
     8041 agctcagttg gatagagtat ctggctacga accagagggt cgggcgttcg aatcgctccg
     8101 ggtgcgccag taagaaaata caatatgcgc ccatcgtcta gcggttagga catcgccctt
     8161 tcacggcggt aaccggggtt cgattccccg tgggcgtgcc aaattccaaa tcccccgaaa
     8221 taatccctcg gggattttta ttgcctcggc aattcattac catatcttta cctaccccct
     8281 tcatcagact ctcagacgta atcgaatcat attcaaacct ttgccgtgca aaccgatatc
     8341 acgtaaccgg atgcggtgtc cgtccaacat tttacccgat tgaaacgcct gatatattgc
     8401 accccatcaa cgtggcatta cttttcttaa caatcccctt tgacagcaac tgactagggc
     8461 ttttttatgc catcatcaaa tttatagtgg attaacttta aaccagtacg gcgttgcctc
     8521 gcctttccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
     8581 tcactataat attttctctc ccgattgaaa caggcgtaac agaatatgcc cgaagctccg
     8641 gctgctttct tgtttaccgc cgcgatattt agagtataat accaaatttg agcaatagtt
     8701 ctaaaacagt tagaaccatt tttcatgagc ctgactgatt cgtatactcg gagaaactga
     8761 tgcaaaatat ttttgaccct ttggttattc gtggaaaatc ccttaccccc atcgtgcaag
     8821 gcggtatggg ggtcggtgtt tccgcatcgg gtttatccag cgcggtggca cgtgaaaacg
     8881 gtatcggaac gattgccagt gtggatttgc gccaccttca cgaagaccta ctcgccgaat
     8941 cacaaatcaa tccgagtgaa gagaaatata catctttgaa ctgtaccgca ttagacaggg
     9001 aaatccaaaa agccaaaagc gcttcagagg gaaaaggact gattgcggtc aacgtgatga
     9061 aggcggtcaa agaccacgcc gcgtatgtcc gccaggcttg cgaatcaggg gcggatgcgg
     9121 ttgtaatggg tgccggcctg cctttagacc tgccggaaat gaccgagggc tatcataaag
     9181 atgtcgcgct gctgccgatt ctgtccgaat cgcgcggtat taatatcgtc ttgaaacgtt
     9241 ggatgaaaaa aggcatattg cccgatgcga ttgtagtcga acatcctgcc cacgcggccg
     9301 ggcatttggg cgcgtcaacc gtcgaaggcg taaacgatgc caagttcgac ttcaaacgcg
     9361 tgattgagga aacgtttgaa gttttcaaaa gtttagggct ggaaagcgaa aaaatcccgc
     9421 ttattcttgc gggaggcatg gcaaattttg aaaaagtcaa aaccgcccta aagaactggg
     9481 gagcatccgc cgttcaaatc ggtacggctt ttgccgttac cgaagaagga gatgcacacc
     9541 ttaacttcaa aaaaacgctc gccggtgcgg aaactgaaaa agtagtcgaa tttatgtctg
     9601 ttgccggttt gccggcgcgc ggtgtccgca ccaaattcct agacagctac atcaagcgtg
     9661 aaagcaaact tcagacaaac gccaaagccg acccgcgccg ctgtacccaa ggtttaaact
     9721 gcctaaccag ttgcggtctg cgcgacgggc tttccaaagc cggacaattc tgcattgata
     9781 tccagcttgc cgccgcattc cgtggagaag tagataaagg cctgttcttc agaggtaaag
     9841 acccgctgcc cttcggcaat gccatccgca ccgtccgcga gacgatacaa tatctgctga
     9901 cggggagcga acctgttgca acgctcggac gctgacatca ggtaggattt gatttgcaaa
     9961 caaaatgccg tctgaagcct ttcagacggc atttttcagg ctgcgtttcg gaatagtgtc
    10021 gaaaaaacgg agagaaataa aaacgttttt atgtaaacaa aacgccttcc gcattgtgtc
    10081 gggcatacaa cacaaatgcc gtctgaagcc cttcagacgg catttttcag gctgcatttc
    10141 ggaatggtgt taaaaaataa acgggataag atacatttat tttcgtccga taaatcagac
    10201 catcacgccg aataatcaaa caatgcctgc ccgacattgc atctggcaaa ccaatgcaat
    10261 gaaaacacgg cttttttatt tgcttccggc attattgaaa agtttatcca tcggggtcag
    10321 atccaccgca ttgccttggc tggtaatcca tttgttctcg acgcgccaca cgcctgccgc
    10381 gtcctccacg cgcacatacc agtggttggt cggcgaaagg gttttgaaca ccgcctcata
    10441 ttccgccctg ccgttctgcg cgctgccgac gggcttgagg gcgacggttt gatcgtccgc
    10501 cttgcgggtc gggtgcatca gcagcaggtt caaaggctgt ttgccgtcaa actcgccgcc
    10561 gacaaacact tttgccgcat tcatatcggg ggaaatgaga acctgcaccc cgatatgccg
    10621 tctgacggct tcttcatccc gatgaagctg gatgtcgata tgtttgccgt ctttataata
    10681 atcgtccgta accaaatctg tcgcgtgctg ctgcgcgaca aaaaacatag cgacgctggc
    10741 gatgacgaca aaaatcggcc ccgccatcaa gatccacggc cagacgtgtt tgtaccaagg
    10801 tttgattgga gtgttttgag acacggttat tctccgataa aggttgcatc ttcttccaag
    10861 acgaccggct tgccgtcggg cgcgccgctt tcgcggtatt ggaaggtaaa ttcgataggg
    10921 tggctgcctt tgtccgcgta ttccggaatg gtggacactt ggacgggaag ggttaccgtt
    10981 tcgcgcgggg caaccttgat accgccttcg ggcagcccgg tcagggcgat ttcgtcaaag
    11041 cctttgacac ttgcggtaat gagctgttct ttttcacttt tgttgatgat acgcaggctg
    11101 tatgcgtttt ccagccagcc tttggcgttt tcgcgcacca gtacgccacg gtctttcaaa
    11161 atatcgacct cgaccatttt gcgcgtggac aaaccggcca ggaaggcaat gataactaac
    11221 gccaacaccg cgccgtaacc tgccacacgc ggtctgagca gccgtttttt aatgtctttt
    11281 tcagaatatt cgtgttccag cgcgctttcg gtcgtataac ggattaatcc gcgcggatag
    11341 cccattttgt ccataatctc atcacacgcg tcgatacagg cggcgcagcc gatacattgg
    11401 tactgcagac cgttgcggat gtcgatgccg acagggcaga cttggacgca catcgcacag
    11461 ttgatgcagt cgcccaaacc cgcctcttcc ttgttgaccg ttttcttgcg ggctccgcgc
    11521 ggttcgccgc gttccgcgtc ataagaaaca atcagcgtgt ccttgtcgaa catcgcgctt
    11581 tggaaacgtg catacggaca catatgcagg catacttttt cgcgcataat atgtgcgaag
    11641 aagaaggtca taaagccgta aaacgctgtg gcaaacatcg cgccgccgcc tgctgctcca
    11701 gtgaataaat cgggaacgaa ctggcggata gggacaaacc agcctgcaaa cgtgatgccc
    11761 gtccacgcgc agacaaggaa aatcaacagg tatttggtgg ctttgatgcg gattttagtg
    11821 aaattccacg gcgatttttc cagcttcagg cgtttgtttc tatcgccttc gaccaggttg
    11881 tcgatccaca gcataatttc ggtgtaaacc gtttgcgggc aggaatagcc gcaccacagt
    11941 cgcccggcaa tcgtcgtcca ccaaaacagc ccgaaggcgc aaatcatcag cagcaaggca
    12001 aggtaaatca aatcgcccac ccccaacgac aatccgaaaa tgaagaaatg ccgttcgggg
    12061 atattgaaaa cgacggcctg cctgccgctc cagttgaacc acggaatgac gtaaaacaca
    12121 aactgcgtcg ccaatacggc ggcgatacgc agtttggcga accgtccttc cgcctttttg
    12181 ggatggatgc gttcgccttc gggatggatt tgaatcacgc tggctcgcgg atcgaatgtt
    12241 tttttcgctt tcggcgcggc ttttgtttgt tcggacgtgc cgattccgga ttccggattg
    12301 ccggcttggt tttccgtggt cattctgcat tccttagatt tttgattgat ggtttgcccg
    12361 ttaccgccgc cgtttgcttt tcagacgtca tttttcttgt tttttaaggc gttgtgtttc
    12421 aagttttgag aaaatccgtt tttcccaaaa tatatttccg ctattgtaca actttatgcg
    12481 ccgtccggat gtatggggcg gatacatttc ccatccgcat caaaacgcct ggattttacc
    12541 ttaccgcccg aacaaaatcc gaatacggtt aaaaaaaaaa gactaaaaaa accgacaccc
    12601 ccatatcggc agaaccgacg gtgcaagctc ataaacaaac gctatcgaca atccggcaca
    12661 caatctataa ctttttattt caaaaggaat aatggcaggc ttcgcccgca aatcgaaaat
    12721 ccttccccac cggtctcctg ccgccgcctt cccccgcgtc cgcccttttc ttgaaagcat
    12781 aagcgaatcg ggcgataatc aacgctttcc gattatccac ttatccgaaa caccagcaag
    12841 gaaaatacaa aatgtctcaa ctggcaaacg caatccgctt cctctcggcc gatgccgttc
    12901 aaaaagccaa ttccggccac cccggcgcgc ctatgggtat ggcggaaatg gcggaaacat
    12961 tgtggacgaa attcctcaat cacaatcccg ccaaccccaa attctacaac cgcgaccgct
    13021 tcgtcctctc caacggccac gcgtctatgc tgttgtacag cctgctgcac ctgaccggct
    13081 acaatctgag cattgaagac ttgaaaaact tccgccaact gcacagcaaa acccccggcc
    13141 atcccgaata cggctatacc gacggcgtgg aaaccacgac cggcccgttg gggcaaggga
    13201 ttgccaacgc ggtgggcatg gcattagccg aaaaaatcct tgccgccgaa tttaataaag
    13261 atggtttgaa catcgtcgat cattacacct acgtcttcat gggcgacggc tgcctgatgg
    13321 aaggcgtatc gcacgaagcc tgctcactcg ccggcacttt gggcttgggc aaactgattg
    13381 ttttatatga tgacaacaat atttccattg atggtaaagt ggacggctgg tttaccgaaa
    13441 acatcccgca acgctttgaa agctacggct ggcacgtcgt tcccaacgta aacggtcatg
    13501 acaccgccgc cattcaagcc gccatcgaag ccgcacgtgc cgaaaccggc aaaccgtcca
    13561 tcatctgctg taaaacctta atcggcaaag gcagtgccaa taaagaaggc agccacaaaa
    13621 cccacggcgc acctttgggc gcggacgaaa tcgaagccac gcgcaaacat ttgggctgga
    13681 cttaccctgc ctttgaaatc ccgcaagaaa tttacgatgc gtggaatgcc aaagaaaagg
    13741 gagcgaaact ggaagccgaa tggaacgaac tcttcgcgca atatcaagcc aaatatcctg
    13801 ccgaagccgc agaattcgtg cgccgtatgg ataaaaaact gccggacaat ttcgatgaat
    13861 acgttcaagc cgcattgaaa gaagtgtgcg ccaaagccga aaccatcgcc acccgcaaag
    13921 ccagccaaaa cagcatcgaa atcttggcaa aagagctgcc cgaactggtg ggcggctctg
    13981 ccgacctgac cccctccaac ctgaccgact ggtcaaacag cgtttccgtt acccgcgaca
    14041 aaggcggcaa ctacatccac tacggcgtgc gcgagttcgg tatgggcgcg attatgaacg
    14101 gtttggtatt gcacggcggc gtaaaaccct tcggcgcgac tttcctgatg ttcagcgaat
    14161 acgagcgcaa cgccctgcgt atggcggcac tgatgaaaat caaccctgta tttgtgttca
    14221 cccacgattc catcggtttg ggcgaagacg gcccgaccca ccagccgatt gagcaaaccg
    14281 ccaccctgcg cctgattccg aatatggacg tatggcggcc gtgcgacacc gccgaatcct
    14341 tggtcgcttg ggcagaagcc gtcaaagccg ccgatcaccc gtcctgcctg attttcagcc
    14401 gtcaaaacct gaaattccaa gcgcgcagcg aacaacaact ggacgacatc aaacacggcg
    14461 gctacatcat cagcgaagcc caaggcaacg cccaagccgt catcattgcc accggctcag
    14521 aagtcgagct ggctttggaa gcgcaaaaag ccctcgccgc gcaaaacatc gccgtgcgcg
    14581 tcgtttccat gccgtccacc aacgtattcg accgccaaga cgccgcctat caagccgccg
    14641 tcctgcccga aagcctgccg cgcatcgccg tagaagccgg ccacgccgac ggctggtaca
    14701 aatatgtcgg actgaacggc gcagtcgtcg gcatcaaccg cttcggcgaa tccgcccctg
    14761 ccgatttact cttcaaagca ttcggcttta ccgtggacaa tgtggtcgat acagtgaaat
    14821 ccgtgctgta accccacacc taaacaaatg ccgtctgaaa ccaattaggg cttcagacgg
    14881 catttgtttg ccctctctcc ttgatacttc ctcattcctc caatctccat tataatattt
    14941 gcgaatcact cttattcaca tttcaaaagg agaaacgcat gagcacccgt accgaacacg
    15001 acaccatggg caatgtcgaa gtcccgtccg aagcctattg gggcgcgcag acccagcgca
    15061 gccgcaacaa tttcaaaatc ggcggcgaaa ccctgccgca gccgctgatt tatgctttgg
    15121 cgttggtgaa aaaagccgct gccgcgacca atgtttccct cggcaggatt aagccggaac
    15181 aggcggattt gattacgcag gcggcggatg atgtgttgaa cggcaggctt gacggacagt
    15241 ttcctttagt ggtttggcag accggctccg gcacgcagtc caatatgaac atgaacgaag
    15301 tgctggcaaa ccgcgccaac gaaatcgccg gtacgggttt ggcggcttat cagcccgtcc
    15361 atcccaacga ccatgtgaac cacgcgcaat cgaccaacga cgcattcccg accgctatcc
    15421 acgttgccgc cgcgattgaa atcaaccgcc acctcatccc cgccgtaaaa gccctgcgcg
    15481 ataccttgga caaaaaagcc caagctttcg cccctatcgt caaaatcggc cgcacccact
    15541 tgcaagacgc gacgccgctg actttgggac aggaattttc cggctacgtt tcccagcttg
    15601 atcacggctt aggccgtctg aacgatgcgc ttaaagactt gtatgaactt gctttgggcg
    15661 gtacggcggt cggcacgggt ttgaacagcc atcccgaata cgccgaaaaa gctgctgcta
    15721 aactcgccga actgtccggc ttgccgtttg tcagcgcgcc gaacaaattt gaagccttag
    15781 gcggacgcga tgccgccgtt gccgcttcgg gcgcactgaa aacgctggcg gcaagcctga
    15841 ataaaatcgc caacgacatc cgctggctgg caagcggccc gcgctgcggt ttgggcgaaa
    15901 tcaaaatccc cgaaaacgag ccgggttcgt ccatcatgcc gggcaaagtc aatccaaccc
    15961 aatgcgaagc gatgaccatg gtgtgctgcc aagtgttcgg caacgacgtt accatcggta
    16021 tggcgggcgc gtcgggcaat ttcgagctga acgtctatat gcccgtcatc gcctacaacc
    16081 tcttgcaatc catccgcctg ttgggcgacg cgtgcaacag cttcaacgaa cactgcgccg
    16141 tcggcattga acccgtaccg gaaaaaatcg actatttcct gcaccattcc ctgatgctcg
    16201 ttaccgcgtt aaaccgcaaa atcggttacg aaaacgccgc caaagtcgcc aaaaccgcct
    16261 acaaaaacaa caaatcgttg cgcgaaaccg ccgttgagtt gggcttgctg acgggcgaag
    16321 aatttgacga actggtcgtt cctgccgata tggttcatcc gcgctaatct ttccctcaaa
    16381 taaaatgccg tctgaaacct cgttcggacg gcattttccg ttgcctgcga actagcggcg
    16441 tttgaacagc ctgtccccca ccgccgccgt aaccgcaccc ccgaccacga tcagtgcgcc
    16501 aacataaccc aaaccgttca tatccggcgc ggcaaaagta tcaggcatca cataatgccc
    16561 gagcaaagaa aatattacgg taaacacggg gagcaaggtt gttaccgcgc tgactttgga
    16621 agcctcccaa tgtttcaacg cctcgccgaa cgagccgtaa ccgattaacg tattcaagca
    16681 gcaatacgca aaacaaaccc acgccaacgt accgtccaaa cttccgatgt gtgccggttc
    16741 ggcaaacggc aggaacacgg cggcacttgc cgcataaatc aacagcagaa tctgttgcgg
    16801 cccgaattgc gccgacagca gcttttgcgc cacggcataa cacacccatg ccatactgcc
    16861 tgccgcacac agcaacacgc ccttcgcata cgcgcccaaa cccgacaact cgccgaattt
    16921 atcgttaaaa aacataagca aaccggcaag cagcaaaacc aagccgattt tctgagcggc
    16981 agtcatccgg tctttaaaca ccaacacacc gacaacaatc atcgtaaacg gcgaaatctg
    17041 ccacaaaacc tgcgtcgtgg tcggcgaaat ataatgcagc ccttgggcaa tcagcacaaa
    17101 gtttgccgaa atgcccgcca cgccgagcag cagcagcctg aatgagcacc aagaaaaatc
    17161 ccgccacttc ggcagccgcc cgcccaatgc cagcaaaaca aacaataccg ccgccgccac
    17221 ggtaaaacgc acccacacca gcgtcggcgc atcgacaaac ttcaatacct gccgcacggc
    17281 aatcggcagc gttccccacg tcatcgccgc caaaagtgcc aacgcgaagc ctaggagcgg
    17341 cctttggttt tccatcctga ttttcctatt tttaaacaac cgtattgccg gacgatgccg
    17401 gtttgccgca tcgggcaatg atggttcaag cgtttgacgt ttgattccaa ccctttgatt
    17461 tcaaacaaac cggctgaagc ccgactattg cttcgcgcta tttggaaaca ccgcctgaat
    17521 tttaaaatgc cgtctgaaaa cagcggggac gtgcggcagg catcactgcc tcaaaacgcc
    17581 cgaacggcgc aatcgcaacg ttccgcccaa cgcaaggggc ggcaacaagc cggctcggat
    17641 gcaaaaaaga gaaaccctgc cccgtaaggt ttaaggtttc tccgtccttt atgatttccc
    17701 tctgcgagga tgtccggccg taaaattcag aacgggatgt cgtcgtcaat atcctcgacc
    17761 ggggcggcgg caggcacggg ttggcggcgc ggcgcggctg gtgcttcttg gggatgggac
    17821 ggcgcgtcgg aggcgggctg ccggctttgc tgtgcggggc gttggtaagc ctcctgactc
    17881 tgaccgtaac cttcctcgta aggcgcaccg ccgctgtttt cattgcgccc gcccaacatt
    17941 ttcatttcgt tggcgacaat atcgtaagcg gtgcgttcga tgccgtcttt gccttggtat
    18001 ttgcggcttt ggattctgcc ttccagataa accagcccgc cttttttgag gtattgcccg
    18061 gcaatttccg ccagtttgcg gtacatcgtg atgttgtgcc actcagtacg ctctacacgt
    18121 tggccgttgc ggtcgttcca agtttcgctg gtggcgacgc tgaaattaca caccgcctcg
    18181 ccgttgggca tatagcgcac ttcgggatcg cgtccgaggc ggccgataag gataactttg
    18241 ttcaatgaca tttttaaact cctgtaatga ttttttcagc ggcagcctga tcgaaaccct
    18301 tctgcaacac tttgagatag acggtctgcc cgtcgaaact gaaaccgatg tcttccacgc
    18361 cttcgagttg caacagggcg cgttgcaatc cgtcccgatt gacctgccac acgccgccga
    18421 caggatagct gaggtttttg acgggcttgg gcgcgggcga taaaacggca attaccagcc
    18481 acagcagcat caatatactg caaaaggcaa acacgccgga aaagccgtat ttttgaaaca
    18541 gcaaaccgcc tgccgcgccg ccggcaaaca gtccgagcga ctgcatcgtg ttgtacacgc
    18601 ccatcgccgt acccttcagg tcggacggcg cgattttgga aaccatagac ggcaggctcg
    18661 cttccaacac attaaaaccg ataaagtaaa caaccaaata agcggtaatc aagcctaccg
    18721 agcgcatacc ggacagcaaa ccgagctgcg ccgccgcaat acaggcgata cccaagataa
    18781 aaacctgttt gagcttgttg cgcgtctcac cgacgataat cagcggaacc atcaccacca
    18841 atcccgtaat ggtcgagggc agatagactt tccagtgctg gattttttcc aaaccgagct
    18901 gggtcatcgc gaaaggcagc gcggtaaaca atgccatttg cgcggcgtgc agggcgaaaa
    18961 tgccgaaatc gagcgtcagc agcctacggt ttttcaaaac ttcgcctatg cgcgaaggct
    19021 gcgcctgcgt atcttcgtgc agcttggaaa cctccgggtc gggagtcatc cacgccacca
    19081 cgccgatact ggcaacggtc aaaataccgg tcagcaggaa caagccgcgt acgcccaccg
    19141 cgtcggcaat cacaggggca acgacgaggc tgaccgaaaa cgtcagaccg atactcaaac
    19201 cgatcatcgc cattgcgcgg gtacgtacgc cgtcgcgcgt caaatccgcc agcagcgcgg
    19261 taaccgccgc actgaccgct cccgcaccct gtatggcgcg tgcggcgacc agcatgggca
    19321 gcgtatcggc agcggcggca aggaaactgc ccgctgcaaa cacaatcagc ccgacataaa
    19381 tggttttctt gcgcccgaac ttgtcggaag cgatgcccaa aggcagttgc agcagagcct
    19441 gtgtcagccc gtaaatgccc attgccagcc cgaccagcgt tttgttgtct tccgcgccgg
    19501 gcagcgaggc ggcatacacc gccaatacgg gcagcacgag gaacataccc agcatacgca
    19561 gcgcgtacac gccggaaagc gtcgtactgg cgcgccattc gtgcggaaac atttggatgc
    19621 ggttatctct tgccatcata ttttttcaga cggcatcaac agttgcaatg ccgtctgaac
    19681 ttccagtgaa cagattttcg gattatacag gattcgccgt atttcggttg cggcgcgggt
    19741 tcaaaatcaa cgccactgcc agcggttgcg ccacgcgccc aaaacggcgt tcggatattt
    19801 attgctgccg aggctgccat taaagcgggc aagcgcgcgg acgatgttgc ctttttcaag
    19861 attccggtaa tggcgcagga tggtacagcc gtaacgcagg ttggtgcgga tgtcgaacag
    19921 gttgtgcgcc ggtttgccga tgtagttttt ccaaaacggc ataacctgca tcaggccgcg
    19981 cgcgccgaca ccgctgattg catactggcg gaacgcgctt tccacctcaa tcagccccaa
    20041 cacaatctgc gtatccaaac cggcccggct gctttcatac tgaatattga ccagcagcct
    20101 gcgccgctcc tcctcctcgg ggacgaacct tgccaaacgt gccgacatgg cagacaacca
    20161 acgttcgccc tctttcggat tgtcaaacac cagcctcggc ggattgacgc tgccgacaga
    20221 actcctcatc acggaagcca catcgtcggc aagcgtttcc tcacgttgcg cgccggcgtg
    20281 cgccagagga ctgagcaaca acgcaccggc ggcacacaac aggcggcggc gttgcagatt
    20341 aacgggtagg gtatcggtcg gttttttcat agggaacggg ggcgcgtccg gacgtttcag
    20401 acggcattaa atattcaaac agacataatt gcttccaacg cgaaaaaccg cgcacaaaat
    20461 ccaagcgcgg catatcgccc tgcccttttc gggcaaacct tgattttacc gccctcaaga
    20521 acgcttgtcc aaacaggcac aggcaacacc acccgggcat ttcccatttt cactggttat
    20581 ccgtcgtccg gattgtgcag cagcaccatc agcgcattac gcttttcggg cggcagcagg
    20641 cg
//
LOCUS       DAXHRI010000022        19756 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXHRI010000022 DAXHRI010000000
VERSION     DAXHRI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.022"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(203..278)
                     /locus_tag="ACFM75_000602"
     tRNA            complement(203..278)
                     /locus_tag="ACFM75_000602"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="ACFM75_000603"
     CDS             complement(371..730)
                     /locus_tag="ACFM75_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC6255189.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="ACFM75_000604"
     CDS             complement(753..1397)
                     /locus_tag="ACFM75_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC6255190.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADV
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="ACFM75_000605"
     CDS             complement(1399..1935)
                     /locus_tag="ACFM75_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC6255191.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="ACFM75_000606"
     CDS             complement(1947..3044)
                     /locus_tag="ACFM75_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6255192.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLASVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="ACFM75_000607"
     CDS             complement(3047..4483)
                     /locus_tag="ACFM75_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6255193.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="ACFM75_000608"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="ACFM75_000608"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC6255194.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="ACFM75_000609"
     CDS             complement(5473..6120)
                     /locus_tag="ACFM75_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC6255195.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="ACFM75_000610"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="ACFM75_000610"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC6255196.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            7495..8571
                     /locus_tag="ACFM75_000611"
     CDS             7495..8571
                     /locus_tag="ACFM75_000611"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC6255197.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8778..10244)
                     /locus_tag="ACFM75_000612"
     CDS             complement(8778..10244)
                     /locus_tag="ACFM75_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6255198.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10442..11365)
                     /gene="speB"
                     /locus_tag="ACFM75_000613"
     CDS             complement(10442..11365)
                     /gene="speB"
                     /locus_tag="ACFM75_000613"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC6255199.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11464..13356)
                     /gene="speA"
                     /locus_tag="ACFM75_000614"
     CDS             complement(11464..13356)
                     /gene="speA"
                     /locus_tag="ACFM75_000614"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC6255200.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTASLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13523..13807)
                     /locus_tag="ACFM75_000615"
     CDS             complement(13523..13807)
                     /locus_tag="ACFM75_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6255201.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="ACFM75_000616"
     CDS             complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="ACFM75_000616"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC6255202.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(15824..16525)
                     /locus_tag="ACFM75_000617"
     CDS             complement(15824..16525)
                     /locus_tag="ACFM75_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC6255203.1"
                     /translation="MTEPEAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16668..17816)
                     /locus_tag="ACFM75_000618"
     CDS             complement(16668..17816)
                     /locus_tag="ACFM75_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC6255204.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTVSAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17976..18239)
                     /gene="rpsT"
                     /locus_tag="ACFM75_000619"
     CDS             complement(17976..18239)
                     /gene="rpsT"
                     /locus_tag="ACFM75_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC6255205.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18542..19684
                     /locus_tag="ACFM75_000620"
     CDS             18542..19684
                     /locus_tag="ACFM75_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC6255206.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5069 a         5110 c         4592 g         4985 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gttgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaat aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caggtaagca gcttctcact gctgaccatt
     1261 tcgtccacat ccgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacacttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgccg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcttta accgcatcgt ctgccggaga cgaaccggtg ttaatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ttcatggtaa cccaagctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgtg ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcggctt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggtgtctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcatca
     4441 gcaataccgc cagtgccaaa ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg tgcactttgc ccgacggcat actgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt attaccttaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agtctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatgg gcgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg ccattagatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgccg tcaatttccc gaacggtgca acgcggtgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgaa tcaaatctgc
     5641 gcgcctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgtcaaa
     5701 cggacgggcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggcgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgttc gccttgatga ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcacg ccgcaaactg ccgcacttca tccattgtca tatctgccgt
     6181 caaagacaat cgtattctgg atgtaccttt tggtactgtc ggtggtctga tgggcaggca
     6241 ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tggacgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc gaccagtaaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacggaaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgcg gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccctcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgaccct aaactcggtt tgggcataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcgggtctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca
     8161 cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg caccggatcg
     8221 gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa atcgctggcg
     8281 tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac tgaggaaacc
     8341 aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag
     8401 gcacaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac cgaaccggct
     8461 gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc ggttgatgta
     8521 ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg aataaccttc
     8581 tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt tgtttggctt
     8641 ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat gccatctgaa
     8701 ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac agattggaaa
     8761 tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct gccgataacg
     8821 aaaaaaatca gaaaattgac tacgctcatg ataaaacccg ccttccacca ttctcccatt
     8881 gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg agtgagggtc
     8941 atcataatgt tggatgcggc cgccatcatc agcgcggtcg gcatcgccgg ggcattcagt
     9001 gaaacggcag cagcgaaaaa tgcgccgaac atagcggtaa tatgtgcagt agtactggca
     9061 aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc cgtgccgcta
     9121 acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat cagtccgagt
     9181 ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt atcccacgcg
     9241 cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag caggcttaat
     9301 ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt aataagggcg
     9361 ggaacatctg cccacaacag caacaagata ccgaaaatga ccgccataat gatttcgtct
     9421 gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc attgggcgtt
     9481 tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa aaaggcgata
     9541 acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa actactgcct
     9601 aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt aataaacata
     9661 gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc catcttgcct
     9721 tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt
     9781 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag cagttcggaa
     9841 agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat aaacaaatat
     9901 ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac tgcgatggca
     9961 atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc cgcgcccggt
    10021 ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcgccc
    10081 aacggcatgg ccttgccgat aatggcggca atcacaccga caaacatggc cagcagcgtc
    10141 caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccagggc gcacaatact
    10201 gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac ctccgtaaaa
    10261 aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata agatatattc
    10321 agatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca gtatttgaat
    10381 tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg atgccggacg
    10441 ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg gcgccggcca
    10501 aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc ataccgacga
    10561 tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc aagccgccgc
    10621 atacgggcgt accggtcccg ggggcgaacg acgggtcgag gcagtctatg tcgaaagtca
    10681 ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct tcaacactgt
    10741 cttcattgac ttggggggcg gtcaacacag taaaaggcaa ttttttactg tgttcggtac
    10801 gtatgccgat ttgtacggaa cgggacgggt cgatgaggcc ttccttgggg gcggtataga
    10861 acatcgtacc gtggtcgtat tcgctgccgt tgtcgtaggt gtcggtgtgc gcgtcaaaat
    10921 gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggg agggtaatga
    10981 aatggtcgcc gcccaaactc aaacagcgtt tgccggaaga aagtaatttg ccggcgtgcg
    11041 cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaag tcgccgcaat
    11101 caataatgtt caggcgttcg cgcacatcaa atgtccatgg aaacctgcgg tgctcccaag
    11161 cgaggttgac ggaggcgcgc cggatggctt caggaccgaa acgcgcgccg gaacgccctg
    11221 aaaccgccat atcataaggc acgccggtaa taacccaatc ggcatgactt tcatacggca
    11281 taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagtgag ttgtcggttt
    11341 gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt caatcggtcg
    11401 tgatgattgg tgtttgagta agaaaatcgg gcttcagacg acatatccga tgccttgatg
    11461 cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt cttttaagaa
    11521 agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat agcgtttcat
    11581 cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt ttccttcatc
    11641 gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcggcag tggcagtgtc
    11701 gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca taaaaaagcc
    11761 taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg cgatgccgtc
    11821 gccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc
    11881 gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa gttgatctat
    11941 gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct tatcggcaaa
    12001 acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat taaacaattc
    12061 gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact
    12121 caacagcccg acattatact gattatgcac atcggcaaga tcaaactgcc cttcgtgtat
    12181 ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag tttcccacat
    12241 actgtgcaac acacgcggcg cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg
    12301 ttcaacgcct ataacattgg caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct
    12361 ctcggtgatg attgtcggat gcggcagccc gtgttcgaga caagcctgac tgatgcccca
    12421 tacgactgtg gcggcatatt cgttgaggct gtaattaacg gaacaatccg attgtgtgcg
    12481 gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga tatttacccc
    12541 cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg caacatcacg
    12601 gatgttccca agctgcgagc ccaaatggaa atgcaaaagc tgcaggcaat ccagcctgtt
    12661 tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc cgaattttga
    12721 tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc tggcgcgcac
    12781 acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt gtatttcgga
    12841 cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca aggcgaaacg
    12901 gatatattca cggtctttat agccgttgca gacgattaat gtttgccggg tgccggcgtg
    12961 tgccaaaacc gccatcagtt cggctttaga accagcttcc aaaccatgcg gttgtccgct
    13021 tgacataagc gattcgatga cgcggcggtg ttggttgacc ttgatagggt aaaccaaaca
    13081 ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt taatgtcgcg
    13141 gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt
    13201 ttgttgcacg gcttcagtca gtttttgcag tgaagcagtt tgattgtgtt gcgccggatt
    13261 ggggcggacg atgatttcgc cggaatcgtc aacatcataa taacctatgc cccaatgatt
    13321 aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc gtctgtcgtg
    13381 ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat aagaaaagcc
    13441 tgcctcatac ctgatgagga acaggcaaaa tgccgtctga acgcttcaga cggcattttg
    13501 gttcagcttg ccatcaaagg agtcagcgat cgagctgctc tttgatgatt ttcaggtcgg
    13561 ggtaagacaa cacgatgtcg tattcgcggc cgtctttata ggcttccact tccagcacag
    13621 gcttacccca atggtcgtcg gcatcgacat cgtgaacctg ataaccacgc tgttccaaca
    13681 ttttcacggc ttttgtgcgg ttttgttcaa aatggggatc gccgtaaatc tgacgctcgg
    13741 cagaatcgcc ggcaaatgcg gttgcggcac tcagggaaac aacggcggcc aataacagtt
    13801 ttttcatttt cagtcctttt tttatcggtt gattgaacaa gatgtgtttt tcaataccgc
    13861 cattaaaaca cagcaaaatt aggtttgaat tagagggaag tcaagaagcg taaatgccgt
    13921 ctgaaaaaaa cagagccgtc aaacggctct gttttcctta tgcttctttg gtttcagcca
    13981 ccttttggcg caggcgcagg ctcaattcac gcaactgctt gtcgtccacg ctgttgggcg
    14041 cgttggtcag caggcattgt gcgcgttgtg ttttcgggaa ggcaatcacg tcgcggatgg
    14101 attcggctcc ggtcatcagc gttaccagac ggtcgaggcc gaatgcaagg ccgccgtgag
    14161 gaggcgcacc gaatttcagg ttgtccaaga ggaagccgaa tttctcttgt tgctcttcag
    14221 ggctgatttt cagcgcggca aacactttct cttgcacgtc tgcgcggtgg atacggatag
    14281 agccgccgcc gatttcccag ccgttcaata ccatatcgta ggcgcgtgcc aaacaatttg
    14341 ccgggtcgga aaccatcagg tcttcatgac cttcttttgg cgcagtaaac ggatggtgta
    14401 cggcaacgta gcggtcggct tcttcgtcgt attcaaacat cgggaagtcg acaacccaca
    14461 aaggtttcca ttcgtctgtg aagtagccgt tttccgcgcc gtgttctaaa ccgactttaa
    14521 tacgcaatgc gccgatggct tcgttcacaa ctttggtttt gtctgcaccg aagaagataa
    14581 tatcgccgtt ttgtgcgccg gtacgctcga tgatttcttt cagggcattc tcagacagga
    14641 attttacgat tggagattgc aggccgctgt cttcgccgtt ggaaaggttg cccacatcgt
    14701 ttactttgat atatgccaga cctttcgcac cgtagatgcc gacaaattta gtgtattcgt
    14761 cgatttcttt gcggctgaat ttcgcaccgt tcggcacacg cagggcaacc acgcggccgc
    14821 ctttcatgtc ggctgcgcca cggaagactt tgaattcttc cgttttcatc aggtcggtca
    14881 actcggtgaa tttcaagttg atacgcatgt ccggtttgtc agagccgtag tagaacatgg
    14941 cttcagagta aggcatgcgt gggaagtcgc ccaagtccac gcccagcgcg tctttgaaga
    15001 cttgtttcgc cataccttcg gtgatgtcca tgatttcatc ctcgtttaag aacgaggttt
    15061 ccaagtcgat ttgcgtgaat tcaggctgac ggtcggcacg caagtcttcg tcgcggaagc
    15121 acttggtgat ttggtagtaa cggtcgaaac ccgccaccat caacagttgt ttgaataatt
    15181 gcggcgattg aggcagcgcg aaaaactcgc ccggatgaac gcggctcggc acgaggtagt
    15241 cgcgcgcgcc ttcaggcgtg gagcgggtca gcatcggggt ttcaatgtcg atgaaacctt
    15301 gcgcgtctag ataacggcga acgcccatag cgacttggta acgcaggcgc aggttgcgct
    15361 gcatcacagg gcggcgcagg tcgataacgc ggttggtcag gcgaacgttt tcgctgatgt
    15421 tttcatcgtc gatttggaac ggcggcgtgg cggcggcgtt caacacttcg atttctttgg
    15481 caaggatttc gattttgccg gaaatcattt tatcgttggt cgtgccttcg ggacggttgc
    15541 gtacgcggcc ggtaatgctc aaaacgtatt cgttgcggga ggaatcggca gcggcaaacg
    15601 cttcgggcgt gtcgggatcg atcacgactt ggacgatgcc ttcgcggtcg cgcaggtcga
    15661 taaaaatcac accgccgtgg tcgcgtcgac ggtgtaccca gcctttgacg gtaacggttt
    15721 ggtctaagta ttgctcgcta atcaggccgc aatagttggt acgcataaaa tcacctttta
    15781 ttgatttaaa ctgaaaacag aaaatgccgt ctgaacggcg gctttattgt tgttcgggca
    15841 aatccgcctt ttcagacggc ataggtcctg ccaatgtttt gacgggcagg tcgtcaggga
    15901 tgaccatacc cagcgaaatc acatatttca acgcttcgtc cacgctcata tcgagttcgc
    15961 gcacatcgct tttctttacc ataatatagt aaccgccggt cggattcggc gtggtcggaa
    16021 catacacgga aagataatcg ccgtccttcg gcaatgcggc cttaaccgca ttcgacacct
    16081 gaccggacac gaatgcgatt gtccaaatac ccgattgggg aaacggcacg agtactggtg
    16141 ttttaaacga acggctgctg tcggacagca gcgattcgga tacttttttc acactcgaat
    16201 agatggactt cacaactgga atccgcccca acaggctgtc ccacgcggca agaatctgcc
    16261 ggcccaatac gtttgcggca aacaatccgg ttacaaacaa tacagcaatg gcaacgataa
    16321 cgcccagccc cgggatattg aagcccaaaa catattgcgg ccgccattgc ttcggcagca
    16381 ggttgacgag ctgatcggac gcggaaacga tataggaaac cacccaaacc gttaccgcaa
    16441 tcggcagcca gaccaaaatg cccgtaatca gatatttttt taacgccttg gcagctttac
    16501 cgccttcggc ctcaggttcc gtcatcttgc ttgattccga caaagtccgt acaaaccgca
    16561 cattatacgc gtttgcccgg attcaaacga aatttttatc ccgccccgcc aaaccgccag
    16621 cggcttcaga cggcacggca acttgatata ccgtctgaac acgcggttca gatgccgtcc
    16681 aagtcgttga acatcaaccc gataccgata ccgttctgct tgtggttgta gtcgatcagg
    16741 ctctcgccgt aaccgtggaa tccgcgtacc acgcctttga gtttgccctt aatcggaaac
    16801 gtgtaggcgg cttcaatcgc gccgtagccc gttttggggt tgtagcgcaa tacggaatac
    16861 acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc catatagtcg
    16921 gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac ccacacgcgc
    16981 ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cgtaaatcct gttccacgaa
    17041 cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgcacc gagcatacgc
    17101 agcctgccgc cgaacggcaa atccgccttc acaggctggg tcaggaaaat ttcaggtttg
    17161 taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg ccaatcggat
    17221 ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc ggcaattttg
    17281 cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa tttttcctgt
    17341 acagtcgtac cgcgcgtcgg cgaacccggg gcatagttgg gcgaattgtt gtaccagagc
    17401 ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa atcgtttttg
    17461 tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc cgcactgtcg
    17521 gcaggaagcg catccccgcc tttttcaaca acaatgaccg cctcgccctt atccaggctg
    17581 ctgcggacgg tttccgtcag attgagtacg gctttcgact cctgcccttc ctgccctgcc
    17641 gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt aacattgtcc
    17701 gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg ataccgtcgg caacagtcct
    17761 gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt cggcatattg
    17821 gttttcaaac ggcattttat agcggattcg gataaaaacc gcaccctttc cgccatttcg
    17881 ggattttgcc cccgcaatac agaaaacccc gaaaccgtcg ggcttcaggg ttttccgctt
    17941 atcgcgtatc aaccgccttg gcggttttgc aaaaatcaag ccagtgcttt tacttttgca
    18001 gacagacggc ttttgtggcg tgccgctttg tttttgtgga acacgccctt gtcggcgatg
    18061 cggtcgatga ctttgacgga ctcttggtaa accgcttgtg cggcagcttt atcgcctgct
    18121 tcgactgctt tcaatacttt tttcactgca gtgcggaatg cggtacgcag gctagcgttg
    18181 tgggcgcgtt gtttgacgga ctggcgggca cgtttgcgtg cttgtgcgct gtttgccata
    18241 ttggaatctc ctgaaagaaa aataaattct gtaaacgcgc aattttaaag ggggtttaca
    18301 ttatatgcaa gccttttctc tctatccgcc cgtcggacac cctgacagcg caaaaaaacg
    18361 gcaaaatcgt gcggacgttt tctccctctt ttgaaaacaa ggtaattttt taggaaaatc
    18421 aggtcaatat cgctttgagt ttgcacaaat ttactttaca atgcccaaac cttgcgccgg
    18481 ggtgtttatg gggcgcgggg ttttcttttt ttccaacctc tttcatcagg aatctgcccg
    18541 tatgaaaaaa tccgtattag ccgttttggc cgcgctgtct ctggcagcct gcggcggcag
    18601 cgaaaaaaac gccgtgcaac ctcaagcaga cgctgcgtct gctgccaatg ccgaagccgc
    18661 tgccaccgat actctgaaca tctacaactg gtcgaactat gttgatgaga gtacggtcga
    18721 agactttaaa aaagccaaca atctgaagct cacttacgac ttgtacgaaa acaatgaaac
    18781 gctggaagcc aaaatgctga ccggcaaatc cggttacgat ttggttgtgc cgggcatcgc
    18841 cttcctaccg cgccaaattg aggcgggcgc gtatcaaaaa gtcaacaaag acctgattcc
    18901 caactataaa aacatcgatc ccgaactctt gaaaatgctg gaaaccgccg acccgggcaa
    18961 ccagtatgcc gtcccctatt tctccggcgt gaacacgatt gcgattacgg cgaaaggcaa
    19021 agagctttta ggcggcaagc tgcccgaaaa cggctgggat ttgctgttca aacctgaata
    19081 cacccacaaa ctgaaatcct gcggcatcgc cctgtgggac accccgagcg aaatgttccc
    19141 gattttgctg aactacttgg gcaaagaccc caaaggctcg aatcctgaag atttgaaggc
    19201 ggcggcggaa gtgttgaagt ctatccgtcc ggatgtcaaa cgtttcagcc cgtccatcat
    19261 cgacgagctg gcgcgcggcg acatctgcct ggcggcaggc aacggcggcg atttgaactt
    19321 ggcgaaagca cgttccgaag aagtgaaaaa caacgtcggc atcgaagtgc tgaatccgaa
    19381 aggcatgggc ttctggattg agtcttggct gattcccgcc gatgcgaaaa acgtcgccaa
    19441 tgcccacaaa tacatcaact acacgctcga ccccgaaatc gcggcgaaaa acggcatcgc
    19501 cgtaaccttc gcccccgcca gcaaaccggc gcgcgaaaaa atgcctgccg agctggtgaa
    19561 cacccgttcc atcttcccga acgagcagga tatgaaagac ggtttcgtga tgccgcaaat
    19621 gagtacggat gcgaaaaaac tgtctgtcag cctgtggcag aaaatcaaag tcggcaccaa
    19681 ctgatttgaa gcattaaaaa tgccgtccga acgatgttcg gacggcattt tatattggat
    19741 tgaaatagaa atattt
//
LOCUS       DAXHRI010000023        19503 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXHRI010000023 DAXHRI010000000
VERSION     DAXHRI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19503
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.023"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(79..771)
                     /locus_tag="ACFM75_000621"
     CDS             complement(79..771)
                     /locus_tag="ACFM75_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6255207.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1077..1634)
                     /locus_tag="ACFM75_000622"
     CDS             complement(1077..1634)
                     /locus_tag="ACFM75_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255208.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLEIWHGDRVFMH"
     gene            complement(1879..2037)
                     /locus_tag="ACFM75_000623"
     CDS             complement(1879..2037)
                     /locus_tag="ACFM75_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255209.1"
                     /translation="MKTLLLICTLSALAACALSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2034..2876)
                     /locus_tag="ACFM75_000624"
     CDS             complement(2034..2876)
                     /locus_tag="ACFM75_000624"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6255210.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETRVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            2942..3082
                     /locus_tag="ACFM75_000625"
     CDS             2942..3082
                     /locus_tag="ACFM75_000625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255211.1"
                     /translation="MPSEKYGIRAVKLCGGKCAIIRAVLPLLRSCSANIRAAWTYNRI
                     PA"
     gene            3111..4031
                     /gene="pyrB"
                     /locus_tag="ACFM75_000626"
     CDS             3111..4031
                     /gene="pyrB"
                     /locus_tag="ACFM75_000626"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC6255212.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEVGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4041..4499
                     /gene="pyrI"
                     /locus_tag="ACFM75_000627"
     CDS             4041..4499
                     /gene="pyrI"
                     /locus_tag="ACFM75_000627"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC6255213.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4564..5229
                     /locus_tag="ACFM75_000628"
     CDS             4564..5229
                     /locus_tag="ACFM75_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255214.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5292..6509)
                     /locus_tag="ACFM75_000629"
     CDS             complement(5292..6509)
                     /locus_tag="ACFM75_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6255215.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6693..7196
                     /gene="def"
                     /locus_tag="ACFM75_000630"
     CDS             6693..7196
                     /gene="def"
                     /locus_tag="ACFM75_000630"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC6255216.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            7283..8209
                     /gene="fmt"
                     /locus_tag="ACFM75_000631"
     CDS             7283..8209
                     /gene="fmt"
                     /locus_tag="ACFM75_000631"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC6255217.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8288..9547
                     /gene="rsmB"
                     /locus_tag="ACFM75_000632"
     CDS             8288..9547
                     /gene="rsmB"
                     /locus_tag="ACFM75_000632"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC6255218.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9513..10112
                     /locus_tag="ACFM75_000633"
     CDS             9513..10112
                     /locus_tag="ACFM75_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC6255219.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10112..12232
                     /locus_tag="ACFM75_000634"
     CDS             10112..12232
                     /locus_tag="ACFM75_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6255220.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQQAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRKREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            12225..13502
                     /locus_tag="ACFM75_000635"
     CDS             12225..13502
                     /locus_tag="ACFM75_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC6255221.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13593..14780
                     /gene="dprA"
                     /locus_tag="ACFM75_000636"
     CDS             13593..14780
                     /gene="dprA"
                     /locus_tag="ACFM75_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC6255222.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGSRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14806..15267
                     /locus_tag="ACFM75_000637"
     CDS             14806..15267
                     /locus_tag="ACFM75_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC6255223.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15339..17645
                     /gene="topA"
                     /locus_tag="ACFM75_000638"
     CDS             15339..17645
                     /gene="topA"
                     /locus_tag="ACFM75_000638"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC6255224.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17718..18413)
                     /locus_tag="ACFM75_000639"
     CDS             complement(17718..18413)
                     /locus_tag="ACFM75_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255225.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(18433..18984)
                     /locus_tag="ACFM75_000640"
     CDS             complement(18433..18984)
                     /locus_tag="ACFM75_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255226.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIVFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19103..19480
                     /locus_tag="ACFM75_000641"
     CDS             19103..19480
                     /locus_tag="ACFM75_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC6255227.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
BASE COUNT         4839 a         4986 c         5352 g         4326 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat ataaaataaa
       61 tgggcaaaaa tcggtttatt atcgtttttg ccgcatttgg atttgttcta ccgtaaaacg
      121 tgtttgacga acgggattct tattaaaaaa catctgattt ctaacaaaat cagtattttt
      181 tggcacgatg gctaaaattt ttccttccat ttcgccatca cgtgttttcc atgcgctcaa
      241 gaattgtgat ttgctcattg agacgtgccc cagcgatgga tcagccagca aaacagtttc
      301 tccgttaata ccgttcaata ccgaaaaatg gttgttttta cggtatttta aatacacaat
      361 tacaggaatt tttagttgta ccaactgttc aaatggcaaa gcataacctt gtgcttcaaa
      421 acccagttcg ggcattatgc gttgcatatc atcaaaagaa gcacgcattt gggttttatc
      481 cattttgtcc aagatttccg cttcagaata atgtctgcca taaaaattat tcagtaacgt
      541 ggcaatcgaa gccgcgccgc aagaaaaatc caaatcttgt tttactatgc cggaatctcg
      601 ccgtgctttc caactccgta catggatgtt ttggtaagaa gcggggtttt cagcaaatat
      661 agggtaagca aaaacaatat ttgggataaa gccaattaaa accgcatggt ttattaattt
      721 agaaatattt tttatcatag tatatatgcg tagaataaac gcatgaaaca taaaaaaata
      781 aaatcatatg caattttatt gcatgaataa atatgaataa aatagataat gatgggagta
      841 aatacgccat gtattttgga agtttaaatt tattaataat aaaataattt atcgtagcgc
      901 aaataaatcc caaaattgga acgattagaa aaaaaattaa tacacccatc atcatgcaaa
      961 ctctatatta ataaatagct aactaattct attgtggaaa ttagttagct aactaaaagt
     1021 tattaatgat tatttccgag aattgactgc attgttggca gcattggccc caaaacctag
     1081 tgcatgaata cccggtctcc atgccaaatt tccagccaat ccgcctccgg cagcagcagc
     1141 cagccctgtt ttgccgctac tcctgttgcc gcaccgattc ctgccgcagt agccgcgcct
     1201 tgcgcagttc ctaatttacc atgattatac aaattagcac catgatactc ccatgcacct
     1261 aatgcaccgc caaaagcagc ggctgcaata atgggaacaa attcaccttg tgtttctttc
     1321 atttcagcct gtgataattg aattgctttc acattttggc tgtcaaaaac ttggctgtct
     1381 aaattttgcg ccattacagg tgtaatcatc atagccatta cagttgcaat tttcgttgcg
     1441 ctggtttgca cataaatagg attagcaaat tcgctttgat tgcgttcagt gttgatgtag
     1501 ctaatactgc tttctagttt gaatttaccc ttgtcagtca ataattcttc caaacttaaa
     1561 ggtaagtcag cataagcaat ttggctggca acagtcaaaa taaaatctat tagacatttg
     1621 tgtttttgca tcatttcgtt tgattttcta gtttttgaga atgatacaaa gtttttttac
     1681 aaagtaaaga gtcactctga aaaacatttt ttcattataa atcaaaatat tgatagaata
     1741 aatagcgagc atcgattcac ggtgtacttt agtgcaaagc gtacaacgcg agcctgaacc
     1801 accagccgca acaggaaaag aaagccgata gagtgcaatg cttgccaacg tgcaagcgag
     1861 cttgcaagaa cgcttggctc aacgagagca ggcaagacag aaagcagaaa gcaggatagg
     1921 agcggtaacg caaaggtctc gggctttgat ttcgccgtaa accctgctgc cgccttgtcc
     1981 ggaaagggcg caggcggcga gtgccgacag ggtgcagatg aggaggagtg ttttcatttg
     2041 gggtcgcaac ggaagtggta tgcgcagatt tcaaaaccgt ttttgaaata caggcggtgc
     2101 gcgtcggcac ggtcgtggtt gacgtggacg ttgaggtgga ttttggttac ccttgtttcc
     2161 gcgccgattt tgcggacttc ttccaaaagg cgcgaggcgt agcctttgcg gcggctttgc
     2221 ggcagggtaa cgatgtcgtc gatatggata tgtcggccgc cggcgagggt gcaggcttcg
     2281 cggaagccgc agacggcgac ggcgttgtgt ttgccttctt caaaaatacc cagcaggcgg
     2341 tagccttggg ggcgttggac tttgttgatc tgttcggtaa agcggttgat gtcggtcagg
     2401 gcggaacgca aaacgctcaa ggctgcaaag gcggtggcgg tgtcgtccgc gccgatttcg
     2461 cgcaaaacgt aggatgcgcc cgaggcggtc tgttcctgtg ctttctcggc ggcgtgtttt
     2521 tcttcgattg cctgtgccag catgacgtgt tcgtcggcag ggttgttttg tccgccctgt
     2581 tcgcgttctt cgagcagggc tttgcagtcg atgacgcgca ggtcgttgtc ggcggcaaag
     2641 tccatcagga agcggaacat ttggggattg tcggtttcca gttttttatc gacggcgacg
     2701 cagcggatgt tgtccaccag tatggggcgc acccatttcg agtaggaaag cctgtggtct
     2761 ttggtgaacg aggacagaat gccgcacagg cgttccgccc agtcgctggg acggaaaatc
     2821 ttgccggaac tcgttgtgcc gtggatgacg acttcgtagg ggttgcagac taacatggcg
     2881 gcttcctgaa aagaaatgtc tggctcgatt ataccttatg cttatgcagg cgtgtttgga
     2941 tatgccgtct gaaaagtacg ggattcgtgc ggtaaaactt tgcggcggca aatgtgcgat
     3001 aatacgcgcc gtattgccgc ttttgcgaag ctgttccgca aacatacggg cggcgtggac
     3061 gtataaccgg atacccgcct gacgcgggtt ttttacggaa gggggcaaaa atgcctaatc
     3121 cgctttacag acagcacatc atctcgattt cggatttgtc gcgcgaacag ttggaatgcc
     3181 tgcttcagac ggcattgaag ctgaaggcgc atccgcgcgg cgatttgttg gaaggcaaac
     3241 ttatcggttc gtgtttcttt gaaccgtcca cgcgcacgag gctgtcgttt gaaacggcgg
     3301 tgcagcggct gggcggcaag gtcatcggct tttcggacgg cgcgaatacc agtgccaaaa
     3361 aaggcgagac gcttgccgat accgcccgca tcatttccgg ctacaccgac gcaatcatcc
     3421 aacgccaccc caaagacggc gcggcgcgcg tggcagcgga gttttcgcgc gtccccgtca
     3481 tcaacgccgg cgacggcacc aaccagcacc ccagccagac cttgttggat ctggtaacga
     3541 tttatgaaac gcaggggcgt ttggacaagc tcaaaatcgc catggcgggc gacttgaaat
     3601 acgggcgcac cgtgcattcg ctttgtcagg cgttgaaacg ctggggctgc gaatttgcct
     3661 ttgtttcgcc gcccagcctg gccatgcccg actacattac cgaagagctg gacgaagtcg
     3721 gctgccgata ccgtatcctc ggcagtttgg aagaggcggc ggaatgggcg gatatcctgt
     3781 atatgacccg cgtccagcgc gaacgtttcg acgaacagga atttgccaaa atccaaggca
     3841 aattcaacct cgaagcgtct atgcttgccc gcgccaaacc gaacctgcgc gtgctgcacc
     3901 ctttgccgcg cgtggacgaa atccatcccg atgtcgatgc cacgccgcat gcctattatt
     3961 tcgagcaggc gaccaacggc gtttatgcgc gtatggcgat attgtcgctg gtgttgaacg
     4021 aagaagtgtg aggaaccgat atggaaaccc cgaaactcag tgtcgaagcc attgaaaaag
     4081 gtacggttat cgaccatatt cccgccggca gggggctgac catcctgcgc cagttcaaac
     4141 ttttgcacta cggcaacgca gtaaccgtgg gcttcaacct gcccagcaaa acccaaggca
     4201 gcaaagacat catcaaaatc aaaggcgtgt gcttggacga caaagccgcc gaccgcctcg
     4261 ccctgttcgc ccccgaagcg gtggtcaata ccatcgacaa tttcaaggtc gtgcagaagc
     4321 ggcatttgaa cctgcccgat gaaattgccg aagtgttccg ttgcccgaac acgaattgcg
     4381 ccggccacgg cgagccggtc aaaagccggt tttatgttaa aaaacacaac gggcagacgc
     4441 ggctgaaatg ccattactgc gaaaaaacct acagccggga ttcggtggcg gaagcctgac
     4501 ggattccctt aaaccgagcg ggcggcattt cgtctgccgc ttgttttgcc aatctgaaat
     4561 ggaatgatga tgcacgcttc tgtccaaagc cgtttcgcac cgatacttta tgttttgatt
     4621 ttctttgccg gttttttgac cgcgcaaatc tggttcaatc agaaagccta tactgaagag
     4681 ctgcctccgc ttctgtccgc attgtccgct gtcgcgctgg tgtggctggc gtgggcgttc
     4741 gtgtcggcgc gttcaaaggc taaggcggaa aagttctacc gcgaaaaaat gatacagaac
     4801 gaaagcatac atcccgtcct gcacgcttct ttgcaacact tggaacacaa gccgcaaatg
     4861 ctcgccctgc tggtcaaaaa ccacggcaaa gggatggcgg aacaggtcag gttcaaggcg
     4921 gaagtgctgc ccgacgacga agacgcgcgc acgattgccg ccgagttggc aaaaatggat
     4981 atgtttgcat tggggacgga cgcggtcgcc tcgggcgaaa cctatggacg cgtgttcgcc
     5041 gatattttcg agttgtcggc ggctttggaa gggcgcgcgt tcaaaggaat gttgaaactg
     5101 acggcggaat ataaaaacat cttcggcgat gcctgccgtt cggaaacggc gttggagttg
     5161 ggcgcgctca atcaggcgtt gcaggagatt tcaaaaacat cggaaaagtc caaacggata
     5221 ttttattgaa gatggaaaaa tgccgtctga aacggaaggt gtttcagacg gcatttttgt
     5281 cggatgatta attattcgga gcggttgaag ccaaacttca cgcggctgcg gccctgatcc
     5341 ggtatattgt ccaaatcgcg tcccggattg gcggcggtgt cgcctacgga aatatcggag
     5401 atgttttcca aaatgatggc ggacgacagg tgttcggagg tgcggtaaac cattgccaag
     5461 cccacttctt cggcaggagt ggaaatcagc tcgacggtat ccctgctttt gaaattgttg
     5521 gagaggtcga cctgcatcgt tttcttgcgt ttgtagaggc tcaaaaccgt gcctttgtcc
     5581 aaaccgtccg cctcgccttt gtcgatggtg atggtttgaa actggccggc aatccttgtg
     5641 ccttcaaaca cggaaacgat tttagcctga accgggcggg acggttcgtg cggcatcatg
     5701 ttgaagcggt cggtgtcttc cggcattttc atcaggtagt cgccctgctg tatttcggaa
     5761 atggcggttt cgaccaccag cggctgtatc gaaggggtgc gcagcggggt aatcaaagga
     5821 tgggtgcggg tatggtattc gttgtctttc ggccgttctc cagcctgttt cgagcgttgt
     5881 tcgaggacag agtcggtata gtcgagggag cgcacgatgc cgctgaatgc gacttcctgc
     5941 ccgaggaatt tacccgtatc cggatcggtg atgtttttat tgattcggta ggtcaggtag
     6001 cggcccggct ctttcaggcc tttggtgtaa accctggtgc ctttggtgta cagcagcctg
     6061 ccttccgggc ccgagagcag gcgcggcgcg gcagcggttt ctttgcggga aacgatttgc
     6121 gggtgccgca taaagatgcg gtagaagttg acatcgatgg cgggaatacc gtatccggac
     6181 acttccttat ccggactcat tttgacgacg gggatgccgt ctgtctgttc caagccgagg
     6241 cgcggttcgc cgtcaacgtg gcgcaacacc aatacctggt ccggataaat caggtcggga
     6301 ttgtggattt gatcccggtt cgcgtcccac aggcggcccc attgccacgg gctgtacagg
     6361 tatttgcccg aaatgcccca cagggtgtcg ccctgtttga ccgtgtagcg ttccggcgcg
     6421 ttcgggcgca cctccaaatt tgccgccaaa gtttgtgttg agaatgccat acctgccgcg
     6481 cagagcaggg ttataatacg acgttgcata accgttcccc ttatctgata aatttcggtt
     6541 tgtcttgctt gattgggttg gaaaaagcgg cggcagcccc tcgggatgtg ccgcgtgata
     6601 aaaaatgttc cgcattttaa catcgaatta tccgcaccat cacggtaatt atgaaaaaca
     6661 ggcggcgtat ccgccgaagg aaagagaaaa ttatggcttt attgaatatc ttgcaatatc
     6721 ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca agtcgacgag cgcatccgga
     6781 agctgattgc cgatatgttt gaaacgatgt acgaatcgcg cggcatcggg ctggcggcga
     6841 cgcaggtcga tgtgcacgag cgcgtggtcg tgatggattt gaccgaagac cgcagcgaac
     6901 cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg cgaaaccact tacgaagagg
     6961 gctgcctgtc cgtgccgggc atttacgaca ccgtaacccg cgccgaacgc gtcaaggtcg
     7021 aggctttgaa cgaaaaaggc gaaaagttca cgctggaggc ggacggcttg ctggcgattt
     7081 gcgtgcagca cgagttggat cacctgatgg gcatcgtgtt tgtcgaacgc ctgtcgcagc
     7141 tcaagcaggg acgcatcaaa accaagctga aaaaacgtca gaaacatacg atttgaccct
     7201 tttgccgtgc cgtctgaacg ctgcaaagtt ttcagacggc acggtcttgt ccgacaattt
     7261 tacgcacacg caggaacacg ctatgaaagt catcttcgcc ggcacgcccg attttgccgc
     7321 cgccgcctta agagccgttg ccgccgccgg ttttgaaatc ccgctggtgc tgacccagcc
     7381 cgaccgtccg aaagggcgcg gtatgcaact gactgccccg cccgtcaaac aagccgcgct
     7441 ggaactcggt ttgcgcgtgg cacagcccga aaaactgcgt aacaacgccg aagccctgca
     7501 aatgctcaaa gaggtcgagg cagacgtaat ggtggttgcc gcctacggtt tgattctgcc
     7561 gcaggaagtg ttggatacgc cgaaacacgg ctgcctcaac atccacgctt cgctgttacc
     7621 ccgttggcgt ggcgcggcgc cgattcaacg cgcgattgaa gccggcgatg ccgagacagg
     7681 cgtgtgtatt atgcagatgg acatcggttt ggacaccggc gatgtggtca gcgaacaccg
     7741 ctacgccatc caaccgaccg ataccgccaa cgaagtccac gacgcgctga tggaaatcgg
     7801 tgcggcggcg gttgttgccg atttgcaaca gcttcaaagc aaaggccgtc tgaacgcggt
     7861 caaacagccc gaagaaggtg ttacttacgc ccaaaaacta agcaaagaag aggcgcgtat
     7921 cgattggagc gaaagcgcgg cggttatcga acgcaaaatc cgcgccttca accccgtgcc
     7981 tgccgcgtgg gttgagtatc agggcaagcc gatgaaaatc cggcgggcgg aagtggtggc
     8041 gcaacaaggc gcggcaggcg aagtgttgtc ctgttcggcg gacggtttgg tcgttgcctg
     8101 cggcgaaaac gcgctgaaga ttaccgaatt gcagcctgcc ggcggcaggc gtatgaatat
     8161 cgcggcgttt gcggcgggac ggcatatcga agcagggacg aagctgtaaa tcccttcaga
     8221 cggcattccg atccgcaaac gggaatgccg tctgaaacca tcagttgaag aaagcgaatc
     8281 acataatatg agtatggcac ttgcccaaaa acttgccgcc gacagcattg cggcggttgc
     8341 cgaaggacgc aaccttcagg acgtgttggc gcaaatccgc accgcgcatc ccgaccttat
     8401 ggcgcaggaa aacggcgcgt tgcaggacat cgcctacggc tgccagcgtt atttgggcag
     8461 tttgaaacat atgctcgcgc agatgctgaa aaagccgatt ggcaatccgc agctcgaaag
     8521 cctgcttttg gcggcgttgt accagctgca ttacacgcgc aacgcgcccc acgccgtggt
     8581 caatgaggcg gtggaaagca tcgcgaaaat cggacgcggg cagtaccgtt cgtttgccaa
     8641 cgcggttttg cgccgctttt tgcgcgaacg cgacaagctt gtggcttcct gtaaaaaaga
     8701 cgatgtagcg aaacacaacc tgccgctgtg gtgggtggct tacttgaaaa accattatcc
     8761 gaaacactgg cacaacatcg ccgccgcgct gcaatcccat ccgccgatga cgttgcgcgt
     8821 caaccgccga cacggcaacg ccgaaagcta tttggaaaaa ctggctgcgg aaggtatcgc
     8881 cgcaaaggcg ttggacgaat atgcggttac gttggaagaa gccgtgccgg taaaccgtct
     8941 gcccggtttt tcagacggca ttgtttcggt acaggacttc ggcgcgcagc aggcggcgta
     9001 tctccttaac ccgaaagacg gcgaacggat tttggacgcg tgcgccgcgc cgggcggcaa
     9061 gacggggcat attttggaac tggcggattg ccgcgttacc gccttggaca tcgatgcagg
     9121 ccgtctgaag cgtgtggagg acaatatcgc gcgcctgggc tttcagacgg catcggcggc
     9181 gtgtgccgat gcgcgggatt tggcggcgtg gtatgatgga aagcctttcg acgcaatcct
     9241 tgccgacgtg ccatgcaccg cttccggcgt ggcgcggcgc aatcccgacg tgaaatggct
     9301 gcgccgtccg accgacgcgc tcaaaaccgc ccgccagcag gaagccctgc tggacgcact
     9361 ttggcagacg ctgaaacagg gcggcagaat gctgcttgcc acctgttccg tgttcgtcga
     9421 agaaaacgac gggcaattgc aaaaattcct caaccgccat gccgatgccg aaccgatcga
     9481 atcgcgggtg cttttaccga acaaacacca agatggcttt tattacgcgc ttattcaaaa
     9541 gcattaaaca atggcttgtg ctgctgccga tgctttccgt tttgccggac gcggcggcgg
     9601 aggggataga tgtgagccgc gccgaagcga ggataaccga cggcgggcag ctttccatca
     9661 gcagccgctt ccaaaccgag ctgcccgacc agctccaaca ggcgttgcgc cggggcgtgc
     9721 cgctcaactt taccttaagc tggcagcttt ccgccccgat aatcgcttct tatcggttta
     9781 aattggggca actgattggc gatgacgaca atattgacta caaactgagt ttccatccgc
     9841 tgaccaaccg ctaccgcgtt accgtcggcg cgttttcgac agactacgac accttggatg
     9901 cggcattgcg cgcgaccggc gcggttgcca actggaaagt cctgaacaaa ggcgcgctgt
     9961 ccggtgcgga agcaggggaa accaaggcgg aaatccgcct gacgctgtcc acttcaaaac
    10021 tgcccaagcc atttcaaatc aatgcattga cttctcaaaa ctggcatttg gattcgggtt
    10081 ggaaacctct aaacatcatc gggaacaaat aatgcgccgt tttctaccga tcgcagccat
    10141 atgcgccgtc gtcctgttgt acggactgac ggcggcaacc ggcagcacca gttcgctggc
    10201 ggattatttc tggtggattg ttgcgttcag cgcaatgctg ctgctggtgt tgtccgccgt
    10261 tttggcacgt tatgtcatat tgctgttgaa agacaggcgc gacggcgtat tcggttcgca
    10321 gattgccaaa cgcctttctg ggatgtttac gctggttgcc gtactgcccg gcgtgtttct
    10381 gttcggcgtt tccgcacagt ttatcaacgg cacgattaat tcgtggttcg gcaacgatac
    10441 ccacgaggcg cttgaacgca gcctcaattt gagcaagtcc gcattgaatt tggcggcaga
    10501 caacgccctc ggcaacgccg tccccgtgca gatagacctc atcggcgcgg cttccctgcc
    10561 cggggatatg ggcagggtgc tggaacatta cgccggcagc ggttttgccc agcttgccct
    10621 gtacaatgcc gcaagcggca aaatcgaaaa aagcatcaac ccgcacaagc tcgatcagcc
    10681 gtttccaggt aaggcgcgtt gggaaaaaat ccaacaggcg ggttcggtca gggatttgga
    10741 aagcataggc ggcgtattgt acgcgcaggg ctggctgtcg gcgggtacgc acaacgggcg
    10801 cgattacgcc ttgtttttcc gtcagccggt tcccaaaggc gtggcagagg atgccgtctt
    10861 aatcgaaaag gcaagggcga aatatgctga gttgagttac agcaaaaaag gtttgcagac
    10921 ctttttcctg gcaaccctgc tgattgcctc gctgctgtcg atttttcttg cactggtcat
    10981 ggcactgtat ttcgcccgcc gtttcgtcga acccgtccta tcgcttgccg agggggcgaa
    11041 ggcggtggcg caaggcgatt tcagccagac gcgccccgtg ttgcgcaacg acgagttcgg
    11101 acgcttgacc aagttgttca accacatgac cgagcagctt tccatcgcca aagaagcaga
    11161 cgagcgcaac aggaagcgcg aagaagccgc caggcattat cttgaatgcg tgctggaggg
    11221 gctgaccacg ggcgtggtgg tgtttgacga acaaggctgt ctgaaaacct tcaacaaagc
    11281 ggcggaacag attttgggga tgccgcttac ccccctgtgg ggcagcagcc ggcacggttg
    11341 gcacggcgtt tcggcgcagc agtccctgct tgccgaagtg tttgccgcca tcggcgcggc
    11401 ggcaggtacg gacaaaccgg tccatgtgaa atatgccgcg ccggacgatg ccaaaatcct
    11461 gctgggcaag gcaaccgtcc tgcccgaaga caacggcaac ggcgtggtaa tggtgattga
    11521 cgacatcacc gttttgatac acgcgcaaaa agaagccgcg tggggcgaag tggcaaaacg
    11581 gctggcacac gaaatccgca atccgctcac gcccatccag ctttccgccg aacggctggc
    11641 gtggaaattg ggcgggaagc tggatgagca ggatgcgcaa atcctgacgc gttcgaccga
    11701 caccatcgtc aaacaggtgg cggcattgaa ggaaatggtc gaagcattcc gcaattatgc
    11761 gcgttcccct tcgctcaaat tggaaaatca ggatttgaac gccttaatcg gcgatgtgtt
    11821 ggcattgtat gaagccggtc cgtgccggtt tgcggcggag cttgccggcg aaccgctgat
    11881 ggtggcggcg gatacgaccg ccatgcggca ggtgctgcac aatattttca aaaatgccgc
    11941 cgaagcggcg gaagaagccg atgtacccga agtcagggta aaatcggaag cggggcagga
    12001 cggtcggatt gtcctgacgg tttgcgacaa cggcaaaggg ttcggcaggg aaatgctgca
    12061 caacgccttc gagccgtatg taacggacaa accggcggga acgggattgg gtctgcctgt
    12121 ggtgaaaaaa atcattgaag aacacggtgg ccgcatcagc ctgagcaatc aggatgcggg
    12181 tggtgcgtgt gtcagaatca tcttgccaaa aacggtaaaa acttatgcgt agcagcgata
    12241 ttttaattgt agacgacgaa atcggcatcc gcgacctgct gtcggaaatc ctgcaggacg
    12301 aaggttattc ggtcgcattg gcggaaaacg ccgaagaggc gcgcaagctg cgccatcagg
    12361 cgcgccccgc gatggtgctg ctggatattt ggatgcctga ttgcgacggc atcacccttt
    12421 tgaaggagtg ggcgaaaaac gggcagctca atatgccggt ggtgatgatg agcgggcatg
    12481 ccagcatcga taccgccgtg gaagccacca aaatcggtgc gctcgacttt ttggaaaaac
    12541 cgatttccct gcaaaagctg ctgtccgcgg ttgaaaacgc gttgaagtac ggtgcggcgc
    12601 aaaccgaaac ggggcctgta ttcgacaagc tgggcaacag tgcggcgatt caggaaatga
    12661 accgtgaggt aggggctgcg gtgaaacgtg cctctcccgt acttttgacg ggcgaggcag
    12721 gttcgccgtt tgaaacggtg gcacgctatt tccataaaaa cggtacgccg tgggtcagcc
    12781 cggcaagggt cgaatatctg atcgatatgc cgatggaact gttgcagaag gcggagggcg
    12841 gcgttttgta tgtcggcgac atcgcccaat acagccgcaa catccaagcc ggcattgcct
    12901 ttattgtcgg caaggcggaa cgctgccgcg tcagggtggt cgcatcgggc agcagggcgg
    12961 caggttcaga cggcatagcc tgcgaggaaa agctggcgga actgctgtcg gagtcggtcg
    13021 tccgcattcc gccgctgcgt atgcagcatg aagatattcc cttcctgata caggggattg
    13081 cctgcaatgt ggcggaaagt caaaagattg cgcccgccgc attcagtgaa gaggcacttg
    13141 ccgcattgac ccgttacgac tggccgggaa atttcgacca actgcaaagc gtcgttgcaa
    13201 cgctgttgtt ggaggcggac ggacaggaaa tcggcgcggc ggcggtttct tcccttttgg
    13261 ggcagaatgt gcctgccgag ggagcggaag atatggtggg cgggtttaat ttcaacctgc
    13321 ccctgcgcga attgagggag gaggtggagc ggcgttattt cgagtaccac atcgcccaag
    13381 aaggtcagaa tatgagcaaa gtggcgcaga aagtcggttt ggaacgcacg cacctttacc
    13441 gcaaactcaa acagctcggc atcggcgttt cgcgccgggc gggggaaaaa accgaagaat
    13501 aggcccggac ggccggtttt ccggctgcgg gcttttgttt tcagacggca tttggtgcaa
    13561 atgccgtctg aaatcgtaag gggacggatt ttatgacaga ggacgaacgt ttcgcgtggc
    13621 tgcaattggc gtttacgccc tatatcggcg cggaaagctt cctgctgctg ctgcgccgtt
    13681 tcggcagcgc gtcaaacgcc ctgtccgcac cggcggaaca ggtcgccccg gcggtgcggc
    13741 acaagcaggc ggccgaggct tggcgcaatg cggaaaaacg cgctttggcg cggcaggcgg
    13801 cagaagcggc gttggaatgg gaaatgcggg acggatgccg cctgatgctg cttcaggatg
    13861 aagatttccc cgaaatgctg acgcaggggc tgaccgcgcc gccggttttg tttttgcgcg
    13921 gcaacgtgcg gctgctgcac aagccttccg ccgccatcgt cggcagccgc catgccacgc
    13981 cgcaggcgat gcggattgcc aaagatttcg gcaagtcgtt gggcgggcaa aatatccccg
    14041 ttgtgtcggg tatggcttcc ggtatcgata ctgccgccca tcagggcgca ctggaggcag
    14101 aaggcggcac catcgccgtg tgggggacgg gtatagaccg catttacccg ccgtccaata
    14161 aaaaccttgc ctatgaaatc gccgaaaaag gattgattgt cagcgagttt cccatcggta
    14221 cgcgcccgta tgccggcaat tttccgcgcc gcaaccgcct gattgccgcc ctgtcgcaag
    14281 taacgctggt ggttgaagcc gccttggaat ccggttcgct gattaccgcc ggcttggcgg
    14341 cagaaatggg gcgcgaagtg atggcggtac ccggttcgat agacaatccg cacagcaaag
    14401 gctgccacaa actgattaaa gacggcgcga aactgacgga atgcctggac gacatcctga
    14461 acgaatgccc ggggctattg caaaatacgg gtgcttcatc atattctata aataaggata
    14521 cgcccgacac gggcagtcgc gccgttcaga cggcatacgc cccgccgcct gccgcaaaaa
    14581 tgccgtctga aggggcggca ggcggcacag ccgtcggcgg catactggat aaaatgggtt
    14641 tcgaccccat ccatcccgac gtgcttgccg gacagttggc tatgcctgcc gcagatttgt
    14701 atgccgcact gttggaattg gaattggacg gcagcgttgc cgcaatgccc ggcggcagat
    14761 accagcgtat ccgaacttga acgcacttta tattaaggaa cacgaatgac cgaagtcatc
    14821 gcctacctca tcgaacattt ccaagatttc gatacctgcc cgccgcccga agacttgggt
    14881 atgctgcttg aagaagcggg tttcgatacg atggaaatcg gcaacaccct gatgatgatg
    14941 gaagtattgc tcaacagctc cgaattttcc gccgaacccg ccgacagcgg cgcattgcgc
    15001 gtgtacagca aagaagaaac cgataacctg ccgcaggaag tgatggggct gatgcagtat
    15061 ctgattgaag aaaaagccgt cagctgcgaa cagcgggaaa tcatcatcca cgcgctgatg
    15121 cacattccgg gcgacgaaat taccgtagat accgccaaag tgctgaccct gctgctttta
    15181 tgggcaaaca agagcgagct gcccgtgttg gtcggcgacg agctgatgag cgcgctttta
    15241 ctcgacaaca aacccacgat gaactgaagc ggcttcagac ggcccgcccg agtccgtctg
    15301 aaacgtcggc atcaaaacca ccatccagag aacgacaaat ggcgaaaaac ctcttaatcg
    15361 tcgaatcccc gtccaaagcc aaaaccctga aaaaatattt gggcggcgat tttgaaatcc
    15421 ttgcatccta cggacacgtc cgcgacctcg tccccaaaag cggcgcggtc gatcccgaca
    15481 acggctttgc gatgaaatac caactcatca gccgcaacgg caaacacgtc gatgccatcg
    15541 tcgccggtgc caaagaagct gaaaacatct acctcgccac cgacccggat agggaaggcg
    15601 aagccatttc ctggcatctt ttggaaatcc tcaaatccaa acgcggcttg aaaaacatca
    15661 agccgcagcg tgtcgtgttc cacgaaatca ccaaaaacgc cgtgctcgat gccgttgccc
    15721 atccgcgcga aatcgaaatg gacttggtcg atgcgcaaca agcccgtcgc gctttggact
    15781 atttggtcgg tttcaacctt tcgccattgt tgtggaaaaa aatccgtcgc ggtttgagcg
    15841 cgggccgtgt acaaagccct gctttgcgcc tgatttgtga gcgcgaaaac gaaatccgcg
    15901 cgtttgaagc gcaggaatat tggacggtgc atctcgacag ccacaaaggc cgcagcaagt
    15961 tcaccgccaa actcgcccaa tacaacggcg cgaaactcga acaattcgac ctgtcgaacg
    16021 aagccgcgca agccgacgta ttgaaagaac tcgaaggcaa agaggccgtc gttaccgcca
    16081 ttgaaaagaa aaagcgcagc cgcaatcccg ccgcgccgtt caccacttct accatgcagc
    16141 aggatgctgt gcgcaaactc ggcttcacca ccgaccgcac tatgcgtacc gcccagcagc
    16201 tttacgaggg gatagacgta gggcagggcg ccatcggtct gattacctat atgcgtaccg
    16261 acagcgtgaa tttggcagat gaagcgttaa ccgaaatccg ccattacatc gaaaacaaaa
    16321 tcggcaaaga atatctgccg agtgccgcca aacaatacaa aaccaaatcc aaaaacgccc
    16381 aagaagcgca cgaagccatc cgtccgactt ccgtgtaccg cacgcccgaa agcgtcaaac
    16441 ccttcctgag cgcagatcag ttcaaactct atcagatgat ttggcaacgc accgttgcct
    16501 gtcaaatgac gcctgccaaa ttcgaccaaa ccaccgtcga tattaccgtc ggcaaaggcg
    16561 tattccgcgt aaccggacaa gtgcaaacct tcgcaggctt cttgagcgtt tatgaagaaa
    16621 gcagcgacga cgaagaaggt gaagacagca aaaaactgcc cgaaatgagc gaaggcgaca
    16681 aattgcccgt ggacaaactc tacggcgaac aacactttac cactccgccg ccacgctaca
    16741 acgaagccac gctggttaaa gccctcgaag aatacggcat cggccgcccc tcgacctacg
    16801 ccagcatcat ctccacgctc aaagaccgcg aatacgttac ccttgagcaa aaacgcttta
    16861 tgcccaccga cacaggcgac atcgtcaaca aattcctgac cgaacacttc gcccaatacg
    16921 tcgattacca ctttaccgcc aaactcgaag accagcttga cgaaatcgcc ggcggcaaac
    16981 gccaatggat tcctgtgatg gacaaattct ggaagccgtt catcaagcaa gtggaagaaa
    17041 aagagggcat cgaacgcgcc aaatttacca cgcaggaact tgacgaaacc tgtccgaaat
    17101 gcggcgaaca caaactgcaa atcaagttcg gcaaaatggg ccgtttcgtc gcgtgtgccg
    17161 gttatcccga gtgtagctac acgcgcaacg tcaacgaaac cgccgaagaa gccgccgagc
    17221 gcatcgccaa agccgaagcc gagcaagcag aactcgacgg acgcgagtgc cccaaatgcg
    17281 gcggacggct ggtgtacaaa tacagccgta ccggcagtaa attcatcggt tgcgccaact
    17341 accccaaatg caaacacgtc gagccgctgg aaaaaccgaa agataccggc gtccagtgtc
    17401 cgcaatgtaa aaaaggcaac ctcgtcgagc gtaaatcccg ctacggcaaa ctgttttaca
    17461 gttgcagcac ctatcccgac tgtaactacg ccacctggaa cccgcccgtt gccgaagaat
    17521 gcccgaactg ccattggccg gtcttgacca tcaaaaccac caaacgctgg ggtgtggaaa
    17581 aagtctgccc acaaaaagaa tgcggctgga aagaacagat tgaaccgccc gcgccgaagg
    17641 agtaagatta ggttggtttg aaagagaaaa ggtcgtctga aaaattttca gacgaccttt
    17701 gcttttctgt gattggttta tttgaatccg cgtgttgttt taaagtccga taaaatccgg
    17761 ttcatttcag gcgcaaacaa ggcgatgtaa tcgtaagata gaccgcgact ggcactggga
    17821 tggggaaagc agacgacttc gcaatcttca aacgattgga atttgacatt gaaacgtgta
    17881 ccgtcaaatt ctttttgcac cgtctccagc ggtttggtct gcttaccgac caactgctcg
    17941 aagcgtggca gtacattttg gttgttcaga aaatccgcca acctgctgcc catgaagagg
    18001 atgactttcg gacgcagttt ttcgatgtgg tagagaaaat tatcgatgtg ctcgggttgt
    18061 gtgaacttgt cgggattgtc gatagtgttg ccctgtgtag cagcccagtt ggtttgaacc
    18121 agggattttt caaatgcacc gcccaatcca ttttcgtcta aggggtgtcc ccacatttca
    18181 aaccaatttt ttatcgtatt gtcgtaacgc cacttctttg cctgctctcc gaaatagagg
    18241 gatttgtttg caaatgtatg gtcgattttg ttttcaggga gtttgtattc acctgctaca
    18301 taagcagcct catcggcttt actccaaccc cattcatacc cacaaatcat taaaccatgt
    18361 ttgtcgttgt agcctttgaa cagactgttg ctcaaattca aatccttcat catgaactct
    18421 tccttttaaa atttaagagc gattgacttc aatgttttta gatggggtgg aaaaatcctt
    18481 gtgtaggcaa cataaattca ataaatttct tgataattcg aaacctacta atagcgcacc
    18541 tataaaagct ttttcattac gttcagcatg acggtcacgt cgttcatatt ttttacgctt
    18601 gctgttccct gttattacag ctaagccaag tgatatggcg agaattgccc aaacaattgt
    18661 acttaataac aattttcccc atactatcaa taaggaaaga aaaaaccttt tagtattaga
    18721 tcgataggtt ataatccatg cccatgaaaa tgcaataaga gttatacata agatacaagc
    18781 tgataggatt tctttatttt taattaaata acttagggcg atgaggatga caggtgtcaa
    18841 gaaaacaata gttacatccc gatagctata aaagaaaact gccctatttt gaatgtggag
    18901 atgtgcacag aatccaatat agctaaggat aatagttaat ataataaaaa aagaccacca
    18961 agggtgaaga gataggaatt ccatgttttc cctttttttg taaaaaggaa aaaatcttat
    19021 ctaagagtta attgcctaac cagccagaag aagtttaaaa tctatcccaa taattcaacc
    19081 atctatacag aaagttcagc ttatggaaac ccacgaaaaa atccgcctga tgcgcgaatt
    19141 gaataaatgg tcccaggagg atatggcgga aaagctggcg atgtcggcag gcgggtatgc
    19201 caaaatcgaa cggggcgaaa cgcagttaaa tatcccgcgt ttggagcagt tggctcagat
    19261 tttcaaaatc gatatgtggg acttgctcaa atcgggcggt ggtgggatgg tgtttcagat
    19321 taatgaaggt gatagtggtg gcgatattgc gttgtatgcg tcgggtgatg tttcgatgaa
    19381 aatagaattt ttaaaaatgg agttgaaaca ctgcaaagaa atgttggaac aaaaagacaa
    19441 agaaatcgag ctgctccgca agctgaccga aaccgtttaa acagatatgc cgtctgaaaa
    19501 aag
//
LOCUS       DAXHRI010000024        18380 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXHRI010000024 DAXHRI010000000
VERSION     DAXHRI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.024"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            716..2164
                     /gene="gnd"
                     /locus_tag="ACFM75_000642"
     CDS             716..2164
                     /gene="gnd"
                     /locus_tag="ACFM75_000642"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC6255228.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2227..3498
                     /gene="waaA"
                     /locus_tag="ACFM75_000643"
     CDS             2227..3498
                     /gene="waaA"
                     /locus_tag="ACFM75_000643"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC6255229.1"
                     /translation="MFQWLYDALWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3539..3988
                     /locus_tag="ACFM75_000644"
     CDS             3539..3988
                     /locus_tag="ACFM75_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC6255230.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4021..4995
                     /locus_tag="ACFM75_000645"
     CDS             4021..4995
                     /locus_tag="ACFM75_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC6255231.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFVFLKYGLA
                     FVLGFIGVKMLIMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            5064..5139
                     /locus_tag="ACFM75_000646"
     tRNA            5064..5139
                     /locus_tag="ACFM75_000646"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5097..5099,aa:Lys,seq:ttt)
     gene            5358..6611
                     /gene="murA"
                     /locus_tag="ACFM75_000647"
     CDS             5358..6611
                     /gene="murA"
                     /locus_tag="ACFM75_000647"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC6255232.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6692..7870
                     /locus_tag="ACFM75_000648"
     CDS             6692..7870
                     /locus_tag="ACFM75_000648"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC6255233.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASIASV
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGSLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALTEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7937..8185)
                     /locus_tag="ACFM75_000649"
     CDS             complement(7937..8185)
                     /locus_tag="ACFM75_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6255234.1"
                     /translation="MLTPEQVKAMIEDVAKCEHIKVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8275..9192)
                     /gene="ftsX"
                     /locus_tag="ACFM75_000650"
     CDS             complement(8275..9192)
                     /gene="ftsX"
                     /locus_tag="ACFM75_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC6255235.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPEEIV
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9189..9839)
                     /gene="ftsE"
                     /locus_tag="ACFM75_000651"
     CDS             complement(9189..9839)
                     /gene="ftsE"
                     /locus_tag="ACFM75_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC6255236.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPSLLI
                     ADEPSANLDRAYALDIMELFKTFHESGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10034..10516)
                     /locus_tag="ACFM75_000652"
     CDS             complement(10034..10516)
                     /locus_tag="ACFM75_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC6255237.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10640..10993
                     /gene="arsC"
                     /locus_tag="ACFM75_000653"
     CDS             10640..10993
                     /gene="arsC"
                     /locus_tag="ACFM75_000653"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC6255238.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10990..11646
                     /locus_tag="ACFM75_000654"
     CDS             10990..11646
                     /locus_tag="ACFM75_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC6255239.1"
                     /translation="MKKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKHPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGWDFFNWLFH
                     "
     gene            11838..13232
                     /gene="gltX"
                     /locus_tag="ACFM75_000655"
     CDS             11838..13232
                     /gene="gltX"
                     /locus_tag="ACFM75_000655"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC6255240.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEEGKTLPEIPADVQPVVRFKTPLDGVTQWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNDKLAELVKPRLA
                     LRDIHETEKPALEDVLAQVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEASAR
                     MLRFAERLEGLEDWNTEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13253..13648
                     /locus_tag="ACFM75_000656"
     CDS             13253..13648
                     /locus_tag="ACFM75_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255241.1"
                     /translation="MPSDVGIRLQTAFKRKLNMKKILYFLIFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13652..14143
                     /locus_tag="ACFM75_000657"
     CDS             13652..14143
                     /locus_tag="ACFM75_000657"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6255242.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSETLLGSAAVKRHDMESFPRYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14267..16861)
                     /gene="mutS"
                     /locus_tag="ACFM75_000658"
     CDS             complement(14267..16861)
                     /gene="mutS"
                     /locus_tag="ACFM75_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC6255243.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHTAALNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELEDQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17303..18307
                     /gene="gap"
                     /locus_tag="ACFM75_000659"
     CDS             17303..18307
                     /gene="gap"
                     /locus_tag="ACFM75_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6255244.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQNILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
BASE COUNT         4200 a         4485 c         5298 g         4397 t
ORIGIN      
        1 agcggtttct gttttttctg ataaattcct aaaacttaaa atttcatcat tcccactttc
       61 gtgggaatga cgggatgtag gttcgtggga atgacgcggt acaggtttcc gttacgtatc
      121 cccatccgaa agtttattta tacacaagca atccaaagtt atctgactga aatattgtga
      181 gatttacccg caaatctcat tttttccacc acttatacac aaattcggat gcgtactctg
      241 ccgatatgca gaaataagac attcgtgccg acacctgacc atttccacaa gttccaaaca
      301 cgcttccggc ttaaatctcc acagtattcc cgttcttaaa ggcgccagca gccattcggt
      361 aaagaaaagt aggatgccat ctgcacaaca agcaaactat ccacaaaact tattccccat
      421 aaaaaacaat ccacaggtat gcacaaaagg gaatccgcct taccgacacc cttttaaaac
      481 ggaaatccca atgattttta aaataaaaaa aagttaccca cagaaaattt ctcctatcaa
      541 tataatcatc gtttttttat ttaattttta tattttattt ataagccttc agggaaacgc
      601 acggtaaagc gtaaaggcat cttttcaaaa aattaaaaag ttaaggattg ttaaacaagt
      661 gtaaatcagg tagagttgcg gtcgataaat taaatctata acggagcttc ctgcaatgaa
      721 aggcgatatt ggcgtaatcg gtttggcggt tatggggcaa aacctgattt tgaatatgaa
      781 cgattgcgga tttaaggttg tcgcctacaa ccgaacaatc ggtaaggtgg acgaattttt
      841 aaacggcgcg gctaagggaa cggatattgt cggcgcgtat tccctgcaag acttggtcga
      901 taagctggca aaaccgagaa aaatcatgat gatggttcgc gcaggttcgg tggttgacga
      961 ctttatcgaa caactgctgc ccctgttgga cgaggaagac atcattatcg acggcggcaa
     1021 tgccaattac cccgacacga cacggcggac acattacctt gccgaaaaag ggattttgtt
     1081 tgtcggggcg ggcgtatccg gcggcgaaga aggcgcgcgg cacggcccgt ccattatgcc
     1141 gggcggggat aagcgcgctt gggaagcggt caaaccgatt tttcaggcga ttgccgccaa
     1201 aacgccgcag ggcgaaccgt gttgcgattg ggtcggcagg gatggtgcgg gacatttcgt
     1261 caaaatggtg cataacggca tcgaatacgg cgatatgcag ttgatttgcg aagcgtacca
     1321 gttcatgaaa gacggtttgg gactgtccta cgacgaaatg caccgcgtgt ttgccgagtg
     1381 gaacaaaacc gagctggatt cttatctgat tgaaatcaca gcggcaattt tgggctataa
     1441 ggacgaaggc ggcgaaccgc tggtcgagaa aatcctcgat acggcagggc aaaaaggcac
     1501 gggcaaatgg acgggcatca atgctttgga tttgggtatt ccgttgacgc tgatttccga
     1561 agcggtattc gcccgctgcg tttcgtcgtt caaagaacag cgcgtgcaga ccggcaagct
     1621 gttcgcccga accgttacgc ccgtcgaagg cggcaaacaa gaatgggttg aggcgttgag
     1681 acaggccctg cttgcatcca aaatcatttc ctacgcacaa ggctttatgc tgatccgcga
     1741 agcgggcgaa agctacggct gggatttgga ctacggcaac actgcgctgc tgtggcgcga
     1801 ggggtgcatc attcgcagcg catttttaag caatatccgc gatgcctatg aaaacaatcc
     1861 cgatttggtg ttcttgggtg cggatccgta tttcaaaaat attttggaaa actgcctgcc
     1921 ggcgtggcgc aaggtggttg ccaaggcggt cgaatgcggc atccctatgc cctgcatggc
     1981 ttcggcgatt acgttcttgg acggctacac gaccgaacgt ctgcctgcca acctcttgca
     2041 ggcacagcgc gactacttcg gcgcgcacac ttacgaacgc accgacaaac cgcgcggcga
     2101 gtttttccat accaactgga cgggcaaggg cggagatacc gcttcgacaa cctacgatat
     2161 ttgattccgt tgccgaaatg ccgtccgaac cgttcggacg gcatttgttt tgcaggaaat
     2221 agaaagatgt tccaatggct ttatgatgca ttgtggctgc ttgcgccgat atggatacgg
     2281 cgttatttgg acaaacgctc cggaagtgcc cctgcatatc gggcgcatcg ggacgagcgt
     2341 ttcggcaagc cgtatccgaa tcccgttacc ggcgcggttt ggatacacgc cgtttcggtc
     2401 ggagaaacgc gtgccgccca gcccttgata cgcgagttgc ggcagcgttt tcccgatgcg
     2461 ccgctgctga tgacgcagat gaccccgacg ggacgggaaa ccgcgcaagt tctgtttccc
     2521 gatgcgcaat gccgctatct tccgtatgac aaaaaaacgt gggtacggca gtttttgcgc
     2581 gaacaccgcc cgatgttcgg cattttgatg gaaaccgaaa tctggcccaa tctgatgaag
     2641 gaatgccggc gcgcgggtgt gccgctgttt ttggcgaatg cgcggctgtc ggaaaaatcg
     2701 ctgaacggtt atctgaaagt ccgccgcctg atccgtcctg ccgccgcttc gctgacgggc
     2761 tgtctggcac agacggaggc ggatgcggcg cggttggcga aattgggcgc ggcatccgtg
     2821 caggtgtgcg gcaataccaa atacgacctg atgccgtcgg aacagatgaa aacgctggcg
     2881 gggcagtttg aaaaacgcat cggaggccgg ccggttgccg tgtgcggcag cacgcgcgtt
     2941 tatcggggcg aagacgaggc ggaaaaactg ctggcggcgt ggcaacaata tcgcggcgat
     3001 gcgctgctgg tcgtcgtgcc gcgccatccc gagcattttc agacggtatt tgaaacggca
     3061 aaacgcttcg ggtttaaggt tcagcggcgc agcgacggtt tgccggtcga acctgatacg
     3121 caggtgtgga taggcgacag tatgggcgag ctgtatgcgt attacctgtg cgccgatgtc
     3181 gcttttgtcg gcggcagtct ggtcgattcg ggctgtcaga acattatcga accgctttcc
     3241 tgcggcgttc cgacgatatt cggcttttca acctacaatt tttccgaagc ctgccggcac
     3301 gccttggcat cgggggcggc ggttcgggtt gagtcggcgg acgcgtggcg ggaagccgtt
     3361 gaaaaaacct tatcgtccga ggggggcgga atgcagatgc aggcgcacgt ggacggcttt
     3421 atcgcacaac atcgcggagc gggcgcgaga atcgccgagg cggtacggga agcggtatgc
     3481 ggacatcggg gacgatagtg atacaatccg tatccgagtt ttccggttgg agcatcgtat
     3541 gagtatttat gccgtcgcgc acatcgtcca cctgtattgc gccattgcct ttgtcggcgg
     3601 cgtgtttttt gaagtgctgg ttttgtccgt cctgcatacg ggacgggtgt cgtgcgaggc
     3661 gcggcgcgaa gtggaaaagg caatgtctta ccgcgccgtc agggtgatgc cgtttgtggt
     3721 cggactgctg ttcgccagcg gcgtcgtgat ggcggcaaac cgctatcttt ctatattggg
     3781 cgaaccgttt gccacttcct tcggtacgat gctgacgctg aaaatcctgt tggcgttcag
     3841 cgtgttggcg cacttcgcca tcgccgtcgt caaaatggcg cgttccacac tgaccgtcgg
     3901 ctggtcgaaa tacatacacg ccgtcgtctt tacccatatg ctgctgattg tctttttggc
     3961 aaaagcgatg ttttatatca gctggtaaca caattacatt tcaaatcagg agattgaacc
     4021 atgacagaat atcccggcat cgggtcgccg ttgttttacg gcgttttttt tgcggcagtg
     4081 ctggtcatga ttgccttgga tatgttttcg ctgaagaaaa acggcagcca taaagtcggc
     4141 gtcaaagaag ccttggcatg gagcggcttg tgggttgccg tatcctgcct gttcgcgggc
     4201 tggctgtatt tcgaactcgc cggcaatctc ggctacggcg cggcagtcgc aaaagaaaaa
     4261 gtattggaat tctttaccgg ctacattttg gaaaaatcgt tggcagtcga caatattttc
     4321 gtgttcctga tgatattcgg ctacttcaaa gtcgcaccgc agtttcagca ccgcgtgctg
     4381 ctgtacggcg tattgggcgc attggtattg cgcactgtca tgattttcgt cggcgcggca
     4441 ctggttcagc agttcgagtg gattctgtat ctgttcggcg cgttcctgct ctataccggc
     4501 atacacatga tgaagcccga aggcgatgag gaaggggatt tggcaaacag caggctgctg
     4561 aatgccgtca agaaagtcgt tccggtcggc acggaatttc acggcgagaa attttttact
     4621 gtcgaaaacg gtaaaaaaat cgccacgccg ctgtttttgg tgctggtcat gattgaattg
     4681 agcgatgtcg tgtttgccgt ggacagcatc cccgccgtct ttgccgttac caccgatccg
     4741 tttatcgtgc tgacttccaa tattttcgca attctgggct tgcgggcgat gtatttcctg
     4801 ctggcggatg tggcggaacg cttcgtattc ctgaaatacg gcttggcatt cgtgttgggt
     4861 tttatcggtg tgaaaatgct gataatgcat tgggtgcata tcccgatttc cgtttcgctg
     4921 tcggtcgtgt tcggcgcatt gggtgcatcg gtgttgactt cattggttta tacgaaaaaa
     4981 cagtccgata aataaaatgc cgtctcaacg ccgacaaaat ggttgacagc atcaggcttg
     5041 atgtatagaa tttcgagctt gtcgggtcgt tagctcagtc ggtagagcag cggactttta
     5101 atccgttggt cgagcgttcg aatcgctcac gacccaccag ataaagcaaa aaagctgagt
     5161 tttcaaaact cggctttttt tgttttccga caatccttgt tcggaaagcg cggcatttcg
     5221 tcaaattctt tggtgcggct tccgtatttt tcccgaaaat cccttataat tccgcctctg
     5281 caaccttcat cgggtgatgc tttatgcgtt gtcccgagta ttgaaatatt acaagtaccc
     5341 accaggaaag aacaaacgtg gacaaactga aaatctccgc aaacggcccg ctcaacgggg
     5401 aaataacggt ctcgggcgcg aaaaacgcgg cattgccgct gatgtgcgcg ggtttgctga
     5461 catcgggtac gttgcgcctg aaaaacgtcc ctatgctggc agatgtgaaa accacgcaaa
     5521 agctgcttca ggggatgggc gcgcgcgtcc tgactgacaa tatcagcgaa tttgaaatca
     5581 acggcggtac ggtaaacaat acctgcgccc cttacgaatt ggtacggacg atgcgggcct
     5641 cgattttggt gctggggcct actttggcac gtttcggcga ggcgcaagtc agcctgccgg
     5701 gcggctgcgc catcggttcg cgccccgtcg atcagcattt gaaaggcttg gaagcgatgg
     5761 gtgctgagat tgttatcgaa cacggttacg tcaaagccaa aggcaaactc aaaggtacgc
     5821 gcgtggcgat ggatgtcgtt accgtcggcg gcacggaaaa cctgctgatg gcggcgacgc
     5881 tggcggaagg tacgacggtt ttggaaaact gcgccattga gcctgaagtg gtcgatttgg
     5941 cggaatgcct ggtcaaaatg ggcgcgaaaa tcagcggcat cggtacgtcc acaatgattg
     6001 tggaaggtgt ggacgagctg cacggctgcg aacacagcgt cgtccccgac cggattgaag
     6061 cggggacgtt cctgtgtgcg gtggcgataa ccggcggcag ggtggttttg cggaatgctg
     6121 cgccgaaaac gatggaagtg gtgttggaca aactggttga ggcaggtgcg gtgattgagg
     6181 cgggcgatga ttggatcgcc atcgatatgc ggcagcgtcc gaaggcggtg gacatccgca
     6241 cggtcgtcca ccccggcttc cccacggata tgcaggcgca gtttatggca ttgaatgccg
     6301 tggcggaggg aagctgccgc gtggtggaaa cgatttttga aaaccgcttt atgcacgtcc
     6361 ccgagttgaa ccggatgggg gcgaacatca caaccgaggg caatacggca tttgtgcagg
     6421 gtgtggaaca gctttccggc gcggtcgtca aggcgacgga tttgcgtgcg tccgcaagcc
     6481 tcgttatcgc cggtttggcg gcgcgcggcg aaaccgtggt cgaacagatt taccacttgg
     6541 atcgcggtta tgaaaatatt gaaaaaaaac tcggcagcgt cggcgcgaaa atcgagcgcg
     6601 tatccggctg atcgggttgc gatgccgtct gaagccttca gacggcatca attgtttccg
     6661 tatcgcaaac actgattgaa aaggactgat tatggcattt ttaaaactga ccgaacaaaa
     6721 cgtgcagggc aaaaccgtcc tcatccgcgc cgatatgaac gtgccgttca aagacggcaa
     6781 aatcagcgac gacacccgta tccgcgcctc gattgcgtcc gttaaatact gcgtggacaa
     6841 cggcgcgtcc gtcatcgtga tgacccactt gggccgcccg accgaaggcg agtttcaccc
     6901 cgaagacgat gtcgcgcccg ttgcagcaca cttgggcagc ctgttgggca aagacgtgaa
     6961 agtattgaac gactggcgcg aaaacaaacc ggctttgaac gcgggcgatg tcgtcatgct
     7021 gcaaaacgtg cgtatcaaca aaggcgagaa gaaaaacgat ttggaactgg gcaaagccta
     7081 cgccgccttg tgcgacgtgt tcgtcaatga cgcgttcggc accgcgcacc gcgcccaagc
     7141 ctcgaccgaa gccgtcgccc aagccgcgcc cgttgcctgc gccggcgtat tgatggcggg
     7201 cgaactcgac gctttgggca aagccttgaa acagcccgcg cgcccgatgg ttgccatcgt
     7261 tgccggcagc aaagtgtcca ccaagctgac catcctcgaa tcgctggcgg acaaagtcga
     7321 ccaactcatc gtcggcggcg gtatcgccaa caccttcctg ttggcggaag gtaaagccat
     7381 cggcaaatct ttggcggaac acgatttggt ggaagaatcc aaaaaaatta tggcgaaaat
     7441 ggcggccaaa ggaggttctg tgccgctgcc gaccgatgtc gtcgttgcca aagcctttgc
     7501 cgccgatgcc gaagcggtgg taaaagacat tgccgacgtt gccgaagacg agatgatttt
     7561 ggacatcggc ccgaaatctg ccgccgcgct tgccgatttg cttaaagccg ccgacacggt
     7621 cgtttggaac ggaccggtcg gcgtgttcga gtttgaccag ttcgcaggcg gtacgaaagc
     7681 ccttaccgaa gccattgccc aaagcaaagc gttctccatt gctggcggcg gcgacacgct
     7741 ggcggcgatt gctaaattcg gcgttaccga ccaaatcggc tatatttcta ccggcggagg
     7801 cgcgtttctt gagttcttgg aaggtaaaga gttgcctgcc gtagccgctt tggaaaaacg
     7861 cggcgcgtaa gcggtttgat ataaaaaatg ccgtctgaaa cgttgtgctg tttctgacgg
     7921 catttttgtg tggcgattat tgtgctttgg ctgcccattc cgccggagtg gcggcaaccg
     7981 aaatggaaag tgcgtgcagt tcgttacttt ccagttgggc tttgagtccg tctttaatca
     8041 ggcggtggcg cgcgaggcgt gccttgcctt caaattctga tgaaacgatg acggcgaaaa
     8101 aatggtgtcc gtcgccttct actttgatat gttcgcattt tgccacgtct tcaatcatgg
     8161 cttttacttg ttccggggtc agcatggtag gttccttgtt ggaaaaatgc gccattatac
     8221 tggcaaattg aaatgccgtc tgaaacgggt tcagacggca tttttgacgg tgttttattt
     8281 tttggctttg aagccgagca ggtgttgggt ggtggcaagc cacgcgccga atacgcccaa
     8341 cgcgatgacg aagccgaaca ccagcacgat ttcttcaggg taaaagaaac gccagccgat
     8401 gttcaagccg tagggtttga aaatggcatc gaccaatggg cgtacggcag agagcagcca
     8461 accgcaaagc cccaagctga cggcggcgga aaggatgctc tgccacatgg cttgataaag
     8521 gaatgggcgg cggataaacg acgcgggcgc gcccaagagt ttggtgattt cgatttcttc
     8581 ttttcggctg aggatttgca gtcggatggt gttgtgcgcg acaaggacga acgccatccc
     8641 cagcgtcagt gaaagaaacc ataaaatttt gcggatgaat tcgttgattt ggtacagcgt
     8701 ttgcacccat tcggtatcca tagacgcgga ttcgaccata ggcagtttgg taatgtctcg
     8761 gtagattgcc tgcatttggg cgggcgtggt tgccgggtcg ggggtaacga taaagacatc
     8821 cggcaggggg ttgccgtcaa gcatggaaat cagattttgg tcgagattgg actgtaattc
     8881 ttccagaccg tcttctttgc cgatgaagcg gatgttgtcg agccgtttgt cgcgcgccag
     8941 caggctgcgc acggtatcgc tgtcgctttg tgcggcggcg gtttccatat agattgtgat
     9001 ttgcggcgac tcgttgagtt tgcccaacac gctttgcccg ctttggatgc ccaaatacat
     9061 aaacagcggc agggtcatgg cgacggcgag cattatgagg gtaagcagtg tgccgaaagg
     9121 ttggcgcaga agctgtttga gcgcggtgcg cgcggattcg acgtgcagcg agaggtagtg
     9181 gatgatgctc atgcgagtcg tcctttcgag aggcgcagga tgcggtgtcc gtagtccgcc
     9241 atcagggttt cgtcatgtgc ggcgacgatg acggttgttc ccgattcgtg gaaggttttg
     9301 aacaattcca taatatcgag cgcgtaggcg cggtcgaggt tggcggaggg ttcgtcggca
     9361 atcagcaggc tgggctggtg aacgacggcg cgggcgatgc acaggcgttg ttgttcaccg
     9421 ccggagaggg ttacgggatc gtccaattct cgtcctttca ggccgacttt ttcgatggcg
     9481 atgcgggcac gctcttcggc tttgcgcggc ggatagccga taatccgaag cggcaggatg
     9541 acgttttgca ggacgttgcg gtcgtagagg attttgtggt cttggaacac gatgccgatg
     9601 tgttggcgca taaagccgat ttggttgtcg gacaatgtgc cgagatcctg cccgttaaac
     9661 aggattttgc ccctgctcgg cttggtgatg cccgaaatca gcttgaggac ggtggatttg
     9721 cccgaaccgg agtgtcccgc gataaatatc atttcgcctt tgttgatttg gaagctgacg
     9781 tttttcaggg cttcaaaacc gccgggatag gttttggaaa cttgttcgaa acggatcata
     9841 ggaggtcctg taaggaaggg cggcatatcg tttcgggcgg ttgcgtccgc agggatatgc
     9901 cgtctgaaca ataggtaaga cagtgccgat tataaagatt ccgcctgttt ttagatagtg
     9961 gataagtttg gcggataccg aaggtcgggc gggaaaaatg ccgtctgaag cggattcaga
    10021 cggcatccgg cgtttaacgg cattttgaat gggcgagttt gacggcttcg gtcaggcttt
    10081 tttcgttcac ttccccggta atggtctgcc tgtatccgca tttcggtgct tcgacgacgg
    10141 taaagggcag tacgccgaca gtgtttccgt aggttttcat aaagtttcgg ctgttcgccc
    10201 cggtgtaacg ccaaatcggg taggaaacag gagtttgttt gaggaagttg ccgatattgt
    10261 cggatgtgtc gagcgcgatg ccgaccatat cgacgctgcc ttttttctgc gctttgtacc
    10321 atttggacat ggcaggcatc tcttttcggc acggaccgca ccaggttgcc caaaggttga
    10381 cgatgcgtac gggggctttg agtgattgca ggctttgcgg ggtgttgtct ttccatccgg
    10441 ccagttcgtc tgccgaggcg gtatgtgcgg caaatgtggc ggcaagggtg attgcggcaa
    10501 gattcaagta tttcatcgtt ttgtcctttg tatgtcgatt cgggtattgt aaaccgtttg
    10561 gtacggttgt aggcgggata ttgagtaaaa ccctgtgtaa gccgataata aggggattgt
    10621 cgagaagtaa ggaaatgtca tgtctgaaat caaaattttc cacaatccgc gttgcagcaa
    10681 atcgcgtgcc gccttgtccc tattggaaga acgcggcatt gctgccgaag tggtcaaata
    10741 tttggatacg ccgcccgact tgtccgaatt gaaggatatt ttcaacaaat tgggtttggc
    10801 atcggcgcgc gggatgatgc gcgtgaaaga tgatttgtac aaggaattgg gtttggacaa
    10861 ccccgatttg gacaatgacg cgctgctgcg tgccatcgcc gatcatcccg ccctgttgga
    10921 gcgtccgatt gttttggcga acggtaaggc ggctgtaggc aggccgctgg aaaatatcga
    10981 agcggtattg tgaaaaaacg gctgttttgc agccgcaacg gcttgcgcta ttacctgttg
    11041 ggcggtttct gtttgagtgt ttttccgctt ttgctggtgt ttgcctcatc cgtttgggcg
    11101 gtttatcgga cgggcggtca ggttttgccg ccgtatgtcc gggcggatgc cgcgctggtg
    11161 ttgggcgccg ccgcgtggga caaacaccct tctccggttt ttcgggaacg catcaaccac
    11221 gccatcgcgc tttatcagag ccgcagggta ggcaaaatcg tgtttacggg agggcgaacc
    11281 aaaaaaggct atatgaccga agccgaggtc gggcggcggt acgcgctcaa acagggggtt
    11341 ccggcgcgca acatcctgtt tgaaaacact tcgcgcaaca cttatgaaaa cctgaacaac
    11401 atccgtccgg ttttgcgtgc caacggtatt gccagcgtgg tgattgtcag cgatccctac
    11461 catttggcgc gggcggcgga aatggcggag gatttgggtg tccgcgttta tatgtctgcc
    11521 accccgacca cgcggtttga tgcgggaaat aagaaaaaaa tctttatgct gcaagaggga
    11581 tatgcgttat ccctctaccg tttggaaaaa tgggggtggg attttttcaa ttggctgttt
    11641 cattgaacct tttttcggac gcaatcccaa aggaacccgg tcggcggcgc gtgatacact
    11701 gttccgcgtg ccgaagcatt gaaaacaagg aaaataccgt ctgaaactta tccgcaggca
    11761 gggttcggac ggcattttta atgcccaagc acggtaaaaa cagtaaaata agccgtttta
    11821 aagattctgg agccatcatg accgtcaaaa cccgtttcgc ccccagcccc accggctacc
    11881 tgcacattgg cggcgttcgc accgccttgt tttcatgggc gtttgcccgc catcacaaag
    11941 gcgagttcct attgcgtatc gaagacaccg acttggcgcg ttccaccgcc gaatccgtca
    12001 acatcatcct cgacggcatg aaatgggtcg gactggatta cgacaacgcc gacaacgtcg
    12061 tgtaccaaac ccgccgtttc gaccgttata aagaagtcat tgccgaactc ttggcaaaag
    12121 gcgatgccta ctactgctat tgcagcaaag aagaattgga agcgatgcgc gagaaagccg
    12181 aaaaagaagg cacggcgact tacgaccgcc gctggcgtcc ggaagaaggc aaaaccctgc
    12241 ccgaaatccc tgccgacgtg caacctgttg tccgtttcaa aacacctttg gacggcgtca
    12301 cccagtggac ggacttggtc aaaggcgaaa tttccattcc caacgaagcg ctcgacgacc
    12361 tgattatcgc ccgcgctgat ggcacgccga cctacaactt ctgcgtcgtg gtggacgact
    12421 acgatatggg cgttacccac gttatccggg gcgacgacca tgtgaacaac accccgaaac
    12481 aaatcaatat cttgaaagcc atcggtgcga acctgcccga atacgggcac ctgccgatga
    12541 ttctcaacga acaaggcaaa aaaatctcca aacgcagcgg cgataccgtt gccattaccg
    12601 actttggcgc aatgggcatc ctgcccgaag ccatgctcaa ctatctggca cgcttgggct
    12661 gggcgcacgg cgacgacgag ttctttacga tggaacaatt catcgaatgg tttgatttga
    12721 aagacgtttc cccgtcccca agccgtatgg atttgaaaaa actctattgg atcaacggcg
    12781 aacacatcaa aatcacgccc aacgacaaac tcgccgaact cgtcaaaccg cgtctcgctc
    12841 tgcgtgatat tcatgaaacc gaaaaacctg cgttggaaga cgtgttggca caggttaaag
    12901 accgcgccca agatttgaac gccttggcag acgaatgcct gtatttctac aaaaaacaag
    12961 tccctgccga agccgatgtt gccaaacact gggacgacga agcctccgcc cgtatgctgc
    13021 gctttgccga acgcctcgag ggtttggaag actggaatac cgaagccatc cacgacctct
    13081 tcaaaccttt ctgcgacgaa gaaggcatca aaatgggcaa actcggcatg cccctgcgcc
    13141 tcgccgtctg cggtacggcg aaaaccccga gcgtcgatgc cgtgttggca ttaatcggca
    13201 aagaggaagt gttgaaacgc atccgcgctt aagttatcca caggcgtggg cgatgccgtc
    13261 tgatgtcgga atacggcttc agacggcatt caaacggaag ttgaacatga aaaaaatatt
    13321 gtattttctg atttttgttt tttctacaag cgtatgggcg ggggatgcgg aagacaatct
    13381 gctcagcatc caatccggct atcgtgcctt actgcaaaaa caaaacaatc tggacggaaa
    13441 aatcatcggg atgcagtcgg atttggaaga tgcgcgccgg cgtttgcaga cggctcaggc
    13501 agacatcgcc cgtttggaag cggaaattcc tgcagcaatg gcgcaaaaag cccggcaggc
    13561 tgaagagctg aggcaaatcg gagtgcgttt ggaccatgct tggaatgcgg tttacggcgc
    13621 agggggaacg aaagcgtcgg ggaattgacc gatgaattcc ctctttgtgg acaatactgt
    13681 tttcattaca cggctgaaag ccgggcatat cggcaggttg gttcaggcgt tgtttgagga
    13741 gtggcacgga tttgaaccgt ggtcttctgt ggataagatt cgtgcctatt acggcagatg
    13801 tttgaaggat gacgaactgc cgctggcatt tgcggctgtg gatgattccg aaaccctgtt
    13861 gggttcggct gcggtcaagc ggcatgatat ggaaagtttt ccacggtatg aatattggtt
    13921 gggggatgtc tttgttttac ctgaatatcg cggaaaaggc attggcagga ggctggtcgc
    13981 ccactgcata ggcgcagcgc gttcgctggg gataaagttc ttgtatcttt atacgcctga
    14041 tgtgcaaata ttttatgaat cattcggctg ggcggttgtc gggcgacatt tccataacgg
    14101 tgaatgggtt acggttatgc gtttggatgt ggataaggtt taagccgaat ggacagatgc
    14161 cgtctgagct gttcggacgg cattttttat gtttggaaat ttcctgtcag ataatgttta
    14221 tccacagatt tttccatatg gtttgatgct gttccgacaa cggaaattaa gatacggatt
    14281 tgcacaaatc tttcagacgg tacagttctg acaatgcttc gcgcggggtc aggctgtcgg
    14341 gatcgagttt ttccaaggct gctgccaata taccttcaaa atgtttttcc tctgctttgt
    14401 ccacaaagtt gcccacattc ggttcatctc ctttttcaga cggcatggta ctgaaaatat
    14461 ccagttgggg acggtttgcg gcggcttggt cttccagttc gttcaaatgc ttttgggcgg
    14521 atttcaatgc gcgtacaggc aggccggcga gtttggcgac ggcgatgccg tagcttttgc
    14581 cggcgggacc cggttggatt tggtgcagga acacgatgtc ctgtccctgt tcgagcgcgg
    14641 aaaggtgcat attgagtgcg gcggtgtggg cttcgggcag gtaggtcagc tcgaaatagt
    14701 gggtggcaaa caggctgaag gatttgtttt tttgcagcag gtgttcggca acggcgtgcg
    14761 cgagggcgag gccgtcgaaa gtggaagtac cacgtccgac ttcgtccatt aaaacaaggc
    14821 tttgttcggt ggcgtgatgc aggatgtagg cggtttcgct catttcgacc atgaaggtgg
    14881 agcggttgga ggcgagatcg tccgatgcgc cgatgcgggt gaagatttga tcgatgggtc
    14941 cgattgtggc ggcatcggca ggcacgaaac agccggtgtg tgccaataaa acaatcagcg
    15001 cgacttggcg catataggtg gatttgccgc ccatattggg gccggtgagc agcatgaggc
    15061 ggtgtttgtg gtcgaggtcg gtgtggttgg cggtgaagtg gcgtacctgc tgttcgacaa
    15121 cgggatggcg gccgttttcg atgtggataa ccggatagtc ggcaaactcg gggcggacga
    15181 agttccgctc ttttgccaag gctgaaaatg tggacaacac gtccagcgcg gcggcggctt
    15241 tggcggcttt ttgaagctgc ggcaatgccg tctgaaggtt tttcaatacg ccgtcaaaga
    15301 gttgtttttc taaggcgagg gcttgctctt gagcagtcag cactttgtct tcaaaggctt
    15361 tcaactccgg cgtgatgaag cgttcggcgt ttttgagggt ttgccggcgt tggtagtcgg
    15421 caggtgcttg ttcggcttgg gttttggaca attcaatgta aaagccgtga acgcggttga
    15481 actcgacttt gagtgtggac aaaccggtac gttcgcgttc cttggcttcc aaatccagca
    15541 aaaattcgtc gccatggttt tgaatgcggc gcaattcgtc cagttcggga tgaaaaccgt
    15601 ggttgatgac attgccgtct ttcagccaga cggaaggttc gggcaaaatg gcttggcgga
    15661 gctgttcggc tgtggatagg ttttccggga aaacggcttt gagggtttcc aacaggctgc
    15721 tgccgtttgc ggacaagtcg atttgtgcca actcgaacag actgtcgcgt aaagatgcca
    15781 aatcgcgcgg gcgggcgttg ccgacggcga tgcgggcagc aatgcgttca atgtccgcaa
    15841 tgtttttcag acggcattgg aggggtttgt attggctttc cagcgcggca acggcttctt
    15901 ggcgcgctcg gatgtgggcg cggttgcgta aagggtggtg cagccagaga gccaagaggc
    15961 ggctgcccat atgggtagcg caaaggtcga gcgtggacat cagggtcggc gattttttgc
    16021 cggagagggt ttgcgtgatt tcgagattgc ggcgcgtggc ggcatccata ccgatatatt
    16081 ggctgtcggt ttcgagcgac aggccgtcca aatgttgcgg catcaggttt tgcgtcagac
    16141 ggatatagtt caacagtgca cctgccgcgc caatcgcaac ggcgtgttct ttgccgtcca
    16201 aaccgaagcc gcgcaaatcc tggcagccga aatattccgt cagcagtttt tcccccgcgt
    16261 cggcggcaaa ctgccacgcg ttcaggcgcg taacacccga tgccgtctga agttgcggtg
    16321 cgtttttact gtcaggcaac agaatttccg ccgcctgcag gcgcgccagt tcgtcgtcca
    16381 atttatccac agttgtcagc ttggttttga attcgccgct ttgcagcgat gcccacgcca
    16441 aaccgatgta ttttttgtcg ggggacacgg caacgatgcg gttggtttcc ttgtcttcca
    16501 gcaatgcgga atcggtcagc gtgccgggcg ttacgatgcg cacgactttg cgctccaccg
    16561 gtcctttgcc tgcgccgact tcgccgacct gttcgcaaat cgccacgctt ttgcccaact
    16621 tgaccaggcg cgccagatat tgttcggcgg cgtgaaacgg cacgcctgcc attttgaccg
    16681 gctcgccatc cacctgtccg cgcgtggtca gggtaatatc caaaagtttt gccgcttcta
    16741 ccgcatcgtc gaaaaacatc tcgtaaaaat cgcccatacg gtaaaacacc agtttgtcgg
    16801 tatgttgcgc tttgatgccg aggtattgct gcatcattgg ggaaacggcg gatttggaca
    16861 tggggctgcc ttatcattag gaaaacggta ttgtagcaaa aggttttgtc ttcgacggaa
    16921 tgagactgac cgtaagcctg tcaataaggt tgtggagaca actaggaaaa cagcaaagtt
    16981 atccacagaa atattattaa gtcaaaaaac atccacgctt atctgtctta tacagcaagt
    17041 cttaaactca aacttatttg acaaaaagat aatgaaaata ttggatattt gctacttacg
    17101 cacaagcagg ttggcaatca tcaaaatcat cattaaaatt taaattcaaa tattgatggt
    17161 aaaggttttg gaaatctgtg caaacagcaa aattcatcta tatcaaataa gcgtcaagta
    17221 gttagatgta atagtggaat gtaactttat ttcagaatac aatgcaggca tcggacttta
    17281 tttcacaacc tggagacaaa atatgagcat taaagtagcg attaacggtt tcggccgcat
    17341 cggccgcctc gcattgcgtc aaattgaaaa agcccacgac atcgaagtcg tcgccgtcaa
    17401 cgacctgacc cctgccgaaa tgctgctgca ccttttcaaa tacgacagca cgcaaggccg
    17461 tttccaaggt actgccgaat taaaagacga tgccatcgtg gtaaacggca aagaaatcaa
    17521 agtttttgcc aatccgaatc ccgaagaatt gccttggggc gagttgggtg tggatgtcgt
    17581 cctcgaatgt accggcttct ttaccaacaa aaccaaagcc gaagcccaca tccgtgccgg
    17641 cgcacgcaaa gtcgtgattt ccgcacctgg cggcaatgat gtgaaaaccg tcgtatatgg
    17701 cgtgaaccaa aacattttgg acggcagcga aaccgtcatt tccgccgctt cctgcaccac
    17761 caactgcctt gccccgatgg cggcagtcct gcaaaaagag ttcggcgtag tcgaaggtct
    17821 gatgaccacc atccacgcct acaccggcga ccaaaacacc cttgacgcgc cgcaccgcaa
    17881 aggcgatttg cgccgcgccc gagccgccgc gctcaacatc gtgcccaaca gcaccggtgc
    17941 cgccaaggcc atcggtctgg ttatccccga attgaacggc aaactcgacg gctccgccca
    18001 acgcgtccct gttgccaccg gctcgctgac cgaattggtt tccatcctcg aacgccctgt
    18061 aaccaaagaa gaaatcaacg ccgcgatgaa agccgccgcc agcgaatctt acggctacaa
    18121 cgaagatcaa atcgtttctt ccgacgttgt cggcatcgaa tacggctcgc tcttcgatgc
    18181 gacccaaacc cgcgtgatga ccgtgggcgg caaacaattg gtgaaaaccg ttgcctggta
    18241 cgacaacgaa atgtcctaca cctgccaact cgtccgcact ttggaatact ttgcaggcaa
    18301 aatctaaggc ttgaacaaac ctgtggataa gtaaatgccg tctgaacaca ttgcgttcag
    18361 acggcattgt ttatctgtat
//
LOCUS       DAXHRI010000025        18291 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXHRI010000025 DAXHRI010000000
VERSION     DAXHRI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18291
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.025"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(152..487)
                     /locus_tag="ACFM75_000660"
     CDS             complement(152..487)
                     /locus_tag="ACFM75_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6255245.1"
                     /translation="MFKDWKEHTALVKKSFGELGKSHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            616..1521
                     /gene="mtrA"
                     /locus_tag="ACFM75_000661"
     CDS             616..1521
                     /gene="mtrA"
                     /locus_tag="ACFM75_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC6255246.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1583..2071)
                     /locus_tag="ACFM75_000662"
     CDS             complement(1583..2071)
                     /locus_tag="ACFM75_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6255247.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2218..3120
                     /locus_tag="ACFM75_000663"
     CDS             2218..3120
                     /locus_tag="ACFM75_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6255248.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSLSSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3161..4291)
                     /locus_tag="ACFM75_000664"
     CDS             complement(3161..4291)
                     /locus_tag="ACFM75_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6255249.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPSAENPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4496..7120
                     /gene="alaS"
                     /locus_tag="ACFM75_000665"
     CDS             4496..7120
                     /gene="alaS"
                     /locus_tag="ACFM75_000665"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC6255250.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYTERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEITEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQSGAAHAKA
                     LEKELAKAKAELAVHAGAKLLDDAKDLGAAKLVATQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPAVLDSVKDWVGAKLV"
     gene            complement(7209..8231)
                     /locus_tag="ACFM75_000666"
     CDS             complement(7209..8231)
                     /locus_tag="ACFM75_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6255251.1"
                     /translation="MLTKKEFEKFLDEVCTVLRQEAKNAPFKSQDIFENRVRALCIAK
                     IPAYADIVINPEPVPQIFPDVPIGEYGVEVKFTLKDTWRSVANSIQEKNKADGVQHIY
                     VVFGKMGGIPDVKWQLYEDSVIHVRTSHVPRFEVEIESDRPSLFEGFGISYAEFADLD
                     MHEKMEYIRKYARNRLKEGERLWWIEDIDAVDSHDLPLEVRLYTSLPQEEKIRLRAEA
                     ALVSPRIVCSGRAKHKYDNAVLYLITYRGVLCHQARDLFSAGSVANSAEDYRGGNYVE
                     KSLKLIEHEIEKAALEMDDALIVEYWGESVPTEKRLEYWIKMLDDLADGWIPSESLFN
                     GRYKAK"
     gene            complement(8285..9436)
                     /locus_tag="ACFM75_000667"
     CDS             complement(8285..9436)
                     /locus_tag="ACFM75_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6255252.1"
                     /translation="MTEFARKNWNKLAGDAIKAKMHEHGIGFIQLSELFLEQGESETA
                     NAICNKVNRGTFSHAFYLKCMELMTKQEKQEDREKKYKIISLFSGCGGMDLGFLGGFE
                     FLNRTYDKHDFDIIWANEIDEHAVRTYRHNLGNHIVHGDIWQHLDEIPTSADIIIGGF
                     PCQDISINGKGAGISGSRSGLYKAMLSAVERVRPKIFVAENVKGLLMKHHRKDLETVL
                     SDFRNLGYQVDYYLLNAADYGVPQTRERVIIVGRLPEIPEIQIEKTNTSNSYVTARQA
                     IGDLVDKVEDEIANHIWSRAEKSPDQGSRKLDAERAGYTIRAECHGNIQWHYELPRRI
                     SMREAARIQSFPDNFIFQAKLRATERQIGNAVPPVLGWHIAAAVSKALK"
     gene            10246..10476
                     /locus_tag="ACFM75_000668"
     CDS             10246..10476
                     /locus_tag="ACFM75_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255253.1"
                     /translation="MFKTLKSIWQGLTQKGKIFPYQLAFTLLIPLRNWALSPQQITQR
                     LHFVPHHRICCGAGYFSCVGCASAHRFLIFAC"
     gene            10717..11661
                     /gene="tehA"
                     /locus_tag="ACFM75_000669"
     CDS             10717..11661
                     /gene="tehA"
                     /locus_tag="ACFM75_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFC6255254.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYAGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRG"
     gene            complement(11735..12418)
                     /locus_tag="ACFM75_000670"
     CDS             complement(11735..12418)
                     /locus_tag="ACFM75_000670"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC6255255.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVIPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            12714..15017
                     /gene="parC"
                     /locus_tag="ACFM75_000671"
     CDS             12714..15017
                     /gene="parC"
                     /locus_tag="ACFM75_000671"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC6255256.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLSDENEKRKLIVKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDCLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SSGYGFITKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSELEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGRLLPISGSLKQLSSPK"
     gene            15037..16215
                     /locus_tag="ACFM75_000672"
                     /pseudo
     CDS             15037..16215
                     /locus_tag="ACFM75_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            16208..16675
                     /locus_tag="ACFM75_000673"
     CDS             16208..16675
                     /locus_tag="ACFM75_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6255257.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            16779..17531
                     /locus_tag="ACFM75_000674"
     CDS             16779..17531
                     /locus_tag="ACFM75_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6255258.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAKPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNSAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAVLLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYEADKG"
BASE COUNT         4399 a         5030 c         4519 g         4343 t
ORIGIN      
        1 aatttaatcc actatatttc acgcttaccc cttgtttctc aaatgccgtc tgaaaaaaac
       61 ggctgtatat gttgtctgca aaatatcggg aagcataaca gacaaaatgc cgtctgaaat
      121 attttcagac ggcatttcaa tccgattcgg attatttttg cgtttcaacc gcttccaatg
      181 cacgcagggc ataagtgtaa gcggcaccgg cattcagggc gatggcggtt gccaatgcgc
      241 cggcgatctc gctgtcggtc gcaccggctt tggcggcggc tgcggcatga acgctgatgc
      301 agctctcaca acgggtggta atggcaacgg cgatggcaat cagttcacgc gttttggcat
      361 ccaatgcttc ggcggcggcg gcctgttcca atgcgccgta tgcctgcagc attttaggat
      421 gggatttacc cagctcgccg aacgattttt taaccaatgc ggtatgttct ttccaatctt
      481 taaacatttt cttttccttt ctcttgcgtt taaccctgat acgcgcttgc gtatctgttt
      541 tcgatgtgcg tattattgca attattcagt tgtgtttctc gtttaatcat ctcattttat
      601 ggttcaaaaa gatttatgga cattctggac aaactggtcg atttcgccca attgacgggc
      661 agtgtggatg tgcagtgcct tttgggcgga caatggtcgg tacggcatga aaccttgcaa
      721 cgcgaaggac tggtacacat tgttacatcg ggcagcggct atctctgcat cgacggcgaa
      781 acttccccgc gtccggtcag tacaggggat attgtatttt tcccgcgcgg cttgggtcat
      841 gtgttgagcc acgacggaaa atgcggagaa agtttacaac cggatatgcg gcagcacggt
      901 gcgtttacgg tcaagcagtg cggcaacgga caggatatga gcctgttttg cgcccgtttc
      961 cgctacgaca cccacgccga tttgatgaac gggctgcctg aaaccgtttt tctgaacatt
     1021 gcccatccga gtttacagta tgtggtttca atgctgcaac tggaaagcaa aaaacctttg
     1081 acggggacgg tttccatggt caacgcattg tcgtccgtcc tgctggtgct tatcctgcgc
     1141 gcctatctcg aacaggataa ggatgtcgaa ctctcgggcg tattgaaagg ttggcaggac
     1201 aaacgtttgg gacatttaat ccaaaaggtg atagacaaac cggaagacga atggaatgtc
     1261 gacaaaatgg tggcggctgc caatatgtcg cgcgcgcaac tgatgcgccg tttcaaaagc
     1321 cgggtcggac tcagcccgca cgcctttgtg aaccatatcc gcctgcaaaa aggcgcgttg
     1381 ctgctgaaaa aaaacccaga ttcggttttg tcggtcgcac tgtcggtagg ctttcagtcg
     1441 gaaacgcact tcggcaaggc gttcaaacgg caatatcacg tttcgccggg tcaataccgg
     1501 aaagaaggcg ggcaaaaata aatcggggct tcaaacgcaa atgccgtctg aaaaggcttt
     1561 catacagcat ttgcgtaccg cgtcatttca agggctgcat cttcatcact tccatcaaaa
     1621 agttggtaaa tgcggggttg ttgggtttga catccatatt tttccaacgc tgctgccagc
     1681 cgcgcaaggc attctggata tacagcttgg actgttccgt attgattgcg ccccgctggc
     1741 tgtctatcgc cgaacgcagg tagatttcat acatactgtc atcgacggca ttgcgtccga
     1801 ccaggcgttt tctgaagttg ttcagatatt gcgccgcctg aaccttggtc attttaccga
     1861 tacccacctg atagcccaag cgcgtcgctt catcgctgat tttggcaaca tccgtccaat
     1921 gcgaagaggc aaggcggaaa ccttttgcag gtgcttccgt tttgacggta ttgataggat
     1981 tcacggggat ttccgtcaat gtgggcacat aaatagactg gcagccggaa agaactgccg
     2041 caatggaaag agggataagg tattttttca tgcccccatt ataatcaagt ttgccttgag
     2101 aaaacaaatt gttcggcaag aaaaataaaa tttcggcatc agaagcaggc aaaaacacat
     2161 tccacaagcc ttgccgcaag gtttacaatc cgaccgtcct tatcgcaacg accgtttatg
     2221 gataccgcaa aaaaagacat tttaggatcg ggctggatgc tggtggcggc ggcctgcttc
     2281 accattatga acgtattgat taaagaggca tcggcaaaat ttgccctcgg cagcggcgaa
     2341 ttggtctttt ggcgcatgct gttttcaacc gttgcgctcg gggctgccgc cgtattgcgt
     2401 cgggacacct tccgcacgcc ccattggaaa aaccacttaa accgcagtat ggtcgggacg
     2461 ggggcgatgc tgctgctgtt ttacgcggta acgcatctgc ctttggccac cggcgttacc
     2521 ctgagttaca cctcgtcgat ttttttggcg gtattttcct tcctgatttt gaaagaacgg
     2581 atttccgttt acacgcaggc ggtgctgctc cttggttttg ccggcgtggt attgctgctt
     2641 aatccctcgt tccgcagcgg tcaggaaacg gcggcactcg ccgggctggc gggcggcgcg
     2701 atgtccggct gggcgtattt gaaagtgcgc gaactgtctt tggcgggcga acccggctgg
     2761 cgcgtcgtgt tttacctttc cgtgacaggt gtggcgatgt cgtcggtttg ggcgacgctg
     2821 accggctggc acaccctgtc cctctcatcg gcggtttatc tgtcgggcat cggcgtatcc
     2881 gcgctgattg cccaactgtc gatgacgcgc gcctacaaag tcggcgacaa attcacggtt
     2941 gcctcgcttt cctatatgac cgtcgttttt tccgccctgt ctgccgcatt ttttctgggc
     3001 gaagagcttt tctggcagga aatactcggt atgtgcatca tcatcctcag cggtattttg
     3061 agcagcatcc gccccactgc cttcaaacag cggctgcaat ccctgttccg ccaaagataa
     3121 aaaatgccgt ccgaacatcc ttcagacggc atatcgggct ttatttcccc gccttcacat
     3181 cctgccactg gcgcaccata aacttcaatg ccgccggctg gataggcacc atgataaagc
     3241 tgtttttcaa atcctcctcg gttgggaaaa tcgtattgtc gtttttaaat tcgtcttcca
     3301 tcagctcacg cgccggcttg ctcgaaggcg cataagtaac gaaattgccg tttttcgccg
     3361 acacttccgg atcgaggaag tcgttgatgt atttgtgcgc gttggcgacg tttttcgcat
     3421 ctttcggaat cacgaaagaa tccacccaaa tccccacgcc ctctttgggc atcatcacgc
     3481 ggattttttc cttgccgccc gcttcttcgg cacggcgttt ggcgatgttc aaatcgccgc
     3541 cgaaaccgat tgttacgcag gtatcgccgc gcgccaaatc atcgataaag ccggacgaag
     3601 taaagcgttt gatattgggg cggtttttct tgagtagggc ggttgcctcc ctgatgtctt
     3661 ccgtattgct gctgttcggg tttttaccca aatagttcaa caccatagga tagatttccg
     3721 ccgcgctgtc caaatagctg atgccgcatt gcttgagttt ggacgtgtat tcggggtcga
     3781 acaccaaatc ccactggttg tccggcagct tgtccgtacc caaagccttt ttcacgcgtt
     3841 cggtattgat ggcgaaggta tttgtccccc aataaaacgg cacggcgtat tcgtggccgg
     3901 gatcgacccc gtccatcagc ctcatcattt cgggattgag gtgtttataa ttgggaatca
     3961 gcgacttatc gattttctga tacgcacctg ccttaatctg cctgcccaca aacgcattgg
     4021 acggcgcgac aatgtcgtaa ccggacttgc ctgtcagcac cttgctttcc agcgtttcat
     4081 cgctgtcgta cacatcataa gtaaccttga tgccgttttt cttttcaaaa tcggcaacgg
     4141 tttccggatc gacatattcc gaccagttgt aaattttcaa tacgttttgg ttttccgccg
     4201 gtgccggatt ttcggcagat ggtttgtccg aaccgccgca cgctgcaagc agcaaagcag
     4261 tcaggacggc gagtggcaga tgtttggcca ttatcattcc ttgcatatcg ggttggagaa
     4321 agcggccatt atagccgata ttggcaacag ggcttcagac ggcattcaaa atcccgccac
     4381 acccttccga aaaccgccgc ttccatagct agaaacaggg atttgcggta agataccgcc
     4441 gttcgttttc cctgctttta ccatgacaag acatttgaga gacattgaaa aaattatgaa
     4501 aacctccgaa ctgcgccaaa aattcctaaa attttttgaa accaaaggcc acaccgtcgt
     4561 ccgctcttcc agcctcgtgc cgcacgacga cccgaccctg ctgtttacca acgcgggcat
     4621 gaaccagttt aaagacgtat ttttaggttt cgacaaacgc ccgtacagcc gtgccaccac
     4681 cgcgcaaaaa tgcgtacgcg ccggcggcaa acacaacgac ttggaaaacg tcggctacac
     4741 cgcccgccac cacaccttct ttgaaatgat gggcaacttc tccttcggcg actacttcaa
     4801 acgcgacgcc atccatttcg cttgggagtt tctgacttcc cccgaatggc tcaacatccc
     4861 taaagacaaa ctgttggcga ccgtttacgc agaagacgac gaagcctaca acatctggtt
     4921 gaacgaaatc ggtatgccgt ctgaacgcat cgtccgcatc ggcgacaaca aaggcgcgaa
     4981 atacgcgtcc gacaacttct ggcaaatggg cgacaccggt ccttgcggcc cttgttccga
     5041 aattttctac gaccacggtg aagaaatctg gggcggcatt cccggcagcc ccgaagaaga
     5101 cggcgaccgc tggattgaaa tttggaactg cgtatttatg cagttcaacc gcgacgaaca
     5161 aggcaatatg aacccgctgc ccaagccttc cgttgatacc ggcatgggct tggagcgtat
     5221 ggccgccgtc atgcagcatg ttcacagcaa ctacgaaatc gacttgttcc aagacctgct
     5281 caaagccgtt gcccgcgaaa ccggcgcagc tttcagcatg gatgaaccca gcctgaaagt
     5341 catcgccgac cacatccgct cctgctcatt cctgattgca gacggcgtct tgccttccaa
     5401 cgaaggccgc ggctacgtat tgcgccgcat tatccgccgc gccgtgcgcc acggttacaa
     5461 actgggtcaa agcaaaccgt tcttccacaa actggtggcc gatttggtca aagagatggg
     5521 cggtgcctac cctgagttga aagagaaaca agcacaaatc gaagaagcgt tgaaaaacga
     5581 agaaagccgt tttgcccaaa cgctggaaac tggtatggct ttgttggaaa acgcgctggc
     5641 caaaggcggc aaaacactcg acggcgaaat catcttcaaa ctctacgaca cctacggctt
     5701 cccatacgac ttgactgccg acatctgccg cgaacgcaat atcgaaccgg acgaagcagg
     5761 cttcgagcgc gaaatggaag cccaacgcgc acgcgcacgc gccgcccaaa gcttcaaagc
     5821 caacgcccaa ctgccttatg acggtcaaga caccgagttt aaaggttata ccgaacgcca
     5881 aaccgaatcc aaagtcctcg ccctctacaa agacggcgag caagtcgacg aattgaacga
     5941 aggcgacagc ggcgcagtcg ttatcgactt taccccgttc tatgcagaat ccggcggcca
     6001 agtcggcgac gtcggctata tctttgcagg cgaaaaccgc tttgaagtac gcgataccca
     6061 aaaaatcaaa gcggccgtat tcggacaatt cggcgtacaa acttcaggcc gtctgaaagt
     6121 cggcgacagc gttaccgcca aagtggacga cgaaatccgc aatgccaata tgcgcaacca
     6181 cagcgcaacc cacttgatgc acaaagccct gcgcgatgta ttgggcagac acgtcgaaca
     6241 aaaaggctct ttggttaccg ccgaatccac ccgtttcgac atttcccatc cccaagcggt
     6301 aactgccgaa gaaatcaccg aagtcgaacg ccgcgtcaac gaagccgttt tggcgaacgt
     6361 tgccgtcaac gccgccatta tgagcatgga agacgcgcaa aaaaccggcg ccatgatgct
     6421 cttcggcgaa aaatacggcg acgaagtacg cgtattgcaa atgggcggtt tctctaccga
     6481 attgtgcggc ggcacgcacg tttcacgcac cggcgacatc ggcctcttca aaatcatcag
     6541 cgaaggcggt attgccgcag gcgtgcgccg tatcgaagcc atcaccggcc tgaacgcact
     6601 caaatgggcg caagagcaag agcgtttggt gaaagacatc attgctgaaa ccaaagccca
     6661 aaccgaaaaa gacgtactgg cgaaaatcca atcaggcgcg gcacacgcca aagccttgga
     6721 aaaagagttg gcaaaagcca aagccgaact cgccgtccac gcaggcgcaa aactcttgga
     6781 cgacgcaaaa gacttgggcg cagccaaact cgttgctacc caaatcgaag ccgacgcagc
     6841 cgccctgcgc gaaatcgtaa ccgacctgac cggcaaatcc gacaacgccg tgattctttt
     6901 ggcggcagtc aacgacggca aagtctccct gtgcgcaggc gtatccaaag ccctgaccgg
     6961 caaagtcaaa gcaggcgatc tggttaaatt tgcagccgaa caagtcggcg gcaaaggcgg
     7021 cggccgtccc gacttggcgc aggccggcgg cacggatgcc ggcaaattgc ccgccgtgtt
     7081 ggatagcgtg aaagactggg tcggcgcgaa gctggtttga tgtgggaaag gcagcctgaa
     7141 agggattcag gctgcctttt gtgttaaaga ggccgtctga aagatatttt caaacggcct
     7201 ctgtcagttt atttggcttt atacctacca ttgaatagcg attcagaagg tatccagcca
     7261 tctgccaaat catccaacat cttaatccaa tactctaggc gtttttctgt cgggacgctt
     7321 tctccccaat attcgacaat tagagcatca tccatttcca aagcagcttt ttcaatttca
     7381 tgctcaatca attttaaaga tttttctaca tagtttccgc cacggtagtc ttccgccgag
     7441 ttggcaacgc tgcccgcact gaataagtcc cgcgcctgat gacaaagcac accgcgatag
     7501 gtaatcagat acaggacggc attatcgtat ttgtgcttcg ccctgcctga gcagacaata
     7561 cggggggaga ctaatgctgc ttcagctcgg aggcggattt tttcttcctg cggcagggaa
     7621 gtgtaaaggc gcacttccaa aggcagatca tggctgtcta ctgcgtcaat gtcttctatc
     7681 caccataacc gctcgccttc tttcaacctg ttacgggcat attttcggat atattccatt
     7741 ttttcatgca tatccaaatc ggcaaattca gcataggaaa taccgaaacc ctcaaacaga
     7801 gacggtcgat cagattctat ttctacctca aagcgcggaa catgcgatgt gcggacatgg
     7861 attacgctat cttcgtaaag ttgccatttc acatcgggaa tgccccccat ttttccgaat
     7921 accacataga tatgctgtac accgtcagct ttgtttttct cttggatact gtttgctacg
     7981 ctgcgccaag tatcttttag cgtgaatttg acttctacgc catattcccc tattggaaca
     8041 tcggggaaaa tttgcggcac aggttcagga ttgataacta tgtcagcata agcgggaatt
     8101 tttgcaatgc acagcgcacg aactctgttt tcaaaaatat cttgggattt gaaaggtgcg
     8161 ttttttgcct cttgcctcaa tacggtgcaa acctcgtcta aaaacttctc aaactccttt
     8221 tttgtcagca taatgcatta ccacttaaaa atataaagta gtaattttat cttatttaac
     8281 agggttattt aagtgcctta ctaacggcgg cggcgatgtg ccaccctaaa acaggaggga
     8341 cggcgttgcc aatttgccgt tctgtggcac gcaattttgc ctggaaaata aaattgtcgg
     8401 gaaacgattg gatgcgcgcc gcctcacgca tggagatgcg gcggggtaat tcgtaatgcc
     8461 actgtatgtt tccatggcat tctgccctaa tagtgtagcc cgccctttcg gcatccaatt
     8521 ttcgactacc ttgatcgggg cttttttctg ctctactcca aatatggttg gcaatttcgt
     8581 cttctacctt gtcgaccaaa tcgcctatag cctgtctagc tgttacatag ctgttgctgg
     8641 tatttgtttt ttcaatctgt atttcgggaa tttcaggtag ccttccaacg atgataactc
     8701 gctcccgagt ctgtggaaca ccgtaatcgg ctgcattcag caagtagtaa tctacctgat
     8761 aacccaaatt gcgaaaatct gaaagcactg tttccaaatc tttccgatga tgcttcatca
     8821 gtagcccttt gacattttca gcaacaaata ttttggggcg aacgcgttca acagcagaca
     8881 acatggcttt atacagcccg ctgcggctgc ctgaaatacc tgcaccttta ccattaatag
     8941 aaatatcctg acaaggaaag ccaccgataa tgatgtctgc gctagtgggt atttcatcca
     9001 aatgctgcca aatatcacca tgtacaatgt gatttcctaa gttgtggcgg taggttctaa
     9061 cagcgtgttc gtcgatttcg ttcgcccaaa taatatcaaa atcatgtttg tcataagtac
     9121 ggtttaaaaa ctcaaagccc cccagaaaac ccaaatccat tccgccacaa ccgctaaata
     9181 aagaaataat tttatatttt ttttcgcggt cttcttgctt ttcctgtttg gtcattaact
     9241 ccatacattt aagataaaaa gcatgggaaa aagtacctct atttactttg ttacaaattg
     9301 catttgccgt ttcactttcc ccttgctcca agaataattc tgataattgt ataaagccaa
     9361 ttccatgctc atgcattttg gcttttatgg catctccagc taacttgttc caattttttc
     9421 gtgcaaattc ggtcatggtt aatctttcag tcattcataa atgacattat tttagcgttt
     9481 tttataattt aagccaagat ttaaaatcta cctttaggtt tacaggccgt ctgaaaacaa
     9541 aaatctcgtt ttcagacgac ctttttccgc taaaatcccc ctaccgtcca acccgaaaca
     9601 aggagaacca tcatgcaagt tcctacttcc gccatcatca acggcgaatt tgccgacaaa
     9661 tacggcaaac gcggcagcaa gccgtagcca gcacccctgt caaccgcgtg cgtggctaga
     9721 ccacacaccc taccactgcg ggcgacgcaa accttaagat tgaggtgtag ggtgtgtgcg
     9781 gtacgcacgc acgcggtttt tgttactacc gaatgattcc aaaccatgcc gtaatccagt
     9841 cagcagcaaa cgcagcttgg gtcatgtacc aacaaaactc aatatcaaag gccgtctgaa
     9901 gaataatttt cagacggctt ctctctatca aaagcagcct gcacattcaa aaatcgaagt
     9961 gcaggctgct ttttgtgatg attggaaaaa acacacacta tttgagacct ttgcaaaaat
    10021 agtctgttaa cgaaatttga cgcataaaaa tgcgccaaaa aaattttcaa ttgcctaaaa
    10081 ccttcctaat attgagcaaa aagtaggaaa aatcagaaaa gttttgtatt ttgaaaatga
    10141 gattgagcat aaaattttag taacctatgt tattgcaaag gtctctattt ttatactgta
    10201 tattgtgcag gtacaaagta aaacgaatta cccaaagaaa ttcctatgtt taagacgctc
    10261 aaaagcattt ggcagggact tacgcaaaaa ggcaaaatct tcccgtacca actggcattc
    10321 acgctgctga ttcctttgcg taactgggcg ctttcaccgc aacaaatcac gcaacggctg
    10381 cactttgttc cgcaccaccg aatttgctgc ggggcgggtt atttcagttg cgtaggatgt
    10441 gcttcagccc accgtttcct catttttgct tgttaattgg tgggctgaag cccacactac
    10501 ggttaatacg gttactgatt cgtttccccc tgcatccgaa tcacgccgca caccgcaaaa
    10561 aacgcgcgcc cgatgcgcaa ccgcttccga catctggcgc aatgagacaa aacggcaata
    10621 tgccgatacg gatacgtcat cctccgcctg cctgtttttt atatcatact caattttatc
    10681 tgtgcgttac gacggcatgc cgtctgaagg gtgtttatgt ctgcatctaa gaaatttccg
    10741 attcctttga gctatttcag catcgcgctg ggcttgtttg ccttggggct gtcgtggcgt
    10801 tacggcgcgt ctgtcgggct gctgcccgcc ttggccgccg aatcgctgct tgcggcggct
    10861 tcggtcgtct ggctcttgct ggtggcggca tacctgatca aaatgtttgc gtaccgaaac
    10921 gattttttgt ctgatttgcg cgacttggtg caatgctgct tcatcagcgc gattccgatt
    10981 accgctatgc tggagggact cgcgctgaag ccctatcagg caggcgcggc ggcagtcctg
    11041 atttatgccg gcgttgccgg acagttggcg ttttcgatgt atcgggcggc cggtctgtgg
    11101 cgcggcctgc attccttgga ggcgacgacg ccgattattt atctgcctac ggttgcgaca
    11161 aactttgtca gcgcgtcatc tctggccgcg ttggggcatc atgattatgc agctttgttt
    11221 ttcggcgcgg gtatgttttc ctggctgagc ttggaagcct ccatcttggg caggctgcgc
    11281 acggcggcac cggtcggcac ggcggcgcgc ggcgtggtcg gcatccagct tgcgcccgcc
    11341 tttgtcggct gcggcgcgta ttttgccgtc ggcggtaaag tcgacggttt tgcgttggca
    11401 ttaatcggct acggctgcct gcagcttttg ttcttgctgc gcctgacccg ctggttttgg
    11461 gaaggcggtt ttacgatgag cttttgggga ttttcattcg gtttcgcggc aatggcagga
    11521 tgcggtctgc atctggcggc ttctggcgta ttgtcgggct tggggctgac gcttgccacc
    11581 gccggatcgg caggcgtggc gctgctgctt gtcggtacgc tgcaccggat agcgacgggg
    11641 cgtttcttgg tacgcggctg atgcgttttg ccgccttgtc aaaaatgccg tctgaacgct
    11701 caagccttca gacggcattt tttatttcac acccttacag gtagaatttt tcgatgactt
    11761 tcaaattgtc gtccaatttg tacaccaacg gctggccagt cgggatttcc aagcccatga
    11821 tgtctttgtc ggaaatgccc tcgatgtgtt ttgccagcgc gcgcagggag ttgccgtgcg
    11881 ccgccaccaa gacgcgtttg ccgctcaaaa tcgcgggggc gatttggtct tcccaaaacg
    11941 gcaatacgcg ctccaaagta actttcaaat tttcgccgtc ggggataaca tcggcaggca
    12001 gatgggcata gcggcggtct ttgtgtgcgg aaaactcatc gtctttgtcc aaaagcggcg
    12061 gcagggtgtc gtagctgcgc cgccagatgc ggacttgctc gtcgccgtat tgttcggcgg
    12121 tttgtttttt gtccaggcct tgcagtcggc cgtagtggcg ttcgttcagc cgccatgttt
    12181 tgatttgcgg tacgaacagt tggtcggatt cttccaaaac gatgttgcag gtcttaatcg
    12241 cgcgggtcag gacggatgtg aaggcgatgt cgaactcata gccgttttct ttcagttttt
    12301 taccggcggc agcagcctcg gcaagcccct gctcgctcag cttcacgtcg cgccagcctg
    12361 taaacaggtt tttcgcgttc cattcgcttt gtccgtggcg gataaatacc aattccatat
    12421 cgtctccaat gtgtgaaagt gggaaagcct tatttataac atattttcac attcccgtat
    12481 ttgattcaga ttcagacacg cgcccactat ggtttgccgt tttgatttac aataatgccc
    12541 tttgctttac attccgcata cacaatgttt caacacacag gacgacacat aaagcaccgc
    12601 cctatgtgtt gccctgattt ggaaggggtt gccccctccc aaataaagtc tgattctacc
    12661 gccccgaagg acagatgtcc aagtggcggg ggttcaaccg aaaaggaaat acgataaata
    12721 tgcaaccgca cgctccacat accgattcca atacgctgat gctcggccga tacgccgaac
    12781 gcgcctatct cgaatacgcc atgagcgtgg tcaaaggccg cgcgctgcct gaagtttcag
    12841 acggccaaaa acccgtgcag cggcgcattt tgtttgcgat gcgcgatatg ggtttgacgg
    12901 cgggggcgaa gccggtgaaa tcggcgcgcg tggtcggcga gattttgggc aaataccacc
    12961 cgcacggcga cagttccgcc tatgaggcga tggtgcggat ggcgcaggat tttaccttgc
    13021 gctacccctt gatcgacggc atcggcaact tcggttcgcg cgacggcgac ggggcggcgg
    13081 cgatgcgtta caccgaggcg cgtctcacgc cgattgcgga attgctgttg tccgaaatca
    13141 atcaggggac ggtggatttc gtgccgaact acgacggcgc gtttgacgaa ccgctgcacc
    13201 tgcccgcccg cctgcctatg gtgttgctca acggcgcgtc aggcattgcg gtgggcatgg
    13261 cgaccgagat tccgtcgcac aatttgaacg aagttacgca ggcggcgatt gcgttgttga
    13321 aaaagccgac gctggaaacc gccgacctga tgcaatacat tcccgcacct gattttgccg
    13381 gcggcggtca aatcatcacg ccggcggacg aattgcgccg gatttatgaa accggcaagg
    13441 gcagcgtgcg cgtgcgtgcg cgttatgaaa tcgaaaaatt ggcgcgcgga caatggcgcg
    13501 tcatcgtaac cgagctaccg ccgaacgcca attccgccaa aatccttgcc gaaatcgaag
    13561 agcagaccaa cccgaaaccg aaagcgggca aaaagcagct caaccaagac cagctcaata
    13621 ccaaaaagct gatgctggat ttaatcgacc gcgtgcgcga cgagtccgac ggcgaacatc
    13681 ccgtgcgcct tgtatttgaa ccaaaatcca gccgcatcga taccgatacc ttcatcaaca
    13741 cgctgatggc gcaaacttcg ctggaaggca atgtgtccat gaacttggtg atgatggggc
    13801 tggacaaccg ccccgcgcag aaaaacctga aaacgatttt gcaggaatgg ctggatttcc
    13861 gcaccgtaac cgtaacacgc cgtctgaaat tccgtttgaa ccaagtggaa aaacggctgc
    13921 acatcctcga aggccgtctg aaagtctttc tgcacatcga cgaagtgatt aaagtcatcc
    13981 gcgaatcaga tgacccgaaa gccgatttga tggcggcgtt cgggctgacc gaaatccaag
    14041 ccgaagacat tttggaaatc cgcctgcgcc agttggcgcg tttggagggt ttcaaactcg
    14101 aaaaagaatt gaacgagttg cgcgaggaac aaggccgtct gaacatcctt ttgagcgacg
    14161 aaaacgaaaa acgcaagctg attgtcaaag agatgcaggc ggatatgaaa caatacggcg
    14221 acgcgcgacg cacgctggtg gaagaggccg gacgcgccgt gctgacgcag accaccgccg
    14281 acgaacccat cacgctgatc ctgtcggaaa aaggctggat acgcagccgc gccggacaca
    14341 atctcgattt gagccaaacc gcgttcaaag aaggcgactg cctcaaacaa accctcgaag
    14401 gcagaacggt tttacccgtc gtcatcctcg attcatcggg cagaacctac acgctcgatg
    14461 ccgccgaaat ccccggaggg cgcggcgacg gcgtaccggt ttcctcctta atcgagctgc
    14521 aaaacggcgc gaaacccgtt gcgatgttga caggattgcc ggaacaacat tatttattat
    14581 caagcagcag cggctatggc ttcatcacca agctgggcga tatggtcggg cgcgtgaaag
    14641 cgggcaaagt ggtgatgacc gcagacagcg gcgaaaccgt tttgccgccg gttgccgtct
    14701 atgcctcctc gttcatcaac cccgactgca aaatcattgc cgccaccagt caaaaccgcg
    14761 ccctcgcctt ccccatcggc gaattgaaaa ttatggcgaa aggcaaaggg ctgcaaatca
    14821 tcggattaaa cgccggcgaa tcgatgacgc ataccgccgt ttcttccgag ctggaaatcc
    14881 tgattgaaag cgaaggcagg cgcggcgcgg cgcacaaaga ccgcatcccc atctccctgc
    14941 ttgaggcaaa acgcggcaaa aaaggcagac tattgcccat atcgggcagc ctgaaacagc
    15001 tttcttcccc taaataaacc cggttccgca catattatgg tgatttccaa cccccgcgaa
    15061 cttgaaaaac tcaaagaccg gattcccaat ctgatcaaca tcatccgcgt cgccatcgtt
    15121 tttccgctga tgattatgca catcctcggg ctggaaaccg gcagccgtgc gaacctgcac
    15181 gcttcgtgga cggcgtgggc gttttatgtt tggctcgcca ttgcctgctg gctgattttc
    15241 ttttccatca tccatccgca ttggcaatgg caggctttga gaataccgag tttcagcgcg
    15301 gtagcggaca tcacgatgat cggcgtgctg acctacctgt tcggcggcat cgactccggc
    15361 ttcggcatcc tgattctgcc cttcgtcgtc tgctcctgcc tgctcagcta cgggcgttac
    15421 cccctgctct atgccagcta cgccgccatc ctgctgatat tcaacgccat tgccgacggc
    15481 gatatcggca aatatccgca catatcggat gcccgaaccg cctcggtaac cttcatcctt
    15541 gtcgccgcct cctatctttc cgccatcttc acctcactgt cggtcagata catcgacagc
    15601 gccggccggc tggcacgcga caaccacctc gcctaccggc gcatcaaagg ctgcgggggc
    15661 ggcagatccg ttcaacgcca aattgtgcaa aatcatcgac ggcaacgtct gccgtatcga
    15721 caaaatgctc gaagatattt cctcgccaac aagcgcaaca aaaccgaacg cgaaaccatc
    15781 ggcctgatac cgttttggga agaattcaaa caagagttcc tgctcggcca tcccgatgcc
    15841 gccggctgca tccgcctaga tatgcagggc aatcacctga ctgcctattt cgatcctgcc
    15901 cacctgcggc aaattatgtg gaacctcgcc aacaacgcgt ggcggcacag cagcaaacgt
    15961 aacggctcga ttgccgtcgt cgcccgtcct gcacaaaaaa acaccatcag catcctgttt
    16021 gccgacgacg gcggcggcgt tccgcccgaa gtgcaggaac acttgttcga acccttttac
    16081 accacggcgg aaaacggcac cggcctcggg ctgtatgtcg cccgcgaact ggcgcacgcc
    16141 aatttcggcg atttgaccta cctaccggaa gccaaatgtt tcgaactcac attaccggaa
    16201 aaaaccaatg actgaactgc aacaccccgt cctcgtcgtc gatgacgaaa ccgacattct
    16261 cgacctgatg gaaatgaccc tgatgaaaat gggcttgcgc gtccataccg cgtcaggcgt
    16321 tgccgaagcc aaaaacaagc tcgacagcca acgctattcg ctcgtcctga ccgatatgcg
    16381 tatgccggac ggctcggggc tggaagtcgt ccaacacatc aacagccgcc tgctcgatac
    16441 gccggttgcc gtcatcaccg ccttcggcaa cgccgatcag gcacaggaag cgttgcgttg
    16501 cggcgcgttc gaccccgata ccatgcagat acaggactat ctcgaccaaa tcgaacgcga
    16561 catcatcgaa caaaccctca aacaaaccga aggcaaccgc acgcaggccg ccaaacgctt
    16621 gggcatcagc ttccgttcca tgcgctaccg tatggaacgc ctcaacatcg gctgacgaca
    16681 aaacggcatc cgcaccatct ccgcccaccc gaaaaaatgc cgtctgaaac ggcacgggaa
    16741 agcgggttcg ccccacgccc gaacggacac aaaacaccat gaccgacatc cttattgaca
    16801 acaccgccac cgaaaccgtc cgcaccctga tacgggcatt cccccttgtg cccgtttccc
    16861 aaccgcccga acaaggcagt tacctccttg ccgaacacga taccgtcagc ctcaggcttg
    16921 tcggggaaaa aagcagcgtc atcgtcgatt ttgcctccgg cgcggcacaa taccggcgca
    16981 caaaaggcgg gggcgaactc atcgccaaag ccgtcaacca taccgccaaa ccgaccgttt
    17041 gggacgcgac cgcaggattg gggcgcgaca gcttcgtcct cgcctcgctc gggctgaccg
    17101 ttaccgcctt cgagcaacat cccgccgtcg cctgcctgct ttcagacggc atccgccgcg
    17161 ccctcctcaa tcccgaaacg caagacaccg ccgcgcgcat caccctgcat ttcggcaact
    17221 ccgcagtaca aatgcccgcc cttgttcaaa cacaaggcaa acccgacatc gtctatctcg
    17281 accccatgta tcccgaacgc cgcaaaagtg ccgccgttaa aaaagaaatg acctacttcc
    17341 accggctcgt cggcgaggcg caagatgagg ccgtcctcct ccataccgcc cgacagactg
    17401 caaaaaaacg cgtcgtcgtc aaacgccccc gcctgggaga acaccttgcc ggacaagccc
    17461 ccgcatacca atacacaggc aaaagcaccc gcttcgacgt ttacctgccc tacgaagcgg
    17521 acaagggata acgcccataa aacaagacac cgaaaaattt gccgttctta tgcaaacgag
    17581 aaaccggttt ttgcgtttcg actgttttgg ataagtcatc acaccttaaa gtttgtcatt
    17641 cccacggaag tgggaatcta gaaatgaaag gcaacaggaa tttatcgtaa atgactgaaa
    17701 ccgaacggac tagattcccg cctgcgcggg aatgacgggg cgggcagatg ccgtctgaaa
    17761 ttccgtcatt cccgtgaaaa cgggaatcta gaacttctga tttttcagac gacttttgaa
    17821 cattgccgcc acccaatgat ctggattccc acctgcgcgg gaatgacgag gtttcaggtt
    17881 gctgttttta agttgctgtt tcgggttgct gttttttatg gaaatgacaa ggttttagat
    17941 tgcgagaatt tatccgctcc tccgtcattc ccacggaagt gggaatccag aaatgaaaag
    18001 caacaggaat ttatcgtaaa tgaccgaaac cgaacggact agattcccgc ctgcgcggga
    18061 atgacggggc gggcggatgc cgtctgaaat tccgtcattc ccgtaaaaac gggaatctag
    18121 aacttctgat ttttcagacg acttttgaac attgccgcta cccaatgatc tggattccca
    18181 cctgcgcggg aatgacgatg taaaattatc cgggattcaa aaagacaggc tttcgcatcc
    18241 gtgggaatga ctgcggaaag atgattttta tagtggatta aatttaaatc a
//
LOCUS       DAXHRI010000026        17999 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXHRI010000026 DAXHRI010000000
VERSION     DAXHRI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17999
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.026"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..658
                     /locus_tag="ACFM75_000675"
     CDS             <1..658
                     /locus_tag="ACFM75_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6255259.1"
                     /translation="AAERITHDYPKPTGANNTSTVSDYFRNIRAHSIHPRVSVGYDFG
                     GWRIAADYASYRKWNNNKYSVNTKVLKETQDNWQELKTENQENGSFHAASSLGLSAIY
                     DFKLNDKFKPYIGARVAYGHVKHQVHSVETKTTTVTSKPNGKNLKGGPVSSREPSKPP
                     YHESHSTSSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF
                     "
     gene            complement(632..841)
                     /locus_tag="ACFM75_000676"
     CDS             complement(632..841)
                     /locus_tag="ACFM75_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255260.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKCA
                     GKGLMPSEGSDGIGIGESEVVAHAQ"
     gene            complement(859..1416)
                     /locus_tag="ACFM75_000677"
     CDS             complement(859..1416)
                     /locus_tag="ACFM75_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6255261.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFIVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMSPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1492..3126)
                     /locus_tag="ACFM75_000678"
     CDS             complement(1492..3126)
                     /locus_tag="ACFM75_000678"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6255262.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGDIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3196..3532)
                     /locus_tag="ACFM75_000679"
                     /pseudo
     CDS             complement(3196..3532)
                     /locus_tag="ACFM75_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3532..4638)
                     /gene="serC"
                     /locus_tag="ACFM75_000680"
     CDS             complement(3532..4638)
                     /gene="serC"
                     /locus_tag="ACFM75_000680"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC6255263.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            5003..5434
                     /gene="rimP"
                     /locus_tag="ACFM75_000681"
     CDS             5003..5434
                     /gene="rimP"
                     /locus_tag="ACFM75_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC6255264.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5462..6964
                     /gene="nusA"
                     /locus_tag="ACFM75_000682"
     CDS             5462..6964
                     /gene="nusA"
                     /locus_tag="ACFM75_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC6255265.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDYLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELSVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6977..9865
                     /gene="infB"
                     /locus_tag="ACFM75_000683"
     CDS             6977..9865
                     /gene="infB"
                     /locus_tag="ACFM75_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC6255266.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKNGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKT
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILMVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(10076..11470)
                     /locus_tag="ACFM75_000684"
     CDS             complement(10076..11470)
                     /locus_tag="ACFM75_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC6255267.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11544..12884)
                     /locus_tag="ACFM75_000685"
     CDS             complement(11544..12884)
                     /locus_tag="ACFM75_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC6255268.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRLVEMLAWLPS
                     KLGFPVPDARAVVEGRLNGNIADAQAWSGLLVGSIACYGILPRFLAWVVCKILLKTSK
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            13027..13653
                     /locus_tag="ACFM75_000686"
     CDS             13027..13653
                     /locus_tag="ACFM75_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC6255269.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALISLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13717..15135)
                     /locus_tag="ACFM75_000687"
     CDS             complement(13717..15135)
                     /locus_tag="ACFM75_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6255270.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGNKDDKSLSQFQALAVAISAQIGTGNVAGVSTAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            15462..16190
                     /locus_tag="ACFM75_000688"
     CDS             15462..16190
                     /locus_tag="ACFM75_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC6255271.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVANDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16249..16737)
                     /locus_tag="ACFM75_000689"
     CDS             complement(16249..16737)
                     /locus_tag="ACFM75_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC6255272.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAIVLMCRPRRKAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGIALPVWSVAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(17495..17959)
                     /locus_tag="ACFM75_000690"
     CDS             complement(17495..17959)
                     /locus_tag="ACFM75_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC6255273.1"
                     /translation="MKELDKIDFRILKILQQNARIPMMELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4755 a         5036 c         4527 g         3681 t
ORIGIN      
        1 cgccgccgaa cgtattaccc acgattatcc gaaaccaacc ggtgcaaaca acacaagcac
       61 agtaagcgat tatttcagaa acatccgtgc gcattccatc caccctcggg tgtcggtcgg
      121 ctacgatttc ggcggctgga ggatagcggc agattatgcc agttacagaa aatggaacaa
      181 caataaatat tccgtcaaca caaaagtgtt gaaagaaacc caggacaact ggcaagaact
      241 gaagacggaa aatcaggaaa acggcagctt ccacgccgct tcttctctcg gcttatccgc
      301 catttacgat ttcaaactca acgataaatt caaaccctat atcggtgcgc gcgtcgccta
      361 cggacacgtt aaacatcagg ttcattcggt ggaaacaaaa accacgactg ttacctctaa
      421 accaaacgga aaaaatctaa agggaggacc tgtctcatca agagagccca gcaaacctcc
      481 ctatcacgaa agccacagca ccagcagctt gggtcttggt gtcatcgccg gtgtcggttt
      541 cgacatcacg cccaagctga ccttggacac cggataccgc taccacaact ggggacgctt
      601 ggaaaacacc cgattcaaaa cccacgaagt ctcattgggc atgcgctacc acttctgatt
      661 ccccgatacc gatgccgtct gaaccttcag acggcatgag acctttgcct gcgcacttgg
      721 tgcgctggtc gcctccgaac atggcgcgac acccgacatt tccgccgaac gcatcgggcg
      781 tttcatgaat ccggtttaaa acgcatggaa aaatgccgtc tgaaagcctt tcagacggca
      841 ttttgcttga gattccgttt accaatgact gacaaacgct tccaaatcgg tattcttggg
      901 cttatgcact tcctctgtcg gcgtgccgac catcatcagc ccgatgattt tatccttatc
      961 cgcacatccg aaagcctcac gcaacagggg gctgttgacc cacatccccg taatccagac
     1021 attgtcgaaa ccttgcgccg ttgccgccag ttgcagcgca tacgccgcac aacccgccgt
     1081 cagcatctgc tcccattccg gtttcggctt aggcacatcg cggttcggcg caaacgttac
     1141 cccgataacc atcggcgaca tattgcccac tttttccgcc tttttcatcg catcgtcgcc
     1201 gaaattcaat tcggcaaccg tttgctttaa cacatcgcga aaacgttgca atcctacctc
     1261 gccttgaatc acgataaaac ggaaggggcg catattgccg tgatcgggaa cttgggttgc
     1321 cgcctgaaat atttgttcca actccgccgc atcgggggcg gggggcttca gctttttgga
     1381 agatcggcgg ttcgtcaata attttaaagc atccatatcg ttatactccg gtcatcggtc
     1441 gggtgttcgg acacatatgc cgtccgaagg cttcagacgg catatccggc atcagcgcgg
     1501 acggcggcag gctgccaata tatccatttc cttccgatag gtttgactgt tggaaatgtc
     1561 catcagcccc aataccgtgc tgaaatagtg gtcgtgcgaa tattcgtttt ccgccgcttt
     1621 ttgtttgagg cattggaaat ctatgtcccc gtgttggcgg aaggctttgg aaaaccacat
     1681 aaccatcggg atatgcgtct gcccagaagg cgcgatggcg taaggcgcgg cgtgcaggta
     1741 catcccgttt tcgcccaaac tttcgccgtg gtcggaaaca taatgcacca cgctttccaa
     1801 atcgtcgcgg ttttcaagtt tgcggataac cttgtcgata aactggtcca catacaaaac
     1861 cgtattgtcg taagtgttga ccagcgtggc gcgggtgcat ttgttgattt cgttggtgtc
     1921 gcaggtcggc gtgaatttgc gttcggcttc ggtatagcgt tcgtaatacg tcggcccgtg
     1981 gctgccgatg gtatgcagga ttaaaaccgc gtctttatcg tttttgttga ggacttcgtc
     2041 gaacttggtc agcaggatat tgtcgaggca ctcgccgttg cggcagtatt cgggcaggtt
     2101 gagcgaggta acgtcggtat tcggcacttt gccgcacacg cccttgcagc cggaatcgtt
     2161 ttccaaccag gtaacttcca cgccggcgcg ctgcacgatg tccagcaggt tgtcttggtg
     2221 ttcggctttg atttcgtcat aatccgtgcg gtcgaaggtt gagaacatac acggcaggga
     2281 gtgcgcggtc gatgtgccgc agcttctgac ctgcgggaaa ttgacaattt catcgccgcg
     2341 cgcggcaagc agcggcgtag tttggcggct gtaaccgttc aaaccccagt tggcggcacg
     2401 cgtggtctcg cccacgacca gcaccacgaa acggcgcagg ctgccggccg ggcggttttg
     2461 cacgaccgcc atatccaatt gcgtataagg aatattggaa cgcttccaat ctttgtattt
     2521 cgacacgccc gcgccgatga aattagacgg cacaatcaga tgggttactg atttattgtt
     2581 gcggaaaaac gaggcgtaat cctgatattg caacattgcg atacccaacg cgcacaaaaa
     2641 ggaaacggcg gcaagcacaa ggcgcgtcaa aagctcctta taccaaacgc ggtatttaac
     2701 cttgacggcg atatacgcca gcgcgggcaa tacgcccaaa catacaatcc acagcacata
     2761 gcccggcgta atcaggcgcg cgctttcggc agccgtagtt tgcaagacat tattcaacat
     2821 cgacttgttg aaatagatat tgaaaaatat ttcttggtaa gacaccgccg cactgataac
     2881 caatatcaac ggaatcaata ccttatgcac gaaaggcagg gcaatgacgt gaaaaacgaa
     2941 attacttaaa aaaaacagca ccaccggcat cgtatagagg aagatatccg ccccggtgcc
     3001 gttaaaagga tgaagctcga caactttggc aaaaaaggcg taattcaata ccagcgagga
     3061 atacagggaa aggaaggcaa tcagcgcgga agagccgagc ttcggcctca ggttcggttt
     3121 tatcatttgg aatgtgtcgg ataagggttg gaaaaggcat ccggcatttg gaatccggat
     3181 tattgaaaaa gattcttaat tataaggcaa cggagcaaag cagggcaaga aaacggcggc
     3241 tgtgcggggg gttccgcccg ccattcaaac gtccggcaga cataaaaaca tcgtaagcaa
     3301 gattcgaacc ggtctgcaac cgcccctgcc agaaaaacgg gcaaagcatt tcatattgga
     3361 aaaacccggc cgcgccgccg acgggacagt ccggcacaaa cagcatcacg cccggattga
     3421 aaaggacgga tagccgccgg cagcacagtt ttaccccgca tcccaaaacg ccgccggaga
     3481 acgccgaaac agccgatgcc gtctgaagcc gcttcagaca acatcgggac atcaaccgta
     3541 acgccgttgg aaatcgcgca taaaatctgc caaagcccgc acgccttcaa gcggcatcgc
     3601 attataaatg ctggcacgca tcccaccgac agacttatag cccttcaaca ggcacaagcc
     3661 ctgcaattcg gcttccagca caaaacggcg gtcaagctcc tcatcccccg tttggaacac
     3721 gacattcatt ttagaacgcg cattcggacg gatacggttg atatagaaac cgccgctgcc
     3781 atctatcgtc tcatacaagg tttgcgcctt cagccgattg accgcttcaa tttttttcac
     3841 accgccctgc gcctgtagcc agcggaacac cagccccgac atataaatcg cgtaagttga
     3901 cggcgtgttg tacatacctt cgcggttcaa atgcgaacgg tagttgaaca catcgggaat
     3961 atcgttcgga caacgctcga gcaaatcctc acgcacaatc accaccgtaa cccctgccgg
     4021 cccgatgttt ttctgtgcgc ctgcgtaaat cagtccgtag tcggcaacat caaactcgcg
     4081 cgacaaaatc tcgctggaca tatcgcacac cagcggcggc atgccttctg aaaggcgcgg
     4141 cacttcacgg tattgcagcc cgttgaccgt ttcattgacg gcaaaatgga caaacgccga
     4201 atcgggtgca acatcccacg tttccacagg cggcaggtcg agatagtcga actgctcgcc
     4261 gccatgcgcc gccaaacgga tttccgtatc ggtcaaacgg ctcatctgtt cataagcgat
     4321 acggctccag ttacccgtta ccaccgcgtc ggcagtgcgg aaaccgtgtg ccagattcat
     4381 ggctgccata ttaaattggg ttgttgctcc accctgcaga aacaatatct tatagttgtc
     4441 aggcactttc aaaagctgcc tcaaatcctg ttccgcatga tgcaggatgc tcaaaaacat
     4501 ttccgaacgg tggctcatcg ccatcacagg aaaacccgta ccgttgtagt ccaacatttc
     4561 ctgccgcgcc gtttccaaca cggcttcggg caatacggca gggccggcgg aaaaattgta
     4621 aatcggataa agagacatga tgcagccttg attctgaaca ataacccgcc cgattttagg
     4681 cttgcagcag gctttgtgca aggatggaaa atacctgtcc tccgccccgc ttcatgccgc
     4741 ccgaacacag aaaacaacat caatatattg atttacaaac ataaaaatca tgcacgcgac
     4801 aaatagatac atttgttttg tcaacaatat tcacgatttc ccattacaaa cctcccttac
     4861 atccgctttt ttccgtccca aaaacacaaa ataaatcaac actttcattt ctccgcaaaa
     4921 gcggttataa tcacgccgat ttttcaactt tgacgaaaaa tggccacggg gccatttttt
     4981 tattatgtac atagggagca gcatggatat ccaaaccatc ctcgaaaaaa ccctgcccgg
     5041 cctgggctac gaactggtcg atttcgaact gaccgcgcaa ggaacattgc gcgtgttcat
     5101 cgacaaagaa agcggcatta ccgtcgaaga ctgcgcaacc gtcagcaacc acttgagccg
     5161 cgtcttcatg gttgaagaca tcgactacaa aaacctggaa atttccagcc ccggactcga
     5221 ccgccccttg aaaaaagccg ccgacttcgt gcgctttgcc ggtcagaatg ccaaaatcaa
     5281 aacccgcctg ccgatagacg gtcagaaaaa ctttatcggt aaaatcgaag gctgcgaaaa
     5341 cgataccgtt accgtatcct tcgacggcaa aaccgtacaa atcgaattgg gcaacatcga
     5401 caaagcccgt ctgcgccccg aattcaaatt ctaaaacaca acaatattgg agatgttcaa
     5461 aatgagtcgt gaaatgttac agctggcaga agcactggca agcgaaaaaa acgttgatgc
     5521 ggaagtcgtc ttccaagcac tggaattcgc cctgtctacc gccgccaaga aaaaggcaga
     5581 ccgcgaacac atggacgtgc gcgtccaaat caaccgcgac accggcgaat accaaacctt
     5641 ccgccgctgg ctgattgtcg ccgatgaaga ctatacctat cccgatgtcg aaaaaaccat
     5701 cgaggaaatc caagaggaaa ttcccggcac taccatccaa atcggcgaat actacgaaga
     5761 gcagctgccc aacgaaggct tcggccgcca agccgcgcaa accgccaaac aaatcatcct
     5821 gcaacgcatc cgcgatgccg agcgcgagca gaatctgaac gagtttctcg ccgtcaaaga
     5881 agacatcgtg tctggcacgg tcaaacgcgt cgaacgccac ggcatcatcg tcgaagtcgt
     5941 tgccggcaaa ctggacgcgc tgattccgcg cgaccaaatg attccgcgcg aaaacttccg
     6001 cagcggcgac cgcatccgcg ccctcttcct gcgcgtcgaa gaaatcggca acaccggccg
     6061 caaacaagtc atcctgagcc gtacttccgg cgatttcctc gtcaaactgt acgccaatga
     6121 agtacctgaa attgcagacg gcatgcttga aatccgcgct gtcgcccgcg acccgggcca
     6181 gcgtgccaaa gtcgccgtca aagccaacga ccagcgcatc gatccgcaag gcacctgtat
     6241 cggcgttcgc ggttcgcgtg tcaatgccgt cagcaacgaa ttgtccggcg agcgcatcga
     6301 cgttgtcctt tggtcgcccg agcctgccca attcgtcatg agcgcgctct cacccgccga
     6361 agtcagccgc atcgtcatcg acgaagacaa acacgccgtc gatgtcatcg ttgccgaaga
     6421 ccagctcgcg ctcgccatcg gacgcggcgg tcaaaacgtg cgccttgctt ccgacctgac
     6481 cggctggcag ctcaacatca tgacttccgc cgaggcagac gaacgcaatg cggcagaaga
     6541 tgccgccatc cgccgcctgt ttatggatta cttgaacgtg gacgaagaaa ccgccgacgt
     6601 actggttcag gaaggttttg caaccttgga agaagtcgcc tatgttcctg ccgccgaact
     6661 gcttgccatt gaaggatttg acgaagaaat cgtcgatatg ctccgcaacc gcgcccgcga
     6721 tgccatcctg accatggcga ttgccgccga agaaaaactg ggcgaagtgt ccgacgatat
     6781 gcgcaacctc gaaggcatag atgccgatat gctccgcagc cttgccgaag caggcattac
     6841 tacccgcgac gacttggcag aactgtccgt ggacgaactg attgaaatca ccggtgtaaa
     6901 cgaagaaacc gcaaaagccg tcatcctgac cgctcgcgaa cactggttta ccgaagacaa
     6961 ataaaagggg gtacagatga gtaacacaac cgtagaacaa tttgccgccg agctgaaacg
     7021 ccccgtcgaa gacctgttga aacagttgaa agaagccggc gtcagcaaaa acagcggcag
     7081 cgattccctg acgctggacg acaaacagct tctgaacgcc tacctgacca agaaaaacgg
     7141 cagcaacggc ggcaccatca gcatccgccg caccaaaacc gaagtcagca ccgttgacgg
     7201 cgtaaaagtc gaaacacgca aacgcggacg cactgtcaac attccttctg ccgaagaatt
     7261 ggcagcacag gtaaaagccg cccaaaccca agccgcacct gtccggccgg agcagacggc
     7321 agaagacgcg gcaaaagccc gagccgaagc tgccgcacgt gcagaagccc gtgccaaggc
     7381 agaagcggaa gcggcaaaac tgaaagcggc aaaagcaggc aacaaagcca aacctgccgc
     7441 gcagaaaccc accgaagcaa aagccgaaac cgcacccgtt gcggcggaaa ccaaacccgc
     7501 cgaagaaagc aaagcggaaa aagcccaagc cgacaaaatg ccgtctaaaa aacccgccga
     7561 gcccaaagaa aaagccgcca agccgaaaca cgagcgaaac ggcaaaggca aagatgccaa
     7621 aaaaccggcg aaacctgccg cacctgccgt gccgcaaccc gtggtcagcg cggaagaaca
     7681 ggcgcaacgc gatgaagaag cacgccgtgc cgccgcactt cgcgcccacc aggaagccct
     7741 gttgaaagag aaacaggaac gccaggcacg ccgcgaagcc atgaaacaac aggcagaaca
     7801 acaggcaaaa gccgcacagg aagccaaaaa cggcagacag cgtcccgcca aacctgccga
     7861 gaaaccgcag gcagccgcgc cggccgtcga aaacaaacct gtcaatccgg caaaagcgaa
     7921 aaaagaagac cgccgcaacc gcgatgacga aggtcaaggc cgcaacgcca aaggcaaagg
     7981 cggaaaaggc ggacgcgacc gcaacaatgc acgcaatggc gacgacgagc gcgtacgcgg
     8041 cggcaaaaaa ggcaaaaaac tcaaactcga gccgaaccaa cacgccttcc aagcaccgac
     8101 cgaacctgtc gtacacgaag ttttggtgcc tgaaaccatt accgttgccg acttggcaca
     8161 caaaatggca gtcaaaggcg tggaagtggt caaagccctg atgaagatgg gcatgatggt
     8221 taccatcaac caatccatcg accaagacac cgccctgatt gtcgtcgaag aactcggaca
     8281 catcggcaaa cccgccgcag cggacgatcc cgaagccttc ttggacgagg gcgcagaagc
     8341 agtggaagcc gaagcattgc cgcgtccgcc cgttgttacc gtcatgggac acgtcgacca
     8401 cggcaaaacc tcgctgctgg actacatccg ccgcgccaaa gtggtacagg gcgaagcggg
     8461 cggcattacg cagcacatcg gcgcgtacca cgtcaaaacg ccgcgcggcg tgattacctt
     8521 cttggacacc ccgggtcacg aagcctttac cgctatgcgc gcacgcggtg cgaaagcaac
     8581 cgacatcgtg attcttatgg tcgccgccga cgacggcgtg atgccgcaaa ccatcgaagc
     8641 gattgcccac gccaaagcgg caggcgtacc gatggtggtt gccgtcaaca aaatcgataa
     8701 agaagccgcc aacccagagc gtatccgcca agagctgacc gcacacgaag tcgtgcctga
     8761 cgaatggggc ggcgatgtac agtttatcga cgtttccgct aaaaaaggcc tgaacatcga
     8821 tgcattgctc gaggcagtct tgctcgaagc cgaagttttg gaactgaccg cacctgtcga
     8881 tgcgcccgcc aaaggcatca tcgtcgaggc gcgcttggac aaaggccgcg gcgcggttgc
     8941 cacattgctg gttcaaagcg gtacgctgaa aaaaggcgac atgctgctgg ccggtacggc
     9001 attcggcaaa atccgcgcga tggtcgatga aaacggcaaa tccattaccg aagccggccc
     9061 gtccatcccg gtcgaaatcc tcggcttgtc cgacgtaccc aatgcgggcg aagacgcgat
     9121 ggtattggcg gacgagaaaa aagcgcgcga aatcgccctc ttccgccaag gcaaataccg
     9181 cgacgtgcgc cttgccaaac agcaggcggc gaagctggaa aatatgttca acaatatggg
     9241 cgaaacccag gcccaatctt tgtcggtcat catcaaggca gacgtgcagg gctcttacga
     9301 ggctttggcg ggcagcctga aaaaactgtc cacagacgaa gtgaaagtga acgtgttgca
     9361 cagcggcgtg ggcggcatta ccgaatcgga tgtcaacctt gccatcgctt cgggcgcatt
     9421 cattatcggc tttaacgtgc gtgcagatgc ctcttcgcgc aaacttgccg aaaatgaaaa
     9481 cgtggaaatc cgctactaca acatcatcta cgatgccatc gacgacgtga aggcggcgat
     9541 gagcggtatg ctttccccgg aagagaaaga acaggttacc ggtacggtcg aaatccgtca
     9601 ggtcatctcc gtttccaaag tcggcaacat tgcaggctgt atggttaccg acggcgtggt
     9661 caaacgcgat tcccatgtcc gcctcatccg caacaacgtg gttatccaca cgggcgaact
     9721 ggcttcgttg aaacgctata aagacgatgt aaaagaagtc cgcatgggct tcgagtgcgg
     9781 tctgatgctc aaaggctaca acgaaatcat ggaaggcgac caactggaat gcttcgacat
     9841 cgtcgaagtt gcccgcagcc tgtaattcct ttgcaaataa aatgccgtct gaagcgttca
     9901 gacggcaacg aaacgggttc tgtatcatac agaacccgtt ttttgtcgca aatcggcttc
     9961 agacagccct cttgccttat cccgatttga atctgacttg ccatacaaac aggcttcaga
    10021 cggcattata gtgaattaaa tttaaaccgg tacagcgttg gctcgccttg ccgtactatt
    10081 tgtactgtct gcggctcgcc gccttgtcct gatttaaatt taattcacta tattttcccg
    10141 acaggcggat ttgaagtttc tccacatatc cggcgcgttc ggcgcggctg gtttcggggc
    10201 ggtgcgtatt gagcgacgac catttccaat gactgcgggc tttgttgagt tcgggcggga
    10261 gtctggcggc gtcccacggg gctttgcggc tgtgcagctc gatatccgac tgtgccgcgt
    10321 gtccgcgcgt ttgcaggacg tggagcaaat cgagggcgcg ggcggcgagc agggtcaggg
    10381 tttcagggtc ggtgtgcagg gtttggcggc cagcgagttt gtcggaaatg gtgcgggtat
    10441 tgggcaggat gccgcccaaa aagccgccga ttgccgtacc caatccgagc gagccgccga
    10501 gtgtggcgat gtccagcccc aagccgatga gcgcgccggt tgccgcgccc gtgccggtgc
    10561 ggatgccgta ttgtttgagc aattcgctgt cgaacgggtc ttggcggaag gcttgcggca
    10621 tccagtcgcc gccgtcgatt tcgctgtggt agaaacggta gagggcaaac agccgctgct
    10681 gcatctgccg ttcgagttgg cgtatttccg cctgcatggt ttgcagcacg gtggcggtat
    10741 cctcgttttc gtccacttcc tgcctgaagg cggcggcatc aattaaaaag tcggcgattt
    10801 cgcggcgcgc ttcgccgtcc agccgctgcc attcgcgccg gcgcatggct gtcaggcggt
    10861 caagtgtgct gcgttcgggc aacatggtgg cgaggttttc ccacaggcgt agttcgcctt
    10921 caaaatcaaa ggcgacggtg tcgaaccctg cgaaaacgtg caggtttctc ctcgccagca
    10981 tggttgtcca cgattcggga ggctgtccgc cggtaaagtt gaacacgggc ataaccggtt
    11041 tggcacacca tgaaaggatg gtcaactcgt ccctgtattt gtcgaggacg ggttcgcgcg
    11101 cgtcgatgac gtacattgcc atatcgcttt gcaagacttg ccgtaagact ttggcttcct
    11161 gattgaaatc atggtgcgcg ccgtggctgc cgagaaactg ttgcagccgt tcgatgccgt
    11221 ctgaacgatt gtccgtatgg ttttccagcc attccagcac gccgcccgcg tcttcgagtc
    11281 cgggcgtgtc gtacaggaaa accagcgtgt ctgcgccgtc gctgatggcg gcttcttcga
    11341 catgacgcgt ggtcgatggg gcgtttttga cttcgccgaa accgctgtcg cgcaaaaggg
    11401 tacgcaggag cgaggttttg ccggtgttgg tgtgtccgac gacggcgagg gaaaggggtt
    11461 gtttgttcat gatgtttttg aagaatggat tttcagacgg tcttttgtca gaatggcggc
    11521 ttaacagaac atttcaagtg agtttattgg tctttcaaac gcccttcctg cgccgccctg
    11581 tcaggctcaa gccacgccgc gccgcattcg gccagcgcgt tacgccaatg ttccagcttt
    11641 tccgaaaggt cgtctgaaag cccctgttcc gccaaaagct gcaccaccgc gccgccctgc
    11701 gccgcttccg aaagtcggac gatctgccgc aacacgccgc ggtcgggcac agtttgggcg
    11761 cgcacgccga taagcagttg cgccggtttc tgcttcagct ctgtctccag cgcggcaacc
    11821 tgttcccgat tggtggcaac gcccttatcc agccattcct gcgccagcct gccctcgaac
    11881 cattcgccgt cctgccactc ggtctccagc atgaccgccc atttcggcgc atcgttcaag
    11941 acgattttcg gcgaaacggc ggacacggtt tcccgacgcg tatccgcatc ggtaattttg
    12001 ttctgccagc ggcggatgac cgcctgataa tagggttttt ccaaatccaa gccgtttttg
    12061 cttgttttca aaaggatttt acacaccacc caagccaaga agcgcggcag gatgccgtag
    12121 caggcgatac tgccgaccag cagccccgac caagcctgcg catcggcaat attgccgttc
    12181 agacgacctt caactaccgc ccgcgcatcg gggacaggga aaccgagttt cgacggcagc
    12241 catgccaaca tttccaccag ccgtaccgaa gcggcattgc tcaacagcgt gctttcccag
    12301 ttgaacgtat attggcgcac caaaagcagc aacaataccg acaccagcat tccgagcagc
    12361 gtgcagagcc acaggctgtg cgacgttgcg cctattttcc aacgtaccga aggttgccgc
    12421 cactcgtccg catacagccg caacaccgcc tgatttacag ggtctttgcc ccgaaaccac
    12481 gtcgccggac tgctgaaaaa acgccccact ttcacgcgca ggaacaacat tgccaaccat
    12541 actgccagca tcagcgtatt catgcccaac acgcccgcca aaaccaaaaa gaaattcaga
    12601 ccctgattgt ccattagaag ataagtacct gaaaaaccgg taaaaaacgc aaacgtcgcc
    12661 gccgccaccc acaaccagaa cgaccccgca cgcacacgtt ccaatgtgtc ccgcagcata
    12721 cggttcctgt cgatcatctc cgcccgacgg atgatttttt cctccgtact gccgtccacg
    12781 cggcgcaaag cctccgtcgc ctgtacggga tcgccgctga aaataaaacc gccttcgtcc
    12841 aaaatacgga ccagctcaac cagttttcgg gatggattca acataaaatg ccgtctgaaa
    12901 ataaaaaaca gattttaaca cacgcatttt caagaatatt cacagtgtag gcaaagagta
    12961 aatcccacac agaggcaaaa gtatcggcgt aaactgactg cctctacttt cccgaaagat
    13021 tgtgcgatgt atacaggcga acgcttcaat acttacagcc atttgagcgg tttgattctg
    13081 gcggcggcag gtttggcgct gatgctgctg aaaaccatag gacacgggga cggctaccgt
    13141 atcttcagcg tatcggttta cggcatcagc cttcttctgc tctatttgag ttcctcgctg
    13201 taccacggaa ttgcagccgg aaaactgaaa agcattttga aaaaaaccga ccactgcatg
    13261 atttatgtgc tgattgccgg aagctacaca ccgtttgcac tgatttcttt gagaaacggg
    13321 ccgggctgga cggtattttc actgtcctgg ctgctggcgg ctgcaggaat cgcacaagaa
    13381 ctcaccatcg gacggaaaag cgaaaaacgt ctgctgtcta ttgtgattta tgtcgtcatg
    13441 ggttggatgg tcttggcggt aatgaaatcc ctgacagcct cactcccgtc ggcaggactg
    13501 gcttggctgg cggcaggcgg tatgctgtac agcgtcggca tttactggtt tgtaaacgat
    13561 gaaaaaatcc gacacgggca cggaatctgg catctgttcg tattgggcgg cagcatcacc
    13621 caatttgtca gcgtgtacgg ttacgtaatc tgaatgccgt ctgaaaagca aaacctcccg
    13681 atcctgaaga ttgggaggtt ttctgtttgc cggacatcag cccttgtcgt ggaactcgtg
    13741 gaattcatac ggataggaca aatcccgacc cgcttttttc tgtgccaaat aatcatcata
    13801 aatggcacgg atttccttac gcaacaaaaa cagggctatc aagttcggga taaccataaa
    13861 gccgttgaac atatcggaca ggctccaaac caaatcgact ttgccgagcg tacctaaaac
    13921 gatggcaagc agaaccaatg cgcgatagat gcccaagtgt cttcccctga aaagaaaacg
    13981 gatgttggac tcgccgaaat aataccagcc gataatggtc gtcaaggcga aaaaggtcag
    14041 acacacggca agcaattgcg aaccgaagcc cggaaatgcc ttgttaaagg caaattgagt
    14101 aaccgccgcg ccctgttcgc ccgaaaggtt ggcatcggtc agcaggataa tcaatgccgt
    14161 agccgtacat accaaaatcg tatcgataaa tacaccgaca aatgccgcca taccttgctg
    14221 cacaggatgc ttcacatccg cagtcgcgtg ggcgtgcgga gtcgaaccca tacctgcttc
    14281 gttggaaaac agaccgcgcg ccacgccgaa acgtatcgct tcgcgcatac cgatacccgc
    14341 agcaccgccc aaaacggctt cgggattgaa ggcggcggta aagatgtggt tgaacatcgg
    14401 cacaatatgg tcggaaaatt caaacaggat aacgacggcg cacaaaatat aaacaaccgc
    14461 cataaacggc acgacaaatt gggcaatatt ggcaatacgg ttcacgccgc caatcacaac
    14521 catgcccgca aggacggcaa gcacaatacc gactgccaaa gaaggcacat caaatgcaat
    14581 ggtaacggca gaagcgatgg agtttgcctg agtcgcattg ccgataaagc ccaacgcgac
    14641 aatcagcgcg atggagaaaa agcccgacag gaaacgcgcc gcgcccctgc cgattttcgg
    14701 ggtcagaccg tgcgtaatat aaaacgcggg gccgccgatg tatttgccgt ggctgacgac
    14761 gcggtatttc tgcgccagca gtgcctccgc aaaaatcgtg gacatcccca aaacggcaga
    14821 aagccacatc caaaaaatcg cgcccggccc gcctgcggta atggcggtcg acacaccggc
    14881 gacgttgccc gtaccgattt gcgcggatat agcaaccgcc aacgcctgaa actgcgataa
    14941 agacttatcg tctttattgc ctttggcaaa caagccgcca aatacggatt tgaatcccgc
    15001 gcccagcttg gtaatctgcg gcgcaccgag atacagcgta aaaaacaggc cgatacccaa
    15061 aagcgcgtaa atcagcaggt agtcccaaag gaaccgattg actgtaccca ccagaacaga
    15121 caatatattt tccataaaat aaaccttatc ttacgattaa aatgactgcc ttccaaaaga
    15181 cattccaata aggaaacacg gcgagcagac cgtatttgcc gcaacagatg ccttaaattg
    15241 tcaacaatcg gggagaagct gcgccatcat accgtaaaat atcgttaatt aaaacatttc
    15301 tttattttta agcggaaagc ggaggaaatc gctttcagac ggcatcgaca acggcacggc
    15361 ataaaacagg atattttggg tacttgcaac ttatgttaaa atgccgaccg taaaaaatct
    15421 gacaaaaaca gattaattat ttgaaataag aaaggaaatt tatggcaggc catagcaagt
    15481 gggcgaatat ccagcataaa aaagcccgtc aggatgccaa acgcggcaaa atcttcaccc
    15541 gtttaatcaa agaaatcacc gttgcggcgc gtatgggcgg cggcgatccc ggcgcaaatc
    15601 cgcgcctgcg cctggctttg gaaaaagcag ccgaaaacaa tatgcccaaa gacaatgtgc
    15661 aacgcgccat cgacaaaggc acgggcaact tggaaggcgt ggaatacatc gagttgcgct
    15721 acgaaggcta cggcatcggc ggcgcggctt tgatggtgga ctgcctgacc gacaacaaaa
    15781 cccgcaccgt tgcggacgta cgccacgcgt ttaccaaaaa cggcggcaac ttgggtaccg
    15841 acggctgcgt ggcgttcaac ttcgtgcatc agggctattt ggtattcgaa cccggcgttg
    15901 acgaagacgc gctgatggaa gcggctttgg aagccggtgc ggaagacgtg gttgccaacg
    15961 acgacggttc catcgaagtc attaccgcgc caaacgattg ggcgggcgta aaatccgctt
    16021 tggaggcggc aggttacaaa tccgttgacg gcgacgtaac catgcgcgcc caaaacgaaa
    16081 ccgaactctc cggcgacgat gccgtcaaaa tgcaaaaact gattgacgcg ctggaagact
    16141 tggacgacgt gcaagacgtt tacacttccg ccgtattgaa tttggactga tacgcagcac
    16201 agcagacata caataaaatg ccgtctgaac ctttcagacg gcattgattt atttagccct
    16261 tgcccacgcc caccaaaccg tcaggacaac cgccagaaaa tacgccacgc tccaaacagg
    16321 caaggcaatt cccagcagat aatccggttc ggcacaattt ccgaacccgc gcacgacagg
    16381 ctcgaaccaa tcaaacaaag gccagccttt caagcggaac gtccacggcg cgccgcacga
    16441 aggagcggtt cccggcggca gcgactgcaa ccacaactga tatgccgcaa cagaaatacc
    16501 cgtaacggcg ggaatgctga taaagacagc accgaacaaa ccgcctgcct ttcttcttgg
    16561 tctgcacatc aggacaattg ccgcacacaa tgcggttgcc aagacgcaca accgctggct
    16621 gatacacaaa acgcaaggct ccatacccaa aacatactgt gccgccaaag agccggcaaa
    16681 tgcacagacc gaaacggcaa acagcagcca aacggctttt ctaaataacg gggtcatttt
    16741 ctcaacacac caatcaaaat accgatatgc cgattttgct ggatatatcc cgagatggca
    16801 agggacgaat cgacaccggc gcaggtgcgg acgatttcgg ttttcccgat atacacaaga
    16861 cctgacaagg gacaaaacgg cgatttgccc gcacaatgcc cgcaaaaaaa tacatccgga
    16921 ggatttgaat ttcggcaaat ttagcgaatc aaaagtttat ttcaatgaaa tcatatgatt
    16981 tttttgaata agcggattga caggtttttg aaggaatttg ttaccggata gccatcgggc
    17041 aagttttttg caaaatttga atcggtcggg taaattttca aaaaataatt gacagcggat
    17101 aagaaacggc ggataattcc cgccgtcgag ttgcttgatg cagcttgatt tttctcctct
    17161 atttctcctt tgtagacttg gcacacattc aactggatgt gtgcattttt ttatctccgc
    17221 tttttttgaa atttaattac ttttattgtt tgaaattcca ttctttaaga aatttaacaa
    17281 gaaaaacgct tgctttttga ccggaaaagc tgcataattc attccgttag ctggttcgag
    17341 tagtcagtta atagtttctc ctctatttct cctttgtaga cttggcacac attcaaccgg
    17401 atgtgtgcat ttttttatct gaagcaacaa gcctctgtgc gtgatgttgt tatgtttcat
    17461 ttaggtgtca aaccgcatag ccagcctgaa atattcaatc caaatccaaa actggatttt
    17521 ctttgacctc ctccatcaca acataactcc tactctccga agcggcaggc agttgcaata
    17581 ggatattgcc tagcatatcc cgataggcag acatatcggg caaacgtact ttaatcagat
    17641 agtcgtattc gcccgacacc aagtggcatt ccataatttg cggaattttc agcacttctt
    17701 ttttgaaatc ttcgaaaata ttgcccgatt tggattgcag cttcagctcg acaaaaacca
    17761 atagtggttt gcccaacaga tggggattga gatgggcgtg atagccggaa atataatgtt
    17821 cccgctccaa acggcgcacc ctctctgtaa cgggcgtggt ggacaagcct accttctcgg
    17881 caagctccat catcgggatg cgggcattct gttggaggat cttaagaatg cggaaatcga
    17941 ttttatctag ttctttcatt tggattgcct tgtatttatt attgatttta acaaataga
//
LOCUS       DAXHRI010000027        17772 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXHRI010000027 DAXHRI010000000
VERSION     DAXHRI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.027"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(59..1339)
                     /locus_tag="ACFM75_000691"
     CDS             complement(59..1339)
                     /locus_tag="ACFM75_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC6255274.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            complement(1561..2181)
                     /gene="tmk"
                     /locus_tag="ACFM75_000692"
     CDS             complement(1561..2181)
                     /gene="tmk"
                     /locus_tag="ACFM75_000692"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC6255275.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGETLREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRNLDEVRNSIEKVLDGHFGC"
     gene            complement(2241..3236)
                     /gene="mltG"
                     /locus_tag="ACFM75_000693"
     CDS             complement(2241..3236)
                     /gene="mltG"
                     /locus_tag="ACFM75_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC6255276.1"
                     /translation="MLKKMLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3320..3892
                     /gene="ampD"
                     /locus_tag="ACFM75_000694"
     CDS             3320..3892
                     /gene="ampD"
                     /locus_tag="ACFM75_000694"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC6255277.1"
                     /translation="MDNHAEAHWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISL
                     PPFEYGTDAVEKLFANRLDPNDHPFFGLIHTLRVSSHFLIKRDGKTVQFVSCGDMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4086..5372
                     /locus_tag="ACFM75_000695"
     CDS             4086..5372
                     /locus_tag="ACFM75_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC6255278.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5520..8045
                     /gene="ligA"
                     /locus_tag="ACFM75_000696"
     CDS             5520..8045
                     /gene="ligA"
                     /locus_tag="ACFM75_000696"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC6255279.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNVKTVANIPLRLHGENTPELIEVRGEVLMQKADFATLNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QELGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKEAADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8102..9277
                     /gene="hemW"
                     /locus_tag="ACFM75_000697"
     CDS             8102..9277
                     /gene="hemW"
                     /locus_tag="ACFM75_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC6255280.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALENAGFIHYETSAFAKPAMRCRHNLNCWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQNRPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETNPAVFRPTERGRLFLNDLLQCFL"
     gene            9768..10295
                     /gene="nspA"
                     /locus_tag="ACFM75_000698"
     CDS             9768..10295
                     /gene="nspA"
                     /locus_tag="ACFM75_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC6255281.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQVPSTDFKLYSIGASAIYDFDTQSPVKPYL
                     GARLSLNRASANLGGSDSFSQISTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNNVK
                     NVRTGELSAGVRVKF"
     gene            10432..10821
                     /locus_tag="ACFM75_000699"
     CDS             10432..10821
                     /locus_tag="ACFM75_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6255282.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARRVFQNLQAVAEAADGSLDDIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            10899..11729
                     /locus_tag="ACFM75_000700"
     CDS             10899..11729
                     /locus_tag="ACFM75_000700"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC6255283.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGSK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            11744..13201
                     /locus_tag="ACFM75_000701"
     CDS             11744..13201
                     /locus_tag="ACFM75_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC6255284.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMIVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(13242..13631)
                     /locus_tag="ACFM75_000702"
     CDS             complement(13242..13631)
                     /locus_tag="ACFM75_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255285.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(13813..14214)
                     /locus_tag="ACFM75_000703"
     CDS             complement(13813..14214)
                     /locus_tag="ACFM75_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255286.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(14245..14595)
                     /locus_tag="ACFM75_000704"
     CDS             complement(14245..14595)
                     /locus_tag="ACFM75_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255287.1"
                     /translation="MPSETVFNFQATFPLFETGYWELSSSKILHLLLPRQHLGQNGRR
                     LKSKHRPSCCRLRMSTGQPQCRRNRRSRLQHCRRQSRKVGKNGKTGEIKADGRKVNVR
                     IDSTEADLLYPAGQ"
     gene            complement(14614..14991)
                     /locus_tag="ACFM75_000705"
     CDS             complement(14614..14991)
                     /locus_tag="ACFM75_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6255288.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGISTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(15022..15420)
                     /locus_tag="ACFM75_000706"
     CDS             complement(15022..15420)
                     /locus_tag="ACFM75_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6255289.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
     gene            complement(15426..16103)
                     /locus_tag="ACFM75_000707"
     CDS             complement(15426..16103)
                     /locus_tag="ACFM75_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HFC6255290.1"
                     /translation="MVKTADGYKAIARIRTGDRVFSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNNQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGSQAETEGVWVHNACPPRKTPSTPVYGNDSEAYAAAK
                     KLGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKETRNGTFDK
                     NLNRIGD"
     gene            complement(16237..16554)
                     /locus_tag="ACFM75_000708"
     CDS             complement(16237..16554)
                     /locus_tag="ACFM75_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255291.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(16557..>17772)
                     /gene="mafB"
                     /locus_tag="ACFM75_000709"
     CDS             complement(16557..>17772)
                     /gene="mafB"
                     /locus_tag="ACFM75_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6255292.1"
                     /translation="AAFGQPALAADLAQDPFITDNAQRQHYEPGGKYHLFGDPRGSVS
                     DRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHGHEEHAPFDNHAADSASKE
                     QGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGARDEYTYHVNGTARSIKLN
                     PTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFINGVAAGAL
                     NPFISAGEAVDQWMQENPNAAETVETLVNVLPFAKVKNLTKAAKPGKAAVSGDFSDSY
                     KHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKSTLNQN
                     INPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKNGVHI
                     VPANPKTIKKVQ"
BASE COUNT         4202 a         5107 c         4203 g         4260 t
ORIGIN      
        1 tctgacagca aaatgccgcc tgaaaacagt tcagacggca ttttaaaccg caaacggctt
       61 acagcttcca ttcgcccagc ttggcggcgt aggcttcaaa gtcggcaatc ggacgggttg
      121 caacgccgct ttccatcgct gctttggcgg cagccgtagc aacgcgcggc agcaggcggg
      181 aatcgaacgg agtcggaatc aggtattccg cgccgaattc aaatttctta ccgtaagcgg
      241 caaccacttc ttcggttact tcttccatcg ccaaatccgc caaagcatac acgcaggcgc
      301 gtttcatttc ttcgttgatg gtggtcgcgc cgacatccaa cgcaccgcgg aagatgaacg
      361 ggaagcacaa tacgttgttc acttggttcg ggaagtctga acgtccggta ccgataacca
      421 cgtccggacg ggtttctttc gccagcggcg gcaggatttc cggattcggg ttggccatgg
      481 cgaacacgat gggtttttcg ttcatggtgt tcagcatttc aggcgtcagc aggttcgcgc
      541 cggagaggcc caagaagatg tctttgcctt tgacggcatc ggcaagcacg cgttggccgt
      601 tgtcttcaat ggcgtagaac tgtttagact catccatgcg gtctttgtct tcgcgggttt
      661 ggtaaatcac gcctttggag tcgcaaacgg tcacgttttc gcgtttcaag cccaaatcca
      721 gcaattggtt caagcaggca atcgccgccg cacccgcgcc ggagcaaacc aaggtcgctt
      781 cttcgatttt acggccggta aaacgcaggg cgttcaatac ggcggcggcg gtaatgatgg
      841 cggtaccgtg ctggtcgtcg tggaatacgg ggattttgca gcgtttgcgt aattcgcgtt
      901 cgatgtagaa acactcgggt gctttgatgt cttcgaggtt gatgccgccg aaagtcggct
      961 ctaaagcggc gatgatgtcc accagttttt gtgggtcttt ttcatcgatt tcgatgtcga
     1021 acacgtccac accggcgaat tttttgaaca atacgccttt gccttccatc acgggtttgc
     1081 ccgccagcgc gccgatgtcg cccaagccca aaacggcggt accgttggaa atgacggcga
     1141 ccaagttgcc tttggcagtg tatttgtagg cattttgcgg atcggcatgg atttccatac
     1201 aaggagcggc tacgcccgga gagtacgcca acgccaggtc tttgtcggtc gccagagatt
     1261 tggtcggggt aacggaaatt ttgcccggaa cgggtaattc gtggaacttg agggcggctt
     1321 ctttcaatga gttttccatg tgtcaatcct gttgcgagaa aaagatttga atcggactgt
     1381 ccgaaacgct ttcgggtcat catgttacgg gcggttcgtt aatattgccg acgcatcctg
     1441 ccttgtaacc gagcgtaatt ctatcatgcc gaaagtttgg cgcataccgc cattttcacc
     1501 tgcggcactg ccgtttgatt ttcggcgcgg gcggatgcgc ttatttcaat atttgccgca
     1561 tcagcagccg aaatgtccgt ccaacacttt ttctatgctg tttctgactt catccaagtt
     1621 gcggttactg tcgataacgg cgtaccgttc gggacaggcg gcggctcggt cgagataaac
     1681 gccgcgcaca cgcataaaga aatccgcctg ctcctgctcg aaacggtctt tctcgcgcgt
     1741 ctgcccgata cgcgccatcg acacttcgag tggcacatcc aacagcaggg tcaaatcggg
     1801 gcgcaaaccg ccctgcaccc aatgttccaa aatttcaatg tcttcagacg gcattccccg
     1861 cccgccgccc tgataggcga aggttgcgtc ggtaaaacgg tcggacacga cgtgtatgcc
     1921 gtctgaaagc gcgggcaata tcacgtcttc aatatgctgc attctggcgg cgaacatcat
     1981 cagggtttcc gcacgcaaac cggctttggt ttcagggttg agcaggattt cgcgcaaggt
     2041 ctcaccgacc ggcgttccgc ccggctcgcg cgtgaacagc acgggcagcc ccctccgttc
     2101 aaaccatgcc ttgatgacgg caaggttggt ggatttgccg gcaccgtcta tgccgtccaa
     2161 agtgatgaat tgcggtttca tgcttgcagt tccggtaagg gtatgccgtc tgaaaacaca
     2221 aacttttcag acggcatggt ttattttttc aaaatatatt tgcggacggc ggcattgtgt
     2281 tcggtcaaat catggctgaa ctggctcaag cccgtgccgt ccattttgga cacgaaatac
     2341 aggtattttt cgccggacgg atgggcggcg gcatcaagcg cggccttgcc gggcagcgca
     2401 atcggggttg gcggcagacc gccgcgcgtg taggtgttgt acggcgtgtc gcggcgcagg
     2461 tcggctttac ggattttgcc cttgtatgcc gcacccatgc cgtaaatcac ggacgggtcg
     2521 gtttgcagac gcataccgat tttcagccgg ttgacgaaga cggaagcgac atggtcgcgg
     2581 tcggcttcat gccccgtttc cttttcgacc aggctcgcca taatcagcat ttcataagga
     2641 tttttataag gcagcccgtc ctgcctgctt tcccatgcct cattcaggcg gcgttgcatc
     2701 gccttgtagg cggtttggta aatctgtaaa tcgctgccgc ccgcatcgat ttcgtagctg
     2761 tcggggaaaa actgcccttc aggattgccg ctgaaggcat caggggcaac ttccgccatc
     2821 agtttttcat tgctccagcc tttggtgtcg tgttcgatgt cgggcgttgc gtcgatgact
     2881 ttcctcatat gcgaaaaacg cgaaccttcg ataatctgca cggtaacgga atccggcctg
     2941 ccgccgcgca ttttctgcaa gatatcccaa gcagacactt ccgaaggcag tctgtacgtc
     3001 cccgtatgca gcctgttgtg cacacccaaa acgtaggccg ccgccgtcaa aacatgcctg
     3061 ctgaacacga tgcggtcttc ggcaagtttc ctgccgaccg acgaaatacc ctggtttttg
     3121 gcaattttga ttcggtatgc cctgccgtta tccttaggaa caaaaagcag cgcggcgaaa
     3181 acggctgccg acacggtcaa aaaaacggca gaccatttca acattttttt caacatggta
     3241 ggattcccag cattcggaat acgtcttaaa attcgggcga cagtataacc caaattgccc
     3301 gccggacagg aagaacatca tggacaatca cgccgaagca cactggcagg acggttggct
     3361 gcaaagcgtg cgccacattc cgtcgccgaa tttcggcccg agggagtcca aagaagaggt
     3421 ttcccttgtc gtattgcaca atatctcgct gccgccgttc gaatacggca cggatgcggt
     3481 ggaaaagctt tttgccaacc gcctagaccc gaacgaccac ccgtttttcg gattgataca
     3541 cactttgcgc gtatccagcc atttcttaat caaacgcgac ggcaaaacgg tgcagttcgt
     3601 atcatgcggc gatatggcgt accacgcggg cgtatcctcg tttcgcggac gggaaaaatg
     3661 caacgcattt tccatcggca tcgaactgga aggctgcgat ttcgaaccgt ttgccgaagc
     3721 gcaataccgt tcgctcgaag ccttgttgga cgcaatctgc cgccgctacc ccatcacagc
     3781 ggtaacgggg caccaagaca tcgcgcccgg ccgcaaaacc gaccccggcc attttttcga
     3841 ctggcggcgg atacggggga aagggtttcc cgtggacaga aatgccgtct gaaaaccaca
     3901 gatttcagac ggcatttcgg aaaatttccg cttatttcga cacactgctg tcagaccctg
     3961 caattttcgt tataattcaa acgttataac cggccgttcc atcagactgc cggaaacgca
     4021 agcccgattt aaccgcagta cccgcacttc gcgcatcctg catcccgaaa tttaaagtga
     4081 ttgccatgat ttacatcgta ctgttcctcg ccgccgtcct cgccgttgtc gcctacaata
     4141 tgtatcagga aaaccaatac cgcaaaaaag tgcgcgacca gttcggacac tccgacaaag
     4201 atgccctgct caacagcaaa accagccatg tccgcgacgg caaaccgtcc ggcgggtcag
     4261 tcatgatgcc gaaaccccaa ccggcggtca aaaaaacggc aaaaccccaa gaccccgcca
     4321 tgcgcaacct gcaagaacag gatgccgtct acatcgccaa gcagaaacag gcaaaagcat
     4381 caccgttcaa aaccgaaatc gaaaccgcct tggaagaaag cggcattatc ggcaactccg
     4441 cccacaccgt ttccgaaccc caaaccggac attccgcacc gaaacctgcc gacgcgccgg
     4501 caaaacctgc acccgttccg caaacacctg caaaaccgct gattacgctc aaagaactgt
     4561 caaaagtcga attaccctgg tttgacgtgc gcttcgactt catctcctat atcgcgctga
     4621 ccgaagccaa agaactgcac gcactgccgc gcctttccaa ccgctgccgc taccagattg
     4681 tcggctgcac catggacgac catttccaga ttgccgaacc catcccgggc atccgctatc
     4741 aggcatttat cgtgggtatt caggcagtca gccgcaacgg acttgcctcg caggaagaac
     4801 tctccgcatt caaccgccag gtggacgcat tcgcacaaag catgggcggt cagacgctgc
     4861 acaccgacct tgccgccttt atcgaagtgg cttccgcact ggacgcattc tgcgcgcgcg
     4921 tcgaccagac catcgccatc catttggttt ccccgaccag catcagcggc gtagaactgc
     4981 gttccgccgt aacgggcgtg ggtttcgttt tggaagacga cggcgcgttc cactataccg
     5041 acacgtcggg ctcaaccatg ttctccatct gctcgctcaa caacgagccg tttaccaacg
     5101 cccttttgga caaccagtcc tacaaaggct tcagtatgct gctcgacatc ccgcactctc
     5161 cggcaggcga aaaaaccttc gacgatttgt ttatggattt ggcggtacgc ctgtccggcc
     5221 agttgaacct gaatctggtc aacgacaaaa tggaagaagt ttcgacccaa tggctcaaag
     5281 acgtgcgcac ttatgtattg gcgcgtcagt ccgagatgct caaagtcggt atcgaaccgg
     5341 gcggcaaaac cgcattgcgc ctgttctcct aaaatcgaat gcaatcaaag cggatatgtc
     5401 gttacggcat atccgctttt tttcgggtaa aattcagctg ttttcaacct atcatttttc
     5461 agacgacctt tgcccgtttc atcccgtatc agacaaaggt cgtctgaaaa ccttctctca
     5521 tgaatccaaa ctcaaaacat aacactaact ttaccaatct tcttaaaccg tctgatagtg
     5581 atataaaaca atttgccgca caacacatcc gccacctcac cgacctgctc aaccgctatg
     5641 cctacgaata ctacaccctc gacgcgccca gcgtacccga tgccgaatac gacaaattat
     5701 tccgtgaact cgaagcgttg gaattaaacc atcccgagct caaactgccc gacagcccga
     5761 cccagcgcgt cggcggcgag cctctggcgg gatttgccga agtacgccac gaagtgccga
     5821 tgctgtcgct gaccaacgcc ttttccccgc aagatgaaaa cggcgtgttc gaccatgcag
     5881 aaatgtacgc tttcgaccaa cgcgtgcgcg acggcttgga cggcggcaat cccgaatacg
     5941 ttatcgaacc caaattcgac ggcctcgcca tcagcctgct ctaccgcgac ggcgtattgg
     6001 tgcaggcggc aacgcgcggc gacggtacga cgggcgaaga cgttacccaa aacgtcaaaa
     6061 ccgtcgccaa catccccttg cggctgcacg gcgaaaatac gcccgaactc atcgaagtac
     6121 gcggcgaagt gctgatgcaa aaagccgatt ttgccaccct caataaaaga caagccgaaa
     6181 acgggcaaaa accctttgcc aacccacgca acgccgccgc cggcagcctg cgccaactcg
     6241 attcacgcat cactgcgcaa cgcaaattgc actttttccc ctactccgtc gcccgtcagc
     6301 aaggcggttt cgtcgcggaa gagcacatcc aagaactcgc ctatttccaa gaactcggct
     6361 tcagcctgcc taacggcaat ttcggctgtt tcaaaaatat cgacgaagta ttggcgtttt
     6421 acgaacaaat gcagcaaaaa cgccctgagc taccctatga aatcgacggc atggtcgtca
     6481 aagtcaacag cttggcgcaa cagcacgaac tcggcttcat ctcccgtgcg ccgcgttggg
     6541 cggttgccca caaattccct gccgaagaag ccctgaccat tgtcgaagcg attgacgtgc
     6601 aaatcggcag gacgggcgcg gtaacgccgg ttgcccgatt acaacccgta ttcgtcggtg
     6661 gcgtaactgt taccaacgcc accctgcaca atcaggacga agtatcgcgc aaagacgttc
     6721 gcgtcggcga taccgtcgtc gtacgccgtg ccggagacgt gattcccgaa gttgtgcgcg
     6781 tgatttttga acgccgcccg atgcgggaaa ccgccgttgc cgtttcagac ggcatcgggc
     6841 atcggcagga cgatttgttt gccgaaacac cgtccgctaa tcaaacccaa tccgttccgc
     6901 tccacaagcc ctaccgcctg ccgacccatt gccccatctg ccgcagcgaa atcgaacgcg
     6961 aagaaggcga agccgtcgcc cgatgcagcg gcggtatgct ttgtcaggca caacgcgcgc
     7021 aaggcttaat ccacttcgcc tcgcgcaaag cgatggacat cgacggcttg ggtgaaaaac
     7081 aaatcgaaca gcttgtcgcc caagacctcg tccgccactt cgccgacctc taccgcctcg
     7141 atattccgac cctgcaaaaa atgaaggaag cggcggacaa aaccgttgcc gaatcggatc
     7201 aaatgccgtc tgaaggcagt tcggtcggcg cgtcgggcaa acataaaaaa caaccgacca
     7261 agtgggcgga aaacatcctc gcaggcatag aatccggcaa aacgcccgaa ctcgcccgct
     7321 tcctgttcgc gctcggcatc cgccacgtcg gcgaacgcac cgccaaaacg ctggcacagg
     7381 cattcggcac attggaacgc gtccgccgcg cccccgaacc cgtcctcgcc tgcctgcccg
     7441 acatcggtac ggtagtggcg cgttccattg cccacttctt cgcccaagcc gaacagcagg
     7501 cgatgataga cgagctgctc gccgcaggcg ttgccccgca agcccaagcc gtcagcctgc
     7561 ctgccgcaca atatgccgaa ccgcaacgct ggattgcccg cctgcccggt ttcaaaatta
     7621 gcgaaaacaa agcccaagcc ttatgggaac tcgccggcaa aagtatagag ggtctgcaaa
     7681 ccgacaaatc cctccccgcc gactggcaag catggcgcag cgaaccacaa aacgccgccc
     7741 tactcgaaaa cctgaaaacc ttcttcgcgc aaatgccgtc tgaagacgag gcggcgcaag
     7801 gttcagacgg catcaataaa gccgtagcgg gcaaaacctt cgtgttaact ggcacactgc
     7861 ccaccctcaa acgcgaccaa gcccaatccc taatcgaagc cgcaggcggc aaagtttccg
     7921 gcagcgtgtc caaaaaaacc gattacgtcg tcgccggaga agccgcgggc agcaagctgg
     7981 aaaaagccaa tgccttgggc gtttccgtcc tcagcgaagc cgaactgctt accctgctcg
     8041 gctgaaccca tgccgtctga accgctttca gacggcattg cccacttttc cgaaccacac
     8101 catgcacacc atttcctttc caaaccgcac ccgactcacc gccctgccgc ccctgtcgct
     8161 ttacatccac atcccgtggt gcatcaaaaa atgcccgtat tgcgacttca attcccacag
     8221 cctgaaaaac ggattgcccg aagccgccta tatcgacgcg ctgctgaccg atttgcagct
     8281 tgaattgccc aatatttggg gcaggccggt ggaaacgatc tttttcggcg gcggtacgcc
     8341 cagcctgttt caggcggaat cgattgaccg tttgttaagc ggcgtgcgtt cgctgttgcg
     8401 cttgcagccg gaagcggaaa ttaccttgga agccaatccg gggacatttg aaatcgagaa
     8461 gtttcaagga tttaaagacg cgggtattac gcggctttct atcggcgtac agagtttcaa
     8521 cgacgatatg ctctcaaggc tggggcgcgt ccacaacggt aaggaagcct taaccgctat
     8581 cgataccgcc ttgaaattat ttgataaagt caatatcgac ttgatgtatg ccctgccgaa
     8641 ccaaaccgtt cagacggcat tggacgacgt acaaaccgct atcgctacgg gcgcgaccca
     8701 catcagcgcg tatcatctga cgatggaacc gaacacgccg ttcggtcata cgccgccgaa
     8761 aggtttgccg caagacgaag ccgccctcga catcgaagac gcggtacacg gcgcattgga
     8821 aaacgccggc tttatccatt acgaaacatc ggcttttgcg aaacccgcca tgcggtgccg
     8881 ccacaatttg aactgctggc agttcggcga ttatttgggc ataggcgcgg gcgcgcacgg
     8941 caaaatttcc taccccgacc gcatcgagcg caccgtccgc cgccgccatc ccaacgacta
     9001 cctcgccctg atgcaaaacc ggccgggtga agccgtcgaa cgcaaaaccg tcgccgccga
     9061 agatttgccg ttcgagttta tgatgaacgc cctgcgcctg accgacggcg tacccgccgc
     9121 gatgttgcag gagcgcacgg gcgtaccgag tgccaaaatc atggcgcaaa tcgaaacggc
     9181 aaggcaaaaa ggtttattgg aaaccaaccc cgccgtattc cgcccgaccg aacgtggacg
     9241 attgttttta aacgatttat tgcagtgttt tttataagcg caagcaaata caaggatgcc
     9301 gaaacggcaa attccaacaa cggacattct caaaaatgac gcgcggcaac ggcggcggcg
     9361 acaaggacgg aaccggcagg cggcggatac cggcggtttt cggtacgggg gcgtgccgaa
     9421 gatgcggggc ttatccttgc cgccgtggaa aaagccggcg gcagccgcct cccccgccgg
     9481 gcgtttcaga cggcatcgcc acgcacaaaa aaccgtatgc ctgcacgcgc gcaaattccg
     9541 tcatccacgc cgtccctcct tttcccaaac cgtttgcaag ttccgcccat ccgctgcgtg
     9601 atgccgctcc gtttaaaggc agggcacgga acaatgcccg gtatgccgtc ccgcatcttg
     9661 gggcattgaa aatattacga tgcaaaaaga aaatttcagt ataatacggc aggattcttt
     9721 aacggattct taaccatttt tctccctgac cataaaggaa ccaagatatg aaaaaagcac
     9781 ttgccgcact gattgccctc gccctcccgg ccgccgcact ggcggaaggc gcatccggct
     9841 tttacgtcca agccgatgcc gcacacgcca aagcctcaag ctctttaggt tctgccaaag
     9901 gcttcagccc gcgcatctcc gcaggctacc gcatcaacga cctccgcttc gccgtcgatt
     9961 acacgcgcta caaaaactat aaacaagtcc catccaccga tttcaaactt tacagcatcg
    10021 gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt caaaccgtat ctcggcgcgc
    10081 gcttgagcct caaccgcgcc tccgccaact tgggcggcag cgacagcttc agccaaatct
    10141 ccaccggtct cggcgtattg gcgggcgtaa gctatgccgt tactcccaat gtcgatttgg
    10201 atgccggcta ccgctacaac tacatcggca aagtcaacaa cgtcaaaaac gtccgtaccg
    10261 gcgaactgtc cgccggcgta cgcgtcaaat tctgatatac gcgttattcc gcaaaccgcc
    10321 gagcctttcg gcggttttgt tttccgccgc cgcaactaca caagccggcg gttttgtacg
    10381 ataatcccga atgctgcggc ttctgccgcc ctattttttg aggaatccga aatgtccaaa
    10441 accatcatcc acaccgacaa agcccccgcc gccatcggcg cgtacagcca agccgtgcgc
    10501 gcgggcgaca ccgtttacat gagcggtcag attccccttg atcccgccac gatgaccgtc
    10561 gtcggcaacg gcgatttccg cgccgaagcc cgccgggtct ttcaaaacct gcaagccgtc
    10621 gccgaagcgg cagacggctc gctggacgac atcgtcaaac tcaacgccta cctgaccgac
    10681 ttgggcaact tcgccgtctt caacgaagtg atggcggaat ttatcgccga gccgtttccc
    10741 gcccgcgccg ccgtcggcgt tgcctcgctg cccaaaggcg tgcaggtcga agccgaagcc
    10801 gtcctcgttt tgaacgcata attccacgtt cggactgcat gatatttcaa aaccatgccg
    10861 tccgaaccgc aacactttcc cacttcacaa catctgcaat ggcacattac gcaatcggcg
    10921 acatacaagg ctgtttcgac gaactgaccg cgctgctcgg caaaatcggt ttcaaccacg
    10981 gcacggacac cctttggctg acgggcgaca tcgtcaaccg cggctcgaaa tccctcgaaa
    11041 cgctgcaatt ctgcatccgg cacgaaaaca gcgtgcaaat cgtcctcggc aaccacgacc
    11101 tgcacctgct cgccgtcggc tgcggcgaag gcgcgcccaa acgcagcgac acaatcgaac
    11161 ccatactcaa acaccccgac ggaaaaaaaa tgctcgactg gctgcgcgcg caaccgctgt
    11221 tgatacgcga gggcagccgc gtgatggtac acgccggcat cctgccgcaa tggcgcatcg
    11281 ccaaagccga atcgctcgct agagaagccg aagccgaact gcgcggcaaa aaatacgtca
    11341 aattcttctc caaaatgtac ggcaacaaac cggccgcgtg ggacgaaggt ttggaaggct
    11401 atgcccgcct gcgcttcatc gtcaacgcct tcacgcggat gcgcgccctg acctttaaaa
    11461 acgaactgga tttcgactac aaatccacag tgaaaaaaat gccgccttac ctgcgcccgt
    11521 ggttcaaagc ccccgaccgg caaaacctcg accgcaccat catcttcgga cactggtcct
    11581 cgctgggcta cacgaatgcc gacaacgtca tctcgctgga caccggcgcg ctgtggggcg
    11641 gacagctgac cgccgtcaac ctcgaaacgg aagaaattac ccaagtccaa gccgccggcg
    11701 gcatagactg gaaaagcttc gcaaaataac ggaccgcccc actatgcaca aaatcctgcc
    11761 catcgcccac gtcctctccc gactgggtat gctgttttcc tttatcctgc tgatacccgc
    11821 cgccctctcc tacgcctttt cggacggcgc gtacaccgcc ttcgccacca ccgcgaccgt
    11881 taccctttcc ggctcgtgca tcgtccggct cgccaccctc cggttcaggc gcgaactgcg
    11941 cccgcgcgac ggctttaccc tcgtcctgat gttgtggctg gcgtttgccg ctatggctgc
    12001 gatgccgatg tacctgtatt tcccgaatat gggctttacc gacgcatttt ttgaatcgat
    12061 gtcgggactg accaccaccg gcgcgaccgt catcccccac gtcgacgggc tcgccccctc
    12121 cgtcaacttt tggcggcata tgctcaactg gctgggcggg atgggcatca tcgtccttgc
    12181 cgtcgccatc ctgcctatgc tcggcgtagg cggcacgcag ttattcaaag ccgaaatccc
    12241 cggcattgac aaagaaagca aaatgtcgcc gcgcatttcc caagtggcga aaaaactttg
    12301 gttcggctac accctgatca ccatcctcgc ggcagcctgc ctgcattttg ccgggatggg
    12361 ctggttcgat gccgtctgtc acgcgatggc gaccctctcg ctgggcgggt tttccaccca
    12421 cgatgccagc atagcttatt acaactcccc cctcatcgaa gcggtcatca tcgttttcac
    12481 cattgtcggc ggcatcaatt ttgccaacca tttcgccgcc ctcaacagcc gcagcctcaa
    12541 aacctattgg aaagacgaag aatgccggac gatgctgctg ctgctctccg gcagcatcct
    12601 tgccgccgcc ctgtacctgt ggcacaccgg ctattacgcc ggttttaccg aatccctgcg
    12661 ctacaccgcc ttcaacttcg tctccatcgg actggcaaac gggctggcca acaccgactt
    12721 cgcacaatgg ccgctcctga tttccctgtg gatgtttttc ctcgccaaca tcctcgccaa
    12781 ctccggctcg accggcggcg gcatcaaaac catacgcgcc ctcgtcctgt tcaaattcag
    12841 cctgcgcgaa atgatggtgc tgctgcaccc caaagccgtc cgcaccgtca aaatcagcgg
    12901 caaggccata cccgaccgcc tcgcgctgac cgttatgtcc ttcatcttca tctactttat
    12961 gatcgtcgtc ctcttcagct tcctgctgat ggcgagcggt atggaattta ccaccgcctt
    13021 caccgccgtc atcgcctgca tcaccaacgc cggccccggc ctgggcgaag tcgggcccgc
    13081 cggcaattac gccggtttgg acgtgatgca gaaatggatc tgcgttaccg ccatgctctt
    13141 gggcaggctg gaaatcttca ccgtcttcat cctcttcacc cccgcctact ggaagaaata
    13201 aaaaatgccg tctgaaaact tttcagacgg catattgtct tttaaaaaag cggaagctct
    13261 acaaaaggaa agaaatgctg aaacctctga taaatttcag gatatttctc taaggctttt
    13321 aatgcttttt cttttgaaat aggcggatca tagacatcta tcccccttaa gaaggcaatg
    13381 cccgttaatg catttttttg atcatttgaa atgtaaccat aattaacgac tccaattaca
    13441 tcataaattt cccctatact cttatcaaaa tcaatcaaat caaaaatata caatggaatt
    13501 gtatcaatag gagtatctct gacaactata tccaaccata atttaaattc agaaagatta
    13561 atagcatcat agaacataca gactattgca aatcctaaat cttcacaatt ttctttacta
    13621 attttccaca taattattta cctttatcca ttgcttccca accgatacat ccggtatttt
    13681 agcttctcct tttccgtcaa acactccttt ggttacctgc tttgtgtcaa aaataccgtc
    13741 tgaaagccga atatcgtttc agacggtatt ttgattgttg ggtttttaat cagagctttg
    13801 tttgataatt tgttattttg aataaaatac aggttctgtc gagctgataa cttctattac
    13861 agaatcgcta aaaaagaaaa tataagcctt ataatatttc ttttcaaaaa aatcaaaact
    13921 ttcttctttc aaccagtcca tataactatc atctattatc tcgaaaaaat aatttatagc
    13981 aggatttcta tcccaatcat ttttttcttt attttcaaat aaatattctg aaaattttac
    14041 tttataagat tcatccgtta tttgaaaatg aattatattg tcaataaata tttcaatatt
    14101 taattcataa tttcggtcgg atgccaaaat gttcacatct tgttcggtaa cacaaatttt
    14161 gtccacataa aatctgtgat tattgttaga aaattgtaat ggtttaatct tcacaatcgt
    14221 tttccatatc taatttttgt tttcttattg ccctgccgga taaagcaggt ctgcctccgt
    14281 actgtctatc ctcacattta ctttcctgcc atcggctttt atctccccgg ttttaccgtt
    14341 tttgccaact ttgcggcttt ggcggcggca atgttgaagg cggcttcgac ggtttcggcg
    14401 gcattggggt tgacccgtac tcatccgcaa acggcaacac gacggacggt gtttgctttt
    14461 cagccgtctg ccgttttgac caaggtgctg ccgtggaagg agcaggtgta ggatttttga
    14521 agaacttagt tcccaatatc ctgtttcaaa taaggggaag gtcgcctgaa aattaaacac
    14581 ggtttcagac ggcattttga ttgccggggt ttgctatttt ttgttgtaat aatcaaatcg
    14641 cacgttgact atgtctttct cggtaaaaat ataacggagc attgttgtga atctttcata
    14701 acgttcatga attcccacgc tatcaggtag ccaaggggaa gctttaattt cgaaaagctc
    14761 ccaatttgga accattaaaa aatcaataat ggtactgatt ccaatcacaa tatcccttgg
    14821 tatatccatc ggataaggat atttttttct aacctcgatt aaatcattct ccaatttcca
    14881 atattcttca tcatcccaca ccccgtcatc ataccatttg ccaataaatg aattttcgtc
    14941 atacccctca aaataaggaa cgtttcttat aatatccttg aactcacaca taataatgta
    15001 tctccaatat aattaaactt ttcatctcaa tctaccttta ctatgttgta ttggaaagta
    15061 aaaaaatttc cagtcctcta catctagatc agtaaaaata taacggagca ttactctgaa
    15121 cctttcataa cgctcattaa ttttgacact tttaggcaac caaggagaag ctttaatttc
    15181 aaaaagtttc caattttgaa ccattaaaaa atcaataatg gtaccgattc caatcacgat
    15241 gtcccttggt atatccatcg gataaggata tttttttcta acctcgatta aagcattctc
    15301 caatttccaa tattcttcat catcccacac cccgtcatca taccatttgc caataaatga
    15361 attttcgtca tactcttcaa aacaagggat gtttcttcta aaatccttga actcgcacat
    15421 aataattaat ctccaatacg atttaggttt ttatcaaatg taccgtttct tgtttctttt
    15481 ctgttcagat ttttgggtga agatgcttct ttccaagccc ctccattatg tgaatctaca
    15541 tcgcgtgata tataactctt tcctttttta aaaatagcag catcatttct cgttctttct
    15601 tttatttttc tatatcccaa tttctttgct gctgcatatg cttctgaatc attcccatat
    15661 acgggggtag atggtgtttt tcttggcgga cacgcattat gaacccaaac cccttccgtt
    15721 tccgcctgac tgcccttgac gaagtaagta tgccagtcgg caacggtcag attgtaggct
    15781 ttgagcggtt ttggtttgac aacgatgttg cggacggttt gggttttgcc gctttcggat
    15841 aacagcctgc ttcccgcctt taagtcttcc gccttaatcc atttgccgcc cgaataaaac
    15901 ggatggatgc ggttggaaat cagggtttgg ttgttgccga tgccgtctga aactttaatg
    15961 taaacggttt cttgatacgg attgccgtat cgggcggtaa cgggtttgta tcccgttttt
    16021 ccgcttgcct cgtccttgga gaagacgcgg tcgccggttc ggatacgggc aatggctttg
    16081 tagccgtctg ccgttttgac caaggtgctg ccgtggaagg agcaggtgta ggatttttga
    16141 agaactcagt tctcaatatc ctatttcaaa tgaaggaaat gccgtctgaa aattaaacac
    16201 ggtttcagaa gacattgtga ttgttggttt tttaacctac gcttgcgaaa ttaattcctt
    16261 tctcctgaat actgcaccat ttatttggtt taactccata gaagaatgat tagaataaat
    16321 acattcaaag tcagcttcat caaaatattc tccacacata gaccataact ctgtaatcaa
    16381 atattccata tcttcatggt cttctgtgat ggcttctctt gataaataat ttttgatttt
    16441 taaaaacctt cttccttcct cgtaatctac ggtaattaac ctgatatctg agtgtatcat
    16501 gcccagcatt gctatattca taccgactcg cagccagctt ggtaatatat tcataactat
    16561 tgcacctttt taatggtttt agggttagcc ggaacaatgt gaactccatt tttacctgaa
    16621 tgaatcgtac caaaattggt acttaatcct gatttcccat cgctgcctat aggataccca
    16681 aaatcaacaa caggatttcc tcttgctcct ttagaaataa ctggataagc acctgaatgt
    16741 attccgttca acaattcttg aggattaatg ttttgattta aagtactctt accttcaatg
    16801 tagtttctat gtcctgaaat atgtttccct tgagctccat catgaatttt agtcccaata
    16861 gattttgctt taaaatcaac attgcgggtt tgaaacattt ctccatctac agactgagat
    16921 aatcttgaag cagtgttatg cttgtaggag tctgagaaat ccccactaac cgcagccttc
    16981 cccggttttg ccgcctttgt caggtttttg actttggcaa acggcaggac gttgaccagg
    17041 gtttcgacgg tttcggcggc attggggttt tcctgcatcc actggtcaac ggcttcgccc
    17101 gcgctgataa aggggttaag cgcgccggcg gcgacgccgt tgataaactc catgctgttg
    17161 ccccagcggt cgagcttggc attgtgctcg aacatttttc tgttggcttc atcggcgcgg
    17221 tcggagaaat tgctgccgag gttgttgtag ttgtcggata tgcgttgccg gatgctgcgg
    17281 gtgtcggtcg gattgagttt gatgctgcgt gctgtgccgt tgacgtgata ggtgtattcg
    17341 tcgcgtgcgc ccgtaggttt ggggtaattg ccgcccttcg ggccgtcgta ggcatcggcg
    17401 ggatggtgct cgtgtccttc ccagttgagc cggtacacgg taaagccttc gtcaacgttg
    17461 ccttgttctt tgcttgcgct gtcggcggcg tggttgtcga agggggcgtg ttcttcgtgt
    17521 ccgtgtccgg aaaagcgggt gtggtagccg attgtgccgt tgatgtttgc ctgttggatg
    17581 agcaggttgc ccatctggtg ggtatagtct tggatgacgt tgattttgcc ggtgcggtcg
    17641 gaaacgctgc cgcgcgggtc gccgaagagg tggtatttgc cgccgggttc gtagtgctgc
    17701 cgttgggcgt tatcggtaat gaacgggtct tgcgccaagt ccgccgcgag ggcgggctgt
    17761 ccgaaggcgg cg
//
LOCUS       DAXHRI010000028        17102 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXHRI010000028 DAXHRI010000000
VERSION     DAXHRI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.028"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(123..1292)
                     /gene="metK"
                     /locus_tag="ACFM75_000710"
     CDS             complement(123..1292)
                     /gene="metK"
                     /locus_tag="ACFM75_000710"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC6255293.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1475..1585)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1638..2534
                     /locus_tag="ACFM75_000711"
     CDS             1638..2534
                     /locus_tag="ACFM75_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6255294.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELALAFFKKPEDIETMF
                     KSVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2578..3642)
                     /gene="tsaD"
                     /locus_tag="ACFM75_000712"
     CDS             complement(2578..3642)
                     /gene="tsaD"
                     /locus_tag="ACFM75_000712"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC6255295.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            3649..3774
                     /locus_tag="ACFM75_000713"
     CDS             3649..3774
                     /locus_tag="ACFM75_000713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255296.1"
                     /translation="MPLKTEKRILADFGTTAVSQKRNMPSEDRSDGIVAPYQSVP"
     gene            complement(3759..4946)
                     /gene="ccsB"
                     /locus_tag="ACFM75_000714"
     CDS             complement(3759..4946)
                     /gene="ccsB"
                     /locus_tag="ACFM75_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC6255297.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGDIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4939..6891)
                     /locus_tag="ACFM75_000715"
     CDS             complement(4939..6891)
                     /locus_tag="ACFM75_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC6255298.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7168..7791)
                     /locus_tag="ACFM75_000716"
     CDS             complement(7168..7791)
                     /locus_tag="ACFM75_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6255299.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7997..8662
                     /gene="yihA"
                     /locus_tag="ACFM75_000717"
     CDS             7997..8662
                     /gene="yihA"
                     /locus_tag="ACFM75_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC6255300.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKENLPQLYGNRI
                     SPT"
     gene            complement(8747..11143)
                     /locus_tag="ACFM75_000718"
     CDS             complement(8747..11143)
                     /locus_tag="ACFM75_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC6255301.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11295..12410
                     /gene="pilM"
                     /locus_tag="ACFM75_000719"
     CDS             11295..12410
                     /gene="pilM"
                     /locus_tag="ACFM75_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC6255302.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12413..13012
                     /locus_tag="ACFM75_000720"
     CDS             12413..13012
                     /locus_tag="ACFM75_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC6255303.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13013..13660
                     /locus_tag="ACFM75_000721"
     CDS             13013..13660
                     /locus_tag="ACFM75_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC6255304.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13678..14223
                     /locus_tag="ACFM75_000722"
     CDS             13678..14223
                     /locus_tag="ACFM75_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC6255305.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14242..16503
                     /gene="pilQ"
                     /locus_tag="ACFM75_000723"
     CDS             14242..16503
                     /gene="pilQ"
                     /locus_tag="ACFM75_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC6255306.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQTNIDFRKDG
                     KNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLK
                     RLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISL
                     DFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQ
                     QGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNAD
                     TTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVE
                     AADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINLPITAAANS
                     ISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIAN
                     GGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLN
                     TQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFIT
                     PRIMGTAGNSLRY"
BASE COUNT         4606 a         4848 c         3888 g         3760 t
ORIGIN      
        1 tttgcaaagg tctctatctg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
       61 atccactata taaaaatgcc gtctgaactg ttcagacggc atttttgatt ttcaaaccgg
      121 aattacagcc ccgctgccgc cctcaatgca gcagctttgt cggtgcgctc ccaagtgaac
      181 tcaggttctt cgcggccgaa atgtccgtaa gcggcggatt tactgtaaat cgggcgcaag
      241 agatcgagca tttggacgat gcctttgggg cgcaggtcga aatgttcgcg aactaaggta
      301 atcagttttt cctcgctgat tttgcctgtg ccgaaagtgt cgatggaaat cgaagtcggt
      361 tcggcaacgc cgatggcgta ggaaacttgg atttggcatt gggttgccaa accggcggcg
      421 acgatgtttt tcgcaacgta gcggcaggcg taagcggcgg aacgatccac tttggacggg
      481 tctttgccgg agaacgcgcc gccgccgtgc ggagccgcgc cgccgtaggt atcaacgatg
      541 attttacggc cggtcaaacc gcagtcgcct tgcgggccgc cgatgacgaa gcggccggtc
      601 gggttgatca ggtatttggt ttcgtcggtc agcagttcag acggcaggac gggcttgata
      661 atctgctcaa tgacggcgtt tttcaactct tcataaccga tttccgggtc gtgttgcgta
      721 gagaggacga cggtatcaat gcgttttact ttgccggatt cgctatcgta aaccacggtc
      781 agttgggctt ttgcgtcggg acgcagccaa ggcaggaggc cgtctttgcg taattcgctt
      841 tgacgctgca tcaggcggtg gctgtaatag atggcaaacg gcatcagggt cggggtttcg
      901 tcgcaggcat agccgaacat caaaccttgg tcgccagcgc cttggttcaa gtcaatgcct
      961 tcgccttcgt tcacgccttg ggcgatgtcc ggagattgct ggtcgtagta cacgccgacc
     1021 gcgcagccgt tggcatcaaa gcccagctcg gaggagttgt agccgatgcg tttgatggtt
     1081 tcgcgtgcga ctttgatgta gtccacttgg gcggtggtgg tgatttcgcc tgccaatacg
     1141 cacaagcctg tgttgaccaa agtttctgcg gcgacacgcg ctttggggtc ttgcgccaag
     1201 atggcatcca aaatcgcgtc ggatacttgg tcggcaactt tgtccggatg gccttcggat
     1261 accgattcgg aagtaaacag atattcgctc attgagattt tccttgaaaa acaaaccatc
     1321 ttcttcagac ggcatgttgt atgaacataa tgtcgacagc gggaaatata gcaaaatttc
     1381 cctattcata ccattcagtt gagaaatatt cccatttgaa tggcactttg gaatctctgc
     1441 ccgtacgttt cttacaggca aaaaaattcc cgcatcaagc gggtttggat tgctctggtg
     1501 agccacatcg gcttttcaac cgtccacctt acttttcctt ttgaaaagca ggttggcatg
     1561 gaattcccaa ctcttaatgc agcgcggatg gtagcagaaa agcctttccg ccgcaatact
     1621 tccctttttc agacggcatg tttcgtttac aattcgggct gtttccccct ttgcgaaccg
     1681 ccatgcacat cctgttgacc gccctgctca aatgcctctc cctgctgccg ctttcctgtc
     1741 tgcacacgct gggaaaccgg ctcggacatc tggcgtttta ccttttaaag gaagaccgcg
     1801 cgcgcatcgt cgccaatatg cgtcaggcag gcatgaatcc cgaccccaaa acggtcaaag
     1861 ccgtttttgc ggaaacggca aaaggcggtt tggaacttgc cctcgcattt ttcaaaaaac
     1921 cggaagacat agaaacaatg ttcaagtcgg tacacggctg ggaacacgtg cagcaggctt
     1981 tggacaaggg cgaagggctg ctgtttatca cgccgcacat cggcagctac gacttagggg
     2041 ggcgatatat cagccaacgg ctgccattcc ccttaaccgc catgtataaa ccgccgaaaa
     2101 tcaaagcgat agacaaaatc atgcaggcag gcagggttcg cggcaaagga aaaaccgcgc
     2161 ctaccagcat acaaggggtc aaacaaatca tcaaagccct gcgttcgggc gaagcaacca
     2221 tcgtcctgcc cgaccacgtc ccctcccctc aagaaggcgg ggaaggcgta tgggtggatt
     2281 tcttcggcaa acctgcctat accatgacgc tggcggcaaa attggcacac gtcaaaggcg
     2341 tgaaaaccct gtttttctgc tgcgaacgcc tgcctggcgg gcagggtttc gatttgcaca
     2401 tccgccccgt ccaaggggaa ttgaacggcg acaaagccca tgatgccgcc gtgttcaacc
     2461 gcaatgccga atattggata cgccgttttc cgacgcagta tctgtttatg tacaaccgct
     2521 acaaaatgcc gtaacgaaaa taaaaatgcc gtctgaacaa tttcagacgg cattttgtca
     2581 tctgacgatt tccgacagcg gccagcgcgg acggacattg aacgcaccga cctccctgcc
     2641 cttgcccagg tgcatcgcac cggcaaaggc aatcatggca ccgttgtccg tgcagtatgc
     2701 catcggcggg aaaaacacgc tgactttttc ggacggatgt ttcggcttgc ctttgggggt
     2761 cgggatttgc accgtcatgt tgccgaaagt ttcacggagc ttgcggtttg caccgacccc
     2821 gccggcgacc actacggttc tgaaccctgt ctgcaacagg gcttttttca ctttcgccgc
     2881 caacacatcg actaccgcat cttgaaacgc acggcagatg tcgttgcgtg tctgctcagg
     2941 aatgtcatcc gccccgtttt ccgcgcgcac tttctcgacg gcggtcaata cggcggtttt
     3001 caaacctgaa aaactcatct gcaaatcgtc ggaatgaatc atcgggcgcg gaaaaacaaa
     3061 cgcttcgaac ctgcccgatt ccgcaagttc cgacagtttc gcaccgcccg gatacggcaa
     3121 gcccagcagt ttcgccgttt tgtcgaatgc ctcgcccgcc gcatcatcga cgctctcgcc
     3181 caaaagcgcg tagtcgccta tgcccctgac cgccataatc tgcgtatgcc cgcccgaaac
     3241 caacagcgcg acaaaaggaa agtcgggttt ttcctccgcc aacagcggcg acagcagatg
     3301 tccttccaaa tgatggacgg ggatgacggg cttgcccaat gccaaagcca gtgcattggc
     3361 gtagctcgaa cccgccagca gcgcaccgcc caaaccaggc ccctgcgtaa aggcaaccgc
     3421 gtcaatgtcg ccatacgatg cgcctgcctg cgccaaacag ccttcagtca acggaacaag
     3481 gcggcggata tggtcgcggc ttgccaattc cggcacaacc ccgccgtatt cggcgtgcat
     3541 tgccatttga gtgtgcaggc agtgcgcccg caatccacgt tccgtatcgt aaagcgcaac
     3601 acctgtttcg tcgcaagaag actcgattcc taataccaac atggtctgat gccgttaaaa
     3661 actgaaaaac gtattttagc ggatttcggc acgactgccg tatcccaaaa acggaacatg
     3721 ccgtctgaag accgttcaga cggcatcgtc gcaccgtatc aaagcgttcc gtaagaatgc
     3781 agcccgctca aaaacatatt cacgccgata aaggcgaatg cggttacgaa caaaccgata
     3841 atcgcccacc acgccagcac tttgccgcgc caaccggcaa ccagccgcaa gtgcagccaa
     3901 acggcgtaat tgagccagac gatgaacgcc cacgtctctt tcgggtccca actccaatag
     3961 cgtccccaag catccgccgc ccacagcgca cccaaaatgg tggcaatggt aaagaacaga
     4021 aagccgacgg caatcgcctt atacataact tcctcaatca acacggattg cggcagccac
     4081 gatttcccgc cttttccttc cgcacgcagg gaaaccagtt cggcaatacc gagcatcgcg
     4141 gaaatgcaaa acgcgccgta accgataaag tttgccggaa cgtggatttt catccaccaa
     4201 gattgcaatg ccggaatcag cggctggatg gtatgcgcct cgcgggacac gctgtaccac
     4261 aagacaaatc caaccacgac cgccataaag ccgaacacga agccgcccaa tttctgtatg
     4321 gcgaacttac cttcataata aagatacatc agcgcggtaa tcaccaaaaa caggatgaac
     4381 acttcataca ggttggaaac cggaatatgc cccgcatcgg gacggagcag atagctttcg
     4441 tgccaacgta ccagcagacc ggtaaagcct gctacggcag acacccatgc aaacacggtt
     4501 cccataccca acagcgtgtt ggtcggcaca ttttttacgc ttgccaaaac cgcgcccgaa
     4561 atataggcga acagggcgaa gaagacaaag gcgcactgcc acataatcgc cgactggctg
     4621 ctgaggaaat accgcaacag gaaaatctct gccgatttga tgtcgccgcc atacaaaccg
     4681 acggcggcat aggcaagcaa tacgcttaaa ggaacaaacc agcgcatcgg tttgaaaaac
     4741 caacccaaaa acacggcaat accggcactt gcccacaaca tgaccgtttc gtaaatgtcc
     4801 atatgcatac cggaacgggt ctgtacgaaa accgtagccg caaaaaccag cacggcaaat
     4861 acccaatccc aaagattcag attgctgatc aaagacttct gaatcagcag ctcgtgttcc
     4921 ggaagggttt tatagtgttc agtcatgatt caagtccttg ccgagccgtt gcagactctc
     4981 gacgtgtttt ggaaattcct tctgcaaatc ccgttcactg cgggccgaag acatggcaaa
     5041 acggattttg ccgtctgaaa acaataccca cgcccgtttt tcgcgcacat aaaacatcaa
     5101 taccgtaccc aataccaaca gcaccgagcc gagatagacc aaaagcgcac ccggggaacg
     5161 ggtcatctgc aaacccgacg aacgcacctc ggaaaacccg tcaagttgca gcagcatagg
     5221 cgcgggatat tcggtcaaac ccgtgtacgc atccatactg tgcagcagga aacggttccg
     5281 cgcttcatcc tgctgccatt cgggcaagcc gtaccggcgt atggtttcat ccaaagcagc
     5341 gttcatcacg ccgtaaagca tttcgtagaa ataaccctgc atcttatcct gctgctcttt
     5401 cgggatattg gacgtaataa attcgtccaa tcccaaatag cctttttgtg caaagatgtt
     5461 cagcgtgttt tccgcagcca gcatgaattg ttcgcggatt tcggcaggtg cgcctttggt
     5521 tgcgtcggca accagacgtt tgcgcccttc cccatctttc aaaaactcac gcaatgccat
     5581 aaaggtgtcc gctttcaact gcttgtccaa ggggatacgc agccagcggt attgctgctg
     5641 caagccgctg cgcgtgccgg taatccaaaa ataatcctgt tcctgcaaaa ccggcagcat
     5701 atagttttta tattcgaccg cctgccctgc cgcatcacgg atacggtaaa caatggaagg
     5761 gccgatattg gtgtattttt taccttcctg agtaacggcg cggacatcgt tcagcgtgga
     5821 tttcaggctt ttttcccgtt ccgcgccctc gctcatgtcc tccacattca tagaagtgaa
     5881 ctgatcgaac tcaagacgat atttgtgttt gccaatttcc aacggaaact ggtgtatgga
     5941 tgttgccttc aacacgacag gctcgcgcga agcatcaccc aaattccacg ccttgaatgt
     6001 caaatccgaa ccgccgtcgg caaaactcgc ctgataaatc gtgatgccgt gcaaggtcaa
     6061 aggatggttc acgcggatgg tgcgctcgag tttctcaccg gttgccttgt ccgtcacttc
     6121 aatatcgctg gcgaaatcac gcggcatacc cgtattgtaa aaatcgatat ggaatttttt
     6181 cagtttgact tcaaaaggca agtcctgaac caatatcccg ttgtcggcat tcaggaaaac
     6241 cacatccgca ctctgcccct cggaaatatt gacgttgccc ctaaatgaga gattggacgc
     6301 acccaaaata ctttcgggct tgaaatcctt ggcataaacc gcctgattgt ccggaacaat
     6361 ccgaccggtc agcataccca gtttcaacag caggttactg tctatcaacc cgcccaggca
     6421 aatgacaatc aaagcaacat gggcaaagat atagccccat ttgttcattg tgcctttttt
     6481 ggcggcaatc agaaccgacc cgtcttcacg gttaatggtt tttccctgaa aaccttgtac
     6541 ttccagataa cgtttggcaa cctcgggcgc aatttttaca tccaacagcg aagaatggcg
     6601 catcgccgcc agagattttt ctttaacctt ttcccgaaaa gacttcattt cgcgccagaa
     6661 cggcggcaca ttgcgaatca ggcacaaact ggtagaaacc accaaaaaca tcatgataac
     6721 gacaaaccat gccgaagcat agacgtcata cagtcccaga aaaccaaaaa tctgcgccca
     6781 aaaaggtccg aatttgacca aataatccgt ctgcggctgg ttttgctgca acaccgtacc
     6841 gataaccgat gcaataccca gcagactgag caaagcgacc gcaaagcgca tggagctgaa
     6901 aaaagcgaac cacggacggg aaagaagtgg gggagatcta cgggatttac tcattgtgtg
     6961 tttattccgc catcaggaat atgggaaagc agaattgggc aaacagaaaa caacgtcccg
     7021 attctactgt cttgatgctt tttatttcaa gacaatgaag acagcctgca tcgattccaa
     7081 cggttgcgat tgaaaaaact tatcgcagaa ttgcctgaag ccgtctgaaa acttttcaga
     7141 cggcctctaa aacagactat tgcggaatta acgcaaacct tggataaagt tggcgaccgc
     7201 tttcaaatct tcttcagaca tacggtttgc aatatcttcc atgatggtat ttttacgctg
     7261 accggacttg taggcattca tctgttcaac aatatatgcc tgatgctgac cgcccaaacg
     7321 cggataagcc tgaatttcgc ttccgcctcc cggcataccc gcaccgctcg gaccgtggca
     7381 ggacatacac gccggcactt ttttatcgct caaaccgccg cgatagattt tcgcacccaa
     7441 ttcgggattt tccttaggat tggcttcacc ggatttgggc tgctgtttgg catagaatgc
     7501 ggatacgttc aaaatatcct gatcgctcaa attcattacc accggtttca tcacagctgc
     7561 cgaaccgtgg gtgcgtttac cgtcgcggat gccgatagtt tgatgataga tgtaagcagt
     7621 atgctgtgcc gccaaacgcg gatacatcgc aatgccgctg ttaccgtctg ctgcatggca
     7681 agccgcacaa accgttgcgg caacctgttt gcctttttcc acgtctgctt tgggagatgc
     7741 ggaaaccgca ccggcagcca aaacaaaggc caataaagtc aatcgtttca tggagtgctc
     7801 ctgattacag cattggataa cgcaacaatg ctctttttat attcaaatac gggatttttg
     7861 acccgattaa aaccgatgat tctgcaaacg tgttattcta tactaaattt acattaaatt
     7921 accactgtgt ttcacataaa accaaccgca tatttttgct gtcggacaaa cggcggcgga
     7981 aaacaaggat atgcccatga acctttttca aaacgccaaa ttcttcacga cggtcaacca
     8041 ccttaaagac ctgccggaca caccgctcga aattgccttt gtcggcagga gcaatgccgg
     8101 aaaatccagt gccatcaata ccctgaccaa ccatgtccgt cttgcctacg tttcaaaaac
     8161 accgggacgg acgcagcata tcaacttttt cgagctgcag aacggcaatt ttatggtcga
     8221 tttgcccggc tacggttatg cccaagtccc cgaagcagta cgcgcacatt gggtcaatct
     8281 gctcggcgac tacctccgcc atcggaaaca gcttatcggg ctggttttga ttatggatgc
     8341 ccgccatcct ttaaaagaac tcgacatccg tatgctggat tttttccaca cgaccggcag
     8401 accggttcac atcctgctgt caaaagccga caaattatcc aaaaacgaac agataaaaac
     8461 cctgtcccaa gtcaaaaaac tgctcaaacc ttattccgac aggcaaaaca tcagcgtaca
     8521 gctgttttcc agcctgaaaa aacaaggtat tgacgaggcc aaccgaactg tcggaagctg
     8581 gtttgacgca gcagatgccg ccgcttcctc tccaaaggaa aacttacccc aattatacgg
     8641 aaaccgtatt tcccccactt gaccgaccgc aaacatttaa aaaattgcca ctgccaaatc
     8701 taaaatgccg tctgaaaagt ctttcagacg gcattttgcg gagtctttaa aacagagaat
     8761 ccaactgctg ctgtttggaa ccagtattac tcggaagcac cggcgtttcc tgcatatctt
     8821 ggcggacttc gtcatccgcc gcctgccgtc cgccttctgc cgcgcccccg tcatcttctt
     8881 ttgcccgtcg ggaaggttgc ggcgcaatac cgctgttgtc cagcgtcaag cccggatcgg
     8941 ttaccatacg ttccttcata tagtattcgc cattgctgct gaccacacct tcaggcattt
     9001 tcatcccctt gccctgcttt cctttcaacg caaaacgcat atagtccacc caaaccggca
     9061 ccgcaatcgt accgccgtag ccgacacgcc ccatactctt aggtttgtcg aagccgatat
     9121 atacggcagt aaccacatca gggttaaaac cgacaaacca cgcatcctta ttgtcattgg
     9181 tcgtacccgt tttaccggca atatccgttc ttcccaacgc agctgccccc cttgccgtac
     9241 cgacacggac cacatcctgc ataatcttat acataatata ggcattgcgc ggatcgattg
     9301 cctgaggcgc attttgcccg gccaccaaag gttgcatttg ggcgcgcaac ctgccgtctc
     9361 tgtcataaat cttatcgatt acgtgcgaag aaaccctata tccgccgttc gcaaatacgc
     9421 tatatgcctc cgccactttc aacggcgttg tctcgcccgt acctaaagcc atagacaggc
     9481 ttgccggcag ctcggacgac ctgaagccga aacgccggat atactgttgc gcgtaaccga
     9541 caccgataga catcaaaata cggatggaaa ccatattctt ggaagccgtc agagcctgtc
     9601 tcaaagtaat gtagccggaa tatctgccgt ctgaattttt aggtgtccaa accgaaccgt
     9661 tcggcccttt ccccggcagg gaaatcggcg catcgttaac cactgtggac gcggtcatcc
     9721 ccttagataa tgccgccgaa tagacaaacg gcttaaaggt cgaacccggc tgccgcattg
     9781 cctgaacggc acgattgaat gttttgctgt gaaaatcata accgccgacc agcgcgcgca
     9841 cagctccggt ttttgcatcc agcgaaacca aagccccctg cagcaacggc tcttgaacca
     9901 ccgcccaacg cccgccgttg tttttgacac ggatgaccgc gcccctgcgg atacggtcct
     9961 cccccatttt ttcattattg accgcgcggg ccgcaaagcc caaggcgcgc ctatcaagcg
    10021 taacccgcct gccgccgggc agctgtatga cgacattttt ctttttagtc acatccaaca
    10081 caacggcggg aaccatttta tcgacggtat agagtcccga cagatactgg ctgacagtct
    10141 cctcgacatc ttcactctta ctcaaatcga tatagttttc cgcaccgcgg tagctgctgc
    10201 cgcgatcgaa attccgtaga gccttgcgca atgcctcggt tgccaccttc tgatgatcgg
    10261 cgcggaccgt ggtataaacc ttaaaaccct gcgtataggc atcttcaccg tatttctcat
    10321 acagttcctg acgcaccatt tccgccacat ataaagcact ctgatcgatt ttccgaacaa
    10381 accgctcgta atgcagttcc tcattcaacg cctgatcgcg ctgttgcacg gtaatcatct
    10441 tctcctcgag catattgttc aaaatatact tctggcgcaa cttggcacgt tctggattaa
    10501 caatcggatt ataggcagac ggagccttgg gcagtcccgc aagcatggcg gcttccgcca
    10561 aagtcaaatc tcggacattc ttattgaaat agatttgcgc ggcagatgca aaaccatagg
    10621 cgcgctgacc gaggtaaatc tgattgaaat acaactcgag gattttgtct ttgcttaaag
    10681 actgctcgat tttataggca agcaacacct cattgaattt gcgtgtgaac gttttttcac
    10741 tgctcaaata aaaatttttc gccacctgct gcgtaatcgt actcgcaccc gactgcacgc
    10801 tgccggacac gacattgccg acggcagcgc gggcaacacc ccaaacatcc accccccaat
    10861 gccggtaaaa gcgtttgtcc tcggcggcga taaccgcatt ccgcaacacc tctgggaaat
    10921 cgccgatttt tgtaaattcg cgccgctgct ccccatacat accgatgact tccccatccg
    10981 ccgaataaat agtcaacggc attttaggct ggtaatgctg caaagaatcc aaagacggca
    11041 gtttcggata cgttaccaaa atagcaatgg caaccaaacc cactccaaat acacaaaacc
    11101 caaaaaccaa accaaaacaa gtcgttaaaa tctttttaat catagctgaa taataattta
    11161 ccattattgg tattaaataa agtaaaatag caaccgattt ctacaaagca cggtttcaat
    11221 gtgcaaagaa caaggaatcc attacggata ccgaaacggt tactcactat acaaataaag
    11281 caggaacttt catcatgcgc ttgtttaaaa gcttgaaaaa ccctaaaaaa acagatgcca
    11341 agctccctaa aaaatcttcg ggactcaata accgcgcggc aatcggcatc gatatcgacc
    11401 agcattccat caaaatggtc caattgtcag gacgtagttt aaaccaaatt caattggaaa
    11461 aatacgtcat tgccaaatta ccaaagaata tcattcaagg caataaagtc caaaattacg
    11521 atcaacttgt tacatatttg caacaagcct atgccaaact gggtacttcg tgcaaaaaca
    11581 tcatcgcgtc cgtcccgcaa aatctggcaa ccatcgaaca attgacctac acagacaaag
    11641 atgcagaatt agacctgcag gggttcgtgg agtcctccat ctccgaagtc agctcgatat
    11701 cgctcgaaga agccaattac gactatcagg tcttgtccca atcggccgcc ggcgaagctg
    11761 tgttggccgt cgcatcgaga aaggatgaaa tcgaacccct gattgacgca ttcaacgccg
    11821 ccggtatgaa attatccgcg cttgatgtgg acattttcgg acaatacaac gcctacgcgc
    11881 tatggataaa ccatttcgcc cccgagcttg cagccgaaaa agtcgccatt ttcggcgtat
    11941 atgccgcaca gacctacgcc ttggtcatcc aagacggaaa aatcctatac aaacaggaaa
    12001 cctccgtcag cgaagaacag ctcaaccaac tcatccagcg cacctatcag gtaacagaag
    12061 aaaaagcgga agaaatcatc aactccccgc aaaaaccttc cgattaccaa gaaagcgtgg
    12121 caaactattt caaccagcag attacccaag aaatacaaag ggtcttgcag ttttattaca
    12181 ccacgcagac cgcagacgat atgaccgaca tcaagcatat cctgctgacc ggggaagcgg
    12241 cgcgccagga aggcatcgcc caaaccgtcg cctcacaaac caatgcagat gtacaatgcg
    12301 tccatcctgc gcgttatttt gcggacaacc tcaaaacaga caaacaacaa ttcgaacttg
    12361 atgcgccgac actgaccagg gcgttcggtt tggcggtacg gggattataa ttatgaacaa
    12421 tttaatcaaa atcaacctcc tcccctacag ggaagagatg aacaagcgca aacagcagca
    12481 gtttaaaacg ctgatgtacg gtgccgtgct gacgggcgtt gccgccgttg cggcaaccta
    12541 cctgtttatc gacaatatga tcaataacca gtcggaaaga aacacgctgc tggaaacctc
    12601 catcgcacac ttggataccg agctgtcgga aatacaaaag ctcaaacagg aaaaagatgc
    12661 cttcctgatt aagaaaaaca aaatcgagga gctccagctc aaacgcctcc aagccgcaaa
    12721 aatcctcgac agcctgaatg aggccgtccc cggaagcacc tacctgacct cgctggatgc
    12781 cgttaccgcc gactcttatc ggctcagcgg caggacatcc agcgacaacc gcgttgccgc
    12841 catgatgagg gcgatgccca ataccggcat attcaagcaa cccgaattgt taagcatcaa
    12901 gaaaaacaat tcgcatcaag aatttaccct tcaggcaaca ttacaaccca tcgtaaaggc
    12961 ggccgaatcc aaagagaatc cggcttcggg aaacgcacag gaggcaaact gaatggcttc
    13021 taaatcatct aaaaccaact tggatctcaa caaccttcac ctgctcaacc ttcctgccag
    13081 gctttttatc gccctgctgg ccgttgccgc cgtgctgggg ctcggttatg ccggattgtt
    13141 caaaagccag atggaatccc ttgaggaata cgaagcaaaa gaaaccgaac tgaaaaacac
    13201 ctacaaacag aaaagtatcg acgcggccag cctgaacaac ctgagggacg aacttgcctc
    13261 aatccgctct gccttcgata tcatgttgaa acagctgccg acagatgcag aaattcccaa
    13321 cctggttcaa gagcttcatc aggcaggttc gagcaacggc ctgcgcttgg acagcgttat
    13381 gccccaacct cccgtagatg acggccccat caaaagatta ccctattcca tttccattac
    13441 cggaaattac gaacagatca gccaatttac ccgcgatgtc ggcagcctct cccgaatcat
    13501 tacccttgag tcgctgaaaa tcgcccaatc tccggaaaac ggcggcaatc ctgacggcaa
    13561 gagcagcatc ctgaacctca gtgccattgc caccacctac caagcaaaat ccgtagaaga
    13621 gcttgccgca gaagcggcac aaaatgccga gcaaaaataa cttacgttag ggaaaccatg
    13681 aaacactatg ccttactcat cagctttctg gctctctccg cgtgttccca aggttctgag
    13741 gacctaaacg aatggatggc acaaacgcga cgcgaagcca aagcagaaat catacctttc
    13801 caagcaccta ccctgccggt tgcgccggta tacagcccgc cacagcttac agggccgaac
    13861 gcattcgact tccgccgcat ggaaaccgac aaaaaagggg aaaatgcccc cgacaccaag
    13921 cgtattaaag aaacgctgga aaaattcagt ttggaaaata tgcgttatgt cggcattttg
    13981 aagtctggac agaaagtctc cggcttcatc gaggctgaag gttatgtcta cactgtcggt
    14041 gtcggcaact atttgggaca aaactacggt agaatcgaaa gcattaccga cgacagcatc
    14101 gtcctgaacg agctgataga agacagcacg ggcaactggg tttcccgtaa agcagaactg
    14161 ctgttgaatt cttccgacaa aaacaccgaa caagcggcag cacctgccgc agaacaaaat
    14221 taagaagagg attactccat tatgaatacc aaactgacaa aaatcatttc cggtctcttt
    14281 gtcgcaaccg ccgcctttca gacagcatcg gcaggaaaca ttacagacat caaagtttcc
    14341 tccctgccca acaaacagaa aatcgtcaaa gtcagctttg acaaagagat tgtcaacccg
    14401 accggcttcg taacctcctc accggcccgc atcgccttgg actttgaaca aaccggcatt
    14461 tccatggatc aacaggtact cgaatatgcc gatcccctgt tgagcaaaat cagtgccgca
    14521 caaaacagca gccgtgcgcg tctggttctg aatctgaaca aaccgggcca atacaatacc
    14581 gaagtacgcg ggaacaaagt ttggatattc attaacgaat cggacgatac cgtgtccgcc
    14641 cccgcacgcc ccgccgtaaa agccgcgcct gccgcaccgg caaaacaaca ggctgccgca
    14701 ccgtctacca agtccgcagt atccgtatcc aaacccttta ccccggcaaa acaacaggct
    14761 gccgcaccgt ttaccgagtc cgtagtatcc gtatccgcac cgttcagccc ggcaaaacaa
    14821 caggcggcgg catcagcaaa acaacagacg gcagcaccag caaaacaaac caatatcgat
    14881 ttccgcaaag acggcaaaaa tgccggcatt atcgaattgg ctgcattggg ctttgccggg
    14941 cagcccgaca tcagccaaca gcacgaccac atcatcgtta cgctgaaaaa ccataccctg
    15001 ccgaccacgc tccaacgcag tttggatgtg gcagacttta aaacaccggt tcaaaaggtt
    15061 acgctgaaac gcctcaataa cgacacccag ctgattatca caacagccgg caactgggaa
    15121 ctcgtcaaca aatccgccgc gcccggatac tttaccttcc aagtcctgcc gaaaaaacaa
    15181 aacctcgagt caggcggcgt gaacaatgcg cccaaaacct tcacaggccg gaaaatctcc
    15241 cttgacttcc aagatgtcga aatccgcacc atcctgcaga ttttggcaaa agaatccggg
    15301 atgaacattg ttgccagcga ctccgtcaac ggcaaaatga ccctctccct caaagacgta
    15361 ccttgggatc aggctttgga tttggttatg caggcacgca acctcgatat gcgccaacaa
    15421 gggaacatcg tcaacatcgc gccccgcgac gagctgcttg ccaaagacaa agccttctta
    15481 caggcggaaa aagacattgc cgatctgggc gcgctgtatt cacaaaactt ccaattgaaa
    15541 tacaaaaatg tggaagaatt ccgcagcatc ctgcgtttgg acaatgccga cacaaccgga
    15601 aaccgcaata cgcttgtcag cggcaggggc agcgtgctga tcgatcccgc caccaatacc
    15661 ctgattgtta ccgatacccg cagcgtcatc gaaaaattcc gcaaactgat tgacgaattg
    15721 gacgtacccg cgcaacaagt gatgattgag gcgcgtatcg tcgaagcggc agacggcttc
    15781 tcgcgcgatt tgggcgttaa attcggcgcg acaggcaaga aaaagctgaa aaatgataca
    15841 agcgcattcg gctggggggt aaactccggc ttcggcggcg acgataaatg gggggccgaa
    15901 accaaaatca acctgccgat taccgctgcc gcaaacagca tttcgctggt gcgcgcgatt
    15961 tcctccggtg ccttgaattt ggaattgtcc gcatccgaat cgctttcaaa aaccaaaacg
    16021 cttgccaatc cgcgcgtgct gacccaaaac cgcaaagagg ccaaaatcga atccggttac
    16081 gaaattcctt tcaccgtaac ctcaatcgcg aacggcggca gcagcacgaa cacggaactc
    16141 aaaaaagccg tcttggggct gaccgttacg ccgaacatca cgcccgacgg ccaaatcatt
    16201 atgaccgtca aaatcaacaa ggactcgcct gcgcaatgtg cctccggtaa tcagacgatc
    16261 ctgtgtattt cgaccaaaaa cctgaatacg caggctatgg ttgaaaacgg cggcacattg
    16321 attgtcggcg gtatttatga agaagacaac ggtaatacgc tgaccaaagt ccccctgttg
    16381 ggcgacatcc ccgttatcgg caacctcttt aaaacacgcg ggaaaaaaac cgaccgccgc
    16441 gaactgctga ttttcattac cccgaggatt atgggtacgg ccggcaacag cctgcgctat
    16501 tgatgcgtca aaataagggc atatgtttta cggcatatgc cctttcttta tgctttttgc
    16561 cgcgaccgaa atgccgtcat tcccgcgcag gcgggaatcc agtccgttca gtttcggtca
    16621 tttctgataa attcctgttg cttttcattt ctggattccc acttttgtgg gaatgacggc
    16681 ggaaggggta aatcctcgca atccaaagcc tcgtcatttc cacaaaaaac agcaacccgg
    16741 aacagcaact taaaaccccg tcattcccgc gcaggcggga atccagtccg ttcagtttcg
    16801 gtcatttccg ataaattcct gttgcttttc atttctagat tcccactttt gtgggaatga
    16861 cggcggaagg gataaatcct cgcaatccaa agcctcgtca tttccacaaa aaacagcaac
    16921 ccgaaacacc ccgtcattcc cgcgcaggcg ggaatctagg tctttaaact tcggtttttt
    16981 ccgataaatt cttgccgtat tgaaattcta gattcccgct ttctcgggaa tgacgggata
    17041 tgggtttccg tgcggacgtg ttcggatttc cgcctgcgcg ggaatgacgg cgacagatgc
    17101 cc
//
LOCUS       DAXHRI010000029        16494 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXHRI010000029 DAXHRI010000000
VERSION     DAXHRI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.029"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(27..1136)
                     /gene="mltB"
                     /locus_tag="ACFM75_000724"
     CDS             complement(27..1136)
                     /gene="mltB"
                     /locus_tag="ACFM75_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC6255307.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGSGL"
     gene            complement(1045..1347)
                     /locus_tag="ACFM75_000725"
     CDS             complement(1045..1347)
                     /locus_tag="ACFM75_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255308.1"
                     /translation="MRIAGKYGGRQCRLKGEVPLDGIFGGGRQISGGAKGNARHSAKP
                     PIAADTAFRAAPLRRFPTYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1338..2765)
                     /locus_tag="ACFM75_000726"
     CDS             complement(1338..2765)
                     /locus_tag="ACFM75_000726"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6255309.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLGRYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDAAACLLNDIRKDILDCRYCG"
     gene            2950..7062
                     /gene="mfd"
                     /locus_tag="ACFM75_000727"
     CDS             2950..7062
                     /gene="mfd"
                     /locus_tag="ACFM75_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC6255310.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHTLPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQTEFPASATNPLPSPLPQEREQSAAAVSDDLKAKSSLHLVASNLH
                     GQIRQQPTPSPVGEGWGEGKAVSAQTEFSAAAISPLPSPLPQEREQSAAAVSDDLKTK
                     SSLHPVASNLHGQIRQQPTPSTVGEGWGEGKAVAAQSAISVITESDLYQYVARSRVHN
                     RRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLVTIDLGVETNEMMLLEYAGE
                     AQLYVPVSQLHLISRYSGQAHENIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAR
                     RAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGF
                     GKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSK
                     ATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRAN
                     VDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLREL
                     KRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNV
                     LLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITK
                     DAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQA
                     VRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCETVQQIN
                     AIHEELVDRFGLPEQAVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPT
                     EIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            7211..7594
                     /gene="panD"
                     /locus_tag="ACFM75_000728"
     CDS             7211..7594
                     /gene="panD"
                     /locus_tag="ACFM75_000728"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC6255311.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7616..8458
                     /gene="kdsA"
                     /locus_tag="ACFM75_000729"
     CDS             7616..8458
                     /gene="kdsA"
                     /locus_tag="ACFM75_000729"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6255312.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8473..8913
                     /locus_tag="ACFM75_000730"
     CDS             8473..8913
                     /locus_tag="ACFM75_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC6255313.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     SVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWQLSGKEAV"
     gene            8960..10246
                     /gene="eno"
                     /locus_tag="ACFM75_000731"
     CDS             8960..10246
                     /gene="eno"
                     /locus_tag="ACFM75_000731"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC6255314.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            10261..10539
                     /gene="ftsB"
                     /locus_tag="ACFM75_000732"
     CDS             10261..10539
                     /gene="ftsB"
                     /locus_tag="ACFM75_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC6255315.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNNNLKNQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10580..10870)
                     /gene="yfaE"
                     /locus_tag="ACFM75_000733"
     CDS             complement(10580..10870)
                     /gene="yfaE"
                     /locus_tag="ACFM75_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC6255316.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(11068..12201)
                     /gene="nrdB"
                     /locus_tag="ACFM75_000734"
     CDS             complement(11068..12201)
                     /gene="nrdB"
                     /locus_tag="ACFM75_000734"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC6255317.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(12415..13395)
                     /locus_tag="ACFM75_000735"
     CDS             complement(12415..13395)
                     /locus_tag="ACFM75_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC6255318.1"
                     /translation="MTAARAGLVRFNYLKVKQAVQSKQIKGFKLWKSYDEQLEILRSR
                     GLQIDDEKKALGYLRTIGYYRLSGYFYSFRQFAPESSKSRLDEFITDTRFEDVKSLYM
                     FDKKLRQLALDALERIEVALRVNIAHTLGRHDPLAYKDSKYSATGFDHAEWLDRYNRL
                     VEREKKTGFVKHHLEHYADLPVWVACEVWDFGTMSMLYKGMLNKDKDSIAKLYRLKDG
                     KHLQTHLHAFNFIRNVSAHHSRLWNRDITFKASLKGLSDAEWKSLPINKPFVYFCLMK
                     RMLDVICPRTEWGNRLLALLDEFPDVANEAVSLRDMGLENNPRDWRLWQG"
     gene            complement(13361..15640)
                     /gene="nrdA"
                     /locus_tag="ACFM75_000736"
     CDS             complement(13361..15640)
                     /gene="nrdA"
                     /locus_tag="ACFM75_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC6255319.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYIRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCNEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            16169..16480
                     /locus_tag="ACFM75_000737"
     CDS             16169..16480
                     /locus_tag="ACFM75_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC6255320.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYKEQKTQCRAAYEAFDDFDGGRFR
                     R"
BASE COUNT         4001 a         4567 c         4089 g         3837 t
ORIGIN      
        1 tctagtacag ccccttttaa aaaacattac aatcccgagc cgccaagcga attggcaatg
       61 tccctgaccg ccgttacata catccggctg tggttgtact gccataccgt ataaaaattg
      121 ttcaagccca aataatattc aaacacgccc ggtgcggttt ccagtttgaa caaaaccgcc
      181 ttttcatcat ctgcaagctc ttcgccgggg atgatgccgt acgcctttaa atccgccacc
      241 gtccgcgtca gggcggtttt ttcgccaatg attgcctgaa catccgcacc cggcgccaat
      301 gttgcagaca ccagtatttt cccgcccgtg cgccaaccgt gctgcttcat ataattggca
      361 accgatgccg cgacatcgcc gatgttgccc catatgtccc gatgtccgtc cccgtcataa
      421 tccaccgccc atttccggta gctcgaaggc ataaattgcg gcatccccat tgcgcccgca
      481 tagctgcctt taaaggcgaa aacatcgccg ccttcttctt ttgccagctt taaaagctcg
      541 accaattctt tttggaaaaa cccggcgcgg cgggggtaat caaagcctaa ggtcgccaat
      601 gcgtccgcca cacggaaact gcccgtattt ttgccgtaat tcgtttcaat cccgataacc
      661 gccacgataa gttcggcagg cacgccgtat ttttgcgcca catcatcgat aagcgcacgg
      721 ttttccgcat aaaaccggcg cgcgccgcga aatttcgcct tgcccgaatt tcccgtgcgg
      781 aacacatacc acggacgcga tgtggagggg cggtgcataa tcttgacgat gtccgccttg
      841 taagccgctt tgtcaaaaaa atcctgccat tccgcccggg aaaaatcccc tttcccgact
      901 tcatcgtcca caaaacggcg gacatttgca ttggcggcaa acccgctgtc ggataccggt
      961 acggctgccg cgtcaaatac ggctgccgcg tcaaacgcgg ggcggctttc ttttttcatt
     1021 tcatccgcgc ggggggcttg ggcttcattt gcccggggcg ggcgtgcctc catcgccgta
     1081 caggcagaca aagccgccaa acaaattgcc agcggcagta tttttctctt tttcataaat
     1141 atgttccgaa caaatagggt aagtgggaaa gcggcgcaag ggcgcggcgc gaaatgcggt
     1201 atccgccgca atcggcggct ttgcagaatg ccgcgcgttg ccttttgcgc cgcccgaaat
     1261 ctgcctgccg ccgccgaaaa tgccgtctaa aggcacttcc cctttcagac ggcattgcct
     1321 gccgccgtat ttgcccgcta tccgcaatat cggcagtcca atatgtcttt gcggatgtcg
     1381 ttcagcaggc aggctgccgc gtcttcgtgt ttcgcctgta cgaagacaaa gagtcgggcg
     1441 atgattttgc cgataaagac tttttgcagg gcgcaggcat cggtcagggt gtgttcctgc
     1501 aggaagctgc ggatgtcttc aggcaaagtg tagtcgcggg cgacggcggc aaagcgggca
     1561 tcggtttgca ggcggtcgag cagggtttgg tttttgtcca acttcatgcc tgcttctttt
     1621 tcttcggcgg tggcgcggac aaactggtcg taaatttcgg cataaagcat atcgttcggg
     1681 ccttcaaaaa tcgtgaaggg acggatgtcg atggcgatat tgccggcggt gtgtccgcgt
     1741 tcaaaaccct tcgcgcccaa gagtttttgc aacatttgcg cggcggcata agtgtattcc
     1801 gtggcgaggg ttttgacgat gttcgcctcc atcagctgat gggcgacggg ggcaacaggc
     1861 gaaacggaat ggcagacgta gcggtaaaga atctcggaaa cctgatggcg gcgccggatt
     1921 tcgcggcgtt cgtaatcgac gaatctgatg tcgttgcgga cgtatcgtcc caggttttca
     1981 aggatgtatt ccataatgcc gtgcgtcatg ccgatcagtt gcaggcggct gcggataaag
     2041 atgttttgga acgcgcgcaa accggcagcg tcgctctggg agagtttcat cacggcggtt
     2101 gcaggcattt cggcatcgat gcggttgacg gcgtaacgga cggcgcgcaa gccttcggat
     2161 gcgagggttt cgcagcggat gtatgttttg gggacgagca gcaggtcgat gactttggcg
     2221 agtttgccgt ttttgcgctc tttggcggca acgaggagga agtcgctttg cgagttgccc
     2281 tgccagtatt tcgcggcgtt gacgtaaatg gtttgtccgt cggtatattc gtagtaagac
     2341 tgcatttcgc gtgcaatcgc cgcgccggag gtttcgggtt cggtaacgcc caaaccgccg
     2401 ccctcgcctt tgaaaatcat ctccaaacct tgcgcgactt gcgcttcatc gccgaactct
     2461 tgcagtggct gcaacaccag cgcgccttcg atgccggtac gcagcgtaac gggcacgccg
     2521 taatgccccg caatccgcaa gacttcctga atttcaaact ggctgccctt gcgcccgccg
     2581 tgttttttgt cgaggaaggg caacagcaaa cccgcctgct tcaaggcaag ccatttgtct
     2641 tcgggcaggt atcgcatcag gtcgataccg tctgaaaaaa tgcggcggaa tgcggattcg
     2701 atgtgcttta aaaaagcagc cgtgtccata gttgacggct gcgcgctcgg ttcggtgtgt
     2761 atcatcggct tcctctgtcg gttcccatta atcggcggcc ggtcaaaccg cctgccacag
     2821 tttagagttg attttctaaa ctttaccaca aagtgcgccg ggcaacaatc cgccgacctt
     2881 tcagacggca tcgcgccccc tcccgtgcta aaatgaccgt ttgcatcact gtccgccgat
     2941 tgccgcacta tgacctaccc catccccaaa ccccgtgaaa aatcccgttg gtccaatctt
     3001 tcgcaaggct cgctgccctt ggctttggcg cgttatctgc cgcacaagcg gctcaaggtc
     3061 gtgctgaccc aagatgcgga acaggcgcta cgccttcaga cggcatggcg gtttttccgt
     3121 ccgcacgaca cggcggtgtt cctgccggac tgggaaacgc tgccttacga gcgtttttcg
     3181 ccgcatcagg atttggtgtc ggaacggctg tcggcgttgt ggcagattaa gagcggcgcg
     3241 gcggacgtgt tgttcgtgcc ggttgccacg gcgatgcaga agctgccgcc cgtgccgttt
     3301 ctggcagggc gcacgttttg gctgaaaaca gggcagactt tggatatagg ccgtctgaaa
     3361 accgatttgg tagatgcggg ctacaaccat gtttcccatg ttgttgcggc gggcgagttt
     3421 gccgtgcgcg gcggcattgt cgatttgttc cccatgggca gcgaaatgcc ctatcgcatc
     3481 gatttgtttg acgatgaaat cgacagcatc aaaacctttg atacggacac gcagcgcacc
     3541 atctcccccg tttccgaaat ccgcctgctg ccggcgcacg agttccccac cgacagcgaa
     3601 gcgcaaaaaa tcttccgcag ccgcttccga gaagaagtcg acggtaatcc gaacgatgcg
     3661 gctgtgtaca aagccgtcag caacggccac ttcggcgcgg gcgtggaata ttatctgccg
     3721 ctgttttttg aaaatgagct ggaaacgctg tttgactata tcggcgaaga cgcgctgttt
     3781 gtctctttgg gcgatgttca cgccgaggca aaccgctttt ggagcgatgt caaatcccgt
     3841 tacgcgatgg cgcagggcga cgaaacctat ccgcctttgc ttccacagca tttgtatctc
     3901 tctgctgatg tttttgcagg ccgtctgaaa aactacggac aagtgctgcc cgatgtttcc
     3961 ggcaaggaac acaccctgcc cgaccttgcc gtcaaccgcc aatccgacga gccgttgcag
     4021 gcattgaagg attttcagac gacctttgac gggcggattt tgctgtgcgc cgaaagcttg
     4081 ggacggcgcg aaaccatgct cggtttcttg cagcaaaacg gtttgaaagc caaacctgtg
     4141 tccgactggc agggcttctt gtcggcgcat gagccgctga tgattacagt ggcgccgttg
     4201 gcttacgggt ttaaactggg cggactgcaa tcgccgaacc aacagcaaac tacttccgcc
     4261 tccgaaggag aaggcgatgc ggttaccgac caaaccgaat tttccgcatc cgcaacaaac
     4321 cctctcccta gccctctccc acaggagagg gaacaaagtg cagccgccgt ttcagacagt
     4381 ctgaaagcag ccgccgtttc aaccgaaagc agcctgcccc ccggtaaaag taatctgcac
     4441 gggcaaatcc aacagcaacc tgccccctcc cccgtggggg agggttgggg agagggcaaa
     4501 gcagttgccg ctcaaaccga atttcccgca tccgcaacaa accctctccc tagccctctc
     4561 ccacaggaga gggaacaaag tgcagccgcc gtttcagacg acctgaaagc caaaagcagc
     4621 ctgcatctcg tcgcaagtaa tctgcacggg caaatccgac agcaacctac tccctccccc
     4681 gtgggggagg gttggggaga gggcaaagca gtttccgctc aaactgaatt ttccgcagcc
     4741 gcaataagcc ctctccctag ccctctcccg caggagaggg aacaaagtgc cgccgccgtt
     4801 tcagacgacc tgaaaaccaa aagcagcctg catcccgtcg caagtaatct gcacgggcaa
     4861 atccgacagc aacctactcc ctccaccgtg ggggagggtt ggggagaggg caaagcagtt
     4921 gccgctcaaa gtgccatctc cgtcatcacc gaatccgatc tctaccaata cgtcgcccgt
     4981 tcgcgcgtcc acaaccgccg caagaaacac gccgccgttt cagacggtct gttgcgcgac
     5041 cttgccgaaa tcaacatcgg cgaccccgtc gttcacgaag aacacggcat cgggcggtat
     5101 atgggcttgg taacgataga tttgggcgtc gaaaccaacg aaatgatgtt gctcgaatac
     5161 gcaggagaag cgcagcttta tgtgcctgtt tcgcaactgc atttaatcag ccgctactcc
     5221 ggtcaggcgc atgaaaacat tgccctgcac aagctcggca gtggcgcgtg gaacaaggcg
     5281 aagcgcaaag ccgccgaaaa ggcgcgcgat accgccgccg agttgcttaa cctttacgcc
     5341 cgacgtgccg cccaatcggg acacaagttt gaaatcaacg agttggatta tcaggcgttt
     5401 gccgacggct tcggctacga agaaaccgaa gaccaggcag ccgccatcgc cgccgtgatt
     5461 aaagatttga cgcaggcgaa gccgatggac cgcctcgtgt gcggcgatgt cggtttcggc
     5521 aaaaccgaag tcgccctgcg cgccgcgttt gtggcggtaa tgggtggcaa acaagttgcc
     5581 gtactcgcac cgaccacgct tctggtcgag cagcacgcgc aaaattttgc cgaccgcttc
     5641 gccgatttcc ccgtgaaagt cgccagcctt tcgcgtttca acaacagcaa agccaccaaa
     5701 gccgcgctgg aaggcatggc ggacggcacg gtcgatattg ttatcggtac gcacaaattg
     5761 gtgcaagacg acatcaaatt taaaaactta ggtttagtga ttatcgatga agaacaccgc
     5821 tttggcgttc gccaaaaaga gcagctcaaa cgcctgcgcg ccaatgtcga tatccttacc
     5881 atgaccgcca cgccgattcc gcgcaccctc agcatggcac tcgaaggcct gcgcgacttc
     5941 tcgctgatta ccacagcccc cagccgccgc cttgccgtca aaacttttgt caaacccttc
     6001 agcgaaggca gcgtgcgcga agccgtgttg cgcgaactca aacgcggcgg gcaggtattt
     6061 ttcctgcaca atgaagtgga tacgattgag aatatgcgcg agcggctgga aaccctgctg
     6121 cccgaagccc gcatcggcgt agcgcacgga caactgcgcg agcgcgagct ggaacaagtg
     6181 atgcgcgact ttttgcagca aagatttaac gtgttgctct gctccaccat catcgaaacc
     6241 ggcatcgaca tccccaacgc caacaccatc atcatcaacc gcgccgacaa attcgggctg
     6301 gcgcaactgc accagcttcg cgggcgcgtc ggccgcagcc accatcaagc ctacgcctac
     6361 ctgctcacgc ccgaatacat cactaaagac gcagaaaaac gcctcgatgc cattgcggcg
     6421 gcagacgaac tcggcgcagg ttttacccta gccatgcagg atttggaaat ccgtggtgca
     6481 ggcgaaatcc ttggcgaagg acaatccggc gaaatgatac aggtcggctt cacgctctac
     6541 accgaaatgc tcaaacaagc cgtgcgcgac ctcaaaaaag gccgccagcc cgacctcgac
     6601 gcaccgttgg gcatcaccac cgaaatcaaa ctgcacagcc ccgccctgct gcccgaaagc
     6661 tactgccccg acatccacga acggctcgtc ctctacaaac gcctcgccgt ctgcgaaacc
     6721 gtgcaacaaa tcaacgccat acacgaagaa ctcgtcgacc gcttcggcct gcccgaacaa
     6781 gccgtcaaaa cccttatcga aagccaccac ttacggctta tggcaaaaga attgggtatc
     6841 gatgccattg atgcggccgg cgaagcggta acggtaacct ttggtaaaaa caataatgtc
     6901 gatccaaccg aaatcatcct gctgattcag aacgacaaaa aataccgcct tgccggcgcc
     6961 gataagctgc ggtttaccgc agagatggaa aatatcgagg tcagaatcaa caccgtaaaa
     7021 aacgttttaa aaaccttgca aaacagatgc ctgcccaaat aaagccgaca ccgcaatgcc
     7081 gtctgaaaca ccgttttcct tgtccgaaag ccgccattat gaatttgaag gaaactccac
     7141 tataatacgg cattcagatt tccagacggc atcgcgcccg tcaaaccgca cacaaaccaa
     7201 aaggaaatac atgttccgta ctatgcttgg cggaaaaatc caccgcgcca ccgttaccga
     7261 agccgattta aactatgtcg gcagtattac cgtcgatcaa gatctgttag acgcggcagg
     7321 catctacccc aacgaaaaag tcgccatcgt caacaacaac aacggcgaac gttttgaaac
     7381 ctataccatt gcagggaaac gcggcagcgg cgtgatttgt ctgaacggtg ctgcggcaag
     7441 gctggtgcag aaaggcgata tcgtcatcat catgtcttac gtccaactct ccgaacccga
     7501 aatcgccgca cacgaaccca aagtcgtctt ggtagacgga aacaacaaaa tccgcgacat
     7561 catctcctat gagccgccgc acaccgtgct gtaaccctga aaacggacat tgaccatgga
     7621 tattaaaatc aacggcatta ccgtcggtaa cgacgctcct tttgtactgt tcggcggcat
     7681 caacgtttta gaagatttgg attccaccct ccaaacctgt gcgcattacg tcgaagttac
     7741 ccgaaaactc ggtattccct atatctttaa agcctctttc gacaaggcaa accgttcctc
     7801 catccattct tatcgcggcg taggcttgga agaaggctta aagatttttg aaaaagtcaa
     7861 agcagagttc ggcatccccg tcattaccga cgtacacgaa ccccatcagt gccaacccgt
     7921 cgccgaagtg tgcgatgtca tccagcttcc cgcattcctt gcgcggcaga ccgatttggt
     7981 ggccgcaatg gcggaaacgg gcaatgtcat caacatcaaa aaaccccagt tcctcagccc
     8041 ttcgcaaatg aaaaacatcg tggaaaaatt ccgcgaagcc ggcaacggga aactgatttt
     8101 atgcgaacgc ggcagcagct tcggctacga caacctcgtt gtcgatatgc tcggtttcgg
     8161 cgtgatgaaa caaacctgcg gcaacctgcc ggttattttc gacgttaccc attccctgca
     8221 aacccgcgat gccggttctg ccgcatccgg cggtcgtcgc gcacaggctt tggatttggc
     8281 acttgcaggc atggcaaccc gccttgccgg cctgttcctc gaatcgcacc ccgatccgaa
     8341 actggcaaaa tgcgacggcc ccagcgcgct gccgctgcac cttttagaag attttttaat
     8401 ccgcatcaaa gcattggacg atttaatcaa atcacaaccg attttaacaa tcgagtaaca
     8461 cggtttcgcc ttatgatgca gactttccga aaaatcagcc tgtatgccgc aaccttgtgg
     8521 ctcggtatgc agattatggc gggttatatc gtcgcgccgg tgctgttcaa aatgctgccc
     8581 aaaatgcagg cgggcgaaat tgccagcgta ttgttcgaca tcctctcttg gagcgggctt
     8641 gccgtttggg gcgcggtact ggctgccgcc tttgccgccc taacccggcg gcaaaccgcc
     8701 ctgctgcttt ttttattgtc cgcccttgcc gccaaccgat tcttgattac acccgttatc
     8761 gaggcgctga aatacgggca tgagaattgg ctgttgtcgt ttgtaggcgg atccttcgga
     8821 atgtggcacg gcatttccag tattgttttt atggcaaccg ccctactttc agcagtttta
     8881 agttggcagc tttccggcaa agaggccgtc tgaagccctc ccatttttta cctcccttca
     8941 cttcacttgg agaacattca tgagcgcaat cgttgatatt ttcgcccgcg aaattttgga
     9001 ctcacgcggc aaccccacag tcgagtgtga tgtattgctc gaatctggcg taatgggacg
     9061 cgcagccgta ccgagcggcg cgtccaccgg tcaaaaagag gctttggaac ttcgcgacgg
     9121 cgacaaatcc cgttattcgg gcaagggcgt attgaaggcg gtcgaacacg tcaacaacca
     9181 aatcgcccaa gccctcattg gtatcgatgc caacgagcaa tcttatatcg accaaatcat
     9241 gatcgaattg gacggtactg aaaacaaagg caatttgggt gcgaatgcga ctttggcggt
     9301 ttctatggcg gttgcacgcg ccgctgccga agactcaggc ctgccgcttt accgctactt
     9361 gggcggcgca ggcccgatgt ccctgcccgt accgatgatg aacgtcatca acggcggcga
     9421 acacgccaac aacagcctga acatccaaga gtttatgatt atgcccgtcg gcgcaaaatc
     9481 tttccgcgaa gcgttgcgct gcggtgcgga aattttccac gccttgaaaa aactgtgcga
     9541 cagcaaaggc ttcccgacca cagtcggcga cgaaggcggt ttcgccccca acctgaacag
     9601 ccacaaagaa gccctgcaac tgatggtcga ggcgaccgaa gccgccggct acaaagcggg
     9661 cgaagacgta ttattcgcat tggactgcgc ctccagcgag ttctacaaag acggcaaata
     9721 ccacttggaa gccgaaggcc gctcctacac caacgcggaa tttgccgaat atctggaaag
     9781 tctggtcaac gagttcccca tcatctccat cgaagacggc atggatgaaa acgactggga
     9841 aggctggaaa ctgctgaccg aaaaactggg cggcaaagtc cagctcgttg gcgacgacct
     9901 cttcgttacc aatccgaaaa tccttgccga aggcattgaa aaaggcgtgg caaacgcact
     9961 attggtcaaa gtcaaccaaa tcggtacttt gagcgaaacc ctgaaagccg tcgatctggc
    10021 aaaacgcaac cgctacgcca gcgtgatgag ccaccgctcc ggcgaaaccg aagacagcac
    10081 cattgccgac ttggcagtcg ccaccaactg tatgcagatt aaaaccggtt ccctgtcccg
    10141 ttccgaccgt atggcgaaat acaaccaact gctgcgtatc gaggaagaat tggcggaagc
    10201 cgcctactac cccggcaaag ccgcattcta ccaactgggc aaataagaaa aggttaaggt
    10261 atgaagtggg taactgtcgt tttatccttc gcacttgtct gttgccaata cagcctctgg
    10321 ttcggcaaag gcagtatcgg gcgcaacaac aacctgaaaa atcagattgc cgttcaggaa
    10381 gaaaaaaacc agacactcgc cctacgcaac cattcccttg cagccgaagt ctatgatttg
    10441 gaaaacggtc aagaagccat ttcggaaatc gcccgggtag aactgggtta tatccaagac
    10501 ggtgaaacct tttaccgact catcaggcat aaccggtaat accgtcaaaa agccgtccga
    10561 accaatgttc ggacggcttt tatttcaaca aactgtcaga cagcccctca tcctcccccg
    10621 acaaaccgca atccagcctg acatccccct cgacgcaaca gcagcacggc agtatctcgt
    10681 cccgccccaa aaaagccaaa ggcggctccc gataagtaac gcttccctcc aaaatcttca
    10741 ctcggcacga tccgcaatat ccgcttcggc actgatattc caccatatgc cccgtccgtt
    10801 ccaagccttc caacagggtc tcgccctcca agagctcaaa aaaaccctta ttcgtaccaa
    10861 tgcgcgccat ttccgaccaa tcaaaaatat agtggattaa atttaaacca gtacggcgtt
    10921 gcctcgcctt gtccggattt ttgttaatcc actataatcc actataaaag gaacaataac
    10981 cgatcctacc cgctgttttt cccatcatac aacatacaaa tgccgtctga aacatccggc
    11041 ttcagacggc attttttcaa aaaacattta caactcaaaa tcgcccaaat catccgtatt
    11101 cacttcagaa tctatctgac cgatcaaata agaggatatt tccacttcct gcggcgcgac
    11161 ctgtacgttg tcggacgaca gccacgcatt aatccacgga atcgggtttt gatttgcgcc
    11221 ttcaaatccg gccggcagcc ccaccgcctg catacgcaga ttggtaatat attcgacgta
    11281 ttgggataag atttctttgt tcaaaccgat catcgaaccg tccttaaaca aatatgccgc
    11341 ccactctttt tcctgttccg ccgctttttt gaagagttgg aaacattcgt cctgcaactc
    11401 ggcggcaatt tctgccattt cagaatcatc gacacccgaa cgcatcagat taagcatatg
    11461 ctgcgtgccg gtcaggtgca gggcttcgtc gcgggcaatc agtttgatga ttttggcgtt
    11521 gccttccatc aactcgcgct cggcaaaagc aaacgagcag gcgaatgaaa cgtagaaacg
    11581 gatggcttcc aacacattga cgcacatcag gcagagatag agttttttct tcaacccgcg
    11641 caaagacacg gtaacgggtt tgccgccgac attgtgcacc ccttcgccca acaggttgta
    11701 atactgggtg tattcgatta agtcatcgta atagcaggca atgtcttcgg cgcgggcggt
    11761 aatgtattcg ttttcgacaa tatcatcaaa cacgaccgac ggatcattca caatattgcg
    11821 gatgatgtgg gtatagctgc gcgagtggat ggtttcgctg aagctccacg tttcaatcca
    11881 cgtttccaac tcgggaatcg aaaccaaagg cagcaaagca acattcggac tgcgcccttg
    11941 gatggaatcg agcagtgttt ggtatttcag attgctgatg aaaatatgtt tttcgtgttc
    12001 tggcaggttg gcgtagtcga tacggtcgcg cgacacgtcg atttcttccg gccgccaaaa
    12061 aaacgacaat tgtttttcaa tcagtttttc aaatacctcg tatttctgct ggtcataacg
    12121 ggcgacatta accggctgac cgaaaaacat cggctcattc agcgcgtcgt ttttggtttt
    12181 gggaaaggtg ctgtatgaca tgactaggtg ttcgcaggac atggttttct caggtttatt
    12241 taaatttagg aatttggatt tcagacagcc ttatatgttt taagtaaggt cgtctgaatg
    12301 tatctgtaca ttgaattctt gccttaaata tcgctgttag ttataatctt ccccaacaac
    12361 tcccccaact ttgccaacct acgcggttgg agcaacaagg ttgggggctt tcttttatcc
    12421 ctgccacaat ctccagtctc gcggattatt ttccaaaccc atatcgcgca aacttacagc
    12481 ttcattggcg acatcgggaa actcatccag taaagcaaga agacggtttc cccattcggt
    12541 tcgcggacaa ataacatcca acatccgttt catcaggcaa aaatacacaa acggtttatt
    12601 gataggcaat gatttccatt ctgcatcaga caaacctttc aagctggctt taaaagtaat
    12661 atccctattc cataatcgac tgtgatgtgc cgaaacgttg cggataaagt taaatgcgtg
    12721 cagatgtgtt tgcagatgct taccgtcttt caaacgatac aatttggcaa tagagtcttt
    12781 atctttattc aacatccctt tatacagcat agacatcgtg ccgaaatccc atacttcgca
    12841 ggctacccaa acaggtaagt cggcataatg ttccaaatga tgtttaacaa aaccggtttt
    12901 cttttcgcgc tcgaccagcc tgttatacct atccagccat tccgcatggt cgaaacctgt
    12961 ggcagaatat ttgctatctt tgtaagccaa tgggtcatgc cgtcctaatg tatgggcaat
    13021 atttacccga agcgctactt caattctttc cagtgcatct aatgccaatt gccgcaattt
    13081 cttatcgaac atataaaggc ttttgacatc ttcaaaacga gtatctgtaa taaactcatc
    13141 caacctgctc ttagaacttt caggagcaaa ttgtcggaaa gagtaaaaat aaccgcttaa
    13201 tcggtaataa ccaatggtgc gtaaatatcc taatgccttt ttctcatcat caatctgcaa
    13261 tcctctactt cttagaatct ccaactgctc atcgtaagat ttccaaagct tgaagccttt
    13321 tatctgcttg ctttgcacag cctgtttcac tttcaaatag ttaaatctta caagccccgc
    13381 ccgcgcagcc gtcatcctga atatcggtct gcgtatcgtc cgcgccgtcg cgggtgttat
    13441 ggtagtacag ggttttgacg ccgtatttgt aggcggtcag caggtctttg agcatttgtt
    13501 tcatagaaac tttgccgcct tcgaatttgc ccgggtcgta ggcggtgttg gcggaaatcg
    13561 cctgatcgac gaatttttgc atcacgccaa caagtttcag atagccttcg ttgccgggaa
    13621 gctgccacag ggtttcatag gcatttttca gggtttcaaa ctccggcacg acttgtttca
    13681 aaatgccgtc tttcgatgct tttaccgtta ccaatccgcg cggcggctcg ataccgttgg
    13741 tggcgttggc gatttgcgaa ctggtttcag acggcatgag tgcggttaga gtagagttac
    13801 gcaggccgta tttgacgatg tcggcacgca ggctttccca atcgtagtgc aaaggctcgt
    13861 tacacacagc gtccaagtct tttttgtaag tgtcgatggg cagtttgcct tgcgaataaa
    13921 cggtttggtt aaagagcgta cacgcaccgt attctttggc aaggtttgcc gatgctttga
    13981 gcagataata ctgtatggct tcaaaggtac ggtgggtcag accgagcgcg gagccgtcgc
    14041 tgtagcggac accgtttttc gccagataat aagcatagtt gatgacgccg atgccgagcg
    14101 aacggcgacc catagtagag gtacgcgcgg cttctaccgg atatccctga taatctaaaa
    14161 gtgcatcgag cgcgcgaacg gtcaagtcgg caagcccttc caattcgtcc aagctgttta
    14221 atgcgcccaa gttaaaggca gacagtgtac acaaggcgat ttcgccgttc ggatcgttga
    14281 tattgtccag cggtttggtc ggcagagcga tttccataca caagttggac tgatgaacag
    14341 gcgcgacgcg cggatcgaac gggctgtgcg tattgcagtg atcgacgttt tgaatgtaga
    14401 tgcgcccggt tccggcacgc tcctgcatca gcgtggaaaa caggtcggca gccggaatga
    14461 tgcgcttgcg gatatcaggg tcttgctcgt atttcgtata gagccgctca aattcgtctt
    14521 ggtcggcaaa aaacgcttcg tacaatcccg gaacctcgtt gggcgaaaac agcgtaatgt
    14581 tgccgccctt aatcaggcgg atgtacagca ggcggttgat ttgcacgccg taatcaagct
    14641 gacggatacg gttgtcttcc acaccgcggt tgtttttcaa caccagcagg ctttcggctt
    14701 cgatatgcca caaggggtag aacaaagttg ccgctccgcc gcgcacgcca ccttgcgaac
    14761 aggatttgac tgccgcctga aacattttaa agaagggaat gcagccggta tgccgtgctt
    14821 cgccgccccg gatttcgctg tccagtccgc ggatacgtcc ggcattgatg ccgatgcccg
    14881 cacgctggga aacgtatttc acaatcgcgc tggtggtggc gttgatggaa tccaggctgt
    14941 cgtcgcattc gatcaatacg cagcttgaga actggcgcgt aggcgtacgc acgccgctca
    15001 taatcggggt cggcagcgat actttaaatg tggaaacggc atcgtaaaac cgtttgacgt
    15061 aatcgaggcg tgtttctttg ggatatttgc tgaaaagaca catcgccacc agaacatata
    15121 agaactgcgg tgtttcataa atctggcggg taacgcggtt ctgtaccaga tatttgcctt
    15181 cgagctgttt gacagcggca taggaaaagg acatatcgcg ttcgtggtcg atataggcgt
    15241 tcagttcatc aaattcttcg cggctgtaat cctcaaggat atgcctgtcg tattttccgg
    15301 catcggtgag ctttttaacg tggtcgtaaa ggtgcggcgg ctcgtattcg ccgtaggcta
    15361 ttttacgcag atggaaaatc gccaaacgcg cggcaaggta ttggtagtcc ggagtatctt
    15421 ccgaaattaa atcggcagcg gccttgatga tggtttcgtg gatgtcgtcg gtgcggatgc
    15481 cgttgtagaa ctggatgtgc gatttcaact cgacctgcga cacggaaaca ttttccaatc
    15541 cttccgccgc ccaagtaacg acacggtgaa tcttatccaa atcaatggct tctaatcttc
    15601 cgtctcgttt ggttactttc aaatcagtcg gtgtattcat cgcttcctct tccactcttg
    15661 atattcaaga cacggtcttt tcaaataaat taagacagac aatatagtgg atttttggca
    15721 ttttctccag tcttgacaac ggttgcattt ttcagtttca ggcaatgcgc gataaaaccc
    15781 gcctgtcgta tttttcctat aatatttgtt ttatctgata attctttacc gataaaaaac
    15841 gggtaaattt tttgcctttt gaccggctcc ggctacaagg cggtgaaata aggattttcc
    15901 gacgaaaaag gaaagcttcc ggttttctgc ccctgcgaat tgttaaattt tgcaaagtat
    15961 gattttgccg cgccgccgcc gacaaattcc attttcttac cgattgaaat ttattattga
    16021 gattaatgtg ttatttgaat ccgcatatca aacggcaagt tttgtcggct gaatgagtct
    16081 gaaactgccg acaatttgcc cgttccatcc ttccatccta tactggaagg aatgacaaac
    16141 tgaaaggatc gtcatatctg tcaaaaagat gcttgccata ctgttgtctg caatattggg
    16201 actggtatcg acaactgccg ctgccggtac gtcagaaccc gcccaccgcc ataccaaaca
    16261 tatccgcaag gcaaacaagc agatgctgca ccccgaatgc aggaaatatt tggaacgccg
    16321 tgccgcgtgg taccgatcgc aaggcaacgt gcaggaattg cgcgaaaaca aaaaggcgcg
    16381 caaagcattc cgctccctgc cttataagga acagaaaacc caatgccggg cggcttatga
    16441 ggctttcgat gatttcgacg gcggcaggtt ccgccgttaa tcccatataa aata
//
LOCUS       DAXHRI010000030        16457 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXHRI010000030 DAXHRI010000000
VERSION     DAXHRI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.030"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            209..1273
                     /locus_tag="ACFM75_000738"
     CDS             209..1273
                     /locus_tag="ACFM75_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC6255321.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVNDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="ACFM75_000739"
     CDS             1414..1737
                     /locus_tag="ACFM75_000739"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC6255322.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="ACFM75_000740"
     CDS             1809..2132
                     /locus_tag="ACFM75_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC6255323.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="ACFM75_000741"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="ACFM75_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC6255324.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="ACFM75_000742"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="ACFM75_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC6255325.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRPKHR
                     AKPKLRVRKS"
     gene            3087..3857
                     /gene="tatC"
                     /locus_tag="ACFM75_000743"
     CDS             3087..3857
                     /gene="tatC"
                     /locus_tag="ACFM75_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC6255326.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIANPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3910..4515
                     /locus_tag="ACFM75_000744"
     CDS             3910..4515
                     /locus_tag="ACFM75_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6255327.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPANIDETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSAGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIRYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4531..4884
                     /locus_tag="ACFM75_000745"
     CDS             4531..4884
                     /locus_tag="ACFM75_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC6255328.1"
                     /translation="MNRQTTYLLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4874..6688
                     /locus_tag="ACFM75_000746"
     CDS             4874..6688
                     /locus_tag="ACFM75_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC6255329.1"
                     /translation="MPAETTVSGAHPTAKLPIYILPCFLWIGIVPFTFALRLQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFTGWEDTPL
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVLLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6878..7555
                     /gene="misR"
                     /locus_tag="ACFM75_000747"
     CDS             6878..7555
                     /gene="misR"
                     /locus_tag="ACFM75_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC6255330.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7569..8975
                     /gene="misS"
                     /locus_tag="ACFM75_000748"
     CDS             7569..8975
                     /gene="misS"
                     /locus_tag="ACFM75_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC6255331.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9093..11519
                     /locus_tag="ACFM75_000749"
     CDS             9093..11519
                     /locus_tag="ACFM75_000749"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6255332.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDNLSTAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EENQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQSAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11564..11809
                     /gene="rpsP"
                     /locus_tag="ACFM75_000750"
     CDS             11564..11809
                     /gene="rpsP"
                     /locus_tag="ACFM75_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC6255333.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11825..12334
                     /gene="rimM"
                     /locus_tag="ACFM75_000751"
     CDS             11825..12334
                     /gene="rimM"
                     /locus_tag="ACFM75_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC6255334.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTVSVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12334..13083
                     /gene="trmD"
                     /locus_tag="ACFM75_000752"
     CDS             12334..13083
                     /gene="trmD"
                     /locus_tag="ACFM75_000752"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC6255335.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13099..13464
                     /gene="rplS"
                     /locus_tag="ACFM75_000753"
     CDS             13099..13464
                     /gene="rplS"
                     /locus_tag="ACFM75_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC6255336.1"
                     /translation="MNLIQQLEQEEIARLNKDIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13785..14510
                     /locus_tag="ACFM75_000754"
     CDS             13785..14510
                     /locus_tag="ACFM75_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC6255337.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14534..15409
                     /locus_tag="ACFM75_000755"
     CDS             14534..15409
                     /locus_tag="ACFM75_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC6255338.1"
                     /translation="MNLYDLLLAPFTEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15486..16412
                     /locus_tag="ACFM75_000756"
     CDS             15486..16412
                     /locus_tag="ACFM75_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFC6255339.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQNLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALI
                     KADPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHI
                     EFIAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSG
                     KLYSDALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4276 a         5281 c         3623 g         3277 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtca cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg aacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagtaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcaacg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cggacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac tcaaatcgaa ctggaagaac tgaggaaggc aaagcaggaa
     2581 tttgaagctg ccgccgctca ggttcgagac agcctcaaag aaatcggtac ggatatggag
     2641 ggtaatctgc acgacatttc caatggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgccg atttcggtgt cgatgaaaac ggcaatcccc ttcccgatgc ggcaaacacc
     2761 ctatcagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc
     2821 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgc
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg atactgctgt tgaaacgcct gttccgcaca ccacttccct gcgcaaacag
     3001 gcaataagcc gcaaacgcgg tttgcgtccg aaacaccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagggca aatccggtgt ccgaaacaca aaacgaacaa cccgtccaac
     3121 cgcttgtaga gcatctcatc gagctgcgcc gccgcctgat gtggacggtt gtcggcatct
     3181 tagtctgctt tttcggcatg atgccgtttg cccaacaact ctatactttt atcgccaacc
     3241 cgctgatggc aaacctgccc aaagacacca gcatgattgc caccgatgtc atcgcaccgt
     3301 ttttcgtgcc ggtcaaagtt accctgatgg cggcattttt aatttcgctg ccgcatacgc
     3361 tctaccaaat ctgggcattt gtcgcccccg cactctacca aaacgaaaaa cgcctgatta
     3421 cgccgctcgt cctctccagc gtcagcctgt ttttcatcgg tatggcattt gcctactttt
     3481 tggttttccc cgtcattttc aaattccttg ccggcgttac ccctgtcggg gtcaatatgg
     3541 cgacagacat cgacaaatac ctctccttca tcttggggat gtttgtcgca ttcggtacaa
     3601 cgtttgaagt ccccattgtc gttatcctgt taaccaaaat tggtgtggta acaaccgaac
     3661 agctcaaacg cgcccgcccc tatgtgattg tcggcgcgtt tgtcattgcc gccatcatca
     3721 cgccgcccga tgtgatttca caaaccctgc ttgccattcc gctgattctc ttatacgaag
     3781 caggtatttg gttcggacgc tttttcacgc cacgttcaga acaggatggc gacatacagc
     3841 cgcctgcaac aacctgacac tatgccgtcc gaacctccgc ctcataccgc aacagattaa
     3901 ggaatacctt tgaataccct ctatttaggt tcaaacagcc cgcgccgaat ggaaatcctg
     3961 acacagttgg gctatcaggt cgtcaagctg cctgccaaca tcgacgaaac ggtcagacag
     4021 aacgaagacc ctgcccgtta cgttcaaagg atggcagaag aaaaaaaccg aaccgccctg
     4081 accctctttt gcgaaaccaa cggcacaatg cccgatttcc ccctgattac cgccgacacc
     4141 tgcgtcgttt cagccggcat tatattaggc aaaccccgct cccaagccga agcgatcgaa
     4201 tttttaaacc gattgtccgg caaacaacat accgtcctga ctgccgtctg catccgttat
     4261 cgcggcaaaa cgtcaagccg cgtccaaacc aaccgcgtcg ttttcaagcc cctgagttcg
     4321 gaagaaattt ccgcctatgt ccaaagcggc gaaccgatgg acaaagccgg cgcctacgcc
     4381 gtacaaggca taggcggcat ctttatccaa tctatcgaag gcagcttcag cggcattatg
     4441 gggctgcccg tttatgaaac cgtttcgatg ttgcaggatt tgggataccg taccccacct
     4501 ttcatccgag cctaacccgt aaagaccgcc gtgaacagac aaaccactta cctccttgcc
     4561 tctttcagcc tgatcgcact gataatcctg tccctttcct gggaactgtg gattgcaccg
     4621 ttgcgcccgg gcggttcgtg gctcgcgctc aaagccctcc ccctctgtct gccgctttca
     4681 ggtatcttga aaaagaaaat ctatacctac caatacagct ccatgctggt tctgatttat
     4741 tttgccgaag ccgttatgcg cctgttcgac gcctatccgg cagaacagat ttgcgccgcg
     4801 ctttccgcag tattcagcat catcttcttc atatcctgcc tgtcgttcgt caaacaatac
     4861 aaggaaacaa acaatgcccg ctgaaacgac cgtatccggc gcgcacccca ccgccaaact
     4921 gccgatttac atcctgccct gcttcctttg gataggcatc gtccccttta cctttgcgct
     4981 caggctgcaa ccgtcgcccg acttttacca cgatgccgcc gccgcagccg gcctgattgt
     5041 cctgttgttc ctcacggcag gaaaaaagct gtttgatgtc aaaatcccac ctatcagctt
     5101 ccttctgttt gcaatggcgg cgttttggta tcttcaggca cgcctgatga acctgattta
     5161 ccccggtatg aacgacatcg tctcttggat tttcatcttg ctcgccgtca gcgcgtgggc
     5221 ctgccggagc ttggtcgcac actacggaca agaacgcatc gttaccctgt ttgcctggtc
     5281 actgcttatc ggctccctgc ttcaatcctg tatcgtcgtc atccagttta ccggctggga
     5341 agacaccccc ctgcttcaaa acatcatcgt ttacagcggg caaggcgtaa tcggacacat
     5401 cgggcaacgc aacaacctcg gacactacct catgtggggc atactcgccg ccgcctacct
     5461 caacggacaa cgaaaaatcc ccgccgccct cggcgcaatc tgcctgatta tgcagaccgc
     5521 cgttttaggt ttggtcaatt cgcgcaccat cttgacctac atagccgcca tcgccctcat
     5581 ccttcccttc tggtatttcc gttcggacaa atccaacagg cggacgatgc tcggcatagc
     5641 cgcagccgta ctcctaaccg cgctgttcca attttccatg aacaccattc tggaaacctt
     5701 taccggcatc cgctacgaaa ctgccgtcga acgcgttgcc aacggcggtt tcacagactt
     5761 gccgcgccaa atcgaatgga ataaagccct tgccgccttc cagtccgccc cgatattcgg
     5821 gcacggctgg aacagttttg cccaacaaac cttcctcatc aatgccgaac agcacaacat
     5881 atacgacaac ctcctcagca acttgttcac ccattcccac aacatcgtcc tccaactcct
     5941 tgcagagatg ggaatcagcg gcacgcttct ggttgccgca accctgctga cgggcattgc
     6001 cgggctgctg aaacgccccc tgacccccgc atcgcttttc ctgatctgca cgcttgccgt
     6061 cagtatgtgc cacagtatgc tcgaatatcc tttgtggtat gtctatttcc tcatcccttt
     6121 cggactgatg ctcttcctgt cccccgcaga ggcttcagac ggcatcgcct tcaaaaaagc
     6181 cgccaatctc ggcatactga ccgcctccgc cgccatattc gcaggattgc tgcacttgga
     6241 ctggacatac acccggctgg ttaacgcctt ttcccccgcc actgacgaca gtgccaaaac
     6301 cctcaaccgg aaaatcaacg agttgcgcta tatttccgca aacagtccga tgctgtcctt
     6361 ttatgccgac ttctccctcg taaacttcgc cctaccggaa taccccgaaa cccagacttg
     6421 ggcggaagaa gcaaccctca aatcactaaa ataccgcccc cactccgcca cctaccgcat
     6481 cgccctctac ctgatgcggc aaggcaaagt tgcagaagca aaacaatgga tgcgggcgac
     6541 acagtcctat tacccctacc tgatgccccg atacgccgac gaaatccgca aactgcccgt
     6601 atgggcgccg ctgctacccg aactgctcaa agactgcaaa gccttcgccg ccgcgcccgg
     6661 tcatccggaa gcaaaaccct gcaaatgacc cgcgccggcg gatgcggata ccgcccgaaa
     6721 tgtaaagctc catgcaagac attgcaaaaa acagcaaacc ggtagggaaa tacgctatca
     6781 aaaaacattg cagccgtgtt aagataaacc gtcaaacaat cttttcacgc cccgcccgaa
     6841 acagggtcgg ggcataccct tacgaaaagg aaacaccatg agccgcgtat tactcgtaga
     6901 tgacgatgcc ctgctaaccg aactgctgac cgaatacctg agcgccgaag gtctgaacgt
     6961 ccgcagcgtt cccgacgggg aagcaggcgt acaggaaatc ctgagcgggc agtacgatgt
     7021 agtcgtattg gattccatga tgcccaaaat gaacggcttg gatgtcttga aaaacgtacg
     7081 cgcccgaagc accgtcccca tcatcatgct gaccgccaaa ggcgacgaca tcgaccgaat
     7141 catcggcttg gaaatgggcg cggacgacta tgtccccaaa ccctgcacac cacgcgaact
     7201 cttggcacgc atcaatgcca tcctgcgccg cgcacaacac agcggcgaac agaacaacgc
     7261 acccaacagc atctccgtca gcgatgtcgt cctgtacccc gccaaacgcc aggcatccgt
     7321 caaagacatg ccgctcgaac tgaccagcac cgaattcaac ctgctcgaag tcctgatgcg
     7381 ccatgccgga caggtagtca gcaaagaaac cctgtccgtc gaagcactcg accgcaagct
     7441 ggcaaaattc gaccgcagta tcgacgtaca catctccagc atccgccaca agttgggcga
     7501 tgcctctctg attcaaaccg tacgcggctt gggctacctg tttgtcaaaa actgaaataa
     7561 acagataaat gaaactgttc caacgcatct tcgccacatt ttgcgcggtt atcgtctgtg
     7621 caatctttgt ggcgagtttt tctttctggc tggtgcagaa cacccttgcc gaaaaccagt
     7681 tcaaccaacg ccgcaccatc gaaaccactt tgatgggcag catcatttcc gcattccggg
     7741 cacgcgggga cgcgggcgcg cgcgaaatcc tgacggaatg gaaagacagc cccgtctcat
     7801 cgggcgtgta cgttatacag ggcgacgaga aaaaagatat cctgaaccgg tatatcgaca
     7861 gctataccat cgaacgcgcc cggcttttcg ccgccggaca cccgcattcc aacctcgtcc
     7921 atatcgaata cgaccgcttc ggcgaagaat acctgttctt caccaaagac tgggacaaac
     7981 tccaagcccg ccgcctgccc agccccctgt tgatccccgg cctgccgctc gccccgattt
     8041 ggcacgaact catcatattg tccttcatca tcatcgtcgg actgctgatg gcatatatcc
     8101 tcgccggcaa cattgccaaa cccatcagaa tcttaggcaa cggcatggac agggtggcaa
     8161 acggagaact tgaaacccgt atctcccaac aggtcgacga ccgcgacgac gaattgtccc
     8221 atcttgccat ccaattcgac aaaatggtgg aaaaactcga aaaactcgtt gccaaagaac
     8281 gccacctgct ccatcacgtc tcccatgaaa tgcgttctcc ccttgcgcgc atgcaggcaa
     8341 ttgtcggact gattcaggcg cagccccaaa aacaggagca atatctcaaa cggctggaag
     8401 gcgaactgac ccgcatggat acgctggccg gggaactgtt aaccctgtcc cgtctcgaaa
     8461 cttccaatat ggctttggaa aaagaaagcc tgaaactcct gcccttcctg ggcaacctgg
     8521 tagaagacaa tcaaagcatt gcccagaaaa acggacaaac ggttaccctg tctgccgacg
     8581 gaaaaatccc cgaaaataca accatccttg ccaacgaaag ctacctgtac cgcgccttcg
     8641 acaacgtcat ccgcaacgcc gtcaactaca gtcccgaagg cagcaccatc ctgatcaaca
     8701 tcggacaaga ccacaaacac tggataatcg acgttaccga caacggccct ggcgtggacg
     8761 aaatgcagct cccgcacatc ttcaccgctt tctaccgtgc agactccagt gcgaacaaac
     8821 ccggaacagg actggggctt gcattgaccc aacatattat tgaacagcac tgcggaaaaa
     8881 tcatcgccga aaacatcaaa ccgaacggtc tgcggatgcg cttcatcctg cccaagaaaa
     8941 aaaccggttc caaaacagaa aaaagtgcga actgaccata atgccgtctg aaccggcttc
     9001 agacggcatt gcacaaacag ttaccccaaa acaaacacac agcgggtgca tctccaacaa
     9061 tcacgcacat accgtccgca aaggaatata tcatgtcggc acaaaccgat ccgggctact
     9121 ttttcatgcc caaccacatt attctgatag gcgcgagcga acgtccgtac agcctgggcg
     9181 aacgcgtact cagcaacctg ctgagtacgc cctttcaagg aaaaatcacc cccgtaaacc
     9241 cgcgccacca caccatagcc gggcttcccg cctacaccag cctcaacaaa atccccggca
     9301 atgcagacct gattattgcc gttaccctac ccgacagtta cgacaccctc ttcaaaacct
     9361 gccgcaaaaa gcagctccga cacatcatcc tcatacagga ctgggacaac ctgtctaccg
     9421 cagaactgca caccgccgaa actgccatcc gcaaacacca cggcaacgga ctcaacatca
     9481 ccgcctgcac caccgcaggc atccaactgc cctcactcgg acttaacatc agtacccaag
     9541 acggatacgc cgcaggccat accgccatac tgaccggcaa tgccgccgtc agccgccaaa
     9601 tcgacaacat cctgaacaaa ctccgtcaag gcacatcccg ccacatcagc ctgcatcccg
     9661 gcatcagccc catcacatcc gccgattggc tcaaccgctt cggacacagc ctgcacacca
     9721 aaaccgccgt cctacaccac aaccctgaag agaatcagcg caaactgttc agcgcaatcc
     9781 gccaatttac ccgccatacg ccgctgattc tccacatcac ctgccgcacg acagaaaccg
     9841 accgtgccgt actgcactgc ctcgcccgac actgcaactt cctcatcagt ttcaacgccg
     9901 acgacctcga agccgcactg cgcgcccaac tgtccgacct tccacccctg tcccgactcg
     9961 acatcctgtc cgacacgcct gccgaatggc tgaacgcgca cgcgccaaaa accctcgccc
    10021 tccactttcc caaccttccc caccacatcc gcaacggaca cctgaccggc acacccacac
    10081 cctcaatctg ccacgacatc gcctcacgtc agcttgcccg ccccgacacc caagccgtcc
    10141 tgaccatcct cagcccctcc ggacacgagg attacaaaaa aacagcacgc gcccttatcc
    10201 gcctgtccga acagagcgcc aaacccctgc tcgtcagcag ccccttttca gacggcataa
    10261 cacatttcga cacccccact caggcaatcc gcaccctttc ctaccgcaac accgccgccg
    10321 ccctgaaaca ggcacagctc gacattgcac cgccgcagcc atgccgtctg aaaacaccgc
    10381 aaccccaaaa catcaaaaaa gccctcgaag ccgccgaccc cgccctaatc gccgaagccc
    10441 tgcacctccc cccctaccgg cacactaccc ataacgccgt ccaattccaa ttcggcagcc
    10501 actccctcta tggcgacatc ctgaccgcac gttgcaacgg acaaaccact gccgtactcc
    10561 cgccgtttac cacgctcgac agccgccacc ttgcccgctt tgccgaactc gacggcacac
    10621 aaaccctcga ccagttcctg cacacactga ccatcattcc cgaataccgc caacacattc
    10681 tcggcatcac cctcaaccta aacggcggac aatacagcag cgatttcatc ttaaagacac
    10741 ccgaaacaca cgacacgccc aaacgcaaga acacaggcaa agccgcccaa accctcgaac
    10801 atgccgccgc aaaaatgcag agtgccgccg catacctgaa acacaaaaac ccgacagccg
    10861 ccgaatttct ccgccacaca agcgaagccg ccgcagaact gctcggcagc aaaaccgaaa
    10921 ccggagcagc cgtacccaac gtacttgccc cctatcccgc agcatacccc aaaacactgt
    10981 ccctaagaaa caacacgacc gttaccatta cccccatttt gcccgaagac gcagaagcca
    11041 aacagcagtt cgtccgcagc ctcggtcccg aagcacggta cacacgcttc atgacccaca
    11101 ccaacgaact gcccgcagcc acgttggcac gcctgtgcaa ccccgattac cactgtgaag
    11161 ccgcatggac ggcaaaggat gccgacagca acatcgtcgc cgtcgtccgc cacagccgcc
    11221 tgaaccgcaa cgaatgcgaa ttcggcatca cactggcgga acatatgcgc ggcagcggac
    11281 tggcacagaa aatgatggaa ctcatcatcc aaaccgccgc acagcaaggc taccggacta
    11341 tgagtgccga cattctcaaa accaataccc ccatgatcaa acttgctgaa aaatcaggat
    11401 ttaccctcaa ggaatcggac accgaaaaaa acctgtaccg cgcatatctg aaccttgcgg
    11461 cagacaaaac aacagaaaaa acaaataaaa acttgcgcac cgaccacaaa ataacctaaa
    11521 atcggcagtt tccatatatc cgattttctc aaaaggactc aaaatggtag ttatccgttt
    11581 ggcacgcggc ggctcgaaac accgcccctt ctacaacgtc atcgttactg actcacgcag
    11641 ccgccgcgac ggccgcttca tcgaacgcgt aggcttctac aaccccgtag ccaacgaaaa
    11701 acaagagcgc gtccgcctca atgcagaccg cctgaaccac tggattgcac aaggcgcgca
    11761 agtcagcgac tccgttgcaa aactgattaa agaacaaaaa gccgcctaat ccgcatttgc
    11821 cgccatgaca gacactcaaa accgggtagc catgggctac atcaaaggcg tattcggcat
    11881 aaaaggctgg ttgaaaattg ccgccaacac cgaatattcc gacagccttt tggactaccc
    11941 cgagtggcat ttggtcaagg acggcaaaac cgtcagcgtt acccttgaag ccggaaaagt
    12001 cgtcaacagc gaactccaag tcaaattcga aggcataaac gaccgcgact tggcattctc
    12061 attgcgcggt tacaccatcg aaataccccg tgaagcattc gccccgacag aagaagacga
    12121 atactactgg acagacttgg tcggcatgac cgtcgtcaac aaagaccata ccgttttagg
    12181 caaggtaagc aacctgatgg aaaccggcgc aaacgacgta ttgatgattg acggagaaca
    12241 cgggcagatt ctgattccgt tcgtttccca atatatcgaa accgtcgata ccggcagcaa
    12301 gaccattact gccgactggg gtttggacta ctgatgctta tccaggcagt taccattttc
    12361 cccgaaatgt tcgacagcat tacccgctac ggcgtaacgg gacgcgcgaa cagacaggga
    12421 atctggcagt ttgaagcagt caatccccga aagtttgccg acaacagatt gggctacatc
    12481 gacgaccgcc cgttcggcgg cggtccggga atgattatga tggctccgcc gcttcatgcg
    12541 gcaatagaac acgccaaagc acaatcttcc caaaccgcaa aagtcatcta cctcagcccc
    12601 caaggaaaac cgctgacaca ccaaaaagcg gcagaactgg cagaacttcc gcatctgatt
    12661 ctgctgtgcg gacgctatga gggaatagac gaaagactgc tgcaaaccag cgtcgatgaa
    12721 gaaatcagca tcggagactt cgttgtttcc ggcggagagc ttcccgccat gatgctgatg
    12781 gatgcggtat tgaggctcgt acccggcgta ttgggcgata tgcagtctgc cgaacaagat
    12841 tcgttctcaa gcggcatttt ggactgcccc cactacacca aacccttaga atttcaaggc
    12901 atggctgttc cggaagtatt gcgttccggc aatcatggct tgatagcgga atggcggttg
    12961 gaacaatcgc tgcgccgcac cttggagcgc agacccgatc ttttggaaaa gcgcgtttta
    13021 atcccaaagg aatcccgcct cttagaaacc atccggcaag agcaacggga aatccaatca
    13081 taatttagga aaaaaacaat gaacctgatt caacagctcg agcaagaaga aattgcccgc
    13141 ctgaacaaag acatccccga attcgcaccg ggcgacaccg tagtcgtatc cgtacgcgtc
    13201 gtggaaggta cccgcagccg tctgcaagcc tacgaaggcg tggttattgc ccgtcgcaac
    13261 cgtggtctga acagcaactt catcgtccgc aaaatctcca gcggcgaagg tgttgaacgt
    13321 actttccaac tgtactctcc gaccgtcgaa aaaatcgaag tcaaacgccg tggcgacgta
    13381 cgccgtgcca aactgtacta cctgcgcggc ctgaccggta aagctgcacg catcaaagaa
    13441 aaactgcctg cacgcaaagg ttgattcaaa ccgttttccc ccaatgccgt ctgaaccttc
    13501 agacggcatt tcttattgct gcgatgccgg caagtcaacg atatgcctgc ccctcatcca
    13561 ccttgattaa caatcactcc cgcctattca agcataattt acagaccaaa cgttatacag
    13621 tatcatatca gccatataac gataaacgct ttaccgcctt ccctttcaaa accgcaaatc
    13681 atcatgacac cttccctttt actatcagga ttgaccttcc gcctcatcct tgccctgatt
    13741 gccgtatccc ttttatgggg cgtttacttt tgggcagtat ccgcatgagc atcattgtcg
    13801 aaaacctgac cgtcagctac cgccgccgac ctgccgtgca ccatgtggac attacttttg
    13861 aagaacatag tatgtgggcg gttttcggtc ccaacggcgc ggggaaatcc acctttctca
    13921 aatccttaat gggattgcag cctatcgata cgggcagcat ccggctggac ggattgaccc
    13981 gtcagaacat tgcctacctt ccccagcagt ccgacatcga ccgctcgcag cccatgaccg
    14041 ttttcgactt ggcggcaatg gggctgtggt atgaaatcgg ctttttcaaa gggataaata
    14101 ccgctcaaaa acaacgcgtt cacgaagcct tggagcgtgt aggaatgcgg cagtttgccc
    14161 gccgccaaat cgcccacctc tcaaacggac aattccagcg tgtccttttt gcccgaatgc
    14221 tggttcaaaa cgccaaattc ctacttctgg acgaaccttt caacgctgtt gatgcacgga
    14281 cgacctacga acttctcgac gtattgcaga aatgccattg cggcggacac gccatcatcg
    14341 ccgtactgca cgattacgaa caagtccgtg cctactttcc caataccctg ctgctcgccc
    14401 gcgaaaaaat tgcggcaggc gcaaccgaga ccattctgac agaaccctac ctcgcccaag
    14461 ccaacgccaa aatgcagcaa caggaaagcc ccgagtggtg cgcctcataa atgccgtctg
    14521 aaaccgaaaa accatgaacc tctacgacct gctccttgcc ccctttacag aattcgactt
    14581 tatgcgctac gccctcgcat ccgtcttctg cctgtccctc agtgccgcac ccgtcggcgt
    14641 attcctcgtc atgcgccgta tgagcctgat aggcgacgca ttgagccacg ccgtcttgcc
    14701 cggtgccgcc gtcggctaca tgtttgccgg cttgagcctg cctgccatgg gtttgggcgg
    14761 cgtagccgca ggcatgctga tggcactgct tgccggactc gtcagccgct tcaccaccct
    14821 gaaagaagat gccaactttg ccgcctttta tctcagcagc ctcgccatag gcgtagtcct
    14881 cgtcagcaaa aacggcagca gcgtcgatct gctgcatctg cttttcggct ctgtacttgc
    14941 cgtcgatatt cctgccctgc agctcatcgc cgccgtatcc accctcacac tgcttaccct
    15001 tgccgtcatc taccgcccgc tcgtactcga aagcatcgac cccctgtttc tcaaatctgt
    15061 cggcggcaaa ggcgggcttt ggcacgtcct ctttctcgtc ctggtcgtca tgaacctcgt
    15121 atccggcttt caagccctcg gcacactcat gtccgtcgga ctcatgatgc tgccagccat
    15181 taccgcccgc ctgtgggcga agcacatggg cgcactcatc ctcctatccg ttctgacagc
    15241 cctgctgtgc ggcttgagcg ggctgctcat ttcctaccac atcgaaatcc cttccggccc
    15301 cgccatcatc ctctgttgca gcgtccttta tctcttttcc gtcatactcg gcaaagaagg
    15361 cggcattctg accaaatggc tcaaaaacca ccgccaccac accacctgac cccgcaagcc
    15421 gcttgaacaa acaggcggct tgttttacca taacagatgt tacgttatat cacaggagaa
    15481 cagttatgaa acacctcaaa ctcaccctta ttgccgcatt gctgaccact gccgcaactg
    15541 ccgcacccct gccggttgta accagtttca gcattttagg cgacgtagcc aaacaaatcg
    15601 gcggagagcg cgtatccata caaaacttgg tcggagccaa ccaagacacg cacgcctatc
    15661 atatgaccag cggcgacatt aaaaaaatcc gcagtgcaaa actcgtcctg ctcaacggct
    15721 taggacttga agccgccgat gtacaacgtg ccgtcaaaca gagcaaagta tcctatgccg
    15781 aagcgaccaa aggcattcaa cccctcaaag ctgaagaaga aggcggacac catcacgacc
    15841 acgatcatga ccacgatcat gaccacgaag gacaccacca cgaccacggc gaattcgacc
    15901 cgcacgtctg gaacgacccc gtccttatgt ccgcctatgc ccaaaacgtc gccgaagccc
    15961 tgataaaggc cgatcccgaa ggcaaagttt attatcaaca acgcttgggc aactaccaaa
    16021 tgcagctcaa aaaactgcac agcgacgcac aagccgcatt taatgccgtc cctgctgcca
    16081 aacgcaaagt cctgaccggg cacgatgcct tttcctatat gggcaaacgt taccatatcg
    16141 aattcatcgc cccgcaaggc gtgagcagcg aagccgagcc ttcggccaaa caagtcgccg
    16201 ccatcatccg ccaaatcaaa cgcgaaggca tcaaagccgt atttaccgaa aacatcaaag
    16261 acacccgtat ggttgaccgc atcgccaaag aaaccggcgt caacgtcagc ggcaaactgt
    16321 attccgacgc actcggcaac gcgcccgcag acacctacat cggaatgtac cgccacaaca
    16381 tcaaagcctt gaccaacgcg atgaagcaat aaaaccgttt gattggtttg aatttgaaaa
    16441 ggcaaaaggc cgtctga
//
LOCUS       DAXHRI010000031        16335 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXHRI010000031 DAXHRI010000000
VERSION     DAXHRI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.031"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(117..2906)
                     /gene="ileS"
                     /locus_tag="ACFM75_000757"
     CDS             complement(117..2906)
                     /gene="ileS"
                     /locus_tag="ACFM75_000757"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC6255340.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYLFKDTAA
                     LAAAFGLASIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSIVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGCKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(3048..3968)
                     /gene="ribF"
                     /locus_tag="ACFM75_000758"
     CDS             complement(3048..3968)
                     /gene="ribF"
                     /locus_tag="ACFM75_000758"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC6255341.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIEADMEAAKCW"
     gene            complement(4267..5562)
                     /gene="tyrS"
                     /locus_tag="ACFM75_000759"
     CDS             complement(4267..5562)
                     /gene="tyrS"
                     /locus_tag="ACFM75_000759"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC6255342.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEAALQAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPVFEISDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(5625..7503)
                     /locus_tag="ACFM75_000760"
                     /pseudo
     CDS             complement(5625..7503)
                     /locus_tag="ACFM75_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(7868..8959)
                     /gene="ychF"
                     /locus_tag="ACFM75_000761"
     CDS             complement(7868..8959)
                     /gene="ychF"
                     /locus_tag="ACFM75_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC6255343.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9073..10749)
                     /locus_tag="ACFM75_000762"
     CDS             complement(9073..10749)
                     /locus_tag="ACFM75_000762"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC6255344.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVAINRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RVIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPVCMAKTQYSLSDNAKLLGCPEDF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            10924..11355
                     /locus_tag="ACFM75_000763"
     CDS             10924..11355
                     /locus_tag="ACFM75_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6255345.1"
                     /translation="MSSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(11586..12281)
                     /gene="murU"
                     /locus_tag="ACFM75_000764"
     CDS             complement(11586..12281)
                     /gene="murU"
                     /locus_tag="ACFM75_000764"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC6255346.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12297..12797)
                     /gene="hpaC"
                     /locus_tag="ACFM75_000765"
     CDS             complement(12297..12797)
                     /gene="hpaC"
                     /locus_tag="ACFM75_000765"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC6255347.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(12912..13352)
                     /gene="nadR"
                     /locus_tag="ACFM75_000766"
     CDS             complement(12912..13352)
                     /gene="nadR"
                     /locus_tag="ACFM75_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC6255348.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(13695..14204)
                     /locus_tag="ACFM75_000767"
     CDS             complement(13695..14204)
                     /locus_tag="ACFM75_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC6255349.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTANDEDGDLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14283..14358)
                     /locus_tag="ACFM75_000768"
     tRNA            complement(14283..14358)
                     /locus_tag="ACFM75_000768"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14323..14325),aa:Lys,seq:ttt)
     gene            14574..15653
                     /gene="apbC"
                     /locus_tag="ACFM75_000769"
     CDS             14574..15653
                     /gene="apbC"
                     /locus_tag="ACFM75_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC6255350.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSNGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT         3681 a         4455 c         4446 g         3753 t
ORIGIN      
        1 ttccgacaac accgcaatct tgaaacccct ccgccgttat aaagacaaat cgcggcacaa
       61 aaaatgccgt ctgaaatgct gttcggcggt ttcagacggc atttgctcaa actttatcag
      121 gcgtaatggc gcgtttcgcc ttctccgccg acattctctg cacagcgttt gcagacggtt
      181 tcatagcctg caaccgcgcc cacatcgcgg gtgtagtgcc agcagcgttc gcatttttca
      241 ccatcactgg ctttagcggc aacggcaagt tcgctgccta ctttcacttc tgctttagac
      301 accagcaaag caaagcgcaa ttcttcgccc aaagcattca gatagccggc catttcttcc
      361 ggcgcggtaa tttcggcttc ggcttgcaag gacgaaccga cggttttgtc ggcgcgcaaa
      421 ggctcgatgg cggcggttac cgcttcgcgg gcttcgcgga ttgccgtcca ttttttcacc
      481 agttcggctt cggttttttc gttgatggtc gggaactcgt gccaagtatg gaagaggacg
      541 ctgtcttctt cgccgccgcc gatgatgtcc cacgcttctt cgccggtgaa gcacaaaatc
      601 ggtgcaatca agagaaccaa actgcgtgtg atgtgataca gggcagtttg tgcgctgcgg
      661 cgtgcatggc tgtcggcttt ggtggtgtag aggcggtctt tcaggatgtc gaggtagaac
      721 gcacccaagt cttccgagca gaaagaaaca atgtctttta cggcaaagtg gaaggcataa
      781 cgcggatagt aatcgcctgc cagacgctct tgcagctgac gtgccaatac cacggcgtag
      841 cggtcgattt ccaccatatc cgcctgttgc acggcatctt caatcggatt aaagtcgctc
      901 aagttggcaa acaaaaagct caaggtattg cggatacggc ggtaactttc ggttacgcgt
      961 ttgaggattt ctttggaaat cgccaattcg ccgctgtaat cggtggatgc tgcccacagg
     1021 cgcaggatgt ctgcgccgaa ttcgttatag acttcttgcg gcgcgacgac gttgccgatg
     1081 gatttcgaca ttttgcagcc gttttggtca accacgaaac cgtgggtcag cagctgtttg
     1141 tatggggcgc ggcccatgga tgaggcacaa ccggtcaaca tagaggattg gaaccagccg
     1201 cggtgttggt cgctgccttc gaggtacaag tcagcaggcc attccaattc ttcgcgttgt
     1261 ttcacaatgg aataatgggt cgagcctgag tcgaaccaca cgtccatcgt atcggggagt
     1321 ttgtcgtaat gttcgcaatc ttccgcgctt aataattcgc ttttatcgag ggagaaccaa
     1381 gcctcgatgc ctttttcttc aatgcgttgc gcgacttttt ccaaaagttc ggcagagttc
     1441 ggatgcagct cgcccgtttc tttgtgaaca aagaaagtca tcggcgtgcc ccaatagcgt
     1501 tggcgtgaaa ccacccagtc aggacgacct tcaatcatgg cttccaaacg cgcgcgaccc
     1561 caagacggga agaattcggt gtcgtccacg gccttgatgg ctttgtcgcg cagggttttg
     1621 ccgtcggcac cggctttgtc cataccgaca aaccattgac ctgtcgcgcg gtaaatcagc
     1681 ggcgttttgt gccgccagca gtgggcgtag ctgtgttcga ttttactgct tgccaaaagg
     1741 ttgccggttt cttccaacca ttgcaggatg acggggttcg cctcccaaac gcgcataccg
     1801 gcaacgcgcg gcgtttcgcc gatgtatttg ccttcggcgt tgacaggatt gtaaagctcg
     1861 atgccgtatt tgttgcagac ggcgtagtct tccaaaccgt gcgcgggcgc agtatgcacc
     1921 aagccggtac cggcatcggt ggtaacgtgt tcgccgttaa gcatgggaat atcgcgttcg
     1981 aggaacggat ggttcatgtg caggttttcc agcttgtcgc cggtggtttc ggcaaggatg
     2041 gcgatgccgt ctgaaaagcc gtaacgtttg agcgcgtctt ccgccaaatc tttcgccaat
     2101 accaatttgc ctttgggcgt atcaatcagt tgatacacca cgtccgcgcc ggcagatacg
     2161 gcttggctcg caggcagcgt ccaaggcgta gtcgtccaga tgacggcaaa cgctttgcct
     2221 tcgatacttg ccaaaccgaa tgcggcggca agcgcggcag tgtctttaaa cagataggca
     2281 acgtcaatcg cgggcgatac tttgtctttg tattccactt ccgcttcggc cagcgaagaa
     2341 ccgcagtcca agcagaattg aaccggtttc gcaccccggt agagatagcc ggatttgtag
     2401 atttcgccga gcatacgcac ggtatcggct tcggttttga aatccatagt caggtaagga
     2461 tggtcccagt cgcccaacac gcccaagcgg ataaagtctt ttttctgacg ggcaatctgt
     2521 tcggcggcgt attcgcggca caattcgcgg aaacgtgctt taggcatgtc tttgccgtgc
     2581 agtttttcta ccatcacttc gataggcaaa ccgtggcagt cccaacccgg tacataaggt
     2641 gcgtcaaaac cggcttgggt tttgctgcgg ataatgatgt ctttgagaat tttattgacg
     2701 gcatgaccga tgtggatgtc gccgttggca tacggagggc cgtcgtgcag aataaatttc
     2761 ggacggcctt tggcgatttc gcgcagtttt tggtagcgtt tttgctcgta ccagcttttc
     2821 agccatgcag gctcgcgctt ggcaagattg ccgcgcatcg gaaacgggct ctcgagcagg
     2881 ttgacggttt tgctgtaatc ggtcattttt taatctctat tgttacaata tttcggtttc
     2941 agacggcatt gcgcctcaaa cagtatttca taacgggaaa cccctatgcc gtctgaaaag
     3001 ttaaaagatt gattgtagcc caatcggatg gtttgtataa ggtttttcta ccaacacttt
     3061 gcggcttcca tatcggcttc aatctgcctt ttcagttctg ccataccgtc aaacttttct
     3121 tcatcgcgca gtttgtgcag gaagcggacg ttcagccgtt gtccgtacag gtcgccttga
     3181 aagtcgaaca ggtggacttc aagcttttga gaacagccgc tatcaacggt gggattgaag
     3241 ccgaagctcg ccacgccgcg ccgcgtgccg aacgcgccgt ccgcttcgac gacgaacacg
     3301 ccgccgagtg cataacggtg gcggggcagg cggatgttgg cagtcggggc gtttaaggtg
     3361 cgtccgagtt ttctgccgtg caccaccctg ccgctcaaga cgtagtcgtg tcccaaaagt
     3421 tttttcgcat aggcaaggtt gccgtctgaa agggcttgtc gcacggcggt actgctggtg
     3481 cggatgtctt cgacgatgac ggaaggcgta cgctcggtct gcatatcggg ctgttgtgcc
     3541 aaaagttcaa aacagccttc ccgccccgca ccgaaacgga aatcgtcgcc gacgagcaaa
     3601 taacgcgtat tcaaggtttg acgcagcagg cggtcgataa acccttgcgc ggatatttcg
     3661 gaaaaatttt gatcgaaacg caaaacccag acggcatcga cacagcctgt gccttccaat
     3721 aattcgagct tggtgcgcag ggggctgatc cgacacggtg gcatcctgcc ggtgcggagt
     3781 gcgaaaaatt ctttgggttg gggttcgaaa acgacggtca cgacgggtaa tccgcgcgta
     3841 tcggcttcga ggcggagttt ttggaggatg tgtttgtgtc cgaggtgtac gccgtcaaaa
     3901 ttgcctatgg ttacggcggc cccgtgcgga aagtcgggcg cgttgtgccg ccccagcctg
     3961 attctcattg ttatgttcgg gtatgtggtg aaacaggcgg tcattgtaaa cggtattgcg
     4021 gtttatagac agtgtgccgc ccgttacgcc cgcccgacgc gggaaaagta ggcaaatttc
     4081 ccgcagccga acgcgccaaa cgcataaagc gcgcagcagg cgcggtgcta tgtgttgaaa
     4141 catcgcccca aacctgatgc aacatcagtg aaaatcgttc ttttttaacc aggtaaaccg
     4201 agacaagcaa ccctccgccg tcattcccgc ctgcgcggga atgacggcat ttctgcggca
     4261 atcggattat ttccaaacca aaagcgcgtg gttgcgtttg ccgcgccgaa ggatggtgta
     4321 tttgccgaaa cgtttgtgtt cgtcggtcag caggcaggca tcgtcggggc gttcggcggc
     4381 gtggttgggg ttgttggctt cggcaggttt gccgttgagc agaaccgctt tggcgttgac
     4441 aaagccgcgc gcttctttgt tggacgctgc caaaccggtt tttaccaagg cttcgacggc
     4501 gttgatgccg tctgaaattt caaatacggg caggccgtcg agggcgagct gctcgaagtc
     4561 gctttcggtc aggctgcttt ggtcttcggc aaacaggctt tcggaaatgc gctgcgcggc
     4621 ttgcagggcg gcttcgccgt gaatcaggcg ggtcatttct tcggcgagga tgcgttgcgc
     4681 ttcgggcttg ctgccgcttg cctggtcttt ggcttcgacg gcatcgattt cttcgatgga
     4741 caggaaggta aagtatttca ggaatttata cacatcggca tcggcgactt tcagccagaa
     4801 ttggtagaac tgatagggcg aggttttttt cgcgttcagc catactgcgc cgccttcggt
     4861 tttgccgaat ttggtgccgt ctgatttggt aaccaaaggc agggtcaggc cgaacacttg
     4921 tttttgattc aggcggcggg tcaggtcgat accggcggtg atgttgcccc attggtcgga
     4981 gccgccgatt tccaaaaccg cgccgtggcg tttgttcaac tcggcgaagt cgtagccttg
     5041 cagcagggag taggcgaact cggtgaagga aatgcctgcg ccgtcgcggt caatgcgctg
     5101 tttgacggat tctttgttca gcatggcgtt gacggagaaa tgcttgccga tgtcgcgcag
     5161 gaagtcgagg cagttcattt tgccgaacca gtcggcgttg ttcgccataa tggcggcgtt
     5221 tccgccttca aagctcaaga aaggggttaa ttggctgcgg atgctttcca cccagccggc
     5281 aacggtttcg gcggaattca agctgcgttc ggcggctttg aagctggggt cgccgatcat
     5341 accggtcgca ccgcccacca gtgcaatcgg cgtatgcccc gcctgttgga agcggcgcaa
     5401 tgccaatacg ggcagcaggt gtccgatgtg caggctgtcg gcggtcgggt cgaagccgca
     5461 ataaagggaa attttttgtt cgttcaacaa agcgtctaag gcttcgatgt cggtggtttg
     5521 cgcgataagg ccgcgcgatt gcaggtcttg gatgacgctc atcggtctct ttcaaaaaaa
     5581 attagcggtt ttgcaaaccg ccgattgtaa caaatttaag cgaatcaatg gttatggcgc
     5641 gtatcgagaa accgttgttt ttcggaaaaa cgcttcgcca attccgtgcc gccgtaaggg
     5701 ttgatgtggt ctttgtccga gtaaaccggc aatccgccga tttggaaatt tgcggggata
     5761 taggcggcgg catcaataat atagacgttg gggtatttgg ctgccaattc cctgatgcgt
     5821 gcattggctt tcagagtgct ttcgtcgtcc gggcgcaggg tttggcggta acccggtatg
     5881 cgtgaagaca agatataggc gcgctggacg ttgtaagacg aggcaaggtt gtccgccatc
     5941 aggtaaacgg cttgtttttc ggacgagagt ttatgcagca tacggtcgaa ttttttgaaa
     6001 aaaccggcat cgtaggcaag ggagcggctg ttttcgggca tttggctgcc ccaccgcatc
     6061 gccaaaacca cttttgaata ccggggcagg tgttcttcgg catagcggta aacggcgcgg
     6121 caggcggccc agttttggaa cacgcgggac gcgtagcctt ccacataggc gcaagcgtcg
     6181 gcggaaacca tagtggcgga ccatttttct tttttgccca cggcatcgaa gaatgttttg
     6241 taatggtcgg cgtgggagtc gcccaaaacc agcagctccg gctgtttttc cgtatccccc
     6301 cacaggcatt gtttgccggt attgttgtgg caggaggtgt tggaacgcgt cagccccaag
     6361 cggtcgtatt gcgccataaa cggcagtctc atcgcaaaaa acgagcccgc ccccaaaatg
     6421 agcataggca aggcataaat ccataaaacg gattgtgcga acgaaccttg ccatttttta
     6481 aacggttttt cgatgcagtg gtaagaaaac agggaaagca gcaatatcag gacgaccgcc
     6541 gccgccggcg aataaggcgg caggttgtcc gggccgatat agcgcataaa ggccaatatc
     6601 ggccaatgcc acagataaag cgaataggaa atcaaaccgg cggcaacggt gattttccat
     6661 tggaaaaatt ttttaagcgg gtgttcgtaa tggttgaaat aaatcagcgc ggcaacagcc
     6721 agacagggaa tcaaagcggc ggggcccggg aaataggcgg tttgttccga ataggcaaac
     6781 aggcaggcgg acagtatgca cgcggaaaac aatgcgccgg cggcggcaca gcatctgccg
     6841 acggcgagat tccgctgttg ccggtaccgc atccacacgg cggtcagcga tccgaccagc
     6901 atttcgcagg cgcgcaggtg gggcaggtaa tatttatcga acgcggaagg cataaaggaa
     6961 gcggcaaggc ttaaggcgca cagtgcggcg aggaagccgg cttggacgcg cgggcttttg
     7021 cgggcgacaa gcagcagcaa tatcggaaag acaaagtaaa attgttcttc gaccgacaaa
     7081 gaccaaatgt gcagcagggg cttttcttcc tgcgcgggag cgaaatagtc cttgcccctt
     7141 gcaaaataca ggttggatgc gaaacccaag gcggtcagcg cggatttcca caaaagaaag
     7201 aaatcatctt tggtgaataa aaagaagccg cctatcagcg ttgccgccaa tacggcgaaa
     7261 aatgcgggca gaatccgctt gatgcggcgg atataaaatg ccttcaggga aaacctcccc
     7321 cccccccgac atttcgcgaa ggaggatcgc cgtcatcaaa aagcctgaaa tcacaaagaa
     7381 tatatcgaca ccgagaaatc cgcccggcag ccaatttttt tcgatatgga acacgatgac
     7441 ggacaaaacg gcggcgacgc gcaatgtgtc gatgtccggg cggtagggta aggcttggct
     7501 cataatgttt ttatagtgga ttaacaaaaa ccagtacggc gttgcctcgc cttgccgtac
     7561 tatctgtact gtctgcggtt tcgtcgcctt gtcctgattt aaagttaatc cactatactc
     7621 gaaacgcggc ggcgcaaatg ccgtctgaaa ggtcattttg tacgggatat tcggaatgcc
     7681 ggacggcttt ttgcaacagc ggggcaggcg gtttgttttg caggaatcgg ggagggcaaa
     7741 tcggaaatgc gggtgggagt ttattttgat gcggctgcat tccggcggta cgggaaacgc
     7801 cgaaaatcat caaaatcggc ttcagacggc atttccggca agccgcctga aacctgccgc
     7861 atttgggtta cacgttaaac aaaaagtgca tcacatcgcc gtcttgcacg acatattcct
     7921 tgccttccac acgcattttg ccggcttctt tggctttggc ttcgccgccg agcgagacaa
     7981 agtcgtcgta agaaatgact tgggcgcgga tgaagccgcg ttcaaaatcc gtatgaatca
     8041 cgccggcggc ttgcggcgcg gtgtcgcctt tgtgtatcgt ccacgcgcgg acttctttca
     8101 cgccggcggt gaagtaggtt tgcagcccca aaaggtcgta gccggcacgg atgaggcggt
     8161 tcaggcccgg ttcttccaaa cccatttcgg cgaggaattc ggctttttcg tcgtcttcca
     8221 attcggcgat ttcgctttcc atcgcggcgc atacggcgac gacgggggcg ttttcttttg
     8281 ccgccaattc tttcaggcgg tcgaggtgcg gattgttttc aaaaccgtct tcggcgacgt
     8341 tgcccacata catcgcaggt ttggctgtca gcaggaacag cggtttgagc atcgcgcgtt
     8401 cttccgcgtc caaaccgaag gaacgcacgg gtttgccttc gtccagatgc ggcagcagtt
     8461 ttttgcacaa atcgaccagc ttttgcgcgt ctttgtcgcc tgaacgggcg cgtttttctt
     8521 cgcggacgat ggctttttcc acacttgcca ggtcggcaag tgccaactct gtgccgatgg
     8581 tttcgatgtc ggcaatcgga tcgacgcggc ctgcaacgtg gacgatgttg tcgtcgtcaa
     8641 agcagcgcac gacattcaca atcgcatcgg tttcgcggat gttggcaagg aactggttgc
     8701 ccaagccctc gcctttgctc gcgcctgcaa ccaaaccggc aatatcgaca aattcgacga
     8761 tggcaggctg cattttttgc ggattgacga tttttgccaa ttcggccata cgcggatcgg
     8821 ggacttcgac gatgccgacg ttgggttcga tggtacagaa aggatagttt gccgcttcga
     8881 tacccgattg ggtcagcgcg ttaaaaaggg tggatttgcc gacgttgggc aaaccgacga
     8941 tgccgcattt caaactcatg ttttttcctg aaaatagaga aatttaacgg cggattatag
     9001 cataccgccg cccgcgttcc gaaaaaatgc cgtctgaaac ggcttcagac ggcatctggt
     9061 ttcaaaaacc gttcagaaca agccgtgaat cacgccgtgt tcgtccacat cgattttctc
     9121 ggcggccgga actttcggca gacccggcat cttcatcata ttgccgcaca gcgcgacgat
     9181 gaagcccgcg ccggcggaaa cggtgatgcc gcgcacggcg atgcggaagt cttcggggca
     9241 gcccaacagt ttggcgttgt cgctcaaaga gtattgggtt ttcgccatac agaccggcat
     9301 tttgtccaaa cccagttttt ccagcgaggc gatttcggca gacgcttccg cgctgaaatc
     9361 aacatcttcc gcgccgtaca ctttttgggc aatcacacgg attttgtctt tgatgcccaa
     9421 ctcgacatcg taggcgaaac cgaagttatt ggtttgactt tcaatggcgt tgacgacttt
     9481 gcgcgccaaa tccgcgccgc ccgcgccgcc tttgccccac acttcggtca gggaaacttc
     9541 aacgccgtgt tcggcacagg ctttttcaat catcgccaac tcggcatcgg agtcggacac
     9601 gaagcggttg atggcgacga cgacgggcag accgaatacg tttttcaggt tggaaatgtg
     9661 tttcagcagg ttgggcaaac ctttttccaa agcgtctaaa ttttcttcgc cgaggttggc
     9721 gcgttccacg ccgccgttat atttcaacgc gcggacagtc gccacgacaa cagccgcatc
     9781 aggtttcaaa ccggcaaggc ggcatttgat gtcgcagaat ttttccgcgc ccaagtccgc
     9841 gccgaagcct gcttcggtta cggcgtaatc ggcaaggtgt ttcgccagac gggttgcggt
     9901 tacggagttg cagccgtggg cgatgttggc gaacgggccg ccgtgtacga aggcgggcgt
     9961 gccttcgatg gtttgcacca agttgggctt aatcgcatct ttaagcaatg ccgccatcgc
    10021 gccgtgtgcc ttcaaatctt tggcataaac ggggctgccg tctttggcgt aggcgacaag
    10081 gatgttgccc aaacgctctt tcaaatcgct gatgtctttg gcaagacaga ataccgccat
    10141 cacttcggag gcaacggtaa tatcgaaacc gtcgggacgc atcacgccgt caacaggttt
    10201 gcccatgccg tcgatgatgt tgcgcaactg acggtcgttc atatcgacca cgcgccgcca
    10261 cagcacgcgt ttggggtcga tgtccaactc gttgccctga tagatatggt tgtcgagcat
    10321 cgcggcaagc agattatttg ccgcaccgat ggcgtgaaaa tctccggtga agtgcaggtt
    10381 gatgtcttcc atcggcaaaa cttgggcata gccgccgcct gccgcaccgc ctttaacacc
    10441 gaacaccggc cccagagaag gttcgcgcag ggcaatcacg gcatctttgc cgatgtggcg
    10501 caacgcgtcc gccaaaccga tggttacggt ggttttgcct tcgcccgccg gagtcggatt
    10561 aatggcggta accaaaatca gcctgccctg tttttgcggc agtttgaacg cttcggcagg
    10621 attgattttc gccttgtaat gaccgtaagg ctcaatgttg tcggcattca gacccagctt
    10681 ggcggcaatt tcgccaatcg ggcgcatggt ggaggattgg gcgatttcgg catcggtttt
    10741 gaagctcatg attttccttt agaaatgagg agggacatgc cgtctgaaag catcaggcga
    10801 caaacaggtg gattgaaaat aatatcaggc atattataac gttatccgca ccaaacccgc
    10861 agtgaaattt ttgacgctgc aacaaaaata ccgttcatat tgttcacaat ccaaggagaa
    10921 aacatgagca gcaacgcatg gctgttttgg gcattggcat cggcaggctt cgcctcattg
    10981 accgccgttt tcgccaaaat gggtttacag ggcatcgatt ccgatttcgc cacctttatc
    11041 cgcaccttgg tgatcctcgc cgctttggta ttgtttttga cctacaccgg caaatggcag
    11101 ggtgtgaacg gctttacggg gcgcaactgg acattcctta tcctatccgg ccttgctacc
    11161 ggcgcatcct ggctcgccta cttcaaagcc ctgcaactgg gcaacgcctc gcaagtcgcc
    11221 cccatcgaca aattcagcct ggtcttggtc gcgctgatgg cggtggtttt cttggacgaa
    11281 cgcccgaaca cgcaggaatg gataggcttg gggctggtaa cggcgggcgt gttggtgctg
    11341 gcgttgaaac gttaaaccga atccgccata ccgcctgaaa ccgggttttg acaatctgac
    11401 gggcggcggt ctgaacaaag ttacccaata aaatccgaca ccgcgccgac ggcaaaccgc
    11461 cgcgcttcag atggcatttg ttggacggaa agccccgaac gccctatttt cacgcctcca
    11521 cgaaaatatg ctggacatcc ggtcggaacc gttcggcagc cggctcggca agccaccgtt
    11581 ttatttcact tccaagcccc tgcaagggct tgagcctctt tcagacggca taccgtgccg
    11641 acatccagcc acaagcccgt atgcttctga ccgctcacgc ggttttgccg catttcgcca
    11701 cgcaatacgg gcgcgagttt cgccacactg cccgcttcga ttccgtcaaa catttcagga
    11761 cggtaaatac ccacgccgct gaatgtcaat ccgttgccgc catttacttc cggccgcacg
    11821 ctgccgtcgg gcagcaggga aaaatcgccg tcggggttgt gcggcggatt ttccaccagc
    11881 cacagatggg cggaaatatg ttccggcagg gacgatgccg cctgaaacgc ggcggtaaaa
    11941 tcgatgtcgg tcagcacgtc gccgttgacc accaaaaacg gctgcccacc caacagcggc
    12001 aatgcctgcg cgatgccgcc tgccgtttcc aaaccgcctg cgggttcggg cgaataggcg
    12061 atgttcacgc cataagccga gccgtcgccc aaagcatctt ctatctgccg gcccagccaa
    12121 gcgtggttga tgacgatttc ggtaaacccc gcctgcttca gacggcatag gtgccaaccg
    12181 attagaggct tacccgccac atcgagcagc ggcttcggag tggtatcggt caaagggcgc
    12241 atacgctcgc cgcgtcctgc cgccagtatc atcgctttca tatatctgtc cgaatatcag
    12301 tctaaaaatc taaactgccg tctgaaatac agcagcgcgg ggcgtttgca cccgcagttt
    12361 ttgatttcgt cgagcctgac gtaaaacaca aaatgcgtgc cgatttcatg tttgccgaca
    12421 atatgcccgt gcaggtgcgc caacgcgccc tctatttcaa gttgtcccgt tttgccgcga
    12481 tgccagatgt ggtaggcaaa ccgctcttcg ggcgacagcc cggtcagccc ggcaaaatgt
    12541 tcggcaacat cctgatgttc gtctgccaac gtattgatgc acaggctgcc gttttccgac
    12601 agtatcggga tgatacgggc tttacggttg atgcacagca tcacggtcgg cggctcgtcc
    12661 gtaaccggtg taaccgccgt cattgtaatg ccgtaacgcc ctgccgcacc gtctgtcgtg
    12721 atgacatgaa cgcctgccgc acaggatgcc atcgcatcac ggaacgaagt ttgaaaagtt
    12781 ttctgcaaat ccgccatttt tcccctttaa actgtcccct atataagaat gctgcacaca
    12841 aggcaccccc catgtgcagc agttctgatt caaaaagccg tcggtcggac gtttccgcgc
    12901 gttacggcgt attacgagtt caacgcatcc tcgattttgg caagttctgc caacaggtct
    12961 ttaagcagca gcattttctc gcggcccagc acttcctcga tagcgtcgta acgctcgtcc
    13021 acttcttcgc cgatttcctc atacagcttc tcgccctcgg cagtcagctt cagaaaaaca
    13081 cgtcgttggt cgttggaagg tttcaggcgg acaaccaaac ccgctttttc aaggcgggtc
    13141 aggataccgg tcaggctggg gcgcagaata cacgcctgat tcgccaaatc ttgaaagtcc
    13201 agcgtgccgt tttccgccaa aagacggata atccgccatt gctgatcggt aatattcgcc
    13261 tgattcagaa taggcctgaa ttgggtcatc agggcttccc ttgcctgtat cagactgata
    13321 ttgatagacg catgttttga ttgggtaggc attgttcaag tctccaagtt atcgaaaatc
    13381 aaactttcaa accgtcggga aagcctgtgg gcgtaaattt tgatgcaacc gttatataac
    13441 aaaacgaaca tatagcaaca atacgctata aaccgcatcg gacgactggg tataaaagac
    13501 tttaattccg ataatcctat ctaaaaatat tttaatagtt atatcttagt ctattttccc
    13561 tacaatctca acaaagtatt acatttactg tcgaaagaac tttcttccag aaaaaacaaa
    13621 agccgccgca tcagacgagc aaggtatatg ccgtttgatt ctctacatag cgtaaagttt
    13681 aacaaaaaca aaagttaacc gaaaacatcc gcctgaaaaa ttcgtgcgcg caagccccaa
    13741 taactgctga ttcccgtcgt ataatggacc attttcccat ttttgaccaa aacgacggca
    13801 ggcgttgcga caatccgcca agaccttgcc aaatccccgt cctcatcgtt ggcagtcgga
    13861 aagcccaagc cgcgttttgc catatacgcc gccacttccg ccgaactgcc ggaacgtacc
    13921 gccacgccga cgaccggcac gccgtccgcc gccaaatcat cgattatcgg cgactgataa
    13981 cggcacacgc cgcaccagct cccccaaaaa tacaccaaaa ccgccttatc tcggctaaac
    14041 tgtcccaaag tcagccgctg ccccgacagc agggtcaaag gccgccctgc cgcaccggca
    14101 ggttcgtcgg gcttgcgtat ccaatccaaa aacagcgaca ccaataaaaa caccaatgcc
    14161 gtctgaacgg caaatttgat gcccgaaagc agtttctttt tcatacgctc tctcaaacgg
    14221 tacgcccacg caaccggcag gcaaacaaaa agccaagtct caaaacttgg cttccggtta
    14281 tctggtgggt cgtgagcgat tcgaacgctc gaccaacgga ttaaaagtcc gctgctctac
    14341 cggctgagct aacgacccga taagccgtgc attatacagc accatcctgc ctcgtcaagc
    14401 aaattttaca ggcttaattg cagaccactg tttgcacggg atattttgac aacggatttt
    14461 cacaatccgc cgcataccgt gtaaaagttc gcacaaggaa aggcaaaccg cccgaaatca
    14521 atgtacactt tccgcccgtt tcccttccca acctgcacac agaaacacac attatgaaca
    14581 tacaaaacat ccgcaccctc ctcgacaccg tcgccgttcc gaatacggca cgcacgctcg
    14641 gcggcgaaaa ggccgtccgt tcggtcgaac agcgttcaga cggcatccat atcgccctgc
    14701 atttcggctt ccccgtcgcg cacattgcct cagaaacagc cgaccgcata caggaaatcc
    14761 tgatgccgct gacaggcgac acacacatcc atctgtccat ggacaccgaa atcggcacac
    14821 acaaagtcca gcccggcgtt accactatca aaggtgtgaa aaacatcatc gccgtcgcat
    14881 cgggaaaagg cggcgtgggc aaatcaacca ccaccgccaa ccttgccgcc gcaatggcgc
    14941 gcatgggcgc gcgcgtcggc gtactcgatg ccgaccttta cggcccgagc caaccgacca
    15001 tgttgggtgt ggacgaccgc aaacccgacc agaaaaacca aaaactgatt cctgtcgaat
    15061 cttcaaacgg catacaggtc atgtctatcg gctttctcgt cgataccgac caagccgtcg
    15121 tctggcgcgg gccgatggtc agccaagcct tgcagcaact gatgtttcaa agcgagtggg
    15181 acgaagtgga ttatcttttc atcgacctgc cccccggcac gggcgacatc cagctcacgc
    15241 tgtcccaacg catccccgta accggatcgg tcatcgtaac cacgccgcag gacatcgccc
    15301 tgatagacgc gcgcaaagcc gtggatatgt tccgcaaagt caacattccc attttgggcg
    15361 tattggaaaa tatgtccgtc cacatctgct ccaactgcgg gcatagcgaa gcactgttcg
    15421 gcacggacgg cggcaaagat ttagccgcac gcctcaacgt ccccctgctg ggacagcttc
    15481 cattaagcct gcccgtgcgc gaagccatgg acggcggcac accggcgcaa ctgttcgacg
    15541 aacaccccgc catcgcccga atctacaccg atgccgcatt ccaaatcgcc ctgggcattg
    15601 ccgacaaagg caaagacttc agcagccggt tccctaaaat cgtcgtcgaa taaagccgcg
    15661 tccgaaaccg caacagcaat gccgtcccaa gccccgcgcc tgccggcggg cttcatagtc
    15721 ctctctcccc gtgggagagg gctggagaga gggcaacaag ccgcaaggct tgtattgggg
    15781 cggttaagct gttgggaaag attgccgcaa cttggagaat gccctctccc cggccctccc
    15841 ccacagggga gggagcggat tgcggcaggt tttgcggttg caggcggttt gaaaaacaga
    15901 ttggatttac aaccgttgat gcaggtcgtc tgaaaagcaa aaagcagcct gcacaacctg
    15961 attagtttac aaaaccctta accccaacag cctccccgtc ctctctcccc gtgggagagg
    16021 gctggagaga gggcaacaag ccgaaaggct tgtattgggg cggttgagtt gttgggaaag
    16081 gttgccgcaa cttggaaaat gccgcctccc cggccctccc ccacggggga gggagtgggt
    16141 ttcggcagat ttttcggttg caggcgattt gaaaggcaac ttagatttgc agctgttgtt
    16201 tcaggtcgtc tgaaaaataa aaagcagcct gcacaacctg ttttccttgc agcaaacctc
    16261 aaccccaaca gccgacccgt cctctctccc tgtgggagag agctagagag agggcaacaa
    16321 gccgaaaggc ttgta
//
LOCUS       DAXHRI010000032        16253 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXHRI010000032 DAXHRI010000000
VERSION     DAXHRI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.032"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..108
                     /locus_tag="ACFM75_000770"
     CDS             <1..108
                     /locus_tag="ACFM75_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6255351.1"
                     /translation="TVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            115..190
                     /locus_tag="ACFM75_000771"
     tRNA            115..190
                     /locus_tag="ACFM75_000771"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Trp,seq:cca)
     gene            305..583
                     /gene="secE"
                     /locus_tag="ACFM75_000772"
     CDS             305..583
                     /gene="secE"
                     /locus_tag="ACFM75_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC6255352.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            585..1121
                     /gene="nusG"
                     /locus_tag="ACFM75_000773"
     CDS             585..1121
                     /gene="nusG"
                     /locus_tag="ACFM75_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC6255353.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQK
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1222..1656
                     /gene="rplK"
                     /locus_tag="ACFM75_000774"
     CDS             1222..1656
                     /gene="rplK"
                     /locus_tag="ACFM75_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC6255354.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1656..2351
                     /gene="rplA"
                     /locus_tag="ACFM75_000775"
     CDS             1656..2351
                     /gene="rplA"
                     /locus_tag="ACFM75_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC6255355.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2581..3081
                     /gene="rplJ"
                     /locus_tag="ACFM75_000776"
     CDS             2581..3081
                     /gene="rplJ"
                     /locus_tag="ACFM75_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC6255356.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3139..3510
                     /gene="rplL"
                     /locus_tag="ACFM75_000777"
     CDS             3139..3510
                     /gene="rplL"
                     /locus_tag="ACFM75_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC6255357.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3701..7879
                     /gene="rpoB"
                     /locus_tag="ACFM75_000778"
     CDS             3701..7879
                     /gene="rpoB"
                     /locus_tag="ACFM75_000778"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC6255358.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDKDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            8036..12211
                     /gene="rpoC"
                     /locus_tag="ACFM75_000779"
     CDS             8036..12211
                     /gene="rpoC"
                     /locus_tag="ACFM75_000779"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC6255359.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12503..12760
                     /locus_tag="ACFM75_000780"
     CDS             12503..12760
                     /locus_tag="ACFM75_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255360.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            12757..12906
                     /locus_tag="ACFM75_000781"
     CDS             12757..12906
                     /locus_tag="ACFM75_000781"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6255361.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            13085..13456
                     /gene="rpsL"
                     /locus_tag="ACFM75_000782"
     CDS             13085..13456
                     /gene="rpsL"
                     /locus_tag="ACFM75_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC6255362.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13574..14044
                     /gene="rpsG"
                     /locus_tag="ACFM75_000783"
     CDS             13574..14044
                     /gene="rpsG"
                     /locus_tag="ACFM75_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC6255363.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14063..16168
                     /gene="fusA"
                     /locus_tag="ACFM75_000784"
     CDS             14063..16168
                     /gene="fusA"
                     /locus_tag="ACFM75_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC6255364.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4322 a         3609 c         4208 g         4114 t
ORIGIN      
        1 accgtagaac tgattgcgcc tatcgctatg gaagaaggtt tgcgctttgc gattcgcgaa
       61 ggcggccgta ccgtgggtgc cggcgtggtt tcttctgtta tcgcttaagt ttagaggcca
      121 atagctcaat tggtagagta tcggtctcca aaaccgaggg ttgggggttc gagaccctct
      181 tggcctgcca aataaaaaat taaccggcct tgtgtcggtt aatttttttg tatttgttat
      241 ttagtaaact ctcttgccat ttacatggat tgagaataga cagatgctat gatggataaa
      301 taatatgaca gaacatacgc ctgaaaaaaa gaacgttaaa gtggatcaac tggttgttca
      361 agataaagaa tctgcatcta attccggtaa ggaagggttt tttgcatatt tctcaaattc
      421 ttggtccgaa ttcaaaaagg tggtttggcc taagcgtgaa gatgctgtca gaatgactgt
      481 atttgttata gtgtttgttg ctgtgctttc tatatttatc tatgcggcag atacagcaat
      541 ttcgtggtta ttttttgatg tattgctgag aagggaaggt tgagatgtcg aaaaaatggt
      601 atgttgtaca ggcgtattcg gggtttgaga agaatgtcca acgaatattg gaagagcgca
      661 ttgcccgtga ggagatggga gattatttcg gacaaattct ggtgcctgta gagaaagttg
      721 ttgatatccg caatggtcgt aagactatta gtgaaagaaa gtcatatcct ggttatgtgc
      781 tagttgagat ggaaatgaca gatgactctt ggcatcttgt aaaaagcacc ccccgtgttt
      841 ccggttttat tggagggagg gctaatagac ctacgccgat tagtcagaaa gaggctgaaa
      901 ttattttaca gcaggttcag accggcatag agaagccgaa accaaaagtt gaatttgagg
      961 tcggtcaaca ggttcgtgta aatgaaggcc cgtttgcgga ttttaacggg gtggttgagg
     1021 aggtcaatta tgaacggaat aagttacgcg tgtctgttca gatatttggt agagaaacac
     1081 ccgttgagct ggagttcagc caggttgaaa agattaactg atttttatac ttgaaaaaaa
     1141 agcaataaga ggatagaatc aaaaattaac ttggggagcg gaaatggttc cgcgtcttac
     1201 ccgtttttag gagttcgtta agtggcaaag aaaattatcg gctatattaa actgcaaatt
     1261 cctgcaggta aagccaatcc atctcctccg gttggtcctg ctttgggtca gcgcggtttg
     1321 aatattatgg aattttgtaa ggcatttaat gctgcaaccc aaggtatgga gcctggctta
     1381 ccgattccgg ttgtgattac tgcatttgca gataaatcat tcacatttgt gatgaaaacc
     1441 ccgccagctt ctatcttgtt gaaaaaggct gccggtttgc aaaaaggtag ttctaatcct
     1501 ctgaccaaca aagtgggtaa attgacccgt gcccagttgg aagaaattgc taaaactaaa
     1561 gagcctgatt tgactgctgc tgacttggat gcggctgtcc gtactatagc aggttctgct
     1621 cgctcaatgg gcttggatgt ggagggtgtt gtataatggc taaagtatct aaacgcttga
     1681 aagctcttcg ctcttctgtg gaagccaata aattatatgc aattgatgaa gcaattgctt
     1741 tggtaaaaaa agcagcgact gctaaatttg acgagtctgt tgacgtatct ttcaacttgg
     1801 gcgttgatcc gcgtaaatct gaccaagtta tccgtggttc ggtcgttctg cctaaaggca
     1861 ccggtaagac aacccgtgtg gctgtattta ctcaaggtgc aaatgcagaa gctgctaaag
     1921 aagctggtgc agatatcgtc ggtttcgaag atttggctgc tgaaatcaaa gcaggcaatc
     1981 tgaactttga cgttgttatt gcttctcccg atgcaatgcg tattgttggt caattgggta
     2041 ccatcttggg ccctcgtggt ctgatgccaa accctaaagt aggtacggtt acccctaatg
     2101 ttgccgaagc agtaaaaaat gcaaaagcag gtcaagtgca gtaccgtacc gacaaggcag
     2161 gtattgttca tgcaacgatt ggtcgtgctt cattcgctga agctgatttg aaagagaact
     2221 ttgatgcgtt gctggatgct atcgttaaag ccaagcctgc tgccgctaaa ggtcagtatc
     2281 tgaaaaaagt tgctgtgtct agcaccatgg gtttgggtat tcgcgttgat acatcaagcg
     2341 taaataacta atcttaagga attttcaagc agtttggttt tctgggctgc ttgaatttgg
     2401 gctacttaaa attaagtaga tgtccaagac cgtagggatc gtaagattta atcgtaactg
     2461 ccctacgcag acggtagtcc tgaaacacat tgcaagattg cttgtaagat gtctttttag
     2521 gttaccgcgc tggtgggata tcgttttggt atcctgttta taaacagtgg gaggtagacc
     2581 ttgagtctca atattgaaac caagaaagtg gcggtcgagg aaattagcgc ggcaattgct
     2641 aatgctcaaa ccctcgtagt cgctgaatat cgcggtatca gtgtttccag tatgactgag
     2701 cttcgtgcga atgcacgtaa agaaggcgtt tatttgcgcg ttctgaaaaa tactttggct
     2761 cgtcgtgcag tgcaaggtac ttcatttgca gaattggccg atcaaatggt tggtccgttg
     2821 gtttacgctg cttctgaaga tgctgttgct gctgctaaag tgttgcacca attcgcgaaa
     2881 aaagatgaca aaattgtcgt taaagccggt tcttacaatg gcgaagtaat gaatgctgct
     2941 caggttgctg agttggcttc tattccgagc cgcgaagagc tgttgtccaa actgttgttc
     3001 gttatgcaag ctcctgtatc gggctttgcg cgcggtttgg ctgctttggc agagaaaaaa
     3061 gccggcgaag aagccgctta atcgattttg tttctgttaa tcaattattt tttaatacaa
     3121 tatttggagt aaaatagcat ggctattact aaagaagaca ttttggaagc agttggttct
     3181 ttgaccgtaa tggaattgaa cgacttggtt aaagcttttg aagaaaaatt cggtgtttct
     3241 gctgctgctg ttgcagttgc aggtcctgct ggtgccggtg ctgccgatgc tgaagaaaaa
     3301 accgaatttg atgtcgtttt ggcttctgcc ggcgatcaaa aagtcggcgt gattaaagtt
     3361 gtccgtgcaa ttaccggttt gggtctgaaa gaagctaaag acatcgttga cggtgcacct
     3421 aaaaccatta aagagggtgt ttctaaagct gaagccgaag acatccaaaa acaactggaa
     3481 gaagccggcg ctaaagtcga aatcaaataa tttgatgctt cttatgaagg ctggcagttt
     3541 tctgccagcc ttattttgct tcttaaaata agcattaagt attgtttaca tttatttgca
     3601 tagtttttat caaatcattg caaataaatg taaacatcag attgatgcgt gccgttgctt
     3661 tcagacggcc tattattgaa aattactttt cggagtgtat atgagctatt cgtttaccga
     3721 gaaaaaacgt atccgtaaga gttttgcaaa gcgggaaaat gttttggaag ttcctttcct
     3781 gctggcaacc caaattgatt cttatgcgaa gtttttgcag ctggaaaatg cttttgacaa
     3841 acgtaccgat gacggtctgc aggcggcatt taattctatt ttcccgattg tgagccataa
     3901 cggttatgcg cgattggagt ttgtgcatta cacattgggc gagcctttgt tcgatattcc
     3961 cgaatgtcag ttgcgcggaa tcacttatgc agcccccttg cgcgcgcgta tccgtttggt
     4021 gattttggat aaggaagcat ctaaaccgac ggtaaaagaa gttcgtgaaa acgaagtgta
     4081 tatgggcgaa attccgttga tgaccccgag cggttctttt gtgattaacg gcacagagcg
     4141 tgtgattgtc tcccagttgc accgttcgcc cggtgtattc ttcgagcatg acaaaggtaa
     4201 gacgcactct tccggcaaat tgttattctc cgcccgcatc attccctacc gtggttcatg
     4261 gttggatttt gaatttgatc cgaaagattt gctgtatttc cgtatcgacc gccgccgtaa
     4321 aatgccggta acgattttgt tgaaggcttt gggctacaac aatgagcaaa tcttggatat
     4381 tttctacgac aaagaaacgt tctatttgtc ttcaaacggt gttcaaaccg atttggtcgc
     4441 agaccgtctg aaaggcgaaa ctgccaaggt cgatatcttg gataaagaag gcaatgtatt
     4501 ggttgccaaa ggtaagcgca ttactgcgaa aaatatccgt gatattacca atgcaggcct
     4561 gacccgtttg gatgtagaac cggaaagcct gctgggcaaa gcattggctg ccgatctgat
     4621 tgattcggaa accggcgagg tattggcttc tgccaatgat gaaattacag aagagttgtt
     4681 ggccaaattt gatatcaacg gcgtaaaaga aattacgacc ctttatatca atgagctgga
     4741 tcagggtgct tatatctcca ataccttgcg tacggatgag actgccggcc ggcaggcggc
     4801 tcgtgttgcg atttaccgta tgatgcgtcc gggcgaaccg cccaccgaag aggcggtcga
     4861 gcaattgttt aaccgcttgt tcttcagtga agacagctac gatctgtccc gcgtaggccg
     4921 tatgaaattt aatacgcgca catacgaaca aaaactgtcc gaagcccaac aaaactcttg
     4981 gtacggccgc ctgctgaacg aaacgtttgc cggtgctgcc gacaaaggcg gttatgtcct
     5041 gagcgtcgaa gatattgtcg cctcgattgc gactttggtc gagttgcgta acggccatgg
     5101 cgaagtggac gatatcgatc acttgggcaa ccgccgagta cgttcggtag gcgagctgac
     5161 tgaaaaccaa ttccgtagcg gtttggcccg tgtggaacgt gccgtaaaag aacgtttgaa
     5221 tcaggcggaa tcagaaaact tgatgccgca cgatttgatt aatgcaaaac ctgtttctgc
     5281 cgctattaaa gaattcttcg gctccagcca attgagtcag tttatggatc agaccaaccc
     5341 cttgtctgaa gtaacccata aacgccgtgt atctgcattg ggtccgggcg gtttgacccg
     5401 cgaacgtgca ggatttgagg tgcgggacgt gcatccgacc cactacggtc gcgtatgtcc
     5461 gattgaaacg cctgaaggtc cgaacatcgg tttgatcaac tcattgtccg tttatgcgcg
     5521 caccaatgat tacggtttct tggaaacgcc ttaccgccgc gttatcgacg gcaaagtaac
     5581 tgaagaaatc gactacttgt ctgccatcga agaaggccgc tatgtgattg cacaggcgaa
     5641 tgccgatttg gacaaagacg gcaatctgat tggcgacttg gttacttgtc gtgaaaaagg
     5701 cgaaaccatt atggcaacgc ctgaccgcgt tcaatatatg gacgtggcaa ccggtcaagt
     5761 ggtatccgtt gcagcatccc tgattccgtt cttggaacac gatgacgcga accgcgcatt
     5821 gatgggtgcg aatatgcaac gtcaggcagt gccttgcttg cgtcctgaaa aaccgatggt
     5881 cggtaccggt atcgagcgtt ccgttgccgt tgactctgct actgcaatcg ttgcccgccg
     5941 aggcggcgtg gtcgagtatg tcgatgccaa ccgtgttgtg attcgtgtcc atgacgacga
     6001 agcgactgcc ggcgaagtgg gtgtcgatat ttacaacttg gttaaattta cccgttccaa
     6061 ccagtctacc aatatcaatc agcgtcctgc cgtcaaagcc ggcgatgttt tgcaacgcgg
     6121 cgatttggtg gccgatggcg cgtccaccga tttgggcgaa ttggctttgg gtcaaaatat
     6181 gaccatcgcc ttcatgccgt ggaacggtta caactacgaa gactcgattc tgatttccga
     6241 aaaagtggct gcggacgacc gctatacttc gattcacatt gaggaattga atgtcgttgc
     6301 ccgcgatacc aagctgggtg cggaagacat tacccgcgat attccgaact tgtccgagcg
     6361 tatgcaaaac cgtttggacg aatccggtat cgtttacatc ggtgcggaag tagaagccgg
     6421 cgatgtgttg gtaggcaagg taacgcctaa aggcgaaacc cagctgacgc cggaagaaaa
     6481 actgctgcgc gccatcttcg gtgaaaaagc atctgacgta aaagatactt cattgcgtat
     6541 gcctaccggc atgagcggta ccgttatcga cgttcaagtc ttcactcgtg aaggtattca
     6601 acgcgacaaa cgtgctcaat ccattatcga ttccgagttg aaacgctacc gtttggattt
     6661 gaacgaccaa ttgcgtattt tcgacaacga cgcattcgac cgtatcgagc gtatgattgt
     6721 cggtcagaaa gccaacggcg gcccgatgaa gctggccaaa ggcagtgaaa tcacgaccga
     6781 atatctggcg ggtctgccga gcaggcacga ttggttcgat atccgtctga ccgatgaaga
     6841 tttggccaag cagttggaac tgattaaagt gagcctgcaa caaaaacgcg aagaagcgga
     6901 cgagttatac gaaatcaaga agaaaaaact gacccaaggc gacgaattgc aacccggcgt
     6961 acaaaaaatg gtgaaagttt ttatcgccat caaacgccgt ctgcaagccg gcgacaaaat
     7021 ggcgggccgc cacggtaaca aaggcgtggt atcgcgcatt ctgccagtgg aagacatgcc
     7081 ttacatggcg gacggccgtc cggtagacat cgtactgaac ccattgggcg taccttcccg
     7141 tatgaacatc ggtcagattt tggaagttca cttgggttgg gcagcaaaag gtatcggcga
     7201 gcgtatcgac cgtatgctga aagagcaacg caaagcaggc gagttgcgcg agttcttgaa
     7261 caaactctac aacggcagcg gcaagaaaga agatttggat gccctgactg atgaagaaat
     7321 catcgaactg gcctccaacc tgcgcaaagg tgcatctttc gcctctcctg tattcgacgg
     7381 tgcgaaagag tctgaaatcc gcgaaatgtt gaacttggct tatccgagcg acgatcctga
     7441 ggttgaaaaa ctgggcttca acgacagtaa aacccaaatc acgctgtatg acggccgttc
     7501 aggcgaagca tttgaccgca aggttacagt aggtgtgatg cactatctga aactgcacca
     7561 cttggttgac gaaaaaatgc acgcgcgttc taccggtccg tacagtctgg ttacccagca
     7621 gcctttgggc ggtaaagccc agttcggcgg ccaacgtttc ggtgagatgg aggtttgggc
     7681 actggaagca tacggcgcag cctacacgct gcaagagatg ctgactgtga agtctgacga
     7741 cgtgaacggc cgtaccaaaa tgtacgaaaa catcgtcaaa ggcgaacaca aaatcgatgc
     7801 cggtatgccc gagtccttca acgtattggt caaagagatt cgctcactgg gcttggatat
     7861 cgatttggaa cgttactaaa caaaagtttt cagacggcct ttcagggtcg tctgaaaaag
     7921 tggtttcaga ataagaatga agcaatcggc atttaggccg tctgaaatca aaagtaccgt
     7981 ttccccatat cgaaaatccg ccatgcggta aaaatacttc cttcaaggag caaaaatgaa
     8041 tttgttgaac ttatttaatc cgttgcaaac tgccggcatg gaagaagagt ttgatgccat
     8101 taaaatcggt attgcctctc ccgaaaccat ccgctcatgg tcttatggcg aagttaaaaa
     8161 acctgaaacc atcaactacc gtacgttcaa acctgagcgt gacggtttgt tctgtgccaa
     8221 aatctttggc ccggtcaaag actacgaatg cttgtgcgga aaatacaaac gcttgaaatt
     8281 taaaggcgta acgtgtgaaa aatgcggcgt ggaagtaacc ctgtccaaag tgcgccgcga
     8341 acgcatgggc catatcgaat tggctgcgcc cgtcgcacac atttggttct taaaatccct
     8401 gccttcccgc ttgggtatgg tactggacat gactttgcgc gatatcgaac gcgtattgta
     8461 ctttgaagca tttgtggtaa ccgatcccgg tatgactccg ctgcaacgcc gccaattgct
     8521 gactgaagac gattactaca acaagctgga cgaatacggc gacgatttcg atgccaaaat
     8581 gggtgcggaa ggtatccgcg aattgctgcg taccctgaat gtagcgggcg aaatcgaaat
     8641 cctgcgccaa gagttggaat cgaccggttc cgacaccaaa atcaaaaaaa tcgccaaacg
     8701 cttgaaagta ttggaagcct tccatcgttc cggtatgaaa ctggaatgga tgattatgga
     8761 tgtgctgccg gtattgccgc ctgatttgcg tccgttggtt ccattggatg gtggtcgttt
     8821 tgccacttcc gatttgaacg atttgtaccg ccgcgttatt aaccgtaaca accgtctgaa
     8881 acgtctgttg gaactgcatg cgcctgacat catcgtccgc aacgaaaaac gtatgttgca
     8941 agaagcagtt gactcgctgt tggataacgg ccgtcgcggt aaagccatga ccggtgccaa
     9001 caaacgtccg ctgaaatcat tggcagacat gattaaaggt aaaggcggcc gcttccgtca
     9061 aaacctgctg ggtaaacgtg tggactactc cggccgttcc gtgattaccg taggtccata
     9121 cctgcgcctg caccaatgtg gtctgccgaa aaaaatggct ttggagttgt tcaaaccatt
     9181 cattttccac aaattggaaa aacaaggttt ggcttctacc gttaaagctg cgaaaaaact
     9241 ggtagaacaa gaagtacctg aagtatggga tatcttggaa gaagtgatcc gcgaacatcc
     9301 gattatgctg aaccgtgcgc caaccctgca ccgtttgggt attcaagcgt tcgaacctat
     9361 cctgattgaa ggtaaagcga ttcagttaca cccattggtg tgtgccgcat ttaacgccga
     9421 ctttgacggt gaccaaatgg ctgtacacgt tccattgagc ttggaagcac aaatggaagc
     9481 gcgcaccctg atgctggctt caaacaacgt attgtctcca gccaacggcg aaccgattat
     9541 cgtaccttcc caagacatcg tattgggttt gtactacatg acccgcgacc gtatcaatgc
     9601 caaaggcgaa ggcagcctgt ttgccgatgt gaaagaagtg catcgtgcat accataccaa
     9661 acaggtcgag ctgggtacga aaattaccgt acgtctgcgc gaatgggtga aaaacgaagc
     9721 aggtgagttt gaacctgtcg ttaaccgtta cgaaacaacc gtcggccgtg cattgttgag
     9781 cgaaatcctg ccgaaaggcc tgccgtttga atatgtcaac aaggcattga agaaaaaaga
     9841 aatttctaaa ctgattaacg catcgttccg cctgtgcggc ttgcgcgata cggttatctt
     9901 tgctgaccac ctgatgtaca ccggtttcgg atttgcggca aaaggcggta tttccattgc
     9961 cgttgacgat atggaaatcc cgaaagaaaa agcggccttg ctggctgaag ccaatgccga
    10021 ggttaaagaa atcgaagacc aataccgtca aggtttggtt accaatggcg aacgttacaa
    10081 caaagtagtg gatatttggg gtcgtgccgg cgataaaatc gctaaagcga tgatggataa
    10141 cttgtccaaa caaaaagtta tcgaccgtgc cggcaacgaa gtcgatcaag agtcattcaa
    10201 ctccatttat atgatggcgg actccggtgc ccgtggttct gcagctcaga ttaaacagtt
    10261 gtccggtatg cgtggcttga tggcaaaacc tgacggctcg attattgaaa cgccgattac
    10321 ctcaaacttc cgtgaaggtc tgaccgtatt gcaatacttt attgcgaccc acggtgcgcg
    10381 taagggtttg gcggataccg cattgaaaac cgcgaactcc ggttacctga ctcgtcgtct
    10441 ggtagacgta actcaagatt tggtcgttgt tgaagacgat tgcggtactt cagacggctt
    10501 tgtcatgaag gcagtggtac aaggcggtga tgtgattgaa gcattgcgcg atcgtatttt
    10561 gggtcgtgtt accgcgtctg acgttgtcga tccgtcaagt ggcgaaacct tggttgaagc
    10621 cggtacgttg ctgactgaaa aactggtgga tatgatcgac caatccggtg tcgatgaagt
    10681 caaagtccgt acgccgatta cttgtaaaac ccgtcacggc ctgtgtgcac actgttacgg
    10741 tcgtgacttg gcacgcggca aactggttaa cgccggtgag gcagtcggtg tgattgctgc
    10801 acaatccatt ggcgaaccgg gtacccagtt gaccatgcgt acgttccaca tcggtggtgc
    10861 ggcatcccgt gcggcagcag ccagccaagt agaagccaaa tccaacggta cagctcgttt
    10921 cagcagccaa atgcgttacg ttgccaacaa caaaggcgag ttggttgtca tcggccgttc
    10981 ttgtgaagtc gtgattcacg acgatatcgg tcgcgaacgt gaacgtcaca aagtacctta
    11041 cggtgccatc ctgctggtac aagacggtat ggccattaaa gccggtcaaa ccttggcaac
    11101 ctgggatccg catacccgtc cgatgattac cgaacacgca ggtatggtga aattcgaaaa
    11161 cgtggaagag ggtgtaaccg ttgccaaaca aactgacgat gtaaccggtt tgtccacttt
    11221 ggtggtgatt gacggtaaac gccgttccag cagcgcgtcc aaactgctgc gtccgactgt
    11281 aaaattgttg gatgaaaacg gcgtggaaat ctgtattccc ggtacgtcca ctccggtttc
    11341 gatggcattc cccgttggtg cggtgattac cgtacgcgaa ggtcaggaaa tcggtaaagg
    11401 cgacgtattg gcgcgtatcc cgcaagcctc ttccaaaacc cgcgacatta ccggtggtct
    11461 gccgcgcgtt gccgaattgt ttgaagcacg cgtgccgaaa gatgccggta tgttggcgga
    11521 aattaccggt accgtttcct tcggcaaaga gaccaaaggc aagcaacgtc tgattgttac
    11581 tgacgtggac ggtgtagcat acgagacctt gatttccaaa gagaaacaaa ttctggtaca
    11641 cgacggtcaa gtggtaaacc gcggtgaaac catcgtggac ggcgcggtcg atccgcacga
    11701 tattctgcgt ttgcaaggta tcgaagcact ggcacgctac attgtccaag aggtgcaaga
    11761 ggtttaccgt ctgcaaggtg tgaagatttc tgataaacac atcgaagtca tcatccgtca
    11821 aatgttgcgc cgtgtgaaca ttgcggatgc cggcgaaacc gggttcatta ccggagagca
    11881 ggtcgaacgc ggcgatgtga tggcggccaa tgaaaaagct ttggaagaag gcaaagaacc
    11941 ggcgcgttac gaaaacgtat tgctgggtat taccaaagct tccctgtcta ccgacagctt
    12001 catttctgct gcatcgttcc aagaaacgac ccgtgttctg accgaagccg cgattatggg
    12061 caaacaagac gaactgcgcg gtttaaaaga gaacgtcatc gtcggccgcc tgattcctgc
    12121 cggtaccggt ttgacttatc accgcagccg tcatcagcaa tggcaagggg tagagcagga
    12181 gactgctgaa acccaagtaa cggatgaata atcttcggcg cgtttattca atacaaaacc
    12241 gcaagcttta agcttgcggt ttttctttgt ccgattaagg caaaaacaag cgttttcgtc
    12301 attttgaggc gtgtggatta ttccttaggt attttcgggc cggagaccaa cgaggtggcg
    12361 ggtgtcgtcg gtacgtccgg agaccaaaat aactttgcca gggatgttgg tttcggcggt
    12421 caaaaaaagt agcgtcttaa tgttttccat ttaaacaaat gtcgtctgaa acttcagacg
    12481 gcatttcctt taagaaataa atatgaaacc cagaaatctc ttttttgcag gctgcctgct
    12541 gacttcggcg acgtttgccg aggatatcgg cgtacctgtc gaactgatta acgtcggtaa
    12601 tcggattgcg atgccgtctg aaggggaaag cctcgccctc ctgccgtttg ccgaggatgt
    12661 accgccggtt cgcgatgcaa tgccgtctga agttcctaaa agcgcggcag gcggcgatgt
    12721 tcggggtgac cggatgggaa tgccgattaa catcggatga gcgcggcttt atggcataaa
    12781 aaactgtcgt ggaaaggatt tacaccccaa ataaatttcc gttacaacaa gatcaacagc
    12841 aatatgcccg ccttttattc gcgcagcggc aaggaacggt ttgtcagtat agaaaaaacg
    12901 tattgacagt attttcttca gtcgtccgac tgattgtgag ggatgtcggt aaatatttat
    12961 cggcaaacaa gaaaatcatc tttcttcttg tcgttatgct tgactgtctg cttgcaataa
    13021 aaatataatt ccactcttgc cgacatggtg tcggcaagta tttaactcaa caggacgaga
    13081 aaatatgcca actatcaacc aattggtacg caaaggccgt caaaagcccg tgtacgtaaa
    13141 caaagtgccc gcactggaag cctgcccgca aaaacgcggc gtgtgcaccc gtgtatacac
    13201 gactacccct aaaaaaccta actctgcatt gcgtaaagta tgtaaagtcc gcctgaccaa
    13261 cggttttgaa gtcatttcat acatcggcgg cgaaggccac aacctgcaag agcacagtgt
    13321 cgtattgatt cgcggcggtc gtgtaaaaga cttgccaggt gtgcgttacc acactgtacg
    13381 cggttctttg gatactgcag gcgttaaaga ccgtaaacaa gcccgttcca aatacggtgc
    13441 taagcgtcct aaataattac tgggacttaa ataggcacgt cggccgccta agctgaacaa
    13501 cggccgagta agtgaatact caattgggta ttcatgggaa tagacccgac tgaatagatt
    13561 aaaggaaatt aaaatgccaa gacgtagaga agtccccaag cgcgacgtac tgccagatcc
    13621 taaattcggc agcgtcgagt tgaccaaatt catgaacgta ttgatgattg acggtaaaaa
    13681 atccgttgcc gagcgtatcg tttacggtgc gttggaacag attgagaaaa aaaccggcaa
    13741 agtagcaatc gaagtattta acgaagccat tgcaaacgcc aaacctatcg tggaagtgaa
    13801 aagccgccgt gtaggtggtg caaactacca agttcctgtt gaagttcgtc cttcacgccg
    13861 tttggctttg gcaatgcgct gggttcgcga tgcggcccgc aaacgtggtg agaaatccat
    13921 ggacctgcgt ttggcaggcg aattgattga tgcgtccgaa ggccgtggcg gtgcgttgaa
    13981 aaaacgtgaa gaagtacacc gtatggctga agccaacaaa gcattctctc acttccgttt
    14041 ctaattttga aaggctaata aaatggctcg taagaccccg atcagcctgt accgtaacat
    14101 cggtatttcc gcccatatcg atgcgggtaa aaccacgacg acagaacgta ttttgttcta
    14161 taccggtttg acccacaagc tgggcgaagt gcatgacggt gcggctacta ccgactacat
    14221 ggaacaagag caagagcgcg gtattaccat tacctccgct gccgttactt cctactggtc
    14281 cggtatggcg aaacaattcc ccgagcaccg cttcaacatc atcgacaccc cgggacacgt
    14341 tgactttacc gtagaggtag agcgttctat gcgtgtattg gacggcgcgg taatggttta
    14401 ctgcgcggtg ggcggtgttc aaccccaatc tgaaaccgta tggcggcaag ccaacaaata
    14461 ccaagtgccg cgcttggcgt ttgtcaataa aatggaccgt cagggtgcca acttcttccg
    14521 cgttgtcgag caaatgaaaa cccgtttgcg cgcaaaccct gtacctatcg tcattccggt
    14581 tggtgcggaa gacaacttca gcggtgtggt tgatttgttg aaaatgaaat ccatcatttg
    14641 gaatgaagtc gataaaggta caacctttac ctatggcgat attcctgccg aattggtcga
    14701 aactgccgaa gaatggcgtc aaaatatgat tgaagccgca gccgaagcca gcgaagaact
    14761 gatggacaaa tacttaggcg gcgacgagct gaccgaagaa gaaatcgtag gcgcgttgcg
    14821 tcaacgtact ttggcaggcg aaattcagcc tatgctgtgt ggttctgcat ttaaaaacaa
    14881 aggtgttcaa cgtatgttgg acgcagttgt agaattgctg ccagctccta ccgatattcc
    14941 tccggttcaa ggtgtcaacc cgaataccga ggaagccgac agccgtcaag ccagcgatga
    15001 agagaaattc tctgcattgg cgttcaaaat gttgaacgac aaatacgtcg gtcagctgac
    15061 ctttatccgc gtttactcag gcgtagtaaa atccggcgat accgtattga actccgtaaa
    15121 aggcactcgc gaacgtatcg gtcgtttggt acaaatgact gccgcagacc gtactgaaat
    15181 cgaagaagta cgcgccggcg acatcgcagc cgctattggt ctgaaagacg ttactaccgg
    15241 tgaaaccttg tgtgcggaaa gcgcgccgat tatcttggaa cgtatggaat tccccgagcc
    15301 ggtaatccat attgccgttg agccgaaaac caaagccgac caagagaaaa tgggtatcgc
    15361 cctgaaccgc ttggctaaag aagacccttc tttccgtgtc cgtacagacg aagaatccgg
    15421 tcaaaccatt atttccggta tgggtgagct gcacttggaa attattgttg accgtatgaa
    15481 acgcgaattc ggtgtggaag caaatatcgg tgcacctcaa gtggcttacc gtgaaactat
    15541 ccgcaaagcc gttaaagctg aatacaaaca cgcgaaacaa tccggtggta aaggtcaata
    15601 tggtcacgtt gtgattgaaa tggaacctat ggaaccgggg ggcgaaggtt acgagtttat
    15661 cgatgaaatt aaaggtggcg tgattcctcg cgaattcatt ccgtctgtcg ataaaggtat
    15721 ccgcgataca ttgcctaacg gtatcgttgc cggctatcct gtagttgatg tacgtatccg
    15781 tctggtattc ggttcttacc atgatgtcga ctcttcccaa ttggcatttg aattggccgc
    15841 ttctcaagcg tttaaagaag gtatgcgtca agcatctcct gccctgcttg agccgattat
    15901 ggcagttgaa gtggaaactc cggaagaata catgggcgac gtaatgggcg acttgaaccg
    15961 ccgtcgcggt gttgtattgg gtatggatga tgacggtatc ggcggtaaaa aagtccgtgc
    16021 cgaagtacct ctggcagaaa tgttcggtta ttcgaccgac ctgcgttctg caacccaagg
    16081 ccgcgctact tactctatgg agttcaagaa atattctgaa gctcctgccc acatagctgc
    16141 tgctgtaact gaagcccgta aaggctaatc aggcaaatag gccgtccgaa aggctgaaat
    16201 gatttttcag acggcattgt tctttaatcg atctttaatg taaaggaatt agc
//
LOCUS       DAXHRI010000033        15700 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXHRI010000033 DAXHRI010000000
VERSION     DAXHRI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.033"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(459..794)
                     /locus_tag="ACFM75_000785"
     CDS             complement(459..794)
                     /locus_tag="ACFM75_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC6255365.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(874..2994)
                     /gene="dnaX"
                     /locus_tag="ACFM75_000786"
     CDS             complement(874..2994)
                     /gene="dnaX"
                     /locus_tag="ACFM75_000786"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC6255366.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPVALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALLSKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPSALAHYDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELKSPSAQTAEKETAAK
                     KPQPRPEAETTQTPVQTASAAAMPSEGKAAEPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETSPDNQVSKNKAADNETETSLSEVPSENPIQATPNDEAVETEAF
                     AHEAPAEPFYGYGFPDNDCPPEDGAEIPPPDWEHAAPADAEEENNADEGSNDENNTPS
                     APPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATADK
                     KRLDKIRDTLAQAYGLQLTLQTQDWHDESGRETPAMQDKRVQAEDRQKAQALLEADPA
                     AQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1766..1805)
                     /gene="dnaX"
                     /locus_tag="ACFM75_000786"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(3089..5065)
                     /gene="mutL"
                     /locus_tag="ACFM75_000787"
     CDS             complement(3089..5065)
                     /gene="mutL"
                     /locus_tag="ACFM75_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC6255367.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELDGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMIERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGEDFQT
                     ASLGIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSVSNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADHADALAGFGLELSDMGGNTLAVRTVPAML
                     GKADVVSLAKDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            5208..5393
                     /locus_tag="ACFM75_000788"
     CDS             5208..5393
                     /locus_tag="ACFM75_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC6255368.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5404..6072
                     /locus_tag="ACFM75_000789"
     CDS             5404..6072
                     /locus_tag="ACFM75_000789"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6255369.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLMRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPKHGRITACDINVSFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            6072..6788
                     /locus_tag="ACFM75_000790"
     CDS             6072..6788
                     /locus_tag="ACFM75_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC6255370.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGRIVRLSNEVETLNGKVKALEHAKTHPSGRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTAETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            6836..7321
                     /gene="purE"
                     /locus_tag="ACFM75_000791"
     CDS             6836..7321
                     /gene="purE"
                     /locus_tag="ACFM75_000791"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC6255371.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7504..8958)
                     /locus_tag="ACFM75_000792"
     CDS             complement(7504..8958)
                     /locus_tag="ACFM75_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC6255372.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8955..9656)
                     /locus_tag="ACFM75_000793"
     CDS             complement(8955..9656)
                     /locus_tag="ACFM75_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC6255373.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNIE
                     PIVFEREIDTWKTEFFTNINAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9653..10432)
                     /locus_tag="ACFM75_000794"
     CDS             complement(9653..10432)
                     /locus_tag="ACFM75_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC6255374.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10716..12122
                     /locus_tag="ACFM75_000795"
     CDS             10716..12122
                     /locus_tag="ACFM75_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6255375.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADM
                     NESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGNDTALDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            12234..13760
                     /locus_tag="ACFM75_000796"
     CDS             12234..13760
                     /locus_tag="ACFM75_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6255376.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVFRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            14265..15569
                     /locus_tag="ACFM75_000797"
     CDS             14265..15569
                     /locus_tag="ACFM75_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC6255377.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT         3170 a         4168 c         4446 g         3916 t
ORIGIN      
        1 gttttttccg atagattccc gtggtttttc ggttactaga ttcccgcgtt cgcgggaatg
       61 acggcggcgg ggggttctgt tttttccgat agattcctgt ggtttttcgg ttactggatt
      121 cccgcgttcg cgggaatgac ggggtgtaag tttctgctcc cacggggctg gattcccgtt
      181 ttcatgggaa tgacgaaatt tcagacggca tttaagcggt acggatgtgt aaataatggt
      241 agggtgggct tcagcctgcc gattcccgct attcttgccg tttttgcgtt cttatcattc
      301 tcactgtttt taccgttcac gccgaatcct gcaaactgat gccgtcattc ccgcgaaggc
      361 gggaatctag acccgtcggc acggaaattt atcgagtaaa acggtttctt agattctacg
      421 ttctagattc ccgcctgcgt gggaatgacg gtcgggtatc agcggaagaa gtcgcccact
      481 ccggggggta gaccttgcgt gaatgcgccc attgttttgt ttgcggtttc ttcggctttg
      541 tcacgggcgg atttgagggc ggcgaggatg aggtcttcga gcatttcttt gtcgtcggcg
      601 gcttcttgaa tcaaatcggg gctgatgtcg attttgcgta cttcgtgcgc gcaggtcatt
      661 gtgattttga ccaagccgtt gcctgcttcg ccttcgattt cggtttcggc aagtttggct
      721 tgcgcttttt tcatattttc ctgcatttgc tgcgcctgtt tcatcaggcc gcctaatccg
      781 gcttttccga acatactgaa tactcctgtc tgttttgatt gaataaggga aacggcaatg
      841 ccgtccgaag ggttcgggcg gcattatatc tgtttatggc cggtttgccg ccaattccag
      901 tgattcgggc tgccattgcg cgccgaatgc ttggaggatt ttttgtgcgg cggggtcggc
      961 ttcgagcaat gcttgtgctt tttgcctgtc ttcggcttgg acgcgcttgt cctgcattgc
     1021 gggggtttct ctgccggatt cgtcgtgcca gtcttgggtt tgcagggtga gttgcagccc
     1081 gtaggcttgg gcaagggtgt cgcggatttt gtcgaggcgt tttttgtcgg cggtggcgcg
     1141 tgcttcggcg gtcattgcca aaaccatcag gccggtgtcg ggatggtatt ccgtccacgc
     1201 ggagtgttgc gccggcattt gcgccgcgcc gagtttgcgg gcgaaatgcc ggacgatggc
     1261 tgcccagttt tcggtggaaa actcgggcgg cggggcggac ggcgtgttgt tttcgtcgtt
     1321 gctgccttcg tcggcgttgt tttcttcttc cgcatcggca ggggcggcgt gttcccaatc
     1381 cggcggcggg atttctgcgc cgtcttccgg ggggcagtca ttatccggaa agccgtaacc
     1441 gtagaaaggt tctgcagggg cttcgtgcgc aaatgcttcc gtttcaacgg cttcatcatt
     1501 cggtgttgcc tgaatggggt tttcagacgg cacttcggac aaggaagtct cggtttcgtt
     1561 gtcggctgcc ttgttcttgg aaacttgatt gtcaggcgat gtttcggctt cggatgccgt
     1621 ctgaatgctt tttgccgacg tttgcgccgt gccggctgcg gcttgggctt cgtccggtgc
     1681 gtcttcccaa ggcggaacat cgttgttttc ttgattggaa acgggttcgg cagctttgcc
     1741 ttcagacggc attgccgctg cggatgccgt ctgaacgggc gtttgggtgg tttctgcttc
     1801 agggcgcggt tggggctttt ttgcagcggt ttccttttcg gcggtttgtg ccgatgggga
     1861 ttttagttcg gtattttcaa tcacggcgtt tgcatcacac gaagctgccg ccaagggcgc
     1921 aaacgccagc atacgcagca gggtcatcat aaagccggcg tattcgtcgg gggcgaggct
     1981 caggtcgcgt ttgccgtgga cggcgatttg gtagtaaagc tggatttgtt cgccgcttat
     2041 ggtttgggcg aggcggtgca aaatatcgga atcggggtcg tagtgcgcca aggcggaggg
     2101 caccgcctgt atcagggcga ggtgttgcag cagtatggca agttcgccca aggcgttgtc
     2161 aaagccgacg gcacacgccg ccatttcctg cgctttggag agcagggctg cgccgtcttg
     2221 gttgatgatg cctgtcaaga gttcgtaaag gtattgtttg tcaaccgcgc cgatcatttg
     2281 gcggacatcg ttttcggcaa ctttgcccga acctagggcg atggcttggt cgagcaggct
     2341 caaggcatcg cgcatcgatc cggcggcggc gcgtcccaaa agttgcaggg cgacgggttc
     2401 gtaggcgatt ttttcgctgt cgaggacgtg ggcgaggtgg tcggcaacct gctgcgcagt
     2461 catattgcgt aagacaaatt gcaggcagcg gctcaagacg gtaacgggga ctttgtgcgg
     2521 atcggtcgta gcaaggatga atttgacgtg ttcgggtggc tcttccagcg ttttgagcat
     2581 agcgttgaac gcgcttttgg aaagcatatg cacttcgtcg atgatataga ctttgtattt
     2641 gccggcggtc ggcgcgtatt gggcgttttc caatacttcg cggatgttgt cgatgcctgt
     2701 gttggaagcg gcatcgattt ccaataaatc tacatagcgt ccggcatcga tttgggtgca
     2761 gctttcgcac acgccgcaag gttcgccgtg ttgcgcgttt tcgcagttga ggcttttggc
     2821 aaggatgcgg gcaatggtgg ttttgcctac gccgcgcgta ccggtcagca ggtaggcgtg
     2881 gtgcagcctg ccttcgtcca aggcattttg cagggctttg acgacgtgtt cctgaccgac
     2941 taggtcggaa aaggttttgg gccgccattt tcgggcgaga acttgatagg ccatgttttt
     3001 ctcttggttt cggtcgtgat gtttctgtcg gtgcgtcgga atgccgtctg aacggcggct
     3061 tcgggcggcg tattctagca ctttcggctt actgtccgcg caagaacagt gcgtccaatt
     3121 ctttcaaagt cagtttgacc caagttggcc tgccgtggtt gcactggttg ctgcgcggcg
     3181 tgttttccat atcgcgcaga agggcgttca tttcgggcag ggtaagccgg cggccggcgc
     3241 ggatcgagcc gtggcaggac atagtggcga ggatgcggtt ttcgtgttct tcgatggttt
     3301 ggctgctgcc gacttgggcg agttcgccca atacgtcttt ggcgagtgag acgacgtcgg
     3361 ctttgccgag catggcggga actgtgcgga cggcaagggt attgccgccc atgtcggaca
     3421 gttccaagcc gaagcctgcc agtgcatcag catggtcggc aagggcggcg cattcttcgt
     3481 gggacgcggc aaaggttacg ggaataagca ggcgttggct ttgcagcctg ccgttttcct
     3541 gacgttggcg tttcattttt tcgtagttga cgcgttcggc ggcggcgtgc atatcgatga
     3601 gcagcaggct gtcttcggct tgggcaagaa tgtagatgcc taatagttgg gcaatggcaa
     3661 aaccgagcgg cggcagttcg gattgggacg ggatgccgtc tgaaagcggc gtatctgttt
     3721 ggggagcagg cgtttcagac ggcatattgc cgaaacgtgc ctgctcgaat cggcttaact
     3781 caaggtcgat gtcgtcggtt tttttgtaaa gttcggcgta agtattcatt gccgcgcggc
     3841 tttcgcgcag ggacaggctg cgttgttgcg gcgcatatgc ggactgatag ggcatgggcg
     3901 cggttttgcc tgatgaacca aaggcattgt gtgtatctga tttgttgcct gtcgggtagt
     3961 tggatacgct atcaaacaga ttttcgctgt cgttttcaga cggcattggg gtggagacaa
     4021 cgccggtaat gtcatgcaac acttcgcctg cgttgccgac gctttcggtc aggttggcgc
     4081 gtgtgtcggc aagggctttg ttgagcgtgt ggaacacaag ttggtgcacc tgctgactgt
     4141 cgcggaagcg gatttcggtt ttggtcgggt ggacgttgac atccacggct tcgggcggca
     4201 ggtcgaggaa gaggacgaaa gcaggcgtca gcgcgttgtg caatacgtct cggtatgcct
     4261 gcttgacggc atggagcatg actttgtcgc gcacgaagcg gtggttgacg aagcagtatt
     4321 gtttgtcggt tttacctttg gcgaaagtcg gtttggcaat cgcaccatag agccgcagcg
     4381 cgccgttgcc gctgtcgatt cccaatgatg ccgtctgaaa gtcttcgccg acaatggcgg
     4441 caatccgttc atgcaggctt tgtgcaggga gtttgaacac ttgtttgccg tcgcgtttga
     4501 gcgagaaggc aatgtgcgga tgcgccagcg cgaggcgttc gatcatggtg gcgcagtggg
     4561 cgtattctgt attttcggat ttgaggaact tgcgccgcgc gggggtgttg aagaagagtt
     4621 cggcagcttc gatggtggtg ccgacggggt gggcggcggc ggtggggctg ctgagtttgc
     4681 cgtcttcggc tttgacttgg gttgcgtgcg aactgtcttc ttgacggctg gtcagggtca
     4741 ggcggctgac ggaggcgatg cttgccaagc cttcgccgcg aaagcccatg ctggcgacgt
     4801 gttccaagtc gttcagggtt ttgattttgc tggtggcgtg gcggtggagc gcgagttcga
     4861 tgtcgtccgg atggatgccg ctgccgttgt cgctgacgcg aatcaggcgg atgccgccgc
     4921 cgtccagctc gacatcaatc gccgttgcgc ctgcatcgat actgttttca acgatttctt
     4981 tcaaggcgtt ggcagggcgt tcgactactt cgccggcggc gatttggttg acgagatggt
     5041 cgggcagggc ggcaatgcgt ggcataaggt aggcttccgt tgcaaaaacg ctttattcta
     5101 taataaaacc ccttactttt ctgcctgtat tcttgataaa agccgtttat cccgtttgga
     5161 aaataccagt ataatcacgc catattccgt aaaattggag cacaaagatg tatcactacc
     5221 aatccgatgc cacacaattc ctcaaccgcc tgattgaaga aaaacctgag ttggaacagc
     5281 agcgtttgga aaacaggggg cttttgtggg atgtcgaact caatcccgaa gaacaggaaa
     5341 acttcgaggc ggcaaaagta gcgaaaaaac cttataccta ttaccaagac tgaacccgcc
     5401 gaaatgacga cccatctgtc caatgtcgcc cccgacctgc aaaactattt gaacgccatc
     5461 ggcgaacctg aacatcccgt tttgatgcgg ctgcgcgaga agaccgggca tcaccgtatg
     5521 ggcaaaatgg cgattgcgcg cgaacaggcg gcggttttgg tttggctggc aaagctgatc
     5581 cgtgcggaaa aatatctgga aatcggcgtg tttaccggat acagcagcac cgcgcttgca
     5641 ttggcgttgc ccaaacacgg gcggattacc gcctgcgaca tcaatgtaag ctttaccgat
     5701 acggcgcgtc aggtttggaa cgaggccggt gtggcacata aaatcagcct gcacctgcaa
     5761 cccgcattgc tgacattgga tgatttgatt gcacagggtg aagccggaag ctacgatttg
     5821 gcactgatag acgcagacaa accgcccacg ccgcaatatt tcgagcgttg cctcaagctt
     5881 gtccggcaag gcggcatcat tgccatcgac aatattttgt tgaacggaag ggtgatgcgc
     5941 gaagcggctt ccgatgcgcc gcccagcgtc ggcatcctca aagattttaa tcaaaacctg
     6001 ccgaacgata cgcggattgt ccccatcacc ctgcccgtcg gcgacggttt gactctgctt
     6061 ctgaaaaaat aatgaagacc aaattaccgc tttttatcat ttggctgtcc gtatccgccg
     6121 cctgttcttc ccctgtttcc cgcaatattc aggatatgcg gcccgaaccg caggcagagg
     6181 caggtagttc ggacgctatt ccctatcccg ttcccactct gcaagaccgt ttggactatc
     6241 tggaaggcag aatcgtccgg ctgtcgaacg aagtggaaac cttaaacggc aaagtcaaag
     6301 cactggagca tgcgaaaaca cacccttccg gcagggcata cgtccaaaaa ctcgacgacc
     6361 gcaagttgaa agagcattac ctcaataccg aaggcggcag cgcatccgca cataccgccg
     6421 aaaccgcaca aaacctctac aatcaggcac tcaaacacta taaaagcggc aggttttctg
     6481 ccgctgcctc cctgttgaaa ggcgcggacg gaggcgacgg cggcagcatc gcgcaacgca
     6541 gtatgtacct gttgctgcaa agcagggcgc gtatgggcaa ctgcgaatcc gtcatcgaaa
     6601 tcggagggcg ttacgccaac cgtttcaaag acagcccaac cgcgcccgaa gccatattca
     6661 aaatcggcga atgccaatac aggttgcagc agaaagacat tgcaagggcg acttggcgca
     6721 gcctgataca ggcttacccg agcagcccgg cggcaaaacg cgccgccgca gccgtgcgca
     6781 aacgatagtt tttccatttt tccgcttttt accccatccg caccaaggag gatttatgat
     6841 acaaatcggc atcatcatgg gcagcaacag cgattggccc gttatgcggc aggcagcgca
     6901 gtttcttgaa gagttcggcg tagaatatga ggcgcgcgtt gtttccgcac accgcacccc
     6961 ggatttgatg ttccaatacg ccgaaaccgc acgggcgcgc ggcatcaaag ccatcatcgc
     7021 gggtgcgggc ggcgcggcgc atttacccgg tatggttgcc gccaagacca ccgtccccgt
     7081 tttgggcgta cccgtcccca gcaaatacct gcgcggcgaa gattcgcttt tatcgattgt
     7141 acaaatgccc aaaggcgtac ccgtcgccac attcgccatc ggcgaggcag gcgcggcaaa
     7201 tgccgcattg ttcgccgttt ccatgctggc caacgaaaac cccgaactgg cgcaaaaact
     7261 ggcagacttc cgagccaaac aggaacaggc tgttttgagt atggaattgg aacagattta
     7321 aaacccgccg tccgcgaata tatagtggct taaatttaaa ccagtacggc gttacctcgc
     7381 cttgccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
     7441 actatacaaa tgccgtctga agccctgttt cgggtttcag acggcatttt tgcgaacaac
     7501 tttttatgcc tgttcttttt ggcgcatttt ttccgccatc agttcgtgca aggttttctt
     7561 cgccggtttc atcggcacgc ggttttgcgt ccaacccaac tgtttgcgcg gggtcaggtt
     7621 gcggaacttg gtggctgccc aaccgaaggc gcggtaggtt ttgctgccgc tgaaaatacc
     7681 gttgaatgtg cgccacgcca tttgttcgcc gaaggtatgc gatgcgcctt gccctcggat
     7741 ggggtgcggc acggtttcgg tcggcgaacg ttgcgcttca acgcgcaaac gctgcatttg
     7801 ttcggtaatc gggatgcgta ccggacagac ttccacacac gcaccgcaca tggtacaggc
     7861 ggttggcagg tcgcgagtgg catccagacc caacaggtga ggggaaataa tctcgccaat
     7921 cgggccggga taggttgtgc cgtatgccgc gccgccgatg cgggtataaa ccgggcaatg
     7981 gttcatacac gcgccgcaac ggatacattg cagggtgcgg cgcatttggt cttcggcata
     8041 agcctggctg cggccgttgt cgagcagaac caagtgcatt tcttgcggac cgtctaattc
     8101 ttcactgcgg cgcgggccgg taatcatatt gaaataggtt gtgatgtttt gaccgatggc
     8161 ggagcgcggc agcagactgt acaagggtgg gatgtcggac aatttcgcca ccactttttc
     8221 aatgccggta atagcgatat gcacgggcgg tacggtggta ctcaagcgac cgttgccttc
     8281 gttttccacc agacacagcg tacctgtttc ggcaacggcg aagttcacgc cgctcaagcc
     8341 gacatcggca gtgctgtaaa tatcgcgcag ggctttgcgg gcgaagccgg tcagttggtc
     8401 cacatcgtct gtaagcggtg tgccgaggtt ttggtggaac agttcgctca cctgttcttt
     8461 ggttttatgg attgcaggca tcacgatatg ggtcggtttt tcgcctgcca tttggacgat
     8521 gaactcgccc aagtcgcttt ctaccgcttt aatgcctttt gcttcaagat aatggttcag
     8581 ctcgatttcc tcgctgacca tcgatttgcc tttgaccatc agcttgccgt ttttggctgt
     8641 gatgatgtcg tggatgattt cgcaggcttc ggccggtgtt tccgcccagt gtacttttac
     8701 gcccaacttg gtcaggtttt cttccaactg ctccaacagc gcgggcaatt tggacaggga
     8761 acgctggcgg atgtgttcgc acaaatcgcg caagctttgc agctcttctt cgtcggtcaa
     8821 aacggctttg cgtttggtca tcagcatatc catcgcggta cgcaggcttt tgcgcaaagg
     8881 cttgtcttga agggaaattg cggcgttttg cttgaaagtt tccggcttca tgtgaaattt
     8941 gatggtttgc gtagtcatgc gttttcctcc aaatcggcag gggaaatgtg gtcgggcagg
     9001 atggcgagga tgaccaaatc gcgcgggccg tgcgcgccgt aagcaagcgt cagttggatg
     9061 tctgcggttt tggacgggcc ggaaatcagg aatacattgg tcggcatacc gttttccacc
     9121 agtttttcgc cttcgacggc attatgaaac tcgttgtaca tcttggacgt atcgaacagg
     9181 cagaaatgca cgggcggaac gagactcaaa gtacgcggtt cttcggggct ggaaaacagc
     9241 atcagcgtgc cggtgcgggc gatgccgcat tgcgcgccgc tgaagcccgc attgatgttc
     9301 gtgaaaaact cggttttcca agtatcgatt tcgcgctcga agacaatcgg ttcgatattg
     9361 ctgcccgcca atgcggcacg ggcaatttgt ccgtgttcgg tcgccaaggg cagcaggatg
     9421 tttttcaaac ccttgccttc tgccgcttcg cggaaaacct gcatccaatt gcttttcgtc
     9481 acccaataaa tttcggtttt gaccgcgcgc attgcggcag cccaatgttt cagacgctcg
     9541 gcttcgctgt cccaagaaac gcccttttcg cggtaataat cgaaaaccgc aggctcttcc
     9601 atcggcaagg catcggcttt tttcagtttt gccaaaatat tttcacgcgc gctcatgctt
     9661 tgcctccggt gcgttccaac aagaaggatg cgatatgttt cggacgcggc atatccggct
     9721 cgtccttggc gattttgccg ccgatgttca tcatacagcc gcagtccgcg ctgatgattt
     9781 cggttgcgcc ggtttctttc agcgcggcga ctttgtcggt taccatcgcg ccggaaatat
     9841 cggcttgttt gacggagaat gtgccgccga agccgcaaca ttcgctttcg tggtcgtgga
     9901 cgatgcgttc gacgttttcc ataccgtcaa tcagttgcca gcctgaaaga tggacattca
     9961 tttcgcggcg ggcggcgcag gaagtgtgaa cggcgacttt gagcggttcg cccttgtctt
    10021 cgggtttgaa accgatggca agcaggaaat gggtaaactc gatgatgcgg ccggcgcaat
    10081 ccacagccct ttcctcgtac tcgctgcctt taaacagcgt cggccaatgg tgtttcatca
    10141 taccgccgca cgaaccggac ggtacgacaa tcggccagtt ttcggggaaa aggtcaagtt
    10201 gcgctttggc gacatcgaag gcttcggtcg gatggccgga tgaataggca ggctggccgc
    10261 agcagctttg cgccatcggg aaatggacgc gtatgccctg ctgctcgatt agggcaatgg
    10321 catccatgcc tgcttcgggc ataaaaagat caaggacgca agtgccgaaa aaatagacat
    10381 cggtcggatt gctgtcgtat cggatgattt ttgggggaat ggttgcgctc atgttttttg
    10441 ataacgggaa acccgttttt ctctgtagaa aggtaagcgt ttactttaag taattgactg
    10501 ttgcgggtca agtctaattt taaaaaataa tccggttttt tttacaaact gccccataac
    10561 gcttactgta ccttactctg ataattttcg ataataatta tcattacaat aaaatgctga
    10621 ttcgtttgct tgtgaacatt caggacgccg actctgacgg cattcagaca gcatctgaaa
    10681 acaataacgg cacaaggatg gggtacaatc gccctatgga aaaaaacacc ctatccgccc
    10741 gcgcaccatc cccttggctg ccgcttctgc tggcaattgc catttttatg cagatgttgg
    10801 atgcgaccat tttaaatacc gcactgcctg aaattgccgc cgacatgaat gagtcgcctc
    10861 tggatatgca actggcagtc gttgcctata cgctgacggt tgccctgctg attcctttga
    10921 gcggttattt ggcggacagg ttcggaacga aaaaagtctt tttcggttcg attgccgttt
    10981 ttatgctcgg atcggcattg tgcgccgcat cgggttcgct gtttgaattg acgctttccc
    11041 gtgtcgttca gggcatcggc ggttcgatgc tggttccgat accgcgtctg accatcttgc
    11101 gtatgtacga caagtccaag ctgctcaatg ctatcaatta tgcggtcatg cctgcattaa
    11161 tcgggccggt tttagggcct ttggcgggcg gttatttggt cgaatacgct tcgtggcact
    11221 ggattttcct gctcaacctg cccatcggtc tgctgggttt catattggga cgcaacatca
    11281 tgcccgatgt caaaggcaac gataccgctc tggatttcaa aggctatctg attttttctg
    11341 ccgccgcgtg cctcttgtta ctttcggcag aaagcctgtc gcacgcgctg cctccgtatt
    11401 ttgcactgtt gccgctgtgc ggcggactgc tgtttgcacg ccgttatttc cgacatatga
    11461 aaaccgcgtc caaaccgatt tattccgccg acctgtttct gatacgcact ttccgtctgg
    11521 gactggcggg caatctgttc agccgtctcg gcatcagctc gattcctttt ctgatgcccc
    11581 tgatgtttca agtcgctttc ggcttcggcg caagcctgtc gggttggctg gtcgcccccg
    11641 tcgccctgtc ttcgctgctg gtcaaaccgc tgattgcacc gctcatgaaa cgtttcggct
    11701 accgcacggt actgctttgg aacaccaagc tgcttgccgc cttcatcatg ctgctcgccc
    11761 tgcctgatgg aaactcgtcg ctgtggattt ggattttcct ctcgctggcg atcggcgcgt
    11821 gcaactccct gcagttttct gccatgaata cactgaccct cgccgatttg cgcccgcaac
    11881 agaccggcag cggcaacagc ctgatggcgg tcaaccaaca gcttgccatc agcatgggca
    11941 ttgttgtcgg cgcattaatc cttaaaaact ggacatttct gataccggct tcttcaggtc
    12001 tgcattccgc cttccgtatg accctgctca gtatcggcgg catcaccctt gcctcgtcgt
    12061 tggttttcaa gcgtctgcac gtttcagacg gtgcaaacct gacacggaac acaccgtcct
    12121 aaagcggtcc gcacgcaaaa cttttacccg tttcaacgtt tggattatga ttccgcactt
    12181 ccatgcgcgc caaccccaaa acacaggcaa tgccgtctga aaccatatcc ctgatgaaaa
    12241 cacgcagcct aatttccctt ttatgcctcc ttctctgttc atgttcttca tggttgcccc
    12301 cactggaaga acggacggaa agccgtcatt tcaatacttc caaacccgtc cgcctggaca
    12361 acatcctgca aatccggcac acccctcata ccaacgggct atccgatatc tatctgctga
    12421 acgaccccca cgaagccttt gccgcccgcg ccgcccttat cgaatctgcc gaacacagcc
    12481 tcgatttgca atactacatc tggcgcaacg acatttccgg ccgactgctg ttcaacctcg
    12541 tgtaccttgc cgcagaacgc ggtgtgcgcg tacgcctgct gttggacgac aacaacacgc
    12601 gcggattgga cgacctcctg ctcgccctcg acagccatcc caatatcgaa gtgcgcctgt
    12661 tcaacccctt cgtcttccgc aaatggcgcg cactcggcta cctgactgac ttcccccgcc
    12721 tcaaccgccg catgcacaac aaatccttta ccgccgacaa ccgcgccacc atactcggcg
    12781 gacgcaatat cggcgacgaa tacttcaaag tcggtgagga caccgttttc gccgacctgg
    12841 atatcctcgc taccggcagc gtcgtcggcg aagtatcgca cgacttcgac cgctactggg
    12901 caagccattc cgcccacaac gccacgcgca tcatccgcag cggcaacatc ggcaagggtc
    12961 ttcaagcact cggatacaac gacgaaacgt ccagacacgc gctcctgcgc taccgcgaaa
    13021 ccgtcgaaca gtcgcccctc taccaaaaaa tacaggcagg acgcatcgac tggcagagcg
    13081 tccaaacccg cctgatcagc gacgaccctg caaaaggact cgaccgcgac cgccgcaaac
    13141 cgccgattgc cgggcggctg caagacgcgc tcaaacagcc cgaaaaaagc gtctatctgg
    13201 tttcacccta tttcgtcccc acaaaatccg gcacagacgc actggcaaaa ctggtgcagg
    13261 acggcataga cgttaccgtt ctgaccaact cgctacaggc gaccgacgtt gccgccgtcc
    13321 attccggcta tgtcaaatac cgaaaaccgc tgctcaaagc cggcatcaaa ctctacgagc
    13381 tgcaacccaa ccatgccgtc cctgccacaa aagacaaagg cctgaccggc agctccgtaa
    13441 ccagcctgca cgccaaaacc ttcattgtgg acggcaaacg catcttcatc ggttcgttca
    13501 acctcgaccc ccgttccgca cggctcaaca ccgaaatggg cgttgttatc gaaagcccca
    13561 aaatcgcaga acagatggag cgcacccttg ccgataccac ccccgaatac gcctaccgcg
    13621 ttaccctcga caggcacaac cgcctgcaat ggcacgatcc cgccacccga aaaacctacc
    13681 cgaacgaacc cgaagccaaa ctttggaaac gcatcgccgc aaaaatccta tccctgctgc
    13741 ccatagaaag tttattatag aaatatagcg gattaacaaa aaccagtacg acgttgcctc
    13801 gccttagctc aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggttccgt
    13861 actattcgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatt
    13921 tccaattaaa aatttttaat atattaatca ataaattaat tttataaaat aaaaatattg
    13981 tcaacaatat tttgccttat cgcccaaacc tctgtatatt ttcctacagt aaattgttga
    14041 caatccatac gcccacatat gcgccgccta aggataaatc ctcccgccgg acaacgggtg
    14101 caagggatcg gatgcgatat ttccatattc aaacaaggga ttggcggcac atcggcaaaa
    14161 tccccgcgcc gccccggtcc ggcagggctt gcgtccctcc cggacaagcc ccgaccccgc
    14221 ctttccgaaa gacgggctca accattaagg aaactttaat caaaatgaaa aaacacatat
    14281 gggcggcatc tttgctgccg gcatccctat cggcagaacc tttaaactgg tggaagcctt
    14341 attccgccgt caattcgggc gataccgcct gggtgatgac tgcggctgcc ttggtactgt
    14401 tgatgacgct tcccgggctg gctttattct acggcggtat ggtgcggaaa aaaaacctgc
    14461 tctcgacgat gatgcacagc ttttccatcg cgacattggt gggaatcctt tgggtcgccg
    14521 tcggctattc tttagcgttc acgccgggaa atgcctttat cggcggtttg gggcgcgtat
    14581 ttttaagcgg gatgcagata gacgctgccg cacagatgct gaccgtgtcg cccaatgcgc
    14641 cgactgttcc cgaaccggta tttatgtttt ttcagatgac gtttgccatt atttcgaccg
    14701 ccattattac tggcgcgttt gccgaacgga tgaaatattc ggcaatgatg ctgttttcgg
    14761 gcatatggtt tttattggtt tatgtgccgg gagcgcattg ggtgtggggc ggcggcttta
    14821 tgagcaaggg cggcgtattg gattatgccg gcggtacggt ggtgcacatc aatgccggta
    14881 tcgcgggact cgtcgccgcc ttggttttgg gcaggcgcat aggctacggg cgcgaggcga
    14941 tgcctccgca caatatggcg atgacactga tcggcgcggc aatgttgtgg ttcggctggt
    15001 tcggctttaa cgccggatcg gcgcttgcgg cagacgcggc ggcgggtatg gcgatggcgg
    15061 taacgcaggt gtcggccgta ttcggcgcgg caggctggct tgcctgcgaa aaaatagcgg
    15121 gacacaaacc ttccgctttg gggctggctt ccggcgcggt ttccggtctg gtcggcatca
    15181 cccctgccgc cggctttacc ggcccgtcgg gcgcggccgc catcggtata ttgactgccg
    15241 ccgcgtgctt tgtgtccgtc accgtcgtca aacacaaatt gcgttacgat gattctttgg
    15301 acgctttcgg catacacgga ttcggcgggc tggtgggcgg aatattgacc ggcatctttt
    15361 tcgacaaccg cattttcggc ggggatgcgg cagtttggca gcagttgtgg atacaggtaa
    15421 aagacgggtt cgttatggcg gcatacagcg ggctgatgag ttgggcgatt ttgaaggtcg
    15481 tggggaaaat ctgcggcggc ctgcgcgtcg gcaaggatgt cgaacgcgaa ggtttggatc
    15541 tgaatatcca cggcgaacgc gtggaataag ggcggttatg ccgtctgaag cctgaaaatc
    15601 gggtttcaga cggcattttt cacgtttgcc gccgatggat aaacatatag tggattaaca
    15661 aaaatcagga caaggcgacg aagccgcaga cagtacaaat
//
LOCUS       DAXHRI010000034        14906 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXHRI010000034 DAXHRI010000000
VERSION     DAXHRI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.034"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="ACFM75_000798"
     CDS             complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="ACFM75_000798"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6255378.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1966..3561)
                     /gene="aceF"
                     /locus_tag="ACFM75_000799"
     CDS             complement(1966..3561)
                     /gene="aceF"
                     /locus_tag="ACFM75_000799"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC6255379.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGTAIIEVETAGSAAAAPAQAAQAAAPAAAPAPTAVAAPAPSAPAA
                     AKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAA
                     KPAAAGASLGSSLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVH
                     EEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLK
                     NYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFT
                     ISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPIWNGKEFAPRLMCPLSLSFDHRVID
                     GAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3711..6374)
                     /gene="aceE"
                     /locus_tag="ACFM75_000800"
     CDS             complement(3711..6374)
                     /gene="aceE"
                     /locus_tag="ACFM75_000800"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC6255380.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYAHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7068..8780
                     /locus_tag="ACFM75_000801"
     CDS             7068..8780
                     /locus_tag="ACFM75_000801"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC6255381.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFKGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            8843..9499
                     /locus_tag="ACFM75_000802"
     CDS             8843..9499
                     /locus_tag="ACFM75_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC6255382.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFAAADRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9509..9964)
                     /gene="ruvX"
                     /locus_tag="ACFM75_000803"
     CDS             complement(9509..9964)
                     /gene="ruvX"
                     /locus_tag="ACFM75_000803"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC6255383.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9957..10505)
                     /locus_tag="ACFM75_000804"
     CDS             complement(9957..10505)
                     /locus_tag="ACFM75_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC6255384.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10507..11067)
                     /locus_tag="ACFM75_000805"
     CDS             complement(10507..11067)
                     /locus_tag="ACFM75_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC6255385.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGKLFAGTCMISNPIENPDKR"
     gene            11395..13200
                     /locus_tag="ACFM75_000806"
     CDS             11395..13200
                     /locus_tag="ACFM75_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6255386.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKSVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13327..14811
                     /locus_tag="ACFM75_000807"
     CDS             13327..14811
                     /locus_tag="ACFM75_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC6255387.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNGETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3534 a         3695 c         4202 g         3475 t
ORIGIN      
        1 gtcggagaag atattttaat ccgctgtaaa atagaaaatg ccgtctgaac atttcagacg
       61 gcatttgctc aaataaacct tatcggctgt ttattcagtc ggatttattt tttcttttgc
      121 ggaggcaggt cggtacaagt acccaatgcc acttccgccg ccataccgat ggattcgccc
      181 aaggtcgggt gcgggtggat ggttttgccg atgtctgccg cgtcgcagcc catttcgatg
      241 gcaaggcaga cttcgccgat catgtcgcca ccgttcggac cgacaatgcc gccgccgatg
      301 atgcggccgg tttcggcatc aaaaatcagc ttggtaaagc cgttgtcgca accgttggca
      361 atcgcacggc cggaagccgc ccacgggaag ttggctttgg tgattttgcg gccggatgct
      421 ttggcggaca actcggtttc gcccacccac gccacttcgg gggaagtgta ggcaacgccc
      481 ggaatcacgc gcgcgtcgaa gtaggctttg tggccggcgc agttttcagc agcaacgtgg
      541 ccttcgtgaa cggctttgtg tgccaacatt ggttgaccga cgatgtcgcc gatggcgtag
      601 atgtgcggca cattggtacg catttgtttg tccacttcga tgaagccgcg atcggtaacg
      661 gcaacgcctg ctttttccgc gctgatgagt ttgccgttgg gcgcgcggcc ggcggcaacc
      721 aatacggcat cgtaacgttg cggctcttta ggcgcatttg caccttcaaa ggtaacgtaa
      781 acgccgtctt ctttcggctc gactgcaacg gttttggtgt tgaccataat gttgtcaaaa
      841 cggtattcgt tttgtttttg ccatactttc accaagtcgc ggtctgcgcc ttgcatcagg
      901 ccgtccatca tttcaaccac atccaaacgc gaacccagcg tgctgtaaac cgtacccatc
      961 tcgaggccga taatgccgcc gccgataatc agcagtttgc ccggtacttc tttcagagcc
     1021 aatgcgccgc tggaatcgat gatgcgcgga tcttcaggaa tgaaaggcag tttggttacg
     1081 cggctgcctg ctgcaatgat acagtttttg aaggcaacga tttttttctc gccggtagtg
     1141 gctgcctgtt cgtacgcgtc gccggcagtc agcgacactt ccaagtggtg cgggtctaag
     1201 aattgcccgt cgccttggat aacgtccact ttacggcttt tcgccatacc tgccaaaccg
     1261 cccgtgaggc gggaaactac gccgtctttg taggcgcgaa gcatatcgat gtcgagttcc
     1321 ggctcggggt atttgatacc gttggcagcc aagtggcgca cttcgtcgat aacggcggca
     1381 ttgtgcaaca aggctttgga agggatacag ccgacgttca ggcaaacgcc gcccaaagtt
     1441 ttgtaacgtt cgacgatggc gactttcaag ccttcatcgg cagcggcaaa tgcagcggag
     1501 taaccgccgg gaccgccacc caatacgacc acgtcgtact cggcatcggc agaaccgccg
     1561 aattgcgcgg cttgcggagc aggagcagca gctttagggg cttcttgcgc cggggcggca
     1621 gccgcttcgg ctttaggagc ggcagccgtg ccttcagctt caacgacaac aatcaaaccg
     1681 ccttcggaga ttttgtcgcc gactttgact ttcacttctt tgaccacgcc cgcaacttcg
     1741 gcaggcacgt ccatcgtcgc tttgtcggtt tccaaggtaa tcagggtgtc gtcaacggcg
     1801 atggtgtcgc ccacgtttac ttcaaccgcg ataatatcta cattttcgtg tccgccaatg
     1861 tcgggcactt tcaattcaac taagctcatg tctcatcttt ctgaatgatg ttcaaacttc
     1921 cttttcagac ggcacagacc gctatgccgt ctgaagccga tcgggttaca gggtaatgcg
     1981 gcggaagtct ttcaacagct tcgccaagaa tacggtaaag cgcataccgg ccgcaccgtc
     2041 gatgacgcgg tggtcgaagg acaggctcaa cgggcacatc aggcgcgggg caaactcttt
     2101 gccgttccaa atcggtttga tttgggattt gcacacgccc aagatggcga cttcgggagc
     2161 gtttacaatc ggcgtgaagc ctgtgccgcc gataccgccc aagctggaaa tggtaaagca
     2221 cgcgccttgc atttcttgcg gtttgagctt gccttcgcgg gcttttttgg acaattcggt
     2281 cagctcttgg ctgatttgtt tcaagccttt ttgatccacg tctttgatga cgggaacaac
     2341 caagccgttc ggcgtatcgg ctgcgaaacc gatgttgaag tagtttttca gcaccaggtt
     2401 gtcgccgtcc agagaagcgt tgaattcggg gaaggctttc agcgcggaaa cggaggcttt
     2461 gatgatgaac gccaacggtg acagtttcac gccttcgcgt tcccattctt tgttcagttg
     2521 tttgcggaat tcttccagct cggtcatatc tgcttcttcg tgtacggtaa cgtggggaat
     2581 caccacccag ttgcgagaca ggttttgacc ggagattttc ttaatgcggg acaattcttt
     2641 aacttcgaca ttgccgaatt tggagaagtc cactttaggc cacggcagca agtccagact
     2701 gctgcccaaa gatgcgccgg ctgcggcagg ttttgccgcg ccaccctgca ttacggattt
     2761 cacaaaggct ttgatgtcgt cgcccacgat acggcctttc aagccggtac ctttgacttg
     2821 gcccaaatcc acgcccaatt cgcgcgccag tttgcgtgcg gaagggccgg cgtgtgcttt
     2881 ggcgaaagcg gcctcgtcga ttttggcagc ggcaggtgca gaaggtgcag gcgcggcgac
     2941 agcagtcgga gccggagcgg cagccggtgc ggcagcttga gcggcttgag caggggctgc
     3001 tgcggcagag ccggcggttt ctacttcgat aatggcagta ccttcggaca ctttgtcgcc
     3061 gacttttaag aatacggctt taacgacacc ggcagcggta caaggtacgt ccatcgttgc
     3121 tttgtcggtt tccaaagtaa tcagcgtgtc gtcttcggca acggtatcgc cgactttgat
     3181 ctcgacggcg attacatcca catcggtatg gccgccgatg tcgggaacgg ctacttgaac
     3241 ggttgcaccg cctgcggcaa cgggtgcggc agcaggtgca ggttgtgctt cggcagcagc
     3301 aggagcggct tcggcagcgg cggcaccggt ttcaacggtc aaaattacgc cgccttcgga
     3361 gattttgtcg ccgactttga ctttaacttc tttcacgaca ccggccgcat cggcaggcac
     3421 atccatcgtg gctttgtcgg tttccaaagt aatcagggtg tcgtcaacgg cgatggtgtc
     3481 gcccgcttta acttctacgg cgatgatgtc gacgttttcg tgaccgccga tatcggggac
     3541 tttgatttct acgatactca tttgagttcc tttaatcttt ttcagttgga tgccgtctga
     3601 aaactgcttc acgcggcatc ggtacggttt gtctgttcaa accattcggg cattcggttt
     3661 tcagacggca tgatgtctga atggggcaaa caggccgtct gaaacatcaa tcagcgtttc
     3721 cagctagggg ctgaatcggc tttgatgccg tatttttcaa tggcttgttg aacagtttct
     3781 ttgctgactt tgccttgttc cgccaatgcg gccaatgctg ctacggcaac gttgtagcga
     3841 tccacttcaa agaagcggcg caggttggcg cgactgtcgg aacggccgaa accgtcagtg
     3901 cccaagacgt ggtagtcgtt agggatgtag gcacggatgc ggtcagcata gctgcggata
     3961 tagtcggtag cggcgataac cggaccgtca tgaccttgca gttgagaggt aacgaaaggc
     4021 actttttcag cttccagcgg atgcaggcgg ttgaagcgtt cggtttcgat ggcgtcgcgg
     4081 tgcagcaggt tgaaggacgg gcaagaccag atgtctgctt ccacgccgaa gtcggctttc
     4141 agcagctcgg caccggcaat gacttcttgc aggatggtac cggaacccat cagttggact
     4201 ttcttgtcgc ctttgccgcc ggctttcagc aggtacatac ctttcaagat gtcttgttcc
     4261 gcaccttcgg gcatatccgg atgggcgtag ttctcgttca tcagggtgat gtagtagaac
     4321 acgtcttcat tattggcata catacggcgc agaccgtctt gtacgatgac ggcgacttcg
     4381 tattggaaag tcgggtcata agatacgcag ttcggaatca ggtcggcctg gatgtggctg
     4441 tggccgtctt cgtgttgcag gccttcgccg ttcagcgtcg tacggccggc agtaccgccc
     4501 agcaggaagc cgcgcgcgtg catatcgccc gccgcccaag ccaagtcgcc gatacgttgg
     4561 aaaccgaaca tagaatagta aatgtagaac ggaatcatgg cgaagttgct gttggcgtag
     4621 ctggttgcag ccgcaatcca gtcggccatc gcgcccggtt cgttaatacc ttcttgcaag
     4681 atttgaccgt caacggattc tttatagaac atcagttggt ctttgtcttg aggggtatat
     4741 tgctgacctt tcggattcca aataccgtat tggcggaaca taccttccat gccgaaagta
     4801 cggctttcgt cgggaacgat aggtacgacg cgtttgccga tttttttgtc tttcagcaaa
     4861 gtggacagga tgcggacgaa tgccatcgtg gtcgagaact cgcgttcacc gctggatttg
     4921 agttgtgcgt cgaatgctga cagctcgggc acttccaata cttcctgcgt cggtttgcgt
     4981 tgcggcaggt agccgcccaa agcatcgcgg cgtgcgtgca ggtatttgta ttcttccgta
     5041 tcgggggcaa aagtcaggta aggcagatcg ccgctttcga tttgttcgtc ggtaaccgga
     5101 atgtcaaagc ggtcgcggaa ttgtttcagg gacgctttgt ccattttttt ggcttggtgg
     5161 gcaacgttct gaccttcgcc ggatgcgccc ataccgtaac ctttaatggt tttcgccaag
     5221 atgacggtag gtttgccgtc ggcatggttc gctgcgcggt cgtaggcgtt gtacactttt
     5281 tgcgggtcgt ggccgccgcg gttcaatgcc cagagttgct catcggtcat atcggcaacc
     5341 aatgctttca gttcgggcgt attgaagaag tgttcgcgca catacgcgcc gtctttggat
     5401 ttgtaagttt ggtagtcgcc gtccaaacat tcttccatac gttggcgcag gataccgtct
     5461 ttgtctttcg ccaagaggcg gtcccagcgg cggccccaaa tgactttgac gacattccag
     5521 ccggcgccgg caaagttgcc ttccaattct tggatgattt tgccgttgcc gcgtaccgga
     5581 ccgtccaagc gttgcagatt gcagttgatg acgaaaatca ggttgtccaa gccttcgcgt
     5641 gcagccagtg cgattgcacc ttgagattcg ggttcgtcca tttcgccgtc gccgcagaaa
     5701 caccatactt tacggccttt ggtttttgcc aaaccacgcg attccaagta tttcaggaaa
     5761 cgcgcctgat aaatcgccat gatgggcccc aagcccatgg atacggtcgg gaactgccaa
     5821 aagtcgggca agaggtgggg gtgcggatag gaaggcagac cgtgtccgtc cacttcttgg
     5881 cggaagttgt tcagctggtc ttcggtcaga cggccctcga cgaatgcgcg tgcatagatg
     5941 cccggggcga cgtgaccttg gaagaagacc aaatcgcctt cttcgccttc gcctttggct
     6001 ttccaaaagt ggttgaaacc gacttcgtac atggtggcgg cagattggaa agatgcgatg
     6061 tgcccaccca gttccaaatc tttcttgccg gcgcgcaata cgatggcggc ggcgttccag
     6121 cgcacgaatg cgcggatgcg gtgttcgatg ttttggtcgc ccggaatgcc tttttcgttt
     6181 tcaaccgaaa cggtattcaa atacggggtg gtcgtgccgt gtggcatacg tacgcccttg
     6241 tcgcggcagt atttgaccag gttttccaag aggtattgcg cgcgttcgcc gccttcatat
     6301 tcgaggacgg agcttaaagc gtccagccac tcttgggttt cgatagggtc aacatcgtgt
     6361 aattgggtgg acatagtatc tatcctttat gttgagtgtt taatcggcag ggagggtgta
     6421 gccaacccct ttattttcgt ttgtgaaaaa taataagcaa aaacgaaaat ttctaaatca
     6481 aaccgaaaaa aataatttaa gtttaatgtt ttcaaaaaat tataaaagtt ttggaaacaa
     6541 gggaaaactt gaaaaaaatg ttcgatctgc tccacacgaa atcctatcag caatcgggtc
     6601 tgctggcaaa taaccatgcc tgtaaaagca ggttttatac tgaagttggc gtttggtaag
     6661 gtcaagtctt tttgatttcg aaatattctg tttgattttg gcgatatttt ttcggaattg
     6721 tggtatatcg aatcattatg tctctgtcgg caattttcct attgtagaaa atctatacac
     6781 aagctttgtg gataaccgtg ttgataagcc acggcttgtg ttaaaaaagt cttacgcaac
     6841 aaatgtacgc ttattttgat taaaaaatag gcacatttaa atctataaaa atcatgtgct
     6901 tatgttcaat atgatgtttc tggtcatgtt tttgccgtct gttatgggat ttgtcccgcg
     6961 cttggggcag ggggtgaatt ttggaggttg gtgcaaaacc tgcttttttc gcgttacaat
     7021 ctgcacccga tttacttttg aatttgcctg tttggaaaaa tacttttatg aaagccagcc
     7081 aattctttat ctctacttta aaagaagccc ctgccgaagc cgcgcttgcc agccacaagc
     7141 tgatgattcg cgccggcctg attaaagcca acgcgtccgg cctttatact tggatgccga
     7201 tggggctgcg cgtgttacgc aaagtcgaaa acgtcgtgcg cgaggaaatg gcgcgcgcgg
     7261 gcagcgtgga gctgctgatg ccggtggtgc agcctgccga gctgtggcag gaatccggcc
     7321 gctgggaatt ttacggtaaa gaactgctgc gcctgaaaga ccgccacgac cgcgatttct
     7381 gcatgggccc gacctgcgag gaagtcatcg ccgacatcgt gcgcaaagaa atcaacagct
     7441 acaaacagct gccgaaaaac ttttaccaca tccaaaccaa attccgcgac gaagtgcgcc
     7501 cgcgtttcgg cgtgatgcgc gcgcgcgagt tcgtcatgaa agacgcttat tccttccatg
     7561 ccgactacgc ctcgcttcag acgacctatg atgccatgta tgacgcttac tgccgtatct
     7621 tcacccgtct gggcttggca ttccgccccg tcgccgcaga caccggcagc atcggcggta
     7681 cgggttcgca cgagtttcaa gtgttggcgg aaagcggcga agatgttatt gcgtacagcg
     7741 acacttccga ttacgccgcc aatatcgagt tggcaccgac cttgccgctt aaaggcgaac
     7801 gcgccgctgc tcaggctgtg ttgaccaaag tacatacacc aaacgtcaaa accattgagt
     7861 ctttggttga attcctgaat attccggttg aacaaaccct caaatccatc gtggtcgaag
     7921 gcgaaaacga aggcgaaatc gtcttattac tgttgcgcgg cgaccatgag ttcaacgaca
     7981 tcaaggcaga aaaactggcg ggcgtgaaat cgccattgac gatggcaagc cctgccgcga
     8041 ttgtcgaaca gttcggcgca aacggcggct ctcttggtcc tgtcggcttc aaaggcaaag
     8101 tctatgccga tttcgctacc gaaaaaggcg cggactgggt catcggtgcg aacgaagacg
     8161 gctaccacta taccggcttc aacttcggtc gcgatgccgc cgagcctgag tttgtcgatt
     8221 tgcgcaacgt cgtcgaaggc gacgaaagcc ccgacggaca aggccgtctg aaactggcgc
     8281 gcggcattga agtcggacac gtcttccagt tgcgcgacaa atatactaaa gccatgaacg
     8341 tcagcttcct cgacaacaac ggcaaatcgc aaattatgga aatgggctgc tacggcatcg
     8401 gcatcacccg cgtcgttgcc gccgccatcg agcagaataa cgacgaaaaa ggcatcatct
     8461 ggaccaaagc aatggcgccg tttgaagtcg ttatcgtgcc gatgaactac aaaaaatcag
     8521 acaccgtacg cgaagccgcc gacaaaatct atgccgaatt gctggcggca ggcgcggatg
     8581 tgctgctgga tgaccgcgac gaacgcgcag gcgtattgct gaacgattcc gagctgttgg
     8641 gtattccgca ccgcatcgtc atcggcgacc gcgctttgaa agaaggcaat gtcgaatacg
     8701 ccgaacgccg caacaacgaa gcgcaggcag ttgcaatcgg agaaattgtt gcgcgtgtaa
     8761 cagcttcatt aaatgggtaa gcttggtcaa atgccgtctg aaaataattt cagacggcat
     8821 ttttatttgg ggagaaagtg aagtggcttc agtattttta gaaggagaag cagtcgaggt
     8881 cggcaatatt ttctgtatcg gcaggaacta tgccgcacat attgaagagt taaaaaacga
     8941 aataccttcc gaacctgtgg tgtttatgaa gccgtcaggc agcattttga atagcggcgg
     9001 tacaatcttg ctgcccgagt ttagtaggga tgtgcagttt gagtgcgaac tcgtgctgct
     9061 ggtcggtaag gattcagacg gcatgggaga gggtgaagat attttgggat gtgtggcggg
     9121 gtacggcgtg ggtttggatt tgaccgcgcg agacatccaa tgccatctga aggcgcaggg
     9181 gctgccctgg ctgaaggcaa aagggttcag gcattccgcg tgcgtgtcgg attttgcggc
     9241 ggcagacaga atcggcaatc ccgagaaggt gttgttttca ttaaaacaaa acggcgtatt
     9301 gaaacagcgc ggggataccg gtttgatgat ttacccgatt cgggaaattt tacacaaact
     9361 ggcggcggat tacggtttgg ggaaagggga tttggtgttt accggaacgc cctccggcgt
     9421 gggcgcaatc ggtgcggggg acaatttggc attggagctg gacggcttgg tgcgcgcctc
     9481 tttcacggta gggtgttaac cgcgccgctt aaccctcacg cccgttgaaa cattccgccg
     9541 gaccgccctc gaaaaaaccg tgcaagatgg cttgcgccgc cacttggtcg agcaccgatt
     9601 tgcgtttttt gccgaagacc tgtgcttccg aaagcaggct ttcggcatag acggacgaca
     9661 gccgttcgtc aacccaatag acggggagat tgaacctgcc gttcagcctg cgtccgaact
     9721 tgcgcgacag gtgcgtcatt tcatgtttcg tgccgtcggt atgcacgggc agtccgacga
     9781 caaaataacg cggctgccat tcttgaacca gcttggcgat tgccgcgaac ttttcatcgt
     9841 tgctgccgcc ggtaacggtg ctcaaaggat gggatagccc taattccgcg tctccttgtg
     9901 ccacgccgat acgcgcttcg ccgaagtcga atgccagtgc cgttcctttt ggaattttat
     9961 gcatggccgg ctcccgaaaa cagggcgagc gggtcgatgc cgagtttggc gaatgcggcg
    10021 gcgtaacggt gttcgtaggg gatgtcgaac aggatgtgtt cgtcggcggg aacagtcagc
    10081 cacgcattgt cggcaagttc gcgttcgagc tggccttttc cccagcttga atagcctatg
    10141 ctgatcaagg ctttgtcaac cgcaccttcg cgtgaaatat tttcaatcac gtctcgggaa
    10201 gaagttagcg cgatattgtc tgaaacgccg atactgcttt gccagttgcc gatcggggta
    10261 tgcacgacat aacctcgctc gacttgcacc ggcccgccca tcatcacgct gtcgtgctgc
    10321 atccgcatgg ggatgttttt gccggtggcg gaaaaaatca tgtccatcgt aatcggagag
    10381 ggtttgttga tggcgatgcc gagtgcgccg tcttcatcgt gtttgcagat atagacgacc
    10441 gattgtgaaa aaaacgcgtc ttccatatcg ggcatggcga cgagaaagtg gttggagagg
    10501 ttcatatcag cgtttgtcgg gattttctat cgggttggaa atcatgcagg tgccggcaaa
    10561 cagtttgccg gaagtatcgg cttgcacccc atcggtttgc ggtatgccgc cgccgaaaca
    10621 ggaaaccgcg cttaaggaat ttttcggcga gccttggatg attttatcgc tgtacaccaa
    10681 ataggcgaag gttttgcgtt tggggtcgta ataacggaca atctgccggc tcttgaacgc
    10741 gaagctcgca ccgtgtttga aaacttcttt cggtttgcgc acggcggttt cgtcaaaaga
    10801 aatcgaagat gccgtctgaa cgcacgaaac cgatgcgtcg gacgcgtcct cttccaaatt
    10861 gaccatttcc ttcaagccgc ctttttttgc atacgaaata taacaggcaa ccccttgaac
    10921 gtcgggatcg tcgaatcctt ccacttcgat acggtcgttt ttgcccagca tgttgaaaac
    10981 ggtacttgcc cgtccgattt tatcggtttc gccgctgccg caggcagtca gcaggacggc
    11041 ggcagacagc agtagcagtc tgttcatcaa aatttcctta tcggttgaat ccccgttttc
    11101 cgtgaaggcg gcagggcttg ctttgtttgg aggggcgcac gttcccaaaa tcgggcaaca
    11161 tacggtatgc ggcttaatgt gctaccctgt gtgtcggaac acaatatccg ttgcggcatt
    11221 gaaaatatga cgcacaatat aaggctgttt gagccgattg caagtatccg cgcaaagaag
    11281 gcgcaagatt gggcaaacgg cattttcaga cggcataact gacagtataa tccaaacgtc
    11341 cggctgcttt cgtgcagcct tcagtattat ccaacttttt ccgaaggtcg aaccatgcgc
    11401 tacaaacccc ttctgcttgc cctgatgctc gttttttcca cgcccgccgt tgccgcccac
    11461 gacgcggcac acaaccgttc cgccgaagtg aaaaaacaga cgaagaacaa aaaagaacag
    11521 cccgaagcgg cggaaggcaa aaaagaaaaa ggcaaaaatg gcgcagtgaa agataaaaaa
    11581 acaggcggca aagaggcggc aaaagagggc aaagagtcca aaaaaaccgc caaaaaccgc
    11641 aaagaagcag agaaggaggc gacatccagg cagtctgcgc gcaaaggacg cgaaggggat
    11701 aagaaatcga aggcggaaca caaaaaggca catggcaagt ccgtgtccgg atccaaagaa
    11761 aaaaacgcaa aaacacagcc tgaaaacaaa caaggcaaaa aagaggcaaa aggacagggc
    11821 aatccgcgca agggcggcaa ggcggaaaaa gacactgttt ctgcaaataa aaaagtccgt
    11881 tccgacaaga acggcaaagc agtgaaacag gacaaaaaat acagggaaga gaaaaatgcc
    11941 aaaaccgatt ccgacgaatt gaaagccgcc gttgccgctg ccaccaatga tgtcgaaaac
    12001 aaaaaagccc tgctcaaaca aagcgaagga atgctgcttc atgtcagcaa ttccctcaaa
    12061 cagcttcagg aagagcgtat ccgccaagag cgtatccgcc aagagcgtat ccgtcaggcg
    12121 cgcggcaacc ttgcttccgt caaccgcaaa cagcgcgagg cttgggacaa gttccaaaaa
    12181 ctcaataccg agctgaaccg tttgaaaacg gaagtcgccg ctacgaaagc gcagatttcc
    12241 cgtttcgtat cggggaacta taaaaacagc cagccgaatg cggttgccct gttcctgaaa
    12301 aacgccgaac cgggtcagaa aaaccgcttt ttgcgttata cgcgttatgt aaacgcctcc
    12361 aatcgggaag ttgtcaagga tttggaaaaa cagcagaagg ctttggcggt acaagagcag
    12421 aaaatcaaca atgagcttgc ccgtttgaag aaaattcagg caaacgtgca atctctgctg
    12481 aaaaaacagg gtgtaaccga tgcggcggaa cagacggaaa gccgcagaca gaatgccaaa
    12541 atcgccaaag atgcccgaaa actgctggaa cagaaaggga acgagcagca gctgaacaag
    12601 ctcttgagca atttggagaa gaaaaaggcc gaacaccgca ttcaggatgc ggaagcaaaa
    12661 agaaaattgg ctgaagccag actggcggca gccgaaaaag ccagaaaaga agcggcgcag
    12721 cagaaggctg aagcacgacg tgcggaaatg tccaacctga ccgccgaaga caggaacatc
    12781 caagcgcctt cggttatggg tatcggcagt gccgacggtt tcagccgcat gcaaggacgt
    12841 ttgaaaaaac cggttgacgg tgtgccgacc ggacttttcg ggcagaaccg gagcggcggc
    12901 gatgtttgga aaggcgtgtt ctattccact gcaccggcaa cggttgaaag cattgcgccg
    12961 ggaacggtaa gctatgcgga cgagttggac ggctacggca aagtggtcgt ggtcgatcac
    13021 ggcgagaact acatcagcat ctatgccggt ttgagcgaaa tttccgtcgg caagggttat
    13081 atggtcgcgg caggaagcaa aatcggctcg agcgggtcgc tgccggacgg ggaagagggg
    13141 ctttacctgc aaatacgtta tcaaggtcag gtattgaacc cttcgagctg gatacgttga
    13201 tcggattgcc gaaacggtag gctttatgca aatcgtgccg atatggtgcg ctgttccgtg
    13261 aagtttgccg tttgccgtac ctgtattttc cggtttttgg aaatcaagag agaaagttaa
    13321 agaagaatgt cgaaacctgt ttttaagaaa atcgcacttt atactttggg tgcaatcagc
    13381 ggcgtggccg tcagtctggc ggtgcagggt tttgccgccg agaaggacag gcgggataac
    13441 gaagtcctgc cggtgcaatc catccgcaca atggcggagg tttacggtca aatcaaggca
    13501 aactactatc aggacaaacc cgatgccgat ttgtttgaag gtgcgatgaa gggtatggtg
    13561 gccggtttgg atccgcattc cgaatatatg gataaaaaag gttatgccga gataaaggag
    13621 tccaccagcg gcgaatttgg cggcttgggg atggaaatcg ggcaggaaga cggatttgtc
    13681 aaagtggttt cgccgattga ggacacgcct gcggaacggg cgggggtgaa aagcggcgat
    13741 ttcattgtga aaatcgataa tgtttcgaca cgcggcatga cggtcagcga agcggtgaag
    13801 aaaatgcggg gcaagccggg tacgaagatt actttgacgc tgtcgcgcaa aaatgccgac
    13861 aagccgatag tcgtcaacct gacccgtgcc attattaaag tgaaaagcgt ccgccatcac
    13921 ctgatcgaac ccgattacgg ctatatccgc gtgtcgcagt tccaagagcg gacggtcgaa
    13981 agcgtcaata ccgccgcaaa agagctggta aaggaaaata aaggaaaacc gctcaagggg
    14041 ctggtgttgg atttgcgcga cgaccccggc gggcttttga ctggcgcggt cggcgtgtcg
    14101 gcggcatttc tgccgtctga agcagtcgtc gtcagcacca agggacgcga cggcaaagac
    14161 cgcatggtac tgaaagccat tcctgaagat tatgtgtacg ggatgggcgg cgattcgttg
    14221 gcgggcattc ctgccgagtt gaaaaccata ccgatgacgg tattggtcaa ttccggttcg
    14281 gcttccgcgt cggagattgt cgcaggtgca ttgcaggatc ataaacgcgc ggtcatcgtc
    14341 ggtacgcaga gcttcggcaa aggttcggtt cagactttga ttcctttgtc caacggcagc
    14401 gcggtcaagc tgacaacggc actgtattat acgccgaacg accgttctat tcaggcgcag
    14461 gggattgttc ccgatgtcga agtaaaagat aaggaacgca tttttgaaag ccgcgaggcg
    14521 gatttggtcg gacacatcgg caatcccttg ggcggcgagg atgtgaacgg tgaaaccctt
    14581 gccgtgccgc ttgaaaaaga tgcggataag cccgctgtaa aagaaaaagg taaaaagaaa
    14641 aaggacgagg atttgtcttc aaggcggatt cccaaccctg ccaaagacga ccagttgcgg
    14701 aaagctttgg atttagtcaa gtcgcccgag cagtggcaga agtctttggg gctggcggcg
    14761 aaaaagccgg tttcaaataa agataagaaa gataaaaaag ataagaagta ggttttgacg
    14821 atgccgtctg aaaggatttc agacggcata ttatgtttga aaggacaggt tatgagcgat
    14881 tttttaccgg aatttgaacc gtgcgg
//
LOCUS       DAXHRI010000035        14456 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXHRI010000035 DAXHRI010000000
VERSION     DAXHRI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.035"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(117..461)
                     /locus_tag="ACFM75_000808"
     CDS             complement(117..461)
                     /locus_tag="ACFM75_000808"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC6255388.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDGAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(454..1452)
                     /locus_tag="ACFM75_000809"
     CDS             complement(454..1452)
                     /locus_tag="ACFM75_000809"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6255389.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(1483..3756)
                     /locus_tag="ACFM75_000810"
     CDS             complement(1483..3756)
                     /locus_tag="ACFM75_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC6255390.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPVRTMPGHRA
                     LAVLRGRNEGVLNIALKYRPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYKNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGTEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(3820..4470)
                     /locus_tag="ACFM75_000811"
     CDS             complement(3820..4470)
                     /locus_tag="ACFM75_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6255391.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(4467..5264)
                     /locus_tag="ACFM75_000812"
     CDS             complement(4467..5264)
                     /locus_tag="ACFM75_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6255392.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5264..6427)
                     /gene="ctrB"
                     /locus_tag="ACFM75_000813"
     CDS             complement(5264..6427)
                     /gene="ctrB"
                     /locus_tag="ACFM75_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC6255393.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDVFSRFNGFGFRGEEEAFYQYYKDKLNINFDAISGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(6442..7617)
                     /gene="ctrA"
                     /locus_tag="ACFM75_000814"
     CDS             complement(6442..7617)
                     /gene="ctrA"
                     /locus_tag="ACFM75_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC6255394.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            7744..8877
                     /gene="neuC"
                     /locus_tag="ACFM75_000815"
     CDS             7744..8877
                     /gene="neuC"
                     /locus_tag="ACFM75_000815"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFC6255395.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITLISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSINVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            8881..9567
                     /gene="neuA"
                     /locus_tag="ACFM75_000816"
     CDS             8881..9567
                     /gene="neuA"
                     /locus_tag="ACFM75_000816"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HFC6255396.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGELIAEEAKNFGVEIVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            9568..10617
                     /gene="siaC"
                     /locus_tag="ACFM75_000817"
     CDS             9568..10617
                     /gene="siaC"
                     /locus_tag="ACFM75_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HFC6255397.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            10701..12188
                     /locus_tag="ACFM75_000818"
     CDS             10701..12188
                     /locus_tag="ACFM75_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HFC6255398.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQKHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            complement(12684..13637)
                     /locus_tag="ACFM75_000819"
     CDS             complement(12684..13637)
                     /locus_tag="ACFM75_000819"
                     /inference="COORDINATES: protein motif:HMM:NF042780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HFC6255399.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGIVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            14020..>14456
                     /locus_tag="ACFM75_000820"
     CDS             14020..>14456
                     /locus_tag="ACFM75_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6255400.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDT"
BASE COUNT         4283 a         3271 c         2813 g         4089 t
ORIGIN      
        1 gtatcccccg gttgcatatc ttccgtatag ggcattttac gtatcatggc gcgacgcatt
       61 aaagtaaacg ccaaagcgcg tttatactgg ttatccagtt tccctatatt tttacgttac
      121 aaatcaagtt gttggtattc atcgtgaaaa tagaattttt cggcataacg ctgactcata
      181 aaaccggcat gcgggctacc tgaaaaaatc attgcgccat catcgtcatt taatgtttca
      241 tggttgtttt ctattaatgc taaagcaatt tggtaattaa ttgccaaaat atcgttagta
      301 atcacacgat agccgcgttt tttggcttca taggcaaagg aacagcctcc actaaataca
      361 tctgcaactg tatctacatc agacggaagc tggtcacaaa tccaggaagc tattttctct
      421 ttattaccga tatagttaat tttcggatat tgcttattca ttttcactca tttttaaaat
      481 actttcagca atagcttttg ccaataaagg cggtacggcg ttaccaacct gctgctgggc
      541 aattttgctg ccgcaaaaaa taaaattatc agggaaagat tgtaaggcag ctaattcacg
      601 aacggttaaa gcccgattct gttcatagtg aaaaactttg cgcatatctc ctgtaataca
      661 aacggctggt ttgttgctgt tgtaacggat gtatttacgg atatcacctg ttttcggacg
      721 taatggttca ggaatatcgt tacggttacc tccattttta acaaatgcca ttttttctaa
      781 catttgtgcc gaatgattca tagcttcatg atttgcaacg tgtggattgc tttcgccagc
      841 agccagtttt ggaaaatgtc ctattgctga tccaacagtc tgatgggaaa tctgcaaagg
      901 ttcgggaaag gaaattttgc ctttatccct cctcccgata aatatcactc ggctacgtat
      961 ctgaggaaca ccgaaatcgg ctgcactcag tatcttacat tccaccgaat aaccgatatt
     1021 ctgaaatgct tgaataatct caatacgtgt tttacctgaa ttgtgtgtat agagtcgcgc
     1081 tacattttcc ataacaaaaa aatatggttg gacaatttta actattcgga caaactcttt
     1141 aaataaatgg ttgcgtgggt catctgtaaa tgtccgtcca atctttcctg ccatactaaa
     1201 accttgacaa ggtggtcctc caataatcaa atcaactgct tgtccgttaa gacaattgat
     1261 taaatcttgt tcggttagtg tggttaaatc tttttgcagt aattgatgat gggggaagtt
     1321 ggtacggtaa gtctgacaat aatcagactc catttcaaca gaaagcaatt gttggaatcc
     1381 ggcttgttca aaacccaagg ataggcctcc tgctcctgag aaaaggtcaa tataggtaag
     1441 tggtgtgtgc ataagtcaaa atccataaac tcttcgatta ttttaccgct tcagcttcgc
     1501 aaacgcatcc gccatcgccg aattggctgg ggcgcggtcg ttgcgttggg gtttgcggcc
     1561 ggctgtccgt tcgcggctgc ggttttcaga cggcatttta tgttttgcgc cgcccggttc
     1621 gtcatccaag cgcatggtca gcgcgatgcg tttgcgtgca gcatcgactt ccagcacttt
     1681 cactttcacc acgtcgccag ctttcaccac ttcgcgcggg tcttggacga acttgttgga
     1741 cagggcggag atgtgcacca agccgtcctg atggacgccg atgtccacga acgcgccgaa
     1801 gttggcgacg ttggaaacca cgccttcgag tatcataccg acttgcaagt cgctgatttc
     1861 gtggatacct tcggcaaacg atgccgtctg aaactcgccg cgcggatcac ggccgggttt
     1921 ttccagttcg gacaggatgt ccaaaatggt cggcaggccg aagcgttcgt cggtgaagtc
     1981 ggacgctttg atttgcttca cgcgctcgcg gttgccgatg agttcggcgg cgctaatgcc
     2041 ttgttgcgcc agcattttgg cgacgacggg ataggcttcg gggtggacgg cgctcgcgtc
     2101 cagcggctct gtgccgccgt taatccgcaa aaagcctgcc gcctgctcga aggttttttc
     2161 gcccaaacgc ggtactttca gcaatttttt gcggctgtcg aacgcgccgt tttcatcgcg
     2221 gtaggcaacg atgttttggg caagggtttg attcaaaccg gaaattcgcg ccaagagcgg
     2281 ggcggaggcg gtgttcacgt ccacgccgac ggcgttcacg cagtcttcga ccaccgcgtc
     2341 cagcgatttg gcgagctggc tttggttcac gtcgtgctga tactgcccca cgccgatgga
     2401 tttggggtcg attttgacca actcggcaag cgggtcttgt agcctgcggg cgatggacac
     2461 cgcgccgcgc agggaaacgt ccaagtcggg gaactcgcgc gccgccagtt cggacgcgga
     2521 ataaatcgac gcgccggctt cggacacgac gattttatgc agccccattt tcgacatttc
     2581 acgcaccagt tcgcccgcga ttttgtcggt ttcacggctg gcggtgccgt taccgatggc
     2641 gatgagcttc acgccgtgct gtttaatcag gcgcgacagc gttgccaaca tattgttttc
     2701 ttgatgtaaa tagacaatga cggtatccag cagcttgccc gtgtcgtcca ccacggcaca
     2761 tttcacgccg ttcttgtatc cagggtctag acccaaagtg gtcagccgtc cggcgggtgc
     2821 ggcgagcagc aagtctttga gatttcgggc aaacacggta atcgcgtcgg tgtcggcggc
     2881 ttctttcaaa cgattgagag cttcgagttc caacgacaaa aagattttcg cgcgccaagt
     2941 caggcgcacg gtgtcgcgca gccatttgtg gccgtctgaa accttgaaac ggcgggcgat
     3001 gatttgctcg tattcgcttt gctgcgtaat cggcgtgtcg tcgggccggt atttgagcgc
     3061 gatgttcaac acgccttcgt tgcggccgcg caaaaccgcc aacgcgcggt ggccgggcat
     3121 agtgcggacg ggttcgcggt ggtcgaaata atcgctgaat ttttcgcctt cggtttcttt
     3181 gcctccaaca acttgcgcgt ggatttcggc ttcgttccac agcttgtcgc gcagcgtgcc
     3241 gatgagttcc gcgtcttcgg caaactgctc catcagaatc gcacgcgcgc cgtccaacgc
     3301 ggctttggca tcggggacgt tttcgttcag gtagccttgt gcggcggctt ccacgtcctg
     3361 cgactgctcg gcaagcaaca cgtccgccag cggctgcaaa ccgtgttcgc gcgcgatttg
     3421 cgctttggtg cggcgtttgg gtttgtaggg cagatacagg tcttccagcg cggttttgtt
     3481 atcggcggct tcgatttgcg ccctgaggtc gtctgaaagc ttgccttgct cttcaatgct
     3541 ttttaaaaca acggctttgc gctcttccaa ctcgcgcaga tattgcagcc gctcggcaag
     3601 ccggcgcaac tgcgtatcgt ccaacccgcc cgtcgcttcc ttgcggtagc gggcgataaa
     3661 cggcacggtc gcgccgtcgt ccaaaagctc gacggcggcg gtgatttgcg cggcagtcgc
     3721 ggagagttct tgggaaagaa tttgagtaat gttcatcaat agaattccaa cggacaggcc
     3781 gtctgaaatt tcagacggcc tgatttaaaa acaatcgctt taaggcagcg aattataata
     3841 ttcgtaggct ttgtccatat cttcaaactg gtacatatgc cctttttcca gcaccatcgc
     3901 attatcgcaa tattgcttca tggcgctgtg gctgtgcgac accaagatga tggaacggtc
     3961 tttgcgcttt tcaaacaact cgtacttaca tttatcggca aaacgcgagt caccaactgc
     4021 aatcacttcg tcaatcaaat agcaatcaaa ctctaccgcc aacgacaaag caaatgccaa
     4081 acgcgccttc atacctgaag aataacgctt gaccggctcg taaagatatt gccccaactc
     4141 ggaaaactct tcggtaaagt tcttcacata atcgatatcg acattgtaaa tccggcagat
     4201 gaaacgcaaa ttgtccatac cggtcagact gccttgaaac gcaccggaga atgccaaagg
     4261 ccaagaaata ctcattgtcc gcttgatttc acccgtggtc ggcggctcaa cgccactgat
     4321 caaacggatg agcgtcgatt tacctgcacc gttgcggccg agaataccga ttttctcgcc
     4381 cttctccatt ttgaagctaa tatcgtgcaa gactgtccgc caaccttggc gggtcagata
     4441 gcgtttggaa acgtgttcaa ctgaaatcat tgcggctcga ctcctttact gaatttactg
     4501 accatcgcca agccaaacaa caacaacacc agattgcaca atacgatata ccaaggattt
     4561 tcataggtaa ttacatcgct gccaaaatat ccggcacgga acatttctgt gccatgcacc
     4621 atcgggatca tcaatgcata ctcttgcact ttcggaggca aattatgtac aaaaaagaat
     4681 gcacccgata acggcatcat cacaaaagtc aatgtgcccc aaatcttgcc aaacggctcg
     4741 aaattaaagg caatcgaaca aatcaccaaa cccaaaccaa tcgcaaaaaa agccatcaaa
     4801 agccaagcca tcagcatata aaacatatct gccggcattt caatccagcc aatcgcaatc
     4861 aataccgcca taatcacaat ctgcgcaatg gttgcaccag caatttccaa aatcatgcgc
     4921 gccaagatgg tatccaaaac tcttacattg cggtgataaa gcaagctggc atttgaagaa
     4981 atcgacccaa ctgcccgttt tgaggcatta cgccacatca tcaacatcgg atagccagta
     5041 atcgcaaatg cgacaatatt caaagttgaa tatcggtctg cctttaaaaa tttccacatc
     5101 aagacgataa cgaatgtcat cagcaacggc tcaacaaaca gccataaaaa gccaatatta
     5161 ttgcgaccgt aacgggtgat aatttcccgc atcaaaagcg caccgattac gcgcctttga
     5221 atggctaaag attcccaaaa tgatgtttta tgcaaggctt tcatcagttt ttatgctcac
     5281 gaatgctggc agtcaacagg ctcaaaatac cataaaccat caagccgata atcagagtgg
     5341 caacaatgtt gtataaccgt ttaggctcat gtgccaaatc cggcaggctc ggttgcgaga
     5401 tcacttccaa ataaagctgc tgacggtctg cttcaacctt ggcactttcc aaagaagtca
     5461 tggcggctgc caactgctgc tctgccaact ggttttccaa atacacacgc tgatattcgg
     5521 cagcctgatt agacaacgaa gaatgcccac cgcccgaaat ggcacgtaac tgttggtcaa
     5581 tttctttacg caagctctgc tcacgcgcct gcaaacccgg aatctgcgga ttctccggag
     5641 tgactgcttt cacctgatcc agctgggttt gaatcacaat caattcatct tgcagcttgg
     5701 aaaccaaccc catttgcact tccgattgcg ctttcaaatc aaaaacgcca ttggcaatcc
     5761 ggtaatccgt cagattctga gaggcttcct ttacccgctc tgccgccgtt ttcactactt
     5821 cttccgcata gcgcaccgta tcagcacgtg cacgatcgtt caactggtta atcaatgctt
     5881 caccttgttt taacaaagct tcattgattt tcttagattc aagcgcatca aatgatgtaa
     5941 cgttaagcgt agagatacct gaaatcgcat caaaattaat gttcagctta tctttatagt
     6001 attgataaaa agcctcttcc tcaccacgga atccaaaacc attaaatcgg ctaaatacat
     6061 cgcccttagt ttcataaaat tcacgcaccg gcaagatttt acgcagttca tccaaagacg
     6121 agcgcgaacg catatactcc ccaaccgtgt aaatatcatc ttgcgcacgg gcaaaacctg
     6181 tgccctgcaa aatggcaccc aggccattga gagaagattg gcttttaggc gagcgcacca
     6241 caaagctcga ttgcgacgta aaacgatcgg aagcgaagaa gccgaaatac accaacgaaa
     6301 ttaccgtagg gataatcacc gttacccaaa ataaagggct tagcttttta atccaacttt
     6361 tctttttcgg ctttttacgc tcggctttgg tttcagtggc aactgccaca ggaagttgct
     6421 cagacatctt aattactcac attaattagt taaattatta atactgttcg cgccactggt
     6481 aaccggcgag aacacaaacg acaagaattt ctgcacttca gccaacggcg cattcgacac
     6541 atacaataca tctttattct tcacaggaaa gcgctgcata gaaaatagcg aatgcgcatc
     6601 agccatattc acacgatata ccgttggaat ctctgcctca ctgccataac cttgagcaat
     6661 ccatttatcc tgacgttctg ccggcaattc caccaatggc gtatagcgga acacaaacac
     6721 accacgcgca tcagaacggc gatcttgcaa accgcccata cggccaatgg cttcagaaag
     6781 cgataagcct ctggctgaaa aaccgatttc ttgtgttctc cccaccgcac ccatagacgt
     6841 aaaggtatag ggattggtaa tcatggtaac cacatcaccg cgacgcagca aaatattttg
     6901 tcgcggattt gcaactaaat cttccaaggc aacagttcgt actacattgc cacgtgtcag
     6961 ctgcacattc gtatcctgca catttgccgt tgaaccacct accgcagcca ccgcatccaa
     7021 cacacgctca ccggctgccg tcagcggcat acgcacacta ttcccagcac gaatcaccga
     7081 cacattcgcc gcattattct gcaccaaacg caccatcact tgtggctgat tggccatttt
     7141 tttcaggcgg cctttaataa tttcctgaac ctgaccaggc gttttaccga ccaccgaaat
     7201 atcgccaaca aacggcacag aaaccgtacc acgtgccgtg accaactgct ctggcaactt
     7261 agtttgatgc gcactacccg agcccatcga agaaaggcca ccaccaaaca atactgccgg
     7321 cggcgcttcc caaatcataa tatccaatac atcaccaata tttagcgtac cagccgaagc
     7381 ataaccatcg ccaaactgag tgaatgactg atttatctga gccttatata ataactgagc
     7441 aaccgtatga ttcacatcaa tcagctccac ttcaggaatt tgaacttcag attgttgccc
     7501 taaagagaca attttttttg cgctggggcc tgatgaagga atcgcagagc atcctacaat
     7561 taaacttcca cacaataata atactgcgtg acgaatataa aatttcactt taaacacaag
     7621 ccaaatccca atatacttat aaatggccta attatagcac ttaatcgaaa taaatttatg
     7681 agtacgtaga gtataattag tattcttctt tccaacttcc ttatacttat agattctaaa
     7741 atcatgaaaa gaattctttg cattacaggt accagagccg acttcggcaa gctaaaacct
     7801 ttattagcct atattgaaaa tcacccagac cttgaattgc atttgattgt aactggtatg
     7861 catatgatga aaacatatgg cagaacctac aaggaagtaa ctcgagaaaa ctatcaacat
     7921 acatatctgt tttcaaatca aatccaaggt gaaccaatgg gtgccgtttt aggcaatacc
     7981 attacgttaa tctctcgtct atctgatgaa attgaacctg atatggtcat gattcacggc
     8041 gaccgtttag aagcactagc aggcgcaact gtaggtgcat taagcagccg tttagtttgc
     8101 catatcgaag gtggtgaact atctggtaca gtagatgact ccattcgtca ttctattagt
     8161 aaactttctc atatccactt ggtagcaaat gaacaagctg tcactcgcct agtgcaaatg
     8221 ggagaaaaaa gaaagcatat tcacatcatc ggctcccccg atttagatgt tatggcctct
     8281 tccaccctcc catccttaga agaagtcaaa gaatattacg gtttaccata cgaaaattat
     8341 ggtatttcta tgtttcaccc cgtgactaca gaagcacatt taatgccaca atatgcggcc
     8401 caatatttca aagcattaga attaagtggc caaaatatca ttagcatcta ccctaataat
     8461 gacactggca ctgaaagtat tctgcaagaa ttattaaaat atcaatctga taaatttatt
     8521 gcatttcctt ccattcgatt tgaatatttt ttagtcttat tgaaacatgc taaatttatg
     8581 gtcggcaact caagtgcagg tattagagaa gctcctctct acggtgtccc ttcaattaat
     8641 gttggtacac gccaaagtaa ccgccatatg ggaaaatcta ttattcatac agattatgaa
     8701 actaaaaata tctttgatgc gattcaacaa gcatgcagtt taggcaaatt tgaagcagat
     8761 gataccttta atggcggaga tactcgcacc agcacagaaa gatttgctga agtaatcaac
     8821 aatcctgaaa cgtggaatgt ttctgctcaa aaacgtttta tcgatttgaa tctttaaatt
     8881 atggaaaaac aaaatattgc ggttatactt gcgcgccaaa actccaaagg attgccatta
     8941 aaaaatctcc ggaaaatgaa tggcatatca ttacttggtc atacaattaa tgctgctata
     9001 tcatcaaagt gttttgaccg cataattgtt tcgactgatg gtgagttaat tgcagaagaa
     9061 gctaaaaatt tcggcgtcga aatcgtccta cgccctgcag agctggcctc cgatacagcc
     9121 agctctattt caggtgtaat acatgcttta gaaacaattg gcagtaattc cggcacagta
     9181 accctattac aaccaaccag tccattacgc acaggggctc atattcgtga agctttttct
     9241 ctatttgatg agaaaataaa aggatccgtt gtctctgcat gcccaatgga gcatcatcca
     9301 ctaaaaaccc tgcttcaaat caataatggc gaatatgccc ccatgcgcca tctaagcgat
     9361 ttggagcagc ctcgccaaca attacctcag gcatttaggc ctaatggtgc aatttacatt
     9421 aatgatactg cttcactaat tgcaaataat tgttttttta tcgccccaac caaactttat
     9481 attatgtctc atcaagactc tatcgatatt gatactgagc ttgatttaca acaggcagaa
     9541 aacattctta atcacaagga aagctaaatg caaaacaaca acgaatttaa aattggtaat
     9601 cgttcagtag gttacaacca cgaaccattg attatctgtg aaatcggcat caatcatgaa
     9661 ggctctttaa aaacagcttt tgaaatggtt gatgctgcct ataatgcagg cgctgaagtt
     9721 gttaaacatc aaacacacat cgttgaagac gaaatgtctg atgaggccaa acaagtcatt
     9781 ccaggcaatg cagatgtctc tatttatgaa attatggaac gttgcgccct gaatgaagaa
     9841 gatgagatta aattaaaaga atacgtagag agtaagggta tgatttttat cagtactcct
     9901 ttctctcgtg cagctgcttt acgattacaa cgtatggata ttccagcata taaaatcggc
     9961 tctggcgaat gtaataacta cccattaatt aaactggtgg cctcttttgg taagcctatt
    10021 attctctcta ccggcatgaa ttctattgaa agcatcaaaa agtcggtaga aattattcga
    10081 gaagcagggg taccttatgc tttgcttcac tgtaccaaca tctacccaac cccttacgaa
    10141 gatgttcgat tgggtggtat gaacgattta tctgaagcct ttccagacgc aatcattggc
    10201 ctgtctgacc ataccttaga taactatgct tgcttaggag cagtagcttt aggcggttcg
    10261 attttagagc gtcactttac tgaccgcatg gatcgcccag gtccggatat tgtatgctct
    10321 atgaatccgg atacttttaa agagctcaag caaggcgctc atgctttaaa attggcacgc
    10381 ggcggcaaaa aagacacgat tatcgcggga gaaaagccaa ctaaagattt cgcctttgca
    10441 tctgtcgtag cagataaaga cattaaaaaa ggagaactgt tgtccggaga taacctatgg
    10501 gttaaacgcc caggcaatgg agacttcagc gtcaacgaat atgaaacatt atttggtaag
    10561 gtcgctgctt gcaatattcg caaaggtgct caaatcaaaa aaactgatat tgaataatgc
    10621 ttattaactt agttacttta ttaacagagg attggctatt acatatagct aattctcatt
    10681 aatttttaag agatacaata atgctaaaga aaataaaaaa agctcttttt cagcctaaaa
    10741 agttttttca agattcaatg tggttgacaa catctccatt ttatcttacc cccccacgta
    10801 acaatttatt tgtcatatct aatttaggtc agcttaacca agtccaaagc ctaattaaaa
    10861 tacaaaaatt aaccaataat ttactagtaa ttttatatac ttctaaaaac ttaaaaatgc
    10921 ctaagttagt tcatcaatca gctaacaaga atctatttga atctatttat ctatttgagc
    10981 ttcctagaag ccctaataat ataactccta aaaaattact ttatatttat agaagttaca
    11041 aaaaaatcct taatattata cagcctgctc atctctatat gctgtctttt acaggccact
    11101 actcctatct gattagtatt gcaaaaaaaa agaatattac gactcattta attgatgaag
    11161 ggactggaac atatgctcct ttattagaat cattttcata tcatccaaca aaattagaac
    11221 gttatttgat tggaaataat cttaatatta aaggatatat agatcatttt gacatattgc
    11281 atgtcccctt tcctgaatat gctaaaaaaa tatttaatgc aaaaaaatat aaccggtttt
    11341 ttgcgcatgc tggaggaata agcattaata ataacattgc aaacttacag aaaaaatatc
    11401 aaatatctaa aaatgactat atttttgtta gtcaacgcta ccccatttca gatgatttgt
    11461 attataagag tatagtagaa atcttaaaca gcataagttt acaaattaaa ggaaagatat
    11521 ttattaaact acacccaaaa gagatgggca acaactatgt aatgtcttta tttctaaata
    11581 tggtagaaat aaaccctcgg ctggtagtta ttaatgaacc tccttttcta attgagcccc
    11641 taatatactt aacaaatcct aaaggaatta taggcctggc ctctagttct ttaatttata
    11701 caccattact ctcaccctca acccaatgtc tttctattgg agagttaatt attaacttaa
    11761 ttcaaaaata ttcaatggtg gaaaacactg aaatgatcca aaaacactta gagattatta
    11821 agaaatttaa ttttattaat atactaaatg atttaaatgg ggtaataagt aaccctctct
    11881 ttaaaacaga agaaacattt gaaacacttc ttaaatctgc agaattcgca tataaatcta
    11941 aaaactactt tcaggctatt ttttactggc aacttgccag caaaaacaat attaccttat
    12001 tagggcataa agcattatgg tactacaatg cactttataa tgtaaaacaa atttataaga
    12061 tggaatattc agatattttt tatatcgata atatctccgt agactttcat agtaaagata
    12121 aattgacatg ggaaaaaatt aaacattatt actattccgc cgacaataga attggtagag
    12181 atagataaaa atactatgac ttctgatctc catacgtatg cttgttttca aaaagcagca
    12241 ttaagataga taagtgaagc ttgatgagag ccatttttgt ggtcatcgcc aaggtacacg
    12301 cggagagtaa tcgacaacag agctgctgta ttctggcttt taaagcccag tagtaaagtt
    12361 tataaggctg catttctcaa tgtaaaacgc gatattttca cccattatta gggataagta
    12421 gccagtggtt tacactggct actttcatag ttatgaagta ctgggtctga gtgaattcaa
    12481 ttatttccgt ataaaacaca gtagacattt tactaatcgg caaaattata ttagaagaat
    12541 tgagaactac tgaagtcagg taagatacca ttttgcacaa atttaacggc atccctaaaa
    12601 agcattttgg gtggtattta aaggagtaca aaatggtttt aactacagta tgcttagaaa
    12661 tctatagcct tacgcgcttt atattaaatc acctttctca accatttaga atcataaaaa
    12721 aactttttag gatccctaat taacttttta aattttctta aaaaaatagt tttagttgat
    12781 tctatactat tcactgcaga taaatattct tggtaatcat gttgcaatgg cacaacctgc
    12841 tttaatagct tattaaactc atcagttgta atcacctttt tagtagcata gcaatcaagc
    12901 caatccggat ctgctatttt tggggaagtg aaatctattt gatcaaaata aactaaaacc
    12961 ccactaccta aaaaatgctg atttgcatag gatttagaaa agcctaagtc tgcaacaata
    13021 ccaacaggga tacccttaca ttcagcttca aaaagcatag tagatgaata agataaacaa
    13081 taccccattt cttggaaggc ttgcgcggta ctttttcgct ctattgtcaa attactcggc
    13141 aactgaaact gctttgccag ctctatatac gaatgtttat cctgatgcac agtaatatct
    13201 ttatctgcaa cccttagcaa aatagtaaat tctttctcag ggtatttttc agccaaggca
    13261 atcaattttt ttaatgtata aattctttct tcttttttaa atgggatttt aacttggtca
    13321 ataaagtaaa ttttctctcc atgacgacct tttgatgcgt ggcgaaaaat tggataacca
    13381 taaagtatat tttgacaact taatttatat tgtttcttat attcctctgc aattctaaaa
    13441 tcatgcttat tattatataa aacaatatca gcccccatac gagatagaat acttgcctga
    13501 tcaccgaata ctacacctgg aaacaaggta ataatcaatg gccttgaagc aatatttaat
    13561 tgcgcattct gcttaaagaa cctttttaac aacccatttc caaccgataa aattactgcg
    13621 tcataatatt gatgcatatt cttaataaag taatcatcaa tatgaaaaaa aaaattgctt
    13681 ggtctttatc tatccctgat tctaataact gtcgatttga aagaatgttt tctcgacttt
    13741 tgtggatata aatatcaatt tgagcatctt ttatctcttt tgcaacagca tagcccgagt
    13801 taaggaacga gtcataactg gcaattagta atactttttt cactattttc ccttaattct
    13861 acaccatatg aataaaatta tagtatttta ccttagatat caaatatact taaccaaaaa
    13921 aatctgaaga aatctactgc tacttcaggg ataattattt cattaagatt ttacaaccat
    13981 tattatcaat ctatcaaagt atttttagta ttttattcta tgaaaaaaat tctcgttacc
    14041 ggcggcaccg gttttatcgg ctcgcatacc gttgtttctt tgctgaaaag cggccatcaa
    14101 gtcgtgattt tggataacct atgcaattcc agcatcaata tcctgccacg cttgaaaacg
    14161 attaccggcc aagagattcc gttttatcaa ggcgatatcc gcgatcgtga gattttgcgc
    14221 cgtatttttg cggaaaaccg cattgattcg gtgattcatt ttgccggctt gaaagcggtg
    14281 ggtgaaagtg tggccgagcc gatgaaatat tatgataata atgtttccgg cagcttggtg
    14341 ttggcggaag aaatggcgcg tgcgggcgtg tttagcattg tgttcagttc ttcggcgacg
    14401 gtttatggcg atccgggcaa agtgccttat accgaggata tgccactggg cgatac
//
LOCUS       DAXHRI010000036        13915 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXHRI010000036 DAXHRI010000000
VERSION     DAXHRI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13915
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.036"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(122..1447)
                     /locus_tag="ACFM75_000821"
     CDS             complement(122..1447)
                     /locus_tag="ACFM75_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC6255401.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSIINGPDRPV
                     GIPAPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITAHTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1659..2498
                     /gene="efeU"
                     /locus_tag="ACFM75_000822"
     CDS             1659..2498
                     /gene="efeU"
                     /locus_tag="ACFM75_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC6255402.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIAVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2495..3661
                     /gene="efeO"
                     /locus_tag="ACFM75_000823"
     CDS             2495..3661
                     /gene="efeO"
                     /locus_tag="ACFM75_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC6255403.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGA
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3925..5190
                     /gene="efeB"
                     /locus_tag="ACFM75_000824"
     CDS             3925..5190
                     /gene="efeB"
                     /locus_tag="ACFM75_000824"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC6255404.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5260..5589)
                     /locus_tag="ACFM75_000825"
     CDS             complement(5260..5589)
                     /locus_tag="ACFM75_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC6255405.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESASDLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIACKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5665..7035)
                     /gene="glmU"
                     /locus_tag="ACFM75_000826"
     CDS             complement(5665..7035)
                     /gene="glmU"
                     /locus_tag="ACFM75_000826"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC6255406.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDSSGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLEAWLNSLSSNNAQGEYYLTDLIAKAVADGIKIHPVQVRS
                     SHLAAGVNNKLQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGKIELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNQIGPYARLRPQ
                     AKLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNVEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7090..7362)
                     /locus_tag="ACFM75_000827"
     CDS             complement(7090..7362)
                     /locus_tag="ACFM75_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC6255407.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7387..8067)
                     /locus_tag="ACFM75_000828"
     CDS             complement(7387..8067)
                     /locus_tag="ACFM75_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC6255408.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHALPFIDASVSDMAQLARYAETLSER
                     RQNHYNTP"
     gene            8126..9127
                     /locus_tag="ACFM75_000829"
     CDS             8126..9127
                     /locus_tag="ACFM75_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6255409.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGMGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9221..10069
                     /locus_tag="ACFM75_000830"
     CDS             9221..10069
                     /locus_tag="ACFM75_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC6255410.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10095..10580
                     /locus_tag="ACFM75_000831"
     CDS             10095..10580
                     /locus_tag="ACFM75_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC6255411.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10682..12250)
                     /gene="msrAB"
                     /locus_tag="ACFM75_000832"
     CDS             complement(10682..12250)
                     /gene="msrAB"
                     /locus_tag="ACFM75_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC6255412.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRIEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12396..13661
                     /gene="ftsY"
                     /locus_tag="ACFM75_000833"
     CDS             12396..13661
                     /gene="ftsY"
                     /locus_tag="ACFM75_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC6255413.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     IPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         3018 a         3558 c         4158 g         3181 t
ORIGIN      
        1 aatttgatcc actataattc cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa
       61 aacttcgcct taatgcgccc gattgactag ggatgggctt cagatggcat tgttttccgg
      121 tttacgggcg gtattcgggc ttcataccgt tgggcagaag ctgccagaca tatcccgtgg
      181 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta tccccaaaaa taatccacgc
      241 gcaccgcgcc tttaatcgcg ctgccggtat cctgcgccat aatcaggcgg ttgagggctt
      301 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc acccaaggta atgtagtgcc
      361 ggtcgactgc gccggcatat tcccccatca gcggcgtgcc cagtgcgccg acagggccgt
      421 cattgccgct tccggtaagc tcgcggaaaa agatatagct ggggttttga cccaaaactt
      481 cggcgaggcg ttgcggattt tgccgcatat aagacttaat gccctgcatg gaggtttgtc
      541 cgagtttgag gtagccctta tccgccatat agcgtccgat ggaaacgtag ggatgttcgt
      601 ttttgtcggc atagccgatg cggatgtatt tgccggatgg ggttttcaga cggcccgagc
      661 cttggatgtg cataaaaaaa agttcgacgg ggtcttcggc gtaaccgagt atcggggctt
      721 tgccgtcaag cgcgccgccg ttgatttggt ttcgcgtgtg gtaggggagg aagcggcttc
      781 cttcaaacct gcctttgatt gccgttgtgt gcgcggtgat ggggaatcgg gagaggtcgg
      841 cggtgtgcgt gccgccggca ttgtcgattg tgccgctgtt ttttcccgtc tgcctgatgc
      901 ggacaagggc ttttccgctc cgcaaaccgg caggcagggg gacggagata aaatcgtcgg
      961 gaataccgta aatcgggaag cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg
     1021 gctcgtaata gccggtaacc gtaccggcaa ggcttccgtt gcctgcaacc tgccacggcg
     1081 tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt
     1141 gggcacacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     1201 attgcaggct tttggcgaaa tcctgcgccg cccagtgggg cagggacagg tgcggcacaa
     1261 cggtataaac ggccccgccg ccggcaaccg tcgttccggc gggggcgggg atgccgaccg
     1321 gccggtccgg gccgttgatg atggatgtgt cgggttgcgg aaaggtttgg atgctcttgc
     1381 tttggcaggc ggcgaggatg gcggcggcga tgccgcacag ggcggcgcgg aacaggtatt
     1441 ttttcataat ggacaatgtt gtcagcagta ataaaaaaag atggtttcgg gcggcgttgc
     1501 ggcagccgtg gagaggggat tttaacacag ggcgcagctg cagcctgcgg aactttccgc
     1561 cgcgcggtac tgcagataaa aataacttgc atttgtattt acaagcaatg aaaatattcc
     1621 gataatatat tattcatcat ccttgttcgt tcgcgtttat gctggtcgct tttttaatta
     1681 tgttgcgcga gggtatcgaa gccgcgctca ttgtcggcat cgttgccggt tttctgaaac
     1741 agtccggaca ttccaaactg atgcctaagg tctggttcgg ggtcgtcctt gcttctttga
     1801 tgtgtttggg gctggggtac ggaatccatt cggcaacggg cgagattccc caaaagcagc
     1861 aggagttcgt cgtcggcatt atcggcctgg ttgctgtcgc gatgctgact tatatgattt
     1921 tatggatgaa aaaagccgcc cgttcgatga agcggcagct tcaggattct gtgcaggcgg
     1981 ctttgaaccg tggcagcggt caaggatggg ccttggtcgg tatggcgttt cttgccgtgg
     2041 cgcgcgaagg tctggagagc gtttttttcc tgcttgccgt attcaaacag agcccgacgt
     2101 ggcagatgcc ggccggtgcg gtagcggggg ttttggctgc cgccgtgatt ggcgcgttga
     2161 tttatcaggg cgggatgcgc ctgaatctgg cgaagttttt ccgttggacg ggggcgtttt
     2221 tgattgccgt tgccgccggt ctgcttgccg gctcgctgcg cgcgctgcat gaggcaggta
     2281 tttggaacgc gcttcaggac attgtgttcg actcatcaaa atatttgcac gaagacagcc
     2341 cgttgggcgt gctgctcggc ggatttttcg gctataccga ccatccgacg cagggcgagg
     2401 ccttggtttg gctgctgtac cttattcccg tcataacttg gtttttgtgc ggcagcaggc
     2461 cgtctgaaac tttaacccgt aaagaggagc tgaaatgaga aaattcaatt tgaccgcatt
     2521 gtccgtgatg cttgccttag gtttgaccgc gtgccagccg ccggaggcgg agaaagctgc
     2581 gccggcagcg tccggtgagg cgcaaaccgc caacgagggc ggtgcggtca gtatcgccgt
     2641 caacgacaat gcctgcgaac cgatggaact gaccgtgccg agcggacagg ttgtgttcaa
     2701 tattaaaaac aacagcggcc gcaagctcga atgggaaatc ctgaaaggcg tgatggtggt
     2761 ggacgagcgc gaaaacatcg cccccggact ttccgataaa atgaccgtca ccctgttgcc
     2821 gggcgaatac gaaatgactt gcggtctttt gaccaatccg cgcggcaagc tggtggtaac
     2881 cgacagcggc tttaaagaca ccgccaacga agcggatttg gaaaaactgt cccaaccgct
     2941 cgccgactat aaagcctacg ttcaaggcga ggttaaagag ctggtggcga aaaccaaaac
     3001 ctttaccgaa gccgtcaaag caggcgacat tgaaaaggcg aaatccctgt ttgccgacac
     3061 ccgcgtccat tacgaacgca tcgaaccgat tgccgagctt ttcagcgaac tcgaccccgt
     3121 catcgatgcg cgtgaagacg acttcaaaga cggcgcgaaa gatgccggat ttaccggctt
     3181 ccaccgtatc gaatacgccc tttgggtgga aaaagacgtg tccggcgtga aggaaattgc
     3241 agcgaaactg atgaccgatg tcgaagccct gcaaaaagaa atcgacgcat tggcgtttcc
     3301 tccgggcaag gtggtcggcg gcgcgtccga actgattgaa gaagtggcgg gcagtaaaat
     3361 cagcggcgaa gaagaccggt acagccacac cgatttgagc gacttccaag ccaatgtgga
     3421 cggatctaaa aaaatcgtcg atttgttccg tccgttgatc gagaccaaaa acaaagcctt
     3481 gttggaaaaa accgatacca acttcaaaca ggtcaacgaa attctggcga aataccggac
     3541 taaagacggt tttgaaacct acgacaagct gggcgaagcc gaccgcaaag cgttacaggc
     3601 ctctattaac gcgcttgccg aagaccttgc ccaacttcgc ggcatactcg gcttgaaata
     3661 agccgcaagc gttcagacgg tatttggcgg cagataccgt ctgaagtttt atagtggatt
     3721 aacaaaaacc agtacggcat tgcctcgcct tgccttgccg tactatttat actgtctgcg
     3781 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ccgccatata ttgcagggcg
     3841 ggatttcaac ctgccgctat cggttaatgg aaaaacggcg tgcagggata cccatcctgc
     3901 tgcacggata ttgaaggaaa caccatgagc aaaaaccaac ccgcacaacc gaccaggcgc
     3961 actcttttta aaaccgcgat cgcagccgga gcagtcggcg caatcggagg ttatctcggc
     4021 ggcaaaaaac agggcgaaac cgccgaacgc accgccgaaa gccaacactc gccccaagcc
     4081 tatccctgct acggcgaaca tcaggcaggc atcgttacgc cgcagcaggc gttttcgatt
     4141 atgtgcgcct tcgacgtaac cgcgcaaagt gccaagcagc tggaaaacct gttccgcacg
     4201 ctgaccgccc gcatcgagtt tctcacccaa ggcggcgaat accaagacgg cgacgacaaa
     4261 cttccacccg ccggcagcgg cattttgggc aaagccttca accccgacgg gttgaccgtt
     4321 accgtggggg tgggcagcag cctgtttgac ggcaggttcg gactcaaaga caaaaaaccg
     4381 attcatttgc aggaaatgcg cgacttctcc aacgataagc tgcaaaaaag ctggtgcgac
     4441 ggcgatttga gcctgcaaat ctgtgccttc acccccgaaa cctgccaagc cgccctgcgc
     4501 gacatcatca aacacaccgt ccaaaccgcc gttatccgtt ggagtatcga cgggtggcag
     4561 cccaaatccg aacccggcgc gatggcggcg cgcaacctgt tgggcttcag ggacggcacg
     4621 ggcaacccca aagtttccga tcccaaaact gccgacgagg ttttgtggac gggtgtggcc
     4681 gccaacagcc tcgacgaacc ggagtgggcg aaaaacggca gctatcaggc agtccgcctt
     4741 atccgccact ttgtcgagtt ttgggacagg acgccgcttc aagagcaaac cgacattttc
     4801 gggcggcgca aatacagcgg tgcgccgatg gacggcaaaa aagaagccga ccaaccggat
     4861 tttgccaaag accccgaggg gaataccacg cccaaagaca gccatatacg cctggcgaat
     4921 ccgcgcgatc ccgaattcct caaaaaacac cgcctcttcc gccgcgccta cagctattcg
     4981 cgcggactcg cctcaagcgg acagcttgat gtcgggctgg tgttcgtctg ctatcaggca
     5041 aaccttgccg acggattcat cttcgtgcaa aacctcctta acggcgaacc gctggaagaa
     5101 tacatcagcc ccttcggcgg cggttatttc ttcgttctgc ccggcgtgga aaaaggcgga
     5161 ttcttgggac aagggctgct gggcgtataa atccgcatat caaaaacgcc gtccgaacct
     5221 tgccaaacgg gttcggacgg cgtttcttgt tttggacggt caggcttttt tgacgaattc
     5281 ggattttaaa ttcatcgcgc tgccgtcgat tttgcaggcg atgttgtggt cgccttcctg
     5341 caggcgtatg cctttgacct ttgtaccttg tttgatcacc atcgagctgc cttttacctt
     5401 gaggtctttg atgaggatga cggtgtcgcc gttttgcagc gccgcgccgt tggcatcgcg
     5461 cacttgagcc gcaaggtcgg aggcggattc ggtttcattc cattcgtggg cgcattcggg
     5521 gcagatgtat tgtccgccgt cttcgtaggt gtactcggag gcgcattgcg ggcaaggggg
     5581 taatgacatg gtttgccgtc cttatcggat gtttgttttt gggatgccgt ctgaaacctg
     5641 aaaccggctt cagacggcat ggctttattg tttgtctttt tccggcctca cccagccttc
     5701 gatgacggtt tggcgggcgc gggcgagggc gagtttgttg tcttcgacat tgcgggtaat
     5761 cgtgctgcct gcgcctgtgg ttactttgtt gccgagggta acgggggcga ctaggacgca
     5821 gtttgaaccg atgcgaactt cgtcgccgat gacggttttg tgtttgtgca cgccgtcgta
     5881 gttggcaata atcgtacctg cgccgaagtt ggttttgctg ccgacttcgg cgtcgcctat
     5941 gtaggtgagg tggttggctt tggtgccttt gccgatggcg gcgtttttaa tttcgacgaa
     6001 gttgccgacg tggacatcgt cagccagttt ggcttgcgga cgcaaacgag cgtatgggcc
     6061 gatttggttg ttttcgccga cttcgcagcc ttcgaggtgg gagaaggggg cgattttgct
     6121 gtttgcgccg attttagcgt ttttgatgac gcagttggca ccgatttcga cgttgtcgcc
     6181 gagttcgatt ttgccttcaa agatacagtt cacatcaatt acgacatctt gcccgtgttt
     6241 cagacggcct cgtaaatcga aacgtgccgg gtcgcgcagg gttacgcctg ctttgagcaa
     6301 ttcttgcgcc tgttcagttt ggaagatgcg ttcgagttcg gcgagctgga gtttgttgtt
     6361 cacgccggcg gccagatggg aggagcggac ttggacggga tgaattttga tgccgtcggc
     6421 aacggctttg gcaatgaggt cggttaaata atattcgcct tgggcattgt tgctggaaag
     6481 gctgttcaac caggcttcga gtttggcgtt gggcaggacg aggatgcctg tgttgatttc
     6541 tttcacggct ttttgggcgg cgtcggcatc tttttcttcg acgatggcgg ttacgctgcc
     6601 gctgctgtcg cggatgatgc ggcccaagcc tgtcgggtcg gcgggaacgt cggtcaacag
     6661 gccgacttcg ttgcctgcgg cttcgagcag ggtttcgagg gtttcaacgt caattaaagg
     6721 aacgtcgccg tacaacacca gcgtgcggcc ttcggcggaa aggtggggca gggcggtttt
     6781 gacggcgtgg ccggtgccga gctgttcggt ttgttcaacc cagacgacat cgcgtttgac
     6841 ggtgtctaaa acttgctctt tgccgtggcc gacgacgacg cagatgtttt gcggattcag
     6901 cgcggcggcg gtgtcgataa cgcgcccgag cattgtttcg ccgccgattt cgtgcaaaac
     6961 cttgggcatt ttggaataca tgcgcgtgcc tttgccggcg gcgaggatga cgatgtttaa
     7021 agtgttttgc ggcatagcgt tttcctgtgc aatgccgtct gaagcggctt cagacggcat
     7081 agggtgggtt tatcggtttt gaaatttggg cttttgccag tgttggcgat gctcttcgtc
     7141 ggcgttgttg ccggtttggt tgggtaacac ggcatggcgt tcgggacggt attggttgta
     7201 gttcatattt ttcgagtagc tgccgtcttg gtaataaacg ggcgtgccgg cgggatattt
     7261 ttgacggacg gcggtcttgc cgttgccgtc ttgataagtt tcccacgcgc agcccgacaa
     7321 aagggcggcg gcagtcatca ggataaggaa ggttttacgc ataacattct ttcgtatttt
     7381 cgggggttag ggggtattgt aatgattttg gcggcgttct gacaaagttt ctgcataccg
     7441 agccagttgc gccatatcgc ttacggaggc atcgataaag ggcagcgcgt gggattttgc
     7501 accgaaccgg acggttttca tgcccaatgc ttttgcctga tgcaggttgt ccgcgctgtc
     7561 gtccaccata atgcagcatt cgggcggtac gtccaaaagg cggcagacat tgagatacgc
     7621 ttgcggattg ggtttgtaca gcagtccgaa atcatctgtg ccgaacagtg cgtcgaaacg
     7681 gttttccaaa ccgagtgcgc cgacaacggc acggacgtaa aacgacgggc cgttggaaaa
     7741 aaccgccttg cgcccgttca gacggcatag ggtgttttcc gtatccgcca tgccgtgcag
     7801 cctggtcagg attgcatcga tcggatggct ttcgcgcaaa aattcggcga tgtcgatttc
     7861 gggatggtgg atttgcagtc cggcgagcgt tgcgccgtag cggtgccaat agtcttgacg
     7921 caggtcggac gcggcagatt cggagagttt gaggcggcgt gccatatagc gtgtcatagc
     7981 gcggttgatg agtgtgaaga ggccggcatc ggcattgtgc agcgtgttgt cgaggtcgaa
     8041 cagccacacg gtcgggtttt cttgcatttt gaaccgtgaa aatttgttag aatgttattt
     8101 tacagcgaat agaggaggac tcggaatgaa acggaaaatt tggctgctgc cgctgctggc
     8161 ggtttcggca tacctgcagg cgcagacgga agtcaggctg gcggtgcata agtcgttcag
     8221 cctgcccaaa gggctgattg cgcgcttcga gcgggcaaac gatgcgaagg tgtcgattat
     8281 tcaggcgggc ggcgcgaacg aaatgctcaa caaactgatt ttgagccgcg ccaacccgat
     8341 tgccgacgcg gtgtacggtt tggacaacgc caatatcggc aaggcgcggg aaatgggcat
     8401 tttggcggcg gcgcaacccg aatccgcccc cgtcgcggtc gggctgcctt cggctttggc
     8461 ggtcgattac ggctatgtgt ccatcaatta cgacaaaaaa tggtttgaag gcaaaaagct
     8521 gcccctgccg caaaccctgc aggatttgac ccgccccgaa tataaaaacc tattggtcgt
     8581 gccgtccccc gccacgtcgt ccccggggct gggcttcctg atggcgaaca tcagcgggat
     8641 gggcgaagaa ggcgcgttca aatggtgggc acagatgcgg cagaacggcg tgaaggtcgc
     8701 caaaggctgg agcgaggcat attacaccga cttttcgcac aacggcggcg cgtatccgct
     8761 ggtggtcggt tatgccgcca gcccggcggc ggaagtgtat ttttccaaag gcaaatacag
     8821 cgagccgccg acgggcaacc tgtttttaaa aggcggcgta ttccgccagg tcgaaggcgc
     8881 ggcggtcttg aagggcgcga aacagccgga attggcggca aaactggtgc aatggctgca
     8941 aagtcgggaa gtgcagcagg cggttccgtc cgaaatgtgg gtttaccccg ccgtcaaaaa
     9001 cacgcgcctg cccgacgtgt tccgcttcgc ccaagccccg acgcacacca ccgcccccgc
     9061 gcagcgcgat attgatgcga accagcgcgg atgggtttcc cgttggatta gaacggtttt
     9121 gaaataaaac aaacataccg ccccgcaggg cttcagacgg tatttttaca cctgtgccga
     9181 ttacgccgca cggggcggat gttcaatcaa gaggaaaaca atggacttca aacaatttga
     9241 ttttttacac ctgatcagtg tttccggttg ggagcatctg gctgaaaagg cgtgggcgtt
     9301 cgggctgaac cttgccgccg cgctgcttat ttttttggtc ggaaaatggg cggcgaaacg
     9361 cattgtcgct gtgatgaggg cggcgatgac gcgcgcgcag gtcgatgcca cgctgattag
     9421 ttttttgtgt aatgttgcca atatcggctt attgattttg gtgattattg ccgcattggg
     9481 cagattgggc gtttccacaa catccgtaac cgccttaatc ggcggcgcgg gtttggcggt
     9541 ggcgttgtcc ctgaaagacc agctgtccaa ttttgccgcc ggcgcgctga ttatcctgtt
     9601 ccgcccgttc aaagtcggcg attttatccg cgtcggcggt tttgaaggat atgtccgaga
     9661 gattaaaatg gtgcagactt ctttgcggac gaccgacaac gaagaagtcg tgctgcccaa
     9721 cagcgtggtg atgggcaaca gcatcgtcaa ccgttccaca ctgccgctgt gccgcgccca
     9781 agtgatagtc ggcgtcgatt acaactgcga tttgaaagtg gcgaaagagg cggtgttgaa
     9841 agccgccgtc gaacacccct tgagcgttca aaacgaagag cggcaggccg ccgcctacat
     9901 caccgccttg ggcgacaatg ccatcgaaat cacattatgg gcttgggcaa acgaagcaga
     9961 ccgctggacg ctgcaatgcg acttgaacga acaagtggtc gaaaacctcc gcaaagtcaa
    10021 tatcaacatc ccgttcccgc aacgcgacat acacatcatc aattcttaaa cgccgtctga
    10081 aaggggagtg ggaaatggac gcgctgcaca ccatcgcccg aaacctgacg aaaaaacgtc
    10141 aaaccgtaag ctgtgccgaa tcctgtacgg gcggaatgct tgccgccgca ttcacaagcg
    10201 ttgcaggcag ttcgcaatgg ttcgaccaga gttttgtaac atacagcaac aaagccaaag
    10261 aagaccgctt gggcgtgttg cccgaaaccc tgctcgaaca cggcgcggtc agccgccaaa
    10321 ccgtctatga gatggcgcgc ggcgcgaaag ccgtggcgca ggcggattac gccgtcggta
    10381 tttccggcat cgccggaccg ggcggcggca gcgaaagcaa acccgtcggc acggtttggt
    10441 tcgggtttgc ctttccgggc ggaagttgcg aagcaatgcg ccgttttgac ggcaaccgcg
    10501 aatccgtccg cgcgcaggcg gtcgccttcg cgttggaacg gttggcgggg ctgattgaaa
    10561 acggcggcga tgccgtctaa acaaaatctc cgtctggaca aaatccccgc cggataaaaa
    10621 ataccgtctg aaacgttgca ggtttcagac ggtattttgt cgggttaggc ggcggtgcgg
    10681 cttatttcac tttactcttc aacgcgccgt agcctgccgc gtccatttgt tccagcggga
    10741 tgaatttcaa gctcgcgccg ttgatgcagt agcgcagtcc gcctttgtcg cgcgggccgt
    10801 cggggaagac gtgccccaag tgcgaatcgg cggcgtggct gcgcacttcg atgcggcgca
    10861 tattgaagct gaaatcatcg tgttcggtaa cggattttgc atcaatcggg cgcgtgaagc
    10921 tcggccagcc gcagccggaa tcatatttgt cggcggagct gaacaaaggt tcgccgctga
    10981 caacgtccac ataaatgccg ggtttgaaca aatggtcgta ttcgtggctg aaggcatatt
    11041 cggtcgcgct gttttgggta acttggtatt gctcttcggt cagggtgcgt ttgagttcgg
    11101 cgtcactcgg ttttttatac gttgccgcgt cgaagccttt gccttgcggg gcggtcttgg
    11161 ttttgcccgg cagcggttcg tcggctttgc ggatatcgat gtggcagtag ccgttggggt
    11221 ttttaatcaa gtagtcctga tggtattcct cggcatcgta gaagtttttc agcggctcgt
    11281 tttcaacaac gaggggcagt tggtattttt gctgctcgcg tttgagggcg gcggcgatga
    11341 cggctttttc ggcggggtcg gtgtagtaca cgccgctgcg gtattgcgta ccggtgtcgt
    11401 tgccctgttt gttgaggctg gtcggatcaa cgacgcggaa gaaatattgc aggatgtcgt
    11461 ctaggctgag tttgtcggca tcgtaggtca ctttgacggt ttcggcgtgg cccgtatggc
    11521 ggtaggacac gtcttcatag ctcggatttt tcgtgttgcc gttggcgtag ccggataccg
    11581 cgtcaaccac gccgtcgatg cgttggaaat aggcttccaa gccccagaag cagccgccgg
    11641 cgaggtaaat ggtgcgcgtg ttcatgattt ttgaatcctt tttctgagtg tcgggtttgt
    11701 agaacgaatg tttcaagctg cccaaatcgg cattcgggtc gcggattaac gccaacgcct
    11761 gcgcttcgtt gatgctgcct ttgacgatgc gctgcacgtc gccgtcttta ccgattaacg
    11821 cccacgaggg gtaaacgctg atattcaggc tttgggcgat cgtgccgccg ttgtcggtta
    11881 cgacgggcag cttgggataa ttcaaaccgg cataccattt ttggaagtcg ccgtcttttt
    11941 tctcgtgcaa aaagcccgga gacgcgacgg taatcaggtt ggcggagctg aattttgcat
    12001 cttgcgccca tttttcggtc tgtcccaatt cggacaaaca caaaggacac cagctcgccc
    12061 aaaatttaat cagcgtcggt ttgtcttttt tcaagtaaac actggcgggg cggttgtctg
    12121 cggttttcaa agtggataaa gtgtgcggca cggtcgcggc tccggcatcg acgattttgg
    12181 gcgaacaagc acccagcgca agcaggcagc cgaacttggc gcaaagggaa aagaaagtac
    12241 ggtgtttcat tttaatgttt cctgtgtgga cggtttgcat gattagacgt ttgagatgcc
    12301 gaaaccttac agctcggatt ttcagacagc cttgccgcgt aaaatacgct acaatacgcc
    12361 ctatttcaag tttctaaaat taaaaggaaa attcaatgtt cagcttcttc cgtcgcaaga
    12421 aaaaacagga aacgccggct cccgaggagg ctcaagttca ggaaactgca gcaaaagcag
    12481 aatccgaagt tactcaaata gttgaaaata ttaaagaaga agtcgaatct ttaacggaaa
    12541 gcgtcaaagg gcaggtcgaa tcggctgttg aaaccgtcag cggtgcggtt gaacaagtaa
    12601 aggaagccgt tgccgagatg ctgtctgaag cagaggaagc ggcggaaaaa gcagcggaac
    12661 aagtcgaagc ggcaaaagaa gccattgccg aaaccgtcgg cgaggctgtc gggcaagttc
    12721 aagaagccgt tgcaacaact gaagaacaca agctcggttg ggcggcgcgt ttgaaacaag
    12781 gtttgaccaa atcgcgcgac aaaatggcga aatcgctggc gggcgtgttc ggcggcggac
    12841 aaatcgacga agatttgtac gaagagctgg aaaccgtgct gattaccagc gacatgggca
    12901 tggaagccac cgaatacctg atgaaagacg tgcgcgaccg tgtcagcctc aaagggctga
    12961 aagacggcaa cgaattgcgc ggcgcgttga aagaagcctt gtacgacctg attaagcctc
    13021 tggagaaacc tttggttttg cccgaaacca aagagccgtt tgtcatcatg cttgccggca
    13081 tcaacggcgc gggcaaaacc acgtctatcg gcaaactcgc caaatatttc caagcgcagg
    13141 gcaaatccgt attgctggcg gcaggcgata ctttccgcgc cgccgcgcgt gagcagcttc
    13201 aagcttgggg cgaacgcaac aacgtaaccg tgatttcgca aaccacgggc gattccgccg
    13261 ccgtgtgctt cgatgccgtc caagccgcca aagcgcgcgg catcgacatt gtgcttgccg
    13321 acaccgccgg ccgcctgccc acgcagcttc atttgatgga agaaatcaaa aaagtgaaac
    13381 gcgtattgca aaaagccata cccgacgcgc cgcacgaaat catcgtcgtg ctcgatgcca
    13441 atatcgggca aaacgctgtc aaccaagtca aagcctttga cgacgcattg gggctgaccg
    13501 gcttaatcgt taccaaactc gacggcacgg caaaaggcgg catcctcgcc gcgcttgctt
    13561 ccgaccgccc cgtccccgtc cgctatatcg gcgtgggcga aggcatagac gacctgcgcc
    13621 cgtttgacgc gcgcgcgttt gtggacgcac tgctggattg agccgaaatg ccgtccgaaa
    13681 acagcagacc gatgccgtca ttcccgcgca ggcgggaatc cagaccttgg gataacagca
    13741 atattcaaag gttataaaag acccgtcatt cccgcgcagg cgggaatcta gaccttagaa
    13801 caacagcaat attcaaagat tataaaagac ccgtcattcc cgcgcaggcg ggaatctaga
    13861 ccttagaaca acagtaatat tcaaaggtta gctgaagctt tagagattct ggatt
//
LOCUS       DAXHRI010000037        13443 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXHRI010000037 DAXHRI010000000
VERSION     DAXHRI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.037"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(101..529)
                     /locus_tag="ACFM75_000834"
     CDS             complement(101..529)
                     /locus_tag="ACFM75_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC6255414.1"
                     /translation="MAILKLDKHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLGYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(594..2342)
                     /locus_tag="ACFM75_000835"
     CDS             complement(594..2342)
                     /locus_tag="ACFM75_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC6255415.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLLHRLELTGIPSERRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPNFDRNRLVSDIKKICETELAVFSSSAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPFGMTDADDAYTTLLGLFSHEYFHSWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTVWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKTLYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2669..4102
                     /gene="ccoN"
                     /locus_tag="ACFM75_000836"
     CDS             2669..4102
                     /gene="ccoN"
                     /locus_tag="ACFM75_000836"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC6255416.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4129..4740
                     /gene="ccoO"
                     /locus_tag="ACFM75_000837"
     CDS             4129..4740
                     /gene="ccoO"
                     /locus_tag="ACFM75_000837"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC6255417.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4745..4915
                     /locus_tag="ACFM75_000838"
     CDS             4745..4915
                     /locus_tag="ACFM75_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC6255418.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4939..5850
                     /gene="ccoP"
                     /locus_tag="ACFM75_000839"
                     /pseudo
     CDS             4939..5850
                     /gene="ccoP"
                     /locus_tag="ACFM75_000839"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            5926..6270
                     /locus_tag="ACFM75_000840"
                     /pseudo
     CDS             5926..6270
                     /locus_tag="ACFM75_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            6472..7881
                     /locus_tag="ACFM75_000841"
     CDS             6472..7881
                     /locus_tag="ACFM75_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC6255419.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGAAGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGAAGMVPPAQKIKLPVWWAMAVPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            7982..11191
                     /gene="recC"
                     /locus_tag="ACFM75_000842"
     CDS             7982..11191
                     /gene="recC"
                     /locus_tag="ACFM75_000842"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC6255420.1"
                     /translation="MFYLYQSNRLETLAALFAHIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPASLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYTLECLSDLLESRFEVDKVLALLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAAFLETLTDIVRIWRQPATVDEWVGRCRGLLKTLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQSVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIVAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPFPYAIPSDGQILKGSLSNLYQCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAGKKEEAGAA"
     gene            complement(11259..12827)
                     /gene="mtrF"
                     /locus_tag="ACFM75_000843"
     CDS             complement(11259..12827)
                     /gene="mtrF"
                     /locus_tag="ACFM75_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC6255421.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPLPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13160..13372
                     /locus_tag="ACFM75_000844"
     CDS             13160..13372
                     /locus_tag="ACFM75_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255422.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
BASE COUNT         3385 a         3226 c         3556 g         3276 t
ORIGIN      
        1 cactgtaaaa acggagaagg acagaggacg gcagtaaggt ttgccgtcct ctttttcaag
       61 ttgcggtgga atgccgcttg aaggtgtttt cggcttgtaa tcagacgcgg gcgttgtcca
      121 gccgctctct gaagttttcc aaatttacgc ctgccgcttg ggcgcggcgg atgatttcgt
      181 caaccggcat accttctgcc gcccggcgga agccccacag gagggagcag cgcgtaccgg
      241 tccggcaata gccaaggacg ggatattcgg cttgtccgat gagttggcgg aaggtttcga
      301 catcgtgttt ttggatgtcg cgtgcggtaa cgggttggtg atggaatcca gtaacgcctg
      361 cttgttccag ccactgtttg atttgggcga agtcgggttg cgattcttct tcgcggtcgg
      421 ggcggttgca gatgacggtt ttgatgccca gttgcgcgat ttgttccgca tcggctttgg
      481 tcagttgcgg agaaatatag aggtgtttgt caagtttcag aatggccatt ttgtttcctt
      541 tactttgcgg ttgtttttca gacggcattt tgtgtgcaat gccgtctgaa agtttaaccg
      601 aacaaccagt tgtccaacag gtttcggtct gtgatatgta ggatggcagt atccgctgcc
      661 gctgcctgaa ccgtcaagac ggtttgacgc aatatgccgg cacggaagaa gtggatattg
      721 atttttgcat tgacgtggta tcgggcccat tgtgcggtaa agtcggtgca ggcataaccg
      781 tctaaagcaa tgattttgtc ttgcgggcac agtgccgcag attccgcgct gccgccgttg
      841 aagacatggg tcaggacgat gtggtcggtg ttttgtttga aacgtgcgcc aaaatcgcct
      901 gccgacggga cggggcagat gtgttctgcg tatccgccgc cgtgttgtcg gggaagcggc
      961 aggaaggtca gtcccacgcc tgcggttgcc aggcattcgg caagcggcaa atcttcggta
     1021 ctgtataacg ttttttggaa aaaatcttcc aaatccaagc cggtaatttc ctgacagcgg
     1081 atttgccagt gtttttccgg aatacccgag tgtgtgtccc tccactcccg atagagtttg
     1141 tccattaacg tatcgagaga atgtctgccg ttgcttcggt tgcgtattat cagatcaagg
     1201 cacaatgcgg caagcgcgcc tttctggtag tagctgacga tggcgttggg gctgttttca
     1261 tccggtttgt aaaatttgtt ccacacggta aaactcgatt ccgccaaggt ctgcctcaaa
     1321 cggccgcggg tttgttgtac gcgcgtaatg ccttgtgcca gcaggtttaa ataagattcg
     1381 ggcgagatgg tgcggctgcg tgccaaaaac aaatcgtcgt aataggatgt aatgccttcg
     1441 aatgcccata gttgttcggt atagttttct ttgtcgaggt cataagggac gaacgcggca
     1501 ggtttgatgg atttgacgtt ccacgagtga aaatattcat gggagaaaag tccgagcaat
     1561 gtggtgtagg catcgtcggc atcggtcata ccgaacggcg gaaggctgtg gcggtcggcg
     1621 agcagggcgg tgctgtcggc gtgttccaaa ccgccgtaaa tatggtcgcc gacgtggagc
     1681 aggaacaaat atttttgaaa cggggcagag gaggaaaaca ccgccagttc tgtttcgcag
     1741 atttttttga tatccgaaac cagcctgttg cggtcgaaat tgggatatat gccgcttaag
     1801 gcaattgtgt gcggaatgcc tgccgcctca aaatctaaaa attcaatcaa gcccatctcg
     1861 acaggtcggt caatcaattc ggcataagat gccgcctgaa agacaaacct ccctgtttcc
     1921 ggcagcgttg tggcaatacg cctttcggac ggaatacccg tcaattccaa gcggtgcagc
     1981 agcgtttccg ttccttcgac tttcaaaaac aggcacgatc cgtcaaaaaa accgcgttct
     2041 gtcgtcagga aagaacctcg aaccgacaaa tcgaatgcat atacggtgta gcggatttgc
     2101 cactcgccgc gtacggcggc agcgtgccag cggttttttg caatttgttc gagcggcatg
     2161 gacgtgccgt tacaggatgc atggatagaa gtgatgtggc gggaaaaatc ccgaatcaga
     2221 tagctgcccg gaacccagtt tggcaggctt atttctatag gaagatcatc atcttgtgtg
     2281 aatgtcagca ggatgtgcca ctcgtggtcg agaggggacg gggttatttt ataatgaatc
     2341 ataactatca aatatatcgt taaacgggat ggattaaata cctttaaaat gcagcttaac
     2401 ctacatcaat aaagtgccac aaaaagggaa aattggaaac aaaagcaatg gcggtcgggt
     2461 tgtaatttat tataacaata tgatttatat gtaataaaaa acagattaag caaagggctc
     2521 gccggcggcg cgaatgtaat tccgcttggc gtaataaggc agatatgtta aatttagaaa
     2581 aaattacgct tgcccggatt gcaccttaaa caggctgcgg aagcggcagg ataaaaatct
     2641 ttattcccat aaattcatgg agactctcat ggacacacaa acttacaact acaaagtggt
     2701 gcgccagttc gccatcatga ctgtagtttg ggggattgtg ggtatgttgg tcggcgttat
     2761 cgtcgccgcc cagctttttg ctcctgccct cgacttgtct aatatcggac cttggttcca
     2821 cttcggccgc ctgcgtccgc tgcacaccaa tgcggttatt ttcgcatttg gcggttgcgg
     2881 cctgatcggc acatcatact acgttgtcca acgtacttgt aatacccgtc ttttcggcgg
     2941 ttggctgccg gcatttacct tttggggctg gcaggcggta atcgttgccg ccgtcgtcag
     3001 cttccctatg ggttggactc aaggtaagga atatgccgaa ctggaatggc cgatcgatat
     3061 tctgattact ttggtttggg tggcttacgc catcgtattc ttcggtacga ttgccaaacg
     3121 taagattaaa catatttacg ttgccaactg gttctacggc ggctttattt tggcggtcgc
     3181 acttttacac atcgtcaaca atatcagcat cccggcaggg ttgatgaagt cataccccgt
     3241 ctattccggt gcgattgatg ctatggttca atggtggtac gggcataacg ccgtgggctt
     3301 cttcctgact gccggcttct tgggtatgat gtactatttc gtacccaaac aagcagcccg
     3361 ccccgtttac tcctaccgcc tgtccgtcgt tcacttctgg gcgttgattt tcacctatat
     3421 gtgggcgggt ccgcaccatc ttcactacac tgcgctgcct gactggacgc aatctttggg
     3481 tatggttctg tctttgattc tgttcgcacc ctcttggggc ggtatgatta acggcatcat
     3541 gaccttgtcc ggcgcgtggg acaaactgcg tacagacccg attcttaaat ttctgattgt
     3601 atccttgtcc ttctacggta tgtctacctt tgaaggcccg atgatgtcga ttaaaacggt
     3661 caatgcattg agccactata cggactggac cgtcgcgcac gttcatgcgg gtgcgttggg
     3721 ctgggtaggc tttgtaacca tcggttccgt ctattacatg attccccgtc tgttcggcaa
     3781 agaacagatg cacagcacca agctggtaga agcacatttt tggattgcga ccatcggcgt
     3841 ggttctgtat atcgctgcca tgtggattgc cggtgtgatg cagggtttga tgtggagttc
     3901 tttgaacgat gacggtacgc tgacctactc gtttgtcgaa tccgtaaaac gcaccatgcc
     3961 ttactacgtg atccgttttg caggtggttt attgtatctg agtggtatgt gcattatggc
     4021 gtacaacgtg taccgcacag ccatcggtgg taaagcagtc gatgccgaaa tccctgcggt
     4081 ttcccaaaca cagcaccact aaaatactaa gaaagatagg ctaccaaaat gaaattacaa
     4141 caattggctg aagaaaaaat cggcgttctg attgtgttca cgctgcttgt agtcagtgtc
     4201 ggtctgttga ttgaagttgt gcccttggcc tttaccaagg cggcaacaca gccggcgccg
     4261 ggcgtgaagc cttacaatgc cctgcaggtt gccggacgcg atatttacat ccgtgagggc
     4321 tgttacaact gccactcgca aatgattcgt ccgttccgtg cggaaaccga gcgttacggt
     4381 cattactctg ttgccggaga gtcggtttac gaccatccgt tccaatgggg ttccaaacgt
     4441 actggtcctg atttggcacg tgtgggcggt cgctattccg acgaatggca ccgtatccac
     4501 ctgctgaatc cccgtgatgt cgtgcctgag tccaatatgc cggcattccc gtggcttgca
     4561 cgcaataaag tcgatgtcga tgcaaccgtt gccaacatga aggctttgcg taaagtaggt
     4621 actccttaca gtgatgagga aattgcgaaa gcgcctgagg ctttggcaaa caaatccgag
     4681 ctggatgctg tagtcgccta tctgcaagga ttgggtctgg ctttgaaaaa cgtaaggtaa
     4741 catcatggat attaacggta ttcgtgctct cttcacggta tggatcttta tctgtttcct
     4801 gttggtactc tatatcgtct tcaacaggcg gaataagaaa aactacgata atgccgcaaa
     4861 cagcattttt gatgaaaacc aagatgcgca agataagaaa agcgaaaacc gttaatattg
     4921 tgataacgga gcaaaacaat gaacacaaca tcccaattta ccagtaattt ctggaatata
     4981 tatattgcag ttattgtctt actgagcttt atcgctttgg cttggctgct gctgtctcaa
     5041 aatgttgtca aacgtccgaa gaagggcgaa gaagtacaaa ctacgggtca tgagtgggac
     5101 ggcattgccg aatacgacaa tccgctgccc cgctggtggt tttggctgtg tgttttgacg
     5161 tggctgttcg gtatcggtta tctggttatg tatccgggtg tcggcgacta caaaggtctg
     5221 ctgaaatgga ccagccataa ccaatatgaa aaagaggtca aaaaagccga tgagcaatac
     5281 ggcaaactgt atgccaagtt tgcggatatg ccgattgaaa aagtggcaaa agaccctcaa
     5341 gccaagcaaa tcgcccaaaa cctgtttaac acttattgta tccagtgcca cggctctgat
     5401 gctaaaggct ctaaaggttt tccgaatctg accgatagcg attggttgtg gggcggtgat
     5461 cccgataaaa tccacgagac catcgaaaaa ggccgtgttg cgactatgcc tgcctggggt
     5521 cctgctttgg gcgaagaagg cgtgaaagac gttgcccatt atgtgatgtc cctttcaaaa
     5581 cccgaaggtc agtatgatga ggaacgtgcc gcgcgcggac aagccttgtt cagcggtccg
     5641 cctgccaact gtttcacttg ccacggcgat aagggacaag gtatccaagg tttgggtccg
     5701 aacctgactg atgacgtgtg gttgtggggc ggtacgcaaa aatccattat cgaaaccatt
     5761 accaacggtc gcagcagcca aatgcccgct tggggacatt tcttggataa agacaaactg
     5821 catattatga ctgcttatgt atggggtctt tccgataaag acggtaaagc tccggtgaaa
     5881 aaagccgagc ctgcaccgac tcccgcaccg gcggcagaac ccgcagcctc tgctcctgca
     5941 gaagcagcac aagccgcgtc cgaagccaaa cctgccgcag cagaacctaa agccgaggaa
     6001 aaagctgcac ctgctgccaa agcggacggc aaataggttt atgaaaccgt ttgtgccgcc
     6061 tgccatggca atgcgattcc gggtattccc catgtaggca tcaaagccga ttgggccgac
     6121 cgcatcaaaa aaggcaagga cacgttgcac aaacacgcga tcgaaggttt caatacgatg
     6181 cccgcaaaag gcggtcgcgg cgatttgagc gatgatgagg ttaaagctgc ggttgactat
     6241 atggtcaacc agtcaggcgg caaattctaa cttgactgag tttccgatat tatcggttat
     6301 gaaaatccag attcagtatt gaatctggat tttttgtttt aacgggcatc cggttttaaa
     6361 cgcagactgc ctatttgttg taatgaattt tgactttagt cataaagaat tagtctttat
     6421 tgtttataat cgcgcatcat cacgatagcc ctattttcaa aaggacgatt aatggataca
     6481 caaatcaaaa ctgaagccgg tcagcagaat aaccggcgtt atctgaccgt ttggcggtgg
     6541 catttttatg ccggtctgct ggttatgcct tttcttaccc tgcttgccgt tacgggtttg
     6601 ggtatgctgc tgtttgccaa tattaccggt aaggagggcg agcggattca tgttgtgccg
     6661 caggcaacgg tacaacctct gtctgttcag gcggaagcgg cacgcagtgc cgttaatccg
     6721 gaaacttcgt ccgtcgttca gtatattgcg ccgcgtgccg atgatatggt tgccgtgttc
     6781 cgtgtcaaca atgagggcaa ggcaacgatg gtcgcggtcg atccttatac ggcaaaagtg
     6841 gtcagtacca tgccgcgcaa tcagggttgg tattacgcga tggatgaaat ccacagcgat
     6901 atgatgctcg gtgcggcagg tgattatctt ttggaaacgg ctgcatcgct gacgattatc
     6961 atgataatca gcggtttgta cctttggtgg gtgaaacggc gcggcatcaa ggcgatgctg
     7021 ctgccgccaa aaggcagggc gcgttcttgg tggcggaatc tgcacggcgc gtttggaact
     7081 tgggtgtcgt tgattttact gttgttctgc ctgtcgggta ttgcttgggc aggtatttgg
     7141 ggcggcaagt tcgtgcaggc ttggagtcag ttcccggcag gcaaatgggg tgtcgaaccg
     7201 aaccctgttt cagtcgtgcc gacccatggc gaggtattga acgacggcaa ggttaaggaa
     7261 gtgccgtgga ttttggagct tacgcctatg cctgtttcag ggacgactgt gggcaaagac
     7321 ggcattaacc ccgacgagcc gatgacattg gaaaccgtcg accgttttgc gcgggaaatc
     7381 ggtttcaaag ggcgttatca gttgaatttg cccaaaggcg aggacggcgt atggactttg
     7441 tcgcaagact ctatgagtta cgatatgatt agtccgtttg ccgaccgcac ggtgcatatc
     7501 gaccagtaca gcggcaagat tcttgccgat atccgttttg atgattacaa cccgttcggc
     7561 aaatttatgg cggcgagcat tgcgctgcat atggggactt tgggctggtg gagcgtgttg
     7621 gcgaacgtcg tgttctgcct tgccgtcatt tttatcggta tcagcggctg cgtgatgtgg
     7681 tggaaacgcc gtccgtccgg agcggcgggc atggttccgc cggcacaaaa aatcaagctg
     7741 cccgtctggt gggcaatggc ggtgccgctg ctgctgattg cattgctttt cccgaccgcg
     7801 ctgcttgcca ttgccgtgat ttggctgttg gatacgctgc tgctgtcgcg gattcctgtt
     7861 ttgaggaaat ggtttaaatg accaaatgcc gtctgaaagg ttcagacggc attttgtttg
     7921 aaggcaggcg ggggaaaggc tatataatcc cgaatacttt tccgcaactt ttgtcgaaat
     7981 catgttttat ctgtatcaat ccaaccgtct tgaaacgctg gcggcactgt ttgcccacat
     8041 tcaaaaagtc aaaccgctga aatcggcttt acagcccgaa cagattattg tgcagagtca
     8101 ggggatgcgc cgctacctca acacctgcct cgcccgcgat ttgggcgtgg cggcgaattt
     8161 ggcgttcagc ctgcccgcca gcctgacgtg gaagctgatg aaaaaactga ttcccggcat
     8221 tccggaactc agcccgtttg cacccgaagt catgcgctgg cggctgctgg atttgttccg
     8281 cagcgaagca ttccggaata cggcagaatt tgaagatgtg aggaatgtgc tgcaagacta
     8341 tctaggcagc ggcgaatcgg cagattacca gcttgcggga cagcttgcgg acatattcga
     8401 ccaatacctt gtctatcgtc cgcagtggat agacgcgtgg cagcagggca ggatactcag
     8461 tttgggcgac gatgaaatct ggcagtccaa actgtggcgt tatcttgatg acggcaggca
     8521 gagcgcgccg caccgtgttg cgttgtggga aaagctgttg gaatctttga gcagtgataa
     8581 gctgcccgag cgttatttcg tgttcggcat ttccacgatg gcgccgatgt atttgcagct
     8641 tttgcacaag ctgtccgaac attgcgatgt gttcgtgttt gcactcaatc cgagcgggat
     8701 gtattggggc aacgtcatcg aagcggcgca aatcctcaaa ggcggcggcg atcccgattt
     8761 aactcaggca gggcatccgc tgctcgcctc attgggcaag caggggcgcg actttttcga
     8821 ctttttgaac gaaatggaaa tagaaggcga aacgccggta tttgaagaag gcggacgcga
     8881 tacgcttttg cacgccctgc aaaccgatat tcaaaacctg aaaatgccgt ctgaaaatgt
     8941 gggaagcgtc aacacgggcg acggctcgat acgcatcgtc tccgcacaca gccccttgcg
     9001 cgaattgcag atactcaaag acaagctgtt gaaaattctg catgaacatc ccgattggca
     9061 gccgcatgat atcgccgtat taaccccgaa catcgaatcc tatacgcctt ttatcgaagc
     9121 cgtgttcgga caggcgcagc ccggtgcgca ggcattgccg tattccgttt cagacgtgaa
     9181 aatcagccgc cgacaaccgt ttttttatac attggaatgc ctgtccgact tgttggaaag
     9241 ccgatttgaa gtcgataaag tgctcgcgct tttggaaacc gcgcccgtat tgcgccgttt
     9301 cggactgact gaggacgatt tgccgctttt gcacgatatg gttgccgatt tgaacgttca
     9361 ctggggtttg gacggagaaa tgcgcggcgg cacggatcag ctgttcacct ggaagcaggc
     9421 ggtagaacgc atgatattgg gctggatgct gcccgaaggc ggcaacccga tgtggcagga
     9481 tgtcagcgcg tggtatgccg acgtgaacca aaccgccctg ttcggacgtt ttgccgcctt
     9541 cctcgaaacc ctgacggata ttgtacggat atggcggcag cccgcaacgg ttgatgaatg
     9601 ggtaggacgt tgccgaggtt tgcttaaaac attgttccaa gccgaagccg atgaccaaaa
     9661 ggcagtccaa aacctcgaaa acgaatgggt caaatggcag gcggaaaccg aattggcgca
     9721 attttccgga cagttgccgc cgcacaccgt tatccgccac atccgccgct ttctcgacag
     9781 cgaaagcgag gcaggctttt tacgcggcgg catcaccttt tgcagtatgg tgccgatgcg
     9841 gagcctgccc tttaaagtca tctgtctgtt gggtttgaac gacggagatt ttccccgcaa
     9901 taccaaagcc gccgtattcg acctgattgc taaacatccc gccaaaggcg accgcgcccg
     9961 ccgcgacgac gaccgctacc tcttcctcga agccctcatc agcgcgcgtg aaatcctgta
    10021 cctgtcctac atcgggcgcg acatccgcaa agacgaagag cttgctccgt cttcgctgtt
    10081 gggcgaactc atcgacacag ttgccgctat ggcgggcatc ggtagccgcc aacttgcaca
    10141 aaactggata gaacagcatc cgctgcaagc cttctcgcgc cgatatttcc aagaaggcgg
    10201 acgttcagac ggcatattcg gcacgcgtac cgactacgcc gccgcgctcg gacaaacgcc
    10261 cgaaccgccg caaccctttt tcgaccaatc cgtagaaaac gccgaacctg ttgccgaaat
    10321 cggacaggac gaatttatcc gtttctggcg caatcccgtc aaagtatggc ttcagcagca
    10381 gcttgcgtgg agcgaacccc atatcggcga agcctgggag cctgccgaac ccttcgagcc
    10441 gcaacacgcc gatcaaatcg ccgaaatcta catcgaagca cggcgcgaag gacgggattt
    10501 tgcccaaacc gccgcccgca tcgttgcgga aagtctcctg ccgtcgggag agttgggcag
    10561 actttggcag caggacttcc aaaccgctgc caaacaaatc gacacggcgg ttttaaacag
    10621 ccccaaactt cctccgttcc catacgccat accgtcggac gggcaaatcc tgaaaggcag
    10681 tttgagcaat ttataccaat gcggacaagt gttttacgcc tacggcaaac ccaatgcgcc
    10741 gcaacgtatc gcttttctgc tggaacacct gatattttgc gccgttatgc cgtctgaagc
    10801 cgaaacgcgg caaaccttta tcgtccaatc cggagaaacc gaaatattgg cggaaatcgc
    10861 gcaagacagg gcattgcagc tattgtcgga atggatggca tttttcaata tcgggcaaaa
    10921 ccgcccgctg ccgttttttg ccaaaacctc gcttgccgcc gccgaagcgt ttgcccaaaa
    10981 acaagattgg gaagctgccc tgaaaaaagc ccaaaccgcc taccacggca acaaagtcag
    11041 caaagggcag aaagactata ccgaagtcgc cctcgtgttc ggcaacgcaa gccaaaaccc
    11101 actcgaacag cccctgtttg aaaatctcgt ccgtctgctt gccgacacgc ttgccgcagc
    11161 gggaaaaaag gaagaggccg gagcagcctg acgggataga aaggcaaatg ccgcctgaaa
    11221 gcgtttaagc atttcagacg gcattttgtt tatcgtgttt aaggtgcggg atagaatgtg
    11281 ggcgcgccgg gaccgacggg caggcccaaa acaaataccc aaatgcagaa taaggcaatc
    11341 cacgcaatca agaagaaagc ggaatacggc aacatcatag aaatcagcgt acccacgccc
    11401 gcatcttttt tgtatttgat caccgtcgcc ataatcagcc cgaaataact catcatcggc
    11461 gtaataatat tggtaacgga atcaccgatg cggtaagcgg cttgaatgac ttcgggcgcg
    11521 tagccggcca acatcagcat agggacgaaa atcggcgcag ttaccgccca ttgcgcggag
    11581 gcggagccta tcatcagatt gataaaagca caaattaaaa taaaaccgat aaacaacacg
    11641 ctgccgccca agccgacttc ttttaagaac gtcgcccctt taacggcaat atattgccca
    11701 atattcgtcc aattaaaaaa tgcgacaaac tgtgcggcaa aaaagatgat gaccaaataa
    11761 agccccagag tactcatcga ttcggccatc gcattaacga cttcctgttc gccgcgcaaa
    11821 cttcgggtta cccggccata aacaatgccc ggcagtgcaa acaacaagaa aataaaaaca
    11881 acaatcgatt ttaaaaacgg cgaaccggaa accaatcctg tttcaggatg acgcaaaata
    11941 ccgtcggcag ggacgatgct ccaagccaat agggcggata aggcaacaaa caccacgcca
    12001 gcccaaatta atcctttata ttccaaaggc gtgatttcat tggaatgtcg aatgtctttt
    12061 tcttcttgtg acaaatctga ttgataaggg cccaattgcg gttcgacgat tttttcagta
    12121 acaaaataac caatcaaagc aatcacaaac gtactggcta ccataaaaaa ccagttggct
    12181 tcagggccta cgacgtagtc gggatggatg atttgcgccg cctgttgggt gatgcctgcc
    12241 aagagcggat cgattgtgcc taagaacaga ttggccgaat aaccgcccga aacgccggcg
    12301 aaagccgcag ccagaccggc aagcggatgg cggccgaggg aatgaaagat gatggcggac
    12361 aaagggatta ggacgacata gcccaattca gaagcggtat tagataaaat ccctgtaaaa
    12421 acaaccataa aagtagtgag tttgcgtgga gattttgtga gcaataagcg cattaatgcg
    12481 gaaatcaagc ccgatttttc cgcaatcccc acgcccaata aagaaaccaa caccgttccc
    12541 aacggcgcga aaccggtgaa atttttaacg gtatgcgtca ggattttgat caaaccgtca
    12601 gcatcgagca ggctgacaac gtgaatcaaa ccgtcatcgg cacgtccttt cgcaccaaca
    12661 gggagcggat cggggacgga tagtccgaaa tacgcaccgg cggcagaggc aatcagcaat
    12721 aacacaatga aaataataaa aagcgtaacc gggtgcggca acatattgcc cagccattcg
    12781 actgtgcgta aaaatcgtcc gtcccgttgc gtatcggttt gactcatctg cttctcctta
    12841 aaaaaatata ttgagtgcat taaatttttg aatgtaacat gaagtgtttt gtaagcaaac
    12901 taaaataatc gtatatgtat ataaccgtaa tagattaaaa cagaaaatgc ccacatcgtt
    12961 attctcataa aggcggtaat ccgaaaactt gacgttaaag ctttatcagg aatgactgga
    13021 actcaaaaaa acggattccc gcctgcgcgg gaatgacggg attttcggtt tttgtttgca
    13081 agaatgccgg agaagaacgc agtttacatt ttgaatggta ttgttattaa ttgtaaataa
    13141 atattggaag gtaaatgata tgaaaaatga aattcaaaaa atcatggaca aatataatcc
    13201 atggcatgaa gatgattttg agtcgtatga agacattgcc aaagatgtat cgctgatgac
    13261 agataaaacg ttcattgaac attatttgtt agaagtttat tcagaagaaa acggacattt
    13321 tgaccaagag aatatccatg cgatgatagg ggaaattaaa aatgcaattt agtgaatatg
    13381 agacctttgc aaaaatagtc tgttaacgaa atttgacgca taaaaatgcg ccaaaaaatt
    13441 ttc
//
LOCUS       DAXHRI010000038        12927 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXHRI010000038 DAXHRI010000000
VERSION     DAXHRI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.038"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            78..153
                     /locus_tag="ACFM75_000845"
     tRNA            78..153
                     /locus_tag="ACFM75_000845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Thr,seq:cgt)
     gene            229..1356
                     /locus_tag="ACFM75_000846"
     CDS             229..1356
                     /locus_tag="ACFM75_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC6255423.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            1537..2094
                     /gene="frr"
                     /locus_tag="ACFM75_000847"
     CDS             1537..2094
                     /gene="frr"
                     /locus_tag="ACFM75_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC6255424.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPASVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            2150..2896
                     /locus_tag="ACFM75_000848"
     CDS             2150..2896
                     /locus_tag="ACFM75_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC6255425.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFSRQILKGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGNAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            2899..3696
                     /locus_tag="ACFM75_000849"
     CDS             2899..3696
                     /locus_tag="ACFM75_000849"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC6255426.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMSGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GSWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFCGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            3701..4936
                     /gene="ispC"
                     /locus_tag="ACFM75_000850"
     CDS             3701..4936
                     /gene="ispC"
                     /locus_tag="ACFM75_000850"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC6255427.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYTGRLNEHGIASIILTASGGPFLTADLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDSSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            4971..6311
                     /gene="rseP"
                     /locus_tag="ACFM75_000851"
     CDS             4971..6311
                     /gene="rseP"
                     /locus_tag="ACFM75_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC6255428.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKTGDKLTAADGKPITSWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            6368..8761
                     /gene="bamA"
                     /locus_tag="ACFM75_000852"
     CDS             6368..8761
                     /gene="bamA"
                     /locus_tag="ACFM75_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC6255429.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKQYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            8827..9327
                     /locus_tag="ACFM75_000853"
     CDS             8827..9327
                     /locus_tag="ACFM75_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC6255430.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            9360..10406
                     /gene="lpxD"
                     /locus_tag="ACFM75_000854"
     CDS             9360..10406
                     /gene="lpxD"
                     /locus_tag="ACFM75_000854"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC6255431.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            10440..10889
                     /gene="fabZ"
                     /locus_tag="ACFM75_000855"
     CDS             10440..10889
                     /gene="fabZ"
                     /locus_tag="ACFM75_000855"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC6255432.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVNGQVAVEAVIMCAKRVV"
     gene            11096..11776
                     /locus_tag="ACFM75_000856"
     CDS             11096..11776
                     /locus_tag="ACFM75_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC6255433.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            11819..12595
                     /gene="lpxA"
                     /locus_tag="ACFM75_000857"
     CDS             11819..12595
                     /gene="lpxA"
                     /locus_tag="ACFM75_000857"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC6255434.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKNVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSTRGIIR"
BASE COUNT         3270 a         3905 c         3224 g         2528 t
ORIGIN      
        1 cactataccg cgcactgcct tgccgcccgc cagaaaagtt gcacaaacaa ccgttcatat
       61 atatcataac gaaaaacgcc ggtgtagctc agtcggtaga gcagcgcatt cgtaacgcga
      121 aggtcggggg ttcgattccc ttctccggca ccaataccaa gcacagaccc tcccttcctc
      181 gggaagcctg tgctttttca catttccgct tcagacggca caaccgatat gaacacctcg
      241 caacgcaacc gcctcgtcag ccgctggctc aactcctacg aacgctaccg ctaccgccgc
      301 ctcatccacg ccgtccggct cggcggggcc gtcctgttcg ccaccgcctc cgcccggctg
      361 ctccacctcc aacacggcga gtggataggg atgaccgtct tcgtcgtcct cggcatgctc
      421 cagtttcaag gggcgattta ctccaaggcg gtggaacgta tgctcggcac ggtcatcggg
      481 ctgggcgcgg gtttgggcgt tttatggctg aaccagcatt atttccacgg caacctcctc
      541 ttctacctca ccgtcggcac ggcaagcgca ctagccggct gggcggcggt cggcaaaaac
      601 ggctacgtcc ctatgctggc ggggctgacg atgtgcatgc tcatcggcga caacggcagc
      661 gaatggttcg acagcggcct gatgcgcgcg atgaacgtcc tcatcggcgc ggccatcgcc
      721 atcgccgccg ccaaactgct gccgctgaaa tccacactga tgtggcgttt catgcttgcc
      781 gacaacctga ccgactgcag caaaatgatt gccgaaatca gcaacggcag gcgcatgacc
      841 cgcgaacgcc tcgaagagaa catggcgaaa atgcgccaaa tcaacgcacg catggtcaaa
      901 agccgcagcc acctcgccgc cacatcgggc gaaagccgca tcagccccgc catgatggaa
      961 gccatgcagc acgcccaccg taaaattgtc aacaccaccg agctgctcct gaccaccgcc
     1021 gccaagctgc aatctcccaa actcaacggc agcgaaatcc ggctgcttga ccgccacttc
     1081 acactgctcc aaaccgacct gcaacaaacc gtcgccctta tcaacggcag acacgcccgc
     1141 cgcatccgca tcgacaccgc catcaacccc gaactggaag ccctcgccga acacctccac
     1201 taccaatggc agggcttcct ctggctcagc accaatatgc gtcaggaaat ttccgccctc
     1261 gtcatcctgc tgcaacgcac ccgccgcaaa tggctggatg cccacgaacg ccaacacctg
     1321 cgccaaagcc tgcttgaaac acgggaacac ggctgacggt cggacacgat gccgtctgaa
     1381 acgcctgccg cttcagacgg catctccaat ccgcctgcct tgtcgtcatg cccgaataac
     1441 ggtactattc gcagttaaca gttttcatgc cgtctgaaag gtttgaagcg cgtttcagac
     1501 ggctgcccgc ctaccttatc ttaccgaaag aacaatatga tcaacgatat tcaaaaaaca
     1561 gccgaaggca agatgcagcg ttcggtcgaa gtactgaaag aaaatctggc gaaagtgcgt
     1621 accggccgcg cgcataccgg cctgctcgac caagtggaag tcgaatactg gggcagtatg
     1681 gtccccgtca gccaagttgc caacgtaacg cttctggacg cgcgcaccat cggcgtgaaa
     1741 ccgtttgagg gcaatatggc ggccaaagtc gagaaagcca tccgcgattc aaacttgggg
     1801 ctgaaccctg catccgtcgg cgacctgatc cgcgtgccga tgcccatgct gaccgaggaa
     1861 cgccgcaaag acctgattaa agtcgtacgc ggcgaagcgg aagaaggccg cgtctctatc
     1921 cgcaacgtgc gccgcgatgc caacgaccac atcaaaaaac tcctcaaaga caaagaaatt
     1981 tccgaagacg aggcacgtcg cggcgaagaa gcggttcaaa aactgaccga caaatacatt
     2041 accgaagccg acaaactcct gactgccaaa gaagaagatt tgatggcaat ttaacctgca
     2101 cggttcggcg ttcagacggc atttgaacgc cgaaccgcga aaggcagaca tgaaaagcag
     2161 cacgcaggcc gttttggaac acaccgccat tcccaagcat atcgccgtga ttatggacgg
     2221 caacggccgt tgggcgaaaa aacgtttcct cccgcgcata atgggacaca aacgcggttt
     2281 ggacgcattg gaaaatatgg tgaagcattg cgccaaactg ggtgtgcaat atctgaccgt
     2341 gtttgccttt tcaaccgaaa actggcgccg ccccgaagac gaagtttcgt tcctgatggg
     2401 gctgttttta caggctttgc aaaaacaggt acgccgtctg cacgaaaaca atatgcgcct
     2461 aaaaatactg ggcagccgcg aacgcttcag ccgtcaaatc ctgaagggca tcgaagaagc
     2521 ggaggcgttg acggcaaaca ataccggtct gaccctgagc attgccgccg actacggcgg
     2581 ccgctgggat attttgcagg cggcaaacaa actgattgcc gaaggcgtat ccgagattac
     2641 ggaagacacg ctggcgaaac acttgatgct gggcaatgcg ccggaaccgg atttgttcat
     2701 ccgcactggc ggcgaaacgc gcatcagcaa tttcctgctc tggcagatgg catatgcaga
     2761 actgtatttc accgacatcc tgtggcctga tttcgacgaa accgccttag atgccgccgt
     2821 cgcttcgttc caaaaacgcg aacggcggtt cggacgcacc tccgagcaac tgcctatcga
     2881 acagcaaagg aactgaatat gctgaaacaa cgggtgataa ctgctatgtg gctgctgccg
     2941 ctgatgctgg gcatgctgtt ttacgcgccg caatggttgt gggctgcatt ttgcggactg
     3001 attgccctga ttgccttgtg ggaatatgcc cgtatgagcg gcttgtgcaa aatcaaaacc
     3061 aaccattacc tcgccgcaac tttggttttc ggcgtggttg cctatgcggg cggctggatg
     3121 ctgcctaatt tggtttggta tgttgttttg gcattttggc tcgccatcat gcctttatgg
     3181 ttgagattca aatggaggct caacggcagt tggcaggttt atgccgtcgg ctggcttctg
     3241 gttatgccgt tttggttcgc gctcgtatcc ctgcgcccgc atcccgatga tgccctgccg
     3301 ctgctcgccg tcatgggcct ggtctgggtt gccgatgttt gcgcatattt ctgcggcaag
     3361 gcgttcggta aacacaaaat cgcaccggca atcagccctg gcaaaagctg ggaaggcgca
     3421 atcggcggcg cggtttgcgt ggccgtgtac atgaccgccg tacgaagtgc cggctggctg
     3481 gcattcgata caggctggtt cgataccgtg ttaatcggtt tggtgttgac cgttgtcagc
     3541 gtatgcggcg accttttgga aagctggctc aagcgcgcgg caggcatcaa agacagcagc
     3601 aagctgctgc ccggacacgg cggcgtgttc gaccgtaccg acagcctgat tgccgttatc
     3661 agcgtctatg cagcgatgat gtcggtttta aattgattct atgccgtctg aaaacgcttc
     3721 agacggcatc cggtataaag ttatccccat tatgacacca caagtcctga ccatattagg
     3781 cagtaccggc agcataggcg aaagcacgct ggacgttgtc tcccgccacc ccgaaaaatt
     3841 ccgcgtattc gcgctggcag ggcataagca ggtcgagaaa ctggcggctc aatgtcaaac
     3901 gtttcgcccc gaatatgccg tcgttgccga tgccgaatac gccgcccggc ttgaagccct
     3961 gttgaaacgc gacggcacgg cgactcaggt tttacacggc gcgcaggcat tggttgacgt
     4021 tgcctctgcc gacgaagtca gcggtgtcat gtgcgccatc gtcggggcgg tggggctgcc
     4081 ttccgcgctc gcagcggcgc aaaaaggcaa aaccatttat ctggcgaaca aagagacgct
     4141 ggtggtttcc ggcgcgttgt ttatggaaac cgcccgtgca aacggcgcgg cagtgctgcc
     4201 cgtcgacagc gaacacaacg ccgttttcca agttttgccg cgcgattaca caggtcgcct
     4261 gaacgaacac ggcatcgctt cgattatcct gaccgcttcc ggcggcccgt ttctgaccgc
     4321 cgatttaaac acgttcgaca gcattacgcc cgaccaagcg gtcaaacacc ccaattggcg
     4381 tatgggacgc aaaatctccg tcgattccgc caccatgatg aacaaaggtt tggagctgat
     4441 tgaagcgcat tggctgttca actgtccgcc cgacaaactc gaagtcgtca tccatccgca
     4501 atccgtgata cacagcatgg tgcgctaccg cgacagctcc gtgttggcgc aactgggcaa
     4561 tcccgatatg cgaacgccca tcgcttattg tttgggtttg cccgagcgca tcgattcggg
     4621 tgtcggcgac ctggatttcg acgcattgtc cgcgctgacc ttccaaaagc ccgactttga
     4681 ccgcttcccc tgcctgaagc tcgcctatga agccatgaac gcaggcggag ccgcgccctg
     4741 cgtattgaac gccgccaacg aagccgccgt cgccgccttt ttggacggac agattaagtt
     4801 taccgacatt gccaaaaccg tcgcccattg tctttcacaa gacttttcag acggcatagg
     4861 cgacataggg gggctcttgg cgcaagatgc ccggacacgc gcacaagcgc gggcatttat
     4921 cggcacactg cgctgatgcc gtctgaacac cgttatcaaa ggaaaaccat ttgcacaccc
     4981 ttctagcttt tatcttcgcc atcctgattt tggtcagcct gcacgagttc ggacactaca
     5041 tcgttgccag attgtgcggc gtcaaagtcg tacgcttttc cgtcggcttc ggcaaaccgt
     5101 ttttcacccg aaagcgcggc gacaccgaat ggtgcctcgc cccgattccg ttgggcggtt
     5161 acgtcaaaat ggtcgacacg cgcgaaggcg aagtatcaga agccgattta ccctacgctt
     5221 ttgacaaaca acaccccgcc aagcgcatcg ccatcgtcgc cgccggccca ctgaccaacc
     5281 tcgcactggc ggttttgctg tacggactga gcttttcctt cggcgtaacc gaactgcgcc
     5341 cctacgtcgg cacagtcgaa cctgacacca ttgccgcccg cgccggcttc caaagcggcg
     5401 acaaaataca atccgtcaac ggcacacccg ttgcagattg gggcagcgcg caaaccgaaa
     5461 tcgtcctcaa cctcgaagcc ggcaaagtcg ccgtcggcgt tcagacggca tcgggcgcgc
     5521 aaaccgtccg caccatcgat gccgcaggca cgccggaagc cggtaaaatc gcaaaaaacc
     5581 aaggctacat cggactgatg ccctttaaaa tcacaaccgt tgccggcggc gtggaaaaag
     5641 gcagccccgc cgaaaaagca ggcttaaaaa caggcgacaa actgactgcc gccgacggca
     5701 aacccattac ctcatggcaa gaatgggcaa acctgacccg ccaaagcccc ggcaaaaaaa
     5761 tcaccctgaa ctacgaacgc gccggacaaa cccataccgc cgacatccgc cccgatactg
     5821 tcgaacagcc cgaccacacc ctgatcgggc gcgtcggcct ccgtccgcag ccggacaggg
     5881 cgtgggacgc gcaaatccgc cgcagctacc gtccgtctgt tatccgcgca ttcggcatgg
     5941 gctgggaaaa aaccgtttcc cactcgtgga caaccctcaa atttttcggc aaactaatca
     6001 gcggcaacgc ctccgtcagc catatttccg ggccgctgac cattgccgac attgccggac
     6061 agtccgccga actcggcttg caaagttatt tggaattttt ggcactggtc agcatcagcc
     6121 tcggcgtgct gaacctgctg cccgtccccg ttttggacgg cggccacctc gtgttttata
     6181 ctgccgaatg gatacgcggc aaacctttgg gcgaacgcgt ccaaaacatc ggtttgcgct
     6241 tcgggcttgc cctcatgatg ctgatgatgg cggtcgcctt cttcaacgac gttacccggc
     6301 tgctcggtta gattttacgt ttcggaatgc cgtctgaaac cgcattccgc accacaagga
     6361 actgacaatg aaactgaaac agattgcttc cgcactgatg gtcttgggca tatcgccttt
     6421 ggcacttgcc gacttcacca tccaagacat ccgcgtcgaa ggcttgcagc gtaccgagcc
     6481 gagtaccgta ttcaactacc tgcccgtcaa agtcggcgac acctacaacg acacacacgg
     6541 cagtgccatc atcaaaagcc tgtacgccac cggtttcttt gacgacgtac gcgtcgaaac
     6601 tgcggacggg cagctcctgc tgaccgttat cgaacgcccc accatcggct cgctcaacat
     6661 caccggcgca aaaatgctgc aaaacgacgc cattaagaaa aacctcgaat cgttcgggct
     6721 ggcgcagtcg caatacttta atcaggcgac actcaatcag gcagtcgccg gcctgaaaga
     6781 agaatacctc gggcgcggca aactcaatat ccaaatcacg cccaaagtaa ccaaactcgc
     6841 ccgcaaccgc gtcgacatcg acatcacgat tgacgagggc aaatccgcca aaatcaccga
     6901 catcgaattt gaaggcaacc aagtctattc cgaccgcaaa ctgatgcggc agatgtcgct
     6961 gaccgaaggc ggcatttgga catggctgac acgaagcaac caattcaacg agcagaaatt
     7021 tgcccaagac atggaaaaag taaccgactt ctaccagaac aacggctact tcgatttccg
     7081 catcctcgat accgacatcc aaaccaacga agacaaaacc aagcagacca tcaaaatcac
     7141 cgtccacgaa ggcggacgtt tccgttgggg caaagtctcc atcgaaggcg acaccaacga
     7201 agtccccaaa gccgaactgg aaaaactgct gaccatgaag cccggcaaat ggtacgaacg
     7261 ccagcagatg accgccgttt tgggtgagat tcagaaccgc atgggctcgg caggctacgc
     7321 atacagcgaa atcagcgtac agccgctgcc aaacgccgaa accaaaaccg tcgatttcgt
     7381 cctgcacatc gaaccgggcc ggaaaatcta cgtcaacgaa atccacatca ccggcaacaa
     7441 caaaacccgc gacgaagtcg tgcgccgcga attgcgccaa atggaatccg cgccttacga
     7501 cacctccaag ctgcaacgct ccaaagagcg cgtcgagctt ttgggctact tcgacaacgt
     7561 acagtttgat gccgtcccgc ttgccggcac acccgacaaa gtcgatttga acatgagcct
     7621 gaccgaacgt tccaccggct cgctcgactt gagcgcgggc tgggtacagg ataccggcct
     7681 ggtcatgtcc gcaggcgttt cccaagacaa cctgttcggt acgggcaagt cggccgccct
     7741 gcgcgcctca cgaagcaaaa ccacgctcaa cggctcgctg tcgtttaccg acccgtactt
     7801 cacggcagac ggggtcagcc taggctacga tgtttacgga aaagccttcg acccgcgcaa
     7861 agcatcgacc agcatcaaac aatataaaac caccacggca ggcgcaggca tccgcatgag
     7921 cgtgcctgtt accgaatacg accgcgtgaa tttcggtttg gtggcagaac acctgaccgt
     7981 caacacctac aacaaagcgc ccaaacacta tgccgacttt atcaaacaat acggcaaaac
     8041 cgacggcaca gacggcagct tcaaaggctg gctgtacaaa ggtaccgtcg gctgggggcg
     8101 caacaaaacc gacagcgcgt tatggccgac gcgcggctac ctgacgggcg tgaacgccga
     8161 aatcgccctg cccggcagca aactgcaata ctactccgcc acccacaacc aaacctggtt
     8221 cttccccctg agcaaaacct tcacactgat gcttggcggc gaaatcggct atgccaacgg
     8281 ctacggcaaa accaaagaaa tccccttctt tgaaaacttc tacggcggcg gcctgggttc
     8341 ggtacgaggt tacgaaagcg gcacgctcgg tccgaaagtc tatgacgaat acggcgaaaa
     8401 aatcagctac ggcggcaaca aaaaagccaa cgtctccgcc gagctgctct tcccgatgcc
     8461 cggcgcgaaa gacgcgcgca ccgtccgcct gagcctgttt gccgacgcag gcagcgtgtg
     8521 ggacggcaaa acctacgacg acaacagcag ttccgcgacc ggcggcaggg ttcaaaacat
     8581 ttacggcgcc ggcaataccc ataaatccac ctttaccaac gaattgcgct attccgccgg
     8641 cggcgcggtt acctggctct cgcctttagg cccgatgaaa ttcagctacg cctacccgct
     8701 gaagaaaaaa ccggaagacg aaatccaacg cttccaattc caactcggca cgacgttcta
     8761 atcccgcaaa tgccgtctga agcccttcag acggcatttc gcggcaacat tcgaaggagt
     8821 tttaccatga cccgtttgac ccgcgcgttt gccgcggctc tgatcggttt gtgctgcacc
     8881 gcaggcgcgc acgccgacac cttccaaaaa atcggcttta tcaacaccga gcgcatctac
     8941 ctcgaatcca agcaggcgcg caagattcaa aaaacgctgg acagcgaatt ttccgctcgt
     9001 caggacgaat tgcaaaaact gcaacgcgaa ggtctggatt tggaaaggca gcttgccgaa
     9061 ggcaaactca gaaacgcaaa aaaggcgcaa gccgaagaaa aatggcgcgg gctggtcgca
     9121 gcgttccgca aaaaacaggc gcagtttgaa gaagactaca acctccgccg caacgaagag
     9181 tttgcctccc tccagcaaaa cgccaaccgc gtcatcgtca aaatcgccaa acaggaaggt
     9241 tacgatgtca ttttgcagaa cgtgatttac gtcaacaccc aatacgacgt taccgacagc
     9301 gtcattaaag aaatgaacgc ccgctgaccc tttcagacgg cataccgaac aggaaaacca
     9361 tgattccggc cacctacacc ctgtcccaaa tcaccgcgcg gctcggcggc gaatggcgcg
     9421 gcgaggacat ttccgttacc gccgtgcgcc cgctcgcaga cgcgcaggcg gaacacatca
     9481 gcttccttgc caatccgaaa tacaaagccg aagtccacga cagcagcgcg ggcgcggtca
     9541 tcgtttccgc caaagcggca gacggatttg aagggcgcaa cctgattgtc gccgacgacc
     9601 cctatctcta tttcgccaaa gtcgcccgcc tgttttcacc cgtcgtcaaa gcgcgcggcg
     9661 gcatccatcc gaccgccgtc gtcgaaccga gtgccaccgt tcccgcaagc tgcgaaatcg
     9721 gcgcgaacgc ctacatcggc gcaaacaccg tgctcggcga aggctgccgc atcttggcaa
     9781 acgccgtcgt ccaacacgat tgcaaactgg gcgacgaagt cgtcctgcat cccaacgccg
     9841 tcgtttatta cggctgcaca ctgggcagac gcgtcgaaat ccacagcggc gcggtcatcg
     9901 gcgcggacgg tttcggactc gcttttgccg gcgattcatg gtttaaaatc ccgcaaaccg
     9961 gcgcggtaac gctgggcgac gacgtagaaa tcggctcgaa caccaacatc gaccgcggcg
    10021 cgatgagcga caccaccgtc ggcaacggca ccaaaatcga caaccaagtc caaatcggac
    10081 acaactgcaa aatcggttcg cacaccgtca tcgccgccaa aaccggcatc tcaggcagcg
    10141 taaccatagg cagctactgc atcatcggcg gcggcgtcgg tacggtcgga cacatcgaaa
    10201 tcgccgacaa aaccaccatc ggcggcggca cgtccgtcac ccacagcatt accgaaagcg
    10261 gcaaacacct cgccggcatc ttcccgatgt ccacccataa agaatgggcg cgcaacgctg
    10321 tttacatcca ccgcttaagc gaaatgaaca aacggctcaa aacactggag cagcagcttt
    10381 cagatgccgg tcaagacagc aaataaccaa accgacttta ttcaaggaat acgacagaca
    10441 tggacgtaca actccccatc gaagccaaag acatccaaaa actcatcccc caccgctatc
    10501 cgtttctcca gctcgaccgc atcaccgcct tcgagccgat gaaaaccctg accgcgatta
    10561 aaaacgtcac cataaacgag ccgcagttcc aaggccattt ccccgacctg cccgtgatgc
    10621 ccggcgtact catcatcgaa gcgatggcgc aggcgtgcgg cacgttggcg attttgagcg
    10681 aaggcggacg caaagaaaac gaattcttct tcttcgccgg catagacgaa gcccgtttca
    10741 aacgccaagt catccctggc gaccaactcg tctttgaagt cgagctgctg accagtcggc
    10801 gcggcatcgg caaattcaac gccgttgcca aagtaaacgg gcaagttgcc gtcgaagccg
    10861 tgattatgtg cgccaaacgc gtggtttgag cgttcggaaa aaggtcgtct gaaagtttca
    10921 gacgacctaa taaaaagaaa acccaccggt ttaggtttat tcaaacccag ggacacggtg
    10981 gggattagtt ttacctaact gtatcaaaga tagtttgaaa cgtcaattta aaaataaaga
    11041 atcaggtgtt agcgtgaatt ataaggaatg ctgtagtatt ttttctgatt tcagaatgga
    11101 acggtataaa aatgccgttg gggaagacaa agcagctgaa ttgtatttat tgaatttatc
    11161 tttatcaaga gaactttttc atgttgtttc aatatttgag attgttttaa gaaacaaaat
    11221 tgacatttgc cttcagcagg cgtttaaaga cagaaactgg ttatataaca gcatacagcc
    11281 acagacgaat cccgcattaa aatatcaagg ttgcttttta agaaatggca cgaaagaatc
    11341 tgctgaatta atcaaggttg cattatctaa aatacaaaat aatagcgggg gaaagtttga
    11401 ccacaaccaa ttggttgcag gactaggatt cggtttttgg cggtatctgt ttgcaggagg
    11461 gaaagatgcc caatttgatg ctacagggaa agtattgatg aaagtttttc caaaaaagcc
    11521 caaatctaca ccaagtgtgc aatataacca aaaatggatt tttcgagaac tttccaatat
    11581 taacaatttc cgtaatcgtt tggcacatca cgaaccgatt tgttttaaag gtgcaataaa
    11641 agataccaac tacgctagaa atattcacca atctattttt gaactgctta attacatgga
    11701 tgtagatact gcttcagtat ttagccattt tagcgatcaa gtgattgcgg tctgcgatga
    11761 aatcgacaaa ctgtgatttt ctattatttg tcttaacaag cagcggagta ttcaaaacat
    11821 gaccctcatc cacccgaccg ccgtcatcga ccccaaagcc gaactcgact ccagcgtcaa
    11881 agtcggcgcg tacaccgtca tcggccccaa cgtccaaatc ggcgcgaata ccgaaatcgg
    11941 cccgcacgcc gtcatcaacg gccacaccag catcggcgaa aacaaccgca ttttccaatt
    12001 tgccagcctc ggcgaaatcc cgcaggacaa aaaataccgc gacgagccga ccaagctgat
    12061 tatcggcaac ggcaacacca tccgcgaatt caccaccttt aatttaggca cggtaaccgg
    12121 catcggcgaa acccgtatcg gcgacgacaa ctggattatg gcgtactgcc acctcgcgca
    12181 cgactgcgtg gtcggcaacc acaccatctt cgccaacaac gcctcgcttg ccggacacgt
    12241 taccatcggc gactacgtcg ttttgggcgg ctacacgctg gttttccaat tctgccacat
    12301 cggcgactac gccatgaccg cgtttgccgc aggcgtacac aaagacgtac cgccctactt
    12361 tatggcatcg ggctaccgcg ccgaaccggc ggggctcaac agcgaaggca tgcgccgcaa
    12421 cggttttacc gcagaacaga tttccgccgt caaaaacgtg tacaaaaccc tctaccatcg
    12481 cggcattccg tttgaagagg ccaaggcaga catcctccgc cgcgccgaaa cccaagccga
    12541 gcttgccgta ttccgagact ttttcgcaca atcgacgcgc ggcatcatcc gctgaccgta
    12601 cccttttgat gccgtctgaa accccgacag cgtttcagac ggcatcggtt tttttaaagc
    12661 ggtatattgt ttgatcgatg catcattttg tagatttcag aagtatggca gtcggcagca
    12721 ttgatgattt ctgccgcccg ccacatcccc attcggacat ttcattgtga cctccgccgc
    12781 tgcttttaaa gaattacaaa caatttccgc agcttgaacc ggcctgtttt cggcgaaaaa
    12841 aaccaaaagt atttttcaaa agaacatatt gtcttattgg atattctgtg ctgaaattac
    12901 tggacactac aaaagtggtg ttttaaa
//
LOCUS       DAXHRI010000039        12904 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXHRI010000039 DAXHRI010000000
VERSION     DAXHRI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.039"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..303)
                     /locus_tag="ACFM75_000858"
     CDS             complement(<1..303)
                     /locus_tag="ACFM75_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6255435.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDY"
     gene            complement(425..1175)
                     /locus_tag="ACFM75_000859"
     CDS             complement(join(425..953,952..1175))
                     /locus_tag="ACFM75_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1301 family transposase"
                     /protein_id="HFC6255436.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQNFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAKLN"
     gene            complement(1375..3009)
                     /locus_tag="ACFM75_000860"
     CDS             complement(1375..3009)
                     /locus_tag="ACFM75_000860"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC6255437.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            complement(3121..4791)
                     /locus_tag="ACFM75_000861"
     CDS             complement(3121..4791)
                     /locus_tag="ACFM75_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC6255438.1"
                     /translation="MEKIWLDSYEQGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGAAAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            complement(4863..5966)
                     /gene="mnmA"
                     /locus_tag="ACFM75_000862"
     CDS             complement(4863..5966)
                     /gene="mnmA"
                     /locus_tag="ACFM75_000862"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC6255439.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            complement(6092..6565)
                     /locus_tag="ACFM75_000863"
     CDS             complement(6092..6565)
                     /locus_tag="ACFM75_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC6255440.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            complement(6680..7063)
                     /locus_tag="ACFM75_000864"
     CDS             complement(6680..7063)
                     /locus_tag="ACFM75_000864"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC6255441.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            complement(7375..8331)
                     /gene="gshB"
                     /locus_tag="ACFM75_000865"
     CDS             complement(7375..8331)
                     /gene="gshB"
                     /locus_tag="ACFM75_000865"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC6255442.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDRRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            complement(8424..10112)
                     /locus_tag="ACFM75_000866"
     CDS             complement(8424..10112)
                     /locus_tag="ACFM75_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC6255443.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            complement(10224..10997)
                     /locus_tag="ACFM75_000867"
     CDS             complement(10224..10997)
                     /locus_tag="ACFM75_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC6255444.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEGGSLKRHHGGASSGGNLPEGLPASRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            complement(11040..11942)
                     /locus_tag="ACFM75_000868"
     CDS             complement(11040..11942)
                     /locus_tag="ACFM75_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC6255445.1"
                     /translation="MDSLMTLISVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGTNLLAFAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGLSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            complement(12091..12807)
                     /locus_tag="ACFM75_000869"
     CDS             complement(12091..12807)
                     /locus_tag="ACFM75_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6255446.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
BASE COUNT         2839 a         3347 c         3446 g         3272 t
ORIGIN      
        1 gtaatcaggg atgctcagtt cgtcaaaacg gcaaaacagg ttgaaatcga tgcgggtgat
       61 gaggctgtgt tcgagttcgg gatcggagag gctgtgccat tgtccgagca ggacggcttt
      121 gaacatggac aacaggggat aggcgggacg gccgcggtgg tctctaaggt aacgggtttt
      181 ttgacggttc aggtattgtt cgatcggctg ccaatcaatc acctggtcca acttcaatag
      241 cgggaagcgg tcgatgtgtt tggcaatcat ggcttgggcg gtttgccgga agaaggtgct
      301 catgagaaat cccctaaatg tcttggtggg aatttagggg attttgggga attttgcaaa
      361 ggtctcaact tgagtttcac accccgctta acaatattca gttggtcaat attagggcgt
      421 gtcctcaatt taacttagca tgaataatag agcaagccaa gtaaagcatc gatttaaaat
      481 tacgagctaa tttctcatag cgagtagcaa tactacgaaa ctgctttaat cgtgcaaatg
      541 cattctcgac taagtggcgt aatttataaa ggtagctatc aaaatcaggg ttaggtttct
      601 tggcattttt acgctttggt ataatagctt tcataccgtg ttctatcgct ttatctctga
      661 tttcttgcga gtcataccct ttgtcagcga taaaatactg ggcttcttgt atgacttcta
      721 tcaagtcgtt tgcaacttga ccgtcgtgca cgttaccccc agtgacttta aaatcgagcg
      781 gatttccatg cgagtccaca cataggtgta tttttgtcgt gtttccgcca cggctttgtc
      841 caattgctct atcgaaacca cgccgagctc cacttgcatg ttgatgacac cgtacataac
      901 ttccgtcgat gaatacccat tctttgtcaa tttcttttcg tagatcaaaa aaaattctgc
      961 cacaagcctt ttttagacca tcggttaaag cggttataag cggttttcca tgaccctagc
     1021 tcaataggta tgtctcgcca tggtgcacct gttcttagct tccatagtat ggcttccatc
     1081 acggtacggt cgttcttcca ttgatgacag ccgtgcgctt tcatggttgt ttgtaattgt
     1141 tcccatatgt tgtcagttat tgcggttctc gccattgttt gtgtttgcct atatttcgtt
     1201 ggaatatagg gttaaaatag ggctcttttg ggcttattca aattagggac acgccctaga
     1261 taaaaccata aaaattaaat tgatggcttt tataatcccc gatttgcgaa aatgccgtct
     1321 gaaagtcttc attcaggctt tcagacggca ttttgatcat caagtaacgc tttatcaggc
     1381 ttttttattg ttcaacgcag ctttgacaaa cgcggtgaac aaaggatgcc ctttgcgcgg
     1441 attggaggta aactcggggt ggaactggca ggcgaagaac caaggatggt tcggcagttc
     1501 gatggtttcg accaagcgtt cgcgtccggc agatacaccg ccgatgacca aacctgcctg
     1561 ttccagtgta ggaacgtagt tgttgttgac ttcgtagcgg tggcggtggc gttcgcggat
     1621 atgtccgctg ccgtagattt tggcggcgag gctgcctgct ttcaattcga cttcttgcgc
     1681 gcccaaacgc atggtgccgc ccaaatcggc ggattcgtcg cgggtttcga cgctgccgtc
     1741 ggcagtttgc cattcgtcaa tcagggcaac gacgggggcg gcgcatttga ggtcgaactc
     1801 ggtggaattc gcacctttca aacctgccac gtcgcgggcg tattcgatca gcgcaatctg
     1861 cataccgagg cagatgccca agtatggcac gttgttttcg cgggcatagc gcacagcggc
     1921 gattttgcct tccacgccgc gcgaaccaaa accgccggga acgaggatgg cgtccatatc
     1981 tttgagcata gaaacgtcgc ccttgttttt ctcgatgctt tcgctgtcaa caaaggtaat
     2041 ttgtacgtcg gtttcggtgt gaatgcctgc gtgtttcaag gcttcaatca gtgatttgta
     2101 ggattcggtc aaatcaacgt atttgccgac catcgcgatt ttgacggtgt gtttcgggtt
     2161 ttggatggcg tggacgattt ttttccacgc ggtcaaatcc gcctgctgca cattaagctg
     2221 caactgctcg gtaatgatgt tgtcgatgcc ttggtcgtgc agcatttcgg ggcattcgta
     2281 gatgctgtcc acatcgtagc tgccgacaat cgcgcgttct tccacgttgc agaacaaggc
     2341 gattttgcgg cgttcgtccg caggcattgt cctgtccata cggcaaatca ggatgtcggg
     2401 ttgcaaaccg atgctcaaca tttctttaac ggtgtgctgg gtcggcttgg ttttgatttc
     2461 gcctgcagcg gcgatgtagg ggacgtagct caagtgggcg aacagggtgt tgttgcgccc
     2521 caactggctt cgcatctggc ggatggcttc caaaaacggc agcgattcga tgtcgccgac
     2581 cgtgccgccg atttcgacaa tcgccacatc gtaacctgct gcgccttcat ggatgcggcg
     2641 tttgatttcg tcggtaatgt gcggaatgac ttgaaccgtg ccgcccaaat agtcgccccg
     2701 tcgttctttg gcgataacgt tttcgtacac ctgccccgtg ctgaagctgt tgcggcgggt
     2761 catcgtggaa tcgatgaaac gttcgtagtg tcctaagtcg aggtcggttt ccgcgccgtc
     2821 gtcggttacg aacacttcgc cgtgttgaaa cgggctcatc gtgccgggat cgacgttgat
     2881 ataaggatca agcttgagca tggtaacgtt caagccgcgc gattcgagga tagcggcaat
     2941 agaagcggcg gcgatacctt tacccagtga ggagacaacg ccgccggtga cgaaaatgaa
     3001 tttggtcatg atggaatacc catgttggaa tgtgcgattt taacttgaag cgcgcggttc
     3061 tggcaaacgg acggatgccg tctgaacggt ggacggctgt tttcagacgg catcttttct
     3121 ttatttcccg gtactttgcc gcaactcgcg gcgcaggatt ttgccgacgt tggacttggg
     3181 caactcgtcg cggaattcga tatttttcgg tactttatat gcggttaatt cggtgcggca
     3241 aaaggcgata agttcttctt tggtcaatga cgggtctttt ttgacgacga atactttgag
     3301 tgcctcgccg gttttttcgt cgggaacgcc gatacaggcg acttccatga ctttgccgtg
     3361 atgcgcgatg acttcctcga tttcgttcgg ataaacattg aatccggaaa caacgacgag
     3421 gtctttctta cgatcgacca gcttcaacca gcctttttcg tccatgacgg caatatcgcc
     3481 ggtttccaag aagccgcgcg cgtctatggc tttggcggtt tcttcggggc ggttccagta
     3541 gccttgcatc acttgagggc cttttaccca caattcgccc ggctgcccga cggggacttc
     3601 tttgccgttt gcgtcgcgca gttcgacttc ggtggacgag acgggcaaac cgatgctgcc
     3661 gctgtatgat tcgatgttta aggggttgca gcacacgccg gggctggctt cggtcaaacc
     3721 gtaggcttcg acgatgggcg tgccggtgat ttttttccat ttttcggcaa cggctttttg
     3781 ggtcgccata ccgccgccca aagtcagccg caattctgaa aaatcgactt cggcaaaatc
     3841 gggccggtta accatcgcgt taaacagcgt gtttacgccg ataaatacat taacccgctg
     3901 ttttttcagt tcgccgataa agcctttcat atcgcgcggg ttggtaatca ggacaatttt
     3961 cgagccggca ttggcaaaaa tcatcagatt cacggttaag gcaaaaatat gatacagcgg
     4021 cagggcggcg ataacggttt ctttgccctc gcgcaactgg tttttaatcc attcttttgc
     4081 ctgaagcata ttggcgcaga tgttgccgtg actcagcacc gcccctttgg caacacctgt
     4141 cgtgccgccc gtgtattgca acagcgcggt atcttcgcgg tttaatgcga caggttggaa
     4201 aacgtgcttc gccccttctt tcaatgccgt ctgaaaggaa acggtttccc gaatacggta
     4261 ttcgggcacc attttcttga ttttccggat gatgaaattg atcagcgaac ctttaagcag
     4321 cccgaacatt tcgccgacag aggcgacgat gacgtgtttg atctgcgtgc gcggcagcac
     4381 cagctccagc gtgttggcga aattttccaa aacgatgatg gcggccgcgc cgctgtcttt
     4441 caactgatgc tccagctcgc gcggggtata gagcggattg gtgttcaccg ctaccaaacc
     4501 tgcctgcaaa ataccgaaaa gggcaatcgg atattgcaat acgttcggca tcataatggc
     4561 aacacgttcg ccgcgcggca gcttgaggac gttttgcaga taagaggcga aatcggtcgc
     4621 cagtttgccg gtttcggcat aagtcagcgt tttgcccatg ttttgaaaag caggttggcc
     4681 ggcaaatttc ttgacacttt ggcaaaatac atcgctgacg gaaccgtatt gcgtgatgtc
     4741 gatttcggca ttcacgccct gttcgtaact gtctaaccag attttttcca taggtatcgg
     4801 tcttttaaag tggaattgag cggaacaatg ccgtctgaaa actgtttcag acggcataag
     4861 ttttatcgtg tgatgatgac aggcttgtcg gtcgtttgaa tgatgccgcc gcccaaacag
     4921 atgtcgccgt cgtacagcac ggcagactgg cccggcgtaa ccgcccattg cggttcgtca
     4981 aacaccagct cggcggtttc ttcatccaaa tagcgcaact cacaaggcgc gtccgccata
     5041 cggtaacgcg ttttgcaggt atagcgtcct gccttcgggc gttcgggaag cgtgaaactc
     5101 aaatcgttca tcacaaggct gcgggtatag agcagcggat ggtcgtgtcc ttgcacgaca
     5161 atcagttcgt ttttcgtcaa atctttagcc gcaacaaacc acggttcgcc cgcgccgccg
     5221 atgcccaaac ctttgcgctg tccgagcgtg tagaacatca gcccgacgtg ttcgccgacg
     5281 gttttccctt cgggcgtaac cattttaccg ttgtcggtcg gcaggtattt ttgcagaaac
     5341 tcgcgaaacg ggcgttcgcc gatgaagcag atgcccgtgc tgtctttttt ggcggcggtc
     5401 ggcaaattaa attcggcggc aaggcggcgg acttcaggtt tttccaaacc gcccaacgga
     5461 aaaatcgcgc gctcgagttg gaaaggcttg aggcggtaga ggaaatagct ttggtctttg
     5521 tttcgatcca aacctttgag caggtaatgc acgccgttgc ggacttcttt gcgcgcatag
     5581 tgtccggtgg cgatggtatc cgcgccctgc cctacggcgt aatccaaaaa gcatttgaat
     5641 ttgatttcgg cgttgcacaa cacatcggga ttcggcgtgc gccccgcact gtattcctga
     5701 agaaaataag caaagacttt gtctttatat tgcgcggcga aattaacgat gtcgatgtcg
     5761 atgccgataa tatcggcaac ggcgatggca tcgaacgaat cctgtttgat gctgcaatat
     5821 tcgtcgttgt cgtcgtcttc ccagttctgc atgaacacac cgcgcacttg ataaccctgc
     5881 tgcttgagca gggcggcggt tacggaagaa tcgacaccgc cggagagccc gacgatgata
     5941 ttggaagggt ttgctgtcgt attcatgcgt agaatatggt tggaaacggc ggtttttaaa
     6001 ggcagatttt aacacatttt aaaggcggtc ataaaaatgc cgtctgaaag cccgggcttt
     6061 ttcagacggc atttcaaata ttttcagcag attagtgctg atgcgcttcg ccgtggtgat
     6121 gaccgtggtc cattgccgac atcggcgcga ttttgacttc cagttggacg gtttgcgctt
     6181 tggcgttttt aaatttcagg gtaacgggaa tcttgtcgcc ctcttttaat tgttttttca
     6241 aacccataaa catcacgtga tagctgccgg gcttgagttc ggtaacggat ttcgcctcca
     6301 aaggcacgcc gcctttgact tcgcgcatcc gcatcacgcc gttgtcgttg atgtgggtat
     6361 gcacttcgac gcggtcggca acggggctgc ttccgccgag caaaaagtct tgtttggctt
     6421 cgtcgttgtg gattttcatg aacgcgccgc ctattttcat accttcgacg gtggtgcgcg
     6481 cccagccgtc ctcaacgtgg actccggcgg cggaaaccgc gcctgccaaa cctgccatca
     6541 tcacggccgc caataatttt ttcatctttc tgctccttat aatatcagac ggggaatgtg
     6601 cttaatctta tagtggatta agtgaataat aaataccaca tactaatcct aaaggattac
     6661 aaatcctgct gcaagcgttt tacccgaaca gggcagacag ccaaaccgcc gccaacatca
     6721 gcatcgcgaa caattgtgcg gcagaacctg cgtctttggc gcgtttggcc agctcgtgtt
     6781 tttcggtcga agtatgatcg acggcagctt cgacggcggt gttgaacagt tcgacaatga
     6841 ccgacacaaa agacgcgata atcaacggca ggcggacggc ggtttcggaa acccaaaaaa
     6901 atgccgcgca caccagcagt acgttcagcc acaaaacctg acggaatgcc gcttcgtaac
     6961 ggtaggcggc ggcgatgccg tctatcgaat agccgaatgc gttaatgacg cgcctgatgc
     7021 cgcctttgcc ttttttttct gccgcgtagg aggaaggttc catcggtatc ctttcaaaat
     7081 gttctcaata tagtggatta acaaaaacct gtacggcgtt gccccgcctt agctcaaaga
     7141 gaacgattct ctaaggtgct gaagcaccga gtgaatcggt tccgtactat ttgtactgtc
     7201 tgcagcttcg ccgccttgtc ctgatttttg ttaatccact atatataccg tctgaaaacg
     7261 gggcggcggg gtgtccgtac ggcattaacc gcatccctgc cggctgagag aaaaccctgc
     7321 ctgcccgaac aaaccaaacg gttgtgaagc aaaagccttt caaacggcat cggtttaacg
     7381 taccgaccac gcggcaacgg catcggcaaa cattgccgcc acgtcgaaac ctttttgttt
     7441 cataatttct tggaatccgg tcgggctggt tacgttgact tcggtcaggt tgctgccgat
     7501 aacgtccaaa ccggccagca ggatgccgcg ccgtttgagt tcgggggcga gcgtttcggc
     7561 aatttcgcgg tcgcgtctgt ccaattcctg cgccacgccg cgcccgcctg ccgcaaggtt
     7621 gccgcgtgtt tcgccgtttt gcgggatacg cgccaaagca taggggacga cttcgccgcc
     7681 gataatcagg atgcgtttgt caccgtgtac gatttcggga atgtagcgtt gcgccataat
     7741 ggtgcgggaa tcaagccgca tcagggtttc gaggatgctg ccgatgttgg ggtctttttc
     7801 ggtcaggcgg aaaataccca taccgcccat accgtccagc ggtttgatga taatgtcgcc
     7861 gtgttctttc aaaaatgcgc ggacatcggc ggaacgggtc gttaccagcg tgggcgcggt
     7921 aaagcggctg aagttcaaaa tcgccagttt ttcattgaag tcacgcatcg actgtccgct
     7981 gttaaagact ttcgcgccct gctgttccgc cagcgtcagt aattgggtgg cgtagaggta
     8041 ttgcatatcg aacggcggat cggtacgcat aatcacggca tcaaatgctt tcaatgccgt
     8101 ctgaactttg tcggcggcgg caaaccagtt tttgtcgtgg tcgtcttgtg cgccgagaaa
     8161 ctcaaaagca gacgcatcag caaccaccag accgttttta accgacaatt ccccgctcaa
     8221 ggtatgaaac agccgccaac cgcgttttgc catctcgcgc atcatggcgt aggtggtgtc
     8281 tttgtaggtt ttgaaacttg ccatcgggtc ggcgataaag aggactttca tcatatttcc
     8341 tttccggtgt gccgaatgtg ccgcatttcg cgggcaaagg ggaaatgccg tccgaacaat
     8401 attcagacgg caaggatggg gttttactta ggctgccaag aatctttcag cgtgaccgtg
     8461 cggttaaaca ccggcgtgtc tttgccgtgg tctttacggt cggttacgaa gtagccgata
     8521 cgctcgaact gccaacggct ttctgccggc aaatctttgg cggcaggttc ggcgtaggcg
     8581 gtgatttcct tgacggattc cggattgagg aaatcggtga acggcaggta ttcgccgtct
     8641 tcgccgcgca cggcatcggg acgctcgacg gtaaagaggc ggtcgtacag acggactttg
     8701 atttcggcgg cgtgttcggc ggaaacccaa tgaatcacgc ctttaacttt acggccttct
     8761 ggatttttgc ccaaggtgtc gtggtcgatg ctgcatttga gttcaaccac attgcctgct
     8821 tcgtctttga cgacttcatc gcacttgatg acatagccgt ggcgcaagcg tacttcgccg
     8881 ccgggaatca ggcgtttgaa gcctttgggc ggattttcgg caaagtcgtc ggcttcgatg
     8941 taaatggttt gtgaaacagg tacttcgcga tcgcccattt cttcgtggtt cggatggaac
     9001 gcggcacggc ggctttgggt tctgccggtt tcaaagttgg tcagggttac tttgagcggg
     9061 ttcaacaccg ccatcaggcg tggggcggaa ttttccaact cttcgcgaat cgcgccttcc
     9121 aacacgctca tatcgacgat gttttcagat ttggaaatac cggcgcgttt ggcaaacagg
     9181 cgcagccctt cgggcgtgta gccgcgtcgg cgcataccgg aaatggtcgg catacgcgga
     9241 tcgtcccagc cggaaacgtg tttttccaca accaactgat tcaatttccg tttggaggta
     9301 atggtgtaca aaagctccaa gcgtgaaaac tcgtattggc gcggacgtgt ggcgtgcggc
     9361 gcggggatgt tgtccaacac ccagtcatac agcggacgat gggcttcaaa ttcgagcgta
     9421 cacaaggaat gcgtgatgcc ttcgatggca tcggaaatac aatgcgtgta gtcgtacatc
     9481 ggatagatgc accatttgtc gccggtgttg tggtgatggg cgcggcggat gcggtagatg
     9541 accggatcgc gcatattgat gttgccggat gccatgtcga ttttcaggcg cagggttttg
     9601 ctgccgtcgg ggaactcacc gtttttcatg cgcgtgaaca ggtcgaggtt ttcttcgata
     9661 ctgcggtcgc gataagggct gtttttgcct gcttcggtca gcgtaccgcg gtattcgcgc
     9721 atttcttctg gcgtcaaatc atcgacatac gctttgccgt ctttaaccaa accgacggcg
     9781 tagtcataaa gctggtcgaa atagttggaa gcgaaacgcg gctcgcccgc ccaatggaaa
     9841 ccgagccact caacgtcttc tttgatggcg ttgacgtatt cgtcgttttc tttttcgggg
     9901 ttggtatcgt caaaacgcag gttgcacaag ccgtcgtaaa tatacgccaa accgaagttc
     9961 aggcagatgg atttggcgtg tccgatgtgc aggtagccgt tgggctccgg cggaaaacgg
    10021 gtttggatgg tttcgtgttt gccgcttttg aggtcttctt cgatgaaggt gcggataaaa
    10081 tggttgtccg caaattggtc tttattgagc atagttttct ttgaacagat ggcttcagac
    10141 ggcattgggg cggttcctta tgccgtctga agcggtttga tgatgtgttt attgtacccg
    10201 acttgcgcgc tttgacatag cgttcagacg gcatcggcaa tcaggcattc cacccccgcc
    10261 tctttcagca tcttctgcat cgcggtatcg ggcagccggt cggtaaatac tttgtcaaac
    10321 gccgtaatgt cgccgagcct gaccagcgcg ttgctgcgga atttactgtg gtccacgccg
    10381 aggaagcgga cgcgcgcatt ggcaatcatc gcctgcatca cgctgacttc tttgtagtcg
    10441 tcgtccaaaa gcgaaccgtc gctttccacg ccgtgcgtac tcatcacggc ataatcgact
    10501 ttgaactggt tgataaaatc gacggttgcc acgccggtaa taccgccgtc caaagggcgg
    10561 acgactccgg aagtgataat gaccgtgtaa tccgtccgcg ccgaaacgat ggaggcgacg
    10621 tggatattgt tggtaataat ccgcaggctg ccgcgccgcc tgaccagctc cgatgccacg
    10681 gcttccatcg tcgtgccgat actgacaaac agcgacgaac cgtcggggat gtgttccgca
    10741 atcagccggg caatggcgtt tttttcgttt tgacaccggg tttggcggct ggcaggcagc
    10801 ccctccggca agtttccgcc cgaagatgcg ccgccgtgat ggcgtttcag gctgccgccc
    10861 tcctccaact cgcggatgtc gcggcgtatc gtctgcgggg taacgtccaa tgcggcggca
    10921 agctcgtcca ccgacataaa ctgatgccgg cggacaaggc ttaaaatctc tccgtgcctt
    10981 tggattttcg gtttcatcgt tttctgcctc cttgcatcgg gatgccgatt ttaccgcgtt
    11041 cagcccagcg tggaaaacac caccatcaga aacggggcgg caatattgac caccacgccg
    11101 aagctgaccg ctaccggcac gacttccaaa ccgcccgcac cctgaatcac gggcaatgta
    11161 aaatccatac tggtcgcacc gccaaccccc accgccgcat ccggaaaacg cttcatcagc
    11221 agcgggataa atgccagtgc aaacagctct cgtgccaaat cgttcagcag catgatgctg
    11281 ccccataccg cgccgtaagc ctcggtcatg accaaacccg agagggaata ccaaccgaag
    11341 ccggaagcca tcgccaaacc tttcgtccac gacacaccgt ctgccgatgc ggcaaacagc
    11401 agcccgcccg aaagagatga aagcataaac cagaccgaca accgaatacc cctgcggttg
    11461 accaaaacct gccgcaacga taggccgctg cttttgagct gtacgccgat gaggaacacc
    11521 agcagcatca gacaatacat gcccgcgctt tcagacggca tccaaatatc gcgcatcagt
    11581 ttgccggatg caaatccgag cagcacgcat ccgagctgcc ccacactgcc cgacacaccg
    11641 accgaaacgc ccttcccttt cccctttatc cgccacggga ataactttcc caacactgca
    11701 aaagcaagca ggttcgtccc gaccgtacaa acaaacagcc acagaaccgt caacgccata
    11761 tcgtccaacc gcgaacccaa atcctccacg cgcgacaacg atacgccgat cagcagcagc
    11821 acggcataca ccaaaaccga tagcacctta tccaaagcgg gcaggtaagg cttgggcaca
    11881 cggataaaaa atccggcaaa catcggtatc aaaaccgaaa tcaatgtcat caggctgtcc
    11941 atctcctgac ctcctttatt gccgcatgat atgtgcggtt taaaaattgc cgtctgaaaa
    12001 ttgcagatac ccgcatccat atttcagacg gcatcaggtt cgccattaaa aaaccgcctg
    12061 aaggttcagg cggcttatcc gctccggcat tcaatcttcc aaagtctttt ccaaacgctc
    12121 catacagttg cccaaatggc ggcgcaggat tttgaccacg cggttgcgcc tgcccgccag
    12181 cagcaggtcg aggatttcgc ggtgttctga atgcgtatgc gtattgatgg cgtgtttttc
    12241 ctcgcgatgc acgcccgcca cggcgacaat cagggaagac cgcgcgcaca gcgtgttcat
    12301 aatgtcgaac agcacatcgt tgcccaccag gcgcgccagt tcgacgtgga aggcattgga
    12361 caggcggttc cagccaacgc ggtcgcccct gccggaggcc tcttcttcgc gccgtatcat
    12421 cgcataaagc ggcttgaggc gcgtttccaa atccggcaaa tctgcgagga tattcaaaat
    12481 catcgtctcc atttcgatgc gcgcattgaa cacatcctgc atttctttca aatcgggaac
    12541 gtggacgaac gcgcccctgt tgggttgcaa atcgacaatc ttgtcgtgcg ccaaaagcga
    12601 cagcgcgccg cggacggtgt tgcgcgaaca caccatctga cggcaaagtt cggattcggt
    12661 cagctttttg ccgggcagca gcacctgatc ggtaatgccg tccaaaatca gggcgtaaac
    12721 acggaacagc tcagaatcgt gccgctcttc gagaatcagg gaagacgtag tcggcgcatg
    12781 gataatgtcg tcgttttcaa agttcatgat gttttccgta tttttacgct ttcaaatttt
    12841 ttaagatatt ttaaggcggc tgtgtttcaa atcgtgtcag aggaattaaa gcattgcaca
    12901 aatt
//
LOCUS       DAXHRI010000040        12853 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXHRI010000040 DAXHRI010000000
VERSION     DAXHRI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12853
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.040"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..325
                     /locus_tag="ACFM75_000870"
     CDS             <1..325
                     /locus_tag="ACFM75_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HFC6255447.1"
                     /translation="AEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(498..914)
                     /gene="gloA"
                     /locus_tag="ACFM75_000871"
     CDS             complement(498..914)
                     /gene="gloA"
                     /locus_tag="ACFM75_000871"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC6255448.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(939..2108)
                     /gene="lapB"
                     /locus_tag="ACFM75_000872"
     CDS             complement(939..2108)
                     /gene="lapB"
                     /locus_tag="ACFM75_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC6255449.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2119..2433)
                     /locus_tag="ACFM75_000873"
     CDS             complement(2119..2433)
                     /locus_tag="ACFM75_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC6255450.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            2743..3741
                     /gene="ilvE"
                     /locus_tag="ACFM75_000874"
     CDS             2743..3741
                     /gene="ilvE"
                     /locus_tag="ACFM75_000874"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC6255451.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3986..4771)
                     /gene="fabI"
                     /locus_tag="ACFM75_000875"
     CDS             complement(3986..4771)
                     /gene="fabI"
                     /locus_tag="ACFM75_000875"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC6255452.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4926..5747)
                     /gene="dapD"
                     /locus_tag="ACFM75_000876"
     CDS             complement(4926..5747)
                     /gene="dapD"
                     /locus_tag="ACFM75_000876"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC6255453.1"
                     /translation="MSLQNIIETAFENRADITPSTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5757..5987)
                     /locus_tag="ACFM75_000877"
     CDS             complement(5757..5987)
                     /locus_tag="ACFM75_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255454.1"
                     /translation="MPSERRRRGIKVLPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(5957..7540)
                     /gene="pgi"
                     /locus_tag="ACFM75_000878"
     CDS             complement(5957..7540)
                     /gene="pgi"
                     /locus_tag="ACFM75_000878"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6255455.1"
                     /translation="MNAFTRAWHALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSSVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEEGSSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGKQLAKTIIGELEGGTSVHDAS
                     TEGLMAFYRECRLKGGGAA"
     gene            8050..9282
                     /gene="pilG"
                     /locus_tag="ACFM75_000879"
     CDS             8050..9282
                     /gene="pilG"
                     /locus_tag="ACFM75_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC6255456.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9356..10216
                     /locus_tag="ACFM75_000880"
     CDS             9356..10216
                     /locus_tag="ACFM75_000880"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC6255457.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLLTRKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10218..10850
                     /gene="coaE"
                     /locus_tag="ACFM75_000881"
     CDS             10218..10850
                     /gene="coaE"
                     /locus_tag="ACFM75_000881"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC6255458.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAIISH
                     QASESERLLLADDVLLNDGSLKSLCEKTMRLHAFYSGIFASKPTQGKHND"
     gene            10843..11052
                     /locus_tag="ACFM75_000882"
     CDS             10843..11052
                     /locus_tag="ACFM75_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC6255459.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSSQTESLPEISEPDMAYR"
     gene            complement(11137..12813)
                     /gene="pilB"
                     /locus_tag="ACFM75_000883"
     CDS             complement(11137..12813)
                     /gene="pilB"
                     /locus_tag="ACFM75_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC6255460.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         2953 a         3583 c         3356 g         2961 t
ORIGIN      
        1 agcggaaatc actttggaaa cgcctgattc caacacctct gaagcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccagtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa ccggcacagg
      421 ctcaaacagc aggccgacgg caacaaaatg ccgtctgaac cccctaaagg cttcagacgg
      481 cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcgcc gcttttcttt
      541 tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac ggttgtgccg
      601 tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac acgttcgcag
      661 gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc caagtcgtat
      721 cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc atcgccgtaa
      781 ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag cagtttcata
      841 cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg gagcatagta
      901 tggagtaagc gcattttttg tgttcctttc ggtggtggtt aaacttcgat tttattcggg
      961 gtaaacgtct gccatttgtt gcaggcgggg cagtgccaga aaaagacttg ggatttgaag
     1021 tggcagttgc ggcaacggta catcacgctg cgctgtagct gccgtccgat aaccgaacgc
     1081 atcatgtcgg catcggcttt ccaagccgga ttcatatcgc tgagtttcaa accgagcagg
     1141 cggtacacgc cgttaaggtc gggcttgcgg cggacaagct cgacggcggt ttgcgcggct
     1201 tctttctcgc ccttaagcag cagggatttc tcgtacacga cattgatcag gtcaagttcg
     1261 ggaaacgtct gcatatatcc tgtcagacgg ttcaagcctt cttcaggttt tccctgcgcg
     1321 gcataggctt cgtaaagctt ctcgccgacc atgctcaagt atgcatggtt ttgctgctcg
     1381 atggcggcat aggcttcgac ggcggcaggg aaattgcctt gtcggtgttc gatgtcgccc
     1441 aaaatcatgt tggcgcgggt gcattttttg ttggcttcga gtgccttgcc gacattgaaa
     1501 cgcgcgacat cgaaattgga cttgaacagc gcggcttggg caagttcgca ataaaactgg
     1561 gcgatttcaa actgataggt ctgatcgtca tggctgagca gccgggcggt ttcaaccgct
     1621 ttttcccaat ccctgtcctg ttggtagata ttgagcaggt gctgtctggc ttcacgcgcc
     1681 attttaccgt cttgcagccc caaaaaaatc tgttcggcac gatcgaccaa ccccgcactt
     1741 tggtagtttt gcgccaattc aaacaggacg cgcgcgcgct tttcgccgac cgtatcggga
     1801 gaatcgagca ttgtccggtg tatgttgatg gctttgtcgt tttcgccacg ctggcggtaa
     1861 agtttgccga gggtgaggtt caaatcatac gattgcggcc ggccgtcgac gacttccgcc
     1921 aactcccttg ccgcgcgccc gctgttgcgg tcgaccaaag cgtccaagct tttataaaat
     1981 cccgaaggga tgctttttgc ctgtttcaat acggttttca tatccacgcg ggcggcaaac
     2041 cagcccatcg cgaagaagac gggcaaaagg ataatcggca gcaggataat ccacaattcg
     2101 ttgtccatat cggctttctt aaggctgttt ggtagattcg ggcgcatttt gcgccggtgg
     2161 tgcggtcagc tccttccccg tcaaacgcgc atttttcttt acttcggcac gcaacctgcc
     2221 gttctcgcca cgtaacgaca acaaccgtcc gaacaaggca aacattccaa aaataatacc
     2281 gactacaaat gcgccgaaca atacgacaat cagcggcaaa tcgaattttt gccccggcag
     2341 gtaggaaaag gtaacggcat ccgtattaat gacagcaagc agcaggaaga gcagcaggat
     2401 aatgattttg atgacggtat agataagttt catgtttgct ccgtaaataa aaacgccgcc
     2461 cggacagctt cgcactctga accaagattg cgctagttta aacgatttgc acggtttcgg
     2521 ataggatgcg gcagcgtgtt cggacgacat acggagtatg cgattgccga caattttacc
     2581 aaatacaccg ttcctttcca tttgaaaaat aacggattgg acaccgcatc gacagaaaaa
     2641 cccgccgcgc acttgtcaaa accctgtttg caggcgtatc tttacaatct tcaaattcaa
     2701 accgttcatt gaaacatatc agaataagaa aggctttaca tcatgagcag acccgtaccc
     2761 gccgtattcg gcagcgtttt tcacagtcaa atgcccgtcc tcgcctaccg cgaaggcaaa
     2821 tggcagccga ccgaatggca atcttcccaa gacctctccc tcgcaccggg tgcgcacgcc
     2881 ctgcactacg gcagcgaatg tttcgaggga ctgaaagcct tccgtcaggc agacggcaaa
     2941 atcgtactgt tccgtccgac tgccaacatc gcgcgtatgc ggcaaagtgc ggacattttg
     3001 cacctgccgc gccccgaaac ccaagcctat ctcgacgcgc taatcgaatt ggtcaaacgc
     3061 gccgccgatg aaattcccga tgcgcctgcc gccctgtacc tgcgtccgac cttaatcggt
     3121 accgatcccg ttatcggcaa ggccggttct ccttccgaaa ccgccctgct gtatattttg
     3181 gcttcccccg tcggcgacta tttcaaagtc ggatcgcccg tcaaaatttt ggtggaaacc
     3241 gaacacatcc gctgcgcccc gcacatgggc cgcgtcaaat gcggcggcaa ctacgcttcc
     3301 gccatgcact gggtgctgaa ggcgaaagcc gaatacggcg caaaccaagt cctcttctgc
     3361 ccgaacggcg acgtgcagga aaccggcgcg tccaacttta tcctgattaa cggcgatgaa
     3421 atcattacca aaccgctgac cgacgaattt ttgcacggcg taacccgcga ttccgtactg
     3481 acggttgcca aagatttggg ctataccgtc agcgaacgca atttcacggt tgacgaactc
     3541 aaagctgcgg tggaaaacgg tgcggaggcg attctgaccg gtacggcagc cgtcatctcg
     3601 cccgttactt ccttcgtcat cggcggcaaa gaaatcgaag tgaaaagcca agaacgcggc
     3661 tatgccatcc gtaaggcgat taccgacatc cagtatggtt tggcggaaga caaatacggc
     3721 tggctggttg aagtgtgctg atgctttaaa taaaaaaatg ccgtctgaaa cccgtttggc
     3781 gtttcagacg gcattttcgc atccgaaccg tttccgccgc acctgcagca agtcggcaca
     3841 aaggcaatcg gttaaaacaa gcgtccgcat ttcccatccc gcctgccgca agtcgggcat
     3901 ttccctggaa atacgcttca gacggcaaac cgccgcccga aaccgatatg cggcacggac
     3961 ggcgcacgga tttgaaaacg gcagattatc cctcggtgct caaggcattg atgctgtaac
     4021 cgccgtcaac gtaagtgatt tcgccggtaa tgccggacga caggtcggac agcaggaagg
     4081 cggcggtatt gccgacttct tcgatggtaa cgttgcggcg cagcggattg tgggcggcga
     4141 cgtgtcccaa gagtttgccg aaatcggcga tgccggaggc ggcgagcgtt ttgatcggac
     4201 cggcggaaat gccgttgcag cggatgcctt ctttgcccag acaggcggcg gtaaagcgga
     4261 tgcctgcctc aaggctggct tttgccatac ccatcacgtt gtaattcgga atcgcgcgca
     4321 ccgcgcccaa gtagctcagg gcaacgatgg cggagtttct gccgcgcatc atcggacggg
     4381 cggcttttgc caatgcgggc aggctgtatg cggaaatttc gtgtgcggtg ttgaacgctt
     4441 cgcggctgat gctgtcgagg aagtcgccgc tcaaggcttc tttcggtgcg aagccgatgg
     4501 aatgcaccaa accgtccaag ccgtcccaat gtttgcccaa atcggcgaac acttggttga
     4561 tttcgtcgtc gctggcaaca tcgcagcgga atacgagttc ggaatccaat tccgccgcca
     4621 ttttgcggac gcgctcttcc agtttgtcca caacgtaggt aaacgccagt tccgcgcctt
     4681 gttcgcggca ggctttggcg atgccgtaag cgatggaacg ctcggaaatc atgccggtaa
     4741 tcagaatttt tttgccttgc agaaaaccca ttttgtatcc ttcaaatagt gttcggttcg
     4801 ttctaaaccg ttgattatag caaattgtcc ctgtttctgt gttttcacgt tgcagcatgc
     4861 aaacggcaat gccgtccgaa gcggatttca gacggcatcg gacgtttcaa atacggttta
     4921 aggcatcaga tgccgcgcaa caattcgttc acgctggttt tcgcacgggt ttgcgcgtcc
     4981 acgcgtttga cgatgacggc gcagtaaagg ctgtggctgc cgtctttgga aggcatactg
     5041 ccggacacga cgaccgaacc tgccggtacg cggccttggt agatttcgcc ggtggtgcgg
     5101 tcgaagattt tggtggattg accgatgaac acgcccatcg aaatcacgct gccttcttcg
     5161 acaatcacgc cctcaacgat ttcagaacgc gcaccgatga agcagttgtc ttcaataatg
     5221 gtcgggctgg cttgcagggg ttcgagtacg ccaccgatgc cgacaccgcc gctcaagtgg
     5281 acgtttttac cgatttgcgc gcaagagccg acggtcgccc aagtatcgac catcgcgcct
     5341 tcgtcgacgt atgcgccgat gttgacataa gacggcatca gcacgacatt tttcgccaca
     5401 aagctgccgc gtcgggcaac cgcacccgga acggcgcgga agcctgcgtt tttgaactcg
     5461 tcttcagacc agtcggcaaa cttggtcggc actttgtcga agtatttgtt cacgccgtcg
     5521 ttgaggactt cgttgtcttg gatgcggaag gacagcaaca cggctttttt cgcccattcg
     5581 ttgactttcc actcgcccac gcccaagcgt tcggcaacgc gcagtttgcc tgaatcgagt
     5641 tggcggatgg tttccaacac ggcttcttta acttcgggag caacagtgga cggggtgatg
     5701 tccgcgcggt tttcaaaggc ggtttcgata atgttttgca aagacataat gtttccttat
     5761 gtgagatgtt tcgtgggacg cttgccggta ttttcaagca gtatgttcat ttgaaatgaa
     5821 tgcgctattg tgccacgcaa acgcatattc gtcagcaata cgcgggcggt tgccgaacat
     5881 tggcgtttcg gaatcgggcg ggcggcaggt attgcctttt ccgcgcccga atcaatacag
     5941 aaaggcggca gtacttttat gccgcgccgc cgcctttcag acggcattcg cggtaaaacg
     6001 ccatcagccc ttcggtcgag gcatcgtgta cggacgtgcc gccttccagt tcgccgatga
     6061 tggttttcgc caactgtttg ccgtactcaa ccccccactg atcgaagggg ttgacgttcc
     6121 atatcgcgcc ttggacgaag gttttgtgtt cgtaagccgc catcagcata cccagattgt
     6181 agggcgtgag gcgttcgcgt tccgcttcgg gcaaatctgc cagttcggcg cgtgcttcgt
     6241 ccaaggtttt gcccttcatc agggcttcag cctgtgcaaa ggcgttggca acggtaaaac
     6301 ggctgcttcc ttcctccacg ccctgcgccg tcatcgggac gataaagtcg cagggaatca
     6361 ggcgcgtgcc ttgatgaagc agttggaaat aggcgtgctg gcagttgacc ccttcaccgc
     6421 cgaacacgat gccgcccgtt ttgcacacgg cgggactgcc gtctgaagcg cggcttttgc
     6481 ccaaactctc catatcgagc tggttcagcc acgccggcag caggcgcagg ttgtggctgt
     6541 acggaacggc ggtctgcccg tccgcgtgct ggaaattgtt ataccacacg gcaatcagtg
     6601 ccatcagaac gggaatgttg cggcgcggcg gcgtgtggaa aaaatggctg tccatcgcgt
     6661 gcgcccccgc caacaactcg cggaaacgcg ccccgccgac cgcaaccatc acgggcaaac
     6721 cgacggacga ccagacggaa tagcgtccgc ccacccagtc gtacatcgca aacacgcgtt
     6781 ccgccgcgat accgaaagcc gcagctgccg cagtgtcggc agacaccgcg caaaaatggc
     6841 acgccgtttc ggattccgag aaccccgcac cgcgatacca cgccttgact gcctgtgcat
     6901 tgagcagggt ttccggtgtt ttgaaggact tgctggcaac gcaaaacact gtcgtttcgg
     6961 ggttcagacg gcataaaacc gcatccaggc aggcaggatc ggcgttggcg gcaaaatgga
     7021 cggcgatatg ccgtctgaac ggctcaagtg cctgcacgca cattgccggc ccgaggtcgg
     7081 atccgcctat gccgatgtgg acaaaatccg taatccgttt tccggttgtc ccctgatacg
     7141 aaccgtcgtc caaactgtgt gcaaacttca acgcacgatt taactcgcgg cggatttcgg
     7201 gcaacacgtc cctgccgtcc gcataaacgg cacccgcacc gtcgggcagg cgcaaagccg
     7261 tatgcagcgc ggcacgcccc tcactgttgt tgaccttcgc acccgtccgc aaagcacgca
     7321 ttttcccttc caaatccgcc gcatcggcaa gattgcagag cagttgcagc gtatcttcgc
     7381 ccaaacggtt tttgctgtaa tcgaacaaca tcccgtccaa acgctcgtgc atacgctcaa
     7441 aacggtcggg ttcgcaggca aagcggtcgc gcaaaaggac atgacgcgta tcctgataat
     7501 ggcgttcgag cgcatgccat gcacgggtaa aagcattcat ctgttttcct tgatttttca
     7561 gaaccggatt aaaatgtagc agaatgtagt ttaacaaacg gcagcggctt tggcgaatct
     7621 ccggaacacc gcccgccgcg tccaatagca ccttatttcc atatggcgaa aatatagtga
     7681 attaacaaaa atcaggacaa ggcggcgagc cgcaaacagt acaaatagta cggaaccgat
     7741 tcacttggtg cttcagcacc ttagagaatc gttctctctt gagctaaggc gagccaacgc
     7801 cgtaccggtt tttgttaatt cactataccc tccggccggc atttgacccg caacaatatc
     7861 ctgcaagatt tattgtgtac gcataaatgc cggacagccg cctaaataag gtataatcgc
     7921 atccgattct gtcccgtttg ccgcccgcag gcgggatgtc ggcggttttc atcttaccgg
     7981 gaacacgccg gtactgtttt ttaacgatgt ttcttacatt ttattccaat tactttacgg
     8041 ggctagaata tggctaaaaa cggaggattt tctttgttcg caaagaaaga aaaacgcttt
     8101 atctttgaag gcaggcattc cgcctccgac aaactggtca acggcgaagt atccgcgttt
     8161 accgaagaag aggcgcgcaa aaaactggca aaacgcggca tccgcccgtt gcagattacc
     8221 cgtgtgaaaa caagctccaa gcgcaaaatc acacaagaag acatcaccgt tttcacccgc
     8281 cagctgtcca cgatgattaa agcgggcctg ccgctgatgc aggcatttga aatcgtggcg
     8341 cgcggacacg gcaacccgtc tatgacggaa atgctgatgg aaatccgagg cgaagtggaa
     8401 cagggcagct cgttgagccg cgcattctca aaccacccaa aatatttcga ccgcttctac
     8461 tgcaatctag ttgcggcggg cgaaacgggc ggcgtattgg aaagcctgct ggacaaattg
     8521 gcaatttaca aagaaaaaac ccaggccatc cgcaaaaagg taaaaaccgc actgacctat
     8581 ccggtatccg tcatcgccgt cgccatcggt ttggtattcg tgatgatgat tttcgtactg
     8641 cccgccttta aagaagttta cgccaatatg ggcgcggagc ttcccgcact gacccaaaca
     8701 gtgatggata tgtccgactt tttcgtctca tacggctgga tggtgctgat cgcactgggc
     8761 tttgccatat acggcttcct taaattgaag gcgcgttcga ttaaaatcca acggcgtatg
     8821 gatgccatac tgctgcgtat gccgattttc ggagacattg tccgcaaagg gacgattgcc
     8881 cgctggggca ggacgacggc gacgctgatt gcggcaggcg tgcctttggt cgatgtattg
     8941 gactccactg ccggcgcggc gggcaattta atctatgaag aagccacccg ggaaatccgt
     9001 acgcgcgtca tccaaggttt gtctatgact tcggggatgc gtgcgacgga actgttcccc
     9061 aatatgatgt tgcagatgtc ttccatcggc gaggaatcgg gttctttgga cgatatgctc
     9121 aacaaagccg ccgaatttta cgaagacgag gtggacaatg cggtcggcag gctgtccgct
     9181 atgatggagc cgatcattat cgtgatttta ggcttggtca tcggcacgct tctggtcgcc
     9241 atgtatctgc cgctgtttaa tttgggcaac gtggtcgcct gatttgccgc acagatccgg
     9301 cgcggattcg ctctgcgccg gtttgttttt gttttgaata cattgagaac aaaatatgtc
     9361 tgatttgtct gtattgtcgc cgtttgccgt gcctttggca gcggtgttcg ggctgctggt
     9421 cggaagtttc ttaaacgtcg tcatttaccg cgtgccggtc atgatggaac gcggctggac
     9481 ggtatttgcc aaagaatatt taaacctgcc gctgaccgaa gaggaaagcc gtaccttcaa
     9541 cctgatgaag ccggattcct gctgtcccaa atgtcgcgtg ccgatacgcg cgtggcagaa
     9601 catcccgatt gtcagctacc tgctcctgcg cggcaaatgc gcttcctgcc aaaccaaaat
     9661 cagcatacgt tatcccttaa tcgagctgct gaccggcgta ttgttcgggc tggttgcctg
     9721 gcaatacggc tggtcttgga ttacattggg cggtttgata ctgaccgcgt ttctgatttc
     9781 cctgaccttt atcgatgcgg acacccaata cctgcccgac tcgatgacct tacccttgat
     9841 ttggctgggg ctgatattta atttggacgg cggcttcgtg cctttgcagt ctgccgtttt
     9901 aggtgcggtt gccggctatg gttcgatatg gctcttatgt gcagtgtata aactgctcac
     9961 aagaaaaacc ggtatgggca acggagattt caaactgatt gccgcattgg gcgcgtggct
    10021 cggcatatcc gcattgcccg tgctgatttt tgtttcctcg ctgatcggtt tggtcgcggc
    10081 aatcgttatg cgcgtcgcca aggggcagca ttttgccttc ggccccgcac tgacagtttc
    10141 gggctggata atttttacgg caaacgattc cgtatggcgg gcggtcaact ggtggctgac
    10201 ccatccggtg ctgtaagatg acggtatggg tcggactgac cggcggaatc ggcagcggca
    10261 aatcggcagc cgcgcaatgt tttgccgatt tgggcgtgcc gcgcatcgat gcagacgcgg
    10321 cggcgcactc gctgacggct tcagacggca tcgccctgcc ggaaatcagg cggctgttcg
    10381 gcgacaccgt tttcgacaca cagggtttgt tgcggcgcga catattgcgt aaagaagtct
    10441 ttgcctcccc atcgcgaaaa gccttgctcg aatccgtgat gttgccgctg attttctcag
    10501 aaatcaaaaa acagcaagaa acctttaccg atgcggttta cggcattgtc gaaattccgc
    10561 tgctgacgga aaagcgtcaa tttatcagcc tgatacggcg tgtcctgacc ataagtgccc
    10621 ctgtggaaaa acgtatcggc agggtgatgg cccgcagcgg gctgacgcgc ggcgaggtgg
    10681 ctgccatcat cagccatcag gcatccgaat ccgaacgcct gctgcttgca gacgatgtgc
    10741 tgctcaatga cggcagcctc aaaagcctgt gtgagaaaac aatgcgcctg cacgcgtttt
    10801 attcagggat tttcgcctca aaaccaacac aaggaaaaca caatgactga atcgcggcaa
    10861 acacgccttc aagtcaaatg tccgacctgt caaacggcag tagtatggaa accggaaaac
    10921 gcattccgcc ccttctgttc gcaacgctgc aaactgatcg acttgggcgg atgggcagac
    10981 gggaaatata cggtttccag ccaaacggaa agtttgccgg aaatatccga acccgacatg
    11041 gcataccgct gaccgccccg ccttcccggc aaacaccctg aaagtcaaat gccgtctgaa
    11101 acaaacacgc ttcagacggc attttcattc tcaaacctaa tcgttggtat ttgccgttac
    11161 ctcttccaat gaagtaatgc cctgcataac tttcaaaata ccggcccggc gcaaatccac
    11221 cataccctcc ttataggcaa cgtccaaaat atccacttcc gtaccgttgt tcataatcac
    11281 acgctgcatt tcttcgctga tgggcataac ctcatacacg cccgcacgcc ccttataacc
    11341 ctgcccccgg caacggtcgc aaccgacggc gcggtaaagt ttccaatctt ttgcaaggtc
    11401 ctcatcggtg aagccgactt ccttcaaagc agaggcagac gggcgttcca cttcctgttt
    11461 gcagctcgaa cacagcctgc gtaaaagacg ctgcgccata atcaggctga ccgaactggc
    11521 aatattaaac ggcgcgacac ccatattcag catacgcgac aacgtcgccg gcgcattatt
    11581 ggtgtgcagg gtggaaaaca ccatatgccc tgtttgtgcc gccttaatcg caatatcggc
    11641 agtttccaaa tcacgaatct caccgaccat aatgatgtcc gggtcctgac gcaggaaaga
    11701 cttcaaagca gcggcaaaag tcaggccctg cttatcattg acgttaacct gattgatgcc
    11761 cggcaggtta atctcggcag ggtcttccgc cgttgcaata tttaccgact ccgtattcaa
    11821 aatattcaaa caggtataga gcgacaccgt cttacccgaa cccgtcggac cggttaccag
    11881 caccatcccg tagggacggt gaatcgcttc caacaacaat tttttctgaa acggctcaaa
    11941 accgagctgg tcaatgttca aagacgcggc atcggaattc aaaatccgca tcacgacctt
    12001 ttcgccaaac agcgtcggca atgtgctgac acggaaatcg acaggcttgc cgcccttttg
    12061 aaaggtcagc tgcatcctgc cgtcctgcgg tatccgtttt tcggaaatgt ccaaacgcga
    12121 cattacctta atccgggaag caagctgccc ccttaccgca atgggcggct gaaccacctc
    12181 gcggagctgc ccgtccacac ggaaacggat acgggcattg tgttcgtaaa actcgaaatg
    12241 gatgtccgat gccccgctgc gcaaggcatc cgacaaagtc ttatggataa acctcggaac
    12301 agggccgtct tctgcctcct cgttgtcgat atacagggtg tggctttcct cttcctcctg
    12361 cccctcccca agctcctgaa gcagcgatgt cgaacgcgaa cccacccaat cgagcaaacc
    12421 cgccaactgg tcatcctcga caatgaccaa ctcaacctca atccctgcgg cagaaacggt
    12481 tttctgaatt tgcggcatct gtgtcggatc ggaaaccgca aaaaatactt tgtcgccccg
    12541 acggaaaacc ggcacacagt ggaactccac catctgctcc tccgtcaaca cccccatcag
    12601 caccctgtgg cgcggataat gacgcaaatc aagaatcgaa taactgaaca ccctcgcaat
    12661 caatgccgca agcgacttgg gcgaaatgac accgtctgaa aacagcatcg gcaacacttc
    12721 cttacccgcc tgcgactcat tgtagtaatg ctcggcctgc tcaacagtaa ccacctggtt
    12781 ttgaaccaga atcctcagca aacctacgct catacgacct tatccccaaa tttattcatt
    12841 gttatacctg tac
//
LOCUS       DAXHRI010000041        12798 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXHRI010000041 DAXHRI010000000
VERSION     DAXHRI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.041"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..215
                     /locus_tag="ACFM75_000884"
     CDS             <1..215
                     /locus_tag="ACFM75_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255461.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQGN
                     KPLGAEELADLKPLYKDLKNNIGNIV"
     gene            546..971
                     /locus_tag="ACFM75_000885"
     CDS             546..971
                     /locus_tag="ACFM75_000885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255462.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQISEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTSTALPRLSSKRTIL"
     gene            complement(1113..3413)
                     /gene="recQ"
                     /locus_tag="ACFM75_000886"
     CDS             complement(1113..3413)
                     /gene="recQ"
                     /locus_tag="ACFM75_000886"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC6255463.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGRSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQCRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMAGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKIDRFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
     gene            complement(3478..4260)
                     /gene="trpC"
                     /locus_tag="ACFM75_000887"
     CDS             complement(3478..4260)
                     /gene="trpC"
                     /locus_tag="ACFM75_000887"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC6255464.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAENAPPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIETEVGKLF"
     gene            complement(4310..5266)
                     /locus_tag="ACFM75_000888"
     CDS             complement(4310..5266)
                     /locus_tag="ACFM75_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HFC6255465.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARTVPGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRRTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRTDEVSEAV
                     TLFQPAHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNSR"
     gene            complement(5263..5394)
                     /locus_tag="ACFM75_000889"
     CDS             complement(5263..5394)
                     /locus_tag="ACFM75_000889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255466.1"
                     /translation="MPISFFGKRFSTPIFGRASPTSAPAGRAAASSMPSENKKTDTL"
     gene            complement(5398..6936)
                     /gene="murJ"
                     /locus_tag="ACFM75_000890"
     CDS             complement(5398..6936)
                     /gene="murJ"
                     /locus_tag="ACFM75_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC6255467.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            complement(6936..7127)
                     /locus_tag="ACFM75_000891"
     CDS             complement(6936..7127)
                     /locus_tag="ACFM75_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255468.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGRFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            7154..7852
                     /locus_tag="ACFM75_000892"
     CDS             7154..7852
                     /locus_tag="ACFM75_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6255469.1"
                     /translation="MKSRQLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(7909..8913)
                     /gene="amgK"
                     /locus_tag="ACFM75_000893"
     CDS             complement(7909..8913)
                     /gene="amgK"
                     /locus_tag="ACFM75_000893"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC6255470.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            8983..11391
                     /locus_tag="ACFM75_000894"
     CDS             8983..11391
                     /locus_tag="ACFM75_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC6255471.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            11496..12620
                     /locus_tag="ACFM75_000895"
     CDS             11496..12620
                     /locus_tag="ACFM75_000895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6255472.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKAAKA
                     AKAAKAAKVAKVAKVAKVAKVAKVAKVAKVAKVAATAQKEAAPAQQQGGIRFSDGIAA
                     VADNEVITRRRLAEAVAEAKANLPKEAQISESELSRQVLMQLVNQSLIVQAGKRRNIQ
                     ASEAEIDAVVAKNSALKNLSPAQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQ
                     AQKQGITLPEGAPLRQYRAQHILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQ
                     YSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDA
                     GTPQERIRNSVRQYIFQQKAEQATVNLLRDLHSGAYVDIR"
BASE COUNT         3012 a         3582 c         3541 g         2663 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacaca tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg aggagctggc ggatttgaaa ccgctttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgccgtc gtgccgacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atatccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcta aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaaatgg cataaaaatc
      901 tatatgatag tggattaaca aaaaccagta cggcgttgcc tcgccttagc tcaaagagaa
      961 cgattctcta aggtgctgaa gcaccaagta aatcggttcc gtactatccg tactgtctgc
     1021 ggctcgccgc cttgtcctga tttttgttaa tccactataa aaacaggaat ttttaaatag
     1081 aggcaatgcc gtctgaaact tggtaacggg cttcagacgg catttcgttc caataccgcc
     1141 aacaccgccg caccgtaacg tgcggctttt tcttcgccta cgccgtatac ggcggcaagc
     1201 tccgccaaac cttccggctg tttggcggca atggcgcgta gggcggcttt gctgagaatg
     1261 cggtagggtt cggactgttc gtgttttgcc gtttcgttgc accattggat cagggcgcgc
     1321 atcaggcggc gtttgtgttt ggcggtttca tcgatgccgt ctgaaaacgg acggcagacg
     1381 gcgaggatgt cccgtccgta tttggcggcg cgtacgctgc ccaagccgtg tacgccttcg
     1441 aggtcggttt cggtttcggg cgtatcggca agcatatcgg caaggctttc gtcggagagg
     1501 acggcatgca gggcgcagtt ttccgccctt gcctgttcat accgccaggc ctcgagtttt
     1561 tgacgcagtt gttgttcgcg ttcggtttgc ggacggatga ccgcgtcgcg gctgaagccg
     1621 gcggcgttgc ggcagacttc gaggatgccg tgtccgaaac ggtcgatttt ggcttcgccc
     1681 aaaccgtaga tgtcgtgcag tccgttgagg ttttgcggca ttttttcgac aaggtcgcgc
     1741 agggttttgt cgccgaaaat catataggcg gggatgcctt cggcttctgc ctgtttcata
     1801 cgccaaacgc gcaatgcctg ccacaggcgt tcttcgcgtt cggtacgcag ccagttgtct
     1861 ttgagggtgc gggcggcggg cttgtcgcgc ttgagcggac gcagcatcac ttcggtttcg
     1921 cctttgagga cttttttggc ggcttcggtc agttgcaatg cctgatatcg ggtaatgttg
     1981 acggtgaggt agccgaggct gatacactgg cggatgacgc tgcgccattc tttgtcggac
     2041 aactccgtac cgatgccgaa ggtggacagt tgttcgtgcc ggttgccgcg tatccaatcg
     2101 tcgcttttgc cgcgcaaaag gttggtgatg taaccggcgg caaaacgttg tccggcgcgg
     2161 tacacgcagc tgagtaattt ttgcaccaac accgtgccgt caaaccgtac gggcggatgc
     2221 aggcagttgt cgcaatggcc gcagggttcg gattcttcgc cgaaatgttt gagcagcagt
     2281 acgcggcggc aggcggcggt ttcgcagacg gaaagcatgg catcgagttt ttgcatttcg
     2341 atttgctttt gcacctcgtc gctgttgcct tcggcaatcc gttcgcgcag caacacccaa
     2401 tcgttcaaac cgtaacacag ccagctcgcg gcaggcagcc cgtcccggcc ggcacgcccc
     2461 gattcctgat agaaatgttc gacactctgg ggcatatcga gatgggcgac aaagcgcacg
     2521 tcgggtttgt ctatgcccat gccgaacgcc acggtcgcca ccacgataat attgtcttca
     2581 tgcgtaaagc ggcgttggtt ttcctcgcgc acgtccatgc tcaaaccggc atgatacgga
     2641 atcgcgttta atccgttttc acgcaaaaac cgcgccacat cttccacctt tttgcggctt
     2701 aaacaataca caatgccgct ttgtcccgcc atttctttgc ggataaattc aagcagttgt
     2761 tttttgccgt tatttttttc gataacctga taataaatat tcgggcggtc aaagctggag
     2821 acaaattcgg gcgcatcgtc caagtgcaga taatgcttga tgtcggcgcg tgtggccgca
     2881 tcggcggtgg cggtcagggc gatgcgcggg acgttcggat agcgttcggc aagcatgccg
     2941 agctgttggt attcggggcg gaaatcgtgt ccccactggc tgacgcagtg cgcttcgtca
     3001 atggcgaaca ggctgacggt ttgttggtcg agaaaacgca aaaagcggtc ggtaaccaag
     3061 cgttccggtg cgacataaag cagtttcaga cggcattggg caagcttgtc ggcaatctcg
     3121 cgcgcctcat ctgccgatgt gccgctgttg actgccgctg cttcaatccc ggccacatgc
     3181 aggctggcca cttggtcgtt catcagcgca atcagcggcg atacgacaac cgccacgcct
     3241 tcgcgcatca gcgcgggaat ctggtaacac aaagacttgc ccccgcccgt cggcatcagc
     3301 accgtcaaac tcctgccgcc tgccaaagta ttgatgacag cctcctgcct gccgcgaaat
     3361 tcgggataac cgaatacttc gtgcaaaatc tgtttggcgg tcggtcggtg catgatggtt
     3421 ccgtgcttgg taagggtgtt ggtcggtcgg cggcaatatg ccgtctgaaa tcgggattta
     3481 gaagagtttg cccacttccg tctcaatatc gtcggcacgc atgaacgttt cgccgatcag
     3541 gaaggtatgc acgccgcgcg cccgcataaa ttccacatcc gccttacccg taatgccgct
     3601 ttcggtaacg acggttttgc cttccagcgc gggcagcagc gacagggttt ggtcgaggga
     3661 gacttcaaaa gtccgcaggt tgcggttgtt tacgccccac aacggcgtgg tcagattgcg
     3721 gcatttttcc aattcggttt catcgtgcag ctcgagcagg acggtcatgc ccaattcgtg
     3781 cgccaccgct tcaaagcgtt ccaattgttc ctgttccagt gctgcggcaa tcagcaggac
     3841 ggcatccgcc ccccatgcgc gcgcctgata aacctggtat tcgtcgatga tgaagtcttt
     3901 gcgcagcacg ggcagcgata cggcttcgcg cgcttgtttg aggtattcgg gcgaaccttg
     3961 gaaatagggt tcgtcggtca gtacggacaa acacgccgct ccggcgtttt cataggcgcg
     4021 tgcaatctcg gccggtcgga agtccggacg gatcaggcct ttgctcgggc ttgccttttt
     4081 gatttcggca atgacagcgg gcaggtttag acggtgtttg ccgcgtatcg aatcgatgaa
     4141 gctgcggacg ggcggtgcgt tttcggcaag tgcgcggatg tgttcggcgt tgacggcggc
     4201 tttttgggcg gcaacttcct gtgctttggt ggcaaggatt ttattgagga tgtcggtcat
     4261 gtcgggctcc gtattcgtct gggaaagggg gaatattagc atcaaaccgt taacgactgt
     4321 ttgtgcggaa gctgtcgaaa taggacagga cggtctgcgg cagccattgc aggtgcagcc
     4381 tgccaccggt gctgccgaca aagccgacat gaccaccgtg tgccggctgg aacagggtaa
     4441 cggcttcgga cacttcgtct gtgcggggca gggcttcggg cggcaggaag gggtcgttgg
     4501 cggcgttgag caggagcagc ggtttggcaa cgtgtttgag cagcggtttg caggaagttc
     4561 ggcggtagta gtcgtgccgg tcggcaaagc cgtgcagcgg tgcggtgaaa cggtcgtcaa
     4621 actcgcccag tgttttgcac cctgcggcaa atgccgtctg aaaacccgga actgtccgtg
     4681 ctttgggtat cagggtgcgg aggaagtagc gcgtgtagag cagccgcgtg atgccgctgt
     4741 cgaagcgtct gcctgccgcc tctgcatcga cgggggcgga gatgacggca gcggcttgcg
     4801 gcagcgcgtt ttcgccctgt tcgcccaaat attttgccag cgcgttgccg cccagtgata
     4861 cgccgacggc gtatatttca cggtaacgcg cggcaagcgt gtccaaagta aaggcgattt
     4921 cggcggtatc gcccaagtgg tagaacaccg gagcggtgtt ggcaatgccg ccgcagctgc
     4981 ggaaatggac gactgcgccg tgccaaccgc ggctgcgtac cgcgagcatc agttcgaccg
     5041 cgtaatggct gcggctgctt ccttccaaac cgtgaaacag cacgaccagc ggcgcatcgg
     5101 gcgaaatgcc gtctgaaaaa tcgtaggcga ctttggtttt acccgtgctg tcgggaagca
     5161 gctcgcggcg gtatgtgggt gcggggcgtt gcaggaattt ggcggcaatc gtgtcggcat
     5221 tgccgttgcg gaggaaaaag ggcgtgtccg gcggtgttaa aatcataagg tatcggtttt
     5281 cttgttttca gacggcattg atgatgcggc agcccgtccg gctggtgcgg acgtggggga
     5341 tgcgcgcccg aatataggcg tggaaaagcg tttgccgaaa aaggatatcg gcatcggtca
     5401 gttttccacg cgtttgaaat ggcgcggacg gaagcccaaa gccgccagtg atgcgaaata
     5461 cagtccgccg ccgacggcaa tcaggatgca gagctgcccc gctttccgca ttccgccggc
     5521 gcgtacccat tcaaacggca ggcaagcctg cgccgcccac agtccgccgc ccatcacggc
     5581 gagcgagagc agcattttcg ccaagaacgc cgcccaaccc ttgccaggtt ggtaaatacc
     5641 gtgtctgcgc aacaggtaaa acaacaatcc ggcattgata cacgcgccca gaccgatggc
     5701 aagcgaaagt ccgacgtgtt tcagtgggcc gataaaggca aggttcatca actgcgtgca
     5761 aatgagcgtg aagatggcga ttttgacggg cgttttgatg ttttgccgcg cataaaagcc
     5821 gggcgccaac actttaatca tgattaaacc gattaaaccg aaagaatagg caatcagcgc
     5881 gtgttgcgtc atctgcgcgt caaacagcgt gaattctcgg tacataaaca aggttgccac
     5941 cagcgggaac gacaacaccg ccagtccgac cgccgccggc agcgtcagca gcatgcacag
     6001 gcgcaaaccc cagtcgagca gggcggaaaa ctgttccgta tcttggtttg ccgagtgttt
     6061 ggacaaagtc ggcagcaaaa tcgtaccgag tgccgccccc agcacgccgc cgggcagctc
     6121 catcatgcgg tcggcgtaat acatccatga aacgctgccc gattgcagat aagacgcgaa
     6181 aatcgtgttg atcaccaaag aaatctgcgc cacgctcacg cccaaaatcg caggcgccat
     6241 ctgtttcatg acgcggttga ccgccgcatc tttgaaattc agtttgggca gtttcaaaaa
     6301 acccagtttc gccagccagg gcagttggaa gccgagttgt aaaatgccgc cgacaaaaac
     6361 cgcccaagcc agcgcggtaa cgggaggatc gaaatacggc acgaaaaaca gcgcgaatac
     6421 gataaacgac acgttcagga acgtgggcgt aaacgccgga atgctgaatt tatgatagga
     6481 attgagtacc gagccgacaa aagaggaaag tgaaatcaat aagatatagg ggaacgtaat
     6541 ccgcagcaaa tcgatggaga gctgaaattt gtcggcatct ttggcaaaac cgggtgcgga
     6601 aacataaatc acccaaggcg cggcaagtat gcccagcgcg gtaacgatga ccagcacaaa
     6661 cgacagcatc cccgccacat ggcggataaa agcctccgtc gcctctttcg aacgcgtttc
     6721 cttatattcc gccaaaatcg gcacaaacgc ttgggcaaac gccccctccg caaacacgcg
     6781 gcgaagcagg ttgggcagtt tgaacgcgac aaaaaacgca tccgtcgcca tgcccgcgcc
     6841 gaatgcgcgc gcaatgaccg tatcgcgcac aaatcccaaa acgcgcgaca ccatcgtcag
     6901 gctgccgact tttgccaaag ctcccagcat attcatcatt gttcctcaac agtcgtaccc
     6961 gtttggggca acggcgcgta ttgtacgaca gaaaccgctt cagacggcat cgggtttgat
     7021 gccgtctgaa gcggtttccc gaaacgaaaa cgtccttttc cggcggcaaa ctgtatcaac
     7081 acgcggaaat gcaataaaat agccggattc cgattgattt ccaacatctg tttccaacat
     7141 cacggagagc cgtatgaaat ccagacaact cgccctcggc gttgccgccc tgttcgccct
     7201 tgccgcgtgc gacagcaaag tccaaaccag cgtccccgcc gacagcgcgc ctgccgcttc
     7261 ggcagccgcc gccccggcag ggctggtcga agggcaaaac tataccgtcc ttgccaaccc
     7321 gattccccaa cagcaggcag gcaaagtcga agtccttgag tttttcggct atttctgtcc
     7381 gcactgcgcc cacctcgaac ctgttttgag caaacacgcc aagtctttta aagacgatat
     7441 gtacctgcgt accgagcatg tcgtttggca aaaagaaatg ctgccgctgg cacgcctcgc
     7501 cgccgccgtc gatatggctg ccgccgacag caaagatgtg gcgaacagcc atattttcga
     7561 tgcgatggtc aaccaaaaaa tcaagctgca agagccggaa gtcctcaaaa aatggctggg
     7621 cgaacaaacc gcctttgacg gcaaaaaagt ccttgccgcc tacgagtccc ccgaaagcca
     7681 ggcgcgcgcc gacaaaatgc aggagctgac cgaaaccttc caaatcgacg gtacgcccac
     7741 ggttatcgtc ggcggtaaat ataaagttga atttgccgac tgggagtccg gtatgaacac
     7801 catcgacctt ttggcggaca aagtacgcga agaacaaaaa gccgcgcagt aagcccgttt
     7861 gaaaaatgcc gtctgaaact tggttttcag acggcatttt gattgggttt aaaacgtaaa
     7921 gcccgtttcc agttcttcat cgccgaccag ttcgaccaag agcgcgtaga gcggggcgag
     7981 ttcggcataa cggcgcgata cgcggcgcag gtaattgagg aaacgcggga tttcgggacg
     8041 gtatttgtct ttgccgtcgc ggtagtacag gcgtgcgaag atgcctgcaa ccttcaagtg
     8101 ccgctgcacg cccatccatt cgaaccagcg gtaaaactcg tcaaacgctt cggggacggg
     8161 caggccggca gcccgcgcct tttcccagta gcggataacc aagtccaaga caaattcttc
     8221 ttcccattcg ataaaggcat cgcgcaacag cgacaccaaa tcgtaggaaa tcgggccgta
     8281 aagcgcgtct tggaagtcta aaacgcccgg cctgccgcgc gtcagcatca ggttgcggac
     8341 gataaagtcg cggtgcacat agactttggg ctgcgccaac aggggcggca gcagcgtatc
     8401 gacggtttgc tgccaaagtt ggcgttgttt gaatgttaat tcgcgcccca attcttttgc
     8461 gacaaaccat tccgggaaca ggttgatttc gcgcaacatc gtttcacggt catattcggg
     8521 caaaacccct tcacggctcg ccttctgcaa ttcgaccaac tcgccgattg cctccaaaag
     8581 cagggctttg tgcgccgttt cgccctgctc ctgaagcatc gcggtcaaaa acgtcgtatt
     8641 gcccaagtcg ttcaatacca caaaccccaa atccgtgtcc gcgtgcaata cctgcggtac
     8701 attgaccatg tcaaacagtt tctgcacttt caaataaggt gcgacactca tcttgtcggg
     8761 cggtgcatcc atgcagacga cactgctgcc gtctgaaaac gttgcacgga aatagcggcg
     8821 gaaatcagca tccgccgccg cgaaggacag atcgaagtcc cgttcgggat agatggtctg
     8881 aagccaattt ttcagttcgg tttgccgttg cataacagta ctaaagcatt tcaggttaca
     8941 ataaacgcta ttctaactgg caaaccgact tgaggggcga ttttggctcg tttattttca
     9001 ctcaaaccac tggtgctggc attgggcttc tgtttcggca cgcattgcgc cgccgccgat
     9061 gccgttgcgg cggaggaaac ggacaatccg accgccggag gaagcgttcg gagcgtgtcc
     9121 gaacccatac agcctaccag cctgagcctc ggttcgacct gcctgttttg cagtaacgaa
     9181 agcggcagcc ccgagagaac cgaagccgcc gtccgaggca gcggcgaagc atccatcccc
     9241 gaagactata cgcgcattgt tgccgacaag gtggaaggac agtcgcaggt gcaggtgcgt
     9301 gccgaaggca acgtcgtcgt cgaacgcaac cggacgaccc tcaataccga ttgggcggat
     9361 tacgaccagt cgggcgacac cgttaccgca ggcgaccggt tcgccctcca acaggacggt
     9421 acgctgattc ggggcgaaac cctgacctac aatctcgagc agcagaccgg ggaagcgcac
     9481 aacgtccgta tggaaaccga acacggcgga cggcggctgc aaagcgtcag ccgcaccgcc
     9541 gaaatgttgg gcgaagggca ttacaaactg acggaaaccc aattcaacac ctgttccgcc
     9601 ggcgatgccg gctggtatgt caaggccgct tccgtcgaag ccgatcggga aaaaggcata
     9661 ggcgttgcca aacacgccgc cttcgtgttc ggcggcgttc ccattttcta caccccttgg
     9721 gcggacttcc cgcttgacgg caaccgcaaa agcggcctgc ttgttccctc actgtccgcc
     9781 ggttcggacg gcgtttccct ttccgttccc tattatttca accttgcccc caatctcgat
     9841 gccacgttcg cgcccagcgt gatcggcgaa cgcggcgcgg tctttgacgg gcaggtacgc
     9901 tacctgcggc cggattatgc cggccagtcc gacctgacct ggctgccgca cgacaagaaa
     9961 agcggcagga ataaccgcta tcaggcgaaa tggcagcatc ggcacgacat ttccgacacg
    10021 cttcaggcgg gtgtcgattt caaccaagtc tccgacagcg gctactaccg cgacttttac
    10081 ggcaacaaag aaatcgccgg caacgtcaac ctcaaccgcc gtgtatggct ggattatggc
    10141 ggcagggcgg cgggcggcag cctgaatgcc ggcctttcgg ttctgaaata ccagacgctg
    10201 gcaaaccaaa gcggctacaa agacaaaccg tatgccctga tgccgcgcct ttccgccgat
    10261 tggcgcaaaa acaccggcag ggcgcaaatc ggcgtgtccg cacaatttac ccgcttcagc
    10321 cacgacagcc gccaagacgg cagccgcctc gtcgtctatc ccgacatcaa atgggatttc
    10381 agcaacagct ggggttacgt ccgtcccaaa ctcggactgc acgccaccta ttacagcctc
    10441 aaccgcttcg gcagccaaga agcccgacgc gtcagccgca ctctgcccat tgtcaacatc
    10501 gacagcggcg caacttttga gcggaatacg cggatgttcg gcggcggagt cctgcaaacc
    10561 ctcgagccgc gcctgttcta caactatatt cctgccaaat cccaaaacga cctgcccaat
    10621 ttcgattcgt cggaaagcag cttcggctac gggcagctct ttcgcgaaaa cctctattac
    10681 ggcaacgaca ggattaacac cgcaaacagc ctttccgccg ccgtgcaaag ccgtattttg
    10741 gacggcgcga cgggggaaga gcgtttccgc gccggcatcg gtcagaaatt ctatttcaag
    10801 gatgatgcgg tgatgcttga cggcagcgtc ggcaaaaaac cgcgcaaccg ttccgactgg
    10861 gtggcatttg cctccggcag catcggcagc cgcttcatcc tcgacagcag catccactac
    10921 aaccaaaacg acaaacgcgc cgagaactac gccgtcggtg caagctaccg tcccgcacag
    10981 ggcaaagtgc tgaacgcccg ctacaaatac gggcgcaacg aaaaaatcta cctgaagtcc
    11041 gacggttcct atttttacga caaactcagc cagctcgacc tgtccgcaca atggccgctg
    11101 acgcgcaacc tgtcggccgt cgtccgttac aactacggtt ttgaagccaa aaaaccgata
    11161 gaggtgctgg cgggtgcgga atacaaaagc agttgcggct gctggggcgc gggcgtgtac
    11221 gcccaacgct acgttaccgg cgaaaacacc tacaaaaacg ctgtcttttt ctcacttcag
    11281 ttgaaagacc tcagcagtgt cggcagaaac cccgcagaca ggatggatgt cgccgttccc
    11341 ggctatatcc ccgctcactc tctttccgcc ggacgcaaca aacgaccctg accgtcggaa
    11401 acccggcagg agcaccgttc ccgaacaaga cggcattcca ccgacaaccc caaacccgcc
    11461 atcaaaggta ggattcaaac gataaggaaa gaatgatgaa aatcaaagcc ctgatgattg
    11521 ccgccgcatt gctggcagca gccgatgtcc acgccgcacc gcaaaaggca aaaaccgcat
    11581 ccgccaaagc tgccaaagct gccaaagctg ccaaagctgc caaagctgcc aaagctgcca
    11641 aagctgccaa agttgccaaa gttgccaaag ttgccaaagt tgccaaagtt gccaaagttg
    11701 ccaaagttgc caaagttgcc aaagttgccg ccacggcgca aaaagaagcc gcacccgcac
    11761 aacagcaggg cggtatccgc ttttcagacg gcattgccgc cgttgccgac aacgaagtca
    11821 tcacgcgccg ccggcttgcc gaagccgttg ccgaagccaa agccaacctg cccaaagagg
    11881 cgcagataag cgaatccgag ctgtcccgac aggtgctgat gcagcttgtc aaccaatccc
    11941 tgattgtaca ggcgggcaaa cgccgcaaca ttcaagcaag cgaagcggaa atcgatgccg
    12001 tcgtcgcaaa aaattccgcc ctcaaaaacc tcagccccgc ccaacgccgc gattttgccg
    12061 acaacatcat tgccgaaaaa gtccgccagc aggcagtgat gcagaacagc cgcgtgagcg
    12121 aagctgaaat cgatgccttc ctcgagcagg cgcaaaaaca aggcatcacc ctgcccgaag
    12181 gcgcaccgtt gcgccaatac cgcgcccaac acatcctgat taaagccgac agcgaaaacg
    12241 ccgccgtcgg cgcggaaagc accatccgca aaatctacgg agaggcccgc agcggcacag
    12301 acttttccag cctagcgcgc caatattcgc aagacgcgag cgcgggcaac ggcggagatt
    12361 tgggctggtt tgccgacggc gtgatggttc ccgcctttga agaagccgtc cacgcgctca
    12421 aacccggaca ggtcggcgcg cccgtccgca cccaattcgg ctggcatatc atcaaattga
    12481 acgaagtgcg cgatgccggc acacctcagg aacgtatccg caattccgtg cggcaataca
    12541 tcttccaaca aaaagccgaa caggcaaccg tcaacctgtt gcgtgacctg cattccggcg
    12601 cgtatgtcga catccgctaa ggcggtttga agcaaaaagc cataccgatc ggcaaaaatc
    12661 cgggcggtat ggcttttttg aatttcgagt tacttttaca ccgtcattcg tcattcccgc
    12721 gtaggcggga atctagcaac gaaaagtaac aggaatttat cgggaaaaca gaaacctcta
    12781 tgccgtcatt cccgcgaa
//
LOCUS       DAXHRI010000042        12515 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXHRI010000042 DAXHRI010000000
VERSION     DAXHRI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.042"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(484..1776)
                     /locus_tag="ACFM75_000896"
     CDS             complement(484..1776)
                     /locus_tag="ACFM75_000896"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC6255473.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1854..2822
                     /locus_tag="ACFM75_000897"
     CDS             1854..2822
                     /locus_tag="ACFM75_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255474.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(3023..4489)
                     /locus_tag="ACFM75_000898"
     CDS             complement(3023..4489)
                     /locus_tag="ACFM75_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6255475.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEAKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4731..6863)
                     /gene="autA"
                     /locus_tag="ACFM75_000899"
     CDS             complement(4731..6863)
                     /gene="autA"
                     /locus_tag="ACFM75_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoaggregation autotransporter AutA"
                     /protein_id="HFC6255476.1"
                     /translation="MTLKASKQASKQASKQASKQASKQASKQASGRVNLLTLSVLSLF
                     CTPYAWGSDAYDPVKEAEIKNKFILEAAENRDSHVWRGPCSISFDCFGMFSAQLGSNT
                     RSTEIGGDADFSFSDKPANGVSHYFSSGKTDPNSSEALYDEINITGKNYNSGILAVDN
                     MPVVKKYVTENYGADLKQAVKDQLKDLYKTRPEDWEKNKKIVEEAIIKQYGIPRNKIN
                     KFRKVIDPIIEEAVLTPHSNTSQTSLNNIFNKKLHVKIENKSHVAGQVLELTKMTLKD
                     SLWEPRRHSDIHMLETSDNARIRLNTKDEKLTVHKAYQGGADFLFGYDVRESDKPALT
                     FEEKVSGQSGVVLERRPENLKTLDGRKLIAAEKADSNSFAFKQNYRQGLYELLLKQCE
                     GGFCLGVQRLAIPEAEAVLYAQQAYAANTLFGLRAADRGDDVYAADPSRQKLWLRFIG
                     GRSHQNIRGGAAADGRRKGVQIGGEVFVRQNEGSRLAIGVMGGRAGQHASVNGKGGAA
                     GSYLHGYGGGVYAAWHQLRDKQTGAYLDGWLQYQRFKHRINDENRAERYKTKGWTASV
                     EGGYNTLVAEGVVGKGNNVRFYLQPQAQFTYLGVNGGFTDSEGTAVGLLGSGQWQSRA
                     GIRAKTRFALRNGVNLQPFAAFNVLHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGW
                     KGHMSARIGYGKRTDGDKEAALSLKWLF"
     gene            complement(8262..8507)
                     /locus_tag="ACFM75_000900"
     CDS             complement(8262..8507)
                     /locus_tag="ACFM75_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255477.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            8619..9047
                     /locus_tag="ACFM75_000901"
     CDS             8619..9047
                     /locus_tag="ACFM75_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC6255478.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            9059..9535
                     /locus_tag="ACFM75_000902"
     CDS             9059..9535
                     /locus_tag="ACFM75_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC6255479.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWNGSLFEVD
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSKHPFSSWKNAV"
     gene            complement(9886..10374)
                     /locus_tag="ACFM75_000903"
     CDS             complement(9886..10374)
                     /locus_tag="ACFM75_000903"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC6255480.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEAALALCASAEETVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(10442..11497)
                     /gene="aroG"
                     /locus_tag="ACFM75_000904"
     CDS             complement(10442..11497)
                     /gene="aroG"
                     /locus_tag="ACFM75_000904"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC6255481.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            12164..12421
                     /locus_tag="ACFM75_000905"
     CDS             12164..12421
                     /locus_tag="ACFM75_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255482.1"
                     /translation="MTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLEFDPYYRH
                     KTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK"
     gene            complement(12403..>12515)
                     /locus_tag="ACFM75_000906"
     CDS             complement(12403..>12515)
                     /locus_tag="ACFM75_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC6255483.1"
                     /translation="LNPIEKVWANIKRYLQTVLSDYARFDDALLSYFDFN"
BASE COUNT         2869 a         3425 c         3075 g         3146 t
ORIGIN      
        1 ggtttaaatt taatccacta tatttgcaaa cactgccgga acggataaat ccggattcct
       61 acccacttcg tcattcccgc gaaagcggga atccggtgcg ttgggtttca gtcatttccg
      121 ataaactcct accgcattgt atttctagat tcccgctttc gcgggaatga cggcggatgg
      181 gtttccgtgc cgtggtgtcc ggattcccga cggtacggga atgacggcag tgcttcctta
      241 acttagtttt attttaatcg actgtatggt ggattaacaa aaatcaggac aaagcgacga
      301 agccgcagac agtacaaata gtacgaaacc gattcacttg gtgcttcagc atcttagaga
      361 atcgttctct ttgagctaag gcgaggcaac gacgtactgg tttttgttaa tccactataa
      421 atgccgtctg aaaacctgtt tgcaggtttt cagacggcat tgttttcgtc gccggttcaa
      481 acttcaatcc gattgcgaca cggtaaccgg tatgccgcgc aagcgcgcaa gcagcgcgtc
      541 cgctttctgt ttctgcgcgg caaacgccgc cttgtccgcc tgcgtcaatt cgggtgtcgg
      601 caatgcgacc gaaacaggat taacgggctg cccattgatg cgcgcctcgt aatgcaggtg
      661 cggccccgtc gaacgcccgg tcgaaccgac aaaaccgatg acctcgccgc cgcgcacatt
      721 gccttgtgcc tgcgaaaacg cgctcaagtg cgcgtacagc gtttccacac cgttggcgtg
      781 gcgtatcatc accgcgttgc cgtagccgcc cttccggcct ttaaaggtaa tcacgccgtc
      841 ggcggaagcc ctgaccggcg ttccctgcgg tgcggcataa tcgatgcccg tgtgcagccg
      901 ccaagtgtgc aggatggggt gcatacggta gccgaacggc gaagaaatgc gcgtatagac
      961 cagcggctcg atgttgaagc cgcctttttc ctgcaacacc ctgccgtctt catcataata
     1021 attgccgccc cctccgcctt ccttgtccga acggtaatag aacgcctgat gccttgtgcc
     1081 gcccttaacg acttcagccg ccaaaatatc gcccgccgcc acctgctgcc cgtggaaata
     1141 caggctgtcg taaatcaggc gcacggcatc gccttccttc aaaccgtcaa ggctgaagcg
     1201 gccggcgaaa atcccgctta aggattcgcg gatttcgacg ggcacttccg cccgcgccag
     1261 cgaaccgcgc gccgacgttt tgaccacgac cgaacgcagc gtcggcaaaa ccttcatatc
     1321 cgcctcagaa gccgaccgcc gccatatgcc gcctttcttt tccaaagcga ccagattgcg
     1381 ctcgccgtct tcgtcggtaa aaaactgcac ttcgcgcgcg ccgccgtcgc cgccgaccaa
     1441 aacatgaacc gactggtcgg cacgcaaatg ccgcaaatcg gcttcgccgc catatttttc
     1501 cgttattcgg gcaatttcgt cccgcgccat acccgaacgc gccagcacgt ccgccagcga
     1561 gtcgcctggc tgcaccgcct cctgcaccca atatgccgtc tgaacaccgc tgccgcccca
     1621 agacagcggc ggcagttttt gttccacgcg ctgcggtctg acgcgctccg tcctctctgt
     1681 cgaagcaatg tatgccgccg acaccaaaat aatcgaaacg gcaagcgcgc gcagcgcgta
     1741 tttccgatgt tttgccgaaa gtgggaagac agccatattg ttcctttttg acggcgtttg
     1801 cgccgaatcc tctataatgt tgcgcctgac ctgcatttct gaaacgtcaa acgatgaaac
     1861 tcaccctcgc cattccctcg ctcaatttgg atgaagacga aacccgaata cctctctgcc
     1921 tgcccgcctt caacaaaatc ctgcaatacg gctcgctgca ccgccaaagc tgcacggctt
     1981 cggcatttta cgcgcgttat ttgtggtgcg gcagactggc ggaacgcgcg gcacaaagcc
     2041 tgaatatgcc gtctgaagcc gtcgcgctcg ccacgcccgt ctggcaaaag atggggatgc
     2101 atcaggcaaa tgtgctaacg gcggaatatc tgaacgtcgg tacggatgaa gcggaacggc
     2161 tctgccgcga cctgtcggcg ttttacggcg acattccatg gcgtttcgtt cccgtcctgc
     2221 ccgaattatg gctggtttcc cttccttgcg cgtaccgctg gggcgcgaag cccgttttgg
     2281 acttgggcgg attgttgggc gcggacgatc agccggacgg ggaagacgcg ttggaatggc
     2341 tgcgcgtcca aaccgaaatc caaatgtggc tggcggcgca ccccgtcaac cataaccgca
     2401 agaaacgcgg gctgcccgag ttgaacgggc tgtggctttg ggacagtctg cacggaagca
     2461 gtcaaggcgg cacgcttttc gccgatacgg tttggagccg tttccacccc gaccgccgcg
     2521 cattgcccga caacttccgc gcctatgaag aaacggcggc atacctaccc gacacgcacc
     2581 atatcctgtt tatggacgat ttgcgcctga ccgccctgac aggggacagg gagcggtatg
     2641 cggcaatatt gcagcagtgg gaagaacgct ggttcgcacc gctttacgaa gccgtccgca
     2701 tcggcaaaat caaacggctg gacatcgcca cggacggaca acacggcggt acgctgacct
     2761 tcaaacccgc cgacaggtgg aaattttggc gcggcgcgaa aacctttgac ggaatctggt
     2821 gaacccgccc gccgcaaccg cgacaatgcc gtctgaagcc cgaatcgggt ttcagacggc
     2881 attttacctc gcatcgccgt tatgccggaa aaaccggaat gcgtttccgc acacgcggcg
     2941 gcacgcaaag caccgcccga aagccgccga tgcccgccgt gataccgccg ccggcagggt
     3001 ttcctccgaa aaggaacagc aatcagaacg ttttattaaa ctcgacaaac gcccgatttt
     3061 tctcgtattc gttgaacaca tcgttactcc gcgtttcgcg gtgcgacaac gtcaggcgcg
     3121 gcgtgatgcc tttgaaatgc aatgcccggt gccaaaggct caaggatgtg ttcaattctt
     3181 tatccctgcg cctttcccct ttaaaaccgc tgaaaaagcc gggtttttca taatgccgtt
     3241 tcgccgcgcc gaggcgcaac agcgaagaca ggccgctgcc gccccattcc tgcccccagg
     3301 caaagcgcag gccgtaacgg ttgaaattgt cgccccggtc ggcggggttg cgctcgcggt
     3361 aaaaatccaa accgcccatc caatattggc gcgcattccg gtaaaacacc agcgaattgg
     3421 aaatttgcaa atgggtattg tcggaacgcg cccggcgcgt attcttcaaa cgcccccact
     3481 ccgccgaaga caacgtttgc catttcgggg tttgccaacg gttgaaataa aggcgtgcgc
     3541 cgttggtgta agaataagcg tcgttgccgt aggtgcggcg ttcgtggaac actgccagcc
     3601 cggcatcttt gcgccggtcg gcaaaaccga tgccgccgga aacgcctgcc gtcatatcgt
     3661 tgaatttctt attccccgga taaaccctgc cggacacgtc gccgccagcc gtcgtgtacc
     3721 agccgttttt cagcgaccat tttttctccg cgccgagccg gtaattgacc gccgtgccgt
     3781 ccacctgttt cgggaaagtc catttgccgt actgctgccg tttcggggct tggttgatat
     3841 tgtgttcgcg ggtgacgctg aagccgccat ttaccttcca cgcatcgcgt tcgcgcaatg
     3901 ccttgcggta cagctcgacc tgctccatca gctgcggcgg caggttttcc gccttcaggc
     3961 ggtcgaactg gtctgccgcc gcctcgttct gcctgttttc aaacaatgcc gccgccaaac
     4021 gcatacggac ggcgggcgcg tcgggttggg cggcaatcaa ttcccggtaa tgggaaatcg
     4081 cctccttcac cctaccgtct gcctgcgcca aaatcccttg tgcataaagt gccaacatct
     4141 tatcctgctg cgcctgttgt aggtaaattg gcaaaataac gcggataccg gcaatattgt
     4201 ttgaaaccac tgcggaatac atcgcgcggg acagtaattc gggatttttc agcagggttt
     4261 cgccgtcaat ctgcaaaact tttccttttt cccgcacctg ccccggtacc ttctccctgt
     4321 cgatcggttt ggcctccgct tcatgaagcc tgaactcggg acggcttctc aaatccggtt
     4381 cgcgcggtgt ttcttcggca tatgccgcgc ttgccaataa aggcagcagc agcatccatc
     4441 tgtttcgtgc aggcataaat gtcttcccac aaaaataaaa aataaccatt atcaaccggc
     4501 ggattgtatc agttttttat gccgctgttc caaaccctgc cgcccgatgc cgcgacaatg
     4561 ccgtctgaag ccagaatcgg gtttcaaacg gcggcaaaaa accggcggtt ttccgccggt
     4621 ttcatcatta tgccccgccc ttcggggcgg cagggttcgg attggtttgc ggggcggcgc
     4681 gcggtgccgt gccggtgtgc cgcctgtacg tcaaaacatt tcccggcgca tcaaaacagc
     4741 catttgagcg acaatgcggc ttctttgtcg ccgtccgtcc ttttgccgta tccgatgcgt
     4801 gcggacatat ggcctttcca accggcttca atgccgaacc gcccttcgag tgccgtcctg
     4861 cctgccagcg tctgtttttc gccgtccatt tccacgccga aagattttga cctgtgcaaa
     4921 acattaaaag cggcaaaagg ctgaagattg acaccgttac gcaaagcaaa acgggttttt
     4981 gcccgaatgc cggcgcggct ttgccactga ccgctgccga gcagtccgac cgccgtcccc
     5041 tcgctgtcgg taaagccgcc gtttacgccc aagtaggtaa actgcgcctg cggttgcagg
     5101 taaaaccgca cattattgcc ttttccgaca acgccttccg ccacaagcgt gttgtagccg
     5161 ccttcgacag aagccgtcca acctttggtt ttgtagcgtt ccgcacggtt ttcatcattg
     5221 atgcggtgtt tgaaacgttg gtattgcaac cagccgtcca aatacgcacc cgtttgttta
     5281 tcgcgcaact gatgccacgc agcataaaca cccccgccat aaccatgcaa ataactgcct
     5341 gccgcaccgc ctttgccgtt gactgatgcg tgctggccgg ccctgccgcc catcacgccg
     5401 attgccagcc ggctgccttc attttgccgt acaaacacct cgccgccgat ttgcacgcct
     5461 ttgcgccgcc cgtccgcagc cgcgccgccc cgtatatttt gatgcgaccg gccgccgatg
     5521 aagcgcagcc acaatttttg acgggacgga tcggcggcat acacgtcgtc tcccctgtcg
     5581 gcggcacgca gcccaaacaa agtatttgcc gcataagcct gttgggcata taaaaccgct
     5641 tccgcctcgg ggatagccaa acgctgcacg cccaagcaaa atccgccttc gcattgcttg
     5701 agcaataatt cgtacagtcc ctgccggtaa ttttgtttaa acgcaaacga attagagtct
     5761 gccttttccg ccgcaatcag tttgcgcccg tcgagcgttt tcagattttc cggccggcgt
     5821 tccaaaacca cgccggattg tccgctgact ttttcttcaa aggtcagggc gggtttgtcc
     5881 gactcccgca cgtcgtagcc gaacaggaaa tccgcaccgc cctgatacgc tttatggacg
     5941 gtcagttttt catctttcgt gttcaggcgg atgcgggcat tatcggaagt ttccagcata
     6001 tggatgtcgg aatggcggcg cggttcccaa agggaatctt tcagcgtcat cttggtcagt
     6061 tccaacacct gtccggcgac gtgggatttg ttttcgattt tgacgtgtaa ttttttattg
     6121 aagatgttgt tgagactagt ctgtgatgta ttactatgag gagtgagtac agcttcttct
     6181 ataattggat caataacttt tctaaattta ttaattttat ttctaggtat tccatattgt
     6241 tttattatcg cttcctcaac tattttttta tttttttccc aatcttcggg tcttgtttta
     6301 tataaatcct ttaattgatc cttaaccgcc tgctttaaat cagctccgta attctctgta
     6361 acatatttct taacaacggg catattatcg acggcgagga tgccgctatt gtagttttta
     6421 cccgtgatat tgatttcgtc atacaaggct tcagatgaat ttggatcggt tttaccgctg
     6481 gaaaaataat gggaaacgcc gtttgccggc ttgtctgaaa atgaaaaatc ggcatcgccg
     6541 ccgatttcgg tagaacgagt atttgaacca agctgagcac tgaacatacc aaagcaatca
     6601 aaagatatgc tgcacgggcc gcgccaaacg tgtgaatctc tgttttccgc cgcttctaaa
     6661 ataaatttgt ttttaatctc ggcttctttg acgggatcgt acgcatccga accccaagca
     6721 tatggcgtgc aaaacagcga taaaacagat aatgttaata gattaacccg accgcttgct
     6781 tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct
     6841 tgcttgcttg ctttcagtgt catgagaaat tttcctttgt caagtgttaa aattataatg
     6901 attatatact acatcacact acatcgcaat gaaaaatcag gaaaaacaaa aaacctctcc
     6961 gccgtcattc ccgcgcaggc ggaaatctag aattttaatg cggcaagaat ttatcggaaa
     7021 aaactgaagt ttaaagacct agattcccgc ctgcgcggga atgacgggat ttcaggttac
     7081 tgtttttgat tttctgtttt gtggaaataa cgggattttg gatgatgtgc atttatcgag
     7141 aaaactaaaa accctccgcc gtcattcccg cgaaagcggg aatctagacc tttcggtgcg
     7201 gaaacttatc ggataaaacg gttttccctg catcctggat tcccactgtc gtgcgaatga
     7261 cgggatatag gtttcactac ggacgtgttc agattaccgc ctgcgcggga aggacgggat
     7321 ttcaggttgc tgtttttgat tttctgtttt tgtgggaata acgggacttt ggatgatgtg
     7381 catttatcgg gaaaacaaaa aaccctccgc cgtcattccc gcgaaagcgg gaatctagaa
     7441 ttttaatgct gcaagaattt atcggggatg accgaggttt gaaggtctgg attcccgcct
     7501 gcgcgggaat gacggtttag aaatcacctg aaacgagaaa aagaaccgaa accggacaag
     7561 tcggattccc gcacgggcgg gaaggacgga atttcagatt gttgtttttg attttctgtt
     7621 ttgtgggaat aacgggattt tggatgatgt gcatttatcg ggaaaactaa aaaccctccg
     7681 ccgtcattcc cgtgaaagcg ggaatctaga atttcaatgc cgcaagaatt tatcggggat
     7741 gtccggggtt taaaggtctg gattcccgcc tgcacgggaa tgacggttta gaaatcacct
     7801 gaaacgagaa aaagaaccga aaccagataa gtcggattcc cgcacgggag ggaaggacgg
     7861 gatttcaggt tactgttttt gattttctgt tttgtgggaa taacgggatt ttggatgatg
     7921 tgcattttcg ggaaaactaa aaaccctccg ccgtcattcc cgcgaaagcg ggaatctaga
     7981 attttaatgc ggcaaaaatt tatcggaaaa aaccgaagtt taaagaccta gattcccgcc
     8041 tgcgcgggaa tgacggttta gaaatcaccc gaaacgagaa aaagaaccga aaccgaacaa
     8101 gccggattac cgcctgcgcg ggaatgacgg ctcaaaagtt acgaaacgaa aaataaccga
     8161 aaccaaacaa gtcggattcc cgcgcgggcg ggaatgacga taagcgtaat ttcaatctgc
     8221 catattgtct tttcacttgc ctgaaaacag atttcagcca cctatccctg catccggcgg
     8281 ctgatgtagt cggcaatcaa atcccgcttt tcccaaaaaa acttctgcat tttttgttcg
     8341 gaatacagtt tgcggaccag agtgggcaaa ccgctgtgga cgatggcggc gatttctttg
     8401 ttgccttttt cttcctgcat tgccgtctga aaatccatac ccctgccttt cagatatgct
     8461 gcaaggcggt tgcggatgtg ggaattgagt tcgatggatt tgagcatttt gcctccgttt
     8521 tgcggtaagg atttattcga tttgtttatc cgcctgattt tatcacgttt cccaagcggt
     8581 ttgcctgtac aataccgctt ttcggcaggg tgtgggcgat gatgttttct tggttcaagc
     8641 tgtttcactt gttttttgtc atttcgtggt ttgcagggct gttttacctg ccgaggattt
     8701 tcgtcaatat ggcgatgatt gatgtgccgc gcggcaatcc cgagtatgtg cgtctgtcgg
     8761 gcatggcggt gcggctgtac cgttttatgt cgccgttggg cttcggcgcg gtcgtgttcg
     8821 gcgcggcgat accgtttgcc gccggctggt ggggcagcgg ctgggtacac gtcaaactgt
     8881 gtttgggctt gatgctcttg gcttaccagt tgtattgcgg cgtgctgctg cgccgttttc
     8941 aggattacag caatgctttt tcacaccgct ggtaccgcgt gttcaacgaa atccccgtgc
     9001 tgctgatggt tgccgcgctg tatctggtcg tgttcaaacc gttttgacgg agcatcttat
     9061 gccgatagaa atcgaacgcc gttttttgat tggaaacgac aactggcggc aatacgccga
     9121 cgaaccgctg ctgttgaggc agggttatct gtctgttgaa aaagaacgca ccgtccgtgt
     9181 ccgcattgcc ggacaacggg cgtggctgac gctgaaaggc tatatttcgg aaatcagccg
     9241 cagtgagttt gaatacgaaa tcccgcttgc cgatgcggaa aagatgatgg aaacgatgtg
     9301 cccgtttaaa atggaaaaat ggcgttatcc ggtcagatgg aacggcagcc tgtttgaagt
     9361 cgatgttttc cttggagaaa acgcaccttt ggtcgtcgca gaaatcgagc tgcccgacga
     9421 aaatgccgat tttgaccgtc cggactggct ggggcgcgaa atcactgccg acggtatgtt
     9481 taccaatgct tatttgagca aacatccgtt ctcaagctgg aaaaatgccg tctgaagcgg
     9541 cttcagacgg cattctccga caaccatcgc aaatccaaag acagcctcta tatccttatt
     9601 tctgctttct ttgttccatt attcccaata aatccccata acctaaaatc tcgtcattcc
     9661 cgctcaggcg ggaatccggt tctttaaact tcggtttttt ccgataaatt gctttagctt
     9721 ttcgtttccg gattcccgct tttgcgggaa tgacgatgga aagattgttg ttatttcgga
     9781 taaattaccc taactttaaa atcccggatt cccatcttcg ggggaataac aatattttct
     9841 atttcagccc atcatttgcg ggggatttta tagagagttt atatttcaat atctcaaata
     9901 atgcacaaaa gcatagctcg tgcctttgct gcttacacgg cgttccgtcc gctctgcttc
     9961 tttccaatgc gtccggtcta tttctgggaa aaatgcatct ccttccacag acaaatccac
    10021 ttcggttatc cgcaaatcgg tcgccaatgg catcgcttgt ccgtatatct gcgcgccgcc
    10081 cataatgacg gtttcttccg cgctggcgca caatgccaat gccgcctcca aacttgccac
    10141 agtttccgcg cctgccgcgc aataatccgc ctgccggctg atgacgatgt tcctccgtcc
    10201 gggcaggggt ttgacgggca gggattccca cgttttccgc cccataatga cgggtttgcc
    10261 caaggtatag gctttgaaaa atgcgaaatc ttcggggatg tgccaaggca tagcattgcc
    10321 cgccccgatg cacaggtttt ccgcacacgc cgcaattagg gttattttga gcatatttcc
    10381 tccacaaaaa tgccgtctga acgcttcaga cggcatttta tgacattccg tgtcaaaaat
    10441 ctcaaccggc gcgcgccata cgttttttgt ttgcacctgc caacaatgcc aacagttctt
    10501 cagtcgcgcc ccaaccgata cacgcatcgg taatgctttt gccgtacact tccggcttat
    10561 cctgtctgcc ttcgaccaaa tggctttcca ccatcacgcc cataatattg ctcccgtcct
    10621 gttccaattg ggcagcaata tcttgtgcca cttccatctg ccgggtgtaa tccttgcggc
    10681 tgttggcgtg gctgcaatcg atcatcagct tatcggttac ccctgccgca cgcagttgtt
    10741 ccgccgcctc gctgacgtgt cccgcatcat aattcggctc ttttccgccg cgcaaaatga
    10801 catgacagtc gggattgccg ccggtatgga caatggcgga atgtccggcc ttggttacag
    10861 acaagaaatg atgcgaatgg ctcgccgcac cgattgcgtc gatggcaatc ttcaaattgc
    10921 catccgtacc gtttttaaag ccgacggggc aggacagccc gcttgccaat tcgcggtgaa
    10981 cttggctttc ggtcgtccgc gcgccgattg ccccccaaga aatcaagtcc gcataatatt
    11041 gcggcgtaat catatccaaa aactcggtag aggcaggcat acccatattg ttcagcgaca
    11101 acaacaggct gcgtgcctga cgcaaaccga aattgatgtc aaacgtaccg tccaaatgcg
    11161 ggtcgttaat caaacctttc caacccaccg tcgtcctcgg cttctcgaaa taaacgcgca
    11221 tcacaatcaa aagctcgttt tcatactgct tgcggagttt caacaaacgc tccgcatatt
    11281 ccaacgccgc tttcggatcg tgaatcgaac acggcccgat aataaccaac agccgcttgt
    11341 ccctgccgtg aaccaaatcg gaaatttcct gacgggtgcg gtgaaccaag cccgaagcct
    11401 ctttggaaat cggcagctcg taaagatggg caatcggcgg caacaactct ttaacttctt
    11461 taatcttaat atcgtctgtg gggtaatggt gtgtcatctt gcatctttcc tgatgggttt
    11521 ttatacgcgg cttaggttac atcgtttacc ttgcacaaac aagtgcaaca tattactaca
    11581 aagctatttt atttcaaaat aactgatata tcttttttca tagattttaa atttcatatt
    11641 tttaatattt taggaatttt attccaaaga aaaagaaaca gattgataaa aacaataccc
    11701 gccattcatt cgggcaaaac cgcacggaac atccgttttc ccatttccac gacaatccgg
    11761 tacagaacac cccgctgccg attccgcctg cacatccggc aaaatttcat acgcaaggaa
    11821 aatgttgaaa acaaaaagaa ctcattgctt gaaaaaagat ttaatgggaa tgcgggtatt
    11881 tcccacactt tttccattta tttatcctca ttataggaag aaaaacatga aaaaaccctg
    11941 cttgcactct cacttgcagc cgtgtccgcc gttacggtag ccgatattcc cgagtctaac
    12001 ggcaaaggca aagccatcat cggcgataag gaaaaacacc gttaccaagc atgaaaacct
    12061 tgctttgaaa gtccatggcg aaatccgcca agatttgggg gacggatgat ccctaacccc
    12121 ttgggcggat gcatatgcag atttgcgcgg caaaaccaaa gtgatgacga cccagatgtg
    12181 tgcttcccgc gatgtcagca aaagcgccaa aggttggagt gtcggtatcg gtctgaatgt
    12241 aggcaaacag ttgaccgaca gcgtcggtct cgagtttgat ccatactacc gtcacaaaac
    12301 aatctgcaaa ccccgtgaga ttgttttgga cggcgacaaa accaaaatgg gccgctccaa
    12361 atccaacgag tacggcttcc gcgtaaccgc aacgttctat agtcaattaa aatcaaagta
    12421 ggacagtagt gcatcgtcaa atcgggcgta atcagacaaa acggtttgca gataccgctt
    12481 aatattcgcc cacactttct caatcgggtt gagtt
//
LOCUS       DAXHRI010000043        12254 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXHRI010000043 DAXHRI010000000
VERSION     DAXHRI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.043"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(107..319)
                     /locus_tag="ACFM75_000907"
     CDS             complement(107..319)
                     /locus_tag="ACFM75_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255484.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGALQGRITFLNHPF"
     gene            complement(316..1371)
                     /locus_tag="ACFM75_000908"
     CDS             complement(316..1371)
                     /locus_tag="ACFM75_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6255485.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1477..1959)
                     /locus_tag="ACFM75_000909"
     CDS             complement(1477..1959)
                     /locus_tag="ACFM75_000909"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6255486.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(2006..3142)
                     /locus_tag="ACFM75_000910"
     CDS             complement(2006..3142)
                     /locus_tag="ACFM75_000910"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC6255487.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKSWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            complement(3139..3684)
                     /locus_tag="ACFM75_000911"
     CDS             complement(3139..3684)
                     /locus_tag="ACFM75_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFC6255488.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3681..5156)
                     /gene="trpE"
                     /locus_tag="ACFM75_000912"
     CDS             complement(3681..5156)
                     /gene="trpE"
                     /locus_tag="ACFM75_000912"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC6255489.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(5267..5632)
                     /locus_tag="ACFM75_000913"
     CDS             complement(5267..5632)
                     /locus_tag="ACFM75_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255490.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(5653..7563)
                     /locus_tag="ACFM75_000914"
     CDS             complement(5653..7563)
                     /locus_tag="ACFM75_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6255491.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLAIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERLNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7582..8226)
                     /locus_tag="ACFM75_000915"
     CDS             complement(7582..8226)
                     /locus_tag="ACFM75_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6255492.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8318..9568)
                     /gene="glyA"
                     /locus_tag="ACFM75_000916"
     CDS             complement(8318..9568)
                     /gene="glyA"
                     /locus_tag="ACFM75_000916"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC6255493.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLENVRKQITALCDKYPVYGA"
     gene            complement(9723..9986)
                     /locus_tag="ACFM75_000917"
     CDS             complement(9723..9986)
                     /locus_tag="ACFM75_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255494.1"
                     /translation="MSNQTKIQAIKQASEQIFAICETPNTALQAIHLILRHGGAGELS
                     WQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDALPLGSLVLT"
     gene            complement(10049..11824)
                     /gene="ggt"
                     /locus_tag="ACFM75_000918"
     CDS             complement(10049..11824)
                     /gene="ggt"
                     /locus_tag="ACFM75_000918"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC6255495.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(11992..12254)
                     /locus_tag="ACFM75_000919"
                     /pseudo
     CDS             complement(11992..12254)
                     /locus_tag="ACFM75_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
BASE COUNT         2743 a         2798 c         3352 g         3361 t
ORIGIN      
        1 gcgagaacgc tgtactggtt tttgttaatc cactataaat gttccgatac gaactgcaaa
       61 atattggttt tgtttctgac aggcaaaagc actgtttatt tggctgtcaa aagggatggt
      121 taaggaaagt tatgcgcccc tgaagcgcgc cccagataag gatggttgcg ccgacggctt
      181 cagacggcat tttggcggcg gtgttgggtt ttgtatccgg tttgccgttg tgtcttgtga
      241 tgatgatttt gggcgcggtt tttctgtttt gatgtgtgga atgccgtctg aaaggcggtt
      301 cagacggcat agcggtcatt tttgtgcggt caggcggtcg aatatgccgc cgtcggcgaa
      361 gtaggttttc atgatgttgt cccatccgcc gaatttttct tcgggagaga atgagtctaa
      421 gtctgggaag tcggctttgt gtcttgccaa tacttcgggg ttgcgggggc gcaggtagag
      481 tgaggcggcg agttcttgcg ccggttcgct ccaaaggtat tcgagatagg cgcgggcggt
      541 tttttgcgtg ccttttttcg cgacgacgct gttgacgacg gcgacggggc tttcggcgga
      601 aatggtgtag ctcggataga cgatttcaaa ctgtccttgg gtcagttttt tgctgacgta
      661 gttggcttcg ttttcaaaag taatgagtac gtcgccgatg ttgcgttgtg tgaaggtggt
      721 ggtggcggcg cgtccgccgt tttcaaaaac gggggtgttt ttgaggatgg atgcgacgag
      781 tttttgggct tcctgttcgt tgccgttggt ggttttcaga ccgtaaccgt atgcgccgag
      841 gaaggcgtag cgtccgttgc ccgaggtttt gggattggcg atgacgatgt taacgccgtc
      901 tttggcaagg tcgttccaat cgcggatctg tttggggttg ttttttcgga caaggaaaac
      961 catagtgctg gtgtagggcg cagcgtggtc ggggagggct tgttgccagc ctttttctac
     1021 cagtcctttt ttttcgagca ggtcgatgtc ggaggattgg ttcatggtta cgacatcggc
     1081 ttgaaggccg ttggctacgg ataatgcctg tttgctggag ccgccgtggg actgttggat
     1141 gctgacggat gtgccggggt gttcggattg gtatgttttg ataaataagg ggttgtattc
     1201 tttgtaaaaa tcccgtgcca catcgtatga ggcgttgagc agggtaatgt tttttccgtc
     1261 ggattcggta ttggccgggg cattttgtcc ggacggatgg tttgaatcgg ctgcggggct
     1321 gcaggcggtg agcagggctg cggtatagag tgccggtgcg taggttttca tatgcttgtc
     1381 ctgtcggttg gtagatgggg caactttata cggctgtctg cgcttgtgga aataatgttt
     1441 gatttgaaga ttatcagttt tggttataag gacggatcag aggtgtttcc gcatcagttc
     1501 gcatttgatt ttgatgctgg ggtcaagctg caatactgcc gaaccgagcg attcgtagcg
     1561 gttgagaagg taaaacggga cggagttgag cgatgcgtag agtttcagaa agctcaaacc
     1621 ggatttgtgg gcgatggttt ccgcctgatg aagcagggca gtgcccagtc cgaggttgtg
     1681 gaacaggggg tggacgtaaa gtgcatcgag ttgtgcttct tggcagtcga tttggaaaaa
     1741 tccctgtatg ttgcctttgt attcggcaac ccaaagtgct ttgtcgggat cggaaatggt
     1801 cggcaggtag ctttctgtgt ttaacaagcc ttcccatact tttagggcgt gttcgttgta
     1861 gctgaggatg caggtgtatt ggacggagtg caggtggact ttgaagatgt ctttgcagtc
     1921 ttgcacggtg gcggggcgta acagtgtcag caggttcatg gcagtatgtc ggcggcttca
     1981 gacggcatct gtgccgttgg tcggattata gggactgatg cagttttttt gcttcttgaa
     2041 atgcggtgtc cgaatcggtg gttaaaacgg taaagtgtcc cattttccgc cctttgtgcg
     2101 cggttttttt gccgtaaagg tgcaggtgcg cgtccggccg gctttgcagg ggaaaccaat
     2161 ccggttcgcc gccgtcttcc tgccaaacgt cgcccaaaat attcgccata cagcaagaac
     2221 tcagcaattt ggtgtcagca ggtggcaggt tgcacatcag gcggacctgt tgctggaatt
     2281 ggtctgccgc gcaggcgtcg acggtatggt ggccggaatt gtgcggacgc ggcgcgattt
     2341 cgttgacgac caattcatgc gtgtcgccga caacaaacat ttctaccgcc aatacgccga
     2401 cgtagttcaa ttcatcggcc aaacgctgcg ccatttgtcg cgcctgttgc tgaatgtcgg
     2461 cactcagtcg ggctgggacg atggagtagg cgaggatacc gttttcgtga atgttttcgg
     2521 caggatcgaa agtttgcacg ttgtcattgt tcagacggca tacgataacg gaaatttcgc
     2581 cgcgcaagtc caccattttt tccaaaacgc aatccacgcc gcggtgttcg gcaaacgcgg
     2641 ctttgagttc atccaccgtt ttgacgcgga tttggccttt gccgtcatag cccaatgtag
     2701 cggttttcag gatgccgggc agaaattgta tgctttcttc agtgatgtct tcggctttgc
     2761 aaattgcttg atacggcgcg gtttgcaggc ctgctttgcg tatccatgac ttttcctgaa
     2821 tgcggttttg cgcgatggca acgcagtcgc cgctggggga aacattggta tgtttggcga
     2881 gaaaacgcat cgcatcggca ttgacgtttt cgaactcggt cgtaacagcc gcacattttg
     2941 ccaattcttc caaagcggtt tggttgtcaa acggcgcaca caaatggcgg tcggcaaatt
     3001 ccgctgccgg cgcgtccgga tcggggtcga gaacggtgac tttgtagccc atggttttgg
     3061 cagcaacagt aaacattctg cctaattgtc cgccgccaag aatgccgagc atggcgggcg
     3121 gagaaagaga tatgtttttc atgctgactc ttcaaattgt acaagttgat agctataact
     3181 aattcttgac ggatgtcttg tatcgctgga attaccagtt tcagaaatac agaatacttt
     3241 ttccataaat ttttctgctt ttagaaattc cagtattctg tttttttcat ccttataagc
     3301 actgcggtct gtaccccatg caagaataat catatcagca tcttccaaac atcctttaaa
     3361 tttggaaaaa tcggtttggg tgtttgccct aattcctgtt tgctgtgtag agtaactgga
     3421 aaaaatattt aacattttga agttggtaaa accgtacata tccaagaaac gtgcaagttg
     3481 ggtaagggtt ttgtcgcttc tttcatcatt tgctttactt ggattaattc ctatagcaac
     3541 agctgagaaa tttttaggat tttctgtgtt gccgctccat ctaaccgtta ggatttctcg
     3601 acttttttca ttatctgtat agagaccgtc tttggtagtc ggtctgagtg tttggcgaag
     3661 ctcataaagt ttttcataag tcatttatcc aacccttcct gcaccatctg cgcggcgcgt
     3721 ataacggctc gggctttgtt ttgtgtttcc tgccattcgg aggccggatc ggagtcggca
     3781 accacgcccg cgccgctttg gacgtatagc gtgttgtttt ttactacggc ggtgcggatg
     3841 gcgattgcca aatccatgtc gttgttgaaa ccccatacgc cgacggcacc gccgtagatg
     3901 ccgcgtttgc tcggttcgac ttcttcgatg atttccatgg cgcggacttt gggtgcgccg
     3961 gagagtgtgc cggcagggaa ggtagcggcg aggatgtcca tgttggtcat gccgtctttc
     4021 agacggcctt cgacgttgga aacgatgtgc attacatggg agtatttttc aatcaccatt
     4081 ttgtcggtaa ctttgacttc gccggtttta ctgatgcggc cgacgtcgtt gcgtcctaag
     4141 tcaatcaaca tgacgtgttc ggcgatttct ttggcatcgc ttaacaaatc ttgttcgttg
     4201 gcaaggtctt cggcgggggt tttgccgcgc aggcgcgtgc cggcgatggg gcggacgatg
     4261 acatcgtcgc gttcgcggcg gacgaggatt tcgggcgagg agccgacgat gtggaaatcg
     4321 ccgaaatcgt agtaaaagag ataaggcgaa gggttgagcg tacgcagggc gcggtagagg
     4381 gcgagcgggc tgtcggtgaa ttccatgctc atgcgctggc tggggacaac ctgcatgcag
     4441 tcgcctgcga agatgtagtc tttgattttg ttaacgcagg ctttgaacgg ctcttcgccg
     4501 aattcgctga cggcttcggt gtgtttgctg ccgagcgaga gcgggatggc gcagctttgg
     4561 cgcaactggg tgcggatgtc ttcgaggcgt tcgcgggcgc gttcgtagcc gtcgggctgc
     4621 gacggatcgg cgtaaacgac gagatggatt ttgccgctca aattgtcgat caccgccaat
     4681 tcttgcgaca gcatcagcaa gatgtcgggc gtgccgagcg ggtcggcttt ggtggtgttt
     4741 ttcaggcggt gggcgaagtg ttcaaaattg tagatggttt cgtaaccgaa gtagccgacc
     4801 agtccgccgg taaagcgcgg caggcttggg atttcgggtg ttttgaagcg gttgtggaag
     4861 gcttcgataa agggcagggg gttgccgtcg tgttgctcga caatttcgcc gttttgataa
     4921 acatcgacgt gtttgccgct ggctttgaga tagtggctgc aaggcaggcc gataaaagaa
     4981 tagcggccga aacgttcgcc accgacaacg gattcgagca ggtaggtata ggggcggttg
     5041 gcgagtttga gatagaggga aagcggcgta tccaaatcgg caaggagttc ttgcacgagc
     5101 gggatgcggt tgtagccttg ggcggcttgg gcttggtatt cttgtttgct gatcatttct
     5161 gctttcccaa agggcggttt cggacggcgc ggcaacgggc gcgagtatag cattttatcg
     5221 gaattgttga cagtctgacc ggagatgccg tctgaaaagg acgggcttac ggctttgttt
     5281 tgcgctgccg gtattgtttc catttaaaca gataacctgc ggttacggga atcagggcta
     5341 agatggtttt gaacgtccat gatgcatacc aaggtaaaga accgggtgcg accgattgat
     5401 tcagatacgg ccaccaacag gcagtcaggc agccgagcag cagcggccag atatgccggc
     5461 tgaggttttt ttgccaaagg aggaatgatg cggcagacca aagcagggta agggcagtga
     5521 tttttaacac ggtataagca tccgctccaa acagggcggc gagaaaatac gtaccggcaa
     5581 tccagaggac ggcaagtatc aggacaatga tttcttcagg tcgtttgaac atttttttct
     5641 tcctgtttga tttcagacgg cattgccgtt ctgtttggtt tccagcagct cccagcgttc
     5701 cagcttttcc aaaagcagca tttcgatttc ttcggcgcgg ctttgcagtg cgccggcttt
     5761 ttcgtaatct ttgaagattt cgggatcgga aagctgcgcg ttgatttctg cctgttcggc
     5821 ttccaaggcg gcgatttcgt cgggcagggc gtcgagttcg cgctgttctt tgtaggacag
     5881 tttgaccgtg cggttggctt tgggtttttc tttcaccggt tcggcatcgg ctgttttggg
     5941 tgcggattcc gtctgaatct tcgcttcgcg tgattttgcg tcgatgtagt cctgatagcc
     6001 gccgatatat tcttttaacc ttccatttcc ttcaaaaaca atgctttggg taatcacgtt
     6061 atcgaggaac atacggtcgt gcgagacaag gaataccgtg ccttgataat cgcgcaacag
     6121 gtcttcgagc agctcttggg tgtcgatgtc caagtcattg gtcggttcgt ccaagaccaa
     6181 aatattggcg ggacgggtaa agagttttgc cagcaaaagg cggttgcgtt cgccgccgga
     6241 gagtgaagaa acaggacttt gcgcgcgggc aggggggaac aggaaatctt ccaaatagct
     6301 catcacatgc tttttcttac cgccgacttc aacgtaatca ttgccctgtc cgagcgtgta
     6361 aaacacggtg tcgttttcat tcaacgcgct gcggaactgg tcgaaatagg cgacttcctg
     6421 cttgctgccg atacggattc tgccgtaggt cggctgcaat tcgcccaaaa tcagttttaa
     6481 aaatgtggtt ttgccgatgc cgtttggacc gattaagccg attttgtcgc cgcgctgcaa
     6541 gatggcggag aatttgtcca taatgacttt gtcgccataa gcaaacgagg cgtgttccag
     6601 ctcggcgatg attttgccgc ttttctcgcc gctgtcgagc ttgaaattga cctgcccttg
     6661 tacgttgagg cgttcggcac gctggcggcg cagttcttcc aaacggcgca cgcggccttc
     6721 gttgcgggta cggcgcgctt cgatgccttt gcgtatccac gcttcttcct gcgcgtggaa
     6781 tttgtcgaag aggcggttgt gttccgcttc gactgccaac tcttgcgctt ttttctcgct
     6841 atatttggag aacgagccgg gataggaacg caaaataccg cgatcgagtt cgacaatccg
     6901 cgtggcgata ttgtccaaaa aacggcggtc gtgggtaatc acaaccaagc tgccttcaaa
     6961 cgctttgagc agattttcca gccaaataat cgcgtcgata tccaaatggt tggtcggctc
     7021 gtccagcagc aatacgtcgg gcttttgcac ccaagcctgc gccaaggcga cgcgcttttt
     7081 ctgaccgccg gaaaggttgc cgattttttc attttccggc aaaccgagtt cccccaaagt
     7141 ctgcttgact gccgcatcca gtttccagcc gtccttcgct tcgattgcaa gttgcaattc
     7201 gttgagttct ttcaacaaag cctcactcga accattttcc aactcatggc tgacatgatg
     7261 ataacggcgc aataaatcgc gaatttcgcc caaaccttcg gcaacagtat caaatacggt
     7321 tgcttcctta tcaaaaaagg attcctgcgg tacataaacg attttgaggt tgttttgaac
     7381 aataatctgc ccgtcgtcga gcttttgcac accggcgagg atttttaaaa acgaagactt
     7441 gcctgcgccg ttgcgtccga tcaagccgac tttttcgccg ctgtcgagtt gaaaagaagt
     7501 tttgtcgagc aaggcaacgt ggccgatggc aaaagaagcg ttttctacag ataatatatt
     7561 catgatacaa attctcaaca gttaccgttt ggattttacc gcaagtttgg cgcgggcaat
     7621 ttcaaccgca cccggcagga cggaaacaat aatgatgccg cccatcacca agcccagatt
     7681 gttttttacg acggggaagt tggcaaagaa atagcccgcg taagaaaaca ggataaccca
     7741 caacaagcca ccgatgatgt tgtagcggat aaatttggca tagtgcattt tccccatacc
     7801 agcgacaaag ggggcgaagg tgcggacgat gggtacaaaa cgggcaatga tgatggtttt
     7861 gccgccgtgt ttttcgtaaa aacggtgggt tttatcgaga tattcgcgtc ggaagatttt
     7921 agaatcgggg ttggcgaaca gcctgccgcc gaaatatttg ccgacggtaa aattgagcgc
     7981 gtcgccgagt atggcggcaa ggcttaataa tgcaaccatc aaatgaatat ccataccgcc
     8041 cagcgcggca atcccgccgg cggcaaacag cagcgaatcg ccgggcagta agggcgtaac
     8101 aatcaggccg gtttcgcaaa aaacaatcaa aaacagaatc gcataaatcc acacaccgta
     8161 ttgcgccgac agcgcaagca ggtgttggtc gatatggagg atgaagtcga tggcggaggc
     8221 aagcacggcg cgttcctaaa aaaaacaaac cgcgtatttt aaccgattgg aaaaatgccg
     8281 tctgaaattt tcagacggca tttgttttta aaaaagttta cgcgccgtaa acggggtatt
     8341 tgtcgcagag ggcggtaatc tgtttgcgga cgttttccag attcgcttcg tcttcggggt
     8401 tagacaatac gtcggcaacc aagttcgcca atacgcgtgc gtcggcttcg ttaaaaccgc
     8461 gtgtggtcat ggcagcggag ccgatgcgga tgccggaggt aacgaagggt ttttccggat
     8521 cgttcggaat ggcgtttttg ttgacggtga tgtgcgcttt gcccaaagcg gcttcggcgg
     8581 ctttgccggt aattttcatc ggttgcaggt cgacgaggaa aacgtggctt tcggtgcggc
     8641 cggaaacgat gcgcaaaccg cgtttgacca gttcttccgc catggcggca gcattgattt
     8701 tcacttgttt tgcgtattgt ttgaactcgg gttgcaacgc ttctttaaac gctacggctt
     8761 tggcggcgat gacgtgcatc agcggaccgc cttgcaggct cgggaagatg gaagagttca
     8821 gtgctttttc gtgtgtgttg tcgcggcaca gaatcacgcc gccgcgaggg ccgcgtaggg
     8881 ttttgtgggt ggtggtggtc acgaagtcgc agaatggcac ggggttggga tattcgccac
     8941 cggcaatcag tccggcgtag tgcgccatgt cgacaaagag gtatgcgccg actttatcgg
     9001 cgatttcgcg gaattttgcc cagtcgattt gcaacgcgta ggcagacgca ccggccacaa
     9061 tcattttagg tttgtgttcg agagccaagc gttccacttc ggcataatcg agcacttcgt
     9121 tttcatccaa accataagta acggcattgt agagtttgcc ggaaatatta acgctcgcgc
     9181 cgtgggtcag gtggccgccg tgcgccaaag acatacccaa aatggtgtcg cccggtttta
     9241 aaacggaagc gtacacggct tggttggctt gcgagccgga gtgcggttga acgttggcgt
     9301 attgcgcgcc gaacagtttt ttgacgcggt cgattgccaa ttgttcgaca atatcgacgt
     9361 attcgcagcc gccgtagtag cgtttgccgg ggtagccttc ggcgtatttg ttggtcagct
     9421 gggaaccttg cgcttccatt acggcgcagc tgacgtagtt ttcagaggca atcagctcga
     9481 cgtggtcttg ctggcgttgg tcttcttggg caatggctgc tgccaaatcg gggtcatatt
     9541 gtgcgagggt aacgcttttt gaaaacatgt tctcggctcc tttgtgtaat cagggtatca
     9601 tgagtgtttt ttgtataaaa aaatacttca aaacctaagg cagatagtcc ataatgcgta
     9661 aattttcttt ggcattatca ggtaatttat ttaacatgct ggtttttagc gtctcattac
     9721 ctttatgtta gtacaaggct acccaaaggc aatgcatcaa atggtaaatt ttcggcagtt
     9781 tgtgcaaaat caatcaaata accagcacca accacatctt tatcagccat aacccgctga
     9841 tacaccactt gccaagacaa ctctcctgcc ccaccatgtc ttaaaatcag atgaatggct
     9901 tgtaatgctg tgtttggcgt ctcacaaatt gcgaagatct gctcagaagc ttgcttgatg
     9961 gcttgtattt ttgtttgatt actcacaatt tccccctatt ttaatattaa cttaatgcgg
    10021 tcaaattcac aaaatacaag cttcacctct aatcacccat cactcgacct tctcggcgtg
    10081 gatcggcacc accaaccagc ctgcttggct cgataataat ggcttgaaca cctgaattta
    10141 gctcacgcac gtcagtctta tagcccaaat catttaatgc ttgttgccac tggagggcgg
    10201 ttgtacccgt ttctagttca tagctaccaa agcgatttaa taaattgggt gcactgatgg
    10261 catcttggat atccatattc cagtcactat gtgccacaat cgtcttagcg acatagccaa
    10321 tgatacggct accacctggg gagccgattg ccatataagg cttgcctgct ttaaatacga
    10381 tggttggtgc cattgaggag cgtggtctct tgccgggctc gacacgattg gcgacctgtt
    10441 tgccctgctt tattggctca aaactaaagt ctgtcagctc attattcagc aggtagccat
    10501 ttgccatcaa agttgagcca aacgcatttt caatggaagt cgtcattgat agcacattgc
    10561 ccgccttatc cacaattgat atatgactgg tagaaggtaa ctcaatcgct tgtgaggaca
    10621 cccactcatg aataaaatcg cctgcagata cgctaggcaa tgccttatcc gactgctcaa
    10681 gcagctggct gcgatgtttt aggtagtctt tagaaatcaa ctggcgaatg ggtactggta
    10741 caaaatcagg gtcgcccaaa tatacatcac gatccgcaaa cgcaagccta gaagcgtcgc
    10801 ccaagagacg taaaccttca gcatcatacc ccacctgatt gggtgaaaat tcatttaaaa
    10861 tccccaaaat ctgacccaca gcaatcccac ctgagcttgg tgcacccata ccgcatactt
    10921 cataaatacg ataagtcaca caaacaggcg ggcgttccac cacttgataa tcagataaat
    10981 cttgtaagga taattgaccg gggttatcct tagcattttg gacaactgaa acgatatttt
    11041 gggcatattt accagtatgc agagcttttg caccttgagc tgctaacgcc tgaacactgt
    11101 cagcaaattc taaatttttc agcaagctgc ctgcttgtag cggcacacca ttcggcaaaa
    11161 aataagcggc tgtttttgga tagcgtgcca aatgctgctg attttgctca accgagatgg
    11221 caagccttgg cgacacctca aagccttgtt ttgccaagcg gatcggtgta tcaaataatt
    11281 ttccccaagg caatacaccg tatcgctgat gtattgtctc catcagttta gggatagcag
    11341 gcgtacccac cgagcgacca ccgaccaccg cttccataaa tttcaatggt tgaccatctt
    11401 tatccaaaaa taattccggc gtcgcacgca tcggtgccgt ctcacgccca tcaaatgtgg
    11461 tcaatgtttt ggcggtatta tcccaataca acacaaatgc accaccgccc aagcctgacg
    11521 actgtggctc taccaagctt agtgtcgtct gcaccgccac catcgcatct gcagcgctac
    11581 cgccttgctt taagatatca tagccagctt gtgttgctaa tggattggct gacgctacca
    11641 taaaatcact tgcaatcacc tgcttttgtt cggtcagtcc cgttgcatgt tcaggcgtgt
    11701 gagcgtctgc acctgtgatg acagcagaat gagtattaac cttaccttga ttggcatgga
    11761 tgacttgaca tccagagatt gtcatagaca ttatcaatgc agtcaataaa tatgttttag
    11821 ccacaagcac tccttcgcct gagtttgatt gatgattcat acaaggcatg ctgattattg
    11881 tatttaatat ggctaaataa ttcaatccaa actatcaatc ttgaccatca aaaaaagacc
    11941 gctaatgtca tcagcagtct tttttgatat ttattttaag atattaagta atcagacctt
    12001 tgggctatgc tcttcaatga gtggttttag ctcacctgat tggtacattt gtaggataat
    12061 atcactacca ccgattaact caccattaac ccaaagctgt ggaaaggttg gccgattggc
    12121 gatgagtggt agagtactgc gaatttctgg gttttctagg atattaacaa aagcaaaggg
    12181 tctgccaatt gggtcagcac ctctactgca cgcgctgaaa atccacattg gggaaactgg
    12241 ggcgtgcctt tcat
//
LOCUS       DAXHRI010000044        12202 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXHRI010000044 DAXHRI010000000
VERSION     DAXHRI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.044"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            207..944
                     /locus_tag="ACFM75_000920"
     CDS             207..944
                     /locus_tag="ACFM75_000920"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC6255496.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            947..1633
                     /locus_tag="ACFM75_000921"
     CDS             947..1633
                     /locus_tag="ACFM75_000921"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC6255497.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSIG
                     NALARKLDKR"
     gene            1792..2655
                     /locus_tag="ACFM75_000922"
     CDS             1792..2655
                     /locus_tag="ACFM75_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6255498.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2717..3286)
                     /locus_tag="ACFM75_000923"
     CDS             complement(2717..3286)
                     /locus_tag="ACFM75_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC6255499.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3444..4304
                     /locus_tag="ACFM75_000924"
     CDS             3444..4304
                     /locus_tag="ACFM75_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC6255500.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4439..4777
                     /locus_tag="ACFM75_000925"
     CDS             4439..4777
                     /locus_tag="ACFM75_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255501.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNESIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5145..5498
                     /locus_tag="ACFM75_000926"
     CDS             5145..5498
                     /locus_tag="ACFM75_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC6255502.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5488..6354
                     /gene="atpB"
                     /locus_tag="ACFM75_000927"
     CDS             5488..6354
                     /gene="atpB"
                     /locus_tag="ACFM75_000927"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC6255503.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6411..6647
                     /gene="atpE"
                     /locus_tag="ACFM75_000928"
     CDS             6411..6647
                     /gene="atpE"
                     /locus_tag="ACFM75_000928"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC6255504.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6718..7188
                     /locus_tag="ACFM75_000929"
     CDS             6718..7188
                     /locus_tag="ACFM75_000929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC6255505.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7193..7726
                     /locus_tag="ACFM75_000930"
     CDS             7193..7726
                     /locus_tag="ACFM75_000930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC6255506.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7737..9284
                     /gene="atpA"
                     /locus_tag="ACFM75_000931"
     CDS             7737..9284
                     /gene="atpA"
                     /locus_tag="ACFM75_000931"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC6255507.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9309..10184
                     /gene="atpG"
                     /locus_tag="ACFM75_000932"
     CDS             9309..10184
                     /gene="atpG"
                     /locus_tag="ACFM75_000932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC6255508.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYQNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10222..11619
                     /gene="atpD"
                     /locus_tag="ACFM75_000933"
     CDS             10222..11619
                     /gene="atpD"
                     /locus_tag="ACFM75_000933"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC6255509.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11630..12052
                     /locus_tag="ACFM75_000934"
     CDS             11630..12052
                     /locus_tag="ACFM75_000934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC6255510.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         2999 a         3122 c         3151 g         2930 t
ORIGIN      
        1 ttttgttaat ccactatatt gtccgaaatg gttgcccgct tcctgcctta ttgacaaaaa
       61 aacgctttcc cgataatatc ctacgaaaat taacctgccg atttgacaca gcttgcgggc
      121 ataacagcta aagcgttccg acaatttcag ctttatcttc cgcgcccgtt gtgtccgaca
      181 tcgggctttg ttgtatggga aagacaatga ttattttgga caaggtttcc aagcattacc
      241 aaacgcgcga caagacccgt tttgccgccg tcgagccgac cagcctcgaa atccgcgacg
      301 gcgaaatctt cgggctgatg ggttattcgg gtgcaggcaa atccaccctg ttgcgcctga
      361 ttaacctgtt ggaacgcccc gacagcggca aggtcaacgt ctgcggacaa gagctgaccg
      421 cgctcgatgc cgccgcattg cgtcaggctc ggcagaatat cggcatggtg tttcagcagt
      481 tcaacctgtt gaacaaccgc accgtcgccg gcaacgtcgc cttcccattg gaaatcgccg
      541 gatggccgtc tgaaaaaatc aaagcgcgcg ttgccgaatg tctcgaaatc gtcggactga
      601 ccgaacgcgc cggccactat cccgcccagc tttccggcgg acaaaaacag cgcgtcggca
      661 tcgcccgcgc gctcgcatcc aagccgcaag tcatcctcgc cgacgaaccc acttccgccc
      721 tcgaccccgc caccacgcgc agcgtcttgg aatgtttgga agacatcaac aaacgattca
      781 acgtaaccat cgtcatcgta acccacgaaa tgagcgttat cagacgactg tgcgaccgcg
      841 ccgccctgtt ggataaaggc aaagtcgtcg aaatcgtcga agtacgcggc aaccaaatcc
      901 acgcccgatc cgacatcggg cgcgaactga ttcgggagga ctgaatatgg caggcttaac
      961 atttgaacaa accgtttcca acatcatcgg tatgaaaggc gaaatcgtcc gcgcattggg
     1021 cgaaaccttc gtaatggtcg gtttgtccac cacattcgcc gtcgttttcg gcacgttgct
     1081 gggcgtgttg ctcttcgtaa cctccagccg ccaactgcat tacaacaagc cggtaaattt
     1141 cctgctcgac aacctcgtca acctcatgcg cgccttcccc ttcgtcattt tgatgattgc
     1201 gatgataccc gccacacgcg ccattgtcgg cagcaccatc ggtccggttg ccgcctcgct
     1261 ggtattgagc gtgtcgggat tgttttattt tgcccgactg gtggaacaaa acctgcgcga
     1321 agtccccaaa ggcgtgattg aagccgccgc cgcgatgggt gcgcctccga ttgccatcgt
     1381 ctgcaaagtc ctcttgaacg aagcgcgcgc gggcatggtt tccagcatta ccgtgcttgc
     1441 catcgggctt ttgtcataca gcgcggcggc agggatgata ggcggcggcg gcttgggcga
     1501 cctcgccatc cgctacggct actaccgcta ccaaaccgaa gtcatcgtct tcatcgtcgc
     1561 cctcctcgtg ctgctcgtta tcctgattca aagcatcggc aacgcgttgg cgcggaaact
     1621 cgacaaacgt tgaacccgaa tgccgtctga acgccaaaac ccccaccgct atccgaaaaa
     1681 tgctataaaa tccccctgtt cgcggcaaat gccgtctgaa cgccgaatct ggacggcagg
     1741 actccctgcc cgtcattttt gtttgaaacc gccacaacat caggagaaaa tatgaaaacc
     1801 ttcttcaaaa ccctttccgc cgccgcactc gcgctcatcc tcgccgcctg cggcggtcaa
     1861 aaagacagcg cgcccgccgc atccgcttct gccgccgccg acaacggcgc ggagaaaaaa
     1921 gaaatcgtct tcggcacgac cgtcggcgac ttcggcgata tggtcaaaga acaaatccaa
     1981 gccgagctgg agaaaaaagg ctacaccgtc aaactggtcg agtttaccga ctatgtacgc
     2041 ccgaatctgg cattggctga gggcgagttg gacatcaacg tcttccaaca caaaccctat
     2101 cttgacgact tcaaaaaaga acacaatctg gacatcaccg aagtcttcca agtgccgacc
     2161 gcgcctttgg gactgtaccc gggcaagctg aaatcgctgg aagaagtcaa agacggcagc
     2221 accgtatccg cgcccaacga cccgtccaac ttcgcccgcg tcttggtgat gctcgatgaa
     2281 ctgggttgga tcaaactcaa agacggcatc aatccgctga ccgcatccaa agcggacatt
     2341 gccgaaaacc tgaaaaacat caaaatcgtc gagcttgaag ccgcgcaact gccgcgcagc
     2401 cgcgccgacg tggattttgc cgtcgtcaac ggcaactacg ccataagcag cggcatgaag
     2461 ctgaccgaag ccctgttcca agaaccgagc tttgcctatg tcaactggtc tgccgtcaaa
     2521 accgccgaca aagacagcca atggcttaaa gacgtaaccg aggcctataa ctccgacgcg
     2581 ttcaaagcct acgcgcacaa acgcttcgag ggctacaaat cccctgccgc atggaatgaa
     2641 ggcgcagcca aataaggcag tcgtataaaa tgatgccgtc tgaaccgtat ccgtgttcag
     2701 acggcatttt tgtcctttaa tccgccattc cctgccattc cgccgaatcc ggcgtatcga
     2761 ttccgaacag cgacaaagcg tgtgcgacac tgtgcgccac tatgtcgtcc gccgtctgcg
     2821 gtttgcggta catcgcagga acagggggaa acaccacgcc gcccatttcc gttacccgct
     2881 tcatattgtc caaatgggca aggttcagcg gcgtttcgcg caccatcagc accagccgcc
     2941 gcctttcctt caaaaccaca tccgccgcac gcgtcaagag gttgtcgccg aagccgtgcg
     3001 cgaccgaggc aagcgtccgc atcgaacagg gggcgaccag catcccgtcc gttttaaacg
     3061 taccgctggc aatgcacgcc ccgatattgc cgatcggatg cacgaagtcc gcgagggcat
     3121 atacctcgtc tctcgcataa gccgtttccg aagcgcgcgc catctccgca cctttcgata
     3181 ccacaaggtg cgtttcgata tcttgcgcgc gtaaaagttc caaagccttc acgccgtatt
     3241 ggaaaccgct cgccccactg atgccgatta tcaaacgccg taccatcatc cgcctttccc
     3301 ataaaaccgc ctgcaacggc aaaccggcta ttatagtgaa aaaacagaaa tccgataaat
     3361 acggatacaa attgtcggca acacccaata tccgataaaa tatccgattt aacatcctat
     3421 ctgaataggc acgggagggc ggtatggcaa aagtaaaagg cggattgggg cgcggtttgg
     3481 attcgctgct cgccaacggc gcagacaaca gcagcggcga ccgattgacc acggttgcgg
     3541 ttaaagatat ccggcccggc cgctatcagg cgcgtgttca aatcgatgac gaagccttgc
     3601 aggagctggc agattcgatt aaggcgcaag gcgtgataca gcccgtcatc gtgcgcgaac
     3661 acggactgtc ccgatacgaa ctgattgcgg gggagcggcg ttggcgtgcc gcacagattg
     3721 ccggcctgac cgaaatcccc gccgttatca aaaccatcag cgacgaaacc gcgttggcaa
     3781 tgggtttgat cgaaaacctc cagcgcgaaa acctcaaccc catcgaagaa gcacaaggct
     3841 tgaaacgcct tgccgacgag ttcgggctga cccacgaaac catcgcccaa gccgtcggta
     3901 aaagccgaag cgcgatttcc aacagcctgc gccttttaag cctgcccgaa cccgtgcagg
     3961 aaatgcttta ccaacgccgc ctcgaaatgg ggcacgcccg cgcactgctg accctgcccg
     4021 tcgtcgaaca gctcgagttg gcgcaaaagg ccgtcaaaaa cggctggtcg gttcgcgaag
     4081 tcgagcgccg cagccaagcc gcccttcaaa acaaacgtcc cgagcccaaa aaaactgccg
     4141 ccgccgacat cggccgcctg aatgatttgc tgactgaaaa actgggtgtc aacgccgaaa
     4201 tcaaaaccgc caaccataaa aaaggcagaa tcatcctgca tttcgacacg cccgaaacct
     4261 tcgaccatat cttgaagcag ttgggcatag attaccgacc ttaattttgc gggatatacc
     4321 gtctgaaata tagagaatag ctttccagat tgtaagtggg aaatataatt ctattgacat
     4381 ttttctgctt cacgtaagaa tcgttttcct gttttcattt ttaattttcg aggaaattat
     4441 gaacacacgc atcatcgttt cggctgcgtt cgttgcgttg gcattagcag gttgcggctc
     4501 aatcaataat gtaaccgttt ccgaccagaa acttcaggaa cgtgccgcgt ttgccttggg
     4561 cgtcagccca aatgccgtaa aaatcagcaa ccgcagcaat gaaagcatac gcatcaactt
     4621 taccgcaact gtgggtaagc gcgtgagcca atgctatgtt accagtgtaa tcagcacaat
     4681 cggcgttacc acttccgatg caatttgttt gggaggcgga acgcacaaag gcaaaagtca
     4741 atgcaatgct ttgcttaaag cggcaggccg ttgctaatcc tttattcgga aaaggtcgtc
     4801 tgaaaatatt ttcagacgac cttttattta ttgagcaaat tccccaaact gccgacaatc
     4861 cgaccgataa actgctacaa tttcgcgttg attttttatt atttccacgt aaagcaaaca
     4921 aatcacgtaa gaacattaaa aatgtaaaga aattgcggac aaactcctta atatcctgta
     4981 attatatgta atgtgtgtta gaatgacacc tgtcgccgtt attcaggcgg cgagcgcggc
     5041 ggattgggat acgttgtttc acaaagatta actgtcgctt gacgtaataa ggcttctaaa
     5101 ttatattagc cgcgccccaa atccgaggcg gaaacattcc ctttatgaag cagattatca
     5161 tcctacaatc cgccgtattg tccatatgcg ccgcagttgc ctttgctgta tggggttttg
     5221 ccggattcct ttcggcggtc ggtggcggtt tgtcctacct gcttcccacg tttgttgcag
     5281 ttttactttt aaaacttttc aggggaaacc ccttcctgca aagcaggatg ttcgtcttcg
     5341 gagagatttt aaaagtagtg ctgtcgctgt tgtccatgct tgccgtattt gcaatatggc
     5401 atcaatcgct ggtgttcgcc ccgtttctga tggggctgct cggtgtcagc catttggttt
     5461 ttttagtatt gttgagagtg aaagattatg gcaggtgaaa ccattaccgc tgccgactac
     5521 atcaagcacc acttgcagag cttgaccagt ttgtcggatg ttactcaggg tcagggactg
     5581 aaaaacattg ctgatttttc gtttattaac cttgatgccg tcttttttgc cgtcctgttg
     5641 ggcgtaatcg gcagctttct gttgtggcgc ggtgcaaaaa aagcgacggc aggcgttccc
     5701 ggacgtttcc aggccgcagt ggaaatcttg tttgagtttg tggacgatat gtgtaagagc
     5761 atcattcaca acgaaaagtc tagaaaagcc gtcgcgccgt tgggtctgac gctgtttgtc
     5821 tggatttttc tgatgaacgc gatggatatg ctgccggtcg atttgctgcc gatggtatgg
     5881 cagggcatta ccggcaacca tcacgccctg ctgcgcgtcg taccgactgc cgatttgaac
     5941 accactttgg cactcgctgt cggcgtgttg ctgatttgta tctattacaa catcaaaatc
     6001 aaaggattgg gcggctggtt tcacgagttg ttcagcgcgc cgttcggtgc aaaactcgca
     6061 cctgcgaact ttctgttgaa tttggtcgag tttctttcta aaaccgtatc ccacggtatg
     6121 cggttgttcg gcaatatgta tgcgggcgaa ttggtattct tgctgatagc cttgcttggc
     6181 ggtgcttggg cggcttccgg cagcgtcgaa gtcatggatc cgattctgtt tgtattccac
     6241 attattgccg gtttggcatg ggcgattttc catattttgg tgattaccct gcaggcgttt
     6301 attttcatgg cgttggcgtt cgtctatatc ggacaggcac atgatgcgca ctgattttcc
     6361 taagtagttt gtttttgtag tttaaccttt gtttctttaa ggagtttaaa atgggtttga
     6421 ttgctatcgc atgtggtttg atcgttgcat tgggtgcatt gggtgcctct atcggtatcg
     6481 caatggtcgg ttctaaatat ttggagtctt ctgcccgtca gcccgaactg atcggtccgc
     6541 tgcaaaccaa actgttcctg attgccggtt tgattgatgc cgcattcttg attggtgtcg
     6601 ccattgcact gctgttcgcc ttcgttaacc cgtttgcagg tgcataatca ggacggattc
     6661 gtccgttccg tacaggcgca gcctgtttga ttaaccccag tacgaaggtt aagtaacgtg
     6721 aatatcaatg caacattatt cgctcaaatc atcgtctttt tcggtttggt atggtttacc
     6781 atgaaattcg tgtggccgcc gattgcaaaa gctttggatg agcgtgccgc aaaagtcgcc
     6841 gaaggcttgg ctgccgccga gcgtggtaaa agcgatttcg agcaggctga aaaaaaggtt
     6901 gcagaactct tggcagaagg gcgtaatcag gtttccgaaa tggttgccaa cgccgaaaaa
     6961 cgtgccgcca aaattgtcga agaagccaaa gaacaggctt cttccgaggc ggcgcgcatt
     7021 gcagctcagg caaaggccga tgtggagcag gaattgttcc gcgcacgcga atccctgcgc
     7081 gagcaggttg ccgtgttggc tgtcaaaggt gccgaatcta ttttgcgcag cgaagtcgat
     7141 gcttccaaac acgcaaaact gctcgatacc ctgaaacagg agctgtaatc ttatggcaga
     7201 gttcgcaacg attgccagac cttatgcaaa ggcattgttc ggtctggctc aggaaaaaaa
     7261 ccaaattgag tcttggttgg gcggactgga aaaacttgcg gcggttgttc aggaagggaa
     7321 ggtggcttca ttgattgacc gtcctgaaac gaatgcttca gaaaaagcag atatcctcat
     7381 cgatttggtc ggtttgaaag acaaggagtt gaaaaacttt gttatcgtct tggccgggca
     7441 gaaacgtttg tcgatattgc cggaagtata tgctcaatat caagacttga ccttatcatt
     7501 caaccatatc aaatctgccg tcatttacag tgcctatccg ttgaccgaca aacaggtcgg
     7561 cgagttggcg caaatgctga ataagcgttt cgacagcgag ctgaaaatct ctgtcgaaat
     7621 cgaaccggag ctgattggcg gcataaaagt tgaagtgggt gatcaggttt tggatttgtc
     7681 tgtacaaggc aaattgagtg ccttgtacac gactatgacg aattaggaga gttttcatgc
     7741 agcttaatcc tgctgaaatt agcgatttga ttaaagccaa gatcgaaaat ctgtctgtaa
     7801 atgccgaagt gcgtacccgt ggtacggtga tttcggtaac tgacggtatc gttcgcatcc
     7861 atggtttgtc agatgcaatg caaggtgaga tgctcgaatt cccgggtaac actttcggct
     7921 tggcgatgaa cctggagcgc gactccgtcg gcgccgtagt gttgggcgag tacgaacata
     7981 ttaaagaagg cgacacggtt acctgtaccg gtcgtatctt ggaagtgccg gtcggacgcg
     8041 aactggtcgg acgcgtcgtt gatgcattgg gtcggcctat cgacggcaaa ggcccgatta
     8101 atacaacttt gaccgcccct atcgaaaaaa tcgcaccggg cgtgattgcg cgcaaatcgg
     8161 ttgaccagcc gatgcaaacc ggtctgaagg cgattgactc tatggttcct gtcggtcgcg
     8221 gtcagcgtga gttgattatt ggcgaccgtc aaaccggtaa aacggccgta gcgctggatg
     8281 ccatcgtcaa ccaaaaaggt acgggtgtta tctgtatcta tgtcgctatc ggtcaaaaag
     8341 catcttctat tgccaacgtg gtccgcaaat tggaagagca tggcgcgatg gaacacacga
     8401 ttgtggttgc tgcaactgca tctgaagcgg cggcattgca atatatcgca ccttactccg
     8461 gttgtacgat gggtgaattc ttccgcgacc gcggcgaaga tgccttgatt gtttatgacg
     8521 atttgtccaa acaggctgtg gcttaccgtc aaatttccct gcttttgcgc cgtccgcccg
     8581 gccgcgaagc ctatcccggc gatgtgttct acctgcactc ccgtctgttg gaacgtgcag
     8641 cacgtgtcaa tgaacacgaa gtggagaaat tgaccaacgg cgaagtaaaa ggcaaaaccg
     8701 gttctctgac tgctttgccg attatcgaaa cccaagcggg cgacgtatct gctttcgttc
     8761 cgactaacgt catttcgatt accgacggtc agattttctt ggaaaccgac ctcttcaacg
     8821 ccggtatccg tcctgcaatc aatgccggta tttccgtatc ccgcgtaggc ggtgctgcac
     8881 aaaccaaagt gattaaaaag ctgggtggcg gtatccgttt ggcgttggca caatatcgtg
     8941 aattggcggc gttctcgcaa tttgcatccg atttggatga agctacgcgc aaacaattgg
     9001 agcatggcga agtcgtaacc gaactgatga aacagaaaca gttcagtacg ttgaatacgg
     9061 ctgaaatggc tttgaccctt tgggcaatca acaacggttc gtattctgat gttccggttg
     9121 ccaaagctct ggcttttgaa tctgaatttt tgagctttgt ccgtacccaa catccggaag
     9181 ttttggaagc cgtcaatgct tcaggtgcaa tgtccgacga gagcgagaaa acgcttgaag
     9241 cagccatgaa atccttcaaa tcttcttacg cctatcaggc ataaggcttg aagtgaaagg
     9301 agtctgaaat ggcagtagga aaagagattc tcaccaaaat ccgcagtgtt cagaataccc
     9361 aaaagatcac taaagcgatg cagatggtgt caacctctaa aatgcggaag actcaggaac
     9421 ggatgcgttt ggcgcgtccg tatgccgaaa aagtgcgtat ggtgatgagc catcttgcgc
     9481 aaaccaatac cgatcatggt attccgttac tggaatctca tcgggaaatc agacgtgtcg
     9541 gttttatttt gattacgtct gataagggtt tgtgtggcgg tttgaacgcc aacgtgttga
     9601 aaaagttttt ggcacaagtt caagagtatc agaatcaagg tattgaagag gaaatcgtat
     9661 gccttggcag taaaggtctg atggcgtgtc agagcattgg tctgaatgtg gttgccagtg
     9721 ccgtaaattt gggcgatacc ccaaaaatgg aaatgctgct cggacctttg acagaactct
     9781 tccaacggta tgagaaacat gaaattgaca gaatccatct ggtgtattcg ggttttgtca
     9841 ataccatgcg tcaagaaccg agaatggaag tattgctgcc tatcggtgag aacgtgattg
     9901 gcgattcagc tcccaaatca ccgttcagct gggaataccg ctacgaaccg actgcacttg
     9961 cagtgttgga atatctggtt cgccgctatt tagagtctgt ggtttatcag gcgttgagcg
    10021 acaatatggc atccgaacag gcagcccgca tggttgccat gaaagctgca acagacaatg
    10081 caggcaatgc catcaaagag ttgcgtttgg tatataacaa atcgcgtcaa gctgcgatta
    10141 ccacggaatt gtcagaaatt gtagcaggtg cggcggctgt ctgatgccgt ctgaatcctg
    10201 tacagaaatt aggatacgat aatgagccaa ggcaaaatcg tacaaattat cggtgcggtt
    10261 gttgacgtgg aatttccacg cgacatgatt ccgcgcgttt acgacgcttt gaaattagac
    10321 gaaaacggtc tgactttgga agtccaacag cttttgggcg acggcgtagt ccgtaccatt
    10381 gcgatgggca gctcggacgg tttgaaacgc ggcatgactg tgagcaatac cggtgcgccc
    10441 attactgtgc cggtaggtaa aggtacattg ggtcgtattg tcgacgtatt gggtacgcct
    10501 gttgacgagg caggtccaat cgataccgac aagagccgtg ccatccacca agccgctcct
    10561 aagtttgacg aactgtcttc cacaaccgaa ttgctcgaaa cgggcattaa agtgattgac
    10621 ttgctgtgtc cgtttgccaa aggcggtaaa gtaggtctgt tcggcggtgc cggtgtgggt
    10681 aaaaccgtga acatgatgga attgatcaac aacatcgcca aagcgcacag cggcttgtcc
    10741 gtgttcgcag gcgtgggtga gcgtacccgc gaaggtaacg acttctacca cgagatgaaa
    10801 gattccaacg tattggataa agtggcaatg gtttacggtc agatgaacga acctccgggc
    10861 aaccgtttgc gcgtcgcatt gaccggtttg accatggcgg aatacttccg tgacgaaaaa
    10921 gacgaaaacg gcaaaggccg cgacgtattg ttcttcgtgg acaacatcta ccgttacact
    10981 ctggctggta ccgaagtatc cgcattgttg ggccgtatgc cgtctgcagt gggctaccaa
    11041 ccgacattgg cagaagaaat gggtcgtttg caggagcgta ttacctctac ccaaaccggt
    11101 tctattactt ctatccaagc cgtatatgta cctgcggacg acttgaccga cccgtctccg
    11161 gcaacaacgt tcgcccactt ggacgcaacc gtcgtattga gccgcgatat tgcctctttg
    11221 ggtatttacc cggcagtcga tccgcttgat tccacttcgc gccaactgga tccgatggtc
    11281 ttgggtcaag agcactacga cgtggcgcgc ggcgtacagt ccaccctgca gaaatacaaa
    11341 gaattgcgcg acatcatcgc catcttgggt atggacgaat tgtctgacga agacaaactg
    11401 accgtaatgc gtgcgcgtaa aatccaacgc ttcctgtccc aaccgttcca cgttgccgaa
    11461 gtgtttacag gttctccggg caaatatgtc gccctgcgcg ataccattgc cggcttcaaa
    11521 gccatcttga acggcgaata cgatcatctg cccgaacagg cattctacat ggtcggcagc
    11581 atcgaagaag cggttgagaa agcgaaaacc ttaaactgag gaggtcggca tgagcatcat
    11641 gcaagttgag gtggtcagta gcgagcaaaa catctattcg ggcgaagcca gctttgtagt
    11701 ggttccgacc gttcaaggtg agcttggtat ttacccgcga cacgagccga ttatgagttt
    11761 ggtgcggccg ggggctttgc gtttgaccgt tccgggcgag gataaagagg ttttggttgc
    11821 tgtttccggc ggtgttttgg aagtacagcc tgataaagta actgtcttgg cggatgttgc
    11881 cgtccgcagt gcggagatgg atcaggcacg tgcggaagag gcgaaaaaag ccgcagaagc
    11941 gggcatttcc caagctaaag acgataaggc tttggcggag gcgcataaag cattggctgc
    12001 tgcgattgca cagctcaaaa ctttggacta tatccgttcg cacaagaaat aaacttgttt
    12061 ggtttggaaa agcacgaccg tttggtcgtg ctttttattt tccggattta aattaaagac
    12121 tgtgtttgga tatggcgttg gcaggcatag cctgctttaa ggtattcaat agggcagcct
    12181 gccgcctaac cgaaacatat ag
//
LOCUS       DAXHRI010000045        12150 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXHRI010000045 DAXHRI010000000
VERSION     DAXHRI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.045"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            40..243
                     /locus_tag="ACFM75_000935"
     CDS             40..243
                     /locus_tag="ACFM75_000935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255511.1"
                     /translation="MKEMKPVRYHIGDMPETSKQTASRHDDRAVGVDDDCFHASFGQF
                     LFGCSAEATRTAPISVKRSCLFT"
     gene            351..1385
                     /gene="lpxK"
                     /locus_tag="ACFM75_000936"
     CDS             351..1385
                     /gene="lpxK"
                     /locus_tag="ACFM75_000936"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC6255512.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1591..2172
                     /locus_tag="ACFM75_000937"
     CDS             1591..2172
                     /locus_tag="ACFM75_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC6255513.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2241..2423
                     /locus_tag="ACFM75_000938"
     CDS             2241..2423
                     /locus_tag="ACFM75_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC6255514.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            2420..3181
                     /gene="kdsB"
                     /locus_tag="ACFM75_000939"
     CDS             2420..3181
                     /gene="kdsB"
                     /locus_tag="ACFM75_000939"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6255515.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            3204..3521
                     /locus_tag="ACFM75_000940"
                     /pseudo
     CDS             3204..3521
                     /locus_tag="ACFM75_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3623..3823
                     /locus_tag="ACFM75_000941"
                     /pseudo
     CDS             3623..3823
                     /locus_tag="ACFM75_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3980..4072
                     /locus_tag="ACFM75_000942"
     tRNA            3980..4072
                     /locus_tag="ACFM75_000942"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4014..4016,aa:Ser,seq:gct)
     gene            complement(4108..4278)
                     /locus_tag="ACFM75_000943"
     CDS             complement(4108..4278)
                     /locus_tag="ACFM75_000943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255516.1"
                     /translation="MKTGKDYAVQFFVYQNSPLFRNLPFRLVKSDFARAGTLQIQAAH
                     GRQPSALPQQSA"
     gene            4375..5160
                     /gene="trpA"
                     /locus_tag="ACFM75_000944"
     CDS             4375..5160
                     /gene="trpA"
                     /locus_tag="ACFM75_000944"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC6255517.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            5197..6069
                     /gene="accD"
                     /locus_tag="ACFM75_000945"
     CDS             5197..6069
                     /gene="accD"
                     /locus_tag="ACFM75_000945"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC6255518.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRREMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6129..6740)
                     /locus_tag="ACFM75_000946"
     CDS             complement(6129..6740)
                     /locus_tag="ACFM75_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC6255519.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGDVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            6849..7073
                     /locus_tag="ACFM75_000947"
     CDS             6849..7073
                     /locus_tag="ACFM75_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC6255520.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            7074..7220
                     /locus_tag="ACFM75_000948"
     CDS             7074..7220
                     /locus_tag="ACFM75_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255521.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            7436..8470
                     /gene="pyrC"
                     /locus_tag="ACFM75_000949"
     CDS             7436..8470
                     /gene="pyrC"
                     /locus_tag="ACFM75_000949"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC6255522.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(8838..9263)
                     /gene="nusB"
                     /locus_tag="ACFM75_000950"
     CDS             complement(8838..9263)
                     /gene="nusB"
                     /locus_tag="ACFM75_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC6255523.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(9341..9817)
                     /gene="ribH"
                     /locus_tag="ACFM75_000951"
     CDS             complement(9341..9817)
                     /gene="ribH"
                     /locus_tag="ACFM75_000951"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC6255524.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(9864..10187)
                     /locus_tag="ACFM75_000952"
     CDS             complement(9864..10187)
                     /locus_tag="ACFM75_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC6255525.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLANIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPKD"
     gene            10354..11073
                     /gene="rnc"
                     /locus_tag="ACFM75_000953"
     CDS             10354..11073
                     /gene="rnc"
                     /locus_tag="ACFM75_000953"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC6255526.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSCHTKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            11121..12044
                     /gene="era"
                     /locus_tag="ACFM75_000954"
     CDS             11121..12044
                     /gene="era"
                     /locus_tag="ACFM75_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC6255527.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVVLVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         2804 a         2895 c         3537 g         2914 t
ORIGIN      
        1 ggtttttgtt aatccactat aaatgaaaga tactggaaaa tgaaagagat gaaacctgtc
       61 cgttatcata ttggcgatat gcccgagact tcaaaacaaa ccgcctcccg tcatgacgac
      121 agggcagtgg gtgttgacga tgattgtttt catgcttcct ttggtcaatt tttgtttggg
      181 tgttcggcag aggcaacccg aaccgcgcca atttctgtaa agcgcagttg cttatttacc
      241 tgattggttc gcttatcggt ttggtcttcg cgttgtttat aggtgggtct gtatcgggta
      301 cgcatgatta atgccccggg ctgattttgc ttcgaggatt tgtatcgaat atgccgaatt
      361 gtttcaaatt tcataccgtt atcgaacggc attggcaaaa accttatccg gttttgtctt
      421 ttctgcttaa gccgctctcc gggctgtttg ccaaaattgc ggcaaaacgg cgggcggatt
      481 ttttatcggg aaaacggcaa agcgaaaagc tgtccgtgcc tgtggtcgtg gtcggcaata
      541 ttcacgcggg tgggacgggg aaaacgccga ttgttgccgc gctggtgtcg ggtttgcagg
      601 aaaagggcgt caaggtcggc atcatcagcc ggggctacgg gcgcaagagc aaggcggttc
      661 atgtattgaa tgctgagagc cgagcggaag atgcgggcga tgagcctttg ctgctgttcc
      721 gcaaaaccgg tgcgccgacg gcggtgggca gcagccgtgc agaggcaggc agggcgttgc
      781 tggcggcgca tcccgacatc ggactgattg tggcggacga cggtttgcag cattacgccc
      841 tgcggcgaga tgtggaaatc gcggtgtttc cggcggcgga tacggggcgc acggatttgg
      901 atttactgcc caacggcagt ttgcgcgaac ctttgttgcg gctggattcg gtggatgcgg
      961 tcgtcgtcag cggcggcaag gcggatgcgc tgtttaggcc gtctgaaaat atgtttcaca
     1021 gccgtatcga agcgggacgg atttaccgtt tgaacaatcc gtccgaaata ctggacacag
     1081 gccgtctgaa aaatcaaacc gtcgtcgccg tggcaggtat tgccaagccg gcgcgttttt
     1141 tcgattcgtt gcggaatatg ggcattaccg tgaagcgaac cgtcgcgctg cccgaccacg
     1201 ccgacatttc ggcggcagat ttgcccgatg cggacgcggt cattattacg gagaaagatg
     1261 cggtcaaatt ttcagacggc atttgcaccg ataatgtttg ggtgttgccc gtttgtgcga
     1321 taatcgaacc tgatttggcg gcgtttgtgt tggagcggtt ggaagatgta ccgaaggccg
     1381 tctgaaagca cggtttgggc ggagtgatta cggatttgaa taagaacgcc tcgcgccgtc
     1441 attcccgcgc aggcgggaat ctaagtctcg aattttcagg aatgcctagg aggcttcaga
     1501 aatcccaaat ctccggattt ccacttggac aggaatgaga aaatcggtcg tattttttat
     1561 ctgcattaat cattcgttaa agtattgaat attaaactga aaaccttgtt attgcccttc
     1621 gccacgctgg cattgtgcac caacgctttt gccgccccgc ccagcgacgc gtcgttggcg
     1681 cgttggctgg atacgcagaa ttttgaccgg gatatagaaa aaaatatgat tgagggcttt
     1741 aatgccggat ttaaaccgta tgcggacaaa gcccttgccg aaatgccgga agcgaaaaaa
     1801 gatcaggcgg cagaagcctt taaccgttat cgtgagaatg ttttgaaaga tttgattacg
     1861 cccgaagtga aacaggctgt ccgtaatact ttattgaaga atgcccgtga gatatacacg
     1921 caagaagaaa ttgacggcat gattgccttt tacggttcgc ctgtcggtca gtccgtcgtt
     1981 gccaaaaatc cgcgcttaat caagaaatcg atgagtgaaa tagcagtatc ttggactgca
     2041 ttgtcaggga aaatcgcgcg acatcatctg cccgcgttta cggaagagtt gcggcgcatc
     2101 atctgcggcg gtaaaaatcc cgatgcgggc tgtaaacaag ccggacaggt tgggaaaagg
     2161 catcagaaat aaatgatagc cgtctgaaat attgaagagg gcatccgatt gattgaacca
     2221 tcaaacccga aagcaaccct atggaaaaaa aattcttaga catcctcgtc tgccccgtta
     2281 ccaaaggcag gctggaatat catcaggaca aacaggaatt gtggagccgt caggcgaagc
     2341 ttgcctatcc gattaaagac ggcattccct atatgctgga aaacgaagcg cgtccgttgg
     2401 gcgaagagga actcgaagca tgaccgaatt cgtcgtattg attccggcgc ggctggattc
     2461 gtcgcgcctg cccggaaaag ccttggcgga catccacggc aaaccgatgg tcgtgcgcgt
     2521 tgccgaacag gcggcaaaaa gtaaagctgt gcgcgtcgtc gttgccaccg accatcccga
     2581 tattcagacg gcctgtcagg cgcacggcgt cgaagtcgtc atgacttcaa accggcacga
     2641 aagcggcacg acgcgccttg ccgaagcctc tgccgcgctg aagctgccgc cgcatctggt
     2701 ggttgtcaac gtacagggcg acgagccgct gattgcacct gaactcatcg accgcaccgc
     2761 cgaagtactc gtcgaaaaca acgtccaaat ggcgaccgcc gcccacgaat tgcacgattt
     2821 cgacgaattg atgaatccca atgccgtcaa agttgtcctc gacaaaaacc gcaacgccat
     2881 ctacttcagc cgcgccccga ttccctatcc gcgcgatgcg atgcgtgcag gaaaacgcga
     2941 aatgccgttt gaaaccgccg tcctgcgcca tatcggcatc tacgcctacc gcgtcggctt
     3001 cctgcaacgc tacaccgaaa tgagcgtttc gccgctggaa accattgaat cgctggagca
     3061 actgcgcgtc ctgtggcacg gctacccgat tgccgtcgaa accgccaaag aagctcctgc
     3121 cgccggcgta gatacgcagg aggatttgga caggattcgc gctgtatttc agactatata
     3181 aaatagattc aaagggaaaa gatatgcagc aacatattga aaaatggcaa cacttgagcc
     3241 gggaagaaca gaaaatcctt gctgaagtat ggggtctcgt gcaaaacgac gatcaggagg
     3301 ttcactatga aatgctcaaa ttgaacgcac ccgacgaagc cagtggcgaa ttttggttca
     3361 gaatggcaga aacactcagc accctgccgc ccaaccgttc cctcgacctt agaatgaacg
     3421 gcggcaggct ggcgaccgcc gtatccatcc tttccgtcat gattgaagac aatcccgaca
     3481 taccgcagct ttgggcgcaa aaaattaccg cgctcaatta tagtggatta aatttaaacc
     3541 agtacggcgt tgcctcgcct tgtcgtacta tctgtactgt ctgcggcttc gtcgccttgt
     3601 cctgattttt gttaatccac tatatttggc acacggacac aaagcccgtg ccgacggttt
     3661 ggcacaacag cccgacaaag cggcagaagc caacgaggag gaatacctga ccaaagccct
     3721 gtcgcaaaac ctgctgtcaa cattggatgt cgcgcttgca cgttttcctg aagacgcgtg
     3781 gtttcaggaa atcaaacagg atgcacaaaa gcattttgct tgaggatgtg gcagtcagga
     3841 atatttccat tcaggaagaa aagaagtgcc tgattgggta taatcagggt aaatcttatt
     3901 ttatttcaaa agattaatat ttgctttcca tttttccttg acggtatcgg aaaagttgat
     3961 tatagttaca gcttccttag gagtaatggc tgagaggctg aaggcacttc cctgctaagg
     4021 aagcatgtgg ggtcaacctg catcgagggt tcgaatccct cttactccgc caagatgaga
     4081 agacgctgtg ttttacagcg tcttttttta cgcgctttgt tgcggaagtg ccgagggctg
     4141 tctgccgtgc gccgcctgga tttgcaatgt gcccgcccgt gcaaaatccg atttgaccaa
     4201 cctgaagggc aggtttcgga agagcgggga attttgataa acaaaaaact gcacggcata
     4261 atctttcccg gttttcattt tccgtattcg cctatccgta ccgcaaatgc tatactggga
     4321 aaaatttacc gattgtgttt tacggcatat ttgccgatag gatggaagag acaaatgagc
     4381 agaatccggc aggcttttgc cgctttaaac ggtgaaaagg cattgattcc ttatattacg
     4441 gttggagacc cggatattgg ggcgactttg gcaataatgc acagtatggt tgcaaacggt
     4501 gcggatattt tagagttggg cgtgccgttt tccgatccga tggcggacgg gccggtgatt
     4561 cagcgtgcgg cggagcgggc gttggcaaac gggatttcgc tgcgcgatgt cttggatgtc
     4621 gtcagaaaat tccgtgaaac cgacacgcaa acgccggttg ttttgatggg atatttgaac
     4681 cctgtacata agatgggtta tcgggagttt gctcaggaag ccgcaaaggc gggtgtggac
     4741 ggcgtgttga cggtggattc ccctgtcgaa accatcgatc cgctctatcg cgagctgaag
     4801 gataacgggg tcgactgtat tttcctgatt gcgccgacga cgacggaaga ccgtattaaa
     4861 accattgccg agctggcagg cggatttgtc tattatgttt cgctcaaggg cgtaacgggc
     4921 gcggcaagtt tggatacgga tgaggtttcg cgtaaaatag agtatttgca tcagtatatc
     4981 gatattccca tcggtgtcgg tttcggcatc agcaatgcgg aaagtgcgcg caaaatcggc
     5041 cgggttgccg acgcggtcat tgtcggcagc cggattgtga aagagattga aaacaatgcg
     5101 ggcaacgagg ctgcggccgt cggtgctttg gtcaaagagt tgaaggatgc cgtgcgctga
     5161 ttctgtcacg cattctaaat attttaggag ttgtccatga gctggttaga taaaatcctg
     5221 ccacccaaaa tcaaaaaccg cagcaaagac ggttcttcca atgttcccga gggtttgtgg
     5281 cacaaatgtc cgtcttgttc ggcaaccgtt tattcgaccg agttgcagca gaacaatcag
     5341 gtttgtccga aatgcaacca ccacaatccg ttgtcggcac gacaacgcct gaatctgctt
     5401 ttggatgagg atggcagaga ggaggttgcc ggaaatgtca aaccgacaga tcctttgaag
     5461 tttaaagacg gcaaaaaata tccggatcgt ttgagtgcgg cacgcaagct gaccggggaa
     5521 gatgatgctt tggtggtaat gaaaggcaag atgaacggtc tgcccgttgt ggttgctgcg
     5581 tttgagttcc gctttatcgg cggttcgatg ggttcggttg tgggcgaacg attcgtacaa
     5641 ggtatccgtc gggcggttgc cgacaattgt ccgttcgtct gtgtggcggc ttccggcggt
     5701 gcgcgtatgc aggagggtgt gaactcattg atgcagatga cgaaaaccag tgccgcgctg
     5761 catttgctga cggaaaaacg cctgccgttt atatcggtgt tgaccgatcc gactatgggc
     5821 ggcgtatccg ccagcttcgc atttttgggt gatgtcgtat tggctgaacc caatgcccta
     5881 atcggttttg cgggaccgcg cgttattgaa cagactgtac gggaaacctt gccggaaggc
     5941 ttccagcgtg ctgagttttt actggagaaa ggcgcaatcg accagattgt tgaccggcgt
     6001 gagatgaagc ggcgcatcag tgatttgatt acgctgttgt gccgtcagga caaagtttcc
     6061 gccgcttgat ggctgatgaa tcgaataccg tctgaaaccg atgtttcaga cggtattttt
     6121 gtgtctggtt atttgttgtg cggctttatc gacggggcat agcgtccggc acgttctttc
     6181 aggcgttgta ccaaaccttt cgtgtcggcg ggtacatcgc cctcgcagaa tgcctgatac
     6241 aggacggtgc gcagtgcgtc gttgcggctt aatgtaccgc ctatcggttt ccattcggcg
     6301 tttcggggct gtatccagcg gcggttgacc gtgtcgccgt agccgaacac tttataggag
     6361 gaaggtttgc cgttgccgaa cctgatggag aagcgggcgc agaatatgcc ttcggcagtc
     6421 aggttgtcgt agcctttgtc ggagcggata ttgagaatgt agcggatgct gccgtcgggc
     6481 gcgggcataa tttgcaggct gtcgagcagg attttcggct gtttgccgta attttcatcc
     6541 acataaatgt cgaaccagcc gtccgagtgc gtatcgggca gcggcggcag tttggcggta
     6601 tgttctttaa attcgcgggc ggcggcctct tcgggcgttt cgcggtagcg ggtgttgatc
     6661 ggcgtgtctt tttggctgaa gccggcagcg agggacgtgc cggcggcgag tgcgagcagc
     6721 aggagggcgg tgcggcgcat aagtttctcc aaattgaaaa cggcgttatt ttatgggttg
     6781 gcaaaggggg ctgcaagcaa ctggggtata atctcccccc gattcccatt ttttaaacgg
     6841 tacaaacgat gaacagcgaa actttagacg taaccggatt gaaatgtccc ctgccgattt
     6901 tgcgggcgaa aaaggctttg gcgcaaatgc agcagggcga cgtgttgacc gttctggcaa
     6961 ccgacggcgg cgcaccgggg gattttgagg ctttttgccg ccaaaccggt catgtgctgt
     7021 tggatgcttc cgaacaggac ggcgtgttca cgctggtcgt ccaacacaaa taaatgccgt
     7081 ctgaaatgcg gatgtcccgc ccgacggcgt tgtttttgaa tatcgtatgt gccgcgtgcc
     7141 gtttcaaaaa caaaccgtcc ggcgtgcagt gtcggcattt cgggcgtatc gccgccgatt
     7201 tgttccggaa tggctgttaa ccgccttgcc gtcggcaaag aagcaaaacc caagcacaat
     7261 caaaatctaa aggctgtgtt tgaagattcc gttgatgcaa cccaatcagt cggacaaaat
     7321 gcctttcatc caatggaacc ggtttccgac cggacggaat aaccggcttt cccccggcaa
     7381 acggatggaa tcgaccgggt attcaaacgc agccaaaacc taaaaaggaa caaccatgca
     7441 aaccctgacc attatccgcc ccgacgatat gcacctgcac ctgcgcgacg gcgacgcgct
     7501 caaagccgtt gccccttata ccgcccgcca aatggggcgc gccgtcatta tgcccaacct
     7561 caaaccgcct gtcgtcagtg tagccgacgc gcttgcctac aaagcgcgca ttatggcggc
     7621 gttgcccgaa ggtagcgcgt ttgagccgtt gatgacgctt tatttgactg ataacgccac
     7681 gcccgaactt gtacgcgaag ccaaagccgc cggcatcgtc gccttcaaac tctaccctgc
     7741 cggcgcgacc accaattccg attccggcgt aaccgacctg ttcaagctca tccccgtgtt
     7801 ggaagaaatg gcgaaacagg gcattttgtt cctcgttcac ggcgaagtaa ccgaccccga
     7861 aatcgacatc ttcgaccgcg aagccgcctt tatcgggcgc gtgatgaaac ccgttttggc
     7921 gcaagtgccg aatcttaaag tcgtgttcga acacatcacc accgccgaag ccgcccgcct
     7981 ggttttggaa gcaggcgaca acgtagccgc caccgttacc ccgcaacacc tcctgctcaa
     8041 ccgcaacgac ctcttggtcg gcggcgtgcg cccccatcat ttctgcctgc ccgtactcaa
     8101 acgcgaaacc caccgtcagg cattggtcgc cgccgttacc ggcgagaagg cgcataaatt
     8161 cttcctcggc accgactccg cgccgcacgc caaatccgcc aaagaaaacg cctgcggctg
     8221 cgccggtatg ttcagtgcga tgaccgctat cgagctttac gccgaagtat ttgaaaaagc
     8281 aggcgcgttg gacaaactcg aagccttcgc ctcaaaaaac ggcgcaaggt tctacggcat
     8341 tcctgaaaat accgacacga tcaccctcgt caaacaaagc caaaccgttc ccgcaagtgt
     8401 tccctacggc gacggcgaac ttgtcccgat gcgcgcgggc ggcgaaatcg gctggacggt
     8461 gcagtattga tgggctggaa acaaaatgcc gtctgaaggg agggctttca gacggcattt
     8521 gtgttgctga ctgattgata cgtcaacggc gtttgagttt gttacgtttc ggttatttcc
     8581 gataaattcc cacaattttc aaatttcgcc attcccacga aggcaggaat ccagaaattc
     8641 gatgcgacca gagtttatca aaaacggcag caactcaaaa aaccggattc ccgcctgcgc
     8701 gggaatgacg agattgaagt ttcagaattt atttgaaata cccaaaattc aaaaaaccaa
     8761 attcccacct gcgtgggaat gacgaaacaa agaaagcaga aataaggaca tagaactttc
     8821 tttaaatttg tgatgcatca acggcgtttg ggctcgtcgg ggcggatttg ggcggcgagt
     8881 ttgtcgagga tgccgttgac gaatttgtgc ccgtccgtac cgccgaaggt tttggtaact
     8941 tcgatggctt cgttgataat gacggggtag ggcgtttcgg gcatggcgga cagctcgtgg
     9001 caggcggtca gcaggacggc gcgttcgatg gggttgaggt ctttttcgtc cctgtcgagc
     9061 agcgggcgga tttgccggat gtattctgcc gcattggttt gcgtgccgaa gaaaagtttg
     9121 ttgaacaatt cttcgtccgc tttggcaaag tcggacattt cgcggatgtt tttagcaatc
     9181 tcgggtgctg cggtgcggtt gataagtgat tggtaaacgg cttgtacggc aagctcgcgg
     9241 gaacggcggc gggctgtttt cataattttt ccttgaaacg gttgggcggc acggtatgcc
     9301 gtctgaaacg gaaagggcgt atcggtgtac gccctgtttg ttattcttcg tcttcaaact
     9361 gttcttcgag cagcaggttg acgaggttgg cgcattctac ggcgactttg gcggcatccg
     9421 aggctttttc ttcaatccgt tcgattgcct gcgcgtcgtt ttcggtggtc aggacggcat
     9481 tggcaatcgg aatgttgtag tcgagtgcga cgcggctgac ccctgctccg gattcgttgg
     9541 aaaccagctc gaaatggtag gtttcgccac ggatgacgac gccgatggca atcagtgcgt
     9601 caaacttttc ggaagaggca aggttcatca gcgcgatggg gatttcaagt gcgccgggta
     9661 cggtggcgac ggtaatgttt tcgtctgcca cgcccaattc ttggagggtg cggcagcaga
     9721 ctttgagcat ttcgctgccg atttcgttgg tgaaacgtgc ctgtacgatg ccgatgcgga
     9781 ggtgtttgcc gtcgaggttg ggggcgatgg tgttcattgg gtgtcctttg gtattcggag
     9841 gtttcggaat gccgtctgaa gggtcagtcc ttaggttgcc agtcggcaac ggtttggaat
     9901 gtgccgtctt ctgccaattc ccacgcgctg ccttcgggtt gggaaagcag tgcggcggtt
     9961 tcagggttgg ttttggcgat gttggcgagg ctgacgatac tgaagttgtc ggggtcgtcg
    10021 gtgtattcgt cggtctcgtc gccgctgaag aaacgccagc cgctgtcgtt ttcaaaaacg
    10081 ggggcttcgc ggtagaggaa gccgacaggc tggttttgtt tggcgacggt gttggtggcg
    10141 atgcagcggt cgagtgccga ggaaagtgct tgtgcaaatg cgttcattgc gggaatacgt
    10201 tgggggaaaa cttacggatt ttaccacgat tcttgcgttg tcggcagacg gcggcggttt
    10261 ggtggtacaa tgtgcgccgt ttgcagcctt aaggtgtttc tgtattttta gagtatggaa
    10321 acgcattcgg gctgtttttt gcggaagacg gtaatgaaag acgatgtttt gaaacagcag
    10381 gcacacgcgg cgatacagaa gaaactgggc tatgcgttcc gcgatatttc gcttttgcgg
    10441 caggctttga cgcacaggag ctgccatacg aaacataatg aacggtttga gtttgtcggc
    10501 gattcgattt tgaattatac ggtggcgcgg atgctgtttg acgcgtttcc gaagttgacc
    10561 gagggcgagt tgtcgcggtt gagggctagt ctggtcaatg agggcgtgct ggcggaaatg
    10621 gcggcggaaa tgaatgtcgg cgacggtctg tacttggggg cgggcgagtt gaagagcggc
    10681 ggcttcagac ggccttcgat actggcggac gcgatggagg cgatgtttgc ggcggtcagc
    10741 ttcgatgccg atttcaacac ggcggaaaag gtggtgcgcc atttgtttgc cgatcgcgtc
    10801 cggtgtgctg atttccaaaa tcaggcaaaa gacggcaaaa ctgctttgca ggaggcgttg
    10861 caggcgcgtc gtttcgcttt gccgaaatac cgtatcgaag agcaaatcgg ccatgccaac
    10921 gacagtatgt ttgtggtttc ctgcgatttg ggtgaactgg gtttcgtgtg ccgtgccaaa
    10981 gggacgagcc gcaaggcggc ggagcaggaa gcggcgaaag aggctttgaa atggctggaa
    11041 gagaagctgc cgctgaagaa gaaaaagaaa tgaggcggcg cgtgaatatg ccgtctgaac
    11101 atatagatac gaaagcaaat atggatattg aaaccttcct tgcaggggaa cgcgccgccg
    11161 gcggataccg ttgcggcttc gtagcgattg tcggccgtcc gaatgtcggc aaatcaacgc
    11221 tgatgaacca tctcatcggt cagaaaatca gtattaccag caaaaaggcg cagacgacgc
    11281 gcaaccgcgt aacggggatt tataccgacg ataccgcgca gttcgtgttt gtcgatacgc
    11341 cgggttttca aaccgaccac cgcaacgcgc tcaacgaccg tttgaaccaa aacgttaccg
    11401 aggcactcgg cggtgtggat gtggtggttt tcgtcgtgga agcgatgcgt tttaccgatg
    11461 ccgaccgcgt cgtgttgaaa caactgccca agcacacgcc tgtggttttg gtggtcaaca
    11521 aaatcgataa ggacaaggcg aaagaccgtt acgcgctgga ggcgtttgtt gcccaagtgc
    11581 gcgccgaatt tgaatttgcg gcggcggagg cggtcagcgc gaaacacgga ttgcggattg
    11641 ccaacctgtt ggagctgatt aagccgtatc tgcccgaaag cgtgccgatg tatcccgaag
    11701 atatggttac ggacaaatcg gcgcgttttt tggcgatgga aatcgtgcgt gaaaaattgt
    11761 tccgctattt gggcgaggaa ttgccttatg cgatgaacgt cgaagtggag cagtttgaag
    11821 aggaagacgg tttaaaccgc atctatatcg ccgttttggt cgataaggaa agccaaaagg
    11881 caattttaat cggtaaaggc ggagaacgtt tgaagaaaat ttccaccgaa gcgcggttgg
    11941 atatggaaaa actgtttgat accaaagtat ttttgaaggt ctgggtcaaa gtcaaatccg
    12001 gttgggcgga cgacatccgc ttcctgcgcg agctgggttt gtagtttttc ttgctgaact
    12061 ttacgcaaat gccgtccgaa caggtttcag acggcatttt gtttcaatcg ggaatatctt
    12121 tgttaaaaac gggttgatat tatctgtgca
//
LOCUS       DAXHRI010000046        12010 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXHRI010000046 DAXHRI010000000
VERSION     DAXHRI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12010
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.046"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            138..567
                     /locus_tag="ACFM75_000955"
                     /pseudo
     CDS             138..567
                     /locus_tag="ACFM75_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
     gene            complement(615..1958)
                     /locus_tag="ACFM75_000956"
     CDS             complement(615..1958)
                     /locus_tag="ACFM75_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC6255528.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTDRA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1963..2604)
                     /locus_tag="ACFM75_000957"
     CDS             complement(1963..2604)
                     /locus_tag="ACFM75_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC6255529.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSRKVARTAAV
                     AVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNLGAQP
                     ETGQVRPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGLVVSA
                     ICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2755..3045
                     /gene="gatC"
                     /locus_tag="ACFM75_000958"
     CDS             2755..3045
                     /gene="gatC"
                     /locus_tag="ACFM75_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC6255530.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3108..4553
                     /gene="gatA"
                     /locus_tag="ACFM75_000959"
     CDS             3108..4553
                     /gene="gatA"
                     /locus_tag="ACFM75_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC6255531.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVQTA
                     LQNTINLLKTQGAELVEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4550..5482
                     /locus_tag="ACFM75_000960"
     CDS             4550..5482
                     /locus_tag="ACFM75_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC6255532.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5525..6955
                     /gene="gatB"
                     /locus_tag="ACFM75_000961"
     CDS             5525..6955
                     /gene="gatB"
                     /locus_tag="ACFM75_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC6255533.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINITRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPELR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKAGLELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPESTIAEIIEKHGLQQMTDTGAVEAIVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7047..8057
                     /locus_tag="ACFM75_000962"
     CDS             7047..8057
                     /locus_tag="ACFM75_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC6255534.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIENIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRIKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8186..8818)
                     /gene="pdxH"
                     /locus_tag="ACFM75_000963"
     CDS             complement(8186..8818)
                     /gene="pdxH"
                     /locus_tag="ACFM75_000963"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC6255535.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9049..10098
                     /locus_tag="ACFM75_000964"
     CDS             9049..10098
                     /locus_tag="ACFM75_000964"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6255536.1"
                     /translation="MSKQPTSKRQWRDGVVPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGERRRKKA"
     gene            complement(10167..11708)
                     /locus_tag="ACFM75_000965"
     CDS             complement(10167..11708)
                     /locus_tag="ACFM75_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC6255537.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQASVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
BASE COUNT         3086 a         3651 c         2966 g         2307 t
ORIGIN      
        1 gggaatctgt tttaccccaa tatataaagc accatattaa ggcggagttt cttccccact
       61 ttgaaccgaa cccggaaaag acaccgccca agccaatcct gatgctgccc cggcagccaa
      121 ccatcaagga aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt
      181 tcctgcccgt cccgcctgcc ggagccgccg tctttacttg gaaggacggc ggcggcaaca
      241 gctattcgga tgtaccgaaa cagcttcatc ccgaccaaag ccaaatctta aacctgcgga
      301 cgcgccaaac caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg
      361 gcgcggcaca ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa
      421 agaattgccg aaaccgaacg gcagaacaaa gaagaaaact gccggatttc aaaaatgaac
      481 ctgaaggcgg tgggaaattc aaatgcaaaa aacaaggatg atttgattcg gaaatacaat
      541 aacgccgtaa acaaatactg ccgttaatcg gctctagcgc aaacccgatg ccgtctgaag
      601 cggcacgggg tttgtcattt ctgccagtag gttttgacgt tgacgaactc gtacagcccg
      661 aattcggaca attcgcgccc gtaaccggaa tctttgactc cgccgaaagg caggcgcaaa
      721 tcgctgctgg tatggcggtt gataaacacc gatcccgcct gtattttttc ggcaaaccgc
      781 catgcccggt cagtatcggc ggtataaatg caggcgccga gtccgaacgg tgaatcattg
      841 gcaagggcga tggcatgttc ttcgttttcg gcgcgcaaaa tcagggcggc cggcccgaat
      901 acttcttctc tccagacgcg gcaggcagga tttaccctgt ctaaaaccgt cgcgggataa
      961 aaccagcctc gcccttgtgg gatttttccg ccggtcaggc ataccgcgcc gtttgaaacg
     1021 gcatcttcaa cctgcccgtg aaccctgtcc cgcaaatctt cgcggtgcag cggtgcaagc
     1081 gtagtatcgg gatgtttggg gtcgcccatt ttcaatttag cgcattcggc aagaaacagc
     1141 gtgataaaac gatcggctgc ggcttcggtt acgatgatgc gcttggcggc gttacacgat
     1201 tgccccgcat cgcggaaacg ggaataacag gcttctgcgg cggcacgctc caaatcagca
     1261 tcgggcatca cgataaaggc gttgctaccg ccgagctcca acacggtttt cttaaggttt
     1321 gcgcccgcgt gtgccgcaag gatgcgcccc gtatgcgttg aaccggtaaa cgccattgca
     1381 tcggtatctt caaccgcctt gagcgtgccc gcctcatcca gccacacgcc tgccagagga
     1441 atgccgtctg aagccaaatc gaacagtgcc tgactgacgc gtgccacgct gggcgcgggt
     1501 ttgacggcgc acgcgttgcc cgcgcacata gcgggaacgg cgaaacgcaa tacctgccag
     1561 acgggatagt tccaaggcat gacggcaaac accacgccca aaggctcgaa gcgcacctga
     1621 ctcaaactcg cctgcgtcgc gatggttttg tgggcaagca gttcgggggc aaggcgggcg
     1681 taatagcgta tcagttcgat agacttgccg atttccgcac ggcattcgtg caagcagcgt
     1741 ccgacttcct cacacaccat ttccgcaaaa cgctctttct ccgcctccaa acggtcggca
     1801 aatttttgca ggcgcgcggc acgttcggtt acgcccagtt gcgcgaacgc cccgccgcgc
     1861 attttcaaat ccgccagccg ccgttcaaac tccgcataat cttgagctgg acggcggtaa
     1921 agcgtttcgc ccgtaaatac attgacactg tgaaacatcg aatcaacctg ccagttgcgg
     1981 gaatatcgtt ttcagtcccg acacaataat ctccaccgat accgccgcca gcatcatacc
     2041 catgatacgg tttaaaatcg tcagccccgt cgcgcccagc aggcggctga ccttcccggc
     2101 aacgattaaa atggcataac aaatcgcact gaccaccaaa ccggccacga taatcagcgc
     2161 gatgtcgctg tacgttttgg ctgccgaagc gtaaataatc acggtcgaaa taccgcccgg
     2221 gccgatggtg atcggtatgg cgatgggcac gacggcaatc gctccggcat tgcgggcggg
     2281 gcgcacctgc cccgtttccg gctgcgcgcc gagattctgc ttggcgggat tgtcgttgcc
     2341 gttcatcatc gaaatggcaa tcagcaacac caaaattccg ccgccgacct gaaacgaacc
     2401 gacgctgatg cccaaaacct tcagcagcgt accgccgatc agcgcaaata ccgcaatcac
     2461 ggcaaacacg gcaacggcgg ccgtccgcgc gaccttcctg ctgtgcccgt tggtcaggtc
     2521 aaggtaaagc gacaacgcgc taaacggatt aatcagcacc aaaaaagcca caatcagctt
     2581 gccgatttcc atgcccaatc ccattatttc cccctccttc aaacccgtgc ggcaggcatc
     2641 cgatgctgca aattgccgcc gcaacggatt tttccgttat aattaaaaat tcaagcaata
     2701 cgccccatca tacccgaacg acggtatctt taccatcaga caaggatgct tttcatggca
     2761 ctgacacttg ccgacgtaga caaaatcgcc cgactctccc gactgcacct gactgcggaa
     2821 gaaaaagaaa aatcgcttca agaattaaac gacattttca ctatggtcga acagatgcaa
     2881 accatcaaca cagacggcat cgaaccgatg gcgcacccgc acgaggccgc cctgcgcctg
     2941 cgcgaagacg aagtaaccga aaccgaccgc gccgccgaat atcaggcggg tgctccggaa
     3001 gtacgcaacc gtctgtacat cgtaccgcaa gttatcgaag aataatccga atatgcttca
     3061 gacggcatca gcaataccgc ccgaagccct ttaaggatgg aagatttatg acccaataca
     3121 cattgaaaca ggcaagcgtc ctgttgcagt ccaaacagat ttccgccgtc gaactggcaa
     3181 gcgcatacct tgccgccatc gccgaaaaaa atcccgccct caacggctat atcaccatcg
     3241 accaagataa aacccttgca gaagcccgtg ccgccgacga acgtatcgcg cagggcaacg
     3301 cctccgcgct taccggcgta cccgtcgcct acaaggatat tttctgccaa accggctggc
     3361 gcagcgcgtg cgcttccaaa atgctcgaca acttcatctc cccctacacc gccaccgtcg
     3421 tccaaaacct gctcgacgaa ggtatggtaa cgctcggccg caccaatatg gatgagttcg
     3481 ctatgggttc gaccaatgaa aactcattct acggtgcagc caaaaaccca tggaatcttg
     3541 agcacgtccc cggcggttcg tcaggcggtt ccgccgccgt cgttgccgcg cgcctcgccc
     3601 ctgccgcgct cggttcggac accggcggct ctatccgcca gcctgcatcg cactgcggca
     3661 ttaccggcat caaacccaca tacggcacgg tttcccgctt cggtatggtc gcctacgcct
     3721 ccagcttcga ccaggccggt ccgatggcgc aaaccgccga agactgcgcg attctgttga
     3781 acgcgatggc aggtttcgac cccaaagact ccaccagcct cgaacgcgaa aaagaagact
     3841 acacccgcga tttgaaccaa ccgctcaaag gcgtgaaaat cggcctgccc aaagaatatt
     3901 tcggcgaagg caacagcgcc gatgttcaga cggcattgca aaatactatc aacctcctca
     3961 aaacccaagg cgcggaattg gtcgaagttt ccctgccgca aaccaagctg tccatccccg
     4021 cctactacgt cctcgcctcc gcagaagcca gtaccaacct ttcacgttac gacggcgtac
     4081 gttacggaca ccgtgccgcc caattcggcg atttggaaga aatgtacggc aaaacccgcg
     4141 ccgaaggttt cggcagcgaa gttaaacgcc gcatcatgat cggcacttat gtactgtcac
     4201 acggctacta cgatgcctac tatctcaaag cccaaaaact gcgccgcctc gttgccgatg
     4261 attttcagac ggcatttgca cggtgcgacc tcatcctcgc gccgaccgca cccactgcag
     4321 ccccaaaaat cggagcggat gcttcgccgg ttgaaaccta cttgagcgat atctacacca
     4381 tcgccgtcaa cctcgccgga ctgcccgcat tgaccctgcc cgcaggcttc agcggcggcg
     4441 gactgcccgt cggcgttcag cttgtcggca actacttcgc cgaagccaaa atcctcggtg
     4501 cggcgcatca aatccaactc aacagcgatt ggcacggcaa acgacccgaa tgaagcagaa
     4561 ccgcaccttt accttccccg attttcgcac cgtttacagc tatgcgcctt tatatcggct
     4621 gcaacattta aaatacacat tgcgaaaatt tttcggaaaa aaagaaattt acgccttcga
     4681 gcagtttgtc aacgcatccc ctatccgtca ggggctgttc ctccactgcc cgcaaaatgc
     4741 ctatccgctg ctgcgcgaat ttgttgacag gcgttttaac tgcaaacgcc gtttagatgc
     4801 gatgacggca gattttctca tggcggaaaa actgttcggc acagacatcc tgcaccaaat
     4861 ggaagactac cgcttccatt tggtcttggc gcacctttca gacggcatca gcttgtggct
     4921 caaccgcaac gacaactgcg tcgaagaagg cgcgtggtct ttatctttgc gcgacgaagc
     4981 aggcaaccgg ctgtatatgg cgaccttcgc ctttgtcggc acacacctgc tgacagcctc
     5041 cgtacaaggg ccggcgggtg aagaagccaa agacaccgtc cgccgcataa ccaaacaact
     5101 ccacggcttg cgtccccaac aactgatggt aaccgccctg caatatttcg ccgccgtact
     5161 cggcttggac ggcgcaatgg gcattgcaca aaaacatcag gtcaaactgc gctggaaact
     5221 taaaaagcgt gtcaaaatga attacgacgc attctggcag gaatacggcg caagtttgga
     5281 acgggacggc tactggcatc tcccccaaac ccccgcccgc aaagacctta ccgacatcga
     5341 aagcaaaaaa cgctcgatgt accgcaagcg ttatgaaatg ctggacaata tggttgcaga
     5401 gatgaaggac ggtctgaaaa cagaagcacg cggcatttca gacggcatcc aaacggaaaa
     5461 accgctccgc cggacagcct gacgcgaaga ctatcgaatt gatattttag agaaagaagc
     5521 tcttatgacc tgggaaaccg taatcggctt ggaaatccac gtccaattga acaccaaatc
     5581 caaaatcttc agcggcgcat cgaccgcatt cggcgcagaa cccaacgcgc acgccggcgt
     5641 agtggaatgt gctttgccgg gcgtactgcc ggtaatgaac cgagaagtcg ttgaaaaagc
     5701 catcaaatta ggcttggctt tggatgcgaa aatcaaccgg aaaaacgtgt tcgaccgtaa
     5761 aaactacttc tatcccgact taccaaaagg ctatcaaatc agccagttgg acttaccgat
     5821 tgtcgaacac ggcaaattgg aaatcgtagt cggcgacgat gtgaaaacca tcaacataac
     5881 ccgcgcccat atggaagaag acgcaggtaa gtccgtgcat gaaggcttga acggcgcgac
     5941 cggtatcgac ctgaatcgcg ccggcacacc tttattggaa gtcgtatccg aaccggaatt
     6001 gcgctccgcc gccgaagccg ttgcctacgc caaagccttg cacagcttgg taacctggct
     6061 ggacatttgc gacggcaata tggcggaagg ttcgttccgc gtcgatgcca acgtatccgt
     6121 gcgcccgaaa ggtcaagaag agttcggcac gcgccgcgag attaaaaacc tcaattcctt
     6181 ccgtttcttg gagcaggcga ttaattacga agcagaagcg caaatcgaga ttttggaaga
     6241 cggcggcaaa gtacagcagg caaccatgct gtttgatccc gaaaaaggcg aaacccgcgt
     6301 aatgcgcctg aaagaagacg cgcaagacta ccgctacttc cccgaccccg atttgctgcc
     6361 cgtcatcatt tcagacgctc aaatgcaaaa agccaaagca gaaatgcccg agttgccgaa
     6421 agaaatggca gcgcgtttcg tggcggacta cggcgtgtcc gaatacgacg cgcgcctgct
     6481 caccgcaagc cgcgcacagg ccgcctattt tgaagaagcc gccaaagaga gcggacaagg
     6541 caagccgact gccaactgga tgaacggcga actcgccgcc gcgctgaaca aagccggcct
     6601 ggaacttgcc gacagcccga ttaccgcccc gcgcctcgct gcgctggtgg gcaaaatcgc
     6661 cgatggcaca ttaagcggca aactggcgaa aaaagccttt gaggccatgt gggcagaacc
     6721 cgaatccacc attgccgaaa tcattgaaaa acacggcttg cagcaaatga ccgataccgg
     6781 cgcagttgaa gccattgtgg acgaagtgct ggcaaacaac gccaaagccg tggaacaatt
     6841 caaatccggc aacgaaaaag ccctgaatgc gattgtgggg caagtgatga aggccagcaa
     6901 aggcaaagcc aatcccgcgc aggttcaaga actgattaaa gccaaacttg cctgacaagc
     6961 aagatgccgt ctgaaaccca gatccgacgt tcagacggca ttttcacagt ctgaccacat
     7021 taaaacagca taaaaaacaa tattccatga accacaccgt taccctgccc gaccaaacca
     7081 cctttgccgc caacgacggc gaaaccgttt tggccgctgc cgcccgtcaa aacctcaatc
     7141 tgccccattc ctgcaaaagc ggcgtatgcg ggcaatgcaa agccgaactg gtcagcggcg
     7201 atattcaaat gggcggacac tcggaacagg ctttatccga agcagaaaaa gcgcaaggca
     7261 agattttgat gtgctgcacc actgcgcaaa gcgatatcag catcaacatc cccggctaca
     7321 aagccgatgc cctacccgtc cgcactctgc ccgcacgcat cgaaaatatt attttcaaac
     7381 acgatgtcgc cctcctgaaa cttgccctgc ccaaagcccc gccgtttgcc ttctacgctg
     7441 ggcaatacat tgatttactg ctgccgggca acgtcagccg cagctactcc atcgccaatc
     7501 cgcccgacca agaaggcatt ttggaactgc acatccgcag gcgcgaaaac ggtgtctgtt
     7561 cggaaatgat tttcggcagc gaacccaaag tcaaagaaaa aggcatcgtc cgcattaaag
     7621 gcccgctcgg ttcgtttacc ttgcaggaag acagcggcaa acccgttatc ctgctggcaa
     7681 ccggcacagg ctacgccccc atccgcagca tcctgctcga ccttatccgc caagacagcg
     7741 gccgcgccgt ccatttctac tggggcgcgc gtcatcaaga tgatttgtat gccctcgaag
     7801 aagcacaagg gttggcatgc cgtctgaaaa acgcctgctt cacccccgta ttgtcccgcc
     7861 ccggagaggg ctggcaggga agaaaaggtc acgtacaaga catcgcggca caagaccatc
     7921 ccgacctgtc ggaatacgaa gtatttgcct gcggctctcc ggctatgacc gaacaagcaa
     7981 agaatctgtt tgtgcaacag cataagctgc cggaaaactt gtttttctcc gacgcattca
     8041 cgccgtccgc atcataattc cccggtataa aaaagattca ggctttccgt tcggaacaca
     8101 aaaaacttcc cgtccgtatt ttccccataa aaaatgccgt ctgaagcccg attccggttt
     8161 tcagacggca tatgtttttt cctgttcaag gcgacagccg ctcgcgtatc cagccaccat
     8221 ccagcaaacg gtattggatg cggtcgtgca gcctgctcgg cctgccctgc caaaactcaa
     8281 tcaaatcggg aatcacaata tagccgcccc aatgcggcgg acgcggcacg tgcaaaggat
     8341 gtttgagtcc gaccgccgcc gcctttgcca ccaataccgc cttgttcgga atcacctcgc
     8401 tctgcgcact tgcccacgca cccaaacggc tctgatacgg gcggctctca aaatattcgt
     8461 ccgacaactt ctccgccagc ctttcaacac gcccttccac gcgcacctga cgctccagct
     8521 ccggccaaaa aaacgtcatc gccgcaaatg gatgagcatc cagcgaacgt cccttacggc
     8581 tgtgataatt cgtaaagaaa acaaaacctt cagaattaac ttccttcagc agcaccatac
     8641 ggctgttggg tctgccgcgt ccgtcgaccg ccgccacatt aaccgccgtc ggctcgttga
     8701 cctgtgcgcg taccgcctcg tccaaccacc gctcgaactg ctcgatcgga ttgtcggcgc
     8761 aatcgccttc cgacaattcc cgtttactgt aatcttcccg aatattgtgc aaatccattt
     8821 accgctcctc ttgtcatttg aaagattcta cccccgcacg caaaccgatt tcaaccgttg
     8881 cacaaacttt gcctcgcttc tccaaacctc aggacacaac gatttcatcc gcaaaaccgc
     8941 cgcataaggt acaatatcga accgtccgac cgagggcggc attttatcaa cccgtcctgc
     9001 cgcacacgcc gcagaagaac cgccttatca ggcgagttag gaaaaatgat gtccaaacag
     9061 cccaccagca aacgccaatg gcgcgacggc gtagtcccgt ctgccaagaa aaccgccaaa
     9121 cctttcaaaa gcaaagcccg tcccaaagac gaaacggaca aaaccgcatc ccaaccttac
     9181 ggacaaaaag cttcagacgg catcaaacct caaaacctcc ccaaacagcg cgccgccaaa
     9241 gccaaaaaac tcgtcgtccg caatcccaac caaaaaatta tggaacacgc gcgcgattta
     9301 aaagaacgcc gcagcgacct atcgcgcatg gaacccgaac gcctgcaaaa agtgcttgcc
     9361 gcgtccggcg tcggctcgcg ccgcgaaatg gaagaatgga tcaacaacgg ctgggtaacg
     9421 gtcaacggca aaaccgcgca actgggcgac aaagtcaccc ccgacgacca cgttaccgtc
     9481 aaaggcagca tcatcaaact caaatgggcc gaccgcctgc cgcgcatcat cctatattac
     9541 aaacaagaag gcgaaatcgt ttcccgcgac gacccgcaag gccgcgtcag catattcgac
     9601 cgcctgccgc aggccgccag cagccgctgg gtcgccatcg gacgcttgga catcaacacc
     9661 agcggacttc tgattctcac cacctccggc gaactcgtcc aacgtttcgc ccaccccagc
     9721 ttcgaagtcg aacgcgaata cgccgtgcgc gtactgggcg ggctgaccgg cgaacaaatg
     9781 cgcgtcctca ccgaagaagg cgtgatgctc gaagacggct tggcaaaagt cgaacgcatc
     9841 cgcgaacaag gcggcgaagg cgcgaacaaa tggtacaacg tcgtgattaa agaaggccgc
     9901 aaccgcgaag tgcgccgcat ttttgaaagc caaggactca ccgtcagccg cctcgtgcgc
     9961 gtcggcttcg gccccatcgg actgcccaac cgcctcaaac gcggacagtt ctacgaactc
    10021 aaccccgccg aagtcgccaa catcctcaaa tgggcggaaa tgctgctgcc gggcgaacgc
    10081 cgccgcaaaa aagcctaaac ccgccaaaac acaaaaatgc cgtctgaaac atctactgtt
    10141 tcagacggca ttttattcgg gcgttttcag gagaaaaggt cgagtgcttt gacaaagacc
    10201 atcaccacgc cgtaggcgag cggtatgacg gccaatgccc aacgccacca gaccgatatg
    10261 ccgccgccgg aaactttttc gcccacaagg taagcgtcgg atatggcggt ttcgtcatcg
    10321 gggttgccgc tgtgtgcggc ggttttgatg tctttttcgt ggtgtttttc gtgtacggat
    10381 ttgacggcga ggttgcacaa caaaccgata atcagcaggc acgccatgat gtacatggtt
    10441 acgctgtatg cctgtgccgc cggtatgccg ctgtcgattt ggctttggcg tatgtaattg
    10501 accagtaccg ggccgatgac ggcggcggtt gaccaggcca gcaggatgcg cccgtgaatc
    10561 gcgccgacct gataggtgcc gaacaggtct ttcaggtagg cgggaatggc ggcaaatccg
    10621 ccgccgtaca tggaaataat cacgcaaaag ccgatgatga acagggcttt gctgccgccc
    10681 tcgccgatgg agggaacggc gaaatacagc agcgaaccga gtacgaagaa aatggtgtag
    10741 gtgtttttgc gtccgatttt gtcggaaacg ctcgaccata aaaagcgtcc gcccatgtta
    10801 aacaggctca ggaggctgac gaagcctgcc gccgcacctg cgccgactga cgcctgcctg
    10861 cctacggagg tttcggaaaa gagttcctga atcatcacgg atgcctgacc caatacgcca
    10921 atgccggcgg ttacgttcag gcacaatacc cagaacaaca gccaaaattg cggcgttttc
    10981 atggcttgtg acacgttgac atgattgctg ctgaccagct tgttttgcgt tttcggcgcg
    11041 gtatagcctt caggtttcca gccgtcggca ggtacgcgga tggtaaacgc gccgaacatc
    11101 atcagtgcga ggtaaagcag gcccaatacg gcgaaggttt caacaacccc gactgaagcg
    11161 gcgtttgaaa aggcgttcat cagtgatacg gaaagcggag aggccagcat tgcgccgccg
    11221 ccgaaaccca taatcgccaa accggtcgcc atacccggct tgtcgggaaa ccatttcatc
    11281 agtgtggaaa ccggcccgat gtagcctaag cctaagccta cgccaccgat gacgccgttg
    11341 cccaaataaa gcaggaagag gttgtgcgta cgcacgccga atgcggatac gaagaagccc
    11401 aggctgaaac agcaggcggc ggcaaatatg gctttgcgcg gccctacccg ttccatccat
    11461 gtgccgaaca gggcggccga cgcgcccagc atcgcgagtg cgatactgaa aatccaacct
    11521 acggtcgtca gcttccaatc tccggccgcc gattcggtta tgccgataag tttggtcagc
    11581 ggcgcgttga atacggaata ggcgtaaatc tgcccgatgg caaggtgtac cgccaatgct
    11641 gcgggcggta cgagccaacg gttgaaaccc ggcttggcaa tgcttgcctc acggtctaaa
    11701 aacttcataa catcctcttt ctgtcagttg aaaaataaaa tttcatttgc tcaatggaaa
    11761 cttattgaaa attataaaaa aatatcgggt cgggttttta tccgccccaa gatgcgccgt
    11821 ctgaaacatt tcgggtgtac ggaaaggttt ctgttttttc cgacaaattc ctgcggcttt
    11881 tcgcttccgg attcccgctt ttgcgggaat gacggcggcg ggttgctgtt ttttgtttat
    11941 aaatttctgt aactttggat ttcagccatc ttcaataaat tatcgaaacg tatcaatcgt
    12001 cattcccgcg
//
LOCUS       DAXHRI010000047        11759 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXHRI010000047 DAXHRI010000000
VERSION     DAXHRI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.047"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            40..462
                     /locus_tag="ACFM75_000966"
     CDS             40..462
                     /locus_tag="ACFM75_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255538.1"
                     /translation="MSAVFIAPYLTAFHEQEKTFEYAELTVTAPNRSGRAIKLDADGQ
                     HYRLSCYGFDSLCTGGNIGRTIRVEQVRAVLSETVGKGFLNGVLLEYRNSGSVYSNKD
                     FARTEDRLVEVLAQPAVFCLKLGILLLLPAIFLRLKRM"
     gene            568..1131
                     /gene="pgsA"
                     /locus_tag="ACFM75_000967"
     CDS             568..1131
                     /gene="pgsA"
                     /locus_tag="ACFM75_000967"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC6255539.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1211..1286
                     /locus_tag="ACFM75_000968"
     tRNA            1211..1286
                     /locus_tag="ACFM75_000968"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1244..1246,aa:Gly,seq:gcc)
     gene            1312..1387
                     /locus_tag="ACFM75_000969"
     tRNA            1312..1387
                     /locus_tag="ACFM75_000969"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1345..1347,aa:Gly,seq:gcc)
     gene            1411..1486
                     /locus_tag="ACFM75_000970"
     tRNA            1411..1486
                     /locus_tag="ACFM75_000970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1444..1446,aa:Gly,seq:gcc)
     gene            1508..1583
                     /locus_tag="ACFM75_000971"
     tRNA            1508..1583
                     /locus_tag="ACFM75_000971"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1541..1543,aa:Gly,seq:gcc)
     gene            1609..1682
                     /locus_tag="ACFM75_000972"
     tRNA            1609..1682
                     /locus_tag="ACFM75_000972"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1641..1643,aa:Cys,seq:gca)
     gene            1785..1874
                     /locus_tag="ACFM75_000973"
     tRNA            1785..1874
                     /locus_tag="ACFM75_000973"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1819..1821,aa:Leu,seq:taa)
     gene            1978..3030
                     /gene="sohB"
                     /locus_tag="ACFM75_000974"
     CDS             1978..3030
                     /gene="sohB"
                     /locus_tag="ACFM75_000974"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC6255540.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3081..3947)
                     /locus_tag="ACFM75_000975"
     CDS             complement(3081..3947)
                     /locus_tag="ACFM75_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC6255541.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAV
                     IAGKVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLECDNHFIRKGDRRQLYRHYGLVARLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(3957..4388)
                     /locus_tag="ACFM75_000976"
     CDS             complement(3957..4388)
                     /locus_tag="ACFM75_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC6255542.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTETGKAHAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(4513..5382)
                     /locus_tag="ACFM75_000977"
     CDS             complement(4513..5382)
                     /locus_tag="ACFM75_000977"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6255543.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(5477..6394)
                     /gene="hisB"
                     /locus_tag="ACFM75_000978"
     CDS             complement(5477..6394)
                     /gene="hisB"
                     /locus_tag="ACFM75_000978"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC6255544.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPNARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(6391..7488)
                     /gene="hisC"
                     /locus_tag="ACFM75_000979"
     CDS             complement(6391..7488)
                     /gene="hisC"
                     /locus_tag="ACFM75_000979"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC6255545.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(7534..8823)
                     /gene="hisD"
                     /locus_tag="ACFM75_000980"
     CDS             complement(7534..8823)
                     /gene="hisD"
                     /locus_tag="ACFM75_000980"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC6255546.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(8820..9719)
                     /locus_tag="ACFM75_000981"
     CDS             complement(8820..9719)
                     /locus_tag="ACFM75_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC6255547.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKVLYIGKGFG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(9823..10476)
                     /gene="hisG"
                     /locus_tag="ACFM75_000982"
     CDS             complement(9823..10476)
                     /gene="hisG"
                     /locus_tag="ACFM75_000982"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC6255548.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(10555..11208)
                     /locus_tag="ACFM75_000983"
     CDS             complement(10555..11208)
                     /locus_tag="ACFM75_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC6255549.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            11484..11568
                     /locus_tag="ACFM75_000984"
     tRNA            11484..11568
                     /locus_tag="ACFM75_000984"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11518..11520,aa:Leu,seq:caa)
BASE COUNT         2615 a         2799 c         3295 g         3050 t
ORIGIN      
        1 tatcgctggg tatggtttgc cgccataagc tgtctgctgt tgtccgccgt ttttattgca
       61 ccttacctga cggcatttca cgaacaagaa aaaacatttg aatatgcgga attgaccgtt
      121 accgccccca accgcagcgg acgggcaatc aaactggatg cagacgggca gcattaccgg
      181 ctttcctgct atgggttcga cagtttgtgc acaggcggca atatcggcag aaccatcagg
      241 gtggagcagg ttagagccgt tttaagcgaa accgtcggca aaggtttttt aaacggcgtt
      301 ctgttggaat accgcaacag tggcagtgtc tatagcaata aagattttgc ccgcacggaa
      361 gaccgccttg tcgaagtgtt ggcacaacct gccgttttct gcctgaaact gggtattttg
      421 cttttattgc ccgccatttt cctgcgtttg aaaagaatgt gaaaacagat tggcggggcg
      481 ggggaatgcc catgcagcaa ttttacgttt tgggttatga ggataaccgt tataatcaca
      541 actttacagt ctgcacagag aaaaccgatg ccttggaatc tccccatctt cctcacatgg
      601 ttgagggtct tgctcattcc tgtcatgaca gccctttttt acctgccttt tccgtggttc
      661 gcggaggaaa cgattaatct caccgccgcc gtcatttttg ccgttgccgc cttaaccgac
      721 tggtttgacg gatttttggc aagactgtgg aaacagactt ccgatttcgg cgccttcctc
      781 gatcccgtcg ccgacaagct gatggttgcc gtatcgctac ttttactggt caaactcgac
      841 cggacctatg ttttgtttgc catgatcatt atcggcaggg aaattaccat ttccgcattg
      901 cgcgaatgga tggcgcaaat gggcaaaagg aacagcgttg ccgtcgccac cgtcggtaag
      961 tttaaaaccg ccgcgcaaat gctggcaatc ttcctgctgc tgctgaatat tcccgatttt
     1021 tacggattta atcttgttgt tatcggcaat gtattgatgt ttatcgcatc tttgctgaca
     1081 gtctggtcga tgctgtatta tctgaaaatg gcgtggaaag aaatcgcctg aaaaaaacat
     1141 aaaaatagct tgacggcaaa aacagaatcc ataataattg cgtctcttcg atgttggaga
     1201 atgaaatcgg gcgggaatag ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc
     1261 gagttcgaga ctcgtttccc gctccaaaaa tttcattttc ccacaatcat tgcgggaata
     1321 gctcagttgg tagagcgcaa ccttgccaag gttgaggtcg cgagttcgag actcgtttcc
     1381 cgctccaagt tttatttcaa acatatcaac gcgggaatag ctcagttggt agagcgcaac
     1441 cttgccaagg ttgaggtcgc gagttcgaga ctcgttttcc gctccaaaaa ccgatatgtt
     1501 tgaacgcgcg ggaatagctc agttggtaga gcgcaacctt gccaaggttg aggtcgcgag
     1561 ttcgagactc gtttcccgct ccaagttttt tagataagcc agttttaggg cgagatagca
     1621 aagtggttat gcagcggatt gcaaatccgt ctacgccggt tcgattccga ctctcgcctc
     1681 cattatcgta ctatggcggg gtggcagagt gtttatgcta tgaggagtgc aatcttcata
     1741 taggccggtt aaaatccgcg cccccgcctc cacctttcac aaatgcccgg gtggtgaaat
     1801 aggtagacac aacggactta aaatccgtcg ggactaaaca tcccgtgccg gttcgattcc
     1861 ggctccgggc accaagctga aaatgacaat accgctccaa gcggttattt ttttatctgt
     1921 cggacggtga tggacaatat tgacatgttc atgcctgaac aagaggaaat ccaatcaatg
     1981 tggaaagaaa ttttactgaa ttacggtatt ttcctgctcg aactgcttac cgtgttcggc
     2041 gcaattgcgc tgattgtgtt ggctatcgta cagagtaaga aacagtcgga aagcggcagt
     2101 gtcgtactga cggatttttc ggaaaattat aaaaaacagc ggcaatcgtt tgaagcattc
     2161 tttttaagcg gggaagaggc aaaacatcag gaaaaagagg aaaagaaaaa ggaaaaggcg
     2221 gaagccaaag cagagaaaaa gcgtttgaag gagggtgggg agaaatctgc cgaaacgcaa
     2281 aaatcccgcc tttttgtgtt ggattttgac ggcgatttgt atgcacacgc cgtagaatcc
     2341 ttgcgtcatg agattacggc ggtgcttttg attgccaagc ctgaagatga ggttctgctt
     2401 agattggaaa gtccgggcgg cgtggttcac ggttacggtt tggcggcttc acagcttagg
     2461 cgtttgcgcg aacgcaatat tccgctgacc gtcgccgtcg ataaggtggc ggcgagcggt
     2521 ggttatatga tggcgtgtgt ggcggataaa attgtttccg ctccgtttgc gattgtcggt
     2581 tcggtgggtg tggtggcgga agtaccgaat atccaccgcc tgttgaaaaa acatgatatt
     2641 gatgtggatg tgatgacggc gggcgaattt aagcgcacgg ttacttttat gggtgaaaat
     2701 acggaaaagg gcaaacagaa attccgacag gaactggagg aaacgcatca gttgttcaag
     2761 cagtttgtca gcgagaaccg ccctcaattg gatattgagg aagtggcaac gggcgagcat
     2821 tggttcggtc ggcaggcgtt ggcgttgaac ttgattgacg agatttcgac cagtgatgat
     2881 ttgttgttga aagcgtttga aaacaaacag gttatcgaag tgaaatatca ggagaagcaa
     2941 agcctgatcc agcgcattgg tttgcaggcg gaagcttctg ttgaaaagtt gtttgccaaa
     3001 cttgtcaacc ggcgggcgga tgtgatgtag tttgccgaac ggatggaaat gccgtctgaa
     3061 gcgtgttcag acggcatttt tcaagttccg gctttgatgt tccgttccga ccactctatc
     3121 cgttgtccga tgtctttcaa attggaatat tgttcgacca atcgcggcac gtcgtccaaa
     3181 ctcatggcaa acatccacag ataagtgccg accgaataga catgccccgc gctgctgtag
     3241 cctttgagcg tcatcatcac aaaagcaaag ccgaacaaaa tacccatcgc cgcgccgacg
     3301 cagagatagc cgaaggcttc gcggttggaa atcagcacac gcaggcgcgc aaccagtccg
     3361 taatggcggt acagctgccg ccggtctcct tttcggataa agtggttgtc gcattccaag
     3421 ctgttgttca ggcggaaata caggttttcg ctaatggcgg caaaacgtgg caaaagccat
     3481 aaaaacaacg caagtatgcc caccgccgac acgccgaccc aaaattccag caccagcagc
     3541 atgatgcacg cgccgaatat ggatacgacg gatgtcgcgg caatcggcag gtgttcttca
     3601 aaaaagctga caaactcacg cgacagggca acccgcgcag ttaccgccga atgcgggact
     3661 tgtcgctgcc gctgttccaa cacgaccggc acggcgattt cggtataaat ccgcgtaaac
     3721 gtgcgcgtat cggcaatccg ccgcgccgca ccgaccagcc acattaaaag cacaaccaaa
     3781 gcgtacagca acgcctgcca caccttcccc gcaattacgg cattaattgc ccagccgcca
     3841 aacaccggat acaccagcat caaaaggttt tccagtccga ccagaaaaaa tgtgccaatc
     3901 aatcgcttgc ggtgggtttt ggctatgtgt ttcaacattt tccacatctt tgctccctat
     3961 tttttatttt ccgagattgt tttttccatc acttccgcca gtgcatccaa atcggcaaac
     4021 agccgagttg tgcgcttgtc gccgaatgtg gcaaatactt tgtcgctgaa ttcctgcgcg
     4081 ctttctgtta aaggtgcggc atgggctttg cctgtttcgg tcaacgacag caaccgttcg
     4141 cgccggtcct gttcgctttc ctgccattca atcaaccctt gtccggcaag ggttttgcat
     4201 acgcctgaaa cggtctgttt gggcagactc cactcttcgc cgatatgctt ttgcgtgcgg
     4261 ctgccttcgg ttgccagggt ataaagtacg gcaaagaggt tgtaattcaa atcctgctgc
     4321 ccgatccatt tgtcgaagac attgcaaatc aggttgatac gggtgccaag ttggtcgagt
     4381 tggttcataa ttggtcttga tattgactaa atttgtgcag gttatagtca taatcgggac
     4441 taattttaag cgtggggcga taaaaatgcc gtctgaaaat atttcagacg gcattgtgtt
     4501 tggaaaaggc aatcagtgtt ctgccaattt caggtaaacg ccggaaacgt cctgttcgcc
     4561 gtagccggct tcgactgctt tgcggtagct ggcagcaacg gtttcgacgg cgggcagggt
     4621 gttgcctgcc tgttcaagct ctttgacggc gaggttgagg tctttggagg cgtgtttgag
     4681 ggcgaaggcg ggtgggaatt cgcggtttgc ccacagggat tttttggttt ggaacatggg
     4741 cgagtccatt gccgagccgc cgatggcttc gacgatggtg tcggtatcga tgccgaactg
     4801 ccgcgccatc agcatcgctt cgctgtacgc ttcgccgaaa atgcccaaga gcgagttcaa
     4861 gacgagtttc gcgcccgaac ctttgccgac atcgccgaaa tggaaggttt ttttgccgac
     4921 gagggaaaat attttttgca gcgggtttaa aacggcttcg ctgccgccga acagaatcag
     4981 cagcgtgccg ttggtggcgg gcccgaccga tccggaaacg ggtgcttcgg caaactgtcc
     5041 gcctgcggct tcgacaagtg ctttgacggc gaggttttcg gtcggggaga tggtgctcat
     5101 gttgacgatg attttgccgg ccaatccgtc gcggactccg ttcaggatgt cgcacacggc
     5161 ggcatagtcg gaaaccatca ggaaaatgac gggatagtcg cggacgagtt cggcggtgtt
     5221 gccgtaaact tttgcgcctt tggcggagat gggggcagtt ttgtcgggcg agcggttgta
     5281 tacgccgact tcgatgccgc cgtccaagag ccgcgttacc ataggcagac ccatttgccc
     5341 taagcctatc cagccgattt gtgtgtattc gtttgcggac atgatgttta ctccttttct
     5401 gtttgtagtg tagtgtaatc ttatctgttc cgataccgtc tgaaaagcct gcgggtgttt
     5461 cagacggtat ggttttttat gcggtcagcg tgcctttggt cgagggggtc tgtcctgcca
     5521 tgcgcgggtc gtgttcgact gccatacgca gggcgcgccc gaaggctttg aatacggttt
     5581 ccgcctgatg gtgggcgttt ttgccgctga ggttgtcgat gtgcagggtc atcatactgt
     5641 ggttgacgat gccgtggaaa aattcttcaa acaaatcgac atcgaaacgt ccgattagtg
     5701 cgcgggtaaa ttcgatgttg tacacgagtc cggggcggcc ggaaaggtcg atgacgacgc
     5761 ggctgagggc ttcgtcgagc gggacgtagg aatgtccgta acggcggatg ccttttttgt
     5821 cgccgagtgc ctgccggatt gcttgtccga gtgtgatgcc gatgtcttcg gcggtgtggt
     5881 ggtcgtcgat gtgcaggtcg cctttgcagc tgatgtcgat gtcaatcatg ccgtggcggg
     5941 cgatttgatc gatcatgtgt tcgaggaagg gtacgccggt atccagcctg cttttgccgc
     6001 tgccgtcgag gttgatggag acggtgattt gggtttcgca ggtattgcgg ttgacggtaa
     6061 cgctgcggca gccggcagca gatgcggttt cggcaatttg ggtttcggca gttccggtgc
     6121 tttctgcggc ggtttcgggg acagtgcgtt cgcggtgttt tctgtcgagc cagcctttgg
     6181 gtttgccggt gtgtttttcg agttttgcca tcaggctggg acggatgccg cgtgcatttg
     6241 ggtcttctgc ctgcttttca aggcgttgtt tgagtttgta gagtgcgacg ggggtacggt
     6301 agccgcagag tttggcgagc ttggacagcg aacctgcttc ttgggcgagg gtcagaaagt
     6361 tgtgcagttg gcgttgtgtt ttagtcaaat tcataggaaa tccgttggtt ggcagtaaag
     6421 ttggcgaatg atgttgagaa cggcatcgtt ttgtgcgggg ctgcctacgg taatgcgcag
     6481 gcagtgttcc aaaagcgggt gcgcgccatg cagtttttta accaagatgc ggttttgttt
     6541 gagcgtgtca aacaacaaat cggcatcggg tacgcgtatg gtaatgaagt ttgcctgact
     6601 tggaaaggtg ttcagacggc atattttgcc caattcggcg aacatccgtt cgcgttcgtt
     6661 tttcaggctg tcgatgttgg cagagatgat gccgtagtgt tgcagggcga gtttggcggt
     6721 ggtcaggctc agttggttca tattgtaggg cggcaggatt ttttgcagtt cgccgatgac
     6781 ttcgggacag cctgccgcat agccgatacg cagtccggca aaaccgattt tgctgacggt
     6841 acgcatgacg atgaggttgg gaatgctgcc cgcctgcggc aggaagctgt cgccgttgaa
     6901 tgcgccgtag gcttcgtcga cgacgacgat gccgtctgaa gcttcgatga cggcttcgat
     6961 ttcggcacgc gtgaaacata cgccggtggg gttgttgggg taggcgataa aggtcagggc
     7021 agggcggtgc ttcctgacgg cttcgaggac ggcgggcagg ttgagggtga aatctccgtt
     7081 cagtggaacg ccgacataat ccatgccgta cagcgcggcg ttgtggcggt acatgacgaa
     7141 actgggttcg gctgccaaca ttgccgcgcc cggtttggcg gtcagcatcg tgatgaactg
     7201 tatcagctcg tccgagccgt tgcccagcgc gatgtcggcg cagtcgggaa tgtcgaacgc
     7261 cgaacgtaat gcttcctgta aaccgctgcc ggagggattg gggtaaagat ggatgggcgc
     7321 ggcggcaagc cgtgcctgcc attcctgcaa cagcgtttca tgtccggcaa aagggtggac
     7381 gggactttcc atcgaatcga gtttggcaaa gccgggcgga acgtcggcaa tctgatatgc
     7441 cgacatagct tgtatgtcgt cgcggatgaa ggagcggacg gatttcatcg tgtttcctta
     7501 atggttggaa tatgcctgta cgccgtttcg gcattatttc atacggaact ctgccgcgcg
     7561 ggcgtgggcg gtcaggcttt cgccgtgtgc cagcacgctg gcggtttcgc ctaatttttg
     7621 cgcgccctgt tccgaaacct gaatcaggct ggagcgtttt tggaaatcat atgtccccaa
     7681 aggcgaggaa aagcgggcgg ttcggctggt gggcaataca tggtttggac cggcgcagta
     7741 gtcgccgagg ctttcgccgg tgtagcgtcc catgaaaatc gcaccggcgt ggcggatttt
     7801 tttcgcccat tcctgcggat tttcgactga cagttccaag tgttcggggg aaatgtagtt
     7861 ggcgatttcg caggcttcgt ccaagtcttt ggcgagtatc atcgcgcccc tgttgccgag
     7921 cgaggcttcg atgatgtcgc ggcgcggcat agtttcgatc aggcggtcca tagcggcttc
     7981 tacttcgtcg agatacgctt gcgacgtgcc gatgaggatg gcttgggcaa tttcgtcgtg
     8041 ttcggcctgg ctgaacaaat ccatcgccac ccaatcggca ggtgtcgtgc cgtcggcgat
     8101 gaccaggatt tcagacggcc ccgccaccat gtcgatgccg accacgccga acacgcggcg
     8161 tttggcggcg gcgacgaagg cgttgcccgg accggtgatt ttatcgactt gggggatggt
     8221 ttccgtgccg taggcgaggg cggcaaccgc ctgcgcgccg ccgacggtga agactttggt
     8281 tacgccggcg acgtatgcgg cggcaagtac gatgtcgttg cgttcgcctt ttggtgtcgg
     8341 cacgaccatg atgatttctt tcacacctgc gacgtgggcg ggcatggcgt tcatgatgac
     8401 ggaactcgga tacgccgcct tgccgccggg gacgtaaatg ccgacgcggt caagcggtgt
     8461 gatttgttgt cccaacagcg tgccgtcttc atcggtgtag ctccacgatt ccattttttg
     8521 gcgttggtgg tagctttcga cacggcgggc ggcggtctgc aatgccgtct gaacgtcgtt
     8581 cggaatgcgc tcgaacgccg ctttcaaatc ggcttgcgtg agtattaaat catcgatgct
     8641 tttagcgttt gtctgatcga atttgttggt gtattcaatc aaagccgcat cgccgcgctt
     8701 ttgcacgtcg gcgcaaatgt cggcgacgat gcgttcggtt tcggggtttt gcgcggtttc
     8761 aaaagccagc agggctttga gtccggcttg gaaatcgggc gattgggtgt tgagtttttt
     8821 catgatttgg ttttttcctt ttttattttt ggattggggg tcgtctgaat attctcttgt
     8881 tcagtttctg tctctgtttt gggtctgata tacgcttttt ctcccccgct accaaacttc
     8941 aattcgggca ggcatttttg ttgcagaccg agtttggcta atacctcttc gctacgagag
     9001 gtagtgcgga aacggtaacg ggattgtttt ctaccgtttt tggtttcctc aaccattgtt
     9061 tcaaaaccgt ccacttcgta tgcactgaca acagcgtttt gcagaccggt gtgaacgccg
     9121 ataacgtatt tcacttttga agcaacatct ttaccgataa cccagttgcc caacgtacgc
     9181 gatttgaggt tggcatcgtc ttggttgtcg aaaaggtagt ctaattcttc gtcagtatct
     9241 aaatcgaaag cattgtggat tttgatggcg agaatcagcc cgtcggggtt aatctcgtta
     9301 agtgggagag aagagaatcc aaagcggcga tctagttctt ctacgctaat accacccgga
     9361 ccatgcccgg caattttatt tttgagtttt ttacccaaga cagatttaac aaaatgaatt
     9421 aaggcagatt cggcggcgag tgcttcgact tcagtcagat gatagctgat gatatagcga
     9481 ccgagtttct tgcatttgga gatggctttg agtttccgat cgataatctc gccggagact
     9541 gggtcttgtg aacgactagc aacccattca tgctcgaaga tacgattacc gaagcctttg
     9601 ccgatgtaga ggactttgtc ttttttcagg tcggtcagac aatagacata aaaccgacgc
     9661 tcgccgttat tcaaaacgga aagggttgaa tctgagaatt tttttatttt tgcaaccatt
     9721 ttaacttctc ctatacagtt tactgtcggt gatattttca gacgacctgt cggtgggaac
     9781 ggatagggtc gtctgaaaac gcatctttat tcaaatggat gcttacttcg ccttcactgc
     9841 gccgccgaac gcctgaataa tcggctccag cagcgcgtat ttcgttttca aagcagcctt
     9901 gttgaccacc aggcggctgg aaatgtcgac gatgtgttcg actgcttcca agccgtttgc
     9961 cttcaaggtg ttgcccgtcg aaaccaagtc cacaatcgca tcgctcaagc cgaccagcgg
    10021 cgcaagttcc atcgagccgt acagtttgat aatgtccaca tggacaccct tgccggcaaa
    10081 atgagatgcc gcgatttcag gatacttcgt ggcaatcttc agacggcatc cgggttgcga
    10141 agctgcttcg taatcaaacc ctttacgcac agcaaccatc atgcggcact tggcaatctc
    10201 caaatccaaa ggccggtaaa gccccgtgcc gccgtgttcg atcagcacgt ctttgcccgc
    10261 aatgccgaag tccgccgcgc cgtagcggac ataagtcggc acatcggttg cgcggacaat
    10321 gacaaggcgg atgttttcat ggttcgtccc gataatcagc ttgcgcgatt tttcaggctc
    10381 ttcagtcgga acaatgccgg cagcggcaag cagcggcagc gtctcctcaa aaatgcgccc
    10441 cttggataag gcgatggtca aagcattatc ctgcatggtg tggatgtcct ttgtcggttg
    10501 aaacaataat gccgtctgaa caggcgccga cggtgcagac ggcatacggt tttatcagag
    10561 cagggtcctt acatcggcag caatcgtttc cggctcgctt ccgtaaggcg agaaaatggc
    10621 aacctcaccg tttttgtcga tgagatacgc accggaagag tggtcgacca aatagttttc
    10681 gctgtcgtct ttttgattga ctttggcaga aaccacgcgg tattgctgct tgatgaccgg
    10741 caggttttgg ccgcccgttg ccgtcagacc gataaagtcc ggattgaact gtttggcata
    10801 cttgccgatg atttcaggcg tgtcgcgttc cggatcgatg ctgacgaaca ccactttcac
    10861 gtccttagcc tgcccgccca actgcttcaa cgtgtcgctg tacgtcaaaa gctctgtcgg
    10921 gcagacatcg ggacagtgcg taaagccgaa agacagaatc acgaccttgc ctttcaaatc
    10981 gctcaggctg aaaggcttgc cttcgccgtc ggtcagcgtg aaatcgccgc cgatgtcttc
    11041 cttacgcata tccgtaccgc gcgtttgcgg ctttgccgca ttttccgcag ccgacgcgga
    11101 tgcacttgaa gaagcggctt gcgccgcact gttgtcttga ggtttgcagg cggcaagcgc
    11161 ggcaagtacg aaaacgcccg gcaaaaagga acgcggtacg gaaaacatga caatctccca
    11221 agaataaata gcaaaacctg attcgtacgg aatttgaata tttccatctt aacgcgtttg
    11281 gcagatttaa tgtagtattt ttttacgttg tctgcacggc aggaattgcc gaatcataca
    11341 gaatctttgg agcggaaaga acagtaagat taaaataata aaaattctat ttaaaatcaa
    11401 aaataaaata aaaacagata aaataatttc aaaaaaaggt ttacagaatt tcaaaagcca
    11461 ttataatgac gacttcttca ccagcccagg tggcgaaatt ggtagacgca ggggactcaa
    11521 aatcccccgc cgcaaggtgt gtcggttcga gtccgaccct gggcaccaca accgctttta
    11581 gagcggttat ttttttgtct attgcagtct aacgccgtaa accaaagcca agaaagacaa
    11641 ggttttccgc tggtttcagt gcatatatat catctaatcc cgtctaatct gttctaattg
    11701 agttaaccta aaatttacgg tattttttac ggtatctgaa aaatacctcg aaaaaatac
//
LOCUS       DAXHRI010000048        11745 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXHRI010000048 DAXHRI010000000
VERSION     DAXHRI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.048"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(354..776)
                     /locus_tag="ACFM75_000985"
     CDS             complement(354..776)
                     /locus_tag="ACFM75_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC6255550.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(894..1565)
                     /locus_tag="ACFM75_000986"
     CDS             complement(894..1565)
                     /locus_tag="ACFM75_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC6255551.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1581..2207)
                     /gene="purN"
                     /locus_tag="ACFM75_000987"
     CDS             complement(1581..2207)
                     /gene="purN"
                     /locus_tag="ACFM75_000987"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC6255552.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAILNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2314..3132)
                     /locus_tag="ACFM75_000988"
     CDS             complement(2314..3132)
                     /locus_tag="ACFM75_000988"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6255553.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3391..3831)
                     /locus_tag="ACFM75_000989"
     CDS             complement(3391..3831)
                     /locus_tag="ACFM75_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC6255554.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3894..5300)
                     /locus_tag="ACFM75_000990"
     CDS             complement(3894..5300)
                     /locus_tag="ACFM75_000990"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC6255555.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNTDVHAFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5470..6585
                     /gene="lptF"
                     /locus_tag="ACFM75_000991"
     CDS             5470..6585
                     /gene="lptF"
                     /locus_tag="ACFM75_000991"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC6255556.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6582..7652
                     /gene="lptG"
                     /locus_tag="ACFM75_000992"
     CDS             6582..7652
                     /gene="lptG"
                     /locus_tag="ACFM75_000992"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC6255557.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7952..10537
                     /gene="acnB"
                     /locus_tag="ACFM75_000993"
     CDS             7952..10537
                     /gene="acnB"
                     /locus_tag="ACFM75_000993"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC6255558.1"
                     /translation="MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10679..11674
                     /locus_tag="ACFM75_000994"
     CDS             10679..11674
                     /locus_tag="ACFM75_000994"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC6255559.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLNLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDG
                     IEYRGFAQETVEELTKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2699 a         3259 c         3052 g         2735 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta taattcaata aattaatata
       61 tggcttaaaa taacgggatg cccgcctccc gtcctcctgc agaagcgggc gggtatcatt
      121 ttaaaacgcg gcatttaaaa tttgaccgaa aattgttgac aatccggaat caagtctgca
      181 caataccccg acaagtccaa gtattataaa ggctgaataa agaggaaaca gcaggcagat
      241 atattcggga ggtgcagtcc gaatatatct gcttttttat gcgcctccgg attgcctgcc
      301 gcacctttcc cttcagacgg catcagccgt ttccccataa tgccgcccga tgcctattta
      361 tctgccccgg caatttcaaa actgtgggta atctttgccg ctttgcccaa cataatcgaa
      421 gccgaacagt atttttcggc agacatctga acggcgcgct caatggccga ttctttcaaa
      481 tcatgcccga atactttgaa atggatgtgg atttcggtaa acacgcgcgg cgcatcgtcc
      541 gcccgtttcg ccgtaaccgt cgcacggcag tcagtcactt tctgacgctg tttttcggca
      601 atcatcacca catcgatgct cgaacagccc gccacgccca acagcagcat ttccaaaggg
      661 ctgggcccgc gcttagcctt accttctgcc gccgacccct ccataacgac gctgtgcccg
      721 ccttccgttg ttccgacaaa gcacattccg tctatccatt ttgatgtaac ctgcatggtg
      781 tcattcctga aaatagcgtt aaaaccgctt tgcatatggc gttattgtaa acaatttcaa
      841 gcggcttatg cagaaatatg gacaaaacgg caaaaaaacg cctgaaaatc gatttacggt
      901 ttggcggact ggccattgat ctgcaccgat ttgagtttca gcgtataggt tttgccgtcg
      961 tcggtatagc cgatttgtgc cggaatattg ttcagggacg gtgcgaagaa atacattacc
     1021 gtatcgtcgc cgcgccgcac ccgatatttg acgacttcgg tttccacgcc gcctatgctg
     1081 tattttcctg tacccgcctt attcaaaccg ccgacggaat aaagtttttt gccgttggtg
     1141 attttcagcc ccggggggag tttcgcgtca tttgccgcca actgccaggc aagcgtgaac
     1201 aaatccatag ccttggggct ttgctcggtt ttgctctcgc ccgctttgcc gtaggttacg
     1261 ctgccgtcgg cgaatttggc ttccgcatac agtttgcccc tgcgtatgtc tctatagtag
     1321 gtagggtgca gggtattgcc gacaaccgta ccgccggact cgaaacggat attgtatagc
     1381 ggcactttaa tcgtcgaaac gattttgtaa gcattgccgc tgcgttcaaa tgtcatcgtg
     1441 gcgggaatgc cgtagctgcc ggaatagtgc agcacggcgg attggggcag ccctgccgca
     1501 tacgcgcacg gcagggcggc ggacaaaatg gcggcggaaa atatattttt aaaagtcttc
     1561 atcatttgct cccgcccggt ttacgccgtc aggaaacggg cggcatcggc gttttccgaa
     1621 tttctgacgc ggtttccctc gataatcagg cggccggcgg caaaatcggc aacggctttc
     1681 ggatagagtt tgtgttcgac agccaaaacc cgggcggcaa catcgtctgc cgtatcgccg
     1741 tcgagtatcg gcacaatccc ttgcgataca atcgggccgc aatccagttc ggcagtaacg
     1801 aaatggatgg tgcagccggc aacgcggcag cccgcctcca aagcgcgttc gtgcgtatga
     1861 agtccggtaa acgagggcag gacggacggg tgaatgttca tcagcctgcc ttcgtaatgg
     1921 gcgcaaaact caggggtcag aatccgcata aaacctgcca aaaccaccaa gtcgggttga
     1981 tatgcgtcga ttttttccat catggcggta tcgaaggcaa gtcgtgattc aaagttttta
     2041 tgattcaggc tgtcggtcgg aataccctga ccggccgccc attgcaaacc ggcagccgtt
     2101 tcgctgttgc tcaacacggc ggcaatacgg acgttgagaa tggcggcatt gacgattgcc
     2161 tgcatattgc tgccgcgtcc ggaaatcagg atgacgatgt ttttcataat ggtgcgcttt
     2221 tgaaagggat gccatctgaa ccgctgtttg gtggtttcag acggcatttg ccgtaaaaat
     2281 gcccgaaaac ctgtttcggg catggattcg gacttaattt acttttttga tgtcgacttg
     2341 agccggctgc ttggcgggcg cgttttcggg tgcgccgatt ttgaccagtt tcacatcaaa
     2401 taccaaagtg gcgttcggac cgattttgtc gcccgcaccc tgttcgcggt aggcaaggtt
     2461 ggacgggatg tagaacgtgg cttcgccgcc ttctttcaga agctgtacgc cttcggtcca
     2521 acccggaatc acttggctca aagggaaggt gaccgggccg ccgttggctt tgctgctgtc
     2581 gaataccgta ccgtcaatca ggcggccttc gtattccacg gtaacgatgt cgtctttggt
     2641 cggctgtttg ccttcaccct gtttggtgat tttgtattgc aggccggaag cagtggtctt
     2701 cacgccgtct ttggcggcat tttctttcag aaaggcttcg cctttttctt tattggcctt
     2761 cgcgtccgcc ttgtgttttt ctacggcttt agcctgttgt tcctgaagga atttcatcat
     2821 gacttcctga gcctgctctt cggtcatttt gatttctttg ccgtcataca ctgcctgcat
     2881 ggcttcggta aagactttca aatcgatttc cgcgccctgt tccttcattt gcttcaggga
     2941 gcgtccgatg tccacgccca tcgcatagct tgcctgctgc atcgtgctgc cgatcgaaga
     3001 ggtgtcgccc tgcgcggaag aagcggcggc aggttcggat gcagatgcgg gggcggcttc
     3061 ttttttgccg caggcggaaa gtgccaaagc ggcggaaagg gtcagtgcgc tgattttgaa
     3121 aatggtgttc atgatggatc ttcgctgtcg ataaggtcgg aaaaacggga ttatagccga
     3181 gtttgaatgt ttcaacacac aggatgacac ataaagcgtc aatcgtgtgt tgccctgttt
     3241 tggaaaggat tgaaccttcc aaaataagtt ttgattctac cgccccgagg gacagatgtc
     3301 caagtggcgg ggttcaaccg ataaggaaat tttaatcaaa tagaatcaag cctgtttaaa
     3361 ttttgtaaat gcggcatttc agacggcatt ttatgccttg ccctccatgc cgtgatgttc
     3421 gatggcaaaa ccgcttcggc ggtaggcggt aaagcgttcg cgcgcgtcgg cgagatcttc
     3481 caagctgttg ccgacgattt ccaaaacgcg cgcgggcaag accggagcgg tgttccaaaa
     3541 accgtcggac aggttcaaaa cagtcatgcc ttcgggaatt cggggcggat tgccgccgca
     3601 agcaagcagg acggatgttt cagacggcat ggcttcttcc atttcccaaa tttcgtgcgg
     3661 aataaaactc tcggcctcgt attgccaaag cattttgtcc aattcctgaa gctgcccgaa
     3721 cgaatcggac cacaccagta tcctgccgcc gtcccgaatc gcacgggcaa tcaggcggca
     3781 ggtgaaaatc ggaacttggg caacgtgcgt gtaaaaggtg gctttcggca tattgtttga
     3841 gcatttggca ggacgatgcc gtctgaaagg cttcagacgg cattgtggca aaattaaaga
     3901 ttccgcagat agttcagcag caagggaacg ggacggccgg tcgcaccttt ttccgcaccg
     3961 gatttccacg ccgtacccgc gatgtcaagg tgtgcccatg gatagtcttc ggtaaagtag
     4021 gataggaatg ttgcggcggt aatcgtgccc gcgccgggcg tgccgatgtt tggaatgtcg
     4081 gcaaagttgg atttgagttg gtctttgtag gtctcaaaga gcggcagttg ccacgctttg
     4141 tcgtccacgt tgtaggaagc ggcaagcagg ctgtcgacca aatcttgatt gttgcccatc
     4201 acgccgctga catcgtgccc caaggcaaca atacacgcac cggtcagggt ggcgacgtcg
     4261 atgacggctt tgggtttgaa ctgttcggcg taagtgagcg cgtcgcacaa aatcagacgg
     4321 ccttcagcat cggtgttcaa cacttcgatg gtcaggcctt tcatgctttt tacgacgtcg
     4381 cccggtttgt tggccgcgcc ggaaggcata ttttcacaag tggcgacgat ggcaatcagg
     4441 ttgatcggca gttgcagttt gacggcggcg cagaaggtgc tgatgacggt tgccgcgccg
     4501 cacatatcaa acttcatttc gtccatgttc agaccgggtt tgagggaaat gccgccggtg
     4561 tcgaaggtaa tgcctttgcc gaccaagacg acgggggcgg cttctttgtc ggctgcaccg
     4621 aaatagctca gttcgaccaa atagggggct tccgcgctgc ctttggcgac cgaccaaaac
     4681 gaacccatgt tttctttgat gtagtctttt tcgatgattt tggcgtgcgc gcccagtttt
     4741 tcggcttcgg ctttggcggt gcgcgctaaa aattcgggcg tgcattcgtt gggcgcggcg
     4801 ttgcccaagt cgcggcagag gctttgtccg taaacttgcg cttcggcgac gcgcaaggct
     4861 tctttaacga cggcttcgtg tgcgctgtgg aacacggcgg tttcaaattt ggcgggcttg
     4921 gcttcttttt tgtagcggtc gaaacggtag gcggcgttgc cgaacgcaat cgcaaacgct
     4981 tcggctacgg cggcggcttg cgcttcttca aaggcgtgaa cgtccgtatt gaccgtttcc
     5041 tgattttgcg cccatttggc ggcttcggcg gcggccttgt tcaatgcggc gcggccggtg
     5101 cttttcagac agcatacggc aacagcctgc aaaccgttgc ctgtcgggat ttttgtgtcg
     5161 gcaaaatttt gaccttcttc aagcgaagac aaaagggcaa gggcggtcgg atggtttaat
     5221 tgcgcctctt cggtgcagac aaataactgt gcgcctgcct gctgttcctg caagatttcg
     5281 gtttttgtgc taaattccac gtttattctc ctgattgaga cggttgtcgg tagttttcgg
     5341 acggcctttc gctcaaaaga ccgtctgaag acggctggca cgattgtacc ccatttgaag
     5401 caccgtctga aaccttgcgc ggacaatccg cctgcgccga accgcttacc gccccctgac
     5461 cgcgattcta tgatttatca aagaaacctc atcaaagaac tctcttttac cgccgtcggc
     5521 attttcgtcg tcctcttggc ggtattggtc tccacgcagg caatcaacct gctcggccgt
     5581 gccgccgacg ggcgtgtcgc catcgatgcc gtgttggcat tggtcggctt ctgggtcatc
     5641 ggtatgacgc cgcttttgct ggtgttgacc gcatttatca gtacgttgac cgtgttgacc
     5701 cgctactggc gcgacagcga aatgtcggtc tggctatcct gcggattggc attgaaacaa
     5761 tggatacgcc cggtgatgca gtttgccgtg ccgtttgccg ttttggttgc cgtcatgcag
     5821 ctttgggtga taccgtgggc agagctacgc agccgcgaat acgctgaaat cctgaagcag
     5881 aagcaggaat tgtctttggt ggaggcaggc gagttcaaca gtttgggcaa gcgcaacggc
     5941 agggtttatt ttgtcgaaac cttcgatacc gaatccggca tcatgaaaaa cctgttcctg
     6001 cgcgaacagg acaaaaacgg cggcgacaac atcatcttcg ccaaagaagg taacttctcg
     6061 ctgaacgaca acaaacgcac gctcgaattg cgccacggct accgttacag cggcacgccc
     6121 ggacgcgccg actacaatca ggtttccttc caaaaactca acctgattat cagcaccacg
     6181 ccaaaactca tcgaccccgt ttcccaccgc cgtaccattc cgaccgccca actgattggc
     6241 agcagcaacc cgcaacatca ggcggaattg atgtggcgca tctcgctgac cgtcagcgtc
     6301 ctcctactct gcctgcttgc cgtgccgctt tcctatttca acccgcgcag cggacatacc
     6361 tacaatatct tgattgccat cggtttgttt ttaatttacc aaaacgggct gaccctgctt
     6421 tttgaagccg tggaagacgg caaaatccat ttttggctcg gactgctgcc tatgcacatc
     6481 atcatgttcg tcatcgcaat cgtacttctg cgcgtccgca gcatgcccag ccagcccttc
     6541 tggcaggcgg ttggcaaaag tctgacattg aaaggcggaa aatgaacctg atttcacgtt
     6601 acatcatccg tcaaatggcg gttatggcgg tttacgcgct ccttgccttc ctcgctttgt
     6661 acagcttttt tgaaatcctg tacgaaaccg gcaacctcgg caaaggcagt tacggcatat
     6721 gggaaatgct gggctacacc gccctcaaaa tgcccgcccg cgcctacgaa ctgatgcccc
     6781 tcgccgtcct tatcggcgga ctggtctctc tcagccagct tgccgccggc agcgaactga
     6841 ccgtcatcaa agccagcggc atgagcacca aaaagctgct gttgattctg tcgcagttcg
     6901 gttttatttt tgctattgcc accgtcgcgc tcggcgaatg ggttgcgccc acactgagcc
     6961 aaaaagccga aaacatcaaa gccgccgcca ttaacggcaa aatcagcacc ggcaataccg
     7021 gcatttggct caaagaaaaa aacagcatta tcaatgtgcg cgaaatgttg cccgaccata
     7081 ccctgctggg cattaaaatc tgggcccgca acgataaaaa cgaactggca gaggcagtgg
     7141 aagccgattc cgccgttttg aacagcgacg gcagttggca gttgaaaaac atccgccgca
     7201 gcacgcttgg cgaagacaaa gtcgaggtct ctattgcggc tgaagaaaac tggccgattt
     7261 ccgtcaaacg caacctgatg gacgtattgc tcgtcaaacc cgaccaaatg tccgtcggcg
     7321 aactgaccac ctacatccgc cacctccaaa acaacagcca aaacacccga atctacgcca
     7381 tcgcatggtg gcgcaaattg gtttaccccg ccgcagcctg ggtgatggcg ctcgtcgcct
     7441 ttgcctttac cccgcaaacc acccgccacg gcaatatggg cttaaaactc ttcggcggca
     7501 tctgtctcgg attgctgttc caccttgccg gacggctctt cgggtttacc agccaactct
     7561 acggcatccc gcccttcctc gccggcgcac tacctaccat agccttcgcc ttgctcgccg
     7621 tttggctgat acgcaaacag gaaaaacgtt gaaccaatgc cgtctgcccc ccttcggacg
     7681 gcattggttt ttattatcat attcccgtag acagatagcc gttccctatt atagtggatt
     7741 aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta ttgtactgtc tgcggcttcg
     7801 tcgccttgtc ctgatttaaa atacattacc tgtcataaca gttccatttt tgttaaaact
     7861 agtctatgat agcggtacaa atattgttta caatatttaa cgcaaatcat ttgcaacccg
     7921 acaaaagaaa aacagaaaaa ggaacaaaga gatgttagaa gcctatcgta aagccgccgc
     7981 cgagcgcgcc gcccttggta ttcccgccct tcctctgact gctcagcaaa ccgccgattt
     8041 ggttgagctg ctgaaaagcc cgcccgcagg cgaaggcgag ttcttggtcg aactgcttgc
     8101 ccaccgtgtt ccgcccggtg tggacgatgc cgccaaagtc aaagcctcat tcctggctgc
     8161 cgttgccgaa ggcagcgcgt ccagcccgct gatctccccc gaatatgcga ccgaactctt
     8221 aggtacgatg ctcggcggtt acaatatcca cgccttaatc gaactcttgg acgacgacaa
     8281 actcgcgccc attgccgcca aaggcttgaa acatacgctt ctgatgttcg attccttcca
     8341 cgacgttcag gaaaaagccg aaaaaggcaa caaatacgcg caagaagttt tgcaatcttg
     8401 ggcagatgcc gaatggttcg cctcacgcgc caaagttccc gaaaaaatca ccgttaccgt
     8461 tttcaaagtt gacggcgaaa ccaatacaga cgacctctcc cccgcgcccg acgcgtggag
     8521 tcgtcccgat attccgctgc acgcgctggc catgctgaaa aacccgcgcg acggcatcac
     8581 gcccgacaaa ccgggcgaag tcggtccgat taaattgttg gaagaactca aagccaaagg
     8641 ccatccggtt gcttacgtcg gcgacgtggt cggtactggt tcttcacgca aatccgcgac
     8701 caactccgtc atttggcata ccggcgaaga cattccgttc gtgccgaaca aacgcttcgg
     8761 cggcgtatgt ttgggcggca aaatcgcgcc gattttcttc aatacccaag aagattccgg
     8821 cgcgctgccg attgaagtcg atgtatctgc tctaaaaatg ggtgatgtcg tcgatatcct
     8881 gccttatgaa ggcaaaatcg tgaaaaacgg cgagactgtt gccgagtttg aattgaaatc
     8941 acaagtattg ctggacgaag tgcaagccgg cggccgtatc aacctgatta tcggccgagg
     9001 tctgaccgcc aaagcgcgcg aagccctgaa actgcctgcc tctactgcat tccgcctgcc
     9061 gcaagcgcct gccgaaagca aagccggttt caccttggcg caaaaaatgg tcggccgcgc
     9121 ctgcggtctg cccgaaggac aaggcgtgcg tccgggtact tactgcgaac cgcgtatgac
     9181 gacggtcggc tcgcaagaca cgaccggccc gatgacccgc gacgagttga aagacttggc
     9241 ttgtttgggc ttctccgccg atatggtgat gcagtctttc tgccacaccg ccgcctatcc
     9301 gaaacctgtc gatgtaaaaa cccataaaga actgcccgcc tttatttcca cccgtggcgg
     9361 cgtgtcactg cgtccgggcg acggcgtcat ccactcgtgg ctcaaccgcc tgctgctgcc
     9421 cgataccgtc ggcaccggcg gcgacagcca tacccgtttc cccatcggta tttccttccc
     9481 cgccggctcc ggcttggttg cctttgccgc cgcaacgggc gtaatgccgc tcgatatgcc
     9541 cgagtctgta ttggtacgct tcagcggcaa gctgcaaccg ggcgtaaccc tgcgcgattt
     9601 ggtgaacgcc atcccgctgt acgcaattaa acaaggtttg ctgaccgttg ccaaagccgg
     9661 taagaaaaac atcttctctg gccgcatcct cgaaatcgaa ggcctgcccg atttgaaagt
     9721 ggaacaagcc tttgaattga ccgacgcatc cgccgaacgc tccgccgccg gctgtaccgt
     9781 gaagctcaat aaagagccga ttatcgagta catgaaatcc aacgtcgtgt tgatgaaaaa
     9841 catgattgcc aacggctatc aagacccgcg cactttggaa cgccgcatca aagctatgga
     9901 aaaatggctg gcaaatcccg agttgctcga agcggataaa gatgccgaat acgccgccgt
     9961 gattgaaatc aacatggacg acatcaaaga gccgattatc gcctgcccga acgacccgga
    10021 cgacgtgtgc ttcatgtccg aacgctccgg caccaaaatc gacgaagtat tcatcggttc
    10081 gtgtatgacc aacatcggcc acttccgcgc cgcctccaaa cttttggaag gcaaggcaga
    10141 cacccccgtc cgcctgtgga ttgcgccgcc gaccaaaatg gacgcgaaac aattgtccga
    10201 cgaaggacac tacggcgtac tcggacgtgc cggcgcgcgt atggaaatgc cgggttgctc
    10261 cttatgtatg ggtaatcagg cgcaagtacg cgaaggtgcg accgttatgt ccacctccac
    10321 ccgcaacttc ccgaaccgtt tgggtaaaaa cacctttgtt tacctcggtt cggcggaatt
    10381 ggcagcgatt tgctccaaac tgggtaaaat cccgaccgtt gaagaatatc aagccaatat
    10441 cggcatcatc aacgaacagg gcgataaaat ctaccgctat atgaacttca acgaaatcga
    10501 cagctacaac gaagtagccg agaccgtgaa cgtttaatcc ccgtcatccg tatgaagtaa
    10561 gggattgacc gcaatgccgt ctgaacaacc ttcagacggc attgcaacat tccgctaacc
    10621 cttctttccg caaacgctgc aaatacggcg ttcacgcccc cacataaagg aaacgacagt
    10681 gaacctgaaa aaccgccatt ttctgaaact tttagacttc acgccagaag aaatcaccgc
    10741 ctacctcaac cttgccgccg aattgaaagc cgccaaaaaa gcagggcgcg agattcagcg
    10801 gatgaaaggg aaaaacatcg ccctgatttt tgaaaaaacc tctactcgga cgcgctgcgc
    10861 gtttgaagtc gccgcgcgcg atcaaggcgc gggagtgact tatttagagc cgtccgccag
    10921 ccaaatcggg cataaggaaa gcatcaaaga caccgcccgc gtgttgggca ggatgtacga
    10981 cggcatcgaa tatcgcggct tcgctcagga aactgtcgaa gaattgacga aatacgcggg
    11041 cgtacccgtg ttcaacgggc tgaccaacga gttccatccc acacaaatgc ttgccgacgc
    11101 actgactatg cgcgaacaca gcggcaaacc tttgaaccaa accgcgtttg cctacgtcgg
    11161 cgacgcgcgt tacaacatgg gcaattccct gctgatttta ggggcaaaat tggggatgga
    11221 cgtgcgtatc ggcgcaccgc aaagcctgtg gccgtctgaa ggcattattg ccgccgcaca
    11281 cgccgccgcc aaagaaaccg gcgcaaaaat taccctgacc gaaaacgcgc atgaagccgt
    11341 gaagaatgtt gattttattc ataccgatgt gtgggtcagc atgggcgagc cgaaagaagt
    11401 ctggcaggaa cgcatcgatt tgctgaaaga ttaccgcgtt acgcccgaac tgatggcggc
    11461 atcgggcaat ccgcaagtca aattcatgca ctgcctgccc gccttccaca accgcgaaac
    11521 caaagtcggc gaatggattt acgaaacctt cgggctgaac ggtgtggaag ttacagaaga
    11581 aatattcgaa agccccgcca gcatcgtgtt cgatcaggcg gaaaaccgta tgcacacgat
    11641 taaagcggta atggtcgcgg ctctgggcga ctgacagaac tgtgcctgtt taaattcatc
    11701 cgcaacacag ataccgtctg aacacgatgt tcagacggta tccat
//
LOCUS       DAXHRI010000049        11415 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXHRI010000049 DAXHRI010000000
VERSION     DAXHRI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.049"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..79)
                     /locus_tag="ACFM75_000995"
     CDS             complement(<1..79)
                     /locus_tag="ACFM75_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6255560.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="ACFM75_000996"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="ACFM75_000996"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC6255561.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="ACFM75_000997"
     CDS             complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="ACFM75_000997"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC6255562.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSENKPAAKPTYTQVF
                     GKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLACE
                     GMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSFLR
                     CVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGVPVSDGMETVEIGKGII
                     RREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVTLE
                     ENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVERRV
                     RAWLSDRDAAN"
     gene            complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="ACFM75_000998"
     CDS             complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="ACFM75_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC6255563.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQGLSQRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4798..5862
                     /gene="fba"
                     /locus_tag="ACFM75_000999"
     CDS             4798..5862
                     /gene="fba"
                     /locus_tag="ACFM75_000999"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC6255564.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            5882..6844
                     /locus_tag="ACFM75_001000"
     CDS             5882..6844
                     /locus_tag="ACFM75_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC6255565.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCFSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKGLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGKLITLESGE
                     FQVYKQSHSALTALQTEQVQDSEDSGKMVAKRQFRIGDIAGEHTSFDKLPKGGSATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGHGKIEHLKSPELNVELATAYIKPDEKRHAVISGS
                     VLYNQDEKGSYSLGIFGGQAQEVAGSAEVETANGIHHIGLAAKQ"
     gene            6984..7661
                     /gene="tsaB"
                     /locus_tag="ACFM75_001001"
     CDS             6984..7661
                     /gene="tsaB"
                     /locus_tag="ACFM75_001001"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC6255566.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETRLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            7658..8098
                     /gene="rimI"
                     /locus_tag="ACFM75_001002"
     CDS             7658..8098
                     /gene="rimI"
                     /locus_tag="ACFM75_001002"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC6255567.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFANLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8092..8835
                     /locus_tag="ACFM75_001003"
     CDS             8092..8835
                     /locus_tag="ACFM75_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6255568.1"
                     /translation="MLSARYLHLHEALGLGPMWLKRGAVVLPSATLPESPTQIRPQKQ
                     TVLSIPQRPSEQHTGQARLETMKALETAAVPTRKPAPETETPLSGVSDGIAPVPAASG
                     ITKLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNP
                     MPSEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPA
                     RLLRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8897..9538
                     /gene="pyrE"
                     /locus_tag="ACFM75_001004"
     CDS             8897..9538
                     /gene="pyrE"
                     /locus_tag="ACFM75_001004"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC6255569.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9612..10046
                     /locus_tag="ACFM75_001005"
     CDS             9612..10046
                     /locus_tag="ACFM75_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC6255570.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10043..11353
                     /gene="argA"
                     /locus_tag="ACFM75_001006"
     CDS             10043..11353
                     /gene="argA"
                     /locus_tag="ACFM75_001006"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC6255571.1"
                     /translation="MSAPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVFGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYGGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEAELPEARRKAYRNNGRNSHILVRRLHR"
BASE COUNT         2566 a         3583 c         2989 g         2277 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatac agccgtcctg
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtt aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg cattgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acattgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgca cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaacgat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccagct ccaagcccgc accgccaaca
     1501 accgcgtcgt caactttacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacactttc tccctgcgcg gcaaagttgt cgaagcctaa acccttacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgatgg
     1681 cgcggccagg cggcttaatt tgccgcatcc cgatccgaca accacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttctcacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcacg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat gttcggcacg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcgtgggtcg ggccgtccgc gccgacgatg cccgcacggt cgacggcaaa caaaacgggc
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caataccgac atcgaaatag cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaatacttgg gtataggtcg gtttggcggc aggtttgttt
     2641 tcagacggca tttgcgccgc gctttctttg ggcaggttgg cgacggcgtg gtatttgacg
     2701 ggatcgtttt cggcgagttt gtagccgttg ccttttttgg taatgacgtg cagcaactga
     2761 gggcctttgc ggctgcgcaa gtctttcaat acgtccacca gattttcgac gttgtgtccg
     2821 tccacggggc cggtgtagcg gaagccgaag ttttcaaaca aagacagcga ctgtttggcg
     2881 tgttcggctt cttcggcaag ggttttgatt ttgtgttcga ctttttgggc aaactccatc
     2941 gcgccgggta ttttgtctaa taccttgccc gtttgcgctt tgacggtact caacaggccg
     3001 tgcatatcgc gcacgacgtt gctggcaagg tatttcggca gcgcgccgac gttgggggaa
     3061 atcgacattt cgttgtcgtt gaggacgacc agcaaatcca catccatatc gcctgcgcaa
     3121 ttcaaggctt caaacgcctg ccccgccgtc atcgcgccgt cgccgatgat ggcgacgctg
     3181 cggcggtcgc tgcccaagag tttgtctgcc gccgccatgc ccaacgccgc gccgatggag
     3241 gtggaggaat gccccacgcc gaacgcgtcg tactcggact cgcaacgttt cggaaaaccc
     3301 gccaaaccgc catattggcg catggtgtgc atctggtttt tcctgcctgt caggattttg
     3361 tgcggatagc tttggtgtcc gacatcccac accagcttgt cttcgggcgt atcgtacaca
     3421 tagtgcaggg cgatggtcag ttcgaccgcg cccagattgc tggcgaaatg accgccggtc
     3481 tgcccgacag attccagcag aaaggcgcgc agctcgccgg caaggcgcgg cagctgtttt
     3541 ttgtccagac ggcgcaaatc ttgcgggctg tcaatcaggt cgagtagggg gcttgggttc
     3601 atggtgtgtc ttttttatgt gtcgtccagg tgcaacgacc aattatatat caagagcgtg
     3661 cggctgacgg ctgattttgc cgtatgtcat tcgtcctgcc gcttggcgcg cgggtgggct
     3721 tcgtcataca ggcgggcgat gtggtcgaaa tcgagtttgg tgtaaatctg cgtggtcgaa
     3781 aggctgctgt gcccgagcag ctcctgcacc gcccggatgt cgcgcgaagc ctgcaacagg
     3841 tgtccggcat agctgtggcg catcatatgc ggcgaaacgt gcctgccgtc gccgttttgc
     3901 gccgcccatt gcgccaaacg tttttggatt tggcgttggc tcaggcgcgt gccgttcctg
     3961 ccggtaaaca gggctttgcc gtccgatgcc gtctgacgca gcggcagata gtttttcagg
     4021 gcttccacgc ttttgccggt cagcggcacc tgccgctgct tgcgcccttt gccggtaacg
     4081 tgtacccacg cctcgtccaa atagacatca tctgcattca agccgtgtat ctcgctcacg
     4141 cgcaaaccgc tgccgtacat cagttcgaac agcgcgtgat cgcgcaccgc cagcgggtcg
     4201 ccgccgtcca cgggcaaatc cagcatccgg ttcagccatt cctgcggcag tgctttgggt
     4261 acgcgctcgg gctgcttcgg cggtttgatg tcggcggtcg ggtcggcgcg catcagcccg
     4321 cgtttgacca gccaggcgca atactgccgc caagacgaca gcttgcgcgc cagcgtccgc
     4381 gcattcaaac cgcgctgcga cagccgccgc aacgccgccg taaaatcgcg ttgcgacaag
     4441 ccctgcggca cgccgccttc agcttcagac ggcatttgtg ccaacagcgc aaacagttct
     4501 tccaaatcgc gccggtatgc cgccaccgtg tgctccgact tgccctcgcg cacgatgttt
     4561 tccaaataag cgtcaaaatg cgccgcaaac ccgtccaaaa ccataaccgc cccacacaaa
     4621 tatcaaaaaa acagtgaaaa cattatcata aacgacacac caaaaccccg aaccgcccaa
     4681 aatccgaccc gtccgcccgc cgttatcgtt aacataaaat caatgagccg tttatggttt
     4741 tttgccgcaa aaaacattat aatccgcctt atttacctat tgcccaagga gacacaaatg
     4801 gcactcgtat ccatgcgcca actgcttgat catgctgccg aaaacagcta cggcctgccc
     4861 gcgttcaacg tcaacaacct cgaacaaatg cgcgccatta tggaagccgc cgaccaagtc
     4921 aacgcgcccg tcatcgtaca ggcgagtgca ggtgcgcgca aatatgcggg tgcgccgttt
     4981 ttacgccacc tgattctggc ggcagtcgaa gaatttccgc acatccccgt cgtgatgcac
     5041 caagaccacg gcgcatcgcc cgacgtgtgc caacgctcca tccaactggg cttttcctcc
     5101 gtgatgatgg acggttcgct gatggaagac ggcaaaaccc cttcttctta tgaatacaac
     5161 gtcaacgcca cccgtaccgt ggttaatttc tcccacgctt gcggcgtatc cgtcgaaggc
     5221 gaaatcggcg tattgggcaa cctcgaaacc ggcgaagcag gcgaagaaga cggtgtaggc
     5281 gcagtgggta aactttcgca cgaccaaatg ctgaccagcg ttgaagatgc cgtgcgtttc
     5341 gttaaagata ccggtgttga cgcattggcg attgccgtcg gcaccagcca cggcgcatac
     5401 aaattcaccc gtccgcccac aggcgacgta ttgcgtatcg accgcatcaa agaaatccac
     5461 caagccctgc ccaatacaca catcgtgatg cacggttcca gctccgttcc gcaagaatgg
     5521 ctgaaagtca tcaacgaata cggcggcaat atcggtgaaa cctacggcgt gccggttgaa
     5581 gaaatcgtcg aaggcatcaa acacggcgtg cgcaaagtca acatcgatac cgacttgcgc
     5641 cttgcttcca ccggcgcggt acgccgctac cttgccgaaa acccgtccga cttcgatccg
     5701 cgcaaatatt tgagcaaaac cattgaagcg atgaagcaaa tctgcctcga ccgctacctc
     5761 gcgttcggtt gcgaaggtca ggcaggcaaa atcaaaccgg tttccttgga aaaaatggca
     5821 aaccgttatg ccaagggcga attgaaccaa atcgtcaaat aacaggttgc ttgtaaacaa
     5881 aatgccgtct gaaccgttgt tcggacggca tttgattttt gcttctttga cctgcctcat
     5941 tgatgcagta tgcaaaaaaa gataccataa ccaaaatgtt tatatattat ctattctgcg
     6001 tatgactagg agtaaacctg tgaaccgaac tgccttctgc tgcttttctc tgaccgccgc
     6061 cctgattctg accgcctgca gcagcggagg gggcggtgtc gccgccgaca tcggtgcggg
     6121 gcttgccgat gcactaaccg caccgctcga ccataaagac aaaggtttgc agtctttaac
     6181 gctggatcag tccgtcagga aaaacgagaa actgaagctg gcggcacaag gtgcggaaaa
     6241 aacttatgga aacggcgaca gccttaatac gggcaaattg aagaacgaca aggtcagccg
     6301 cttcgacttt atccgtcaaa tcgaagtgga cgggaagctc attaccttgg agagcggaga
     6361 gttccaagtg tacaaacaaa gccattccgc cttaaccgcc cttcagaccg agcaagtaca
     6421 agactcggag gattccggga agatggttgc gaaacgccag ttcagaatcg gcgacatagc
     6481 gggcgaacat acatcttttg acaagcttcc caaaggcggc agtgcgacat atcgcgggac
     6541 ggcgttcggt tcagacgatg ctggcggaaa actgacctat actatagatt tcgccgccaa
     6601 gcagggacac ggcaaaatcg aacatttgaa atcgcccgaa ctcaatgtcg agcttgccac
     6661 cgcctatatc aagccggatg aaaaacgcca tgccgttatc agcggttccg tcctttacaa
     6721 ccaagacgag aaaggcagtt actccctcgg tatctttggc gggcaagccc aggaagttgc
     6781 cggcagcgcg gaagtggaaa ccgcaaacgg catacaccat atcggtcttg ccgccaagca
     6841 gtaaccattg tgaaaatgcc gtccgaacac aataatttac cgttcggacg gcatttttgc
     6901 gccataccgc cctaggacac gacggcacgg caggcaagct gatttcacta tataatgccg
     6961 tctgaaccaa cgagaaccat accatgcaag ctgattttaa ccgtcccgtc ctggccatcg
     7021 ataccggtac ttcctatttg tcgctcgcgc tgcgcgccga cggcgaaacc cgcctgttcc
     7081 atcaagaggc cggcagccgc caatccgaac tgattctgcc ggaaatccgc actctattcc
     7141 gaaatgcggg cattaccgcc gccgatttgg gggcgattgt ttatgcgaaa ggccccggcg
     7201 cgtttacggg actgcgtatc ggcatcggcg tggcgcaagg tttggcaacg ccgtttgata
     7261 cgcctttaat cggcatcccc acgctcgatg ccgccgcctc gctgccgccg ccgcaaagct
     7321 gcatactcgc cgctgcggac gcgcgtatgg gcgaagtgtt ttatgcgtgg ttcgacacgc
     7381 tgaaccgccg ccgtttgagc gattatcagg tcgggcgggc ggcagacatc cggctgccgg
     7441 agggatgtgc cttttcagac ggcataggca gcgcgttcgc gttggaagaa acgccgccgt
     7501 tttcaggcaa acccgatatg ccgaccgccg ccgacttcct caatctggcg gcggaaggcg
     7561 gttttcccgc cgtcgctgct gcacacgccg aactgctcta cgtccgcaac aaaatcgccc
     7621 taaccgccaa agaacaggcg gaacggaaag cccgaacatg aacatccgcc gcgcctcacc
     7681 gtccgactgc gccgcactcg ccgcgctcga tgccgtctgc aacccgtccg cgtggacgca
     7741 acgccaattt gagtccgcat tggtttcgcc gtccgaacag gttttcctcg cggaaaaaga
     7801 cggcgggatt gccgccttta tcgtttggca gaacctgccc gacgaatccg aactgcacct
     7861 gattgccacc gcgcccgaat gccgccgccg tggcgttgcg tccgccctgc tcgaatattg
     7921 gttcgccaac ctgcccgaag gcacgcaacg cctgctgctc gaagtccgcg caggcaacac
     7981 cgccgcacag gcactgtacg cggcgcacgg cttcagcatt gcgggcaggc ggaaaaacta
     8041 ttaccgtaca gccgacggta aaaccgaaga tgccgtcttg atggagaaaa tatgttaagc
     8101 gcgcgctacc tccacctgca cgaagccttg ggtttgggcc cgatgtggtt gaagcgggga
     8161 gccgttgtcc tgccgtcagc aacattaccg gaaagcccga cacaaatccg cccgcaaaag
     8221 caaaccgtcc tcagcattcc gcagcgtccg tccgaacagc ataccggtca ggcacggctc
     8281 gaaacgatga aagcgttgga aaccgccgcc gtacctacgc gcaaacccgc gcctgaaacc
     8341 gaaacgcctc tgtccggcgt ttcagacggc atcgcccccg ttcccgccgc ttcgggcata
     8401 accaaacttg ccgtcgtcag cctttgccca ccgattgagg atgcggttta cgggcaactg
     8461 ttctacggca aagcgggtgt tctgcttgac aacatactca aagccgtagg actggatgcc
     8521 gcctatgtcc acaaaacctg ttgggtgaaa accgccgccg tcggcaaccc gatgccgtct
     8581 gaagcggcaa tcgaaaccgc gacggtttcc gtcatgcagg aactcgacgg ctgccgcgcc
     8641 cccgccgtcc tattcctcgg acaggctttt gtccagcctg aacggcaaac gatgattgaa
     8701 actttgtgcg gcagccgtcc cttcttcatc atcgaccatc ccgcccggct gttgcgccaa
     8761 cccgaactta aagcccgcgc ctggcaggtg ttgaaacagt tgaaacgcgc cttgcggcaa
     8821 ggcggcggca gttgaagcgc accgcacggg gcggtagaat cgcaactgcg tcccaatatc
     8881 tgacagaaag cacaaaatga ccgatttccg ccaagatttc ctcaaattct ccctcgccca
     8941 aaatgttttg aaattcggcg aatttaccac caaagccgga cggcggtcgc cctatttctt
     9001 caatgccggc ctctttaacg acggcttgtc cacgctgcaa ctggcaaaat tttacgcaca
     9061 atccatcatt gaaagcggca tccgattcga tatgctgttc ggccccgcct acaaaggcat
     9121 tattttggcg gcggcaaccg cgatgatgct ggcggaaaaa ggcgtgaacg tcccgtttgc
     9181 ctacaaccgc aaagaagcca aagaccacgg cgaaggcggc gtgttggtcg gtgcgccgct
     9241 taaaggccgc gtgctgatta tcgacgacgt gatttccgcc ggcacatccg tacgcgaatc
     9301 gatcaagctg attgaagcgg agggcgcaac ccccgccggt gtcgccatcg cgctcgatcg
     9361 catggaaaaa ggcacgggtg agttgagcgc ggttcaggaa gtggaaaaac aatacggtct
     9421 gcccgtcgcc cccatcgcca gcctgaacga tttgtttatt ctgttgcaaa acaaccccga
     9481 attcggacag ttcctcgaac ccgtccgagc ctaccgtcgg cagtacggcg tagaataaaa
     9541 acaaagcata tgccgtccga accgccttat gcctcagacg gcatcaaacc tgacacacac
     9601 gaggaaatac catgcccgcc tgtttctgcc cccactgcaa aacccgtctc tgggtcaaag
     9661 aaacccaact caatgtcgcc caaggcttcg tcgtctgcca aaaatgcgaa ggactgttta
     9721 aagccaaaga ccatctggca agcacgaaag aacccatatt caacgatttg cccgaggctg
     9781 tttcggatgt caaactcgtt caccgtatcg gcacgcgcgc cattggcaag aaacagattt
     9841 cccgtgacga aatcgccggc atcctcaacg gcggtacaac ccagcccgat attccgcccg
     9901 caaccgccgc cacccctgct gccgcaccgc aggttaccgt accgcccgcc gcgcccgccc
     9961 gtcaggatgg gttcaactgg acgattgcaa ccctgtttgc ccttatcgtc ctcattatgc
    10021 agctttccta cctcgtcatc ctatgagcgc gcccgacctc tttgtcgccc acttccgcga
    10081 agccgtcccc tacatccgcc aaatgcgcgg caaaacgctg gtcgccggca tagacgaccg
    10141 cctgctcgaa ggtgatacct taaacaagct cgccgccgac atcgggcttt tgtcgcaact
    10201 gggcatcagg ctcgtcctca tccacggcgc gcgccacttc ctcgaccgcc acgccgccgc
    10261 tcaaggccgc acgccgcatt attgccgggg cttgcgcgtt accgacgaaa cctcgctcga
    10321 acaggcgcag cagtttgccg gcaccgtccg cagccgtttt gaagccgcat tgtgcggcag
    10381 cgtttttggg ttcgcgcgcg cgccttccgt cccgctcgta tcgggcaact tcctgaccgc
    10441 ccgtccgata ggtgtgattg acggaaccga tatggaatac gcgggcgtta tccgcaaaac
    10501 cgacaccgcc gccctccgtt tccaactcga cgcgggcaat atcgtctggc tgccgccgct
    10561 cggacattcc tacggcggca agaccttcta tctcgatatg cttcaaaccg ccgcctccgt
    10621 cgccgtctcg cttcaggccg aaaaactcgt ttacctgacc ctttcagacg gcatttcccg
    10681 ccccgacggc acgctcgccg aaaccctctc ggcacaggaa gcgcaatcgc tggcggaaca
    10741 cgccggcggc gaaacgcgac ggctgatttc gtccgccgtt gccgcgctcg aaggcggcgt
    10801 gcatcgcgtc caaatcctca acggagccgc cgacggcagc ctgctgcaag aactcttcac
    10861 ccgcaacggc atcggcacgt ccattgccaa agaagccttc gtctccatcc ggcaggcgca
    10921 cagcggcgac atcccgcaca ttgccgccct catccgcccg ctggaagaac agggcatcct
    10981 gctgcaccgc agccgcgaat acctcgaaaa ccacatttcc gaattttcca tcctcgaaca
    11041 cgacggcaac ctgtacggtt gcgccgccct gaaaaccttt gccgaagccg attgcggcga
    11101 aatcgcctgc cttgccgtct cgccgcaggc acaggacggc ggctacggcg aacgcctgct
    11161 tgcccacatt atcgataagg cgcgcggcat aggcataagc aggctgttcg ccctctccac
    11221 aaataccggc gaatggtttg ccgaacgcgg ctttcagacg gcatcggaag ccgagttgcc
    11281 cgaagcgcgg cgcaaagcct accgcaacaa cggacggaac tcgcatattc tggtgcgtcg
    11341 cctgcaccgc tgaccgcaac ggaaagccgc cgcagaaatg ccgtctgaac cccgtttcag
    11401 acggcatttc cccga
//
LOCUS       DAXHRI010000050        11172 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXHRI010000050 DAXHRI010000000
VERSION     DAXHRI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.050"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            143..1540
                     /gene="aspA"
                     /locus_tag="ACFM75_001007"
     CDS             143..1540
                     /gene="aspA"
                     /locus_tag="ACFM75_001007"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC6255572.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLAEKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            1553..1684
                     /locus_tag="ACFM75_001008"
     CDS             1553..1684
                     /locus_tag="ACFM75_001008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255573.1"
                     /translation="MPSEAGMLLQTAFSAFKAVLAAARFRRFVGKSQLFPFGKNPVY"
     gene            complement(1737..2612)
                     /locus_tag="ACFM75_001009"
     CDS             complement(1737..2612)
                     /locus_tag="ACFM75_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC6255574.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRAESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2614..3336)
                     /locus_tag="ACFM75_001010"
                     /pseudo
     CDS             complement(2614..3336)
                     /locus_tag="ACFM75_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(3359..3838)
                     /gene="ybaK"
                     /locus_tag="ACFM75_001011"
     CDS             complement(3359..3838)
                     /gene="ybaK"
                     /locus_tag="ACFM75_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC6255575.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3913..4275
                     /locus_tag="ACFM75_001012"
     CDS             3913..4275
                     /locus_tag="ACFM75_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6255576.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            4310..5173
                     /locus_tag="ACFM75_001013"
     CDS             4310..5173
                     /locus_tag="ACFM75_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC6255577.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPNLAFATQQDSDGICSIALPHGGILRIAPETALPPYDAAAESAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5271..6230
                     /gene="ttcA"
                     /locus_tag="ACFM75_001014"
     CDS             5271..6230
                     /gene="ttcA"
                     /locus_tag="ACFM75_001014"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC6255578.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(6324..9263)
                     /locus_tag="ACFM75_001015"
     CDS             complement(6324..9263)
                     /locus_tag="ACFM75_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC6255579.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPKLLFAA
                     KQYADNILKVQSQNGIGGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTRQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSPSMTDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLSKIHPAVSDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNIFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLLNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNETYINNGLMLSRRIDSIEDEDFIR
                     FNTMNYREFLEKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9276..>11172)
                     /locus_tag="ACFM75_001016"
     CDS             complement(9276..>11172)
                     /locus_tag="ACFM75_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6255580.1"
                     /translation="DLSDTAGANTEAIYTSDGITANSTQLEQLKKLFPACFDADGNFL
                     IDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQN
                     LLIRGDNLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELALLANID
                     EDEAARILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLC
                     DEVFGEGNFVANVIWQKKYSPQNDAKWFSNMHDYILCVAKNKDIWRPNLLERTEEQNA
                     RYSNPDNDERGNWKATDFSVKTYSENYDYPITTPSGRIVNPPPSRSWRTSKENFEKLL
                     ADNRVWFGINGNNVPSIKKFLTEVKQGITPSTILFRENVGDNQEAAKNIRDLFDIPPF
                     ETPKPVRLLNYLIKLSSNSDDIVMDFFAGSGTTAHAVMQLNAEEQNGSRRYICVQLPE
                     KTGEKSEARKAGYPTIFDITKARIEKAAAKIRAEHPDYTGDLGFKIFQTADNFRQHPD
                     KDFSPEQPDLPLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGSYTAYLCEKRLY
                     LMNAGFTSADLLAFIRKLDDDAAFNPNRVIVFGSNMASAMQHELDQAVRGYTNKKEIE
                     LNVIIRV"
BASE COUNT         2672 a         2990 c         2652 g         2858 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      181 cgagattccc gccgaagtat attggggcat ccacaccctg cgcgccattg aaaactttaa
      241 aatttccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      301 aaaggcgacg gcacaggcaa acggtaagtt gggcgcggtc aagcccgaaa tcgccgccgc
      361 cattgaaaag gcttgcgacg aagttctgct gaacaaccgc tgcctcgacc aattcccgtc
      421 cgatgtgtat cagggcggtg cgggaacttc ggttaatatg aacaccaacg aagtcatcgc
      481 caacctcgca ttggaagcct tgggatacga aaaaggccgc tacgacatcg tcaatccgat
      541 ggatcacgtc aatgccagcc aatcgaccaa cgatgcctat cccacgggct tccgccttgc
      601 cgtgtattac agcatcggcg aattgctcga caaactggcc gtcctgaaaa acgcctttgc
      661 cgccaaagcc gaagcgttta aagacgtttt gaaaatgggc cgcacccagc ttcaagatgc
      721 cgtgccaatg actacaggac aagaattcca atctttccaa gtgttgttgg aagaggaaat
      781 cctcaacctc gaccgcacgc gccaactgct tttagaagtc aatttgggcg caacggcaat
      841 cggcacgggc gtgaacacgc ccaaaggcta cgccgaactg gtggtcaaaa aactctccga
      901 agtcagcggc ttgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      961 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
     1021 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1081 cgaattacag gccggttctt ccattatgcc tgccaaagtc aatcccgtga ttcccgaagt
     1141 ggtcaaccaa gtctgcttca aagtcatcgg caacgacacc accatcactt tcgccgccga
     1201 agcaggtcag ttgcaattaa acgtaatgga gccggtcatc gcccaatgta tgtttgaaac
     1261 gatttccctc ttgggcaatg ctgcggtcaa cctcgccgaa aaatgcgtca aaggcattac
     1321 ggtcaaccgc gaaatctgcg aacgttacgt tttcaactcc atcggtttgg tcacttatct
     1381 gaatccgtac atcggccacc acaacggcga cttggtcggc aaaatctgcg cccagaccgg
     1441 caaaggcgta cgcgaggttg ttttggaacg cggcctgttg agcgaagaag aactcaaccg
     1501 catcctctct cctgaaaacc tgatgaaccc ccatctgtaa cgttgcaggc aaatgccgtc
     1561 tgaagcaggg atgctgcttc agacggcatt ttcggcattc aaggctgttt tagccgccgc
     1621 ccgattccgg cggtttgtcg gaaaaagcca gttatttccg ttcgggaaaa atccagttta
     1681 ttgacaaaaa aacaattact taaaacgcgt ttgcgcggta tgcggacacg gcgggattat
     1741 atgccgaaca gtgccttgac ctccgcccaa taaaacaggc aggcgatgga acacagtgcc
     1801 aatacgattc ccgccgtatt gattgtgttg attttttcct taaaaaacag tgcgcccgaa
     1861 agcgtaccca aaacaatcac accgatattc ataccggcaa aaaccaaggt cggattgtcc
     1921 ttcatcattt ggtgcgcggt gatgtaggtt acgatattca taaaattcaa accgcccaag
     1981 agtatgccgc cgaccacact ctctgcgcgc catctgaccg atttggcaaa caggcaggca
     2041 aacatcagca cacccgccag cacaaatgca accagcaggt tgcccgcaaa tgccgttccg
     2101 cttttggcaa gctgtttgaa caggatatcg ataatgccgt aacctgccca cacgcccagc
     2161 agcaatgccg cctgccgcca cgcgcttcct gattttttgc caccgctgtg tttccaaagc
     2221 aggcagaaca gcgcggcaaa ggcgaggcac agcccgatta gtttgccttc gctgagtttt
     2281 tcgccaaaca gcgttaaagc ggcaacaatc ggcaaaaaca gcgacaaacg ctgcgccgcg
     2341 tcggatttga cgataccggc ggattctacg gatttgccca ttatcacgaa tacggacggc
     2401 agcagcacgc ctaatgcggc aaacagcggc cacgtcggca aaaatgcgcc gatattgccg
     2461 acatccggct tcaataccag cagggtcagt atgaccgcga ccacataatt gacggcgacc
     2521 gcctgcgcga tgtcgatttt tttcttcctt gccattttca gcaaaatgga aaccgacacg
     2581 ctgaacacga tgctgattaa caggtaagcc atactatatc catttctcgg gtgcaaccgc
     2641 cctgccgctg ttgaagcgga taaagccgac gatgatgcgg tagaaatacc agacgttcgt
     2701 tgcaataata atcagcacgc cgatacccaa aagggcagtc agcgcgccca aaagataaag
     2761 ccaaaatgtg cgccaaaagg ttttaatcag gtattcggta tgtgtggcat agacaatgct
     2821 gtccaaactg ttccgtttca tataggcgag aatcacgccg actatgggca tataggcgaa
     2881 aatcacgcca actatgggca tgaacagcat gattgcaccc aaaccgaaca ggatgtaggc
     2941 ggcgaggaca tagttgcgcc cgcttccgcc gccggacggt tcgtaagcat ggcgcgaacg
     3001 tgaggcctgc cgttcaaacg cctgtctgaa cgcctgttcg cggcgcatct gttcgcggta
     3061 aaactgctcc ctgcgcgcct attcgcggcg gaatgcctct tcctgccgcc cgcgttcctc
     3121 atgccgtctg aaggacgcgt cgtattgcat ccgtttcgac aggtcggaaa gcgtatcgta
     3181 agcccgttgg atttctttga acttttcttc cgcctgcgga ttgcccggat tgcggtcggg
     3241 atggtatttc atcgccagct tgcggtaggc gcgtttgatt tcgtccgctc ccgcctgcgg
     3301 cgacacgccc aatacggcat acaaatcttt gtccatattg ttcctttata ctttcccgtc
     3361 aaaccgccgc ctgtattgtt ttcgggttca aaatatttaa atctccggga cggatgccga
     3421 taatgaaccc gcgttttccg ccgttgatat agatggtttc caaatccatc accgactgtt
     3481 cgacgtaaat atccaacttt gtccggatgc cgaacggcgt tgtgccgccg accagatagc
     3541 ccgtccactt gtttgcctgt gcgggcgtag cgggttcgat gtgtttcgca cccaaatgcc
     3601 gagccagatt gcgcgtggaa atctgtttgt cgccgtgcat caagacaatc agccccttac
     3661 cgttttcatc ttgcaaaaca atggttttaa tgaccaagtg ttcgtctttg ccgaatagtc
     3721 gggcaaactg cgccgtgccg ccgtgttcct cataggcata ggtaaaaggt tcaaattcga
     3781 tgccgttttc acgcaaaacg cgcacggcag gtgttatcgg ataagtgtgt ttactcattt
     3841 ttcgtcatgc aaaacatgga aatcggccaa gcgcaatgtt accatatatc ccctttatcg
     3901 aaggaaaccg atatggatat ccgatatttc ggcacaacgc cccgctattc cgaagccgtc
     3961 ggcgcaaacg gcttgatttt cctctccggc atggttcccg aaaacggtga aacggctgcc
     4021 gaacagactg ccgacgtgct tgcccaaatc gaccgctggc tggcggaatg cggctcggac
     4081 aaagcgcacg ttttggatgc ggtcatctat ctgcgcgata tgggcgacta cgcggaaatg
     4141 aacggcgtgt gggacgcttg ggttgccgcc ggcagaacgc ccgcccgcgc ctgcgtggaa
     4201 gcgcggctcg cccgtccgga gtggcgcgtc gaaatcaaaa taaccgccgt gaagagagac
     4261 gcggcaactg cataaacaga tttcctttat atttcagagt acgcaaatga tgaaaacctt
     4321 actgcccttt ttcggcgtgg tgcgcgtcag cggcgaagac cgccaaacct tcctgcacgg
     4381 acagctttcc aacgacatca accaccttca gaccgggcag gcgtgttatg cgacttacaa
     4441 cacgcccaaa ggccgcgtca tcgccaatat gattgtcgtc aaccgaggcg gcgatttact
     4501 gctgattatg gcgcaagacc tgcttgaagc aaccgtcaaa cgcctgcgga tgtttgtcct
     4561 gcgtgccaaa gccgtttttg aaatccttga agattacgcc gtcggcgcgg aattggaagc
     4621 atccgccgaa ccgcttgccg cgcaagaacc caaccttgcc tttgccacac aacaagattc
     4681 agacggcatc tgctctatcg ctctgcctca tggcggcatc cttcgcatcg cacccgaaac
     4741 cgccctgccc ccttatgatg ccgctgccga aagcgcgtgg cggctgcacg aaatccgcag
     4801 cggttatccg tggatatgcg cggctaccaa agaaaccgcc gtcgcccaaa tgctcaacca
     4861 gcacatcatc ggcggcgtac acttcaaaaa aggctgctat ccgggacagg aaatcattgc
     4921 ccgtgcccag tatcgggggc aggtcaaacg cggcttggcg gtattgtcgg gaaattcggc
     4981 cgtcgaagca ggaaccctgc tgacggcgga cggcgaagag gccggcatcg tactcgacag
     5041 cgttcaagat tcggaaaact ttaccgccct taccgtgatt aaattctccg ccgcacaaaa
     5101 agaactgacc gccccaaacg gcagtatctt caaagcggtt catcccttct ttaaaaccga
     5161 aaatgcagaa taatccctct aatgccgtct gaaccttttt cagacggcat tttaaaaccg
     5221 cccctgtttt cagttacaat gccgcatccc caatttttca aaccaccgcc atgtccaaaa
     5281 aaaccaaaca agaactcgaa aacaacaaac tcagcaaacg cctgcgccac gccgtcggcg
     5341 acgcgattaa cgatttcaat atgatcgagc cggacgacaa aatcatggtc tgcctctccg
     5401 gcggcaaaga cagctatgcc ctgttagaca tcctgcgcca gctccaagcc agcgcgccga
     5461 ttgatttcca actggttgcc gtcaatctcg accaaaagca gccgggcttc cccgaagaag
     5521 tattgccgac ctatctcgaa agcatcggcg ttccctacaa aatcgttgaa gaagacacct
     5581 actccaccgt caaacgcgta ttagacgaag gcaaaacgac ttgttcgctg tgcagccgcc
     5641 tgcgccgcgg catcctctac cgcactgcaa aagaattggg ctgcaccaaa atcgccttgg
     5701 gacaccaccg cgacgacatc ctcgccacct tgtttttaaa tatgttttac ggcggcaaac
     5761 tcaaagccat gccgcccaag ctggtgagcg acaacggcga acacatcgtc atccgcccgc
     5821 tggcgtatgt gaaagaaaaa gacttaatca aatacgccga attaaaacaa ttccccatca
     5881 tcccgtgcaa cctctgcggc tcgcaaccca acctgcaacg ccaagtcatc ggcgatatgc
     5941 tgcgcgattg ggacaaacgc ttccccggcc gcatcgaatc gatgttctcc gccctgcaaa
     6001 acgtcgtccc ttcccacctt gccgataccg aactcttcga ctttgtcgga ttggaacgcg
     6061 gtcaaagcct gaaacacggc ggcgatttgg cgtttgacag cgaaaaaatg cccgaacgtt
     6121 tttcagacgg cagcgaagaa gacgaaagcg aaatcaaaat cgagccgcaa aaagccgaac
     6181 gcaaagtcat caacattctg gctaacaagc cgaaaacctg cggcgcgtaa ccccccgccg
     6241 tcaaaaatgc cgtctgaagc ccgtttggcg tttcagacgg cattttaaaa tcccgaagca
     6301 gacggcttcc aaacatttgt ttctcagtgt ccgttttcag aatgcccgcc tgcgggcata
     6361 ttttgcccga tcagttcggc tatcctctcg ccgtcaaact gcgttttgaa caccaccttg
     6421 atttctttgg aaatctcggc aaacagcttt tcggcatgtt tgattttccg ctcttcgctt
     6481 tttcgcaaat cttccgcccc gtcagtccct ttggcttcaa tcacaaagtt caggatctcg
     6541 ccgcttttgg ttttcacgat ataggcaaaa tcgggcgaat acgtgccgcc gccggcaaca
     6601 gggattttga tggagtttct cggtattttg gtaaatacgg ttacgccttc aatttggttg
     6661 ttggcgacat tttcatgttc tatatccgaa tcgtagaaaa tctcgtcgaa gagatagccg
     6721 gcggcaggcc ggtgctccgt atcttcaaat ctgcccaaat ctgctttttt caccgcgcgc
     6781 ggtttgccgt ctttattggt aaatttggtc ggatggattt tgctgccgac aagccggtaa
     6841 tccagttcga atttatggaa ggaatgatga agcaaaaacc ggttgaagcc gtttttgatt
     6901 tgggcgatgg tctgcatatt caaaaaatcg ccaatgttca gttcgtcgcg gacgcggtaa
     6961 aacgcctgat gcaaagtctg catgcggatt tttgccgttt gtgccagttt ttccagaaat
     7021 tctcggtaat tcattgtgtt gaaacggata aaatcttcat cttcaatact gtctatgcgg
     7081 cgggaaagca tgagcccgtt gttgatataa gtttcgttta ccgccgtgcg tatgcctgcc
     7141 tgcgggaatt tggcggcgtt ttcacgcaaa taggcggtaa ataaatcgac aaattcggct
     7201 tcatccttga ttttgtactg caaaacggct ttatggtgaa tcagctccca caaggctttg
     7261 agttcttcat atttgccttc gcgcatgatg atggtgtctt tgccctcgtc tttggcgttg
     7321 ctgactttgc ctttgtccaa acctttgggg aaggcttcgg gataggcggc ttttaatttg
     7381 tcatagccgt cttcggcaaa gttttcattg tcgtcaatga tgccgtctga aaacagttgg
     7441 tttaacaata ccagcggttt gatatcgggg tatttttgca atattttttg tttcagctct
     7501 tcggtaaact ttttggagat ttcttcctga aaagaattgt cgttgatttc gccgacaagc
     7561 tgcttcacaa agtctttttc gctgctatcg acaaaataat tcagtttgta cggcacatcg
     7621 cgcacccgcg ccatcagctc gtttaccggc aggcgcaggc cgcgtccaac ttcttgcagc
     7681 ttggaagtcg tgctgccgct ggaacgcagt ttgcaaatct ggaaaatgtt gggattgtcc
     7741 cagccttcac gcagcgtcca tttggaaaaa ataaagcggc gcgggttgtc caaagacaac
     7801 agtttttcct tatcgtgcag gatttcattg atttcctgct cgattctatc gtcgctgtct
     7861 gtattgtctt tggaaaaata accgccgtgg caggcggata catcgtccaa cgtcttttgc
     7921 aggtaatcac gataaaacgg gtcgctttcc gttttcaggc ggcgtgccgc ttccgcacgg
     7981 atccagcttt caaatttatc tttcaggctg cctgaaagct cgttgccgct gcggtagccc
     8041 gcgatatcgt caataaaaaa cagcgtcagc ggcttgattt tgggctgtgg cgcgcgttct
     8101 gccaaaagcg cgcgttccag cttgaaatgt tcggcaaccg ccctctgcat catcgcgtcc
     8161 tgcaccgttt gcgaatagga ataagggttg atgacggcac ccgttttcaa ctccaagccg
     8221 ttgcttaaca ccaccacggt tttattcatt ttgtcgattt tcaaatccga aacagcggga
     8281 tggattttcg acaaatcttc gccttttgcc agtttgaacg tctgcttttt gtccttttcg
     8341 tttaattcaa atttcgcttc tttgccgtcc gacgacacca gttttaccgc cgcatccatg
     8401 ccgccctgca tttcttcctg aaacacgcgc acgcctttga ccagcccgtc gttaaatgcg
     8461 tctactgccg tcaaacggta aagcaagttg taatattcat cgttaaatgt cgcaccgtag
     8521 cgcaaaatat attgcggttt taagcgtttg atattgcccc acgttttcgc gctctcccgg
     8581 gtcgggaatt tatgcggttc gtccacaatc acaaacgggc gcacggcggc caatgcatca
     8641 acgggattgt caaacaaatc cttcaatgcc ttgtcgcccg tatcggtcat ggacggcgaa
     8701 ttaaccatgc ccgcgttaat cagcagcaca tgaatttcct ttttattttc cgctttgaca
     8761 aattgctcaa tcgttatggg cgcattggac tttttgccct tattcttttt cgcgctttct
     8821 accacatagg ttttcaggcg tacgccttca taatctccgc caaaatcctg ttcaaaatgc
     8881 tctgccaaag ccttgctttg caaaaactgc cgcgttcccg ccttaatgga caaagtcggc
     8941 acgaccacga taaatttgaa cacgcccagc caacggtgca gctcgaacat ggtttgtgtg
     9001 taggtatagg ttttgcccgt gcccgtttcc atggaaatat caaggatatt ttggtcgtcc
     9061 gaacggtcgg ggaatcggcc gcctatgccg ttttggcttt gcacttttag gatattgtct
     9121 gcgtattgtt ttgcagcaaa caaaagtttg ggattttcgt ctgccgcccg atatttgggc
     9181 gttgccccgt caaacacgcc caaaaccgcc gaaaccgccc gcatttggtg cggctggttt
     9241 ttctcgtaat taaaaccgct cataaacttt ctccgtcaaa ccctgataat cacgtttaac
     9301 tcaatctctt ttttattggt ataaccgcgt accgcctggt caagttcgtg ctgcattgcg
     9361 cttgccatat tgctgccgaa tacaatcacg cggttgggat tgaaagccgc atcgtcgtcc
     9421 agcttgcgga taaacgccaa caaatcggcg gaagtaaaac cggcattcat cagatacagc
     9481 cgtttttcgc acaaatacgc cgtataactg cccaaccgca caggctcaac cggcgtggtc
     9541 agtgccgctc cgtcatacag cgtccaggtg gtcagaagcg tttgcagctg ttcttcgctt
     9601 aattcatcgt taagcggcaa atccggttgc tcgggcgaaa aatccttgtc cggatgctgt
     9661 ctgaaattgt ctgccgtttg aaagattttg aagcccaaat cgcccgtgta atcgggatgt
     9721 tcggcgcgga ttttggcggc ggctttttct atgcgggctt tggtgatgtc gaagatggtc
     9781 gggtagcctg ctttacgggc ttcggatttt tcaccggttt tttcaggcag ttgcacgcag
     9841 atatagcggc ggctgccgtt ttgttcttcg gcgttaagct gcatcacggc gtgtgcggtc
     9901 gtgccgctgc ctgcgaagaa gtccatcacg atatcgtccg aatttgaact aagtttaata
     9961 agataattta ataacctaac aggttttgga gtttcaaaag gtggaatatc aaataagtct
    10021 ctaatatttt ttgcagcttc ctgattatca ccaacatttt ctctaaataa tattgttgaa
    10081 ggtgttattc cttgttttac ttctgttaag aattttttaa ttgagggaac attattacca
    10141 tttattccaa accaaactct gttatctgct aataattttt caaaattttc ttttgaagtt
    10201 ctccaacttc tactaggtgg cggattaaca attcgtcctg atggcgtggt aattggatag
    10261 tcataatttt ctgaatatgt tttcacagaa aaatcagttg ctttccagtt tccacgttca
    10321 tcattatcag gattggaata tcttgcattt tgttcttctg ttctctctaa taagtttggt
    10381 cgccaaatat ctttattttt tgcaacgcat aaaatgtagt cgtgcatatt tgaaaaccat
    10441 tttgcatcgt tttgtgggct atatttcttt tgccaaatca cattagcaac aaaattccct
    10501 tccccaaaca cttcatcaca caacaatttc aactgcgccg cttcgttatc gtcaatcgag
    10561 ataaaaatca cgccgtcgtc ctttaacagt tcgcgggcga tatacaggcg cggatacata
    10621 aaggtcagcc acgcgctgtg cgagtttgag cctttgtcgg tgaaatctaa aatccgcgcg
    10681 gcttcgtctt cgtcaatatt cgccaggagg gcaagttcgg cgggtgtgaa tttgcggtcg
    10741 tcctgataga caaagccgtc cgagccagtg ttgtagggcg ggtcgatgta aatcatcttc
    10801 acgctgtttg tataggcgtt ttttaagtgt ttcaacactt ctagattgtc gccacgaatc
    10861 agcaggtttt ggctgcctgc attttcgggc ttggcgttgt gcgtcttatc ttcacttatc
    10921 agggtttcgg gcggcaggtt gcgaagcagg cgggcatatg atttgcccag ccagttcatt
    10981 tcgtaaaatt cgcgtccgat gtcggtctgt ggcgcaattt cggcttgtaa tctgtcgata
    11041 aggaaatttc cgtctgcgtc aaaacaggcg ggaaacagtt ttttgagctg ttcgagttgg
    11101 gtagagttgg cggtaatgcc gtctgaagtg tagattgcct cggtgttcgc cccggctgtg
    11161 tcgcttagat ca
//
LOCUS       DAXHRI010000051        11164 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXHRI010000051 DAXHRI010000000
VERSION     DAXHRI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.051"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            62..1015
                     /locus_tag="ACFM75_001017"
     CDS             62..1015
                     /locus_tag="ACFM75_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC6255581.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIIFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1126..1449)
                     /locus_tag="ACFM75_001018"
     CDS             complement(1126..1449)
                     /locus_tag="ACFM75_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255582.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1596..2957)
                     /locus_tag="ACFM75_001019"
     CDS             complement(1596..2957)
                     /locus_tag="ACFM75_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC6255583.1"
                     /translation="MLKKWLNKMLPSSRSSKEAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKEAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASAEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            complement(3311..5011)
                     /gene="recJ"
                     /locus_tag="ACFM75_001020"
     CDS             complement(3311..5011)
                     /gene="recJ"
                     /locus_tag="ACFM75_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC6255584.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRDVQSPAELDD
                     KLAALLPYQSLTNCEAAAGRLADAIERQEKILIVADYDADGATACSVGMSGLAAMDAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDAEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTALAVYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLKACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(5080..5793)
                     /locus_tag="ACFM75_001021"
     CDS             complement(5080..5793)
                     /locus_tag="ACFM75_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255585.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTDDLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAAG
                     AVLVLPMAALIAAANSSDK"
     gene            complement(5840..6346)
                     /gene="yjgA"
                     /locus_tag="ACFM75_001022"
     CDS             complement(5840..6346)
                     /gene="yjgA"
                     /locus_tag="ACFM75_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC6255586.1"
                     /translation="MFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLDAD
                     LYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     QARVRLLADDGALTQFMSDFPHADAGKLRTLVRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            complement(6378..6509)
                     /locus_tag="ACFM75_001023"
     CDS             complement(6378..6509)
                     /locus_tag="ACFM75_001023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255587.1"
                     /translation="MNSIEIFLDDALRHFNRFGRQGQKRAVCRADGAKCRLNAAAFC"
     gene            6499..7830
                     /gene="pmbA"
                     /locus_tag="ACFM75_001024"
     CDS             6499..7830
                     /gene="pmbA"
                     /locus_tag="ACFM75_001024"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC6255588.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8127..8330)
                     /locus_tag="ACFM75_001025"
     CDS             complement(8127..8330)
                     /locus_tag="ACFM75_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC6255589.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8573..8875
                     /gene="clpS"
                     /locus_tag="ACFM75_001026"
     CDS             8573..8875
                     /gene="clpS"
                     /locus_tag="ACFM75_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC6255590.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8877..>11164
                     /gene="clpA"
                     /locus_tag="ACFM75_001027"
     CDS             8877..>11164
                     /gene="clpA"
                     /locus_tag="ACFM75_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC6255591.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVHNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDEDEDDGNY
                     SSDGMDDDNGNRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRK
                     NNPLLVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEA
                     RVKSVLKQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATT
                     YDEYRTIFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAA
                     AELSARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEK
                     TVSHDDKQVLQFLGRDLKNMVYGQENAIDTLVAAVKMSRSGLALPDKPIGSFLFSGPT
                     GVGKTEAAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNK
                     QPHCVLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLS
                     RPSLGFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEH
                     QLLDKKVEAEFTPALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGG
                     FVRIDWDAAKEEAVLKFKKSKV"
BASE COUNT         2661 a         3252 c         2821 g         2430 t
ORIGIN      
        1 ggtttaaatc taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca
       61 tatgacacat accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct
      121 gtgcggttct tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag
      181 ccgccgtttt gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact
      241 gctcaaactc ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc
      301 cgccgtcgaa gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta
      361 ttatttccgc accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat
      421 ccgcgcccta ctcaaccacg acatcatatt cggtctcggg ccggcaggta cgggcaaaac
      481 ctatctcgca gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat
      541 tttagtgcgg cctgccgtcg aagccggcga gaaactgggc ttcctgcccg gagacctgac
      601 ccagaaagtc gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt
      661 tgaccgtgta accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat
      721 gcgcggcagg acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc
      781 cgaacaaatg aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg
      841 cgacaccagc caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga
      901 gaaactacac ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg
      961 gcatcctttg gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc
     1021 caatgccgtc tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc
     1081 aagctccgta tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct
     1141 gcatcggatt gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg
     1201 cactttctgc cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg
     1261 cgtaacgctg catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt
     1321 gccgccgctt tccaatacgg cgacacggtg ttccaactgc gctagacgtg cttccaaatc
     1381 gccgattctg tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca
     1441 ttttttcatc aatttttcct tgttgcttga accctgccca cccgacatcc tttttccgag
     1501 aggcagggtc agactttatt tacggggatg aatcccctta caaatcagga taatacatag
     1561 aacggcactt tatgcttcat cttgtctgtt gacccttatt cccaatccgt tccctcaacc
     1621 ttcggcttgg gtttgcgcct gcgacggcgg cgttttttcg cctgtccttc gtttttttcg
     1681 tgtcgggcgg cggcctcgtt tttggtcatc tccgaccgct gttccgccga tgccgtctga
     1741 aacgccgtcc accactcggc aagggcgcgg tccgcattgc cggtttcggc gcgcaagagc
     1801 aggaaatcat aggcggcacg gaaacgcgcc tgtgcaaaca gtttgtgcgg acgtgcgcct
     1861 ttgcggtttt caaactgcgg ctggaacatc caaatttcgc gcatcgtggc ggaaaagcgt
     1921 tgcggcacgc cccaaccgcg ttcgacggtt tcgcgcatcg tattgattgc atcggacagg
     1981 gcgggcgcgg gtttcaaacc ctgttgcaga ttgcttttcc aatggcgttc caactcgggc
     2041 cacatcagag ccgccaatac aaaaccgacc gaaaccgatt tgtcggcacg cagccgctca
     2101 tcggtatttt tcagggcgag caccgtcatt tttccggcga tgccgtctga aacgcgcaag
     2161 gcattgagca gcggatggat gtcgtccggt atgtcaaatc cgttcaaacg ttttagacac
     2221 tcgcgcgcgt gccctgaaaa cagcagtttc ataatttcgt cgaacagcct tgccacaggt
     2281 tcgtgcttca gacggcatat tgattcggca atcggtgcgg cggtttcttc cgacagatca
     2341 aatcccaatt tgcccgacag gcggatggcg cgcaaaatcc tgacagggtc ttcctgatag
     2401 cgttcggcgg catcgccaat cataaccagc ctgcgggcgg caacatcggc aatcccgttg
     2461 tggaaatcca aaatctcttc tttttcagga tcgtaataca aggcattgca ggtaaaatcg
     2521 cgccgcatcg catcttcttc gatgctgccg taggtgttgt ccttcatgat tctgccgtgc
     2581 gcgttctgat gaaccttcgc accgccgcga aacgtcgtta cttcgataat ctccgcaccg
     2641 ttcatcacat ggacgatttg aaaacgcctg ccgatgatgc ggctgcggcg gaagagtttg
     2701 tgcacctgtt cgggcgtggc atcggttgcg acatcgaaat ccttgggttc gatgccgagc
     2761 agcaggtccc tgaccgcgcc gccgaccaca taagcctgaa accctgcctc tttcaggcgg
     2821 cgtatgacgt tttcggcggc aaagctcaac atttcggcac ggatgttgtg tctttcggca
     2881 ggaatgaccg ttttactttc cgcttcttta ctgctccgac tggaaggcag catcttattc
     2941 aaccattttt tcagcataaa tattctgacc cgaagagcgg catctccacg ggcaaccgtg
     3001 ttcagacggc atcaggcgcg gtttgcaaga tgccgtaacc ataaacaaaa ggcgtagaaa
     3061 ctacgccgtc cgaatcacgc cgccctcgcg gcggcaacgc gtcattataa cagattgccc
     3121 tccaaaaaaa tcagaccctg cttttgtggc agagtctgaa atttgatgcg ccttatatat
     3181 tgcgtcccta agaagggatg attaacaaaa attaacgccc tttactttct tttccgattg
     3241 ggaacattcc cgtcgtacaa acaggcaaat gaaggaacag aaatcacatt caaacggcgt
     3301 tccgccgcct ctacaccgct tcccagtaat cgatatacag ctgcaattcg agattgttgc
     3361 gccattcgtt ggcaacgggg cggtaaaccg tgcggatgta ttcggggatg tcttcgctgc
     3421 aacgccaaaa catggcttca aattcgcagc cgtctttttg cagccacgct tttttgtgtt
     3481 taccctccgc gcccaaaggc tgctggcgga cgacgtggaa ctcgtcggta aagcttggcg
     3541 gcgcgaagcc ctgcccccaa acgtgtcggg caaggttttg agcctgttcc agcgtgatgt
     3601 cgcaggcttt caggctgccg tcggtgatga aggtttgcga caaatcgtct tcgcacacca
     3661 tctcgcgcac ggcttcttca aaggccgtct gaaacgcggg gatattgtgt tcgagtatgc
     3721 tcaagcccgc cgccatcgcg tgtccgccga atttcaaaat caaatcggga tggcgtttgg
     3781 acaccaagtc caaagcatcg cgcaggtgca ggttgggaat ggaacgcccc gaaccgcgca
     3841 cttcgccgtt gtcggcaggc gcaaacacga tggtcggacg ataaaaacgg tctttgaggc
     3901 ggctggcaac gatgccgacc acgccttgat ggaagtcgtc gcgatacacc gccaaagccg
     3961 tctgacctga aggcagggtt tcaggaaaag cattcagcgc gtcttgcagc atagattgct
     4021 cgatttcgcg acgctcgata ttgaggttgt ttaactgagc cgccagttcc tgcgcctcgg
     4081 catcatctcg cgccaacagg caggcgatgc cgaccgacat atcgtccagc cgtccggcgg
     4141 cgttgatgcg cgggcccaac gcaaaaccca tatcaaacgg ctgcgccttg cgccagtcgc
     4201 gccgcgccac ttcaaacaag gcgcggatac cggggcgcat tttgccggag cgcatccgtt
     4261 tcaaaccttg cgacacgagg atgcggttgt tgtggtcgag agggacgaca tcggcaaccg
     4321 tgccgagtgc gaccaaatcc aaaagttcgc ccaaattcgg ctctttgatg ccgtctgaaa
     4381 aataattgcg gcggcgcaat tcggcacgca acgccatcaa tacataaaaa atcacgccca
     4441 cgcccgccaa gcttttgctt ggaaaaccgc agcctttttg gttcggattg acgatgatgc
     4501 agtcgggcac ggtctcggcc ggcaagtggt ggtcagtaac gatgacatcc aaacccagag
     4561 cctgtgcgcg cgccacgcct gcgatgctgg caatgccgtt atcgaccgta atcagcaaat
     4621 ctacaccttg ctctgccgcg atttcagcca gctcgggcgt taagccgtag ccgtgttcaa
     4681 agcggttggg cacgaggaaa tccactttcg cgtccatcgc cgccaaaccg ctcatgccga
     4741 cggagcaagc ggtcgcgccg tcggcatcgt agtcggcgac aatcaggatt ttttcctgac
     4801 gctcaatcgc atccgccaaa cggccggcgg cggcttcgca gtttgtcagc gattgatacg
     4861 gcaaaagcgc agcaagcttg tcgtccaact cggccggact ttgcacatca cgcgaagcac
     4921 aaagccgggc gattaaagga tcggcaccgg cggtgagcaa atgattaaaa acgtcggtat
     4981 tgacggagcg ggtttggatt ttgacggaca taacgtggaa ctttacagtg cgggtgtgct
     5041 tgaaatgccg tctgaaggtt ttgcagacgg cattgccgtt catttgtctg aggaattcgc
     5101 ggctgcaatc aacgccgcca taggcaagac cagtaccgca cccgccgcat ccagtatcaa
     5161 cgtggtgggc gtatatgcaa tattttcaaa caacttggat ttgtcggtat gttttttcgt
     5221 aaccgtgtaa taaatatcgg caggcacact ttgctcaaaa tgataatcgg cgttcagttt
     5281 ttgcggtgtg gcgtagtatt tgcctttggc ggagacgcag cgcgtgtaaa tggtccgatt
     5341 gtcgagttcg accgcttcaa actcaagctg tttcagcttg gcgatgtcgg caggtctgtc
     5401 ggtatcgtag cgcaggcaaa ggtcgtcggt actgaaattc tggctggcgg gcgattcgag
     5461 tttgaccggc agggcttggt aggcaaagcg cgggttgggc tcaaccattt gaaactgctt
     5521 gtccaacccg gccttcaaaa tgcccgtcag cttcgccgaa tcttcaggat tgacgacgaa
     5581 ccagtatttc ccgcccatca tcaccaggct gcccttttcc aattgggcat tgtctttggc
     5641 aaccacaccg aaggcgcgga tttggtcttt gtcaacgtgt ttataggcgg tcgtttggct
     5701 gaacgggtcg ttcatgcccc acatcattgc cgtacagcca ttcagcatca aggttgccgc
     5761 caaaacggcg gcggtcgttt tacggaacaa cattttatcc tccaatctaa ataacggcgg
     5821 atgtcgtctg aaaatatgcc taaatttccg cgtccccgtt ttccatcacg gttttcaatt
     5881 cttgaaacag cgcgcggaag tttttcggcg gtttgttttg ctcctgctct tttttggtgt
     5941 tgcggacgag cgtcctcagc ttgcccgcgt ccgcatgcgg aaaatccgac ataaactgcg
     6001 tcaacgcgcc gtcgtctgcc aacagccgta cgcgcgcctg ttccacgcgt tgcaaaaagg
     6061 cgttgtgcgc cgcatcgtcg ccgcgcagct tggcaagaaa cgcctcgatg ggcgcgggat
     6121 cggtatcgcg catcagcctg ccgataaatt gtgcctggcg tttgagcgcg ccgttggatg
     6181 tgattttttt ataggcggtt accgcctcgt acaaatccgc atccaaaccg atttttttca
     6241 gcgtatcgtt tgagagcttg gtcaacgcca tacccaaatc ctgcaaatcg ttcatctgct
     6301 ttttcatttg ggttttgctg atccattcgt cttcttgttc aaacatcttg attctcgaat
     6361 ttttccaata gcgcatttta acagaatgcc gccgcgttca gacggcattt tgcaccgtcc
     6421 gccctgcaaa cggcgcgctt ttgcccctgc cgcccgaaac ggttaaaatg ccgcaaagca
     6481 tcatcaagaa agatttctat gctgttcaac cacaccgctt ccgaactcct cgacctctgc
     6541 cgccgcacgc tcgacttggc aaaagcgacg ggcgcaaccg ccgccgaagc cgatttcagc
     6601 gaatcattgg gacaaagcgt cagcgtgcgg ctgggcgaaa tcgaacaaat cgagttccag
     6661 caggacaagt cgctggacat taccgtttac gtcggcaaac gcaaaggccg cgccagtact
     6721 gccgacttct ccgaaaaagc cctgcaagac accgtcaaag ccgccatcga catcgcccgc
     6781 cacaccgccg aagacggttg cgccggactt gccgatgcct gtctgatggc aaaacacatc
     6841 ggcgaccccg acctttacca cgaatgggat ttggatacgg aagccgccgt cggcttggca
     6901 aaacaatgcg aacaagccgc cctgaacgag gatgagcgca tcgaaaactc cgaaggcgcg
     6961 gcggtgcaaa ccggccatta ccaatacgtt tacggcaaca cccacggttt tgccgcacac
     7021 cggcaaggca cgcaccacag catttcctgc agcgtcgttg ccgccgacga aaacggcatg
     7081 cagcgcgact actggtacga ttccgcctgc cgccatccgg atatggacag ccccgaaacc
     7141 atcggtcaaa ccgccgcccg ccgcactttg cgccgactcg gcagccgcag catcccgaca
     7201 ggcagctacc ccgtcctctt cgataccacc gtttcgggcg gtcttatcgg acacctcgtc
     7261 ggcgcactct ccggcggcgc gctctaccgc caaagcagtt tcctgatcga cagcatcggc
     7321 aaaaaagtcc tgcccgattt cctcaacctg cgcgaagaac cgcacatccc ccgctctttc
     7381 cgcagcagct attttgatgc ggaaggcgtt gccaccgcac cgcgcttcgt gattcaaaac
     7441 ggcattgtcg aaggctattt cctcagcagt tacagcgcgc gcaaactcgg tatgcagacc
     7501 acgggcaacg ccggcggcgc gcacaacctg tatttgaacc atacgcacga aacgcaatcc
     7561 gacctgctga aagaaatggg gacgggattg ttggtaaccg aactgatggg acagggcgcg
     7621 aacaccatta ccggtgacta ctcgcgcggc gcggcgggtt tttgggtgga aaacggcgtg
     7681 attgcctacc ccgtccacga gattaccgta gccggacgct tgcaggatat gtaccgcgac
     7741 attgtcggcg tggcggatga cgctttgcgg cgttcgtcca acaaaatcgg ctcgattctg
     7801 attgcgggga tgacggttgc cggaagctga acgccctgcc gatgccgtct gaagacgaag
     7861 gcagcacgtt ttgccccgtg cctacccgtt tgccgtttta tgccgcaccg ccttttgccg
     7921 acaacattgc acattttttc atcagcactg attttccctt atccgtcaaa atactaagcc
     7981 acttgtcctc aacagggtac acacaagttc aggataaggc gtgcggggat tggggttttt
     8041 ccgccgccgg caaaacggca gacaaaaacg gcgggacatt gcctgtcccg ccgttttcaa
     8101 atatacattt gaccgtacat caggaattaa gcagcctgaa tgttggcggc ctgtttgcct
     8161 ttagggccgg tggttacgtc gaaagagacg cgttggcctt ctttcagggt tttgaaacct
     8221 tccatattga ttgctgagaa gtgagcgaac aaatcttcgc cgccttcatc aggcgtgatg
     8281 aaaccaaaac ctttagcgtc gttaaaccat tttacgatac cggttgccat tagaaacttc
     8341 ctatactcaa aaattaacaa aatcagcaaa acaaggcata cggcaaaccg agccgtctag
     8401 cgttccttcc agcttgatat actcggcgga tgcccaatcc tgcttaaccg tctttttaca
     8461 tttgttaaga caaaacgtca agctatgttt tcaaaaaagt gggaaaatag gcaaaagtac
     8521 cgaaaataca acaaaaatgc cgtctgaacg cggctttcaa atgggacaca ctatgaccgc
     8581 gcaacaccaa tccgacacgc tcttgcaccg tctgaacacc ctgccgccga aacgttacgg
     8641 cgttttccta ttgaacgacg attacaccac gatggaattt gtcgtcgaaa tcctgaccga
     8701 aatctttatg ctcggacaag aacaggcggt agcggtaatg ctcttggttc atcacgaagg
     8761 caaaggcctg tgcggcactt acacgcgcga tattgcccaa accaaacaac aacaagtcat
     8821 gcagcgggca aaagccgaag ggcatccgct gcaatgtatt gtcgaggaga tttaatatgc
     8881 ttgcacccga attggaacag attttgcagc agctttaccg cgaggcgcgt aaggctcatt
     8941 atgaatttat cagcctcgag catctgcttt tggtactcat cgaagaagat gcctccgtcc
     9001 acaacgtcct caagctctgc ggcgcggatt tgaaagtggt gtccgaacag ctcgccgcca
     9061 gcgttgccga aaacaccccc ctgattcccg aacacctttt agacacggtc gaaacccggc
     9121 ccacgctcgg cttccaacgc gtgatgcaac gggcgatggt gcatacccag tctgccggaa
     9181 aagccgcagt cgaaccgttg gacgttttgg tcgcgctgat gagcgaaacc gacagccaca
     9241 ccgtctattt cctcaagctg caatcggtta cgcgttttga agttttgcgc tgtattgccc
     9301 acggctctcc cgatgaagat gaagatgaag atgaagatga agacgatggc aactattctt
     9361 cagacggcat ggacgacgat aatggaaacc gcaccaaacc gggcaaaaac cctttatcgg
     9421 cgtacaccgt caacctcaac gccgaagtca aagccggccg tatcgaccct ttgattggtc
     9481 gcaaacacga aatggaacgg ctggtgcaaa tcctatgccg ccgccgcaaa aacaatccgc
     9541 ttttggtcgg cgaagcgggc gtgggcaaaa ccgcgctggc ggaaggtttg gcacatcaaa
     9601 tcgtcaacgg cggcattcca gacgcgctca aagatgccga agtgtacgcg ctggatatgg
     9661 gctcgctgtt ggcgggcacg aaataccgcg gcgactttga agcgcgggtc aaatccgtct
     9721 tgaaacagct cgaaaaaatc ccgcacgcca ttttgtttat cgacgaaatc cacaccatca
     9781 tcggcgcagg cagcaccagc ggcggcacca tggacgcgtc caacctgctc aaacccgcgc
     9841 tggcaaaagg ttcgctgcgc tgcatcggcg cgaccactta cgacgaatac cgcaccattt
     9901 tcgacaaaga ccacgcctta agccgccgct tccaaaaaat cgacgtggtc gaacccaccg
     9961 tttccgaaac cgtacaaatc ctgcgcggct tgaagccgat gtttgaagcc ttccaccaag
    10021 tccgctatac gcaaggcgca ctcgaagccg ccgccgaact ctccgcacgc tacatcaacg
    10081 agcgtttcct gcccgacaaa gccatcgacg tgatggacga agcaggcgcg gcgcaacgga
    10141 ttctgcccaa atccaaacag aaaaaagtca tcggcaaagc acaaattgaa accgtcatcg
    10201 ccaaagttgc gcggattccc gaaaaaaccg tgtcgcacga cgacaaacaa gtgctgcaat
    10261 tcctcggtcg cgatttgaaa aacatggttt acggtcaaga aaacgccatc gacacgctgg
    10321 ttgccgccgt caaaatgtcg cgttccggcc ttgccctgcc cgacaaaccg ataggcagtt
    10381 tcctcttctc cggtccgacc ggcgtgggca aaaccgaagc cgccaaacag cttgcctact
    10441 cgatgggcgt accgctgcaa cgctttgata tgtccgaata catggaacgc catgccgtat
    10501 cacgactcat cggcgcgcct ccgggctacg tcggctttga acaaggcggc cttttgaccg
    10561 aagccgtcaa caaacagccg cattgcgtat tgctcttgga cgaaatcgaa aaagcccacc
    10621 ccgacatttt caacgtcctc ctgcaagtca tggacgcagg caaactgacc gacaacaacg
    10681 gcaagagtgc cgatttccgc aacgtcatcc tgattatgac caccaatgca ggtgcggaga
    10741 gtctcagccg ccccagcctc ggctttaccg ccaagcgcga acgcggcgac gaaatgcagg
    10801 caatcaacaa gctcttcacg cccgagttcc gcaaccgctt ggacgcgatt atcccgtttg
    10861 cacccttgtc cgaacccatc atcgtcaaag tcgtggacaa attcctgctc cagctcgaac
    10921 accagctcct cgacaaaaaa gtcgaagccg aattcacgcc ggcattgcac aaatatctgg
    10981 cggaaaaagg ttttgacccg caaatgggcg cgcgcccgat gcaccgcctg attcaggaaa
    11041 aaatccgcaa accgctcgcc gacgaactgc tgttcggcaa actatccgac ggcggcttcg
    11101 tgcggataga ctgggatgcg gcaaaagaag aagccgtgtt gaaatttaag aaaagtaagg
    11161 tcaa
//
LOCUS       DAXHRI010000052        11068 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXHRI010000052 DAXHRI010000000
VERSION     DAXHRI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.052"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            204..1637
                     /gene="lpdA"
                     /locus_tag="ACFM75_001028"
     CDS             204..1637
                     /gene="lpdA"
                     /locus_tag="ACFM75_001028"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6255592.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLNIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGGCRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVIEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1762..2049
                     /locus_tag="ACFM75_001029"
     CDS             1762..2049
                     /locus_tag="ACFM75_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255593.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2103..3314
                     /gene="sucC"
                     /locus_tag="ACFM75_001030"
     CDS             2103..3314
                     /gene="sucC"
                     /locus_tag="ACFM75_001030"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC6255594.1"
                     /translation="MEANSGHSTNLKEKSMNLHEYQAKELLAGYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            3325..4215
                     /gene="sucD"
                     /locus_tag="ACFM75_001031"
     CDS             3325..4215
                     /gene="sucD"
                     /locus_tag="ACFM75_001031"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC6255595.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(4450..4779)
                     /locus_tag="ACFM75_001032"
                     /pseudo
     CDS             complement(4450..4779)
                     /locus_tag="ACFM75_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5133..7025)
                     /locus_tag="ACFM75_001033"
     CDS             complement(5133..7025)
                     /locus_tag="ACFM75_001033"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6255596.1"
                     /translation="MRIETENALELFFPKPALSLVYFEAIANSLDANATKINIEINIQ
                     DKTAFHTLKIKITDNGDGFIDENFNRFKTLLTPKDEAHKGIGRLVFLKYFKKVKIQSE
                     WLEGSKSFTFDKDDIKNITEGNKQNKTKKTELLFCEFTGKKIHKYDYIRPIELKFAII
                     EHFLPKLNTLTQQSIPFEITIDLKIDTDGGNLYSDKVSITKEDLPNLIIDTANPISIQ
                     SNLGQEYKIKTSYLIQEVDKISQLKVAFNIDNRTVLADNFLKRNVLPNTGYYCIFLFE
                     SELFNNCADMSRQRFNLPDEISENQLNRLLRNKIGEILSDRIPVISEKNKETKQNFER
                     DYPHLLGYFDKNIVGLIDKDEALDSAQSHLFQEQKRILEAENLSDEQYQKSLEFSSRV
                     LTEYVLYREKIISKMEDLVNSKSKEAEIHNLIIPRYHQFEQESLVSNIYQNNAWLLDD
                     KFMSFRTILSEQEMRKIIQEIQLDDEKKTSDERGRPDIALIFSADPTNNEKVDVVVIE
                     IKDSNDDLKNSMYAINQLLDRSNKLVNYCKNIQRMWYYAVVDIDHTMNRQLMQSGYSP
                     LFSKGKIFYKENKTFIYKDETESETIGEVPTPTFVISFDAIVNDAKSRNHTFLEVLRE
                     NMKRFA"
     gene            complement(7043..9901)
                     /gene="uvrA"
                     /locus_tag="ACFM75_001034"
     CDS             complement(7043..9901)
                     /gene="uvrA"
                     /locus_tag="ACFM75_001034"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC6255597.1"
                     /translation="MCKHRPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVITGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLIL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCESCQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDSGGRIIAEGSPEEVASMAKNSYTARYLKDILNKN"
     gene            10056..10853
                     /locus_tag="ACFM75_001035"
     CDS             10056..10853
                     /locus_tag="ACFM75_001035"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC6255598.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPVREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVKTVLARLPLTAP
                     QIESEPESEPALQTASVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
BASE COUNT         2810 a         2510 c         2784 g         2964 t
ORIGIN      
        1 gatgtagcgt aatgtagtat cgtgctacaa taggctcaac aaacgattga ggccgtctga
       61 aacatttgat tcgaatgaat cgacagatat agactttcag acggcctttt cttaaaacca
      121 tcaaaacgca gtcattcaaa ataaaaaaga aacgaaaagt atcgttttta ttttgagata
      181 ctgcaaaagc aaaggatgac acgatgtctc aatatgatgt agtagtgatt ggtgcaggcc
      241 cgggcggata cgttgccgcc atccgtgccg cacaactggg tttcaaaact gcctgtgtcg
      301 atgcaggcgt taacaaagca ggcaatgccc ctgcattggg cggtacttgc ttgaacgtag
      361 gttgtatccc ttctaaagcc ctgttgcaat ccagcgaaca tttccacgct gcgcaacacg
      421 attttgccga acacggtatc actgtcggtg acgtaaaatt cgacgcggcc aaaatgattg
      481 agcgcaaaga tgccatcgtg accaagctga ccggcggcgt caaattcctg ttccaaaaaa
      541 ataaagtaac cagcctgttc ggtacggctt cctttgctgg taaaaatggc gatgcttacc
      601 aaatcgaagt cgataacaaa ggagagaaaa ccgttatcga agccaaacac gtcatcgtag
      661 ccaccggttc cgtaccgcgt ccgctgccac aagtcgctat cgacaatgtg aacgtattgg
      721 acaacgaagg cgcattgaac ctgaccgaag tacctgccaa actcggcgtg attggttccg
      781 gtgtgattgg tttggaaatg ggttccgtat ggaaccgcgt gggtgcagaa gttaccattc
      841 ttgaagctgc gccgaccttc ctggctgccg ctgaccaaca aatcgccaaa gaagccttca
      901 aatacttcac caaagagcaa ggcttgaaca tcgaattggg cgtgaaaatc ggcgacatca
      961 aatctgaagg caaaggcgtt tccgttgctt acgaaactgc tgccggcgaa gccaaaactg
     1021 aagtattcga caaactgatc gttgccatcg gtcgtattcc aaacaccaaa ggcctgaacg
     1081 ccgaagccgt aggcttggaa aaagacgagc gcggctttat caaagtggac ggcggatgcc
     1141 gtaccaacct gcctaacgta tgggcaatcg gcgacgtggt tcgcggcccg atgttggcac
     1201 acaaagccag cgatgaaggc gttgccgttg ccgagcgcat tgccggtcaa aaaccgcata
     1261 tcgacttcaa caacgtaccg tttgtgattt acaccgatcc tgaaatcgct tgggtgggta
     1321 aaaccgaaga gcagctcaaa gccgaaggcg tggagtacaa aaaaggtact tcaggttttg
     1381 gtgcgaatgg tcgcgcattg gcaatgggca aagccaaagg tacggttaaa gtgttggcag
     1441 acgccaaaac cgaccgcatc ttgggcgtac acatgattgg tccggttgtc agcgaattgg
     1501 ttatcgaagg cgtgactgcg ctcgaattct tcgccagcag cgaagacatc gcccgcatta
     1561 tccatgccca cccaaccttg tccgaagtgg ttcacgaagc tgcattggcg gccgacaaac
     1621 gcgctttgca cggttgatag acattaaagt cgtctgaaat ttttcagacg accttaatgc
     1681 cttcgacaaa ttgaatgttc cgagagctcc gttttctgat ttataattcc gtcagacaaa
     1741 caaacagcat ttacattcgt tatgaacaaa gaaatagtcg gtattttctt tataccgatg
     1801 ggcatcatca gcatgtgtat ggccgcattg tggcagatgt atgtgatgat gaccgaaact
     1861 tatacgctca accgtttcaa agataaagaa ttggtttggc gcgtggcatt gttgtttatc
     1921 agtttcagcc ttgccgttta tctgctctgt ccgaattcgc gtaaaaaagg catcgtcttt
     1981 tttattctcg ggggaggcgg tgcagccatg tatctgctgg cgcggatgtg gttgcctttc
     2041 agcaagtgaa acgacgattt tccgaccgcc gaaaggtagt ctgaaacgca cgggcttgcc
     2101 atttggaggc aaactcgggg cattccacta atctaaagga gaaatccatg aatttacacg
     2161 agtatcaggc taaagaactg ctggctggct acggtttgcc cgtacaaggc ggtattttgg
     2221 cacacaacgg cgaagaagcc gctgcagctt acgacaaatt gggcggcaaa ttcgctgttg
     2281 tcaaagcaca agtacacgcc ggcggccgcg gtaaagcggg cggcgtaaaa gtcgttaaaa
     2341 gccgcgaaga agctaaagaa gtggctgaaa gcctgattgg caccaacttg gtaacttacc
     2401 aaaccgatgc caacggtcaa cctgtcaaca gcgttttggt ttgcgaagat atgtatccgg
     2461 ttcaaaccga gctgtacttg ggcgcagtgg ttgaccgttc tacccgccgc gttacattca
     2521 tggcttctac cgaaggcggt gtggaaatcg aaaaagttgc tgccgaaact ccagaaaaaa
     2581 tcttcaaagt aaccgttgat ccgctggtcg gcctgcaacc ttgccaagct cgcgaagttg
     2641 ccttccaact gggcttgaaa gacaaacaaa tcaacgagtt cgtcaaactg atgaccggtg
     2701 cgtacaaagc gtttgtcgaa aatgacttcg ccctgtttga agtcaacccg ctggcagttc
     2761 gcgaaaacgg cgcactggct tgcgtggacg gcaaaatcgg catcgacagc aacgcgctct
     2821 accgcctgcc gaaaatcgct gaattgcgcg acaaatctca agaaaacgaa cgtgaactga
     2881 aagcttctga attcgacctg aactatgttg ccctggaagg caacatcggc tgtatggtta
     2941 acggtgccgg tttggcgatg gccaccatgg acatcatcaa actgaaaggc ggccaacctg
     3001 ccaacttctt ggacgttggc ggcggcgcaa ccaaagaccg cgtggttgaa gcgttcaaac
     3061 tgattctgga agacaaatcc gttcaaggcg tattgatcaa catcttcggc ggtatcgtac
     3121 gttgcgacat gattgcggaa gccatcgtgg cagccgttaa agaaatcaac gtcgacgttc
     3181 ctgtcgttgt tcgtttggaa ggcaacaacg ccgaactcgg cgcgaaaatc ctgaacgaat
     3241 caggtctgaa actgacttct gcagacggcc tgaatgacgc agccgaaaaa attgttgcag
     3301 ccgtaaacgc ctaaggagaa aagaatgagc gtattgatta ataaagacac caaagtattg
     3361 gttcaaggtt tcaccggtaa aaacggtact ttccactccg aacaagctct ggcttacggc
     3421 actaaagttg tcggcggcgt gaccccgggc aaaggcggtc aaacccacct gaacctgccc
     3481 gtgttcaaca ccatgaaaga agccgttaaa gaaaccggcg ctgacgcatc tgtaatttac
     3541 gttcccgctc cgtttgtgtt ggattctatc gttgaagccg ttgattcagg cgtaggcttg
     3601 gtcgttgtga ttaccgaagg cgtgcctact ctggacatgc tcaaagccaa acgttatttg
     3661 gaaaccaacg gcaacggtac ccgcttggtc ggccctaact gccctggcgt gattactccg
     3721 ggtgagtgca aaatcggtat tatgccgggc cacatccaca ctccgggccg catcggtatt
     3781 atttcccgtt ccggtacatt gacttacgaa gctgtggcac aaaccaccaa actgggcttg
     3841 ggtcaatcaa cctgtatcgg tatcggcggc gacccgattc ctggtatgaa ccaaatcgac
     3901 gcactgaaac tcttccaaga agacccagat actgatgcca tcatcatgat cggtgaaatc
     3961 ggtggtactg cggaagaaga agcagccgaa tacatccaat ccaacgttac caaacctgtt
     4021 gtaggttaca ttgctggtgt taccgctcct aaaggcaaac gtatgggcca cgccggtgcg
     4081 attatctccg gcggtaaagg tactgccgag gaaaaattcg ccgctttcga aaaagccggc
     4141 atcgcctaca cccgcagccc tgccgagttg ggcactacca tgctggaagt gttgaaagca
     4201 aaaggtttgg cataatcagg tttgacaact gattaaacat caaatgccgt ctgaaaccgg
     4261 aaatcgggtt tcagacggca ttttgtttgt catttcaaaa agaggcagcc ttaacatacc
     4321 cacattattt tttgcccttt gaggcagtca gaatatttat cggtcaaaca ggagcaaacc
     4381 cgtaagtcag gttgcacata tttttttgaa gtcagggaat gataagtatc gcatttgaaa
     4441 agactgatat tattttttgc aggattctgt tttagcacgc gacatttttt gtttgatagg
     4501 attggaatgg atgtagtccg cacgatgctg caaatccgtt tcatcgcgca cggtgtgtcc
     4561 gtaaaaacgc cgttgccaaa taccgcgttc gtgccgctgt tgtttgccgg cggataaatt
     4621 tttagtatga ggggaatatg cggagaattt ggttttaatc agctatcgga gcagggaata
     4681 atccgcatca tcgggcggca gcgtccaaat ggcgtggata tggttcggca gcacgcatac
     4741 agcgacggtt tcaaagggat attgtttttg cgcatccata taagccgcac gcgaagccga
     4801 tacgttcgac aagcaggtgt gattttggat cggcgagttt gacggtgaaa aagaatgtgc
     4861 cgccggcaat gaagtttctg cgataacgcg ccataggatt tttgtttgtg gttgggggag
     4921 gggagaagga atggtttgtt gtttaaggtt ttaactttgt taaaagttga aacggcaaac
     4981 cgttaatcaa gcagcaaccg cgtgcgtacc gcatataccc tacacgttgg ttttgaagtt
     5041 ccgcataatt cacaggtagg gtgtgtggcg cagccacgca cacggtcggt tggatatgca
     5101 ggctacagct tattacaggt tatatacatt aattaggcaa atcttttcat attctcccgt
     5161 aaaacttcta aaaatgtatg attcctcgat tttgcgtcat taacaatcgc atcgaatgaa
     5221 atgacaaaag ttggtgtagg tacctctcct attgtttctg attctgtttc atctttatag
     5281 ataaaggttt tgttttcctt ataaaaaatc tttccttttg agaataatgg agaatacccc
     5341 gactgcatta gttgccgatt catagtgtgg tcaatatcaa cgaccgcata ataccacata
     5401 cgttgaatgt tcttacaata gtttactaat ttattagagc gatctagtaa ttgattaata
     5461 gcatacatac tattttttaa atcatcattt gaatctttaa tctcaattac tactacatca
     5521 actttttcat tattagtagg atcagcagaa aaaattaaag caatatcagg tcttcctctc
     5581 tcatcagatg tcttcttctc atcatccaac tgaatttctt ggataatttt tctcatttcc
     5641 tgttcgctta aaatggttct aaatgacata aatttatcat ccaataacca tgcattattt
     5701 tgataaatat tagaaacaag actctcttgc tcaaattgat ggtatcttgg aataattaaa
     5761 ttatgtatct ctgcttcttt gctcttgcta ttaactagat cctccatttt agaaataatc
     5821 ttttcacgat aaagtacata ttcagttaat actctcgatg aaaattcaag agatttttgg
     5881 tattgttcat cggaaagatt ctctgcttct aaaattcttt tttgctcctg aaaaagatga
     5941 gattgagcgc tatctaatgc ttcatcttta tcaattaaac caacaatgtt cttatcaaaa
     6001 tacccaagca aatgagggta atccctctca aaattttgct tagtctcttt attcttctct
     6061 gaaataactg gaattctatc agataaaatt tctccgattt tattacgtaa taatctgttt
     6121 aattggtttt cacttatttc atcaggtaaa ttgaatcttt gacgagacat atctgcacag
     6181 ttattaaaca actctgattc aaataagaat atacaataat agcctgtatt aggtaaaaca
     6241 tttcttttta agaagttatc agccaagact gtacggttat caatattaaa agccactttc
     6301 aattgactaa ttttatcaac ctcttgaatt aaataagaag ttttgatttt atattcttgt
     6361 cctaaattgc tttgtatgga tataggatta gctgtatcta ttattaaatt tggcaaatct
     6421 tccttagtga tagatacctt gtcgctatat aaattaccac catccgtatc tatttttaaa
     6481 tctatcgtta tttcaaacgg aattgattgt tgagtaagtg tgtttagctt tggtaaaaaa
     6541 tgctcaataa tagcaaactt aagttcaatt ggtcgtatat aatcatattt atgtattttc
     6601 ttcccagtaa attcacagaa taacaactct gttttctttg ttttattttg tttatttcct
     6661 tctgtaatgt ttttaatatc atctttatca aaagtaaatg atttactacc ttccaaccat
     6721 tcgctttgaa tttttacttt cttgaaatat ttcaaaaaaa ctaaacgtcc aataccttta
     6781 tgtgcttcat cttttggagt taataatgtt ttaaatctat taaaattctc atctataaat
     6841 ccatctccat tatcagtaat cttaattttc aaagtatgaa aagccgtttt atcttggata
     6901 ttaatctcaa tattaatttt tgtagcattt gcatctaatg aattagcaat agcctcgaaa
     6961 taaactaatg ataatgcagg ttttggaaaa aagagttcta atgcattttc tgtttcaatt
     7021 ctcatggaaa tatcctcaga tgttaattct tatttaagat atctttcaaa tatcgagcag
     7081 tatagctatt tttggccatg ctagccactt cctctgggct accttccgca ataatcctcc
     7141 ccccgctatc gccgccttcc ggccccaaat ccacaatcca atccgcagtt ttaatcacgt
     7201 caagattatg ctcgataatc acaatcgagt tgcctttgcc tttcagacgg cctatgactt
     7261 ccagcagcag ggcgatgtcg gcgaagtgca ggccggtggt gggttcgtcg aggatgtaga
     7321 gtgttctgcc ggtgtcgcgt ttggatagtt ccaaggcgag tttgacgcgt tgggcttcgc
     7381 cgccggagag ggtggtggcg gattggccga ggcggatgta gccgaggcct acgtccatca
     7441 gagtttgcag tttgcgcgat acggtgggga cggcgtcgaa aaattcgcgg gcttcttcga
     7501 cggtcatgtc gaggacttgg ctgatgtttt tgcctttgta ttggatttcg agggtttcgc
     7561 ggttgtagcg tttgccgtgg cagacttcgc aggggacgta cacgtcgggc aggaagtgca
     7621 tttcgacttt gatgacgccg tcgccttggc aggattcgca gcggccgcct ttgacgttga
     7681 aggagaatct gccgacgttg tagccgcgtt cgcgcgagag gggtacgccg gcgaagagtt
     7741 cgcggatggg ggtgaacagg ccggtgtagg tggcggggtt ggaacgcggg gtgcgaccga
     7801 tgggggattg gtcgacgttg atgactttgt cgaggtgttc gaggccgcgg atgtcgtcgt
     7861 atggggcagg ttcttcttgg gcgcggttga gttcgcgggc ggtgattttg gcgagggtgt
     7921 cgttaatcag ggtggacttg ccgctgccgg atacgccggt gatgcaggta atcaaaccga
     7981 gcggtaattc gagggtgacg tttttgaggt tgttgccgcg cgcgcctttg aggatgagca
     8041 ttcggtcggg attgacgggc gtgcgttcag acggcacggc aatggatttt ttgccgctga
     8101 ggtattgtcc ggtaacggag ttttcgcatt tggcgacgtt ttcgggggta tcggcaatca
     8161 gtacgttgcc gccgtgttcg cccgcgccgg ggcccatatc gacgacgaaa tcggcttcgc
     8221 ggatggcgtc ttcgtcgtgt tcgaccacaa tcacgctgtt gcccaagtcg cgcaggcgtt
     8281 tgagggtggc gagcaggcgg tcgttgtcgc gctggtgcag gccgatggag ggttcgtcca
     8341 aaacgtacat tacgccggtc aggccgctgc cgatttggct ggcgaggcgg atgcgctggg
     8401 cttcgccgcc ggaaagggtt tcggcggagc ggcttaaatt caggtaatcc agcccgacgt
     8461 taatcaggaa gccgagccgc tcggtgattt ctttgaggat tttttcggcg atttgttttt
     8521 tgttgccgtc caaatccagc gtttcaaaga attggtgggt tttggtcagc ggccaggcgg
     8581 agacttcgtg caacggttcg ccgctgacgt aaacgtagcg cgcttctttg cgcaaacgtg
     8641 caccgccgca gctcgggcag gcgcggtggt tctggtattc gcgcaatttt tcgcgcacgg
     8701 tttcgctgtc ggtttcgcgg tagcggcgtt cgagattggg aatgatgcct tcgaaggcgt
     8761 ggctgcggtt gaaggtggtg ccgcgttcgg ataggtaggt gaaatcaatg acttccttgc
     8821 ccgagccgtg caacacgact tttttgattt ttgcaggtag ggtttcccaa gcagcctgca
     8881 catcgaaacc gtaatgccgc gccagcgact gaatcatttg gaaatagaac tggttgcgct
     8941 tgtcccagcc gtcaatcgcg cccgaagcca acgacaattc gggatgcgcg accacttttt
     9001 cggggtcgaa gaaattggtg ttgcccaagc cgtcacaagt cggacaggaa cccatcgggt
     9061 tgttgaacga aaagaggcgc ggctccaatt cgggcaggct gtatgaacat acgggacagg
     9121 caaaacgcgc ggaaaaccaa tgttcttcgc cgctgtccat ctccatcgcc agcgcgcgct
     9181 cgttgccgtg gcgcagtgcg gtttcaaaac tttccgccag ccgctgcttg atgtccgcct
     9241 tcactttcac gcggtcgatg accacgtcga tattgtgttt gatgtttttc tccagcttcg
     9301 gcacttcgtc aagctgataa acttcgccgt ccacgcgcac acgggcaaag ccttgcgcct
     9361 gcaagtcagc aaagaagtcg acaaactcgc ccttacgctc acgaactgcc ggcgcaagaa
     9421 tcatcacgcg cgtgtcttcc ggcagtttta atacggcatc gaccatctga gatacggttt
     9481 ggctggatag cggcagattg tgttcggggc aatacggcgt gccgacgcgt gcgtacaaaa
     9541 ggcgcaggta atcgtggatt tccgtaaccg tgccgacggt ggaacgcgga ttgtggctgg
     9601 tggatttctg ctcgatggaa atcgcaggcg acagaccttc gatcaaatcg acgtcgggtt
     9661 tgtccatcat ctgcaaaaac tgccgcgcat aggcggaaag gctctcgaca taacgccgct
     9721 gcccttcggc atacagcgtg tcaaacgcca gcgacgactt gccgctgccc gacaatcctg
     9781 ttatgaccac gagcttgtgg cgcggaatgt ccaaatcgat gtttttcaaa ttatgcgtgc
     9841 gcgcgccgcg tatgcggatg gtgtcgttgt cgtgcgaatg ttggggacga tgcttgcaca
     9901 taatggatgc cgcctgaaaa ataaaggaaa accgatattg tagcactttc tcggatgccg
     9961 tctgaagccg cgttcagacg gcatttgtcg gcggaacgcg gcggattccg ttataatggc
    10021 ggcaatttta acccgcttga acaaaaggat gacaaatgaa ccgtctttac ccccacccga
    10081 ttatcgcccg tgagggctgg ccgattattg gcggcggttt ggctttgagt ctgctggtgt
    10141 cgatatgttg cggctggtgg tctttgccgt tttgggtgtt taccgtattt gcattgcagt
    10201 ttttccgcga ccctgtgcgt gaaattccgc aaaatcctga agcggtgttg agccctgttg
    10261 acggccgcat tgtggtggtc gaacgcgcac gcgatccgta tcgtgatgtc gatgctttga
    10321 aaatcagtat ttttatgaac gtgttcaacg tgcattcgca aaaatcgcct gccgattgta
    10381 cggtaacgaa agtggtctat aacaaaggca aattcgtgaa tgcggatttg gacaaagcca
    10441 gcacggaaaa cgaacgtaat gcggtgttgg cgactacggc ttccggtcgt gaaattactt
    10501 ttgttcaagt ggccggtttg gtggcgcgcc gtattttgtg ctacacccaa gcaggtgcga
    10561 aactgtcccg cggcgaacgt tatggcttta tccgcttcgg ttcgcgcgtg gatatgtatc
    10621 tgcctgtcga tgcgcaggcg caagtggcga ttggcgataa agtaaccggc gtaaaaactg
    10681 tattggcgcg tctgccgctg actgcgccgc aaatcgaatc cgagcctgaa tctgagccag
    10741 ctttacaaac tgcttcggtt gaaacagcgg caaacccatc tgccgaacaa cggcaaattg
    10801 aagcagcggc ggctaagatt caggcggctg tgcaagatgt gttgaaagat taattttgcg
    10861 aactgaaata gaaaatatca gtaccatcat tcacacgaat gaggaagttt ggttttttga
    10921 atttttgcta atgttcacac cgttatgttc acgaaaatgg gaatctggaa atttaacgtt
    10981 acgacgattt atcggaaacg actgaaaccg gacggactgg attcccgcct gcgcgggaat
    11041 gacgactcat tagttaccta aaacttaa
//
LOCUS       DAXHRI010000053        10721 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXHRI010000053 DAXHRI010000000
VERSION     DAXHRI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.053"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(13..1215)
                     /gene="trpB"
                     /locus_tag="ACFM75_001036"
     CDS             complement(13..1215)
                     /gene="trpB"
                     /locus_tag="ACFM75_001036"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC6255599.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1298..1987)
                     /locus_tag="ACFM75_001037"
     CDS             complement(1298..1987)
                     /locus_tag="ACFM75_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6255600.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDPVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKTFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            2199..2978
                     /gene="rsmA"
                     /locus_tag="ACFM75_001038"
     CDS             2199..2978
                     /gene="rsmA"
                     /locus_tag="ACFM75_001038"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC6255601.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELATDEDLQAAGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            3151..3879
                     /locus_tag="ACFM75_001039"
     CDS             3151..3879
                     /locus_tag="ACFM75_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6255602.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQGFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            3952..4311
                     /locus_tag="ACFM75_001040"
     CDS             3952..4311
                     /locus_tag="ACFM75_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255603.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            4316..4756
                     /locus_tag="ACFM75_001041"
     CDS             4316..4756
                     /locus_tag="ACFM75_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC6255604.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKESYRRLKVWILWRQPKKTAETDTPS"
     gene            4788..6062
                     /gene="folC"
                     /locus_tag="ACFM75_001042"
     CDS             4788..6062
                     /gene="folC"
                     /locus_tag="ACFM75_001042"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC6255605.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPKSLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDMPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6075..7073
                     /gene="ftsN"
                     /locus_tag="ACFM75_001043"
     CDS             6075..7073
                     /gene="ftsN"
                     /locus_tag="ACFM75_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6255606.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINERLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7066..7563
                     /locus_tag="ACFM75_001044"
     CDS             7066..7563
                     /locus_tag="ACFM75_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC6255607.1"
                     /translation="MNSLPIADLLVSAVIAACIVISTMRGVITEAGSMAAWVVSFFFA
                     KLFAAPFADIAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            7599..7730
                     /locus_tag="ACFM75_001045"
     CDS             7599..7730
                     /locus_tag="ACFM75_001045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255608.1"
                     /translation="MPSEMFFPFLSHITDYSGLNLNQYGVASPCRTICTVCGFVALS"
     gene            7875..9419
                     /gene="purF"
                     /locus_tag="ACFM75_001046"
     CDS             7875..9419
                     /gene="purF"
                     /locus_tag="ACFM75_001046"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC6255609.1"
                     /translation="MCGVLGLVSHEPVNQFLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            9519..10010
                     /gene="greB"
                     /locus_tag="ACFM75_001047"
     CDS             9519..10010
                     /gene="greB"
                     /locus_tag="ACFM75_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC6255610.1"
                     /translation="MSTETKNYITPTGWQALKDELYRLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            10069..10695
                     /locus_tag="ACFM75_001048"
     CDS             10069..10695
                     /locus_tag="ACFM75_001048"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC6255611.1"
                     /translation="MRKIRTKICGITTPEDALAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVVLFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIQNAAHRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAESVDVSGGVEASKGKKDPAKVAAFISTANRLSR"
BASE COUNT         2677 a         3090 c         2758 g         2196 t
ORIGIN      
        1 agacggacga ggttacagtt taatcccttt gagcttcgcg acggtattga tgtctttgtc
       61 gccacgacct gagaggttga ccaaaatcac ttggtcttta cccattttcg gcgcattttt
      121 caccgcccaa gcaacggcgt ggctggattc gagcgcgggg atgatgccct cgaagcggca
      181 gagcaagtca aaggcttcga gtgcttcgtc gtctttggca acggtgtatt cgacgcgctt
      241 gatgtcgtgc agatggctgt gttccgggcc gatgccgggg taatccaagc ctgcggaaac
      301 agagtgcgta cccaaaacct gaccgttttc gtcctgcatc agatagctgc ggaaaccgtg
      361 caatacgcca atcggtgcgc ccgaagtaat cggcgcggcg tgatcggggg tgttcacgcc
      421 caaaccgcca gcctccacgc cgacgaggcg cacgttttct tcgccgatat acgggtggaa
      481 caaaccgatg gcgttcgatc cgccgcccac gcaggcaacg gcaacgtcgg gctgtctgcc
      541 gatggcttcc aacatttgtt ctttggcttc attgccgatc acgcattgga aatcgcgcac
      601 catttcggga tacggcgcgg ggccggcggc ggtaccgatg atgtagaatg tgtcatccac
      661 gcgggcgacc cattcgcgca tggcttcgtt catcgcgtct ttcaaagtac ggctgccgct
      721 gtcaacgccg acgacattcg cgcccaataa tttcatacgg aacacgttgg gcatttggcg
      781 ttggatgtcg tccgcgccca tatacacgtc gcaagtcata ccgaagcgtg cggcaacggt
      841 ggcactcgcc acgccgtgct gacccgcgcc ggtttcggcg atgacgcgtt ttttacccat
      901 gcgttttgcc aacagtgcct gaccgatggt gttgttgact ttgtgcgcgc cggtgtggtt
      961 caagtcttcg cgcttcaacc agatttgcgc gccgcccaga tgttcggaca accgcgcggc
     1021 gtggtaaacg gggctgggcc tgccgacata atgtttcaaa tcatggcgga acgcttccca
     1081 aaattcagga tcgtttttcg ctgccttata ggcatccgcc agctcttgca aggcgggaat
     1141 cagggtttcg gagacataaa gcccgccgtg ttcgccgaaa aagcccttct cgtcgggcgc
     1201 gtggtagttc ttcatcggat tcttccttgt gcttcaaatt ttcagacggc atttccgttt
     1261 ttgcaaacat gccgtctgaa cgggaaattg tcggcaatta taactgtttt tcacacaagc
     1321 ccgccatttc gaaaaacgcc cgatacgccg ccgctttttt cgccaaactc aaaggatagg
     1381 cgcgcgaagt cgaaggcagg cgcgtcagcg tcaaatcccg tccggcaaac ggaaacggca
     1441 cggtttcgcc cgttttcggc attttgatac cgccgccctg aatatcgagc aggatttccg
     1501 tcgccttgcc gcctgtcgta caaatatggc ggcactcggg tatttttgcc aacaccgccg
     1561 ccaaatcgac ggtttcaact acctttaaaa acttgtcgga cgcattgccg tgttcccttg
     1621 ccgccttcaa cacggtcgga caggacgcaa tcccccgttc gtgcaaaaac gttttgattt
     1681 tctccgcgtc aaacgctttt tcagacaacc tttggaaatg cgccgcatca ttaaaaaaca
     1741 ccagcccgta aacgcgccac atatcgtttt ggaaattcgg ataatgaaac tgcatcgcgc
     1801 gtttgtcttc cttgggcgga aacgtcccca tcatcatgac cgcagccttc ggcggcaaca
     1861 cgggatcgaa gggatgggtt tcgatttcaa gcagattttc agacacgttc aatcctccaa
     1921 caggcaaaac ggcagacagc cggtttttgc accaaaccgc caaacacggc aagccttcaa
     1981 acagcattat cacgccctgc cgttttcggt tacaatgccg acatcaaacg gatactgtaa
     2041 acacatttat gtttcaacac acaggacgac acataaagca ccgccctatg tgccgtcctg
     2101 atttggaagg agttacgccc ctctcaaata gagtctgatt ctaccgcccc gaaggacaga
     2161 tgtccaagtg gcggggtttc aaccgaaaag gaaacacgat gaaagaacac aaagcccgca
     2221 agcgtttcgg gcagaatttt ttgcaggaca cgcggatcat cagcgatatt gtcaacgccg
     2281 tgcgcccgca ggcggatgat gtcgtgattg aaatcggccc gggtttggcg gcgattaccg
     2341 aacctttggc gaaaaagctg aaccgcctgc acgttgtcga aatcgaccgc gacatcgtat
     2401 gccgtctgaa aacgctgccg tttgcggata aactggtgat tcacgaaggc gatgtattgc
     2461 agtttgattt caacggcatc gcaggcaaaa agaaaatcgt cggcaacctc ccgtacaaca
     2521 tttccacacc gcttttgttc aagctggcgg aggtggcgga cgatgtcgtc gatatgcact
     2581 ttatgctgca aaaagaagtg gtcgatcgta tggttgccgc gccgaaaagc aacgactacg
     2641 gccgcttggg cgtgatgctg caatattttt tcgatatgga gctgctgatt gacgtaccgc
     2701 ccgaatcgtt cgatcctgca ccgaaagtgg attccgccgt cgtccgtatg attccggtga
     2761 aacaccgcat cggcaaggcg gacgatttcg agcatttcgc caaactcgtg aaactcgcct
     2821 tccaccaacg ccgcaaaacc atacgcaaca acctgaaaga actggcaaca gacgaagatt
     2881 tgcaggcagc cggcatcaat ccgcaagacc gtgccgaaca tatcgcgccg gagaaatatg
     2941 tggcgttgag caattatctg gcgggcaagg ccgtctgaaa agggagtaag ccgatgacga
     3001 aatacctgct tttacccctg atactgccgc ttgcagcctg cctgaccaat ccgaacgacc
     3061 gtttttttga cggcagacgc tacacgcttc atcatatgga gctcccgtaa cgacaacctg
     3121 caacggcaaa tgccgtctga aagaaaagac atgatcaaat tcaaaaacgt acacaaacat
     3181 ttcaaagacc tgcacgtcat caacggcgtg aacttggaaa tcaaaaaagg cgaagtggtc
     3241 gtcgtctgcg gaccttcggg cagcggcaaa tccacactca tccgcaccgt caaccagctt
     3301 gaaagcatcg aaagcggcga gatttgggtg gacggtgtca acgttgccga ccccaaaacc
     3361 gatttgaaca aaatccgcga agaagtcggc ttcgtattcc aaggcttcaa cctctacccc
     3421 cacctgaccg tattggaaaa catcaccctc gcaccgatga aggttaaagg tcaaaatgcg
     3481 gaacaggcgg agaaaaaagc gatggaactt ttggaacgcg tcggactggc acacaaaaaa
     3541 gacgccttcc cttcccaact ttccggcggc cagcagcaac gcgtggcgat tgcacgcggt
     3601 ttggcgatgg aaccgcgcgt aatgttgttt gacgaaccga cctccgcact cgaccccgaa
     3661 atggtcggcg aagtgttgaa agtgatgaaa gacttggcag aaagcggcat gacgatgatg
     3721 tgcgtaaccc acgaaatggg gtttgcccgc gaagttgccg accgcgtgat tttcgtcgat
     3781 aaagggcaaa tcctcgaaga cgaaacgccg gaagcgtttt ttaccaaccc gaaacacgaa
     3841 cgcgccaaac aattcctgca acaggtcatg acccattgat gctatgccgt ctgaaaaccg
     3901 gacgttcaga cggcatagcc cgacaccgca agatgattga gaaagaaaac catgtatttt
     3961 gttgaccgca ccgccgtcgt cctcaagccg accgtccgct ttttggaatg gctcaaaagc
     4021 accgacgaaa atatgcccga cctgaccata gaacagcttc gtgccaactg ttccgtcttc
     4081 ctcgttccgc agttcgacga accggaagcc gtcgtttcct atttcgacga acgctaccgc
     4141 cagattttcg aggcggagct ggcaggttgg gacatcgata aggataaatg gccgcaagat
     4201 atggggctga aggcgttttg ggaatttttc gacatcgaaa tccacgatat ggtgttggac
     4261 atggaagaag cggaattgaa catcacgccc gtgttcgaca acatgatgta aggctgtgcg
     4321 cgcctttcag acggcattga ggccgtcccg catcctgaac atcctgactg tatcgcttca
     4381 cgccgcctcc cttgccgtct gcctgacttg gttttacggc tggatgatgt ggttcggttt
     4441 ggcggctttg gtggcaagtt acgcatactc gctcaggata acgaatctga aacaccgcca
     4501 tgccataacc gccattacca tagaccgcga cggacaggcg gaaatcgtat ccggcaaaga
     4561 caaaacagcg gcaacacttg cgggcagcag tatggttacg ccttatgcct tattcttaca
     4621 atgggacaca ggcggcacaa ccgtcagcca atgcatcata cccgacatga cggacaaaga
     4681 atcctaccgg cggctgaaag tctggatact ttggcggcag ccgaaaaaaa ccgccgaaac
     4741 cgatacacca agctgatttc agacggcatt tgaacacgga acactcaatg aaaacactgc
     4801 aagactggct ctcgcatttg gaaaccgccc acagcggcgg cttgatcgat atgggcttgg
     4861 aacgcgtagg cgaagtgaaa aaacggatga agctcacgcc gcaatgcccc gtcgtcgttg
     4921 tcgcgggaac aaacggcaaa ggttcggtct gcgcctacct gacacagatt tacaaacagg
     4981 cgggctacaa aaccggcaca ctgaccagcc cgcatttatt gcgttacaac gaacgcatcg
     5041 ccatcaacgc cgaacccgtt tcggacgata cgattattgc ctctttcgag cgcatcgaag
     5101 cggcgcgcag cgaaatatcg ctgacttatt ttgaattcaa tacgttggcg gccgtcgaca
     5161 tcttcatgcg cgaacaggtt gacgtgatga tattggaagt cggcttgggc ggacgcttgg
     5221 acgcggtcaa cgcgtttgac ggcgattgcg cggtggttac cagcgtggat ttggaccatc
     5281 aggcattttt gggcgatacg gtcgagcagg tcggctttga aaaagcaggc gtgttccgta
     5341 gcggcaaacc cgccatctgc ggtcaaaacc ccgcgcccaa atcgttgctt tcacacgccg
     5401 aagccatagg cgcgaaactg ctgatggtgc agcgcgattt cgagtttcac gcgctggaaa
     5461 acatccaatg gaactaccgc ttccgaccgc agcattcaga cagccccgct cgcaaccgta
     5521 attccctgcc cttccccgca ttgcgcggcg cataccaact ttccaatgcc gcctgtgcgc
     5581 tgaccgtgtt ggaatgtttg aacgacaaat tgccggtgga catcggtgca atcaaacgcg
     5641 gattgctgct ggttgaaaat cccggacgct tccaagtcct gcccggccgg ccgctgaccg
     5701 ttttggacgt cggacacaat ccccacgctg cccgcgccct gcgccgcagc ctgattaatt
     5761 tggcgtttgc gcaaaaacgc accgccgtgt tcagtatgct gtccgacaaa gacatagacg
     5821 gcgtgttgga aattgtcaaa gaccagtttg acgaatggta tatcgcacct ttggacatgc
     5881 cgcgcggcat gacgctcgag agcctgcaaa acaaactgca cgaacacagc atcgaaaaca
     5941 tacagacatt cgccgccgtc cgcgaagcat accgggcggc agcctcaaaa gcgggcgaga
     6001 acgacagaat cgtcgttttc ggctcatttc ataccgttgc cgatgtcatg tcggtacttt
     6061 aaaaggaaac ttctatgtcc gaaaacaaac aaaacgaagt cctgagcggt tacgaacaac
     6121 tcaaacggcg caaccgccgc cgcctcgtaa cggcaagttg cttggttgcc gcctcctgca
     6181 tcctgctggc agccgccctc agttccggcc ctgccgaaca gactgccggc gaaacaagcg
     6241 gcgtagaaaa caaagcggca ggtgcggcac aaacccctgc cttgaaatcc gccgccgaca
     6301 aaccgcagga cttggcaggc gaagacaagc cttctgccgc cgacagcgaa atcagcgagc
     6361 ctgaaaacgt aggcgcgccg ctggtgctga ttaacgagcg cctcgaagac agcaacatca
     6421 aaggtttgga agcatccgag aaactgcaac aggcagaaac cgccaaaacc gcaccgaagc
     6481 aggcaaaaca acgcgctgcc gaaaaagtgc cggcaactgc cgacagtacg gatacggtag
     6541 cggttgaaaa accgaaacgc actgccgaaa caaaaccgca aaaagcggaa cgcactgcca
     6601 aagccaagcc caaagccaaa gaaaccaaaa ccgccgaaaa agttgccgac aaaccgaaaa
     6661 ctgccgccga aaaaaccaaa ccggatacgg caaaatccga cagcgcggta aaagaagcga
     6721 aaaaagccga caaggctgaa agcaaaaaaa cagccgaaaa agaccgttcg gacggcaaaa
     6781 aacacgaaac ggcacaaaaa accgacaaag cggacaagac caaaaccgcc gagaaggaaa
     6841 aatccggtaa aaaagccgcc attcaggcag gttatgccga aaaagaacgc gccttaagcc
     6901 tccagcgcaa aatgaaggcg gcgggtatcg attcgaccat caccgaaatt atgaccgaca
     6961 acggcaaagt ttaccgcgtc aaatcaagca actataaaaa cgcaagggat gccgaacgcg
     7021 atttgaacaa attgcgcgta cacggtatcg ccggtcaggt aacgaatgaa tagcctcccc
     7081 attgccgacc tcctcgtctc cgccgtcatc gccgcctgca tcgtcatttc cacgatgcgc
     7141 ggcgtgatta cggaggcagg ctcaatggcg gcatgggtgg tttccttctt tttcgccaaa
     7201 ctctttgccg cacccttcgc cgacatcgcc tttgcatcgt tccaaccccg cctgtttgca
     7261 ttggctctgt cgttcatttc cctattcgtc attgcctgtc tgatccagaa aatactccgc
     7321 tcgctgctga ccggggcagt ttcggcggtc ggtttgggct ttgccaaccg cattttgggc
     7381 ggcgtattcg gtgcattgaa aggcattttg attattaccc tgctggtcat gctcgcttca
     7441 aaaaccgacc tgcccgatac cgaagaatgg cggcaatctt acacactgcc gtttttcgta
     7501 tcgctttccg aagccgtgtt gaaccatagc ggcggcacgg cggaaactcc ggaagacgat
     7561 tgattgccat acggatattt cagacggcat tctcttttat gccgtctgaa atgttttttc
     7621 ctttcctcag ccatatcaca gattatagtg gattaaattt aaaccagtac ggcgttgcct
     7681 cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
     7741 tccactataa atcttattct ccataaaccc acctatctgt ccttcgctca aatattgtca
     7801 acaattcagg caaaaaacca ccgtttactt tataattccg ttttaccctt aaacctcgaa
     7861 tcggagaaga cgatatgtgc ggcgtattag gtttggtcag tcatgaaccc gtgaaccagt
     7921 ttctgtacga cggcttgcag atgttgcagc acagggggca ggatgcggcg ggcattgcaa
     7981 cggcggaagg cggtaccttc catatgcaca aaggcaaagg catggtgcgc gaagtgttcc
     8041 gcacacgcaa tatgcgcgat ttgaccggca acgccggtat cgcccacgtc cgttatccta
     8101 ctgcgggcaa cgcaggcagc agcgcggagg cgcagccttt ctacgtcagc tcgccgttcg
     8161 gcatcgtttt ggcgcacaac ggcaacctca ccaacactgc cgaactgtat gaaaacgtgt
     8221 gtaacaaaca cctgcgccac gtcaacacca gctccgattc cgaagtcttg ctcaacgtat
     8281 tcgcgcacga attacgccgc gaagtctcta aaaacgccga cccgcaccgg ctcaatgccg
     8341 acaatatttt caacgccgtt gcccaagtcc accgcctggt gcgcggcgca tacggtgtga
     8401 ttgccatgat tgcgggctac ggtatgctcg ccttccgcga cccttacggc atccgcccgc
     8461 tggtattggg ttcgcaaacc gacagcgaag gcagaaaatc ctatgccgtc gcctccgaat
     8521 ccgtcgcctt caacgcgctt acctacgatt tggaacgcga catccggccg ggcgaagcgg
     8581 tatttgtcgg ctttgacggc acaatgattg cccgccaatg cagcgaccgt gccaaactca
     8641 gcccctgcct ttttgaatat gtttattttg cccgccccga ctctgtgatt gacggcgtat
     8701 ccgtttacca atcgcgcttg gatatgggcg tgtcgctggc ggaaaaaatc aaacgcgagc
     8761 tgcccgtgga cggcatcgac gttgtgatgc ccattcccga caccagccgc cccagcgcga
     8821 tggagcttgc cgtccatctc gacaaaccct accgcgaggg tttgattaaa aaccgctata
     8881 tcggccgcac ctttattatg cccggacagg cgacgcgcaa aaaatccgtg cgccaaaaac
     8941 tcagcccgat ggaaaccgaa tttgcaggca aaagcgtgtt gctggtggac gactccatcg
     9001 tgcgcgggac gaccagccgc gaaatcgtcg aaatggtacg cgcggcgggc gcgcgcaaag
     9061 tctatatcgc ctccgccgcg cccgaagtgc gctatcccaa tgtgtacggc attgatatgc
     9121 ccacgcgcga agagttgatt gccaacgggc gcagcgcggc ggaaatcgcc gccgaaatcg
     9181 gcgcggacgg catcgtattt caagacttgg gcgatttgga agccgtcgtc aaagcactca
     9241 atccgaaaat cgaatccttc gattcgtcct gtttcaacgg catttatcag accggcgaca
     9301 tcgatgatgc ctacctcgac cgcctgtccg ccgagaaatc cggctgcggc ggcctgaaaa
     9361 tccacccgag caggatggaa cacagcatca gcatcagcga tgcgggcgac gaagaataag
     9421 acttcccaaa ccgcgatgcc gtctgaaatc cgaacgccgg gttttcagac ggcatttccc
     9481 atatcggcat cacatttccc atttcatccg cacgcaatat gagtaccgaa accaaaaact
     9541 acatcacgcc gaccggctgg caggcattga aagacgaact ttaccggctg gtcaacaaag
     9601 aacgccccga aatcgtccaa atcgtcaact gggcggcagg caacggcgac cgcagcgaaa
     9661 acggcgacta tctttacggc aaacgccgta tgcgcgaaat cgaccgccgc atccgtttcc
     9721 tgaccaaacg tttggaggct gccgtcgtcg tcgatcccga attgcgcgaa gcgaccgacc
     9781 aagtgttttt cggcgcgacg gtcgggctgt tgcgcggcga cgggcgcgag caaaccgtca
     9841 aaattgtcgg catcgatgaa atcgataccg cgcaaaacaa aatttcttgg atttcgccgt
     9901 tggctcgttg cctgattaaa gcacgcgaag gcgacgaagt ggttttgaac acgccggaag
     9961 ggcgtgagga aattgaaatc ctttctgtcg aatacatcaa aatcgactga tcgggaatgc
    10021 cgtctgaaac ggtgcaagcg caccgttttc caaataaagg aataccgaat gaggaaaatc
    10081 cgcaccaaaa tctgcggcat caccacaccg gaagacgcat tggccgccgc agcggcaggt
    10141 gcagatgccg tcgggctggt ctttttccaa ggcagcagcc gggccgtcga tattgcccgc
    10201 gccaaaaaaa tcaccgccgc actgccgccg tttgtcagcg ttgtcgtcct tttcgtcaac
    10261 gaaagcgcgc aaaacatccg ccgcatcctt gccgaagtgc cgatacacat catccaattc
    10321 cacggcgacg aagacgacgc attctgccgc cagttccacc gcccctatat taaagccatt
    10381 cgtgttcaga cggcatcaga catccaaaac gccgcccacc gcttccccga cgctcaggca
    10441 ctgctgttcg atgcctatca cccttcggaa tacggcggca ccggacaccg cttcgactgg
    10501 acgctgttgg cggaatattc gggcaaaccg tgggtgcttg ccggcgggct gacccctgaa
    10561 aacgtcggcg aagccgtccg cattaccgga gcggaatcgg tcgacgtatc cggcggcgtg
    10621 gaagcgtcta aaggcaaaaa agaccccgcc aaagttgccg cctttatctc aaccgccaac
    10681 cgcctatccc gttaaagcaa caaaaattgc cgccggaatg a
//
LOCUS       DAXHRI010000054        10707 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXHRI010000054 DAXHRI010000000
VERSION     DAXHRI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.054"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..356
                     /locus_tag="ACFM75_001049"
     CDS             <1..356
                     /locus_tag="ACFM75_001049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255612.1"
                     /translation="IRCFISLLRESRGGADTTLVLVSVISSFFSSREYIDSRRQVLDT
                     AFSYKSDGRMVLFKSIKQESISFLSEDLKKDWMFHKVKTGEKDGYGSDEMLSVPRVYL
                     EMMSRKTGVPYSSIL"
     gene            complement(802..999)
                     /locus_tag="ACFM75_001050"
     CDS             complement(802..999)
                     /locus_tag="ACFM75_001050"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFC6255613.1"
                     /translation="MLKEGAAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNM
                     VCESFGRHYDTDENASSNIRK"
     gene            1585..2949
                     /locus_tag="ACFM75_001051"
     CDS             1585..2949
                     /locus_tag="ACFM75_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6255614.1"
                     /translation="MQHLTERMTQIAKLLNSSANNPDIDIPDFLTEIKDYSEFSVTDE
                     NGTYLHWDKFRRIHTEDTRMKWRAVKESRRKIRKPIDFPFEHQFWFCIPDSLQARLHL
                     IDKSCGSTIGTSSLGGFGRSEQNRFLLKSLIMEEAITSAQLEGAATTRKVAKDMLKSQ
                     RKPKTKDEIMIVNNYHLMKKAVELKNTPLSVEMILDLHRIATSNAIENKAEPGQFRQY
                     DEIFIADINGNSLYQPPPHGQVHTLMEEVCAFANNTYDGVENPFIHPVVQAIILHFLI
                     GYIHPFGDGNGRTARALFYWFMLKNGYWLFEYISISRLLKNAPAQYAKSYLYAETDDL
                     DLTYFIYYQCDIIKRAVADLEHYISDKQKHQQEFKAAIAQYTEKIGKLNQRQIGILQK
                     AVEESGKIFTAQEIANQYGISLNTARSDLSKLGEYRFLVPFKSGNALEYVAPQDLLER
                     LEKK"
     gene            3351..3815
                     /locus_tag="ACFM75_001052"
     CDS             3351..3815
                     /locus_tag="ACFM75_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255615.1"
                     /translation="MLHIPPTEPDRYVSSIVALNVHSSNGTGDWHSAAALNDDAYPQD
                     FYIYGKDQTRSTLHLLGNVGVIDGTNRFNEMGYYPENIPVWLADHPRACVDYLYTAVL
                     QTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWGNLQLWKRKNPIM"
     gene            3942..5231
                     /locus_tag="ACFM75_001053"
     CDS             3942..5231
                     /locus_tag="ACFM75_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFC6255616.1"
                     /translation="MMNSILTVSENRMRKPRITHLDVWANDERIGTLEKGAMYRFTYD
                     NPGSPLLGLHYQDRGKVYISNNMPHIFAQYFPEGFLDAHITSKYAFHDAPFEDNEMLR
                     LAILGRETLGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAISRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IPVAQTSLSEDSSVLLVRRFDVSEQEYFLGMEDFTSLRQYSAEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPTYDVLDTSIYR
                     IGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
     gene            complement(5556..7343)
                     /locus_tag="ACFM75_001054"
     CDS             complement(5556..7343)
                     /locus_tag="ACFM75_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6255617.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHARF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPSYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     LGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWTRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLLGQTLAGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
     gene            complement(7398..7856)
                     /locus_tag="ACFM75_001055"
     CDS             complement(7398..7856)
                     /locus_tag="ACFM75_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFC6255618.1"
                     /translation="MPITPPLNIISPKLYPNEQWNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILPVLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNSDWNCG
                     DNIWLIQWFAPFGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            complement(8022..8336)
                     /locus_tag="ACFM75_001056"
     CDS             complement(8022..8336)
                     /locus_tag="ACFM75_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255619.1"
                     /translation="MKKTLSNLVLISFCSTMLTACPFWGSCADWNCSKLNPRWEKAVD
                     TCETENLTFVLHELGNQLGYKSITEVSFSEIGQHISYQCIHHGGGYNIRKDKQYGHLF
                     VQ"
     gene            complement(8348..8647)
                     /locus_tag="ACFM75_001057"
     CDS             complement(8348..8647)
                     /locus_tag="ACFM75_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255620.1"
                     /translation="MKKKLSKYSLFLSSVFCLTACATTLLGTVVVGTALYGTSSNWTT
                     TDKEHQEIMNCLDKALVKLNIYFENDEEKYKNKTVMDIYYQCIKNPNYVVTHNNL"
     gene            complement(8628..9044)
                     /locus_tag="ACFM75_001058"
     CDS             complement(8628..9044)
                     /locus_tag="ACFM75_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HFC6255621.1"
                     /translation="MMADTMFAKISSMVICLQNDYADVLQKNGYTYMGADGKTYNSGS
                     YSIVHDKDFVGNKWIPFLLGTNDTTQGTCKGLCYSHSSYFAEVPKAGTKEFDDYVKIW
                     GEVEYDAQSKPINKSKPILVEPNKTKDNEKYEKEAF"
     gene            complement(8986..9300)
                     /locus_tag="ACFM75_001059"
     CDS             complement(8986..9300)
                     /locus_tag="ACFM75_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255622.1"
                     /translation="MKKILSNLMLISFCSTMLTACPFWGSCANWNCSELNPRWVKSVH
                     NCRSNNYKVVLSAIGSKFNYQSTSQVQDSDIGQHISGQCTYYDGRYDVRKDKQYGHLF
                     AK"
     gene            complement(9281..>10707)
                     /locus_tag="ACFM75_001060"
     CDS             complement(9281..>10707)
                     /locus_tag="ACFM75_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6255623.1"
                     /translation="LTGKTAAQTKADIGTNVTTDTAERHSGSLKNTFNREAVQNELDL
                     QREVTQEFDKTRQGVKQELYAVVDSKRAQATQERLKNGGYDNDKSRALNKEANELDEK
                     IRWLDAGLGLVWGAGSSDMAWSMFATTQADRAVRSATAPKEMWFHKKIIDEKTGKVSF
                     DTRQIWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAAKQNRNSTNGSGV
                     IAVMNPPTGKYKSDSNNKIKDFLWLSSSLVSELMYVGYDQLNNKVFQGYLPKTNSEKL
                     NQDIYREVQKMGNGWSVDTSNHSRGGITASVSLKDWVNNQKQNGIAPIRKARFYGTAT
                     NVQNDYADVLQKNGYTYTGADGKTYNSGAYSIVHDKDFVGNKWIPFLLGNNETTKGAC
                     KGFCYSHSSYFAEVPEQYKRDKNGNFVTDNEGNKIETKDWDSYTKIWGIPKKGTDGKD
                     INHAIPKLVNPNKPNGEKYEENPF"
BASE COUNT         3018 a         2224 c         2178 g         3287 t
ORIGIN      
        1 cgatacgctg tttcatctct ttgcttaggg agagtagggg gggggcggat acgaccttag
       61 ttttggtatc cgtaatatca tcattttttt cgtcaaggga gtatatcgac tctagaagac
      121 aggtattaga tactgccttt tcttacaaga gtgatggtag gatggttctc ttcaagtcaa
      181 tcaaacagga aagtatttct tttctgtctg aagatttgaa aaaggactgg atgtttcaca
      241 aggttaaaac tggggaaaaa gatggatatg gttcagatga aatgctgagc gtaccccgtg
      301 tctatttgga aatgatgtcg cggaaaacgg gagtccccta ctccagtatt ctttaaattc
      361 taagcagaag acttcttcgt cggtcttttt ttgttgtttg gtttgcatag agtaaaactg
      421 tacaactgtt gaagcataaa accagcaaga aggtaaaaaa gaaagaagca gttctttgga
      481 ttttagatgt taccgcaatt agtttccttt cctaaaattt gtttaaatta tttgcaatat
      541 taatataaac gagatattaa tgatgagaaa tcaaaaaggc ataatgaata tattttgtac
      601 aaaatatttg cagtatttaa aaatgttggt tcgtatatga aaagttaaaa atgccaaaat
      661 gtacagttgc taaactgtaa aactgctaaa gcaacaaaac ataaaaagga atgcaaggat
      721 gcgatcacta catcttttta ttccgaagcg tttatgattt tacggtcaac tgctactcta
      781 tgtgcctagc ttttcagctc cctattttcg aatattggag gaggcatttt catcagtgtc
      841 gtaatgccga ccaaaactct cacaaaccat attggttctt gtggcagcaa cacctatccg
      901 tttgttcaag cgaccacaag agtaacatga ttggctggta gcattggctt taatctcttc
      961 gatatgaact ccatttttag ctgcaccttc tttcagcata tgcagcagta gggatgaggc
     1021 aactatcttc tggtggagtt tgttgacttt aatctgtatg cactgtaggt tgagcagatt
     1081 caattttttg atacagatta tccggctttg ttcggcaatt ctgtttgcga acgtatagta
     1141 gagctgtcgt cttgcagctt tcaatttgcg gtaggtattt taccattcaa tgcgtagccg
     1201 ctcggtacgt ttgagccaaa tgttatcttc gccatttgta ccaatttgtt tttacattag
     1261 gctgtgtttt agtaatctat tgatttcaat tatttgcaag ggaaaagaca attattttcc
     1321 ggttaggaat aaacctatcc tgttgaatac cttaaagcca aatacgccta tcaacaccat
     1381 attaaaacac agcctttttt aatatagtag acacaatctt tccctattta tgaaggtgat
     1441 cgcttctttc agattcgtat tttaatgctt cctattttta taaaaattga ctagaatagc
     1501 tcaattatta aaaattgcgc gattttggta tttatcatga aaatttccag acctccggaa
     1561 tttaccctgt tgcaacagga atatatgcag catctgactg aaagaatgac tcaaattgcc
     1621 aagctgctga attcttccgc aaacaatcct gatatagaca ttcccgattt tcttactgaa
     1681 atcaaagatt attcagaatt ttccgtgaca gatgaaaatg gaacctacct gcattgggac
     1741 aaattccgcc ggattcacac ggaagatacg cggatgaaat ggcgcgccgt caaggagagc
     1801 cgcagaaaaa tccgaaaacc aattgatttc ccgtttgaac atcagttttg gttctgcatt
     1861 cccgactctt tgcaggcacg gcttcatttg atcgacaaaa gctgcggcag tactatcggc
     1921 acgtctagct tgggtggctt cggcagaagc gagcaaaaca gattcttgct caagtctctg
     1981 attatggaag aagcgattac atccgcccaa ctggaaggtg cggctaccac gcgcaaagtg
     2041 gccaaggata tgctcaaatc gcagcgtaaa cccaaaacaa aagacgaaat catgatagtg
     2101 aacaactatc acttgatgaa aaaagcggta gaattgaaaa atacgccgtt aagtgttgaa
     2161 atgattttgg atttgcaccg cattgctacc agcaacgcta ttgaaaacaa ggccgagccc
     2221 ggacaattca ggcagtatga cgaaatcttt atcgccgata tcaatggtaa cagcctgtat
     2281 caaccaccgc cgcacggaca ggttcatacg ctgatggaag aggtgtgtgc gtttgccaat
     2341 aatacctatg acggcgtgga aaatccgttt atccatccgg ttgtccaagc tattatcctg
     2401 catttcctca tcggctacat ccacccattt ggtgatggca acgggcggac agcgcgggct
     2461 ttgttctatt ggtttatgct caaaaacggc tactggctat ttgaatacat atccatcagc
     2521 cgtcttctga aaaacgctcc tgcccaatac gccaaatcct atttgtatgc ggaaactgac
     2581 gatttagatt taacctattt catctattac caatgcgata ttatcaagcg ggcggttgct
     2641 gatttggagc actacatttc tgacaaacaa aaacaccaac aggaattcaa agcagcgatt
     2701 gcccaatata ctgaaaagat aggaaagttg aaccaacggc aaattggtat cctgcaaaaa
     2761 gcagtggaag aaagcggaaa aatctttact gcacaagaaa ttgccaacca atacggcatc
     2821 tccctgaata ctgcccgtag cgatttgagc aaactgggag aatatagatt cctagtgccg
     2881 ttcaaatcag gaaatgcttt agagtatgtt gctcctcagg atttattgga aaggttagaa
     2941 aaaaaatagt ttgctagcct aaaatgcagc tttaaccgag tcaaaatcaa tacagtccgc
     3001 accttcaaaa agaagctgcg gactgcttgc tttttgctct acaaatgatc tttgtagctg
     3061 atttaaccaa gattgtagca attttgcttt ccgagcaagc agggttagaa aattcgatac
     3121 ttttaattat tggctgtgtt ttaatatgat gttgataggc gtgtttggct ttaaggtatt
     3181 caacaggata ggtttattcc taaccggaaa ataattgtct tttcccttgc aagtaattga
     3241 aatcaacagt ttactaaaac acagccttac attattgggg taactatcct gtaaaatatg
     3301 tcctaaaacg tggaaaccac ttttgctctg ctaaatttta aggaatcttt atgttacata
     3361 tacccccaac ggaacctgat cgatatgtta gcagtattgt agccttaaac gtgcatagtt
     3421 ctaacggtac aggcgattgg cacagtgcag cagcccttaa tgatgatgct tacccccaag
     3481 atttctatat ttatggcaaa gatcaaacac gtagcacgtt acatttgctt ggtaacgttg
     3541 gtgtgattga tggaacaaac cgcttcaatg agatgggcta ttatcctgaa aacatcccag
     3601 tttggctcgc agatcacccc cgtgcttgcg tggattatct ttacacagca gtgttacaaa
     3661 ctggctcaat cggtcgggtg attttagatg attggtttcc aagcgatgaa gacaagcaat
     3721 cagtttatga cttacttaac caaatcgaac cgcacttgaa tactcaagaa tgggggaatt
     3781 tacagctatg gaaacgcaaa aacccaataa tgtaatgcta accgaacgag ataaaaggca
     3841 cgagaagcgg tattgctgaa tttcatccgc aatacgccat gtacaattaa atcagcagat
     3901 gtgtctaaac gtcccgctgt tcccgtttga aagaatttct catgatgaat tctatcctca
     3961 ccgtatccga aaatcgtatg cgtaaaccca gaatcaccca tttggatgtt tgggcaaacg
     4021 atgaaagaat cggtactttg gaaaaggggg caatgtaccg tttcacatat gacaaccccg
     4081 gttctccatt gttgggtttg cactatcagg ataggggtaa agtatatatc agcaacaaca
     4141 tgccgcatat ctttgcacag tattttccgg aaggcttttt ggatgcacac atcacaagca
     4201 aatatgcttt tcatgatgcg ccttttgaag acaatgagat gctgcgcttg gcaattctgg
     4261 gcagagagac tttgggtcgg atacatgtgc gctgtaatga cccgcttttt aatgaatgga
     4321 ttgacgggtt ggagatgaaa aatccaagaa tattgactga acgggatttg ctgggcataa
     4381 atgcccgaca ggtttttcag caatatatgg cagaaatctt ccatcacggt cgtttcgtca
     4441 gtgtatccgg gatacagcag aagatgtcct tagatgccat cagcagaaat accaagcaaa
     4501 ctgcctcata tattgccaaa ggttttgatg catccgaata tccttgcttg gctgccaatg
     4561 aatttttatg catgcagacc atcaaacaag caggtattcc ggttgcacaa accagcctgt
     4621 cggaagattc atcagtctta ttggtacgtc ggtttgatgt cagtgaacag gagtatttct
     4681 taggcatgga agactttacc agtctgcgtc agtattcagc agaagataaa tataaaggca
     4741 gttatgcggc tattgcacag attatccgac agatatccgg caggcccgat gaagatttaa
     4801 tccatttttt taaccagctt gccgccagtt gcatattgaa aaacggggat gcacacctta
     4861 aaaatttttc agtactctat catgacgaat acgatgtacg tcttgcacct acctatgatg
     4921 tattggatac atcaatatac aggattggaa cacaaggaat ttttgatgct tatgacgata
     4981 cgctggcatt aaacctgact aaccacggta agaaaacata tccttccaag aatacattgt
     5041 tggattttgc tgagaaatat tgcgatttgg gaagagaaga tgcatccttt atgatagata
     5101 caatcgttca agctaaagaa caggttcttg ttaaatactc ggatgtattg cgtgagaatg
     5161 aatggttggc gcagaagtgg cattttatcc cggatgaaaa tgaagaaggt ctaccgttta
     5221 cattccggta gctgccgctg tcagagatgg ccggtcgact tttaccctga aaatcacttc
     5281 atcttatggt gtttgaaacc gagaaattag aagaatcgta ttcggtagga gatatactgg
     5341 gaagattgga aaactggtaa atcactctat tgattgagtt ggcggcctat atgtttaatg
     5401 taggcaaacg agaaggaatc atgtatttca tgattccttc ttaaattcct gtgtcaatct
     5461 aatatcaaaa cacagccatc tcttaccata atcatgatag gtgttttatt atgaaaagct
     5521 atatctatag ttaccgttga tttgactatg ccgttttaaa acgtatagcc tacctgaaaa
     5581 ccgcttgccc atttccttga ttggaaaaat tcgggctttt tcaatgcgcg gccggtaaat
     5641 atatcgtaat gcaggttgcc gccaagcttt atctgcccgc gtatcccaat tgctgtgccg
     5701 gctagagttt ggcccaataa ccatttggcg gattgtcctg aaacatgtcc tacatcagcc
     5761 ccaagataaa gctgatggcc tggtttaaat tgccagctca aatcgttgcg ccaataccat
     5821 ccccgctcgg cagacaaact catttcaccg tcgaagccac gcacggtgtg gtgtccaccg
     5881 atagccagtt tgtcttgcga tgttagcggg gttttgttcc attgtgcatg aacggatgtg
     5941 tcataggcaa atagctgttt accgatttga aaaggagtat ttacatcagc cgatgccgtc
     6001 caaattttca tacgtgacgt gccttctcca aaggcttctt caggcgcgcg cagagcatct
     6061 ttcatgccgg tgccgcgttt atatttcaac ttaaaatctg ccgtactgcg gccgatatat
     6121 tctttgtggg aaagttctgc caaccaaccc gcagttttac gccgttgtac agtcagttcg
     6181 gcatcatcaa tgtaactttt tgtttccctc gtccacagtt ttacaccaag ataggttttg
     6241 cgtttggcat cacgatacaa caggcggttg aagccgaaat cagtattgta actttttcca
     6301 ttatagtcat agacttccga taatccggaa accgcctgat ggtaacggta gccattgtga
     6361 ttgaatgccc atgtccattt accgaaaggg gctgaataat gtacggcgta attgtttgat
     6421 ccgccttctt tgcgatggcc gtcaaaactt tcctcatcgg gcgtaccgcc aattgaacgt
     6481 ccataattta catagaacat atcactcagt cccaaaggat tgtcggcaga gaaagtgata
     6541 tttccttggt attttcctgt cgcctcacta cccgaattat ccatccccac actcacacgg
     6601 tagggcagca gacgttgccg ccattgcacc acgacatcac tttggtttgg ttctccctct
     6661 acgggaacga tttggagatc ggcttccgca gtcgggagac gtttgagatt ttccagtcct
     6721 tgttccaaat cacgcagatt caacagatcg ttcgagcggg tgggaaattt gttctggaat
     6781 gctgcaatac gtcctgcatg ggtttgatca tcgttagacc gatcgattcg tatggagcgc
     6841 agatagctcg gtatcagggt taattgaagc ttgccactat tcaaatcctg tggcgcagcc
     6901 aagatacggg tcgtggtata tcccctgccg atcaaagcat tttgtgctaa ggacatgatt
     6961 tgattaatgt tgcccgcatg cagacacttg ccagcctgaa aacccgtttc gcgcaaggca
     7021 cgttttaggg caaactgaaa ccgagcatgg tgttcgcctt ccaacaccac ttcgttaatg
     7081 gcaaaacacg gttggctgct gtcatcgccc atcaactgat taaccgtttc tcccgtgttt
     7141 ttttgatgca aacgcacatc gctttcaggc tgcatggttt ggcgcaactg ctcttcgcgt
     7201 tggcgttgct gaatatcttg ctgcatacgg atttcggcag ggttggggga ggccaacaaa
     7261 gtagcaggag caatgatacc tgccaataag cagcaccaag acaaaaagcg aatattaggc
     7321 aaataggata aaggaagttt catggcatgg tcgcaaaatc aataaaatca tcaatgggca
     7381 ttcaatacac gattgcatca agttttaaaa gttaaaattc tcaaaccctt atcgctccct
     7441 ttatgataca aggcgcgtac tgtcgtacta ggaaataact ggcgcacagc tgagcgcatt
     7501 tgatgactgt gtccaaatgg cgcaaaccat tgaatcagcc aaatattgtc gccacagttc
     7561 caatcgctgt tgtcacgcaa atggcggtca gattctaaat aatgcgcctg cgccacttca
     7621 tcaaaataag cccatgagat ataaccgatt ggttgggtac ccttgcaaaa caaagcgaac
     7681 tgcccgtttt ttaacacagg caatatatac gtcatcatct ccacaatagg tacttggcga
     7741 tgcgtaggcg actgatacca tagccaagtg atggcaccga gtgcttcgct ttcgttccat
     7801 tgttcattgg ggtagagttt aggggagatg atgtttaagg gtggagtgat gggcatattt
     7861 taagttaggg ttcgtgttgg ttaaaacaaa aaatggtttc agcctgaaat gaaatatttc
     7921 acgttaaaac atagttttag cacatgggac tgaactgcgt gggcaacgag ttgcccaccc
     7981 taccatgtaa cagttcgtat taattgcaca ggctacgctg gttattgcac aaacaaatga
     8041 ccatactgct tatctttacg aatattgtat ccaccgccat ggtgaataca ctgataagaa
     8101 atatgttgcc caatttcact aaatgagact tcggtaatag atttatatcc taattgattg
     8161 cctaattcat gcaaaacaaa ggtgagattt tcagtttcac atgtgtcaac ggctttctcc
     8221 cagcgtggat taagtttgga gcaattccag tctgcacaac taccccaaaa agggcaagct
     8281 gttagcattg ttgagcagaa tgatattagc actaaattag aaagggtttt cttcatattt
     8341 ctctccttta taagttattg tgtgttacaa cataattagg atttttaatg cactgatagt
     8401 aaatatccat tactgttttg tttttgtact tttcttcatc attctcaaaa tagatattca
     8461 gttttactaa tgccttatca aggcaattca taatttcttg gtgttcctta tcagtagttg
     8521 tccagttact actagtaccg tacaaggctg taccgactac gacagtacct aataaggtag
     8581 tagcgcaagc tgttagacaa aaaacagatg aaagaaacag agaatattta gaaagcttct
     8641 ttttcatatt tttcattatc ttttgttttg tttggttcta ccagtatggg tttagatttg
     8701 ttaattggct tactttgagc gtcatattca acttcacccc atatttttac atagtcatca
     8761 aattcttttg tacctgcttt tggcacctcc gcaaaataac tgctatgcga atagcacaac
     8821 cccttacatg taccttgtgt ggtgtcattg gttcctagca agaaaggtat ccatttgttc
     8881 cccacaaaat ctttatcatg cacgattgag taggatccgc tgttataagt tttgccgtct
     8941 gcacccatat aggtatagcc gtttttctgt aaaacatcgg cgtaatcatt ttgcaaacaa
     9001 atgaccatac tgcttatctt tgcgaacatc gtatctgcca tcatagtaag tacattgtcc
     9061 agaaatatgt tgcccaatgt cactatcttg aacttggctt gttgattgat agttaaattt
     9121 acttcctatt gccgataaca caactttata gttatttgat cgacaattat gaactgattt
     9181 cacccagcgt ggattgagtt cggaacaatt ccagtttgca caactgcccc aaaaagggca
     9241 agctgttagc attgttgagc agaatgatat tagcattaaa ttagaaagga ttttcttcat
     9301 acttttctcc atttggcttg tttggattga ctaatttggg aatagcgtga tttatgtctt
     9361 ttccatcagt acctttttta ggaattcccc atatttttgt gtaactgtcc cagtctttcg
     9421 tttcaatttt attcccttca ttatcagtaa caaaatttcc atttttgtct cgcttatact
     9481 gttcaggcac ttccgcaaaa taactgctat gcgaatagca aaatccctta caagctcctt
     9541 ttgttgtctc attatttcct agcaagaaag gtatccattt gttccccaca aaatctttat
     9601 catgcacgat tgagtaggct ccgctgttat aagttttacc gtctgcaccc gtataggtat
     9661 agccgttttt ctgtaaaaca tcggcgtaat cattctgcac atttgtggct gtaccataga
     9721 aacgtgcttt tctgattggg gcaatgccat tttgtttttg attgtttacc caatctttta
     9781 aggaaacgct tgctgtaatt cccccacgac tgtggttact ggtatcaacc gaccagccat
     9841 tacccatttt ttgaacctct cgataaatat cttgattcag tttttctgaa ttggttttgg
     9901 gtaaatagcc ttggaacact ttattattta attggtcgta accgacatac atcagttcag
     9961 aaacaagact tgaactgagc cataaaaaat cttttatttt gttattagaa tcagatttat
    10021 atttccctgt tggaggattc ataactgcaa taacaccact accgtttgta ctattacgat
    10081 tttgttttgc tgcgttgctt aatgaatctt ctcgattatt gaaaatacca ggattagaca
    10141 cagtaataac tttgccattt gtgtcttgaa tgcttgccag ttcttccttg cttaaatcat
    10201 tcaatgacca aatttgtctg gtatcaaagg atactttacc tgttttttca tctattatct
    10261 tcttatggaa ccacatttct ttaggtgcag ttgcagacct taccgctcta tcggcttggg
    10321 ttgtggcaaa catactccaa gccatatcag aactccctgc gccccaaacg agtcctaatc
    10381 ctgcatcaag ccaacgaatt ttttcatcta attcattagc ttccttattt aatgctctag
    10441 atttatcatt gtcatatcca ccatttttga gtctctcttg tgtggcttgc gctcgtttgc
    10501 tatctactac tgcataaagt tcttgtttca ctccttgtct agttttatca aactcttggg
    10561 ttacttccct ttgcaaatcc agctcgtttt gcaccgcttc tctgttgaag gtgttcttca
    10621 agctgcccga atgtcgttcg gcggtgtctg tggttacgtt tgtaccaata tcggctttgg
    10681 tttgtgccgc tgttttgcct gtcagcc
//
LOCUS       DAXHRI010000055        10707 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXHRI010000055 DAXHRI010000000
VERSION     DAXHRI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.055"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(1..489)
                     /locus_tag="ACFM75_001061"
                     /pseudo
     CDS             complement(1..489)
                     /locus_tag="ACFM75_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(646..2652)
                     /locus_tag="ACFM75_001062"
     CDS             complement(646..2652)
                     /locus_tag="ACFM75_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC6255624.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNS
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSIRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGVIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2941..4968
                     /locus_tag="ACFM75_001063"
     CDS             2941..4968
                     /locus_tag="ACFM75_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC6255625.1"
                     /translation="MSLSEFIERRTSFNLMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(5060..6613)
                     /locus_tag="ACFM75_001064"
     CDS             complement(5060..6613)
                     /locus_tag="ACFM75_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC6255626.1"
                     /translation="MNRTYANFYGMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPTGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            6664..6804
                     /locus_tag="ACFM75_001065"
     CDS             6664..6804
                     /locus_tag="ACFM75_001065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255627.1"
                     /translation="MPSENGRRQFIIFAFRRHIERRLCAVCRLRTNPPCRANRARGGY
                     AV"
     gene            complement(6817..8007)
                     /locus_tag="ACFM75_001066"
     CDS             complement(6817..8007)
                     /locus_tag="ACFM75_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC6255628.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILAYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGVCSMKNWLNHGWAAKDGVHFSSKGYQRSAEMLADSLEELVRA
                     APKR"
     gene            complement(8004..8987)
                     /gene="patB"
                     /locus_tag="ACFM75_001067"
     CDS             complement(8004..8987)
                     /gene="patB"
                     /locus_tag="ACFM75_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC6255629.1"
                     /translation="MKHFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYAFSDGIKTFLSGETPPTAQDGGSADMPSEAATSEAVPQTGET
                     EWKQDTDTAAVRSGDKVFFVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGKDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(8998..10434)
                     /locus_tag="ACFM75_001068"
     CDS             complement(8998..10434)
                     /locus_tag="ACFM75_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC6255630.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMSMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVFAPSGIPGFIYANF"
     gene            complement(10564..>10707)
                     /locus_tag="ACFM75_001069"
     CDS             complement(10564..>10707)
                     /locus_tag="ACFM75_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFC6255631.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGITGMLAAAAGLWLCLNLNRH
                     IQT"
BASE COUNT         2234 a         2589 c         3305 g         2579 t
ORIGIN      
        1 taatgtggtt ctgacggtct gcacactcct tggaatggtt gatgcggtaa tggataaaac
       61 cgctcacgtc caacttgtcg cagctgctca gactatcggt ataaacaatg ctgtccggca
      121 tgattttctt tttgatgaca gggagtaacg tttcagactt ggcattatcc accacaacgg
      181 tatagacccg tccgttgcgt ttcagaatgc cgaaaacaac cacttttcct gctgcaccgc
      241 gaccacgtct acctttatgc cgtccgccga aatcgctttc gtccggctcg acagagccct
      301 caaaaacctc atcggcagcc aaggccaaat gatggttgat aaccgtgcgg attttacggt
      361 agaacagtgc tgccgaattg ggatggatac ccaaaatatc ggcggcagaa cgggcggtaa
      421 cttccagtac aaaaaacgga gcagttcttt ctgtactttt ttctttaatt tgcagtgcgt
      481 tatcttcata tttcgagggt aacatatctg ctaatctagt acagcccctt ttaaaatgcc
      541 gtctgaacca cgtttcggtt cggacggcat ttttgcatcc ttttatgggg atttcccgca
      601 tcagggcgga ttcggggcga atcccgcttc agacggcatt gccggtcaag gcttgtccga
      661 aggaggatgt ccctgttctt tggcgggcgg gaggaaaagt atgcagggat tgcttttaat
      721 ctgattccaa aaacttttaa tcagattgcg gatgctgccg attttcgtgc cgcgcgagcg
      781 cagggcgacg aagagggcga ggctgaaact gacgcagagg ttgaccaggc cgatggcgag
      841 gacgctgaaa atgccgagta cgaacgtgcc caaaccgacg ttgccgctga cggcggcata
      901 gccgaggttg gcggaggaaa aggcgacgtg gcggatgtcc agcggcagcc cgaggaggtg
      961 tccgaaatag ccggtcatac ccaagagcat cccgaagatg aagttgccta ccaacgagcc
     1021 gtagtgtttg tggatgtagg cggcgaggac gcggcggggc ccggggcgca tgattttacg
     1081 cagcaagggg ttgaaaggca ggcgttggcg caggttgagg tagtcggcgc ggttgtcgaa
     1141 aaaacctgcg atgatgccgg agcagaacag ccacagacct gcaatggcgg cataccacag
     1201 cgtcgggtaa gcgatgatgt ctatggattt gaactggtag gcggcggtgt gggcatcgag
     1261 tatgggctgc cggtacagat gggcatagcc gaacgatatg gcgcacgcca aaaggatggc
     1321 gatggaaacg ttgccgaaga cggcgacact ttgggagcgg catacgtcga tgaggagctt
     1381 ggcgagcttg ttgtccaccg ctttgccgcc ttcgttgaga tcgacctgtt cggcaaagct
     1441 ggcggcagtc atcgcgggct gcttggtggc gacggtgcag tgcagcatat ggatgatcat
     1501 aaagccgatg ccgtagttga acccggccga caaggaagtg aggaaggggc tgaggccgag
     1561 tgtgccgatg cggattttga gcagcgccat cagggcgatg atgacgccgc cgcctgccgc
     1621 cgagtagaac attttgaaat attctttgcg gttgcgggtg atgtagtgtt cgccgtggtg
     1681 gcttttgttt tcggtaatgc tgcgggcgag catacggatg ctgctgcggc gcaggtggcg
     1741 ggtgctgtat tgttcgaccg ccgcgtagat gagggagttc atcagcgcga tggtcaggcg
     1801 gatgctgttg ccggcgccgg tttggatgtc ggtgagcagc ttcagacggt ctacggtctg
     1861 ccggagccgt tcgagcaggt gggcgacttt gaccgacgaa ccggagccgg cgcccgtccc
     1921 tttgcggcgc aaatagtcaa tctggctgaa acattggtcg aacatcacct cgaggtgggc
     1981 ggtgtcgtaa ggcgcagtgc tgttgcggta gtgttcgacc agtttggcgg tttcgcgctg
     2041 gagggcgacg aaggaagaat cggcttccag caggcgcggg gcgatgcgga tgaggtcggg
     2101 ttcgatggct tcggatgccg tccagacaga cagcatttcg atggcgcgca aacgcgcatc
     2161 ggcaaggcgg atgcccgccg tctgcaattt ggacggctcg gcgtggctcc ggatgagttc
     2221 gtataaaacc agccattggc ggatattgag cgtttgcagc catttgtcgt cgttttcgga
     2281 atggaaaaga taaaggaaga cttcgcccaa gttggcaaaa tctttatatg acgggctgaa
     2341 gcgttcgtag atgcgtatgc ccatttcccg ggcaaagctg tggcgcgaga agatgccgag
     2401 tttgatgagt gccggataaa tgtgtatttg cgcgagccag atgtaaaaac accggctgaa
     2461 gcgggacagt aattccctgt cttgtttgaa tgtgtcgata atcaggtcga aacgttcccc
     2521 cgcacatttt ttgccgccac ggcgcaaaaa tttaatcagt gcgttgagga cgttgacaaa
     2581 atcggtatgt cccaagcttt ccgaaagcag ggggcgcagg ttttgagggg taattttctt
     2641 catatcagcc attgtaccgc atcggcaatc cggcgttaaa cgccccgata gcggggaggg
     2701 cgcgcctgcg gtgggcggcg cattttccgc cggtttgaaa tcaaactata ttaatgatat
     2761 aataaatatc gataaatcaa tctgatagaa taatccgtat tgatttttaa ttttttcgat
     2821 tataacaatt tcggccggtt tgtgcatttc gtcatccgga agccggtatt ttaccggcag
     2881 gttttgaccg ctttgcccga tgaccgggtt tgtctgtttt taacaaagga gtgagaaggt
     2941 ttgtccctgt ctgaatttat agaacgccga acgtcattta atctgatggt tattttgacg
     3001 actttgtttt ttgtgtgtgt tttggtggta ttggttttaa ccgtgccgga tcaggtgcag
     3061 atgtggctcg atcgggcaaa agaagtcatt tttaccgagt tcagctggtt ttatgtttta
     3121 acgttttcca tttttctggg tttcctgctg atactctcgg tcagtagttt gggaaacatc
     3181 aggctcggac gggatgaaga tgtgccggaa ttcggcttcc tgtcgtggct ggcgatgctg
     3241 tttgcggccg ggatgggcgt gggtctgatg tttttcggcg tggcagagcc gttgatgcat
     3301 tatttttcgg acattacggc cggcacgccg gaacacaggc agcagcaggc attgctgcac
     3361 acggtgttcc attggggcgt tcacgcttgg tcggtgtacg gtacgattgc attggctttg
     3421 gcttatttcg gtttccgcta caagctgccg cttgccctgc gttcttgttt ttaccccctg
     3481 ttgaaagaaa aaatttccgg aaggttcggc gatgccattg atattatggc gttgcttgct
     3541 acttttttcg gcatcatcac tacattgggg ttcggggctt cgcaactggg cgccggattg
     3601 caggaaatgg gctggattgc cgaaaacagc ttcagcgtgc aggttttgat tatcgccgcc
     3661 gtcatgtccc tcgccgtcgt ttcggcaata tccggcgtgg ggaagggcgt gaaggtgttg
     3721 agcgagttga acctgagcct tgcgtttttg ctgctgtttt ttgttttggc ggcgggtccc
     3781 actgtttacc tgttgtcggc attcggcgac aacataggga actacctcgg aaatctggtg
     3841 cgcctcagtt ttaaaactta tgcgtacgaa cgggaacaca agccgtggtt tgaatcttgg
     3901 acggtgcttt attgggcgtg gtggtgttct tgggcgccgt ttgtgggttt gtttatcgcg
     3961 cgcatttcaa aggggcgcac catccgcgag tttgtcttcg gggttttgct catccccggc
     4021 ctgttcggcg ttttgtggtt taccgtcttc ggcaatacgg cgatttggct gaatgacggg
     4081 gttgcggggg gaatgctcga aaagatgacc tcctctccgg aaacgctgct ttttaaattc
     4141 tttaattacc tccccctgcc cgaattgacg agcatcgtca gcctgctggt catttctctg
     4201 ttttttgtaa cttctgccga ttccgggatt tatgtcctga acaatattac ctctcgggac
     4261 aagggcttga gcgcaccacg gtggcaggcg gttatgtggg gcgtgctgat gtctgccgtt
     4321 gccgttttgc tgatgcgctc gggcgggcta ggcaacctgc agtctatgac cctgattgtt
     4381 tccctgccgt ttgccctgct gatgctgata atgtgtttca gcctgtggaa aggattgagt
     4441 gcggataaga aatattttga gacccgggtt aaccctacca gtgtattttg gacgggcggc
     4501 aagtggaaag aacggctggt gcagataatg agccagacgc aggagcagga tattttaaaa
     4561 ttcctcaaac ataccgcatc gcccgctatg cacgagttgc aacgggagct ttcggaagaa
     4621 tacggcttga gcgtccgggt cgataagatg tttcatcagg acgagcccgc aatcgagttc
     4681 gtcattcgga aagagacgat gcgcgatttt atgtacggga ttaagtctgt cgggcaggat
     4741 gtatccgacc agttgattaa cgacggcaag ctgccgcata tccggcatca gacaacttac
     4801 aaaccctacg cttatttttt cgacgggcgc gtcgggtacg atgtgcagta tatgaacaag
     4861 gacgagctga ttgccgacat tttgaaaaac tacgaacgtt atttgatgtt gttggatgat
     4921 gtcggtcagg aactgatggc gcacgagcag gtggaattgg cagagtaagt gccttatctc
     4981 cggtgtttgc caaaaaaaat gcggtgaatc agattcaccg catttttttt gccggacggg
     5041 gcttcggacg gcacggcgtt catttgtttc cgtcaaactg ctccttcaac acccgtttca
     5101 ataccttgcc cgtagcgttg cgcggcagcc cgtctttaaa gtggatttgt ttggggattt
     5161 tgaaatttgc cagcacggta cgcaggtggc ggcggatttc gttctcgccc aaatccatac
     5221 cttccttcaa ttggacgaag gcgacgattt cctcgtcggc ataacggtct ttcacgccga
     5281 tgacggcggc ggcttcgacg gcatcgagtt tgtagatttc ttcttcgatt tcgcgcggat
     5341 agacgttttg acctttggaa ataatcaaat cttttttgcg gtcgacgata aagataaagc
     5401 cgtcttcgtc tatggtaacg aaatcgcccg ttttcaacca gccgttgacg atggtttcat
     5461 cggtggcggc aggcatattg aggtagcccc gcatcaccga accgcccctg acgatcagtt
     5521 cgcccacttc gccgcgcggc acttcgacca attcttcatc gacggctttg gcttccaaac
     5581 cgggcagggg gatgccgacg ctgcgggctt tttgcctctc gggcgtattg acggcgacga
     5641 cgggagaggc ttcgctcagt ccgtagcctt ccagcaattt ggcgcgaggg aacttggctt
     5701 tgaaatcgag gatggtttgt tccgccaaag gcgcaccgcc gctgataaac aggcgaatgc
     5761 ggttgaacca tctgaaatac caagggattt tcgccttgct cattgcggtg taaatcgcgg
     5821 gtatgcccaa aaacacggtc gcgcgtttga gcagggcctg tttcaaaacg ttggaaaacg
     5881 gaaaaacgga tttgaccaaa ataatcgaac acgccatata aatcggcagc agcaccatag
     5941 ccgtcagcgt gaagctgtgg aacatcggca ggaaaacgat aaagcggtcg cgtttggaga
     6001 ttttaaagat gcgttcgatg ccgttcaggt tggcgaacag gttggcgtaa ctgattagcg
     6061 cgcctttggg atgccccgtc gtgccggagg tgtagatgat gtgtgccaaa tcatttatcc
     6121 ggggttggcg gcccaagtcg ggtttttcgg ggaagcggcg cacgtcttca aaaaaggcat
     6181 cgccttccgc catttcgccg gtcggacggc ttttgtccgt ccaaatgatt ttttcgacgg
     6241 gcgtttgcgc cttcaagccc gccaattctt ttgacaggcc ggccgaggcg aacaggaagc
     6301 gcgccttgca gtcgttcagg atatacgcgt attcgctgtt tttcaaaaat gtgttcatcg
     6361 gtacggcaac cgcgccgatg gcggatacgg cgaaataggc ggtaataaat tccgtggaat
     6421 tggaaaccgc cagcgcgacc gtgtcgccga acttcacgcc gatattttgc agatacgccg
     6481 caacggcttc ggcttcctgc ttgagcgcgc ggtaggcggt tttttccttg ccgtcgaaca
     6541 ctgccgtgcc gttgccgttt ttgcggcagg cggcggcgag cattccgtag aaattggcat
     6601 aagtccggtt catgaaactt cctttatggt tgcggacggc gaacgccctc atttgaaaac
     6661 aatatgccgt ctgaaaacgg aaggcggcag tttatcatct ttgccttcag acggcatatc
     6721 gaacgccgcc tttgcgccgt atgccggttg cggacgaatc cgccttgccg ggcaaaccgt
     6781 gcgcggggcg gctatgccgt ctgaaagcct ttttgtttac cgtttcgggg cggcgcggac
     6841 gagttcttcg aggctgtcgg cgagcatttc cgccgaccgt tggtagcctt tggaggagaa
     6901 gtgtacgccg tctttggcgg cccatccgtg gttgagccag tttttcatac tgcaaacgcc
     6961 gcccatcgcg ttttgccaag accagaacat cgtctgcccc tgccgtgcga cgcgccgctg
     7021 catctgttgg acttcggtca ggcggacggg gcgtgtgccg catacgccga gcgtgttttt
     7081 cagggattcg ggtgcgccga tgatgaggat gccggcggca ggcaggctgt cgcggatttg
     7141 gcggacggta tccagccatt tctgttcggt atcggcaatg tcgatgttgt cgccgaaggc
     7201 ttcgttggta ccgtaggcaa ggatgactag atcggcgccg gtttgggcaa ggtcgttcat
     7261 acggtcggca cgccatttcg accactgggt taattgtgcg ccgttgatgc ccatcgcgga
     7321 aacggtaatg ccgccggcgg gattttcgat attgataaat ccgatgtccc acggcatttc
     7381 ggtgtgtatg gtcaggggca gtgccgcgcc cgtatccagt acctgccagc cgccgccgtt
     7441 ggcggagacg gtgttgccgt tgacggtcag ggtttgttcg gcgagcaggg gtttggcaaa
     7501 cagggaaaca cgctgtttgc cggtcttgcc gtccgatgcg gtcagggtca tgctgccgcc
     7561 gctgccggtg tgggcgagga tgccgccgag cgggaagtct ccgctattgt tcctgctggt
     7621 gaggctttgc cagttaccgt tgtgccggac ggccgccatg cgctgccctt tgacgttggc
     7681 ggggtaaacc cagcctatgc cgccgtcgcc ccaagttttt tgcaggcgtt tgcgcaggct
     7741 gtcggtaaag aagtcgccgg cggtatgcga gtcgccgatt tgcaggatgc ggaaggtctc
     7801 gccgctgcct tgtgcgacgg attggagttt tttcatccaa ggcgaggcgg cggcattgcc
     7861 gtagtcggtc aagagcccgt ttgtatcggt gtagggggcg gacggggaaa cggtaacggt
     7921 gtcgaggctg acggaggcga cgggcagggc ttctgcctgc gtggcggcga acagggcgga
     7981 aaatgcgatg aggtgtttgg ggttcatggc tgtgtacttg atggttgcgt acttggttca
     8041 aaaacgattt tttccattat ttttgccgcc agcagttttt gtccttcggc ggtaaagtgt
     8101 atgccgtcct tgctgcggta gcggacgggt ttgccgttga cgttgacgga gtccgtgtag
     8161 cggtctttcc cgccgctcag ggtgtgcgcg gtgggaatca ggatgatttt gcctttcaaa
     8221 tgttccgaaa gcagtttgtc gaggtagcgc atctgcccgt cgagcttggc ttttttcatg
     8281 taggggatgc cgagccagac gacttggacg cggtgcgtgt gtgcggcttc aaggatgcgg
     8341 tcgacgcgtt tcaggtattc ttgcgcccat tcgtcggaag cgaatttgag gtagcgtttg
     8401 ccgacgggga aatcccacgg gtcgttcggg ccgaggaaga cggcgagcac gctgatttcg
     8461 ggatgttttt gcagggtttc ttcaatcgtt ttcggccagt cgaagaatga ggggtaggac
     8521 agccccgtgc tttgtttgct gaggttgacg gattcgatgc cgtattgctg tttcaggctt
     8581 ttttgcacga agggggcaac gccctgcatc agcgagtcgc cgacaaaaaa gactttgtcg
     8641 ccgctgcgga cggcggcggt gtcggtgtcc tgtttccatt ctgtttcacc ggtttgaggg
     8701 acggcttcgg atgtggcggc ttcagacggc atatctgccg agccgccgtc ttgagccgtc
     8761 ggcggcgttt cgccggacag gaatgttttg atgccgtctg aaaaggcgta ggcgttttct
     8821 tgcaacgccg caccgctccg ccaccatccg taggcggcaa gcggttcgag cggactgttg
     8881 cggtggtagg tctgctgcca gtaggcgttg atggggtttt ggctgaacca cacggcaatc
     8941 agggcagaca tcaatatgga agtgaaaagg gaaagaaagt gtttcatgtc caaaccctta
     9001 aaaattggca taaataaagc cgggaatccc cgagggggcg aatacgataa tcagcagcag
     9061 gacggcggaa acggggataa accacagcca catcgggatt ttttccaaac ctttgaccgc
     9121 gccgtcgaaa gcgcgttgca ggtaagggta gagcagcatc acggatgcaa acgaggcaag
     9181 cagcagcata tccgcccgtt gcggcgcatt ccagccgttg gcattggcaa agagggcatt
     9241 gaaaactgcg cctgcatcgt cggggtttgc ggtattgaag acgacgaagg tcaggcagac
     9301 gaaatggaag gtaacaaacc acgagagcgg cgcaaggtat ttcagacggc atagtgcgtt
     9361 gcgtccgaaa tagcggtcgc ccgtgttgag cagcaccagt gccgtgccgt gcagcgcgcc
     9421 ccaaatgagg aagttccagc cgtagccgtg ccagatgcct gagagtacca ttgccgccat
     9481 caggttgagc tgtgtccgta aaaagccttt tttgctgccg cccaagggga tgtagatgta
     9541 gtcgcgtatc caggtggaaa ggctgatgtg ccatttgtcc caaaatgcac ggatattcgc
     9601 ggcacgaagc ggtgctgaga agtttttggg cagtcggaag cccagcagca ttgacatgcc
     9661 gataaccaaa tcggaatatc cggaaaagtc caggaagagt tggaaggtat agccgtacac
     9721 gccgcccaat acgccccagc cgtcgaattg ggcggggttt tcaaatacgg gcgataccca
     9781 gttttccgcc agcatccccg ccagccacca ttttttggca atacccagca gaatcaggga
     9841 aacggcgagt gcgggacgga cgggcgaacg cggttggcgg gtacggattt gcgccaatgc
     9901 gcccgcctgt tcgccgtccg cgcttttgaa tgcggcggcg cggataatcg ggccggaggt
     9961 aacggtgggg aaaaaactca ggtgcagcag cagttcgtgc cactcgaaac gcgcggcgtg
    10021 cggcgcacgg aagcagtaaa ccaggtaggc gattgactgg aaggtgtaat acgaaagccc
    10081 cagcggcatc aggatgtcga ttgcgccgcc ttgtccggca tattgtgcaa tcagcgggcg
    10141 gaaaaagtcg aaatatttga aaaagcccaa gacggtcagc gagacggcaa tgccgcaccc
    10201 cagccaaaag cggcgcgtat tttcgcgatc ggaacggagc agttcgccca aaaggtacac
    10261 gcaggaggaa taaaggacga cgattgccgc aaatacgggg ccgatatggt agagccagcc
    10321 cataccggca gccaaaagca gcaggttttg gacggacggg tatttcgcca agccccagta
    10381 aatcggcagg aaggcgagaa agaacagtgc gaactcgaca gacagcagcg gcataaacca
    10441 tccttggaat aagaaaatag gcgttaaaac acggcaaagc ccattatttt aagcaggggc
    10501 ggatatttct tcaatcaaaa tgccgtctga agggcgggag gggcttcaga cggcatcgcg
    10561 cggtcaggtt tggatatggc ggttcaagtt caggcacagc cacagcccgg ccgccgccgc
    10621 aagcataccg gtgatgccga tacgggaaat gccgacatat tgcgttaccc aatgtcccaa
    10681 cagtgcgccg ctgccgatac cgacgtt
//
LOCUS       DAXHRI010000056        10386 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXHRI010000056 DAXHRI010000000
VERSION     DAXHRI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.056"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(39..806)
                     /locus_tag="ACFM75_001070"
     CDS             complement(39..806)
                     /locus_tag="ACFM75_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC6255632.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALTALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVYETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(894..1721)
                     /gene="mutM"
                     /locus_tag="ACFM75_001071"
     CDS             complement(894..1721)
                     /gene="mutM"
                     /locus_tag="ACFM75_001071"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC6255633.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCTDYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1773..2438)
                     /locus_tag="ACFM75_001072"
     CDS             complement(1773..2438)
                     /locus_tag="ACFM75_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFC6255634.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFSCWFDGERLPEKRFCLPLPELKRRLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            2617..4593
                     /locus_tag="ACFM75_001073"
     CDS             2617..4593
                     /locus_tag="ACFM75_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6255635.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4661..5353
                     /locus_tag="ACFM75_001074"
     CDS             4661..5353
                     /locus_tag="ACFM75_001074"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC6255636.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAVGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
     gene            5899..7214
                     /locus_tag="ACFM75_001075"
                     /pseudo
     CDS             5899..7214
                     /locus_tag="ACFM75_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519747.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            7459..8115
                     /gene="cmk"
                     /locus_tag="ACFM75_001076"
     CDS             7459..8115
                     /gene="cmk"
                     /locus_tag="ACFM75_001076"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC6255637.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            8270..9955
                     /gene="rpsA"
                     /locus_tag="ACFM75_001077"
     CDS             8270..9955
                     /gene="rpsA"
                     /locus_tag="ACFM75_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC6255638.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            9966..10280
                     /locus_tag="ACFM75_001078"
     CDS             9966..10280
                     /locus_tag="ACFM75_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC6255639.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
BASE COUNT         2726 a         3027 c         2355 g         2278 t
ORIGIN      
        1 caaaagcgaa tcatactgcc acattccggc aaccggtttc agacagcgat atccgcatcg
       61 tcggcgacaa cggcacggat gctttcttcg attttatctt ttaaagcata acggtcttcg
      121 ctttccgccg catccgccac gcaaacgaaa tcgactttta tcgttaattt tttcatagac
      181 acaatgcgcc acaggcaggt cggcaaaccg acatcggcat atgagggacg agccgtcctt
      241 tttcccgttt cgtcataata acgcagcgcg accgccaaaa cctttgcccc cgcatcaatg
      301 gcggattgga acagcgcggc tttgaacggc aggagcccca gtccggagga agtccgcgct
      361 tcggggaaaa aactgacgtt ttgaccgcgt tgcaaggttt cgtagacggc gcggttaatc
      421 ggttcgatgt cgcgccgcga attgcggttg atgaacaccg ttcccgcgtt ctgccccatc
      481 ttgcccaata ccggccagct tttgatttcc tgcttggcga taaagctgct cggataaacc
      541 gcgctcatcg cgaaaatatc cagccaggac acgtggttgg cggcaaccaa gacaccgttc
      601 ggatgttcgg gtgcgggcct gcccacctcc aatccgatat ccaaagccgt cagagccccc
      661 ctgcccaact cgattaccgc ccgattgcgc gactcggggc agccgccgtc aataccgcgc
      721 aggtttttgc cggttttgaa tagccagacc gtcaagcggc acaagcggcg cagacgtgta
      781 aaaaatgaag ctttatttga agacatgaac aattctttcc cgataatcgg tgtttgttgt
      841 tgaaacgttc ggacggcata ccgataaaat gccgtctgaa accgttttcc gccctatttc
      901 tgacaattcg ggcagtaaaa cgtgccgcgc tgccccaaag tttctttcac aaccaaaccg
      961 ccgcactggg ggcacggctg attgtgccgc ccgtacaccg tatattcctg ttggaagtag
     1021 ccgcttttgc cgtcgctgtc cacaaaatcc ctcaaggtac tgccgcccgt ttcaatggcg
     1081 cgccgcaaca ccgctttgac ggtttcaacc aaaagcgcac actctttctt tttcagacgg
     1141 ttggcaggac ggtggggcga aatgcccgct ctgaacaggc tctcattggc ataaatattg
     1201 cccacaccga ccacgaccgc attgtccatc agggcaagtt tgaccgcgcg cttctgcgcc
     1261 ttcagcctca catacagata atccgtgcaa aatgcctccg acaaaggctc cggccccagt
     1321 ttttccaaca gcggatgatg ttcttcgatt ccctcatacc aaagtatcgc gccgaacttt
     1381 ctcggatcgc ggtaacgcat gaccgtgccg tctgaaaaca caatatcgac gtgatcgtgt
     1441 ctgtccggcc tgccgatacg tccgtccgac ggcgtaaaaa tccgcaagct gcccgacatc
     1501 cccaagtgaa tcagcagcac gcccgtttga aagcggataa gcaggtattt cgccctcctg
     1561 ccgcaggaca acacctgccg gccggacaaa atctccccca aatcgggatt aatctgccag
     1621 cgcagcttca attggcgcaa taccacggct tccaccgttt tcccttcaat atgcggcgcg
     1681 atgccgcgca acgtcgtttc cacttccggc aattcaggca taacccctcc cgacatttct
     1741 tctgacagat gccgtctgaa agacggctgt ccctaatccg caacccttgc cgcacccgcc
     1801 gcaagggctt tgccgcccaa atacccgtcc cacgcgggca ggggcgttac gcccgccgcc
     1861 cttttgacca aaaccaaacc gtacactgcg gcacactgcg gccaccaacg gtcgcccgcc
     1921 ttttccataa accgccaaaa gcgtatttgc ccgagcgacg aaaccggcgg cagatacacc
     1981 ataaatttcc caaattcaat atcgaaaccg acatccgcaa gccgtctttt caactcgggc
     2041 agcggcagac aaaaccgttt ttccggcagg cgttcgccgt caaaccaaca actgaatccc
     2101 cagagcgaat acggattgaa acccgtcagc atcaaacgtc cggacggttt caatatccgg
     2161 tgcgcttccg acaggatttg cgaaggaaca ccgccttcaa gcgtatgcgg aaaaagcagc
     2221 atatccgcag aaacatccgc caaagccata ttctccgccg acatcgacat atctcgcggc
     2281 acacagacaa catcttcaga caggctcagc cacctgccgc ccacctgaac cgcacacatt
     2341 cccgaaaaac ggtatgaatc cagataccgc ccgaagaaat cctgttccaa ttttgcaaca
     2401 taccgcccca tcgccgtatc ttcaaaccat gcatccatat tgcgtccgtt tcaaacagat
     2461 tgcctgccga tattctattc caaacagaat ttctgtcaaa aaacacatcg gccgcccact
     2521 tccgaatccg cataaagttc ctgtaaaact tgacgctttt tcagtcaaac agtaccatcg
     2581 gacgataaaa tatgtttatt ccgccgaata taaatcatgt ccaaactcaa aaccatcgct
     2641 ctgaccgcat caggtctgtc cgtttgtccg ggtttcctat acgcccaaaa cacctcatca
     2701 caccaaatcg gtttggcgat tatgcgctta aactcttcaa tactcgacct gcccccgaca
     2761 aaacaatatt tccaatccgg cagcctgtgg agcgagctgc gccaaggctt ccggatgggc
     2821 gaagtcaatc ccgaactggt acgccgccac gaaagcaaat tcatcgcaag ccacagctat
     2881 ttcaacaggg tcatcaaccg gagtagaccc tatatgtacc atatcgccaa cgaagtcaaa
     2941 aaacgcaata tgcccgccga agccgccctg cttcccttca tcgaaagcgc gttcgtcacc
     3001 aaagccaaat cacacgtcgg cgcatcagga ttatggcagt ttatgcccgc taccggcagg
     3061 cattacggcc tggaaaaaac accggtttac gacggcaggc acgacgttta cgccgccacc
     3121 gatgccgcac tcaactatct gcaatacctc tatggactgt tcggcgactg gccgcttgcc
     3181 tttgccgcct acaactgggg tgaaggcaac gtcggacgcg ccatcaaccg cgcccgcgcc
     3241 caagggctcg aaccgaccta cgaaaacctg cgtatgccca acgaaacgcg caactatgtc
     3301 cccaagctgc tcgccgtgcg caacattatt gccactcccc aatctttcgg catgaatatc
     3361 agcgacatag acaacaaacc ctattttcag gcagtcgaac cggatcgtcc gctcgacaac
     3421 gaagccatcg cccggcttgc cggcatcacg caaagcgagc tgctcgccct aaaccccgca
     3481 ttcaacgtcc ccgcgtttat ccccaaaagc aaacgcaaac tgctgcttcc tgtcgcatcc
     3541 gtacaaacct tccaaagcaa ctacctcaac gccgcacccg acagcctgtt ttcatgggaa
     3601 gtctatacgc ctgccgccaa aaccagcctg tccgacatct cgacggcaac cggcatgagc
     3661 attgccgaca tcaaacgcct caacaacctg aacggcaacc ttgtcaacgc aggacgcagc
     3721 atccttgtcg ccaagaacgg caaaaccctt cagacggcat cggaatccgt cgtttccatc
     3781 gacatcgaca atacgcccga cacctaccgt tccaatatgc cggcaggcac ggtgaacgtc
     3841 ggcattgccc gaatccgacc cgccgccgca cagacagcgg acattaccgt cgcacctttg
     3901 ccgcagaaaa ccgtccgtac ggaacccgat ccccttgtcc gtattgccga acctgccctt
     3961 gcgacagccg cagcgcaacc tcaaaccgaa aaacagaccg ccatgccgtc tgaaacccaa
     4021 accgcaacac tcgcgcagat catcccccaa aacgacatgc aggcggcaga cgaactcatg
     4081 cagcttgttg cccgaaacaa cctgcgccga caggctgaag aaaccatctc cgccgtcatc
     4141 ggcacgcctg acacagttgc cgaacacaac atttcctcat ctccgcaaca taccgttgcc
     4201 gccgacggca aacgccgggc acgtttggaa acgcgcgtag ccaaagctgc cgacggcgaa
     4261 gccgaaatct ccccgctcca tgccagcatc caccgcgttg tagaaggcga caccctgttc
     4321 aacattgcca aacgctacaa cgtcagcgta gccgacctga ttgtcgccaa caacatcaaa
     4381 ggcaacacca tccaaaaagg acaggtactc cgcctggcgc aggcagcccc tgcccaaacc
     4441 cgtattgaaa aagtatccta caccgcgcgc aaaggcgaca ccttcaaaag tatcgccgcg
     4501 cgcttcaata tccatatcga cgacatccgc cggctcaatc ccaacctgaa caccatcaat
     4561 ccgggacaga gggtcaaact gattggaagc tgattcggat acggcacatg acaggacttt
     4621 ctcagtcctg tctttttcat cccacatttc ccataccatc atgaaactca tcaaatacct
     4681 gcaatatcaa ggcataggaa gccgcaagca gtgccaatgg ctgattgccg gcggttatgt
     4741 tttcatcaac ggaacctgca tggacgacac cgatgcagac atcgattcct catctgtcga
     4801 aacgttggat attgacgggg aagcagtaac cgtcgttccc gaaccctatt tctacatcat
     4861 gctcaacaag cctgaagatt acgaaacttc gcacaaaccc aagcactacc gcagcgtatt
     4921 cagcctgttc cccgacaata tgcggaacat cgatatgcag gcggtcggca ggctggatgc
     4981 ggatacgacc ggcgtattgc tgattaccaa cgacggcaaa ctgaaccaca acctgacttc
     5041 gccgagcaga aaaattccca agctatacga agtaacgctc aaacacccca caggagaaac
     5101 gctctgcgaa accttgaaaa acggcgtgct gctccacgac gaaaacgaaa ccgtttgtgc
     5161 cgccgatgcc gttttggaaa acccgaccac cctgctgctg accattaccg aaggaaaata
     5221 ccaccaagtc aaacgcatga tcgccgccgt cggcaaccgc gtgcaacacc ttcatcgtcg
     5281 gcgattcgca catttggaaa cagaaaacct gaaacccggc gaatggaaat ttatcgattg
     5341 tccaaaattc tgaaacaaaa ttccataatt tccctatatt caactatatg cttattaaat
     5401 aagtagtttt acaaaaatta actcaaaaaa aaaaaaaaca gttagcttgc gatataattc
     5461 gcttgtctga gtaacgcctg ttcaagcagg cttatgagta agacgttttc cccgtaatgt
     5521 gttttgccat ctatacttct cccccttgta tagatggttt ttttatcttc gtttactaca
     5581 catactacat atgttactga cgcagcattc ccgatcatat gacatatgaa attttatttc
     5641 accgtcccag tgtagaaaaa taaacgattc atctatctat aaaatctgat tcaatccttg
     5701 ttgatttatg caaatcagaa atatccacta taaaatataa ctctattagt gaaaataact
     5761 acacacttaa aaaacaatca gtcagttcct gttaaaaaac gcgtgtagaa tttccgcttt
     5821 atctcaatcg gacacggttt caatatgtct tccccttcaa ataccaatcg tcaaacctga
     5881 tccagccgat taacctatat cctgaccgtt gccggcgcga ctgtcggttt cggcgcgacg
     5941 tggcgtttcc cgtatttggt cggtgaaaac ggtggtggcg cgtatgtgtt tttattctgt
     6001 atcgcgatgc tggttatcgg cattccgatg attttggtgg aaaatgtcat cggacggcgc
     6061 aaaggcgtga acgcgctgga tgcgttcggc ggtccgatga acggcaaacc cattgctaaa
     6121 atttggaaac tggtcggctg gatggggctg ctcggcgcgt tcggcatcat ggcttattac
     6181 atggcactcg gcggctgggt aatcagctat atcgttaata ttattggagg aaatttgaat
     6241 atttccagcc ccatcgacgg tgtggttaca aaaggcttct ttgccgaaca cattgaaaca
     6301 gcccttggga aattgcgttt tatacgctgc tttttgtcgc cgtgaaccaa tggattttgg
     6361 tcaaaggcgt tatcggcggc attgaaaaag cggcaaaata gctgatgccg ctgctgtttt
     6421 tgttcctaat cgcgatggtc gtccgcaacg ttacccttcc gggcgcaatg gaaggggttg
     6481 ctttctatct gaaacctgat ttcagcaaga ttaccgccga actgttcgtc ttcgttttgg
     6541 ggcaggtatt ttttgccctg agcctgggtt tcggcgtgat gattaccttg tccagctatt
     6601 tggataaaaa cgaaaatcta gttcagacgg cagttatcac ggcaattacc aataccatca
     6661 tcgccatact tgcgggcttt atgattttcc cgtcgctctt cagcttcggc gttgcccccg
     6721 attccggccc gactttggtg ttccaaagct tgccgattgt gttctcacat atgtgggcgg
     6781 gatctgtgtt cgccgtgatt ttcttctcgc tgctcctgat tgccgcgttg acaacttcgc
     6841 tgaccattta tgaagtgttg attacgacca ttcaggaaaa aaccaaaatc cgccgtaccg
     6901 ccgcgattac gattgtattg gctgccatct tcattttcgg caacatcccg tccattttga
     6961 gctacggccc ttggaaagat gtttccatat ttggcaaaaa tatttttgac gcttttgact
     7021 acatcagcgg caacatcttg tttatgctga ccgcgcttgg ctcggcattg tttgtcggtt
     7081 tcgtgatgaa ggatgaagcg aaggaagaat tgctttataa aggcaaccat acgacggtca
     7141 atatttggtt tgcctatgtg aaatacctcg tgccgctggt gattttgctg attttcgtca
     7201 gcaacctgtt ttaatccgca gcaatcgatg ccgtctgaag gtcatacctc tttcagacgg
     7261 cattgcattg atgtccgcca caatacccgc aacgccggaa tccgaaccgg cttgccggct
     7321 tctgtcggat atttccgggc aggcggcgtt ccgtctgctt agacaatcgt cctttaaaac
     7381 aggtagaatc cgcccccaac gggaaacaca tccttcagac ggcaaaaccc ataccccaaa
     7441 ccatcaggaa tcccccttat gaacagacaa aaagtcatcg ccatcgacgg tccgggcgca
     7501 tcgggcaaag gcacggtcgc cgcccgcgtt gccgccgcat tgggatacga ttatctcgat
     7561 accggcgcac tctaccgcct gaccgcccta tatgcacaaa aacaaggcgt ggaatggcac
     7621 gatgaagaaa acgtttccga actggcaaaa aaactgcccg ccgtattttc aggcagccgc
     7681 atcctgctcg gcggcgaaga cgtttcagac ggcatccgga cagaagccat cggcatgggc
     7741 gcatccgcag tcgcacagtt gcctaaagtc cgcgcctccc tgctgcaacg ccaacgcgat
     7801 tttctgaccg aaaaaggact ggtcgccgac ggacgggaca ccggatcggt cgtcttcccc
     7861 caggccgaac tcaaaatttt tctgacagcc gaatccaaaa tcagggccga acgccgcgcc
     7921 aaacaaatcg gtatcccctg cgaaggtttg gcattcgagc gcatcctgtc cgacatcgaa
     7981 gccagagacg aggcagaccg aaaccgcaag gttgcccccc tgaaacaaca acccgatgcc
     8041 ctgcttttgg atacgagccg cctgactata gaagaaactg taaaaaaagt gcttgattgg
     8101 tatcgtaaag tttaaatttt caggtataat cgcccgcatt gcgtttcaga cagcatgaat
     8161 ttccccatat gccgtctgaa accttattta cccgtctgcc ctgccaagga cggcagacat
     8221 ccaccaaccc accccgcacc ccttggcggt gtaccgaaaa gagttatata tgtctatgga
     8281 aaattttgct cagctgttgg aagaaagctt taccctgcaa gaaatgaacc caggtgaggt
     8341 gattaccgct gaagtagtgg caatcgacca aaacttcgtt accgtaaacg caggtctgaa
     8401 atcagaatcc ctgattgatg tagctgaatt caaaaacgct caaggcgaaa ttgaagttaa
     8461 agtcggcgac ttcgttaccg ttaccatcga atccgtcgaa aacggcttcg gcgaaaccaa
     8521 actgtcccgc gaaaaagcca aacgcgctgc cgattggatt gccctggaag aagccatgga
     8581 aaacggcaac atcctgtccg gcatcatcaa cggcaaagtc aaaggcggcc tgaccgttat
     8641 gattagcagc atccgcgcat tcctgccggg ttctttggtc gacgtacgtc ctgtaaaaga
     8701 cacttctcac ttcgaaggta aagaaattga attcaaagtg atcaaactgg acaaaaaacg
     8761 caacaacgtt gttgtgtccc gccgcgccgt tctggaagct accttgggtg aagaacgcaa
     8821 agccctgctg gaaaacctgc aagaaggctc cgtcatcaaa ggcatcgtta aaaacattac
     8881 cgattacggt gcattcgttg acttgggcgg catcgacggt ctgttgcaca tcaccgattt
     8941 ggcatggcgg cgcgtgaaac acccgagcga agtcttggaa gtcggtcagg aagttgaagc
     9001 caaagtattg aaattcgacc aagaaaaaca acgcgtttcc ttgggtatga aacaactggg
     9061 cgaagatcct tggagcggtc tgacccgccg ttatcctcaa ggcacccgcc tgttcggcaa
     9121 agtatccaac ctgaccgact acggcgcatt cgtcgaaatc gaacaaggca tcgaaggttt
     9181 ggtacacgtc tccgaaatgg actggaccaa caaaaacgta cacccgagca aagtcgtaca
     9241 actgggcgac gaagtcgaag tcatgatttt ggaaatcgac gaaggtcgcc gccgtatctc
     9301 tttgggtatg aaacaatgcc aagccaatcc ttgggaagaa tttgccgcca accacaacaa
     9361 aggcgacaaa atctccggcg cggttaaatc cattaccgat ttcggcgtat tcgtcggtct
     9421 gcccggcggc atcgacggtt tggttcacct gtccgacctg tcctggaccg aatccggcga
     9481 agaagccgta cgcaaataca aaaaaggcga agaagtcgaa gccgtcgtat tggcaatcga
     9541 cgtggaaaaa gaacgcatct ccttgggtat caaacaactg gaaggcgatc cgttcggcaa
     9601 cttcatcagc gtgaacgaca aaggttcttt ggttaaaggt tccgtgaaat ctgttgacgc
     9661 caaaggtgcc gttgttgccc tgtctgacga agtagaaggc tacctgcctg cttccgaatt
     9721 tgcagccgac cgcgttgaag acttgaccac caaactgaaa gaaggcgacg aagttgaagc
     9781 cgtcatcgtt accgttgacc gcaaaaaccg cagcatcaaa ctttccgtta aagccaaaga
     9841 tgccaaagaa agccgcgaag cactgaactc cgtcaatgcc gccgccaatg cgaatgccgg
     9901 caccaccagc ttgggcgacc tgctgaaagc caaactctcc ggcgaacaag aataaggttg
     9961 cagacatgac aaagtctgag ttaatggttc gtttggcaga agtgtttgcc gccaaaaacg
    10021 gcacgcatct tctggcaaaa gacgtagagt acagcgtaaa agtcttggtt gacaccatga
    10081 ctagatcgct tgcccgaggt caacgcatcg aaatccgcgg tttcggcagc ttcgatttga
    10141 accatcgtcc tgcccgcatc ggtcgcaatc ccaaaaccgg cgagcgtgtg gaagtacctg
    10201 aaaaacatgt accccacttc aagcccggta aagaattgcg cgagcgggtc gacttggctt
    10261 taaaagaaaa tgccaattaa accttagcat caaaacgccg ctgttacgcg gcgttttttc
    10321 tgtggtttaa cttcatccgc tgcttcaata ccttgagcca agcaagcaac ggattagagc
    10381 gtggat
//
LOCUS       DAXHRI010000057        10205 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXHRI010000057 DAXHRI010000000
VERSION     DAXHRI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.057"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(45..121)
                     /locus_tag="ACFM75_001079"
     tRNA            complement(45..121)
                     /locus_tag="ACFM75_001079"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Arg,seq:acg)
     gene            complement(231..1904)
                     /gene="recN"
                     /locus_tag="ACFM75_001080"
     CDS             complement(231..1904)
                     /gene="recN"
                     /locus_tag="ACFM75_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC6255640.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2088..2690)
                     /locus_tag="ACFM75_001081"
     CDS             complement(2088..2690)
                     /locus_tag="ACFM75_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC6255641.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPEAVPSDTLPN"
     gene            complement(2791..3732)
                     /gene="ylqF"
                     /locus_tag="ACFM75_001082"
     CDS             complement(2791..3732)
                     /gene="ylqF"
                     /locus_tag="ACFM75_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC6255642.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3813..4667)
                     /gene="rapZ"
                     /locus_tag="ACFM75_001083"
     CDS             complement(3813..4667)
                     /gene="rapZ"
                     /locus_tag="ACFM75_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC6255643.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4648..5610)
                     /gene="hprK"
                     /locus_tag="ACFM75_001084"
     CDS             complement(4648..5610)
                     /gene="hprK"
                     /locus_tag="ACFM75_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC6255644.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNENRSD"
     gene            complement(5612..6061)
                     /gene="ptsN"
                     /locus_tag="ACFM75_001085"
     CDS             complement(5612..6061)
                     /gene="ptsN"
                     /locus_tag="ACFM75_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC6255645.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6246..7136)
                     /gene="ubiA"
                     /locus_tag="ACFM75_001086"
     CDS             complement(6246..7136)
                     /gene="ubiA"
                     /locus_tag="ACFM75_001086"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC6255646.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            complement(7166..7642)
                     /locus_tag="ACFM75_001087"
     CDS             complement(7166..7642)
                     /locus_tag="ACFM75_001087"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC6255647.1"
                     /translation="MEHLFGKWLPDLPADVSDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7657..8304)
                     /gene="bioD"
                     /locus_tag="ACFM75_001088"
     CDS             complement(7657..8304)
                     /gene="bioD"
                     /locus_tag="ACFM75_001088"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC6255648.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGIPMQEADKRRLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8301..9602)
                     /gene="bioA"
                     /locus_tag="ACFM75_001089"
     CDS             complement(8301..9602)
                     /gene="bioA"
                     /locus_tag="ACFM75_001089"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC6255649.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDDLLTELAGKTVHILKEYSK"
     gene            9818..9933
                     /locus_tag="ACFM75_001090"
                     /pseudo
     CDS             9818..9933
                     /locus_tag="ACFM75_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(9931..10158)
                     /locus_tag="ACFM75_001091"
     CDS             complement(9931..10158)
                     /locus_tag="ACFM75_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255650.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVL"
BASE COUNT         2370 a         2789 c         2574 g         2472 t
ORIGIN      
        1 atgtcctaac cgctagacga tgggcgcata ttgtattttc ttactggcgc acccggagcg
       61 attcgaacgc ccgaccctct ggttcgtagc cagatactct atccaactga gctacgggtg
      121 cagcacaatc acttgttcaa gtgattaaga aagcgtgata atacggattt atctgagttc
      181 tgtctattgt tttagacttt tatttttagt tattttttaa ctgattgatt ttaaaataaa
      241 ctatttttcg acgcaagttg cagcaattct gccgcatgtt gccgcgtcgt atcggtaatg
      301 acttctccgc ccaacatacg ggcaacctct tcgatccgtt ggatttcatc caatatactg
      361 atttcgctga cggtttgctc tccctcgctg tgcttgcgca cccgccagtg gttttctccg
      421 caggatgcga cttggggaag gtgggtaacg gcaagcacct gatgttttct gcccaacgca
      481 cgtaatgcct tgccgaccat ttcagccact ccccctccaa taccggtatc gacctcatca
      541 aaaatcaggg tgggaacttg ggtatattgg ctggcaacaa cctgtaaggc aaggctgata
      601 cgcgccaatt cgccgccgga ggcaacttta ttcagcggac ggggcggatt gcctttgttg
      661 gcggcaactt gaaattgaac ctgctccaaa ccgtgtgccg tcggcgacga gggcaacagg
      721 acgatgtcga aacgtgcacc tttcatggca aggtgttgca tatgctcggt cgtttcgccg
      781 ctcaaacgct ctgccgcctg atggcgcatg gcagaaagga tgtgggcagc ttcctgatat
      841 tcggcaaaat tgtgggcaac attatgctcg agcgcgtcca aatcggcggc agcttgcagg
      901 ctttgcaggc gttcttcgat ttctgccaac ttggcaggca actcttcagg ctcgatccgg
      961 tatttccgcg ccatccccat cagttcgccc atacgctgtt cctgtgccgc caattcattg
     1021 gggttgatgt cgctgcgacc tgccacatcg cgcatattgg cactgatttc gcccaattcg
     1081 gcctcgatgc ttgccaacat attcaggctc tcggcaaagc gcggctcgat gttttgcaga
     1141 ttggccaata gtttttgaca ttgatagata tggcgttgga tgccgttgtc gccgtcaatc
     1201 ttgcttccga cttcttcggc agcctgcaac agctcggcag aatgggcaag gctgtcgtgg
     1261 ctttggctga gggcttccca ttcgccttgt ttaatgtcca actgattcaa ttcgttaaac
     1321 tgccattcca gacgctcttg ttcgagtgca atgttttctg cctgttcctg cgcttcctgc
     1381 aatgcttttt ttgagtttgc ccagttttga taaagctgcc tgacggtttc cgcctgcgcc
     1441 ctgctgcccg caaatgcgtc caacaattcg cgctgagcgg cttcctgatt aagcgaatga
     1501 tgggcgtttt gcccgtggat gtcgataagc tggctgccga cggctttgag ttgcgccaag
     1561 gtagcggcct gattgttgat aaagctgcgg cttttgcctt tggcatcgat aatgcggcgg
     1621 atactgagtt cttcttctcc gtcgtttaaa agcccctgtt catacaattc tgcttttaaa
     1681 acaggtaaat gggaaatatc aaacaacgcc gacaactgcg cttcttttgc gccgctgcgg
     1741 acttggctgt aatcggcttt atcgcccaac agcagaccaa tcgcatccaa agtaatggac
     1801 ttgcccgcgc cagtttctcc ggtcaatacg gtaaagccgc tttgaaaatc cagattcaga
     1861 ttttcgacaa tgacaaaatc acgcaaagaa agtgttagaa gcatcgtgag gattccttag
     1921 caattctacc gacaaaagca atatcaactt tagatatacg aattataact aagatttttt
     1981 aaattaaaaa taagtttttc cttaattttg cttagtgctt gtttttatct gcttatttgt
     2041 tgaaatgccg tctgaagcaa tgtgcgtttc agacggcatt tggaatttca gttgggcagg
     2101 gtatcggacg gtacggcttc cggctcttct tcatccgtag gcggcatttc tatcaaatcg
     2161 ggtaaaacca gttcgcccag ttcggtcagc ggcggaagtt cttccagacc gtccaagcgc
     2221 aaatcgctga gaaaagttgc cgttgtcgcc cacaacgcgg gttttcccaa tgtgtcccga
     2281 tgtccgatga cttcaatcca cccccgatcc tgcaaggtct gcatcacgtt ctgcgacacc
     2341 gccacgccgc gtataccttc gatgtcgccg cgcgttacgg gctgctggta ggcgataatc
     2401 gccagtgttt ccatcacggc gcgggagtag cgcggcgcac gctgttcttg caggctgccc
     2461 agccgctcga atgccgtctg aacaatctga aaacgccagc cctcttgcgt atgcaccagt
     2521 tgcaacgccc tatcctgcca acgcgttttc aactgcgcca acacatcaat cagtttgtcc
     2581 tgcgacagtg gcggcacaca caattcgcgc atagattttt cggtcagcgg ttcggtttgg
     2641 gtcagcagtg cggcttcaat tagtgcgtcg ggagaaattt tgtcggtcat acgggtatcc
     2701 gtgctgaaac ggcatgggtt tggatatgcc gtctgaaatc ggttggagta gagagaagct
     2761 gcctgaaaaa tatttttcag acggcattct ttatgcttcc gaaggcttct gccctttacg
     2821 ctctgctttt ctggcttcgc gtatggcttt gagttcggct tctttctgac gggctttttt
     2881 gagccaagtt tcccattggt tcggcgtttc gagggtgatt ctgccgattt tgccttcacg
     2941 gaagtcggtg aggatgtttt cggcggcttt ttggtagttg atccgtccgc cgctgaggac
     3001 tgcgccgcgt tttttggcta tccattcgag ccaaacgttt tcgtcccagt ggctgctggg
     3061 gtctttgtcg gcttggtagc gttcttgcaa catagggagg tagtggcggc ggaggtagtc
     3121 taaaagttcg agggcgactt cttcttcgtc caatgcgttg cgtccgactg aaccgccggc
     3181 ggcgaggttg tagccgcttt cttcgacaat gattttcggc cacagcattc cgggggtgtc
     3241 gtacagccag aagtcgtcgg caaggaaaag gcgttgttcg gctttggtga tgccgggttc
     3301 gttgccggtt ttggcggatt ttttgcctat catgccgttg atgagggtgg atttgccgac
     3361 gttgggaatg ccgcaaatca aaacgcgcag gggtttgtct atgccttgcc ggtggggaat
     3421 catcgcgcgg caggcttggg tgattttgcc gtgtgcgccg gtttcggagg aatcgagggc
     3481 gatggcgcgc gtgtcggggc ggctgttgta gtggtcaagc cagacctttg tgcgttcggg
     3541 gtcggcaagg tcttgtttgt ttaaaatttt gagtttgggt ttgcctttgg aaagctgtgc
     3601 aagcaggggg ttttcgctgg aggcgggcat acgcgcgtcc agcatttcaa tcaccatatc
     3661 aacgcttttt gcacgctcgg cgatggcttt tttcgccttg ttcatatggc cgggaaacca
     3721 ttggattgcc atgtctgttc tttcttttca atatttgaaa tgccgtctga aacggaggac
     3781 ggggtttcag acggcataat ggtttgacgg aattagcggt ctgacaggtt ttgcgcctgt
     3841 ctgtgccgta tcagcaattc ataacgccct ttcaaccttc gggcgagttt ttcgacaata
     3901 tagaccgaac ggtgctgtcc tcccgtgcaa ccgatggcga cggtaacgta gctcctgctt
     3961 tcatcctcca aacgcggtaa ccaatgcgta acaaaccttt cgatgtcgtc aaccatttcc
     4021 tgcacaagcg gctgtccgtc caaataatcc caaacgggct tgtccatacc ggtgtaaggc
     4081 ctcaactcgg gatcgtaata cgggttgggc agactgcgca tatcgaacat aaaatccgcg
     4141 ttgttcggca caccgtattt gaacccgaag gactccaaaa tcaccagcag cccggtacgt
     4201 tcgaccttca gccactgccg gactgcatgg cggagctgtt gggcattcat cttggaagtg
     4261 tcgatacaat aggcgatttc tttaagcggg aacagccatt cccgttcttt ctttaagctt
     4321 tccaacaagg tcatatcctg attactcaga gggtggcctc gcctggtttc ggaaaaccgg
     4381 cggaccaaca cgctttcttc cgcctcgaca aacaaaactt caaccctgtg ccccagtccg
     4441 cgcagagagg caatctgttc ccgcgcctgt ccgatgtcta tgccggaacg cacatcgacg
     4501 ctgaccgcca attcggtttc gtccgcacgt tcgatatgat acgacaccag cgcgggcaac
     4561 atttccaaag gcaaattgtc cacgcagaaa taacccgaat cttccatttg gcgcagtgcg
     4621 acggatttgc ccgaaccgga caacccgcta atcagaacga ttttcattgt gttgctcgtt
     4681 ttctttaagc tgcgtctgat gacgttccaa aaattcgcgc gtactatcct taccgcgcaa
     4741 ctgaagaata taattgcgta ccgccgcctc aaccaaaacg gcgaggttgc gtccgacggc
     4801 gacgggcagc gtaaccgaac ggacgttgac gttgaggatg gattcggttt cggtgcggat
     4861 gctcaaacgg tcgagctgct tcatatactc gtcgtccgcc tcgaccaaat tgataatgag
     4921 ttgcaggatt tttttggggc ggatggaagt ttcgccgaaa atatggcgga tattgagtat
     4981 ccccaagccg cgtacttcca aaaaatcgcg cagcataggc gaacaccgcc cttccagcgt
     5041 ttccgggccg atgcggaaca gctcgaccgc atcgtcggca atcaggctgt ggccgcgcga
     5101 aatcagttcc aatgccaatt cgctcttacc caaacccgaa tgtccggtaa tcagcacgcc
     5161 gatttcaaac acatcgagaa atacgccgtg tttgacggac gatgccgcca gggtgcgttg
     5221 caggtaaatc cgcaacacat ccatcagata ggggctttcg agtttggaag tcagcagtgg
     5281 aatatcgttt ttatggcagt agtcgcgcag tcccggggaa accggcaagc cgtttgccac
     5341 aatgaccaaa gacatagaaa tgtcgaacag gtcgccgaac tgataacccg tttcccccga
     5401 ttcgagacgg ttcagatatt ccgactccgc caaaccgacc acttggattt ggttggggtg
     5461 gataaaattc aggtgtccga ccaaggcgag gacgggcttg tccgcctcta cgccgatacg
     5521 gttgtccgca cccgaattgc cagcggccca ggcaagttgc agtttgtatt ggttgtcgtc
     5581 aaacagccgg cggacagaga tactgggcat attattcttc agtcaggatg gcgcgggctt
     5641 cttccgcaga agaaacggtc atcagcgatt ctctgatgct tttttgggaa aacttgccgg
     5701 ccagtttgga caatacttcc aaatgctcac ccgtcgcatt ttccggaacc agcaagataa
     5761 aaaccaggga aaccggtttg ccgtccggtg cgtcaaatcc gacgggttcg cgcgtgcgga
     5821 tgaacgcgcc cgtcgcctgc ttcacgccgg catgacggcc gtgcgggatg gcaacgccct
     5881 gccccaaacc ggtcgaaccg agtttttcac gggcaaaaag acattcgaaa acatcagcat
     5941 gggacaatga ggattcgcgt tccaaaagca ggcccgcttc ctcaaacagc ctttttttac
     6001 tgcccacctc catatccaaa acaatatggg acaaaggcaa aatttcgccg ataaggctca
     6061 taagcttctc ttttcagaca tcgcaaaaca gaaagattgt accgactgcc agggcaaatc
     6121 tcaatcccgc atacggtatg ggctgacata acacagcgtt ttaaaaacat attttaacgc
     6181 ttttcggcac agatagaaat gccgtccaaa gcagtttacg gctcttcaga cggcattgcc
     6241 ctgccttatt tcgcgaaaaa cgtatgggca aaaatggcgg caaaccacac ccaaccaatt
     6301 ctgttgtttg ccaaaaacgt ttcaaaacag atttgccgga cgcggctttt gatggcggca
     6361 tattggcggt attgcagcag caggacgatg gggattgccg tccaatatgc ccatgccgca
     6421 ccgataaccg cacccaatac tgccatcagc agggtaaagc ctccgtgaca cagcataacg
     6481 gcggcgatgt cgtaacgccc gaacgtgacg gcggaggttt tgatgccgat tttcaaatcg
     6541 tcctctttgt ccgccatggc gtaaaccgtg tcatacgcca gagtccataa cacattggcg
     6601 gcaaagagta tccacgcttg aggcggcacg tttccggcaa cggcggcaaa cgccatcggg
     6661 ataccgaagg aaaaggcaag cccgagatag agttggggaa tcggaaaaaa acgtttggta
     6721 aacgggtaag tcagcgcaag aaacagcgcg ggcaggctca tcagccaagt cagatgattc
     6781 agcggaatca ggcacaatgc ggcaagcagg cacaaaaatg ccgtcagcag cagcgcttct
     6841 tttttcttga ccctgccctg tgcgaacgga cggtttttag tgcgctcgac agccccgtca
     6901 aaatcgcggt cggcaaaatc gttgatgacg cagccggcac tgcgcattaa aaacgtaccg
     6961 attgtaaacg ccgccaatac cgccaaatcg ggaatgccgt ctgaagccag ccacaatgcc
     7021 cagtaggtcg gccacagtaa aagcagcgtc ccaatgggct tgtccgcccg catcaggcgc
     7081 aggtacacat ccaaacggtc ggacagtcgt aaaaataaag gggatttagg attcatattg
     7141 ccgcacagct tgaaaaaacg gtattttatc cgataaagcg tttcagttcg ggcagaaaat
     7201 actcggtcag cagcatttcc tcgccgtgac gggaaaaccg agaacgccgc gcggcaaagt
     7261 accgtccgca tccttcgccg gaaacggcaa actcaaacgc cgaacgcgcc ccttccaaat
     7321 cggcttgaaa cagacgctcg cccaaagggc gcgtgccgca gtccaaaatg ttttgccaaa
     7381 acgccgaacc tatccggcac tcgctccttg ccgcgacaac agggataccg tccagcttca
     7441 acaaaacttc gcgcaccagc ctccctccgc attccgtctc caattcgccc agtttcagca
     7501 gttccaccga aaatgtatgc ggcaaggcgc gcaatgcggc ggtcagcgac cgggtgtgca
     7561 gcagccgcac catcggcagg ctgatgccgt ctgaaacgtc ggcgggcagg tcgggcagcc
     7621 atttcccaaa taggtgttcc atatttttcc caatctttat accgcgtctg tttttgccaa
     7681 ctccatccat tccgcttcgg aaaaatcggc tttcagacgg catttcaagt agttcaggct
     7741 gtcttgggcg atgtgtgcgt cgcggctgtc gtggatgtgg ttgaacacga ggctgtacaa
     7801 gcgaatgccg tattgtttga gcgcggcgaa actgagtaag gtgtggttga tgctgcccag
     7861 ccgtccgctc gttacgagaa tgacgggata gccttgctga cggatataat caatggttaa
     7921 cagcttttcc gtcagcggaa ccatcaatcc gcccgcgcct tcgaccaaaa cgacttcgta
     7981 ctgcgccgcc aattcttgtg tggcggtgcg gattttgtcc aagtccaaag ccctgccgtc
     8041 cagtcgggca gcgagatgag gcgaagctgg ataactgaag atttcaggca tggtcagccg
     8101 ccgtttgtcg gcttcctgca tcggtatgcc cataatttta cgatggacgg cgatgtcgtc
     8161 ggcaatgttt tggcaaccgg tttgcacggg cttttgcgta atcacgcttt tgccctgctg
     8221 caacaattgt tttgccaata cgccggtggc gacggttttg ccgatgtccg tatcaatgcc
     8281 gctgacgaag taaacgcctt tcatttgctg tattccttca aaatgtgtac ggttttgccg
     8341 gcaagttctg tcaagaggtc gtctgaaata atgtaaggcg gcatgaggta caccagcctg
     8401 ccgaatgggc gcacccaaat gccctgcgcc acgcaatccg cttgaaaacg cgccatatcc
     8461 acgccttttt ccaactcgat cacgccgatg gcgcccaaaa cgcgcacgtc tttcacacca
     8521 cgaatgtccc acgcggtttt cagacggcat tttaagatgc tttcgatgcg gcggatattt
     8581 gcctgccaat cttgggacaa aagcagtttg accgaagcgc aggcaacggc acacgccagc
     8641 gggttcgcca taaatgtcgg gccgtgcatg aacacgcccg cttctccgcg cgaaatcgtt
     8701 tcggtaactt tttgcgaagt gattgctgcc gccagcgtca tatagccgcc gctcaaaccc
     8761 ttgccgatgc acataatatc cggcacgacc tccgcgtgtt cgcaggcaaa catcttgccc
     8821 gtgcgcccga atccggtggc gatttcatca aaaatcaaaa cgatatcaaa ctcgtcgcac
     8881 aaatcgcgca atccgcgaag atactgcgga tgataaaaat acataccgcc cgcgccctgc
     8941 acgaccggtt ccaaaataaa ggcggcaatg tccgtatgat gcgcttcaaa caaggcgcgg
     9001 acaggctgca aatccgcctc gtcccattca tcgtcgaaac ggcttgtcag gttatcgaca
     9061 aaataacgct gcggcaacgc gctgccgaaa atatggtgca tccccgtttc cggatcgcag
     9121 acggacatcg cgttccaagt atcgccgtga tacccgcgcc gcaccgtcgc catattttgt
     9181 tttgcacgca aaccccgcgc ctgctggtat tgcaccgcca tcttcaacgc gacttccacc
     9241 gaaaccgaac ccgaatccgc ataaaaaata cgatccagcc cctgcggcaa aatcccgacc
     9301 aacaatttgc ccagctccac cgccggctca tgcgtcaaac cgccgaacat cacatgcgac
     9361 atctgtttca tctgcgtctc aaccgcctga ttcaaaacgg gatgattgta accgtgtatc
     9421 gcacaccacc aagacgacat cccgtcaatc agccgcgtgc cgtccgccaa ttcgataaac
     9481 accccttctg cacgtttgac aggataaacg ggaagcggat cggtcatgga agtataggga
     9541 tgaagcagat gggtacggtc gaaatcaagc aatgatgatg tatgttgatg ttcagacggc
     9601 ataagtttct ctcttttctt cttactgtat tcaaacgcaa acgcgtattc tactccgaca
     9661 gaccgcttcc cacacctttc catccgtttc gggcgcaaaa ccgcgaaaca aatcgtccgc
     9721 agcataagcg cacaccgttt cgcattcctc aagcctgatt ggaatcagac gacccaacgc
     9781 ccaataccgt ttcacacagc ctccgcccaa cgcttcaatc agtcatagat atagtggatt
     9841 aacaaaaatc aggacaagga gacgaagccg cagacagtac aaatagtacg gcaaggcgag
     9901 gtaacgccgt actggtttaa atttaatcca ctataaaacg gcaatccata cgatacagat
     9961 catagcaaca gccatcgcaa cagcgttggc aaaatcaggg gactccgaca ttggcgcata
    10021 gcatctgccg atgcacggct cctcattcgg ctctatgaat accataccca tcacaaaatc
    10081 caccgccaaa accaggcacg gcttcttata cttatgatag atttccacca tcctgcccca
    10141 tatatcccaa acattcatac cgcatatccc gcaggcaaca aattccgatt gaaggttaca
    10201 gccct
//
LOCUS       DAXHRI010000058        10195 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXHRI010000058 DAXHRI010000000
VERSION     DAXHRI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.058"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..1883)
                     /gene="tspA"
                     /locus_tag="ACFM75_001092"
     CDS             complement(<1..1883)
                     /gene="tspA"
                     /locus_tag="ACFM75_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC6255651.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKDSSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENVTETV
                     AETP"
     gene            2191..2721
                     /locus_tag="ACFM75_001093"
     CDS             2191..2721
                     /locus_tag="ACFM75_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC6255652.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            2724..3014
                     /locus_tag="ACFM75_001094"
     CDS             2724..3014
                     /locus_tag="ACFM75_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC6255653.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            3014..3292
                     /locus_tag="ACFM75_001095"
     CDS             3014..3292
                     /locus_tag="ACFM75_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6255654.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAG"
     gene            3341..4207
                     /locus_tag="ACFM75_001096"
     CDS             3341..4207
                     /locus_tag="ACFM75_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6255655.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            4278..5036
                     /locus_tag="ACFM75_001097"
                     /pseudo
     CDS             4278..5036
                     /locus_tag="ACFM75_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
     gene            5110..5601
                     /gene="dtd"
                     /locus_tag="ACFM75_001098"
     CDS             5110..5601
                     /gene="dtd"
                     /locus_tag="ACFM75_001098"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC6255656.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            5632..6642
                     /gene="dusA"
                     /locus_tag="ACFM75_001099"
     CDS             5632..6642
                     /gene="dusA"
                     /locus_tag="ACFM75_001099"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC6255657.1"
                     /translation="MNDNTHTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGITVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNHAPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSNATLLKDNDGGLILEAWEEVERANT
                     RE"
     gene            complement(6710..7597)
                     /gene="gluQRS"
                     /locus_tag="ACFM75_001100"
     CDS             complement(6710..7597)
                     /gene="gluQRS"
                     /locus_tag="ACFM75_001100"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC6255658.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSHRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPAPQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(7614..7904)
                     /locus_tag="ACFM75_001101"
     CDS             complement(7614..7904)
                     /locus_tag="ACFM75_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255659.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            complement(7909..8043)
                     /locus_tag="ACFM75_001102"
     CDS             complement(7909..8043)
                     /locus_tag="ACFM75_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255660.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGFALKILINKKLMHDITHQHKRK
                     "
     gene            complement(8058..9113)
                     /gene="tal"
                     /locus_tag="ACFM75_001103"
     CDS             complement(8058..9113)
                     /gene="tal"
                     /locus_tag="ACFM75_001103"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC6255661.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSIAHIQVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETKGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            9210..10184
                     /locus_tag="ACFM75_001104"
     CDS             9210..10184
                     /locus_tag="ACFM75_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC6255662.1"
                     /translation="MAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQCRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTQP
                     KTISTERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
BASE COUNT         2180 a         2560 c         3026 g         2429 t
ORIGIN      
        1 ggtgtttcgg caacggtttc cgttacattt tcctcaacgg acggtgcttc ggcaggttga
       61 agcaatgcgg acaaatcgtc tgcggcggcg ttgaaatcgg gcgtttcagg cgcagtttcc
      121 gcgacggcat cggtttcgta cactttcagg aaatcgtgca actcttccgg tgtttggact
      181 tcggcaactg ttttttccaa gatggtttcg ggcgaggaat ccttcaggaa gcctgccagt
      241 ccggagggtg aggcaggttt tgcggaagct gtttcttctg tgccgatatg gttgtttgag
      301 ggcaggttgt cggagaaatc ggtatcgacg gtttccggtt tgttttcggc agtttgggcg
      361 acagattccg gttcgggcgt gtcgatgacg atttcgacag ggttgtacgg gttgaaggtc
      421 tcgggctcgt acacgctgtc tgtggattcg atggcgttcc aatcggcatc cgcgcgtttt
      481 tgggtttctt catcctgcgt aagtgcgccg gataaaatgc cgttttgcgc ggctgccagg
      541 ctgtcgaaat ccaagtcgat gcggttggaa ggcgtatcgg tttcgacatc gaacgtttgt
      601 tttgccgata actcttcttc agattcccca tctaaggcaa gtgtgtcgtt tacatcgttt
      661 ttcggagcgg gttcgggcgt tgccggagtt tcgacttcgg caaaggtgat ttctatgccg
      721 tcgtctgccg cgtcgtcaag gtcaggctct tcctcaggga cggattcttc ggtacggcgc
      781 gcgcgtttgg attgggcaag gcgcaaaagc agcagcaggg cgatcaatgc cgcgcctccg
      841 ccggcaagca gcaaggtgta cgaaccgccg aacagaccgt caaacagtcc gctttcggtt
      901 tcttcttcgg cagaaacctg ttcgacaggt tcggaaacgg cgttaccggt ttcgtcggtc
      961 ggcgtgtcga tggcagaagc ggcggcttct tggggggcgg attcggcagc ggtttccgat
     1021 gcggcagtat ttgcagcggg tacaggttcg ggtcgaacgg ccggtttttc cgcttttgct
     1081 tcgggcgcgg caacttttgc ttcaggtttt tcaaccggtt tctctaccgt tgcctgtttg
     1141 gacggttcgg acggcatgga tgcggtttcg gctttgggtt tcgccgtttg cggtttgggt
     1201 tgttccgctt tgatcctgtt cagattcgga atgtgaagca cgctgcccgc acgcagtctg
     1261 ccgtgtgcgg aaacatttgg gtttgccttc agcagcgcat cggcaacctg ttcgagcgtc
     1321 aggtgtttcg gacggatggc ggcggcaatc tgtttgaccg tttcgccttt gcggacggta
     1381 tgggttttgc cgttgtacgc cggtttgacg gctgcgttcg cgctgtcttt tttatcggtt
     1441 ttgcggaggg ctttggcgtt ttgattttct tgggactctg ctgtcggagc ggttttgcgg
     1501 tgtgtcttgc cgtctgaaag tgcagatttg gttttgggcg agtagccgac aggatcgagg
     1561 atggcggtgt attcgcgtac ctgtgcgcct gcgccgatgc ggaataccag gacgggatcg
     1621 cggactgcct gttcggaaga aacggcaatg acggctttgt cgcccaactt gtggactttg
     1681 gcggtcaggc ctttttcgga aacggtaacg ctgccgccgc ctagcagggc tttggcttct
     1741 tcgccggtta cggtaatgct gccggaaaag ggttcgtcaa ggttggactg gatattcagt
     1801 ccgcccagtc cagcatgtgc ctgaaaggat gcggcaactg cgacggaggc ggcaatcagt
     1861 ttgatttgtc tgttgttttt caagatgtat cccctgtggg ttggcggctg aatacggttt
     1921 gaccgcgtac agtctgtaaa tttcgtcatc atcgggcatc ggcggggcag tcggccggcg
     1981 ggcatttaat atgtgaatgt accgaccgcc gccacatttt aaacggcaat cattcgccgt
     2041 ttttacaaat tatgacatat ctccatcttt tttcaaaaac atctgtgcat atttgcatca
     2101 atcaaaacaa aatttgttgg ttttgcaggt gcaaaaacag ggttctgcct gtatgattag
     2161 cgtttatttg atttgctttc tcatttggat atgaaattcg tcagcgacct tttgtctgtc
     2221 atcttgtttt ttgctactta taccgttacc aaaaatatga ttgccgctac ggcggttgcc
     2281 ttggttgccg gtgtggttca ggcggctttt ctgtattgga aatataaaaa gctggatacg
     2341 atgcagtggg tcggattggt gctgattgtc gtattcggcg gcgcaaccat tgttttgggc
     2401 gacagccgct tcattatgtg gaagccgagc gttttgtttt ggctgggcgc gctgttcctg
     2461 tggggcagcc acctcgccgg taaaaacggc ttgaaggcga gtatcggcag ggagattcag
     2521 cttccggatg ccgtatgggc gaaattgacg tatatgtggg tcggtttcct gatttttatg
     2581 ggtatcgcca actggtttgt gtttacccgg ttcgagtcgc aatgggtcaa ctataaaatg
     2641 ttcggctcga ctgcactgat gcttgttttc tttattattc agggtattta tctgagtacc
     2701 tgtctgaaaa aggaggattg actgtggaat attttatgtt gctggcaaca gacggggaga
     2761 atgtgtatga agcgcgtatg gcggcacgtc ccgaacacct caaacggctg gagacgctga
     2821 agtcggaagg ccggctgttg acggcaggcc cgaatccttt gccggaggac tccaaccgcg
     2881 tttcgggcag tttgattgtg gcgcagttcg agtctttgga cgcggcgcag gcttgggcgg
     2941 aagacgatcc ctatgttcat gcaggcgtgt acagcgaagt gctgatcaag ccgtttaaag
     3001 cggtgttcaa ataatgccgg ccgtcgattt gatccgcgaa cgcctgcaga cgctcgatcc
     3061 gctggtgttg gaaatcggcg atgagagcca tctgcacaaa ggacacgcgg gcaataccgg
     3121 cggcggacat tatgccgttt tggtcgttag cggccgtttt gaaggcgtaa gccgcctgaa
     3181 ccgccagaaa acggtcaaat cgctgctcaa agatttgttt tcaggcggca tgattcacgc
     3241 gctcggcatc cgggcggcta cccctgacga gtatttccat acggcgggct gaatgaagtc
     3301 tgcccgaaca tttcaattta aaatttaaag agagaagatt atgaaagcaa aaatcctgac
     3361 ttccgttgca ctgcttgcct gttccggcag cctgtttgcc caaacgctgg caaccgtcaa
     3421 cggtcagaaa atcgacagtt ccgtcatcga tgcgcaggtt gccgcattcc gtgcggaaaa
     3481 cagccgtgcc gaagacacgc cgcaactgcg ccaatccctg ctggaaaacg aagtggtcaa
     3541 caccgtggtc gcacaggaag tgaaacgcct gaaactcgac cggtcggcag agtttaaaaa
     3601 tgcgcttgcc aaattgcgtg ccgaagcgaa aaagtcgggc gacgacaaga aaccgtcctt
     3661 caaaaccgtt tggcaggcgg taaaatatgg cttgaacggc gaggcatacg cattgcatat
     3721 cgccaaaacc caaccggttt ccgagcagga agtaaaagcc gcatatgaca atatcagcgg
     3781 tttttacaaa ggtacgcagg aagtccagtt gggcgaaatc ctgaccgaca aggaagaaaa
     3841 tgcaaaaaaa gcggttgccg acttgaaggc gaaaaaaggt ttcgatgccg tcttgaaaca
     3901 atattccctc aacgaccgta ccaaacagac cggtgcgccg gtcggatatg tgccgctgaa
     3961 agatttggaa cagggtgttc cgccgcttta tcaggcaatt aaggacttga aaaaaggcga
     4021 atttacggca acgccgctga aaaacggcga tttctacggc gtttattatg tcaacgacag
     4081 ccgcgaggta aaagtgcctt cttttgatga aatgaaagga cagattgccg gcaaccttca
     4141 ggcggaacgg attgaccgtg ccgtcggtgc actgttgggc aaggcaaaca tcaaacctgc
     4201 aaaataattc tgaaaacggg atacggcggc aagacgttca gacaggcgtt ttgccgccgc
     4261 gcaggacagg gaataccatg aaacagaaaa aaaccgctgc cgcagttatt gctgcaatgt
     4321 tggcaggttt tgcggcagcc aaagcacccg aaatcgaccc ggctttggtg gatacgctgg
     4381 tggcgcagat catgcagcag gcagaccggc atgcggagca gtcccaaaaa ccggacgggc
     4441 aggcaatccg aaacgatgcc gtccgtcggc tgtaaacttt ggaagttttg aaaaacaggg
     4501 cattgaagga aggtttggat aaggataagg atgtccaaaa ccgctttaaa atcgccgaag
     4561 cgtcttttta tgccgaggag tacgtccgtt ttctggaacg ttcggaaacg gtttccgaaa
     4621 gcgcactgcg tcaattttat gagcggcaaa tccgcatgat caaattgcag caggtcagct
     4681 tcgcaaccga agaggaggcg cgtcaggcgc agcagctcct gctcaaaggg ctgtcttttg
     4741 aagggctgat gaagcgttat ccgaacgacg agcaggcttt tgacggtttc attatggcgc
     4801 agcagcttcc cgagccgctg gcttcgcagt ttgcagcgat gaatcggggc gacgttaccc
     4861 gcgatccggt caaattgggc gaacgctatt atctgttcaa actcagcgag gtcgggaaaa
     4921 accccgacgc gcagcctttc gagttggtca gaaaccagtt ggaacaaggt ttgagacagg
     4981 aaaaagcccg cttgaaaatc gatgccattt tggaagaaaa cggtgtcaaa ccgtaatggc
     5041 atttccaata ccgatgccgt ctgaagcctt ttcagacggc attgcacgtt caggtaagga
     5101 ggacggctta tgcgtgcggt catacagaaa acggtaggtg caaaggtgga tgtcgtgtcc
     5161 gaaaacggca cggaaacctg tggcaaaatc gacggcggat tcgtcgtgct gctcggcgta
     5221 acgcatagcg acacagaaaa agatgcgcac tatatcgccg acaaaatcgc ccatttgcgc
     5281 gtgtttgaag acgaagcggg caagttgaac ctgtctttga aagatgtcgg cggcgcggtg
     5341 ctgctggtgt cgcagtttac actttatgcc gatgcggcaa gcgggcggcg gccttcgttt
     5401 tcccaagccg cacctgcaga acaggcgcag cagctttacc tgcgaacggc ggaactgttg
     5461 cgcggacacg ggattcatgt cgaaacaggg cgtttccgca cgcatatgca ggtgtcgctc
     5521 tgcaacgacg ggccggtaac catactgctg gactctttca tgacgcggat ttccccaaaa
     5581 atgaaggttg ttccggattg aaattgaatc tgcaatgatg aaatatcgac aatgaacgac
     5641 aatacacaca cccttccccc gcgccgcctt tccgtcgccc cgatgctcga ctggacggac
     5701 aggcactacc gttatatggc gcgccagatt acccgcaata cttggctgta cagcgaaatg
     5761 gtcaacgccg gcgcgattgt ttacggcgac aaagaccgct ttttgatgtt caacgaaggc
     5821 gagcagcccg tcgccctgca actgggcggc agcgacccgt ccgatttggc gaaagccgcc
     5881 aaagccgccg aagaatacgg ttacaacgag gtcaacctca actgcggctg ccccagtccg
     5941 cgcgtgcaga aaggttcgtt cggcgcgtgc ctgatgaacg aagtcgggct ggttgccgac
     6001 tgccttaacg ccatgcagga cgcggtcggc atcaccgtta ccgtcaaaca tcgcatcggt
     6061 gtggacaggc agaccgaata ccaaaccgtt gccgatttcg tcggcacgct gcgcgacaaa
     6121 accgcctgca aaacctttat cgtccacgcc cgcaacgctt ggctggacgg tctttccccc
     6181 aaagaaaacc gcgatgttcc gcccttgaaa tacgattacg tttaccgcct caagcaagag
     6241 tttcccgagc tggaaatcat cattaacggc ggcatcacca ccaacgaagc aatcgccgga
     6301 cacctgcaac acgttgacgg cgtaatggtc ggacgcgagg cgtaccacaa cccgatggtg
     6361 atgcgcgaat gggacagact gttttacggc gacaaccacg ccccgatcga atacgccgat
     6421 ttggtgcagc gtctctacac atacagccaa gcccaaatcc aagccggacg cggcacaatc
     6481 ttgcgccaca tcgtccgcca cagcctcggg ctgatgcacg gcttgaaagg cgcgcggact
     6541 tggcggcgta tgctttccaa cgcgacactc ttgaaagaca acgacggcgg cctgattctc
     6601 gaagcgtggg aagaagtcga acgggcaaat acgcgcgaat agggcggggc tgtatgtgaa
     6661 atgccgtctg aaagtttcag acggcatttg tgcgtttgtc gggcggtgtt tagggggtgg
     6721 taatggcgtg tttcggcact ttgtccatat cccagtgtgc caccgcccag tcgagcagtt
     6781 cggcagggcg gtcggtttcc ggtgcttcgg gcagcttgag gtaacggaac acttggcgga
     6841 ggagttgttc gcggcggttt aaatccaatg cgggggcgag cgtctgtttc gaccatttct
     6901 gcccttgtgc gttggtcagc agcggcaggt gggcatattg cggtgtcgga acgcccaaac
     6961 actgctgcaa atagatttgg cgcggagtgg aaacgagcag gtcttgtccg cggacgatgt
     7021 gggtaacgcc ctgttcggca tcgtcggcaa cgacggcgag ttggtatgcc cagtaaccgt
     7081 ctgcacgaag caggacgaaa tcgccgatgt cgcgggcgag gttttgggcg taaccgccga
     7141 cgatgccgtc tgaaaaaccg ataatgcggt cggggacgcg gatgcgccac gccggctgtt
     7201 tgccttgcgg tgcagggcgt tggccggggt ggcggcaacg tccgttatag acgaatccgt
     7261 ctgcgccccg ccttgccccg gcctgccagt ctttgcggct gcaatggcag ggatagacca
     7321 gtccggcggt ttgcagacgg catagggttt cttcatacag ggcgtaacgg tggctctgat
     7381 aggcgacttc tccgtcccac tcgaatccga atgcctcaag cgtgtgcagg atatggcttg
     7441 ccgcccccgg catttcgcgc ggcggatcga ggtcttccat gcggaccagc catttgccgc
     7501 cgtttgagcg tgcatcggca taggaagcgg cggcggtcag cagcgagccg atgtggagca
     7561 gcccggtcgg gctgggggcg aaacgtcctg tgtacatatc cggtacggcc cctttattta
     7621 agactattaa tcaaagccat tatctcatct ttattcagtt ccatcccggg ttcttcaagt
     7681 aaggttaaat catatagggc attatattgc tcttcggtag ctgaaccatc cataaggccg
     7741 gacgagaaaa aatcgaaggc tctatctgcg attctctcat tacttgcatt tctactaacc
     7801 agtttcatca attctgtata tattgtatgg ggaggtaccg gatccttaaa aatcagatct
     7861 gaataaattt cattgggtga aatgatttcg agtgcttttg ccatgattct atttcctttt
     7921 gtgttggtgg gtaatgtcgt gcattaactt cttgttgatt aatattttta gggcgaatcc
     7981 ttgatatgcc gcactgtgtc cggtcaaaag ggcgatgccg tctgaaagcc tttcagacgg
     8041 catcggggat gaaaagttta aaccaacggt gcgagcagtt tttcaaacgc ttcttcaaac
     8101 tgtttcaaac cgtcttcctg caaacgcgtt gccaaggttt cgacatcgat gccgagcgcg
     8161 gcggtttcgg cgagctgcgc ttgtgcttct tctacgcctt cggtcagcgt ggctttggct
     8221 gtgccgtggt cgataaaggc tttgagcgtg gcatcgggaa cggtgttgac ggtgtgcgcg
     8281 ccgatcaggc tgtcaacgta gagcgtgtcg ggataggctg ggtttttcac gcctgtggat
     8341 gcccataaaa gctgcacgcg gtttgcgcct ttggtttcca gcgcggcaaa ttccgggctg
     8401 ccgaagtatt gcgcccagtc ttggtaggcg gctttggcaa gggcgatggc gattttgcct
     8461 ttgaggtggt cgggcagtgt tgtgtccagc gcgccgtcca cgcgggagat gaagaagctg
     8521 gcgacaactt gaatatgggc aatgctttgt cctgctgcta aacgtttggc gatgccgcgc
     8581 gcgtaggctg cgtaggcttt gagggtttgg gcgcgtgaga acagcagggt caggttcacg
     8641 ctgatgccgt ctgaaacgag ggtttcgagc gcatcgatgc ctgcgtcggt ggcaggcact
     8701 ttaatcatcg cgtttttgca cccgatggcg gcgtagaggc ggcgcgcttc ttcaaccgtg
     8761 ccttgcgcgt ctttggacaa ttcgggcgaa acttcgaggc tgacgaagcc ggttttgccg
     8821 ccggtggatt cgtgttcggc aagacagacg ccgcaggcgg cacgcacatc ggcaaccgcc
     8881 atcgtttcgt agcgttgttt gggggtcagg tcttgctgct tgagggcggc aacctcgtcg
     8941 gcgtaaagcg catcgccggc gaaggctttt tgaaagatgg cgggattgga agttacgccg
     9001 cacacgcctt gtttcagcat ttgcgccaat tcgccgcttt gcacgagcga gcgggaaagg
     9061 ttgtccagcc agatttgttg tcctaatgct ttaacgtccg ataaaatagt catttctgat
     9121 tcctttggat ggatgtgtat gtttcaaagg cttatgctac cccgattcgg aaattttggg
     9181 tagttttatt acagcaaagg cggatggcga tggcaggaaa cgaaaaatat cttgattggg
     9241 cacgcgaagt gttgcacacc gaagcggaag gcttgcgcga aattgcggcg gatttggacg
     9301 aaaacttcgc ccttgcggcg gacgcgttgt tgcactgcaa aggcagggtc gttatcacgg
     9361 gcatgggcaa gtcgggacat atcgggcgca aaatggcggc aaccatggcc tcgaccggca
     9421 cgcccgcgtt tttcgtccac cctgcggaag cggcacacgg cgatttgggc atgattgtgg
     9481 acaacgacgt ggtcgtcgcg atttccaatt ccggtgaaag cgacgaaatc gccgccatca
     9541 tccccgcgct caaacgcaaa gatatcacgc ttgtctgcat caccgcccgc cccgattcaa
     9601 ccatggcgcg ccatgccgac atccacatca cggcgtcggt ttccaaagaa gcctgcccgc
     9661 tggggcttgc cccgaccacc agcaccaccg ccgtcatggc tttgggcgat gcgttggcgg
     9721 tcgtcctgct gcgcgcacgc gcgttcacgc ccgacgattt cgccttgagc catcctgccg
     9781 gcagcctcgg caaacgcctg cttttacgcg ttgccgacat tatgcacaaa ggcggtggcc
     9841 tgcctgccgt ccgactcggc acgcccttga aagaagccat cgtcagcatg agcgaaaaag
     9901 gtttgggcat gttggcggta acggacgggc aatgccgtct gaaaggcgta ttcaccgacg
     9961 gcgatttgcg ccgcctgttt caagaatgcg acaattttac cggtctttcg atagacgaag
    10021 tcatgcatac gcagcccaaa accatctcca ccgaacgcct cgccaccgaa gccctgaaag
    10081 tcatgcaggc aaaccatgtg aacgggcttc tggttaccga tgcagacggc gtgctgacag
    10141 gtgcgctgaa tatgcacgac ctgctggcgg cacggattgt atagtggatt aactt
//
LOCUS       DAXHRI010000059        10093 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXHRI010000059 DAXHRI010000000
VERSION     DAXHRI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10093
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.059"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(51..245)
                     /locus_tag="ACFM75_001105"
     CDS             complement(51..245)
                     /locus_tag="ACFM75_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC6255663.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(235..2316)
                     /locus_tag="ACFM75_001106"
     CDS             complement(235..2316)
                     /locus_tag="ACFM75_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC6255664.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2793..3173
                     /locus_tag="ACFM75_001107"
     CDS             2793..3173
                     /locus_tag="ACFM75_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC6255665.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTADF
                     RAFSCENGLSVHVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3456..4088)
                     /locus_tag="ACFM75_001108"
     CDS             complement(3456..4088)
                     /locus_tag="ACFM75_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255666.1"
                     /translation="MEKIEKFKSELLDDIFEYIQCISIFVHKKKIYYLIDYKENFILN
                     MKLDLDFDFKNGNITLEQYQDEINSCYYRNGIWQLTKDNFESYLQSDSVIVLKKDELK
                     ELMFQGFTSDEAVRLYSVVENKLSYNDPISDSGRQSDFLKINQISSRLPLFYVNFDTE
                     VYLHMDWDRCHEDYVYDGWFAKAMDFGYLIPDEFCYWKIEGRDYWKFGQL"
     gene            complement(4242..4735)
                     /locus_tag="ACFM75_001109"
                     /pseudo
     CDS             complement(4242..4735)
                     /locus_tag="ACFM75_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            5067..6500
                     /locus_tag="ACFM75_001110"
     CDS             5067..6500
                     /locus_tag="ACFM75_001110"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC6255667.1"
                     /translation="MNEYSQLIKHPDISLSPISDSIGIGNPATGEILAYVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIIKPASLTPLSAYALASLAYEAGIPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            6609..7397
                     /locus_tag="ACFM75_001111"
     CDS             6609..7397
                     /locus_tag="ACFM75_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC6255668.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            7765..9321
                     /locus_tag="ACFM75_001112"
     CDS             7765..9321
                     /locus_tag="ACFM75_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC6255669.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            9338..10084
                     /gene="surE"
                     /locus_tag="ACFM75_001113"
     CDS             9338..10084
                     /gene="surE"
                     /locus_tag="ACFM75_001113"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC6255670.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         2430 a         2530 c         2744 g         2389 t
ORIGIN      
        1 taaaaattcc gtctgaagcg gcgttcagac ggaatttgtt tcaagcaggc ttaacagcag
       61 cgtccgccgt ttgcgccgca gcggcgtttg cggcaatagt cttgaaactg cagcttggtc
      121 atcacagggg cgttgggatt atgtttgcgc tgctgtgcaa cgtagttttc ataatcgggc
      181 acgcctgcca tcaagtttgc tgtcagcttg atggttttcc accaagacgc gagcttatgc
      241 ttcactttgt gcctccggct gtttgccgtc gcggtacacc gccgggattt ctttggcggt
      301 cggccagccg actttgcgtg ctttgagggc ggtacgcaaa ccgtacgcgg cgacaatcac
      361 gacaaccgac aagaagagga tggtcagacc ggcattaatc ttgtcgttga agatgatttg
      421 cgccatttcg ccgatgtctt tggcaggcgc aaggatttcg tttttagcca atgcgtcgct
      481 gtatttgccg gcgtgggcaa ggaagctgat gcgcgggtcg ctgtggaaca gtttttgcag
      541 gccggcgtag caggttacga acagtacgcc gacggcggga acgagtacca cccagacata
      601 acggtcgcgt ttcatcttaa tcagcaccac ggcgcacata atcaaggcta cgcctgccag
      661 catttggttg gcgatgccga acaaaggcca gagcgagttg atgccgccca acgggtcggt
      721 cacgcccgtg tagaggaagt agccccacaa tgccacggcg aagaaggtcg caatcaggtt
      781 ggcggggatg gagtcggtgt tgccgaaagg tttgtagaag atgctgccca agtcttgaat
      841 cataaaacgt gcgacgcgcg taccggcatc gacggcggtc aggatgaaca aggcttcaaa
      901 caatagggcg aagtgatacc agaacgccat catcgcctcg cccggaatca ggcggctcat
      961 aatgtgcgcc ataccgactg cgagggtggg cgcaccgcct gcacgggaaa ggatggtgtt
     1021 ttcgcccact tccttagcgg tgtgcaacag ggttgcagca tcgacaggga attgcagctt
     1081 ggtggtaatc acttcggcgg cggtattggc atccgtaccg atcagggcgg cagggctgtt
     1141 catggcgaag tacacgccgg gatcaagcga tgcggcggcg gcaagtgcca taatggctac
     1201 gaaactttcc atcaacatac cgccgtaacc aatcatacgg acgtgggttt cgttttccag
     1261 cattttcggc gtggtgccgg aggaaatcag cgcgtggaag cccgaaaccg caccgcaggc
     1321 gatggtgatg aagaggaacg ggaacaatgc gcctgagaat accgggccgg aaccgtcgat
     1381 aaagtgggtt acggcaggca tttgcaaagt gggattgacg atgacgatgc ccaaagccaa
     1441 ggccgcaatc gtaccgattt tcaggaaggt ggagagatag tcgcgcggag tgagcagcag
     1501 ccatacgggc aatacggagg cgacaaagcc gtaaatcata atcgcccaag tgagctggat
     1561 gccgtcaagg tcgaaccaat gtccgatgga acttttcgcc acatcttcgc cgtaaattac
     1621 cgccagcatc agcaaaataa agccgacgat ggaaatctcg ccgattttgc ccggacggat
     1681 atagcgcgtg taaataccca taaacagggc aatcggcatc gttgcggcga tggtaaacgt
     1741 accccaaggg ctgtgaacca gtgcttttac gacaatcaac gccaacaccg ccataatgat
     1801 gaccataatc atcaaaatac cgatggaggc aatcacgccg gggacagtgc cgagttcctg
     1861 tttcacaata tcgcccaaag acttaccgtc gcggcgcata gagacgaaca agaccatcat
     1921 atcctgtacc gcgccggcaa acaccacgcc gaagataatc cacaaagtac cgggcaggta
     1981 acccatttgc gccgccaaaa ccgggccgac caaagggccc gcgcccgcaa tcgcggcaaa
     2041 gtggtgtccg aacaatacgc ctttgtgcgt cggaacgtag tccaagccgt cgttgtggcg
     2101 ttctgccgga gtcaggcggt taggatcgag ccgcattacg cggttggcga tgtagaggct
     2161 gtaaaaacgg taggcgatgc agtaaacgga tatggcggcg gtaaccatcc ataccgcgct
     2221 gacctgttcg cctcggctga gagccagagt ggtaaaggat gctaagccga ccagtaccac
     2281 tatgccccaa atgaggaagg ttttgagtga tttcatcgaa taaatcctta tctcacactg
     2341 tcggaatatg cctgaacggc gggaaacggc cgaaccatct gccggcacgg gcacaaaccg
     2401 gagtctgaat gattgacgaa gtatgaaaca gtgcgcttgt cgggatttgt gccttatgcc
     2461 cgcatcaaac agtgcaggga tgcgggcgca caaaacgtta aacgccgaat aggattttaa
     2521 cgcaaattag cacactgata gcggttttta cttggaattt ggaaaaaatt tacattcctt
     2581 cgggcggaca ggcgggtttg gacggcatcg tcaggcaaaa ggcggcgccg gaagacgggt
     2641 aaagaagggg cgcgcaatcc ggattattga ttcatcgcgg gtaaattccg gttatcgggc
     2701 ttgtgtgttt gcgcgtccgt ttatagtatg gcgttgccgc agcttggaat cagggcggtt
     2761 gtttcatatc ttatttttat tggggagctt ttatgaatat caggtttttc gcgctgaccg
     2821 taccggtttt gtctttggcg gcctgtgccg tgccggaggc gtatgatgac ggcggacgag
     2881 ggcatatgcc gcccgttcaa aaccaagccg gcacggcaga ttttcgggca ttttcctgcg
     2941 agaacggttt gtctgtgcac gtccgccgtt tggacggcgg cagaatcgcg ttgcggttgg
     3001 acggcaggcg tgccgtcctc tcttccgacg ttgccgcatc cggcgaacgc tataccgccg
     3061 aacacggttt gttcggaaac ggaaccgagt ggcatcagaa aggcggcgaa gcctttttcg
     3121 gctttaccga tgcctacggc aattcggtcg aaacctcctg ccgcgcccgc taaacggggc
     3181 agcctgtttg aaccgccgtc cggatttttc gggcggtttt ttatgtcgga tttgttttgc
     3241 agttcggcct ccggcagggt tttggctgtc tgataccgtc cgtaacttcg ggtattgccg
     3301 agtttgtcgg caacgaccgc tgcgacttgg gcggcgtttt gccgaacgcc tgcgttactt
     3361 ccctttgtag gccgatctct ttggcaaccg cgtctttgtc gaagctgttt ttcagacggc
     3421 cttttgaatt catacaatct atggcttgct tatctttata attgtccaaa tttccaataa
     3481 tcccgccctt caattttcca ataacaaaat tcgtccggta ttaaataccc aaaatccatc
     3541 gcctttgcaa accagccgtc ataaacataa tcttcatgac atctgtccca atccatatgt
     3601 aagtacactt cagtgtcaaa attgacatag aataaaggca gcctcgaaga aatctgattg
     3661 atttttaaga aatcactttg ccggccggaa tctgagatag gatcattgta cgacagcttg
     3721 ttctcgacaa cggaatataa ccttaccgct tcatcagaag taaacccctg aaacatcagt
     3781 tccttcaatt catccttttt taatacaatg actgaatctg attgaagata gctttcaaaa
     3841 ttgtccttag ttaattgcca gataccgttt cggtaataac aactatttat ttcatcctga
     3901 tattgttcta acgtaatatt cccgttttta aaatcaaaat ctaaatccaa cttcatgttt
     3961 aatatgaaat tttccttata atcaatcaaa taatatatct ttttcttgtg gacaaaaata
     4021 gatatgcatt gaatgtactc aaaaatgtca tccaataatt cagatttaaa tttctcaatc
     4081 ttttccatat ttgccaaatt ccttaataac tttatcttca gcaagccgta acccacatta
     4141 aatctgcagg taaggtgtgg tacgcacgcc agtgaacttg agtctaataa aaaggtcgtc
     4201 tgaaacattt ttccggcttt cagacggcct tttgccaaat attatcggca gcggctcaat
     4261 gccaacttta aacctgctcc gatttcttca gggctgttat ccaatgataa aattacatcg
     4321 tctgcatcaa tggcatccca cgcttccagc ttgacatggc ggctcgggct gattttcagg
     4381 cagccgttgt gcagccaaat atccacgctc atcatgtttt taaatagggc gcgtctggtt
     4441 ttatagccca agttcctgca taggttggca acccaatcct catagcgttg ccgaaatttt
     4501 tcggtatcaa aaaaatcttg gtcttctgga ctgtcataaa cgaaagtcct gctgtttgcc
     4561 aacgcttgca agaccgtcgt gcctaaagtt tcattgtcgg tatccaatgg caggatatgg
     4621 ggggatatag gtggtctatt gccgttaatc ccaaacctga acatgtttga acaatcagag
     4681 cttcttcatt cgtataaaaa actgcccaat aattttgatc ctgtttaaaa atcattattc
     4741 tatctccgta attttgactg taatgtttcg atttttgcca tactctacca cacgttgtaa
     4801 ctgcaatctt tgctccttat atattgcacc ccatcaaagg gctgcattac ttttctttct
     4861 aaagattacc attatataaa aagtattttc ttaaacgaca gggattgcgc ctgcaccata
     4921 ttcagacacc acccggatgt tgcgccgtcc gatcggatgc ttcagacggc atcggaaggg
     4981 tttgcagttt tggaatatga agatgataat gtccgcgaga ttgacggcat ttgaaagcgg
     5041 ttatcccgca taccggagga cacgaaatga acgaatattc ccaattaatc aagcatcccg
     5101 atatttccct ttccccgatt tcagacagca tcgggatcgg caatccggcg acgggtgaaa
     5161 ttttggcgta tgtccgcaag acggattcgg acaagctgaa aaacctgatt caaaaagcag
     5221 ctgcggcaca aaaattatgg gcggcgaaaa ccgctttgga acgcgccgac atattgtggc
     5281 gttggtattt tttagttaaa gaaaacaagg aagcactggc gcgcctgatg acgatggagc
     5341 agggcaaaag cctgaccgag tcgcgcggcg aaatcgatta tgcggcttcg tttgtgcgct
     5401 ggtttgccga agaggcgcgg cggattgacg gcgatgtgct gacaagcgtg aaagcgtcgc
     5461 aaaaactggt cgtgttgaaa cagcccgtcg gcgttaccgc tgcgattacg ccgtggaact
     5521 ttccgtccgc gatgattgcg cgcaaggccg cgcctgcttt ggcggtgggt tgcgcgatga
     5581 tcatcaaacc cgcatcgctc acgcctttga gtgcgtatgc cttggcttcg ttggcttacg
     5641 aagcgggcat accgcaggat ttgttgcccg tcgtcagcgg ccgcgcatcg gaaatcggcc
     5701 gtgaatttgc cacgaacccg attatccgca aaatcagctt caccggttcg accgaagtcg
     5761 gcgcgaaaat ttttgccgac agcgcggcgg acattaaaaa actcagtttg gaactgggcg
     5821 gcaacgcgcc gtttatcgtg tttgacgatg ccgatttgga caaagccgtc gaaggtgcgc
     5881 tcgccagcaa gttccgcaac agcggtcaga cctgcgtctg caccaaccgc gtttacgccc
     5941 aatccggcat ttacgacgaa ttttgtcgca aattgagtga aaaagtagcc gagctcaaat
     6001 tgggcaacgg cttggaggat ggcgtcaacc aagggccgct gattgaggaa aaagcggtag
     6061 aaaaagtcga gcagcacatt gctgacgcgc tcgctaaagg cgcggtctgt ctgaccggcg
     6121 gcaaacgcag cgcattgggc ggaacgtttt ttgagccgac cgtcttaagc ggcgtaacgg
     6181 cgcaaatggc ggtggctcgc gaagaaacct tcgggccttt gtgtccggta ttccgttttg
     6241 aaaccgaagc cgaagtcatt gaggctgcga acaatacgga atacggtttg gctgcttacc
     6301 tttttaccgc cgacaccgca cgccagtggc gcgtcggcga agcattggaa tacggtatgg
     6361 tcggcatcaa tacgggctta atcagtaatg aagcggcacc gttcggcggc gtgaaacgca
     6421 gcggtttggg acgtgaaggc agcaaatacg gtgcggacga atatctggaa ttgaaatatc
     6481 tgtgtatgga tgtcgggtga cggatgccgt ctgaacggca gggtttcaga cggcgccgca
     6541 ttttaagcag tctctatctg ttgtacaatg cgccctgttt ttacggttat ttttgatttg
     6601 aacaagatat gatggaaaac ggaaaaacag tcccgagatg gcgttttgcc ttgaaaagtg
     6661 cgggctggca tctcttaatc agcctgtcgg ttgcagggct ggcggcattg ctggttttta
     6721 aggtttggta tccttatcct tatgccgagc tgacgggagg gctgtcgctt tatcagctgg
     6781 tggtggctgt cgatattgta tgtggtccgc tgctgacttt aattttggca agcccgaaga
     6841 aaaagacaaa ggcacgcatg gtcgattttt ccatggtcgg catcatccag ctggcggctt
     6901 tggtgtacgg tctgcacagc gtttctttgg cgcgccctgt gatagaggcg tttgaaaaag
     6961 acaggattaa tgttgtgacc gccgccgaag tggcggttga agatttgcac aaagcgccgg
     7021 aagggctgca aagcctgtcg tggttcggca tccgccgcat tgcattgaaa gaacctgagg
     7081 atgcggatga gaagaacaag acgctggatt tgtccctgaa aggtatcgag ccgagtatgc
     7141 gtcccgacca gtggctgccg tattccgaca aggaagcaga agaaatccgc aaacatctga
     7201 aaccgctgaa agtcttggcg gatgcgagaa aaacgacggt tgcggacatt ctgaaacagg
     7261 caggtctcgc cgaaggggag gagctgtatt acctgccgtt taccagcagc aggcagaaag
     7321 agtggatagt cattaccgat aaagagggca acaccaaagg ctacgcgccg atagacggct
     7381 tcatcatcac cccttaagcg ttgggactcc gtccgcactc gaacatccgt tcttcgcggc
     7441 ggtagaatca gactgtattt gagaggggcg caacccctca atcagggcaa cacatagggg
     7501 aaggctttat gtgccgtccg tgtgttgaaa catttcaaaa taagctcatg ccgtccgaac
     7561 atcctttcag acggtatggc gttttgccat tgccccgata agctgttaaa ctatttaaat
     7621 tatttcgacc gaaggtacac gcacatgcaa gaccccgagc agagcagcaa gcccgcccgc
     7681 cgttttttgt gcgtaccgtc tgtttaggca gcttgagcgc ttcaggctgc cttttcgcgt
     7741 ttgaacttga ggaatacgaa aatcatggat ttcagttggt tggcagaacc gcatacctgg
     7801 ataggttttg ccacgctttt ggtgttggaa gtcgtattgg ggatagacaa tcttgtcttt
     7861 gtggcgattt tggcaaacaa ggtccagccc gcacggcgcg accgcgcacg gattatcggg
     7921 ctggggctgg cagtcgtcat ccgcatcatt atgcttgctt ttatggcgca catcatcacg
     7981 ctgaccgagc cgctgttcca aatcggcggc ctcgccgttt ccggcaagga catgattatg
     8041 ctcgcgggcg gtattttcct gctttacaaa gccaccaccg aactgcacga acgcctcgaa
     8101 gggcacaacc ggtttaccgt tgccgacagc cagaaaaaac acgcgccgtt ttggggcgtg
     8161 gtcgcgcaaa tcctgatact ggatgctgtg ttttccatcg attcggtcat caccgccgta
     8221 gcgatggtcg atcacatcgt tgtggcgatg ggcgcggtcg tcgtcgcgat ggcggtgatg
     8281 atttccgcca gcaaactctt gaccgaattt gtagacaggc atcccaccgt cgtgatgctc
     8341 tgccttggtt ttttgttgat gatcggtttc agcctgattg ccgaagcctt ccatttccac
     8401 attcccaaag gctacctcta cgccgccatc ggcttctcga ttttaatcga attgtttaac
     8461 cagatttcgc agcgcaacag ccgcaaaaac gactacatcg gcagctcgtg gcgcaagcgc
     8521 accgccgaaa acgtcttggg tatgatgggt atacgcgaaa gcgtgcttgc cgacgcgggc
     8581 ggcgaatccg gggacgacgc gcattttgaa gaaaacgaaa aatcgatgat acgcagcgtg
     8641 ctgacgcttg ccgaacgccc gattatgggg gtgatgatcc cacgccgcga catcgaacgg
     8701 ctggacattt cccaaagccg cgaagaacag tgtgcccaac tgcaaaacac gccttacagc
     8761 cgcctgctcg ttgtcggtaa ggcgggcgtg gacgaacctt tgggctacat caacaaaaaa
     8821 gacctgctgt cccaactgct ggaaacaggc ggtctcgaca ttcagacggc attgcgccag
     8881 ccgctcgtcc tgcccgacag caccaccgcg ctgggcgcaa tcgaactctt ccgccaaagc
     8941 agcgcggatt atgctttggt ggtggacgag ttcggcgcgg tattgggcat ggtaaccatg
     9001 aaagacctgc tcgaaaccat cgcaggcgag ttccccgaag aatttgagcg cgaagaagaa
     9061 ccagccgttc aggggaatcc cgatgaaagc ctgacggtgg aaggcgcgtt ggaatatgtg
     9121 gaactcgcac cgcaactcaa cctgccgcag caggaggaag atgccgattt ccatacggtt
     9181 gccgggctga ttatggaaga attgcaaacc atccccgatg tcggcgattt tgccgatttc
     9241 cacggctggc ggtttgaagt ggtcgaaaaa gaagggcagc gcatcgagcg ggtcaaaatc
     9301 accaaattgc ccgaagaata agcattcagg atagaaaatg aacgttttga tttccaacga
     9361 cgacggctac ctctccgaag gcattgccgt tttggcgcgc gttacggcgg aatttgccaa
     9421 cgtcagggtg gttgcgcccg aacgcgacag gagcggggtc agcaattcgc tgacgctgga
     9481 acgccctttg cagttgaaac aggcgcaaaa cgggttctac tatgtcaacg gcacgccgac
     9541 cgactgcatc cacatcgggc agtctgtatt ttcggatttt caggccgatt ttgtcttttc
     9601 gggcatcaac cggggcgcga atatggggga cgacacgctt tattcgggga cggttgcggc
     9661 ggcaaccgaa gcctacctta tgggcatacc cgccgtggcg ttttccttaa acgacgcttc
     9721 cggacgctat tgggcgaccg cagaacaggc actgtggaca ttgttggcgc attttttcaa
     9781 aaaccccccg cagtccccta ttttatggaa catcaatatc cccgccgtcg cgccggaaga
     9841 tgtgcggggc attaaaatcg cccgtttggg caggagacac cacggtcaga acgtcatccc
     9901 cgcgcgcaat ccgcgcggcg aacagattta ttggatagga ccggtcggcg aagtttccga
     9961 tcgggaagag ggaacggatt tcggtgaatg cggcgcaggt ttcattaccg taacgccgct
    10021 gcaaatcgac ctgaccgcct atccggacat ggcggaaaca gcggcgttct ggcatgcgga
    10081 ctgaccgttt cat
//
LOCUS       DAXHRI010000060        10018 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXHRI010000060 DAXHRI010000000
VERSION     DAXHRI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.060"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(133..1350)
                     /locus_tag="ACFM75_001114"
     CDS             complement(133..1350)
                     /locus_tag="ACFM75_001114"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC6255671.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRVDLADGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1552..2280)
                     /gene="rph"
                     /locus_tag="ACFM75_001115"
     CDS             complement(1552..2280)
                     /gene="rph"
                     /locus_tag="ACFM75_001115"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC6255672.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHADGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2362..2826)
                     /locus_tag="ACFM75_001116"
     CDS             complement(2362..2826)
                     /locus_tag="ACFM75_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC6255673.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3043..3819
                     /locus_tag="ACFM75_001117"
     CDS             3043..3819
                     /locus_tag="ACFM75_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6255674.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4064..4918
                     /locus_tag="ACFM75_001118"
     CDS             4064..4918
                     /locus_tag="ACFM75_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC6255675.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFISLLELAKEGLVRIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4959..6167
                     /gene="pncB"
                     /locus_tag="ACFM75_001119"
     CDS             4959..6167
                     /gene="pncB"
                     /locus_tag="ACFM75_001119"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC6255676.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6259..7977
                     /gene="argS"
                     /locus_tag="ACFM75_001120"
     CDS             6259..7977
                     /gene="argS"
                     /locus_tag="ACFM75_001120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC6255677.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDAAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDSAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8504..9922
                     /locus_tag="ACFM75_001121"
     CDS             8504..9922
                     /locus_tag="ACFM75_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6255678.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPTPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGPMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2301 a         2953 c         2520 g         2244 t
ORIGIN      
        1 aatccactat aaatttaatc cactatattt tttgttccaa agtcaaaata tgccgtccga
       61 acattcgggc ggcagacaaa acggcactgc ccgataaagg cagtgccgtt gtccgtttca
      121 aaccgtgaaa catcagccca aattaaaggc tttatgcaat accctggttg ccagttccat
      181 gtatttttca tcaatcaata cggaaacttt gatttcggag gtggaaatca tttggatgtt
      241 gataccctct tcggcgagcg tgcggaagat tttggcggct acaccgacgt gcgaacgcat
      301 acccaaaccg actgcggaga ctttgcatac ggtgtcgtcg ccatcaatag aagccgcgcc
      361 gatactgtct tggcgttccg acaggatttc caaagtctgc ttgtaatcgc cgcgcggtac
      421 ggtaaaggaa aaatcggttg tgccttcgct gccgacattt tggataatca tatcgacttc
      481 gatgttggca tcggcgaccg cgcccaaaat ctgataggcg acaccgggtt tgtcgggtac
      541 gccgcgcacg ttgatgcggg cttggttttt atcgaatgcg ataccggtta cggcagctct
      601 ttccatgttg tcgtcctctt caaaggtaat taaggtgcca ttgccgccgt cttgcaggct
      661 gctcagtacg cgcaggcgca ctttgtactt tccggcgaat tctactgaac ggatttgcaa
      721 gactttcgag ccgaggcttg ccaattcgat catttcttca aatgtaaccg tatccatgcg
      781 gcgcgcttcg ggtacgacgc gcggatcggt ggtgtacacg ccgtccacgt cggtatagat
      841 ttggcattcg tccgctttga gggctgcggc gagcgcaacg gcggaagtgt cggaaccgcc
      901 gcgtccgagc gtggaaatat tgccttcact gctgatgcct tggaagccgg caacgatgac
      961 gactttgcca tcggcgaggt cgacgcgcat tttttcgtca tcaatgcttt cgatgcgggc
     1021 tttggtgtgg gcggtatcgg ttttgagggc gacctgccag cctgtgtagc ttttggcatc
     1081 cacgccgatg tctttcaatg ccatcgccaa aaggccgatg gttacttgtt cgccggtgga
     1141 caaaaccacg tccaattcgc gcggatcggg atgctcctgc atttcgtgcg ccaacgcgac
     1201 caggcggttg gtttcaccgc tcatggcgga tacgacgacg acaatgtcgt gtccttcggc
     1261 gcgggctttg gcgacacgtt tggccacgtt tttaatgcgt tcgggcgagc ctacggatgt
     1321 gccaccgtat ttgtgtacga ttaacgccat gtttcgtgct ttcttgtgtg ggattggtcg
     1381 ggcagcatgg tttgctggaa aagcggctat tattactatt ttttacatga agttcaagaa
     1441 cggactgcgc tttcccacct gccgtttgac agcggtcagc aaaaaacctg ttctttcaga
     1501 ttgttgacaa aatgccgtct gaacggtttt cagacggcat ccggacgaca atcaggcggc
     1561 agacagcgca ttttgctggt gttgcagcag ttcgcctatg cctttttgcg ccagtgcaac
     1621 cagtttgccc aattcgtcca aactgaacgg cgcgtcttcc gccgtacctt ggatttcgat
     1681 gatttttccc gatgcggtca tgacgatatt cacatcactg tcgcaaccgg agtcttcggg
     1741 ataatccaaa tccaaaagcg gcacgccgtt cactacgcct actgacacag cggcaacggc
     1801 ttcgcggatg gggttttcac tcaaaatgcc gtctgaaacc agtttgccga cggcgatttg
     1861 cagcgcgaca aacgcaccgg taatcgaagc cgtgcgcgta ccgccatctg cctgaatcac
     1921 atcgcagtca atcaagattt gtcgttcgcc gagtttttcc atatccacga ccgcgcgtag
     1981 cgaacgcccg ataaggcgtt ggatttcttg tgtgcgcccg gactgtttgc ccgccgaagc
     2041 ttcgcggcgc atacgcgaag cggtggatgc aggcagcatc ccgtattctg cggttaccca
     2101 gccttggttt ttgccgcgca aaaacggtgg gacattttcg tctatggaag cggtacaaat
     2161 cactttggta ttaccgcatt caataaggca cgaaccgtcg gcgtgcggca ggaaatgagg
     2221 ggtgattttg atatcgcgca ggctgtcggc ggcgcgcgag atgcggatgt aatcaggcat
     2281 actgccctcc cgttaaaaac agataaatta aaaagcctta aatatgaaaa atcacattta
     2341 aggccttcaa actgaaaatt tctacgcctc ttcggctttg ctgcggataa tcaaaagcgg
     2401 caggtggctt tggcgcatta ccgtttcggc aaaactaccc attaaaaggt gcatcagtcc
     2461 ggtacgtccg tgcgtaccca acaccagcag gtcggcaccg ttttcatcgg cataatcaac
     2521 caaatcctgc gccatttcac gcgcaccctt attggcaacc agcaggtgtt tgacggtatt
     2581 ttccacaccc agttcccggg cggtgcgctc ggcggcatcc aaaacttcgt tgccttgcgc
     2641 gacggcggcg gcttcgtagc tttcgtgttg caaaaattcg ggggcgagcg ccatatattc
     2701 ggcaggattg gcaacgtgta ccaaagtcag acgcgcaccg ttgaccccgg caagctcggc
     2761 ggcatgtttc agggcattga tggacgtttc actgccgtca acggcaacaa ccagatgttt
     2821 gtacatatcg tattctcctt ttgcaccgcc tcgcggtgcc ctcttgtcgg atgggcgcag
     2881 ggacagtttg cgccgtttca ttatagaccc gccgtcgggc tttatacaac agccgaacag
     2941 cccgaccgct ttccagtata atatgccgct tccgtgcagt caggcatttt ttgccggctt
     3001 tcgttcactt tttgatttga cgcaatcttg caggattcga ccatgtccga caacgctttg
     3061 acctcttcgc gacgcttcgg cggcatcgcc agactctacg gagactccgc cttggcgcac
     3121 ttttcacagg cacacgtctg cgtagtcggc gtgggcggtg tcggctcgtg ggcggtcgag
     3181 gctttggcgc ggacgggcat cggacgtttg actttgattg atttggacaa cgttgccgaa
     3241 tccaatgtca accgccagct gcacgccctg accggagact tcggcaaagc aaaagttacc
     3301 gccttgcgcg aacgcgtcgc ccaaatcaac ccagaatgcc aagtgtttga aattgaagat
     3361 ttcgttaccg aagacaattt gccggaatac ttcggaaaag gttttgattt cgtcatcgac
     3421 gcgatcgacc aagtgcgcgt caaagcagca atggcggctt attttgtgga acgcaaacaa
     3481 ccgtttgtcc tcagcggcgg cgcgggcgga caaaaaaatc cggcgttaat ccaaaccgcc
     3541 gatttgagcc gcgtaaccca cgacccgctg cttgccaacc tgcgctacac cttgcggaaa
     3601 cgctacggat tcagccgcga tacgaaagca aatatgcgcg tgccttgcgt gtattcgacc
     3661 gaaaatatcg tgccgccgca gtctagggag gcttgttcgg cagatgccgc tccgcaaggc
     3721 ttgtcgtgcg ccggctacgg tgcaagcatg ctcgttaccg cttcgttcgg gctatattgc
     3781 gcacaggcgg cggtggaaca catcgcagac aaaaaataag caatgccgtc tgaaacagga
     3841 ttcagacggc atttgaacaa acgcggcaat caagctgtcg gttcagaaaa accaacatcg
     3901 gcacgcctaa aaccgtattc acaacctgct ccttttcaaa acatttgcat ttaaaagccg
     3961 ttataatgcc gtctgaacat ttgcccgacc acattatacg tgaatgtcgg cagattgttt
     4021 tcttttgtaa acttatatta aaatccactt accgattcac gccatgccgc ccatccctgc
     4081 cccatccgca ccatccgagc acactgtcgc atgggtattc ggccaacccg ttaccgattt
     4141 gccgcaggat ttgtttatcc cacccgacgc gctgaaagtc gtattgggca gttttcaagg
     4201 ccctttggat ctactgctgt atctgatccg caagcagaac atcgatgttc tcgatattcc
     4261 gatggtgaag attaccgagc agtatctgca ctacatcgcc caaatagaaa cctatcagtt
     4321 tgatttggcg gcggaatatc ttttgatggc agcaatgctg attgaaatca aatcgcgcct
     4381 gctgctgccg cgtaccgaag ccgtcgaaga cgaagaagcc gacccgcgtg ccgagttggt
     4441 gcgccgcctg ttggcttatg aacagatgaa gctggcggca caagggttgg atgcgcttcc
     4501 tcgtgcgggc cgggatttcg catgggcgta cctacctctg gaaattgccg tcgaagccaa
     4561 gctgcccgaa gtctatatta ccgacttgac gcaagcgtgg ctgggtattt tgtctcgggc
     4621 aaaacatacg cgcagccacg aagttatcaa agaaaccatc tccgtgcgcg cgcaaatgac
     4681 ggcaatcctg cgccgtttga acggacacgg gatatgcagg tttcacgacc tgttcaatcc
     4741 cgaacagggc gcggcttacg tggtcgtcaa cttcatctca ctgttggagc ttgccaaaga
     4801 aggattggtc agaatcgtgc aggaagtcgg tttcggagaa atccgaatca gcctcaatca
     4861 tgagggggcg cattcagacg gcatttccgg cacacgaggc gggcgcgatg tgttctaata
     4921 cgccccaagc cgccaccaaa aatcgggaga cacgccatat gaccggcatc atacattcgc
     4981 tgcttgacac cgacctctac aaattcacta tgctgcaagt ggttctgcac cagtttccgc
     5041 agacgcacag cctttacgaa ttccgctgcc gcaacgcctc gaccgtctat ccgcttgccg
     5101 acatcaagga agacttggaa gccgaactcg acgcgctctg ccggctgcgc ttcacccacg
     5161 acgaactcgg ctatctgcgc tccctgcgtt tcatcaaaag cgactttgtc gattatctcg
     5221 aactcttcca gctccaacgc cgctttgtcg aagtcggcac agacgataaa ggccgtctga
     5281 acatccgtat cgaaggcccg atgatacagg cgatgttctt tgaaatcttc atcctcgcca
     5341 ttgtcaacga actttacttc cgccggctgg aaacccctgc cgttatcgaa gaaggtgaac
     5401 gccggcttca agccaaagcc gcgcgcctca aagaaatcgc cgccgcacaa aaccccgacg
     5461 atccgccctt cctgatttcc gacttcggca cgcgccgccg ctacaagctc gcgtggcagg
     5521 aacacgtcat ccgcaccctg ctcgaagccg ccccaagcat cgtgcgcggc accagcaatg
     5581 tctttctcgc caaaaaactc ggcattaccc ccatcggcac catggcgcac gaattcctcc
     5641 aagccttcca ggccctcgac gtacgcctgc ggaatttcca aaaggccgcg ctcgaaagct
     5701 gggtgcacga ataccggggc gatttgggcg ttgccctgac cgacgttgtc ggtatggatg
     5761 ccttcctgcg cgatttcgac ctctatttcg ccaaactttt cgacgggctg cgccacgaca
     5821 gcggcgaccc ttacgtttgg ggcgacaaag cctacgccca ctatcaaaag ctcaaaatcg
     5881 acagccgcac caaaatgctg accttctccg acgggctgga catcgaacgc tcttgggcat
     5941 tgcaccaata tttcaaagac cgcttcaaaa ccggcttcgg catcggcacc aacctcacca
     6001 acgatatggg gcatacgccc ttgaatatcg tcttgaaact ggtcgaatgc aacgggcagt
     6061 ccgtcgccaa gctgtccgac tctccgggca aaaccatgac caacaacagc accttcctcg
     6121 cctacctgcg ccaagtgttc gacgtacccg aacccgaaac gccgtaaacc ggcagaaaaa
     6181 gcgcacaatt cctgtttctg ccgcataaaa tcttttaaaa taccgcctga tttgaattta
     6241 accgaaagac cgaacttcat gaacctacat caaaccgtcg aacacgaagc cgccgccgcc
     6301 tttgccgccg caggcatcgc cgacagtccc gttgttttgc agccgaccaa aaatgccgaa
     6361 cacggcgatt tccaaatcaa cggcgtgatg ggtgcggcga aaaaagccaa acaaaacccg
     6421 cgcgaactgg cgcaaaaggt cgccgacgca ttggcaggca acgccgtcat cgaaagcgcg
     6481 gaagtcgccg gcccgggctt catcaacctg cgcctgcgcc ccgaatttct cgcgcaaaac
     6541 attcagacgg ccttgaacga cgcgcgtttc ggcgtggcaa aaaccgacaa accgcaaacc
     6601 gtcgttatcg actattcttc gcccaatctg gcaaaagaaa tgcacgtcgg ccacctgcgt
     6661 tccagcatta tcggcgacag catttcgcgc gtgttggcat ttatgggcaa caccgtcatc
     6721 cgtcaaaacc acgtcggcga ctggggtacc cagtttggca tgttggtcgc ttatttggtc
     6781 gagcagcaaa aagacaatgc cgcgtttgaa ctggcggatt tggagcagtt ttaccgcgcc
     6841 gccaaagtgc gctttgacga agatgccgcc tttgccgaca ccgcgcgtga atacgttgtg
     6901 aagctgcaag gcggcgatga aaccgtgtta gcgttgtgga aacagtttgt tgatatttcg
     6961 ctctcgcacg cccaagccgt ttatgacacg ctgggcttga aactgcgccc cgaagatgtg
     7021 gcgggcgaat cgaaatacaa cgacgatttg cagcctgtgg tcgatgattt ggttcaaaaa
     7081 ggtctggcgg tcgaagacga cagcgcgaaa gtcgtgttct tggacgagtt taaaaacaaa
     7141 gaaggcgaac ccgccgcatt tatcgtacaa aaacaaggcg gcggcttcct ttacgcctcc
     7201 accgatttgg cgtgtctgcg ctaccgcgtc ggcacgcttc atgccgaccg cctgctgtac
     7261 gtcgtcgacc accgtcaagc cctgcacttt gagcaactct ttaccacttc ccgcaaagca
     7321 ggctatctgc cggaaaacgt cggcgcagca tttgtcggct tcggcacgat gatgggcaaa
     7381 gacggcaaac cgttcaaaac acgcagcggc gacactgtaa aactggtcga tttgctgacc
     7441 gaagccgtcg aacgcgccac cgctttggtg aaagagaaaa atcccgaatt gggcgcggac
     7501 gaagccgcca aaatcggcaa aaccgtcggc atcggcgcgg tcaaatacgc cgacttgagc
     7561 aaaaaccgca ccagcgatta tgtgttcgac tgggacgcca tgctctcgtt tgaaggcaac
     7621 accgccccct atctgcaata cgcctacacc cgcgtgcaaa gcgtgttccg caaagcaggc
     7681 gaatgggatg caaatgcgcc aaccgttttg accgaaccgc tggaaaaaca gcttgccgcc
     7741 gagctgctga aatttgaaga cgtactgcaa agcgtggcgg acacggcgta tccgcactac
     7801 ctcgccgcct acctctatca aattgcgacc ctgttcagcc gcttctacga agcctgtccg
     7861 atactcaaag ccgaaggcgc aagccgcaac agccgcctgc aactggcaaa actcaccggc
     7921 gacacgctga aacaaggctt ggatttgctg ggcatcgatg tgttggacgt aatgtaaaac
     7981 cgcaccgccc gattgcggac aacagcctcg ccatccttat ccgaatctga aaaaagcggc
     8041 gcgatacacc gtatccgccg cccctcccaa aatgcgaaac aaacaaacgc caagcaagca
     8101 agcaagcaag caaaaaatta taaccccctt cctgccgacg cacgcacttt ccgcgcggcg
     8161 cattcccctt tttccgcccc tcaaacccgc cttttcttca ggcagggttt cagcccgccc
     8221 cctttccctg ttttcctttc cccgacacgc gtgcgcgacc cctgccgcat cgtgccgcac
     8281 tttcgcgccc ggacggcatc gttccgccat ccggttctct gtttcaaata cccctgtttc
     8341 agaaagaaat tctagatgct tcaacacaca ggacgacata taaagcgtcg ccctatatgt
     8401 tgccctgatt tggaaggggt tacgcctccc aaataaagtc tgatcctgcc gccccgaagg
     8461 acagatgtcc gagtggcgaa gtttcaaccg aaaaggaaat acgatgaata ttcacaccct
     8521 gctctccaaa caatggacgc tgccgccatt cctgccgaaa cggctgctgc tgtccctgct
     8581 gatactgctt gcccccaatg cggtgttttg ggttttggca ctgctgaccg ccaccgcccg
     8641 cccgattgtc aatttggact atcttcccgc cgcgctgctg atcgccctgc cttggcgttt
     8701 cgtcaaaatt gccggcgtat tggcgttttg gctggcggtt ttgtttgacg ggctgatgat
     8761 ggtgatccag cttttccctt ttatggacct catcggcgcc atcaacctcg ttcccttcat
     8821 cctgaccgcc cccacccctt atcagataat gaccgggctg ttgctgctgt atatgctggc
     8881 gatgccgttt gtgttgcaga aagccgccgc caaaaccgac ttccggcaca ttgccgtctg
     8941 cgccgccgtt gtggcggcag ccggctattt caccggccat ttgagttact acgaccgggg
     9001 gccgatggcc aatatcttcg gcgcaaacaa cttctattac gccaaaagtc aggcgatgct
     9061 ctacaccgtc agccagaatg ccgactttat taccgccggc ctggtcgatc ccgtcttcct
     9121 ccccttgggc aatcaacagc gtgccgccac gcatctgaac gagccgaaat ctcaaaaaat
     9181 cctctttatc gtcgccgaat cttgggggct gccggccaat cccgaacttc aaaacgccac
     9241 ttttgccaaa ctgctggcgc aaaaagaccg tttttcggtt tgggaaagcg gcagttttcc
     9301 ctttatcggc gcgacggtcg aaggcgaaat gcgcgaactg tgtgcctacg gcggtttgcg
     9361 cgggttcgca ctgcgccgcg cgcccgacga aaaatttgcc cgctgcctcc ccaaccgttt
     9421 gaaacaagaa ggttacgcca cctttgcgat gcacggcgcg ggcagttcgc tttacgaccg
     9481 cttcagctgg tatccgaggg cgggctttca agaaatcaaa accgccgaaa acctgatcgg
     9541 taaaaaaacc tgcgccattt tcggcggcgt gtgcgacagc gagctgttcg gcgaagtgtc
     9601 ggcatttttc aaaaaacaca gcaaaggatt gttttactgg atgacgctga ccagccacgc
     9661 cgactatccc gaatccgaca ttttcaacca caggctcaaa tgcaccgaat acggcctgcc
     9721 cgccgaaacc gacctctgcc gcaacttcag cctgcacacc caattcttcg accaactggc
     9781 ggatttgatc caacgccccg aaatgaaagg cacggaagtc atcatcgtcg gcgaccatcc
     9841 gccgcccgtc ggcaacctca atgaaacctt ccgctacctc aaacaggggc acgtcgcctg
     9901 gctgcacttc aaaatcaaat aacaacaatg ccgtctgaac gcaccaacag ccttcagacg
     9961 gcattttgca gacagaccga cccatcaagc ccactttttt catcatctcc gataaatt
//
LOCUS       DAXHRI010000061         9910 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXHRI010000061 DAXHRI010000000
VERSION     DAXHRI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.061"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(14..184)
                     /locus_tag="ACFM75_001122"
     CDS             complement(14..184)
                     /locus_tag="ACFM75_001122"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6255679.1"
                     /translation="MRIGNDLFVKRVQRIPGKLLVTSENPRYAPFEIDLSNTQDDIAI
                     IGRVEWYGRSID"
     gene            complement(393..1292)
                     /locus_tag="ACFM75_001123"
     CDS             complement(393..1292)
                     /locus_tag="ACFM75_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFC6255680.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCDVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            1340..1591
                     /locus_tag="ACFM75_001124"
     CDS             1340..1591
                     /locus_tag="ACFM75_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6255681.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            1593..3569
                     /locus_tag="ACFM75_001125"
     CDS             1593..3569
                     /locus_tag="ACFM75_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC6255682.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRSAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYEMFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVAKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAPDMLSA
                     EELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLDDASAVNVFDMD
                     GKFITKAQADGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNVIEAEYAVLPKTGTDDFVLFETDRS"
     gene            3665..4519
                     /locus_tag="ACFM75_001126"
     CDS             3665..4519
                     /locus_tag="ACFM75_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFC6255683.1"
                     /translation="MNQTQLARGIGTSPASISMYLNGTYAEKGGNYETIEPKIEAFLE
                     MQDSKAQREELVLGFVSTKTTRRIAEVMRDAHEGGETVVIYGQAGLGKTQAVKNYCEK
                     NPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLFESVSDRLRDSGRLIVVDEAE
                     NLPLRALEIVRRLHDETGCGLVLSGMPRLVANLRGKHGELVQLYSRVSVALNLGESLP
                     DDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLMRGAVRTANKNGIKMQVGIVK
                     KYSSLIIR"
     gene            4610..4876
                     /locus_tag="ACFM75_001127"
     CDS             4610..4876
                     /locus_tag="ACFM75_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255684.1"
                     /translation="MQVLKKVDWKMFVARSFWRCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKELTKVEKMERQADLEVKVWEEQYESMSVEEKMKGIVYE"
     gene            4985..5158
                     /locus_tag="ACFM75_001128"
     CDS             4985..5158
                     /locus_tag="ACFM75_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255685.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTGGNKDE"
     gene            5151..5318
                     /locus_tag="ACFM75_001129"
     CDS             5151..5318
                     /locus_tag="ACFM75_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255686.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            5382..5609
                     /locus_tag="ACFM75_001130"
     CDS             5382..5609
                     /locus_tag="ACFM75_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255687.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            5624..6145
                     /locus_tag="ACFM75_001131"
     CDS             5624..6145
                     /locus_tag="ACFM75_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFC6255688.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGL
                     EDFVIEPTEQELA"
     gene            6145..6336
                     /locus_tag="ACFM75_001132"
     CDS             6145..6336
                     /locus_tag="ACFM75_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255689.1"
                     /translation="MAKIVITIKDEMPVNGLNGVTISYDGDLEPQGELTMAQMTAYNI
                     KKLMDAVEFETAKRLSKAN"
     gene            6381..7268
                     /locus_tag="ACFM75_001133"
     CDS             6381..7268
                     /locus_tag="ACFM75_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6255690.1"
                     /translation="MWFKQVTPFRLLELPEKRYLDESLGNSWFTEPQGLDWFSEGFTP
                     PNAFTDLAVFEAQKTMLISLMREEKVLPSAAIKHKLDEQIIKIQAHEGRNVGRKEKQE
                     LREAIIDDLLPKSLIKSSRTYGLFAGEWLFVDTANRRKAENLLTKLREALGGLPAQQP
                     LTRQSPSALMTNWLLRGEAQGLFVLDSDVTLVGAGDVAPKVKISRKDLTAEDVVQHAK
                     NGMTVTELGLVWNGRVAFILTQDLTLKRIQWLDVVQEEAEGSCDDAKSMSYATQLLME
                     AALSAILGELVDLLGGWQE"
     gene            7467..7868
                     /locus_tag="ACFM75_001134"
     CDS             7467..7868
                     /locus_tag="ACFM75_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC6255691.1"
                     /translation="MRRALIAKIKIAQKELGLDDATYRAVLERVTGKRSCTECSIPEL
                     ERVVEDLRQHGFTPKKTAGRRPNRRSSADPMMRKIEALLLDNGWTWNYAHGTAKKMFK
                     VDRVEWLSDGNMHKLVAALQIAANRRKKGAV"
     gene            7868..8308
                     /locus_tag="ACFM75_001135"
     CDS             7868..8308
                     /locus_tag="ACFM75_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6255692.1"
                     /translation="MYETADFGAVKHLLPDSVQALITVIGFNETLELVRLMGGTTYPL
                     RQGYTKNSQSRVAYLEEIIGSEAAGRLVEAMAPCNLFIPRCETALYELRNRKIRSQFD
                     RQTAGGTPAYEAVNDLALAHRLSDRHVWRILKQADKEAEQENLF"
     gene            8310..8831
                     /locus_tag="ACFM75_001136"
     CDS             8310..8831
                     /locus_tag="ACFM75_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255693.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            8974..9519
                     /locus_tag="ACFM75_001137"
     CDS             8974..9519
                     /locus_tag="ACFM75_001137"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6255694.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNNY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            9516..9722
                     /locus_tag="ACFM75_001138"
     CDS             9516..9722
                     /locus_tag="ACFM75_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255695.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            9725..9889
                     /locus_tag="ACFM75_001139"
     CDS             9725..9889
                     /locus_tag="ACFM75_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255696.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
BASE COUNT         2759 a         1993 c         2769 g         2389 t
ORIGIN      
        1 ttttaaaatg gatttaatca atactccggc cataccactc aacacggcct atgatggcga
       61 tgtcgtcttg ggtattactc aaatctattt caaacggtgc gtaacgtgga ttttcagacg
      121 ttacaagcag tttgcccggt atacgttgca cacgtttgac aaagaggtca ttgcctatac
      181 gcaagacata taggccgtcg cgcggctcgg tttcggcgtg gttgattaga atgtttataa
      241 aaggctttaa ctagaaactg aacatggaac ttgtataata gttccatgtt tgaaaaaatc
      301 ttttaatatc atacatctaa aaaaacaaag catctgaaga ccataagcta aacatgaaac
      361 ttgaatttaa aggcggagtt tcatgtttaa gcctattttt tccgtttttt agtcgatact
      421 gctgcatatg ttttttgcaa atcttcatca cttaattcag tcaggctttc tactgagaaa
      481 tttttttcga ggtaactttt caaccaatct tcaagctcaa gctgttttac atttaatttg
      541 atataagcgt atttcttttt tcgccagtcg ggatcgttgt tctttgatgt tgctgtattg
      601 ctcaacctac ccagccattt tctgagataa gtttctgctt tgtcgtaatc gctcaatgcg
      661 attagtttgt aacttggaac tttacagtga gcattcagac tagcccaaat tgcacggatt
      721 gattttggcg accgtttggc aatctcttct gctgcggcaa cttgtttaac aagttgttgc
      781 agctttgaag cttgctcaag cgttatatgt tccttgccgg gttttgaatc gacacgggtt
      841 ctgatgacgt gattttgggt attaatttga tgaacaaccg aaccagatga ggcgataaca
      901 gcgttactga cgttgccgtt aatctgattc tcaacgtttg atttgccaat agcgtcgtcc
      961 aattttttat taaggtcgtc tgaaacagca tttaaatttg ccgaatgaat gcctgtaaag
     1021 acaaattgaa tatccacccc taattggaca gcagcaagta aaaagtccgc aggaatgttg
     1081 acctgcccgc tttcatacag tctcaaactt tctgcactgc atccagtttg ctcagcaaaa
     1141 ttctttcgag agtatccaag tcggcttctc tcttcaacta aattcaatgc taaatcaatg
     1201 cgttccataa aaacatcgca aaatattacc aaatatattt ggtataccaa atattttgtg
     1261 tatatcatcc aagtaacagt taaggcaggc attaaagcct gtaaggtaag taagtaatgg
     1321 attctatcat ggaggcatta tgcaagcgaa cgaaatctat aaatccttag aagaaaaaaa
     1381 tatatcggca agaatgttgg cttcggcttt aggggtaact aaccaatctg tttctgaggt
     1441 cataagaaat ggacgaggaa gcaaaagaat tgcggaagca atcgcgaaag tgattcaaaa
     1501 agatttagtt gaagtttttc cgcattacaa gaaaacagat tgtcgagaca aaaagatagc
     1561 tgaattgaaa gagatgctcg gagtgggttg aaatggaata tatggatctc aaaagcctgc
     1621 ttaattttgg gttgccaaat atgccaagaa cagtaatagg gctgagaaaa aaagcgcaaa
     1681 gggatggttg gcaaacgaga aggcgtcaag gtaagggtgg tggtgttgaa tatgccctcc
     1741 ctgccgaaat ccgagcagcc atcatgaaac ggcagtcgga cgagctggcg gagaagatgc
     1801 cgaaaaccct gccccaagtc agaccgggga cggcgatgtc ggctcaagca ctggctgaag
     1861 cggccaagct gttgaacgag aaacaacggt cggtggcgga tgcgcgatgt gcggtggtgg
     1921 cggcggtgtt ggggattaaa taccaatacg attgctctgc caaggctgcg gtggctcagt
     1981 ttttgggctt gctggcagaa ggtaaattgg acgcggtcac gcttgggaac ttggaaaagg
     2041 ccaatgaccg cagccggtcg gcgaaggttg gcgaacgtac tttagacggc tggatttctg
     2101 cttatttgaa agcggaaaac gcgacggagc ggttggttgc tttggctccg aagacgacga
     2161 aggcggtcaa gccgattgaa agttacggat ggttgccgat gtttatgcag tttcacaata
     2221 ttccgtccgc gccaaagctg gcgcacagct accgccggtt tgtgcagtgg gcggaagcgg
     2281 aaaatatgcc ggtcaatgat gtgcctaatt tgagtatggt gcggcgcgtt tgggacaagc
     2341 tcccgttgat tatgcaggag cgcggcagga aaacgggggc ggcttataaa tcgctgctgc
     2401 cttatgtgaa acgtgattgg ggggctttga agcctaacga tgtttggatt ggcgacggcc
     2461 acagctttaa agcgaaggtg gcacatccgg tacacggcag gccgtttaag ccggaagtga
     2521 cggtgattat tgatggttgt acgcggtttg tggtgggttt ttcggtgtcg ttggctgaaa
     2581 gttgtgtggc ggtatcggac gctctgcgta tcggggtcaa gcactttggt ttgccgatta
     2641 tctactactc ggataacggt ggcggccaaa ctggtaagac gatagatcat gaaatcacgg
     2701 gtattacgtc ccgattgggt atccggcatg aaacgggtat cgcaggcaac ccgcaaggcc
     2761 gaggcatcat tgagcgatgg tggaaagaca atctgattga gatggcgcgc cagtatgaga
     2821 tgtttgcggg cgcaggaatg gacagcagca cgaagaacct gatgtaccgc aagatggaaa
     2881 gtgcgtttaa tgctttggaa aaaggcaagg atttgacgga ggagcaacag aaatatttga
     2941 aaaaactgcc gagctggtcg cgctttatcg cggatgtggc caagtgtatc gacgaataca
     3001 acaaccgccc gcacggcgag ctgccccgac atccggacgg cgggcattat tcgccgaagg
     3061 cttatcggga aatgaggctg gaacaggacg ggattgcgcc ggatatgttg tcggcggaag
     3121 agctggcgac gatgtttatg ccgcaggaag tgcgaaaggt acagcgcggt tggctggatt
     3181 tgttcaacaa ctcttatttc tcaaccgagc tggcggagta tcacaaggac gaggtacggg
     3241 tcagctacga tttggacgat gcttcggcgg tcaatgtgtt tgatatggac ggcaagttta
     3301 tcactaaggc tcaggccgac ggcaataccc gcgaggcttt cccgacggct cgtatcgacc
     3361 aactggcgga aaaacgccga aaaggcaaaa tcaagcgggc ggaaaatgca atcaagctcg
     3421 caaatgcgga agtcaatccg gctttggaac aggctgcggt ttgggacgag ctgggacatt
     3481 tgggcggaaa cgtcatcgag gcggagtatg cggtattacc gaaaacgggc acggacgatt
     3541 ttgtgctatt tgagacggat agaagttaaa acggttttaa acctctttta aaaggactaa
     3601 aaaaatgaaa caaattaatc aagcattgca acaaaaactg gcttaattta aagccaaatc
     3661 aggtatgaac caaacccaac tggcacgcgg tatcggtact tcgccggcat ccatcagtat
     3721 gtatctgaac ggcacttatg cggaaaaagg cggtaattat gaaaccatcg agccgaaaat
     3781 cgaggcgttt ttggagatgc aggacagtaa agcgcaacgt gaagagctgg tgttgggatt
     3841 tgtatcgact aagacgacac gccgtattgc agaagtgatg cgcgatgcgc acgaaggcgg
     3901 cgaaacggtg gtgatctacg gtcaggctgg cttgggcaag acgcaggcgg tcaaaaacta
     3961 ctgcgagaaa aaccctgcgg ccatcctgat tgaggctaat ccgagcttta cggctttggt
     4021 cttgatgcgc aagttggcga cagcggcgaa ggtatcggcg atgggcagcc tgaatgattt
     4081 gtttgagtct gtatctgacc gcctgcgcga ttcgggccgt ctgattgtgg tcgatgaagc
     4141 ggaaaacctg ccgttacgcg cccttgaaat tgtacgccgt ctgcacgatg agactggctg
     4201 cggcttggtg ttgagcggta tgccccgact ggttgcgaat ttgcgcggta agcatggcga
     4261 gctggtgcaa ctttacagcc gcgtgtcggt tgcgctgaat ttgggcgaat ctttgccgga
     4321 tgacgaactc tttgagattg cgaaagcggc tttgcctgat gcggacgaag aaacgctctt
     4381 ggaactggtt aaacatagca acggcaatac gcgccggatg agcaaattga tgcgcggcgc
     4441 ggtacgcacg gcgaacaaga acggtatcaa gatgcaggtc ggtatcgtta agaaatacag
     4501 ctccctgatt atccgatagg gccgtctgaa acggtaagtc tctgacaggg ctatatattt
     4561 tttaccctat gattttaata acttattgtt ttctaaagga aaacgcaaaa tgcaagtttt
     4621 gaagaaagtt gattggaaga tgtttgtggc acgctctttt tggcggtgtg tgccggttgg
     4681 tttgacggcg ggcgtgtggt gttttgtggc ggggatggcg ttgcattcct gcgcgaaaga
     4741 acccgaaccg gttgcgaaag agctgacgaa ggtcgagaag atggaaagac aggcggattt
     4801 ggaggtcaag gtatgggaag agcaatacga gtcaatgagc gtcgaggaaa aaatgaaagg
     4861 gattgtttat gagtgataag ccaattttat tgaaccctgc ggcgaaaaaa gaggctttgg
     4921 atcgggcggt aaaggaaatc cgtgcgaaat atggcgataa ggcgattgtg aaaggatgtg
     4981 tgaaatgagt ttcggacgac gtaatacgga ttggcaggct tggggacaac accgcaggcg
     5041 tgcgacggcg cgaatggcgc aaaaaagccg ggagcgcgaa atcgaggaat atcaggcgca
     5101 gtttgaagat aaggacggca aaggtcgtct gaaaacggga ggaaacaaag atgaatgaaa
     5161 aagatttgat cgaatggctc gaagaccgtg gcgagctgat ggtcatgaag aaggacggcg
     5221 aaggctttgt gattgcagcg cgtgcgccgg acgggatgtg gaagactgcc gaggcggaaa
     5281 ctttggctca ggcgataact ttatgggagg aagtgtgatg tttaagtttt tgagaaaccg
     5341 cgcgaaagtt gaagcaattt taaacaagaa aggaaagtga aatgaaagtg tatgttttta
     5401 aaattagtaa tgaaaacggt aagttaaaga tagaactccc cgaaatccca atgggtaagc
     5461 aaattgacga agttgatttg attgctgggc taaccacgga atttattgca agtatgttac
     5521 gtgatgccca aaaagatcgt cgcaaattcg taatcgacgc atcaaatcaa ttggctgcaa
     5581 tccagacata tcaaaaaatc ttcaattaag gaaggaagag aaaatggcta aaacccgaat
     5641 caaacagccc gccatcgaag cggcacaaga caaaacggaa gttactgcat ttatccgcca
     5701 aatcggcgac ttgcagcgcg aagtcaaacg cctggaaacc gaagccggag acaaaaaagc
     5761 ggctatcgaa gaagaatatg ccgccaaagc cgcgccgatg tgtgccgaaa tcatgagcct
     5821 gaccgaacgt gtggccgcct actgcgaagc gcataaggac gagctgacgg aaaatggtaa
     5881 aaccaaaacc gtggacttta ctactggcct gattaaatgg cgcattcgtc cgccatccgt
     5941 caaggtaacg ggcgtggcgg ccgtcttggc gtggctctcg gaaaaatccg cctttgccga
     6001 gtttgtccgc actaaaaagg aaatcgacaa agacgccatc ctgaatcaaa aagagcgttt
     6061 ttcagacggc caagttccag gaattaagat tgtatcgggg cttgaggatt ttgtgattga
     6121 gcctactgag caggagttgg cgtgatggcg aaaattgtta ttacgataaa agacgagatg
     6181 ccagtaaacg gcttgaacgg cgtgaccatc agttatgacg gcgatttgga gccgcaaggc
     6241 gaactgacga tggcgcagat gacggcttat aacatcaaga aattgatgga tgcggttgag
     6301 tttgagaccg caaaaaggct gagtaaagca aattgaccca cggcgggaac aacccgccat
     6361 ttttgaaaaa aggattagat atgtggttta aacaagttac cccattccgt ttactggaat
     6421 tacctgaaaa acgctattta gatgaatctc ttggaaatag ttggtttacc gaaccacagg
     6481 gcttggactg gttttccgag ggctttactc ccccgaatgc gtttacagac ctagctgtgt
     6541 ttgaagctca aaaaactatg cttatcagtt tgatgcgaga agaaaaagta ttacccagtg
     6601 cggccatcaa acataaattg gacgaacaga ttattaaaat ccaagcccat gaaggacgaa
     6661 atgttggtcg taaagaaaag caggaattac gcgaagcaat tatcgacgac ctgctgccta
     6721 agtcgttgat taaaagcagc cgcacttatg gtttatttgc tggagagtgg ttattcgttg
     6781 atacggcaaa tcgccgcaag gccgaaaacc tgttgaccaa gttgcgcgaa gcccttggcg
     6841 gcctgcctgc tcaacagcct ctcacccgtc aatcgccgtc agcattgatg accaactggc
     6901 tgttgcgggg cgaagcccaa ggcctgtttg tgttggatag tgatgttacc ctggtcggcg
     6961 cgggcgatgt tgctccaaaa gttaaaatca gccgcaaaga ccttaccgcc gaagatgtgg
     7021 tacaacacgc caaaaacggt atgactgtaa ccgaactggg cttggtctgg aatggccgcg
     7081 tggcatttat cctgacacag gatttaacac tgaaacgtat ccaatggctg gacgttgtac
     7141 aggaagaagc tgaaggcagc tgtgatgatg cgaaaagtat gtcttatgcc acgcagctac
     7201 tgatggaggc cgcactgagt gcgattcttg gtgagttggt ggatttactg ggaggttggc
     7261 aggaatgatg gagggttggg atggattctg aaggctggga tttttaaagc ttgattaaag
     7321 gccgtctgaa atggggttta aaacctgttt caggcggcct tttttatgtc tgtcagtttc
     7381 gcaaaaaaca tcgacttaat actatatatt gtattttatt ggtataatat gtgctaattt
     7441 ataatatatg ttgtattgga gagataatgc gccgggcgtt gattgcgaaa attaaaatcg
     7501 ctcaaaagga gctgggtttg gatgatgcga cgtatcgcgc ggtgttggag cgtgtgacgg
     7561 gcaagcgttc gtgtaccgag tgcagcatcc ctgagctgga gcgcgtggtc gaggatttgc
     7621 gccagcatgg gtttacgccg aaaaaaacgg caggccgtcg cccgaaccgc cgaagctctg
     7681 ccgatcctat gatgcggaaa atcgaagccc tgctgctgga taacggctgg acttggaatt
     7741 atgcgcatgg tacggcgaaa aagatgttta aggttgaccg cgtggaatgg ttgtccgacg
     7801 gcaatatgca caagctggtg gcagcattgc agattgcggc gaaccgccgt aagaaagggg
     7861 ctgtgtgatg tatgagacgg cagattttgg cgcggtcaag catctgctcc ctgatagtgt
     7921 acaggcgttg attacggtca tcgggtttaa tgaaacgctg gagctggtgc gcctgatggg
     7981 cggtacgact tatcctttgc ggcagggtta tacgaaaaac agtcaatccc gtgttgcata
     8041 cttggaagag attatcggca gtgaggcggc cggtcggctg gtggaggcaa tggctccgtg
     8101 caatctgttt ataccccgtt gcgagacggc cttgtatgag ttgcgaaacc gtaaaatccg
     8161 cagtcagttt gaccggcaga cggcaggcgg tacccctgct tatgaggccg ttaacgattt
     8221 ggccttggca caccgcctaa gcgaccgcca tgtgtggcga attttaaagc aggcggataa
     8281 ggaagcggag caggagaatt tgttttagaa tggaatgcca tgcagatgta tggcatttta
     8341 ttttggagaa aaatatgaaa aagttttatt ttgtgctgct ggcgttgggt ttggcagcgt
     8401 gtgggcaaga acaatcgcag aaagctgatg cggagcagta tttttttgcc aataaatatc
     8461 aatttgcaga tgagaaacag gctttttatt ttgaacgcgc cgcccgtttc cgtgtattgc
     8521 aacaaggcct tggcggggat tttgagaggt ttttaaaagg agaaatacct aatcaagaaa
     8581 atcttgcaaa gtatcgtgaa aatattactc aagcagtcgc ttattatgcg gacacgaatg
     8641 gagatgatga cccataccgc gtctgcaaac aggctgcgca agatgcagaa atcctgatga
     8701 agagtatggt aacaagcggt ggaggcggta caactgattt agataaggaa agttatcaaa
     8761 attaccgaaa atcaatgcaa gaatgccgta aaacaataac ggaagctgaa gccaatttgc
     8821 cgaaaaaata aaataaacga ttctaaggcc gtctgaacaa caggcggctt ttttgttgcc
     8881 tactgacact gtttcgcccg ctgcaaaagc catgccgttt gaaaatgtaa gcctctgaaa
     8941 gtgcatttta atctgatttt gaggaaggct ttaatgagca aaattatttg tctgactgcc
     9001 ggacacagca acaccgaccc gggtgcggtc aacggcagcg accgtgaggc ggacttggcg
     9061 caggatatgc gcaacattgt ggcttcaatc ctgcgtaaca attacggcct gaccgttaaa
     9121 accgacggca cgggcaaagg caatatgccg ctgcgcgaag cggtcaagct gattcgcggc
     9181 tcggatgtgg cgattgagtt tcacaccaac gctgccgtca gcaaagcggc gacaggcatc
     9241 gaagccttga gtaccgttaa aaacaaacgc tggtgtcagg tgttgagcaa agccgttgcc
     9301 aagaaaaccg gctggaaact gcgcggcgaa gacggcttta aacccgacaa tgcgggccag
     9361 cattcgcgcc tggcttatgc acaagccggc ggcattgtgt ttgagccttt tttcatcagc
     9421 aacgacactg atttggcctt gtttaagacg actaaatggg gcatctgccg cgcgattgcg
     9481 gacgcgattg cgatggaatt gggggcggca agagtatgaa tattattggt aaattgaaag
     9541 aagctgcttc ctattttctt acaaaattga ttggagaaaa tcctagtaat gagcaggtaa
     9601 accgcgcact tatacagatg ccaaatgttc gtccgataca cacctatcca cgcccaaatt
     9661 taagaaactc aggcgtggca gccgcgaaac gcgcggcgcg caaacgcaag aatcgtcgtt
     9721 aatcatggga caggttgcgt tttacgaaaa gatgattggg ctgtggtcgg ccaaaagccg
     9781 tgaggcaagc gaacaggcag acttggcggc gtttgaattt gcggagggcg aactggccaa
     9841 ttatcaggaa atgctgaaac ggcacctgca aaccaaaagt gtggaatagc aatgcgtatt
     9901 ttggatattt
//
LOCUS       DAXHRI010000062         9802 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXHRI010000062 DAXHRI010000000
VERSION     DAXHRI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.062"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(312..545)
                     /locus_tag="ACFM75_001140"
     CDS             complement(312..545)
                     /locus_tag="ACFM75_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC6255697.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(563..697)
                     /locus_tag="ACFM75_001141"
     CDS             complement(563..697)
                     /locus_tag="ACFM75_001141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255698.1"
                     /translation="MPSELFRRHFYCFWQDLSIIFLENIVFSQIDHDSAASKALACAN
                     "
     gene            complement(711..911)
                     /locus_tag="ACFM75_001142"
     CDS             complement(711..911)
                     /locus_tag="ACFM75_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC6255699.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="ACFM75_001143"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="ACFM75_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC6255700.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGSVDLDRG
                     CITALGKGDKQRMIPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="ACFM75_001144"
     CDS             complement(2010..2450)
                     /locus_tag="ACFM75_001144"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC6255701.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2771..3709
                     /locus_tag="ACFM75_001145"
     CDS             2771..3709
                     /locus_tag="ACFM75_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC6255702.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYSSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3838..4131
                     /gene="hfq"
                     /locus_tag="ACFM75_001146"
     CDS             3838..4131
                     /gene="hfq"
                     /locus_tag="ACFM75_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC6255703.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4216..4782
                     /locus_tag="ACFM75_001147"
     CDS             4216..4782
                     /locus_tag="ACFM75_001147"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6255704.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPSLCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            4836..5210
                     /locus_tag="ACFM75_001148"
     CDS             4836..5210
                     /locus_tag="ACFM75_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC6255705.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5220..5714
                     /gene="folK"
                     /locus_tag="ACFM75_001149"
     CDS             5220..5714
                     /gene="folK"
                     /locus_tag="ACFM75_001149"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC6255706.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKQLGNQGIRL
                     LPDR"
     gene            complement(5842..6321)
                     /locus_tag="ACFM75_001150"
     CDS             complement(5842..6321)
                     /locus_tag="ACFM75_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC6255707.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKM
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(6541..7278)
                     /gene="ubiE"
                     /locus_tag="ACFM75_001151"
     CDS             complement(6541..7278)
                     /gene="ubiE"
                     /locus_tag="ACFM75_001151"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC6255708.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPFEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7321..7689)
                     /locus_tag="ACFM75_001152"
     CDS             complement(7321..7689)
                     /locus_tag="ACFM75_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC6255709.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7809..9428
                     /gene="mnmC"
                     /locus_tag="ACFM75_001153"
     CDS             7809..9428
                     /gene="mnmC"
                     /locus_tag="ACFM75_001153"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC6255710.1"
                     /translation="MDNLVWDGIPDIRTLDQAIRKHAPPLNLIICLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILHDIRFWLVPPSRTCRLHEHFHHISWQ
                     TEAISQTESKSDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLRHILPESETWGGNGIIH
                     LNYSRIEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLRHPLIGLHEDTPLTDISYDGEKWIASTPNGIFSATHIIYCTGANSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNDAEETSNRQALAHLNPALAESLFAANPIPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAVAAEILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9542..9659)
                     /locus_tag="ACFM75_001154"
                     /pseudo
     CDS             complement(9542..9659)
                     /locus_tag="ACFM75_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         2628 a         2980 c         1960 g         2234 t
ORIGIN      
        1 taccgattac acaaacacat atcatcttta cacaatcatg cttccatcaa cagtaaaaca
       61 tgatatgatt gccaacaata acatctcaca ataaattttc taatttttat tgaaaaaatc
      121 aataaataag aattctccca accgacaaat aaaataaaga aagggttaat atgcaacatc
      181 gaagaagact agcaatttac caagcatcca aacgtgcttc ctttaccggc aggtcatccg
      241 ccccgcaaaa acgtaaagaa cgttgatttg aaaaaaatgc cgcctgaagt cctgcttcag
      301 acggcatttt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
      361 cagatttcct catctcccgc caacaaaacg ggaaccagct cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatt ttgaaaaggt
      481 ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt cgaggaagat gatactcaag tcctgccaaa
      661 aacagtaaaa atgccgtctg aacagttcag acggcatttc gaaaaccgtt ttacgcttga
      721 acgttgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccgc agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat ggtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgca aatataaata aaaacggttc gcattgcaag gtcggcatac acggtttgtc
     1021 ttggtaattt tttatcaagt tttcatttcc gattgatttc ttattaaacc gaagcagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacgtt ggcaacatgg
     1141 gtataaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaagccgt gccgcaccag atgcgtggca aaggcgtggc gcaggctgtg cgggctgatg
     1261 tgcccgatgc ctgcctgact tgcatattct ttgacaatca tccatgccaa ctgacgggaa
     1321 atgcccgtct ttttctgact gacaaacaat gcgtcgcaat tcctgccttt cagcagaagt
     1381 gggcgtgcct ccgtataata gcgttccacc caatacgccg actcctgccc catcgggatc
     1441 atcctctgct tgtcgccctt gcccagcgcg gtaatacagc ccctgtccaa atccacactg
     1501 ccgaaattca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat caattcgagc
     1561 agtgccttgt cccgcaaacc gtgtggcgta tcagtatccg gagtggcaag cagccgggaa
     1621 atctgctgtt ctgtaatcag tgtcggaata ttcttgtcga ttttgggcgg tttcagcaaa
     1681 cgggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcac
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcatt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgac tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcagacggca tggttcacat tatcgggaaa gcgtttccaa tacttcctgc
     2041 gcgtgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 cccggcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacgacacgc agacgcaccg ccgcaatttc caaaccaacc
     2521 ccgattctac cgccccaaag gacagggatt caaccgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
     2641 ttataaaacc caatccccaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
     2701 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatat
     2761 ggaatcaaaa atgtccatcc atactctgaa acgcctgccc tcatcgctgc tgctcggtct
     2821 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
     2881 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacgataca
     2941 ccccaccaac acccaagcca ttacaggcgg tctgattctc tcctcacagt ctgccctggt
     3001 cgtcaacaac aaaaccggac agatactgta tcagaaaaac gccgacagga ttatgcccat
     3061 cgcctccatt tccaaactga tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
     3121 cgaaaccgtt accattacgc ccgacgaaat cgaccgcctc aaagggaccg gcagccgtct
     3181 tgccataggt acggcactta cacgcaaaga actgctgcac ctgagcctga tgagcagcga
     3241 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
     3301 cgccatgaac cgcaaagccc aaagcctggg catgtacagc agccgctttt acgaaccgac
     3361 cggactcaac ttccaaaacg tttccaccgc caaagacctg agccttatgg tcaacgccgc
     3421 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacagaccaa
     3481 aaacgggcag cagaactaca aaaactccaa tgcgctggtc agggaaggca tgtggaacat
     3541 cgaattgcag aaaaccggct acatacgcga agcaggcagg tctatggttg tcaaagccaa
     3601 cattcaaaat caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
     3661 caacgacgcc cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
     3721 ccggcggaaa accgcccaaa cacaaaaaaa attggtaatt tttcctattc aaggctacaa
     3781 ttcgacacgc acagggcatc tgcccgattt cacaatcata atagcggagt taaaaatatg
     3841 acagctaaag gacaaatgtt gcaagatccc ttcctgaacg ccctgcgtaa agagcatgtt
     3901 ccggtttcga tttacttagt taacggtatc aaattacaag gtcaggttga gtctttcgat
     3961 caatacgttg ttctcctgag aaacacttcc gtcacccaaa tggtttacaa acacgccatt
     4021 tccaccatcg taccggcacg ctccgtcaac ctacaacatg aaaacagacc ccaagccgca
     4081 ccgacttcga ccctcgtcca agtggaaacc gtccagcagc ctgccgaata atccgcacga
     4141 agcatgacgt gtcatatctt tcaatacctt accggacaac ggtaaggtat ttttattttc
     4201 agacagcatt taaaaatgtt attgcaaaac atccttccat tcgcccattg ccttttgcgg
     4261 aaggcacttc ccgaaggcgg caatgctttg gacggcaccg ccggcaacgg acacgacacc
     4321 cttttcctcg cacaaaccgc aggcatccgg gggaaagtgt gggcattcga catccagccg
     4381 caagccctga acaacacccg atgccgtctg caggaagcag gttacagcaa tgtacggctc
     4441 atcttggacg gacacgaaaa cctgaaacaa tatgtttcag aaccactaaa tgccgccatt
     4501 ttcaactttg gctggctgcc cggcggagat aaaagcctta ccacccatac ggaaaccagc
     4561 attccctccc tttgcgcaac cctctccctg ctgaaagaag gcggtatgct tattgccgtc
     4621 ctctatccgg gacacgaaaa cggcaaacag gaggcagaag caatcgaaca atgggcaaaa
     4681 aacctgcctc aagaacagtt tgccgttttg cgttacggct ttaccaaccg gaaaaacagc
     4741 ccgccctatc ttttggcatt tgaaaaactg cgtcaaaaat aactgtttgc ggtaaaataa
     4801 gccgattcta acccttattt tgatacgagc cgaccatgca gtatctgatt gtcaaataca
     4861 gccatcaaat cttcgttacc atcaccattt tggtattcaa catccgtttt ttcctacttt
     4921 ggaaaaatcc agaaaagccc ttggcgggct tttggaaggc actgccccac cttaacgaca
     4981 ccatgctgct gtttacggga ttgtggctga tgaaaattac ccatttctcc ccgttcaacg
     5041 cgccttggct cggtacaaaa atcctgcttc tgctcgccta tatcgcattg ggtatgatga
     5101 tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt ttacctgctc gccatgtgtt
     5161 gcgtcgcctg catcgtttac cttgccaaaa ccaaagtcct gcctttctga aacaccgtta
     5221 tgaacaacag acattttgcc gtcatcgccc tgggcagtaa tcttgaaaac cctgcccaac
     5281 aggtacgcgc cgcattggac acgctgtcgt cccatcctga catccgtctt aaacaggctt
     5341 cctcactgta tatgaccgcg cccgtcggtt acgacaatca gcccgatttc gtcaatgccg
     5401 tctgcaccgt ttccaccacc ttggacggca ttgccctgct tgccgaactc aaccgtatcg
     5461 aagccgattt cggacgcgaa cgcagcttcc gcaacgcgcc gcgcacattg gatttggaca
     5521 ttatcgactt tgacggcatc tccagcgacg acccccgcct caccctgccg catccgcgcg
     5581 cacacgaacg cagtttcgtc atacgccctt tggcagaaat cctccctgat tttattttgg
     5641 gaaaacacgg aaaggttgcc gaattgtcaa aacaactggg caatcaaggt atccgtcttt
     5701 taccggacag gtaattccgc acacagatgc cgtctgaatg ccggtttcag acggcatttt
     5761 taccctgccg ccaatctgta cgcaaaaaaa atcgcccctt gaaaaagggg cgcaaaagga
     5821 acacaaacca ctaccaaaac tttaaatctg caacactgcc tgccgcatac tgtatccgac
     5881 aggatataaa gccctcacca aatcgtttcg agaaatccaa acttcttcat cgccgacaga
     5941 aaacctgcct ttctccggca ccagctccaa caaaaacggc tgaaccgccg tatgcagctt
     6001 gtctttacac cgccccatct ttctagacag cgcagacagg gcgcgtttgt aggcattatc
     6061 cttgcagtca agctcccgcg cactgccgac ccagctcaaa cgaaggttgc ggctttcatc
     6121 ctcaatcagc aaaatgcctg ttttgacttc ccccacctcg ggctcgcatg aaagcgcaat
     6181 ataatatttg ccgtcgatgt tgacatacgc cgcttcatgc acctgacagt tgcagccgga
     6241 agtggcaagc agcgaataaa cagagtcccg atgcagtttc aaaatatcaa gggaaatctg
     6301 tttgaaaata ctttccggca tttttccctc ctttcgtctg ctgcgttgtt tactggattg
     6361 actgcattat acagacatct cccaatcttg caaatgataa ttgttcctat tttttaaaaa
     6421 acttataaat tattgatata tatgtaaatt aatttttaag atttaaaacc tttgtctcaa
     6481 gaaacacaaa aatgccgtct gaaacgtgtt cagacggcat tggctgcaca gccagtccgt
     6541 ttaaaatttc acgcccttat gtaacgcaac gatccccgca ctcatattat gatagtccac
     6601 actgtcaaat ccggcatcca gcatcatttg ctttaaagtt tcctgatcgg ggtgcatgcg
     6661 gatggattcg gcgagatatt gataactctc cgcatctttg gcaatcagtt tgcccatgac
     6721 cggcagcagc ttgaacgaat aaaaatcata cgcgccttcg aacggcttgt agattttgga
     6781 aaactccaac accagcaatg tcccgccagg tttcaaaaca cgatacatct ctttcagcgc
     6841 ggcatcttta tgcgtcatgt tccgcaagcc gaacgccacg gaaaccaagt tgaaataatt
     6901 gtcagggaaa ggcagttttt ccgcatcggc aagcgacacg ggcaaaatca tgccttcgtt
     6961 caacagacgg tcgcgcccga cggtcagcat agaggaatta atatcggtca gccaaacctc
     7021 gccttccttg ccgacccgtt tcgcccaacc gcgcgacaaa tcgcccgtac cgccggcaat
     7081 gtccaacact ttatcgcctt ttttcaggtg cgccgtgttg atggtgaaat gcttccacac
     7141 cctgtgcagc cctgccgaca tcacatcgtt cataatgtcg tagtttttgg cgacggagtg
     7201 gaacacttcc gccacttttc cggctttctc atcttcgttg accgtactga atccgaaatg
     7261 cgttttctgt ccgcccatat tctaaccacc tccctgatgc cgccctacgg catcccaaac
     7321 ttaacaatct gattgctttt caaaacggga agcacccgcc gccgccaatt tgtccaaata
     7381 ttcctgccac atcgcatcgt atccgtatgc cagtctgtgc agatacgccc aatcgtaaag
     7441 accgctgtcg tgcccgtctg aaaaactgat tttcagcgcg tactgtccga caggctgcaa
     7501 atccgcgatt tggacatccg ccttgccggt ctgcaaaaca tcctgtccca cgccgtgtcc
     7561 gcgcacttcc gcactcggcg aatagacgcg taaaaattcg gcaggcagat ttttaggttc
     7621 gtccccataa gtcaaaacca acacggtacg gtttttcaac aaacggattt caacaggaat
     7681 attattcaaa aaacacctcg gttcggcata caatatccgg caggcgcagg cgtgccttca
     7741 aacggcgcgt attttaacag ctttatcccg ataccgatat tcaaacccac actttaaacg
     7801 gcattgatat ggacaatctc gtatgggacg gcattcccga catccgcaca ctcgaccaag
     7861 ccatccgcaa acacgcaccc ccgctcaacc tgattatctg cctccccgat aatcagattc
     7921 ccgattttca aaccgcacaa gatgcttcgg acgcggaatg ccgtctgaag caccgtttgg
     7981 atcaggcaac gcagtgcctc cagttcgaca gcatcaacct catcgaacac atcctgcacg
     8041 atatccgctt ctggctggtt cccccttcac gcacctgccg cctgcacgaa cacttccacc
     8101 acatttcctg gcagaccgaa gccatctcgc aaaccgaaag caagtccgac aaaccctggt
     8161 ttgcacttcc acaaacatcc gaacggcaaa aaccggaaca catcctcgtc atcggcgcag
     8221 gcatatccgg cgcggcaacc gcccacgcct tagcatcaca cggcatttct gttaccgtat
     8281 tggaagcccg aaaagccgcc caagccgcca gcggcaaccg ccaagggctg ctctacgcca
     8341 aaatctcgcc gcacgacacc gaacaaaccg aactgctgct tgccggctac ggctacacca
     8401 aacgcctgct cagacacatc ctgcccgaat ccgaaacctg gggcggcaac ggcatcatcc
     8461 acctcaatta cagccgcatc gaacaacaac gcaatcacga attgggtttg caaaaacacc
     8521 ataaccacct ctaccgcagc atcacgtctg cagaagccga aaaaatcgcc ggtatcccgc
     8581 tgtccgtccc atacgaccac ccttcatgcg gactctactg gcaacacggc gtatggctca
     8641 atccgcccgc attcgtccgc accctcctcc gccatccgct gatcggactg cacgaagaca
     8701 caccgttaac cgacatttcc tacgacgggg aaaagtggat tgcaagcaca ccaaacggca
     8761 tattttccgc cacacacatc atctactgca ccggcgcgaa cagcccctgc ctgcccgaaa
     8821 ccaacctcgc cgccctaccc ctcaggcaaa tacgcggaca aaccggcctc acaccgtcca
     8881 ccccgttttc cgaacaactg cgttgcgccg tttcaggcga aagctacatc agcccgtcgt
     8941 ggcacggact gcactgctac ggtgcaagtt ttattcccaa cagcagccat accggatgga
     9001 acgacgccga agaaacctca aaccgccaag cattggcaca ccttaacccc gcccttgccg
     9061 aatcattgtt tgctgccaac ccaatccccc aaaaatacca agggcacgcc gccatacgct
     9121 gcgacagccc cgaccacctt cccctagtcg gcgcactcgg cgacattgcc gccatgcggc
     9181 aaacttacgc caaacttgca ctggacaaaa actaccgcat cgatgcccca tgcccatatc
     9241 tgcctaatgc ctacgccaac accgcccacg gcacacgcgg gcttgccacc gcccccatct
     9301 gcgccgccgc cgttgcagcc gaaatcctag gcttgcccca tcccttttca caacgcctgc
     9361 gccacgccct acaccccaac cgcaccatca tccgcgccat cgtcagaagg aaggatctaa
     9421 ccccttaagc ctttgccgca tgccgtcccg accacagaaa atgatattca aataaacata
     9481 ccaatcctga cggtaaaata tcgaaaacac ccataacgtt ttcttcccaa aataaggagt
     9541 aaggaatgcc taacaaattg cctttgctgt ccaagatgaa tgttccgtaa cattcgaaga
     9601 cttccttaac cctgccagcc gtttcggttt ctgtaccccc tgttagataa ggaaggaatg
     9661 aaaaagccct gttgattgta tgaagtaaag ggcgttaatt tttgttaatc ttcccttctc
     9721 aggaactcaa tatataagga tgttaatttt tatttccctt ctcgaggact caatatagtg
     9781 gattaacaaa aaccagtacg gc
//
LOCUS       DAXHRI010000063         9797 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXHRI010000063 DAXHRI010000000
VERSION     DAXHRI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.063"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(12..1307)
                     /locus_tag="ACFM75_001155"
     CDS             complement(12..1307)
                     /locus_tag="ACFM75_001155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6255711.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVAGAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNVVYAVNTYAGLKPIFKPLERKAIAVSTFIIATEPLGERGQ
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPERMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1433..2320)
                     /locus_tag="ACFM75_001156"
     CDS             complement(1433..2320)
                     /locus_tag="ACFM75_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6255712.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2320..3285)
                     /locus_tag="ACFM75_001157"
     CDS             complement(2320..3285)
                     /locus_tag="ACFM75_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6255713.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3301..4425)
                     /gene="potA"
                     /locus_tag="ACFM75_001158"
     CDS             complement(3301..4425)
                     /gene="potA"
                     /locus_tag="ACFM75_001158"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC6255714.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVCNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4620..4727)
                     /locus_tag="ACFM75_001159"
                     /pseudo
     CDS             complement(4620..4727)
                     /locus_tag="ACFM75_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="ACFM75_001160"
     CDS             complement(4806..5075)
                     /gene="rpsO"
                     /locus_tag="ACFM75_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC6255715.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5298..6233)
                     /gene="secF"
                     /locus_tag="ACFM75_001161"
     CDS             complement(5298..6233)
                     /gene="secF"
                     /locus_tag="ACFM75_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC6255716.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTKGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6237..8090)
                     /gene="secD"
                     /locus_tag="ACFM75_001162"
     CDS             complement(6237..8090)
                     /gene="secD"
                     /locus_tag="ACFM75_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC6255717.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="ACFM75_001163"
     CDS             complement(8302..8568)
                     /gene="yajC"
                     /locus_tag="ACFM75_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC6255718.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8696..>9797
                     /locus_tag="ACFM75_001164"
     CDS             8696..>9797
                     /locus_tag="ACFM75_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC6255719.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2506 a         2590 c         2436 g         2265 t
ORIGIN      
        1 gcctgcgacg cttaacggct gtctttccac tgataatatt tcgagccgag gaaagaaccg
       61 agtttgcgca aaaacggttg caggataata ttcggctggc gcaaccgctc aaacggcttc
      121 agacggcatt cgtcccctaa aattgcttcg gcaaccgcca aacctgcaat acccgttatt
      181 gccataccat ggccggaata accttgcgta taaaaaacat tcggggctaa acgtccgaaa
      241 tgcgggacaa ggttggcggt aatgtcgcac tccccgcccc acgaatattc gattttgaca
      301 tcggcaagct gcggaaaaac tttaagcata tcttggcgga caagctcggt catacgctca
      361 ggattgtcga taaactcgtt atccttaccg ccgaaaagca gtctgccgtc cgcgctgagg
      421 cggtaataat ccaaaatatg gcggttgtcg cacacagcca tattgttgcg gataagcccc
      481 tgcccacgct cacccaaagg ttcggtggca ataataaagg tgctgacggc aatcgccttg
      541 cgctccaaag gcttgaatat cggcttcaaa ccggcatagg tattgacggc atagaccaca
      601 tttttgcact cgacgctgcc ttcgggcgtg taaaccagcc aaccgttttg atgcggttcg
      661 atgcgcgtca tcggggattg ctcgaaaatc tgcgcaccag cttcggcagc ggcaccggca
      721 acacccaacg tgtaattgag cggatgcagg tgtccggata aggggtcgaa ttgcgcccct
      781 tggtacatat cgctgtcaag ctgctgtttc agttcagtgt tatcccaaag ttgataatga
      841 gttgcaccgt aatatttttg ggcgtgctca tgccattgtt gtaattcttc ccaatgctgc
      901 gaacggatgg caaccgtggc ataaccgcgc tgccaatcgc aatcaatggc atgtttgcgg
      961 acgcgttcgt ccaccagttc gaccgcctgc aaagactgtt gccaaaacca ttgcgcctgc
     1021 tccaaaccga cctgtttttc aatttcctcc ataccgcagg cgtagtcgct gataacctga
     1081 ccgccgctcc tgccggacgc gccgaagccg atacgcgcgg cttccaacac aaccgtttca
     1141 tgtccctgct ccgccaaggg caatgcagtg cacaaaccac tcaatccgcc gccgatgata
     1201 caggtatcgg ttttcagacg gcattgaagt ttcggataaa cagtatgagg attaaccgaa
     1261 ctgaaataat aagaaggcag atattcttga aaatcagggc gaatcattgt gtttgcttta
     1321 tcaggtgtat tttcggacgg aatgatacag gctgtcgggc catatcgtcc aaacagaaaa
     1381 tcggttgaag aaaacaggct gacccagtca tgcggtcagc ctgccttatt aattaatttg
     1441 ctttctcggc agccaatttt tcttggcggt aggcttctgc cgcttctcgg tcacgcttgg
     1501 ttgcctgcct catcatccaa taattgacga tgatgaccaa tgttccgatg atgccgatta
     1561 ggatggtcgc caagacattc atctgcggat cgagacccaa cttgattttg gagaaaatca
     1621 cctgcggcaa tgtggatgaa ccggggccgg agaggaatga ggtaatcacc aaatcatcca
     1681 aagacagggt aatgccgagc agaaagcctg aagcgatggc aggggcaatc aaaggcaaag
     1741 tgatgacaaa aaagattttc agcggacgcg cgcccaaatc cattgcggct tcttcgagcg
     1801 actggtcaag ctcaaccaga cgcgaacgga taacaacggt aatgtacgcc atacacagcg
     1861 tcgtatgtcc gaggaagatg gtgaaaaagc cacgatcgaa gtagagatgt tgtaaccatt
     1921 cgctgccctg caaaaatatc tgtacctgaa taatcagcag cagcatagac agaccggtaa
     1981 tcacgtcggg catcaccata ggtgcggaaa tcataccggc gaacaaggta ctgccgcgaa
     2041 aacgtttaat ccgcgccatt gcatagcctg ccagcgtgcc caaaacgacg gcagcaagcg
     2101 aagacacaac ggcaatccgc agcgacagcc aagcggcttc caagatggtg tcgttttcca
     2161 gcaatgcgcc gtaccacttg gtcgaaaagc cgccccaaac ggttaccagc ttggattcgt
     2221 taaacgaata gatgaccaaa acaaccagcg ggatatacag aaacgccagc gacagtgcca
     2281 acatcagttt caagaaccaa gataatttgg atttctgcat tatttggctc cttcttccaa
     2341 ttcgcggttt tcataatgct gaaacagggc aatcggcacg accagcagcg cgaccatcac
     2401 gacggcgacg gcggaagcca gcggccagtt gttttgatcg aagaacgcct gccacaagac
     2461 tttaccaatc atcaggtttt ccgaaccgcc gaccagctcg ggaatgacga actcgccgac
     2521 agcagggacg aaaaccagca tggagcctgc aataatgccg gttttcgaca aaggcagggt
     2581 aatcgtcaag aacgatttga ccggtcccgc gcccaaatcg gaagccgctt caagcaggcg
     2641 gttgtcgagt ttcaccagtt gcgtgtatag cggcagaatc ataaacggca gataggcgta
     2701 aaccatcacc aaattgagcg aaaaggcatt gtagaacaaa tccaaaggct cgctgataat
     2761 acccatttta atcaacaggt tgtttacaat accgttatgc ccgagcagac ccatccacgc
     2821 atagacgcgc aacaggaacg atgtccaaaa gggcagcata atggcaagca gcaaaccatt
     2881 gcggacagaa ggattggcac gagaaatcgc ataggcggtc ggataaccga ccaacaggca
     2941 aatcactgtc gtcatcaatg cggttttgat tgaagaccag taggtcatca gatagatatt
     3001 gctgttttca ctgtcgccga acgggttaag ggtatgccaa aaattttgga aaatatcggc
     3061 atagttttga taactgatgg cgatattcag acgacctaaa tcttcgtcga tgctggtcag
     3121 cggcgtaaac ggcggaatgg cgatttcttg ttcggcaaag ctgattttca gcacgatggc
     3181 gaacggaatc agaaacagca ccaaaagcca aatatacggt acggcaatca ccgcacgctg
     3241 ccccggacgg cggaacagtt tgtttttcag tttattaagg ttcattgcat tccccttaaa
     3301 tcaacggaac aacggagtcg gttggttttc cggccagctg atatagacgg tttcgtccca
     3361 agttggcggt gtaatgttgc acacatacca gtaaggggcg gggacttggc tcttgacgac
     3421 gcgcccgttg gcgagtttga tatggtaaat ggcgaagctg cccaaatagg cgatttcttt
     3481 taccgtgcct ttcgcccagt tgtagtcgcc caaatattcg ggtttttctt tatataaatc
     3541 aatatcctct ggtcgaatac taacccaaag gtcctgctcg ctcggaccac ccaaaccgtg
     3601 atcgatgcgg acgtggtttt ccaaaccttc gcattcgata acggcatagt cggcatgatc
     3661 ttcaatcacc acaccgtcaa agatgttggt ttcgccgata aactcggcag tgaagcggct
     3721 gttgggatag tcgtacacgt cgctgggtgt gccgacttgc tgcaactgac cgtcagacat
     3781 aatggcgatg cgggtcgcca tcgtcatcgc ctcttcttgg tcgtgcgtaa ccataataca
     3841 ggttacgccg acttgttcca gcgtattgac caactcaagc tgggtttgtt ggcgcagttt
     3901 tttgtccaat gcaccgaggg gctcatccag cagtagaatt ttcggacgtt ttgccagact
     3961 gcgtgccaaa gcaatgcgct gctgctgacc gccggacaat tggtgcggtt tgcgtttagc
     4021 aaatttggtc atctgaacca ggcggagcat ttcttcgacg cgcgcggcga tttcaccttt
     4081 aggcattttg tcctgtttca gaccgaaggc aatgttttgt tctacggtca tatgcggaaa
     4141 aagcgcgtaa ctttggaaca tcatattgat ggggcgatca tagggtgcaa gtttggtaat
     4201 atcctgacca tcaaggataa tttttccctg attgggactt tccatacccg ccagcatacg
     4261 cagcagtgta gattttccgc tgccggaact gcccaaaagg gcgaagattt cgtgttgata
     4321 aatgtccaag tcgatgttat cgacagcgta attgtcacca aactttttca ccaaaccttg
     4381 gattttgaga taaggtttgg ctgaagacgc agtggttgcg gtcataatgg caatactcca
     4441 ataaaaagac gagtaccggc aaaacggatt ttcgaatggg tgataaaaag ctgtttgatt
     4501 gctggcggga gttaaacgtt tgatgccgtc tgaaactctt gtaaagcgca cgggcagcat
     4561 gaaatggaac aagattccaa agaactttat attatattag tttatgcggt tttcgggcaa
     4621 tatagtggat taaatttaaa ccagtacagc gttgccttgc cttgccgtac tatttgtact
     4681 gtctgcggct tcgtcgcctt gtcctgattt aaatttaatc cactataaat atggatttga
     4741 gcttgtcgga aagcaacaga aaagaaaaca ccgcccattt ttctgggcgg tgtcggaaag
     4801 cgtaattatt tacgcagacc caagcgggta atcaacgcgc gatacgtatc gggctgggta
     4861 cggcgcaagt aggccagcag gcggcggcgt tggctgacca ttttcaacag gccgcgacgg
     4921 ctgtggtggt ctttggggtt ggctttgaag tggggggtca ggtcgttgat gcggaaagtc
     4981 aacagagcga cttgtacttc ggaagagccg gtgtcgcctt ctttgcgttg gaaatcttta
     5041 acgatttgtg ctttttgttc tacggtcagt gccataatga aaactccaaa aatataagaa
     5101 tccccatagg gattcagaca agtttgccaa gcctgaagac aacggcaaac tccctatgcc
     5161 caagatagga caacgtggca ttatgacaca attcccgcat ttctgcacaa tattttaaga
     5221 ccgtggaagt aaccgttaaa aatgccgtct gaagcattgt ctgcttgaga cggcaatgtt
     5281 cagacggcat atgcgcttca aaccacgatt tcttctttct tcttcggttc tttaccgata
     5341 ttgtcgcggc tcaaaccgaa cattagcaga agcgggctgg caaccaatac ggaagaataa
     5401 atgccgaaca cgatgccaat ggtcaacgcc atagaaaagc cgtgcaaggc cgcaccgccg
     5461 aacaccagca tggatacgac catcgcctcg gtcgaaccgt gggtaatgat ggtgcggctc
     5521 atcgttgcgg taatcgcgtt gtcgatgact tccggcacgg catgtccgcg catcgccggc
     5581 ttgcggaagt tttcacggat acggtcgaag acgacgacgg attcgttcac agaatagccc
     5641 aatacggcaa ggatacccgc caagacggtc agcgaaaatt cccattggaa gaaggcaaag
     5701 cagccgagaa taatcacgat gtcgtgcata ttggcgataa tggcagatac ggcaaaacgc
     5761 cattcaaaac gcatcgacag gtaaataatg atgccgataa cgacaaaacc taaagccatc
     5821 aatccattac ttaccaattc ctcaccgact tgcgggccga taaattcgac ttggcgcaag
     5881 gtaacgtcgg gactgtcttt tttcagcaaa tccataacct gattggacaa ctgtgcggaa
     5941 gtaacacctt ctttgttcgg caggcggatc atgatgtgtt tgttcgtacc caatgcctga
     6001 acctgtacat cacctatttt cagcgtatcg aggcgttcgc gcatcttatt gacatccgca
     6061 ccctgctgat attggacttc cattaccgta ccgccggtaa attcgacaga gaaattcaga
     6121 cctttggtca ccaagaagaa cacggcagcg ataaacgtaa ccaacgaaat aaaggtcgtc
     6181 agtttgccgt agctcataaa cggaatatcg cgtttgattt taaagagttc catagcttac
     6241 tccttgcctg ccgcagtttc ggcttcaggt ttccataccg caccgatgga aatattctgc
     6301 aatttgcgtc tgcgtccgta ccacagattg accaacgcac ggaatacgac gacggaggaa
     6361 tacatcgaag tcagaatacc caaacagtgt acgaccgcaa aaccgcgtac cgggccggaa
     6421 ccgaatacca aaagcgcgat accggcaatc agcgaagtca ggttggaatc gacaatggtc
     6481 gcccatgcgt gttggaaacc gagattgatt gcctgctgcg gcggcacgcc ggcacgcaat
     6541 tcttcgcgga tacgttcgtt aatcaagacg ttggagtcga ttgccatacc caaagtcaac
     6601 gccagcgcgg ccatacccgg taacgtcaac gttgcctgca tagcagacaa aataccgatt
     6661 aggaacagta tgttggcact caatgcaatg gtagaaaaga aacccatcag acgatagtaa
     6721 accaccatga atgcagcaac gatggcaaaa ccccataaag tcgaatggaa gcctttttcg
     6781 atgttctcct tacccaaaga cggaccgatg gtacgttctt cgacaatctg catcggcgcg
     6841 gcaagagaac cggcacgcaa cagcaaagac gtatcattgg cttcggctgt cgtcatgctt
     6901 ccggaaattt ccacgcgtcc gcctgtaatg gcagtacgga taaccggcgc ggttacaacc
     6961 tcggattttc cttggtcgat caaaaccatc gccatgcgtt tgccgacatt tgcggcagtc
     7021 agttcgccga aaatgctgcc gcccgcgctg tccaagctca gactgacggc aggtgcgccc
     7081 atttggtcga aactcggttg cgcatcgttg atgttgtcgc ccgtcagctc gacctgtttg
     7141 ctgattagca gagtttcggg acgatctccg ccgcttgaaa gcagctcata accgctcggc
     7201 acgttgcctt ccaatgcctc gcgcaacttg gcaggatcgt cctccaccat acgcaattcc
     7261 aaagtcgcgg tacggccgat gatgtctttt gccttggcag tatcctgaac gcccggaagc
     7321 tgcacgacga tacggtctgc acctgactgc tggatgacgg gttcggccac gcccaactcg
     7381 ttcacacggt tgtgcagggt agtgatgttc tgtttgaccg catcggaacg cactttattg
     7441 accgcctctt ccgaaagcgt caagacgata ttgctgccgt ctgaattcag cgttgcttca
     7501 ggaaacagct tgcgcaactg cggcagtgcc ttttgcacat cacctgcatc ctgcaaaggg
     7561 acggtcaggc tgtttccagc ctgacgcacc gtgccgctgc ggattttttc gcggcgcagt
     7621 tcgcggcgga tgtcgcccga ataacgttca aacgttttct gcatcgccgc tttcatatcg
     7681 acctgcatgg tgaaatgcac gccgccgcgc aggtccaaac ccaaaaacat cggattggct
     7741 ttgattttcg ccatccattc ggggctgtcc gccaacaggt tgagcgcggt aatataccct
     7801 tcgcccaaag tgttttcgat gacgtcgcgc gctttaagct gcgtttctgt gtctttgaaa
     7861 cgcactttca gtgaattgtc cacaacaaac atcccgtcgg tctgaatacc cgcgttttgc
     7921 agcgcggcat ccactttgga ttgagtctgt tcgttgatga tgatggcttg tcggttggtc
     7981 gatacctgca cggcgggtgt ttcgccgaat aggttgggca gcgaatacac tgcggcaacc
     8041 gcaatcgtga acacaatcag cagatatttc cataaaggat aacggttcat cattgttcct
     8101 taatggttgg aaccccaccc tttcggtggt gtcggaatcg ggctatttca gaagaggcaa
     8161 aaacccttcc cagccaggca agaccggaag gcggcatctt gaatatgccg ccctgcgtgt
     8221 cggaacatgg tcaagccttc ggttggaatt caaaacaaag tgccgcattc gggctttcca
     8281 gatgcggctt gtcggcacaa atcaatcgac ttttgcggca atcgcattgc gttccacttc
     8341 gacctcgatt tttgtaccct gtccgatatc cacggtaaaa aactgttcgc cgactctggt
     8401 taccttaccc ttgaaacctg ccgccaagac cactttgtcg ccgactttca aggcggcaag
     8461 cattgcctga tgcgctttga atttcttttg ctgcggacgc atgatcagga agtagaacac
     8521 caccataatc aacactaaag gagcaaattg tgcaacagct tgattcataa tttatccgtt
     8581 ctttctaata tggttgaaaa tcgagagggg tatataataa cataagaccg taaacaatat
     8641 atcgggtttg ccgtccgtac cgaccgtata ccgcagcctg cccgtccaca aacccatgtc
     8701 cttgacccgc ctaatcttga aattctatgc actgttgcgc ctttttttgg gcaaaaacgc
     8761 ccgcaccgca tggatttcgc atcccgcctg tgccgggcac gaacccggcg caaaccatcc
     8821 cgattcgccc gaccgcatcc tctgcatcga gcaggcattg cgccgcgccg gtatttggca
     8881 gcacctccaa accatagagg cggaagaaat cagcgatacg cgcctcgcac ttgtccactc
     8941 gagcaaatat ctgaaccgtt tggaatcttg cctgccccaa aaaggcaaga ttttccgcct
     9001 ggataacgac actgcaatca gcacaggctc gctgtctgcc gcacactttg ccgccggttc
     9061 ggcagttcag gcagtcgaca tggtcatgaa ccgtaaagca tggcatgcct tttgcgccgc
     9121 ccgcccgccc ggacaccatg cgggcagcgg caaagccggc ggattctgcc tgctgaacaa
     9181 cgttgccgcc ggcgtcatgc atgccattgc cgaataccgc ctgaaacgca ttgccgtcat
     9241 cgatttcgat gtccactacg gcgacggtac ggcagaaata ttcaaagacg atccgcgcat
     9301 cctgtttttc aacctgtttg aaaccgacct tttccccttc cccgaaaaca acgatatgcc
     9361 cgacggcggc aatatggtgc acctgccctt gccgccggga acgggcagcc gcacattccg
     9421 cgaagccgtc cgcaggcagt ggctgccccg acttgccgca ttcaaacccg aactggtgct
     9481 gctgtcggca ggattcgacg cacaccgtct agacgaatcg ggcaggctca acctgcacga
     9541 ggcggatttt gcctggctga cacacaaaat cattcagacg gcatcgggct gccccggcaa
     9601 aatcgtatcc gtactcgaag gcggctacac ccttgaacct ctggcacaat ctgctgccga
     9661 gcatatccgc gtgctggcag ggctgggcaa atccgatgcc gcaaccgcct atcagaaaac
     9721 actggaccca acaaaaaaac ggtttgccaa accgaaaacc ggacaggtgc gccaacctac
     9781 ccaatccgac cgttacg
//
LOCUS       DAXHRI010000064         9759 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXHRI010000064 DAXHRI010000000
VERSION     DAXHRI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.064"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(287..4456)
                     /locus_tag="ACFM75_001165"
     CDS             complement(287..4456)
                     /locus_tag="ACFM75_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC6255720.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEYRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTESGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSVALLGRGGIRLSGKIDTGKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4511..6358)
                     /locus_tag="ACFM75_001166"
     CDS             complement(4511..6358)
                     /locus_tag="ACFM75_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC6255721.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHTYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTLARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6770..7999
                     /gene="sstT"
                     /locus_tag="ACFM75_001167"
     CDS             6770..7999
                     /gene="sstT"
                     /locus_tag="ACFM75_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC6255722.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGIDNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8111..9388
                     /locus_tag="ACFM75_001168"
     CDS             8111..9388
                     /locus_tag="ACFM75_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6255723.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSTSSARVRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIF
                     APEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHTENGRSYPT
                     KGRFAAKVDFGSKSVDGIIDSGDDLHMGKQKFKAAIDGNGFKGTWTENGGGDVSGRFY
                     GPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9463..9678)
                     /locus_tag="ACFM75_001169"
     CDS             complement(9463..9678)
                     /locus_tag="ACFM75_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255724.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9671..>9759)
                     /locus_tag="ACFM75_001170"
     CDS             complement(9671..>9759)
                     /locus_tag="ACFM75_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6255725.1"
                     /translation="VTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT         1955 a         2490 c         3022 g         2292 t
ORIGIN      
        1 acctgtgtat cgctgtaaat cttagagatg gcggaatata gcggattaac aaaaaccggt
       61 acggcgttgc ctcgccttgg ctcaaagaga acgattctct aaggtgctca agcaccgagt
      121 gaatcggttc cgtactattt gtactgtctg cggcttcgtc gccttatcct gatttttgtt
      181 aattcactat atcgattctg ttcccttgca ggcggacggc ggcggcggac ggtggaaaag
      241 caggttttca aatgtccggt ttggcggcgc gccgtctgaa aaccgcttat tttcctttgc
      301 tgtttccggc ggagtctttt ttgtccgaac cggagaagcg gtcgaaacgt atggtgtatg
      361 tcagctcgcc gcccgacgaa cggctgccga tacgggcaac cgcctgtatg gcgcgggtca
      421 gccggtaaat cagtttgacg gactgttccg cgctggagat gctgtattcg tagccgatgt
      481 agagtttgcc ggtcagttgt ttgccgacgg tcagcacctg ttcggcgggg ttgagttcgc
      541 cggtttgcgc gttgcggctg cgcttgctgg taaagcccaa atcatccacc agcccgatgc
      601 ggtcgttgat ttgcccggca agcagcgcgc cggcggctgc ggacagggcg gcattgtcgc
      661 cgctgctgcc gctgccggcg cggttgagga tgagccagga gagcttgtct ttttcactca
      721 tcggttcgtt tgccgtcagc gtaatgcgcg gactgttgag gctgcccaat atttccacgc
      781 ccgcaccgac gggggaaagg cggcgttcgg cgcggatgtt gaggttgggg tcgttgagcg
      841 ggccgacaaa ggagaccgtg cctttggtaa tgtccaaatc ctgcccgtat gccttataac
      901 gccctttgat gacgcggacc gtgcccacgc cccgcacgct tccgcccgat tgggcggtca
      961 gggtcagttt gccgcctatg gtaacgtccg cgccgtagcc ggcgaagcgg atgccgtcat
     1021 tgaggtctaa agtcaggttc atattgacgg ggagcggtgc cgccgcctct tttttgactt
     1081 cgcctaatac gacgacatcg tcgccgacgg acggcatcga ggatttttgc gaaccgaaca
     1141 gcccctgatc cgttttaatc atcccggtaa cggatatgcc tttttgcggc gaatagcgca
     1201 ggcgggtgtt gccggaaacc gtcaggcggc ggttggggcg ggacaggatg cggtatttgt
     1261 cgaacaccgc gccgatatcg acatcgggtc cgctgttttc cataccgacc gtaccggaga
     1321 gttccgccgt cccttcgtgc cggaatttca ggctgtcgat tacccatttc ctgcccgcga
     1381 tatgcgaacg cagcgagccg ttgtccaaga tgatgccttg ggtttggttg cggtaataga
     1441 gcttgtcgcc gttgatgctg ccgcccaagt gcgggtcggc gatgctgccg ccgagggtta
     1501 cggcggcatt caggctgcct ttgacggttt gtccgacggg caggaagttg cggaatactt
     1561 cggcatcggc aacggtcagg ttgagtttcc cgcccagggg cgaggtactg aaagaattac
     1621 tttgcccgac ggtgatgttg ccgttgattt tcccgtagtt gctgctgccg ttgacggcgg
     1681 cattgacgga cggagagccg acccgtccgc cgatttgcgc ggcggcattc aggctgccgg
     1741 taatgttttg cgcggcggcg ggcagaaagg gcttcaatgt gcccaagtcg ggaagggagg
     1801 cggtaatcct gccgccgagc ggtgcatttg ccatattgcc gccgaaggcg ttgccgatgt
     1861 ccaaatcggc gttaatccgc ccgaaacgcg cgccgccgtc aagcaggatt ccgatgcggt
     1921 cgttttgaaa gcgcgttttc agggaaaatg cgttcaaacc caaagcctgc ccgccgggca
     1981 atacggcatc gccgctttgc cggctgatat tgaggtagcc gcgcgcgttg cgtccgtagg
     2041 cgacatccca gtcgccgttt aaaaccagat tgtgttcgaa gggcggtttg aagaaattgt
     2101 gcaactcggc gatatgcaga ccgtgtgcgc cgccttttgc cgatatgccg gtttttttat
     2161 cccaagaaaa gtgttgcagg ttgaggctgc cgcccattgc ctgccaattt gccgcacttg
     2221 ccgccacgcg ttccgcaccg gcttcgagcg tcatacggtt ttgcagcttg aggttgaatg
     2281 cgccgccgat gtcgaggatg ccgatgctgc ctttccatcg ggtaagttcc ctgttgatgc
     2341 cgcctgaagc gtccaaatcg aatttgaacg gtttgccatc cagcgtcatg gcggcgtgtg
     2401 tgcggatgcg gtgctgcacg cccgtgccgt ccagcatcag gtcggcggta tcgacaaccg
     2461 ccgctccgcc cgacagcgaa aggcggctgc ctttgatgtc ggcgcgtatc gggcggcttg
     2521 tgtcgggcga acctttgagc gtgaaatcga gcgaacggat gtctgccgcc ttgccgatgt
     2581 gcaggttgcg cgccgcgccg gaaaggtcgg tttcaaaggt tcggatgcca ccgtccaaat
     2641 cgccggaaag gtgtccgcgt acatttaaag accccgcgag tccgaaaccg aaacgggata
     2701 aatcgggtgc ggtgatattg aggttaagcc ggtcgccttt tttgccgaag ccgccgtctg
     2761 ttttaataat gttccgcccc agccgcaaat cgacggcggc acgcggaagg tggcgggact
     2821 cgtaaacaat gtcggcactg ccggcaatcg gtacgccgtt gaacgtgccg ggtaaaaacc
     2881 gcattttgcc tgtgaatttt tcttttgcca gttcgccggc aaggtttatt gagccgttga
     2941 tattgcctgc cggaagttgt ggatcgatgc gcgaagggtc gaatgcgcgg gaacggatgt
     3001 ccagcttgag caggcggtct ttaaacagct cgagatagcc ttgcgcggtc aggctgcctt
     3061 gcccggcggc gatgttgacg gtgtcgagca ccagtttccg ctgtccgttt gcggggtcgc
     3121 ttgcaatggc gaggctgccg tccgtgcgcg ccgtgccgat gccgagttgc caagagattt
     3181 tgggcgaggc ggtcgtgcca ccgatgccga tgctgccgtc caacctgcct ttgaacgcgg
     3241 tttgcagtac gtcttccgcg ccgacggagt tgatgcctat atttaaatcg aggatgtctt
     3301 ttccggtgtc gattttgccc gacagcctga tgccgccccg tccgagcagg gcgacggacg
     3361 tattgccgat atgcaccgtg ccgtcctgcc ggatgacaaa gctgcctaaa acctgacgga
     3421 cggggatgcc gttgcggtcg gcaaagccgg ctttggtgtt ttccaaatcg agcgagcctt
     3481 ccagcgcgat gccgtctgaa aacgacggga tggcggtcag gtcgaaattc agcccggcat
     3541 cgggcaggga aggcacgaag gcggacggat tgatgttgaa ccctttgacc agtacttctt
     3601 ccaatgtttt atccaatgat tcggcaaacg ggtggatgac ggattttccc gagaggcgga
     3661 tattgccgcc gtcgatcgcc agttcggcgc gcacatcctt caggctgccg ctcagccgag
     3721 ccgtactgtg tatggttttg ccttcgagtc cgcctttggt gtaaatggcg gtatcgaggg
     3781 caaacggttt tttcaagccg accgaggctg accccgacga actgctccac ggcgtgtcgg
     3841 cggccttcag gtcaaggcgg tgtcctttgc ggtcgtaacg gtatgaagca tccagccgtt
     3901 cgagatagac ggtttgtttg tcaaaggctt tgcccatgct gattttgccc gtctcgaagc
     3961 ggtcgagata gacggcggca ggcaggtcta tgctgtcggg aaggctgagc ggcgggcgtt
     4021 cttctttagg cggagtcggt ttggtaacga tggcgatgtc gccggcggaa atttcggtaa
     4081 tgtgcaggct gcggcgcatc agttcggacg gtttccacgc gaagcggaag cggctgattt
     4141 taaggtctgc cccctcggtt tctatcgacc agttgtcgcc gtcgaagccg tcgagcagcg
     4201 tgcctttgag gttttgggag gaaatgttta cgccgaacca agacgggatt tggtacagcc
     4261 cgaagcgcaa accggattcc gtaccggcga gccagccgag gaaacatact gccaaaatca
     4321 ggacagacag cagtgccgcc gacagcttca gcagcgggcg gcgttttttt gccggcgggg
     4381 tggggcggta ttcagacggc attttgcgcg tgccgttttc ggtcggatcg gtatctgtcg
     4441 gtgcggtatc ggtcataatg tctgtttcaa aatggtttgc tgcaatgccg tctgaagcgg
     4501 ccgtatcggt ttagaagcgt gttcccaagc tgatgtgcca gcggattttc ttatcgctgt
     4561 gcccgtaggc gatgtcgaag gaaaacggcg caagcgggct gaaccagcgc acgcccagtc
     4621 ccgaaccgtg tttcagcttc atacgtttga aattggcggc ggcatcgccc atatcgtgga
     4681 ataccgcgcc ggaaagggtg cgcgtaaacg gcagttggta ttccaggctg cccaccaaga
     4741 gggcgcgttc gggcaggacc gatccgttcg ggccggcaag tccgatgctg tcgagttcgt
     4801 aaccgcgcac ggaagacgcg ccaccgctgc ggaacatcag ccccgagggg acatcggcat
     4861 tgtcgcgggc aagggtgtaa cccgcttgtc cgcgtatgat gaacgtgccg agtttcttgt
     4921 tttcgggcgt gaagaaataa cctgcgcggg cagaggtgcg gatcagcgcg gtggaggaca
     4981 ggaatgtgcc caaagtcgta ccgattttgc cgtcgaggta atggccgttt tcgggatgca
     5041 gcacgttgtt gagcagctgg cgtttccaag aggcggtcag catcgtggcg tggctgttgc
     5101 ccaaatcgat atccgagccg gggattttcc ggccttctgc gagaaactct gcccccagcc
     5161 tggcatcgat gcccgcgcgg tcgcgcacat accagatgcc gccggagaag gcgcgttttt
     5221 cgaggttttg ggtggtcgaa cggttgtagg aaacgttgct tgtccagtag ttgccccgat
     5281 agttgcgcgg ctggctgatg ccggcggcaa gcgtggtttc gtatttgtcc atatcccaga
     5341 cgaccgagcc gatatagcct ttgttgaaga ggttgtaata gtcgtaggcg attttgccgc
     5401 ccaaaccgta ttccgaatcg aggcggatgc cggtttcgag cttgtggcgt ttgacctcgg
     5461 ttacgctgac tttgacgggg acgcggtcgc cttggagacg gtcgaagtcg gcttgtacgg
     5521 acgcgccgga ataatgcccg ttttgttcga gtgcctgttg gaagtcgagc agcaggtcga
     5581 ggtcgtaggg cgtgcccggt tggaagcgcg ccaggccgga gacgatttgt tcggggtaac
     5641 gctgtgtgcc ggtgatttca aagtcgccga aggcgatggg gcggccgctg tccacgacga
     5701 cgttcaaatc gacggtggcg gtatcggggt tgacggccgc ccgggtgttg ccgagcttgg
     5761 caagcgggta gcctttgcgc gttaccgcgc cgaggacgga agttttgctg ttttcccaac
     5821 tgtcctgatc gaaatcactg cctaccggct gctgccagtt ttccagcgcg ttgcggtagt
     5881 attcggcgag gttgccgtct gaaaggatgt cgccgaggat ggcgacaccg acgttggcga
     5941 ttttggtgcg cgggcccggt gtgatgtgta ccgtataagc tccgtctttt tccgtcaggc
     6001 tgactttgct gctgaaatag cctttgctgc ggagcattgt tttaacgttg tccggtgctt
     6061 cttcggcgag gaagcccgtc tgttccttgt ccaatacttc ttcctgctgc tgcgtgatga
     6121 gcggcaggtg ttcttcgacc atatctttga tttcactatc ctgcgtgtcg atgcggacgg
     6181 ggaatttggg ttttaattta actgattcgg tgtcggggct tttgtttttg aacaatgcga
     6241 aacccgccgc cttgttttcg gaaaggtcgg cggcaggcgc gtatgtgtgc ggaaagaaaa
     6301 ataaagccgg caggagcagg gcggtcggtt tgatcatcat ggtacgggtg tcgtgcatcg
     6361 gtgtggcaga tttcgattct aacactgttc ggacagggtt tcgaacggct gtttttgtgt
     6421 tgttcggacg gggcgggaca cttcagacgg catactgccg ccggtatttc ggaggttgcg
     6481 gcccgccgga aatgtatctg cttgttttaa ggtattttgt ttccaatccg catatggaaa
     6541 acaggctgcg tttcggaata gtgccggaaa ataggcagga ttaaataaat ttattttcgt
     6601 tcaataaatt aaaccgttgc gccgaatgat aaagcaaagc ctgtccggca ctgcataggg
     6661 caaatcaacg caaccttaaa acacagccga aaaataaaaa aaaacacaga gaatgagttt
     6721 tcttttaaaa tacaacattt tttaacatat cacaacataa ggaagaatta tggcgttcgg
     6781 caaatcctta tttcatgcga tcggccgcgt cagcctggtc aggcagattg ccgccggttt
     6841 ggcgttgggc atcgtaatcg gttcggtttc accgcaactg ggcttggcgg caggcttgtt
     6901 cggcagcctg tttgtcggcg cgctcaaagc ggtcgcaccg gttttggtat ttattttggt
     6961 ggcggccaca atcgcacagc accaaaaagg caacaaggcg catatcaggc cgattatcgt
     7021 cctctacctc atcggtacgt tttccgcagc cctgaccgcc gtcatcgccg gtatggtttt
     7081 cccgacgcac attgttttgg cgggcgcggg cgatgtgtcc gccgcgccgc cttccggcat
     7141 tgtggaagta ttgaaatcgc tgctgatgaa cctggtcgcc aacccgatta acgcgattgc
     7201 taatgccaac tatatcggca ttctggcttg ggctttggtt ttgggcgcgg cgttgcggaa
     7261 tcacggttcg gacgttacgc ggcaggtcgt tgccgatttg gcggaagcgg tttccaccgt
     7321 cgtgaaatgg attatccgtt ttgccccttt gggcattttc gggctggtgt cgtccacaat
     7381 cgcggagacg ggtttcggcg cgctggcggg gtacgcgaag ctgcttgcag tgctgttggg
     7441 ctgtatggcg tttatcgcgc tggcggtcaa tcccgccatc gtgtggtgga aaatccgccg
     7501 caacccttat ccgctggtgt tcacctgcct gcgcgaaagc ggcgtgtatg ccttctttac
     7561 ccgttcttcc gccgccaata tccccgtgaa tatggctttg gcgaaaaaac tggggctgca
     7621 cgaagacact tattcgattt ccatcccgtt gggggcgacc gtcaatatgg gcggcgcggc
     7681 gattacgatt accgttttgg ctatggcggc ggcgcacacc caaggcatac aggttgattt
     7741 tgccaccgcg ctgctgttga gcctggtggc gacggtcagc gcgtgcggcg cgtccggcgt
     7801 ggcgggcggt tcgctgctgc tgattccgct ggcgtgcagc ctgttcggta tcgataacga
     7861 tgtggccatg caggtggttg ccgtcggctt tatcatcggc gtaattcagg attcggcgga
     7921 aacggcgttg aattcttcaa ccgacgtttt gtttaccgcc gccgccgatt tgggccgaca
     7981 gaggaaccgg gcggaataaa ccaagctatg ccgtctgaag cccgtttggc gttcagacgg
     8041 catattttta caattttatg ggcatatggc ggaaacaata ccggctgcct tgaatatgaa
     8101 ggggatgatg atgtttgaac gcagtgtgat tgcaatggct tgtatttttg ccctttcagc
     8161 ctgtgggggc ggcggtggcg gatcgcccga tgttaaatcg gcggacacgc tgtcaaaacc
     8221 ggccgctcct gttgttgctg aaaaagagac agaggtaaaa gaagatgcgc cacaggcagg
     8281 ttctcaagga cagggcgcgc catccacaca aggcagccaa gatatggcgg cagtttcggc
     8341 agaaaataca ggcaatggcg gtgcggcaac aacggacaaa cccaaaaatg aagacgaggg
     8401 accgcaaaat gatatgccgc aaaattccgc cgaatccgca aatcaaacag ggaacaacca
     8461 acccgccgat tcttcagatt ccgcccccgc gtcaaaccct gcacctgcga atggcggtag
     8521 caattttgga agggttgatt tggctaatgg cgttttgatt gatgggccgt cgcaaaatat
     8581 aacgttgacc cactgtaaag gcgattcttg taatggtgat aatttattgg atgaagaagc
     8641 accgtcaaaa tcagaatttg aaaatttaaa tgagtctgaa cgaattgaga aatataagaa
     8701 agatgggaaa agcgataaat ttactaattt ggttgcgaca gcagttcaag ctaatggaac
     8761 taacaaatat gtcatcattt ataaagacaa gtccacttca tctgcgcgag ttaggcgttc
     8821 tgcacggtcg aggaggtcgc ttcctgccga gatgccgctg attcccgtca atcaggcgga
     8881 tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca atatcttcgc
     8941 gcccgaaggg aattaccggt atctgactta cggggcagaa aaattgtccg gcggatcgta
     9001 tgccctcagt gtgcaaggcg aaccggcaaa aggcgaaatg cttgctggca cggccgtgta
     9061 caacggcgaa gtgctgcatt tccatacgga aaacggacgt tcgtacccga ccaaaggcag
     9121 gtttgccgca aaagtcgatt tcggcagcaa atctgtggac ggcattatcg acagcggcga
     9181 tgatttgcat atgggtaagc aaaaattcaa agccgccatc gatggaaacg gctttaaggg
     9241 gacttggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggcc cggccggcga
     9301 ggaagtggcg ggaaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt
     9361 gtttgccggc aaaaaagagc aggattgatg ttgatgccgt ctgaagcggg gcgttcagac
     9421 ggcattgtga tgtttgggtc gcggctccgg ttcaagtccg catcagttga taaacccgtc
     9481 gtctttcatt ttcttttcgg aaaaatactg gccgaatacc agcagggcgg cgaggatgat
     9541 gacaaacagc cccgccatag tcagtgtgat tttgaagaac aattccgctt cgagaaaaga
     9601 aaaccaaagt tgcacaatca gcagcagcac ccagccggca aacagtccga gacaggcata
     9661 aacgccgagc ttaaacacgg tcccgctcca aaatgacgac gtaggcctcg cgtttgaaaa
     9721 acaacagcat acggcgcaaa tctttttcca tcgttacga
//
LOCUS       DAXHRI010000065         9725 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXHRI010000065 DAXHRI010000000
VERSION     DAXHRI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.065"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            47..1120
                     /locus_tag="ACFM75_001171"
     CDS             47..1120
                     /locus_tag="ACFM75_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC6255726.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGNILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(1177..2703)
                     /gene="ilvA"
                     /locus_tag="ACFM75_001172"
     CDS             complement(1177..2703)
                     /gene="ilvA"
                     /locus_tag="ACFM75_001172"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC6255727.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            2851..4020
                     /locus_tag="ACFM75_001173"
     CDS             2851..4020
                     /locus_tag="ACFM75_001173"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC6255728.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVEKRSRWQRLWVRLTGQ"
     gene            complement(4145..4717)
                     /locus_tag="ACFM75_001174"
     CDS             complement(4145..4717)
                     /locus_tag="ACFM75_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC6255729.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(4784..5767)
                     /locus_tag="ACFM75_001175"
     CDS             complement(4784..5767)
                     /locus_tag="ACFM75_001175"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC6255730.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(5934..6009)
                     /locus_tag="ACFM75_001176"
     tRNA            complement(5934..6009)
                     /locus_tag="ACFM75_001176"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5974..5976),aa:Gln,seq:ttg)
     gene            complement(6018..6093)
                     /locus_tag="ACFM75_001177"
     tRNA            complement(6018..6093)
                     /locus_tag="ACFM75_001177"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6058..6060),aa:Gln,seq:ttg)
     gene            complement(6106..6181)
                     /locus_tag="ACFM75_001178"
     tRNA            complement(6106..6181)
                     /locus_tag="ACFM75_001178"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6146..6148),aa:Gln,seq:ttg)
     gene            complement(6189..7034)
                     /gene="ispE"
                     /locus_tag="ACFM75_001179"
     CDS             complement(6189..7034)
                     /gene="ispE"
                     /locus_tag="ACFM75_001179"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC6255731.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            complement(7044..7625)
                     /locus_tag="ACFM75_001180"
     CDS             complement(7044..7625)
                     /locus_tag="ACFM75_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC6255732.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPLVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(7765..9609)
                     /locus_tag="ACFM75_001181"
     CDS             complement(7765..9609)
                     /locus_tag="ACFM75_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6255733.1"
                     /translation="MLPNRFKMLTVLTATLIAGQVSAAGGGAGDMKQPKEVVKVFRKQ
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGREKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEDISVIDGYVEKAY
                     GRGTEEQRSRAALTAAMMYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
BASE COUNT         2425 a         2764 c         2262 g         2274 t
ORIGIN      
        1 ccactataca agacaaaaaa ctcgccgccg ccgaaaggaa cgcagcatga gcatcaccat
       61 tcaaaactta aacaaacact tcggcaattt tcacgcgctg aaaaacatca acctcaacgt
      121 ccccaccggc aaactcgttt ccctgctcgg cccgtccggc tgcggcaaaa ccacactttt
      181 acgcattatc gccggactgg aaaacgccga cggcggcaat atcctgtttg acgggcaaga
      241 cgtaaccgcc aaacatgtgc gcgagcgcaa agtcggcttc gtgttccaac actacgccct
      301 tttccgccat atgaacgtgt ttgacaacgt cgctttcggt ttgaccgtat tgcccaagtc
      361 cgaacgcccg tccaaagaac aaatctgcac taaagtcgaa gaattgctca aactcgtaca
      421 actctcgcat ttggcaaaat cctatccgca ccaactctcc ggcgggcaac gccagcgcat
      481 cgccctcgcc cgtgcgcttg cggtcgaacc gaaactcctg cttttggacg aacccttcgg
      541 cgcgttggac gccaaagtac gcaaagaatt acgcacctgg ctgcgcgaca tccatcacaa
      601 cctgggtgta accagcattc tggttacgca cgaccaagaa gaagccctcg aagtttccga
      661 cgaaatcgtc gtgatgaacc acggcaaaat cgaacaaacc ggcagtgccg aagccattta
      721 ccgcaaaccc gaaaatgcct tcgttaccga gttcttaggc gaaaccgacg cttttgaagg
      781 acgcatcgaa aaaggcttct ggcattataa cggcttcgcg tggaaattgg acgcacaata
      841 caaatggcag gaacaaaccg ccaccggcta tatccgcccg cacgaatggc agatcgccgc
      901 cgaacacgaa acaccgatga tttgtgccga aatcgaaaaa gtccacgccg tcggggcatt
      961 gacgcatatt ttggtgaaac acggcaaaca ggacgtgcat atcacgctgg ctggcagcga
     1021 tgccgcgcgt tacccaatcg ccgaaggcaa agaattgaag ctgattccaa aacaggttta
     1081 tgtcttctct caaaacgaac tgattgaata ttcgatttaa ccatgaaagc gcaatgccgt
     1141 ctgaaaggct ttcagacggc attgtgcttt caagcgtcag gcaagaaaca gcttgtacgc
     1201 ggcattttgc gtttcctcgt gatagctgta tcccagactt tccaagaaac cgtcaaatgc
     1261 ggcggcatcg tgcggcggca catcgatacc gaccaaaatc cgcccgtaat ccgcaccgtg
     1321 gttgcggtaa tggaagagcg taatgttcca accgccctgc atatggttca aaaaacgcgc
     1381 caacgccccc ggacgctccg gaaactcaaa actgaccaga cgctcgtttt ctactttgtc
     1441 cgtccgccct ccgaccatat agcggatatg gattttggca atctcatcat cggtcaggtc
     1501 gacattgggc aatcccgcct catccaaccg gctgccgata accgccaaat cctgcgggcc
     1561 tgccgcttga agtccgacaa agatatgcgc tttttcatcg tccccgtagc ggtagttgaa
     1621 ctcggtaata ttcctatttc ccaatatatt gacaaactta aggaagctgc cgcgttcttc
     1681 agggatggta acggcaaaaa taccttcgtt gccctcgccc aattcgctcc gttccgaaac
     1741 gtggcgcaaa cggtgaaaat tcatattcgc accgctggta acggcaatca gggtttggtt
     1801 ttccgcgcct tctcgggcga tataggcttt cagacccgcc aacgccaacg cgcccgccgg
     1861 ctcggtaatg ctgcgcgtgt catcgaaaat atccttgacc gcgccgcaaa ccgcatcggt
     1921 atcgactgta atgatttcat ccaaaagttc tttgcagagg cggaaggttt cgtttccgac
     1981 gactttgacc gccgtgccgt ctgaaaacag cccgacatct ttcaaatgga cgatttcacc
     2041 cgcttcgacc gactgcttca tacagcagga atcgttggtc tgaacgccga taactttgat
     2101 ttcgggacgg acctgcttga taaatgccgc cacgcccgcc gccaaaccgc caccgcctat
     2161 cggtacgaat acggcgcgga ttggatcggg atgctggctg acaatttcca tccccaccgt
     2221 cccctgtccc gcaatcacat caggatcatc aaacggcgcg atataggtta acccttcttt
     2281 ttccgccaac tccatcgcat aatcgtaggc atcgttgtat gaaacgcccc gcaaaaccac
     2341 ctcgccgcca cggctcttaa ccgcatccac tttgattttc ggcgtagtct ccggcataac
     2401 gataacggca cggcagccca aacgctgtgc ggacaatgcc acgccttgag catgattgcc
     2461 cgcgcttgcc gcaatcacgc cgcaagcgag cgcatctttc ggcaacttgg acattttgtt
     2521 gtacgcgccg cgtattttga acgaaaaaac cggctgcaaa tcttcgcgtt tcaaaaggat
     2581 gttgtttttc aaacgtacag aaaggctgcg tgccggttcc aaaggcgttt cgaccgcgac
     2641 atcatagaca gatgccgtca ggatgcggat gaggtaattg gaataaggaa ggggcgtgtt
     2701 cataattcaa tatgggataa tcggtttatt aaaatcgcaa aacccaaaac catacgccca
     2761 agacggcgcg aaagcaagaa aaatccgccc gatcagacac cctaagcgta taatcggcag
     2821 actgaaacac gcacacaatt agaatatttc atgacagcac ataaaatcct gcccgtcctg
     2881 ctttccatca tcttaggcgt ttctcacgca acggccgcat cgcccgcgcc caacaaaccg
     2941 gcggtacacg ccgcccccac gttccaaaca cccgaaaccc tcacagcggc acacatcgtt
     3001 atcgaccttc aaagcagaca gactttatcc gccaaaaaca tcaatacccc tgtcgaaccg
     3061 gcggcactaa cccaactgat gaccgcatat ttggttttca aaaacatgaa atcgggaaat
     3121 atccaatctg aagaaaactt aaaaataccc gaatccgcat gggcttcaga aggaagcaga
     3181 atgtttgtac gtcccggcga tacggtcagc accgacaaac tcttaaaagg catgattgca
     3241 ctatccgcaa acgatgccgc cctaaccctt gccggccggc tgggcaacgg ctcgattgaa
     3301 aattttgtgc aacaaatgaa caaagaagcc cgacgcttgg gcatgaagaa cactgtattc
     3361 aaaaacccga caggcttgag tagagaagga caggtttcca ccgccaaaga cctcgcccag
     3421 ctgtctgaag cattgatgcg cgactttccg gaatattacc cgctgttttc catcaaatct
     3481 ttcaaattca aaaatataga gcaaaacaac cgcaatatcc ttttatatag ggacaacaat
     3541 gtaaacggtc tgaaagccgg acacacagaa agcggcggct acaaccttgc cgtgtcatac
     3601 tccggcaacg gcaggcacat ccttgtcatc acattaggtt cggaatcggc ggaaacacgc
     3661 gcctcagaca acagcaagct gctgaactgg gcattgcaag ccttcgatac gcccaaaata
     3721 tatccgaaag gcaaaaccgt tgcccaaatc caaatttccg gaggcagcaa aaaaaccgtc
     3781 cgcgcaggct tcctcaaaga agcctacatc actctgccac ataaggaagc gaaaatggca
     3841 gaacagattt tggaaaccat acagccgatt cccgccccgg taaaaaaagg gcaaatttta
     3901 ggaaaaatca aaatcagaca aaacggatac accattgccg aaaaagaaat cgtcgcactg
     3961 gaaaatgtag aaaaaagaag ccggtggcaa aggctttggg tgcgtctgac agggcagtaa
     4021 tccgccgttt caaatatccc gcttttccaa caaataaaga aatgccgtct gaaacacggt
     4081 tcagacggca taaaacaaca gggcagtacg tattgcatac gctccgccct gctgctgaaa
     4141 tcaattagcg ttttttaccg gtaacagtgg caacagccag attttcgtta cgtttcaggg
     4201 aaacgctttc tacaccttca ggcagtttga tgtctgacaa gtgcagaatg tcgccggcaa
     4261 ccacttcggc acaatccaaa tccaagaaag cagggatgtt ggcaggcaaa gcaactactt
     4321 caacagaagt gtttaacaga gatacgcggc cgccttgcag tttgaccgct tgggaatttt
     4381 cagcgttaac gatgtgcagg ggaacacgga tacgtacaag ttgatcggct ttcacagctt
     4441 ggaagtcgat gtgttggact tcgcggcgga acgggtgcat ttggaaatcg cggacgataa
     4501 cgtctttggt ttcgccgttc agagacaatt taatcaacgc agtatggaaa gattcttttt
     4561 ccaatgcgta gaatacagtt ttgtgatcca cagcgattgc aacgggctct tggccttcac
     4621 cgtacaaaat gccggggatt tggccttcgc gacgcaggcg gcggctcgca ccagtgcctt
     4681 gtgcttcacg aacagaggct tgaatttcat aagtcatgtt aaatactcca atttaaataa
     4741 aactaccgtc atcggccgcg accagcttaa gacggcttcg ggcttatggc agcaacatac
     4801 tgcctgtcat cacttcttca ttaaagagat atgaaacgga ttcttcattg ctgatgcggc
     4861 ggacggtttc ggccaacaga ccggcaatcg ttacctgacg gatacggtcg cagtttttag
     4921 ccgcttcaga caaaggaatg gtatcggtta cgaccacctg gtcgatttcg gatgaggcga
     4981 tacggctgac cgcctctccg gagaatacgg cgtggctggc ataggctaga acacgttcag
     5041 ccccccgctc tttcagagcg acggcggctt tgcacagcgt atttgcagtg tcaatcatat
     5101 cgtccacaat cagacaggtt ctaccttgaa tatcgccgat gatgttcatg acttccgcca
     5161 cattggcttt cgggcggcgt ttgtcgatga ttgccaagtc ggcattcagg gattttgcca
     5221 cggcgcgggc gcggacgaca ccgccgatgt ccgggctgac gacggtcaga ttttcaatcc
     5281 gctgttgttt gatgtcgttc aacagaatcg gggtggcata aatattgtcc accggaatat
     5341 cgaagaaacc ttgaatctgg tcggcatgca aatcgacagt caaaacacgg tcgatccctg
     5401 ccgaatacag catatttgcc accagtttgg cagaaatcgg aacgcggacg gaacgcggac
     5461 ggcggtcttg gcgcgcatag ccgaaatacg gaatggctgt ggtaatacga cctgccgaag
     5521 cacgcttcag tgcatccgcc atcgtcagga tttccatcag gttgtcattg gtcggcgcac
     5581 aggtcggctg aaggatgaaa acatcgcgcc cgcgtacgtt ttccaacagt tcgacggcaa
     5641 cttcgccgtc tgaaaacttg gatacggaag cattgcccaa agaaatgtcc aaatgcctga
     5701 caacacgttg tgccaattcg ggattggcat tgcctgtaaa taccatcaaa ctgtcgtacg
     5761 cagccatatt ctcacctgat tttatgttta acttccgctc agaaaacaca atgcttccct
     5821 ctgcttgtac caagcccgac attatacctg caacatccga cacaaacaaa ggcatttcaa
     5881 tatttttatt tctatgaaat aaaagcgtgt aagcaggctt acacgctttt atttggctgg
     5941 ggaggaagga ttcgaacctt cgcatgctgg aatcaaaatc cagtgtctta accgcttgac
     6001 gactccccaa aaagggctgg ctggggagga aggattcgaa ccttcgcatg ctggaatcaa
     6061 aatccagtgt cttaaccgct tgacgactcc ccaactcgtt tgacttggct ggggaggaag
     6121 gattcgaacc ttcgcatgct ggaatcaaaa tccagtgtct taaccgcttg acgactcccc
     6181 aacaatgttt atacggacaa ccaaggatgt tttgaaagac cctctgccaa atatgcctcg
     6241 tacaaatctg aaacttgtcg gtatatattg tatgcgctat tcctatcttg acacgccgtg
     6301 aatacacacg caccagaacc tgtcattaag gcaaatccat atcgggacaa ctcggaatag
     6361 gctttccaaa cttcagggta ttctttaaat acaactgcct gcatatcatt tctaaacggt
     6421 tgcagatttt ggaaagtcgg cattatgctt gaggcggaat ttcgtgtcaa gccttcgtgt
     6481 gtgaaaattt ttgcagtgga aacgtggacg ggcggtttga cgatgacata ccactgtttc
     6541 ggaatatcca tttcgtccag cctgtcgcct ataccccgcg caaacgcatt tttgccgaaa
     6601 ataaaaaacg gtacgtccgc ccccagagcc gcgcccgaat caatgagctg ccgctgcgtc
     6661 agaccgcact gccaccaacg gttcaacacc agcaaaacgg ttgccgcatc cgagcttccg
     6721 ccgcccaaac ccgcccctgt cgggattttt ttgtccagcc atatttccac gccggcgggg
     6781 ttgcgcgcat atttttgcag caacgatgcg gcacggtagc ttaaatctac ttcctgcggc
     6841 attccatcaa ccggattgtg caggatgatt ttgccgtcgt cccttagttt caaatatacg
     6901 gtatcctgca aatctatcag gcagaatatg ctttcgatat tgtgataacc gtcttcccgc
     6961 tttccagtaa tcctcaaatc gagattcagt tttgcaggtg cgggaaacgc ctgccgtccg
     7021 tccgcaatat tcatctgtcc gccttatctc gtgcgcgccg cacagcgttc cggggtttcg
     7081 gtttcagacg gcataccgat ttcggtgaaa accagcctga tgttcaaatt tccgttattc
     7141 agttgcaacg ttcggacttg ccccccactg tcggcggttc tgccgacagt ccaaccgtat
     7201 tgttccaata tgccgtccgg caggatgcgg taaggcgcgc ccgccacacg cctgccatct
     7261 gcccagatat gcagatattg gattggcagt ttgaaaccga ccagctgcct gctcaattct
     7321 tccgcacttt ccgcctgata gacatttcct ttgccgtcca ctgccaatgc gccgtccctg
     7381 tcttgacaca actgcccgag cgtactgccc aaaggggtgt tgatattgat ggtttccacg
     7441 agcggttggt atgtccaatc gaaatttgca tacgaacctt tcccttccgc tttcactgcc
     7501 aaccgccctt ctgccgtgaa actgcgggtg tgttcggacg gctgccacag gttttcgtta
     7561 ttttgaggca attgcgcgca agcggtcaaa agcaggatga ccgatgcgga tacggtgtgt
     7621 ttcatcagaa tttccttaac ggatgccatc ctgccggccg gtggattcgg tgtgccgtgc
     7681 aaaacgcgtg cgaactgcgt gccgtttgcc cgaatgccgc agcttcccgg caaaccttat
     7741 gccgtctgaa aggatggacc tgcattattt ccggggtttg cgggaaggtt ggggcaatgc
     7801 gatgccgtga cgtttgagcg tttcccgcca tattttcttg tctcccgtaa ggtgtgccgc
     7861 ctgcgtccat acgtcaaccg cctgatcgcg ttcgcccaat gcccacaaca cttcgcccaa
     7921 atgggcggca acttcgggct cggggtcgtt ttcaaacgaa taccgcagat acggcagcgc
     7981 gctttccgcg tcgcctttca ggtaatacgc ccagcctatg ctgtcgttga cagcggtatc
     8041 gtccgggttg atttggtatg ccgtctgaag cagggcgaag ccttcgtcca aacgtttgga
     8101 atcggtcagc aggctgtagc ccagattatt cataatctga gcgttatcgg gtgcaagcct
     8161 gaacgccctt tcaagatctg aaatcatttt tttccgcttg ccaagccgat cgtaaacaac
     8221 tgaccgctgt accaatgcct ctgcctgtaa ctctgtatta ctgccggcag gcggtttttc
     8281 gataatcttg tccaaccccc tcaaagcctc ccgtttatcg ggcagcttcg acagggcgag
     8341 catctgtatt ttggacaaat tgtctgccgt aaaataccgc ccctgctgtt cgggaagttt
     8401 ccgcaccctg ccgatctgcc gcaaagccgc cctgccgccg tccaactcga cagccgccgc
     8461 agccgccagc acacctttgt cgaacaggta ttccggcgcg gatacttttt tcagccactg
     8521 cctgactttg gcgtaatccc tgcggtcggc atacatcatc gccgccgtta gtgccgccct
     8581 gctccgctgt tcctccgtcc ccctgccgta tgccttttcg acatagccat cgataacgga
     8641 aatatcttct ttccggtttg ccgccaatat cgctgcctga atatacaggt ctgcattcgg
     8701 attgcgttcc aacagcacgt tcaaacgcgc ataggcatca tccagcctgt gcagggaaac
     8761 cagattcata atttccattt cctgccagac ggccgaaagg ttttgggtgt ctgtctgctc
     8821 gaaaaagccg tcgagtattt cgggatattt gcgtgcagtc agacgcaacg tcattaaagt
     8881 ggggggcaat atttccgtat cgagcttcgc caaacgctgc aaagctccga ttgccttttc
     8941 cttttcgcgt ccctgtacgc tgaacaccac atcggcaacc gccgcttcgg gcagatgttc
     9001 atatttcaac gccgcgcggc gaaccgcttt cgatgctttt tgcgccaacc cgtcctgttg
     9061 cacggcggct tgtgccaaca ataaaaacac cctgcggttc tgtccttcgt ccgcctgagc
     9121 cagcacttct tccagtccgt ccagatgctg atttcctctt tccctcagca cgttccgcag
     9181 ccaccccgcc cgtttttgcg ccttacccgg tataggctca atctgccgcc atttctgata
     9241 aatcatttcc gcctgttcaa acgcgttcag cgacacggcc atttccaagg cgcgttcggc
     9301 gacttcgggg gattttgtgc gttccaacat cagcatatag gttgccaaag ccgttcccgc
     9361 ctgccccttt tgcaaggcgg tttcccctcc cagcaacgta aatatctgat taacccgctc
     9421 gcccactgcc gcaagccgtg cgcgttcgtt tttgatttct tcctcgctgt aacgctgctg
     9481 ctttctgaaa acctttacga cttccttcgg ctgtttcata tcccccgcac cgcctccggc
     9541 ggcagatacc tgtccggcaa tcaaggttgc cgtcaacaca gttaacattt tgaaacggtt
     9601 aggtaacata atcatccttt atccgacagc cgtccgaacc ttcagacggc atataatcag
     9661 gcgtaacttt atcacaagca gaatcgtttg tagatttgcg aaaccccgtt caaaaaaata
     9721 aatgc
//
LOCUS       DAXHRI010000066         9641 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXHRI010000066 DAXHRI010000000
VERSION     DAXHRI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.066"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(1..1189)
                     /locus_tag="ACFM75_001182"
                     /pseudo
     CDS             complement(1..1189)
                     /locus_tag="ACFM75_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1335..2039
                     /locus_tag="ACFM75_001183"
     CDS             1335..2039
                     /locus_tag="ACFM75_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC6255734.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKLKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQTDASLEYTDFSVSRERRNEVIDQVSKDAILRFK
                     TRAEKLAGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            complement(2103..2669)
                     /gene="dcd"
                     /locus_tag="ACFM75_001184"
     CDS             complement(2103..2669)
                     /gene="dcd"
                     /locus_tag="ACFM75_001184"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC6255735.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICKTSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2735..3151)
                     /locus_tag="ACFM75_001185"
     CDS             complement(2735..3151)
                     /locus_tag="ACFM75_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC6255736.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGGVGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVSANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIQEVKLPKNK"
     gene            complement(3203..4102)
                     /locus_tag="ACFM75_001186"
     CDS             complement(3203..4102)
                     /locus_tag="ACFM75_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6255737.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4133..5590)
                     /gene="der"
                     /locus_tag="ACFM75_001187"
     CDS             complement(4133..5590)
                     /gene="der"
                     /locus_tag="ACFM75_001187"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC6255738.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5747..6376)
                     /locus_tag="ACFM75_001188"
     CDS             complement(5747..6376)
                     /locus_tag="ACFM75_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC6255739.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQNEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6377..7672)
                     /gene="hisS"
                     /locus_tag="ACFM75_001189"
     CDS             complement(6377..7672)
                     /gene="hisS"
                     /locus_tag="ACFM75_001189"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC6255740.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(7799..8455)
                     /locus_tag="ACFM75_001190"
     CDS             complement(7799..8455)
                     /locus_tag="ACFM75_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6255741.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLEKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVGQIRQARAE
                     "
     gene            complement(8513..8704)
                     /locus_tag="ACFM75_001191"
     CDS             complement(8513..8704)
                     /locus_tag="ACFM75_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255742.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(8793..9272)
                     /locus_tag="ACFM75_001192"
     CDS             complement(8793..9272)
                     /locus_tag="ACFM75_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255743.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISSPFPLNILPAPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFAVLILMPLTLKAV"
     gene            complement(9440..9598)
                     /locus_tag="ACFM75_001193"
     CDS             complement(9440..9598)
                     /locus_tag="ACFM75_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255744.1"
                     /translation="MKTFEKTWSAQYRDMEISVRIFWNLERTGAEVYINGRRVYHNEA
                     EMASASLR"
BASE COUNT         2268 a         2309 c         2462 g         2602 t
ORIGIN      
        1 caccggtatt ttcttaccgt attctgcgat tgcctgtcgg aaaatgcaga tcaacctgcc
       61 tataacggca ttttcgccaa attcgttcag acagttttcc ctaagtcggg caggttcaaa
      121 atcagagtgg tgttcataca ttttgatgag tgcgtcggca agggcatcgt cgtcgtcaac
      181 aggaaccaaa tatccgttgc cgtctgaaac aatagattcc gcaccgccgc agcgtgttgc
      241 aatgacgggc aatccttggg acagtgcttc gatatagacc acgccgaagg tttcggtgcg
      301 gctggcaagg acaaatgcgt cgctgttctt catcaaatcc aagactgctt cgggttgcag
      361 tgcgcctaaa aatgtaacgg catgggtaat gcccaagtct gccgcctgct gtttcagccg
      421 ctgttcttcc tgtccgctgc cgccgatgtt tagacgcagt tgcgggcatt gtgccaacgc
      481 ccgggcaaag gcagtgagta ggacatcgtg tcctttgaga cggcgaaggt gcgagacggt
      541 gcagaacacg aaatgcggtt tgttgttttt ttctctttgt tcaaacggtc ggttgaatat
      601 tccgcccagt atgttggggg aggtactgcc attcgcagcc gtatttgtgt tgcaggacgt
      661 gtgcgaaatg gcggctgaca gcgagcagtg cggaggcgtg cgccgctgct ttttccatcg
      721 gctgccattg gtggggacgg atcagaccgc gcgtgatggt gctgctgtgt tccgtgacga
      781 cataggggat gccgtatttt tgggaaattt tttgggcgag tatgccggca tagttcatgc
      841 ggtgggcgtg aatcaggtcg ggcagcccgt tttcgcggat gtagtgtttg aaagctttca
      901 aacccgcaca cacccagcgg atgcggtcga tgtcgatgaa cggaaagcgg gggaagaaat
      961 acatgccgtg ccatgcatag atgtccaaac cgctttgccg gtattgggca aaaccgtaag
     1021 ggccggtcag gatacttgct gtttctttcc gcagataacg gaacatgggg gcgaggatgg
     1081 cggttttgat gcctttcctc tgcaatgcca gtgcctgatt ttgaaaaaaa atcccgtcca
     1141 catcctgttc ggattgcgga taccatgagg ggatgacgag gacgtgcaag ggtttgggca
     1201 tagtgggatt ccgtatcgga aaggcggtta ttataagaca gacgtagacc gaatatttaa
     1261 attgttgcct tacgctaatg caatttagcg cgccgatatg ctagattggc ggttttatga
     1321 gtgaggatac ggatatgttg cgttctattt tggcggcttc cctattggcg gtatcttttc
     1381 cggcagcggc tgaagcattg aattacaata ttgtcgaatt ttccgaatcg gcgggtgtcg
     1441 aggtggctca ggatacaatg tccgcacgtt tccaagtggc ggcggaagga cgggacaaaa
     1501 atgccgtcaa tgccgagttt gttaaaaaat tcaacagttt taacagaaaa ttgaaaaacg
     1561 gcagctttaa aaccgaattg gtatcgcgca gtgcgatgcc gcgctatcaa tataccaacg
     1621 gcagacgcat tcaaacaggc tgggaggagc gtgcggaatt taaggtcgaa ggtagggatt
     1681 ttgatgcgtt gaaccgtttt attgctgatg ttcagacgga tgcttcgctt gaatataccg
     1741 atttcagcgt gtcgcgtgaa cgccgaaacg aggtcatcga tcaggtcagc aaggatgcta
     1801 ttttgcgttt taagacgcgt gccgaaaaac tggcgggcat tttgggtgcg tccggttata
     1861 aaatcgtcaa attaaatttg ggacacatcg gcagccatat cgcgggaggg ggagctgctc
     1921 aggcaaaaat gcttcgtgcc atgccgatgg cggcaagcgt caatatggag ggtgcggatt
     1981 ctgccgcgca gggtgtggag gaaatcagca tcagcgtcaa tgggacggtt cagttctaac
     2041 cacggataaa caggtagatg ccgtctgaaa ccggatgata cggttcagac ggcatttata
     2101 tttcaggctt taggcagggt aacgccggtt tgccccatat atttgccgtt gcggtctttg
     2161 tatgaggttt tgcagatttc gtcgctctcg aagaagagga cttgcgccac gccttcgccg
     2221 gcgtagattt tggcgggcag gggggtggtg ttggaaaact cgagggtaac gtagccttcc
     2281 cattccggct cgaacggggt aacgttgacg atgatgccgc agcgggcgta ggtggatttg
     2341 cccaagcaga cggtcaggac gttgcgcggg atgcggaaat attcgaccgt gcgcgccagt
     2401 gcgaaggaat tgggcgggat gatgcagcag tcgtcttcaa cggtaacgaa gtttttcggg
     2461 tcgaagtttt tgggatcgac gatggtgctg ttgatgttgg tgaagatttt aaattcgttt
     2521 gcgcagcgga tgtcgtagcc gtagctggac gtaccgtagg agatgatgcg tttgccgtcg
     2581 gcttctttga tttggttcgg ctcgaaaggg tcgatcatgc cgaattcttc gctcattcgg
     2641 cgtatccatt tgtcggactt gatgctcata atgttttcct tgtttcttgc ggtgttcgga
     2701 caaagcattc gggaatgccg tctgaaaacg gggcttattt gtttttgggc agtttcactt
     2761 cttgaatcat gccgttttca catttcataa tgaagagggt ttcgccgttt tgcgagacgg
     2821 taaacctgcc gtgtgccagg ccttttttga acgtgcccga gagtaccatg ttgcggaatt
     2881 tggtactgtc ggaattgaac ggttcgataa atatttcgcg gttggcggaa acggtataaa
     2941 cgccttgtcc gtcgaatttg ccatttttaa acgaaccggt ataaccgcgc ccgtcccggc
     3001 aacgccatat gcctttgcct gaaggaagcc cgtcttttcc gactccgccg tcgtaggtgc
     3061 agccgggttc ttgataggaa gtcagaacgg cggccgaagt ggggagggcg aacatcatgg
     3121 cgggcagtag gaatgcgaga tgtttaagca taagggttat tccattggat tttggctgac
     3181 ggtataaaag ccgtctgaaa aatcaatctt gccagccgcc caaataggaa accagctctt
     3241 ccaacatgat gctgacggat tccgccatca ggatttgcga ggcgaaggcg agaccggcgg
     3301 catcgtcgcc gttgctttcg gcttcttcct gcaatacgtc gaggtattgg atgcgcttga
     3361 gtgtgaagtc ttgcgtgagg ataaaggcaa tttgttcgcg ccacaccaag cccagttggg
     3421 taacggtttt accgtttttg acgtgttgaa ccacttcgtc ggcggttaaa tcttgtttgg
     3481 atactttgac gacgggaaca atatcgcccg tgcctttgag ttcgcaatcg ctgtccaatt
     3541 caaaaccgcc ttcgcaatgc ccttgcaaca gccagccggt catcagggaa gagggcgatt
     3601 gtttggtatt cggcagcgag gcttccaaac cgcccaaagc ttcgcgcagc ttggtcagga
     3661 tgttttctgc tttggcggaa gccgcgttat tgacgagcag gtagccgtgg cgggtgttaa
     3721 acaccgcttc tgtacggctg ctgcgggtaa acgctcgggg cagcaggtcg tctgtaattt
     3781 gctctttaag ctcttgtttt tctttgcgac cgacattgcg ggcttcattg ttttggattt
     3841 ccgctacctt ctcttccaaa atatcgcgga tgacgccggc aggcaggact ttttcctctt
     3901 ttttcagggc gacgcgcaag gtaaagtcgg cagggaaaac gagttccggg gagaatgaaa
     3961 ccggtgcggt aaagccttcg ctgaaccagt ctaagccttg gcaatgggta aattcagctt
     4021 cagcaagttt gtcggcaagt acgtctgcct caggcagctt ttctttgttg agcggataaa
     4081 aactaatctg cttgaaccac ataatgtttc ctattgtttg aaatgtcggg aattatttgc
     4141 tgaattgttt tttcacactg actttggttt tcttcttgaa gcggtttttc tcttcctttc
     4201 ggccttcgcg tttctcaata cggttgctca ggctgacgcg gcgcagcggt tttttcttcg
     4261 gtttgtcttc cgcattttca tacgggtttt ccgaaacatt gtattgaatt ctgagcggcg
     4321 tgccttgcag attgaaggct ttgcggaacg tttgggtcag atagcgcgta tagctgtcgg
     4381 aaatcgcgtg cagcgaattg ccgtgtacca caattacggg agggttcatg ccgccttggt
     4441 gggcataacg cattttcgga cgcaccaagc cggcacgcgg cggttgctga cgctcgatcg
     4501 cgctttgtaa tacgcgcgtg attttcggcg tcggcatctt aatcatcgcc gcgttgtagg
     4561 cagcctgaat gctgtcaaac aaaccgtcta taccgcgctc tttcaatgcg gaaataaagt
     4621 ggaacttggc aaaatcgagg aaatacagtt tgcggttgat atcgcgtttc acttgctcgc
     4681 gacgctcttc gctgatgccg tcccatttgt tgacggcaac caccaaggcg cggcctgctt
     4741 ccaaagcaaa accggcaatc gtcgcatctt ggtcggcgat gtcctgctgc gcgtccaata
     4801 ccaaaacagc gacgtttgcc gcttcaaccg cctgcatcgc tttgataacg gagaattttt
     4861 ctactgcttc atccactttg ccgcgacggc gcacgcctgc ggtatcgatg atggtaaacg
     4921 gtttgccttc gcgttcgaaa tcgatgtgga tgctgtcgcg tgtcgtgcct gccatatcga
     4981 aagcaatcac gcgctcttcg ccgaggatgg cgttaaccag tgtggatttg ccgacgttcg
     5041 gacggccgat gacggcaaag acgggatgtt ttgcatcggc ttcttcggct tccggctctg
     5101 ggaaggtttc caaaatatct tcaatcagat aatacacgcc atcgccgtgc gcacctgaaa
     5161 taacataagg gtcgcccaaa gcaagttcgt agaactcggc ggcaagcacg gccctgttgc
     5221 cgccttcgcc tttgtttacc gccagataaa cagggcgcgg gctttggcgc aggcggtcgg
     5281 caatgatttt gtcttgcggg gtcaggccgg tacggccgtc caccaaaaac acaaccgcat
     5341 cagcttcatc gacagcctgc aaggtttgtt ttgccatttc gtgcaaaatg ccgctgtcca
     5401 caaccggctc gaaacctccg gtatcgatga ccaaataagg tttgctgccg actttgccgt
     5461 gtccgtaatg gcggtcgcgg gtcaggccgg gcaggtcgtg tacgagcgcg tctttggtgc
     5521 gcgtcaaacg gttgaataaa gtagatttgc cgacgttggg gcggccgaca agcgcgatgg
     5581 tgggtttcat gtttgggtct ttctgtgtca agtgcaggct tggaggagtc tgagcacgag
     5641 caggtgttcg ttggaacacg gtaaataggt tcttcgcggc aagaggccat aaagagggag
     5701 ggtaaaatgc cgtctgataa ataatggacg gcatttcaga cggcatttat ttcagcgaat
     5761 caagtttcat ttgaaccaat tcgcgaccga cagaatcttg aggcattttt tctaaagcct
     5821 gtccgtagtt ttttaaggct tcctggcttt ttccctgtgc ggcatagaca tcgcctttgg
     5881 tttccatcag caggggggcg aagtccgctt caaccggcgt gtcgagtgcg gcaagggcgg
     5941 catcgtattt cttctgctgg agcagtacca cgccgaggcg ctgtgccgcc aatgcctgaa
     6001 tcaggctgtc tttttggttg gacaacaccc atttcaaatg gccttcggca acatcgtaac
     6061 gctgcgcgtc aaattcggtt gccgccgcca tcagcgtggc ttgggcggcg gaaatggaat
     6121 gcgggtagct ttgttggagt ttggtcaatt cggcattgat ttcgttttgc ggggctttgt
     6181 tttgcgcctt ttccacgatg tttgccagca ccgccgccgc ttcctgattt tgggaagccg
     6241 cacggttttg gtaaaccgtg tatcccaagt agccgagcgc cgccaaaatc agcacggcaa
     6301 acagccattt gcccgtggtt ttccaaaaat atttaaagtt gtctaactct tgttgttctt
     6361 cgagatgggc tgccatttat gcgttcttcc attgttgtaa agtatgggtt aaatcctcgg
     6421 cggcgacagt ttgctgaccg tgtgcgccgt tcatgtcttt gagcgtaacc gtaccgttcg
     6481 ccagttcgtc ttgcgcgaca atcagggcaa agcgcgcgcc gctgttgtcg gcttttttca
     6541 tttgcgcttt caggctttga tagccggaat gctgcattac attgaaacct tgcgcgcgca
     6601 aggcttgcgc gtatttcatc acctgcaagt ccgccctttc gccttggtgc attgcataga
     6661 catcaggcgc agcgttcact tccagcgagc cgtattcgct caccaaaagc agcagccgtt
     6721 cgatgcccat cgcaaagccg atggacggcg caggtttgcc gccaagttcc tcaatcaaac
     6781 catcgtaacg gccgccgccg cacacagttg cctgcgcgcc gagtttgtcg gtcgtccact
     6841 caaaaaccgt ctgattgtaa taatccaaac cgcgaaccaa gcgcggattt tcaatatatt
     6901 gaatacccaa accatccaac atcgccttga agcgtgcata gtggttttgc gaagcctcgc
     6961 ccaagtaatc caccaaacgc ggcgcggcat tgcagatttc ctgcaaatca gggtttttcg
     7021 tatctaaaac acgcaaagga ttggttttca gacggcgttt gctgtcttcg tccaatttat
     7081 cttcatagcg tgtcaaatat tcaaccaacg ccgcacggtg tgccgctcgc tcctcacggt
     7141 tgcctaagct gttgatttcc aaagtcaggt attcgcggat acccaatttt tcccataagt
     7201 cggcagacat cgcgatgatt tccgcatcaa tatccggacc ttcaaaaccc aaagcttcga
     7261 taccgacctg atggaactga cgataacggc ctttttgcgg acgctcgcgg cggaacatcg
     7321 gccccatata ccacagcttt tgcgggctat tgtacagaag gttgtgttcg accaccgcac
     7381 gcagacaaga agccgtacct tcaggacgca ggctcaaact caaagaatcg tttgaatcgg
     7441 agaaggtgta catttccttg ccgaccacat cggtttcttc gccgatggag cggacaaaca
     7501 aaccggtttg ctcgacaatc ggcgtacgga tttgctgata accgtaagcg cgtgtccagc
     7561 ggccgaccgt atcttcaaac gcctgccaaa acgcagccgt cagtttgaaa tctttttgct
     7621 cgacaggcag aaggtcgttc atgcctttga cggattggat tttttgtgcc atttcaagta
     7681 agaatgctta aatcaaattg agggcgatta tagcggattt ttaagggctt gtgaggatag
     7741 agatggtttg cggacgggta aaaatgccgt ctgaaacagt tttcagacgg catttgactt
     7801 actctgcacg tgcttgcctg atttgtccga ctgcaaactc tgtctgccgc ttgggatgat
     7861 gtgtgaaaaa caatttattt gaaattgtct cggctttttt cggcacgacc gccaaaatct
     7921 tacctgccaa atttccctca cgggtttgcc aagcatccaa aaactgcgcc ctgctcattg
     7981 aaacatgacc cagcgacggg tcggcaagca aaaccgtatt gccgtctatc ccgcgcaata
     8041 ccgagaaatg atcatccttg cggtatttca gatacacgat gacggggatt ttcaactgtg
     8101 cgagctgctc gaaagacagg gcatagcctt tcgcttcaaa acccaaatct ggcataatgc
     8161 gccgcatatc ctcaaacgac gcgcgcatct gctccttatc cagtttttcc aacacttctt
     8221 cttccgtcag cgtttgcccg taaaaattgt tcaaaagcgt cgccaccgaa gccgccccgc
     8281 aggaaaaatc caaatcctgc tttacaatat tgaaatcccg ccgcgctttc caactctgca
     8341 ctttgatttt tccgtaaaca acaggattat cgttaaacat cggtgcagca ttcaaacgat
     8401 aagataaaga aacgacaaca cacgccaaca gaaaaacata tttgaacttc atcatattgt
     8461 ccacataaag ggcagcctga aaatctttca ggctgccctt gtcaaattat tcctagcttt
     8521 cggctttttt ggcaaaccaa acaatccgat tacccgcata atactttcca tttattgaaa
     8581 tccgacaagc cgcgcccaaa aaatgccatg cactgtcgat ttccgcagca atctttgtac
     8641 cgttttcttc aaattccaaa tattcaccca ataataaact tgaaacagaa cgcgtgggca
     8701 gcaagtgccc accctacgct tattcaaata atttgattaa ataaagaata aagaaaatgc
     8761 atcaggaaca aaattataat ctgccacctg actcacaccg cttttaaagt aaggggcatc
     8821 aaaatcaaaa ccgcaaaaaa ataatttttg cattgatttt taatagattt aaaattcgaa
     8881 tatagtgttt ctccagattt aaaaagttta tctttatcta tatatttgat gctttcccta
     8941 tccaaaataa atatttcaaa cattaaaaaa tcattacatg accaagccaa atcataaatt
     9001 tgattcaaat gggtatcata aagataaaaa taataaggtt tgggagcagg taaaatattt
     9061 aatggaaaag gagaactaat tttcttaata tctgatgact gatatccata tctttctata
     9121 ttcttaaaaa tttcatcttt cttaagataa caagacattc tgacaatcac aacctgttca
     9181 tcggatatat tatctatttg catggcgcgt ataacacgcc atgcctgatt aaaattagtc
     9241 tcccttacct tagaatctat tatcttttcc aaagatgaaa atgcccctta tcttgaactc
     9301 tccagttaga acctggaagt ttcgtacctc ttaaatgtgt attaatattt cttatagttt
     9361 cattaaaatg ccacgcgctg ccgatttcaa cggtaatttt cgtaccgctt tcttcaaatt
     9421 ccaggtattc ccccattagc taacgcaaag aagcagacgc catttcggct tcgttatgat
     9481 aaacccgcct tccgttgata tagacttccg cccctgtccg ctccaaattc caaaaaatcc
     9541 gtactgaaat ttccatatcc cgatattgtg cagaccatgt tttttcgaag gttttcataa
     9601 aatttcctat acctgtccaa tcggcacata tcaattgcat t
//
LOCUS       DAXHRI010000067         9633 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXHRI010000067 DAXHRI010000000
VERSION     DAXHRI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.067"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(44..3076)
                     /locus_tag="ACFM75_001194"
     CDS             complement(44..3076)
                     /locus_tag="ACFM75_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6255745.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQVQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETILGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3241..3921
                     /locus_tag="ACFM75_001195"
     CDS             3241..3921
                     /locus_tag="ACFM75_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC6255746.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASASGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3950..4309
                     /locus_tag="ACFM75_001196"
     CDS             3950..4309
                     /locus_tag="ACFM75_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC6255747.1"
                     /translation="MLSNIIPLSIGAALGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            complement(4441..4860)
                     /locus_tag="ACFM75_001197"
     CDS             complement(4441..4860)
                     /locus_tag="ACFM75_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255748.1"
                     /translation="MISIFKAYYDVYEFNGELLKRCIFLDEENNEIIKDRIYEYLIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYNNEFDEEDGW
                     PDLSCSLHTFKTTLIAWNAFLQLPKSIHSVVETVIEE"
     gene            complement(5351..5887)
                     /locus_tag="ACFM75_001198"
     CDS             complement(5351..5887)
                     /locus_tag="ACFM75_001198"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC6255749.1"
                     /translation="MDLREVKLGGETIYEGGFVSISKDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKIVLVRQWRYAANQATLELPAGKLDVVGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5921..6277)
                     /locus_tag="ACFM75_001199"
     CDS             complement(5921..6277)
                     /locus_tag="ACFM75_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC6255750.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGIPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            6333..6935
                     /gene="plsY"
                     /locus_tag="ACFM75_001200"
     CDS             6333..6935
                     /gene="plsY"
                     /locus_tag="ACFM75_001200"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC6255751.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALICALIWLVMAFGFKVSSLAALVATTAAPVAASFF
                     MPHVSWVWATVAIALLVLLRHKSNIGNLLKGKEGKIGEKR"
     gene            complement(6954..7079)
                     /locus_tag="ACFM75_001201"
     CDS             complement(6954..7079)
                     /locus_tag="ACFM75_001201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255752.1"
                     /translation="MFEHIGVVAGVECVSVTEHGMKAVCAEMPSERGNMVSDGMT"
     gene            7023..7868
                     /locus_tag="ACFM75_001202"
     CDS             7023..7868
                     /locus_tag="ACFM75_001202"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC6255753.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     VILIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPSLSREESRKLPEELKKLVPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIC"
     gene            8114..9088
                     /locus_tag="ACFM75_001203"
     CDS             8114..9088
                     /locus_tag="ACFM75_001203"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC6255754.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(9231..9449)
                     /locus_tag="ACFM75_001204"
     CDS             complement(9231..9449)
                     /locus_tag="ACFM75_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC6255755.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            complement(9425..>9633)
                     /locus_tag="ACFM75_001205"
     CDS             complement(9425..>9633)
                     /locus_tag="ACFM75_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255756.1"
                     /translation="GKAENLCVKRDIVNVFINRTAVLKENFAVSDKTLDKNIIFVIIN
                     VKIQFDFLTVNQERKAICSQNTVI"
BASE COUNT         2283 a         2791 c         2180 g         2379 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcggttgt agccgatacg caaggcgcgc tgtacgcccg
      181 aaatgctggc tttgcgcgtt ttcaggacaa cggatacggc ctcgtcgtac atcggatcgg
      241 tttcgtcgtc gccgctgcgc ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa
      301 tatcgtcaac atagtccggt tcgccaaact gcttcagata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaacgcg ccgtgaacgc gctgcggata ggccgtaccc ggcggcagga
      421 acagcatatc gccctgaccg agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc
      481 tgtcgatttt gctggatact tgaaacgcga tacgcgtcgg gatgtttgcc ttaatcagac
      541 ccgtgatgac atcgacgctg gggcgttgcg tggcaagaat caaatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cttcgatttt cttgcctgcc gtcatcatca
      661 ggtcggcaaa ctcatcgacc acgaccacga taaacggcag tttttccaaa ggttcgggat
      721 cgtcgggcgt gaggctgaac ggattgccga ttttttctcc ccttgctgcg gcttcggcga
      781 ttttttgatt gaagcccgca agattacgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg tgccggcaac cagcaaatgc ggtgctttgc
     1081 ccaagtcggt tacaacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggcgaattga agatttcgct caggcgtatc atttggcgtt
     1201 tcgggttcgg aagttccaaa cccatacagg ttttgccgag gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaagt ctttttccag atttagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaatcac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgcctcgg gattgaacag cggcggcaga agcaggtcgg
     1501 ttgtcggcag gtgttcgtat accgcatcgg tttcttcaga ttggaacgcc ccttcatccg
     1561 cttccgtatc cgatacccgg caggacgggc gttcagacgg cacatcttca aacggacaaa
     1621 tcgcctgact gtcatgcccg aacgcttcgg tttccgacag atattgcccg cttgaccgct
     1681 ctgccgcacc ctcactgccg tcatccgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcggcaaga tggtcggtct cggcaatgcg gctgcgttgc acctgctcga
     1801 atcctgacgg cggttcatag gtacggttgt agatttccga tacaggaggc ggcggcggaa
     1861 tatcgattgc gggcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcggcagg ttcttcaata tggatatcgg cagcttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacggtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtccga ctttccggaa tcaggcgcga aatccgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcaggca tcgcggacgg
     2161 ctgtgccgtc tgaaacggac tgtccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctgcatgacg gcggaacggc gcattccgtg cattttcggc tttgactgca gaaaaggcag
     2281 acggatgctg cttctcaaaa tacggctcaa atccgtaatc cgcagactcg gaaaacgctt
     2341 ctttgtccgc atcgaacata cgggaatacg tgcgttgaag cacagggtcg tccaaaccga
     2401 taatctgtag cccttccatc ggggtatcgg acacgcggac tttggggacc gctgtttcgt
     2461 ttttctcaaa tgcatcgata tagcgttttt tcgtttccct taaagccgcg ctgtttaaag
     2521 cacgcgttgc ttcttccaaa gtgatttcct taaaaaccgg acggacgggc gaaatttcgc
     2581 tttcagacgg catcaaccct tcagcaatac tccggtcgaa tgggatgcgg cggttgtcga
     2641 ttacggcagt tgcaatgtct tccgtatcgg cagcctcttc ctccgcagct tctgcttctt
     2701 ccgtttccgc ttcttccgtc ccgtttccac tgtctgaata tccgtcggat gcggtcggaa
     2761 catccgcacg gtttgcagaa gcaggcggaa cgggcctgtc cggttcggat tcgtgcgcca
     2821 gatagtttcg gcagaaacgg acgacaccga caaacagcca atacactgcc gttttgacgg
     2881 catggaaaag catcagggca agttcgggaa aatcgggcat accgtctttg atttcaggca
     2941 gctccgcctg tttttcccct ttcttttcct gccacgcaga aacctcgcgc atccactcgc
     3001 gttcggattg tgcgcggaca aaaaacaagc cggcaagcgc aagcaacaaa ataacgatca
     3061 taactatcca aaacatatgc cgtttcccgc aagacaaacg attaaacggg tttattttaa
     3121 cgtgtttcca tcccaaaaaa aacaaccggt cggcaattct gccgaaaccg attcggcttt
     3181 cggtttgccc ccgccgacag tataatagcc gtcctttccc ttaatctttg cggcattgcc
     3241 atgattttcc aaacagtctg gttttcagat atggtactgt cggtttcgtg gattgtcctt
     3301 atcctgatac tggcagcttc cgcgccgtcc gcattccgct cgcttgcccg ctaccgttct
     3361 gcccttcccc tgtgtaccgt aattttttcc gcagcatggt gtctgaacgc ctctgccagc
     3421 ggcggacagc ttgcccagat gaactaccac ctgctcgccg tcaatctggt tgcactgatg
     3481 gtgggcactt ccgccgcctt ttggcttgcc gcgctgctga tgctgcccta ctgcctgctg
     3541 tttgccggtt ctgccggcgc atatccgccg aacgctttag tgctgattct gcccgcgctt
     3601 gccgtcaacc gcctgtcgcg tatgctggtc aaccgcctgc cgcccaatat tttcatcttt
     3661 attttcgtca acggctttct cgcttccgcc gccggcattc tgttgaccgg gctggtgctg
     3721 accggtattt tggatgctgc aaacgctttt ccgtccgaaa tattgtggac gaccgccctg
     3781 cccgtcttta ttctgctggc gtgggcggaa gcttttctca gcggcatttc aactgccata
     3841 tttgttgcac tgaagcccca ttggatcaac actttcgacg acaaccgcta tctgaagtcc
     3901 gaccgcggca tatggcggta gccttcagac ggcataggca ggcaaaacca tgctttccaa
     3961 catcatcccc ctttccatcg gtgcggcact cggtacaacg gcgcgttggc tgctcaacct
     4021 cgccgttccc gcatcgttgt ctcccgccac gggcaacctg tttgccaact ggacgggcgc
     4081 gctgctcatc ggcatctttg ccgaaaccgt caaccatccg caatggaagc tgctgctgat
     4141 taccggtttt ctcggcagcc tgaccacgct ttccggattt tcactggaaa ccgtaaccct
     4201 gctccaatcg aatcgtcctg cttcagcact tgccaatatt ttcctgcaca cggcaggttc
     4261 gctgctgctg acttggctcg ggctgaagat agggacggca gtcaaataat caaaaaaatt
     4321 gcttcctttg gttttgacac aaactataca agtcctaaca tcttaatcat ggcgcagcat
     4381 ataagcgtgg atttgcagcc caatctatac tttctgattc tattcattaa gccattatgc
     4441 ttattcctca atcacggttt ccaccactga atgaatactt ttaggcaatt gcaaaaaagc
     4501 attccaagca attaaagtag ttttaaaagt atgcagcgaa cagcttaaat caggccagcc
     4561 gtcttcttca tcaaattcgt tgttatagaa aactactttt tcagcagaaa tatcccagca
     4621 aaaaccttgt ccacctcctg aataaacttc attgggatta ttttctgctt gatggataaa
     4681 ttctaaattt tcatctatgt ctttttgtag ataaaggtct cctgttaaaa cataaattaa
     4741 gtattcatat attctatctt taattatttc attattttct tcatctaaaa atatacatct
     4801 ttttaatagt tccccattaa attcataaac atcgtaataa gctttaaaaa tagatatcat
     4861 atcatttcct tatcttccat aaatcggctt cggttgttta aataccgccc gtgccaatta
     4921 ccgttctcaa attcaaattg ggcttttagg tctataacaa agaaaaagcc ttggcaggat
     4981 aaacctacca aggcttttta ttaaaaaaaa cgctctatta ctgtgtctaa aaatgcaaaa
     5041 aaagtaacca tcataaccct tttgccaaaa tcaatatcat catccgatga atgaattgac
     5101 ggtatataag aaagaattaa taagctcata aaccggtaag aaaaatcagt aacaaagata
     5161 ttcattatta actattctta aaaatgatca cgttcgtttg ggtaattatc agcccatacc
     5221 ataccctact cctattacta aagcacgaat aactttaaag gacgcgccca gagacaagcc
     5281 gaaaaggcat atcccatccg ccaaacaaaa tgcccgccga cttactccgg cgggcagctc
     5341 caacaccctg tcaatccttc atcagccagt attgcaaacc gattaatgtc ttgccgtctt
     5401 taatttcatc gtttgccaat gcctgacgga cttcttcttt cgacatcaat acggtttccg
     5461 taatctcgtc ttcgtcattg gcaagcgtac tgcccaagcg tacgccttcc gcctcaaaca
     5521 aatacatttt ttcgttgcaa aaaccgaccg ccgtataaaa actgtacaac aggcgtacac
     5581 tatcggcaac ataaggcgtt tcctccgcca attctcgcag cgcacacgct gccatatcct
     5641 cgcccaccac atccagcttg cccgcaggaa gttccaatgt cgcctgattt gccgcataac
     5701 gccactgccg caccaaaacg atcttttcat catccgttac cgccaagaca catgccgcac
     5761 cgggatgacg gatgacgata cgctgccctt cgttaccgtt gggcagcctg accttatcct
     5821 tgctgatgct gacgaaaccg ccctcgtaaa tggtttcgcc gcctaatttt acttccctca
     5881 aatccacttc atatctcctt tccgtttcct attctaccat tcaatcttca cgcttgccgc
     5941 gctcgatttc cacgccaacc tcgcgcacgc cttccaaaat acccggcttg acgattttca
     6001 cgcgcaccca caccgcgccg aaatatccca aaaccaaatc ggcaatatat tccgccaacg
     6061 cttccaaaag caggaaatcc tgttctttca gatgtcggcg cagcgtttcg catacctcgg
     6121 cataatgcac cgtattggca atatcgtcgt ccgaacccgc tttctcggga atgccgatgt
     6181 ccaaatcgac aatcagggtc tgcaaccgtt cgcgctccca gccgtacacg ccgataagcg
     6241 tatccgcctt gatgccgtgc aaaaagattt tatccataat ccgccccgtt ttttgctaaa
     6301 ataaccgcac cattctaagt aaagtaccgc aaatgttcaa cataccggct gttgccgttt
     6361 cctatctgat cggctcactt tctttcgccg tcatcgtgtc caaatattac ggcatggacg
     6421 acccgcgcac ctacggatcg ggcaatccgg gcgcaaccaa tgttttacgc agcggcaaaa
     6481 aaaaggcggc cgcgctgacg ctcttgggcg atgccgccaa aggtttggtt gccgttttgc
     6541 ttgcacgcgt gcttcaagaa ccgctcggtt tatccgacag cgcaatcgct gccgtcgcac
     6601 tcgccgcgct ggtcgggcat atgtggccgg tatttttcgg atttaaaggc ggcaaaggtg
     6661 tggcaacggc attgggcgtg cttctggcac tctctcccgc aactgccttg atctgcgcgt
     6721 tgatttggct tgttatggca ttcggcttca aggtgtcctc ccttgccgcg ctggtcgcca
     6781 caaccgccgc accggtcgcc gcatccttct ttatgccgca cgtctcgtgg gtttgggcga
     6841 ccgtcgccat tgctttgctg gtgctgttgc ggcacaaaag caatatcggc aacctgctta
     6901 aaggcaaaga aggcaaaatc ggcgaaaaac gctgattcgg catttgtttt ccattacgtc
     6961 atgccgtctg aaaccatatt tccgcgttca gacggcattt ccgcacaaac ggctttcata
     7021 ccatgctcag ttacagacac gcattccacg ccggcaacca cgccgatatg ctcaaacact
     7081 tcaccttgtt tttagtgctg caatatttca accgcaaaga caagccctac tggtacatcg
     7141 acacgcacgg cggcgcgggt ttgtacaatc tcgaaggcag cgaagcacag aaagtcggtg
     7201 aataccgcca aggcatcgcc ctgctccgac aggcgcaaaa cctgcctgcc gaactctctg
     7261 actttgccgc gcacatacaa aaaatcctgc cctcgcccga actttactgc ggttctccgt
     7321 ggctggcgca atcgctgacc cgcgtcggcg acaaattgcg cctgtttgag ctgcacccca
     7381 ccgactttgt ccatctgcaa aacaatatgg gcgaagcagg attgggcaaa cgcggacaag
     7441 tgttgcgcga agacggttac aaagggctga tttccctgct gccgcccccg ccgcgacgcg
     7501 ctgttattct catcgacccg ccctatgagg aaaaacagga ctaccggcgt gtaacggaaa
     7561 cgctgaaagc cgctttaaaa cggtttgagt ccggctgtta tctcatctgg tatcccagcc
     7621 tcagccgcga agaaagccgc aagctgcccg aagaactgaa aaaactcgtt cccgacaact
     7681 accttcacgc cgaactgcac gtccacgcgc cgaaagccga cggtttcggt atgcacggca
     7741 gcggaatgtt cgtcatcaat ccgccctatc tgttggcgga acaattggcg gcaaacctcc
     7801 ccgccctgac gcggctgctg gcgcaagatg aaggcgcgcg ctacctgttg gacagcaaaa
     7861 tctgctgacc ccgatgccgt ctgaaaccct tcagacaaca tctcccgtca aataaacaaa
     7921 accggacgat ataccggcag tcggcacttt taagaataaa tatcccctca tccgcctgcc
     7981 gcgttccgac ttgccccatg ccgttttttt acactacaat cccctagact atcctgcaaa
     8041 acgcaccaaa ggaatacgcc acaataacct gccgcctttc agacggcaca aacctttccg
     8101 aaacgagcaa actatggaca cactgacccg attcctcccc gaacacctgc aacaaaacca
     8161 gctgcccgaa gcactcggcg gcgttttact gtccgttgtt tccgcctgca ccgaaatcaa
     8221 cgccaaagtc cgcctcggcg cactggccgg cgtattgggt atggcgggca cgggcaatat
     8281 tcagggcgaa gaccaaaaaa aactggatgt tatcgccaac aacatcatga ttgacacact
     8341 caaagccaac cctgccgttg ccggtttggc aagcgaggaa gaagactctt tcgtaagtgc
     8401 cggtgaaaac ggacgctatc tcgtcctatt cgacccttta gacggatcgt ccaatattga
     8461 tgtcaacatt tccgtcggta caattttctc catccttgaa aaacccgaag gcgcattggc
     8521 aaccgaatca ttccttcaaa cgggcagaca gcagcttgcc gcaggctata tcctctacgg
     8581 gcctcaaacc cagctcgtat tcacattcgg acacggcgta tatatgttca cgctcaatgc
     8641 cgaaaacgaa tttgtgctga ccaaagaaaa accgaaagta cccgaaagta ccaaagaatt
     8701 tgccatcaat atgtccaacc gccgccactg gctgcccccc gtccaacaat acatcgacga
     8761 gctcttggcg ggcgaaaccg gcacgcgcgg caaaaactac aatatgcgct gggtagccag
     8821 catggttgcc gaaatccacc gcatcctgat gcgcggcggc gtgttcatgt atccgcaaga
     8881 caaacgcgac ccgtccaaac ccggcaaact gcgcctgatg tacgaagcca accctatgag
     8941 cctgatactc gaacaggcgg gcgcatcggc aagcaacgca tatcaagcca tgctcgacat
     9001 acagcccgaa aacctgcacc aacgcgtcgc cgtcttcatg ggcagcagcg aagaagtcgc
     9061 ctatctcgac cgcctgcacg caaaatagac agacagccgt tcacacaagt acacggcggc
     9121 ggcaatccga taccgccgcc gttatttcaa acagcacaag ccttctggtc aaatgacaaa
     9181 aatgccgtct gaaaacattt cagacggcat ttcaaaaact caaacccgaa ttattttccc
     9241 gctgcttttt gcaggatggc gtacaactcg tctttcagtt tcaatttggc ttttttcagc
     9301 tcatcgatgg tttccgcacc gctggtaacc ggattgttga ccagaccggt aattttatcg
     9361 tccagctcgt tgtgttcgtc gaacagacgg gcgaagcggg aattttcctg tttcaatttg
     9421 gaaatcaaat cacggtattc tgggaacata tcgctttcct ctcttggttt acggttaaaa
     9481 agtcaaactg aatctttaca ttaattataa caaagataat atttttatcc agtgttttat
     9541 cggatacggc aaaattttct tttaacaccg ccgtccggtt gataaaaacg ttgacaatat
     9601 cacgctttac gcataaattt tcagcctttc cta
//
LOCUS       DAXHRI010000068         9547 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXHRI010000068 DAXHRI010000000
VERSION     DAXHRI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.068"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     repeat_region   30..263
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=96..131
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
     gene            complement(327..653)
                     /gene="cas2"
                     /locus_tag="ACFM75_001206"
     CDS             complement(327..653)
                     /gene="cas2"
                     /locus_tag="ACFM75_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFC6255757.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLETTEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(646..1560)
                     /gene="cas1"
                     /locus_tag="ACFM75_001207"
     CDS             complement(646..1560)
                     /gene="cas1"
                     /locus_tag="ACFM75_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC6255758.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGCLKTELTLDIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            complement(1626..4873)
                     /gene="cas9"
                     /locus_tag="ACFM75_001208"
                     /gene_synonym="csn1"
                     /pseudo
     CDS             complement(1626..4873)
                     /gene="cas9"
                     /locus_tag="ACFM75_001208"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes.;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
     gene            complement(5152..8586)
                     /gene="dnaE"
                     /locus_tag="ACFM75_001209"
     CDS             complement(5152..8586)
                     /gene="dnaE"
                     /locus_tag="ACFM75_001209"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC6255759.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLVAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSSDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMNNADQKAANTNQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANGKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            8599..8832
                     /locus_tag="ACFM75_001210"
     CDS             8599..8832
                     /locus_tag="ACFM75_001210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255760.1"
                     /translation="MNNKRRIVAGLSLDGGVGNAVSGSDGIACKWFFELLRRQKNASC
                     RMAEASQGVLIDIKDYQTSYKELYTLFGRMASS"
     gene            9120..9353
                     /locus_tag="ACFM75_001211"
     CDS             9120..9353
                     /locus_tag="ACFM75_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6255761.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            9331..9534
                     /locus_tag="ACFM75_001212"
     CDS             9331..9534
                     /locus_tag="ACFM75_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC6255762.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
BASE COUNT         1998 a         2289 c         2617 g         2643 t
ORIGIN      
        1 tccaaagttt taatttatat tattttagtg ttgtagctcc ctttctcatt tcgcagtact
       61 acaatcagaa gatgataact gtgctggggg tgcgtgttgt agctcccttt ctcatttcgc
      121 agtgctacaa tacgcaataa cggcgagttt acgcatcaat ggttgtagct ccctttctca
      181 tttcgcagtg ctacaattaa cgattgaaag tgagtatgta ttaagtagtt gtagctccct
      241 ttctcatttc gcagtgctac aatacttcga cgggaaatcc ttatttcata agggtttccc
      301 gttttatttg ttatgaaaaa atgggcttaa aataacaata attggtctga attgaccttt
      361 ttttcttggg ttttcagctc gcccaacagc agtttcatgg cggcatattg cttttctgtt
      421 gtctccaaac agcggattga gccttcttgc ggaaggtttg cacatagtct attgtggtgt
      481 ttttgcaacg aatcgcggcc tttgacgata cggctgtata cggaaagttg cagcatttgg
      541 tatccgtctt ttaataaaaa ctggcggaat tgattggcgg ctttgcgctt tgccgccgtg
      601 ataaccggta ggtcgaagaa gacgataatc ctcataaatt tggcctcact catattggta
      661 ttctttcaat ggtaggattt caggcagttt cagttgtttg gcgtttttat cggttacgcc
      721 ggcttgaaat gacgaaatca ttttgtcgat ggcagccaag gtgctgaaat gttgccgttc
      781 gatgctggtt tgatagtaga ggattttgat gaggtgttgt ttgatgtcga gcgtcagttc
      841 agttttcaga cagccttgtt cgtacaaatg tatcacggtc aggtcggcca atgggcgtaa
      901 gggttcgata aagtcatcgg cgagattaaa tggattgagt tcacttctat gaaacaagcc
      961 taatgcaggt aaccaaccgt ataaggtcag ggcgcgtgca actgcggcac ggagtacggc
     1021 gtaggtgtag ttcaatgcgg cattgacggc gttgttgtca ttacgggtaa atttttctcc
     1081 aaagagggct tgaaagtaga gggcggcagc ttgggcttca cggtttcctg tatcgcctga
     1141 acggacttca gatgccaaag tgcgcagccg tttggctgca agatcattgc ctgtttcgtc
     1201 tgcgacaaat gcttggttca ggattttttg gcggacgata tgttgccaga gttgcttttt
     1261 taagggttcg cttatgttca gttgcagttt gaggatttta agttggcggt gatattgggc
     1321 ataaggcagc cattgtccgc agggcaggaa ctgctcgtcg caggtcagca gggttgcgcc
     1381 atgttcagct aaggctgata ggagcggggc ggtaatcagg gtttcgcggt tttcgatgat
     1441 gataacggcg atgtcttcta atggtacggt atgggactcg ccgttttgtt gaatcagcag
     1501 ttgccggcgt tgcaaggata gtttgccgcc gttttgaata agtaggctgc gccaagtcat
     1561 gttttcccct tattttgata taaaaaatcc ctgcttcagg ccgtctgaag cagggacaga
     1621 ttaatttaac ggacaggtgg gcgttttttc agacggcatg gtctgatttc tttgcccagt
     1681 tcgtcaattt ggtatttttg gaatgaaagg gcggttttga cgccgatacc ttccagtatt
     1741 ccatttttgc caattttatg atcaagatca tgaatgcgta tattgatatt acctgtgcct
     1801 cgatggcagc tggcaaagta accaaacatt ctagcttttt ttgttataac ctcgactaaa
     1861 tcattagggt gtaatgagaa tttaaagttg aaactatcat caataagttg ccaatcttct
     1921 tcatcttttc cttgtacaac agccctatcc ggcaaaatcc ctttcgctac ctgccaactg
     1981 taaatcggta ccagataata cttgtcgcct ttctcaaaca catctacgcg caccatggtt
     2041 gcgttgtcgg caataccgtt atggttgcgc acccatacgc cggttttctg tacttgctct
     2101 acgcgtacgg cttttacctg ttgggtgcgg ttgcctgctt tatcgtattt gtaaaacggc
     2161 tcggcaaagg ctttggcagg atcgtcttta tgtgcttcca gccgtgcttt cagtgcttcg
     2221 tatagcttag gttcgcgctc ccgattgacc attttttcca agtctttcag ttttaactgt
     2281 gtcagcggta cgcgcaacac gctgacgcct tcgtccagtc gtttggcgga tttgacggtc
     2341 tccatatgcc cttgcccgct catcttccga ttgggcgcgc gtgaaacaaa cagtggcgta
     2401 acgtattcgt gtacggcttc ggggcgagat gataattttt cggcaagcaa cgtgcgcagt
     2461 ttttctaggg tatcggcttc ttcgaattcg ggtttgccgt ccggtttgcc gaagacgcga
     2521 atcatgactt cttgtgcgaa aaattcccaa ggttgtggga agtgtgtttt ttgatgcagc
     2581 acttctcctg tttctttgtc tatggtttta ccgtcaaacg cgttcatctc tttatagcgt
     2641 acaaaacggg taattttctg ctgcatggca acggtcgagc aggcaacgac gacggcgtcc
     2701 aaggcgtgat ggcggtcgtt ttccgcacgc actttgcgca atccccaaaa gccgcgcaac
     2761 agattggtaa tttgtccgtt ggatgcaaag acacgtttct tgcctttacc tgtcagccgc
     2821 atacggtcgg caacaaattg acacaggaaa cggttgacgt agcgcgtgtc gttcagattg
     2881 cgttctttaa agccgtcttc atcgaatttt tgcagcagaa tccgttgttt tttactgcgc
     2941 gggaaacggc tggtttcgac acgcgcttta aattcctgcc attcgcggct gttgtctttg
     3001 ccgttgaagt attcgtaagg ggtttgattg cctttgtttt ggttttcgct gcccaatacc
     3061 agtactttat tgttgaaact gtcgtcccat gtgcgcgaga acggcagggc atggtcgatt
     3121 tcgacatagc ctttttcgtt cagacggcct aagttgattt ctttgcccga atacaggcat
     3181 ttgccgtgtt gttgctcgta caggcgcagt ttcagaatat ctttggattt gggttctccg
     3241 acaaaattgg ggaaatactc tcggaatttg gcggcggctt ttcccggtct ttgcggtttt
     3301 cttcttggcg tttctcaatt tctttgcggt ctttaaacga tttacctact tcccttgcag
     3361 tttcaatatg gatacgagct ggggagccgt aacggcgtac cacgccgtta atgaccttac
     3421 gtgcttgaga taaggcgcgc aagacgacgg ggttgcggat ttcgtcggcg ggaatcggcg
     3481 gcagataaat cttttcttcc gtattcttct tgccgtaatg gtctccgtag atttcggcgc
     3541 aggcttcatc gtaacgtttg ccttgttcca ttagaggcac aattcggcgc aatgctttca
     3601 aggaaatttg gacgaactta tcgaagctga tgtgtttcaa cagcgcttct aagatttcgg
     3661 gctgtatacg gtctttcaga cggcctgtaa tgtcttcatc ggttttgaac agggagaatg
     3721 ccgtgccgat ttcgtcttgt aattcgggag aaaggtttaa tggggatttt ttgtctttca
     3781 atccttcttt ttccagtgca cggctgatgg tatggtaggc cttcatttcc atcaatgttg
     3841 aggcttcggc attgtcttta ccatagcgca agcctttgaa aaaggcggta tcttctaaac
     3901 tcagcagctt acgggcttgt gcgtaagtca gtttggattt tctgtatggc tcgtccataa
     3961 gcgtggcgcg ttcggtatcg gtcaatggcc gctcgctgcc ttgctctaaa atacgcaggt
     4021 tgttcagctt ggtcagccag atgaaacgtt cggctgtgta ggtgttttta gcggctttcg
     4081 gctctgccgg ttcgaaggtg caatgcccca acattttttg aacggcatcg ccggacaggg
     4141 cagggcgttg cgtcatcagt agggtttcaa taccttcttt aaggccgcct gaaacatgcg
     4201 gattgccgaa ttctttttgt ttttcaaaca gtaaattcag ctccgcctgc aaatctttgc
     4261 ggctgaacgt atgcgaataa tcgccgcgtt ggttgcggat atggccgcat tctttctcaa
     4321 atttatttaa agccaattcg gccggtgtgc ggaaatcgcc tgtctgtaag gcatgggcat
     4381 tgtcggctac gcctttgagc aaagcgccaa gctctttatc ggcagtttcg ccctcgtttt
     4441 ttctttgcga caaataaccg cggtgtttga ttaaatgcaa caagactgcc gaccactcta
     4501 aaggcgtcag tttgcggtct aatgcggctg cgcgaagttg ccatggtgta ttcggtaagg
     4561 atttgatcaa gccgttttcg tcaaaatcag cggcttgtaa tacgccttcg cgtttcaata
     4621 ggcggcgggc ccgaagcagg cggtgggcgc gacggcgggt caggcggcgg acactgcgcg
     4681 ccaaacgcct tgccatggca agggagtcgc ctgttttcgg tacttcggca cgctcaaata
     4741 cgcgcacgcc caaatcaatc aggcggatgg ggttttcttc ttcgtcaatt tctaccatcg
     4801 cccagccgac ggatgcgatg ccgatatcga ggccgaggat gtagttgatt gaattaggtt
     4861 tgaaggcagc catttattac tccgtaaaca acgttgcaaa taatgataat atatctgatt
     4921 gtcttattta tatacaaata gattattgac ttatcatctc acaacggcta gaatcccaaa
     4981 catattgtcg cactgcgaaa tgagaaccgt tgctacaata aggccgtctg aaaagatgtg
     5041 ccgcaacgct ctgcccctta aagcttctgc tttaaggggc atcgtttatt tcagttaaaa
     5101 aatgccgtct gaaaccggtt tttgggcttc agacggcatt tatattttgg gttaccaatt
     5161 cacgcgcacc gaccggctgc cgagcaatgt ttccagttcg ccgaacaatg cggaactcgg
     5221 cgtaaccgtc catttcggcg gcacttgaag cctgcccgac gcttttccgt tggcatacga
     5281 cagttgcagc gggatgcgcg gcgtgtcggg cagttggtgg gcggcgagca gttgtaccag
     5341 tccgccgatg tcgtgatgcg gggcgagggc gaggctgagg ctgcgggcgt agcgttcgcg
     5401 cgccgtttgc agggtcatga cttggtttgc cagaatacgc agtccgtcgc cgccgccgta
     5461 gtcgtcgcgg ctgactttgg attcgataat cagcacttgg tcggctttga ggcagtcggc
     5521 gcagttttcc aacgtctgac cgccgaccat gatttcaacc tgtccgctca aatcttcgag
     5581 gctgacgaag gcgattttgc cgcgtttgcc catcatggtg cgtacggcgg taacgaatcc
     5641 ggcgaggcgc acgctgtctt gcggcttcag acggcctaat ttggtcggtg cgatttggcg
     5701 gacttcttgg gcatacgggc cgaacgggtg gccggagagg taaaagccga tgacggtttt
     5761 ttcttcggcg agtttttccg attcgctcca catcggcgcg tcgatgagcc gcaccggttc
     5821 gatggcgtct tccatcatgt cgaaaagtcc gccctgattg gtgttggcgg ctttttggtc
     5881 ggcgttgttc atggcgaggt cgatgtttgc caagagcatg gcgcggttgg gttcgatgct
     5941 gtcgaacgcg ccgccgcgta tcaaggcttc gagggtgcgg cggttcatgt gttctttgcc
     6001 gacgcgctcg cagaagtcca agaggccggt aaacttgccg ccgctttgcc gcgcggcggt
     6061 gatggattcg acggcggctt cgcccgtgcc tttaatcgcg ccgagcgcgt agcggatttt
     6121 catgtccgga tacggcgtga agcggtagtc ggattcgttg atgtcgggcg gcaggaactc
     6181 aatgccgttg gcgcggcagt cgtcgtagaa atgcttgagc tggtcggtgt tgtccaattc
     6241 ggacgacatg gtcgccgcca taaattcggc gggatagtgg gctttaagcc atgcggtctg
     6301 gtaggaaatc agggcgtagg cggcggcgtg ggatttgttg aaaccgtagc cggcgaattt
     6361 ttccatgtag ttgaagattt cgtcggattt ttcgcgcgaa atgccttgtt ttgccgcgcc
     6421 ttcggcaaag atttcgcggt gtttcaccat ttcttcgggc tttttcttac ccatcgcgcg
     6481 gcgcagcagg tccgcgccgc cgagcgagta gccgccgata atttgcgccg cctgcatcac
     6541 ttgttcctga tacaccataa tcccgtaggt cggcgcgagg atgccttcga gcagcggatg
     6601 gatgtattgg aattcctggc ctttcatgcg cgcgacgaag tcggggatgt tgtccatcgg
     6661 gccggggcgg tagagcgata cgaaggcaat cagttcttca aacttggtcg tgtgcgccgt
     6721 tttcagcatt tttttcatgc cggtcgactc gaactggaac acggcggtgg tgttcgcatc
     6781 tcggaagatt tggtaggcgg cttggtcgtc gagcggaatt ttgccgacat cgatgatgtc
     6841 gccggtggtg tttttgatgt tgttctgcgc catttcgata atggtcaggt tacgcagacc
     6901 taaaaagtcg aatttcacca aacccacgtc ttccacgtcg cccttgtcgt acatggatac
     6961 gggagaggcg gattcatccg cctgatacac ggggctgtaa tcggaaatct tgcccggcgc
     7021 aatcaacacg ccgcctgcgt gcatacccaa accgcgcgtc aggtcttcca gctttttcgc
     7081 cagcgtaatc agttcgtccg cttcttccgc ttcgattaat tcctgaatct gcggctccgc
     7141 cttcatcgcg tcgtccaaac tcaggggttt gttggcttcc aacggaatca gcttggacag
     7201 tttgtcgcac agcataaacg gcagctccaa cacgcgcccc acgtcgcgga tgaccgcttt
     7261 ggacgacatc gtgccgaagg taacaatctg gctgaccgcc tgagcgccgt atttatcgcg
     7321 cacatattcg atcacgcggc cgcggtttgc ctgacagaag tcaacgtcga agtcgggcat
     7381 ggagacgcgt tcggggttta agaaacgttc aaacagcagc gcgtatttca gcgggtcgag
     7441 gtcggtaatt ttcagcgaat acgccaccag cgaacccgcg cccgaaccgc gtcccggccc
     7501 gaccggacag ccgtgcgttt tcgcccagtt gataaagtct tgtacgataa ggaaatagcc
     7561 ggggaatttc atctggatga tgatgttcag ctcaaaatcc aaacgttcct gatattccgg
     7621 catttttgcc gcacgctccg cctcgtcggg ataaagctga accatgcgtt cctgcaagcc
     7681 cttgtttgag agtttgatga gatagtcatc gcgcgataaa ccgtcgggcg tggggaaaag
     7741 gggcaggaag tttttgccca atgtgatgtg cagattgcag cgtttggcaa tttctaccgt
     7801 gttttccaag gcctcgggca aatcggcgaa acgctccagc atcgtttccg gcggaatgaa
     7861 aaactggccc ggtgtgaaat cgcgcggacg ttttttgtcc gtcagcaccc agccgcccgc
     7921 aatacacacc cgtgcctcgt gcgcgttgaa atcatcgctg ctcataaact gcgtcgggtg
     7981 ggtcgccacc accggcaaac ccagttcctc cgccagcttc acgctgccgg aaacgcaagc
     8041 ctcccattcg gggcgttcgg gcaggcgttg cagctctaaa taaaacgcat cgggaaacca
     8101 tgccgcatat ttcaacgccg ccgcacgcgc cgcgtcttca ttgccgttca acagattcac
     8161 gcccacttcg ccgtaatgtg cgccgctcaa acaaatcaag ccgctgttgt cgccgttttc
     8221 cagccattcg ggattgagtt ccgcatgatg gatattgcgg tctttgccga cataagccgc
     8281 cgtcagaagc tcgctcaagc gcagatagcc cgcatcgttg cggataatca gcatggcgcg
     8341 aaacggcttg tcgggcgcat tcggattgcc tatccacacg tccgccgcgc cgatgggttt
     8401 aatccctgcg ccgcggcagg ctttatagaa tttcaccaag ccgaattcgt tcatcaaatc
     8461 gctgatgccc aaagcaggca aaccgtattc ctgcgctttg gcaaccagtt ttttaatccg
     8521 caccataccg tcggtaattg agaactcggt atgcaggcgc aaggggatgt aggtcggctc
     8581 ggtcatggca aaatcggcgt gaacaataaa aggcgtattg tagcagggtt gtctttagat
     8641 ggcggtgtag gtaatgccgt ttcgggttca gacggcattg cctgcaaatg gttttttgaa
     8701 cttttacgac ggcaaaaaaa tgcctcctgc cgtatggcgg aggcttccca aggagtattg
     8761 atagatataa aggactatca aactagttat aaggaactat ataccttatt cggacggatg
     8821 gcaagcagtt aaattaattt tacgttcaaa caggtttttg atttcgtttt gatgccgatt
     8881 gctggtgtat cgggcagttc gcgtttgagg aggtgcatca gcgtcaatgt ggattcgtcg
     8941 gggaacagga tttgcagcct gccgccggac aggatttctt tgaccggctg gcggcatacg
     9001 gggtttcaga cggcattttt acggaacagg cggtgcggat acatcgctgg ttttgcggta
     9061 attttgggta cggcggcggc aggtgctaca ataacgggct ctttctgcaa gggaacatca
     9121 tggaagccac cgtctatctc gaagacaacg aatacatcgc cctgtgcgac ctcttgaaat
     9181 tggccggact tgccgaaagc ggcggacagg cgaaggcgtt gattgccgaa gggttggtgt
     9241 cgcgcaacgg cgaaaccgaa acccgtaaaa ccgccaaaat acgcggcggc gaagtcatcg
     9301 agtttgacgg tgcgcgcttg gaaatcgccg atggatatga ccctgaagca taaagccgaa
     9361 gcccttttgg gcgagccgct tttagacgaa cccgtccgac ccgagtcgtg ggaatgctgc
     9421 ggcagcgact gcggcgaggc gtgtattcag acgatttatt gggcggataa ggcccgctac
     9481 gacgcgcaac ggcggaaatt gaaagaagcg ggttggtcgg acgatgccgt ctgaaaacgg
     9541 ttcggct
//
LOCUS       DAXHRI010000069         9546 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXHRI010000069 DAXHRI010000000
VERSION     DAXHRI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.069"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(100..312)
                     /locus_tag="ACFM75_001213"
     CDS             complement(100..312)
                     /locus_tag="ACFM75_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6255763.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(324..605)
                     /locus_tag="ACFM75_001214"
     CDS             complement(324..605)
                     /locus_tag="ACFM75_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255764.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(643..846)
                     /locus_tag="ACFM75_001215"
     CDS             complement(643..846)
                     /locus_tag="ACFM75_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255765.1"
                     /translation="MNIIEQETKRAELRKIDAEIAKIIADAHKINAESVKIAQESRWY
                     PMIAATGLVTAIAAVLALIFKFA"
     gene            complement(933..1550)
                     /locus_tag="ACFM75_001216"
     CDS             complement(933..1550)
                     /locus_tag="ACFM75_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFC6255766.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQTPQNKNRLKKIQTASCPKHLQ"
     gene            complement(1525..1869)
                     /locus_tag="ACFM75_001217"
                     /pseudo
     CDS             complement(1525..1869)
                     /locus_tag="ACFM75_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1862..2737)
                     /locus_tag="ACFM75_001218"
     CDS             complement(1862..2737)
                     /locus_tag="ACFM75_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HFC6255767.1"
                     /translation="MSNEVEVMDAVSVQNYQVAHSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2783..3073)
                     /locus_tag="ACFM75_001219"
     CDS             complement(2783..3073)
                     /locus_tag="ACFM75_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6255768.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRRQHERLQSKIAEIGKRVNAD"
     gene            complement(3085..3288)
                     /locus_tag="ACFM75_001220"
     CDS             complement(3085..3288)
                     /locus_tag="ACFM75_001220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6255769.1"
                     /translation="MLSQWERAIYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYL
                     VVGGQRKGNYGKGHEIAKKLGLK"
     gene            3378..3755
                     /locus_tag="ACFM75_001221"
     CDS             3378..3755
                     /locus_tag="ACFM75_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6255770.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            3784..4029
                     /locus_tag="ACFM75_001222"
     CDS             3784..4029
                     /locus_tag="ACFM75_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255771.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4263..5231
                     /locus_tag="ACFM75_001223"
     CDS             4263..5231
                     /locus_tag="ACFM75_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255772.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5228..5710
                     /locus_tag="ACFM75_001224"
     CDS             5228..5710
                     /locus_tag="ACFM75_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255773.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            5800..6303
                     /locus_tag="ACFM75_001225"
     CDS             5800..6303
                     /locus_tag="ACFM75_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC6255774.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGVIGAVSLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            6317..6490
                     /locus_tag="ACFM75_001226"
     CDS             6317..6490
                     /locus_tag="ACFM75_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255775.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            6415..6747
                     /locus_tag="ACFM75_001227"
                     /pseudo
     CDS             6415..6747
                     /locus_tag="ACFM75_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6744..7154
                     /locus_tag="ACFM75_001228"
     CDS             6744..7154
                     /locus_tag="ACFM75_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC6255776.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            7159..7707
                     /locus_tag="ACFM75_001229"
                     /pseudo
     CDS             7159..7707
                     /locus_tag="ACFM75_001229"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7723..7908
                     /locus_tag="ACFM75_001230"
                     /pseudo
     CDS             7723..7908
                     /locus_tag="ACFM75_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(8227..8589)
                     /gene="ssrA"
                     /locus_tag="ACFM75_001231"
     tmRNA           complement(8227..8589)
                     /gene="ssrA"
                     /locus_tag="ACFM75_001231"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8652..9257)
                     /locus_tag="ACFM75_001232"
     CDS             complement(8652..9257)
                     /locus_tag="ACFM75_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC6255777.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2319 a         2182 c         2420 g         2625 t
ORIGIN      
        1 gtgagtttat tttggggcgg cggcaggtct ggggcaagcg gcgtggggct tggttgtggt
       61 ttttaggttt ttgggggtaa aaaatgccgt ctgaactttt cagacggcgt ttgttttttc
      121 tatccaatcg aggaactgcc gccatttttc cagcggcata tcggcccgcc gcgtttcggt
      181 atcgggttcg gcttcccagc ggcctacctg tatgtagtgc ttcacgccga cgatttgcgc
      241 caactcggcc tgcgtcagtt tgcagcggtt gcgcagggtg cgaaggttgt aaggcgtgta
      301 gccgagttcc atgtcgttgc ggtttatttt gtttcgcata tttttttgac tgcccggcgg
      361 caggtttcgg taaggacggc ggcaaatcgg gcttcgtctg ccggtttgcc gtcgaaaaaa
      421 ataaaatcgt aaagggtgat gccgttttcg gaatggcggt aatgcccgcc cgggcattcg
      481 ccgtctgtct gcctgtggat tttggcaatc aggcggggat agcggcaatg ggtaatgaaa
      541 cggcttgcgc cgtcttgccc gataatccat tcggggtagc ggttgaatag tgcggctctg
      601 ttcattttgt tcgtgggata aagcccctcg cggggcttgt ggtcaggcaa atttgaatat
      661 cagtgccaac acggcggcga tggcggtaac cagcccggtg gccgctatca tgggatacca
      721 gcgggactct tgggctattt ttacggattc ggcgttgatt ttgtgcgcgt cggcaatgat
      781 tttggcgatt tcggcatcta tttttcttaa ctcggcgcgt ttcgtttctt gttcgattat
      841 gttcattgga tttcctttcg tttttggcgg ttgctgccgc ttgtcggatg gcaggatgtc
      901 tgccatgtgt gtatattaat accttttagg ttttattgca agtgttttgg gcaggatgcc
      961 gtctgaattt ttttcaggcg gtttttgttt tgcggggttt gggcggcggc ttcgtatgct
     1021 tggcggtggc ggcggctgta ccattcggcg agttcgcgcc gctcttggag gcggtagctg
     1081 tcggcggtca ggtagtggtg caggcttgag aagccggcgc gttggagggc ggcgcggctg
     1141 atgtggccga gggcgaggtc tgttgtgagg gtgtcttttc cggcggtcag gctgatgttg
     1201 gtgaagaggt ggcggaatcc gtgcattgtg tgtttggatt tgccgggggt gctgccgtca
     1261 tagcccagtc gtcggatggc gttgtgggcg aatttgatgc tgatgtggtc gggatggggg
     1321 gcgggtttgc gccgtgggcg gatgcctggg aaaaggtgga tgttgtcgcc ggtctgtgtg
     1381 tgcagctctc ggagtatttc taccgcccag tccgacagtg ggacggtaaa ggggtgtttg
     1441 gtcttcatgt cggcggcggg gatgtgccat aaccgggcgg tgaggtcgat gtcttgccag
     1501 cgggcggaaa gcagtgcgga cggacgggat gacttccccg gtttccccat caatccgcgc
     1561 cgcctcgatt tttccgttgg cgcgcagcat ttccaccgcc tgccgcaccg acatcaaccg
     1621 cttgccgttg cgcctcatcg cctccaccaa aaccgccgaa atcaatttgg cttcttccgg
     1681 tttaagctcc gtcttgcccg catcgctgcg ccgtttgcgc gtcggcttga tgctgaccgc
     1741 ctccagcttg cggtatagcg tggcaaggct gatgcccaat tcctgcgcct gctgcttaag
     1801 atatgcagag cgtgcgccgc gtcccattgc ttccgcctga ttctcgactg ccttaagacg
     1861 ctcaatcatt gccggattca tcgccttctc ccgtttcacc gcccaaccat tccggcacat
     1921 tgtctgtcgg tgcttcagtc ggcagggcat agctttcgcg cagttgctcg cagtccaaaa
     1981 taatttgatt gagcgtgccg accatctttg cctgatggct gatctcgtgt gcctcactgt
     2041 gcgcattaag ttggtcgaac aaatctttca aacggctcac ttgactgcgg ataccgacct
     2101 caaggcttgt taactgcatc gccaactcac tgcccacgtc ttccgccttc ggctctctga
     2161 caacggtttg cttcttagcc agcttctcgg tcagctcatc aattttggct gttttggttt
     2221 tcatcacttc gtctttggcg gcgaggtttt cacggctttc gcgcagggcg acgcgcagct
     2281 cgcgcaccgt cattcggtcc acatcgtcaa aggtcatgcc gttgacttct tctccttcgg
     2341 caagtcccac caacgtaacg tcttcttcga ccaagagttc cagcagcttt gacttgccca
     2401 aatccatcag cttcggcgcg gctttctgca tttgcggggt cgcaaagcgg cgagtggctg
     2461 acatcagacg tgatgtttct gcgataccca gcccaaattg gcttttcaca atttccataa
     2521 accgtccgtg ttccgtatgc tcttttaaaa taatcagcgc acgtcccagt tcgaacatgc
     2581 cttccatcgt ctgccgtact gcttgacgac cgcgttcaac ccaacgctct tcgctataag
     2641 tctcgccgtt accccactgc tccatcacca gcacactgtg cgcgacttgg tagttttgca
     2701 ctgaaaccgc atccatcact tcaacttcat tgctcatttt tcactctcca aatttcctta
     2761 cgtcaggaaa tttcaaaatt cgttaatctg cattcacccg cttgccgatt tcggcaatct
     2821 tgctttgcag ccgttcatgc tgccgcctga accgctcggc gatttgcagg gttttgatac
     2881 cgtaggcgta gttgccgttt tcaagcttga tgaccaatcc cgatgcaacc aaatcatcaa
     2941 tatccctgct gacttgcgat ggcgtcagcc ccagtccgac cgataaatcc ttattgctca
     3001 gaccgataat cggatgctcg tcaagcgcga taaagaccct caatagccgt tgtacccttt
     3061 tactttctgc catccgcatc ctccttattt cagtcccagc ttcttggcaa tttcatgccc
     3121 cttgccgtaa ttgcctttgc gctgtccgcc gaccaccaga tacacatcgc gcggcttaaa
     3181 gccgttctct cttgcccact gcgccagcgt ttggccgttt tgggcaaaat tttcttttag
     3241 tttttcaaca gtatagccat agatagccct ttcccattgt gataaaatag tgaactattt
     3301 atgcactaga aattaattag tgcattacca atgcgcgaat tatgagattt taaaatctct
     3361 tttgcaagga ttattttgtg atttctaaat ctctttttgg taatcgtttg aaagaaaaaa
     3421 gaaagttttt agggcttacc caagcacaag ctgctgaaaa agcaggtatt gagcgcgaaa
     3481 catggggcaa atacgaacgt ggcgtattta tgcctagtgg agatgtattg ctctcttttt
     3541 tgaatatggg aatagatgtg agcagcttat ttgctgctga acagggaatt aggccgtctg
     3601 aaaattctga aattagtaag gaagagtcag agttattagg gtattaccgc caagccagcg
     3661 ataacggcaa atttgtcatt ctcagtgtgg caaaaggcgc agagaaaaaa gcagccgaaa
     3721 atactcaagt ttcagccacg cataaaacag cgtgattttt aaatccaaaa tggagacagt
     3781 cttatgtttg actttgaaca acaaatcaaa tggggtgagc gagcggaaga aatcgttaaa
     3841 gaggcagcca cccagaacaa tatcgaaatt cccgaacctt tggcttcggc attagctaag
     3901 gcggtaaaag tgcattatct aagccaagct ggtgtgtttt ctttggtaga agcctatgca
     3961 gacacggtaa atccaactga aaaagaagta gattaccaag caatcggcaa agagctattt
     4021 gaaaaataaa aaatgccgtc tgaaattttc agacggcatt taggacactc cgacagtcgg
     4081 gctgctccgg tgttctgaaa cggatattaa gcaattgaag cggccgtgca ttttaaaacg
     4141 cattaaaaac taccccgaca tacaggtcat cgggcaggtg gttcaggtgt tgaaagattt
     4201 gaattgatgc cgtctgaatt tattttttcg acacagatat taaaagagaa aggactgtac
     4261 aaatggcaaa aaaaactact aaaactattc attatttacg tgcgcagcaa aaccatagct
     4321 tgttcgattt agaggtagca ttaagaaggg ttttgtcagc tgctcctact gttcaagata
     4381 cacaaattga acaatacaat cagcttaccc aaatcatgca tagaactttg gatcctaaag
     4441 atgtaaaagg caattcaatt ggtggattac tgatacacat tggcagcggt acaaaagatg
     4501 aacatatccg cactatgagc aatagacctg tacagcaaga tgatcatggt ggaacacaag
     4561 ctcctccaag tggatattcc tttttgcgta aagaggcttt tttgtatatt gttggacacc
     4621 atgtaatttt ttgcggacat ggttttttgt cagcatcaac ggttgcttct tatttaagtt
     4681 tacttagcaa taaattgaga gagaacaatc cggatattgt tcaatttaat attgaattca
     4741 aagcagtagg caattgcgac aagttgtcat tgatacaaca acatggggta aaaagtattc
     4801 ttttagatgc ttcggcatat caattatcca gaaacagact gtatcaagat agccgttcaa
     4861 ccattgctaa agcattaggg aaagttggta gtgtatttac aagcgaattg agtgatgagg
     4921 aattggaagc acaatccgaa attcatatca atttagaagt cttgctaaac ggtaattcga
     4981 gagccagtat tgaagcgcaa tcgctgatgc aggagcaggc agaggaaatt attgatgatg
     5041 aaacggttaa tcaaggattt tccattacca cgcaacaagg tgaagtgatt cagccctctg
     5101 atgtaaaact gtctaaatct gttagaatag gacgatacga tgaagccaac tcgttactcc
     5161 ctgactcggc atttacagca atcagtgaat atttcctaga actccaaagc agaaacttaa
     5221 ctgaacaatg aaaaaatatc tgcccttact acgttttttg ttgattgctt gtatatcagc
     5281 atttttagca tggaaagggc aaccttttgt tcatgggaac gagaaggccg ttgatttaat
     5341 tattaacgta tttgcaatct tagccggttt tttaattgcc attatgacat tgtttagtga
     5401 tatgaggttt gatgaagatg caaattggcg acaaattcaa attcgtgaag gcgtacaaga
     5461 acaacggtat ataaaacatt ccttactttt ttacacatat cttgccgtat tagtttgtgt
     5521 ttttattgtc atcttgttgg ctcataaaga agaatacaaa aatggaccag ccattttttg
     5581 gcttgaacgg agctatttat ttttagcctg tatttccatt ttttattctg tattcttacc
     5641 tgggaattta attaaaagcc gaaaagaaga gtttaaaaaa ttaatggaaa agaagaaacc
     5701 caaaatctag aacgagtttt aacccccttt aaaatcccat tcagacgacc ttttctaaaa
     5761 tccctgtatt gatttttaat tcaatacagg gatttttcca tgtcagacaa gttcaaccaa
     5821 ttcatcaacc gcgtcctctc tcacgagggt ggttacgcca accatcccaa agaccccggc
     5881 ggcgaaacca attggggcat cactaagcgc accgcacagg caaacggcta caacggctcc
     5941 atgcgtgcca tgacgcgtga acaggcaatc agcatttacc gtaaagcgtt ttgggagcgt
     6001 taccgcgccg accaaatgcc ggaagcggtc gcgttccaat tttttgatgc ctgcgtcaac
     6061 cacggttacg gcaatgccgc ccgtatgctc caacgcgccg caggcgtacc ggacgacggc
     6121 gttatcggag cagtcagcct caaagccatc aattcccttc ccgaaaacga ccttttattg
     6181 cggttcaacg ccgagcgtct ggtcttttat accaagctcg gtacgttcac ctctttcggc
     6241 aagggctggg tacgccgtgt ggcgcaaaac ctgattcacg cgtctgcaga taacactgat
     6301 taaagggaga taaaccatgt caaaaaagtc actcatcgcc ctaatgaccg cagccatgca
     6361 gcccgatttc agccacagcg acctaggcat tcgctacgcc atgccgactc agggatgttg
     6421 gacgcaagcc caccgcaaga gcggggtagc cgccgcgaaa cgcgcagcca aaaaaaagcg
     6481 tcacaaataa ccgccttttt ccgatggctg ggcggcttgg tctctaatcc ggccacagga
     6541 aaaatcagcc ataccaaact atgggcaaac gttgccgcag ccgccatgac ttggaagttc
     6601 gtgcaggcgg cggacgcgcc cgaatggctc tagtgggctt atggcgcatt ggtcggcggg
     6661 tatgcattaa tcaaacgcgg catcgcggcg attccgcagt tggcggaagt caaaaaatcc
     6721 gcaaatcagg aaggggggcg gcaatgattg aatttgtccg agccaaaaaa cggctgcttt
     6781 gggcatttgt gcttttgctt gtgtggacgt gcggttaccg atacgccgcc gacaaggccg
     6841 aagcggaaca aaccgccctg attgccacct atcggcattc ttctatggtt gcggcggaac
     6901 aatatgcctt gcagcttaaa aaagcgcagg acgaaaggca gcggtggtac gacttttccc
     6961 aaaaacaagg aagaaagccc gtgaaaaaac agtatccgcc gcaaacgaaa aaagccggct
     7021 atctgaaaac caaggaagaa ctgcttgcgg aattggcttg ccttacagcg gaaatggctg
     7081 ccctaaaaaa gcccgatgcc ttaatccatg ggaaagaagt gcggcagaaa gaacgcaact
     7141 cgtcgcaggg ttaaggcaat gccatccgtt gaactgctgt tggagattgt cggactgcca
     7201 cgcggcacct tttattacca attggccgtc caatcggcag aagacaaata tgccgatttg
     7261 aaacagcata tccatgatat ttatcgacga cataagggaa gatacggcta ccggaggatt
     7321 gcagcagcca tccttcatgc aggaacaccg gtcaatcaca agaaagtcag ccgtctgatg
     7381 gcgaagacgg ggctgaaggc agtgatacgg cggcgcaaat accgctcgtt caaaggagaa
     7441 gtcggcaaaa ttgcgccgaa tatcctgcga cgctgtttcc atgcagaaaa gccgaatgag
     7501 aaatgggtaa cggacgttac cgagttcaat gtaggcggag aaaagatata cctttctccg
     7561 attatggatt tgtttaacgg ggaaatcgtc agttaccgta ttcaaatccg cccgactttc
     7621 gatttggccg gcgagatact gaaaggtgcg ccggagaaac cgggatcgtc tgaaaagccg
     7681 atactgcatt cggatcaagg ttggcaatat cagatgtttt tttatagtgg attaaattta
     7741 aatcaggaca aggcgatgaa gccgcagaca gtacaaatag tacggcaagg cgaggcaacg
     7801 ccgtactggt ttaaatttaa tccactatac aacaacgata gaatcaagtt gaaattaaaa
     7861 gggctgagcc ttgttcagta cagaattcag tccctgaaag ccgcttgatt aaactgtccg
     7921 actttttggg gtcagttcgg cttcggcatt tttttatccg tttgggggta acttgtttgg
     7981 aaagctgcaa gcttataaat aaaggattac atttaagttt tgggtaacct ttttaaaaaa
     8041 atgcgtgatg acttttgcat ttttaaggcg ttttttgggg taattcgtga aaagttaccc
     8101 caaaagttac cccataaatg gcgaaaactc aagcatacgc cagcatattc aaggcaacaa
     8161 aaaagccttg aaactgttga agttcaaggc ttttttgtgt tgcaggatgc tgctggaaat
     8221 agggtatggt ggaggcgggg ggaatcgaac ccccgtccga aagtcctcta caaagcgttc
     8281 tacatactta gttgtgtcta tttgaaaatc ttatttccat catgccgacc aacaggcctt
     8341 ttggaaacca gttaccttaa atctcatttc ctgccaagta acccggtagg aaaccagtca
     8401 atgtaagatg acgttgcggt ggctttcgcc acacagccca ttgaccgact gctgcaacgg
     8461 ctagccttaa gcggctaaag cgtaagtttc gtcgtttgcg actatttgaa ttcagtgttt
     8521 tacgggaatc tgagaccccg gtatgcccgc atctgcttcg caacccccgt cgaaaccaag
     8581 gtcgccccca gaaatggttt gcaaattata cggatattgt gcggtgctgc caagtctgtc
     8641 ggagaaattt gtcagtcttg ctgccttaat ttgcgtttga gcaggatgcg gacgcagccg
     8701 tcgttgcctt cccggggttc ggcgtaggcg agtacgtcgg ggtgttgcat cagccagttt
     8761 cgggtcatat ttttcagaac gggtttgtag cctttggaac ctaatccgct gccgtggatg
     8821 atttcgccgc atacgccgcg tttttgggtg aatgcgatga attcgttgag gactttttgg
     8881 gcttcttcct gtgtgtagcc gtgcaggtcg acatcggtaa cgacgggata gtatccgttt
     8941 ttcaggcgtt ggatgtcgtt ttttccctgt ccgtttttgc tgaagctggc gggcgggtcg
     9001 ttgtatgtgc tgccgatgta gaagtagttt tcttcgtcgg cgcggttgtc tttgggacgg
     9061 actttgatgg gggttttgtc gggcggcgca taatattgct gccggttttt taatggggag
     9121 acttgtccga ctgcttgtga aaaatcgaaa tcctgttctt gtttttgctt gtttttttcg
     9181 gctgcctgtt tttctgccgc ttctttttgg gcttgtttgc ccagttgttt gaggatgttt
     9241 tggaagtcgg tattcatatt ttttcctgtt atttgtccga tggctgtttc gggcggggtt
     9301 ttaatttgcc ggaatgtttg ccaatcgggg gaggatgatt ttgttgcctg cgtatgtttt
     9361 ttgaaagtgt gattgtatat caaaaagaaa tgcggcaacc gtcggcagtg ttgattgccg
     9421 gaaatgcgga ccggtcgaac cgatatgccc gaacgcctga taaagtttta aaaacctgcc
     9481 ttgcgaagca ggctgacgtg ttttgccaat cttgaattgc cggaaacgcg aaacacggaa
     9541 atctga
//
LOCUS       DAXHRI010000070         9530 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXHRI010000070 DAXHRI010000000
VERSION     DAXHRI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.070"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(235..1677)
                     /gene="aldA"
                     /locus_tag="ACFM75_001233"
     CDS             complement(235..1677)
                     /gene="aldA"
                     /locus_tag="ACFM75_001233"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC6255778.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1712..1912)
                     /locus_tag="ACFM75_001234"
     CDS             complement(1712..1912)
                     /locus_tag="ACFM75_001234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255779.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1921..2310)
                     /locus_tag="ACFM75_001235"
     CDS             complement(1921..2310)
                     /locus_tag="ACFM75_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255780.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2519..3436)
                     /locus_tag="ACFM75_001236"
     CDS             complement(2519..3436)
                     /locus_tag="ACFM75_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6255781.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRSRFQIDADGGKVILISVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWGLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNEGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3609..4409
                     /locus_tag="ACFM75_001237"
     CDS             3609..4409
                     /locus_tag="ACFM75_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6255782.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4457..5233
                     /gene="mlaE"
                     /locus_tag="ACFM75_001238"
     CDS             4457..5233
                     /gene="mlaE"
                     /locus_tag="ACFM75_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC6255783.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5284..5778
                     /gene="mlaD"
                     /locus_tag="ACFM75_001239"
     CDS             5284..5778
                     /gene="mlaD"
                     /locus_tag="ACFM75_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC6255784.1"
                     /translation="MKKNILEFWVGLFVLLGVAAVGFLAFRVAGGAAFGNSGKTYTVY
                     ADFSDIGGLKANAPIKSAGVLVGRVGSIELDPKSYQAKVRLDLDSKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5815..6405
                     /locus_tag="ACFM75_001240"
     CDS             5815..6405
                     /locus_tag="ACFM75_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC6255785.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDTQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            6456..6734
                     /locus_tag="ACFM75_001241"
     CDS             6456..6734
                     /locus_tag="ACFM75_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC6255786.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6731..7558
                     /locus_tag="ACFM75_001242"
     CDS             6731..7558
                     /locus_tag="ACFM75_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC6255787.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7683..8045
                     /locus_tag="ACFM75_001243"
     CDS             7683..8045
                     /locus_tag="ACFM75_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC6255788.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            8045..8428
                     /locus_tag="ACFM75_001244"
     CDS             8045..8428
                     /locus_tag="ACFM75_001244"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6255789.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            8425..8913
                     /locus_tag="ACFM75_001245"
     CDS             8425..8913
                     /locus_tag="ACFM75_001245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC6255790.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANYYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8996..9124)
                     /locus_tag="ACFM75_001246"
     CDS             complement(8996..9124)
                     /locus_tag="ACFM75_001246"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC6255791.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(9130..9510)
                     /locus_tag="ACFM75_001247"
     CDS             complement(9130..9510)
                     /locus_tag="ACFM75_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC6255792.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     ERGVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFNRS
                     KNALRATRKSVRLRWRTMSGRGTG"
BASE COUNT         2209 a         2652 c         2385 g         2284 t
ORIGIN      
        1 gtacggcgtt gcctcgactt agctcaaaga aacgattctc taaggtgctc aagcaccgag
       61 tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt ttaaatttaa tccgctatac
      121 aaagacaaaa acatcgatgt cggtcatgac tgattgctgg tgaaactgta aaaatgccgt
      181 ctgaacctga aaacgggttc agacggcatt ttctatcggg gttttaaagc ggcattaaat
      241 gtcggtttcc agataaacga cttgggtttg cagatattct tccaaaccgt gtttgccgtc
      301 cgcgccgccg ataccggatt ttttccaacc ggcgtggaaa ccctgcatcg cttcaaagtt
      361 ttcgcggttg atgtaggttt cgccgaattg caggcggcgg gtaacgtaga aggcttcgtt
      421 taaattagtc gtataaacag aactggtcag accaaactcg caatcgttag ccagtgcgat
      481 gacctggtcg agcgtgtcga aagcggaaac gggcagcacg gggccgaagg tttcttcttt
      541 cataatgtcc atactgttgt cggtgtcggt cagcagggtc ggctcgaaga aataaccgcg
      601 tccttcggcg cgtttgccgc cgcaaaccaa tttcgcacct tgtttgactg cccgttccac
      661 tttttcggca acggctttga cggcgcgctc ttcaatcagc gggcccattt ccagcgcgcc
      721 tgcttcggct tcggcagggt tgccgtagcg tacgcctttc atggcggcgg tcattttttc
      781 aatgaatgcg tctttcagac tgctgtggac ataaacgcgc tcggcgcagt tgcagatttg
      841 accagtgttg ccgacgcgcg aagccaagat ggatttcact gccaagtcca aatccgcgtc
      901 tttcaaaacg atagcaggtg ctttgccgcc gagttccagc gagactttgg tgatgttggc
      961 agaagcggct tccatcactt ggcggcccgc ttcaacggag ccggtcaggc tgaccatatc
     1021 gacttgcgga tgggcggaca aggcattgcc gatttccgca cccggaccgt tcacaacgtt
     1081 gaacacgcct gctggcagtc cgaccgcatc gacgatttcg gcgaagatgt ggcagttgat
     1141 cggggttacg ctgctgggtt tgacgacgat ggtgttgccc gttaccaaag cggggcccat
     1201 tttgcgggca atcaggaaga aggggaagtt ccacggcaaa atgccggcga ttacgcccag
     1261 cggacgtttg aacaataaaa tattttcgcg cgggcggtcg ctttggatga tttcgccttc
     1321 gtaacgacgc gcccattcgg cttggtaatc gagatagtcg gcggtaaaca tgacttccac
     1381 gcgcgccaag tctttggttt tgccgccttc ggcaacgatg gtgtcggtca gctcgtcggc
     1441 gcgtccgcgt atgccttggg cgattttgcg caaatacgcg ccgcgttcga ccgcaggcag
     1501 gcgttcccaa gccggttgcg ccgcgcgtgc cgccgccaca gctcggtcaa cgtctgcttt
     1561 tccgcctttg ggttcgcggg cgatggtttc ttcggtggat gggttcaata cgtcgcgcca
     1621 ttcgccgttg aaatcgtttt caaagcgtcc gttgatgtac atggccaatt gtttcatttc
     1681 gggttctcca gttttgtagt cagatgtagt cttagtttat tcccaaataa attgccgtac
     1741 aagtttcttt tgaaatgttt agacgagaag ctactatgtc tgcatattgt tctgctaatt
     1801 tatattcact cgaatgcaac tgccgattat tccaatctgc attcgcatca gtaaaagccc
     1861 cagcattgcc gcctgtggca taaccaatcg ctaaactgcc aacggtatcc acaacaggtt
     1921 tcaggctgcc tgaatttctc taataaatga aacacttatt ctatcagccg ttaatgtaac
     1981 caaacaatta tcaagataag agtagtttac taaatactca tcttcttcaa attgtatatt
     2041 ttgtagggtt agatgattat tttttaattc tgccgttatt tcatacttga aactatcatc
     2101 tatttttcta aatccaatat ttgaagttga tttatccagt atgtaatcat taaagatttc
     2161 ataggataat tccacttcat aaaattgatt ttcaaatatt ttgaatgggc agtatgatat
     2221 aaaacaatgt agttcataac catttatttc aatcaatact gcctcttcaa tggtgttatc
     2281 taactttttg attttacatt tatataacat tttatcttgc ctttagataa attgttttgt
     2341 tttttggatt gttattcaaa gttctccaaa agtcttttcc tgattttgta tgtatcgtgg
     2401 taacactacc attttcattg gttccaacaa aaataaattt agattgacct ttggaatcag
     2461 tacccaaaaa ctgaacatat ccagacatgc agatttcggg ttttaaatca aatgcggctt
     2521 accggttttc ttttttggtt tccgccggcg tgctgccgaa atattgcctg aatgcctgag
     2581 taaagctgga aacgtggcgg tagccgcata aatatgcggt ttcgcctatg cttttcccct
     2641 cgttttgcaa caggtagagc gcgtgctgca tttgtttgtg gtgcagccac tcgcttgcgg
     2701 taataccgaa atggtcgcgc atacggcgtt gcagcgtcct ttcactgatg ttcagcgcgt
     2761 ctgtcagccg gttgacttgg tgtgcgccgt cggcaaacgc ggcatttagg gtgcggctga
     2821 agtcttcaga cggcatagcg tctgcttccg ccgtttgccc cgccgccggc ccgatgccgt
     2881 ctgaaaccgt gtcccacaag tccgacagca gccgcaacac gtccgcctcg cggcgcaatg
     2941 tttcgcccaa atgccccttt gggacggctt tcaggcagga tgccgccaag ccgcgcaggt
     3001 tcgggggcaa accccatatc ctgaccggtt cgcggtaaag caggggtgcg aaacgcgcgt
     3061 attccggacg cagcagccat tgttccatac ctttaatggt cattttgacc gttttgccgc
     3121 cgcgataaag atagcggctg aacaggactt cttccccgac tgaaatcagg atgaccttgc
     3181 cgccgtccgc atcgatttgg aatcggctcc ggttgatgcc gaagtccaaa ctgccttcca
     3241 gcaagagcac gaacgacaca taaggttcgg cgaggcggct gctgcaaaaa tcacaccgtg
     3301 ccgttactgt gccgccgtgg atggaaatgc cgttggacaa agtatcgaaa cggtagctgc
     3361 cctccacata gctgccgccg ccgcttttga ctagattgac gaaccggctg ctgttcaaat
     3421 gttctgcttt ttccatagtt ttctgataaa ggttatcatt tgaaagataa catttttcgc
     3481 cacagcaaac aatccgccgc cgacaacggc attttcacaa catttaccgt ccgtatgccg
     3541 tctgaaaatt gcccgtcttc ccgataagcc ttaaaataca cgccgtcaaa cctaccgtcc
     3601 gtccgtacat gagcccatcc ccctttatcg aaatgaaaga cgtcgccttc gcgtatggcg
     3661 accgcccgat tctgaagaac atcaatttca gcattccgca aggcaatttt gctgccgtaa
     3721 tgggcggttc gggcagcggc aaaaccacgc tgatgcgact gattacagga cagattcgtc
     3781 cgcagtccgg gcaggttttg attgaaggac gggatttggc gggcttttcg gctgacgagt
     3841 tgtatgaaca ccgccgccgt atgggcgtat tgttccaaca cggcgcgctg tttactgatt
     3901 tgtcggtatt cgacaatatc gcttttccga tgcgcgaact gacgcgcctg ccggaagcgg
     3961 tgattcggga tttggttttg ttgaaattga acgcggtcgg tctgcgcggt gtggaaaacc
     4021 tgatgccgtc cgagttgtcc ggcgggatgt cgcgccgcgt cgcgcttgcc cgcacgattg
     4081 cgctcgaccc tgaaattatg ttgtacgacg agccgtttac cggcctcgat ccgatttcct
     4141 tgggcgtgat tgcccacttg atcagccgcg tcaacaaggc tttgcgttcg accagtatta
     4201 tggtaacgca cgacattgaa aaatctttgg aaatcgtcga tcaggtgatt ttcttggcgc
     4261 acggcgaaat tatgttctcc ggctcgccgc aggaaatgcg cgaactggat tcgccttggg
     4321 tgcgccagtt tgtcggcggg ctggcagacg gccccgtagc ataccgttat ccggcgcaaa
     4381 cgtcgttgca gcaggacctg ttgggataaa tgagttgatg ccgtctgaaa ccgttgaact
     4441 ttacacatag aaatttatga actttatccg ttccgtcggg gcgaaaaccc tcggccttat
     4501 tcaatccctc ggcagtatca cgctgtttct gctgaacatt ctggcgaagt ccggtacggc
     4561 tttcgtccgt ccgcgcctga gcgtgcgcca agtgtatttc gccggcgtgc tgtcggtgct
     4621 gattgttgcc gtttcggggc tgttcgtcgg catggtcttg ggtttgcagg gctatacgca
     4681 gttgtcgaaa ttcaaatccg ccgatatttt gggctatatg gtcgcggctt cgctattgcg
     4741 cgagctgggt ccggtattgg cagcgatttt gtttgccagc agcgcgggcg gtgcgatgac
     4801 cagcgaaatc ggtctgatga aaacgaccga acagctcgaa gcgatgaatg tgatggcggt
     4861 aaaccctgtc gcccgcgtgg ttgcgccgcg cttttgggcg ggcgtgtttt ccatgccgct
     4921 tttggcttcg attttcaacg tggcgggcat tttcggtgcg tatttggtcg gtgtaagctg
     4981 gctgggcttg gacagcggca ttttctggtc gcaaatgcag aacaacatca cgatacatta
     5041 cgatgtaatc aacggtctga ttaaatccgc cgcgttcggc gtggcggtaa cgctgattgc
     5101 cgtgcatcag ggcttccatt gcgtcccgac ttcggaaggc attttgcgcg ccagcacgcg
     5161 cacggtggtt tcgtccgccc tgacgatttt ggcggtcgat tttatattga ccgcgtggat
     5221 gtttacagat tgacaggccg tctgaagacg aaacgcggaa catcggatat tcaaggaact
     5281 ttaatgaaaa agaacatatt ggaattttgg gtcggactgt ttgtcctgct cggcgtggca
     5341 gcggtcggct ttctcgcttt ccgcgtggca ggcggcgcgg ccttcggcaa ttccggcaag
     5401 acttataccg tatatgccga tttcagcgac atcggcggac tgaaggccaa cgcgccgatc
     5461 aaatccgcag gcgtattggt cggacgcgtt ggttccatcg agcttgaccc gaaatcctat
     5521 caggcgaaag tccgtctcga tttggacagc aaatatcagt tcagtagcga cgtttccgca
     5581 caaatcctga cttcgggact tttgggcgag cagtacatcg ggctgcagca gggcggcgac
     5641 acggaaaacc ttgctgccgg cgacaccatc tccgtaacca gttctgcaat ggttctggaa
     5701 aaccttatcg gcaaattcat gacgagtttt gccgagaaaa atgccgacgg cggcaatgcg
     5761 gaaaaagccg ccgaataaac gtatgccatc atattcaaac taggacactt aatcatgaaa
     5821 aaatcctccc tcatcagcgc attgggcatc ggtattttga gcatcggcat ggcatttgcc
     5881 gcccctgccg acgcggtaag ccaaatccgt caaaacgcca ctcaagtatt gagcatctta
     5941 aaaaacggcg atgccaacac cgcccgccaa aaagccgaag cctatgcgat tccctatttc
     6001 gatttccaac gtatgaccgc attggcggtc ggcaaccctt ggcgcaccgc gtccgacacg
     6061 caaaaacaag cgttggccaa agaatttcaa accctgctga tccgcaccta ttccggcacg
     6121 atgctgaaat taaaaaacgc caacgtcaac gtcaaagaca atcccatcgt caataaaggc
     6181 ggcaaagaaa tcatcgtccg cgccgaagtc ggcgtacccg ggcaaaaacc cgtcaacatg
     6241 gacttcacca cctaccaaag cggcggtaaa taccgtacct acaacgtcgc catcgaaggc
     6301 gcgagcctgg ttaccgtgta ccgcaaccaa ttcggcgaaa ttatcaaagc gaaaggcgtg
     6361 gacggactga ttgccgagtt gaaagccaaa aacggcggca aataaccgac aaaccatacc
     6421 gtccgagggc ggtatgggtt ttccgagcaa acgctatgca tacagaactc aaaaacggaa
     6481 tattgcacat cggcggcgac atcaccgtca aaaccctgac cgccgccgct tttacccgct
     6541 tcagacagca atgtcgtctg aaagacacct gcgcggttga tttgagcggc gtcggacgcg
     6601 ccgattccgc ctgcgtgtcg ctgctgctcg aagtgctgcg cggttgcaaa ggcagcgtca
     6661 ggctgaccgg cattcccgaa tccgtgcgcg cgctgtccga actgtacgaa atcaaagact
     6721 ggctgaaatc atgaaaaaaa ccgcctatgc cttcctcctg ctgatcgggt tcgcttccgc
     6781 ccctgcattt gccgaaaccc gccccgccga cccttatgaa ggctacaacc gcgccgtttt
     6841 caaattcaac gaccaagccg accgctacat tttcgcccct gccgcgcgcg gctaccgcaa
     6901 agttgcgccg aaacccgtcc gcgccggcgt gtccaatttt tttaacaacc tgtgcgacgt
     6961 ggtcagcttc ggtagcaata tcttgcgctt ggacatcaaa cgcgcaagcg aagaccttgt
     7021 ccgcgtcggc atcaacacca ctttcggttt gggcgggctt atcgacatcg ccggcgcggg
     7081 cggcattccc gacaataaaa acaccttggg cgacacgttt gcttcgtggg gatggaaaaa
     7141 cagcaattat ttcgtgttgc ccgtcttagg gccgtccacc gtccgcgacg cgctcggcac
     7201 gggtattacc tccgtttatt cgcccaagaa tatcgtcttc cgcacccctg tcggacgctg
     7261 gggcacgact gccgtatccg ccgtcagtac gcgcgaaggt ctgctcgatt tgaccgacag
     7321 tctggacgaa gccgccatcg acaaatacag ctacacgcgc gacctctata tgaaagtccg
     7381 tgcgcggcag accggtgcaa cacctgccga aggtacggaa gataacatcg acatcgacga
     7441 attggtcgaa agtgccgaaa ccggcgcggc ggaaactgcc gttcaagaag attccgtatc
     7501 cgaaacacag gcagaagcag caggggaagc cgaaacgcaa cctggaacac aaccttaaag
     7561 ccatgccgtc tgaaaatccg tgttcggacg gcattccctt ctgtcggaac ggacagtccg
     7621 aaaccacaga ttgggctata atctcttttt tgcgcatacc gcgcgacagc aaaggaaaaa
     7681 ccatgtacga agtcaaccgc agcgtattcg tcctcatccc tttagaacct ttttggaact
     7741 ggctgcaaac cctgccgggc aaccacctcg acggactgac tcttgaagac atccaagccg
     7801 acgccaattc ctaccttgtc cgcccgtgcg aaaccgccga cgaagtatgg gacgaaatcg
     7861 aagcccgttt tgaagacatt ttcgccgccg aacttgccga ctggtgcgaa gacgaacgcg
     7921 agtggcctgc gctcgatgcc gacattttca acgaatggtt cgacatccag ctttctaccg
     7981 tcatcaccga cctcgaacac gaaccgcttg cccgcgaagc cttccaaccc atcaacctga
     8041 actgatgaag ctgaccgtcc gcaactacca tctcgacggc tacggccatg tcaacaatgc
     8101 gcgctacctc gaatttttcg aagaagcgcg ctgggcgttt tttgaagaac gcggactgct
     8161 gcacgagttg gcaggcctca tactgattgt cgcccgcatc gacatccgat acagccgccc
     8221 cgccgtcgaa ggcgacgtat tgcagttttc atgccgcctg aaaacccccg gcacgcgccg
     8281 tattgtgctg acccagacga ttacactgcc aaacggcaaa accgccgcag aagccgacat
     8341 caccctgatg cccgtccacg ccgccacaca acgcaccgtc agcctgcccg ccacccttgc
     8401 ccgcgcattg gaagccttgt ccgaatgaaa aaaatactca ccgccgccgt cgtcgcactg
     8461 atcggcatcc tccttgccat cgtcctcatc cccgacagca aaaccgcgcc cgccttctcc
     8521 ctgcccgacc tgcacggaaa aaccgtttcc aacgccgacc tgcaaggcaa agtaaccctg
     8581 attaattttt ggtttccctc ctgtccgggt tgtgtgagcg aaatgcccaa aatcattaaa
     8641 acggcaaatt actataaaaa caaaaacttc caagtccttg ccgttgcaca acctatcgat
     8701 ccgatagaaa gcgtccgcca atatgtcaaa gactacggtt tgccgtttac cgtcatgtat
     8761 gatgcggaca aagctgtcgg acaggcgttc ggcacacagg tttatccgac ttccgtcctt
     8821 atcggcaaaa aaggcgaaat cctcaaaact tatgtcggcg aacccgattt cggcaaactc
     8881 taccaagaaa tcgataccgc gctggcacaa tagcgatgcc gtctgaagcg caaaaaaaac
     8941 agaaacgatg ttcctgtttt ttcagatcat ccgccccgac acgttcagac ggcattcaac
     9001 cttccggcag attctttttc accactgccg gattgcctgc cgccaaagag tagggcggaa
     9061 tgtcttttgt aaccaccgcg cctgcgccca cgaccgaacc gcgtccgacg gttacgcccg
     9121 ccattacaat caccctgtgc cccggccaga catcgtcctc caacgtaatc ggacggattt
     9181 ccgtgtagcc ctcaaagcgt tttttgaacg gttaaacttg tggttatttg aataaaacag
     9241 acattccggc tccatcatca catttttgcc gaccaccggc ccacggcaga tttcacagtt
     9301 tgccccgatg cccgagccgt cgcccaaaac cgtatccgga aacacatacg ccccgcgttc
     9361 gatattgacc ccgcgttcga tatgcggaga aacccgccgc gccaaaaaac cgcgcacccg
     9421 tctgccgacg atgccgatgc cgcgcatatg tgacggcggc aaccccgaac cggcaaaacg
     9481 cagcagcagg aaaaagaatt ttgatcccat caaacctccc tcaaaagaac
//
LOCUS       DAXHRI010000071         9522 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXHRI010000071 DAXHRI010000000
VERSION     DAXHRI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.071"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..297)
                     /locus_tag="ACFM75_001248"
     CDS             complement(<1..297)
                     /locus_tag="ACFM75_001248"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6255793.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="ACFM75_001249"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="ACFM75_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC6255794.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1662..4877)
                     /gene="carB"
                     /locus_tag="ACFM75_001250"
     CDS             complement(1662..4877)
                     /gene="carB"
                     /locus_tag="ACFM75_001250"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC6255795.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRIAQLLGVKEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4906..5547)
                     /locus_tag="ACFM75_001251"
     CDS             complement(4906..5547)
                     /locus_tag="ACFM75_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFC6255796.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            complement(6320..6487)
                     /locus_tag="ACFM75_001252"
     CDS             complement(6320..6487)
                     /locus_tag="ACFM75_001252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255797.1"
                     /translation="MADFDWIIENNRIKPTELSIYKGRLKNKKQPAQPVFPAEPLIPT
                     AAPSSLPVGKR"
     gene            complement(6491..6880)
                     /locus_tag="ACFM75_001253"
     CDS             complement(6491..6880)
                     /locus_tag="ACFM75_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC6255798.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7454..7579)
                     /locus_tag="ACFM75_001254"
                     /pseudo
     CDS             complement(7454..7579)
                     /locus_tag="ACFM75_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7625..8758)
                     /gene="carA"
                     /locus_tag="ACFM75_001255"
     CDS             complement(7625..8758)
                     /gene="carA"
                     /locus_tag="ACFM75_001255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC6255799.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2139 a         2448 c         2483 g         2452 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacagcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcttt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgttccaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcgcaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc
      841 gatggtgctg catctgctgg cgccgctggc aacaaaattc aacgaacgct atccgcatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctac
     1081 agaagagctt gccggccacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 cgataaaaca caccccttta atgctgttta ttacagcgat aaagccgtca acctccgctt
     1381 gcgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg ccataaaatt gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gccggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgacaccgt
     1621 aaaaaaaggt ttcagacggc attttcaacc tgattcaggc atcagttgcg gtttttcaaa
     1681 cgcccgtgca actcttgaac gctgtacacg cccagatggt ctcggctttt cgcaccttcg
     1741 ctcatcgctt cgccgccggc agtggtggtg tattgcggca cacgttgctg caatgcgctt
     1801 tggcggatga tgtggctgtc ggacacggat tgcggatcgc tggaaacggt attcacgacc
     1861 agtgcgattt cgccgttttt cagcgcgtcg ccgatgtgcg ggcggccttc ggggacttta
     1921 ttgatggttt gtacaatcag cccgtgttcg gtcaggtatt gcgccgtgcc gcgcgtggcg
     1981 cagatgccgt agcctaaggc ttggaagttt ttggcggttt taatgacgcg ttctttgtct
     2041 tcttcgcgca cggagaggaa gattttgccg gtcgggttga ggcgttcgcc cgcgccgagt
     2101 tgggctttgt agtaggcttc gccaaagctt gcgcccacgc ccatgacttc gccggtggag
     2161 cgcatttccg gtcccaaaat cgtatccacg cccgggaatt tgatgaatgg gaacacggct
     2221 tctttaacgg catagaaatc ggggacaact tctttttcca cgccttgttc tttcagggaa
     2281 atgcctgcca tgcagcgcgc gccgactttg gcgagcggca cgccggtggc tttggagacg
     2341 aagggcacgg tgcggctggc gcgcgggttc acttccaata cgaacaccac gccgtcttgt
     2401 acggcaaact gcacgttcat cagtccgacc acgcccagcg cgtacgccat cgctttagtt
     2461 tggcggcgga tttcgtcttg gatttcttcg cttaaggagt agggcggcag cgagcagccg
     2521 gagtcgccgg agtggatgcc cgcctgttcg acgtgctgca tgatgccgcc gataaccacg
     2581 tctttgccgt ctgaaacgca gtccacatcc acttcaatcg cgttgttgag gaagaaatcg
     2641 agcaacacgg ggctgtcttc ggaaacctgc acggcttcgc gcatgtattt ttgcaaggct
     2701 tcggcagagt ggacaatctg catcgcgcgt ccgcccaaaa cataagacgg gcgcacgacc
     2761 agcggatagc cgatttcttc ggctttgacg agtgcttctt cttcgttgtg ggcgatgcgg
     2821 ttgggcggtt ggcgcagacc taagtcgttc aacacttttt ggaagcgttc gcggtcttcg
     2881 gcggcgtcga tgctgtcggc ggacgtgccg atgatgttta cgccgttttc aaccagcgcg
     2941 ttggcgagtt tgagcggggt ttgaccgccg taatgcacaa tcacgcccca cgggttttcg
     3001 gtgcggacga tttccaacac gtcttccaat gtcagcggct cgaaatagag gcggtcgctg
     3061 gtgtcgaagt cggtggacac ggtttctggg ttgcagttga ccataatcgt ttcaaaaccg
     3121 gattcgcgca gggcgagcgc ggcgtgaacg cagcagtagt caaactcgat gccctgaccg
     3181 atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg acgggattcg
     3241 cattcttctt cgtaagtgga gtaaagatag gcggtttcgg tggcgaactc ggcggcgcag
     3301 gtgtcaacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc gcgcacttct
     3361 ttttctttta cgcccaaaag ctgcgcgatg cgtttgtcgg agaagccttt gcgtttcaga
     3421 cgacgtaggg cggcgaaatc caaatcactc aaaatgccgt ctgaaaccgc tttttcttcc
     3481 ttcatcaagt cttcgatttg cgccaagaac caagggtcga tggcgcagat ttcgtggatt
     3541 tcttccagcg tgaagcccgc gcggaacgcg tctgccacaa acagcatacg ttcggggccg
     3601 gggttcgcca gttcgcggcg gatttccgct ttgtcttcac tgcgcggatt gaagccgcac
     3661 aagcctgttt ccaagccgcg cagggctttt tggaaacttt cttgaatcgt gcggcccatc
     3721 gccatcactt cgcccaccga tttcatctgc gtggtcaggc ggtcgtctgc ggcagggaat
     3781 ttttcaaacg cgaaacgcgg gattttggta accacatagt cgatggaagg ctcgaacgac
     3841 gcgggggttt tgccgccggt gatgtcgttg cgcaactcgt ccagcgtaaa gccgaccgcc
     3901 agcttcgccg ccaccttcgc aatcgggaaa cccgttgctt tggaagccaa cgcggaagaa
     3961 cggctcacgc gcgggttcat ctcaatcaca atcatctcgc cgtttgcagg gttcaccgca
     4021 aactgcacgt tcgagccgcc cgtgtccacg ccgatttcgc gcaataccgc cagcgaagca
     4081 ttacgcataa tttgatattc cttgtccgtc agcgtttgcg ccggcgcaac cgtaatcgag
     4141 tcgcctgtat gcacgcccat cgggtcgaag ttttcaatcg agcagatgat gatgcagttg
     4201 tcgttcttat cgcgcaccac ttccatctcg tactctttcc agccgagaac ggactgctca
     4261 atcaacagct cgtgcgtggg cgacgcatcg aaaccgcgtt cgcaaatcgc caaaaactcg
     4321 tctttattgt aggcaatgcc gccgcccgaa ccgcccatgg tgaaagaagg acgaatcagg
     4381 gtagggaagc cgacctgttc ttgcgccgcc aaagcttcgt tcatcgtgtg gcagacaaaa
     4441 gatttcgggc aggagaggcc gattttctcc atcgcctcct taaagcggcc acggtcttct
     4501 gctttgtcga tggcgtcttc ggtcgcgccg atcagctcga cattgtattt cgccagcacg
     4561 ccgttgcgcg ccaaatccag cgcacagttc agcgcagtct gaccacccat ggtaggcaga
     4621 atcgcgtcag gacgctcttt ggcaataatt ttttccaccg tctgccacat aatcggctcg
     4681 atgtaggtaa catccgccat ttcggggtcg gtcataatcg tggcggggtt ggaattcacc
     4741 aaaatgactt tatagccttc ttcacgcaag gctttgcagg cctgtgcgcc cgaatagtca
     4801 aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat ggattttagg
     4861 tcggtacgtt tgggcatggt gggtctttct tttgaagaat ttggattatt gacgattggt
     4921 aacttctcta attatttctg catctgtttt ttcatcgctt ggattatctg aaaagaaaaa
     4981 actgtaatta ctatcggctt taaatttttc gtatagctct gggcgcaaaa tttttgcttt
     5041 ttcactattt tccacgctcc tgcgattagg cttgccagcc ttatctttcc catttctacg
     5101 gacatcatag caataaatag tgttaggaga attaggttct aaacgagtag ctacaatctt
     5161 tttataaatt atgtctaaat cttttttagt ttgtgttgga atgaaatctg caaaaagatt
     5221 actaaaactc tgaaaaattt ctctgatttt cgcagcacca gcctgaacat taattttaaa
     5281 aaaattaata aaatcagtag cacttttaaa atgaacttca taaaaatcag catacgcaaa
     5341 tattgaattt tcactattca aattaaaaaa ctctaatttt gcttgtgcat aatcatctgg
     5401 tctaggttca tcaggtttat acctagtcaa aataacggcg tattgccttt gattataatt
     5461 aaaaggaaat gcttcaatta ttctattttt gtttctcata tcattaagca aatactttaa
     5521 attattgaaa ttaataggta ttgacatatt taaactcctg cgaatatgtt gcaataaacg
     5581 tagcaatcgt aggttgggtt gaaaacccaa cacaaaccgc ttaattgtct aaaatgttgg
     5641 gtttgctaac ctaacttgta tatcgaaacc cccgtattgt agcaagccgt agcccgtatg
     5701 tagggtatgt gcggtacgta tgcacgcgtt ctccattttc cctgcaaccc caacccaccg
     5761 cgtgcgtgcc ttgcggcaca ctctctgccg atgggtttaa aggtcgtctg aaaaaacgct
     5821 cgcggcgttt gttccatctg ccttgcgata aaggctgcct gaaaaattcc cgctgttgat
     5881 atttcgggca ttctttctca aaccgcctgc aaccgcaaaa tccgctgcaa cctgccccct
     5941 cccccgtggg ggagggttgg ggagagggca ttccccgaat ttcggcaacc tttcccaaca
     6001 actcaaccac cgaaatacaa gccttgtggc ttgttgccct ctctctagct ctctcccacg
     6061 ggagagagga cgggcggttg ttgcgattaa gggttctgca agaaaacagg ttgtgcaggt
     6121 tgctttttgc ttttcagacg acctgaaatc aacggtaaat ccaagtcgct tttcaaaccg
     6181 cctgcaaccg caaaatctgc tgaaacctac tccctccccc gtgggggagg gctggggaga
     6241 gggcattctc cgaatttcgg caacctttcc taacactcta accgccaaga cacaagcctt
     6301 gcggcttgtt gccctctctc taacgctttc ccacagggag agaggacggg gcggctgttg
     6361 gaattaaggg ttctgcagga aaaacaggtt gtgcgggctg ctttttgttt ttcagacggc
     6421 ctttataaat gctcaattca gtcggcttta tcctattatt ttcaataatc caatcaaaat
     6481 cagccatctg ctatcgtgca gcttgctttt ccaattcttg caacacgagc aggatttctg
     6541 tcagcacatc gtttgtctgc tgcaaaatct cgtgattcca aaaccgcagc acggtaaagc
     6601 ccaggctgtt gagatatgcc gtccgcgcgt ggtcgtatac ggcttgttcc gcgtgctgcc
     6661 cgccgtctgc ttcgacaatc agcttgggcg ttacgcacat aaaatcaaca atataattcc
     6721 ccatcggctg ctggcggcgg aatttatagc cgttcagacg acctgcccgc aggtgctgcc
     6781 acaattttgc ttccgccccg ctcatttctt ggcgcatggc tttggcgcgt tggcgcaaga
     6841 tggggttgtc ggtggtcaag agtttctcgt gcgggttcat tttgttgtcg tgctgtttgc
     6901 tgccctctct ctaactctct cccacgggga gagaggacgg ggcggttgtt ggggttaaag
     6961 gggctgtaag gaaaacaagt cgttcaggcc actttttact tttcagacga cctgcaatca
     7021 acggttgtaa atccaagttg cttttcaaac cgcctgtaac cgcaaaactt gctgcaacct
     7081 accccctccc ccgtggggga gggctgggga gagggcattc tccgaatcga ggcaatcttt
     7141 ccctaattcc ctaactgcct caatacaagc ctttcggctt gttgccctct ctctaactct
     7201 ctcccacggg agagaggacg gggcggctgt agtgattaag ggttttgcaa ggaaaacaag
     7261 gaaaacaagt tgtacaggct gtttttttat ttttcaagcg acctgaaatc aacgcttgta
     7321 aatccaagtc gcttttcaaa ctgcctgcaa ccgcaaaatc tgctgcaacc taccccctcc
     7381 cccgtggggg agggctgggg agagggcatt ttccaagttg cggcaatctt tcccaaatcc
     7441 cttagtcttc taaaatacaa gccttacggt tgttgccaaa tgaaacttct tccataccca
     7501 aaacttgcgt gtaaaacgca atggttcggt caatgtcggc aacggtcagt accagatggt
     7561 cgagtgcgct aatcttcatt tcccgctttc tttttcaggc ggcggcagtg ccgtctgaaa
     7621 gccattatgc ccgttttgcc gctttcatat tgccaatgaa tttgtcaaac aaatagccga
     7681 catcttgcgg gccggggctg gcttcggggt ggccttggaa gcagaacaca ggtttgtcgg
     7741 tcagctcgat gccttgcaaa gtgttgtcaa acaaggattt gtgggtaatg cgtgcgttag
     7801 cgggcagggt gtcggcatca acggcaaaac cgtggttttg gctggtgatg acgactttgc
     7861 cgctgtccaa atcttgcaca gggtggttcg caccgtggtg gctgaagcgc attttcaggg
     7921 ttttcgcgcc gatagcgagg ctgatgagct ggtgtcccaa gcagatgccg aaaatcggtt
     7981 tgccgctttc catcagtttt tgcacggctt cgatggcgta ggtgcaaggc tcggggtcgc
     8041 cggggccgtt ggacaggaac acgccgtcgg gattgagtgc caacacgtct tgtgcgctcg
     8101 tttgggcggg gacgacggtc aggcggcagc cgcgcgaggc gagcatacgc aggatgttgg
     8161 ttttcacgcc gaaatcgtag gcgacgacgt ggtaaggctg ttcgtcaggg gtaacgaaac
     8221 ccttgcccaa ttcccattcg ccttccgtcc attcgtaagt ttccgtgcag gaaacttctt
     8281 ttgccaagtc tttgccgacc atactgccga acgcggcgat gagttcttgc gctttttcga
     8341 ttgtggcatc cgcaccggtc agaatcgcgc cgccttgcgc gcctttttcg cgcaacagcg
     8401 tggtcaggcg gcgggtatcg atgtcggcga tggcgacggt ttggttgcgt accaaatagt
     8461 cgtgcaggct ttcggaggcg cggaagttgc tgtgcaagag cggcaggtcg cggataatca
     8521 aaccggcggc gtaaacgctg cggctttctt catcttcggc gttggtaccg gtattgccga
     8581 tgtgtgggta ggtgagggta acgatttgtt tgcagtagga cgggtcggtc aggatttcct
     8641 gatagccagt catcgaagta ttgaacacga cttcgccgga agtcgaacct tcgtaaccga
     8701 ttgatgtgcc gtggaatacg ctgccgtcag cgaggacgag gagggcgggg gtgctcatga
     8761 tgggaatcct gtttttaata aaattcttga caatgccgtc tgaagggact tcggcagggg
     8821 gcatccggtc ggaaaaatcc ggtcaaaaaa aaacacgccg cgcaagatag acgcgtaacg
     8881 tgcttttcgg gcaagtgcct ataccctgaa agcagcgtat tttaagacga aacatacccg
     8941 atttcaagcg caaacgcggc aggcgggctg atatgttcgc gcgaaaacgg aaatccgtgg
     9001 cgtaaaaccg cccgctccgc gcccactatg cccgttacgc atttccccac gtccgcccgg
     9061 cgaaactatg ggaatacccg aatcgtcagg aatacctgaa tcgtcaggaa tacctgaatc
     9121 gtcaggaata cccgaaccat cattcccgcg caggcgggaa tctagacctg tcggcacaga
     9181 aacttatcgg gtataacggt ttcttgagat tccgcattct agattcccgc ctgcgcggga
     9241 atgacggcgc ataagtttct gtgttgattt gagtctatgg gaacggtgta ggattggtgg
     9301 aatcgttgga atcggtgggc tgaagcccac cctataactc accttttacg aatcctccta
     9361 caccctatac aacaccttga aaatccacct tgtcgggaat gcccgaaccg tcagaaatac
     9421 ccgaatcgtc attcccgcgc aggcgggaat ctagacctgt cggcacagaa acttatcggg
     9481 tacaacggtt tcttgagatt ttacgtccta gattcccgcc tg
//
LOCUS       DAXHRI010000072         9423 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXHRI010000072 DAXHRI010000000
VERSION     DAXHRI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.072"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(38..763)
                     /gene="aat"
                     /locus_tag="ACFM75_001256"
     CDS             complement(38..763)
                     /gene="aat"
                     /locus_tag="ACFM75_001256"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC6255800.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACRGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACFM75_001257"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACFM75_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC6255801.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1486..1863
                     /locus_tag="ACFM75_001258"
     CDS             1486..1863
                     /locus_tag="ACFM75_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6255802.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     ASLRPGMTKDQVLLLLGSPILRDAFHTDHWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            1873..2682
                     /gene="dapB"
                     /locus_tag="ACFM75_001259"
     CDS             1873..2682
                     /gene="dapB"
                     /locus_tag="ACFM75_001259"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC6255803.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDTVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2679..2924)
                     /locus_tag="ACFM75_001260"
     CDS             complement(2679..2924)
                     /locus_tag="ACFM75_001260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255804.1"
                     /translation="MPRKQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPFQIRTTH
                     RATLYVSSRVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(2942..3352)
                     /locus_tag="ACFM75_001261"
     CDS             complement(2942..3352)
                     /locus_tag="ACFM75_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255805.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPMPNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGGRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            complement(3354..3599)
                     /locus_tag="ACFM75_001262"
     CDS             complement(3354..3599)
                     /locus_tag="ACFM75_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255806.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(3607..4779)
                     /gene="lpxB"
                     /locus_tag="ACFM75_001263"
     CDS             complement(3607..4779)
                     /gene="lpxB"
                     /locus_tag="ACFM75_001263"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC6255807.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4827..5819)
                     /locus_tag="ACFM75_001264"
     CDS             complement(4827..5819)
                     /locus_tag="ACFM75_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6255808.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            6314..9073
                     /locus_tag="ACFM75_001265"
     CDS             6314..9073
                     /locus_tag="ACFM75_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC6255809.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETAAVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIAEPVA
                     ERDVPTASPAVPNNDAPVYDAAEKIRQVVAAILPEGAAPEAAAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPTEEMIQV
                     ETRQG"
BASE COUNT         2366 a         3006 c         2216 g         1835 t
ORIGIN      
        1 ccaccatatt tgaacctgac cggagcataa gccgccatca aagccccctg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatccgcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgtg tcctgctggc
      181 agtctatcag ttccacaccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgtaat gcgaacatcg attcgccgta aaacaccctg ccgatttgca
      301 cgccgtaaaa gccgcccgcc aacctcgttt taccgttttc atcgggataa tggcactcga
      361 aagaatgcgc gtaccccatt tcgtgtagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagttcc gtcctgattc gggcgcgccg ctgccgcaca atgcgcgacc acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttgcgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaatcaccg cacgcgggcc gaccgcatac caaaaaaacc
      601 aaccgtcccg agaaaaccac ggaaacacgc cgttccgata cgcctcaagc agccgccccg
      661 cgtccaaatc cctgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcctcagcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tgtggtcatg gtggtcgcct ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatgat ggcgttgcag aatacccgcc tgctcaaact
     1081 gggtcagcac acggtaaatc gtcgccacac cgatttccac accctcttcc aacaaaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcatgcgt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgtgcaata ttgttgaatt
     1261 tttccataat attcaatacc cctgtaaaac aatagacgtt ataatacgca atttcggcct
     1321 gtttgcccac tatcgcacca tagcagtttg caatagcaaa accgcaacgg cggccgatgc
     1381 gcggacaggg cggataccgc ttaatcgtat gattatcgtt cgcttttata aaatattcaa
     1441 gcagtgttac actacccatc ccgatttgca cagaaaggca tttccgtgaa caaaaccctc
     1501 atcctcgccc tttccgccct cctcggcctt gccgcgtgca gcgtcgaacg cgtttcactg
     1561 ttcccctcgt acaaactcaa aatcatacag ggcaacgaac tcgaacctcg cgccgtcgcc
     1621 tccctccgcc ccggtatgac caaagaccaa gtcctgctcc tgctcggcag ccccatactg
     1681 cgcgacgcat tccataccga ccactgggac tataccttca acacctcccg caacggcatc
     1741 atcaaagacc gaagcaatct gaccgtctat tttgaaaacg gcgtgctcgt ccgcaccgaa
     1801 ggcaacgccc tgcaaaatgc cgccgaagcc ctccgcgtaa aacaaaacgc agacaaacaa
     1861 taaggaacac acatgacacc gctcaaaatc gccatcgccg gcgcaaacgg ccgtatgggg
     1921 cgcgtcctcg tcgaagccgt caacaaccat cccgacaccg tcctttccgg tgcgcttgaa
     1981 cactcaggct cagaagccct cgggctggac gcaggctacg ccgtcggact caaaaccggc
     2041 atcgccattt cagacgacgt tgacaccgtt ctcgcacaaa gcgacgtact catcgacttc
     2101 acccgccccg agcccaccct caaacacctg caaaaatgcg ttgaaaaaca agtcaacatc
     2161 atcatcggca caacaggctt cgacgatgcg ggcaaagccg ccatccgcgc tgccgccgaa
     2221 aaaacaggca tcgttttcgc cgccaacttc agcgtcggcg tcaacctcac cttccacatc
     2281 ctcgacaccg tcgcacgcgt attaaacgaa ggctacgaca tcgaaatcat cgaaggccac
     2341 caccgccaca aagttgatgc ccccagcggc accgcgttac gcatgggcga agtcatcgcc
     2401 ggcgcgctcg gccgcgacct caaacaatgc gccgtttacg gccgcgaagg ccacaccggt
     2461 ccgcgcgatc cgtccaccat cggctttgcc accgtccgcg caggcgacat cgtcggcgac
     2521 cacaccgccc tcttcgccac cgacggcgag cgcgtggaaa tcacccacaa agccggcagc
     2581 cgcatgacct ttgccgccgg cgccgtccgc gccgcagttt gggtaaacgg caaaacgggt
     2641 ttgtacgata tgcaggacgt actcgggctg aacaaccgtt aacccccata caaaatgccg
     2701 tctgaaaaga tattgttcag acggcatttt gccgacaggc tccgtatcgg catatgtttc
     2761 aacacacggg acgatacata aagcgtcgcc ctatgtgtcg tcctgatttg gaagggatta
     2821 cgcccctccc aaataaagtc tgattctacc gccccgaagg acagatgtcc gagtggcggg
     2881 gtttcaaccg aaaaggaaat acgataaagt tgcttgcgcg gcatgacaag ttacggctcg
     2941 tttaattaaa actctcctga tctaaaaatt ctaactctat ttcccggcaa actatatcta
     3001 tactaaaacc ggtcggaaga tcatacctac cgcctatcac ttccagcgca cctccataat
     3061 ccaagatttc atcatcctgc tttaattctc caagttccaa tacatttttg atatttttat
     3121 cttggttctt cgtggcgtat ccatagataa atttcagata gctgaactgc ttgaatttaa
     3181 tttttaccca ttctgaataa ttgggcatag ggatctcttg aggaaagtcc ggataatcca
     3241 cattctggac gatgatatgg aattcatcgt ttttcaaagt atagtttaat agagtgccct
     3301 catatatata cagaaattga taatcttttt ttggaatggt ataagttgtc atattatttt
     3361 accttttcaa aaacaatgag tttaagattt tttgagtcca agttggttct actactctta
     3421 tgaaatttca gttgtttaaa ctcttccttt aacagaccat tatattttaa ccttaatccc
     3481 actcgagcca gttctgcttc atttggaatt cctcttaaat acttattgtt tgatgtgcca
     3541 ttaatcacaa tttctccccc tttttttgta atacgtgctg cttcgggaaa ataatccatt
     3601 aaccgctcaa catcccgcct cttccaaaac cgcgcgcgcg gccaaatccg ccgtatcttt
     3661 tttcaacagc aggtgcaacg ccctgaaatc ctgttgcagc gcggcaacct tatcggggtg
     3721 ttcgtaccag tccgccaacg ccgccgccag tttttccggt tttgcttcag attgcaataa
     3781 ttccggcaca gcctccttac ccaacaggat attcggcagg ccgacatgcg gcactttgat
     3841 tttgcgtttc acataagcat aagtcagcgg cgaaatcttg tagctgatga ccatcggacg
     3901 cttgcacaac gccacctcca aagtcgccgt accgctcgtt accagcaccg catccgccgc
     3961 cctgcacact gtttcagact gtctgtcgat taccgtcagc ggcaatccgg caaactccgg
     4021 ccgctgcaaa acttccgcca aacgccgctt cgtcgcctcc gttgcggcag gcagcaggaa
     4081 gcatgcggca ggatagcgtt ccaacaacaa taatgccgtc tgaaaaaaca ccggcgccat
     4141 atagtcgatt tcgctgacgc ggctgccggg cagcagggcg aatacgggga tgccggcatc
     4201 cacgcccaaa gtttgccgcg ccgtttcacg gtcgtcttcc aagggcataa gctgcgccat
     4261 cggatgaccg acaaactccg cacgtccgcc cgcatcgaga taaagctgcg gctccatcgg
     4321 gaacaggcac aacacgcggt tgacctgatg cacgattttg cccacacgtt cccgccgcca
     4381 cgcccacacc gacgggctga cataatgcac ggtcggaatc cccgaccgtt tcagcttttc
     4441 cgccacaccc aaattaaaat cgggcgcatc gataccgaca aagacatcag gtttcaacga
     4501 cagcaaatcc cgtaccagcc ccctgcgtat ccgtaaaatt tccggcagcc gcctgaccac
     4561 ttcgacaaag ccgcgcaccg ccagccgctc ctgatcataa aggctctcga aaccttccgc
     4621 cttcatcagt tcgccgccga taccgacaaa acgcgcctgc ggacaacgct tgcggatggc
     4681 gcgtatcagg tgcgccccca acaggtcgcc cgacgcttcg ccgacactga cggcaatcaa
     4741 agggcttttt ttatcaacca tattcgtctg tccccacata ctttcacgtc ccgatgccgt
     4801 ctgaaggctt cagacggcat cggggttcac attttcgtcc cgttttccaa catcaccgca
     4861 aactgtgcca agtccggcgg cagctccgcc ttcaacacca gcggctcgcc cgtgagcgga
     4921 tggttcaagt gcagctcgga cgcgtgcaaa aacatccgct tcaaacccaa cttctgcaaa
     4981 cgacggttcg cctgataatc gccgtagcgt tcgtcgcccg caatcggaca gccttgagat
     5041 tgcaggtgga cgcggatttg gtgcgtgcgc cccgttttca acgtcgcccg caccaaagtc
     5101 aggtgcgaca gcccgacacc gtgcaaaatg ccgtctgaaa aacggcttaa cacacggaac
     5161 accgtatgcg ccgactgccc gtccgcactg acgcgcacca tcttttcgcc ttgtgcgccg
     5221 gtatatttga acaggggcag tttgacatgg aaattgtcgt ccggcagttt gcccaccccc
     5281 agcgcaaggt agattttttt ggggtggtcg ttacggatgg cttcgtgaag tttgacgagc
     5341 gcgctgcgtt tcttcgccac catcaacaag ccgctcgtat ccttgtccaa acgatgaacc
     5401 aactccaaat acttcgcctc cggacgggcg cggcgcaact gttcgataac gccgaaactc
     5461 acgccgctgc cgccgtggac ggccacgcca gaaggcttat cgatcaccaa aagcgcatcg
     5521 tcttcgtaaa caacgtcaaa cgcacgcgcc ggtacggcgg cacgcctttc agacggcatt
     5581 tccttctccg ccacgcgcac aggcggaatc cgcaccgtat ccccctccgc aatacggctg
     5641 tcgggtttgc agcgtttttt gttcagccgc acctcgccgg cgcggataat gcggtggata
     5701 tggctcttgg gaaccccctt gaggattttt atcagatagt tatcaaggcg ttgacccgcc
     5761 tcatgttcgg caacccctat caagctgacc gaatctttgc ttatttcgtg cgttttcatc
     5821 tataatccga acatccgttt cagcaaaaag cgcgcccgcc gcgcccccct gaaacggttt
     5881 actttaaaac ggaattttat attaaaaacg cactccgcaa agcattttcc ctgcgcctgt
     5941 tccaagccgg caggcgcaga acgtagtcaa gtttgaattg attttgccgt ttcggagcgg
     6001 aagtaagacg gcagccgggt ccaagcccca aacgcaaacc gcccgaaaac acaggcatca
     6061 cgataacaag aagaagccgg cctcccattt ttccgaccat accgctcgga acgcggcaga
     6121 cagaccgccg catcgatctt tattgccttc tttatccgac ccttccgcac acggttcctc
     6181 ggaacgtgca tccctgcgga ttttcaactc aaaaactgat taccggtttt atccgaagac
     6241 atcggaaaac ccagccttac ggaatgccga accggaccgg cgcgcccgaa tgccgcccgt
     6301 ccacgaggtg atcatgaaaa gaatgttatt caacgcaacg caggccgaag agctacgcgt
     6361 tgccatcgtc gacggacaaa acctgctgga tttggacatc gaaacgctgg gcaaagaaca
     6421 gcgcaaaggc aatatctaca aaggcatcat tacccgcatc gagccgtcgc tggaagcgtg
     6481 tttcgtcgat tacggaaccg accgccacgg ctttttgccg tttaaagaag tctcccgctc
     6541 atacttccaa gactacgaag gcggacgcgc gcgcatccaa gacgtgctca aagaaggcat
     6601 ggaagtcatc gtccaagtcg aaaaagacga gcgcggcaac aaaggcgcgg cactgaccac
     6661 cttcatcagc ctcgccggac gctatctggt attgatgccg aacaacccgc gcggcggcgg
     6721 cgtatcccgc cgtatcgaag gcgaagagcg tcaagaactc aaagccgcca tggcggaact
     6781 cgacattcca aacggcatga gcattattgc ccgcaccgca ggcatcggcc gcagcgcgga
     6841 agagttggaa tgggatttga actacctcaa acaactctgg caggcgattg aagaagcagg
     6901 aaaggcgcat catgacccct acctgctctt tatggaaagc tcgctgctca tccgcgccat
     6961 ccgcgactat ttccgccccg acatcggcga gattttggtg gacaatcaag aagtttacga
     7021 ccaagttgcc gagttcatga gctacgtcat gccgggcaat ataggccgtc tgaaactcta
     7081 cgaagaccac acgccgctgt tttcccgctt ccaaatcgaa catcaaatcg aaagcgcgtt
     7141 ttcgcgcagc gtcagcctgc cttccggcgg cgcgatcgtg atcgaccaca ccgaagccct
     7201 cgtttccatc gacgtgaact ccgcacgcgc cacacgcggc gcggacatcg aagacaccgc
     7261 gttcaaaacc aatatggaag ccgccgaaga agtcgcccga caaatgcgcc tgcgcgactt
     7321 gggcggtttg gtcgtcatcg acttcatcga tatggaaaac cccaaacacc agcgcgatgt
     7381 ggaaaacgtc ctgcgcgacg cgctcaaaaa agaccgcgcc cgcgtgcaga tgggcaaact
     7441 gtcccgtttc ggacttttag aattgagccg ccaacgtttg aaaccggctt tgggcgaaag
     7501 cagccatgtt gcgtgtccgc gctgtgccgg caccggcgtg attcggggca tcgaatccac
     7561 cgccctgcac gttttgcgca tcattcaaga agaagcaatg aaagacaaca ccggcgaagt
     7621 gcgcgcacaa gtgcccgtcg atgtcgccac cttcctgctg aacgaaaaac gcgccgagct
     7681 gtttgcgatg gaagagcgtt tggatgtcaa cgtcgtcctg attccgaaca tccacctcga
     7741 aaatccgcac tacgaaatca accgcatccg caccgacgac gtagaagaag acggcgaacc
     7801 gagctacaag cgcgttgccg agccagaaga agacgaatcc gccaaaccgt tcggcggcga
     7861 aaaagccaaa gccgcccgtc ccgaacccgc cgtcaaaggc gtgcgccaca ccagccccgc
     7921 cccgactgcc gcccccgaga aaaaaacctc ctggtgggac agcttcaaag cttggctcaa
     7981 acgcattttc ggcggcagcg aaacccaagc cgcgcccgct gccgaaacct ccgaaaaacg
     8041 cagcacggca aaccgcagcg gcagccgcgc caacaaccgc cgccaaaacc cgcgccgcag
     8101 caaacgcgaa ggcagcaaag tggaagtccg cgaagtggca ggcaaaactg ccggacagga
     8161 agcgcgtgcc gacaaagccg aaacgcgcaa caacggcaac cgccgccgca acgaacgcgg
     8221 cgaccgtgcc gccgaacgcg ccaacgaagc ggaaatccaa gaccgcaaca tacagcctgc
     8281 cgcaaccgtt gcagatgccg caccgtccga aaccgaagtg caaaccggaa aacgccgccg
     8341 caacggcagc cgcagcgaac gcggccaaac cgcgccggaa accgccgccg ttgccgaaac
     8401 aaccgttcag acagcggaaa acacgccgtc cgaaccgcat accgcagaag acaaaggcag
     8461 caagcccaaa tccgaacgca accgccgcga acgcgacagc cgagatgcca aagaacgccg
     8521 cgagcgcaac aatcagcgcg accgccgtca aaacggcaaa aaacgcaata ttccgtctgc
     8581 cgccaaaatc gagcagtacc tgaatattca cgacaccgcc gacaaagtcc gttccgccgc
     8641 cgcacacgtt ttcggcgaaa ccgacgcaaa cgcgcccatc accgtcagca ttgccgaacc
     8701 ggttgcagaa agggacgttc cgacagcatc tcccgccgtt ccaaacaacg acgcaccggt
     8761 ttatgatgcg gcagaaaaaa tccgtcaggt cgttgccgcc atcctgcccg aaggcgcggc
     8821 accggaagcc gcggcacagg aaatgccgtc tgaaaccgca acctttacgg ctgcggcgga
     8881 acaggcacgg gaaaccgcac aaaccggcgg actcgtcctg atcgaaaccg accctgccgc
     8941 attgaaggca tgggcggcac aacccgaagt ccaagccgga cgcggtttgc gccgttccga
     9001 acagcccaag ccgtctgaag tcgcaaccgt cccaaccgaa gaaatgatcc aagtcgaaac
     9061 ccggcaaggc tgaaccgacg gcggcaaaaa gaggttctgt tccgcagaac ctctttttta
     9121 tatagattcg gatacctgca atgccatctg aaacttcgcc attcccgtga ttaccgaaac
     9181 attccgccat tcccatgatt cccgcaacat tccgtcattc ccgtgaaaac gggaatctag
     9241 aacctcaaac tttcggataa tctttgaata ttgccgtcgc ccgaaggcct ggattcccgc
     9301 ctgcgcggga atgacggcgg agggtggacg atgccgtctg aaacttcgcc attcccgtca
     9361 ttcccgcaac attccgtcat tcccgtgaaa acgggaatct agaacctcta aactttcaga
     9421 taa
//
LOCUS       DAXHRI010000073         9030 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXHRI010000073 DAXHRI010000000
VERSION     DAXHRI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.073"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            198..1040
                     /locus_tag="ACFM75_001266"
     CDS             198..1040
                     /locus_tag="ACFM75_001266"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC6255810.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1069..1533
                     /locus_tag="ACFM75_001267"
     CDS             1069..1533
                     /locus_tag="ACFM75_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC6255811.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGLRD
                     IPSAELADMVLKELLEHNTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1565..2674
                     /gene="ribD"
                     /locus_tag="ACFM75_001268"
     CDS             1565..2674
                     /gene="ribD"
                     /locus_tag="ACFM75_001268"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC6255812.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETAGGK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNDNAF"
     gene            2869..4287
                     /locus_tag="ACFM75_001269"
     CDS             2869..4287
                     /locus_tag="ACFM75_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC6255813.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDILFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLLTV
                     GLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPI
                     ALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRA
                     IEANAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPL
                     FCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFWL
                     FFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAVYLAGC
                     ILRHRKDLHKLFHYLKKQGFPL"
     gene            4284..5356
                     /locus_tag="ACFM75_001270"
                     /pseudo
     CDS             4284..5356
                     /locus_tag="ACFM75_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            5376..6544
                     /locus_tag="ACFM75_001271"
                     /pseudo
     CDS             5376..6544
                     /locus_tag="ACFM75_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            6537..7064
                     /locus_tag="ACFM75_001272"
                     /pseudo
     CDS             6537..7064
                     /locus_tag="ACFM75_001272"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            7113..8114
                     /locus_tag="ACFM75_001273"
     CDS             7113..8114
                     /locus_tag="ACFM75_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFC6255814.1"
                     /translation="MPYFTGNQTNEKNNILILSAGRRVELVQDFQTEAARFSDGISVF
                     ATDLNPRMSSACHVSDGSFEVPPISADSYIDSIFNLAVRENIGLIIPTIDTELQKLAD
                     ERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPY
                     DGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAI
                     PRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCITAQFFYNEETGEFYGVEI
                     NPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            8101..8787
                     /locus_tag="ACFM75_001274"
     CDS             8101..8787
                     /locus_tag="ACFM75_001274"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6255815.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
     gene            8835..9029
                     /locus_tag="ACFM75_001275"
     CDS             8835..9029
                     /locus_tag="ACFM75_001275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255816.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHDRR
                     GQSGKAAAAQKPQDRDGISD"
BASE COUNT         2036 a         2840 c         2232 g         1922 t
ORIGIN      
        1 caaaagttac ggaacgaaaa acaaccaaaa ccggacaagt cggattcccg cctgcgcggg
       61 aatgacggaa tcttaagttt ccgtctttgt tttctgtttt cgagggaata atggggaaca
      121 agccgtattt cagacggcat tttcagttcg gggtataatc cgaatacttg cgaccatctg
      181 aatcattggg acaaaccatg tgtcaactgc tgggcatgaa ctgcaatacg ccgaccgata
      241 ttatgttttc ctttgaaggc ttccgccgca ggggcggcat taccgaccac catgccgacg
      301 gtttcggcat cggctttttc gaaggcaaag gcgtgcgcct gttccatgac gacaaaccca
      361 gtgccaattc gcccgtcgcc gacctcgtgc gcgcctacca gattaaatcg gaaaacgtcg
      421 tcgcccatat ccgcaaagca tcacaaggac aaacctcgct ggcgaacacc catcccttta
      481 tgcgcgaaat gtggggcggc tactggctgt ttgcccacaa cggacatttg attgattttt
      541 ttcccgaaca gggcgaattt ttccaccccg tcggcacaac cgattccgaa cgcgcgttct
      601 gccacatcct caaccgcctg cgcacccgct ttgccgcccg tcccgacgac gacacgctgt
      661 ttgacgcgat tgcggggctg acgcacgaaa tccgcaagtt cgggctgttt aactttatgc
      721 tttcagacgg cattgccctg tttgcccacg ccagcacact gctgcactac atcgtccgcc
      781 aagccccgtt cggcaaggcg cgcctgctcg acgacgacgt gatggtcgat tttgccgaag
      841 taaccacgcc ctccgaccgc gtcgccgtta tcgccaccct gccgctgacc cgcgacgaat
      901 catggtccca acttgccgtg gacgaactgg tcatgttccg cgaaggcaac atcgtccgac
      961 acgaccgtcc cgaaaacccc gtctatatga gtgccgaaga aggtctggaa atcgcccgcg
     1021 ccgccggcgt atcggtttga cgttcagacg gcatcaggaa gaaacgccat gaaatgcccg
     1081 ttttgcgccc accccgacac ccgcgtcgcc gattcgcgcc tgatggaaga acgcaacgcc
     1141 gtgcgccgcc gccgccactg ccccagctgc ggcaaacgct tcggcacgct cgaaaccgcc
     1201 gaactcaaaa tgcccgccgt catcggtccg gacaaaaaac gttcgccctt taatgcacaa
     1261 cgcctccgca acgacctgac cgccgccgcc cgaaaatccg ccctgacacc cgaacagatc
     1321 gacgaaaccg tccgcctgac ggaacacagg ctctacactt cgggtcttcg cgacatcccc
     1381 tctgccgaac ttgccgacat ggtgctaaaa gagctgcttg agcacaacac ggaagccgcc
     1441 gtccgtttcg ccgcactgca caaacgcttc gacaatccgg cagactttgc ctcgtggctg
     1501 gcgcaagccg tcaaaacagg cggcaaagcc tgattccccc aacccatact gatacggtat
     1561 ccccatgttt tcggacacag atatatccat gatggaaaac gccctccgac ttgccgcttt
     1621 ggggcgtttt tccacttcgc ccaatccgcg cgtcggctgc gttatcgcac acggcagcca
     1681 aattgtcggg cagggcttcc acgtcaaagc gggcgaaccc catgccgaag tccacgccct
     1741 gcgtcaggcg ggcgaaatgg cacaaggcgc gaccgccttt gttaccctcg aaccgtgcag
     1801 ccattacgga cgcacaccgc cctgcgccga agctctgctc cgttccggcg taacacgcgt
     1861 cgtcgccgcc atgcgcgacc ccaacccgct ggttgcaggc aaagggcttg ccctgctcga
     1921 agcagcaggc atcaagacgg aatgcggttt actcgaacat caggcaaggg aactcaaccg
     1981 aggcttcctg tcgcgcatcg aacgccgccg ccccttcgtc cgcctcaaat gcgccgtttc
     2041 gctggacggc aaaaccgccc tttcagacgg cagcagcttt tggattaccg gcgaagacgc
     2101 gcgtgccgac gtacaggttt tgcgtgccga aagctgcgcg gtgctgaccg gcatcggcac
     2161 ggtgttggcg gacaatcccc ggctcaacgt ccgcgctttt ccaactttgc gccaacccgc
     2221 acgcatcgtg ttagacagcc gcctgcgcct gcccttggac agccaccttc tgaccgacgg
     2281 acaatctcca acctacatcg ccaccttgga acgcgatgaa gataaactcc gcccctatcg
     2341 ggaacacgca cacatccgca tcctgatgcc gtctgaaacg gcaggcggca aaatcgacct
     2401 gaaccacctg atgcgcctcc ttgccgacga aggtttcgga gaaatcatgg tcgaagcagg
     2461 ctccgaactc acatccgcat ttctggcaga agatctggcg gacgaaatcg tgctgtaccg
     2521 ttcgcccaaa atcctcggca gcggcaaaga cctgctttcc ctgcccgaaa accgcgccgc
     2581 cctttccgca ccgcccttgt ggacacccgt ttcaagcgaa atcctcggac acgacatcaa
     2641 aaccgtgttc cggaaaaacg ataacgcctt ttaaagggtt tgcgccgttt cactatataa
     2701 taacgccgat aaaaaacggc cccgttcaga ccgccgcccg cccggaaaag cgcaacccgg
     2761 actgccgcac cccgccggca gccgcaacgg actgaaagcc gtcaaattcc gatcaaaaaa
     2821 ggcttcagac ggcaccggcg gcatcccgcc tccgcccgga catcaaaaat ggacacaaaa
     2881 gaaatcctcg gctacgcggc aggctcgatc ggcagcgcgg ttttagccgt catcatcctg
     2941 ccgctgctgt cgtggtattt ccctgccgac gacatcgggc gcatcgtgct gatgcagacg
     3001 gcggcgggac tgactgtgtc ggtattgtgc ctcgggctgg atcaggcata cgtccgcgaa
     3061 tactatgccg ccgccgacaa agacattttg ttcaaaaccc tgttcctgcc gccgctgctg
     3121 tctgccgccg cgatagccgc cctgctgctt tcccgcccgt ccctgccgtc tgaaatcctg
     3181 ttttcgctcg acgatgccgc cgccggcatc gggctggtgc tgtttgaact gagcttcctg
     3241 cccatccgct ttctcttact ggttttgcgt atggaaggac gcgcccttgc cttttcgtcc
     3301 gcgcaactcg tgcccaagct cgccatcctg ctgctgctgc tgacggtcgg gctgctgcac
     3361 tttccggcga acaccgccgt cctgaccgcc gtttacgcgc tggcaaacct tgccgccgcc
     3421 gcctttttgc tgtttcaaaa ccgatgccgt ctgaaggccg tccggcgcgc accgttttca
     3481 tccgccgtcc tgcatcgcgg cctgcgctac ggcataccga tcgcactaag cagcatcgcc
     3541 tattgggggc tggcatccgc cgaccgtttg ttcctgaaaa aatatgccgg cctagaacag
     3601 ctcggcgttt attcgatggg tatttcgttc ggcggagcgg cattattgtt ccaaagcatc
     3661 ttttcaacgg tctggacacc gtatattttc cgcgcaatcg aagcaaacgc cccgcccgcc
     3721 cgcctctcgg caacggcaga atccgccgcc gccctgcttg cctccgccct ctgcctgacc
     3781 ggcattttct cgcccctcgc ctccctcctg ctgccggaaa actacgccgc cgtccggttt
     3841 atcgtcgtat cgtgtatgct gccgccgctg ttttgcacgc tggtagaaat cagcggcatc
     3901 ggtttgaacg tcgtccgaaa aacacgcccg atcgcgctcg ccaccttggg cgcgctggcg
     3961 gcaaacctgc tgctgctggg gcttgccgta ccgtccggcg gcgcgcgcgg cgcggcggtt
     4021 gcctgtgccg cctcattttg gctgtttttt gttttcaaga ccgaaagctc ctgccgcctg
     4081 tggcagccgc tcaaacgcct gccgctttat atgcacacat tgttctgcct ggcctcctcg
     4141 gcggcctaca cctgcttcgg cacgccggca aactaccccc tgtttgccgg cgtatgggcg
     4201 gtatatctgg caggctgcat cctgcgccac cggaaagatt tgcacaaact gtttcattat
     4261 ttgaaaaaac aaggatttcc attatgaaaa tcatcctgac cacttctatg tcgggcttgg
     4321 gcggcacgga aaccgccacc gtccgcctcg ggcggctgct caaacggcgc gggcacgaca
     4381 tcatccttgc ttcttcggac gggccttttg tcggagaggc gcaagcatcg ggcatccgtt
     4441 ggcagccggt tgatttttac tggggcgggc ttgccggcta cctcaaaagc acctttgcct
     4501 acgcccggat gctgcgccgc gaacaacccg acatcatcga ctgccaaatg gcgcgtgttg
     4561 ttccagcctg cgcccttgcc gccaaaatcg tttcgccgaa aaccaaaatc atctaccact
     4621 cgcacggttt ggacgcggca acctacccca aaatcgccaa actcttcgac aagctgggcg
     4681 tgtacatcat cggcaactgc aaacacgagc gcgaaaaact catccgccac ggcttccccg
     4741 caggccggat tgcctacgcc tacaacgcac tccccccccc ccggaatttc atttccggaa
     4801 aaccgaaaaa gaatgcgccg tactcggcac gctttcccgt ttggacaccg tccgcgccgt
     4861 gcatctgatg ttggatattt tgaagaaaat ggttgactgc ggtatgcccg tccgcctcaa
     4921 catagcgggc ataggagaag aaatggacaa tttgaaagcg caggcggaac gtttgggcat
     4981 agatgacaaa gttaccttcc tcggcggcat acgcgacttg accggctact tcaaagaaat
     5041 cgatattttg gtgaacacgc cgcattgcgt gggcgaccac ggcgcaggtg tcggcaacaa
     5101 catcttggaa gccggccttt acgacacgcc cgtcgttacc tacgatatgg cgggcatttc
     5161 cgaaatggtc attaccggcc gaaccggcta ctgcatcccc ttcggcgacg aagaagcctt
     5221 tatcgccgcc gtagacacgc tcgtcagaca accggagttg cgcgaagcaa tgggcaaaag
     5281 gctgcacgca cacgtcgccg cactctgttc cgacgacgaa atctaccgta ccaccatggc
     5341 ggcgtatgcc atgtaatgcc gtctgaaagc cgccgatgaa catcaccata gtcgccccct
     5401 actgctcgct gccgtccgaa ccgtatttca accgcttttg gtatctggca gaacgattgt
     5461 cgcaatcgca cgacgtgttg ctgattacca gccgtttccg ccactacgac aaatccttca
     5521 gacggcccga agatgccgag gccgcctcgc aagaccgtct gaaagtcatg ctgttggaag
     5581 aaagcggcta cggcaaaaac gtgtctttag aacgtgttgc cagccattac cgcttcgtca
     5641 aaaattttga gcaatggctg aaaaactgcc ggccgggcga acaagacatc gtctattccg
     5701 cctatccgct gattgccacc aacctgctgt tgggcaaaca caaagcgcgt ttgggctaca
     5761 aactgattgt cgatgtgcag gacgtatggc cggagtcttt ctcctcggtc gtaccgtttt
     5821 tgaaaaaagt accgcacaag ctgctgccct ttgcttcacg cgccaaccgt gcctaccgct
     5881 gcgccgacgc gctggttgcc gtatcgcaga cttatctcga ccgcgccaaa gaagccaatc
     5941 cgaacgtacc cggcgaagcc gtctatatcg gtacggattt tgccgccatc gccccccccc
     6001 cccgcgattc cgctccaaaa ccgtccgcct tttctacttg ggcacgctca gttacagcta
     6061 cgacgtggaa accgtgtgca aaggtgttcg gaaactttta gacgacggcg aaaacgtcga
     6121 attgcacatt atgggcggag gacccgattt ggagaaactc aaacaatatg aaaatcgcgc
     6181 catcaagttt tacggttatt taccctattc tgaaatgatg tcgattgcca aaggctgcga
     6241 cattgccgtc aacgccatcc atagccacgc catgcagtcg gttaccaaca aactctccga
     6301 ctatatggct ttgcaaaaac cgattctgaa cagtcagaac aatgccgaag tcctcgacct
     6361 catgaatctg ctgccgcacg aaaactaccg ttccggcgat gtggacggtt tcgttcaagc
     6421 cgccaaagat attttgaagc gcaaaagcga tcctgttcag tccgacgaaa tcgtccgccg
     6481 cttcaaacgc gatgtggcgt atcagaaaat cgtcaacctg attgaaagat tagctcatga
     6541 ataaattttt caaacgcctg ttcgacatca tcgcctccgc atcggggctg attgtcctct
     6601 cacccgtttt cctgattttg gcgtacctca tccgcaagaa tctaggttcg cccgtcttct
     6661 tcattcaaga acgccccgga aaggacggaa aaccttttaa aatggtcaaa ttccgttcca
     6721 tgcgcgacgc gcttgattca gacggcattc cgctgcccga cagcgagcgg ctcacaccct
     6781 tcggcaaaaa actgcgtgcc gctagtttgg acgaactgcc tgaattatgg aatgtcttaa
     6841 aaggcgacat gagcctggtc ggcccgcgcc cgcttttgac gggctacctg ccgctttata
     6901 acgacttcca aaaccgccgc cacgaaatga aaccgggcat taccggctgg gcgcaggtca
     6961 acgggcgcaa cgcgctttcg tgggacgaaa agttcgcctg cgatgtttgg tacaccgaca
     7021 acttcagctt ttggctggat atgaaaatcc tgtttttgac agtcaaaaaa gtcttgatca
     7081 aagaaggcat ttcggcggaa ggggaagcca ctatgcctta tttcactgga aatcaaacta
     7141 atgaaaaaaa taatatactg attctgtcgg caggtcgccg tgtcgaattg gtgcaggact
     7201 tccaaaccga agccgcacgt ttttcagacg gcatcagcgt ttttgccact gacctcaacc
     7261 cccgcatgtc ttctgcctgc catgtttcgg acggttcatt tgaagtgccg ccgattagtg
     7321 ccgattcata tatcgacagc attttcaatc tggcggtacg cgaaaatatc ggtttaatca
     7381 tcccaaccat agacaccgaa ctgcaaaagc tggcagacga gcgcgaacgc tttgaagcgg
     7441 caagtgtcca catcgtcgtt tccgattcgg acttcatcgc acaatgccgc gacaaacgga
     7501 aaaccgccac cttgtttgcc cgctacggca tacgctcacc cgaaatttac gaccgcaaga
     7561 agttggtttt cccttgcttc gccaagcctt atgacggcag ccgcgccatc ggtgcaaaac
     7621 ggattgacac acctgccgac ctgacaccgg aagtcttggc agacaccaaa ttaatgttct
     7681 gtcagtttat cgacatcgaa aataaatttt ccgagtttac cgtcgatatg tattacgacc
     7741 gtcaatgccg tctgaaatgc gccatcccgc gaaaacgctt ggaagtgcgc tccggcgaaa
     7801 tcagcaaagg cgttacctgc aaaaacagcc tctaccaaac gctgctggaa aaaatgtcgg
     7861 tattgcaggg cgcgcgcggc tgtatcacgg cgcagttctt ctacaacgaa gaaaccggcg
     7921 aattctacgg cgtggaaatc aacccccgct tcggcggtgg cttcccgctg acgtatgccg
     7981 caggcggcaa ttatccgagc cggttgatgc gcgaatacct cggcgacgaa gaaatcccgt
     8041 tttcagacga ctgggaaaac aacctcatta tgttgcgcta cgatgccaag gtcttggttc
     8101 atgaaaacga ttaatcctga aaccaccgtc atcgtcttcg acttggacga taccctgtat
     8161 tcggaatacg aatacaagct ctccggtatc cgttcggtca tcgataccgt tgccgcactg
     8221 tatcccgatt ggaattcaga caacctatgg cgcaacatcg accccgacgg caaagattgg
     8281 ctggacaaat tgtgccgcca ctgtggtttt aatgaatcgg aaaaacaggt tcttttgtgg
     8341 caataccggc tgcaccgccc gacgctgact ccgtatgctc cgccggattt cctgtccgaa
     8401 ctgaccgcgc ccttcgccgc acgcgcgctg attaccgacg gcagaagcct gacccaacgc
     8461 ctgaagctgg aagccttggg tctgtcttcc ctgtttgacg acatcctcat ctctgaagcc
     8521 tgctcttcgg aaaagcccga cggcaaacgc ttccgccatc ttcaagacaa atatgccgac
     8581 aaggcaggct gcttcatcta tatcggcgac aacatttcca aagactttat cgcgccgaac
     8641 actttgggct ggataaccat aggattgctt ccctccggac agaacatcca ccgccaccag
     8701 cctgaaagtt ttaacacaga atatcatccc gacttttggg ttaactccct tcaagattta
     8761 gttcctataa ttgattcaaa tacttaacca ttacataatc aaacatgcac tcacaatatt
     8821 aaaaggacta tttcatgaca aaatattatt catggggcgt aaacggcttt ccgccctacg
     8881 ttttcgacac catttggaaa cgatggaagt tgaatgggcg cgggggcggt tatcgtgtgc
     8941 gacatcccgg acggcatgac cgtcgcgggc aatccggcaa agccgctgcc gcgcaaaaac
     9001 cccaagaccg ggacggcata agcgattaaa
//
LOCUS       DAXHRI010000074         8803 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXHRI010000074 DAXHRI010000000
VERSION     DAXHRI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.074"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(99..1190)
                     /locus_tag="ACFM75_001276"
     CDS             complement(99..1190)
                     /locus_tag="ACFM75_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6255817.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGRVLAGTGMSNTVKHLAGIEKHNLQAERADGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNRYLDDGYDSLKARLD
                     GAWAETERLAGLAWNNTPDTLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            complement(1325..1873)
                     /locus_tag="ACFM75_001277"
     CDS             complement(1325..1873)
                     /locus_tag="ACFM75_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6255818.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            complement(2028..2399)
                     /locus_tag="ACFM75_001278"
     CDS             complement(2028..2399)
                     /locus_tag="ACFM75_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC6255819.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            complement(2455..2562)
                     /locus_tag="ACFM75_001279"
                     /pseudo
     CDS             complement(2455..2562)
                     /locus_tag="ACFM75_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2694..4385)
                     /gene="dld"
                     /locus_tag="ACFM75_001280"
     CDS             complement(2694..4385)
                     /gene="dld"
                     /locus_tag="ACFM75_001280"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC6255820.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEESRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            complement(4552..4648)
                     /gene="ffs"
                     /locus_tag="ACFM75_001281"
     ncRNA           complement(4552..4648)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFM75_001281"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4909..8742
                     /locus_tag="ACFM75_001282"
     CDS             4909..8742
                     /locus_tag="ACFM75_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC6255821.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETIQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLACWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKHTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKARYGSA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKHQVDPKHTFNRHKLMKGSDLSNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLANSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKYILGENWLDE
                     FVKKANNGGVEKVLL"
BASE COUNT         1908 a         2401 c         2460 g         2034 t
ORIGIN      
        1 gggtttctgt tttttccgat ggattcctgt tgcgttgggg gctggattcc cgctttcgcg
       61 ggaatgacgc ggtgggggtt tcggtttttt ccgcctgttt attttgcggc ttcgattgcc
      121 gctatttctt ttcggaggtg tttgatcgcg ggggttacga tggcgacaaa cattgcttcg
      181 cggacgcgcc tttgggcggg actgcgcatc aggtagcctt ttgcgccgga atgcagggcg
      241 gcggattggg cggcggcaag tgccagtacg gcggcggctt cgcgcagctt gagggtcgct
      301 aatgtgtccg gcgtgttgtt ccatgccaaa ccggcgagcc gttcggtttc tgcccatgcg
      361 ccgtccaacc ttgctttgag gctgtcgtag ccgtcgtcga gatagcggtt gacttcggcg
      421 ttgacgatgt tggcgaggcg gatgatgccg aggctgccgt caatcacgcc cgcgccgatg
      481 ccgatttgca ggaggatgaa acctgctttg atgctttgga tgtagtcggc aaactgttcg
      541 ggcgcggcga tgatgtcttc gtcggggata aatacgtctt tgaagttcag gctgaaggtg
      601 cgcgtacctt cgagggcgca aaattcgggg cagttttgca ggcttacgcc ttcccattgt
      661 ccgccggtga taaacatgac gtagccgccg ccgatttggg cggtatttgc ccagatgtgg
      721 tcttcgccga tgttggacac ccacggcagc gcgccgttga ctgtgtagcc gccgtctgcg
      781 cgctcggctt gaaggttgtg tttttcgatg ccggcaaggt gtttgacggt attggacatt
      841 ccggtacctg ccaatactct gccttgcagg atgtcggcga ggtatttgtc tttgacggct
      901 tggttgggcg tttggtgcag ataccacgcg caagccgcct gacaccacgc gctgaaggag
      961 gttgcgccgc attctttgcc gatttcgcgc aaaacggcga tttgcgttgc caagcctaag
     1021 ccgttgccgc cttcggcttc tgtcccgact gcgccgaatc cgccgattgc gccgagttcg
     1081 cgcataaatg cttcggggta gtatcctttg cggtcgatgt cgtccactat gggtttgagc
     1141 ttggttttga cgagttcggc aacgttggca atcagggttt gggcgttcat ctttgttcct
     1201 taaggtttgc gggggaatcg ggggcgcgcc tgatgcgcgg ctgctttccc tgccgcttgg
     1261 cggcttgttt ttgtttgagg agggggggtt gaaaaaccgc ccgatattcg ggcggtttgc
     1321 cgtatcaggc gtaagcctgc aattcggggt tgatttcggt ttgggcgagg ttgttgacgt
     1381 agttgcacag ggttgccaag gctacgccca tcacgacttc aactgcctgc tgctggttgt
     1441 agcccgcatc gaaaaatgct ttgagttcct cgtcggatac cgcgcctttt ttcgccatta
     1501 cggcttgggt gaaggcggcg agcgcgccga gtttggcatc gtcaaattcg cctgctgcca
     1561 aagcgcgcgc ggctttgacg gattgttcgg acaggagttt tttcagggtt gcgagtttgg
     1621 tgtgccctgc cacgcaaaaa ccgcattggt tggtacgggc ggcgatgatc tggatgactt
     1681 cgacttcgcc ggcggtcagg ctgttggcgg cgttgagctt gccgacttct tggtaaaacg
     1741 ccaaggcttc gggggcgttt gataatacgc cgataaggtt ggggataaag ccgttgtttt
     1801 gaagtaccgc ctcgacgcgc gctttggcgg cttcgggggc ggtttcgagg gtgtgtacgg
     1861 ttaaacgtgc cattttcttt ccttgttcgc aaatattggg tacgggcgca tggtatgcat
     1921 ttcggaacgg aataggaaag actgattggt tatgtgctgc aaacaaaagg ttataagaaa
     1981 tgccgtctga acatttttca gacggcatga tggaaaagaa aacgcgctta tcggcccccg
     2041 cgcccgaaat attgcgccaa tgcggcttgg ggattggctg cctcaaccgt ttcgggcaat
     2101 tcggtatagc cgcgcgataa aaggtggcgt gcataaaggc tgttgccctt tgcgccgaac
     2161 cagacggaaa ccgccaagcc caatacgtta aacgccgagt ctgtggcggg cgtgtcgccg
     2221 taaaccagtt gggcaaacac agccagcacg aatatcgcgc ccgtcagccc cagccccaaa
     2281 ccccacattc ttttgaaaca cgcccacagc gtgccgaaca agagccccgg ccatgaccag
     2341 ccttgtttga cggcttgggg cggcagggcg ggatgggtgt agattttgta tggtttcatc
     2401 gtgtttcctt ttcggttgaa accctgccct ttgggaaggt aggatcagac tttatagtgg
     2461 attaaattta aaccagtacg gcgttacctc gccttgccgt actatctgta ctgtctgcgg
     2521 cttcgttgcc ttgtcctgat ttaaatttaa tccactatat ttgggagggg cgcgcccgtg
     2581 tgtcggcata cggtttgaaa gcgattaccc gacggggaac ttcaaacccg acaatgccgt
     2641 ctgaacggcg ttttgccttc agacggcatt gcctgccttc aaagcggacg cgcttattcc
     2701 gcccagtttt tctttttgct ggttttgcct acgcccgggt tgaagctgtt ggtcgggtca
     2761 agtttgcggt aaaactgttt gagcgcgggt ttggcttcat acaaatgacc gacgttgtgt
     2821 tcggcgggat attgcgcgcc gcgttggtct aaaagatgca gcatttcgtg ttccaacgcc
     2881 atgcagtcgt tgcctttttt gatgatgtaa tcctgatgga aaacgtggca catgaaatgt
     2941 ccatagtaga gtttatggat gattttattg tcgatttccg acggcagttt ttcaaaccaa
     3001 tcgcggtcgt cgcggcgcag ggcgatgtcg agcgcgacca agtcttccac ttcgtcatca
     3061 tgcacggcgc ggtagcggat ggcggcggag gcgacggcga aacggtgcag catcgcggct
     3121 tgggtttctt cggcgttgca ctcgaaaaac gcgccgctgt ggtgtgcaaa atattctttt
     3181 aagaacgcgc gcgcctcatc cacgcctttc ccgcccattt tcagaatcag gtggtgttcg
     3241 tatttgtcgc ggtaatcgcg catggatttg ggcaggtggt cgggcaggta tttgctgacg
     3301 aactgcatgg ccttgtcgga aaaatgtttg ggcaggaagc tggctttttt gccgaacctg
     3361 tccacgcgcg ccttcaagtc aaataatttc ggcagttggt gcgtaccgaa ttttttgatg
     3421 acgtaaaacg tgtctttgcc gtacacgtcg gcaatgtcga aagcgtggcg gtggatgtat
     3481 tcgccggaaa caggcaggct ttcaaattcg cccaaggcgg cgcggcggat gtcggtcagc
     3541 tcgttgatgt cgttcgtgcc gatgtagaac acggcggttt gtttttcttg cgggaaggtg
     3601 tccaaacgga cggcaaaaac catcagtttg cccgcgcagc ccgaggcttc gtaatggcgg
     3661 gcggggtcgg cattgaaacg cgcggcggtc ggttcgtcca cttggcggac gtgttcgcaa
     3721 taggcgtggt cgtgcccttt gcccgcgtct tgtttgatgt ctttgttttg ataatgatga
     3781 ccttgcaggt tggtcaggat ttcttcgggc gtgtcgccca agtctatgcc caagtggttg
     3841 accagttcca acctgctttc ttcgttgatt tgggcgaaca gtgccatttc ggtgtaggcc
     3901 gggccgcgct gtaccaatgc gccgccggag ttgttgcaca cgccgcccaa gacggacgca
     3961 ccgatgcagg aagagccgat aaccgaatgc ggttcgcgtc ccaagggttt cagcagcaat
     4021 tcaagctggt tcagggtcga gccgggcagg cagacgactt gttcgttgtt gttgatggtt
     4081 tggatgatgt tcatccgcat ggtgttcaca atcacgatgt cgcggtcgta atcgttgccg
     4141 tcgggggtcg aaccgccggt cagtccggta ttcgccgcct gcgtaatcac aatcacgtct
     4201 gcttcgacgc acgcctgcag aattttccac atttccagaa tgcttccggg gcgaaccacc
     4261 gccaacgcct taccctcgcc gaagcggtaa ccttggcggt attgttcggt tttcgcgggg
     4321 tcggtaatga tgtatttttc gcctacggtt tgggtcagtc ttgacagtaa ttgtgatgcg
     4381 ctcatggcag tttccttaaa attgtcggca ggtgcattgc acattggaat tgttttcaca
     4441 ttgtagttat acgttatggc aaagtaaaga aaatgccgtc tgaacggctt tcagacggca
     4501 acggtgcgat acgggaacgc cggaacatca aagctccggc gtttcaaata gggcggcggg
     4561 ccaaaccccc ggcactggcg caccggagtg ggctgctggc ttccgcccct gacccggtgt
     4621 tccgatttgc catgcgggga gacccgcctc agagaaacgg cattataacg ggttttctga
     4681 aaaactcaac cgttttgata cggtcatacg ccggaaacac cacctaaaat ttatatttga
     4741 taatattgtc aacaatttct caaagcgtta ttttgtttct ataagggtat ttcctgtttc
     4801 ggcattgaaa aatatcaaaa attgaactac attatcgcct tttcaaactc gcctgaaacc
     4861 gaattttcag acggcattca aataaaaact gccaagcacg gacacaccat gaccacgact
     4921 actgcccctc agcgtattcg agaaattccc tataactata cttcttatac cgaccgcgaa
     4981 atcgtcatcc gcctactggg cgatgaggcg tggcaaatcc tgcaagactt gcgcggacag
     5041 cgcaaaaccg gacgttcggc acggatgctg tttgaagtgt tgggcgatat ttgggtggtc
     5101 gtgcgcaatc cgtatctggt cgatgatttg ctggagcacc caaaacgccg cgccgcgctg
     5161 gtgcgggaaa tgcgccaccg cttaaatgaa atccgcaaac gccgcgacga taatcggcaa
     5221 gtcgatgtgt tggttgccgc agcagaaaaa gcagtggagc gttttgatag cagttttgat
     5281 gaaaccatcc aaaaacgcca tcaaattttg gaacgcttaa gcaaaattac caagccgcac
     5341 aacatcatgt ttgacggatt ggcgcgcgta acgcacgtta ccgatgcgac cgactggcgc
     5401 gtcgaatatc cttttgtcgt cgtcaatccc gacacggagg cggaaatcgc gcctttggtg
     5461 cgcgccttaa tcgaattgga cttggttatt atcccgcgcg gcggcggcac gggttatacc
     5521 ggcggcgcgg tacctttgga cgcgaacagc gcggtcatca ataccgaaaa actcgacaag
     5581 catcgtggtg ttgaatacgt tgagctggcg ggcttggacg gcaagcatcc gattatccgg
     5641 tgcggagcgg gcgtagtaac ccgacgagtg gaagaaaccg cgcatcaggc aggtttggtg
     5701 ttcgccgtcg atccgacttc cgccgacgcg tcctgcgtgg gcggcaatgt ggcgatgaac
     5761 gcgggcggca aaaaagccgt actgtggggg acggcgttag acaaccttgc ctgctggcag
     5821 atggtcaacc cgcagggtga atggctgcgc atcgagcgcg tgcgccacaa tttcggcaaa
     5881 atccacgacg aagaaaccgc cgtattcgac gttcacacgc tggattcaga cggcctcaat
     5941 atcgttaaaa ccgaacgctt ggaaattccc ggccacaaat tccgcaaagt cggtttgggc
     6001 aaagacgtta ccgacaaatt cttgagcggc ctacccggcg tgcagaaaga aggcacggac
     6061 ggcatcatca ccagcgtcgc cttcgtgttg cacaaaatgc cgaaacacac gcgcaccgtg
     6121 tgtatggagt ttttcggtac ggtcgccacc gctacgcctt ctatcgtcga aatccgcgat
     6181 ttcctgcttg cccacgacag cgtgcggctg gcgggtttgg aacatttgga ctggcgttac
     6241 gtccgcgccg tcggctacgc aaccaaagcg gcgggcaagg gacggccgaa aatggtcttg
     6301 ctggcggacg tggtttccga cgacgaagcc gccgtagagg cagccgccga acacatttgc
     6361 gaacttgccc gcgcccgcga tggcgaaggc tttatcgccg tgtctcccga agcgcgcaaa
     6421 accttttggc tcgaccgcag ccgcaccgcc gccatcgcca agcacaccaa cgcctttaaa
     6481 attaacgaag acgtggtcat cccactcgaa aggctcggcg agtattcaga cggcatcgaa
     6541 cgcatcaaca tcgagctttc catccaaaac aaactcaaac tctgtgccgc attggagcaa
     6601 tatctttcgg gcaaactccc catcgacaaa atgggaacag acctgccgac cgccgaactg
     6661 ttgggcgaac gcggcaaaca cgcccttgcc cacgtttccg ccgtcaaagc gcgttgggaa
     6721 tggctgcttg ccaacttgga cgcgccgctt gccgactata aagcacgcta tggctcagcc
     6781 gtccacgccg cgcccgaagc caaagacaat gaaagctgct ttaccgcatt ccgcgatttc
     6841 cgcctgcgcg tgtctgtcaa agcggacgta atgaagccgc tttctgaaat cttcagcggc
     6901 aaaaccgaca ccaaaattat ccaaggcttg ggaaaaatcc acgcaaaaac cgtacgcagc
     6961 cgcgtcttcg tcgccctgca tatgcacgcc ggcgacggca acgtccacac caacatcccc
     7021 gtcaattcgg acgatgccga aatgcttcag acagcctacc gttcggtcga acgcattatg
     7081 aaaatcgccc gttcgctcgg aggcgtgatt tcgggcgaac acggcatcgg catcaccaag
     7141 ctcgaatttt tgaccgatga agatttgcaa ccgttttgga actacaaaca ccaagtcgat
     7201 cccaaacaca ccttcaaccg tcacaaactg atgaaaggct cggacttaag caacgcctat
     7261 acgccatcct tcgagctttt aggtgcggaa tcgttgatta tggaaaaatc agacctcggt
     7321 accatcgccg attccgtcaa agactgcctg cgctgcggca aatgcaaacc cgtctgctct
     7381 actcacgttc cgcgtgccaa cctgctgtac agtccgcgca acaaaatcct cggcgtaggc
     7441 ttattgaccg aagccttctt atacgaagaa caaacccgcc gcggcgtttc catcaaacac
     7501 tttgaagaac tcatggacat cggcgaccac tgcaccgtgt gccaccgctg cgtcaaaccc
     7561 tgccctgtca acatcgactt cggcgacgtt accgtagccg tccgcaacta ccttgccaat
     7621 tccggccaca aacgctttgc gccggcggct tcaatgggta tggcattcct aaacgcgacc
     7681 ggtcctaaaa ccatcaaagc cctgcgcgcc gcgatgatac agaccggctt ccccgcgcag
     7741 aactttgcct acaaaatcgg caagcttctt ccgatcggca ctaaaaagca aaaagccgaa
     7801 cccaaagcca ctgtcggcaa agccccgatt aaagaacagg ttatccattt catcaaccgc
     7861 ccactgccca aaaacgtacc cgccaaaaca ccgcgctcct tattgggcat cgaagacggc
     7921 aaaagcatcc ccatcatccg caaccccgcc gcgcccgaag atgccgaagc cgtgttctac
     7981 ttcccgggtt gcggctctga gcgtctgttc agccaaatcg gacttgccgt tcaagccatg
     8041 ctctggcacg tcggcgtaca aaccgtcctg ccgcccggct atatgtgttg cggctatccg
     8101 caagacgcgg gcggcaacaa ggctaaagcc gaagaaatga gcaccaacaa ccgcgtggct
     8161 ttccaccgta tggcgaacac cctcaactac ctcgacatta agaccgtcgt cgtcagttgc
     8221 ggcacttgtt acgaccagct cgaaaaatac cgctttgaag aaatcttccc cggctgccgc
     8281 atcatcgaca tccacgaata cctgctcgaa aaaggcgtga aactcgacgg cgtgaaaggt
     8341 cagcaatacc tctaccacga cccctgccac acccccatca aaaccatgaa cgccacccaa
     8401 atggccagca gcctgatggg gcagaaagtc gttttaagcg accgctgctg cggcgaatcc
     8461 ggtatgtttg cagtcaaacg accagacatc gccactcagg tcaagttccg caaacaagag
     8521 gaaatcgaga aaaacctcaa agagctgccg cagggcgaac ccgtcaaaat gctgacctcc
     8581 tgccccgcct gcctgcaagg cttgagccgc tacgccgacg acaacaatat gcctgccgac
     8641 tacatcgtca tcgaaatggc gaaatacatc ctcggcgaaa actggctgga tgagtttgta
     8701 aaaaaagcca acaacggcgg tgtagagaaa gtgttgctgt aacaacggac acggaaatgc
     8761 cgtctgaacg ccggaagcct tcagacggca tcgtttggac caa
//
LOCUS       DAXHRI010000075         8740 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXHRI010000075 DAXHRI010000000
VERSION     DAXHRI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.075"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(310..1206)
                     /gene="argB"
                     /locus_tag="ACFM75_001283"
     CDS             complement(310..1206)
                     /gene="argB"
                     /locus_tag="ACFM75_001283"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC6255822.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1376..2506
                     /locus_tag="ACFM75_001284"
     CDS             1376..2506
                     /locus_tag="ACFM75_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC6255823.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2577..3428
                     /gene="lgt"
                     /locus_tag="ACFM75_001285"
     CDS             2577..3428
                     /gene="lgt"
                     /locus_tag="ACFM75_001285"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC6255824.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3511..4173)
                     /locus_tag="ACFM75_001286"
     CDS             complement(3511..4173)
                     /locus_tag="ACFM75_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC6255825.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4268..6061)
                     /locus_tag="ACFM75_001287"
     CDS             complement(4268..6061)
                     /locus_tag="ACFM75_001287"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC6255826.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDSENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6307..7416
                     /gene="proB"
                     /locus_tag="ACFM75_001288"
     CDS             6307..7416
                     /gene="proB"
                     /locus_tag="ACFM75_001288"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC6255827.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKLRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7429..8691
                     /locus_tag="ACFM75_001289"
     CDS             7429..8691
                     /locus_tag="ACFM75_001289"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC6255828.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYVDKDADLEKAIRIVRNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYINVSTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1977 a         2461 c         2284 g         2018 t
ORIGIN      
        1 aatctaaaga aacctttttt cccgataagt ttccgtgccg acaggtctag attcccgcct
       61 gcgcgggaat gacgaaattt caaagttatg gcgttatcgg aaaaacaaaa aatcaaagcc
      121 ggagaattta tcccaaacaa ccggatttca aaaaaaccag attcccgcct tcgcgggaat
      181 gacggatttt aggtttctgt tttggtttct gttttcgcgg gaataacaac ttagaaattg
      241 cccgaaaccc caaaaacaga aaccggacaa acgggttttc cgccaaagcc ggcattttcc
      301 gactttgctt caggcatctt ccccaccgcc caaaatcatc gaaccgatac cggcatcggt
      361 aaagatttcc agcaaaagcg cgttgggcac cctgccgtcg atgatatgcg tggctttcac
      421 gccgttgacg gcggcttcga ccgcagaagc gattttcggc agcataccgc catacagcgt
      481 gccgtcggca atcagttcat caatccgttt cggcgtgagt ttggtcagca gattgcccgt
      541 tttgtccatc acaccggcga tattcgtcat catcaagagt ttttcggcgt tcaattcttc
      601 cgccaatttg cctgctacca aatcggcgtt gatgttgaac gcttcgcctt tttcacctac
      661 gccgacgggg gcgacgacgg gaatgcagcc acgttctatc agccctttaa ccaaaccggt
      721 atcgatgctt tccaccgtac cgacctgtcc gatgtccacg ccattctgtt cgggcgtatc
      781 gatcaaaagt ttcttcgcct taatgaaatg gtcgtcgcgt ccgcttacgc cgaccgcgtg
      841 tccgccatat gtgttaatca tcgacacgat ttctttattg acatgcccgc ccaacaccat
      901 ttcgacaata tccatcgcct ctttgtcggt aacgcgcatt ccttggacaa actcaccctt
      961 tttgccgact ttttcaagca tcgcattgat ctgcggcccg ccgccgtgaa cgatgacggg
     1021 atgaatgccg accagcttca gcagcacgac atcgcgggca aacccttctt tcaaggcagg
     1081 ttcggtcatc gcgttgccgc cgtatttgat gacggcgacc gaaccggaaa accggcggat
     1141 gtaaggcagc gcttcggcaa ggatacgcgc cttgtcggcg gcggaaataa tgttttcaga
     1201 ctccataacg gctccctatt gttgacaatt tcggcttatg ttaacgccaa acgccgtctg
     1261 aaacaatctt cagacggcat ccggcagact ttcgagcagg ttgaagcctt gcgatacggg
     1321 cggtaaaatc gcggtttatc cggttcgaga aaccggcgac agcaaggact ttcccatgat
     1381 taaaatcagc acccgcttcg atgccggctc ggtcgtcgtc aaagacctga ccgatccttc
     1441 caacatccgc ctcgccctgc gtcccgacaa cgcctccgat tttgcacaat ggttctactt
     1501 ccggctgcaa ggcgcggcat atcaaaactg catcatgcat tttgaaaacg cggcagaagc
     1561 cgcctacccg aaaggctggg aaggctatca ggcgtgtgcc tcatacgacc gccgcaactg
     1621 gttccgcgta ccgacttcct acgaaaacgg cgtactgacc gtcaatcata cgccgctgtc
     1681 caacagcgtg tattacgcct atttcgaacc ttactccgaa gaacagcatt taaacctcct
     1741 cggcgacgcg caaggcagcg gcctgtgccg catcgacgac ttgggcagca ccgtgcaagg
     1801 gcgcgacatc aatctgctga ccatcggcaa tcaggtcgaa agcgatctga aaatctggat
     1861 taccgcccgc cagcatccgg gcgaaaccat ggcggaatgg tttgtcgagg ggctgctcgg
     1921 caggctgctc gatccgcaag acccgacagc gcgcaccctg ctcgaccgcg ccacgttcta
     1981 tatcgtcccc aatatgaacc ccgacggttc ggcactgggc aaccttcgta ccaacgccgc
     2041 cggtgcgaac ctcaaccgcg aatgggaaaa tccgactttg gaaaaaagcc ccgaagtgtt
     2101 cttcgtgcgc gaaaaaatgc tggaaaccgg cgtggatttg ttcttggaca tccacggcga
     2161 cgaagggctg ccctttatct ttgttgcagg aactgaaggc gtgccaaact acaatccgcg
     2221 catttccgcg ttggaaacac agttcaaaac cgcccttttg aacgccagcc ccgatttcca
     2281 agacgaatac ggttacgaaa aagatgcgcc cggtcaggca aacatgacct tggcaacaaa
     2341 ctgggtcggc aaccgtttca actgccttgc ctatacgttg gaaatgccgt tcaaagacaa
     2401 tgccaacctg cccgacgacg atttcggatg gaacggtcaa cggtcgttgc gcttgggcga
     2461 agccgcgttg tccgccatcc tcaacatcat cggcgatttg cgctgaaacc cttattgcag
     2521 aaatgccgtc tgaagcatca tgcctttcag acggcatttg aaaacgaaag cccgttatga
     2581 ttacccatcc ccaattcgac cccgtcctta tcagtatcgg cccgcttgcc gtccgctggt
     2641 atgccctaag ctacatcctc ggatttattc tttttacctt tctcggcaga aggcgcatcg
     2701 cgcaaggctt gtccgttttt accaaagaat cgctcgacga cttcctgaca tggggcattt
     2761 tgggcgtaat tttgggcggg cgtttgggtt acgtcctgtt ttacaagttt tccgactacc
     2821 tcgcccatcc gcttgatatt ttcaaggtat gggaaggcgg aatgtcgttc cacggcggct
     2881 ttttgggtgt agttattgcc atatggttgt tcggtcgcaa acacggcatc ggcttcctca
     2941 aactgatgga cacggtcgca ccgctcgttc cactgggtct cgcttcggga cgtatcggca
     3001 acttcatcaa cggcgaactt tggggacgcg ttaccgacat caacgcattt tgggcaatgg
     3061 gcttcccgca ggcgcgttac gaagacctcg aagccgccgc gcacaatccg ctttgggcag
     3121 aatggctgca acaatacggt atgctgccgc gtcatccctc gcagctttat cagtttgcac
     3181 ttgaaggcat ctgcctgttc gccgtcgttt ggctgttctc taaaaaacag cggccgaccg
     3241 gacaagtcgc ctcgctcttc ctcggtggct acggcatatt ccgcttcatt gccgaattcg
     3301 cacgccaacc cgacgactat ctcgggctgc tgaccttggg gctgtcgatg gggcaatggt
     3361 tgagcgtccc gatgattgtt ttgggtatcg tcggctttgt ccggttcggc atgaaaaaac
     3421 agcactgacc gcctgtgaaa aataccgtct gaaacatgga aatccggttt cagacggcat
     3481 ttttacgttt ggttcatgga aacaaacgct tcaattctta ccgcaacacg ccttgtattt
     3541 tctgccgctg ccgcacggac agggatcgtt cctgcctgtt ttttcgcctt ccctgcggac
     3601 ggtttgcggt ttgttgatga ccgcctgcca gtagcggtag atatccgcca atgcataagg
     3661 caactcggat tccagttccg ccagctcgcc ttctgtgaat tgcagacgga tagtgccgtt
     3721 gtcctcttca tcgtaaatac cgcccaatgc catgatggga taaaacaact cttcaaacgc
     3781 ttcatcatcg acggcttcaa accaatcggt cggcacaata tccaaaccgt aaagataagc
     3841 attgcaccat gtgtaaaaat cgctgccgcc gtcttcgttt tcatacagcc acaaatcggg
     3901 cagtttttta tccgacatcg cggcggttgt ttccatcgcc attgccaaaa ccagccgttc
     3961 gatttcggaa cgttcggcgg cggtaaattg cgattcgtcg cccaacactt cgggcagcca
     4021 gtcgagcggt gtcaatttgt ccggcccgct caacagcgcc gtcataaaac cttgaacctc
     4081 gtcgcaacgc atcgtgttgc cttgttcgct tttggcatcc aacaattcgc tcaaccgccg
     4141 tttggatgct tcggtaaatt tttgggaatc catgattttc cttttcaaat gggttttgcg
     4201 ccctattatc gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt
     4261 ttcacgctca aatcgtaccg ctgccttgcg ctttcggttt ggcggcacta aattccagtt
     4321 tgctcaaggc ggaaaggtag gctttggcgg tagcgaccaa aacgtcggta tccgcgccct
     4381 gaccgttgac gacgcggttg ccgcgcgcca gacggacgct ggtttcgccc tggctttccg
     4441 taccttgcgt gacggcgttg acggaataaa tctgcaaagc cgcgccgctt tgcgccacgc
     4501 tttcaatcgc tttgaaaatc gcatccacgg ggccggagcc tgtggcggaa gcgcgttttt
     4561 cttcgccttt gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga
     4621 ttttttggga gatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca
     4681 gtgcgtgcag gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg
     4741 caaacgcggc gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg
     4801 ttttgaaggc gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga
     4861 ccgattcggc ggacataatc tcgtaagttt cgcggtgttt cagcacccca tcctgatgga
     4921 tgcccgattc atgcgaaaag gcattggcac cgacaatggc tttgttgggc tgcacgggat
     4981 agcccgtaat ggtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg
     5041 tttccaagcc gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag
     5101 cattgcctgc acgttcgccc aagccgttga cagtacattc cacctgacgc gcgccgcctt
     5161 ttaatgcggc aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc
     5221 aaacgacttt gccgccgttg ggcgttttgg caatcagttc gcggaaaaat tcttcggttt
     5281 tgtacgggat tgaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa
     5341 tcaccgcgcc gcagatttcg gcaagaaaat cgatttccga acgcaacgcg tcttcgcagg
     5401 aaaattccac atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa
     5461 tcacctgctt cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga
     5521 aggtgtggat gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc
     5581 gctcgatggc gcgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat
     5641 tgaccgcctc gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac
     5701 ccaatttttc cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg
     5761 gcgattgttc gccgtcgcgc agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca
     5821 tttctgttct ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc
     5881 cttgcgattc ggcaagcgcg gtcagctttt gcagttgcgc caaaggcagc gacccgcgcg
     5941 tgcgccattt atagatggcg gcggtcgtcg cggcattttc ggaatcgtgc tgtttcaacg
     6001 cttcggcaag cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca
     6061 tggtagtcct tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca
     6121 ttttggacaa aacggcaata taaatttgaa aaatctcaat aagccgccat agattttatt
     6181 aattttatac attttgtcta ttttatcgca gacgcggcgt gattgttgac aattgcggca
     6241 gaacagggca aattcggcgg cacaaggcgg caaacgagac aggacaaacc aacaaggacg
     6301 gaaacaatga aatacaaaag aatcgtattt aaagtcggca catcttcgat tacccattcg
     6361 gacggcagtc tctcgcgcgg caaaatccaa accatcaccc gccagcttgc cgcattgcat
     6421 catgcgggac acgagctggt cttggtgtct tccggcgcgg ttgcggcagg gttcggtgcg
     6481 ctgggtttca aaaaacgtcc ggtcaaaatc gccgacaaac aggcttccgc cgccgtcggg
     6541 caggggctgc tgatggaaga atatacggca aacctgtctt cagacggcat cgtgtccgca
     6601 caaatcctgc tcagccgcgc cgactttgcc gacaaacgcc gctaccaaaa tgccggcggc
     6661 gcactttccg tgctgctgca acgccgcgcc gtccccatca tcaatgaaaa cgatacggtt
     6721 tcggttgagg aattgaaaat cggcgacaac gacacattga gtgcgcaagt ggcggcgatg
     6781 atacaggcag acctcttggt gctgctgacc gacatagacg gtctttacac cggcaacccg
     6841 aacagcaatc ccgatgccgt acggctggac aaaatcgaac acatcaacca tgaaatcatc
     6901 gaaatggcgg gcggctcggg ttcggcaaac ggcacaggcg gtatgctgac taaaatcaaa
     6961 gcggcgacga ttgcgaccga gtccggcgta ccggtctata tctgttcctc gctcaaaccc
     7021 gatgcacttg ccgaagcggc agataatcag gcggacggct cgtttttcgt cccccgtgcc
     7081 aaaggtttgc ggacgcagaa gcaatggctg gcgttctatt ccgaaagcag gggcggcgtt
     7141 tatgtggacg aaggtgcgga acacgctttg tccgaacagg gaaaaagcct gctgatgtcg
     7201 ggcattgccg gaatcgaagg gcatttttcc cgtatggaca ccgtaaccgt gtacagcaag
     7261 gcaaccaaac agcctttggg caaagggcgc gtcctgttcg gctctgccgc cgccgaagac
     7321 ctgctcaaat tgcgtaaggc gaaaggcgtg ttcatccatc gggacgactg gatttccatc
     7381 acgcccgaaa tacgcctgct tctgaccgaa ttttagaaaa ggaaacccat gtcaaacaca
     7441 caaaaacagc ttgcccttgc caaagcggca aaaaaatccg tcaacacggc ggatacggaa
     7501 gaaaaaaacc gcgcgctgtt tgctatggcg gacagcttgg aagcggcggc ggcagatatt
     7561 ttggcggcaa accgtcaaga cttggaagcc gcggcaggca acattcccga aagcatgacc
     7621 gaccgccttt tgttggacgg caaacgcatt tgcgcgatgg cggacggcat cagggcggtt
     7681 gccgcgctgc ccgaccccgt gggcgaaata ctggaaacct cgactttgcc caacggcttg
     7741 gaaatcgtca aaaagcgcgt ggcgatgggc gttatcggca ttatttacga aagccgcccg
     7801 aacgttacgt ccgatgcggc ggctttggca ctgaaaagcg gcagcgcggt cgtactccgc
     7861 agcggcaaag atgcattcca atccgcacgc gccatcgttg ccgccctgaa aacggggttg
     7921 gcacaaaccc gcatcgaccc cgacgcgctc cagttgattg aagacaccgg gcgtgagggc
     7981 agttacgaaa tgatgagggc gaaagattat ctagacctgc tgattccgcg cggcggggcg
     8041 gggctgatac gggcggtggt tgagaatgcc gtcgtgccgg tcattgaaac ggggacgggc
     8101 attgtccaca tctacgtcga taaagatgcg gacttagaaa aggcaatccg cattgtccgc
     8161 aacgccaaaa ccagccgtcc gtccgtgtgc aactcgatgg aagtgctgct ggtgcatgaa
     8221 gacattgctg ccgacttcct gcccaagctc gaacggctgt tggttcgcga ccgtatagaa
     8281 gccggactgc cgcccgtccg cttccgtttg gatccgcagg cggcgcggca tatcggcggc
     8341 gaagcggcgg gtgcagacga tttcgatacc gagtttttag actacatcct cgccgtgaaa
     8401 accgtcgctt cggtcgaaga ggcggtcggg cacatcgaaa cgcacggcac gcaccattca
     8461 gacggcatcg ttaccgaaaa ccgccacgct gcggactatt tcacgaccca tatcgattct
     8521 gccgccgtgt atatcaacgt gtccacgcgc tttaccgacg gcggcgaatt cggcttgggt
     8581 tgcgaaatgg gcatctccac gcaaaaactc cacgcccggg gcccgatggg tttaaaagag
     8641 ctgacgagtt acaaatacat cgtacaaggc acgggacagg ttagggaata atccgaattc
     8701 aaacgtctga tatagtggat taactttaaa ccgatacggc
//
LOCUS       DAXHRI010000076         8697 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXHRI010000076 DAXHRI010000000
VERSION     DAXHRI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.076"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            191..1726
                     /locus_tag="ACFM75_001290"
     CDS             191..1726
                     /locus_tag="ACFM75_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6255829.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLNIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     DEK"
     gene            1723..1815
                     /locus_tag="ACFM75_001291"
     CDS             1723..1815
                     /locus_tag="ACFM75_001291"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC6255830.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="ACFM75_001292"
     CDS             complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="ACFM75_001292"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC6255831.1"
                     /translation="MTLFCEQVPYPRLAEEFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELVRVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAERLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDTLVA
                     EGIVLEHLDLGGGVGIVYQDESVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGGGYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3106..3276)
                     /locus_tag="ACFM75_001293"
     CDS             complement(3106..3276)
                     /locus_tag="ACFM75_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6255832.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFM75_001294"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFM75_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC6255833.1"
                     /translation="MMTESEFIRMSEELFEHIEDQIDENGWDFDCQFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3697..4713
                     /locus_tag="ACFM75_001295"
     CDS             3697..4713
                     /locus_tag="ACFM75_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFC6255834.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4739..5158
                     /locus_tag="ACFM75_001296"
     CDS             4739..5158
                     /locus_tag="ACFM75_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC6255835.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRILDDELLDKILSRKST"
     gene            5180..5686
                     /gene="luxS"
                     /locus_tag="ACFM75_001297"
     CDS             5180..5686
                     /gene="luxS"
                     /locus_tag="ACFM75_001297"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC6255836.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMATPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNRNDDLA
                     LDESLLNA"
     gene            5832..8624
                     /gene="polA"
                     /locus_tag="ACFM75_001298"
     CDS             5832..8624
                     /gene="polA"
                     /locus_tag="ACFM75_001298"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC6255837.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     ITTEAQFAALLDKLAQADAIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIEQATEYAAQDADFALR
                     LEAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVRNCLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
BASE COUNT         2037 a         2258 c         2285 g         2117 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg gatccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtattcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgcct tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcaatatt ttcggttggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagacgaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttacag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta tccgccgccg tcaaccaata cctccgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat gctggcaccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcacaaatc
     2101 gggccgacga tgttggcggt cagcgtcgcg atgtttttgg tttcgaccgc ttcgatgtgg
     2161 tggtaggcat cataaagtgc cggacgcatt aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgtg tttgacgaat tcaacgcgcg tcaataacgc acctgcgtta
     2281 ccgaccaagc tacgacctgg ttcgaggatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaaccgctt gggcatatgc gcccaaatcg gggacgcttt cgtcttgata aacgatgccg
     2401 acaccgccgc ccaagtccaa atgttccaaa acaatgcctt cggcgacaag agtgtcaacc
     2461 aaaatcaaaa tgcgttcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg gcggtaggct
     2581 tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagtcctgt ggagatgtag
     2641 ggatgggttt tcgcatcgac atcggggttg acgcgcaggg agacggacgc ggttttgccc
     2701 aagcgttcgg caaccttttg aatgcggtcg atttcgggaa tgctttccat attgaagcat
     2761 ttcacgcccg cattcagcgc aaactcgatt tccgcctcgc ttttgcctac gcctgaaaag
     2821 atggttttcg ccgcgtcgcc gcctgccgcc aaaacgcgta ccaattctcc gccggacaca
     2881 atgtcgaaac cgctgcccag agaagcaaag tgtttgatga tgctcaggtt gccattggct
     2941 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggcg tgccgaattc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaatagg gtcatgtttt cgttttcatt tttgggtttg
     3121 tggagcggat tgtggtttgc tttgaagttg caaaccggtt tggattacgc cgaaacgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg agagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggtat cttaaacaaa aaacacgcaa aaagccatga tgaccgaaag
     3361 cgaattcatc cgcatgagcg aagaattgtt cgaacacatc gaagaccaaa tcgacgaaaa
     3421 tggctgggat ttcgactgcc agtttgccgg aaacgtcttg accatcgaag ccggagacgg
     3481 cacgcaaatc atcgtcaacc gccacacgcc caatcaggaa ttgtggattg ccgccaaaag
     3541 cggcggctac catttcgccg agcaaaacgg caaatggctg gcaacacgcg acagccgcga
     3601 tttctacgac gttttaaacg aagcactgag cgcggcttcg ggcgaagcgg tagagattga
     3661 ggaattgtga tttgggtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
     3721 ggacttatat ttatcgcgat tatcgctata cttgctaact atttaggaaa cactgatttt
     3781 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
     3841 aataccattt atccgcaatt ttcaacacaa gtggaaaaag gcgttttgtt tgccaaaggc
     3901 gcgcttcttc gcactggcat tgtgctgtat ggtttccgcc tcacttttgg cgatattgcc
     3961 gatgtaggat taaatgctgt cgttaccgat gcaatcatgc taatttcaac cttctttttt
     4021 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
     4081 gcaggttgca gcatttgcgg tgcggcagca gtgatggcgg cagagcctgt taccaaagca
     4141 gaatcccata aagtttcagt ggcgattgcc gtagtggtca ttttcgggac gcttgctatt
     4201 tttacttacc ccttgttcta cacgtggtca caacatttaa ttaacgccca tcaattcggt
     4261 atttatgttg gttctagtgt acacgaagtg gctcaagttt atgcgattgg ggaaaatatt
     4321 gatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
     4381 ccctttttat taatgctttc ttggttatta acacgtagta atggagtatc agaaaataca
     4441 tcacacaaaa ttacaattcc ttggtttgct gtacttttta ttggtgttgc catttttaat
     4501 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
     4561 ttaatttcat caatggctgc gcttggctta acgacgcaag caagcgcaat caaaaaggca
     4621 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
     4681 ttagtgaact atggaatatc aaaattaata taaaattcac taaagagagc gttacccaat
     4741 ggcacaatta ccgctatatc tgacttctga aatcaaagac tttactgtcg gcacgcctaa
     4801 agttttggaa tcattttcca aacatatccc ttatggtgtt gtctttgaag acgacggcga
     4861 cacgggctac ttctatgccg cttcgcaaga cgggatttta gatgccttgc acatctacaa
     4921 tgtcgaagat gtatccgaca aacatatccc caatcatgtc ttgattttat gggatgatgc
     4981 ctgcaccata gccgcattgt gtatcaacga ctacattcat gccgtctatg attttgtcga
     5041 acaggcagga tattgccgca acggcttccc tgaagcaggc ggcgaatggg tgaaagtcga
     5101 aaaccgcatc ttggacgatg aattgctgga caaaatccta tcccgaaaat ctacataacc
     5161 tactcaaaag gataacaaaa tgcccctgtt agacagtttc aaagtcgatc acacccgtat
     5221 gcatgccccc gccgtacgcg tggcgaaaac catggccacg cccaaaggcg acaccattac
     5281 cgtgttcgac ctgcgctttt gcgttcccaa caaagaaatc ctgcccgaaa aaggcataca
     5341 cacgctggag catttgttcg ccggttttat gcgcgaccac ttgaacggca acggcgtgga
     5401 aatcatcgac atttccccga tgggctgccg caccggtttc tacatgagcc ttatcggcac
     5461 gccttccgaa cagcaggtcg ccgatgcgtg gctggcttcg atgcaggatg ttttgaatgt
     5521 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
     5581 ctcgcttgcc gaagcgcagc aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtcaa
     5641 ccgaaacgac gatttggctt tggatgaaag cctgctgaac gcctaatccg ccaaaaatgc
     5701 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatccgg ggttgtgcgg
     5761 gggcgggttt taagccggca tcgtccttcc ctattttttt ctgtccctta tcggttttaa
     5821 gcgggttttt tatgtccaac agacctacac tcctcctcgt tgacggctct tcctatctct
     5881 accgcgcgta tcacgcgatg gggcaaaacc tgaccgcccc cgacggcgcg ccgacgggcg
     5941 cgctgtatgg cgtattgaat atgttgcgcc gtttgcggtc ggaatatccg cacgattatt
     6001 gcgcggtggt ttttgatgcg aaaggcaaaa atttccgcca tcaaatgttt gaagaataca
     6061 aggcgacgcg cccgccgatg cccgacgatt tgcgcccgca ggcggaagca ctgccggatt
     6121 tagtgcgcct gacgggctgg ccggtattgg tgattgggca agtggaggcg gacgatgtga
     6181 tcggcacgct ggcgaaacag ggggcggaac atggtttgcg agtcattgtt tcgaccggcg
     6241 ataaggacat ggcgcagttg gtggatgagc gcgttacgct ggtgaacacg atgagcggag
     6301 aaacgctgga tattgaaggc gtgaaggcaa aattcggcgt gcgccccgac caaatccgcg
     6361 attatctcgc gctgatgggc gacaaggtgg acaacgtgcc gggcgtggaa aaatgtggcc
     6421 cgaaaacggc agtgaagtgg ctggaagcct acggctcgtt gcaaggcgtg atggaacacg
     6481 cttcggaaat caagggcaaa gtgggcgaga atctgcgtgc cgccctgccg cgcctgccgc
     6541 tgtcgtatga tttggtaacg attaaaaccg atgtggactt gcacgccgag ctttcagacg
     6601 gcatcgaaag cctgcgccgt actacgccga aatgggcgca gctggttgtc gatttcaaac
     6661 gctggggctt ccgcacctgg ctgaaagaag cggaatcaaa catgaatacc ggctcgaccg
     6721 atgatttgtt cggcagcgac agcatcggcg agcaggcggc tttgaatgcg gaaatgccgt
     6781 ttgaaaaaca agccgaaaaa gccaccgccc ccgaaaagct ggattatcaa gctattacca
     6841 ccgaagcgca gtttgccgcc ttgttggaca aactggctca ggcagacgca atcggcatcg
     6901 atacggaaac tacgtcatta gacgcgatga acgccgcgct ggtcggcatc agcatcgcgt
     6961 tccaagcggg agaagcggtt tacatccccg taggccacag cctgaccgcc gcgcccgaac
     7021 agcttgattt gcaggacgta ttaggtcgtc tgaagccgca tttggaaaat cccgccctga
     7081 aaaaaatcgg gcaaaacctc aaatacgacc aacacgtttt cgccaactac ggcatcgccc
     7141 tgaacggcat tgccggcgac gccatgctcg cttcttacat catcgaaagc catctcggac
     7201 acggtttgga cgaattgtcc gaacgctggc tcggcttgga aaccattacc tacgaatcgc
     7261 tgtgcggcaa aggcgcgaag caaatcggtt ttgctgacgt cgccatcgaa caggcgaccg
     7321 aatacgccgc ccaagacgcc gatttcgccc tgcgcctcga agcacacctg cgcgcgcaaa
     7381 tggacgccaa acagcttgaa atgtatgaaa aaatggagct gcccgtcgcg caggtattgt
     7441 ttgaaatgga acgcaacggc gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg
     7501 aactcggcgc cgagctgatg aagctcgaac aggaagccta cgccgccgca ggccagccgt
     7561 tcaacctcaa ctcgcccaaa cagctgcaag aaatcctgtt cgacaaaatg ggcattccta
     7621 ccaaaggcct gaaaaaaact gccaaaggcg gcatttccac caacgaagcc gtgcttgaac
     7681 agctcgcgcc cgactaccca ctgcccaaaa tcatcctgca aaaccgcagc ctggcgaagc
     7741 tcaaatccac ctacaccgac aaactgcccg aaatgatttc ccccaaagac ggccgcgtgc
     7801 ataccaccta cgcccaagcc gtcgccatta ccggccgcct cgccagcaac aaccccaatc
     7861 tgcaaaacat ccccatccgc accgccgaag gccgccgcgt acgccgcgcc ttcaccgcac
     7921 cgcaaggcag cgtcatcgtt tccgccgact attcccaaat cgagctgcgc atcatggcgc
     7981 acctctccgg cgacaaaacc ctcatcgccg cgttccaaaa cggcgaagac gtacaccgcc
     8041 gcaccgccgc cgaagtgttc ggcactgcgc ccgaaaacgt ctcgtccgag caacgccgct
     8101 acgccaaaac catcaacttc ggcttaattt acggtatggg gcaatacggt ttggcaaaat
     8161 cattgggcat cgacaacctt tccgccaaaa actttatcga ccgctacttc gcccgctacc
     8221 ccggcgtcgc cgaatacatg cagcgcacca aagaacaagc cgccgcccaa ggctacgtcg
     8281 aaaccctgtt cggcagaagg ctctatttgc ccgacatccg caacaaaaac gccaacgccc
     8341 gcgcaggagc cgagcgcgcc gccatcaatg cccccatgca aggcaccgcg tccgacctca
     8401 tcaaacgcgc catgatagac gtgcgaaact gtctttcaga cggcatcggc agcaaactgg
     8461 tcatgcaggt gcatgacgaa ctggtgctgg aagtcgttga aaccgaactg gattttgtca
     8521 aagaaaaact gccgcagatt atggcgaaag tggacggcgg attattggat gtaccgctgg
     8581 tggctgaggt tggcgtaggg gagaattggg aagaggcaca ttgattgaaa ggtgttatat
     8641 gctatcttta tttaaataaa atttaatttt tggtatattt tttctaaatg ttcctat
//
LOCUS       DAXHRI010000077         8656 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXHRI010000077 DAXHRI010000000
VERSION     DAXHRI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.077"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="ACFM75_001299"
     CDS             complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="ACFM75_001299"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC6255838.1"
                     /translation="MKLSELFNPNEFAAQHLSFGDEAALWETLGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFDFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTVGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDTLSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHVVTDMHNASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVERGEWPKEDNPLVNAPHTASDVTGEWTHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3125..3484)
                     /locus_tag="ACFM75_001300"
     CDS             complement(3125..3484)
                     /locus_tag="ACFM75_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255839.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVLAGFMVLVSGFPLSLLLVVLLVLTCCILARCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3483..3662
                     /locus_tag="ACFM75_001301"
     CDS             3483..3662
                     /locus_tag="ACFM75_001301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC6255840.1"
                     /translation="MPSEPAGRLFQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3655..4296)
                     /locus_tag="ACFM75_001302"
     CDS             complement(3655..4296)
                     /locus_tag="ACFM75_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC6255841.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4286..4837)
                     /locus_tag="ACFM75_001303"
     CDS             complement(4286..4837)
                     /locus_tag="ACFM75_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC6255842.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4841..5359)
                     /locus_tag="ACFM75_001304"
     CDS             complement(4841..5359)
                     /locus_tag="ACFM75_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC6255843.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RRGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5359..7020)
                     /gene="pqiB"
                     /locus_tag="ACFM75_001305"
     CDS             complement(5359..7020)
                     /gene="pqiB"
                     /locus_tag="ACFM75_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC6255844.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGITLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRQTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7013..8347)
                     /locus_tag="ACFM75_001306"
     CDS             complement(7013..8347)
                     /locus_tag="ACFM75_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC6255845.1"
                     /translation="MPPLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     ADVPQLDRGEAAFCPRCGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1945 a         2219 c         2442 g         2050 t
ORIGIN      
        1 ttgtttgtcc gtgtggaaat gacatcaaag ggtgtgaata ggcttttcat ctttgaaatc
       61 tgtttgcacg atcggatgaa taaaaagtag gatgataaaa aagctgatgt atggatactg
      121 gtttaaccca tttgttttaa agttatagtc gagatacgtg tattggtgca accagtctgc
      181 cgtcgacgaa aatgccgtct gaaagtgttt cagacggcat ttgtcttcgg ggtttcaacg
      241 gtcagtcttc ataattttcc atcggtgggc agctgcacac gagattgcgg tcgccgtaca
      301 cgtcgtccac acggttgacg ctcggccaga atttgtgttc gcggacgaac ggcaatggga
      361 agacggcttc ttcgcgggaa tacggatgcg tccattcgcc agttacgtcg gatgcggtat
      421 gcggagcgtt caccaacggg ttgtcctctt tcggccattc gccgcgctcg actttcaaca
      481 cttcctgttt gatttgtttc agggcggcga tgaagcggtc gagttcggct ttgctctcgc
      541 tttcggtcgg ctcgatcatc agcgtgccgg caacggggaa ggagacggtc ggggcgtgga
      601 agccgtagtc catcaggcgt ttggcgatgt cggtttcggt aatgccgctt tcggctttga
      661 gcggacgcaa gtcgacgata cattcgtgcg cgacgcggcc gtttttgcct gtatacagaa
      721 tcggatagtc ttcgctcaag cgtttggcaa cgtagttggc gttgagcaat gcccagcgtg
      781 ttgcctgctc catgccttgt ttgcccatca tggtcaggta catccaagta atcggcagga
      841 tggacgcaga accgaaagct gcggcagcca cggcagtttg atcggcactg gcgttgtgca
      901 tatcggttac cacatggcct ggtgcgaacg gagccagatg ggctttcaag ccaatcggac
      961 ccatgcccgg accgccgccg ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca
     1021 acacgtccgc gccgacttcg gcaggctgca tgatgccgat ttgggcgttg aggttggcgc
     1081 cgtccatgta aacctgtccg ccgttttcat ggatgatgcg gcagatgtcg cggatgcctt
     1141 cttcgtacac gccgtgggta gacggatagg taatcatgat ggcggacaaa gtgtcgcggt
     1201 gttgctcggc tttggctttc aaatcgtcga tgttgacgtt gccgtgttcg tcggtatcga
     1261 caacgacgac tttcaagccc agcatggccg cagtcgcagg gttggtaccg tgggcggatt
     1321 tcggaatcag acagatgttg cggtgtccct cgccgttggc ttcttggtaa cggcggatgg
     1381 acagcatacc gctgtattcg ccctgcgcgc cggagttggg ctggaaggaa atcgcgtcaa
     1441 agccggtaat ggctttcagg ctgttttcca tatcggcaag caattcgcgg tagccgacgg
     1501 tttgcgcttc gggagcgtaa gggtggatgt cggagaactc cgcccaagta atcggcagca
     1561 tttccgcagt cgcgttgagt ttcatggtgc agctaccgag cgaaatcatg ctgcggttca
     1621 tcgccaaatc gcggtcttcg agttttttca gatagcgcaa catttcgtgt tcggtgtggt
     1681 aacggttgaa cacaggatgt tgcagaatgt catcttgacg cagcaattcg gcgttcagac
     1741 ggcctttgac atcatcggca aatgcgaacg catccttgcc ggtaaacgcg cggtacaaat
     1801 caaccaaatc ttcgcatgcc gatgtttcgt ggaatgcagc cgcaacttga gtatcgttaa
     1861 cgcggcgcag gttatagccc gattccaaag cagcggcaaa cacttggtct gctttctctt
     1921 tgttgccgaa atcgacggta acggtatcga agaagacctt atgaaccaca ttcaggccgt
     1981 ctgaaaccag cgcatcggca aaggcagaag ccagcgcgtg aatgcggttg gcgatgcgtt
     2041 tcacaccttc agggccgtgg taaacggcgt acatacccgc caagttcgcc agcaatgcct
     2101 gcgcggtaca aatattggat gtcgcttttt cgcggcggat gtgttgctcg cgggtggaca
     2161 acgccatgcg caaggcaggt ttgcccgatg cgtctttgga tacgccgatg atacggcccg
     2221 gggcggaacg tttgaactcg tctttaaacg cgaaataagc agcatgcgga ccgccgaagc
     2281 ccatcggtac gccgaagcgt tgggtgttgc ccaaagcaat atccgcgccc aattcggcag
     2341 gcgatttcag caaaaccaag ctcataatgt cggcggaaac ggccacgatt gtgcctttgg
     2401 ctttcagacg gccgataacg tcctgcaagt cttgcacatc gccgtctttg ccgacgtatt
     2461 ggaacagcgc gccgaagtat tcgccctcgt cggcttgagc aaaatcgccg accaccagtt
     2521 caaagtcgaa atacttggcg cgggttttca tcacgtccaa agtttgcgga tacacgcgcg
     2581 catccacaaa gaaacgctcg gatttcacct tgcccacgcg gtgcgccatc gccatcgctt
     2641 cagccgcagc agttgcctcg tccagcaaag acgcgcccgc cacagggaaa ccggtcaaat
     2701 cgatacacac ttgttggaag tttaacaacg cttccaaacg accctgggca atttccgcct
     2761 gatacggcgt gtaggcggtg taccaacccg gattttccaa cacgttgcgc aaaatcacgt
     2821 tcggcacgcg ggtcgggtaa tagcctaaac cgatatagga cttgttgatt acgtttttcg
     2881 acgcaatgcc tttcaatttc gccaatgcgt ccgcctcggt caaggcctcg ggcaaatcga
     2941 gttcggacgg catacggatg ctttgcggca cggtattgcc gacaaaatcg tccatactct
     3001 tctcgccgag cgtttcccaa agcgcggcct cgtcgccgaa actcaaatgt tgcgcggcaa
     3061 attcgttggg gttgaacagt tcggacagtt tcatttctac tccttcgggt ttgtagggct
     3121 gccatcaggc agaattttta gacatcggat gattcgggca agggtagcac aaaagacggg
     3181 ataatggcgg acaacgcgcc agaatgcagc aggtcaggac aagcagcacc acaagcagca
     3241 gcgataaggg aaagccgctg accaaaacca tgaaacctgc aagcaccagc caagccaatg
     3301 gatacgccgg aagcggaaaa acaatattga gcagaaccaa aatcacgaca aacagcggca
     3361 gacccgccat caggttggca cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt
     3421 cgcaatattc catgatttca gcctgtccgg gctttatcca cagcaggctc ggacggtcgg
     3481 tcatgccgtc tgaaccggca ggccgtcttt ttcaaaaaac gccataccgc tgcaacttca
     3541 tgcggctgta tggcgtaggc tttgcacttc agacggcata tgcgtcccga agcagtttgg
     3601 tgtcccagtc ggttcatgat tatataaaca acttgggtgc ggataaacct ttgtttatct
     3661 aaacccctgt tttctcaaaa attgattcaa atcttcggca aatttccgat agcctttgcc
     3721 gttggcgtgg atttggtcgg atttcagatt attatcgccc aaaatttccg cccacgcgcc
     3781 accgaacagc ggaatgccgt attcctcgga caaatcctca tacagcggat gatcgctcaa
     3841 atgcccgaac aacgcgccca gtgtgatgtg cggcacgccg acgaggacgg cggggatgtt
     3901 ttccttctgc acggtttcga tgattttcgc gatattggcg cgggtctgct ccttgggaac
     3961 tttgcgcaga aagtcgttgc cgcctatgcc gacaatcaca agcttgggtt tgcgtgccaa
     4021 cagcgcgggc aggcgcgaca gggcttgggc ggatgtatcg cccgatacgc cgccgttgac
     4081 aatattccaa cccgtcagtt tttgcagttg cgcggggtag gattcgccgg ggtttgcgcc
     4141 gtagccgaag gtaagcgaat cgcccaaggc aagcacggtg cttccttcgg gaattttggc
     4201 gtgggttcgg gcggattttc tgccgcaggc ggtaaggagc aacgcgcctg cgccgaggag
     4261 gaaggttctt ctgttcattg gtttttcaga tggcatcgag ccatttcggg tggtatttca
     4321 gggtttcacg gtaaaccgga cagctttcgg cgtgcgcgcc tttgaggtag ccggtactca
     4381 tcagaaattc gccgacgatt tcgccgccga cgaatttgaa atgtttttta aagagtttaa
     4441 cccattcgtc tttgcttcgc ggatggtgcg cgtcgagcca gttcttgaac gagccgtatt
     4501 cttgttgcaa cgcttggatt tgccgtgcat tgaaaatggc ggcatcgatt ttcaggcggt
     4561 tgcggacgat gccagcgtcg gcaagcagcc gctcaatatc ggcttcgcca aatgcggcaa
     4621 ccgtatcgat gtcgaaacct tcaaatgccg tctgaaacgc ctgccgcttc ttcagcatca
     4681 gcgtccagtt taatcctgcc tgattgattt ccaacaccag ccgctcaaac aattcattgt
     4741 cgtcctcaat cggaaaaccg tattgcgtgt cgtggtaatg tttgttcggg ttgtcggtgt
     4801 tttcgggaag tgaggcggca aattcgcaat agttcacggt ttactcgacc atctgttgcg
     4861 ccgcctgttt cagtccctgt tcgagtgcgg cggtcatcgc ggcgtagccg tcaccctgct
     4921 gttcggtttc gatatggaag ggtctgttcg taccgtcggg taggacggcg tagccgctga
     4981 tgagggtttt gcccgtgtag ctgccttgga atgcgtcaat atagaccgtc catttttcgg
     5041 tactgcccct gcgtgaggca ggaacaaaga tgcgtgtgct gtccaaacgg ttgaatgcat
     5101 tgctcaacgc cgcttcgagc atatcgtcca aggtgtctgc ccaaacgtgg ttttgtgcgg
     5161 tgttgaggcg gtaggggtcg gtttgataga ccagtccgcc gcgtttgagc ggttcggcaa
     5221 ggcggacttc gacggcggtt tcgccgcctt gcgttgcagg gcggatgtag cggctgtcgg
     5281 gcaacacgaa atattgtgtg ctttgcacag taccgcaggc ggcaagcgtc agggcggcgg
     5341 cgatggggaa gaggcgcatt atcggcttcc tttcgggatg gggtctttgc tgctgctgtt
     5401 gaaaatcagc gcgttgggtt tttctttcaa agtattaatt acgggttgaa cgtcttttaa
     5461 ggttttgtcc aaactttgca acgtattttg tacgtcgccg tagataggcg attgaggcga
     5521 tacgccttgc agggtttgac gtaattcttt cagggtttgg ttcagttcgt tcggaatgtt
     5581 ttgtgtctgc ggtttgccga ccagtttgtc gatggagctt agggcggcat tggcagattt
     5641 gagtgtggat ttgagctcgg caagcgaacc gttcaattcg gcaaccgtct tatctaaagg
     5701 cagtttgtcg aacttgtcca gcaaatccgc caatttgacc tgcaaatcgt ccaaaccgcc
     5761 gccctgggtc gcgataacgg tatcgcctgc ataaacggta tgcggtcgca gcttaggcga
     5821 tgcggaaggc tgatcgttca actcaatcat tttgcttccg gtcagcaggt tgttgctgga
     5881 gatggtggcg gtcaggcctt tgtttaaggc cgtctgaaat tgttgtttcc aatgttcttt
     5941 gctttgttcg tcggcattga tttccaaacg ggaaggttca atgcggatgc gtacgggtat
     6001 ccagccgttt tcaaacaggt gcaggctgtc gttgcggtcg aaataaggga catcggaaac
     6061 cacgccgaca ttcagccctt tgtactcgac gggcgaaccg acggtcaggc cgcgcacgga
     6121 ttgtttgaaa aacgcggtgt agtacagcga gcggtcgtca ggcaggttgg cgacttcgct
     6181 gcggctgtcg taaagcgtga agctgtcttc gcttttgacg tttttactgt ttttggtttt
     6241 cggcgaatca aatgaaatcg cgcccgacag cagggcaggc agaggggcgg aattgagtgt
     6301 gatgccgctg cctgtggttt cgatattgat gccgctttcc agccaaaaac ggctggcgga
     6361 atgaatcagt ttgtcgttgg ggctttggat gaagatggtg taatgcacgc tttggtcgga
     6421 cgggtcgaaa tgcgcgcttt cgacttgccc gaccataaag ttttcataca aaacagggct
     6481 gttgacgttg aggatgcggt cgtttttacc aatcaaattc aagcgcagcc cgctttgccc
     6541 gatggcggta acgggcggaa tgtcctgcac ttggaacacg tcttttgcct cgtcgctttt
     6601 gccgggtgta aaggcgatgt acgaacccga aagcagcgta cccaaaccgg ttacgccgct
     6661 ttggtcgata cgcggcttga ccacccaaaa ctgggtatcg ctgcggatga ggccggatac
     6721 gtccgcattg agttgggcgg ttacttccac gcctttttgg tcgtcgcgca gtttgattcg
     6781 ggtaacgcgt ccgacatcga tgctcaatac tttgatgacc gtattgttga cctcaatgcc
     6841 ttccgcgctg tccatcaaga gcgtaaccac aggtcccctg ttgcggattt ccttaaccca
     6901 aagccagccg ccggcaatca gcgcgatcag cggaaccagc cagacggcag agaggaaggt
     6961 gttgtttttg cggacgcgtg cttgggcgtg tccgtttgga ggagggctgt tgtcagtcat
     7021 gtttttccgt ttcattgaaa gcaatgccgt ctgaagcgcg tttgtcccaa agcaggcgcg
     7081 ggtcgaaata ataggcggac agcatcgtca gaatcacgac caggcagaaa tagactgccg
     7141 cactgcccgg aatgacgcgc gcggcataag tgtggaacga acacatcaaa ataataatca
     7201 caaaaatatc aatcatcgac cagcggccga ccgcttcggt gatgcggtag aggtgcgaca
     7261 atttctttgc gcccgttggc aaagcgaagc gggcggacgc aatcaaaacc gacattgccg
     7321 caatcttcag taccggcacc aaaatactcg cgctgaaaat aaccgccgca atcagcctgt
     7381 cgccctcgtc ccacatataa gcgatgccgt taaggatggt attgacctcc gtggcggcag
     7441 gattggacga aatcataatc ggcaggatat tggcagggaa atacaaaata accgccgccg
     7501 tcagaaacgc cgacgaaata ctcagacttt tcggccgccg gcggtacagt tccgcaccgc
     7561 acacgccgca gggggattcg gcactgtcgc ggaaatacag gcagcggctg caacaggttt
     7621 tgccttctga tgccgtctga accgcattat cccccgtcag ccgcccgatt tgaaaataca
     7681 cccaatgctg gggaacggat accgaagtcc gaatcagcat aaccgacagc gcgaacatca
     7741 gataaaacgc cgacccgaag cgaacctctg ccacagacga gagcttgata tacgccacca
     7801 gagtggaaac aaaaaacaca tccaccatca tcgcctgcct caagcgcacc attacgcgtg
     7861 ttgccaaacg cagcgcagga tacgcctgtt tccgtatcag cgcggcatag acatacaggc
     7921 acagcagcag aaacagaacc ggcgcgccga aggtcagcac aaacatcact tcggctaaaa
     7981 aaccataatc ctgaaacacc atcaggcgca tcatctcggg cagcgaaagg acggatgccg
     8041 cacccggtat cccgacctcg atatacgtca taccgtaagc aaacgccatc aaaatcagcg
     8101 aagccgccgc ataagcgggc ggggcggaaa aaggatgcct gcccaccctg aagagcttat
     8161 agccgcagcg cgggcagaac gccgcctccc ccctgtccaa ttgcggcaca tccgcgcggc
     8221 agccgcattc cgggcaatct accgtatgcg gcggcagggc ttcgtcgcgg cagttgccgc
     8281 gaaggctgtg ggggatattt tcggcaaacg gcttcatcgg gaaagtggca aatataagtg
     8341 gcggcattat aaaggaaaaa aggcgataca cccaatcagg atgcaaaacc ggcaggttcg
     8401 gcagtatgaa caaacccttg gtgtccggca aaaatatttt ttgagcggtt gtccgccgac
     8461 tgccgaaccc atttgataaa atcccgattc tgtaaagaat agtaaaatac ctgcttaaaa
     8521 acatcacgca aatgatattt tttccaaaaa tatgtgataa taaatttcgt ttatgattct
     8581 ttattatttt tatagtggat taacaaaaat caggacaagg cggcgaagcc gcagacagta
     8641 caaatagtac ggaacc
//
LOCUS       DAXHRI010000078         8605 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXHRI010000078 DAXHRI010000000
VERSION     DAXHRI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.078"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            86..301
                     /locus_tag="ACFM75_001307"
     CDS             86..301
                     /locus_tag="ACFM75_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255846.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(491..1030)
                     /locus_tag="ACFM75_001308"
                     /pseudo
     CDS             complement(491..1030)
                     /locus_tag="ACFM75_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
     gene            complement(1387..3594)
                     /gene="uvrD"
                     /locus_tag="ACFM75_001309"
     CDS             complement(1387..3594)
                     /gene="uvrD"
                     /locus_tag="ACFM75_001309"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC6255847.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTRRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQLSLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNATLESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            3778..4425
                     /gene="pxpB"
                     /locus_tag="ACFM75_001310"
     CDS             3778..4425
                     /gene="pxpB"
                     /locus_tag="ACFM75_001310"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC6255848.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4422..5351
                     /locus_tag="ACFM75_001311"
     CDS             4422..5351
                     /locus_tag="ACFM75_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC6255849.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRSYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            5338..6075
                     /gene="pxpA"
                     /locus_tag="ACFM75_001312"
     CDS             5338..6075
                     /gene="pxpA"
                     /locus_tag="ACFM75_001312"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC6255850.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            6204..7397
                     /locus_tag="ACFM75_001313"
     CDS             6204..7397
                     /locus_tag="ACFM75_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6255851.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            7505..8197
                     /locus_tag="ACFM75_001314"
     CDS             7505..8197
                     /locus_tag="ACFM75_001314"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6255852.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            8463..>8605
                     /locus_tag="ACFM75_001315"
     CDS             8463..>8605
                     /locus_tag="ACFM75_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6255853.1"
                     /translation="MSYTQLTQGERYHIQYLSRHCTVTEIAKQLNRHKSTISREIRRH
                     RTQG"
BASE COUNT         1909 a         2203 c         2413 g         2080 t
ORIGIN      
        1 agagacccaa attcaaaaga tggtggaact gcatttagac caaccttagg caaaacttac
       61 tttgacaaac aaaaataaaa ttattatgaa tataatcaat tacacaggtg atgacattat
      121 tatttcttta acaagagaag agcttcaatt attgcgttcc cttgttattg aaatttatgc
      181 aggtgtttgc atagatgcag aagaatttga gattgtgtca ggaattcgca attcaaaatt
      241 ggtacaagag ctagaacaac aattaattga agcatatgat ttaatggata caaaaggata
      301 accgaattat atttgagata gctccaaact tgataagttc tgttaataat gtcaatgctg
      361 tcggagacag tttttctttg caaaggtctt ggatggcgcg ttccaccaaa ccgttttcct
      421 tacggattca agcgattatc cgttatttcg cccgttttcc cttgatgatt ccggtttttc
      481 aaccgccgtt ttatatttcc tcgtcaataa actcatcttc atgccgcaag ctgtgaataa
      541 aggtttcaaa atcgggggca agttcgacga tttaaaaatc cgattcttgt tcgacaaaca
      601 ccactttcgg ctctccgtct cttccacact cccgatagtc taaggcaaat agggcatgac
      661 cgcctgacgg gtcgttggca aaatacacac cgatgggcgg atactcccat tcttccagcc
      721 aaagtttttg ccctgtttcg ccgcacaggc ttccttcttt ttcaaaaccg attcccgata
      781 cctcgcaaat ttgcacatga tctttcgccc acgaatttct ctgtgtggtc ggaaaacagt
      841 ttttgacaaa tattccgccg ttttgtaccg ccatcaattc aataaaactt tgcggcaatt
      901 tatagcccag ttcactttct acggcagcca aaatttcagg ggtaaacggg gcttctttgt
      961 agttttcatc tgcccaactg tcggattccc atacggagga caaatcaaaa tctttaaaaa
     1021 cttgggacat aatgttcttc cttattgatt ttcagctata aacaagggtt aggtatctat
     1081 acccggcttt taccgtccgt cattcccgcg caggcgggaa tctagaacgt ggaatctaaa
     1141 gaaacctttt atccgataag ttcccgtgcc gaaaggtctg gattcccgcc tgcgcgggaa
     1201 tgacggcggc ggttgcggca aaatttgttg ttaccggatt cagacggcct tttgccttgt
     1261 ccttgctaca gttttttgcc ataggtcgga ttctcgaatc cgacattttc caacagcggt
     1321 ttttcggaaa cgatagacgc gtcaaatatt tttgtcggat acgagtatcc agcctgcatt
     1381 tcaaatttac atctcttcca atttcgcaaa cttggtgtcc aactctttca cgccctgttt
     1441 gccgaaattg atggtcagtc gggcggattc gcctttatct gcggcatcga taatcacgcc
     1501 ggtgccaaat ttggcgtggc ggacgttttg tccgatacgg aaacctgcgt aggtttgggg
     1561 ctgtttgtag tcgtcgatga ttttgtcttt ggatgcggcg gtttggcgcg tgttgccgta
     1621 actgtcgtag gcaggctttt tgacggacag gtagtgcaat acttcgggtg ggatctcttc
     1681 gacgaagcgg gagacgatgc cgaattgggt ttgtccgtgc agcatgcgtt gttgcgccat
     1741 ggtgatgtag aggcgtttgc gggcgcgggt gatggcgacg tacatgaggc ggcgttcttc
     1801 ttcgaggccg ccgcgttcgg caaggctcat ttcgctgggg aagcggcctt cttccatgcc
     1861 ggtgaggaag acggcgttaa attccaagcc tttggcggcg tggacggtca tgagttggac
     1921 ggctttttcg cctgcacctg cttgattttc gccggattcg agggtggcgt tgctcaagaa
     1981 agcgaggatg gggaaggcgg gatcgtctga aatgttttca ggcaggattt cgaagttgct
     2041 gtcttcgggt ttgaactcga tggcggcgtt gacgagttcg tcaaggttgt cgagacggtc
     2101 ttggttgtcg cctttttggg tgcggtagtg ttcggtcaag ccactgtctt tgaggatgcc
     2161 gacgatgatt tcggacaggg acagttgtcc gacttggttg cgcagggctt caatcaggcg
     2221 gacgaaggcg acgactttgg cggctttcgc gccggcgttg caggcggctt gccagagggt
     2281 gatgccttgt tcgtttgagg ccgtctgaag attttcgacg gtacgtgcac cgatgccgcg
     2341 cggtgggaag ttgatgacgc gcaaaagggc gttgtcgtcg tcgggattga cggcgaggcg
     2401 taggtaggcg agcgcgtgtt tgatttcttg gcgttcgtaa aagcgcaagc cgccgtagat
     2461 tttgtagggg atgccgctgc ggaacaggct ttgttcgatg acgcgcgatt gggcgttgct
     2521 gcggtagagg acggcgattt cgtccaaatc ccagccttcg cgttcgaggg ctttggtttc
     2581 gtccacgatg aaccgggctt cttcgaggtc ggtaaaggcg gagtagtagc ggattttgtc
     2641 gcctgcttcg gcgtcggtgc gcaggttttt gccgaggcgt tcgtcgttgt tttcgataac
     2701 ggcgttggcg gcggcgagga tgttgccgac ggagcggtag ttttgttcga gtttgacggg
     2761 cgcgtcgatg tggaattctt ccatcagcgc ggtcatattg ccaacgtttg cgccacggaa
     2821 acggtagatg ctttggtcgt cgtcgccgac ggcaaatacc gccgcgttgc cgcccgccat
     2881 gagtttgagc caggcgtatt gcagtttgtt ggtgtcttgg aattcgtcga ccagaatgtg
     2941 attgaagcgg ttttggtagt gttggcgcag gatttcgttg ctttgcagca tttcgtagct
     3001 gcggagcata agctcggcga aatcgaccac gccttcgcgt tggcagattt tgtcgtattc
     3061 ggcgtagcac tcaatcatgc ggcgcgtgtg cgggtcgggc gcgctcaaca cggaagcgcg
     3121 caaaccggat tctttttgcg cgttgataaa gccttgcagc gaacgcggcg cgatgatttc
     3181 ttcggcgata ttgaggcttt tgagcagacg tttgatcagg gaaagttggt cgccgctgtc
     3241 gaggatttga aaggatgacg gcaaaccggc atcgcggtgg tgcagccgta gaaagcggtg
     3301 gcagaggccg tggaacgtgc cgagccacat agcgcggacg tcgacgggaa tcatcgcgcc
     3361 gagtcgggtt tgcatttctt tggcggcttt gttggtaaac gttaccgcca taatgctgtg
     3421 cacgcttgcc tgaccggttt gcaaaagcca tgcgatacgc gtggtcagca cgcgcgtttt
     3481 gccgctgccc gcgcctgcca gcacaagggc ggattgcggc ggccaggtta cggcggaaag
     3541 ttgttcaggg ttcaagcctt gcagcaggtt gggggcggat tggtcgggaa acatagagat
     3601 gccgtctgaa aagtggaatg cgctatttta atagaaacgg tttaaggtcg tctgaaaaag
     3661 tgcagcggca gggcagcact tttccaatcg acggtttgat gatgcagcac agaagatatt
     3721 gacaaaccgc cgcccctgca tatagattca ttaaaacata accaaaatga acaacaaatg
     3781 agaatagaga tcacaccaat cagcgaatcc gctttggtct gccgactgaa tgcaccttcc
     3841 gaactgggca aacagcaaaa gttgtgggcg tttgccgctg cgctcgggca gcacgacagg
     3901 attgaggaag tggtggtcgg catgaacaat ctgaccgtgt tcacccgttt cgataccgat
     3961 ttggcgacgc ttgccgatga attgcaatat gtgtgggaac acaccgccgt tacagaccat
     4021 cagggcaaac tggtggaaat tcccgtctgc tacggcggcg aatacggccc ggatttggcg
     4081 gaagtcgctg ctttccatca gacggttatt tccgaaatcg tccgccgcca tacggcgcaa
     4141 acttataccg tatttatgat gggcttccag cctggtttcc cttatctggg cggcttgccc
     4201 gaagcattgc acacgccccg ccgtgccgtg ccgagaacgt ccgttcctgc cggttcggtc
     4261 ggtatcggcg gcagtcagac cggtgtgtat ccgttcgctt cgcccggcgg ctggcagatt
     4321 atcggcagaa ccgaattacc cttgttccga gccgatttga atccgccgac cctgctggcg
     4381 gcgggtgacc aagtccgctt tgttgcagaa aggattgagc catgattcac gtttcggcag
     4441 tgcaggcacc ggcgcatatt caggataccg gacgctacgg acaccggagt tacggcatcg
     4501 gtcatgccgg tgcgatggac acggttgctt tggcggcggg taatatttta ttgggcaacg
     4561 acgaaggcac ggccgcaatc gaaatcgctt tgggcgggat aatgctggtg tttgaacggg
     4621 atacgccgtt ttgtctcacc ggtgccgtgt atcaggcgga attggacggc gaaccggtct
     4681 attcgtattg gcgttatacc gcccgcaaag ggcagacctt gaaaatggtg cgtgccgtgc
     4741 agggcatgta cggctatgtg tgcgtggcgg gcggatttga tgtgccggaa gtgatgggtt
     4801 cgagaagcac cgacctgaaa gccggtttcg gcggccatca gggcagaatg ctgcaaaaag
     4861 gtgattgtct ccccatcggc aaaggtgcgc aggaattgtc caaagtcggc attgccccga
     4921 taccgtttac cgataccgtc caccttgttc cttcgtcgga atatgccgct ttcagtgaaa
     4981 aagggcgtct gaatctggaa cgggaaacgt ggacgctgca aagcgatagc aaccgcatgg
     5041 gctaccgctt cgacggacag ccgctgaccc tgtcgcaacc tttggaaatg ctgtcccacg
     5101 ctgttcaggc aggaaccgtg caggtgccgc ccggcggcaa accgattatc ctgctggccg
     5161 atgcgcaaac caccggcggt tatccgaaaa tcgctaccgt tgccgccgcc gatttgggca
     5221 ggctggcaca ggtgcgcttc ggcagcaaag tcaaattcaa aataatcggc ttgaaagaag
     5281 ccaccgccct gcggcgcaaa aaccaagtct atctgaacca aatacggaga atcacccatg
     5341 aagcaggttg atttaaacgc cgatctcgcc gaaggctgcg gcagcgacga agccttgttg
     5401 cagctgatta cttcggccaa catcgcctgc gcccaacacg ccggcagcat tgccgatatt
     5461 cgggcggcat tggcgtatgc ccaacaaaac ggcgtgcgca tcggagcaca ccccggctat
     5521 cccgatcggg aaaacttcgg ccgtaccgaa atgaatctgt ccgaagccga tttgcgggcg
     5581 tgtctgaatt accagttggg cgcattgcag gccttgtgcc gtgatcaggg tttggaaatg
     5641 gcttatgtca aaccgcacgg cgcaatgtac aatcaagcgg cgaaaaaccg tgcgctggcg
     5701 gataccgttg cccgaattgt ggcggatttc gacccgaaat tgaaattgat ggcactttcc
     5761 ggcagcctgc tcttggaagc cggaaaagcc gcaggcttgg gtgtgatttc cgaagtattc
     5821 gccgaccgcc gctatatgcc cgacggtacg ctggttcccc gcagccgccc cgatgcgcag
     5881 gtggacagcg acgaagaagc catcgcccaa gtattgcaga tggtgcggga cgggcaggtc
     5941 aaagcagtgg acggcagcct ggttgccgtg caagccgaca gcatctgtct gcacggagac
     6001 gggccgcacg ccgtggtgtt tgccgaaaaa atccggcagg aactactggc ggcaggcatc
     6061 aaagtttctg cttgaaaaac agttcatagg ccgtctgaaa accgcaggtt tcgccaaaac
     6121 gagcaaagcg agtttctgcg tagctaaaac cgcaggtttc gcccaaacac agcaacaacc
     6181 aattttatag aggataagcg attatgtctg atcaaaaaaa ccgcagaaat gccttaatcg
     6241 gcgctgcatt cctgatggcg acttccgcca tcggcccggg ctttctgacc caaaccgcca
     6301 ccttcaccca agcactggcg gcaagtttcg gctttgtgat tctgctctcg attctgctcg
     6361 acatcggggc gcagctcaat atttggcgga ttgtcgccgt ttccgaaaaa caggcgcagg
     6421 atattgccaa tcaggtcttg cccggcgcag gctatttctt ggctgtgctg attgtgatgg
     6481 gcggtttggc gttcaatatt ggcaacgtcg gcggcgcagg cttgggtctg aacctgctga
     6541 ccggactgtc gccggaaacc ggtgccgtga tcagcggcgt gattgccatc ggtgtgtttc
     6601 tgtttaaaga agcaggcaaa gtgatggaca aattcgccca agtgatgggt ttcgtaatga
     6661 ttgcgctgac ggtttatgtg gcatggcagg cgaatccgcc gctggcagat gccgccgtgc
     6721 atacctttat gccggaaaaa ctcgatgcaa tggcgattgt tacactggtg ggcggcacgg
     6781 tcggcggcta catcaccttc gccggtgcgc accgtctgct ggacgcaggt atcaaaggca
     6841 aatcggcgtt gccggaagtg agccaaagct cggtgcgggc gatcctgatt gcctcgatta
     6901 tgcggattgt attgtttttg gcggttttgg gcgtggtcag ccaaggcgta cagctcaatc
     6961 ccgacaaccc tgcttccaca ccgtttgaat atgcggcggg atacatcggc ctgctgattt
     7021 tcggcgtggt gatttgggcg gcttcgatta cttcggtgat tggtgcggct tatacttcgg
     7081 tgtcgttctt ctccggtctc agcccgtcta tcgaacgcaa taaaaacaaa tggattattg
     7141 cctttatcgc cgtgtccacc gccgtatttt ccaccatcgg caaaccggcg caggtgctgg
     7201 tgttcgtagg cgcattaaac ggcctgattt taccgatttc cctcggtctg attctgattg
     7261 ccgcctacaa aaccaaaatt gtcggcgact acaaacaccc gctgtggctc accgtttccg
     7321 gcgtgattgt ggtcggtttg atggcagtac tcagcgccat caccatcagc aaatatatcg
     7381 gcggcttgtt cggctgaacc ttgtagcgca gatgtttgat tttgtatatt atccgaagtt
     7441 tgccgcctga tatggaaaat tcctgtttca gacgatgtat ccggtctgaa acctattcac
     7501 aactatgaaa cgcttcacct atactctttc cgacggcttg tgcatcgaaa tcgaactcaa
     7561 acgcagtgcc aagaaaaatc tgattctgcg ccccgtcaat atgcagacgg tcagcatcaa
     7621 cgtcccaccc ttttttcaag accacgcgtt agcaaactgg ctggcggcaa acgaaacgat
     7681 tttgcggaac acgcttgcca aaacgcccgt gcatcctgtt tcccacccaa acttacccga
     7741 gtggatttgg tatcggggaa taaagaccaa gctggatacc cacagccaaa gccatatccg
     7801 tatcacgtcg tctgaaatcc tgcttccccg aaaagaaacc gccgcacaaa tcgaccacct
     7861 gcgccgcctg ttgaacgaac gcgcccgcga atacctgctg ccccgccttg aaaaacacgc
     7921 agccgaaaca ggactgacgc ccaccgccac agacctgagc aacgccaaaa ccttttgggg
     7981 cgtatgccgc ccgcacaccg gcatccgcct caactggcgg ctgatcggca cgcccgaata
     8041 cgtcgccgac tatgtctgca tccacgaact ctgccacctc cgccaccccg accacagtcc
     8101 gcgcttttgg catttggtga acacgctgac gccgcatacc gacaatgcta aaagttggct
     8161 gaaggcgcac gggcgggaat tgtttgtgct ggggtaaagg ctaaccgtag cgtgggcttc
     8221 gcccgcgaga atccaccccc tcccgtacgg agggagaggg ttggagagag ggtggctctt
     8281 gtcccttgtt ctgtatattt gcaagccacc ctatcctagc cttccccccg ttggataagg
     8341 gaaagggatc aagctgctgt aactgaaaga ggtcggcgga ttcgcatttg aagtgcaact
     8401 ttccctaaca gaaaaaggcc agtatgcggt agcatacgac ctttcctgca agaaagattg
     8461 ccatgagcta cacgcaactg acccagggcg aacgatacca catccaatac ctgtcccgcc
     8521 actgcaccgt caccgaaatc gccaaacagc tgaaccgcca caaaagcacc atcagccgcg
     8581 aaatcagacg gcaccgcacc caagg
//
LOCUS       DAXHRI010000079         8561 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXHRI010000079 DAXHRI010000000
VERSION     DAXHRI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.079"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(75..593)
                     /locus_tag="ACFM75_001316"
     CDS             complement(75..593)
                     /locus_tag="ACFM75_001316"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC6255854.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPHAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(613..1998)
                     /locus_tag="ACFM75_001317"
     CDS             complement(613..1998)
                     /locus_tag="ACFM75_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC6255855.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVTAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2236..3777
                     /locus_tag="ACFM75_001318"
     CDS             2236..3777
                     /locus_tag="ACFM75_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6255856.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRCAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     RTETL"
     gene            3806..4405
                     /locus_tag="ACFM75_001319"
     CDS             3806..4405
                     /locus_tag="ACFM75_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC6255857.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4402..5007
                     /gene="nadD"
                     /locus_tag="ACFM75_001320"
     CDS             4402..5007
                     /gene="nadD"
                     /locus_tag="ACFM75_001320"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC6255858.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLASQGVSDGIPPAAARYIREHGLYEK"
     gene            5066..5452
                     /gene="rsfS"
                     /locus_tag="ACFM75_001321"
     CDS             5066..5452
                     /gene="rsfS"
                     /locus_tag="ACFM75_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC6255859.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5506..5976
                     /gene="rlmH"
                     /locus_tag="ACFM75_001322"
     CDS             5506..5976
                     /gene="rlmH"
                     /locus_tag="ACFM75_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC6255860.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            6051..6317
                     /locus_tag="ACFM75_001323"
     CDS             6051..6317
                     /locus_tag="ACFM75_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC6255861.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWMAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6474..7157
                     /locus_tag="ACFM75_001324"
     CDS             6474..7157
                     /locus_tag="ACFM75_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC6255862.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMIFTFGMGVLIGPVLQY
                     ALHIADGTKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7281..7793
                     /gene="coaD"
                     /locus_tag="ACFM75_001325"
     CDS             7281..7793
                     /gene="coaD"
                     /locus_tag="ACFM75_001325"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC6255863.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWMETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7825..8478
                     /locus_tag="ACFM75_001326"
     CDS             7825..8478
                     /locus_tag="ACFM75_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC6255864.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1866 a         2267 c         2502 g         1926 t
ORIGIN      
        1 tgtaatccat tctgttcccg atgtttatgc cgtctgaaac ccatcctgtg tcgggcttca
       61 gacggcatat tgcttcaaag caggttttcc gaggcaaccc agttcagaat atcggcttcg
      121 accgcttcgg gcgtgccggg gttggcgatt ttgaccccgt attcgccttc gcccagttcc
      181 tcgaggattt ccagttgcga atcgagcatc cctgctttca tgtaatgtcc tttgcgcgac
      241 atcatgcgct cgaggttgat gtcttgcggc ggactgaggt ggatgaaggc agctttgcct
      301 tcggctccgc gcagaatgtc gcggtagccg cgtttgaggg cggaacaggt tacgatggtg
      361 tggttcgcac cgttttgcgc ctgttgcgtc atccagtcgc gcagattgcc caaccacgga
      421 tagcggtctt catcggtcag cggaataccc gcgcccatct tgtcgcggtt ggcttgggtg
      481 tggaactcgt cgccttcggc atggggacat tgaccgaggt gtttctgcag ggacagcgcg
      541 gcggtggtct tgccgcagcc gcatacgccc ataacgacaa aatgcgtagt catttactat
      601 cctttccgtc tgtcagacga tggcgaacag cagtgcggac aaggcaaagc cgatgagtgc
      661 gatgagggtt tggttgaccg tccaggtttt cagcgtggtc ggtacgtcca tgtccaagag
      721 gcggccgacc agccagaagc cggagtcgtt gaagtggctg caaccgaccg aacctgccgc
      781 cgttgccaat acgatacagg cgagctgcca gtcggtaaag ccggcggcgg taacggcagg
      841 agccatcagc gcggcggcgg tggtcagggc gacggttgcc gaaccttgcg cgatacgcag
      901 tgccaaggcg acaaggaaac agcccaaaag gacgggaatg cccaaatccg ccatgctgtc
      961 ggcgagtgcc ttgccgatgc cggaagcgcg caaaacgccg ccgaacatac cgcccgcgcc
     1021 ggtaatcaga atcacggaac agacgggggc gagtgcgccg tccacggttt tttccaacgc
     1081 gctgccgctt tcgccgcgtt tgcgtcccaa gacaaacagt gcgaccaata cggaaatcag
     1141 aagggcgatc ggtgtcgaac cgattatttt tgccgtctga acccaggttt cgtccgcact
     1201 tacgagtttt tcgctgatga gggccgatac gccggtattc aggaaaatca gcagcatggg
     1261 aatcagcatg atggcgacga ccgttcctgc tttggcaggt tctttcggca ggtcgttgtc
     1321 ttgcgtgccg ccgctgagca gttcgggaac gggaacatgg atggtgcgcc ccaacacttt
     1381 gccgagcata tagccgctga aataccatgt gatgaaggcg gtcggcagac ccaaaatcaa
     1441 aacttggccg atgttcgcgc cgtaaaattc ggaagcggca atcgggcccg gatggggcgg
     1501 caggaagacg tgcatgacgg aaaatgcgcc gatggaggca agcgcgaagg gcagtacgtc
     1561 ctgtttcatg cgccgtgcgg tggcgaacac gatgggcagc atgacgatta gtccggcatc
     1621 gaagaaaatc gggaagccga aaatcagcga ggcaacgccc agcgcgaacg gtgcgcgttt
     1681 ttcgccgaac atccggatca gcgcgtccgc cagcgactgt gcgccgccgg atgtttcgac
     1741 cagacgtccg agcatcgcgc ccaggccgac cagaagcgcc acgccgccga gcgtgccgcc
     1801 gaagtttttg accagtacgt cgttgacaat gctgcctgtg ggcaaaccgg ttgccaaagc
     1861 cgtcagcagg ctgacgatga ccagtgtcag cagcgcgtgg atgcggaatt tgacgattaa
     1921 aatcagaatg aggatgattg ccgccgccga aatgcccaac agggtttgcg cggacagcgt
     1981 ctgtgtccag ccgtccattt gtaaagcctt tctctgtaat aaaagtacaa aattgtcagg
     2041 ttttttaagt tgtgcgaaaa cgatagcaca aatcgggcgg taagttgttt gtctgaagtt
     2101 atattcctgt ttatttgaac gatttatttc agacggcggg aggaaggcgg atacggtatg
     2161 tagtcggatt gaaatatacg gtatgtgggg cttttgggta caatcgcaac atcagtgttt
     2221 caagtgggga aggcgatgga tggacggcgt tgggcggtat ggggtgcttt tgccctgctg
     2281 ccttcggctt ttttggcggt aatggtcgtt gcgcctttgt gggcggtggc ggcgtatgac
     2341 ggtttggcgt ggcgcgcggt gctgtcggat gcctatatgc tcaaacgttt ggcgtggacg
     2401 gtatttcagg cagcggcaac ctgtgtgctg gtgctgcctt tgggcgtgcc tgtcgcgtgg
     2461 gtgctggcgc ggctggcgtt tccggggcgg gctttggtgc tgcgcctgct gatgctgcct
     2521 tttgtgatgc ccacgttggt ggcgggcgtg ggcgtgctgg ctctgtttgg ggcggacggc
     2581 ctgttgtggc gcggacggca ggatacgccg tatctgttgt tgtacggcaa tgtgtttttc
     2641 aaccttcctg tgttggtcag ggcggcgtat caggggtttg tgcaagtgcc tgcggcacgg
     2701 cttcagacgg cacggacgtt gggcgcgggg gcgtggcggc ggttttggga cattgaaatg
     2761 cccgttttgc gcccgtggct tgccggcggc gtgtgccttg tcttcctgta ttgtttttcg
     2821 gggttcgggc tggcattgct gctgggcggc agccgttatg ccacggtcga agtggaaatt
     2881 taccagttgg tcatgtttga actcgatatg gcggttgctt cggtgctggt gtggctggtg
     2941 ttgggggtaa cggcggcggc agggttgctg tatgcgtggt tcggcaggtg cgcggtttcg
     3001 gataaggcgg tttcccctgt gatgccgtcg ccgccgcagt cggtcgggga atatgtgctg
     3061 ctggcgtttg cggcggcggt gttgtctgtg tgctgcctgt ttcctttgtt ggcaattgtt
     3121 gtgaaagcgt ggtcggccgg cgaatcgtgg cgtgtgttaa tggaaagtga aacgtggcag
     3181 gcggtgtgga atactttgcg cttctcggcg gcggcggtgt atgcggcggc ggttttgggt
     3241 gtggtgtatg cggcggcggc gcggcggtcg gcgtggatgc gcgggctgat gtttttgccg
     3301 tttatggtgt cgccggtttg tgtttcggcg ggcgtgctgc tgctttatcc gcagtggacg
     3361 gcttcgttgc cgctgctgct ggcgatgtat gcgctgctgg cgtatccgtt tgtggcaaaa
     3421 gatgttttat cagcctggga tgcactgccg ccggattacg gcagggcggc ggcgggtttg
     3481 ggtgcaaacg gctttcagac ggcatgccgc atcacgttcc ccctcttgaa accggcgttg
     3541 cggcgcggtc tgactttggc ggcggcgacg tgtgtgggcg aatttgcggc gacattgttt
     3601 ctgtcgcgtc cggaatggca gacgctgacg actttgattt atgcctattt gggacgcgcg
     3661 ggtgaggata attacgcgcg ggcgatggtg ctgacattgc tgttggcggc gttcgcgctg
     3721 ggtattttcc tgctgttgga cggcggcgaa ggcggaaaac ggacggaaac gttataatgc
     3781 gaaccctttt ttagaggacg gcaaaatgag cgagttggat aacatccttg cccataaccg
     3841 gcagtttgtc gagtcgggcg aatatgaaaa atactttacc gacaaatacc ccgaacgcgg
     3901 gctggcagtt ttgtcctgta tggatgcgcg gattatcggg ctgctgcccg acgcgttggg
     3961 tttgaaaaac ggcgatgcca agctgattaa aaatgccggc gcgctggtta cgcacccgtg
     4021 gggttcggtg atgcggagcc ttttggttgc cgtgtttgaa ctgaaggtca gagagattat
     4081 ggtcatcgcc catcacgatt gcggtatgca ggggctgaat gccgaagaat tcctcgggcg
     4141 cgtccgggaa agccggattc ccgaagaccg tatcgaaacc ctgcgttatg ccggtatcga
     4201 cctcgacggc tggctgaccg gtttcgacaa cgtcgaagac agcgtgcgcc acacggtgga
     4261 ccttatccgt aaccatccgc tgatgccgcg ccatatcgcc gttcacggac tggtcatcca
     4321 tcccgttacc ggcaaactga cgctggttgt ggacggcagt gtttcagacg gcatggactt
     4381 atcggaagga atggaaacat catgaagaaa atcggactgt tcggcggcac tttcgacccg
     4441 atacacaacg ggcatctcca catcgcccgc gcctttgccg acgaaatcgg cttggacgcg
     4501 gttgttttcc tgccgacagg cggcccgtat cacaaagacg cagcctccgc ttccgccgcc
     4561 gaccgccttg ccatggtcga actggcgacg gcagaagacg cgcgttttgc cgtcagcgat
     4621 tgcgacatcg tccgagaagg tgcaacctat acttttgata ccgtccaaat cttccgccag
     4681 cagttcccat ccgcgcaact ctggtggctg atgggcagcg acagcctgat gaagctgcac
     4741 acatggaaaa aatggcagat gctcgtgcgc gaaaccaata tcgccgtcgc catgcggcag
     4801 ggcgacagcc tgcaccaaac cccgcgcgaa ctgcacgcgt ggctgggcaa gtcccttcag
     4861 gacggcagcg tccgcatctt gtccgccccg atgcataatg tgtcgtcaac ggaaatccgc
     4921 cgcaaccttg ccagccaagg cgtttcagac ggcatcccgc ctgccgccgc acgctacatc
     4981 cgcgaacacg gtttgtatga aaaataaagt caaatcagta agaaacggct ataatgccgt
     5041 ctgaaaacat cccgttagga aaataatgaa cgaacaagaa ctgcaagacc tgcaaaaaat
     5101 ggtcggggtc gccgtcaacg ccctcgaaga catcaaagcc aaagacattt ccgttctcga
     5161 aacgcaagac aaaacttcgc tgtttgccag aatgattatc gccagcggcg acagtacgcg
     5221 ccaagtcaaa gcactggcca acaacgttgc cgtcgatttg aaagaagccg gttttgaaat
     5281 cctcagcacc gaaggcgaca gcggcgaatg gacgttggtt gatgcaggag acctcgtcgt
     5341 ccacgttatg ctccccgccg tgcgcgactt ctatgatatc gacacccttt ggggcggcga
     5401 gaaaccgagt ttccacgccg gaatgcagaa gccgtggcac gcggcagact gattcccgat
     5461 gccgtctgaa tgttcagacg gcattttcct gtaagggaga aagcattgaa catcaccgtt
     5521 ttggcagtcg gcaccaaaat gccgcgctgg gttgatgagg ccgtcgccga atacgccaaa
     5581 cgcttcggac gcgacgtcgc ctacgcactc aaagaaatca aacccgaaaa acgcggcgcg
     5641 ggcgtgaatg ccgcccaagg tatggcggcg gaagaaaaac gcatccttga agccgttccg
     5701 caaggcgcgt tccttgtcgt tcttgacgaa cgcggcaaag caccgacctc cgtcgagctg
     5761 gcggaacacc tcaaaagctg gcagcaaaac ggcgaacacg tctgcttcgt catcggcggt
     5821 gcggacggca tgaccgaccg cctcaaacaa caagcccgca tgatgatgcg cctgtccagc
     5881 ctcaccctgc cgcacggcat ggtgcgcgtc cttctgaccg aacaactcta ccgcgccgcc
     5941 tccatcctgc acaaccaccc ctaccatcgg gaataagaag gcttttgtct gtacccctaa
     6001 tcggttaaaa tcaccccgtt atttctgaac acacagcaaa ggaatccgct atggcacgca
     6061 tggtattctg cgttaagctc aacaaagaag ccgaaggcat gaaatttccg ccgcttccca
     6121 acgaattggg caagcgcatt tttgaaaacg tatcgcaaga agcatggatg gcgtggacgc
     6181 gccaccaaac gatgctgatt aacgaaaacc gcttaagcct tgccgatccg cgcgcgcgcg
     6241 aatatctggc gcagcagatg gagcagtatt tcttcggcga cggcgcggat gccgttcagg
     6301 gatatgttcc gcaataacgg ttttccgttt tgaacacagg ctgtccgaac ctgcttcaga
     6361 cggcctttaa aatacgccgg acaactttat cttcacgcaa ccggacaaat tttgacattg
     6421 ggcaatgtta aaatcccccg ttatttccaa ccgcgctttc aggagcagat gatatgcaac
     6481 acgacgttta cgactacacc gcgcatacgg tttctaaaaa caccgtcctg cagaaaacct
     6541 accgcctgct cggattttca ttcattccgg cagccgcagg cgcggcactt gccgccaatg
     6601 ccggcttcaa tttttacgca gccttcggtt cgcgctggat agggtttgcc gtcgtgctgg
     6661 cgtttttcta cggcatgatc cacttcatcg agaaaaaccg ttacagcaat accggcgtta
     6721 ccctgctgat gatattcaca ttcggtatgg gcgtattgat cggccccgtg ctgcaatacg
     6781 cactccatat tgccgacggt acgaaaatcg tcggcattgc cgccgcgatg accgccgccg
     6841 tctttttaac gatgtccgca ttggcacgcc gaacccggct cgatatgaac gcgctcggac
     6901 gcttcctgac cgtaggtgcg gtcattctga tggtcgccgt ggttgccaat ctgtttttgg
     6961 gtattcccgc acttgccttg accatttccg ccggttttgt cttgttcagt tccttaatga
     7021 ttatgtggca ggtacgcacc gtcatcgacg gcggcgaaga cagccacatc agcgcggcac
     7081 tgacactgtt tatctcgctt tacaacatct tcagcagcct gctcaacatc ctgttgtctt
     7141 taaacggcga ggattgatgt cggcaaaacg ccgtccgact atgccgtctg aaaatgcttc
     7201 agacggcatt ttactttggg catacaatac ggatgcacac gcaccaacac acagccgact
     7261 atgaagccgg gaggaatcaa atgttaccga atacaccgcg ccgcgccgtt tatgccggca
     7321 gtttcgatcc gcccacattg gggcatctgt ggatgatacg gcaggcgcaa tctatgtttg
     7381 acgaactcat cgtcgccatc ggcattaacc ccgacaaacg cagcacctat accgtcgctg
     7441 aaaggcagga tatgttgtgc gatattacta aaatgtttcc caacgtcaga accgatgtat
     7501 ttgaaaaccg atttctggtg cattacgccc gtgaggtaga tgcaggattc atcgtgcgcg
     7561 gcatccgttc tgcttcggat tacgaatacg aacgttccat gcgccatatc aacagcgacc
     7621 tcgcccccga aatatccacc gtattcctca tgccgccgcg cgaaatcgcc gaagtgtcgt
     7681 ccactatggt caaaggactg gtcgggccgg aaggctggat ggaaacgatc caccgctacg
     7741 tgccgcaagc tgtgtacgaa aaaatccttg ccgaacatca acacgaaaat tgaacccttc
     7801 tatttttgca aggcatatat ggatatgttg gaaattttgt tccgccatca agacttcgtc
     7861 gccatcaaca aaccgggcgg catatccgtc caccaagaca gcggcgagac cggactggcc
     7921 cgaacactcg ccatacagtt gggcgtagag cgtgtgtggc tgctgcaccg attggacaaa
     7981 cagaccagcg gcattttact gtttgccctc aacaaagaaa gcgcgtccgc ccttagcgga
     8041 cagtttgccg gcaaaagcat caaaaaaacc tatttggcac tatccgaccg caaaccgtcc
     8101 aaaaagcagg gttggattaa aggcggaatg gaaaaatcca gacgcggaat gtggaagttg
     8161 acgcgcaata cagaaaatat cgccgttacc cgattccaca gcatcagtat cgccgaaaaa
     8221 ctgcggctgt tcatcctgga accgcatacg ggcaaaacgc accaattgag agtggcgatg
     8281 aaaagtttgg gcagtccgat tctgggcgac agcttatacg gcggaacaga atccgaaacc
     8341 atgtttctgt atgcatggaa aatacaattt gcctaccaaa accgacaaat cgaaattgtt
     8401 gcacccttaa aaaacgaatg gcagactgaa aatatctctc atgcgctgga agaattttat
     8461 atggcaaaag caggttaata aatataaatt aatttataaa caatatgata aatattttta
     8521 taaaaatttt tcaagaaaac ggttgactgt aaatctcttc a
//
LOCUS       DAXHRI010000080         8495 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXHRI010000080 DAXHRI010000000
VERSION     DAXHRI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.080"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            78..1262
                     /gene="coaBC"
                     /locus_tag="ACFM75_001327"
     CDS             78..1262
                     /gene="coaBC"
                     /locus_tag="ACFM75_001327"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC6255865.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDEELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1329..3485)
                     /locus_tag="ACFM75_001328"
     CDS             complement(1329..3485)
                     /locus_tag="ACFM75_001328"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6255866.1"
                     /translation="MPVPQPTAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLAVWHMDVQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKMVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3578..3784)
                     /gene="rpoZ"
                     /locus_tag="ACFM75_001329"
     CDS             complement(3578..3784)
                     /gene="rpoZ"
                     /locus_tag="ACFM75_001329"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC6255867.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3843..4460)
                     /gene="gmk"
                     /locus_tag="ACFM75_001330"
     CDS             complement(3843..4460)
                     /gene="gmk"
                     /locus_tag="ACFM75_001330"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC6255868.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSQEVIQRRLAKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4588..5187
                     /locus_tag="ACFM75_001331"
     CDS             4588..5187
                     /locus_tag="ACFM75_001331"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC6255869.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5233..6021)
                     /locus_tag="ACFM75_001332"
     CDS             complement(5233..6021)
                     /locus_tag="ACFM75_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC6255870.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAVKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6346..7701
                     /gene="yegQ"
                     /locus_tag="ACFM75_001333"
     CDS             6346..7701
                     /gene="yegQ"
                     /locus_tag="ACFM75_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC6255871.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDILEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7835..8311
                     /locus_tag="ACFM75_001334"
     CDS             7835..8311
                     /locus_tag="ACFM75_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC6255872.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPIANGRNLVTRQAANSVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT         2156 a         2322 c         2015 g         2002 t
ORIGIN      
        1 tccaaaacgg taactaatgt atccgtactt tgttatagaa tggctgctgt ttttcttcgt
       61 aattagaaat tgtcaaaatg ggcaaacata ttcttttagg tgtaacgggc agtattgcgg
      121 cgtataagtc ttgcgagttg gtgcgactgc tgaaaaaaca ggggcattcg gttacggtgg
      181 ttatgagccg ctcggcaact gaatttgttt ctccgctgac ttttcaggct ttaagcggca
      241 atcctgtcct gaccgacacg cacggcggca acggttcaaa cggtatggaa catatcaacc
      301 tgacccggaa tgcggatgtt tttctgattg cgccggcaag tatgaatacc gtggcaaaaa
      361 tctgtaacgg cgtggcagat aacctactga ccagtctggc agccgcacgg aaatgtccgc
      421 ttgccatcgc tcccgcgatg aatgtggaaa tgtggctcaa ccctgccaac caacggaata
      481 tcgcacaact ggtttcagac ggcattactg tctatatgcc gggcttgggc gaacaggctt
      541 gcggagaaaa tggcatggga aggatgccgg aacctgccga attgctggat ttgcttccgg
      601 acttgtggac accgaaaatt ttaaggggca agaaaatctt gattaccgca ggtgcgacat
      661 ttgaagccat tgaccctgtc aggggcatca caaatacctc cagcggaaaa atgggggtag
      721 ctttggcgcg ggcgtgccgt gccgccggtg cggaagtcag cctgattcac ggacagcttc
      781 aaaccgcgct gcctttcggc atatccgata cggttcaagc cgtcagcgct gaagatatgc
      841 atcgcgcagt gcatcgttta atcgacaaac aagatgcttt tatttctgtt gccgccgtct
      901 cagactataa ggttaaaaac aggagtacgc aaaaattcaa aaaagataaa aatgccaaac
      961 cattatccat cgaattggat gagaaccccg atattttggc ttctattgcc tcattgccga
     1021 acccgccgtt ctgcatcggt tttgccgctg aaacggagaa tgtaatggca tatgcgcgag
     1081 aaaaacgtat taagaaaaaa ataccagtga tcgttgccaa tgatgtttca atcgcgatgg
     1141 gcaaaccgac caaccggatt accattatcg gggacgacga ggaactgtct tttcccgaaa
     1201 caagtaaaga tgaagcggca atgcagattg ttgaaaggct tgccgtatat ttgaacaaat
     1261 aagcaattga acggataaac cataaagcgg gttgcctgtt aatcaaaagg caacccgttt
     1321 tacctgcttc aacttctgat gactttgcgg atatatggaa tactatgcag attttgaata
     1381 atctgattca attgattcag attcttgact ttcaataaga atttgaattc gacaaaacct
     1441 tccgttcccg actgggattt agacggtgtt tcgaccgact caatgtccgc accggaatcg
     1501 gaaatcgctt gcgccattaa tgccagcaag ccgtggctgt cttccgattg gacttgaagc
     1561 ccgacacggt agttctgccc gttcatattt tcccagtctg catccagctg ctgttcggga
     1621 tcggacttta acaacgtcgg gcaggtatcc ctatggataa tcatgccttt tcccttaacc
     1681 aacagcaaac ggatggaatc gccgggaacc ggatggcagc actctgcaaa atgaatatgc
     1741 ccgctttcct gaccatcaac tttaatggaa ctgagcctga cctcgctgcc gaaatgctcc
     1801 cctgccaact cggcaatgtg catggcgaca taaacaggca gggtatgccc cataccgacg
     1861 ttgtacagca cttcctcaaa tgaagtctgt ttgtcgttga gatcggcaag gtatttttct
     1921 ttaatgccgt ctgaaagcag gacatctttg ggcagcaaac tggacagggc tttttgtaag
     1981 aggctctctc ccaaaacgac cgcatcgtgc cggttaaggt ttttaatata ttggcgtatg
     2041 gcactgcgcg ccctgcctga cacggcgaaa ttcaaccatg caggattggg tttggcatgt
     2101 tcggatgtaa taatttcgac ggaatcgccg gttttgagct tcgtacgcaa cggcatcatg
     2161 atattgttga tacgtgcggc aacggttttg tgcccgatat cggtatgcac cgcataagca
     2221 aaatcgacag gcgttgcccc tttgggcaaa gttaggattt ttccttttgg cgtaaggatg
     2281 tagatttcgt tcggaaacaa atcgactttg acgtgttcga gaaactcaat ggcattggca
     2341 ctgcttgcct gcaaatctaa gatatttttc agccaccggt ttgtgtgaag caccgcctga
     2401 tcgaccatct tagaatatga tttatagctc caatgtccgg cgattccacc ttcggcaaca
     2461 gcatccattt ccttggtacg tatctgaact tcaatcggca agccgtaagg gccgaccaaa
     2521 gtcgtatgca gactttgata cccgttgctt ttcggaatgg cgatatagtc tttgaaccgc
     2581 ccgggcttgg gctgatagag ggtgtgcaat gcgccgagtg cggcataaca ggctggaatg
     2641 ctgttgacaa tgacgcggaa accgtaaata tccataacct cggcaaagcg cagctttttc
     2701 gccatcattt tctgatggat gccgtacagg tttttttccc tgcctttgat tttggcctct
     2761 atattcgcgc ctaccagccg ctggccgaat gcgcgcaaga ctttgccgac aacgtcctgc
     2821 cggttcttcc ggctcttgtc catcgctttt tttaaagtct cgtagcggtt gggatgcagg
     2881 ttttggaacg ataaatcctg aagctcttga tatgcgttat tcaaacctat acggttggca
     2941 atctgtgcat agatttcaag ggtttccctt gcaatccggc ggcgtttgtc cgggcgcatc
     3001 gaaccgagcg tccgcatatt gtgcaggcgg tcggcaagtt tgacgacgat gacgcgcaca
     3061 tctttggtca tcgccaaaat cagtttgcgg aaactctccg cctgatgttc cgcatgatct
     3121 tcaaatttga gtttttcgag tttggacagc ccgtccacca tctcggcaat cgtattgccg
     3181 aacaccgccg ccatttcccc ttttgtcacg cccgtatctt ccaatacgtc gtgcattaca
     3241 cctgcacaaa gaccctgtac gtccatatgc caaaccgcaa gctgtgtcgc aacggcaatc
     3301 ggatgcgtaa tgtatggttc gccacttttg cgggtttgcc cgtcgtgggc gcgaaacgca
     3361 taggcgacgg ctttttcaag ctccgcctgt tcctcgggct tgaggtagga ggctgtatgg
     3421 aaaagcaggg tacgcgcttc ggcggtcaga gggtcgtaag gtgcggtggg ttgagggaca
     3481 ggcatttcag acggctttcg gaacatattt tatttttcgt ttcaaaccgt cggactgcac
     3541 ggcggcagaa tgtgccgacg tgcgtttccg gcagaattta tttattgcgc gtcaacagtt
     3601 ccgtaccgat atgcccggcg gcgatttccc ttaaggcggt aacggtcggt ttgttattgc
     3661 ggacatcgtc cacaagcggc gtattgccat tttcaagctg gcgggcccga cgggccgcta
     3721 ccaatgtcag gtcaaaatgg ttggaaattt ttccggtaca gtcttcggtg gtaatacgtg
     3781 ccatattatt tgctttcttt caaaaatatt taaaatagga aactgggtat tttcgcggtt
     3841 ttttaggaat tttccaacaa atctgcaata aatcccagtt gccgcgacct tttcagacgg
     3901 caggcattca caatatggcg caaatcctcc tccgcccgcg ccaagtcgtc attgaccacg
     3961 acaaagtcga acaatacgga ctgttcgatt tcatgccttg ccttcgcaag cctcctttgg
     4021 ataacttcct gactgtccgt cccgcgtccg ttgagacgcg cggcaagcac atcgaaagac
     4081 ggcggcagga taaagatgcc gacagcttca ggcagcgcat ctcgaacctg cgccgcgccc
     4141 tgaacgtcga tttccaaaat cacgtcataa cctgccgccg ccaacgcatt cacgccctcc
     4201 gcgcttgtgc cgtaatagtt gccgaatacg tctgcgtatt ccaaaaaagc ctcctgtgcg
     4261 ataagcgact caaactcttc tttggaaaca aagtgataat gtacgccgtt tgcttcgcct
     4321 tcacgcggcg ggcgcgtcgt gtgcgacacg gaaacgcgca aaccgttatg gtttgccaac
     4381 agccgcgaca ccagcgtggt tttgcccgtg ccggaagcgg ccgaaatgat aaagatgttg
     4441 ccttttcgat aagcggacat attttttacc tgtatatttt ccagccgatt gtatcacaat
     4501 ggacacccag tttcctattt gccgatgccc atattttgcc gctattgttt tgattgattt
     4561 ggcaagcggc aggctgacgg ctacaatatg gcgttaaaaa catcaaactt ggaacacgca
     4621 atgctggttc atcccgaagc tatgagtgtc ggcgcgcttg ccgacaaaat ccgcaaaatc
     4681 gaaaactggc cgcaaaaagg catcttattc cacgacatca cgcccgtcct gcaaagtgcg
     4741 gaatacttcc gccttttagt tgatttattg gtttaccgct atatggatca gaaaatcgac
     4801 atcgttgccg gtttggacgc gcgcggcttc attatcggcg cggcactcgc ctaccatctc
     4861 aacgtcggtt ttgtccccat ccgcaaaaaa ggcaagctgc cttttgaaac cgtatcgcaa
     4921 agctacgcgc tcgaatacgg ggaagctgcg gtggaaatcc acaccgatgc cgtcaaaccc
     4981 ggttcgcgcg tgctgctggt cgatgatttg gttgccacgg gcggcacaat gcttgccggg
     5041 ctggaactga tccgcaaact cggcggagaa attgtcgaag ccgccgccat tttggaattt
     5101 accgaccttc aaggcggcaa gaatatccgt gcaagcggcg cgcccttatt taccctgctt
     5161 caaaacgaag gctgtatgaa gggctgaaaa ccgaccctgc cgtctgaaac cggcagggtt
     5221 gttatgatgc gttcaaatca cgcccaaatc ttgcaagccc ctcaacacgc cgtcttcatc
     5281 aacgccgggg caaacatatt tcgccgcctc tttcactgcc tgttccccgt tacccattgc
     5341 cacaccgaac ccgacttcag acagcatttc cacatcgttc aaaccgtcgc cgaacgccat
     5401 cacgtccgcc atttccaaac ccaatgcttc aaccacgctt ctgatgccgt ctgttttcga
     5461 cattcccgca ggcagcagat cgaccgcttc ctcgtgccag cgcaccgttt tcaagccttc
     5521 ccgttccaca atatccgacc agggcggcat ttcgttttcc tccgcaaaca ccagcatctg
     5581 atacaccggt ttgcttgaaa aataatcctt atcggcaaaa aaatcgctgg cgatatgctg
     5641 caaggcgcgg cacacgcatt ccgacagcgc ggacacggcg atcccctctc cgccgacaaa
     5701 cgcataatcc atgcccaagc catccaaatg cgcgcaaacc ctgcccatca aaccggcatc
     5761 catcggtact tcgcgcacag tttttccgtg cagcagcgca aactgtccgt ttatcgttac
     5821 cacggcatcc atgcccgttt ccgccatcat gtccctgacc ttttcgggaa tcgtcgccaa
     5881 agaccgcccc gttgccaacg ccgtcaatat gcctttgccg cgcaaagccg ccaccgccgt
     5941 tttcacggaa ggacgcaagg tatccgtata tttgcggtac agcgtatcgt caatatcgaa
     6001 aaacacgatt ttaggattca tcacattctc tctcgcattc aaactgccgc attgtatccc
     6061 aatcaggcaa atacttgata aatccttata aatttcccgg caaaattgac cgcgaacacg
     6121 aaaaggcgga taatccgccc atcctcaaac ccttttcaga cggcatttgc agcaatgccg
     6181 tctgaaacat ttttacaaag cacacaaatc atgtttcaac acacaggacg acacataaag
     6241 cgtcgcccta tatgttgccc tgattcgaaa ggggttacac ccctcccaaa taaagtctga
     6301 tcctgccgcc ctaaagggcg gggtttcaac cgaaaaggaa acacgatgaa agcacccgaa
     6361 ctcttattgc ccgccggcgg attggaaaga atgcgcgccg cctacgacta cggcgcagat
     6421 gccgtttatg ccggcagccc gcgttactcc ctgcgcgccc gcaacaacga atttgccaaa
     6481 ctcgacatcc tcgagcaagg cattaaagaa gcgcacgaac gcaacaaaaa attcttcctt
     6541 accgtcaata cgctgccgca caattccaaa ctcaaaacct ttgtttccga tatggagccc
     6601 ttgattgcga tgaagcccga cgcgctgatt atggcggatc cgggtttgat tatgaccgtg
     6661 cgcgaaaaat ggccggaaat gccgatccac ttgtccgtac aggcaaacac caccaactac
     6721 tggggcgtaa aattctggca aaacatcggt gtcgaacgca ttattctgtc acgcgaattg
     6781 agcatggaag aaattgccga aatccgccaa gaatgccccg acatcgaact cgaagtcttc
     6841 atccacggcg cattgtgcat cgcctactcc ggccgctgcc tgttgtcggg ctatttcaac
     6901 caccgcgatc ccaaccaagg cacctgcacc aactcctgcc gttgggacta caaagtccac
     6961 aacgctacgg aaagcgatgc gggcgatgcc cagcttctgc aaggtttcaa ctttgaaaaa
     7021 gcccaagaag aagccaacca aaactttgaa ggcatcaacg gtcaaaaacg ccatccctac
     7081 gccgacaagg ttttcctgat tgaagaatcc aaccgtccgg gcgagatgat gccgattatg
     7141 gaagacgaac acggcaccta catcatgaac tccaaagacc ttcgtggcat tgaagtggtt
     7201 gagaaactcg ccaaaatcgg cgtggacagc ctcaaagtcg aaggccgcac caagtcgctc
     7261 tattatgtcg cacgcgtcgc ccaatcctac cgcaaagcga ttgacgatgc cgtcgcaggc
     7321 cgtccgtttg attacagcct gttgagcgaa ctcgaaggcc ttgccaaccg cggctacacc
     7381 agcggcttcc tcgaacgcca ccacactcag gattatcaaa actacctgac cggccattcc
     7441 accgccaaac aaagccaata cgtcggacac gttaccgaaa tcgatgaaaa cggctgggca
     7501 acagtggaag tcaaaaaccg ctttgccgtc ggcgattcgc tcgaaatcat ccacccgaac
     7561 ggcaaccaaa ccatcaaatt ggaacaaatg acccgcaaag gccaacctgt cgatgtcgca
     7621 cccggcaacg gcattcaggt caaaatcccc gatatgcagg gcaaagaaaa agccctcatc
     7681 gcacgcgtat tgaacccctg agccacaatg ccgtccgaac cccgttttca gacggcattt
     7741 tgttgaatct tacggtacaa tggcaatgat ttcccacagc aaacaaggac atccgacatg
     7801 aaaaaaaatc ctcatactct ccgccgccgc cctgatgctg gcagcctgcc aatccggcag
     7861 cgcacctaaa aacgcgcaca cggcatccgc cgacacaccg gccggagaat catgcaaagt
     7921 catccgggac gacaccgcag gaagcggcaa aaacatcatt taccgttgct gcaacggtca
     7981 ggcggcaata aatgcagcca ttgcagacgg cgtattggaa tcagggattc ccgtcagctt
     8041 cggcggcggg ggcgtaccga ttgcaaacgg caggaatctg gttacacggc aagcagcaaa
     8101 cagcgttggc aaatccgact ccgaggcctg cgaacacgcc ctaatcaatg ccgcccgcaa
     8161 attccagcag accgccggca aactcggcgg gcgcagtgtt accggcttcc acagctactt
     8221 cgacaagcaa ccgctgcaag gaggacaata cgactgtcag gcaggctcgt tccacgtccg
     8281 cgtggtcatg cgcgccaatg tcgtccgtta acatatcggc gaccaaaaaa ttaggctgtg
     8341 ttttagtaat ctgttgattt caattatttg caaaggaaaa gacaattatt ttttcggtta
     8401 ggaagagacc tatcctattg aatacattta agttaaatac gtctatcaac actatattat
     8461 agtggattaa caaaaatcag gacaaggcga cgaag
//
LOCUS       DAXHRI010000081         8415 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXHRI010000081 DAXHRI010000000
VERSION     DAXHRI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.081"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            132..341
                     /locus_tag="ACFM75_001335"
     CDS             132..341
                     /locus_tag="ACFM75_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC6255873.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            430..1644
                     /locus_tag="ACFM75_001336"
     CDS             430..1644
                     /locus_tag="ACFM75_001336"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC6255874.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLDDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1731..4310)
                     /gene="clpB"
                     /locus_tag="ACFM75_001337"
     CDS             complement(1731..4310)
                     /gene="clpB"
                     /locus_tag="ACFM75_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC6255875.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4571..5581
                     /gene="trpS"
                     /locus_tag="ACFM75_001338"
     CDS             4571..5581
                     /gene="trpS"
                     /locus_tag="ACFM75_001338"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC6255876.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNLDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLNPEQTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5587..5928
                     /locus_tag="ACFM75_001339"
     CDS             5587..5928
                     /locus_tag="ACFM75_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255877.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6248..6571
                     /locus_tag="ACFM75_001340"
     CDS             6248..6571
                     /locus_tag="ACFM75_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255878.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(6780..8288)
                     /gene="ppx"
                     /locus_tag="ACFM75_001341"
     CDS             complement(6780..8288)
                     /gene="ppx"
                     /locus_tag="ACFM75_001341"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC6255879.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPVGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDSAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
BASE COUNT         1962 a         2153 c         2218 g         2082 t
ORIGIN      
        1 cggcttcgcc gccttgtcct gatttttgtt aatccgctat atcgatttga atttttcaga
       61 aaatgaagcg gacgtttggg cggaggcaac ttgtttgata agatagcaat atttcaaaac
      121 ggagaaagat catgccttat gtcaatatta aagtaaccgg cggcaaggaa gcaccgactg
      181 ccgcgcaaaa agcggaactg atcggcggcg taaccgaatt gctggcacgc gtgctgggca
      241 aaaatcccga aacaacggtt gtcgtgattg atgaagtgga taccgataac tggggaatag
      301 gcggcaaaag cgtcagcgaa cggcgcaaag agggcaggta aaaagcctga aaatctcggt
      361 ttgatgcttt aaattccgcg tgaaaaagag tacattccca cccattgccc aaaatttacg
      421 gaacacatta tggataaatt tcccaagtct gcaaagctcg atcacgtctg ttacgacata
      481 cgcgggccgg ttcacaaaaa agcccttcag ttggaagagg agggcaataa aatccttaaa
      541 ctcaatatcg gcaaccctgc gccgttcggc tttgaagccc ctgatgaaat cttggtcgat
      601 gtcatccgca acctgccgac ttcgcaaggc tattgcgatt ctaaagggct gtattccgcc
      661 cgcaaagcca ttgttcacta ctatcagacc aaaggtttgc gcgatattac ggttgatgat
      721 gtctatatcg gcaacggcgt gtccgagctg attacgatgt ctatgcaggc attgctcgac
      781 gacggcgacg aaatcctgat tcccgcgccc gactaccctt tgtggacggc ggcggcaacg
      841 cttgcgggcg gtacggtacg ccattatctg tgcgacgaag aaaacggctg gttccccaac
      901 cttgccgata tggaagccaa aatcacgccc aaaaccaaag ccatcgtcgt catcaatccc
      961 aataatccga caggcgcggt gtacagcagg gaaatcctgt tggaaatcgc cgaactggcg
     1021 cgcaagcacg gtttgattat tttcgccgac gagatttacg ataaaatcct ttatgacggc
     1081 gcggttcacc accacatcgc cgcgcttgcc cccgaccttt tgacggtaac gttcaacggt
     1141 ttgtccaaat cctaccgcgt cgccggcttc cgccaaggct ggatggtgct caacgggccg
     1201 aaacatcacg caaaaggtta catcgagggt ttggatatgc tctcgtccat gcgcttgtgt
     1261 gccaatacgc cgatgcagca cgcgattcag acggcattgg gcggctatca gagcattaac
     1321 gaatttatcc tgccgggcgg gcggcttttg gaacagcgca acagggcgtg ggaactggtc
     1381 agccaaatcc ccggcgtgtc ctgcgtcaaa ccgatgggcg cgatgtatat gttcccgaaa
     1441 atcgataccg aaatgtaccg catccgcgat gacatgaaat tcgtttacga tttgctggtg
     1501 cgcgaaaaag tcttgctggt acagggaacg gggtttaatt ggattaaacc tgaccatttc
     1561 cgcattgtca cgctgcctta cgtccatcag attgaagagg cgatgggcag gttggcaaga
     1621 ttcctgcaaa cctaccgcca ataaggggac ggtttgtctg ccgaggataa aaaatgccgt
     1681 ctgaaacgga gattcccgtt tcagacggca ttttcaacag caggaacgaa tcaggcaaat
     1741 ttcagtctgt cgccgtcggc ttccaccctg atttcgcttt cgggcgcata gtttccggca
     1801 agcagggctt ttgccagcgg gttttcgatt tccgactgga tggcgcgttt gagcggacgt
     1861 gcgccgtaaa tcgggtcgaa accggctttg gcgatgatgt ccagtgcggc atcggaaaca
     1921 gtcaggcgca ggttttgttt ttccaaacgt ttttccaaac ctttaagctg gattttcgca
     1981 atgctacgga tgtttgcctg atccaaaccg tggaacacga ccacttcgtc aatacggttg
     2041 atcatttcag gacggaaatg ttctttcaca tcctccatca caacttcttt caccgcttcg
     2101 tagtcctgcg tgcccatttg ttggatgtgc tggctgccga tgttggaagt catgacgata
     2161 acagtgtttt tgaagtccac ggtgcgacct tgcccgtcgg tcaaacgacc gtcatccaat
     2221 acttgcagca ggatgttgaa cacgtccgga tgggcttttt ccacttcatc caaaagaatc
     2281 acgctgtatg gtttgcggcg gacttgctca gtcaggtagc cgccttcttc gtagccgaca
     2341 tagcccggag gcgcgccgat gaggcgggca acggcgtgtt tttccatata ttcggacata
     2401 tcgatacgaa tcagatgatc ttcactgtca aacaggaagc ctgccaatgc cttacacaac
     2461 tcggttttac ccacgccggt cgggcctagg aacaggaagc tgccgtaggg tttattcggg
     2521 tcggcaagac cggaacggct gcggcggata gcgtcggaca cggcacgcac ggcttcgtct
     2581 tgaccgacta cgcggcggtg caatacttct tccattttca gcagtttgtc gcgttcgcct
     2641 tccatcattt tggatacggg aatgccggtc atacgggaaa ccacctctgc gatttcctct
     2701 gcgccgacat tattacgcaa gagtttgttt gccggttttg tgctgtccgt atctgcccgt
     2761 tcggcggctg cacgctgttt ttccaaatgc tccaaatctt catacatcaa ttttgaagcc
     2821 agtgccaaat cgccttgccg ttttgcctgt tcgattttaa ttttgacttc gtcaatttgt
     2881 ttcttaatat tagcagcacc gtctgaaatt gctttttcgg ctttccagat ttcgtctaaa
     2941 tcggcgtatt ctttttgcag accgttgatt tcctcgtcta tcagttccaa acgttttttg
     3001 ctggcatcgt ctttttcttt ttcaacgtgc gccttttcca tccgaagctg aattagacgg
     3061 cggtcgattt tgtccattgc ttccggcttg gtttcttttt ccatcttgac acggctggcg
     3121 gcttcgtcaa tcaaatcaat cgctttatca ggcaggaagc ggtcggtaat gtagcggtcg
     3181 ctcaactccg ctgcggcaac gatggcgggg tcggtgatgt cgatgccgtg gtggatttcg
     3241 tagcgttcct gcaaaccgcg caagatggcg atggtgtctt ccacgcttgg ctcgccgacc
     3301 aatacttttt ggaagcggcg ttcgagtgcc gcgtcttttt cgatgtattg acggtattcg
     3361 tccaaggtgg tcgcgccgat acagtgcaat tcgccacgtg ccaaagccgg tttcagcata
     3421 ttgcccgcat ccatcgcgcc gtcggttttg cccgcgccga ccaaagtatg gatttcatca
     3481 atgaaaatca gggtgttgcc gtcgtctttc gccaaatcgt tcaacacgcc tttcaagcgt
     3541 tcttcaaatt cgccacggta tttcgcaccg gcaatcaaag ccgccaagtc cagaaccaaa
     3601 aggcgtttgt tacgcaggga ttcaggtact tcgccattga cgatacgttg cgccaagcct
     3661 tcaacaatag cggttttacc cacacccggc tcaccaatca gtacagggtt gtttttggtt
     3721 cggcgttgca atacctgaat cgcacggcgg atttcgtcgt cacggccgat aacggggtcg
     3781 agcttaccgt ctcgggcgcg ttgggtcaga tcgagcgtat attttttcaa agcatcgcgt
     3841 tggtcttcgg cattggcatc gttcacgttt tgtcctcctc gtaccgcgtc aatcgcggca
     3901 ttgatgtttt gttcggtcgc gccggcttct ttcaaaattt tgccggccgc atcgttctgc
     3961 tgcaccaagg caagcaggaa aagttcactg gcgatatagg catcgccgcg tttggttgcg
     4021 gctttgtcca tcaggttcaa caccgcctgc aattcgcggc tgggcagaat gtcgccgccc
     4081 tggccggaca ctttcggcag gctgttcaaa tgctgctgca aacgctgttt cacctgcggc
     4141 acgttcacgc ccgcatgagc caagagcgcg gcagcaccgc tgttttggtc gtcaagcagg
     4201 gctttcagca caaagcccgc ttccagatag ctgccgtccg cagccaacgc caaactctga
     4261 gcttctgcaa gggcctgttg gaatttggcg gttaatttgt cgtatcgcat ttttgtttcc
     4321 ttttcaaaat gtccgctgtc gaagcctata tgtgcataat tgtggataac tcaagttctg
     4381 ttttctgttt ttctatattt aattcgatat atcattgaat ttaaagtata taaaaatgta
     4441 taataatgtg tataactata tcttcttaat atggaaaagt ctgttgtcgg ctggatgtag
     4501 gtggcaaatc gggtataatc ggcgcatctt tttccctttc agacggcatt gatgccgcaa
     4561 ggacattttt atgagcaaaa aacgagttct gaccggcgta accaccaccg gcatcccgca
     4621 tctgggcaac tacgtcggcg ccatccgccc cgccgtccgc gcggcgcaaa acctcgatac
     4681 cgaatcattc ctctttttgg ccgactatca cggtatcatc aaatgtcatg agccggaaat
     4741 gattcatcaa tctactcaag ctgttgccgc cacatggctc gcctgcggac tcaatccgga
     4801 acagactact ttctatcgtc aaagcgacat tcccgaagtg atggagttga actggattct
     4861 gacctgtatc actgcaaaag gcctgatgaa ccgcgcccac gcctacaaag ccgccgtgca
     4921 ggcaaatgcg gaaaacgggc aggaagaccc cgatttcggc gtggaaatgg gtttgttcag
     4981 ctatccgatt ctgatgaccg ccgacatcct tatgttcaac gccaacgaag tgcccgtcgg
     5041 gcgcgaccaa atccaacacg tcgaaatggc gcgcgacatc gccggccgct tcaatcaccg
     5101 ctttaaagaa atctttaccc tgcccgaagt gaaaatcgac gaaaacgtcg aactcttggt
     5161 cggtttggac ggacgcaaaa tgtccaaatc ctacggcaac accattccgc tttgggaaaa
     5221 cgacaaaaaa acccaaaaat cggtcaacaa aatcatcacc aatatgaaag agccgggcga
     5281 gccgaaacag cccgacgaaa gcccattgtt tgaaatctac aaagccttct ccacgccgtc
     5341 tgaaaccgca gcgttcacgc aaatgcttgc cgaaggcttg gcttggggcg aggcaaaaaa
     5401 acttttggcg gcgaaaatca acgccgaact cgccgaaccg cgcgaacgct acaacgagct
     5461 gaccgccgac ccttcgcaaa tcgaagagat tttgcaggca ggcgcgcaaa aagcgcgtaa
     5521 agaagcacgc gaattgttgg acaaagtacg cgatgcggtc ggcatccgcc cgttgaaata
     5581 aacccgatgc cgtccgaacc tcttaaaggc ttgccggaac aaaacgtcgt ccggctgacc
     5641 ggcaagcatc ccaacgactt ggaagccgtc agcggcaaat gcatggaaac cgacgataag
     5701 ggcggtccgg taggctgggc ggaaaacggc gtgtgccata ccttgtttgc caaactggtg
     5761 ggcaatatcg ccgaagacgg cggcaaactg acggattacc tgatttcgca ttccgccctg
     5821 caaccctatc aggcaggcaa aagcggctat gccgccgtgc agaacggacg ctatgtgctg
     5881 gaaatcgaca gcgagggggc gttttatttc cgccgccgcc attattgagg tattcggaca
     5941 tcccggaata tatttgggtt tttcaaacct tgcgaaaaaa agtccgcacc gcagggaaac
     6001 tcaaaaggag ggggatatgt gttacaattt tccgaactgt tttcataaaa tagttttcgg
     6061 acgtgtttca atatggcatt gatgccgcct tattgttcga gaaaaaaacc tttatattta
     6121 aatataatgg gttttaacta aacgggaaac cgttttctct ccggccgatg ggcaaaatca
     6181 gccgattgat ggaacacggt tcgattttta agcaaaacct taatcaacaa ctcaagaagg
     6241 ataagacatg aaaaaattat tgattgccgc aatgatggcg gctgccttgg cagcttgttc
     6301 gcaagaagcc aaacaggagg ttaaggaagc ggttcaagcc gttgagtccg atgttaaaga
     6361 cactgcggct tctgccgccg agtctgccgc ttctgccgtc gaagaagcga aagaccaagt
     6421 caaagatgct gcggctgatg caaaggcaag tgccgaggaa gctgtaactg aagccaaaga
     6481 agctgtaact gaagcagcta aagatacttt gaacaaagct gccgacgcga ctcaggaagc
     6541 ggcagacaaa atgaaagatg ccgccaaata atttgttgcc ttggcaaaaa tgatgggatg
     6601 ccgcctgccg gcaacccaaa cgaaaccgcc tgaagatttt caggcggttt ttgagttgta
     6661 tgccccttgt tttttacgcc tgcatgaccg ttccgagcag atgtgccgcg catatcggga
     6721 tgttgcggac aggcaaatgc cgtctggaca gggtttggac ggcatttgtt ccgtaaggtt
     6781 cagacgacct cgactttaaa cggcatattg attttttgcc attggacgct ttcatattcc
     6841 aatgcgtcgg caatcagggg atggcgttcc agccattccc tgtcaatacg caggatgaag
     6901 ccgcagcttt ccgtatccgt gcgcaactgc atatttttcg ggaaagacag gtcttggcgc
     6961 gaacggcaga acagtgcggc aaggcgcagg gacaaaacgg cataccacaa catttcgttg
     7021 gtgccgatga tgccgctcat ttttttcata tcgccgcgat gaccgatgac cagttgggca
     7081 agtatggttt gttccttgcg tgaaaagccc ggcatatcgg cgttttcgag gatgtaggcg
     7141 gaatgcttgt gatagccggt gtgggcgatg tccaaaccga tttcgtgcaa cgcggcggcg
     7201 cgtccgagat actgttgcca caaggcaagc tcttgaaccg taacattttt agcgtggcag
     7261 aggctgtcca taaaggtttg cgcggtctcg gcggtgcgtt tcgcctgatt gaggctgacg
     7321 tggtagcggt gttggaactc ggcaaccgtt tgtccgcgca tatcttcgtt taaaccgcgc
     7381 ccgatcaaat cgtaaaacac gccgtcgcgc agggcggctt cggttacggt catcctgtcg
     7441 agtttcattt cctcaaacgc cgccatcatc acggcaagtc cgccggcaaa aacttcgatg
     7501 cgttccggtt tcaggttttc aaatttggct tttttgaccg aaccggcttc gatgatgcgt
     7561 tcggcgaggg cgcgcatgcc tttgtaggta atgtccgcct cttggggcat ttcggcggca
     7621 agcacgtcgc ggatggattt tgccgaaccc gatgtgccga cggcggaatc ccaaccttcg
     7681 cgcctcatat ttttgctgat gcgctggatt tcgttgcggg cggcggaaat ggcagattgg
     7741 aagtctttgg cggtgatttt gttttggaag aagcgcaggc tgtaggttac gcagcccaag
     7801 ggcaggcttt cggtaatgtc gggattcagc gtcgaaccga tgacaaattc tgtcgaaccg
     7861 ccgccgatgt cgataaccag cattttgccg ccgaccgggg ggagggtgtg gatcacgccg
     7921 gtataaatca gccgcgcctc ttcgcgcccg gcgatgattt cgatggggaa acccagtgcc
     7981 gcttcggctt tgggaaggaa atctgcgatg tttttggcga cgcggaatgt attggttgcc
     8041 acggcgcgta cctgttcagg gcggaagccg cgcaggcgtt cgccgaattt tgccagacag
     8101 tccaaagcct gttcttggga ggcggcactc aggtttttct gttcgtccag tccggcggcg
     8161 aagcgcacca tctgtttgaa cgaatcgatg acttttaatt gtccgttgtt gttttcgcaa
     8221 atctggaggc ggaaactgtt ggaacccaaa tcgacggagg caaggatgtt tgcgggggtg
     8281 gtggtcatgg cggataccgg tgggggaaaa acgcaatgtt actctgacgg cgcaggcgtt
     8341 gacaataaat gatgcggcgg tttttgattc tgcccacgga tgttgccgac ggcatttttt
     8401 gcgcttattt gaaat
//
LOCUS       DAXHRI010000082         8367 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXHRI010000082 DAXHRI010000000
VERSION     DAXHRI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.082"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(269..1201)
                     /gene="cysK"
                     /locus_tag="ACFM75_001342"
     CDS             complement(269..1201)
                     /gene="cysK"
                     /locus_tag="ACFM75_001342"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC6255880.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVPNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1395..2000)
                     /locus_tag="ACFM75_001343"
     CDS             complement(1395..2000)
                     /locus_tag="ACFM75_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255881.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTFAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1997..2848)
                     /gene="dapF"
                     /locus_tag="ACFM75_001344"
     CDS             complement(1997..2848)
                     /gene="dapF"
                     /locus_tag="ACFM75_001344"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC6255882.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2985..3281
                     /locus_tag="ACFM75_001345"
     CDS             2985..3281
                     /locus_tag="ACFM75_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255883.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3295..3963
                     /locus_tag="ACFM75_001346"
     CDS             3295..3963
                     /locus_tag="ACFM75_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6255884.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLMLGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(4035..6155)
                     /gene="pnp"
                     /locus_tag="ACFM75_001347"
     CDS             complement(4035..6155)
                     /gene="pnp"
                     /locus_tag="ACFM75_001347"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC6255885.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTSV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLKVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6392..7255)
                     /locus_tag="ACFM75_001348"
     CDS             complement(6392..7255)
                     /locus_tag="ACFM75_001348"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC6255886.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERSIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDEEGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7455..8318
                     /gene="rfbD"
                     /locus_tag="ACFM75_001349"
     CDS             7455..8318
                     /gene="rfbD"
                     /locus_tag="ACFM75_001349"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC6255887.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGNNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FVQHIFQAASQQQTSFPIPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1992 a         2416 c         1933 g         2026 t
ORIGIN      
        1 ccgtactatc tgtactgtct gcggcttcat tgccttgtcc tgatttttgt taatccacta
       61 tatctgtact gtctgcggct tcgttgcctt gtcctgattt ttgttaatcc actataaaaa
      121 atatttccct caagcgatgc ggcgacacca acacctgatt aaaacacaac cggcgttttc
      181 ggcatatatg caaaaacgca tttcccagca cggcgttaca ccgataccga aaaatgcgtc
      241 tgaatgtttc gacaatccga ttaaagcatt atgccaaatc tgcaaaaagt ggcgtagaga
      301 gatagcgttc gccataagaa ggcagcagca cgactatcag cttgccttcg ttttcaggct
      361 gtttggcaag ctgcaacgca ctccaaaccg ccgcaccgga agaaataccc accaaaatgc
      421 cttctttttc cgctattgcg cgggcggttt caaaagccgc ttcgttcggc actttggtaa
      481 tgctgtcgta aattttggta ttcaaaacgg tcggaataaa gcctgcgccg atgccttgaa
      541 tcgggtgcgg gcctttttcg ccgccgctca atacggggga agcttcaggc tcgacggcaa
      601 ccactttaac ttcgggtttg tattttttca acacttcgcc cacgccggta atcgtaccgc
      661 ccgtgccgac gccggcaacg aagacatcga ctttgccgtc cgtatcccgc caaatttcct
      721 cggcggttgt tttgcggtgg acttcggggt ttgcctcatt gtcgaactgg cgcggcataa
      781 aataagtgtc gggatgcgcg tccaccaagg atttcgcttt ggcaatcgcg cccgccatac
      841 cttcggcggc aggggtcaga atcagctccg caccaaacgc gcgcaacagc attttgcgct
      901 ccttactcat gctttccggc atggtaatcg ccagcttgta gccgcgtgcg gcacatacca
      961 ttgccaaacc gacacccgta ttgccgctgg ttgcttcgac aatgacggtg tttttgttga
     1021 ttttgcccgc tttttcggca ccctcgatca ttgcttcggc aatgcggtct ttgacgctgc
     1081 tgcctggatt gaaaaattcc agtttcacgg caacctccgc cttcaaacct tcggtcagac
     1141 ggttcagttt gaccaaaggc gtgttgccga ttagttcggt gatgctgttt gcaattttca
     1201 tcttctgctc ctgaataata aggtttcgac agtaaggcgc aatccccata ggcatcctgt
     1261 ccgattgcag gggatttgcg ccgaaacata aacaaatttc ggatatggct ttatcctaaa
     1321 cctatgccgc ctaaaaaacc aatatccaaa atttctttct ttataacttt ttgttccgat
     1381 acggccgcac cgtattatct tccaatttct gaaagcagca gcaccagtac cgagacctgc
     1441 atcaacgctg ccgtcaaccc gccgagtcgc agctgcgagc cgccgaacgt tgacggcggc
     1501 tgccggtcta taccgcagcg gtggacatag gcaaccgcca tcagcacaaa ctgcaacacg
     1561 aaccaataaa ccggcttcag cgcataaagt ttgagcagtg ccgcaaacac aaacacttga
     1621 accgttcccg aaaagcaata atgcaccaac gcacatattc cgacaaaagc aaacgtcatc
     1681 gatacattac tgacggcaaa aagcccgagc agcggatgtt cggggtctgc ctgccatccg
     1741 tttccatctg ttttccgatt ccaatgcccg atgaaaaaaa atatgctgcc caaagtcagg
     1801 taaaaatggg ggatgaacaa gggcgcggcg cgggtcattc cccatatgcc gggcatcagc
     1861 tttgccccca aaaccgatat gcccagccac agcataatac tcgcccaaaa ccattgcaac
     1921 attcctgcgc ggaatatcag aaacagaaaa accgccgcat acagtaaggc agacaaaaaa
     1981 tccaaatgca gcaaaatcat gaatacgcca actcgccttc aaacaccgtt tccgcagggc
     2041 cggtcatcat cacatcgccg ccgcaggccc attcgatata taaagttccg cccggcaaaa
     2101 tcacctctac cgttttccct tcatccaaca accccagacg gatacccgcc accacagccg
     2161 cacacgcgcc cgtaccgcac gcttgggttt cgcccacgcc gcgctcgaac acgcgcaaac
     2221 gaatcgcggt tcggctgaca atctgcataa agccgacgtt gacgcgttcg ggaaactgcc
     2281 tgtgcggttc gataagcgaa ccggtttcgc gcaccggcgc gcattccacg tcatcgacca
     2341 caatcaccgc atgggggttg cccatattga cgcagctgac aggctgaatg ccggattcga
     2401 gatgcacccc gtaaatacag gcatcatccc cctcgcccga ttcggggaca aacggtattt
     2461 cagacggcat aaacttcggt ttgcccatat tgaccgtaac cataccgtta tcggacaatt
     2521 tcggaaaaat aacgccattt gccgtttcaa cacaaatttc tttcttatcg gtcaaaccct
     2581 tgtctgcaac aaaacgggca aaacaacgcg ctccgttgcc gcattgcccg acctcgctgc
     2641 cgtcggcatt gaaaatacgg taacggaaat ccacgccttc ggtttccgaa cgcccgacca
     2701 ccaaaagctg gtcgaagccc acgccccgga aacggtccgc ccatgccgca atcggcgcgt
     2761 cctcgggggt aaaatcctga ctgaccgcat caatcaccat aaaatcgttg cccaaaccgt
     2821 gcattttggt aaatttcaac gtgttcatat tgttccgaaa tcccgtatcg tctgttgctt
     2881 ccgaatccga aatcccccgc accgaaggga aatgcttcca tctcccgccg cctgaggatt
     2941 aaaatcagcc attatactgt ttgttacccc ttacaagaaa caccatgttc cgcatattac
     3001 tgttgctttt cccttgtgca atccttgccg cctgcacccg aaacataccg gcaaacccgg
     3061 ccgtttcaaa ttgcgcgcat cttcaaaacc gcaccatcct gtattttgac gcacacggca
     3121 aacccgccga cggcgcagta aagggcggat tttacaaaat tatcgaaata cagggtgcaa
     3181 actgcatcct tattcaagac tttaacagca cgggcaatgc cctcggcgga tcctatgtgc
     3241 cggactgccg caaatcatcc cttttcccga aaaatatttg acttcaaact gcctatgaac
     3301 cacgacatca ctttcctcac cctgttccta ctcggtttct tcggcggaac gcactgcatc
     3361 ggtatgtgcg gcggattaag cagcgcgttt gcgctccaac tccccccgca tatcaaccgc
     3421 ttttggctga tcctgctgct taacacagga cgggtaagca gctatacggc aatcggcctg
     3481 atgctcggat taatcggaca ggtcggcgtt tcactcgacc aaacccgcgt cctgcagaat
     3541 attttataca cggccgccaa cctcctgctg ctctttttag gcttatactt gagcggtatt
     3601 tcttccttgg cggcaaaaat cgagaaaatc ggcaaaccga tatggcggaa cctgaacccg
     3661 atactcaacc ggctgttacc cataaaatcc atacccgcct gccttgcggt cggaatatta
     3721 tggggctggc tgccgtgcgg actggtttac agcgcgtcgc tttacgcgct gggaagcggt
     3781 agtgctgcaa cgggcgggtt atatatgctt gcctttgcac tgggtacgct gcccaatctt
     3841 ttggcaatcg gcattttttc cctgcaactg aaaaaaatca tgcaaaaccg atatatccgc
     3901 ctgtgtacgg gattatccgt atcattatgg gcattatgga aacttgccgt cctgtggctg
     3961 taagccccat tcattcccga tacccgtaaa aataccgtct gaaacctgtt cagacggcac
     4021 tttcacccta agcgttactc ggcggcattt tcctcacggg caggcgcgtc cagcagggct
     4081 ttgatggaca gacggacacg gcctctgtcg tccacttcca atgctttcac gttcaccacc
     4141 tgaccgactt tcaggtagtc gccgacattg cgtacgcgct cgtgggcgat ttggctgatg
     4201 tgtaccaaac cgtctttgcc cggcatcacg ctgacaatcg cgccgacgtt gttgtcgagg
     4261 attttcacca cagtgccttc atacactttg cccacttcca cttcggcagt aatctgctcg
     4321 atacgttttt tcgccgcatc gccggcttct tgagtggtcg cggcaatggt aatcgtaccg
     4381 tcttcggcaa tattgatttc cgtaccggtt tcagcggtaa tcgcacggat ggtttcaccg
     4441 cccttaccga taacttcgcg gattttgtct tggttgattt tcatcgtgaa caagcgtggc
     4501 gcgtgtgcgg acagctcttg cgggcccgca acggcggctt tcatctgatc caagatgtgc
     4561 agacgcgctt ctttggcctg tgccaaagcg atttgcataa tttctttggt aatgccttgg
     4621 attttgatgt ccatttgcag cgcggtaacg ccttcggtcg taccggccac tttaaagtcc
     4681 atatcgccca agtggtcttc gtcgcccaaa atgtcggtca ggacggcaaa tttgttgcct
     4741 tccagaatca gacccatcgc gataccggca acgtgtgctt tcaaaggcac gccggcagac
     4801 agcaggctca ggcagccgcc gcagacggaa gccatagagg aagagccgtt ggattcggta
     4861 atttcggaga ccacgcgcat ggtgtagctg aaatcttcag gtttcggcaa tacggccaac
     4921 aatgcacgtt tagccaaacg gccgtgaccg atttcacggc gtttcggtgc gcccatgcgg
     4981 cccacttcgc cggtagagta cggcggaaag ttgtagtgca gcataaagcg gtcggtgtat
     5041 tcgccggaca gcgcgtcgat gatttgctcg tcgcgcgaag tacccaaagt tgcaacggcc
     5101 aaagcttggg tttcgccacg ggtaaacaat gcagaaccgt gcgtgcgcgg caatacgctg
     5161 gtttggatgt tcagcggacg gacggtgcgg gtgtcgcggc cgtcgatgcg cggttggccg
     5221 tccaaaattt ggctgcggac gacatcggct tccaagtgtt tgaaaatgcc tttgatttcg
     5281 ttggctgcca gcgtgtcggt ttcttcggta atcaaggctt cttttaccgc actccaagct
     5341 tcgtccaatt tggcagaacg cgcttgtttt tgacggattt tgaacgcttc tttaatggtt
     5401 tcgccggcaa tcccgcggac tttggcaacc agttcctcat tggtttcagg tgctttccaa
     5461 tcccaaagtt ccggattgac ttcgtcggca aattcattga ttgcattgat ggcaacctgc
     5521 atttgatcgt ggccgtaaac caccgcgccc agcatcacgt cttcgggcag gattttggct
     5581 tcggattcca ccatcaacac ggcttttgaa gtaccggcga ccaccaagtc caattgcgat
     5641 ttcgccaact cggctttagt cgggttcaaa acgtacacgc cgtttacata acccacgcgt
     5701 gctgcgccaa tcggaccggc aaacggtacg ccgctcaaca ccagcgcggc agacgcgccc
     5761 aacattgcag gaatgtcgga atcgatttca ggatcgacgg acacgaccat cgctacgatt
     5821 tggatgtcgt ggtagaaacc ttcggggaac agcggacgaa tcggacggtc aatcagacgg
     5881 ctggtcagga tttctttttc gctttgtttg ccttcgcgtt tgaagaaacc gccggggatt
     5941 ttacctgcgg cgtaagtgcg ttccaaataa tcgacggtca gggggaagaa gtcttggcct
     6001 tctttcactt ctttgttggt ggtaacggca accaaaacaa cggtgtcgcc catagagact
     6061 ttaacggcag cggcggcttg gcgggcaatt tcgccggttt ccaaagtaac ggtgtggttg
     6121 ccgtattgga aagttttaac gtgtttgtcg aacattattg ttcctttcaa aataccgcac
     6181 tgctaaaaca ctaataatgc acactaaaat ccgaatgtgc atagttaggg tttcagaccg
     6241 tgcggcaggt tgtaaacaaa ctttcagacg acatttcgga cgctgaaagc aattgcggat
     6301 tataaaggca accatcctta aaatccagta ttgatacaat aaaaaggccg tctgaaccat
     6361 atttctttca gacggccttc aaacttaaaa atcaatcctg agtcagcaaa gtcaatgctt
     6421 cccgatactt ctcgacagtt ttctgaatca catcggcagg cactttcggc gcaggggctt
     6481 ttttgttcca accgctttgt tccagccagt cgcggacgaa ttgtttgtca aaagacggcg
     6541 gattggtgcc gactttgtat tggtcggcag gccaaaaacg gctcgaatcg ggagtcaata
     6601 cctcatccat caacgtcagc gtaccttctt catccaaacc gaattcaaat ttggtatcgc
     6661 aaatgatgat gccgcgcgat ttagcatatt ccgccgcttc tgtataaagc tgaatcgctt
     6721 tggcgcgcac ttccgccgcc aattcttttc cgatactgcg ttcgcattct tcaaagctga
     6781 tgttttcatc gtgatcgccg actgcggctt tggttgaggg cgtaaaaatc acttcaggca
     6841 gttgttgcgc ttccctcata ccttcgggca gccgaatacc gcaaaccgag ccggtttttt
     6901 gataatcttt ccaaccgctg cctgccagat aaccgcgcac aatcgcctct actttcaccg
     6961 gagtgagctt tttagccacg acggcgcgtt tctctaaagc tttggcttcg ttttcaggca
     7021 aaacatcgta aaccgtttga ccggtaaagt ggttgggcat aatatgcgcc agttttttaa
     7081 accaaaaatt ggaaatctgc gtcagaatct cccctttgct cggaatcggg tcgtccaaaa
     7141 tcacatcaaa cgcggacagg cggtcggaag cgaccatcag catacgttta tcgtcgattt
     7201 catataaatc gcgcactttt ccagaataga tctttaccaa accaatctca ctcatttcgc
     7261 ccccccctga aaatatcttg aaaataccga cccgacaccc gacaggtttg aatcgcaaac
     7321 cgatattcta gccgaagtcg gcgcaaaaca atacccatgg cacaaaaagc caacccgtca
     7381 accgtcggca aagttttggc actataatac cgacagcaag tcctacaata cacttttacc
     7441 aaaggaaata cctcatgaga atcctattga caggctcgaa aagccaactg gcacgctgcc
     7501 tgcgcgaccg tcttccggaa gactgggaaa ccattgcgac ggattccgca tccctagaca
     7561 ttaccgatgc cgatgccgtc tgcaacatgg tcaaaagttt ccaacccgac gccattgtca
     7621 acacggctgc ctatactgcc gtcgacaaag cggaaggcga tgcggcagcg gcatttgccg
     7681 tcaatgcttc cgccgtttac aaccttgcct tggcagcaca tcgcgcccat gcccgattca
     7741 tccacatctc aaccgactat atctttgacg gaaaagggaa aagaccctac caggaaagcg
     7801 actttaccaa cccttccaat gtatacggac aatccaaaac cgcaggcgag ctgctcgcac
     7861 tgtctgccaa tcccgacagc cttatcctgc ggacttcttg gctgtttagc gaatacggga
     7921 acaactttgt ccgcacgatg ctgaaccttg cacgggaacg ttccccgctg tccgccgtcc
     7981 acaaccaaat cggctgcccg acctatgccg gcgatttagc tgccgccatc atccgccttt
     8041 tgcagcaaac gtcgcccgtg cgcggcattt accactacgc cggcggcaaa tccgtatcct
     8101 ggtacgaatt tgtccaacat attttccaag cggcatcgca acagcagaca tccttcccca
     8161 ttcccgaatt gactgccgtt tcagacaagg aatatccgac cgccgccccc aggcccgcat
     8221 acagcatttt ggactgccgc aaaatcgaaa acgacttcgg catcaaaccg tcagactggc
     8281 aaaaagccct tgcgcaggtc gtttccaagc tgctctgatg ccgcccgcct ctccgtttcc
     8341 gccgtcaagc accgccttgg cggtttt
//
LOCUS       DAXHRI010000083         8309 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXHRI010000083 DAXHRI010000000
VERSION     DAXHRI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.083"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(190..750)
                     /gene="sodC"
                     /locus_tag="ACFM75_001350"
     CDS             complement(190..750)
                     /gene="sodC"
                     /locus_tag="ACFM75_001350"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC6255888.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1167..2207)
                     /gene="mutY"
                     /locus_tag="ACFM75_001351"
     CDS             complement(1167..2207)
                     /gene="mutY"
                     /locus_tag="ACFM75_001351"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC6255889.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQILAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVGQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENALWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2370..3411
                     /locus_tag="ACFM75_001352"
                     /pseudo
     CDS             2370..3411
                     /locus_tag="ACFM75_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3423..3653)
                     /locus_tag="ACFM75_001353"
     CDS             complement(3423..3653)
                     /locus_tag="ACFM75_001353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255890.1"
                     /translation="MPSEKPFPFQTAFCRLRAQSAYTAALIRHTAPKMPIRLPHNKNN
                     GQFIRQQPDSPYYAAEKMPSETHSDGIRLKNN"
     gene            complement(3668..4306)
                     /locus_tag="ACFM75_001354"
     CDS             complement(3668..4306)
                     /locus_tag="ACFM75_001354"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC6255891.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     RNSSIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4487..6322)
                     /gene="edd"
                     /locus_tag="ACFM75_001355"
     CDS             complement(4487..6322)
                     /gene="edd"
                     /locus_tag="ACFM75_001355"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC6255892.1"
                     /translation="MNPTPIHPKLAEITERIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGGLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NNTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGRGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDPIRFDSVTGELNVLIDEAE
                     WNAREVEHIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            6805..8250
                     /gene="zwf"
                     /locus_tag="ACFM75_001356"
     CDS             6805..8250
                     /gene="zwf"
                     /locus_tag="ACFM75_001356"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC6255893.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMTGKVAEIVLNFKDLSSHIFESSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVEI
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT         1946 a         1956 c         2277 g         2130 t
ORIGIN      
        1 aatagtacgg aatcgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc
       61 taaggcgagc caacgttgta ccggtttaaa tttaattcac tatactgctg aagtcgtgaa
      121 ttaaatgcta aatatctagc aaaaaagtgc ggtcaaaatt taccgcactt ttcgtttcga
      181 acaatcgaat tatttaatca cgccacatgc catacgtggg ccgccaccgc caagtggagc
      241 tggatgatcg gagtgattat caccacccgt gtggatcata atagagtgac cgcgaacatc
      301 atctaaatgt ttaagacgtg gtgctaaaac aggatttgtt gctgtgccat catgcaatac
      361 agttaatgca ggtaaatcac ctaagtgtgc atcatcttgc catgggtaac catgttgttt
      421 tgcaccttta ggatcccagt gaccgcctgc gcctaaacca gctgtcaatt taccttcttt
      481 ttcttttggc tcacagcttg ggttttcatg gatgtggaaa ccatgtaagc cttcgcttaa
      541 tccttgtaaa tcaggggtaa acacaagacc atagttagat tcagtaatag tcactgtacc
      601 cacatctttg ttaccgttta ctggatcaag ttgttgcact ttcacttcaa tagaagcacc
      661 tttaggtatc gtattatgct cgtgtgcttg cgcaacacta actgaacata ctgcactaac
      721 cgctagtgct aataaggttt tcatattcat tgcaatctcc tttattgcag taaaaactcg
      781 cgactatttt agtcaatttt gttttaaaag ccgataaatt tttacgaaaa ttttacatta
      841 attgttattt gcagaaaatt agaacccgtt atcacgtctt ggataaatgc caaaaccatt
      901 gtgattccga gaattaaaaa gaaaaatttc ggaatcctca tccttttctg aaacctgatt
      961 gcggtatcca tggctttgtc tttgtcattt aagtttgtgt tcatgttttt gaccttcttt
     1021 gctttttaaa tcctttttaa attgccgact tcttccgttc caaatgtccc atttgaacat
     1081 tatcccgccg cctaaaatac gacccaaatt catcggttgt tcttgctgcg atacctgcaa
     1141 acaacagttc aatgggttta ttttgtttat tctaacctat taccgtttaa ataaatttcc
     1201 aaaggcttgg gcaggccgta atctttcaaa tgcgccggct ttatccaaat gccgtctgaa
     1261 gggctttcag acggcatttg tccttcaaac ggcgtaatca tcagcagccg gtgcgtcaga
     1321 cggtgggtca gggcggtttg ttcgtccata tctgccatgg tcagggagaa tttggcggca
     1381 aagtcggaaa gcccgttcaa actttcaaaa cacggcacgc aatacagccc gccccaaatg
     1441 cctttggcgg ggcgtttttc cagcaaaatc gcgccgtccc ggttgcggac aatcagccag
     1501 taaagcggca gggtcggtac ttcggcggcg gtttttttgc gcggcagctc ggcggtgcgg
     1561 ttttgctttt tcgcttcgca gatgtccgcc atcgggcatt ggtggcacaa gggtttcgtc
     1621 cgtttgcaca cggtcgcgcc caaatccatc aaaccttgtg tataggcagg catatcggcg
     1681 ttttcagacg gcagcaggct ttcggcaagt gtccagagcg cgttttcaaa ttttttgtct
     1741 tgcggattgc cgtcttgggc aaagacacgg cagagtacgc gtttgacgtt gccgtccaaa
     1801 atggtctcgc ggcggttgaa ggcaaaggcg caaatggcgg cggcggtgct tctgcctacg
     1861 ccgcagaggg tttccaagtc tttacgctcc gacggaaacg tgccgccgaa ttgtccgacg
     1921 acttgttgcg cggctttgtg cagattgcgc gcgcggctgt aatagcccaa gcctgcccac
     1981 aaggataaaa cctcgtcttg cggcgcggcg gcaagtattt gaacagtcgg gaatttttct
     2041 aagaaacgcg gatagtagtc caacacggtg gcgacctgcg tttgctggag catgatttcg
     2101 gaaagccaga cgcaataagg gtttttgacc tgccaaggga ggtggtggcg accgtgttgt
     2161 ttttgccagc ggatgagccg ttcggagaag gggatgggtg tgttcattaa tatcaatcgg
     2221 tggttttatt tatatttaaa acagtatgtt attgtataac attgtgaaaa taattcttat
     2281 ttacttattt tttgtagggg catataactc atataaagaa actttattgc ggtattgaaa
     2341 ttatttatca acaagcaagg agtatcagca tgaaagcaat ggtttatcac ggcgcaaacg
     2401 acatccgttt tgaagaaaaa ccccgtccgc agattatcga tccgaccgat gcggtggtga
     2461 aaatcgtcaa aaccacgatt tgcggtaccg atttgggtat ttggaaaggc aaaaaaaccc
     2521 gaagtcgccg acggccgtat tctcggtcat gaaggcatcg gtattgtaga agaagtcggc
     2581 gaggctgtaa aaaacatcaa agtcggcgat aaagtcatta tttcatgcgt cagcaaatgc
     2641 tgcacttgcg acaactgcaa aatccaactc tattcacact gccgcaacgg cggctggatt
     2701 ttgggctaca tgattgacgg cacgcaagcc gaatatgtcc gcacgcctta tgccgacaac
     2761 agcctcgtgc cgctgcccga caacgtcaac gaagaagtcg ccctgttgtt gagcgacgct
     2821 ttgccgaccg cacacgaaat cggcgtgcaa tacggcgatg tcaaacccgg cgacaccgta
     2881 ttcatcgcag gtgccgctcc tgtcggtatg tccgccttgt tgaccgccca actgtatagc
     2941 cccgccgcca ttatcgtgtg cgatatggac gaaaaccgtt tgaaactggc gaaagagttg
     3001 ggcgcgaccc ataccgtcag ccccgcttcc ggtgatgtgg gcaaacaagt tgccgctatc
     3061 gtcggcgaag acggtgtgga ctgcgcgatt gaagcggtcg gcatccctgc aacttggaat
     3121 atgtgccaag acattgtgaa acccggcggc catatcgccg ttgtcggcgt acacgttcaa
     3181 tccgttgatt tcaaactgga aaaactgtgg attaaaaatc ttgccatcac taccggtttg
     3241 gtcaatgcca acaccaccga aatgctgatg aaggcaattt ccagcagctc agtggattac
     3301 accaaaatgt tgacccacca tttcaaattc agcgaattgg aaaaagccta cgacgtgttc
     3361 aaacacgccg ccgaaaatca agcgatgaaa gtggttttgg aagcggatta atcggcgttg
     3421 aatcagttgt tttttaagcg gatgccgtct gaatgcgttt cagacggcat tttttcagcc
     3481 gcgtagtatg ggctgtccgg ctgctgtcga atgaactgac cgttattttt attatgcgga
     3541 aggcggatgg gcattttagg ggcggtatgt ctgatcagtg ctgccgtgta tgccgactgt
     3601 gccctcaatc gacaaaatgc cgtctgaaac gggaaaggtt tttcagacgg catttttacg
     3661 atgcggttca ggctttgggt ttcaatgccg ccgcttcttt ggcgaggcgg gtgatggtgt
     3721 cccagtcttt gtttttcacg gcttctttcg gtgtcagcca agagccgccg acgcacagga
     3781 cgttgggcag tgccaggtat tcgggggcag ttgccaggct gatgccgccg gttgggcaga
     3841 agcgaacatc ggcgtaaggg ccgtaaaggg ctttgagcat ggctttgccg ccgacgactt
     3901 cggcggggaa gagtttgagg gtgtcgatgc cgtgttccaa agccagttgg atttcgcccg
     3961 gggtggcaac accgggaatc agggggatgc tgctgttgcg gccggctttg gcgagggatt
     4021 catgcagccc cgggctgatg gcgaaaaccg cgcctgcgtc ttcgacggct ttgagctgtt
     4081 cagggttggt taccgtacct gcgccgacga tggcgttggg cacttctttg gcaatcaggc
     4141 ggatggcatc gaggccgaca ggggtgcgca gggtgatttc gagggtgggg atgccgcctt
     4201 cgacaagggc gtgggacaaa tcgacggcgg tgcttaagtc gtcaatcgcc attaccggca
     4261 caactgcgcc ggcggtcaaa atttcgcggg gggtcagttt ggacatttcg gttctccggg
     4321 tggaatgggg tattttatta agatgggaca ggttgtaggt cggattcaag aaaccgatat
     4381 cttggacggc tgtgtcggat tcgagaatcc aacctacgtc tatcttaaaa cgcttgaata
     4441 atttttcaga cggccttcgg tctgaaaagg ccgtctgaaa cgcgcatcag gcaaattcgc
     4501 cgccgaaact catggcaccg gtttccgcgc tgctggtcat gctgcggaag ttggcgaaga
     4561 gttcgcggcc gcagccttgt tggttcgcgc ccaagtcgat gtgttcgact tcgcgggcat
     4621 tccattcggc ttcgtcaatc aggacgttga gttcgccggt cacggagtcg aagcggatcg
     4681 ggtcgccggt acggattttg gcgatgttgc cgcccatcag ggcttcgggg gtcatgtgga
     4741 tggatgcggg aactttgccg gacgcgccgg acatacggcc gtcggtcaac agcgccactt
     4801 tgaagccgcg gtcttgcagg atgcctagag gcggggtcag tttgtgcaat tcgggcatac
     4861 cgttggcgcg cgggccttgg tagcggacga cgcagacgaa atcgcgttcc aattcgccgc
     4921 gctcaaacgc agccaacact tcgcgttggt cgttgaacac gatggcaggc gcttcgataa
     4981 tgcggcagcc ttcgcgcacg gcggacactt taatcacgcc gcgaccgatg ttgcctttca
     5041 tcaggcgcag accgccgtct ggggagaacg ggttgtcggc tttgcgtagg atgtcgtcgt
     5101 tgccgctggt ttcgggggct tcgcgccatt cgagtttgcc gtcgatgagg aaaggctctt
     5161 tggtgtagtg gcgcataccg cgtccgacga cggtatcgac atcgtcgtgc aacaggcctg
     5221 cgtccagcaa ttcgcggata acgaaaggta ggccgccggc agcggtaaag tggtttacgt
     5281 cggctttgcc gttggggtac acacggatca gcaatgggat gatggaagaa atttcgtcga
     5341 agtcgtccca gttcaaaatc acgcctgcgg cacgcgccat agcgacgagg tgcatggtgt
     5401 ggttggtcga gccgccggtc gccatcagac cgatcagggc gttgataaag gatttttcgg
     5461 tcaacatttc gcccaaaggt ttgatggtgt tgtttttaat gccgcgcgcg aggtgtccgg
     5521 ctgcgtagcg ggtcagggct tcgcgcaggt cggtgtaagg atggacgaat gcggcggcag
     5581 gcaggtgtac gcccatcatt tccatcatca tttggttgga gtttgccgtg ccgtagaagg
     5641 tacaagtacc cgggctgtgg taagaaccca tttcgctttt taaaagttcg tcgcgtccga
     5701 ccttgccttc ggcgaaaagc tggcgggtgc gggctttttc tttgttgccg atgccgctgg
     5761 acattgggcc tgccggaacg aagatgcccg gaatatggcc gaaagacaac gcaccgatca
     5821 tcaaacccgg cacgattttg tcgcacacac ccataaacag gccgccgtca aacatttggt
     5881 gggacagccc gacggcggta ctcatcgcaa tcacgtcgcg ggaaaacagc gacaattcca
     5941 taccggcgta gccttgcgtg atgccgtcgc acatagcggg cgtgccgccg gcgacttgtg
     6001 cggtcgcgcc gtttttctgc gcttcgtctt tgatttggtc ggggaagtct ttaaacggct
     6061 ggtgtgcgga aaccatatcg ttgtaggcgg tgatgatgcc taagttggga acggtttcct
     6121 gaagcatttc gattttgatg cttttgggca tggcggcgta gccgtgcgcc aaattgctgc
     6181 agccgagctg gttgcgctct aagcgtccca tctgtttggc actacggatt ttcgccagat
     6241 atttttcacg cgtcgggcgg ctgcgctcga tgatgcgctc ggtaatctcg gcgagtttag
     6301 ggtgaatagg agtggggttc atgtgctgtc tcctggcaaa acgatatgat agggattttg
     6361 tttgcgcgtt tctgattttt gtattgggtg ttttttcaga cggcctggag ctgtgggaat
     6421 aaataatgag tgccgtctga aatgttgaag atttctcttc gaagcgtctg cgtattatag
     6481 tacaaattct gtaattttgt tacaaaataa ttcgtaaagt ggaagaaatt tgatttaaat
     6541 cgaggtgtgg tcaattcgta aaatggtggg ctacaaaagt catcaaactt ttatgttaaa
     6601 tcaaacaatt tgtaaaaatg agaatagaca aaatctgtag ccatgtgtag tattactact
     6661 cactagcccg cttgtttgaa attttggtgc aggcgattgg ttttttactg cttattgctt
     6721 aaaggctttt gattttaatt gatttttatg gatgacgaca gtccaaaagt tttgaagcaa
     6781 gcttcctcaa cgagagatga aacgatgagt acacagacaa attttgattt ggtgttgttc
     6841 ggtgcgaccg gcgatttggc aatgcgcaaa cttttgccct gcctgtatca ggcgcacgtg
     6901 gccggtttgc ttcatcccga agggcgtatt ttgggggtca gccgcagcga gttggatacc
     6961 gaaggttttc tggcgaaggt ggaaaccagt tccaaaatcc atgtgaaaga aaatttttcc
     7021 gatgaggctt gggcatcgtt tgtcgaacgt ttcgcatatc tcaaagtcga tgttacgcag
     7081 ccggacgatt ttgccgcttt gggcgatttg gtcaaagcgc gcaaagaaac cgacaatgtc
     7141 gtcatctatc tttccaccgc gccgaaattc ttcgcgcaaa catgtgaaaa ccttgccaaa
     7201 atcggtttga acgccgacaa cgtgcgtgtg gttttggaaa aaccgttggg tacggatttg
     7261 gcttcttccc agcaaatcaa taccgatgtt gcgcgttatt tcaaagaagg gcaaatttac
     7321 cgcatcgacc actaccttgg caaagaaagc ctgcaaaacc tgcttgcgct gcgttttgcc
     7381 aatgtaatgt tcgagccgct gtggaacaat aaatacatcg aaagcgtgca gttgaccatc
     7441 gccgaacagc tcggtgtgga agagcgcggc gagttttacg acattaccgg cgcgttgcgc
     7501 gatatggtgc aaaaccattt gatgcagatg ctgtgcatga ctgcgatgga agcccccgcc
     7561 agcttggatg ccgacgcggt gcgcgatgaa aaagtcaaag tcatcaagtc attgaagccg
     7621 ctgaccgtcg aatctgtcaa tgaaaacgtc gtgcgcggac aatataccgc cgccaaaggc
     7681 atgaacggct atcttgaaga aatcaacgtt ccgcaagaca gctttaccga aacctacgtc
     7741 gccattaaag ccgaaatcga aaacgaacgc tggaagggcg ttcccttcta cctgcgtacc
     7801 ggcaaacgca tgacgggtaa agtggcggaa atcgttttga acttcaaaga tttgagcagc
     7861 catatttttg aaagcagccg caccgcgccc aatcggctcg ttatcgagtt gcaaccatat
     7921 gaatccgtgc gcctctatac gcagatgaaa accccgggtg caggcaataa ggtcgaaatc
     7981 gtgccgctgg caaccgattt gggcaaagca ttggaaggcc gccgcgcgga agcttacgag
     8041 cgcctgctgt tggatgtgat taacggcaaa ctcgctttgt ttaaccgccg agacgaactc
     8101 gaagccgcat gggaatatgt gatgccgatt ttggaaaact ggacaaataa caccacgccg
     8161 ccgcacggct acggcgcaca ctcgtggggg cctgaagccg cgcgcgaact attggcgcgc
     8221 gacggacaca agtggcacga agagcagtaa tacaataatg cgttcagacg gcatggggtt
     8281 tgaaatgccg tctgaacgta agtaaagca
//
LOCUS       DAXHRI010000084         8241 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXHRI010000084 DAXHRI010000000
VERSION     DAXHRI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.084"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..114
                     /locus_tag="ACFM75_001357"
     CDS             <1..114
                     /locus_tag="ACFM75_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFC6255894.1"
                     /translation="SLIWNDPTVGIDWPLQGEPNLSPKDLAGKVLAEAVTF"
     gene            138..2252
                     /locus_tag="ACFM75_001358"
     CDS             138..2252
                     /locus_tag="ACFM75_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC6255895.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HIYKDGKGAEAVVGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPKIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGCFAKKLGKIKQLYRSFK"
     gene            2389..3648
                     /locus_tag="ACFM75_001359"
     CDS             2389..3648
                     /locus_tag="ACFM75_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC6255896.1"
                     /translation="MKQTVLQNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLASFAAHAP
                     ADTNLIIKHHPMDRGFIDYSRDIKRFIKKHPKLKGRITYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAHYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSDSSTPAT"
     gene            3824..5230
                     /locus_tag="ACFM75_001360"
     CDS             3824..5230
                     /locus_tag="ACFM75_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6255897.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5448..6662
                     /gene="gltS"
                     /locus_tag="ACFM75_001361"
     CDS             5448..6662
                     /gene="gltS"
                     /locus_tag="ACFM75_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC6255898.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6761..7777
                     /locus_tag="ACFM75_001362"
     CDS             6761..7777
                     /locus_tag="ACFM75_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6255899.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAHEDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7788..7985
                     /locus_tag="ACFM75_001363"
     CDS             7788..7985
                     /locus_tag="ACFM75_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255900.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            8003..8152
                     /locus_tag="ACFM75_001364"
     CDS             8003..8152
                     /locus_tag="ACFM75_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255901.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAVFSCVSYADVSTHKTAHKAL
                     PYVPS"
BASE COUNT         2081 a         2444 c         1910 g         1806 t
ORIGIN      
        1 tcactaattt ggaatgatcc gaccgtcggc atcgactggc cgctacaagg cgagccgaac
       61 ctgtcgccta aagacttggc cggcaaagta ttggctgaag cggtcacgtt ttaagactat
      121 tcaggccgtc tgaaagaatg ttcctctttt cagacggcct acaatctatt aataacaata
      181 atcgacgaaa acgcattgtg aaaaacgcct acatcccctc tcgcggcatc cgcaaaatcc
      241 cccatctctc caccctattg cccgagtttc atatctacaa agacggaaaa ggagcagagg
      301 ctgttgtcgg ctggggtttg cgcccgacga cacacaaagc gcgtgctttt gccaccgaac
      361 accagcttcc ctttattgct ttggaagacg gctttttacg ctcgctcgga ctgggcgtct
      421 ccggttatcc cccctactcc atcgtctatg acgacatcgg catctactac gacactaccc
      481 gcccttctcg tttggaacaa ctgattcttg ccgccgatac catgccgtct gaaaccttgg
      541 ctcaggcgca acagacgatg gatttcatcc tgcaacacca cctgtccaaa tacaaccacg
      601 cgcccgaact ttcagacgat catcctttac gctccccatc caagcctgaa actgtcctca
      661 ttatcgacca aaccttcggc gatatggcca tccaatatgg cggcgcagac gcctctacgt
      721 ttgaactgat gtttcagacg gccttgaatg aaaacccgca agccgatatc tgggtaaaaa
      781 cccatcccga tgttttgtgc ggcaaaaaac aaggctatct gacccaactg gcgcagcaac
      841 accgcgtcca tcttctggca gaagacatca acccgatttc tttgttgcaa aacgtcgata
      901 aagtttattg cgttacctcg caaatgggtt ttgaggcact tttgtgcggc aagccgctga
      961 ccaccttcgg cctgccgtgg tatgccggat ggggtgtaag cgacgaccgc catcctgaaa
     1021 tcaaccgcct tgttcaaacc caacgccgcg ccacccgcaa cttgctgcag ctcttcgccg
     1081 cagcctatct gcaatacagc cgctacctca accccaatac cggcgaagca ggcagcctct
     1141 ttgatgtcat cgactatctg gcgacggtca aacgtaaaaa cgacaaattg cgtggcgagt
     1201 tatattgcgt cggtatgtct ttgtggaaac gcgcggttgc caaaccgttc tttaacgtac
     1261 cctcttgccg tctgaaattt atctcttcca cccaaaaact ggcaagggtc aaactgtccg
     1321 acgatgcacg catcctggct tggggcaacg gcaaagaggc catcgtccgc tttgccgaac
     1381 aacaccacat ccccctgctg cgcatggaag acggctttat ccgctcggtc ggactcggct
     1441 ccaacttagt gccgccgctg tcgctcgtta ccgacgatat gagcatttat ttcaatgccg
     1501 aaaccccgtc ccgtcttgaa tacatcctac aaaaccaaaa cttcgacgat caagactttc
     1561 agacggcctt gaagctgcaa aaaatgctga ccgaaaacca catcagtaaa tacaacgtcg
     1621 gcagctcaga cttcaccgcc ccgtcaaccg acaaaaccgt gatcctcgtt cccggccagg
     1681 ttgaagatga tgcgtctatc cgctacggtt cgcccaaaat ctatcgcaat ctggatttgc
     1741 tccgtaccgt acgcgaacga aaccccaatg cctatatcat ctacaaaccg catcccgatg
     1801 tagtcagcgg taaccgcatc ggccatattt cccctgaaga tgctgcacga tatgccgacc
     1861 aaaccgccga acaagccgac atcctgacct gtctccaata cgcagacgaa atacatacca
     1921 tgacttcgct gaccggtttt gaagccttgt tgcgcggcaa aaaagtcagc tgctacggcc
     1981 tgccttttta cgcaggctgg gggcttaccc aagatctgct ccccatcccg cgccgtagcc
     2041 gcagacttga gctttggcag ctgattgccg gcacgctcat ccactatccc gactacatcc
     2101 accccgaaac ccatcaggcc ataaatgcag aaaccgcagc ccaaatcctg atacgacaaa
     2161 aaaatatgca aaaaaacaac aacggattac atcgcgggtg ctttgccaaa aaattaggta
     2221 aaatcaaaca actatatcga tctttcaaat aaataccatc aaagttaacg atgcgtcata
     2281 aacttgcctc tattgcggca tcattgcctt tgcatcgtta attctcttgg cgtatgcttg
     2341 aaagttcaac ctaaaactat tacataaaaa acaaaaccac attgcaacat gaaacagacc
     2401 gtcctccaaa acaacctgca aaacctgctt gaaagcgcag aaaatatcct gctgcttcaa
     2461 ggccctgtcg gcgatttttt tctgcgcctt gccgactggc tgactgcaaa cggcaaaacc
     2521 gtacataaat tcaactttaa tgcaggcgac gactattttt atccgcccac tcaagcgcat
     2581 accgttgttt ttaacgacaa ctacgatgcc tttcctgagt ttttgcaaga atacatcact
     2641 caacatcaca tccaggccgt tgtctgcttt ggcgacacac gcccttatca cgtcattgca
     2701 aaacgcattg caaacgaaaa ccaagccagt ttctgggcgt ttgaagaagg ctatttccgc
     2761 ccctactaca tcaccttaga aaaagacggc gtcaacgcat tttccccgtt gccgcgccgt
     2821 gccgactttt ttcttgaaca attccctaag cttgcccagc aagaatataa agcgccaacg
     2881 ccggtacacg gcggttttac gcccatggca aaaaacgcta tccgttacta tatcgagttg
     2941 ttccgcaatc cacgcaaata ccccgactac atccaccacc gcgcacccaa tgccggccat
     3001 tacctcaaac catggtcgct ctccatcctc aagcgtttga actactatat tgaagacatc
     3061 caaatcgcca aacgtgtgga agcaggcaaa tacggcaagt tttttattgt tcccttacag
     3121 gtattcaacg acagccaagt ccgtatccat tgcgactttc ccagcgtccg cagcttcctg
     3181 ctccatgttt tggcctcctt tgccgcgcac gcgcctgccg ataccaacct catcatcaag
     3241 catcatccga tggaccgcgg ctttatcgac tactcgcgcg acatcaaacg ctttatcaaa
     3301 aaacacccca aactcaaagg ccgtatcact tacgtccatg atgtccccct gcccgttttc
     3361 ctgcgccacg gcctcggcat ggttaccatc aacagcacca gcggcctgtc cggcttgatc
     3421 cacaacatgc cagtcaaagt cctcggccgc gcccattacg acattcccgg cataaccgac
     3481 caaaatacct tggcagaatt ttggaatcat ccgacaccgc ctgacaaaga gctgttccat
     3541 gcctaccgaa tgtaccacct caacgtgacc caaattaacg gcaacttcta cagtcaggtc
     3601 ttttttccta acaaaaaaac ctccgactct tccactccag caacctgact tagcaaaaat
     3661 aattcaggcc gtctgaaaac atttcagacg gcctgaaaca atcaatacct tagctactgc
     3721 catgtaaata aaacacaaaa acctattttt acaaagccct caaccggtat cgccgcccat
     3781 atgccgcagc atccgtcttc cactttatat ccgcccgcaa accatgaccg ccgctcctga
     3841 tatcctctac cggcaagccg ccgccctttt ggaacaatcc aataccgccc aagccctgcc
     3901 cctgttgcaa caggcggcag agcaaggtta tgcggaagct gctttcgtat tgggcaacca
     3961 tctgctgcaa aacggccaac cggagcaggc actttcatgg ttggaagccg ccgcggccca
     4021 acgccatccc aaagcactct tctccctgct gcaacaacgc gaacacaacg gcaccccgac
     4081 cggacagctt ctcaacgact atgcctggct gggtgagcag gggcactcag aagcccaatt
     4141 aatcctcatg cgttaccacg cgcaacgcaa cgatccacaa tcgctctact gggcggaact
     4201 tgctgccgcc cgatatgccg cacctgcgta ttaccatctg gcacgccatc atcaacgcca
     4261 aggcgacgtt gaaacagcca tcgaacaata cgaaaaagcg gcagcactcg gcgtaactgc
     4321 cgcctgctgg caacttggtc aaatctactt ctacggtaca ggtgtcagcc ccaaccacgc
     4381 acaagccgaa cactatctcg aaccagccgc acaagccggc cacatcgccg cacaaacgct
     4441 gctggctgac cttcttgccg cccaacgcaa acctgaagcc ttggaatggt atcgtcgtgc
     4501 cgccgataag gaacaagcgg aagcacagtc taagctggcc caatacgccc tgaccggcga
     4561 actttccgaa cgcgatccgt tccaagcggc acgatatgcc aaagccgctg ccgagaaaaa
     4621 ccatcctgaa gccctgaaaa tcatgggcga cctctaccgc tacggtctcg gtatcaaagc
     4681 cgacaaccat atcgcgcaag attactacca ccgtgccgcc gcgctgggtt ctgccgccgc
     4741 agcacaaaaa ctcatcagcg acgccgcgct gtaccatccg caacaatacg aacaaatcaa
     4801 aactgccgcc ctgcaacaac aacaaaccga aaccatctac cgtttggcgg aagcacaagc
     4861 ctgcgccatc ggccgtcccg ccgactacaa tgccgcgcga aaaaattaca tggaagctgc
     4921 cgggttccac cataaaaacg cagcggcagc cttaggccgc atctaccatt acggcctcgg
     4981 tacggcgcaa gatcctcggg cggctgcaca ctggtacgcc attgctgccg aacaaaacca
     5041 cccttccgcc caataccacc tcgcctgttt ttactatcac gggcaaggtg tcggctgtca
     5101 tgttccgacc gcctgctact ggctgcaggc cgccatcggc aacggccaca cttcggccga
     5161 atcattaata tccctattag aacaatggcg acgcgaagca caccatgcca tcggacaaaa
     5221 ggccgtctga aaagatttac actcgcattt tttgacaatc tttaactatt cccctaatat
     5281 ttgccagtta tttttcacgg acacgccatt gttttcattt ctttctgaaa acaccttgtc
     5341 cgcgcatcaa taccatgaca ctcggcggat aacgccaagc gttgaaacac actacatccg
     5401 gaacaaaaac ggatgctcgg aaaaatattt ctaggaggtg aaacaacatg gaatgggaat
     5461 tcaacagtta ttacacactg attgccgcca cgctcgtgtt gctggttggt aaatttctgg
     5521 ttcaaaaaat caaattctta cgagacttca atattcccga gccggtcgct ggcggtttgg
     5581 ttgccgcgat tatcctgttt gcgctgcacg aggcgtacgg cgtgagcttc aaatttgaga
     5641 aaccgctgca aaatgcgttt atgctgattt tcttcacgtc catcggcttg agcgcggatt
     5701 tttcccgttt gaaggcgggc ggtttgccgc tagtggtttt taccgcgatt gtgggcggat
     5761 ttatcttggt gcaaaacttt gtcggggtcg gactggctac ggctttgggt ttggacccgc
     5821 tcatcggtct gattaccggt tcggtgtcgc tgacgggcgg acacggtacg tcaggtgcgt
     5881 ggggacctaa ttttgaaacg caatacggct tggtcggcgc aaccggtttg ggtattgcat
     5941 cggctacttt cgggctggtg ttcggcggcc tgatcggcgg gccggttgcg cgccgcctga
     6001 tcaacaaaat gggccgcaaa ccggttgaaa acaaaaaaca ggatcaggac gacaacgcgg
     6061 acgacgtgtt cgagcaggca aaacgcaccc gcctgattac ggcggaatct gccgttgaaa
     6121 cgcttgccat gtttgccgcg tgtttggcgt ttgccgagat tatggacggc ttcgacaaag
     6181 aatacctgtt cgacctgccc aaattcgtgt ggtgtctgtt tggcggcgtg gtcatccgca
     6241 acatcctcac tgccgcattc aaggtcaata tgttcgaccg cgccatcgat gtgttcggca
     6301 atgcttcgct ttcgcttttc ttggcaatgg cgttgctgaa tttgaaactg tgggagctga
     6361 ccggtttggc ggggcctgta accgtgattc ttgccgtaca aaccgtggtg atggttttgt
     6421 acgcgacttt tgttacctat gtctttatgg ggcgcgacta tgatgcggca gtattggctg
     6481 ccggccattg cggtttcggc ttgggtgcaa cgccgacggc ggtggcaaat atgcagtccg
     6541 tcacgcatac tttcggcgcg tcgcataagg cgtttttgat tgtgcctatg gtcggcgcgt
     6601 tcttcgtcga tttgattaat gccgcgattc tcaccggttt tgtgaatttc tttaaaggct
     6661 gattttccgc ctttccgaca aagcacctgc aaggtttacc gcctgcaggt gcttttgcta
     6721 tgatagccgc tatcggtctg caccgtttgg aaggaacatc atgtatcgga aactcattgc
     6781 gctgccgttt gccctgctgc ttgccgcttg cggcagggaa gaaccgccca aggcattgga
     6841 atgcgccaac cccgccgtgt tgcaaggcat acgcggcaat attcaggaaa cgctcacgca
     6901 ggaagcgcgt tctttcgcgc acgaagacgg caggcagttt gtcgatgccg acaaaattat
     6961 cgccgccgcc tacggtttgg cgttttcttt ggaacacgct tcggaaacgc aggaaggcgg
     7021 gcgcacgttc tgtatcgccg atttgaacat taccgtgccg tctgaaacgc ttgccgatgc
     7081 caaggcaaac agccccctgc tgtacgggga aaccgctttg tcggatattg tgcggcagaa
     7141 gacgggcggc aatgtcgagt ttaaagacgg cgtattgacg gcagccgtcc gcttcctacc
     7201 cgtcaaagac ggtcagacgg catttgtcga caacacggtc ggtatggcgg cgcaaacgct
     7261 gtctgccgcg ttgctgcctt acggcgtgaa gagcatcgtg atgatagacg gcaaggcggt
     7321 gaaaaaagaa gacgcggtca ggattttgag cggaaaagcc cgtgaagaag aaccgtccaa
     7381 acccacgccc gaagacattt tggaacacaa tgccgccggc ggcgatgcgg gcgtacccca
     7441 agccgcagaa ggcgcgcccg aaccggaaat cctgcatccc gacgacggcg agcgtgccga
     7501 taccgttacc gtatcacggg gcgaagtgga agaggcgcgc gtacaaaacc agcgtgcgga
     7561 atccgaaatt accaaacttt ggggaggact cgataccgac gtgcaaaaag agttggtcgg
     7621 cgaacaacgc aagtgggcgc aggaaaaaat cagcaactgc cgacaagccg ccgcgcaggc
     7681 agaccggcag gaatacgccg aatacctcaa gctgcaatgc gacacgcgga tgacgcgcga
     7741 acggatacag tatcttcgcg gctattccat cgattagggg caaaccgatg aataccgtcc
     7801 caaaaagcag gattcccgtc aaaccgctgc ccgaaaaaac cacagacgaa gccaaagtcg
     7861 aaaaatggcg gcagctcggt gcggaacacg gtttgtcggg cgaatgggca gttgccgcca
     7921 gattgggcga aaacggtttt accgaagaac agatggaaaa tatcgccaac ctgttcggca
     7981 gataaagaga aaattgacgg aaatgccgtc tgaaaccctg ttgtcggttt cagacggcat
     8041 tttgaccaat acggtacgca ggcgcaaaac agccgtcttt tcctgcgttt cctatgctga
     8101 tgtttcaaca cacaagacgg cacataaagc gttgccctat gtgccgtcct gattcggaag
     8161 gggttacgcc cctcccaaat atagtggatt aacaaaaacc agtacggcgt tgcctcgcct
     8221 tagctcaaag agaacgattc t
//
LOCUS       DAXHRI010000085         8147 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXHRI010000085 DAXHRI010000000
VERSION     DAXHRI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.085"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..1116)
                     /locus_tag="ACFM75_001365"
     CDS             complement(<1..1116)
                     /locus_tag="ACFM75_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6255902.1"
                     /translation="MADYYAITVKFAEQGTPLKQGGVSKTGHVWVELYSARDKKIYSF
                     GWSTGDSKTEGGFDNLVKDDRSNYINHPDTPISSITVPITKEQFDRAIAFPARAASGQ
                     VDGFTNIFGQTSYGILKNSCVDYGGKSIADIGLADKDFDGHPLKAPYYQYSDFLEQME
                     KHGESWILTERDGRRQFKWLGKPDPTRGWFDNGHECLKPWGNIFDSYRRGKYHVYDPL
                     ALDLDGDGIETVATKGFAGALFDHRNQGIRTATGWVSADDGLLVRDLNGNGIIDNGAE
                     LFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQSLRVWQDLNQDGISQANE
                     LRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKT"
     gene            complement(1143..1658)
                     /locus_tag="ACFM75_001366"
     CDS             complement(1143..1658)
                     /locus_tag="ACFM75_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255903.1"
                     /translation="MSAFRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKK
                     TKRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKK
                     LIIDELLTERSRKLGGGVFGAGGKYSMHIYDFYLPEGEYLFEISDNSEYIPLYDEINN
                     SIRIVVNARIQ"
     gene            complement(1918..2475)
                     /locus_tag="ACFM75_001367"
     CDS             complement(1918..2475)
                     /locus_tag="ACFM75_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpA"
                     /protein_id="HFC6255904.1"
                     /translation="MENNKAKSDDNSVSIEFIQDFITASTDVINLSYENFRKNFYTQM
                     STDSTNYAAKHESLGKSVQRELQKTQSQLRQVVRKMQSKYNINNKARVAEISLLRQMQ
                     SQFSRKDVNKNLGNGNTLAKQGSYTKKDGTTAQAGDSLLAADNLHSLLTDKMLSISHV
                     RENTISPFVLGCLKQITINVSHYLK"
     gene            complement(3013..3297)
                     /locus_tag="ACFM75_001368"
     CDS             complement(3013..3297)
                     /locus_tag="ACFM75_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255905.1"
                     /translation="MKLALKRSGKYNVYNPLALDLDGDGIETVAAKGFSGSLFDHTNN
                     GIRTATGWIAAYDGFPVRKLNSNGGIISTTDTIFQSLHTWLDHQPNDTCH"
     gene            complement(3294..3587)
                     /locus_tag="ACFM75_001369"
     CDS             complement(3294..3587)
                     /locus_tag="ACFM75_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC6255906.1"
                     /translation="MNIDDFTPSKIANFFADPDTYSNVLEEVSRFIYSLVPDDANPWK
                     GGEDYIGRGISEWGELLEKWYKQDFLPYLEKNGTNFRNLKIGCLNSLNGQESG"
     gene            complement(3925..4146)
                     /locus_tag="ACFM75_001370"
     CDS             complement(3925..4146)
                     /locus_tag="ACFM75_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HFC6255907.1"
                     /translation="MTSANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSNWKR"
     gene            complement(5005..6165)
                     /locus_tag="ACFM75_001371"
                     /pseudo
     CDS             complement(5005..6165)
                     /locus_tag="ACFM75_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FrpA/C"
     gene            complement(6152..6871)
                     /locus_tag="ACFM75_001372"
     CDS             complement(6152..6871)
                     /locus_tag="ACFM75_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255908.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            complement(7147..7581)
                     /locus_tag="ACFM75_001373"
     CDS             complement(7147..7581)
                     /locus_tag="ACFM75_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255909.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            7907..>8147
                     /locus_tag="ACFM75_001374"
     CDS             7907..>8147
                     /locus_tag="ACFM75_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6255910.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAVLFY
                     RKIRMVISHYLALAANEVFEGSVELDESYFGGRRKG"
BASE COUNT         2621 a         1540 c         1280 g         2706 t
ORIGIN      
        1 tgttttggtg tagctgcctt gctgagccaa agtgttaccg ttaccgagat ttttatttac
       61 atctttatag gcgagatcca aagattggat acccaattct tcaagggtac gcaattcatt
      121 agcttgggaa atgccgtcct gattgagatc ctgccataca cgcagggatt ggaatgcggc
      181 gtctgccgcg ttgatgatgt tgtcgccgtt tgaatccaat tcggccaaag ccgcgtagcc
      241 gtgtttggca aaagaaccgt ctgccagttt ggtgttgtcg ccgaagagtt ccgcaccgtt
      301 gtcgatgatg ccgttgccgt tcaaatcgcg cacaagcaga ccgtcatcgg cagaaaccca
      361 accggtggcg gtgcggatgc cttgattgcg gtggtcgaac aatgcacctg caaagccttt
      421 ggtggcaacg gtttctatac cgtcaccgtc caaatccagg gcaagggggt cgtagacatg
      481 atatttgcca cggcgataag aatcgaatat gttaccccaa ggtttcaagc attcgtgtcc
      541 gttatcaaac cagccgcgcg taggatctgg cttacctaac catttaaatt gtcgccttcc
      601 atctctttcg gtaagaatcc aactttctcc atgtttctcc atctgttcta aaaaatcact
      661 gtattggtaa tagggagcct tcagtggatg gccatcaaaa tctttatccg caagaccaat
      721 atctgcaata cttttccctc cataatcaac acagctattc ttaagtattc catatgaagt
      781 ttgaccaaat atattggtaa atccatctac ctgacctgat gcagctctag caggaaaagc
      841 tatagcacgg tcaaactgtt cttttgtaat cggtacagta attgagctga tgggggtatc
      901 tggatgatta atataattac tcctgtcatc tttaacaaga ttatcaaatc ctccttcagt
      961 cttagaatcc ccagtagacc atccaaaact atagattttt ttatctctag ccgaatacag
     1021 ttcaacccaa acgtgccccg tcttggatac acccccttgt ttcagtggcg taccctgctc
     1081 cgcaaatttt acagttatcg cataataatc agccataagt aaccccacat ttctagaaaa
     1141 atttactgaa ttcgtgcatt aactactatt cttatagaat tatttatttc atcgtaaagt
     1201 ggaatatatt cactattatc agaaatctca aataaatatt ccccttcagg caaataaaaa
     1261 tcataaatat gcatactata tttcccccca gctccaaata ctccgcctcc taattttcta
     1321 cttctctctg taagcaactc atctataata agttttttac catcttcttc cactttaaat
     1381 attttaaccg atatattata tggatagttt aacttatcat caatccacct ctccccctgt
     1441 tttggatcat aatgtataaa attagttcct aacgatattg cataaggttt accaattttc
     1501 tgtttgagtt caatagatct ttttgttttt ttaccaagag gaaagtctac cacaacaggt
     1561 tctgggctaa tagccatata aaaaatagtt tcaacaaaac tgcagctagc aatcaatagg
     1621 caagatatta ttaataatat ttttctaaat gcagacataa taaaaatctc caaaaaatcc
     1681 atatcagaat atactaatta tttgcataat taataaggaa gagaaaaatt caaaacggct
     1741 atgagatttc cgcctgaaaa taatgaaata aaagtccata gtaatgaata atagctttat
     1801 cagcccccct ttaatgcaaa catagagaat acttcccttg aagcactatc aatttctact
     1861 taactaaaac tctagctcta ttttaaaatt ttgtcaaaaa tagttaaata cttttattta
     1921 ttttaaataa tgagatacat ttatggttat ttgttttaga cagcctaaaa caaacgggga
     1981 aatcgtgttt tcccgaacat ggctaatgga tagcattttg tccgtgagga ggctgtgcag
     2041 gttgtcagca gccaacagcg aatcgcctgc ttgcgcggtt gtgccgtctt ttttggtgta
     2101 gctgccttgt ttagccaaag tgttgccgtt accaagattt ttgtttacat cttttcgaga
     2161 aaattggctt tgcatttgcc ttaacaaaga tatttctgct actcgtgctt tattatttat
     2221 attatattta ctctgcattt ttcttacaac ttgtctcaac tgactttgtg ttttttgtaa
     2281 ttcacgttgt accgattttc ctaaactttc atgtttggct gcataattgg tagaatcagt
     2341 tgacatttgt gtataaaaat ttttacgaaa attttcgtaa ctcagattaa ttacatccgt
     2401 acttgcagtt ataaaatctt gtataaattc aatagaaact gaattatcat cagatttggc
     2461 tttgttattt tccatattga gcctctctta atttgaaaaa attaacataa acatttaaaa
     2521 ataaaacaaa attatataat aaaaaaaata atgagaatat aaaggataaa aagagagtta
     2581 agttatttaa tcggacttgt tctctataaa tcataaggca ccaaataagc cataataaat
     2641 ttagtaaata aaaactgctt atacccaact ttcttttctt ggaacctatt attatttcaa
     2701 ctctggatga aaaaatagaa tttaaaaatt tttttctttg atatatcgtt aatatctccc
     2761 tagtttttct taaaaaaaat ataatcatat atatactaca caacattggt aataaataaa
     2821 ttaaaagcga aaggtaatta aattgataat tgtaaattat ataataaaca acatacgaag
     2881 aaaaaattat tatagatgat ttgctatacg aataaaaaaa tatcctacct agcccgaacc
     2941 ttcttataat ttgtactatg tgatcattca tttcaatatc catttaagct ttcaattaat
     3001 tttctatatt tttcaatggc atgtatcatt tggttgatga tcaagccatg tatgcaaaga
     3061 ttggaatatg gtatctgtcg tgctaataat gcccccgtta ctgtttaatt tgcgcacagg
     3121 aaaaccgtca tatgcagcaa tccagcccgt ggcggtgcgg atgccgttgt tggtgtgatc
     3181 aaataagctg cctgaaaagc ctttggcggc gacagtttct atgccgtcac cgtccaaatc
     3241 tagggcgagg ggattgtaaa cgttatattt gcctgaacgt ttgagagcta atttcaacca
     3301 ctctcttgcc cattcaggga attcaggcag ccaatcttca aatttcggaa attggtccca
     3361 ttcttttcaa gataagggag aaaatcttgt ttataccatt tttccagtaa ctctccccat
     3421 tcacttatcc ctcgtccaat ataatcttcg ccccctttcc aagggtttgc atcatcagga
     3481 actaaggaat atataaacct agatacttct tctaatacat tgctgtatgt atcaggatcc
     3541 gcaaaaaagt ttgctatctt acttggtgta aaatcatcta tattcattat agattcgcca
     3601 agcctctcgt ttccataatc cttatttaat tcaattcatg ctgtaaaaca gtagaggatt
     3661 ttagcattct agaattaagg acatcattta aaccagtttt taatttagtt ggtaagaatt
     3721 tgctttgagc catcgcctct gtttgttgtt ttatatattg taatccatca ccagcagcaa
     3781 tgaatatatc accaattgct tgttgaatat cattaggttt aaattttcct gtttctcgta
     3841 tatctcgaga tacatttctt aaatttaaaa aatgttatcg aaggatgcaa gtaaagaagc
     3901 agtatttgtt attttgttat tttgttatct cttccaatta ctgcttaaaa agctatctgc
     3961 agcttgaata gcattattaa cagtaatttc cattttattt ctattgttat ccacattata
     4021 gattaaatca gataaggatt gaatagaacc aataaatgaa tcccaagctt tatatcccaa
     4081 gagtggtgaa tagggtgtta atttcacatc tccaaaaccg ttaatattaa aatttgcaga
     4141 tgtcatatct gctttccttt caagtaaatt aaaaaaataa cgtgaggaat taaaattaag
     4201 aataaattaa ttatcgactc aatataatat gaatcaatta gctctcttat ggtaaatata
     4261 agaggtaaaa atattaaaaa taaactaata aagttcagaa aaatagtaaa tggaatattt
     4321 tcatatggaa aaattctaca aaatacaagt aatattccat gacatattag caaatgagta
     4381 aaatttatta taaaatatat aaatatttcc ccatcgtaag atatattcaa ataaagacat
     4441 ataaacaata tgaagtatat aaaatatatt ttttggcatc taaaaaataa acttttagca
     4501 atcatatata aatagcttgt agaataaaaa acgaaaatta ttaattatcg taggtgtgca
     4561 tttattccaa cacggtttca tgcagcttaa aaaccaaaaa ttctttctcc ctttatcctt
     4621 tcttaaaaat agcgttttca aattctgcaa cgacaggttt cattcaagta ggactgccgt
     4681 gattgcatca attgcttcgc ggcatggcat ccatatcaca caacaggaca tttattctcg
     4741 ggtaagtcca ccttcatcct cttccgccag caggaacagt tacaggctac ctaaaatatg
     4801 ctctgtgctc ggatagtaaa tatttccttt ctcactattg tttgtcattt aatcaacagt
     4861 ctactctttc tctaaaattt cgtcaataat agttaaatac ttttatttat tttaaataat
     4921 gatatacatt tatggttatt tgtttagaca gcctaaaaca aacggggaaa tcgtgttttc
     4981 ccgaacatgg ctaatggata gcattttgtt cgtgaagcgg ctgtgcagat tgtcggctgc
     5041 taaaagtaaa tcccccattt ttgcggttgt accgtctgtt ttggtatagc tgccttgctg
     5101 agccaaagtg ttaccgttac cgagattttt atttacatct ttataggcga gatccaaaga
     5161 ttgaataccc aattcttcaa gggtgcggag ttcgttggtt tgggaaatgc cgtcctggtt
     5221 gagattctgc catacacgca gggattggaa tgcggcgtct gccgcgttga tgatgttgtc
     5281 gccgtttgaa tccaattcgg ccaaagctgc gtagccgtgt ttggcaaaag aaccgtctgc
     5341 cagtttggta ttgtcgccga agagttccgc gccgttgtcg atgatgccgt tgccgttcaa
     5401 atcgcggacg agtaaaccgt catcggcaga aacccagcca gtggcggtgc ggatgccgtt
     5461 gccgttatgg tcgaagaggc tgcctgaaaa gcctttggcg gcgactgttt ctatgccgtc
     5521 gccgtctagg tctagggcga gggggtcgta gatgtggtat tggcctgaac ggtttctgtc
     5581 caattctttc cacagattcc agccgtcggg cagccagtcg tatacccaac gagaccagtc
     5641 ttcaaatagc tcatacccaa aacctttcca gttgtcaaaa ttataaaatt cctctgtaga
     5701 tatcgtacca gtatctcctg tggcaatagc cgttgctgca attatatctc cagcaattgc
     5761 taatggaatc ccccaactct gcatatttcc attcttctct attaatgtac ttcctaaccc
     5821 tcccaatgaa tataaatccg ctaaaatatt ttgaaaacgg ttgatattaa ctttaccatc
     5881 tttatgctcc tgaatagctg aagatatatt tccagagaga cggcttgcaa tcacagaggc
     5941 atttgttccg tttacaatat gcgatgacgt actacctggt gcaatattac tagccaaatt
     6001 accaaatgct aataatgcac tataaccagc ttgtttgtaa tattcattac gtttcgagat
     6061 atctttctca ttttttgcag aaactaaatt agcaaccata tttccaaatt ctgctaacgc
     6121 ggatgagcgt gcgctcataa aattaccaaa actattccaa ctcattgaag taagtcctat
     6181 ataaaagcaa attaaatttt aaatataaaa taattaatac gatacaaaca ctgttttcaa
     6241 taagaaaaaa tgttttatac ttatctaaca agtacaaact tttgtttcca tcattaattt
     6301 caaatattac attatctata aaatctcctt gaggttgatt ataaaaataa attttaacag
     6361 gtttttttgc aaattttata aaattggcac atggagcata tgaatgaata ccacaaacca
     6421 agcttaatct tatggattta tttttaacat cagatgcaac tacatcaata taaggtgttt
     6481 ctctaattac cctaccatat ctatgattat cttttaattt agcatatcca tttgagtcat
     6541 aaacataatt ataactatca tgtggtttta tcaccccagt aatcgaggat atattttgtt
     6601 cctttatttg atgctcaaat ttataaaatt ttatatttat catactaata tgaattacca
     6661 tccatataat aatagaaatc ggcaataaaa acaataattt gttttttatg tttttttcta
     6721 aaaataataa atttacagca agaaagacgt ttgcaaaaaa caaaaacgca aaaaaatcaa
     6781 atagaaaaac aaaataacca aacactatat taattactat caaggctata tgtaaaacta
     6841 taaatactag taaataattt ttatttttca tatgtctcat taaataaaat atagctggta
     6901 tgatttagtg aatatcaatt tagtggcata gagtgtatac tcatcgtatt cactctctca
     6961 gtcttcaggc tgcctgaaaa gcctttggcg gtgactgttt ctatgccgtc gccgtccaag
     7021 tctagggcga ggggatcgta gacgtggtat tttccatcac acgcagaaaa ttttttgcat
     7081 tgtttagtat caccactacc aatcattgtt actctccttg agtttaagtc aaacaaaaaa
     7141 tgaaattcaa atactctctc ttaacttatt gagatcaatc atcaatataa ataaaaaata
     7201 taaactacca gaataaccaa agccacgaaa aattttttcc aatatttcac tatttaaaca
     7261 aaaagcaccg catacccacg ttgatgattc cgaaataaaa cacatccata tttccattac
     7321 tgccaaccat gacactacta tcctaatcaa aaatactttt ttatctgccc aagcactttt
     7381 tatatttaac aatacaatat gattttgtaa tttaaaatct aagctttgca tttttctaac
     7441 cagtgcaaac aaacaaccaa gcgcaataaa aaaagattgg taggactggt aaaaaggtag
     7501 aaatagaata tttaagacca atggatagat ccacatatac ctatttgtta aattattaat
     7561 aaattctttt ttgttatcca taaaatattg cgctgatatt gcactgagtt tattagcaaa
     7621 caacgtaaag aaaatattta ttatgataag tgttttttaa gtttcatttt caaatgatgg
     7681 cgcgtaataa aaagtttcac aaatccgttt ttcagactat ctaagtatct attttacaat
     7741 ctatttcaat aatcgttatg tcttaacaac tcttcttggc attatagatg ctcactcata
     7801 tcatttaatg atactcttga ttaatcttta tgcgatttag cgaaattaaa atttctcttt
     7861 tttgtttgag gctgtactag attagcagat atgttacccc cgaaatatga agataacaca
     7921 ctgcaaatta aagaaaaaag tacagaaaga gctgctccgt ttttttgtac tcgaagttac
     7981 cgcccgttct gctgccgata ttttgggtat ccatcccaat tcggcagtcc tgttctaccg
     8041 caaaatccgt atggtcatca gccattatct ggccttggct gccaatgagg ttttcgaggg
     8101 ctctgtcgag ttggacgaaa gctatttcgg cggacggcgt aaaggca
//
LOCUS       DAXHRI010000086         8109 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXHRI010000086 DAXHRI010000000
VERSION     DAXHRI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.086"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="ACFM75_001375"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="ACFM75_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC6255911.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTRELVHQINQYLTEKKRIEAEKAEAEQAAAKAEIAEQQPKTDTGVFKPE"
     gene            1607..1858
                     /locus_tag="ACFM75_001376"
     CDS             1607..1858
                     /locus_tag="ACFM75_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255912.1"
                     /translation="MISDKQRTELAKKQVMLKILYQAWLAEKRKYAVITVVDNEGNLI
                     EYHPSGNQRTVGHVKQLAYFSRSKNVRQPLDRVKGHLKI"
     gene            1932..2684
                     /locus_tag="ACFM75_001377"
     CDS             1932..2684
                     /locus_tag="ACFM75_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFC6255913.1"
                     /translation="MEKDTLAEFRNKFLKGDSLEIMKSIPSGSIDLIVTSPPYNLKNS
                     TGNGMKDGRGGKWKNASLINGYSDHNDCMPHHEYVEWQRNCLSEMYRLLKDDGAIFYN
                     HKWRVQGGLLQDRHDIVSGFPVRQIIIWRRKGGINFNKGYFLPTYEVIYMICKPKFSL
                     CPGANKYGDVWEFTQEMKNPHPAPFPVALIERIISSTNAKVILDPFMGSGTTAIAAKK
                     LYREFIGIEISAEYIALAQERLNETLREPKLL"
     gene            2681..3463
                     /locus_tag="ACFM75_001378"
     CDS             2681..3463
                     /locus_tag="ACFM75_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HFC6255914.1"
                     /translation="MKIYTKETLIAALKEIRDLNWVKSARPGNDGSVGNTLEDLLGIE
                     ENNLPIPNAAEWELKAQRKDSSSLLTLFHMEPSPTSLKLVPNLLLPNYGWPHKNAGEK
                     YPNTEKSFRQTINAKNYSNRGFKVHVNRDEEKVEVSFNQQFITEENILWKQYVNEKIG
                     LNELSPQPYWGFNDLFHKAGTKLHNCFYIQAARKRANGEEYFHYQDIFMLKELSKEKF
                     IQAIEDGHVYVDFDARTGHNHGTKFRIKPSLIPSLYSEVIIL"
     gene            3488..4909
                     /gene="cysS"
                     /locus_tag="ACFM75_001379"
     CDS             3488..4909
                     /gene="cysS"
                     /locus_tag="ACFM75_001379"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC6255915.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGRGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHNHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDLEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTAFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            5080..5859
                     /locus_tag="ACFM75_001380"
     CDS             5080..5859
                     /locus_tag="ACFM75_001380"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6255916.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYCFLDAFYPMLEAMKNEGRDIVVCGDWNVAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5846..6190
                     /locus_tag="ACFM75_001381"
     CDS             5846..6190
                     /locus_tag="ACFM75_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255917.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWQGWRLLANVLACLAVRTLLSVPAA
                     LLIAFTLQDLLK"
     gene            6200..6730
                     /locus_tag="ACFM75_001382"
     CDS             6200..6730
                     /locus_tag="ACFM75_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6255918.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6959..8074)
                     /gene="asd"
                     /locus_tag="ACFM75_001383"
     CDS             complement(6959..8074)
                     /gene="asd"
                     /locus_tag="ACFM75_001383"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC6255919.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMQEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2145 a         1982 c         2101 g         1881 t
ORIGIN      
        1 gtccacagga caatggtggt cggaatgccg atgaaaccgg caacggtcag ccagtgcagc
       61 gtacgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcggacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggt agcgtctggg cggaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgtcaaa cgctaccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 accctcatcc gcgacctcga caccgacgaa atcgttgccg acctcactta ccacggacag
      721 cgcgtctgcc tagccaaagg cggcaaaggc ggcttgggca acatccactt caaatcctcc
      781 gtcaaccgcg ccccaaaaca atccacgccc ggcgaagaag gcgaagcccg ttccctgcaa
      841 ctcgaactca aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccaa acttaggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg cgcggcagaa ggcgcaggcc tcggccatcg tttcctcaaa
     1081 cacttatcac gtaccggcct gctgctgcac gtcgtcgatt tggccccgtt tgacgaaacc
     1141 gtcaaccccg ccgaagaagc cctcgccatc atcaacgaat tgcgcaaata cgacgaagaa
     1201 ctctacggca aaccacgctg gctggtgctg aacaaactcg acatgctcga tgaagaagaa
     1261 gcccgggcgc gaacagccgc cttcctcgaa gccgtcggct gggactaccc gaaaccagac
     1321 gaccgcttcc aattcgacat ggaaaccccg cgcctcttcc aaatcagtgc tctgacccac
     1381 caaggcacgc gggaattggt acaccaaatc aaccaatatc tgactgagaa aaaacgcatt
     1441 gaagctgaaa aagccgaagc ggagcaggca gcggcaaaag ccgaaattgc cgagcagcaa
     1501 cctaaaacgg atactggcgt gtttaaaccg gagtaaaaaa tttcagacgg cctttttaat
     1561 acaatatagg actgtccgaa tacaggaata aaaagcaagg aagagcatga tttctgataa
     1621 acaacgtacc gaattggcga aaaagcaagt catgcttaaa attttgtatc aggcatggct
     1681 ggcagaaaaa cggaaatacg ccgtaatcac ggtagtcgat aacgaaggca atctgatcga
     1741 atatcatcct tccggtaatc aaagaacagt aggccatgtc aaacaactgg cctatttcag
     1801 tcgaagtaaa aacgtcaggc aacctttgga tagggttaaa ggtcatctaa aaatttagtt
     1861 attactgaat atgtcatgat taaattaaat accttgtaaa tattaataat tttgaaatga
     1921 agaatttaaa tttggaaaaa gacactctgg cagaatttcg taacaagttt cttaaaggtg
     1981 attctttaga gattatgaaa tcaataccct cgggatctat cgatctgatt gttacatcac
     2041 cgccttataa tttaaaaaat tctactggaa atggaatgaa agatggtcga ggtggaaagt
     2101 ggaaaaacgc tagcttaatt aatgggtatt ctgaccataa tgattgtatg cctcatcatg
     2161 aatatgtgga gtggcaacga aactgtctgt ctgaaatgta tcgactactt aaagatgatg
     2221 gggctatttt ctataatcat aaatggagag ttcagggggg attattacaa gatagacatg
     2281 atattgtctc tggttttcct gtaagacaaa ttattatttg gcgtagaaaa gggggaatta
     2341 atttcaataa aggatatttc cttcctacat atgaagtaat ctatatgatt tgtaaaccga
     2401 aattttccct ctgcccgggt gctaataaat atggagatgt gtgggaattt acccaagaaa
     2461 tgaaaaatcc tcatcctgcc cctttccctg tagcactgat agagagaata attagcagta
     2521 caaatgctaa agtcatttta gatcctttta tgggtagtgg cacaacagct attgctgcaa
     2581 aaaaattata tcgtgagttt attgggattg aaatttcagc tgaatacatt gcattagctc
     2641 aagaacgact aaatgaaact ttgagggaac caaaattatt atgaaaattt atactaaaga
     2701 gactttgatt gctgctctga aagaaattag agatttaaat tgggtaaaaa gtgctcgccc
     2761 tggtaatgat ggttccgtag gaaatacctt agaagattta ttaggtattg aagaaaataa
     2821 tttacctata cctaatgctg ctgaatggga acttaaggcg caaagaaaag atagttcctc
     2881 attattgaca ttatttcata tggagccttc ccctacctcc ttaaaactgg tacctaattt
     2941 attgttacct aactatggtt ggccacataa aaatgctggg gaaaaatacc caaatacaga
     3001 aaaaagtttc agacaaacga ttaatgcaaa aaattattca aatcgtggtt ttaaagtcca
     3061 tgtaaataga gacgaagaaa aggttgaagt ctctttcaat caacagttca ttacagagga
     3121 aaatatttta tggaaacaat atgtaaatga aaaaatagga ttaaatgagt tatctcctca
     3181 accttactgg ggttttaatg acttatttca taaggctggt actaagttgc ataattgctt
     3241 ttacattcaa gctgcaagaa aaagagcaaa cggggaagag tattttcatt atcaagacat
     3301 ttttatgttg aaagagctgt caaaagaaaa gtttattcaa gctatcgaag atggtcatgt
     3361 ctatgttgat tttgatgctc ggactggcca caaccatgga acaaagttca gaataaaacc
     3421 ttctttaatt ccttctttgt attctgaagt catcatttta taaacttcct aagctaacat
     3481 tattcatatg accaccatct acaacaccct tacccgtcaa aaagaaccct ttgcccccat
     3541 cgaccctgaa aacgtgcgta tgtacgtttg cggcatgact gtttacgact attgccactt
     3601 aggccatgcc cgtgtgatgg ttgtgttcga catgattgcc cgctggctgc gcgagtgcgg
     3661 ttatccgctc acttatgtgc gcaacatcac cgacatcgac gacaaaatca ttgcccgcgc
     3721 ggctgaaaac ggcgagacca tcggcgaact gaccgcgcgt ttcattcagg ctatgcacga
     3781 agatgccgat gctttgggtg tgttgcgtcc cgatatcgag ccgaaggcga cggaaaacat
     3841 cccacaaatg attgccatga ttgaaaccct gattcaaaac ggcaaggcat atcctgccgc
     3901 aaatggcgac gtttactacg ccgtgcgcga gtttgccgct tacggccaat tgtcgggcaa
     3961 atcgttggac gacctgcgcg cgggcgaacg tgtggaagtg gacggtttca aacgcgatcc
     4021 gcttgatttt gtgttgtgga aagcggcgaa agcaggcgag ccggcatggg aaagcccgtg
     4081 gggcagggga cggccgggct ggcacatcga atgttctgcg atgagtgaaa acctgttcgg
     4141 cgatacgttc gacattcacg gcggcggcgc ggatttgcaa ttcccgcatc atgaaaacga
     4201 aatcgcccaa agcgtcggtg ccagcggtca tacctgcggc cacaaccacg cgcaaaccca
     4261 ccacggtcaa agcattgcca gccacgtcaa atactggctg cacaacggct ttatccgcgt
     4321 ggatggcgaa aaaatgtcca aatcgttggg caacttcttc accatacgcg aagtgttgaa
     4381 acaatacgac ctcgaagtcg tgcgtttctt tattttgcgc gcccactacc gcagcccgtt
     4441 gaactattcc gacgcgcatt tggatgatgc aaaaggcgcg ctgacccgtc tgtacaccac
     4501 cttgaaaaac acgccggcgg ctgagtttga tttatccgaa aacgccaacg gctacacccg
     4561 ccgcttctac gccgccatga acgacgattt cggtacggtc gaagcggttg ccgtgttgtt
     4621 tgagctggca ggcgaagtga acaaaaccaa tgacgcacag ctcgccggct gcctgaaagc
     4681 cttgggcggc atcatcggac tgctgcaacg cgatccgacc gcgttcctgc aaggcagcgc
     4741 ggcttcagac ggcctctcca acgaagaaat cgaagacttg atcgcccggc ggaaacaggc
     4801 gcgcgccgat aaaaactggg cggaatccga ccgcatccgc gaccttctga acgaacacaa
     4861 aatcattttg gaagacaacg ccggcggcac gacttggcgg cgcggttgaa ctgtatcgga
     4921 aagggcaaaa gtaccgcctt ttgctctttt tccgttttca gacggcattt cccgcttgaa
     4981 gccccgccgt tttcagcagg cagggccgaa tgggaaatgc cgggcggcgc accgggtgct
     5041 gctttgtgta tgggaacatt gtgagaactt atttttatta tgcttaaaat catttccgcc
     5101 aacgtcaacg gcatccgctc cgcctacaaa aaaggttttt acgaatacat cgccgcatcg
     5161 ggcgcggaca ttgtctgcgt gcaggaactc aaagcgcagg aggctgattt gtctgccgat
     5221 atgaaaaatc cgcacgggat gcacggccat tggcattgcg ccgagaagcg cggttacagc
     5281 ggcgtggcgg tgtacagcaa acgcaaaccc gacaatgtgc aaatcggtat gggcattgaa
     5341 gaattcgaca gggaagggcg gtttgtgcgt tgcgatttcg gcaggttgag cgttatttcg
     5401 ctttatttgc ccagcggcag cagcgcggaa gaacgtcagc aggtgaaata ctgtttcctc
     5461 gatgcgtttt atcctatgct cgaagcgatg aaaaacgaag ggcgcgacat cgtcgtctgc
     5521 ggcgactgga acgtcgcaca ccaaaacatc gacctgaaaa actggaaagg caaccagaaa
     5581 aattcaggtt tcctgcctga agagcgcgag tggataggca aggttatcca caagctcggt
     5641 tggacggata tgtggcgcac gctttatccc gatgtgccgg gctacacttg gtggagcaac
     5701 cgagggcagg cgtatgcgaa agatgtcggg tggcgcatcg attatcagat ggttacgccc
     5761 gaacttgctg ccaaagccgt gtccgcacac gtttataaag atgaaaaatt ttccgaccat
     5821 gcgccgctgg tcgtggagta tgactatgct gccgaataaa gttttgggta aatatgattg
     5881 gaatgtggac ggaaaaacag gaataggcgc ggcatgggtt gtcgctgcgt ttatcctgcc
     5941 catattggtg tgggcgatat ttatgttgtc gcggatgcaa ggctggcttg cgccgaccaa
     6001 ggcaaacccg atatgggcgt tggtgtggct gctgatctgc ctgccctgcc tgctgattgc
     6061 gtccaaatgt ttgggttggc aaggctggcg gctgttggca aacgttcttg cctgcctcgc
     6121 cgtgcgcacc ctgttgagtg tgcctgccgc gctgctgatt gcgttcacgc tgcaagacct
     6181 gctcaaatag gcgcggacaa tggaaagttt cgagcttgag gatttgacgc tgtggctggt
     6241 acgcgatgca gatgaagcgg aaatgtggat agaccgctgg gcgattagtt atcccgtcgt
     6301 gcagatgtcc gaagcgtcgg ccggtcaaag cataggggaa tggcaggcag ggcttcagac
     6361 ggcatttgaa cgcatacgcg gcaaatacgt cgccgttgtc gcacacggtg cgggcgcggc
     6421 cgcatttttg gcgtggctgt atcaggcaga catcctgaca cggaagaaaa ttgccaacat
     6481 catccttgta ccgcagcgtc ccgatatttt tcccgacgat gcggaacaca ctttccaacg
     6541 cgtccgctgt ccctgccgtg ccgcattggt tgtacccgaa cacggcggcg tgccgcacgg
     6601 ttgggcgcaa aaacaggcgg atttgtggaa cgcccgcctg ttggtttccc cgcattcggg
     6661 cagtttgaac ggcatgctcg gcggctggca gtggggtatg aagctgatgc aggaaatgtt
     6721 gctggcgtga acgcttgcct tatcggaatt tcggctgtag caggaagtcc gcatatcggg
     6781 ctgttttcat tgacgtgcag gcgcatcagg ttcggataaa gtcgggctgc cgcatactgc
     6841 cggaaatgga atcctttatt ttttacgtcg gcagtgccgg cagggcgtta tccggcaggg
     6901 ttgcaaggaa aatgccgtct gaaacaggct tcagacggca tttccattca aacagggctc
     6961 acaggctgcc caacacgata cgcaatacgc ggcgcagcgg ttcggccgcg ccccacaaga
     7021 gctggtcgcc gacggtgaac gcgctgatgt attcgccgcc catgcccagt ttgcggatgc
     7081 gtccgacagg gacggacagc gtgccggtaa ctttggcagg agtcagctcg tggatgctgg
     7141 cttctttttc attggggatg actttcaccc agtcattcgc gcctgccaaa atcgtttcga
     7201 tttcggaaac aggcaggtct tttttcaact tcagagtgat ggcttggctg tggcagcgca
     7261 tcgcgccgac gcggacgcac aggccgtcaa tcacggttgg attgtcgctg cggccgagga
     7321 ttttgttggt ttccacgccg cctttccatt cttctttgga ctggccgttg cccaaatcca
     7381 cgtcaatcca cggaatcagg ctgccggcga gcggtacgcc gaagttggct ttcggatagt
     7441 cttcgctgcg caggaaatcg gatactttgc ggtcgatgtc gagaatcgag ccggcaggat
     7501 cggcaagttc gtccgccact tgggcgtgaa ccgcgcccat accgctgatg agttcgcgca
     7561 tgtttttcgc acctgcgccg gaagcggctt ggtaggtcat gctggttgcc cattcgacca
     7621 aatcgttttg gaacaggccg cccaaagcca tcagcatcag ggagacggtg cagttgccgc
     7681 cgatgtagtt tttcacgccg tttttgaggc cgttgtcgat aacgttgcgg ttgacagggt
     7741 cgaggacgat aatcgcgtcg tctttcatgc gcagagaaga tgccgcgtca acccagtagc
     7801 cgttccagcc gctgtcgcgc agggcttgga acacggattt ggtgtaatcg ccgccctgac
     7861 aggtaacgat gatgtccatt tttgccaatt cggcaacatc gttggcatcc aacaatgttt
     7921 tggctgcctg accgaaatca ggggctgcgc cgccgacgtt ggaagtggta aagaaaaacg
     7981 cttcgggaat gtgggcgaag tcgttttctt cttgcatacg ctgcatcaaa accgaaccga
     8041 ccatgccgcg ccagccgacg aaacctactt tcattgtgtt gctccttgag gaaaaaatcg
     8101 ttaatatag
//
LOCUS       DAXHRI010000087         7996 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXHRI010000087 DAXHRI010000000
VERSION     DAXHRI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.087"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(142..2184)
                     /gene="recG"
                     /locus_tag="ACFM75_001384"
     CDS             complement(142..2184)
                     /gene="recG"
                     /locus_tag="ACFM75_001384"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC6255920.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLNTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2271..2714)
                     /gene="secB"
                     /locus_tag="ACFM75_001385"
     CDS             complement(2271..2714)
                     /gene="secB"
                     /locus_tag="ACFM75_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC6255921.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSSTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2735..2992)
                     /gene="grxC"
                     /locus_tag="ACFM75_001386"
     CDS             complement(2735..2992)
                     /gene="grxC"
                     /locus_tag="ACFM75_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC6255922.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3205..4698
                     /gene="rng"
                     /locus_tag="ACFM75_001387"
     CDS             3205..4698
                     /gene="rng"
                     /locus_tag="ACFM75_001387"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC6255923.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAASNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4662..5984)
                     /locus_tag="ACFM75_001388"
     CDS             complement(4662..5984)
                     /locus_tag="ACFM75_001388"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6255924.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAAWQSVLPLLAALPLMILLTVWITHQAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVRGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGAVDCRHACQKIRRTF
                     GIDRQPTFRTRSVDTGVVGQGNPEIDRCRLKASDGIFYISTISYCSCV"
     gene            complement(5980..6155)
                     /locus_tag="ACFM75_001389"
                     /pseudo
     CDS             complement(5980..6155)
                     /locus_tag="ACFM75_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6378..7691)
                     /locus_tag="ACFM75_001390"
     CDS             complement(6378..7691)
                     /locus_tag="ACFM75_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC6255925.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1885 a         2358 c         2079 g         1674 t
ORIGIN      
        1 ccgataagtc tctgcggctt cccattgccg gatttccgcc cacttgggaa taacgagatt
       61 taaaatcatg aaaatgtgtc aaaaataatg caaaatcaga ttccgttgca cttttttcca
      121 atatttcaga cggcattttg ctcacacgcc caaataacct tccctgccgg aaagccatct
      181 tgccaaatgc gcttcgacga tttcggggtt ttgctcaatc agtatcgggg cgatttcgcg
      241 cgcttgttcc aagaggtgca ggtcttcttc gagcttggcg aaacgcagca taggcacgcc
      301 gctttggcgc gcgccgagaa attcgccggg gccgcggatg ttgaggtctt ggcgggcgat
      361 ttcaaagccg tcggtgtgtt cgtagatgac tttcagccgc gctttggcga gttcgcccaa
      421 gggttcggca aacaggagga cgcacacgct ttctgccgcg ccgcgcccga cccgaccgcg
      481 taattggtgc agctgcgcca agcccatgcg ctcggcgtgt tcgatgacca tcagggcggc
      541 attgggcaca tctacgccga cttcgatgac ggtggtggcg accaagacat tcagcccccc
      601 cgaagaaaac cgcgccatca cttcggcctt ttcggcggcc ttcatgcgcc cgtgtaccag
      661 tccgatattg agttcgggca atgccgtctg aagccgggcg agggtttcgg cggcggtttg
      721 cagttgcagg gtttcgcttt cttcaatcag cggacaaacc caatacgcct gccgcccttt
      781 gcggcaagta ttgaggacga agccttcgac ttcggcgcgg cggacgttgt tgacgaggcg
      841 cgttttaatc ggcgtgcgcc cgggcggcag ttcgtcgatg acggacacgt ccaaatcggc
      901 gaaaaaactc atcgcaagcg tgcgcgggat gggcgtggcg gacatcatca gctgatggac
      961 ttcgcgccct ttgtttttga gggcgaggcg ttgggcaacg ccgaaacggt gctgttcgtc
     1021 cacaatggtc aagcccaaat tgtgaaacgc cacgccgtct gaaaacaggg cgtgcgtgcc
     1081 gacggcgatt ttgacgctgc cgtcggcgag tttggctttg gcttcgtctt tggctttttt
     1141 acgcaaactg ccaaaaaggc ggacaacttc aatgcccaaa ggttcgagcc attgtttaaa
     1201 tttaataaaa tgttgttcgg caaggatttc agtgggcgcc attacagcca cctgcgcacc
     1261 ggattcgatg gcggtcagcg cggacaaggc ggcgacaatg gttttgccgc tgccgacatc
     1321 gccctgcagc aggcggtgca tcgggtaggt ttgcgccata tcgcggcaga tttcggaaac
     1381 aactttttct tgcgcatcgg tcagggcaaa cggcagggct tggcgcaggg cttgggtcaa
     1441 tgtgccgtcg ccgcccaatg ccgccgccgt gccgccgata cgcttctgtc gcgccaagcg
     1501 catcgaaagc tgttgcgcca aaagttcatc gaatttgagc cgttgccatg caggcagcgt
     1561 gccgtctgaa agctgatgaa tcgtgaaact cggcggcggc gaatgcaaaa gacgcaggct
     1621 ttcggcgagg tgtggcagct tcagacggca cagcagggca tcgggcagcg tgtcgtgcag
     1681 cggcgtaacg tccaacgccg tctgaataat acggcgcaaa gtgggctggt tcaaaccgtt
     1741 tacggtcggg taaaccggcg tgaggctttc cgccaaaccg ccgccctcgg catcgcggat
     1801 tttgggatga atcatctcgt cgccgtaaaa gccgtgtttg atttcgccca cggcgcggat
     1861 gcgcttaccg accgccgtct gtttctgatg gctggcgtaa aagtggatga agcgcagaaa
     1921 aaggacgctg ccggaaccgt cggcgatttg gacaatcaac tgcttgcgcg gtttgaacgt
     1981 tacttcctga tggataacct cgccctcgac ctggcacggc acgccaatcg gcgcgtcctt
     2041 aatcggcata atgtgcgtct cgtcctcgta acgcagcggc aggtgcaaca ccaaatccca
     2101 cgcggtatgg aggttgagtt tgtcgagctt cttggcggaa acatcggtga ttttgagctg
     2161 tttctgggtt tcgggcgaca tcataggcag attcctttga acgcacctat tttatccgaa
     2221 aacaaaaatg ccgtctgaaa cggattcaga cggcatcgga caggcaggaa tcaagccccg
     2281 gcggcttcgg cttcctgctg ttgttggtaa atcgcctcaa aattaatcgg cgcgagcagg
     2341 acgggcggga agccggcgcg cgttaccgta ctggaaacgg cttcgcgcgc gtaagggaag
     2401 aggatgttcg gacacgccac gcccaacagc agctgcacat cttcttcggg gatgttttcc
     2461 aaacggaaaa tgccgctttg ggttacttcg ttcaaaaaca tcgtgcgctc gttatccaat
     2521 ttggcggtaa cggttacggt aacgtccacg ttgtagtagc cgtcttccag cttttggctg
     2581 ccggtggaaa cgcgcatatc cacttcaggc tcgccctgtt ccaaaaagat ttgcggcgcg
     2641 tgcggcactt ccaaagacaa gtctttgaca tacagtcgct cgatgctgaa tacgggttgc
     2701 agttcttcgc tcattttgtt ttcctagttg ggggttaagg gttcagcagt ccgtccagcc
     2761 cgccttcctg ctggaggcgg tagaggtcgg taaatccgcc gacgtgcgtt tcgccgatga
     2821 aaatctgcgg cacgctgcgc tgtcccgaaa gctgctgcat ttcggcaaag gcttcggggc
     2881 ttgcatcgac acggatttcg tcgatatgtc cgacacctgc cgcgtgcagc agccttttcg
     2941 ccatcgcgca gtaggggcaa aacggacctg tgtacatggt aacggtctgc atattgggtt
     3001 tccgaaagtt ttgcaatgat aatcaatata ggggcatttc ccctgtttgg caagtgcgga
     3061 acagatgcac gttcaaacgg catgtgcgga atgtgtcaaa gtttcttttt taaagtatga
     3121 tagacattgt gaaaaatatt tttgcacccg cgctgcgcgg cggaacggat gcaaaatatt
     3181 tttattacat tttcaggaaa aaccatgttg tcaggactcc ccatccccaa agacatcgcg
     3241 cgcccgcccg aaacgatatt ggtcaacatc acgccgcagg aaacgcgcgt agcggtgttg
     3301 gaggaaaaca atatctgcga gctgcacatc gagcgcaaca gcgaacacag cctggtcggc
     3361 aatatctatc tgggcgtggt gcgccgcgtg ctgcccggga tgcagagcgc gtttatcgac
     3421 atcggcttgg aacgcgcggc gtttttacac atcgtcgatg tcctcgaaca acgccgcaac
     3481 cccgaagaaa cccagcgcat cgaacatatg ctgtttgaag ggcagtctgt tttggtgcag
     3541 gtcatcaaag acccgatcaa caccaaaggc gcgcggcttt ccacccaaat ctcgctggcg
     3601 gggcgtttcc tcgtccatct tccgcaagaa gaccacatcg gcgtgtccca acgcatcgaa
     3661 gacgatgccg aacgcagcag cctgcgcgaa cgcctcgaca agctcctgcc ggaaaatgcc
     3721 tgccgaggct acatcatccg caccaacgcc gaaaacgcca ccgacgaaca gctccagtcc
     3781 gacatcgact acctgaccaa agtgtgggaa cacatccaag aacaggcgaa aatccggccg
     3841 ccggaaaccc tgctttatca ggatttgccc ttgagtttgc gcgtattgcg cgatatggtc
     3901 ggctgcgaca cgcaaaaaat cctcgtcgat tccaccgtaa accacggacg gatgacgcgt
     3961 tttgccgaac aatacgtcca cggcgcattg ggcaggatag aattgttcaa aggcgaacgc
     4021 ccgctgtttg aaacccacaa cgtcgagcag gaaatcagcc gcgccctgca accgcgcgtc
     4081 aacctcaact tcggcagcta cctgattatc gagtccaccg aagcgatgac cacgatagac
     4141 gtgaacaccg gcggcttcgt cggcgcacgc aacttcgacg aaaccatctt ccgcaccaac
     4201 ctcgaagcct gccacaccat cgcccgcgaa ttgaggctac gcaacctcgg cggcatcatc
     4261 atcatcgact tcatcgatat ggcgcaggaa agccaccgcg aagccgtgtt gcaggagctt
     4321 gccaaagccc tcgccttcga ccgtacccgc gttaccctgc acggttttac cagcctgggg
     4381 ctggtcgagc tgacgcgcaa acgctcgcgc gaaaacttaa accaagtcct ctgcgaaccc
     4441 tgcccttcct gccaaggcag aggccgtctg aaaacgccgc aaaccgtgtg ctacgaaatc
     4501 cagcgcgaaa tcgtccgcga agcgcgccgt tacgatgccg aaagtttccg catcctcgcc
     4561 gcctccaacg tcatcgattt gtttttggac gaagaatcgc aatccttggc aatgctgata
     4621 gatttcatcg gcaaaccgat ttctttggca gtcgaaaccg cttacacgca ggaacaatac
     4681 gatatagtgc tgatgtaaaa aatgccgtct gaagccttca gacggcatct gtctatttca
     4741 gggtttcctt gtccaacaac gcccgtatca acagaccgtg tccgaaacgt cggctgtcgg
     4801 tcaattccaa atgtccgccg tattttttgg caagcgtgtc ggcaatcgac agcccccagc
     4861 cccgtcccct gctgctccgt tcccaaaata cggtaaaacg gatcgaggac gcgggcgcgt
     4921 tcggattcgg gaatgccgtt cccgttatct tccacccaca cggcaagata tttcccttcg
     4981 tccgtgaaac ccaaatctat cctgccttca ggcggcgtgt aacgtaccgc gttatcggca
     5041 aaggttttaa tcagcgtata gatttctgtt tcgtcggcag acacttcgac atcgcctctg
     5101 accgccacac cgatgtcctg acgtttttcc aaagccagcg gcatcagttc ctgcaacact
     5161 tggcggaaac ggctttgcag accgaatgtc gttttcgtca gaggggtttc gtccgactgc
     5221 gaacgcgcca gtgccaaaag ctgttcgagc aggtgtttgt tgcgcctgat gctctgctgc
     5281 aaaacggcag gctgccgcgc tgcatcgggt gggagcgaca tattgttgag ccgttccgcc
     5341 tgaagggaaa gggctgtcat cggcgtacgc aattcgtgtg ccgcgtcggc gacaaaccgc
     5401 tgacggtggc ggatgtcttc atccgcacgt ttcaaaagca ggttgatggc ggttacgaaa
     5461 cctctgattt cactgggaat attgtccaca ctcaaagcag acaggtcatt gattcggcgt
     5521 tgttcgagac tttgcgacaa tttgcggacg gggcgcatcg cctgatgcgt gatccacacg
     5581 gtcagcagta tcatcagcgg caatgccgcc agcagtggca acacgctctg ccacgccgca
     5641 tccgccgcca aatcttcacg gtattcgttt tcctgcataa cggcaatccg tccctgttcg
     5701 gtcgtgcgga tatagacgcg gtaataatcg tcgtccccgt ccgcctgaag cgtgtgcaga
     5761 ccgtccgcca gatgcgcagg caggctgaca acagggtctt cctgctgcgg catctgtacc
     5821 aaaatacgcg tatcgccgtc gccctcgggc aaagtttcgg gtttggaatc gggggcgacg
     5881 tacaatgccg cctgacggag caggtcgtcc tgcaacgctt ccgtttcgtg gaaggtttcg
     5941 tagtaggaaa acatacctgc aagcattgcc agcggaacaa acatccaaac cgtcccgccc
     6001 cggcaaaccc aaatccagca gcatcaagtc ataaggctgg gcagcggcag ccgcgcgccg
     6061 tttttgaccc aatccaccgc atagccgccg tctttcaaac ttgccgacac cgcctccgca
     6121 atcatcgcat cgtcttccac cagcaaaaca cgcatcaact ttcccttcaa aataaaccgt
     6181 gcctattcta acaccccaaa attagccgca atttagcggt ctttacgctt gccggtattt
     6241 ttcaaaactg cagcacaaaa aaaccgcgcc ggcaactgcc ttcagacggc attggggcgc
     6301 gattgcaaca cacgggcagg caggcagagc ctgcgacaga ccacaggaac gattcgggct
     6361 tcagacggct tcgccgttta cggcagaggc acgattcctg ccgctatcga actggccaat
     6421 atcgccagcg acaaacccca cgcccagaaa aacgaataac ggatgtgttt gcccatcgac
     6481 aatttcgcca aacccaagcc catccacaaa gccggcgaaa gcggcgtaac aaaagtgccg
     6541 acgatactgc cgatcaacat cgcataacct gctgcttcag gcgccacgcc cgcctgcgag
     6601 gtaatctgct ccacaatcgg aaacagtccg aaataataag cgtccgtact caaaaccaac
     6661 tcaagcggaa tgcccaacac accgatggca atatgcagat aaggcagcag cgcgtccggc
     6721 aggatatgca caatgtcttt ggaaatcgcg tccaacatcc ccgcaccctt caaaatcccc
     6781 aaaaacgtac ctgccgccaa aataatggac gccatcatca ccgcgccgcc ggcgtgggca
     6841 taaatccgct ccatctgttc ctgcgggctg cggtaattca aaagcaacgc cgccgttgca
     6901 gccagcataa atacataacc cggcgggaag atgcccgaaa aaagcaggct catcgccgcc
     6961 aaaaacagca ggacattcca ccaaaacagt ttcggacgcg ccaatttttg ttcttcttcc
     7021 gacaaaggca ccggctttat caaatccgcc acggcgggca acgcgcccaa ctcccggaca
     7081 atccgccttt tttcacgcac acccaaaagc agggacagcg caaggataaa cactacaccg
     7141 ataatttgca ccgtcaacaa aggtttatac aattcgccca catctgcgcc caacacgctt
     7201 gcaacccgcc cagtcggccc gccccacggc agaaggttaa tcaatcccgc actggaagtc
     7261 agcagcaaaa acagcaggta aggattcata tgcagacgct tgtaaagcgg caaaagggcg
     7321 gggacgacca ataaaaacgt cgtcgcaccc gccccgtcca actgcgccac caccgacacc
     7381 aagaccgtcc ccacactcac tgccacgata ttaccccgag tcagcttaat caaaccgcct
     7441 atcatcggac ggaacagccc cacatcgttc atgattccaa aaaacaaaat ggaaaacata
     7501 aacataatca caatctgcat caccgatttg gtgccgcccg aataaaattc ttttaattgg
     7561 gatacatcaa accccgccag caacgcccca aacagcggca ccaagattaa tgcgatgatg
     7621 ggcgacactt tttccgtcag cagcaaccat acgatgaccc cgataatcag cagtccgata
     7681 aacgtcagca tcatttctcc tttattttat tttaaacaga aaaccgaccg tgcaggcaaa
     7741 accgcccaca gacgcgggat aagccttgca ttctactttt ttattttgaa acaagtcaat
     7801 cggtcatttc ctcccattta cgcctgccgc cattcctgta tccgtccgtc atttcacggc
     7861 ggcaaccgat acggaacaac cggcaaaccg gtatcgggac ggcgcggggg cattcatccc
     7921 ggtgcgccga ttcaaacgaa accgccccta tcattgcgga gcgcggggcg tgccgtacac
     7981 gcgggatttt atagtg
//
LOCUS       DAXHRI010000088         7892 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXHRI010000088 DAXHRI010000000
VERSION     DAXHRI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.088"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..1711
                     /gene="mafB"
                     /locus_tag="ACFM75_001391"
     CDS             <1..1711
                     /gene="mafB"
                     /locus_tag="ACFM75_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6255926.1"
                     /translation="AALIQPALAADLAQDPFITDNTQRQHYEPGGKYHLFGDPRGSVS
                     DRTGQINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHGYEEHAPFDNHAADSASKE
                     QGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGARDEYTYHVNGTARSIKLN
                     PTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFINGVAAGAL
                     NPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAAIGGLGSVAGFEKNTRE
                     AVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDFSKSYTCSFHGST
                     LVKTADGYKAIAHIRVGESVFAKDETSGKTGYKPVTARYGNPYRETVYIKVSDGIGNN
                     QTLISNRIHPFYSDGKWIKAEDLKAGSRLFAENGAEQTVQNITLKQQPLKAYNLTVAD
                     WHTYFVKGNQAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRTYSGKGTRQRSQISG
                     AREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNNYNRIESPGKKYRKQ
                     DGD"
     gene            1714..2037
                     /locus_tag="ACFM75_001392"
     CDS             1714..2037
                     /locus_tag="ACFM75_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC6255927.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIEGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            2096..2323
                     /locus_tag="ACFM75_001393"
     CDS             2096..2323
                     /locus_tag="ACFM75_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255928.1"
                     /translation="MDIKFNTLGVILNGVNPEEKLIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            2450..2992
                     /locus_tag="ACFM75_001394"
     CDS             2450..2992
                     /locus_tag="ACFM75_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6255929.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVVYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQICYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVGNLSDMNRKPFTW"
     gene            3157..3795
                     /locus_tag="ACFM75_001395"
     CDS             3157..3795
                     /locus_tag="ACFM75_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6255930.1"
                     /translation="MAHIRVGESVLSKDEASGKTGYKPVTAQYGNPYQETVYIKVSDG
                     IGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITFKQQPLKAYNL
                     TVADWHTYFVKGNQAETEGVWVHNSCPPKRAPEYHAGTVSESNFLNAAEKWLGENYKS
                     YPNGRYVSQDGMRQVRYGYHETNSSTHHGHFESYDKPNGRVIKNSAVTIIRD"
     gene            3799..4110
                     /locus_tag="ACFM75_001396"
     CDS             3799..4110
                     /locus_tag="ACFM75_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255931.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DQGFYLFYLNEAGQEQTDTYHETLDSAFEQAEFEFGISKEEWMQSPYVASVENNNQKV
                     I"
     gene            4362..4991
                     /locus_tag="ACFM75_001397"
     CDS             4362..4991
                     /locus_tag="ACFM75_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6255932.1"
                     /translation="MVRTADGYKAIAHIQAGDRVLSKDEASGKTEYKPVTARYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSDGKWVKAEDLKAGSRLLSENGRTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGSQAETEGVWVHNACPPKRTGSSKNEKHGDGGRSQIS
                     AESRIAELKNKIIPGMSKNERLKIERTIRNITKNANRKAKGEEHGRRGR"
     gene            4972..5427
                     /locus_tag="ACFM75_001398"
     CDS             4972..5427
                     /locus_tag="ACFM75_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255933.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITEYKNILETFMYEPPDEF
                     RTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            5434..5490
                     /locus_tag="ACFM75_001399"
                     /pseudo
     CDS             5434..5490
                     /locus_tag="ACFM75_001399"
                     /inference="COORDINATES: protein motif:HMM:NF026864.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin C-terminal domain-containing protein"
     gene            5505..5882
                     /locus_tag="ACFM75_001400"
     CDS             5505..5882
                     /locus_tag="ACFM75_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC6255934.1"
                     /translation="MCELKNFRRNITCFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            5946..7079
                     /locus_tag="ACFM75_001401"
     CDS             5946..7079
                     /locus_tag="ACFM75_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255935.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(7166..7441)
                     /locus_tag="ACFM75_001402"
     CDS             complement(7166..7441)
                     /locus_tag="ACFM75_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255936.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
     gene            complement(7458..>7892)
                     /locus_tag="ACFM75_001403"
     CDS             complement(7458..>7892)
                     /locus_tag="ACFM75_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6255937.1"
                     /translation="HFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSA
                     YPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDNYSTSVGSCIN
                     GFTVQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
BASE COUNT         2453 a         1665 c         1749 g         2025 t
ORIGIN      
        1 ggccgcactc atacagcccg ccctcgcagc ggacttggcg caagacccgt tcattaccga
       61 taacacccaa cggcagcact acgaacccgg cggcaaatac cacctcttcg gcgacccgcg
      121 cggcagcgtt tccgaccgca ccggtcaaat caacgtcatc caagactata cccaccagat
      181 gggcaacctg ctcatccaac aggcggcaat ccaaggcaat cttggttaca ccgtccgctt
      241 ttcaggacac ggatacgaag aacacgcccc cttcgacaac cacgccgccg acagcgcaag
      301 caaagaacaa ggcaacgttg acgacggctt taccgtatac cggctcaact gggaaggaca
      361 cgaacatcat cccgccgatg cctacgacgg cccgaagggc ggcaattacc ccaaacctac
      421 gggcgcacga gacgaataca cctatcacgt caacggcaca gcccgcagta tcaaactcaa
      481 tccgaccgac acccgcagca tccggcaacg catatccgac aactacaaca acctcggcag
      541 caatttctcc gaccgcgccg atgaagccaa cagaaaaatg ttcgagcaca atgccaagct
      601 cgaccgctgg ggcaacagca tggagtttat caacggcgtc gccgccggcg cgctcaaccc
      661 ctttatcagc gcgggcgaag ccttgggcat aggcgacata ctgtacggaa cgcgctatgc
      721 catagacaaa gccgcaatgc gcaacatcgc ccccttaccc gccgagggca aattcgccgc
      781 catcggcggc ttgggcagcg tggcgggctt tgaaaagaat acgcgcgaag ccgttgaccg
      841 gtggatacag gaaaacccca atgccgccga aaccgtcgaa gccctggtca acgtcctgcc
      901 gtttgccaaa gtcaaaaacc tgacaaaggc ggcaaaaccg gggaaggctg cggttagtgg
      961 ggatttttct aaatcctaca cctgctcctt ccacggcagc accttggtca aaacggcaga
     1021 cggctacaaa gccattgccc atatccgagt cggcgagagc gtcttcgcca aagatgaaac
     1081 cagtggaaaa actggataca aacctgttac cgcccgatac ggcaatccgt atcgggaaac
     1141 cgtttacatt aaagtttcag acggcatcgg taacaaccaa accctgattt ccaaccgcat
     1201 ccacccgttt tattcggacg gcaaatggat taaagcggaa gatttgaaag cgggaagcag
     1261 gctgtttgcc gaaaacggtg cggagcagac ggttcaaaac attaccctca aacagcagcc
     1321 gctcaaagcc tacaatctga ccgttgccga ctggcatacc tatttcgtca agggtaatca
     1381 ggcggaaacg gaaggggttt gggttcataa tgattgtccg tatggagaaa aatataggac
     1441 agaagttggt tcttatacta atactcatga aagtggtcga acttattctg gaaagggcac
     1501 aagacaacgt tcccaaatct ctggagcaag agaagctagg gttaataatg accctcatat
     1561 agctacagat tttactcctg ccaaaaataa tcgagaggca ttcaaagatg aatctagaag
     1621 aattgacgct cacggtggtt ccaaaagtac caataattac aatcgaattg aaagcccggg
     1681 gaaaaaatac cgaaaacaag atggagatta aaaatgattg ttgacgaaaa atctacttca
     1741 gatgtactga aacaatatca agaactaggg tttgatttga caaaacctat gataatagag
     1801 ttttttatag agggttctca aaaaaatctg caatcaatag aaagccaact catttcttat
     1861 cggcaagatt ttcaaatcag tattgaacaa gatgaattcg gagaaatgtg gacttgctat
     1921 tgttccgtca atatcgtccc ttcccttgaa aacattttag ctattgaaac tacgttattt
     1981 ggtattgcac aaaaaaatga ttgtaattat gaaggttttg gttcgtatgg gaactaacaa
     2041 aaagtgtagg ttgggttgaa aaaaccatat tcccattaaa gattagggtg tgtctatgga
     2101 cataaaattc aatactttgg gggttatcct gaatggggtc aatcctgaag aaaaactcat
     2161 aaaaattatt gatgatcagg aaaataccgg tggatttctg atactgttat cttcaaatga
     2221 caaattctca tcatttgaca gttacgatga ctgggtcgaa aacttagaaa tattaaaaga
     2281 atacctgcaa gaatctcatt ggataataaa gtgggtcgga tagtcggcaa acgtagtccg
     2341 tctgaaacac ttttactttt cagacggact ttctatcaat gggaacaagg cgttttgtct
     2401 caatctggtc tatgattatt cgatacagtg atgggaagct aagaaaaata tgattaatca
     2461 tattgaaaaa tatataggca ctatttctca tggctcgaaa agcgattcgg gaagtcagta
     2521 caaattaaat atcgcagcta ttccctcttc ccctaacagg gatttaaaaa cttatattac
     2581 tttagggtta agcaaacatg atctgaatta taaaagcaga tttgaaatac ttttcgtatg
     2641 ttctttaaaa tatgatgaaa atcagatttt cccattttta agatggttgg cagaaaccat
     2701 cattgagaac aaaaaaatcc ttcttcgggg gcaggttgtt tatttgccca gaagcattgt
     2761 tgattcaaca aaaatggatg cactgtatgt ttccgcccca ttttatttcg acgatgattt
     2821 tcagatctgt tatggcgaac actacaatat cgttttccct ttgcttgtcc ccttgtataa
     2881 acaggaagcc gaattggtgg aaaagaaagg ttggaatgct tttgagcagt tcttgttgga
     2941 taatgaagtt ggcaaccttt cggatatgaa tcggaaaccg tttacttggt aaggattttc
     3001 ttaaatttga ttaaaggtcg tctgaaaccg tgtttaactt tcagacaacc tttccttcat
     3061 ttgaaacagg atattgggaa ctaagttctt caaaaatcct gtctccgtcc cttatacgtc
     3121 ggtgctttag tcaaaacggc agacggctac aaagctattg cccatatccg agtcggcgag
     3181 agcgtccttt ccaaggacga ggcaagcggg aaaacaggat acaaacccgt taccgcccaa
     3241 tacggtaatc cgtatcaaga aaccgtttac attaaagttt cagacggcat cggcaacagc
     3301 caaaccctga tttccaaccg catccatccg ttttacagtc aaggaaaatg gatacaggca
     3361 ggtcgtctga aaaaaggcga caccctgctt tccgaaagcg gcgcaaaaca gacggttcaa
     3421 aacattacct tcaaacagca gccgctcaaa gcctacaatc tgaccgttgc cgactggcat
     3481 acctatttcg tcaagggtaa tcaggcggaa acggaagggg tttgggttca taattcttgt
     3541 ccgcccaaaa gagctcccga ataccatgca ggaactgtat ctgaatctaa ttttttaaat
     3601 gcagctgaga aatggctagg tgaaaactat aagtcatatc caaatggtcg atatgtatca
     3661 caagatggaa tgcggcaggt acgatatggt tatcatgaaa caaattcatc tacgcatcat
     3721 ggtcattttg aatcatatga caaacctaat ggacgtgtaa taaaaaatag tgcggtaaca
     3781 attattcggg attgaagaat gatgagtgta aaagaattat tcaaagtcat cttagatgaa
     3841 aataaagatt ttcctattag aacgattcat cgtaccccga tggggatact tcccaaacct
     3901 gttgctttaa gtattgttca atatgaaaat gaccaaggat tttatttgtt ttatttgaat
     3961 gaggctggtc aggaacaaac ggatacttat cacgaaacat tagattcggc atttgaacag
     4021 gcggagtttg aatttggaat cagcaaagaa gaatggatgc aaagtcctta tgtagcaagc
     4081 gtagaaaaca acaatcaaaa ggtcatctga aaccgtgttt ggtttttcag acaacctttc
     4141 tatcagtaaa aacgagatgt tgggttttca tccgacctag gcttgcgcag cttaagccaa
     4201 tgataatagg ttaagtaacc gtaggttggg ttaaaaaccc aaccatcaaa atgccgtctg
     4261 aaaccgtttt taatttttag gcgacctttc cttcatttga aacaggatat tgaaaactaa
     4321 gtttttcaac attctacacc tgctccttcc acggcagcac cttggtcaga acggcagacg
     4381 gctacaaagc cattgcccat attcaagccg gagaccgcgt cctttccaag gacgaggcaa
     4441 gcgggaaaac agaatacaaa cccgttaccg cccgatacgg taatccgtac caggaaaccg
     4501 tttacattga agtttcagac ggcatcggca acagccaaac cctgatttcc aaccgcatcc
     4561 acccgtttta ttcggacggc aaatgggtta aggcggaaga cttaaaggcg ggaagcagac
     4621 tgttatccga aaacggcaga acccaaaccg tccgcaacat cgttgtcaaa ccaaaaccgc
     4681 tcaaagccta caatctgacc gttgctgact ggcataccta cttcgtcaag ggcagtcagg
     4741 cggaaacgga aggggtttgg gttcataatg cgtgtccgcc taaaagaaca ggaagctcca
     4801 agaatgaaaa acatggagat ggcggtcgaa gtcaaatatc agcagaatca cgaattgctg
     4861 aattaaaaaa taaaattatt cccggaatgt ccaaaaatga acgattaaag attgagcgaa
     4921 caatcagaaa tattacaaaa aatgccaatc gaaaagcaaa aggagaagag catggtcgac
     4981 gcggtcgtta aaactcccga atttttacct tttacttctg taggaatatt ccgttttggt
     5041 gcggatataa cagagtacaa aaacatactg gaaactttta tgtatgaacc tcctgatgag
     5101 tttagaacag aatattatga atctcccgac tcaaatttac tcatttccgt caaaaaaaac
     5161 aaaattattt cgatattttg ttatcaggag ctatatttta tgggcgttaa tataataggt
     5221 ctgaattttg aagattttaa acagcttttc catcacccta aatcatacgg tgttgataaa
     5281 tattatttat caaatgaatc ttatccgaca tatgtctatg agtttgatga aataggagta
     5341 caagcatggg aagcaaaagg gaaaattgtt actatcattg ccgggggcaa agacaattat
     5401 tcaacagaac cttactacga tgaataacat tacagaaaag aaacaagaaa cggtacattt
     5461 gataaaaacc taaatcgtat tggagattaa ttattatgtg tattatgtgt gagttaaaaa
     5521 acttcagaag aaatattact tgttttgagg gatatgacga aaattcattt attggcaaat
     5581 ggtatgatga cggggtgtgg gatgatgaag aatattggaa attggagaat gatttaatcg
     5641 aggttagaaa aaaatatcct tatccgatgg atataccaag ggatattgtg attggaatcg
     5701 gtaccattat tgatttttta atggttccaa attgggagct tttcgaaatt aaagcttccc
     5761 cttggctacc tgatagcgtg ggaattcatg aacgttatga aagattcaca acaatgctcc
     5821 gttatatttt taccgagaaa gacatagtca acgtgcgatt tgattattac aacaaaaaat
     5881 agcaaaaccc ggcaatcaaa gctcgtctga tataatcgat atattctgtt aaacgaaaaa
     5941 taaatatgtt taaaaaattc aaaccggtac tgttgtcatt ttttgcactt gtatttgcct
     6001 tttggctggg gacgggtatt gcctatgaga ttaatccgcg ttggtttttg agcgatacgg
     6061 caactgaagt acctgaaaat ccgaatgctt ttgtggcgaa acttgcccgc ctgttccgaa
     6121 atgccgacag ggcggttgtc atcgtgaagg aatcgatgag gacggaggaa agtcttgccg
     6181 aaactgtgga tgacggtccg ttgcagtcgg agaaggatta tctcgcgctc gctatccggc
     6241 tcagtcgttt gaaagaaaag gcgaaatggt ttcacgtaac ggagcaggaa catgggaaag
     6301 aggtttggct ggattaccat atcggcgagg gcggtttggt tgcggtttcg ctttcgcaac
     6361 gctcgccgga agcgtttgtt aatgccgaat atctgtatcg gaacgatcgt ccgttttctg
     6421 taaatgtgta cggcggaacg gctcacggag aaaattatga aacgacagga gaatatcggg
     6481 ttgtttggca accggacggt tcggtatttg atgcgtcggg gcgtgggaaa atcggggaag
     6541 atgtttatga gcattgtctc gggtgttatc agatggccca ggtatatttg gcgaaatacc
     6601 gggatgtcgc gaatgacgag cagaaggttt gggacttccg cgaagagagc aaccggattg
     6661 cgtcggactc gcgcgattct gtgttttatc agaatatgcg ggaattgatg ccccggggga
     6721 tgaaggcgaa cagtcttgtg gtcggctatg atgcggacgg tctgccgcaa aaagtctatt
     6781 ggagtttcga caatggaaaa aaacgccaga gtttcgaata ttatttgaaa aacggaaatc
     6841 tttttattgc acaatcttcg acggtagcat tgaaagcgga tggcgtaacg gcggatatac
     6901 agacctatca tgcgcaacag acgtggtatt tggatggcgg gcggattgtc cgcgaagaga
     6961 aacaggggga cagactgcct gattttcctt tgaacttgga agatttggaa aaagaggtga
     7021 gccgttatgc agaggctgca gcgagacgtt cgggcggcag gcgcgacctt tctcactgaa
     7081 cgcaaatgcc gtctgaaacc ggttttcaga cggcattgga gagtcaaacg ctttttgcaa
     7141 tgccgtatat gccgaatccg attcgctaca aattgaaaac cacaatccaa aaaacggaaa
     7201 tacacattaa acggatcttt ttaccgaccg tatcatccca taggttttcc accccttttt
     7261 tcatccataa attcaaacca tttaagtttg catccttacc gacaattgcg gcagcattat
     7321 ccgcataacg gtcacccaaa ttagccaaat aacgccaacc gccataggta tcgccacgga
     7381 aagcataacc acgcaaggtt tggatttgtt ctggtgttaa aacaacttca ccctcattca
     7441 taataagatc ctttaaatta ttttttactt ggattgttta caaaactctg taccaattgc
     7501 tctacttgtt ggtgataacc taccaactcc tgctgtgtga gctgctgctt ttcccgaata
     7561 aacgggtaat actgtaccgt gaaaccgtta atacaggaac ctacgctagt gctataatta
     7621 tcttcttcag cctggtttac tcgattattc caatgagtaa taatcgctgg attttctcct
     7681 tttttaatat agtaaaattc atgaaaatat ggattttcag gaatatgtct tttatcttca
     7741 taagcctcgt attcaggata ggcagatggc atttttttaa ccccgtaagt aagcaatctt
     7801 tctcccatag tcgtgcttaa actacacgca tcttttcgga taacaatatc gtccacgcta
     7861 tccaaaccgt cgcgtagaaa ttggataaag tg
//
LOCUS       DAXHRI010000089         7892 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXHRI010000089 DAXHRI010000000
VERSION     DAXHRI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.089"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(15..605)
                     /locus_tag="ACFM75_001404"
     CDS             complement(15..605)
                     /locus_tag="ACFM75_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC6255938.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            complement(666..1247)
                     /locus_tag="ACFM75_001405"
     CDS             complement(666..1247)
                     /locus_tag="ACFM75_001405"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC6255939.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            1325..1558
                     /locus_tag="ACFM75_001406"
     CDS             1325..1558
                     /locus_tag="ACFM75_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255940.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            1735..2463
                     /gene="rpsB"
                     /locus_tag="ACFM75_001407"
     CDS             1735..2463
                     /gene="rpsB"
                     /locus_tag="ACFM75_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC6255941.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAIRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            2592..3446
                     /gene="tsf"
                     /locus_tag="ACFM75_001408"
     CDS             2592..3446
                     /gene="tsf"
                     /locus_tag="ACFM75_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC6255942.1"
                     /translation="MAEITAKMVADLRTATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            3663..4382
                     /gene="pyrH"
                     /locus_tag="ACFM75_001409"
     CDS             3663..4382
                     /gene="pyrH"
                     /locus_tag="ACFM75_001409"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC6255943.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            4566..5528
                     /gene="mafA"
                     /locus_tag="ACFM75_001410"
     CDS             4566..5528
                     /gene="mafA"
                     /locus_tag="ACFM75_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6255944.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            5568..7013
                     /gene="mafB"
                     /locus_tag="ACFM75_001411"
     CDS             5568..7013
                     /gene="mafB"
                     /locus_tag="ACFM75_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6255945.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            6979..7362
                     /locus_tag="ACFM75_001412"
     CDS             6979..7362
                     /locus_tag="ACFM75_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255946.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            7579..7892
                     /locus_tag="ACFM75_001413"
                     /pseudo
     CDS             7579..7892
                     /locus_tag="ACFM75_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT         2263 a         2021 c         1770 g         1838 t
ORIGIN      
        1 ttgaaaatac tgcctcacac ctgcacgcca taccctgcca acctgccggt caggatttcc
       61 ctgtttttgc accaatcttc cctcagcata ctgtacacga ccgtatcgcg cacactgccg
      121 tctttacgga gcatatgcat acgcagcacg ccgtcttttt ccgcacccag ccgttcgatg
      181 gcacgttgcg aggcaaggtt cagaatatcc gtgcgccatc ccacgcaacg gcaagccaaa
      241 acatcaaatg cgtaatccaa cagcatgatt ttgcaacagg tgtttatccg tgtccgccgt
      301 gccgatgccg cataccatgt gaatccaata tccaaacgcg gaatctgcgg ttcaaaatga
      361 taatacgccg ttgtcccgac caccctgccc gcctcttcat cgacaaccgc aaacgccaaa
      421 cgcgttgcca atgctgtccc gatatagtct gccaccctat ccggatgggg cgcggacgtt
      481 actcccagct tccaaacttc cccatcgcaa accgcctcgc gcaaacccgt ttcatgatgc
      541 acatccaacg gttcgagccg tacgccaccc aacgacaaga ccggcagtat tatcttttcc
      601 gacatccttt tctcccaata ttccaccttc agacggcatt tccgttcaaa atgccgtctg
      661 aacggctaaa aacacaatat ccccgcctcc gacacaaaac cgtccagcgg caggtcgtgc
      721 gcctcgaccg acagtctgtc caccaactgg caggcaaagc ccacgcccac ggtttttgcc
      781 tgcaaacggt atttcatcgc tgcaagcgtc gcatcgtaat agccgcctgc ctgccccaaa
      841 cggtagcctt cgcggtctat gccgacaagc ggaacgagca ataccgacaa accgtgcacg
      901 cggattttgc gccctgcaaa ctgcggcacg ttcaacctcg ccctgccccg tatgcgctcc
      961 cgttccattc cgctttcagg atacggcgta aaccacatcc gccgcgaacg cggttcgata
     1021 taaggcagat aaagttttgc gccgcgtttt tgcgccgcgc ggacaaagcc gtccaaacgc
     1081 aattccttgc ccatcggcca atacacgccg attttccgac cgtgcttgat ataacgtttg
     1141 agcaggcggt taatttttac cgttgccgcc gcccgcacgt cccgccccat ttgcgaacgc
     1201 cgcccgcgca attcgcggcg cagggcgcgt ttttcctcat tcctcatttc agacggcctt
     1261 tcaggattgc ggtagaatgt tgcgattata acgattttgt taacattcaa acaggacgca
     1321 cacaatgtgg cacatcgtcg ccatcggcta tctttttgtt gccgttatgt attccgccgc
     1381 gcagccgagt attgcgcgcg ccttgattta tttggttttt tgggcggtgc tgcccaccgt
     1441 gttcacggtc ttcgtcgtaa ccgtccgccg ccgcaaccgc ttgatgaagc aacaggaaca
     1501 ggcagaattc gaacagcagc gtgcacaacg gcaaaaagac agcggcacaa aaccctgaat
     1561 cccttttcag acggcctctt atccgctata atccgtcagt tttccatttc ggaaacactc
     1621 ttttattttt taaaacttat gcccactttc gccgaagggt gcttgacaat aggcgtcgcc
     1681 tatcaagttc tatgcgattg aatgtgtgcc cttaaccctt tcaaggaaat caaaatgtct
     1741 caaattacta tgcgtcagat gattgaagcc ggtgtccact tcggccacca aacccgtttc
     1801 tggaacccga aaatggcaca atacattttc ggtgcgcgca acaaaatcca tatcgtcaac
     1861 ctggaaaaaa ccctgccgat gttccaagac gcgcaagaag ccatacgccg tctggttgcc
     1921 aacaaaggta cagtattgtt cgtaggtacc aaacgccagg cacgcgacat catccgcgaa
     1981 gaagcgaccc gcgccggtat gcctttcgtc gattaccgct ggttgggcgg tatgctgacc
     2041 aactacaaaa ccgttaagca atccatcaaa cgcctggaag aaaaaaccgc agccttggaa
     2101 aatgctgccg aaagcggttt cagcaaaaaa gaaattctgg aaatgcaacg cgatgttgaa
     2161 aaactggaac gttctttggg cggtatcaaa aacatgaaag gcctgcccga cgcgattttc
     2221 gttatcgata ccggctacca aaaaggtact ctggtcgagg ctgaaaaact gggcatcccc
     2281 gttatcgccg tagtcgatac caacaacagc cctgacggcg tgaaatacgt catccccggc
     2341 aacgacgact ccgccaaagc catccgcctg tactgccgcg gcatcgctga cgcagttttg
     2401 gaaggcaaaa accaagcgct gcaagaaacc gtagccgctg cccaagaagc cgctgccgag
     2461 taatccggca aaccgaagag gggcgttatg ccccttttct caaatatgcc gtctgaacgt
     2521 ccgttcgcgg cacacgattc ccgaatgcgg aaaatccttt ccgtatttcc caaaaatcta
     2581 ggagattcaa aatggcagaa attactgcaa aaatggttgc cgacctgcgc actgctaccg
     2641 gcctgggcat gatggaatgc aaaaaagcct tggttgaagc cgaaggcaac ttcgacaaag
     2701 ccgaagaaat cctgcgtatt aaatctggtg cgaaagccgg taaactggct ggccgtactg
     2761 ctgccgaagg cgtattggct tacgcgatca acggcaatgt cggcgcattg gtcgaagtaa
     2821 actgcgaaac cgacttcgtt gctaaagacg cgggcttcgt agaatttgcc aacttcgttg
     2881 cgaaaactgc cgccgagaaa aaaccggctt ctgttgaaga actgagcgaa ctggttgaag
     2941 cagaacgtaa agccatcatc gctaaattgg gcgagaacat gtctgttcgc cgcttccaag
     3001 tgatcgacac tgccaaccaa ctggttgcct acatccacgg tgcattggct accgaaggcg
     3061 tattggttga gtacaaaggc tctgaagacg tagcacgcaa aatcggtatg cacatcgttg
     3121 ccgctaaacc acaatgcgta agcgaagctg aagtagatgc tgaaaccgtt gaaaaagaac
     3181 gccacatcta caccgaacaa gccatcgctt ccggcaaacc tgccgacatc gccgctaaaa
     3241 tggttgaagg ccgtatccgt aaattcttgg ctgaaatcac tctgaacggc caagcattcg
     3301 tgatgaaccc cgatcaaact gttgcccaat tcgccaaaga aaacggcact gaagtggtca
     3361 gcttcgtccg ctacaaagta ggcgacggta tcgagaaagc cgttgtcgac tacgcagccg
     3421 aagtagccgc agccgccaaa gtgtaaggca cttatgaaaa agaaagcacc tggattccaa
     3481 acgaatcagg gtgctttttt ttgagaaaac cgtttgcggt acctatttta agacgaccga
     3541 atattcagac cgtcttaaaa caaaacaata ataaaccgac acaccctatc attaatattc
     3601 cgaccgtttg aaattcagac gacccaactc cgaccgacga cattcaagaa agcaaggtat
     3661 ccatgacaca gcaaatcaaa tacaaacgcg tattactgaa actctccggc gaatccctga
     3721 tgggttccga tccgttcggc atcaatcacg ataccatcgt tcaaactgtc ggcgaaattg
     3781 ccgaagtcgt taaaatgggc gtgcaagtcg gtattgttgt cggcggcggc aatattttcc
     3841 ggggcgtatc cgcccaagca ggcagcatgg atcgcgccac cgccgactac atgggcatga
     3901 tggcgaccgt gatgaacgcg ttggcactca aagacgcatt tgaaacttta ggcatcaaag
     3961 cgcgcgtaca atccgcactg tctatgcagc aaatcgctga aacctacgcc cgccccaaag
     4021 ccatccaata tttggaagaa ggcaaagtcg tgatttttgc cgccggtacc ggtaacccgt
     4081 tcttcacgac cgacactgcc gccgcattgc gcggtgcgga aatgaactgc gacgtgatgc
     4141 tcaaagccac caacgttgac ggcgtgtaca ccgcagaccc caaaaaagac tcatccgcca
     4201 cgcgctacga aaccattact tttgacgaag ccttgttgaa aaacctcaaa gtgatggatg
     4261 ccaccgcttt cgccctctgc cgcgaacgca agctcaatat tgtcgtattc ggcattgcta
     4321 aagaaggctc gctcaaacgt gtagtaaccg gtgaaaacga aggtacgctg gttcactgct
     4381 gattgaccat agtgtcggca gatatagtcg catatgggct tcagacagcc atttattata
     4441 tggagattat agtggattaa atttaaacca gtacggcgtt gcctcgcctt gccgtactgg
     4501 tttaaattta atccactata tttacaattt tgatacaatt tgtttttcat caaaggagaa
     4561 aatctatgca agcacggctg ctgataccta ttcttttttc agtttttatt ttatccgcct
     4621 gcgggacact gacaggtatt ccatcgcatg gcggaggtaa acgctttgcg gtcgaacaag
     4681 aacttgtggc cgcttctgcc agagctgccg ttaaagacat ggatttacag gcattacacg
     4741 gacgaaaagt tgcattgtac attgcaacta tgggcgacca aggttcaggc agtttgacag
     4801 ggggtcgcta ctccattgat gcactgattc gtggcgaata cataaacagc cctgccgtcc
     4861 gtaccgatta cacctatcca cgttacgaaa ccaccgctga aacaacatca ggcggtttga
     4921 caggtttaac cacttcttta tctacactta atgcccctgc actctctcgc acccaatcag
     4981 acggtagcgg aagtaaaagc agtctgggct taaatattgg cgggatgggg gattatcgaa
     5041 atgaaacctt gacgactaac ccgcgcgaca ctgcctttct ttcccacttg gtacagaccg
     5101 tatttttcct gcgcggcata gacgttgttt ctcctgccaa tgccgataca gatgtgttta
     5161 ttaacatcga cgtattcgga acgatacgca acagaaccga aatgcaccta tacaatgccg
     5221 aaacactgaa agcccaaaca aaactggaat atttcgcagt agacagaacc aataaaaaat
     5281 tgctcatcaa acccaaaacc aatgcgtttg aagctgccta taaagaaaat tacgcattgt
     5341 ggatggggcc gtataaagta agcaaaggaa ttaaaccgac ggaaggatta atggtcgatt
     5401 tctccgatat ccgaccatac ggcaatcata cgggtaactc cgccccatcc gtagagactg
     5461 ataacagtca tgaggggtat ggatacagcg atgaagtagt gcgacaacat agacaagggc
     5521 aaccttgatt cacactacca taaccgcttg ctaccaagga aaacaaaatg aatttgccta
     5581 ttcaaaaatt catgatgctg tttgcagcag caatatcgtt gctgcaaatc cccattagtc
     5641 atgcgaacgg tttggatgcc cgtttgcgcg atgatatgca ggcaaaacac tacgaaccgg
     5701 gtggcaaata ccatctgttc ggcgatggtc gcggcagtgt taaaaatcgg gtttacgccg
     5761 tccagacatt tgatgcaact gcggtcagtc ctgtactgcc tattacacac gaacggacag
     5821 ggtttgaagg cattatcggt tatgaaaccc atttttcagg acatggacat gaagtacaca
     5881 gtccgttcga taatcatgat tcaaaaagca cttctgattt cagcggcggt gtagacggtg
     5941 gttttactgt ttaccaactt catcggacag ggtcggaaat tcatccggag gatggatatg
     6001 acgggccgca aggcagcgat tatccgcccc ccggaggagc aagggatata tacagctatt
     6061 atgtcaaagg aacttcaaca aaaacaaaga ctaatattgt ccctcaagcc ccattttcag
     6121 accgttggct aaaagaaaat gccggtgccg cctctggttt tttcagccgt gcggatgaag
     6181 caggaaaact gatatgggaa agcgacccca ataaaaattg gtgggctaac cgtatggatg
     6241 atattcgcgg catcgtccaa ggtgcggtta atcctttttt aatgggtttt cagggattgg
     6301 gagttggggc aattacagac agtgcggtaa acccggtaac ctatgcggca gcacggaaaa
     6361 ctttacaggg tattcacaat ttaggaaatt taagtccgga agcacaactt gcggccgcaa
     6421 ccgcattaca agacagtgct tttgcggtaa aagacagtat caactctgcc agacaatggg
     6481 ctgatgccca tccgaacatt acagctactg cccaaactgc cctttccgta gcagaggctg
     6541 caggtacggt ttggagaggt aaaaaagtag aacttaaccc gaccaaatgg gattgggtta
     6601 aaaataacgg ctataaaaaa cctgctgttc gccttatgca gactgtagac ggggagatgg
     6661 ctgggggaaa taagccaccg aaaccaagta cgcagcaaca ctctacacac tctgataaca
     6721 atataggctt acctgcccca tatgttaaac ctgatacatc tatttctccg acaggaacaa
     6781 tttatgtaga cagcaaagga aatgcaatac caacaccaat aggcggttat ataacaggtt
     6841 cgcctgatgc tcgttttatc caagtccgag atagtaatgg aaacttgact ggagccagaa
     6901 tagatgatgc tcatcatgca aatacccatc aagacccacg cgcacttaca ggacacgcac
     6961 atgtaccagg agttacaaat gatgacggaa ccccatggtt acccatcaaa taatttacct
     7021 gttaaaatca taaatgatat tatttatgca acccagttag ttgaagattt ggttttagga
     7081 aaaataaaaa ttgttgattt tttaaaatca tataacaatt tttattattg gcttggtttt
     7141 gatgagttac ctcaatctga gaaaataaaa tttctaaact atctcaatat attaagtatt
     7201 cataaggaaa tacaagataa aactgtgaat agggtttata ccgattgttt tgatattgat
     7261 aaattacatt cgcttggtag aattacaact aatgaatgta ttaatggtat aaaaaaaatc
     7321 tatcaaaaaa acaaattaga atttgacaat atactcaaat aactagccta tgttttgtaa
     7381 aataaaaata ttcttgctga gtgtagagcc agagataaaa agatttgata gtagtttgca
     7441 gttgtagaat tatataaaca agaaaatagt cagcagccgt aggtcagatt ctcgaatccg
     7501 acattttcca acagcggcat ttcggaaacg atagatgcgt caaatatttt tgtcggatac
     7561 aaatatccga cctacatctc tgcgcagcag actctacaag gtattaatga tttaggaaat
     7621 ttaagtccgg aagcacaact tgccgccgtg agcctattac aggacagtgc ttttgcggta
     7681 aaagacagta tcaactctgc cagacaatgg gctgatgccc atccgaatat aactgcaaca
     7741 gcccaaactg cccttgccat agcagaggcc gcaggtacgg tttggggcgg taaaaaagta
     7801 gaacttaacc cgactaaatg ggattgggtt aaaaataccg attataaaac acctgctgcc
     7861 cgacctatgc agactttaga tggggaaatg gc
//
LOCUS       DAXHRI010000090         7872 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXHRI010000090 DAXHRI010000000
VERSION     DAXHRI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.090"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(265..1650)
                     /gene="pntB"
                     /locus_tag="ACFM75_001414"
     CDS             complement(265..1650)
                     /gene="pntB"
                     /locus_tag="ACFM75_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC6255947.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1716..2099)
                     /locus_tag="ACFM75_001415"
     CDS             complement(1716..2099)
                     /locus_tag="ACFM75_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC6255948.1"
                     /translation="MTFAYWCILIAYLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2103..3644)
                     /locus_tag="ACFM75_001416"
     CDS             complement(2103..3644)
                     /locus_tag="ACFM75_001416"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC6255949.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTDNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAASAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            3934..4767
                     /gene="serB"
                     /locus_tag="ACFM75_001417"
     CDS             3934..4767
                     /gene="serB"
                     /locus_tag="ACFM75_001417"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC6255950.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIIEEGFSL
                     SEKDAALLGSRQIDHAVLSDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAVGDGANDIPMLKETGIGVAYRAKPKVRAAADVCINFGGLERVRGLF
                     R"
     gene            4831..6054
                     /locus_tag="ACFM75_001418"
     CDS             4831..6054
                     /locus_tag="ACFM75_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6255951.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGTDGVS
                     ISFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLTV
                     STSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIRIILLAVCMFALIGVISVWFP
                     EVLGNGKAGNQLTFSGLTGWQYALELAAVKWPVVLMALAAGAYVGLITPSMMLGSTVS
                     FAAAAAWNSVFPAMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6214..7794
                     /gene="purH"
                     /locus_tag="ACFM75_001419"
     CDS             6214..7794
                     /gene="purH"
                     /locus_tag="ACFM75_001419"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC6255952.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEIPLEAGANRFEFKRVGGGLLVQTPDIHR
                     ISRSDLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1745 a         2141 c         2219 g         1767 t
ORIGIN      
        1 caagtggaaa aatacttggc agattatcag gcacacttgg aaaaagtgtt cggctaatta
       61 aaaatccatc ttcaacacgg agatggattt tgtttgtttc gttgattttg tgtcagtttc
      121 agatgtaggt gcttattcgg acgggccgtc tgaaaatgtt tgccccaatg caaaaaaatc
      181 actgcaaacc ttcataaacg gggtttgcag tgattttttc aaatcaaaca gattgaaaac
      241 ctgcgccgaa ttgttcagac ggcattattt tttcagttcg gacagaatgt catctacggt
      301 tttcttcgca tctccgaaac acatcacgct gttttcgttg aagaacagtg ggttttgtac
      361 acctgcgtag ccggtattca tcgagcgttt gaagacgacg acttcttttg ccttccacac
      421 ttccaacacg ggcatacccg caatcgggct gttcgggtcg gtttgggcgg cggggttgac
      481 ggtgtcgttc gcaccgatga ccaagaccac atcggtttcg gggaagtcgt cgttgatttc
      541 gtccatttcc aaaacgatgt cgtaggggac tttggcttcg gcgagcagta cgttcatatg
      601 accgggcagg cggccggcga cggggtggat gccgaagcgt acttcggtgc cgtttttacg
      661 taaaagctcg gtgatttcgg caacggggta ttgcgcttgt gcgactgcca taccgtagcc
      721 cggggtaatg atgacattgt ttgcgccttt cagcatttcg gcaatatcgg cagctttgac
      781 ttctcggtat tcccctatct cttggctgcc ggaagataat gtgccgctgt cgctgccgaa
      841 accgccggca attaccgaga caaacgagcg gttcatggct ttgcacataa tgtaggacag
      901 aatcgcgccg cttgagccga ccagcgcgcc ggtaacgatg agcaggtcgt tggagagcat
      961 gaagcctgcc gctgcggccg cccagccgga gtaggagttg agcatggaca cgaccacggg
     1021 catatctgcg ccgccgatgg aggcaaccaa gtgccagccg aatgcgaggg caatcagggt
     1081 cataatcagc aggatgaagc cgctaccgtt aatgccgacg aatacgagca gcaacacaaa
     1141 cgatacggcg agcgccagtg cgttgagctt gtgtttggcg ggcagttgca gcgggctgct
     1201 gctgattttg ccgttgagtt tgccgaatgc gaccagcgag ccggtaaagg ttaccgcgcc
     1261 gatgaaaatg cccaaataga cttcgaccag atggatggtg tgcatatcgt gcgaaacgtt
     1321 gcccggctcg atatagctgt tgaagccgac caaaaccgcc gctaggccga cgaagctgtg
     1381 cagcagggca atcagttcgg gcatttcggt catttccacc tttttggctt tgtagatgcc
     1441 gattgccgcg ccgatgagca tggcgatgat gatccagccc agtccgtggg tattgtcgga
     1501 aaaaacagtt acaaaaaggg cgaccgccat accggcgata ccggaatagc agccctgttt
     1561 ggcggtttcc tgtttggaca gccccgccag tgagaagatg aataaaattg cggcaacgat
     1621 atacgccgct gttacgagtc ctgaagacat ggaaattctc cgattttcga tgatttgttt
     1681 ttaatgccgt ctgaactgtt cagacggcat ctgtttcaag cagccgcgac aaacagcccg
     1741 acggtgcagg caaatccgcc cgcccacatc agcgagcgca atgctgcttt gtctgcgatg
     1801 tagcaccaga taaaggcgag gcggaacagg atgaacaggc cggcaagcgt gttgacggtt
     1861 gcttgtccgg cattgccggt tgcgtgtgcc gtcaaaacgg cggcggcaaa cggtgcaaaa
     1921 gcttcaaaac cgttttgctg cgcagcgtgg gcacgggcgg ctgcgccttg cgtgtgcgcc
     1981 agaaaatcgc gcggattgtg gttgtcttta aaccggaatc cgcccgcttt tttggcatac
     2041 gccgcacaaa aaagcggcaa taggtaggca atcagaatgc accaataggc gaaagtcatg
     2101 gcttaccctt tcttaaacat attcagcata cgccgtgtta ccgcaaagcc gccgaagatg
     2161 ttgatgctgg caatcaggat ggcaacaaac gacagcagcg aaacaaagcc gttgccctga
     2221 ccgatttgca gcagcgcgcc gacgacgatg atgccggaga tggcattggt taccgacatc
     2281 agcggtgtgt gcagcgagtg gctgacgttc caaacgacat agtagccgat gacgcaggcg
     2341 aggacgaaca cgataaagtg gttcaggaat gctgcgggtg cgaccgcgcc gacccacagc
     2401 accaatacgg cggcgatgac ggcgggcgcg agttttttcc acagggaaac aggtttcggc
     2461 tcgggcttgg cggcagacgc ggctttttca gacggcgttt gctgcggctg ggcggaaact
     2521 tgaatcggcg gaggcgggaa ggtgatttcg ccgtcgtggg taacggtcat gttgcggata
     2581 atcacgtctt cgaagtccag cgtgatttcg ccgtctttgt tcgggcttaa cagcttggtc
     2641 aggttaacca agttggtggc gtaaagttgg gaagactgtc cggcaaggcg gtttgccatg
     2701 tcggtgtaac cgatgatttt cacgccgttg tcggttacgg acaattcgcc cgggcgggtg
     2761 agttcgcagt tgccgcccgt cgccgccgcc aaatcgacga tgacggagcc ggatttcatg
     2821 ctttcgacca tttctttggt aatcagcttg ggcgcgggtt tgcccggaat ggcggcggtg
     2881 gtgatgatga tgtccacttc tttcgcctgt tcggcaaata gcttcatctc ggcggcgata
     2941 aattcgtcgc tcatcacttt ggcgtagccg tcgccgctgc cgcccgattc ttgcgggaag
     3001 tcgagtttca ggaacttgcc gcccatcgat tcgatttgtt ccgccacttc caagcgggta
     3061 tcgaatgcgc gcaccactgc gccgagcgag tttgccgtac cgattgccgc cagacccgcc
     3121 acacctgcgc caatcaccaa aacctgcgcg ggcggcactt tgccggcggc ggtgatttga
     3181 ccggtgaaga aacggccgaa ggcgttggcg gcttcaatta cggcgcggta gccgctgatg
     3241 tttgccatcg aagacaaagc gtccaaagcc tgcgcgcggg aaatacgggg caccatgtcc
     3301 atcgccagcg cattgacttt cttggcgcgc aaggcttcga ccaaagcctc gttttggcgc
     3361 ggccacagga agctgacgat ggtttgacct tcgttcaaaa gcggcagctc gccttcggac
     3421 ggcgcgttaa ccttataaat caaagggcag gcccaaaccg ccgctttgtc ggcaacggtt
     3481 gcgcctgctg tttggtaagc ggcatcgtcc aaacttgccg ccaaacctgc accgctttcg
     3541 acaacggttt caaagcccag tttgcccagc agggcgacgg tggcgggcgt acaggcgacg
     3601 cgggtttcgc cggataatga ctcgcgtggg atgccgattt tcatctctga atcctttttc
     3661 gggttgttta tatgtatcgt gggttaaatt taaatcgggg cagggcggag caacgccgta
     3721 ccggtttaaa accgattcac ttcaaatgtt aatatatttt agataatccc cttataacga
     3781 attttcatca ggctggcaat agttgcggca ttttcccgtg ttgtccgaca catactgccg
     3841 tttgtttttg aagccgtttt ccgattgcag tcccgcccgc cgtaacattc tttgccgttt
     3901 cactatataa tccgcatttt ttgagccacc gccatgccgc aagccctcgt cctccaattt
     3961 ccctccgccg aagccctgcc ttccgacttc ccctcccgcc tgcccgaacc tgattgcgct
     4021 gatgaaaagc gtatgcgttt tatcattgaa gaagggtttt ctttaagcga aaaagacgcg
     4081 gcgttacttg gcagccgtca aatcgaccac gccgtgttgt cggatatgga ttttggcgaa
     4141 ctcggtttga ttgtcagcga tatggattcg acgctgatta ccatcgaatg cgtcgatgaa
     4201 attgcggcag gcgtgggttt aaaagacaag gtagcggaaa ttaccgagcg ttcgatgcgc
     4261 ggagagttgg atttcgagca gtcgttgcgc agccgcgttg cgctgttggc gggattggac
     4321 gaacagattt tggcggacgt ttatgaaaac gttttgaagc tctcgcccgg tgcggaattt
     4381 ttgttggacg aatgcaaaag gcacgatgtg aaattcctgc tggtgtcggg cggcttcacg
     4441 ttttttaccg aaaggctgca acaacgcctc ggcttcgaat accagcacgc caatgttttg
     4501 gaaattgaaa acggcaggct gaccggacgt ttgaaaggta gaatcatcga cgcgcaggca
     4561 aaggcggatt tattgcgcga ataccgcagc cgcctcggat tgcagccgca tcaggtgttg
     4621 gcggtgggcg acggtgcgaa cgatattccg atgctcaaag aaacgggcat aggcgtggct
     4681 taccgtgcca aaccgaaagt gcgggcagcc gccgatgtct gtatcaactt cggcggtttg
     4741 gagcgtgtac gcggcctgtt caggtaggcg gataggaaac ggatgtcgtc tgaaaggttt
     4801 tcagacggca tttgaacggc aggaacgaca gtgggacgca gaaaactttg gtttgccctg
     4861 gcagcagcag gcgttatcgg cggtttggtc ggcattgtgc tgacggaact gatgcacttc
     4921 atacaacata cggcatacgg ttatggcacg gacggcgtgt ccatctcgtt ccgcgaaggt
     4981 gtggcacagg cttccggtat gcggcgcgtt gccgtgctga cgctgtgcgg cgcggtcgca
     5041 ggcagcggct ggtggttgct gaaacgtttc ggcaagccgc aaatcgaaat caaagccgcc
     5101 ttgaaacagc cgttgcaggg gctgccgttt ctgacgacgg ttttccatgt tctgctgcaa
     5161 atcataacgg tcggactcgg ttcaccgctc ggacgggaag tcgccccgcg tgaaatgact
     5221 gccgcgttcg cttttgccgg cggcaggcgg ttgggtttgg atgaagacga aatgcggctg
     5281 ctgattgctt gcgcttcggg tgcgggtttg gcggccgtgt ataacgtgcc gctcgcctcc
     5341 acacttttta tcctcgaagc catgctgggc gtgtggacgc agcaagccgt cgccgccgca
     5401 ttgctgactt ccgtcatcgc caccgccgtc gcgcgcatcg gcttaggcga cgtgcagcaa
     5461 tatcatccgg ctaaccttac cgtcagcacc tcattacttt ggttttccgc cgtcatcggc
     5521 ccgatactgg gcgcaaccgc cgtctttttc cagcgcacag cccaaaagtt tccctttatc
     5581 aagcgcgaca atattagaat cattctcttg gcagtctgca tgtttgcgct catcggcgtg
     5641 atttccgttt ggtttcccga agttttgggc aacggcaaag caggcaacca actgactttt
     5701 agtggtttga ccggttggca atacgctttg gaacttgccg ccgtcaaatg gccggtcgtc
     5761 ttgatggcgc tggcggcagg cgcatacgtc ggactgatta cgccgtcgat gatgcttggc
     5821 agcaccgttt cttttgctgc cgccgccgcg tggaacagtg ttttccccgc aatgccgtct
     5881 gaaagcgcgg cagtcgtcgg agcggcggtg tttctcggcg tatcgctcaa aatgccgctg
     5941 accgccattg tgttcgtatt agaactgacc tacgcgccgc tgtccctgct gctgcctttg
     6001 tgcatcggca tggcgggggc attgggaaca tcgggaaaaa tgggcttcaa atgaatgtcg
     6061 acaaatgccg tctgaaaggt tttcagacgg catttctttg taaattgtca acaatatctg
     6121 caaaaatcta ctgccaaaaa actgaaaata gcggataata ccgccccgaa aaagttcccc
     6181 tttccccatc caactgtcag aaaaggaagc ccaatgtccg tcatcaaacg tgccctgatc
     6241 agcctgtccg acaaggcagg cgcggtcgaa tttgcccaaa ctctgcacaa gctcggtgtc
     6301 gaaatcctct ccaccggcgg cacggcgaaa ctgcttgccg atgcgggcgt tcccgtgatt
     6361 gaagtcgccg actataccgg cttccccgaa atgctcgacg gccgcgtgaa aacgctgcat
     6421 ccgaaaatcc acggcggtat tttgggtcgg cgcgatttgg acgaacacgt tgccaagatg
     6481 gaagaacacg gtatcggcaa catcgacctt gtgtgtgtca acctctaccc cttcgccgcc
     6541 accatcgcca aaccaaactg cacactggaa gacgcgattg aaaacatcga catcggcggc
     6601 ccgactatgg tgcgttccgc cgcgaaaaac tggaaacacg tcgccatcgt taccgacacc
     6661 gccgacttcc ccgccattgc cgccgaactc gaagccaaca acggcgcatt gagcgacaaa
     6721 acccgtttca acctctcacg caaagcattc agccacaccg cccaatacga cggcatgatt
     6781 tccaactacc tgacctccct ttcagacgac gttttgagcg gccagcccca aatcggcgaa
     6841 ttcccaagcc agttcaacca aagctggatc aaagtgcaag acatgcgcta cggcgaaaac
     6901 ccgcaccaac gcgccgcgtt ctaccgcgat attgaccccg ccgcaggcag cctttccgcc
     6961 tacaaacaac tgcaaggcaa agaattgtct tacaacaaca tcgccgatgc cgatgccgca
     7021 tgggaagccg tcaaatcctt tgacgcacca gcctgtgtga ttgtgaaaca cgccaatccg
     7081 tgcggcgtcg ccgttgcagc cgatacattg accgcctaca aactcgccta cgtcaccgac
     7141 accaccagcg cattcggcgg catcatcgct ttcaatcgcg aagtcgacgg cgaaaccgtg
     7201 aaacagatta ccgacaacca atttatggaa gttttgatgg cgccgaagtt taccgccgaa
     7261 gccctcgaaa tcgccgccgc caagaaaaac gtgcgcgtat tggaaatacc gctcgaagca
     7321 ggcgcgaacc gcttcgaatt caaacgcgtc ggcggcggat tgttggtaca aacccccgac
     7381 atccaccgca tcagccgctc cgatttgaaa gtcgtctcca aacgccaacc gaccgagcag
     7441 gagtggaacg atttgctgtt tgtctggaac gtcgcaaaat acgtcaaatc caacgccatc
     7501 gtcttcggca aaggcggtca aacctacggc attggcgcag gccaaatgag ccgcgtggac
     7561 agcacccgca tcgccgcccg caaagcgcaa gatgccggtt tcgacctcaa cggcgcgtgt
     7621 gccgcatccg atgccttctt cccattccgc gacggtgtgg acgtgattgc cgaacagggc
     7681 atcaaagcca tcatccatcc ggcaggctcg atgcgcgatc aggaagtttt cgacgcggcg
     7741 gacgaacacg gcatcgcgat ggtggtaacc gacgtgcgcc acttccgcca ttaacgcaga
     7801 tgaacaaggc aatgccgtct gaagggcttt cagacggcat tttgcgctat tttgtgaagg
     7861 tagggatgac gg
//
LOCUS       DAXHRI010000091         7804 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXHRI010000091 DAXHRI010000000
VERSION     DAXHRI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.091"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(97..777)
                     /gene="tsaA"
                     /locus_tag="ACFM75_001420"
     CDS             complement(97..777)
                     /gene="tsaA"
                     /locus_tag="ACFM75_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC6255953.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAKACIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            891..1527
                     /locus_tag="ACFM75_001421"
                     /pseudo
     CDS             891..1527
                     /locus_tag="ACFM75_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
     gene            1564..2544
                     /gene="waaC"
                     /locus_tag="ACFM75_001422"
     CDS             1564..2544
                     /gene="waaC"
                     /locus_tag="ACFM75_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC6255954.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREIGHLKQTLRQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFVQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRALLQKLNEEQQCNVYLTWGN
                     ETEKTRAEQIADGLPFAIVCDKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAVAKNLGNIGQIPTADLVYQTLMDCVAADEGSKVV"
     gene            2759..3508
                     /locus_tag="ACFM75_001423"
     CDS             2759..3508
                     /locus_tag="ACFM75_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC6255955.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            3519..4454
                     /locus_tag="ACFM75_001424"
     CDS             3519..4454
                     /locus_tag="ACFM75_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC6255956.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNMTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAEQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            4517..5128
                     /locus_tag="ACFM75_001425"
     CDS             4517..5128
                     /locus_tag="ACFM75_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC6255957.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(5180..5497)
                     /locus_tag="ACFM75_001426"
     CDS             complement(5180..5497)
                     /locus_tag="ACFM75_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC6255958.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            5655..6926
                     /gene="purD"
                     /locus_tag="ACFM75_001427"
     CDS             5655..6926
                     /gene="purD"
                     /locus_tag="ACFM75_001427"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC6255959.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDIISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVV
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            6960..7562
                     /locus_tag="ACFM75_001428"
     CDS             6960..7562
                     /locus_tag="ACFM75_001428"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6255960.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQAMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCLALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            7590..>7804
                     /locus_tag="ACFM75_001429"
     CDS             7590..>7804
                     /locus_tag="ACFM75_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255961.1"
                     /translation="MHTQRKNFSDSISLHDCYCTEIKVEGRNVCFNFEDGFVVPDNNP
                     NNQAGKHLKTDFSRVVLIHKNENAEDD"
BASE COUNT         2073 a         1523 c         2098 g         2110 t
ORIGIN      
        1 aatttaatcc gctataactt gatattgcgg cttatggcga acaaacaaca gccgtttgtt
       61 caactgctat gcggcaaaac cggatttttg cccgatttaa agcggggttg gggaaagatc
      121 aataacggtt gcacggtttt cctcgatttg aaatctgact tcgtaatctg caatattcat
      181 cacataaatc cgttcgggaa tattctgata ggcggggcgc ggatcttggg caatgctttg
      241 gctgataagg tttttggtgt ttgcagataa attttccgcg ccgatgtttt cctgccaaac
      301 gacttccaac tctacgggtt tgccgctgac gaaaccggat gcggcatcgg gtttggattc
      361 gacaaagggg atataaggtt tgatgtccac aatcggtgtg ccgtccagca ggtctgcgcc
      421 gctgcaatag aggcggacgg gtttgccggt ttcgatgcgt tcgagtttca ggagcgagag
      481 tccgagatgg ttggggcggt gggggctgcg cgtggcgaac acgcccattt tttgtttgcc
      541 gccgagccgt ggcgggcgca ccatttgcgc ccagccttca tccaatacgc cgtgaaaaat
      601 aaaacttatc cacacataat cgaaatcttc cagcccgcgc acgctgtctg cggtgaattt
      661 gggattcagc tcgatgcagg cttttgcggc ggagaccaaa ccgggctggc gggcgatgcc
      721 gaatttctgt ttgtagggcg agcgggcggt gccgatgggg gtaatggtat aagtcataag
      781 gttgtgggta agttttttgg aaaaacctta tcttatcaca atcggtttta ccgcttttgg
      841 gcagtaattt gttataatac agggcatttt aatcgcgttg aaataatgag atgattgttt
      901 ccattgatgt tgatgcacaa aaaactttta cgcctttgtg tcctgacgag ctgcctgtga
      961 acgaggggca tttgattgtt gaagagttga atgcccaagc cgctttggcg gatttgcgcg
     1021 tgatgacgaa agatgcgcat catatggcgg cgaaatggct tgtggataac cctgttgata
     1081 tgttgaagcc gacaggtttg cctgatgcgg atttgacttg ggtggctcat gcaatgatcg
     1141 gtacgcacgg ctatgaattg ttggacgggc tgccttctgc taaagaatac gattattgcg
     1201 tttggaaagg tgttgatccc gaattgcatc cttacggcgc gtgttttcac gatattgaag
     1261 aaaaactgag cacggggttg attgaatggc tgcgttgtca acatacggat acggtcattg
     1321 tcggcggatt ggcaacggat tattgtgtta aaacaacggt tttgcagttg ctcaaaggcg
     1381 gcaattggca ggttatcgtc aatgaagcgg cttgtcgggg cattgcgccg gaaaccatcg
     1441 aaacggcatg gcaggaaatg cgttctgccg gtgcaattat cttaaaaaac gctgaagaaa
     1501 ttaaaaaaat atattaataa tcaataagtt aaaattgttt cacgtgaaac acacttctta
     1561 aatatgaaaa ttttgcttgt ccgcttgtcc agtatgggcg atttaattca cactttgccc
     1621 gcaatcgaag atttggcgcg acaatgccct gatgtggaac tccattggtt gtgtgaagcc
     1681 ggatttgcgg atattgcgcg cctgcatccg ttcgtaaaaa aaatccatgt gatgaaatgg
     1741 cggcaatggc gaaaacatct ctttcaggct gaaacttggc gggaaatagg tcatctgaaa
     1801 cagactttgc ggcaggaagc atttgatttt gtattggaca gtcaaggtct gattaaaagc
     1861 gcgtgtttcg ccaaaatggc aaaatcccct atttatggtt tagataaaaa cagcgcgcgt
     1921 gaggggttgg ctgcgttggc gtatgcgaaa acatacgctg taccgaaagg gaaaaatgcc
     1981 gtttggcgca accgtgagct gtttgtccaa gtatttggat atgcaatgcc ggaaacgcag
     2041 gtatttggct tgactgttcc tgaggcaggc cgtctgaaaa atttagcgca gccgtattat
     2101 gcggctttgc atgcgaccag tcgggacagt aagttatggc ctgtggaaaa ctggcgggcg
     2161 ttgctgcaaa agctgaatga agaacaacaa tgcaacgttt acctgacatg gggaaatgaa
     2221 actgaaaaaa cgcgtgccga acaaattgca gacggactgc catttgccat tgtgtgcgat
     2281 aaaatgaatt tattgcaggc ggcgtatctg ctgaaacacg cggtcggaat tgtcggtgtg
     2341 gataccggtt tgctgcattt ggcaaatgct ttggaaaaac ctgtggtcgg tatttatacc
     2401 gatactgatc cgattaaaac aggcgttcaa gtttcggctg ttgcaaaaaa tttgggcaat
     2461 attgggcaaa ttccgactgc agatttggtt tatcaaacgt tgatggattg tgtggctgct
     2521 gatgaaggct caaaggtcgt ctaaaattga tatagcggca tgatattttc caatttgtta
     2581 gatgcaggaa tgtatatcta ccagcctgag taaatgcttt aaaaatggta taatcggcaa
     2641 gcattatccg aaagattttt gaatgaattc gtttttaagg aaaacggatt tggttttata
     2701 gggaagaaag tttggtttcg ctggatggtg aaagcagcgt tctttttagg agggttcaat
     2761 gaaagcactg gtcgcagtaa agcgcgtagt ggactacaac gtcaaagttc gtgtaaaagc
     2821 cgatggttcg gatgtggata tcggcaatgt caaaatgtcg atgaatccgt ttgacgaaat
     2881 cgctgtggaa gaagctgtcc gtttgaaaga agccggaaaa gtaagcgaaa tcgtagcggt
     2941 ttctttgggt gagaaaaaat gcgaagaaac cttgcgtaca gctttggcga tgggtgccga
     3001 ccgtgccatt catgttgaaa ccgatactaa actggagtct ctggccgttg ctaagttgct
     3061 gaaagccgtt gcggacaaag aaaatccgca aattttcttt ttgggcaaac aagcgattga
     3121 tgatgatgcc aatcaagtgg cgcaaatgct ggcagctttg ctgaatgcgg cgcaaggtac
     3181 gtttgcttca aaagtacaca ttgaaggtgg tgaagtacag attgtgcgcg aaattgatgg
     3241 cggcgaagaa accatagcat taaaactgcc tgctgttatc agtgctgatt tgcgtttgaa
     3301 cgagccgcgc tttgtcaaac tccccaatat tatggcggca aagaaaaaac ctttggaaaa
     3361 actgactcct gacgatttgg ttgccgacat ttcacctcgt ttgaaaacgg tgaaatttgc
     3421 cgaacctaag gcgcgtcagg caggcgtaaa agtagcaagc gttgccgaat tggttgaaaa
     3481 attgaaaaac gaagccaaag tgatttgagg aggctgaaat gagtgtattg attattgtcg
     3541 aacacgacaa caaacagttg aatcctacca ctttgcatgc tgttaccgct gccgctaagc
     3601 tgggtaaaat cgatttattg gttgccggaa gcggtgcatc tgctgtagta gagttcgcaa
     3661 aacaagtggc gggtgtggaa aaagttttgg ttgcagatgc tgcccattat gccgaaggtt
     3721 tggctgaaga gttggctccg ctggttgtca aattggcagc ggattactgc tatgttgcag
     3781 caacggcaac tacattcggt aaaaaccttt tgcctcgcgt agcagcctta ttagacgtac
     3841 cgcaaatttc tgatttgacc gagattgtgg ataacatgac ttttgtgcgt ccgatttatg
     3901 cagggaacgc atttgaaacc gtgcaagctg attcagaaaa attggtgctg accttccgtg
     3961 cgacggtttt tgacgcagtg gcggagcaag gtggtaatgc tgaggtaatt aatgttgaag
     4021 caacccctgc ccaaaacctg agtcgttttg ttaaccgtca gctttcccat tccgatcgtc
     4081 ctgaactgac tcaggcaaaa gtgattgttt ccggtggtcg tgcgttgggt agtgcggaaa
     4141 aatttaatga agtgctgaca ccgttggcgg atgttttagg tgcggcaatc ggtgcatccc
     4201 gtgcggcggt tgatgccgaa tatgcgccaa acgatgctca agtcggacaa accggtaaag
     4261 tggttgcgcc gcaactctat tttgcaatcg gtatttcagg tgcgattcaa cacgttgctg
     4321 gtatgcaaga cagtaaggtg attgttgcaa tcaataaaga tgctgatgcg ccgattttca
     4381 atgtagccga ttacggattg gttggcgatt tatttgaaat cgttccacaa ttaacagaag
     4441 cattgaaaaa ttaatgtttc acgtgaaacg ttcagacgac cttgttatta atgaggtcgt
     4501 ctgaaaacat ttcagcatga ttacgactta taaaaccatt acttcctcga cgcaggctga
     4561 gtttaaagat aaaggcagcc gttttattgc atttgcctat ccgattcgga cattggctga
     4621 tgtgaaaaaa tacctcgatc cgttaaagga agagcatcat aaagcacgac actggtgcta
     4681 tgcctatcgt ttgggtgtgg atggtgtgca atttcgtgcc aacgatgatg gagagccatc
     4741 aggaagtgct ggacgaccaa ttttgggaca gattgattcg gtgggtatta ccgacgtttt
     4801 ggtcgtggtc gtccgctatt tcggtggtac tttgttaggt gttccaggtc tgatacatgc
     4861 gtacaaagag gcgacggctc aagcgttggc agttgcagaa gtagttgaaa agaatattga
     4921 aaaaactgtt tggctgaaat gtgaatatcc gtttttgaat gaagcgatac gcattgctaa
     4981 acaatatcag gcggatgtat tggagcggga tttgcagttg gattgccgat tgacggtcag
     5041 tttgtcgttg gtaaattatg aggcctgtgt tgcggcttgg aagagtacac ggcagattga
     5101 agtaaatacg gaaaagcctt ttgaatgaaa ggtcgtctga aatgctttgt gatggtattt
     5161 cagacgacct cttcgctgac taatcttctt gtgtatattt gtctaaggca tcgacgctgg
     5221 agcgcaaata taaccaagcc agttgatgga attcgcccag cgcgttccat agcgcatctt
     5281 cactcataat gctgtattcg ccttctgtgc gcaaatccac ctccgctcca tcttcaatag
     5341 cccgatgaca agcctgatat tcgtgggtat aaaaattgtc catatcgcga atcaaagcga
     5401 cggcatgttt ccaacggcgg atgtcttctt ccaattgcgg tagtaaatga caccattcgc
     5461 ggtatttgct ttggatttca tcaatacgtt tttgcatggt tgcttgtcct tttttaattt
     5521 atgaataagt tgaataggtt gcagagtgga atgaagcggt cttttgtttg attctactaa
     5581 aatttcttca gatgggataa aagtcccttt gtatcgagtt ttcgttaaaa gtacatattt
     5641 gtcaaggaga tggcatgaaa ctgctggtta tcggtaatgg cggtcgcgaa cacgcgctgg
     5701 cttggaaatt ggcgcagtcg cctaaagtgg aaacagtatt tgttgcgcct ggtaatgccg
     5761 gtacagcgat tgaacccaag ttgcaaaaca tcgacttgac tgcgcatcag gatttgattg
     5821 aattctgtcg taaagaaaat attgttttta ccgtcgtcgg ccctgaagcg cctttggcgg
     5881 caggtgttgt ggatgatttc cgtgccgcag gactaaaaat attcggtccg acacaatatg
     5941 cggcgcagtt ggaaagttcc aaagatttcg ccaaagcatt tatggcgaaa tacaatattc
     6001 caaccgcgca atatcaaacc tttgaaaacg ccgatgccgc acatgattac gtcaatcaga
     6061 aaggtgcgcc tatcgttatc aaagccgatg gtttggcggc aggtaaaggc gtgattgtgg
     6121 cgatgacttt ggatgaagcg catgctgcga ttgatgatat gctgctggac aacaaaatgg
     6181 gcaatgccgg cgcgcgtgtt gtgattgaag atttcctgca aggcgaagaa gcgagtttta
     6241 tcgtcatggt tgatggcaat aatgtactgc ctatggcaac cagccaagac cacaagcgtc
     6301 ttttagacgg cgacaaaggt ctgaatacgg gcggtatggg tgcgtacagc cctgcgcctg
     6361 tggtaacgcc tgttgtgtac gaacgcgcga tgaatgaaat tattttgccg accgtagcag
     6421 ggatgaaagc ggaggggcat gagttcaccg gtttcctgta cgcaggtttg atgattgatc
     6481 aaagcggtgc accccatacg attgagttta actgccgttt cggcgatcct gaaacccagc
     6541 cgattatgag ccgactgaac agcgacttat cggatttggt tgaagcggca atagacggca
     6601 aactcgatag cgtaaccgcg gaatggagcc cgcaaactgc cgtgggcgtg gtactggcgg
     6661 cgcaaaatta ccccgaaacg cccaaaaaag gcgatattat ttccggtctg gatgctgcca
     6721 accaagtagg caaagttttc catgcaggta caacggcaaa cgagaaaggt gacgtattga
     6781 ccaacggcgg acgcgtattg tgtgttgtgg gattgggcga caatgtggtg caagcgaaag
     6841 ctaaggcata tggcgcgttg gaaaaaatca gtttcgacgg gatgcaatac cgtaaagaca
     6901 ttgccgataa agcgattaac cgttaattag aaagaggatg atgccgtctg aagccaagta
     6961 tgactgatgt gcttcagacg gtattttata ttatgttatt tccgagaatt atcgcagcat
     7021 cagcgcaacg taagttaagc gtttatttga aaaaaggcgg tttggtcgct tatccgaccg
     7081 aatcttgtta tggcttgggg tgtcttccga cattagccaa agcactcggt aagttggcgc
     7141 atttgaaaaa aaggccgcaa cacaagggta tgattgtcat cgggaatcaa tttgaacagt
     7201 tgcagccttt acttcaaatg ccgtctgaaa atctgcaggc tatgcttaga aaagaatggc
     7261 ccgcccctaa aacattccta ttatctgcca aatcctgcgt attacctgag ttacgcggga
     7321 aacagcgcag caagttggcg gtcagggttc ccgcccatgt aggtgcgcgc cgcttgtgtc
     7381 tggctttgca aacgcctttg gtctcaactt cgtgcaaccg cgcaggcaaa cgcgcgtgtc
     7441 ggacggagag ggaagtcaga aggcaattcg gacgcgatgt gtggattgtt ggtgggcgaa
     7501 tcggcaggca gaaatcgccg agtcagatta ttgacgggga aacaggtaaa cgtttgcgtt
     7561 agatatgggt acttgaatga aaactttttt tgcatacaca aagaaaaaat ttttcggata
     7621 gcatttctct acatgattgt tattgcacag agataaaggt agaaggacgc aacgtctgct
     7681 ttaattttga ggacggtttt gtcgttccgg acaataatcc gaacaatcaa gcgggcaagc
     7741 atctgaaaac agatttttcc cgtgttgttt taatccataa aaatgaaaac gccgaagatg
     7801 atta
//
LOCUS       DAXHRI010000092         7658 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXHRI010000092 DAXHRI010000000
VERSION     DAXHRI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.092"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(9..425)
                     /gene="dksA"
                     /locus_tag="ACFM75_001430"
     CDS             complement(9..425)
                     /gene="dksA"
                     /locus_tag="ACFM75_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC6255962.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(572..1381)
                     /gene="proC"
                     /locus_tag="ACFM75_001431"
     CDS             complement(572..1381)
                     /gene="proC"
                     /locus_tag="ACFM75_001431"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC6255963.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLQKELGVETSETLPELYSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTL
                     SRYLGGTRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVTLAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1412..1819)
                     /locus_tag="ACFM75_001432"
     CDS             complement(1412..1819)
                     /locus_tag="ACFM75_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC6255964.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIRKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1819..2505)
                     /locus_tag="ACFM75_001433"
     CDS             complement(1819..2505)
                     /locus_tag="ACFM75_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC6255965.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2625..3668
                     /gene="pilT"
                     /locus_tag="ACFM75_001434"
     CDS             2625..3668
                     /gene="pilT"
                     /locus_tag="ACFM75_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC6255966.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3831..5057
                     /locus_tag="ACFM75_001435"
     CDS             3831..5057
                     /locus_tag="ACFM75_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6255967.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5343..7493
                     /gene="yccS"
                     /locus_tag="ACFM75_001436"
     CDS             5343..7493
                     /gene="yccS"
                     /locus_tag="ACFM75_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC6255968.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGTYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPTKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALNTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1892 a         2547 c         1663 g         1556 t
ORIGIN      
        1 tgccgccatc agtcggcaaa ctgttttttc atcctctctc ggcgttcttg ggactcaaca
       61 gataaagtgg ctgtcgggcg tgccagcagc cgcttcagac cgataggctc tcccgtatcg
      121 gcacagaatc cataatcccc ttcatcaata ttgcggatgg tcgcctgtat tttactgaga
      181 agttttcgtt cccgatcgcg ggtacggagt tccaatgcgt actcttcttc ctgtgtggca
      241 cggtcggcag gatcgggggc tgattcgtgt tcttggagat gccctgtcgt agcggaagca
      301 ttttcgatga gttcgtcttg catttttacc agcaattcgc ggaaaaaagc caaatgctcg
      361 tcattcatat aatcgtcttc cggcccgttc caattcaaaa tatcttgttc tgtcagcttt
      421 gccataatgt atatcctctc ggttcaccga aaaaaacaaa gcaccacaaa gtggctttat
      481 tcctttgatt tttataaggc ttattgccta aggcgttttt gaaaacgcgc atcataacac
      541 gttttggatt ggttttttta ttttctttac attattgata ttgccgttcc atttcctgcg
      601 aacggcgcac acaggcacaa acgccctcac ttatggcttc ggcgacacga tgccgcctga
      661 aggcttccac ggcttcgtgg gttgtcccgc cttttgacgt tacatttttt tgaagcttct
      721 cgaaatcttc acccgtctgc tcggcaaggg taaccgctcc tttaaacgtt gccagactga
      781 gcgcgcgtgc ttctgccata tcaaaccctt gtcggatggc ggcattttgc aatgcgtcca
      841 gcagataaaa cacatatgcc ggcccgctgc cgctgatacc ggtaatgctg tgcatttgcg
      901 cttcatcttc caaccaaaca gtcaaaccga ctgatttcat gattcgatcg gcaatcctgc
      961 ggtctgtttc cgatacttcc gcttcggcat acataccgga tacgcccagc ccgatttttc
     1021 cgggtgtatt cggcataacc cggacaatgc ggcgtgttcc cccgaggtaa cggctgagcg
     1081 tatcgaccgg caatccggct gcgacagaaa gcaccaatgc gccgttggtg cggatatttt
     1141 tgcacgcggc ttccatatcc tgcggcttga cggcaaggat taaaacatcg tcggaataaa
     1201 gctccggcaa ggtttcagaa gtttcgacac ccaactcttt ttgcaaacgt tcgcgctttt
     1261 ccgcaccccg gttggcaata tggatgcggt aaccgccttg tttgaccaat ccgcccgcaa
     1321 cggcagccgc catattgccg ccgccgagaa aataaacatt cattatttct accctattca
     1381 aaattacctg actaaattca ttcccaattt tttaccatcg gacgcgctcg gacagaccgt
     1441 tgcgccgcaa cccctgcttt ttcaaacaat caaaaccttc ctgtttgaac gtctcaatca
     1501 aaggtttgcc gtcacgcgta cgccaatcca acccgcaggc ttcaaacttc cttattttcc
     1561 tgtaaaagta agattgttgt gcggattcat attcatttgc cctgacggaa cgattgccgt
     1621 ctgaaagcgg tatctcgtag tcctctaaag acggcggact ctcgccgcca atatcccaaa
     1681 aatcgatggc ggcaggtttt ctcggcttac accagccgga cagggacgaa cactcatacc
     1741 aacctgccaa ccggcagcct gcaaccgtaa atgcaagcaa cgcagccatt gccgtatatt
     1801 ttattgcccg aatttccatc agcccctttt cccgaaaatc gcgctgccga tacggacgtg
     1861 tgtcgcaccg cactcaatgg cggcaggcat atcgtccgac atccccatag acagcacgtc
     1921 agccttaacg ccagccgtat tgaggtcggc aagcagtttc cgcatcgttt gaaattgcac
     1981 cttcaactcc gtttcactgc tgttggcttt ggcaacacac atcagtccgc gtacgacgat
     2041 attcggcagc ttcgccactt ccacagcaag cgcgactgct tcttcgggcg cgacaccgtg
     2101 cttcaccgcc tcgcccgcaa tattcacctc gatacacacc tgcaagggcg gcattgagga
     2161 aggacgctgt tcgctcaacc ggacagcggt tttcagacgg catacggtat gcacccaatg
     2221 cgcgcgttcg gcgacaaact tggttttgtt ggactgcaca tcgccgatga cgtgccacac
     2281 gatgtcggtc aaatccgcca actcttccgt tttgccgtac cactcctgaa tatagttctc
     2341 gccgaaatca cgctgtccgg cggcgtaaac ttcgcggatg ccgtctgaag ggaaagtctt
     2401 accgacggca atcaggctga cggaatgcgg cttccttccc gcctgcagaa ccaattttcc
     2461 gatacggtcg gacacctcac aataacgttc ttgcaacacc gtcatagatt atcccctaat
     2521 taaaaatgat taaacagttg aaacccctcc agtcagggcg gtacaatcaa ggttgttaga
     2581 accattccaa ccaatcgaaa cattatacta aacagagccg cattatgcag attaccgact
     2641 tactcgcctt cggcgctaaa aacaaagcat ccgaccttca cctgagttcg ggcatatccc
     2701 ctatgattcg ggttcacggc gatatgcggc gcatcaacct tcccgaaatg agcgcggaag
     2761 aggtcggtaa tatggtaact tcggtgatga acgaccacca gcggaaaatc taccagcaaa
     2821 acttggaagt tgacttctcg ttcgaactgc ccaacgtcgc ccgattccgc gtcaacgcct
     2881 tcaacatcgg ccgcggtccc gccgccgtat tccgcaccat tcccagcacc gtcttatcgc
     2941 tggaagaatt gaaagccccg agcattttcc aaaaaatcgc agaatcgccg cgcggcatgg
     3001 tattggttac cggccctacc ggttcgggca aatcgaccac gcttgccgca atgatcaact
     3061 acatcaacga aacccagccg gcacacatcc tgaccatcga agacccgatc gaattcgtcc
     3121 atcaaagcaa aaaatccctg attaaccagc gcgagctgca ccagcacacc ctcagcttcg
     3181 ccaacgcgct gcgttccgca ttgcgcgaag accccgacgt tatccttgtc ggcgagatgc
     3241 gcgacccaga aaccattggc ttggcactga ccgccgccga aaccggacac ttggttttcg
     3301 gcacgctgca cacgaccggc gcagcaaaaa ccgtcgaccg tattgtggac gtattcccgg
     3361 cgggagaaaa agaaatggtg cgctctatgc tgtccgaatc gctgaccgcc gtcatctccc
     3421 aaaacctgct gaaaacgcac gacggcaacg gccgtgtcgc ctcgcacgaa atcctgattg
     3481 ccaaccccgc cgtccgcaac ctcatccgcg aaaacaaaat cacgcagatt aactccgtcc
     3541 tgcaaaccgg gcaggcgagc ggtatgcaga caatggacca atcgctgcaa tcgctggtgc
     3601 gccaagggct gatcgcaccg gaagtcgcac gcagacgcgc gcaaaacagc gaaagtatga
     3661 gtttctgaca cacaaccgcc ttccggccat accggcggga aaacaaggcg caaacacgcg
     3721 gggcgggacg cagcatcccg cccggctacc tttccgaaca agaagcgtcc gccttcctgt
     3781 tgaaacctgc cgccgcaaac tgcaaggctt aaaccgaaaa gaagctaacg atgaataccg
     3841 ataacctgca cgacatcttg gacgaaatgg ttcaagtgta ttctcaaaaa aaacaaagcc
     3901 gatccgaaac cccggccgaa atcggcgcac acttccaccc gctgctcgac cgcctgtgcg
     3961 aaaccgcaga agcacaaaac gcgtccgaca tccttatcag caaaggattc ccgccctcgt
     4021 tgaaaatcaa cagcgcatta accccgcagc cgcaaaaggc gctgacaggc gaggaaaccg
     4081 ccgccatcgc cgcatcgacg atgaacgccg aacaatcgga aatattccgg cgcgacggcg
     4141 aaatcaacta ctccgtccag tcgcgcagcg gcacgcgcta ccgcgccaac gcctaccaca
     4201 gccaaggcag cgcaggtttg gttttgcggc gcatcaacca cgtcatcccg caaatgcagg
     4261 aattgggcct gcccgaaaaa ctcaaagacc tcgccgtcgc accgcgcggg ctgctgatta
     4321 tcgtcgggcc taccggttcg ggcaaatcca ccacgatggc gactatgctc gaacaccgca
     4381 acaaaaccct gcccagccat atcgttacca tcgaagaccc gattgagttt atctacaaac
     4441 cgcgccgctg catctttacc cagcgcgaaa tcggcgtcga caccataaac tggcagacgg
     4501 cggtacaaaa cgctatgcgc caatcccccg acgtggtctg catcggcgaa gtccgcagca
     4561 gggaaagtat ggaatacgcg atgcagctcg cccaaaccgg ccacctgtgc atttttacgc
     4621 tccacgccaa caccgcgccg cagtcgctcg aacgcatact caacttctac cccaaagaac
     4681 agcacaacca aatactgatc gacatcgccc tcaacctgac cggcatcatc tgccaacgcc
     4741 tcgccctcaa acaagacaaa acgggcagga cggcggttgt cgacttgctc atcaacacgc
     4801 ccgccatcca agacttcatc ctgaagggcg acctgatgaa catcagtaaa atcatggaaa
     4861 ccgccaaaac cgacggaatg cagacgatgg atcaaaacct tttcgaactg taccgtcacg
     4921 gcatcatcag ttacgaagaa gccctgcgcc agtccgtttc cgccaacaac ctgcgattgc
     4981 acatccaact gcacaaagaa ggcaaaacgc ccgaactcct ttacgacagg gtcaacggtc
     5041 tcaacctcat ttcctgatcc gcaaaaccca atgccgtctg aaaaccgcat ccccgttttc
     5101 agacggcatg attttatccg ccccattcat gtgctacact ttatagtgga ttaaatttat
     5161 agtggattaa caaaaatcag gacaagacga cgaagccgca gacagcacaa atagtacgga
     5221 accgattcac ttggtgcttc agcaccttag agaatcgttc tctttcagct aaggcgaggc
     5281 aacgccgtac tggtttttgt taatccacta tattcacttc ataacaataa accggtaaaa
     5341 ccatgaaaac cccactcctc aagcctctgc tcattacctc gcttcccgtt ttcgccagtg
     5401 tttttaccgc cgcctccatc gtctggcagc taggcgaacc caagctcgcc atgcccttcg
     5461 tactcggcat catcgccggc ggccttgtcg atttggacaa ccgcctgacc ggacggctga
     5521 aaaacatcat cgccaccgtc gccctgttta ccctctcctc gctcacggca caaagtaccc
     5581 tcggcacagg gctgcccttc atcctcgcca tgaccctgat gaccttcggc tttaccattt
     5641 taggcgcggt cgggctgaaa taccgcacct tcgccttcgg cgcactcgcc gtcgccacct
     5701 acaccacact tacctacacc cccgaaacct actggctgac caaccccttc atgattttgt
     5761 gcggcaccgt actgtacagc accgccatca tcctgttcca aatcatcctg ccccaccgcc
     5821 ccgtccaaga aagcgtcgcc aacgcctacg acgcactcgg tggctacctc gaagccaaag
     5881 ccgacttctt cgaccccgat gaggcagcct ggataggcaa ccgccacatc gacctcgcca
     5941 tgagcaacac cggcgtcatc accgccttca accaatgccg ttccgccctg ttttaccgtt
     6001 tgcgcggcaa acaccgccac ccgcgcaccg ccaaaatgct gcgctactac ttcgccgccc
     6061 aagacataca cgaacgtatc agctccgccc acgtcgacta ccaagagatg tccgaaaaat
     6121 tcaaaaacac cgacatcatc ttccgcatcc accgcctgct cgaaatgcag ggacaagcct
     6181 gccgcaacac cgcccaagcc ctgcgcgcaa gcaaagacta cgtttacagc aaacgcctcg
     6241 gccgcgccat cgaaggctgc cgccagtcgc tgcgcctcct ttcagacagc aacgacaatc
     6301 ccgacatccg ccacctgcgc cgccttctcg acaacctcgg cagcgtcgac cagcagttcc
     6361 gccaactccg acacagcgac tcccccgccg aaaacgaccg catgggcgac acccgcatcg
     6421 ccgccctcga aaccggcagc ctcaaaaaca cctggcaggc aatccgtccg cagctaaacc
     6481 tcgaatcagg cgtattccgc catgccgtcc gcctgtccct tgtcgttgcc gccgcctgca
     6541 ccatcgtcga agccctcaac ctcaacctcg gctactggat actgctgacc gcccttttcg
     6601 tctgccagcc taactacacc gccaccaaaa gccgcgtccg ccagcgcatt gccggtaccg
     6661 tactcggcgt aatcgtcggc tcgctcgtcc cctactttac cccctccgtc gaaaccaaac
     6721 tctggattgt catcgccagt accaccctct ttttcatgac ccgcacctac aaatacagtt
     6781 tctccacctt cttcattacc attcaagccc tgaccagcct ctccctcgca ggtttggacg
     6841 tatacgccgc catgcccgtg cgcatcatcg acaccattat cggcgcatcc cttgcctggg
     6901 cggcggtcag ctacctatgg cccgactgga aatacctcac gctcgaacgc accgccgccc
     6961 ttgccgtatg cagcaacggc acctatctcg aaaaaatcac cgaacgcctc aaaagcggcg
     7021 aaaccggcga cgacgtcgaa taccgcgcca cccgccgccg cgcccacgaa cacaccgccg
     7081 ccctcagcag caccctctcc gacatgagca gcgaacccac aaaattcgcc gacagcctgc
     7141 aacccggctt taccctgctc aaaaccggct acgccctgac cggctacatc tccgccctcg
     7201 gcgcataccg cagcgaaatg cacgaagaat gcagccccga ctttaccgca cagttccacc
     7261 tcgccgccga acacaccgcc cacatcttcc aacacctgcc cgaaaccgaa cccgacgact
     7321 ttcagacagc actgaataca ctgcgcggcg aactcgacac cctccgcacc cacagcagcg
     7381 gaacacaaag ccacatcctc ctccaacagc tccaactcat cgcccggcag ctcgaaccct
     7441 actaccgcgc ctaccgacaa attccgcaca ggcagcccca aaacgcagcc tgaaaaagtt
     7501 tcggcatttt gtaagagaga gcagattgtc agacaggttg caagatagtg gataaagctt
     7561 tgcgcagggt aaatgcgtag caactgaacc gtcattccca cgaacctaca tcccgtcatt
     7621 cccacgaaaa cagaaaacca aaaacagaaa cctaaaat
//
LOCUS       DAXHRI010000093         7556 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXHRI010000093 DAXHRI010000000
VERSION     DAXHRI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.093"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..419
                     /locus_tag="ACFM75_001437"
     CDS             <1..419
                     /locus_tag="ACFM75_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC6255969.1"
                     /translation="EAEWVGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKA
                     AAAGHGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHG
                     NLGWLLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="ACFM75_001438"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="ACFM75_001438"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC6255970.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            1376..1723
                     /locus_tag="ACFM75_001439"
     CDS             1376..1723
                     /locus_tag="ACFM75_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC6255971.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPYKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="ACFM75_001440"
     CDS             1728..2321
                     /locus_tag="ACFM75_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC6255972.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHIF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="ACFM75_001441"
     CDS             2385..2993
                     /locus_tag="ACFM75_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC6255973.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3654
                     /locus_tag="ACFM75_001442"
     CDS             3001..3654
                     /locus_tag="ACFM75_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6255974.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPAKPHKKEILDNLF"
     gene            3694..4473
                     /gene="map"
                     /locus_tag="ACFM75_001443"
     CDS             3694..4473
                     /gene="map"
                     /locus_tag="ACFM75_001443"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC6255975.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDNKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4628..4927
                     /locus_tag="ACFM75_001444"
     CDS             4628..4927
                     /locus_tag="ACFM75_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6255976.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5165..5539
                     /locus_tag="ACFM75_001445"
     CDS             5165..5539
                     /locus_tag="ACFM75_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6255977.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5727..7193)
                     /locus_tag="ACFM75_001446"
     CDS             complement(5727..7193)
                     /locus_tag="ACFM75_001446"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC6255978.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1949 a         2275 c         1850 g         1482 t
ORIGIN      
        1 aagaggcgga atgggtgggc agcctgttcg gcctgcacaa atctttcggt ttccttacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 atcgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcgccgcgcg attggttgcc
      661 gtcggcagac aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcattaatcg gcgttcgggg aacagttccg ccatatcggc
      781 aagcgtcgca ccgatgcggt gcggcgtttc aaacatcacg acgggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgctctcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat cccgccacac cggccacact caaagccgcc atcaccgcgc ttgcgccgac
      961 aacagggaca actttaaacc cgacctcacg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctgaaacata aaaaatgccg tctgaaaaac agacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg ctcgccttcc tccaatccca
     1441 aggctgcaca ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgccgca tacagcattt ccccatacaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggc tgacaaacgt accgtgccgc ctcgacgcgg tactcatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caaacaggaa gccgaaaaag
     1801 tactggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaatgacg
     1861 gcaaaatcct ggcctgcgga aacggcggtt cggccgccga tgcccagcac ttcgccgccg
     1921 aaatgaccgg tcgttttgaa aaagaacgca tggaactcgc cgccgtcgcg ctgacaacag
     1981 acacttccgc gctgaccgcc atcggcaacg actacggttt cgaccatata ttcagcaaac
     2041 aagtgcgcgc gctcggacgt gcaggcgacg tcttggtcgg catttccacc tccggcaatt
     2101 ccgccaacgt catcgaagcc gtcaaagccg cacacgaacg cgatatgcac gtcatcgccc
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatgctcaa agacaccgac gttctgctca
     2221 acgtccccta tccgcgcacc gcccgcattc aggaaaacca catcctgctg atacacgcca
     2281 tgtgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagca cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg aagcgccgcc gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgc tcctatctgc
     2581 gccaaaacaa ccaaaccaaa ggctacacgc cccaaatctc cgttgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgagaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtat acaactacat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgtcgtattg
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcggca aaaccctcca aaccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacac ccaaagaaaa agaaacgccc aaagaaaacc ataccaaacc ggacaccccg
     3601 aaaaacacgc cggccaaacc ccataaaaaa gagattctcg acaacctctt ctgacccggc
     3661 acggcaggca cacccgcaat ccaaggaagc attatgaacg gcatcatcat caaaaccccc
     3721 gaagaaattg agaaaatgcg cgaactgggc aaactcgtcg ccgaagccct cgactatatc
     3781 ggacaattcg tcaaacccgg cattaccacc gatgaaatcg acaaactcgt ttacgactac
     3841 cacgtcaacg tccaaggcgg ctaccccgcc cccctccact acggcaaccc gccctacccc
     3901 aaatcctgct gtacctccgt caaccacgtc atctgccacg gcattcccga caataagccg
     3961 ctcaaagaag gcgacattat caacatcgac ctcaccatca agaaagacgg cttccacggc
     4021 gactccagcc gtatgtttac cgtcggcaaa gtctccccca tcgcccaacg tctgatcgac
     4081 gtaacccacg cctctatgat ggcgggcata gaagccgtca aacccggcgc gacactgggc
     4141 gacgtaggtt acgcctgcca acaggttgcc gaaaacgccg gctattccgt cgtacaggaa
     4201 ttctgcggac acggcatcgg gcgcggtttc cacgaagccc cgcaagtgtt gcactacgga
     4261 aaaaaaggac agggccccgt tctaaaaccg ggtatgattt ttaccgtcga accgatgatc
     4321 aaccaaggca aacgccacct gcgtatcctc aacgacggct ggacggtggt taccaaagac
     4381 cgctccctct ccgcccaatg ggaacacgaa gtcttggtga ccgaaaccgg ctacgaaatc
     4441 ctcaccgtca gccccgcctc cggcaaaccc tgaaaccgga cgtatccgcc ccataaaaac
     4501 aaacaatgcc gtctgaaaga aacggcagat atgatatata atataaaaac aggcttgacc
     4561 cggcacatta cgaaaacaaa gcaaatcgga atttgccccg caaccagaca aacttaaagg
     4621 aagttttatg aaaatatttg aaaatataga agatgttaaa gccatccgta aaaagaccgg
     4681 gctgaaccag atagacttct ggggcaaggt cggcgttacc cagtctggag gctcgcgcta
     4741 cgaaaccggc cgcaaaatgc ccaaacccgt acgcgaactg ctccgcctcg tccatatcga
     4801 atgcatcgat ttggcgaaag tcaacaaaaa agatatggaa atcgccgccc tgttgaaaaa
     4861 acaccatccc gacctgtatg ccgaattgtc caaacagacc aagtccgaaa gaaaaaaaca
     4921 aagttaaacc gcaacctccg gatgcccgac agtttttcat ttccgaaaaa cgcaaacaat
     4981 gccgtctgaa acaccggaca ggccgccgta tcccgcctgc cgcccctgcc tcaaaccgcc
     5041 gaaccgcccg aacccgcctt tttacaaact ttatccaatt tcctgtttat ttcaggatac
     5101 gccgacatta gaatgtcaaa cagctcgaaa cgggcaaact ccacatccat ccaaaggaat
     5161 aaaaatgaaa cttctgacca ccgcaatcct gtcttccgca atcgcgctca gcagtatggc
     5221 tgctgctgcc ggcacgaaca accccaccgt tgccaaaaaa accgtcagct acgtctgcca
     5281 gcaaggtaaa aaagtcaaag taacctacgg cttcaacaaa cagggtctga ccacatacgc
     5341 ttccgccgtc atcaacggca aacgcgtgca aatgcctgtc aatttggata aatccgacaa
     5401 tgtggaaaca ttctacggca aagaaggcgg ttatgttttg ggtaccggcg tgatggatgg
     5461 caaatcttac cgcaaacagc ccattatgat taccgcacct gacaaccaaa tcgtcttcaa
     5521 agactgttcc ccacgttaat caggcaacaa aaaacagcgt tttcagaaat gaaaacgctg
     5581 tttttttgac cgttccatta ttcgcaaaag ggaaaaaacg attacctgcc ccgtgtatca
     5641 aaacctgccc tgccggatga agggcataac cggcagggac ggcgtcaaca ccatatgggg
     5701 gtacggcttt tcttgaaaga ttcggcttaa atatccaata ctttcgcggt ataggcgata
     5761 atttcatccg ccctttcagg gttttcgttc aacttgatgc cgtaacccgg taccagctct
     5821 ttcagacggt cttcccaaga cggggcgcgc tcggggaagc attggtgcat cagccggatc
     5881 atcagcggca cagcggtcga tgcgcccggc gacgcgccca gcaatgcggc gagtgagccg
     5941 tcggcgtggg cgacaatctc cgtaccaaac tggagcacgc cgcctttttc ggagtctttt
     6001 ttaatgattt ggacgcgttg ccctgcggtg atgagttccc agtcgtcggg gtttgcctcg
     6061 gggtagtatt ccagcaggga ggcgaagcgt tcttctttgg ttttacgcaa ttcgcccagc
     6121 aggtatttgg tcagcggcat attcgcccag ccggcgcaca gcataggata gaggttgtcc
     6181 atatggatgg acagcggcaa atccataagc gagccttgct tgaggaagtt ggaacggaag
     6241 cctgcgtaag ggccgaacat aaggtggcgt ttgccgtcca cgttgcgtgt gtcgaggtgc
     6301 gggacggaca tcggcggcgc gccgacggaa gcctgcccgt acactttggc gttgtgttgt
     6361 tcggcggttt cggggttgct gttgcggaag aacaggccgg acacggggaa gccgccgtag
     6421 cctttgcctt cggggatgcc ggatttttgc agcagggtca gcgcgccgcc gcccgcgccg
     6481 aggaagagga agcgggtacg gagggtgagc tgcccgtcgg ggttgcgggt atcggcggtt
     6541 ttgagcaccc acgcgccgtc ggattcgcgt ttgatgtctt cgacgtggcg gttgaactcg
     6601 gtttttacgc ccttgccctg caaatatttc accatttggc gcgtcagccg tccgaaatcg
     6661 acatccgtac cttcggcgga gtagttggcg gcgacgggtt ggttttcgtc ccggccgcgc
     6721 atcatcagcg gagcccaatc ggaaattttg ttccgatcgg tggaaaattc catattttca
     6781 aaaagttttt gggttttaaa cgcgtcataa cgtttttgaa gataagaaca atggtcttca
     6841 ttcatcacca aagacatatg cggcacggca ttgatgaagg aattgtcttc caacttgcct
     6901 tccgcgacca gcgtcgccca aaactggcgg ctgacatgaa actgttcggc aatattgagg
     6961 gcgcgcgccg gatcgataat cccatttgca cccaacggcg catagttcaa ttcgcacagc
     7021 gcggaatgcc ccgtgccggc gttgttccac gcgtttgacg attccaacgc cacatcttcc
     7081 aagcgttcaa tcagggtgat ttcccaagac ggttcgagtt ctttgagcaa aacgcccaaa
     7141 gtcgcgctca taatgccgcc gcccaccaag acaacgtctg tcgcttcagc catggtttac
     7201 tcctaaaaaa caggcatctt ctgcccttat ggttatttgc cgtactacaa acgcctgaat
     7261 cgcaaaagca gggaaaaccg gcaatggtgt gtgtccgagt atgctgtttc ggggttggaa
     7321 tgcgttgcaa gcatggcttc cgacaccgct tcaggggctt gtaatatgtt atcgtgaatg
     7381 tagtggattt tactgggaaa tgcaaagttt ttctgtcgcc cgccaagtcg ggaaactgcg
     7441 aaatgaaaaa taaaaatagt tatttatcta tatatatcaa atttttaata gataaaaaat
     7501 caaaattgtt tatatattaa tttttaaaag attgtcagca tattgcgtta agtttt
//
LOCUS       DAXHRI010000094         7509 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXHRI010000094 DAXHRI010000000
VERSION     DAXHRI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.094"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(427..1467)
                     /gene="queA"
                     /locus_tag="ACFM75_001447"
     CDS             complement(427..1467)
                     /gene="queA"
                     /locus_tag="ACFM75_001447"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC6255979.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LQDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            1584..1841
                     /locus_tag="ACFM75_001448"
     CDS             1584..1841
                     /locus_tag="ACFM75_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC6255980.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            1823..2278
                     /gene="accB"
                     /locus_tag="ACFM75_001449"
     CDS             1823..2278
                     /gene="accB"
                     /locus_tag="ACFM75_001449"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC6255981.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRIIRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2390..3751
                     /gene="accC"
                     /locus_tag="ACFM75_001450"
     CDS             2390..3751
                     /gene="accC"
                     /locus_tag="ACFM75_001450"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC6255982.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            3869..4756
                     /gene="prmA"
                     /locus_tag="ACFM75_001451"
     CDS             3869..4756
                     /gene="prmA"
                     /locus_tag="ACFM75_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC6255983.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            4774..5337
                     /gene="orn"
                     /locus_tag="ACFM75_001452"
     CDS             4774..5337
                     /gene="orn"
                     /locus_tag="ACFM75_001452"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC6255984.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPSVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            5348..5620
                     /locus_tag="ACFM75_001453"
     CDS             5348..5620
                     /locus_tag="ACFM75_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HFC6255985.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(5836..7119)
                     /gene="hemL"
                     /locus_tag="ACFM75_001454"
     CDS             complement(5836..7119)
                     /gene="hemL"
                     /locus_tag="ACFM75_001454"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC6255986.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFAADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(7183..>7509)
                     /locus_tag="ACFM75_001455"
     CDS             complement(7183..>7509)
                     /locus_tag="ACFM75_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6255987.1"
                     /translation="TEEGIAQTVVKGKTTRAEVEARFGKRNPFGCYAYHEVSLPIYNF
                     LPTNFIYMKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVIRKEAGKS
                     LSQPEK"
BASE COUNT         1835 a         2225 c         1885 g         1564 t
ORIGIN      
        1 caagtggaaa aatacttggc agattatcag gcacacttgg aaaaagtgtt cggctaaaaa
       61 tttatcttat aaacaaacaa aggcagcctg aaagattgaa tggtctgcac ccctaaggtt
      121 ggactaacca accgactaag gtgcagatta ttttttgttg ctttttcagc ttttcgttgg
      181 gttagatatt cttgcccact gttttcaggc agccttgaat acaaaaaaat ggcgtatgta
      241 atatgtttat acgaccaaaa cggaatgaat tttaacgtat tgcgcgtcat caacaatgac
      301 tgagtttctc gcctctcgcg cctgaatcta tgtcatacag ttcctttcat tcaaatcaac
      361 aaaagaatgc cgtccgaacg tccgttcaga cggcacttgt cttcccccaa tagacttggg
      421 gctgttctaa cgtaccaccc cttcgttccg ccccaaaacc atcgcatcgc cgtagctgaa
      481 gaaacggtat tcgcgttcga ccgcataacg atacgcggcg cggatatgac ccatacccga
      541 aaacgcgctg acaagcatca gcagcgtcga tttcggcaaa tgaaaattgg taaccagcct
      601 gtcgacaaca ttaaaacggt agcccggcgt gatgaaaata tcggtgtcgc cctgccccgc
      661 tttcagacga cccgtcgcac gcgcggcaga ttcgagggcg cgcatggaag tcgtgccgac
      721 cgcccagact ttgttccccc gggcttttgc cgcctcaacg gcggcggcgg tttcagacgg
      781 cacttcaaac cattcgctgt gcattttatg ctcttcgatt ttgtccacac gcacgggttg
      841 gaatgtcccc gcaccgacgt gcagagttac ttctgcggtt accgcgcctt tgtctttcag
      901 gcggcgcaaa agttcttccg taaaatgcag acccgccgtc ggcgcggcga ccgcgccctg
      961 atatttggca taaacggttt gataacggct gtcgtcatcc gcatcggcgg cgcgttcgat
     1021 ataaggcggc aggggcaggt gtccgttctg ttccaaaagt tcgtaaacgg tttgaccgcc
     1081 ttcaaaacgc aggcagaaca gttcgccctc acgcccgacc gtcacggcgc ggatgccgcc
     1141 ttcaaacacc agccccatac cgggcttggg cgatttggac gaacggatgt gcgccagtgc
     1201 ggtatggttg tccaacacgc gctcaatcag ggcttcgatc ctgccgccgc tgtctttctg
     1261 cccaaacagc cgcgccttca tgactttggt gttgttgaac accaaaacgt cgcccgcctc
     1321 aacataatcc tgcaaatcgc cgaacacccg gtcttgcagc ggcatatcgg gcaacgcaac
     1381 caaaaggcgg ctgctgccgc gcacttcggg cggatgctgg gcaatcagct tttcgggcag
     1441 ggtaaaatca aaatctgaaa tatccatttt tacactctcg ttcgggcaag ccgccattat
     1501 acgcacttta gccctttttc agacggcatc tttgtccgaa aaaccaacag attagaataa
     1561 acactcttaa cctggaacat cttgtgcgca aaatcaaact tcctgcacat ttcccccaaa
     1621 aaccgccgtt ttttgatatt ttactggaca tttaccgaca acttcgggaa aataaacaca
     1681 ttctcacggt tgttttccac cacaggaaaa ccgtatccga acaccattcc gcccggtttg
     1741 cgccgttgcc gcaagccggc tgttttctga aaaaccaacg caacaacccg ccggaacacc
     1801 ggcagccttt aaaggaacag aaatggattt gcgcaaatta aaaaaactga ttgatttggt
     1861 tgaagaatcg ggtatcgccg aaatcgaagt aaccgaaggc gaggaaaaag tccgcatcat
     1921 ccgaaccatc gccgccgcac ccgtttacgc cgcgcccgta cctgccgccg cgccggccgt
     1981 aacgcctgcc gccgcacccg ttgcggcatc cgcgcccgcc gccgcacctg ccgcccgcga
     2041 tttgtccgac gcgcaaaaat cgcctatggt cggcacgttc taccgcgcac ccggcccgaa
     2101 tgccgcgcct tttgtcgaag tcggccaaca ggttaaagcc ggcgacacgc tgtgcatcat
     2161 cgaagcgatg aagctgatga acgaaatcga agccgaaaaa tccggcacgg tcaaagaaat
     2221 tttggtcgaa aacggtacgc ccgtcgaatt cggcgaaccg ctcttcatta tcggataatc
     2281 ctgttttcag acggcataaa cttccgatgc cgtctgaaat gctttccccc ttcagcgttc
     2341 ccgcaccctt ttttacggac gggttgccgg aaccgcagga aaggtcatca tgctgaaaaa
     2401 agttttaatc gccaaccggg gcgaaatcgc attacgcgta ctccgtgcct gccgcgaaat
     2461 gggcattgcc accgtcgccg tgcattccga ggccgacaaa gacagcctgc acgtcaaact
     2521 cgccgacgaa tccgtgtgca tcggccctgc cgcttccgcg caaagctacc ttaacgtccc
     2581 cgccattatc gccgccgccg aagtaagctg cgcggacgct gtccatccgg gctacggttt
     2641 ccttgccgaa aacgccgatt tcgccgaaca ggtcgagcag tccggcttta cctttatcgg
     2701 cccgaaaccc gacaccatcc gcctgatggg cgacaaagtc tccgccaaac acgcgatgat
     2761 agcggcaggc gtaccctgcg tccccggttc tgacggcgca ttgcccgacg acggcgaaga
     2821 aatcctcaaa atcgccgata aagtcggtta tcccgtcatt atcaaagcct ctggcggcgg
     2881 cggcggtcgc ggtatgcgcg tggtcgagaa aaaagaagac ctcctccaat ctgtcgaaat
     2941 gaccaaggca gaagcaggcg cggcattcgg caacccgatg gtttacatgg aacgctatct
     3001 gcaacgtccg cgccacgtcg aaatccaagt gattgccgac gaacacggca acgccatcta
     3061 ccttgccgag cgcgactgtt cgctgcaacg ccgccaccaa aaagtcatcg aggaagcacc
     3121 ggctccgttc atcactgaaa aagaacgcgc caaaatcggc aacgcctgtg ccgacgcctg
     3181 caaacgcatc ggctatcgcg gcgcaggtac gtttgaattc ctgtacgaag atggcgaatt
     3241 tttctttatc gagatgaata cgcgcgttca ggttgagcat ccggttaccg agctgattac
     3301 cggcgtggac atcgtgcagg agcaactccg catcgccgcc ggcctgcctt tgcaatacaa
     3361 acaaaaggat attcaagtcg aaggccacgc gtttgagtgc cgcatcaatg ccgaagaccc
     3421 gtacaacttc atccccagcc cgggcccgat tgaaagctgc cacctgcccg gcggcttcgg
     3481 tatccgcgtg gacagccaca tttaccaagg ctaccgcatc ccaccgtact acgacagcct
     3541 gatcggcaaa atctgcgtac acggcaaaac gcgtgaacag gcaatggcga aaatgcgcgt
     3601 cgcactcgcc gagctggcgg taaccggcat caaaaccaat acgccgcttc accgcgacct
     3661 gttcgccgat gcgggtttcc aaaaaggcgg cgtcagcatc cactatttgg aacactggct
     3721 ggaagatcgc aaagccaaac aggacaaata agccgtcccc gatatgccgt ctgaaacagt
     3781 ttgtccgttt tcagacggca tttccgttac acacccatcc ccttatacag tacgacccta
     3841 ttccgaaggt ctgttaagaa agcccgctat gccctaccaa caaatcaccg tcaacgtcaa
     3901 cgatgccgtc gccgaacgcc tcgccgacgc gctgatggaa cacggcgcac tctccgccgc
     3961 catcgaagat gcctacgccg gcacgcaaaa cgaacaggcg attttcggcg aacccggtat
     4021 gcccgccgaa caaatctggc agcagagcaa agtcatcgcc ctgttcggcg aacacgacga
     4081 agccgccgcc atcatccaaa tcgccgcaca agaatgcggg ttaaaagact tggcatacac
     4141 cggcgaaacc atcgaagacc aagactgggt gcgtctcacg caatcgcaat tcgaccccat
     4201 ccgaatttcc gaccgcctgt ggattacccc ctcttggcac gaagcccccg aaggcactgc
     4261 cgtcaaccta cgcctcgatc ccggcctcgc cttcggcact ggcagccacc caaccacgcg
     4321 cctctgcctc aaatggctgg atacgcaact caaaaacggc gaaagcgtcc tcgactacgg
     4381 ctgcggttcg ggcatcctga ccatcgctgc cctcaaactc ggcgcaggtt ctgccgtcgg
     4441 cgtcgatatt gacgaacaag ccgtccgcgc cggcaaggac aacgccgaac aaaacaacgt
     4501 cgatgcacaa ttcttcctgc ccgacggcct gcctcaaggg caattcgacg tagttgtcgc
     4561 caacatcctc gccaaccctt tacgtatgct cggcgaaatg ctcgccgccc gcaccaaaca
     4621 aggcggccgc atcgtgttat ccggtttgtt ggacgaacaa gccgaagaac tcggcggcat
     4681 ttacagccaa tggttcgatc tcgatcctgc ggaaacagaa gaaggatggg cgcgactgag
     4741 cgggacaaaa cgctgacaag caaaggaaac atcatgcagg ataaaaacaa cctatgctgg
     4801 ctcgatatgg aaatgacggg gcttaatccc gaaaccgacc gcattatcga agtcgcgatg
     4861 attattaccg actcggattt gaatgtattg gcgcaatccg aagtttacgc catccaccaa
     4921 agtgacgagc tgctcgacaa tatggacgag tggaacaccg ccacacacgg caggacgggg
     4981 ctgacgcagc gcgtacgcga atcgtcgcat accgaagccg aagtcgaaca gaaactgctg
     5041 gactttatgt cggaatgggt acccggacgc gccacgccga tgtgcggcaa ctccatccac
     5101 caagaccggc gttttatggt caaatatatg ccgaaactgg aaaactactt ccactaccgc
     5161 aacctcgacg tttccacgct caaagagctt gccaaacgct ggaatccgtc cgttgccaaa
     5221 agcgtcgtca aacgcggttc gcacaaggca ttggacgaca ttttggagag catcgaagaa
     5281 atgcgccact accgcgaaca ctttctgatt tccgccccga gagccgaagc gcaataagaa
     5341 acaaaaaatg ccgtctgaag cgcaaatcct gcatttcaga cggcattttt acagcagatt
     5401 gaaatcaaaa atatacacgc ccgtcattcc cgcgcaggcg ggaatccgga aggtcgggct
     5461 tgttggtcat gaagtggtct tgaaaatgaa acgttatcga aaatctgacg aactgaaaga
     5521 aaaatatagc gatatagatg agacttttga atttataaag gggtttatct atgtaattgc
     5581 ccttgctttt accgcatatg tcgctatccg ttttttctga tgcgcttccg ataaaatgcc
     5641 gtctgaaggc ttcagacggc attgtttcag gtggcggcgg cttcctgccg ggcgcggctg
     5701 atttcggccc gttattttca atcattacag aaactgaaag gtctggattc ccgcctgcgc
     5761 gggaatgacg gcggtgtgca ttcttacttc aatctgccaa acaaatcgaa cagagaaact
     5821 ctgtccgtca aaacatcatg cagccatcgc cttgaacact tcaaccgcaa ccgcaaccgt
     5881 ttcgtcaatc agctcgggcg tatgcgcggc agaaacgaaa cctgcttcat aagcggacgg
     5941 gccgaaggca atgccgcggt cgagcatacc gtggaagaag cgtttgaaag cgtcaatatt
     6001 ggaacgcgcc atatcggcat agtttcgcgg cgcgtgtgcg gcgaaataca gaccgaacat
     6061 accgcccacg ctgtcggcgg caaactcgat gcccgccgca tccgctgccg tctgaaaacc
     6121 ttgaaccaac tgttcggtac gcgccgtcag gttttcataa aacccttcgc ggcggatgat
     6181 ttccagcgtt ttcaaaccgg cggcgacggc aatcgggttg cctgataatg tacctgcctg
     6241 atacacgccg cccagtgggg aaatacattc cataatgtct ttgcgcccgc cgaacgcggc
     6301 aagcggcata ccgccgccga tgactttgcc catcgtggtc aggtcgggca tgatgccgtg
     6361 cagcgattgt gcgccgccga gcgcgacgcg gaaaccggtc atcacttcgt cgtaaatcaa
     6421 caccgcgccg tgtttttcgg tcaattcgcg cagggctttg acgaaggctt cggtcgggcg
     6481 gacaaggttc atattgccga caaaaggctc caaaatcaca caggcaatgt cattgccgct
     6541 ttgagcaaag gcttcttcca attgggcgat gttgttgtac tcaaggacta aagtgtgttt
     6601 ggtaaagtcg gcaggcacac cggcggaaga gggattaccg aacgtcagca ggccgctgcc
     6661 ggctttcacc agcaggctgt cggaatgccc gtggtagcag ccttcaaact tgatgatttt
     6721 gtcgcgaccg gtaaaaccgc gtgccaaacg gatggcggtc atcgtcgctt cggtaccgga
     6781 gctgacgagg cgcagccgtt cgacggacgg catgattttg gcgatttgtt cggcaatggc
     6841 gatttcgccc tcggtgggcg cgccgaacga caaaccgccc aacgcggctt cgcgcacggc
     6901 ttcgacgact tcggggtgcg cgtgtccgac aatcgcaggc ccccaagagc cgacatagtc
     6961 ggtataacgc gtgccgtttt cgtcccaaac atacgcgcct tcggcttttt tgatgaagcg
     7021 cggcacgccg ccgacgctgc cgaatgcgcg gacgggggaa ttcacgccgc cggggatgat
     7081 ggctttggcg cggtcgaata aaatttcgtt acggttcata tttatcctca aatgccgtct
     7141 gaacggcagg tttcgggctt agaagcagaa agtcccattt tatcattttt caggttgcga
     7201 caaggatttg cctgcttctt tgcggatgac gccaaccgca tcccggatga cggaacgctc
     7261 gtctttttcc actttatgtg taaagcggta gtcccggaca actccttccc agtcgtaatc
     7321 cacacaccac tcccaatgcc ggcgttctga tttcatataa atgaaattgg tcggcaaaaa
     7381 attataaatc ggcaggctga cttcatgata ggcataacaa ccgaaagggt tgcgcttccc
     7441 gaaacgtgcc tctacctccg cccgagtcgt tttgccttta acaaccgttt gtgcgattcc
     7501 ctcttccgt
//
LOCUS       DAXHRI010000095         7498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXHRI010000095 DAXHRI010000000
VERSION     DAXHRI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.095"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            26..2041
                     /locus_tag="ACFM75_001456"
     CDS             26..2041
                     /locus_tag="ACFM75_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC6255988.1"
                     /translation="MKSYPDPYRHFEDLDSAETQNFAAEANAETRTRFLNNDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPGWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWGERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGASWTDEYGNPQKYEVCKCRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(2155..3111)
                     /locus_tag="ACFM75_001457"
     CDS             complement(2155..3111)
                     /locus_tag="ACFM75_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6255989.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRSFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            3347..4312
                     /locus_tag="ACFM75_001458"
     CDS             3347..4312
                     /locus_tag="ACFM75_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6255990.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
     gene            4857..5180
                     /locus_tag="ACFM75_001459"
     CDS             4857..5180
                     /locus_tag="ACFM75_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6255991.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            5294..7471
                     /gene="tdfF"
                     /locus_tag="ACFM75_001460"
     CDS             5294..7471
                     /gene="tdfF"
                     /locus_tag="ACFM75_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC6255992.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRIMPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPATKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         1688 a         2276 c         2143 g         1391 t
ORIGIN      
        1 acctgcccaa ccctcaagga ccccgatgaa atcctacccc gacccctacc gccattttga
       61 agacctcgat tccgccgaaa cgcaaaactt cgctgctgaa gcgaatgccg aaacgcgcac
      121 gcgtttttta aacaacgaca aggcacgcgc attgtctgac ggcattttgg cgcagttgca
      181 ggacacgcgg caaattccgt tttgtcagga acaccgtgcg cggatgtacc atttccatca
      241 ggacgcggaa tatccgaagg gcgtgtaccg cgtgtgtacc gcggcgacat accgttccgg
      301 ctatcccggg tggaaaatcc tgttttcagt ggcggatttc gacgaattgc tcggcgacga
      361 tgtatatttg ggcggcgtgt cgcacttggt ggaacagccc aaccgtgcgt tgttaacact
      421 gagcaaatcg ggcggcgata cggcgtacac gctggaagtg gatttggaag caggggagtt
      481 ggtagaaggc ggttttcact ttccggcagg caaaaaccat gtgtcgtggc gcgatgaaaa
      541 cagcgtgtgg gtgtgtccgg cttggggcga acgccagttg accgaatcgg gctatccgcg
      601 cgaggtgtgg ctggtggaac gcggcaagag tttcgaggaa agcctgcctg tgtatcaaat
      661 cgataaaggt gcaatgatgg tgaacgcgtg gcgttatctc gatccgcaag gttcgccgat
      721 tgatttgatt gaagcgtcgg acggttttta caccaaaacc tatttgcagg tgtcgtcgga
      781 aggcgaggcg aaaccgttaa acctgcccaa cgattgcgac gtggccggct atctagccgg
      841 acatcttttg ctgacattgc gtaaagactg gcaccgcgcg aaccaaagct atccgagcgg
      901 cgcattggtg gcagtaaaat taaaccgagg cgaactcggg gcggcgcagc ttttgtttgc
      961 gcccgatgaa acgcaggcat tggaaagcgt ggaaacgacc aagcgttttg tggtggcgag
     1021 cctgctggaa aacgtacaag gccgtctgaa agcgtggcgg tttgccgacg gcaaatggca
     1081 ggaagtcgaa ctaccgcgcc tgccttcggg cgcgttggaa atgaccgacc agccatgggg
     1141 cggcgatgtg gtttaccttg ccgccagtga tttcactacg ccgctgacgc tgtttgcgct
     1201 ggatttgaac gtgatggaac tgaccgtcat gcgccgtcag ccgcagcagt tcgattcaga
     1261 cggcatcaac gtgcagcagt tttggacgac ttcggccgac ggcgagcgta ttccttattt
     1321 tcacgtcggc aaaaacgccg cccccgacac gccgactttg gtttatgcat acggcggttt
     1381 tggcattccc gagttgccgc attatttggg cagcgtcggc aaatattggc tggaagaggg
     1441 caatgccttt gtgttggcaa acatccgcgg cggcggcgag ttcggcccgc gctggcatca
     1501 ggcggcgcag ggaatcagca aacacaaaag cgtcgacgat ttgcttgccg tcgtgcgcga
     1561 tttgtccgaa cgcggcatca gttcgcccca acacatcggc ttgcagggcg gcagcaacgg
     1621 cggcctgatt accgccgccg ccttcgtgcg cgaaccgcaa agcatcggcg cgctggtgtg
     1681 cgaagtgccg ctgaccgata tgatccgcta tccgctgctc tccgccggcg caagctggac
     1741 ggacgaatac ggcaacccgc agaaatacga agtctgcaaa tgccggttgg gcgaattgtc
     1801 gccgtatcac aatctttcag acggcatcga ttatccgcca gcgctcatta ccaccagcct
     1861 cagcgacgac cgcgtccatc ctgcccacgc gctcaagttc tacgccaaac tgcgcgaaac
     1921 ctccgcgcaa tcttggctct actcacctga cggcggcggc cataccggca acggcaccca
     1981 acgcgaatcc gccgacgaac tcgcctgcgt gttgctgttt ttgaaagagt ttttgggcta
     2041 agggcgggga gcggcactgc cgccgcgaat gaaaaaaggt cgtctgaaac tgctttttca
     2101 gacgaccttt tttaatggtt gtttcaaatc aaaatatcta tgccgccggc cccatcagca
     2161 cttcttcaca tccgaaggca aaaatccgta atgccgtctg aacgcttcgt tgaaccgtcc
     2221 cgcgtggcgg tagccgcaaa agtgcatggc ggcttggacg gtgctgccgg attcgatgag
     2281 ggcgagcgcg tgttccagcc gcaggcggcg caggcatccg gcgacggttt cgccggtttg
     2341 cgctttgaaa tagcgtttca ggtagcattc gttcagtccg acgcggcggg cgatttcggc
     2401 gatggtcagc ggacgggcga attcgtgttg caggatgtcg gcggcttcgt ctatgcgccg
     2461 acggcggtaa ccgttgtcgt ggcggcggaa ggtgaagcgc aataatcggg cggagagttc
     2521 cagcgcggcg gcttcgtcgg caagcaggcc gaagccgtcc gattcgaacg agcgttgcag
     2581 cagtgggcag gccgccgccg tcagtgccgc tgcgttttgc gccagccgtt gcagggcgaa
     2641 tcggcctatc gtttgcggcg aaaacaggcg ttcgtccagc aagccttcgt cgtgccagcg
     2701 gcgcagtttt tccagcgaaa aatccaaatg cagcgcgcac atgccgctgt tgtcgggcag
     2761 cagggtttcg gatacgtccg ccaactcgcc gcgtaccagc cagatttcgc cggcagacgg
     2821 gcggtattcc ctgccgccca tttgtaaccg gttctgcccc gacaccatga cgaacaaggc
     2881 gcagttgtgg ctgaaattgt ggatttcggt gggaaacgcg cccgttccgc cgccgcgcat
     2941 ccgcgacaag gtgatgcccg agtcgaagcg gttgatgcac atttccagat gcaaaccggg
     3001 ctgttttgcc tgcgcaatga gcgcgctgtc ggaacagccg tccaacgccc agccggattt
     3061 atcggagcgg acataggttt ggtactggcg gtagatggcg gcggtgttca tgattggata
     3121 ggaacgagtt gtctaacaaa tgaattaaat aggaattatt accaataatc aagcgcaggg
     3181 attggttgaa acggaaaagg tcgtctgaaa gggtgtttca gacgaccttt tccgtatcgg
     3241 gaatttgttt tgccgtatcg ggaattttgc gttttgcggc gtggtttctg caggttgttt
     3301 gcttaataat aaacattctt atccgtatgc aaaggaaccg cacaccgtga aaccgcgttt
     3361 ttattgggca gcctgcgccg tcctgctgac cgcctgttcg cccgaacctg ccgccgaaaa
     3421 aactgtatcc gccgcatccg catctgccgc cacactgacc gtgccgaccg cgcggggcga
     3481 tgccgttgtg ccgaagaatc ccgaacgcgt cgccgtgtac gactgggcgg cgttggatac
     3541 gctgaccgaa ttgggcgtga atgtgggcgc aaccaccgcg ccggtgcgcg tggattattt
     3601 gcagcctgca tttgacaagg cggcaacggt ggggacgctg ttcgagcccg attacgaagc
     3661 cctgcaccgc tacaatcctc agcttgtcat taccggcggg ccgggcgcgg aagcatatga
     3721 acagttggcg aaaaacgcga ccaccataga tctgacggtg gacaacggca atatccgcac
     3781 cagcggcgaa aagcagatgg agaccttggc gcggattttc ggcaaggaag cgcgcgcggc
     3841 ggaattgaag gcgcagattg acgcgctgtt cgcccaaacg cgcgaagccg ccaaaggcaa
     3901 aggacgcggg ctggtgctgt cggttacggg taacaaggtg tccgccttcg gcacgcagtc
     3961 gcggttggca agttggatac acggcgacat cggcctaccg cctgtagacg aatctttacg
     4021 caacgagggg cacgggcagc ctgtttcctt cgaatacatc aaagagaaaa accccgattg
     4081 gattttcatc atcgaccgta ccgccgccat cgggcaggaa gggccggcgg ctgtcgaagt
     4141 attggataac gcgctggtac gcggcacgaa cgcttggaag cgcaagcaaa tcatcgtcat
     4201 gcctgccgcg aactacattg tcgcgggcgg ctcgcggcag ttgattcagg cggcggagca
     4261 gttgaaggag gcgtttgaaa aggcagaacc cgttgcggcg gggaaagagt agggagtcgt
     4321 ctgaaaacgg agtttccgaa ggaagcgggg ggtttctgcg aagctaaagt gcggtttcaa
     4381 cgaattgaaa agcagcctgt atgttgaaaa taccgctcaa gcaaacctac ggtttgccgc
     4441 cctctcccta gccctctccc acagggagag gggattgggg tgcaggctgc ctttaaggtt
     4501 taggcaaatt tttaacttcg ttgaagctgc gatttcagaa gctccgtttt agcttcgcag
     4561 aaactccgct tccttcgaaa gctccgtttt cagacgacct tttggagtac cgcaggcaca
     4621 cgcatcgaac ggctgaatca aagattcaga ccgatggcag tccgcacccg agtttatgcg
     4681 gcaaacagcg aggctacggc aacccgtccc ctctccctgt gggagagggt tagggagagg
     4741 gcggtaagcc gcaggcttgc atcaaagtcg ataacgcttt cgttacaact ccgcccactg
     4801 aacgaagcca aaacgacaaa ccgcatcgta aaccacccaa cccataggag aaccccatgc
     4861 aaaacgaaac catcaacctg aaacagcacc ttgccgccat caaagaatac tggcagcccg
     4921 aaatcatcaa ccgccacggg ttccaattcc gtttggtcaa acttttgggc gattacggct
     4981 ggcatacgca cggatacagc gacaaagtgc tgtttgccgt ggagggcgac atggcggtgg
     5041 acttcgccga cggcggcagc atgacgatac gcgagggcga gatggcggtc gtgccgaagt
     5101 cggtgtcgca ccgcccgcgt tcggaaaacg gctgctcgtt ggtgctgatt gagttgtccg
     5161 acccgtccga ggccgtctga aaacgaagtt tccgaaggaa gctgagtttc tgcgaagcta
     5221 aaagcagcct gcaccttcaa tcaatatgcc gaaaatacaa ccccaccgca caccaacaca
     5281 caaaggaaat cccatgacac gcttcaaata ttccctgctg tttgccgccc tgttgcccgt
     5341 gtacgcgcag gccgatgttt ctgtttcaga cgaccccaaa ccgcaggaaa gcactgaatt
     5401 gccgaccatc accgttaccg ccgaccgcac cgcgagttcc aacgacggct acactgtttc
     5461 cggcacgcac accccgctcg ggctgcccat gaccctgcgc gaaatcccgc agagcgtcag
     5521 cgtcatcaca tcgcaacaaa tgcgcgacca aaacatcaaa acgctcgacc gcgccctgtt
     5581 gcaggcgacc ggcaccagcc gccagattta cggctccgac cgcgcgggct acaactacct
     5641 gttcgcgcgc ggcagccgca tcgccaacta ccaaatcaac ggcatccccg ttgccgacgc
     5701 gctggccgat acgggcaatg ccaacaccgc cgcctatgag cgcgtagaag tcgtgcgcgg
     5761 cgtggcgggg ctgctggacg gcacgggcga gccttccgcc accgtcaatc tggtgcgcaa
     5821 acgaccgacc cgcaagccat tgtttgaagt ccgcgccgaa gcgggcaacc gcaaacattt
     5881 cgggctgggc gcggacgtat cgggcagcct gaacaccgaa ggcacgctgc gcggccgcct
     5941 ggtttccacc ttcggacgcg gcgactcgtg gcggcagcgc gaacgcagcc gcgatgccga
     6001 actctacggc attttggaat acgacatcgc accgcaaacc cgcgtccacg caggcatgga
     6061 ctaccagcag gcgaaagaaa ccgccgacgc gccgctcagc tacgccgtgt acgacagcca
     6121 aggttatgcc accgccttcg gcccgaaaga caaccccgcc acaaattggg cgaacagccg
     6181 ccaccgtgcg ctcaacctgt tcgccggcat cgaacaccgc ttcaaccaag actggaaact
     6241 caaagccgaa tacgactaca cccgcagccg cttccgccag ccctacggcg tagcaggcgt
     6301 gctttccatc gaccacaaca ccgccgccac cgacctgatt cccggttatt ggcacgccga
     6361 cccgcgcacc cacagcgcca gcgtgtcatt gatcggcaaa taccgcctgt tcggccgcga
     6421 acacgattta atcgcgggta tcaacggtta caaatacgcc agcaacaaat acggcgaacg
     6481 cagcatcatc cccaacgcca ttcccaacgc ctacgaattt tcccgcacgg gcgcctatcc
     6541 gcagccatca tcgtttgccc aaaccatccc gcaatacggc accaggcggc aaatcggcgg
     6601 ctatctcgcc acccgtttcc gcgccgccga caacctttcg ttgatactcg gcggcagata
     6661 cagccgttac cgcaccggca gctacgacag ccgcacacaa ggcatgacct atgtgtccgc
     6721 caaccgtttc accccctaca caggcatcgt gttcgacctg accggcaacc tgtcgcttta
     6781 cggctcgtac agcagcctgt tcgtcccgca atcgcaaaaa gacgaacacg gcagctacct
     6841 gaaacccgta accggcaaca atctggaagc cggcatcaaa ggcgaatggc ttgaaggccg
     6901 tctgaacgca tccgccgccg tgtaccgcgc ccgtaaaaat aacctcgcca ccgcagcagg
     6961 acgcgacccg agcggcaaca cctactaccg cgccgccaac caagccaaaa cccacggctg
     7021 ggaaatcgaa gtcggcggcc gcatcatgcc cgaatggcag atacaggcag gctacagcca
     7081 aagcaaaacc cgcgaccaag acggcagccg cctgaacccc gacagcgtac ccgaacgcag
     7141 cttcaaactc ttcaccgcct accactttgc ccccgaagcc cccagcggct ggaccatcgg
     7201 cgcaggcgtg cgctggcaga gcgaaaccca caccgaccct gccacgctcc gcatccccaa
     7261 ccccgccacc aaagcccgcg ccgccgacaa cagccgccaa aaagcctacg ccgtcgccga
     7321 catcatggcg cgttaccgct tcaatccgcg cgccgaactg tcgctgaacg tggacaacct
     7381 gttcaacaaa cactaccgca cccagcccga ccgccacagc tacggcgcac tgcggacagt
     7441 gaacgcggcg tttacctatc ggtttaaata aggtcgtctg aaaacagagt ttctgcga
//
LOCUS       DAXHRI010000096         7404 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXHRI010000096 DAXHRI010000000
VERSION     DAXHRI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.096"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(56..2092)
                     /locus_tag="ACFM75_001461"
     CDS             complement(56..2092)
                     /locus_tag="ACFM75_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC6255993.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAYEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(2180..3052)
                     /locus_tag="ACFM75_001462"
     CDS             complement(2180..3052)
                     /locus_tag="ACFM75_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC6255994.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3322..5712
                     /gene="gyrB"
                     /locus_tag="ACFM75_001463"
     CDS             3322..5712
                     /gene="gyrB"
                     /locus_tag="ACFM75_001463"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC6255995.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASAETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLHAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            5811..7196
                     /locus_tag="ACFM75_001464"
     CDS             5811..7196
                     /locus_tag="ACFM75_001464"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC6255996.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
BASE COUNT         1662 a         2074 c         2112 g         1556 t
ORIGIN      
        1 gacggtgcgg atgtttttct atcgaatcct cctatttttt acttcaccct tgccgtcagg
       61 ccgcgttgtc gaagccgctg tggcgcaaga gtgcgtctat gcgcggttcg cgtccgcgga
      121 aggctttgaa ggattctgcc gcgctgcgcg atccgccgac ggcgaggatt tcctgccaaa
      181 agcgtttgcc tgtggcggcg acatcgtcgc tttcttcaaa ggcggcgtat gcgtccgcgc
      241 tcaatacttc cgcccacgcg tagctgtaat agcctgcgga atagccgcct gcgaagatgt
      301 ggccgaagct caaggcgaag cggttgtatt cgggcggttg gatgacggcg acttctttgc
      361 gcacgctgtc taaaacctgt tgccagtttt tcagacggcc ttcgtcgtct tcgctgtaaa
      421 tcatcatatc aaagagggcg aactccattt gccggacgag gaacatgccg cgttggaagt
      481 ttttggcggc gagcattttg tcgaagagtt ctttcggcag gggaacgccg gtttcttcgt
      541 aggcggacat ttgtgccaag acgtcgtatt cccagacgaa attttccata aactggctgg
      601 gcagttcgac tgcgtcccat tctacgccgt tgatgccgga tacgcccagt tcgtccactt
      661 gggtaagcag gtggtgcagc ccgtgtccgg tttcgtggaa gaggatgagg atttcgtcgt
      721 ggctcaaacg cgcttctctg ccgccgacgg gcggggcgaa gttgcagacg aggtaggcgg
      781 tgggcagttg cagcgtgccg tctgaaaaac ggcggcgacc tttgtagtcg ttcatccacg
      841 cgccgccgcg tttgccttcg cgtgcgtaca aatccatata aacgccgcct atggtttcgc
      901 cgttttgttg caattcaaaa tagcgcacgt ctttgtgcca gacggggacg gttttttcgg
      961 taaatccgat gccgtagagt tttttgattt gggcgaacag tccgtttaat actttgccga
     1021 cggggaagta ttttttgact tcggtttcgc tgaacgcgta tttggcttcg cgcagttttt
     1081 cgctggcgta gcccaagtcc cacggttgca aatcggcgag gttcaggctt tcgcgggcga
     1141 aggctttgac ttcggcgagg tctttttcgg cgtagggttt ggcgcggcgg gcgaggtcgt
     1201 gcaggaagtt taaaacttgt tcgggcgtgt ccgccatttt ggttgccagc gacaattcgg
     1261 cgtagttttt gaagccgagc agtttggcgg tttgcagggc gtttgcgagc gtgcggtcga
     1321 tgttggcggt gttgtcgaat ttgccgtcgt ctgacagttc gctggcgcgg gtaacgtagg
     1381 cgcggtagat ttgttcgcgc agttcgcggt tgtcggcgta ttggatgacg gcgaggtagt
     1441 gtggaatctg caagccgatt ttgtagcctg ttttgctttc gctttgcgcg gcggcggcaa
     1501 acatggcgag cgcgtcttcg ggaatgccgg caagcggtgc ggcatcgtca aagtaaatgc
     1561 cgaacgcgtc ggtcgcgtct aggacgtttt gggagaattt ggcggaaagt tgcgcgcctt
     1621 cggtttgcag ttttgccagt tctgcctgct gttcgggcgg cagttccgcg ccgctgagga
     1681 cgaaatcgcg cagatcgtgg ttgagtttgg ttttttgtgc gggggagagg gtgtcgaatt
     1741 cgggggaatt tttgatggtt ttgaagcggt tgtacagctc gatgtcttgt ccgatttcgg
     1801 tgaagaagac ggtgatttcg ggcatcagtt cgttatagac ggcgcgcagt tcgggcgtgt
     1861 cggcgacgga gttgaggtgc gacaccacgc cccaaatcct gccgacgcgt tcggtgatgc
     1921 cggtcagggg ttcgacagtg tttgcccagc cggtgtgcgt ttgggctttg atggcggcga
     1981 tttgttcgcg cgcttcggca atggcggttt gcagggcggg tttgatgtct tcggttttga
     2041 tttgatcaaa acggggttct tcgcccaaat ggagcagtgc gttgtcagtc ataagatggg
     2101 tttcctttgc gtgttgtgct tcagacagca tatcggcaat atgccgtctg aagccggaaa
     2161 tggggtcggt cggggaaagt taaagtgttt cggagccggc ggggtaattt aacaggcggt
     2221 attgtacgcg caaaaattcg tccataaccg cctgttggat ttccagccgc tttgaaacgg
     2281 gggaggcgaa gcggacgatg atgcggtatg ccttgtcatc gtacggcacg cgggtaacgc
     2341 gcggtctggc ggcgggcgtg ataaacagtt tttccgcctg cacgttttcc aaatgccgtt
     2401 ggatggcggg gatgtagggc gcgcacaagg gctcgagtac ggctttcaga cggcatacgg
     2461 cttcatccga atccaaatgg atgggaacag ggatttcgac cgtatggatg acgtagtcgc
     2521 ccaaaatatt gtcgcggcgc acggggtggc tcaacaacag gctgttgggg aaagaaacgg
     2581 tggttcccgc aagctgtccg accaaggggt tcggaccgac ctgcatcatc agcgtgttca
     2641 acaggttgat gtcgaccacg cgcccgcgca ggccgttgat ttcgatatag tcgccgaccg
     2701 agtattgctg ggtggcagac cttaaaatac tgcccgacag acacataatc agttccttcg
     2761 tcgccacgac gaccgccgcc gccaccgcaa acatcgacaa agccagcgtt tggatttgcg
     2821 ccgaccagat aaatgccagc gaaaacagca ccaaaagcag cgttatattg cggctggcaa
     2881 ccaaaaaccg ccgcttgctt tcgatgccga aatccggatg ccgtttgaag tggatattca
     2941 acagaagggc gcgcgccagc agcaaagccg caaccgccgc cacggattcg accgcctccg
     3001 cacgtatcgg gacggcaccg agccaagtgt ccaacatatt ccatatttcc atttccgcgc
     3061 ccctgtccga aaagtatagg gcgtagattt tagtggcaaa aaaacgcttt tgccacttta
     3121 cgggaaaatc cccgacctga tgccggaggc aaacgtaccg tttcatcttt tcccggcggc
     3181 acactgatgc ggataacgct gaattaggcg gcaaaatcgg ctataataca caattatata
     3241 tgccgtcccg cccgcttttt ccacaaggtg cggacttgtt ttggcggcat cgcaaaatct
     3301 tttcaaaatc cggcaaaaaa tatgactgaa caaaaacacg aagaatacgg cgccgacagc
     3361 attcaggtgc tcgaaggctt ggaagcggta cgcaaacgcc ccggcatgta catcggcgac
     3421 acgcaggacg gcagcggcct gcaccatatg gtgtttgaag tattggacaa cgccattgac
     3481 gaagcactcg ccggacattg cgacaaaatc acggtaacga tacacgccga ccattccgtc
     3541 agcgtcgccg acaacgggcg cggtatgccc accggcatcc acccgaaaga aggacgctcc
     3601 gccgccgaag tcatcatgac cgtattgcac gcgggcggta aattcgacaa caacagctac
     3661 aaaatctccg gcggcctgca cggcgtgggc gtgtccgtcg tcaacgcgct gtccgactgg
     3721 gtaacgctga ccatctaccg cgacggcaaa gaacacttcg tccgcttcgt gcgcggcgaa
     3781 accgaagagc cgttgaaggt tgtcggcgat tccgataaaa aaggcacgac cgtgcgcttc
     3841 ctcgccagtg cggaaacctt cggcaacgtc gaatacagct tcgacatcct cgccaaacgc
     3901 atccgcgaac tttcgttcct gaacaacggc gtggacatcg aattgaccga cgagcgcgac
     3961 ggcaaacacg aaagcttcgc cctttccggc ggcgtggctg gattcgtgca atacatgaac
     4021 cgcaaaaaaa cgcccttgca cgaaaaaatc ttctacgcgt tcggcgagaa agacggcatg
     4081 agcgtcgaat gcgcaatgca atggaacgac agctatcagg aaagcgtgca gtgcttcacc
     4141 aacaacatcc ctcagcgcga cggcggtacg cacctgaccg cgttgcgcca agtgatgacg
     4201 cgcaccatca acagctacat cgaagccaac gaagtcgcca aaaaagccaa agtggaaacc
     4261 gccggcgacg atatgcgcga aggtttgacc tgcgtgttgt ccgtcaaact gcccgacccc
     4321 aaattttcgt cccaaaccaa agacaaactg gtttccggcg aaatcggtcc cgtcgtcaac
     4381 gaagtcatca gccaagccct gaccgacttc ctcgaagaaa accctgccga agccaaaatc
     4441 atcaccggca aaatcgtcga tgccgcccgc gcgcgcgaag ccgcccgcaa agcccgcgaa
     4501 atcacccgcc gcaaaggcgt aatggacggc ttggggctac ccggtaaact cgccgactgc
     4561 caagaaaaag accccgcatt gtcagaactt tatctggtcg agggcgattc tgcaggcggt
     4621 tccgccatgc aggggcgcga ccgcaaattc caagcgattt tgccgcttaa gggcaagatt
     4681 ttgaacgtcg aaaaagcgcg ctttgaaaaa atgctcgcca gtcaggaagt agccgctttg
     4741 attaccgcgt tgggcgcggg catcggcaaa gaagaattca atgccgagaa gctgcgttac
     4801 caccgcatca ttatcatgac cgatgccgat gtggacggtg cgcatatccg caccttgctt
     4861 ttgaccttct tctaccgcca aatgcccgaa ctggtcgagc gcggctacat ctatatcgcc
     4921 cagccgcctt tgtataaagc gaaatacggc aaacaggaac gttacctcaa ggacgagttg
     4981 gaaaaagacc aatggctgct cggtcttgcc ttggaaaaag ccaaaatcgt ttcagacggc
     5041 cgcaccatcg aaggcgcaga acttgccgac accgccaaac aattcctgtt ggcaaaaacc
     5101 gtcatcgaac aggaaagccg cttcgtagac gaactcgtcc tgcacgccat gctacacgcg
     5161 tcgcccattg atttgacgtc gtctgaaaac gccgataaag ccgttgccga actttccggt
     5221 ctgcttgacg aaaaagaagt cgccctcgaa cgcatcgaag gtcatgaagg acaccggttc
     5281 atcaaaatca cgcgcaagct gcacggcaac gtcatggtca gctacatcga acccaagttc
     5341 ctcaacagca aagcctacca aaccctcacc caaaccgccg ccgcgctcaa aggcatggtc
     5401 ggcgagggtg ccaagcttta caaaggcgaa aacgggtacg acgcggacag ctttgaaacc
     5461 gctttggaca tcttgatgag cgttgcccaa aaaggtatgt ccatccaacg atacaaaggc
     5521 ttgggcgaga tgaaccccga acagctgtgg gaaaccacga tggatcccgc cgtgcgccgc
     5581 ctgttgaaag tgcgcatcga agatgccatt gccgccgacg aagtattcgt taccctgatg
     5641 ggcgacgagg tcgaaccgcg ccgcgccttt atcgaaaaca acgcgctgat tgcccaaaat
     5701 atcgacgcat aagtgccgtt ttaaaaaagg agacgggcat cgtgccgcgt ctccttttgg
     5761 tttgtcaaac ggaagccatg ccgtctgaaa ccgcctttcg gagcaaaacc atggtcagca
     5821 tttttgatat tttcaaaatc ggtatcgggc cttccagttc gcatacggtc ggcccgatga
     5881 aggcagcagc cgcctttgcg gcaggtttgg atgcacaggc tgttcgcatc gtcatcgaca
     5941 tttacggctc gctcgccctg accggatacg gacacggtac atttgacgcg ctgatgctcg
     6001 gtttggaagg cagcctgccg cacgacatcc cgcttgtcgg cattcccgaa cgcctcgaac
     6061 gcatccgcac gcagcacatc ctccggctca acgggcaaga aatccgcttc atccccgacc
     6121 gcgacctgaa catactcggc aatcaagtgc tgcccaaaca ccccaacggc ctgcgtttta
     6181 ccgcctatgc ttcagacggc acggtattga atgaacaggt ttattattcg gtcggcggcg
     6241 gctttgtcgt taccgaagaa gattttgacc ggcaggcgga aacggaaaaa gccgttccct
     6301 atccctatac cagttgcgcc gaactgcttg cccgatgccg tctgaaccgg ctcgacatct
     6361 ccgaagtcgt gttggcaaac gaagccgcgc ttgccggatg cggcgaagcc gaaatccgcc
     6421 gccgcgccgc tgccgttgcc gaggttatgg aaggctgcat caaacgcggc ttgggtgcgg
     6481 acggcgaact gcccggcgga ttgaacgtcc gccgccgcgc cccgcagctt gccgccaagc
     6541 tcaaagtcct gcgcgaaacc gaaatcgtca acacccagct ctggccgatg gtgtacgcca
     6601 tggcggtcaa cgaagaaaac gccgccggcg gacgcgtcgt taccgcaccg accaacggcg
     6661 cggcaggcat cattcccgcc gtattgcact atttccgcaa gttcaatccg cacgccacac
     6721 aggaacgcgt cgaaaacttc ctgctcaccg caggcgcaat cggcatcctc tacaagacca
     6781 acgcctccat ttccggtgcg gatgtcggct gtcagggcga agtcggcgta gcgtgttcga
     6841 tggcggcggg cgcatacgcc gaagtcatcg gcggcacgcc caaacaagtg gaaaacgccg
     6901 ccgaaatggc gatggaacac catttggggc tgacttgcga ccccgtcggc ggactggtgc
     6961 aaatcccctg catcgagcgc aacggcatcg ccgccgaaaa agccctcaaa ctcggcacgc
     7021 tcgcgctttt ggaagacggc acggacaaaa aagtctcgct cgacgaagtc atccgaacca
     7081 tgctgcaaac cggccgggat atgaaggcga cctacaaaga aacctcgctt gccggactcg
     7141 ccgccacgct ccggaaaaaa gccgtccccg tatccgtgcg cgtggtcgag tgctgaggat
     7201 ggacagaaac gaaaatgccg tctgaaaaca gttttttaga cggcatttca tttatttctt
     7261 gcggctttac cgtttcagtg ccagcgtcaa cacgcccgcc gtaaccagcc ccaagcctat
     7321 ccattcctgc gtgttcgggc gttcgtctaa gaaaaccacc gccatcagga atgcaaaccg
     7381 gcgttgtttg cagcatccgg caag
//
LOCUS       DAXHRI010000097         7353 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXHRI010000097 DAXHRI010000000
VERSION     DAXHRI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.097"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(199..1011)
                     /gene="murI"
                     /locus_tag="ACFM75_001465"
     CDS             complement(199..1011)
                     /gene="murI"
                     /locus_tag="ACFM75_001465"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC6255997.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1213..1674
                     /gene="tsaE"
                     /locus_tag="ACFM75_001466"
     CDS             1213..1674
                     /gene="tsaE"
                     /locus_tag="ACFM75_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC6255998.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            1671..2921
                     /locus_tag="ACFM75_001467"
     CDS             1671..2921
                     /locus_tag="ACFM75_001467"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6255999.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRNIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYLNTSVLKRG"
     gene            complement(2979..4121)
                     /locus_tag="ACFM75_001468"
     CDS             complement(2979..4121)
                     /locus_tag="ACFM75_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC6256000.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERNIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKYYAG
                     WLAAMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVEGSNR"
     gene            complement(4170..4592)
                     /locus_tag="ACFM75_001469"
     CDS             complement(4170..4592)
                     /locus_tag="ACFM75_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256001.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(4585..5394)
                     /locus_tag="ACFM75_001470"
     CDS             complement(4585..5394)
                     /locus_tag="ACFM75_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6256002.1"
                     /translation="MTRDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEYAGSVWMMVQTREQWRQAQEKGADVVVWRVGNAAQ
                     AAAVDEVLKHGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(5468..5845)
                     /gene="acpS"
                     /locus_tag="ACFM75_001471"
     CDS             complement(5468..5845)
                     /gene="acpS"
                     /locus_tag="ACFM75_001471"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC6256003.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     IRHVNLSMADEGDTVLAFAVAEK"
     gene            complement(6045..6812)
                     /locus_tag="ACFM75_001472"
     CDS             complement(6045..6812)
                     /locus_tag="ACFM75_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6256004.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(6822..>7353)
                     /locus_tag="ACFM75_001473"
     CDS             complement(6822..>7353)
                     /locus_tag="ACFM75_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC6256005.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1791 a         2026 c         1782 g         1754 t
ORIGIN      
        1 atttgagatt gcggcattta tcgggagcaa cagaagccac tctgccgtca ttcccacgaa
       61 agtgggaatc caggacgcaa aatctcaaga aaccgtttta cccgataagt ttccgcaccg
      121 acaggtctag attcccactt tcgcgggaat gacgggattt taggtttctg taatctcggg
      181 cagctttccg tcatcgtttt aacccaaaga caccatttca atctgctcca tcgtcctgcc
      241 caaaaaacgc tcgccgatgg ttctgaattt caaaggaata tcgctgacgt aaaaacggta
      301 gtcgggattg ttgttgtcgg tattgagcaa cccttcctgt gcaaggacgc gtgcggtttc
      361 ttcggcagtc gtaatcgcgg aatcgaccag tgcgacatta tgcgcctccc tgccgattaa
      421 gggcttgagc aagggaaagt gtgtgcagcc caacaccagc gtatcgatgc cgtctgcaag
      481 caatggtttg aggtattcgc atacggtcag gcgggtaact tcatgttcca accaaccttc
      541 ctccaccaaa ggaacaagca acggcgcagc ctgcgtgcgg acgagcgtat cggggttgtt
      601 cctgtggatg gcgcgcgcat aagcattgct gttaactgtg gtattggtgg cgataatgcc
      661 gattttattg ttgcgcgtcg ttgccagtgc ggctttcgcg ccggcggaaa tcacgtccaa
      721 aaccggcata ttgcccgtct tttgacggat tttctgcccc gccaccgccg caatcgtatt
      781 acacgcgata accatcgcct tgacatcgtg ttccaataaa aaatcgacaa tctgcatcga
      841 gaaattttcg atggtcgcct tagatttcgt cccgtaaggc acgcgcgccg tgtcgccgaa
      901 ataaatgatg ttctccatcg gcagccgctc catcagcgcg cgtacattgg tcaaaccgcc
      961 gattcccgag tcaaaaacgc cgatgggtcg ctgcctgccg atattttcca tcctttttcc
     1021 aatctgtcct aaacatacaa tccgcgtatt gtacacgcgc cgctttttct tgacagccgt
     1081 tgccgtctga aagcagaacg cggatttggc tgttataatg ctcggacaag aagcatccgc
     1141 ccctcgggtg caaagtttgc aaaacctgca aaactgccca taatacccta gaccttacaa
     1201 caaaccgttt ccatgtctga tttaccctct atctcccgat tccttgccga cgaagccgcc
     1261 acactcgatt tgggcgcggc gtggtcttcc cgtttaaacg caccgctggt catttatctc
     1321 gaaggggatt tgggtgcggg caaaaccacg ctgacacgcg gcatcctgcg cggattgggt
     1381 catcagggcg cagtcaaaag cccgacctac gccatcgtcg aatcttatcc gctggaacgc
     1441 ttcaccttgc accatttcga cctctaccgc ttctcgttcc ccgaagaatg ggaagatgcc
     1501 ggattggacg aactgtttgc cgcaaacagc gtctgcctga tcgaatggcc gcaacagggc
     1561 ggggaattta cgccgcctgc cgacatcacc gcaacattga cacacgacgg cgacggcaga
     1621 aaatgcctgc tgaccgccca taccgaacga ggacgcgaaa gcctgccgct atgaccaaac
     1681 tgacacgaag acaaatcatc cgccgcaccg ccggcacact gttcgcccta agccccatcg
     1741 catccgccgt tgccaaaacg gtacgcgcac cacaattcac tgccgcacgg atatggccat
     1801 cgcacaccta cacccgcctg acgctggaaa gcaccgccgc gctcaaatac cagcacttcg
     1861 cgctcgacaa tccgggcagg ctggtcgtcg acatacaaaa cgcaaacatc aataccgtat
     1921 tgcacggact gtctcagaag gttatggcgg acgacccctt tatccgcaat atacgcgccg
     1981 gtcagaacac gcccgacacc gtccgcctcg tcatcgattt gaaacagcct tcctacgcgc
     2041 aagttttttc cctccccccc gtcggcggct ttaaaaaccg cctcgtcgtc gatctctatc
     2101 cgcaagggat ggatcccgac gatccgatga tggcgctgct taacggcagc ctgaacaaaa
     2161 cccgtcgcgg ctcccctgaa gccgacctcg ctcaaaacac tcccgcacaa accgaacgcg
     2221 gcaaaaacgg gcgcagaccc gtcattatga tcgaccccgg acacggcggc gaagaccccg
     2281 gcgccgtcag cccgggcggt ttgcaggaaa aacacgtcgt cctctccatc gcccgcgaaa
     2341 ccaaaaaaca attggaagcc ttgggttaca acgtctttat gacgcgcaac gaagacgtgt
     2401 tcatcccttt gggcgtgcgt gtcgccaaag ggcgcgcacg gcgggcggac gtatttgtct
     2461 ccatccacgc cgatgccttc accagcccct ccgcgcgcgg aacgggggtc tatatgttga
     2521 acaccaaagg cgcgaccagc tccgccgcca aattcttgga acaaacccaa aacaatgccg
     2581 acgcggtcgg cggcgtaccg accagcggca accgcaacgt cgataccgcc ctgctcgaca
     2641 tgacccaaac cgccacgctg cgtgacagcc gcaaactcgg caaactggtg cttgaagaat
     2701 tgggcaggct caaccacttg cacaaaggca gggtggacga agccaatttc gccgttttgc
     2761 gcgcacccga tatgccgtct atcctggtcg aaaccgcctt cctgtccaat cctgccgaag
     2821 agaagctgct gggcagcgaa tccttccgcc ggcagtgcgc ccaatccatc gcctcgggtg
     2881 tccaacgcta cctcaataca tccgtattga agcggggttg attccgaaaa ataaatgccg
     2941 tctgaacatt tttcagacgg catttttgta cactttcctc aacggttcga tccttccacc
     3001 atatcaatca ggaacaggcg gcaatcgagg ttgccgttat aaagcggaat tttgcgtttt
     3061 ggcgacaggc gcatgaattt gggcatttcc cgatctccgg taaacattgc cgccaaccag
     3121 cctgcgtaat atttcttcaa ccacgtcccc aactgcggat aaagcgcctg caaggcgcgg
     3181 acttcctcaa ggcgcacgcc gtagggcgga ttggacacca taatgccgcc cacgccgttc
     3241 ggacggacgg attgcgcgtc ggcaacgctg aaggaaacga tgtcgtccac cccggcactg
     3301 cgtgcgttgg ccaatgccgt ctgaatgatg cggcggtcgc tgtcgctgcc cgaaatcggt
     3361 gcaaaagccg gtttaacctg cgcttcggca cggcggcgca aatccgacca cagcgtttta
     3421 tcgaaatttt gcagtttttc aaaaccgaaa cggcgcatca tacccggcgc gcggcgggcg
     3481 gcaatccaag cggcttcgat agcaatcgtg ccgctgccgc aaaacgggtc ttgaaacggc
     3541 tgcgtgccgt cgtagcctgc cgagagcagc aatccggcgg caaggttttc gcgcagcggg
     3601 gcttcgccgg tatcgagacg gtagccgcgt ttgaacaggg cttcgcccga agtgtcgatg
     3661 aagatttcga cattgcgttc gtccaaaaag gcgtggatgc gaacatcggg cgcggctttg
     3721 tccacgctcg gacgtgcgtc gtaaatgtcg cggaaagcgt cgcagacggc atctttgacg
     3781 gtcagtccga caaattggat gctcttaacg ttggcacgct ttgcctcgac tttgactttg
     3841 aacgtctgct gtaaagtaaa ccaattaaac cagttgatat ttttggcgag tttgtagatg
     3901 ttgcgctcat tgcggtatgt ccctttggtc aggcgcagca ggatacggct ggcagtacgc
     3961 gaatgcaggt tggcggcgta aacctgttcc aatccgcccc ggcaggaaac gccgccgtca
     4021 aacacttgta catcggtaca gccgaggctt tcgagttctt gagataaaac ggtctccaag
     4081 ccgcgcgggc aggtggcgaa aagtgtatat aaagtattca taacgtgtcc tttccggacg
     4141 gatgttccgg caatggcgga ttgaaaggtt tattcggcgg taaatgccgt ctgaaatgtc
     4201 cggctgccga ttgtaatgtc cgccgtaaaa tcgcgcctgc cttcgacaca gatgggcagg
     4261 cggatgcgta ctgcctgcca agtccccgac ggttgccgct cgaacatata gcggttgaaa
     4321 cccatatcca tatttttcat actgaagctg atgctgacct gttccgtgcc gacgggcgcg
     4381 tgttcgatat aaatatcaaa cggttttttg gttgaaacgg cggcggcgcg gacgcggctt
     4441 ccgtccggca gcgtgcaacc ctcggtcaaa tcgcattgcg ccgccacggc ttgcggctgc
     4501 tgcgcctgcc accattgcaa caaaacgagc ttgacggcgg caaaggcaat cagcagcagt
     4561 gcggcaagca gtaatttacg gtttttattc atgattcttc cctattgttt catcccttac
     4621 caccacgtgc gcccccaatc cgagccatag ttttccataa cgtgcaaccg tctgtccgtt
     4681 tgctgcaagt acgagcggca cggatacgcc gtgcttcaac acttcgtcta ccgctgcggc
     4741 ttgtgcggca ttaccgactc gccagacgac cacatccgcg cccttttcct gcgcctgccg
     4801 ccattgttcg cgggtctgca ccatcatcca cacgctgccg gcgtattcgg gtctgtcctg
     4861 ccattgcgcc gcctccatca aaacgttcgc accgcttccc tctgccgaac ccaaaggcag
     4921 gacgcggtac gcgcccatac tgttttctcc gtgcaaaccc gttttcaggc tgccgtacaa
     4981 acggcgcggg acggacagcg aacgcaaaag cgcgccgttg gcgggcagca tgggggcaac
     5041 ggtaaaatca cccgccttct gccaagacca ttcctgccct tcgcgggatt gcggtttgcc
     5101 cgtccattgg tcggggttga cccataggaa tttcaggcag acgcgggcgt gttcgtagga
     5161 atggattttg gtcagccaag gcgtggcggc gaggatgcgg atgccgagtt cttcttcaaa
     5221 ctcgcgttgc aaggcttgga agtcggtttc gcccgcttcg accttgccgc cggcaaattc
     5281 ccaatatccg gcatagggtt tgccttcggg gcgcgagctg agcaggtagt tgccgtctga
     5341 atcgagcagg atgccggcaa cgacgcggat aaggggtcgg gtgtctcggg tcataggaaa
     5401 gatgggaaaa gtttttccga acattagaaa agggcggaac ggtcaatccg gcccggcggc
     5461 tgccgcctta tttttcggca acggcaaacg ccaataccgt atcgccttca tccgccatac
     5521 tcaaattaac gtggcgtatg ccttgttcct ccagccattt ggacagggcg gggccgtaga
     5581 aaaattcggg cttgcccaat gcgtcatgcc cgatgccgat gttgcggaag gaaaccgcgc
     5641 cgcgtatgcc cgtgccgacg gctttggcga aggcttcttt ggcggcaaag cgtttggcga
     5701 ggtagttgac gggtttgccc gcttgcggaa attcaagcag ctcttccgga gtgaggatgc
     5761 gcccggcaaa cgcctgtccg aattttttgt ttaagcggat gatgcgcttg agggaaacaa
     5821 tgtctgtgcc gatgccgtaa atcatatttg cgctccttcg cccttggttg cgggtagtga
     5881 tgaatgtgat gatgaatgcc gtcaatttct tggggcgtta aaatggcgtg aatatccact
     5941 tttggggttt tgtcggtaac aattttcact gtaacctttt gcatttgaaa ttctaaaaca
     6001 tcgtgccaat tatttaataa ggagttaagg catttaataa tgaattaaag tttgattaaa
     6061 cccgaatgcc cgattttcag agccaatacc aataaaacaa taaaaattaa cattttaaaa
     6121 aaatttgggc taatcttagt ccttaaccga attccaacat acaatccaat aacagacaat
     6181 acggacagta aaaatattaa accgtattca ctcttattta ataaccaata ctggtctctt
     6241 agcatatata tttgaacaat tttcgccaaa agatagcata gattgcttga ttttacgata
     6301 cgatttttat tttctgtttc gctaagcaaa aatattaaca atatgggaga catggcattg
     6361 gttgaaccgc cgatgatgcc tgccaaaaac ccaaaaagaa ccatattctt attattggca
     6421 actacttgaa tattttttgc ttttgcacat acatttaaaa taccattgac agaataatac
     6481 aatgtaatga ttgccatcag taaaagcagc caagacactg gaagtatcaa aagcaacttc
     6541 acccccaaaa tgctgccaac gacgctgccg atagcaagca atttataggt ttttaaataa
     6601 taaacaatct cttgccaaaa accctttttg ttattgctgc atagaaccaa caagctcatt
     6661 aacaggcttg gtaatgccac caaggcaaca accttagaca atggcatgat aaaagccaat
     6721 gcggttgtac cgagcatcgg aaatcccatg cctgtaattc cgtgcagtat tgcggcagca
     6781 acaaaaacga tagattgcat tatttcttgc ataaccgtcc tttatacagt aattcttgct
     6841 ctaatataat gcctgttttt atccgtataa tcagttctgg catgcaagcc attgacattg
     6901 tcaataataa tcaaaccatc atcttgtgca gaaaattctt gaatatcact cgcatttttt
     6961 ataacattta caagataatc aagtgcaagt accatttcct ttgatacttt atttctattt
     7021 tcttcaatac ctttatagat acaatcactt ctaaatctaa tcatttgagt attgaccgat
     7081 aagatattac cccatctcac accttgtttt ttatcaaatg atgctggtgt tttaaatgga
     7141 tataaattgc ccgttaatgt ttttaagtgt tttttaccgg tttctgtttt agataactga
     7201 ttgacaatat ctgatgaact taaaaatagg gaattacctc catcattggc tgattttact
     7261 acatacataa ccaaataact ttccgggttt tcactaaaag atgaatcact atgaagtgga
     7321 cattctccaa catcttccga gaaagttata tca
//
LOCUS       DAXHRI010000098         7334 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXHRI010000098 DAXHRI010000000
VERSION     DAXHRI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.098"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            201..413
                     /locus_tag="ACFM75_001474"
     CDS             201..413
                     /locus_tag="ACFM75_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC6256006.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            561..2144
                     /locus_tag="ACFM75_001475"
     CDS             561..2144
                     /locus_tag="ACFM75_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC6256007.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDLLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYVAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2226..3323
                     /locus_tag="ACFM75_001476"
     CDS             2226..3323
                     /locus_tag="ACFM75_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC6256008.1"
                     /translation="MNHTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3437..4552
                     /locus_tag="ACFM75_001477"
     CDS             3437..4552
                     /locus_tag="ACFM75_001477"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC6256009.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4677..5132
                     /locus_tag="ACFM75_001478"
     CDS             4677..5132
                     /locus_tag="ACFM75_001478"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC6256010.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDYIIAMDGNNLSELERTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5203..5679
                     /locus_tag="ACFM75_001479"
     CDS             5203..5679
                     /locus_tag="ACFM75_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC6256011.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5667..6593
                     /locus_tag="ACFM75_001480"
     CDS             5667..6593
                     /locus_tag="ACFM75_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6256012.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6658..7215
                     /locus_tag="ACFM75_001481"
     CDS             6658..7215
                     /locus_tag="ACFM75_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6256013.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNAVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         1919 a         1812 c         1913 g         1690 t
ORIGIN      
        1 atgcgccgat tgccaaggcg atgatgctca accagggttt ttgcgaagcg ttcattgttg
       61 tcgtcctgat gggaattgcc gccattatgc gcaagcgtcc gtctttttcc aaaccgccgc
      121 accgtttccg cgtttgccca agttcacgcc ccgacttaca ataacgcccg tcagattgca
      181 gttccaacga aaggaaaatg atggaaaccc ttatcctcga catcggcggc atgagctgcg
      241 gcggctgcgt caaaagcgtt acccggatat tggaaggcgt aaaaggcgtg gcaagcgtcg
      301 aagtcagcct tgaaaacaaa agtgcgaccg tcggatacga ccccgcgcaa accgatgcag
      361 gagcgttgat tgaagccgtt gaagacggcg gctatgatgc cgcgttgaaa taaagcggca
      421 aaaatgccgt ctgaagcctt cgcgcctccc cgatgccgtc tgaagtacgc tccgcgcctt
      481 cagacggcat ttgatttagt tgaacgggta ggatgaattg gttaaacttg cgggcaatag
      541 tgccttcaga caaacaaaat atgccgaaca aaaagaaaaa agccgccttt gcgcgtgctg
      601 caacggagaa cggtgtgccg gacgtgtcca acatcaaaaa ttcatatgat gatttgatgt
      661 atgaatccaa agcattttcc caaaccgcca tcaacgcctt ggaggctcgc gcccgcatga
      721 tgggtttgca gcccgcgccc gccgccaatg ccaaggtctt ggagctgggc tgttcgatgg
      781 gcggcaacat catcacgcag gctctttatt acccggatgc ggaatttgtc ggtatcgact
      841 tgtccggcag gcaggttgcg cagggcaacg ccatcattga aaaaatgggc ttgaaaaatg
      901 tgcgtctgga agaaaaagat attttgaccg tcgatgagtc attcgggaag tttgactata
      961 tcatcgtcca cggcatttgg tcgtgggtgc ctgacgcagt taaagacaaa attttttcga
     1021 tttgccggaa caacctgacc gaacacggca ttgcctatat ttcatacaat gtttacccgg
     1081 gctggaaacg gcaggagcag ttgcgcgaaa ttatgtactt tgccggcagg gatctgcttg
     1141 aagaaccctt ggaagcgcgg acgcggaaag gcttggacgc gctcaaggcg ctggcggaaa
     1201 ttttggaaaa cgacaaaggc ttgggcggcg gcggcaaact tccggcgatt caaaaaatat
     1261 tgaatcacaa tacttattat gtcgcacacg aatatatgga aatattcaac gacccgattt
     1321 acgttaacgg cttcatcgaa tgggcaaacc gccaccggct ggcgtatatc ggggataccg
     1381 atttgcacgt gtcttttgtt tcttggatgg cggagcatac gcgggagcgg attttggcat
     1441 tggcagggga tgattatatt gccaaagaat tttatagcga tattttatcc gaccgccaat
     1501 tccgccgttc gcttttatgc cgtgaggaag tcggggacaa tatcaggcgt gatgagtcgg
     1561 ttgccgtcga agtgatagaa agtttgaatt tccgcccggc aagaggggaa acgatcaact
     1621 ttgatgaaaa cgataccctg ctttcgggca tacgcgatgt gatgaaaacc ggagagccgt
     1681 ttaaaacgga agatgttgcg gaaaacctcg cccgccgatt tcccggtctg gaatttgacc
     1741 gtatgaaaat caattcccag cttttattgc aaaccattct cgggcgtttt tctgtttcat
     1801 cagacaatgc gggcaaacca ttttttgaag accataaaac ctatgtgccg gcgcgtttta
     1861 cagattatgt cgccgccttt gtggaacacg gcgcggaagc gtttgttcgg cctgccaacc
     1921 gttacaacga aagcaccccc tcattcggat acgggcattt gtacattatg cggcaattat
     1981 cgcggccgac gagcaaacag gcattgattg aaacggttgc cgaaaacttg aacatcgtca
     2041 gcaccacgcc cgacggtttg acattccatc cgcctgccga agtgtatgtg gaagaaatat
     2101 tggcagactt ggcagacagg cattttctcg tttcggcgga ttgacgggca agtcccggcg
     2161 gcaatccgtc cgatatattt tcagacagga aagacacaca ctgcgaaaag cggtacacga
     2221 catatatgaa ccatacattt accttgcccg atacgcgccc gtatccccaa aatccgatta
     2281 agaaccacct gctgctcaat gcctaccagt tggcgcacaa ttcttcccag gcttcgcgca
     2341 aactctcgtc cggccaactt caaaccgaaa tcagggggat gcttgagcaa aaccactata
     2401 tcaacctttc cctcgcgctg acgatgtcgc ccgatgccgg aacttatgcc gcgctgcttt
     2461 ccagtgtgaa cgcggtactc gattgcgaga aagaaggcga agtgcagtgg ttcgccctgc
     2521 cggtcgtgct ggtgtccggc tgcaagaaag aacgggcaat cgagatgaag ctgccgacgg
     2581 aggcattgtt tgcctgtttg caaaactatc cgcacctgcg cgcgttgacg caagagacgc
     2641 aatggctgcc ttatcttgta cattcctccg atttgagtgc ggtcgcgccg gatgagtggt
     2701 ggcgtgccaa acaaaatacc gaagcggcgg cgcaacactt acgccgtttc gccccgcgcc
     2761 ctttgctttt gcccgaaggg cagtccgtcc acgtcgttta cgcgctgggt ttcggcagcg
     2821 gcaaagttca gacggcattg ggtcagaacc ttcttcaggc aggtctgccc ttgatgcagg
     2881 tatggcagga aaatcttgca tcggaaggcg ttacgctgtt tgccaatccg ctttccccgg
     2941 attctccggt acgcgcgctt tccgacggca gccacacgcg ccagcgcatg gcgatggatg
     3001 tgtttgcggc aaacgcgata cgcgccgtcc gtatgcagag tccgcgcgtc ggcgtggtcg
     3061 cagcggcaaa ggcgggcgga cagattttat tcgggtttaa tgcgaccgac ggcgcgtttg
     3121 aagtcgtgcc gcaggtgttt tcgtggcagc tttcctttac cgacaacatc gccgtcatcc
     3181 agcaaaattt cctcgacctg atggcggagt gccgcgtgga acacgtttac ctgttgcaca
     3241 atcccttggg cgaacaggaa agcatcccga gctatgcgga agcattgaaa cgggaagggc
     3301 acaatccgtt tttcagcgca tagtgatttg tagtagtgat aggctttctc atttatataa
     3361 taaatcaaaa taaagagcac aacacttttt cattctgtgt tgtgccttga gtgaaacgaa
     3421 aggatgaatt ataaatatga agattgtaat tgcaccggat tcgtttaagg aaagcttgac
     3481 agctcaacag gtagctgaag caataaaaag aggcttccaa caatcgatag cagatgtgga
     3541 atgtctcctc tgtcctgtcg gtgatggggg agaaggcact gtagatgcta tccgacattc
     3601 tcttgaccta gaagaaaaat gtcttcaagt gacaggacct tttggacaaa aagaagtcat
     3661 gcgctatttt caaaaagaac aactagccct attcgaagtt gctgatttgg ttggtcttgg
     3721 aaaaatcccg ctagagaaac gaaatccatt acaaatccaa actcgtggta ttggagaatt
     3781 gattcgccac ctcattagtc aagaaattaa agaaatctat attggcgttg gcggtacggc
     3841 cagtaatgat ggaggtattg ggattgctgc tggtttaggt tatcaatttt atgatgagga
     3901 tggaaatacc ttgcccgctt gcggtcaagc cttactaaac ctagcttctg tttcaacaga
     3961 aaatcgctat gaaattcctg aagatgttca cattcgtatt ttagcagatg ttgtgagtcc
     4021 cttatgtgga caccaaggtg cgacctatac gtttggcaag caaaaagggt tggattctac
     4081 tatgtttgag gccgtagatc aggcaataca agatttttat gaaaaagtct cccctgcaac
     4141 attaaaactt aaaggagcag gagctggtgg aggcatcgct ggtggtttgt gcgcctttgc
     4201 tcaggcaagt atcgtatctg gaattgacac ctgcctggac ttgattgact ttgataagaa
     4261 agtgtcagat gttgacttgg ttatcgttgg tgaaggaaga ctagatcgtc aaagtttagc
     4321 agggaaagcg cctattggtg tagcaaaaag aacccctgtc ggagttcctg ttgtcgctat
     4381 ttgtggcagt ctcgttgaag atttgccttc cctgccattt gaaaatatcc aagctgcctt
     4441 ttctattttg gagaaaagtg aacctttaga agatagtttg aaaaatgcca gtctctattt
     4501 ggagcatacg gcttctaata ttggacactt attaaatatg cctaagattt agccaaacca
     4561 tttcttccag atggatgttt tggctggttc tgccttatgc ggttacgatt aaccgaccga
     4621 tgccgtctga aagggtttcc gcttcagacg gcatcgcgcc ggaacaggga ggggatatga
     4681 aaaaaccgaa aatccttttt gtctgcctcg gcaacatctg ccgttcgccg atggcggaat
     4741 acattttgcg ccgccgcgcc gccgaagcgg gcattcccct tgaagcggat agcgcgggga
     4801 catcgggctg gcacgacggc gaagatatgc accgcgagac ggcaaagata ttgaaaaaac
     4861 acggtatcga tgcttcaggc tttaccagcc gcaaaatccg ccaaagcgat gcggcggcgt
     4921 ttgactacat catcgcgatg gacggcaata atttgtccga attggaaaga accttcggca
     4981 ggcggtcgga aaaaatattc aagctgaccg acctgatacc cgaaagcggt tacgaccatg
     5041 tccccgatcc gtggtacacg ggcgattttg aagaaacctt caggcttgcg gatgcgggct
     5101 gtcgggcatt gttggaaaag atttccaaat aaagcaattg aatacaaata taaaaccgcc
     5161 tcctgccgta tcggttgttc agacggcata aacagagaat ttatgaaaac aaatttcaaa
     5221 cagaaaatta tcgaacaggc acgcagcgaa ggcttgcagg taaccgcttt gcgcgagcag
     5281 gtattagata ttgtcttgca gcaaagcggc gtgattaaag cctacaacgt cttgtcgcag
     5341 atgcagcagc aaagcgaggg cgtgcttgca ccgcctaccg cctaccgcgc ccttgatttt
     5401 tgggcggagc agggcgtttt gcacaaagtg gcggcggtca acggctatat tttgtgcagc
     5461 cacgcgcagc acgagtgcga cgaccattgc cacgaccacg aagaagccga agcgcaccac
     5521 agcgcgttta ttttggtctg caccgaatgc ggcacggcgg acgagcaaac cctcagccac
     5581 gagtgggcgg cactgcgcgc aggcgttgcc gaaagcggct ttgcgctgaa agaagaacac
     5641 gttgttttaa ctgggatttg taaaaaatgt cagaaatgaa aaaaaccaaa gtccacctga
     5701 tttcaggttt tctgggaaca ggcaaaacca ccgcgctcaa aagcctgatg gcgcaaaaag
     5761 acccgaacga aaaatgggtc atcatcgtca acgagttcgg cgaaatcggt attgacggcg
     5821 cggtattgag cgacaacggc atccctgtgg cagaaatcgc cggcggctgt ttgtgttgca
     5881 ccgccggccc gcaaatgggc gtaaccgtgc agaaaatgct gcgcgacgcc aagcccgacc
     5941 gcctgatgat tgaggcaagc ggactggcgc acgcagccag cgtcatcgac gaattgaaag
     6001 ccaaaccgct ggacagcctt ttggaaatcg gcgccgtctt taccgtcgtc aatccgcgcc
     6061 agttcatcaa ccccgattac gcgcaacagg cgttgtataa agaccaaatc ggcatctgcg
     6121 acgtattggt cgccagcaaa accgatttat gcaccaccga acagcttgcc gaatttcacg
     6181 acaaagcggc aaaactgttc ccgcccaaag ccaaagtggt cgaagtccaa aacgcacaac
     6241 tcgacatccg gtggctcgat attcccgtca tcgaaaaatc acgctaccgc ctcaaagccc
     6301 tgccggacaa caccatgggc ttccagtcgc aaggtttcac attccccgcc ggacgcgatt
     6361 tcgacggcga aaagctgacc aactttttca acgacctgcc caaaatgacc gaaggactcg
     6421 tccgcgccaa aggcgtgttt caagtattgg gaacgtgggt gtggctcaac tgggtagacg
     6481 gacagtgggg cgcgaaccaa gtctcatggc ggcgcgattc gcgttttgaa ttgattgcca
     6541 aatcgtttga tgcggattta atcgaacaaa agctcaaaga cgcattggaa tagcgttcac
     6601 gcgccttcag acggcatatg aaatgaaaat gccgtctgaa ggtctgagga ggacgggatg
     6661 aaacccaaat tcaaaaccgt tttaaccgcg ctgcttttgg cggtttccct gccgtctatg
     6721 gcggcaaccc atgttttgat ggaaaccgat atgggcaata tccgtttggt tttggacgaa
     6781 tccaaagcac ccaaaaccgt tgctaatttc gtgcgctatg cccgaaaagg cttttacgac
     6841 gacaccgttt ttcaccgcgt tatcggcggc ttcgttatcc aaggcggcgg attgaccgag
     6901 gacttggcac aaaaggcaag cgataaggcc gttgccaacg aatccggcaa cggcttgaaa
     6961 aacactgtcg gcaccatcgc catggcgcgg acgacagacc ccgattccgc caccagccaa
     7021 ttctttatca atctggcgga caatgattca ctcaactaca aaaacggaca atacggctat
     7081 accgttttcg gcagggtcga aagcggtatg aacgccgttt ccaaaatcgc ccgcgtcaaa
     7141 accgccacgc gcggctttta tcaaaacgta cccgtacagc ccgtcaaaat ccgtcgcgtt
     7201 gttgtcgggc agtaacacgc agacagacgt tcagacggcg tcgcccgttt cccaaaaaac
     7261 gccgtttaaa ttaaaaaaat attttaaaac agacagttga tattgacaaa ttcaaaccga
     7321 ggattttaaa atgc
//
LOCUS       DAXHRI010000099         7334 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXHRI010000099 DAXHRI010000000
VERSION     DAXHRI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.099"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..126)
                     /locus_tag="ACFM75_001482"
     CDS             complement(<1..126)
                     /locus_tag="ACFM75_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6256014.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWR"
     gene            complement(201..1544)
                     /gene="argG"
                     /locus_tag="ACFM75_001483"
     CDS             complement(201..1544)
                     /gene="argG"
                     /locus_tag="ACFM75_001483"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC6256015.1"
                     /translation="MNQNHTILQNLPIGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            1669..2469
                     /locus_tag="ACFM75_001484"
     CDS             1669..2469
                     /locus_tag="ACFM75_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC6256016.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFVFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALELPVVRHPEAAGFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEYIEQTYLGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLEGLGAELKNRVN"
     gene            2509..3495
                     /locus_tag="ACFM75_001485"
     CDS             2509..3495
                     /locus_tag="ACFM75_001485"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC6256017.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDMMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIIIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIIGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGDRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            4152..4447
                     /locus_tag="ACFM75_001486"
                     /pseudo
     CDS             4152..4447
                     /locus_tag="ACFM75_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039136685.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4452..4826)
                     /locus_tag="ACFM75_001487"
     CDS             complement(4452..4826)
                     /locus_tag="ACFM75_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6256018.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(4823..4951)
                     /locus_tag="ACFM75_001488"
     CDS             complement(4823..4951)
                     /locus_tag="ACFM75_001488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256019.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            complement(4999..5355)
                     /locus_tag="ACFM75_001489"
     CDS             complement(4999..5355)
                     /locus_tag="ACFM75_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC6256020.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNKKTVIFHF"
     gene            complement(5498..5841)
                     /locus_tag="ACFM75_001490"
                     /pseudo
     CDS             complement(5498..5841)
                     /locus_tag="ACFM75_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6240..6641)
                     /locus_tag="ACFM75_001491"
     CDS             complement(6240..6641)
                     /locus_tag="ACFM75_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256021.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTDKTIKISIE
                     DVIHFRIVDESYFIDTFMDLISEDADRALLHENGGQSFFELLNERYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(6645..>7334)
                     /locus_tag="ACFM75_001492"
     CDS             complement(6645..>7334)
                     /locus_tag="ACFM75_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC6256022.1"
                     /translation="EAQLAAASLLQDSAFAVKDGINSARQWADAHPNITATAQTALAA
                     AEAAGTVWRGKKVKLNPTKWDWVKNTGYETPTARHMQTKALGTVDEIGDTVQQVVKQT
                     SGQKTSGGNPAIDSDPYSPSSVAARIEAGKVRSDLQIKDILSNTTQRSKTKGPAVQYD
                     KVGDYNDALNDFNSLNIRNVQTRPNGTITGNLPDGRAVNVRNDSSGGEPTLEITISNN
                     RKIKIRYGNTR"
BASE COUNT         1971 a         1660 c         1648 g         2055 t
ORIGIN      
        1 ccgccagcct tcgggggttt gtttgttgag gaacgcgctg aaattgacgg ggttgacctt
       61 tgccgcgccg aaaaacaggc tggacaacag gctttcctga taaatgacga ctttgtattc
      121 tttcatagga ttccttttaa cataagggat catcttttca gacgacccct taagttgcta
      181 atgatgcaaa acagcaaacc ttattgctta ttgcccaact gcggcaatac cgaaccttcg
      241 cccagcgaga gcaaaccgct ttgcgagtag atacccagtt tgacgcgggt gtcggtgatg
      301 tcgaggttgc gcatcgtgag ctgtccgatg cggtctagcg gggtaaacgc cgcaccttcg
      361 actttttcca tactcaggcg ttcgggttgg taggtcaggt tgggcgattc ggtgttcaga
      421 atcgagtagt cgttgccgcg ccgcagttcg agggtgactt cgccggtaac ggctttggcg
      481 acccagcgtt gggcggtttc gcgcaacatc aaggcttggc tgtcgaacca gcggccttgg
      541 tagagcaaac ggccgaggcg caggccgttg atgcggtatt gttcgatggt gtcttcgttg
      601 tggatgccgg tcaccaagcg ttcgtaggcg atgtggaaca acgccattcc cggggcttcg
      661 tagatgccgc gcgatttggc ttcgatgatg cggttttcga tttggtcgct catgcctaag
      721 ccgtggcggc cgccgatgcg gttggcttcg aggaagagtt cgacgggatc ggcgtattct
      781 ttgccgttca gtgcaaccgg cacgccttct tcaaagcgca cgctgacttc ttcggggctg
      841 acttcgacgt tttcgtccca aaaggcaacg cccataatgg gtttgacgat tttgatgccc
      901 gagttcaaaa attccaagtc tttggcttcg tgggtggcac ccaacatatt ggaatcggtg
      961 gagtaggctt tttcaaccga cattttgtag ttgaagccgt tggcaatcag aaattcgctc
     1021 atttcgtggc ggccgccgag ttcgtcgata aattgctgat cgagccaagg tttgtagatt
     1081 ttcagcgctg gattggtcaa caggccgtag cggtagaaac gctcgatgtc gttgcctttg
     1141 taggtgctgc cgtcgcccca gatattcaca tcgtcttctt tcattgcgga aacaagcatc
     1201 gtgccggtta cggcgcggcc cagaggcgtg gtgttgaaat aggcgatgcc gccggtggaa
     1261 acgtggaacg cgccgcattg gatggcggcg atgccttcat gtgccaactg cgcgcggcag
     1321 tcgattaagc gggcgttttc cgcgccgtat tccatcgctt ttttaggaat ggcgttgtaa
     1381 tcgtcttcgt cgggctgacc gaggttggca gtgtaggcat aaggcagcgc gcctttgagt
     1441 ttcatccaca acagcgcggc agaggtatca agaccgccgg agaaggcgat gccgactttt
     1501 tgaccgatgg ggaggttttg taaaatagta tggttttggt tcatgaaaaa atcctcgata
     1561 gcttgaaaaa tcaaagccat tatcttataa ataggaaata ttgtcagcca tcatgccgtc
     1621 tgaacgggta ttccgccgtt tcaggcggta tttaaaaccg aaaacattat gaacgcgttc
     1681 aacctcatca tcatcggcga cgaaatctta cacggcagcc gccaagacaa gcatttcgtc
     1741 tttttcaaat ccctgctcga atccaaaggg ctgaagctca atcaggtgca atatctgccc
     1801 gacgaacccg atttgctggt caggcaactg cgccgcagct tttcagacgg tataccgacg
     1861 tttgttaccg gcggcatcgg cgccacgccc gacgaccaca cgcgccaagc ggccgccgcc
     1921 gctttggagt tgcccgtcgt ccgccatcct gaagccgccg ggtttatcga aggcataacc
     1981 cagaaacgcg gcgagccgct tgattcgccg gaacacgccc aacgcctgaa aatggcagat
     2041 tttcccgaag gcgcggaatt ggtgcccaat ccgtttaaca acatcgccgg attttccatc
     2101 cgcgagcatt attttttccc cggcttcccc gtgatggcgc acccgatggc ggaatgggta
     2161 ttggaaactt attacgccga ccgcttcaac caaaccgaac gcggcagccg cagcgtgtat
     2221 gtgttcgagc agcccgaatc gcgcattacg ccgcttatgg agtatatcga gcaaacttat
     2281 ctcggggtgc gttcgtacag cctgcccagc gtcggttgga cgcattcaga cggcacgcag
     2341 gtcaaaccgc acattgagtt cggcatcaaa gccgaaggag aagccgtcaa ccttttggac
     2401 gcggcgtggg cggaagtgtt gcacagcttg gaaggattgg gagcagagct gaaaaatcgg
     2461 gtaaactgaa aaacgtttgc ccaaatcgga cgatatttaa ggaaaaccat gcaataccga
     2521 atcagaagag aaaacgaagc acccgaagca aaaaatgcgg gggaaacctt atgggagcgc
     2581 gatatgatgc gcgaagtgct gctgtccgcc tatcgggaca ggcgcaggga gcggatgtgg
     2641 aaaaacatct ggcgcgccgt cagcaccctg attctggttg ccctgattgc aggcattttc
     2701 cgaaaagacg aagccgcatt gcagttggcg ggcaatacgc cgcataccgc agtcgtcaac
     2761 ctgtacggcg aaatcggcaa cggtgtagaa gaccaggtca aaaaactcaa agacggtatg
     2821 gaggccgcct acaaaaaccc gcaggcaaaa gccatcatca tccgcgccaa cagtccgggc
     2881 ggctctcccg tcgtatccaa tactgccttt gaggaaatac gccgtctgaa agcgcaacat
     2941 cccggcattc ccgtttatct cgtggcggaa gatatgtgcg cgtccggctg ctactacatc
     3001 gcggcggcgg cggacaaaat ctatgccgac ccgtccagca tcatcggcag catcggcgtt
     3061 atcggcagca gtttcgacgc gaccggcctg atggaaaaaa tcggcgtgaa acgcagggtt
     3121 aaaattgcag gcagcaacaa aggcatgggc gatccgtttt cgcccgaaac gcccgaacag
     3181 tcgaaaatct gggaagaaat gctgaccggc atacacggcg agttcatcaa agccgtcaaa
     3241 accggacgcg gcgaccgcct caaattccga caatatcccg acgtgttcag cggtcgcgtg
     3301 tacacaggcg cggatgcgct gaaagtcggg ctggtggacg gactcggcaa tatttacagc
     3361 gtggcgcgag acgtggtcaa agcccccgat gtggtggatt acactccgaa ggacgatttc
     3421 ggcagaatct tgggcagacg cttcggcgca gaactcaaag caagcgtgcg cgaagccctg
     3481 caggccgtcc ggtagcgttg tttacagtat ttctaaggga ataccaaaat aaaaatatac
     3541 acaaaaacac tcacctcccc agtatgctgc cactaagtca gccgcgtcac tatttaccgt
     3601 gcacttaaag ccgtaaaagg caggttgtcc ccgggaggtt ttgtgcatcc tgataccgat
     3661 cggttgttgt tgctcaaagg acagaaggcc gctgataaac gagattacct gtttgtcgct
     3721 attgacgatt tttatactct gccattttgc cggacaaaac cgcagacagt gctgccaagt
     3781 ttctgaccga acatctgtcc gacccctgct tgtacctgat tgagtacgtt tactctgaca
     3841 atgataggta atataaagag ccgtccaaca tgctttcggt gcagcttgtt atgataatgg
     3901 gattggttgg aggcttgccc gatttgcttg tccgcagacc aacggtaagg cggagcgggt
     3961 tatccgtacc ttgatggaga tgtggcatga ggaacagtcg tttgacagtc cggaacatcg
     4021 gcaaaaggag ttgtgctgct ttattaattt aggggctgcc ctagatcact agggaaattc
     4081 aaattaagtt agaattatcc ctatgagaaa aagtcgtcta agccggtata aacaaaataa
     4141 actcattttg ttatcgtggc tatgatgtat tggatgcggg cgaatttagc catttccgta
     4201 tcaatcacag cacacatttt gccgaacgac aaaaccatat taatggaact gagaactttt
     4261 ggaatcaagc aaaacgtcat ttacgcaagt ttaacggcat tcccaaagcg cattttgagc
     4321 tgtatttaaa ggagtgcgaa tggcgtttta acaacagtga gataaaagtt cttgttccat
     4381 tttaaaacaa ttagtaaatc gagtttatcc tagttatcca ggatggtccc atctctttta
     4441 tagatagctg cttaccaagt atccttaatt gctaattcaa gccactcttc aaaactatta
     4501 aaaaattcat ggttttctat attatctaaa ttaatgacaa taaccctagg atttcctgtt
     4561 acatcattac catcaaaata agcttcccat tctccattac tagcgaatgg gataagattt
     4621 aaatcaggca ggatagaatt tctaaatata taagctactt ctgccccttc tgttccataa
     4681 ttctcaaacc accagtgaaa attatgtggc tgaatataag gtattaaaga tgtatcgtta
     4741 gagatacgaa catacttttc aggataacaa aacctttcag gaagcatatt actaggatat
     4801 aaattaaatt cattttgaat aatcatttcg ttaccttagg atcataaata ctaccattag
     4861 gaaactgggt agaccaattt ggtggtgcta gttcattttc aatttgtttt ttaattctaa
     4921 attgaatagc ttctccccat gtttgcggca tctaatgctg cttgctctgc atcaatatta
     4981 ctaaaaattg gattagtctc aaaaatgaaa aattactgtt tttttatttt ttatagcata
     5041 ttcaagtaca tgaataatat ttttcactaa gggctctgta gaaaaatctg aattttgtaa
     5101 tttttctttt acagccaaaa tactttcctt atctattagc caatccccct cacttggtcc
     5161 aaataaccaa ttatcggtaa tttcagaaac aagtctccat aatccacgat tttgatattc
     5221 aataatttga ttttccgtaa tttctacaac tgtttcagat tgaatgtttt tataagccca
     5281 atcaatcatt tcagtatatt cactaattga aacattgtct tttaaacaga aaattaacag
     5341 gttgtgttca ttcatttgtc taagttcctg atatgagttt gttgttgtaa ataatctttt
     5401 gcttgcttac cttgtaaatt aaaggattaa ccgcaccttg gatgatgccg cgaatatcat
     5461 ccatacggtt aacccaccaa tttttattgg tgtatcgtta atcatttatt tcaaaaaagt
     5521 tatttattat catattatca gggagcaggt cgttaaaaaa aaaccctcaa attccattaa
     5581 attagtattt atccagcaca taaacgaacc aaagcgttct ttccttgagt gtaaatattt
     5641 aaaattaaac tcaaaataag tttgtttgct tagaaaattt aatggttttt ctatagtgaa
     5701 aaaaaagccc ttactcgttt tagttcgaga tactatttta aataaatttt tattttcaat
     5761 aaaataagaa tattcattta ctaagttgtt atgatgaaaa agtaatttta ttaaaatgtt
     5821 ttcataattt tctaggttca tttctagctc aatttaaaat gatttcattg ttgccgtacc
     5881 aatgcgaaat ttaccatcaa caaaatttcc cttcactgat acaggtatcc catcgcagag
     5941 atgtaggtcg gatatttgta tccgacaaaa atatttgacg tgtctatcgt ttccgagaaa
     6001 acgctgttgg aaaatgtcgg attcgagaat ccgacctacg gctatatccg taaagtctga
     6061 ccattgttca atgcggaagg cgcgaatatt gcggacggaa tgtagaaacc aatatactca
     6121 attgccatgc cattcttatc aagatacctg tgctgtttaa agtgcgtggt tcattacacg
     6181 agtaatttta ttttcaggct gcctgaaaga aagataggca acttattact catcttacgc
     6241 tagccctcaa taattgaata cgttgcacta gaaccaatta tttctatgaa tgtttgctca
     6301 aacataaaaa tatagtattt aaagaattct ctattcaaag gaaaataact ttcttttaat
     6361 atccattccg cataacgctc attaagaagt tcaaaaaaag attgaccacc attttcatga
     6421 agcaaagctc tatctgcatc ttccgaaatt aaatccataa aagtatctat aaaatatgat
     6481 tcatcaacaa tcctaaaatg aataacatct tcaatagaaa tttttatggt tttatccgta
     6541 tttctataac caacggctat aacttctact ttatcaaagt ttattgctgc attatcaaaa
     6601 tagaaatagc cagttgggaa atctaagctt tttaatttca taatttatcg tgtatttcca
     6661 tatctgattt ttatttttcg gttattacta attgttattt caagtgttgg ttctccacca
     6721 ctactatcat tacgaacatt aaccgcacgc ccatcaggca aattgcccgt tatcgttcca
     6781 ttaggacgtg tttgtacatt tcgaatattt agactattaa aatcatttag tgcgtcattg
     6841 taatctccca ctttatcata ctgaacagcg ggaccttttg ttttactcct ttgagtagta
     6901 ttgctcaaaa tgtctttgat ttgtaaatca ctgcgcacct taccggcttc tatgcgagct
     6961 gccacactac tcgggctata agggtcgcta tcaatcgcag gattaccacc gctggttttt
     7021 tgtccgctag tctgtttcac aacctgctgt actgtatcgc caatttcatc taccgtacct
     7081 aacgccttag tctgcatatg gcgggcagta ggtgtttcat agccggtatt tttaacccaa
     7141 tcccatttgg tcgggttaag ttttactttt ttacctctcc aaaccgtacc tgcggcctct
     7201 gctgcggcaa gggcagtttg ggcagtagct gttatattcg gatgggcatc agcccattgt
     7261 ctggcggaat taatgccgtc ttttaccgca aaagcactgt cctgtaatag gctcgcggca
     7321 gcaagttgtg cttc
//
LOCUS       DAXHRI010000100         7265 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXHRI010000100 DAXHRI010000000
VERSION     DAXHRI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.100"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(102..2705)
                     /gene="pepN"
                     /locus_tag="ACFM75_001493"
     CDS             complement(102..2705)
                     /gene="pepN"
                     /locus_tag="ACFM75_001493"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC6256023.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYMLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSNDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPPEAELWDGT
                     ENIDPLRYHQAHEALLDTIAVRFLPKWHELNRQAAKQENQRCEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2841..3635)
                     /locus_tag="ACFM75_001494"
     CDS             complement(2841..3635)
                     /locus_tag="ACFM75_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC6256024.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGKLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4384..5253)
                     /locus_tag="ACFM75_001495"
     CDS             complement(4384..5253)
                     /locus_tag="ACFM75_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC6256025.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDIPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREEDNTVTLHFYPAWESFPSEDAQADAQRMNRFIEDRVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5304..5840)
                     /gene="ruvC"
                     /locus_tag="ACFM75_001496"
     CDS             complement(5304..5840)
                     /gene="ruvC"
                     /locus_tag="ACFM75_001496"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC6256026.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPPDAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5843..6082)
                     /locus_tag="ACFM75_001497"
     CDS             complement(5843..6082)
                     /locus_tag="ACFM75_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC6256027.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6112..7122)
                     /gene="dusB"
                     /locus_tag="ACFM75_001498"
     CDS             complement(6112..7122)
                     /gene="dusB"
                     /locus_tag="ACFM75_001498"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC6256028.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTKKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHEDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDSAGV
                     RIARKHIGWYIGEMPDGEQARREINRLDSAAAQYDMLAGYLERLAGKTDRWACGYRTN
                     AF"
BASE COUNT         1616 a         1913 c         1912 g         1824 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataagggg aagtgatgat aaaacgtgtg
       61 tgtaactata tcagacggca ttgtttttct gtttgacggc ctcaatccaa aattttgccg
      121 acgatttcgc ccacgtcttt cgacaatcct tcctgcgccc gaatgcgctg caatgcttgt
      181 ttcaccaagt ttttgcggtg cggttcgagc ttattgcaga gattgaacgc ctgcactagg
      241 cgggcggcga cctgcgggtt aaagcggtcg atttcgatga ctttgtcggc gatgaagcgg
      301 tagccgctgc cgtcttctgc gtgaaaatgc gggacgttgc ggctgaagct gccgatgagc
      361 gagcgggctt tgttggggtt ttcgaggctg aatttaggat gttgcaaggc ggtttgaacc
      421 tgttgcaggg tgtcgctgcg gcggcttgat ccaacgaggg cgaaatattt gtccatcacc
      481 agcgcgtcgt ctgaaaactt gtcggcaaac tgagccagca ggcggttgcg cgtatcgctt
      541 tcgttgccgt tgacggcgga caggatgccc cattcgtggg tcatgttttg tgccatttcg
      601 ccgtattttt cggcaacggt ttcgatgtgt gcggggtcgg cgcgcaggac gaaggcgcgg
      661 cagacattgc gcagcgtgcg ccaaccggcg gcttcggggc tgtattcgca gcgttggttt
      721 tcctgcttcg ccgcctgacg gttcaattcg tgccatttcg gcaggaagcg gacggcgatt
      781 gtatccaaca aggcttcgtg cgcctgatgg tagcgcaacg ggtcgatgtt ttccgtgccg
      841 tcccacagct cggcttcagg cggcacgccc aaaagcaggg ctttgaaggc gttgtctaag
      901 aggtcgtctg aaatgacttt ttcgacggcg gcaagcagtt tttcgtgttt cggcaacccg
      961 atgccgtctg aaagcgcggc aaggttggcg gcgacggcgc ggcggtagag cgtttgggcg
     1021 gcttcccagc gcgtgaaggc gtcgctgtcg tgggcgagca ggagcagcag gtcgtcgttg
     1081 ctgtacggat agttcagatg cactggcgcg ctgaacccgc gcagcagcga gggaacgacg
     1141 gcttcggtta cgctttcgaa ctggaaggtc tgttcggctt cggtcagcag taacacggct
     1201 tcggttgcgc gtttgccctg ataatcgaat gccaccgctt cgccgttgcg gttcagcagc
     1261 ccgactttga cgggaatcat catcggctgc ttgtccgcca tatcgggcgt gggcggcacg
     1321 gtttgtttga tggttaactc gaaactattg tttttcagac gaccttccgc ttccaagacg
     1381 ggcgtacccg cctggctgta ccacaaggcg aactggtcga gattgatgcc gttcgcgtcc
     1441 gccatcgccg cgcggaaatc gtcgcaggtc acggcctgcc cgtcgtgtcg ttggaaatag
     1501 agcttcatgc ctttttggaa gccctcttcg ccgagcaagg tgtgatacat ccgcaccact
     1561 tccgcgcctt tttcataaac ggtcatggtg tagaaattgt tcatctcctc atagctggcg
     1621 gggcgcaccg gatgggcggt cgggcctgcg tcttcgggga actggtgctg gcgcagcagg
     1681 cggatattct cgatgcggcg cacggcgcgg ctggcgcggt cgccggaaaa ttcttggtcg
     1741 cggaacacgg tcaacccttc tttcagcgaa agctggaacc aatcgcggca ggtaacgcgg
     1801 ttgcctgtcc agttgtggaa atactcgtgt ccgaccacgg attcgatgcc ttcaaaatcg
     1861 gtatcggtgg cggtacggct gtcggcaagg acgaacttgg tattaaaaat gttcagcccc
     1921 ttgttttcca tcgcgcccat attgaaatcg cccacggcga cgaccatgaa aatatccaag
     1981 tcgtattcca aaccgaagcg cgtttcgtcc cacttcattg cgtttttcag cgattccacg
     2041 gcaaagccga ccttgggctt gtccgcttcg gtggtgtaaa actcgatttt gacgtttctg
     2101 ccgctcatgg tggtgaaata gtcttccgtg accgccaaat cgcccgcgac caaagcaaac
     2161 agataactcg gtttggcaaa cgggtcttcc catttcaccc aatggcggcc gtctgaatac
     2221 tcgccgccgt cgattttgtt gccgttggag agcaaaacgg gatagcgttt tttgtccgcg
     2281 acgatggtgg tcgtgaactt ggacatgaca tccggacggt cgatatagaa cgtgattttg
     2341 cggaagccct ccggctcgca ctgggtaaac aggttaccgg cggacgcata cagccccatc
     2401 agcgatttgt tttccgccgg caggatttcg gtttccactt cgacggtgaa gcgttcggac
     2461 ggcacgtctg caatcgtcag cgtctcgcct tccaacatat aatccgccgc cgcgccgttg
     2521 attttgacgg acaagagttt cgccgaaccg tccaacacca gcggctcccc taccctctgc
     2581 ggctcaaccg tcaaacggga tttcacgacg gtttgcggtt cggcaatatc gaaatgcagg
     2641 tcggtttcga gaatacggta ggcaggcgtt tggtaatctt tcagataacg gacggttttg
     2701 ctcatttttt tctttcaata ttgtttggtt tgactggaaa aggcttcaga cggcataggc
     2761 aaaccctgca ccgatatgcc gtatgaaacc gaaacatcag tgcggacgca ccgtcaaaca
     2821 accggtttta atttcaatct ttatttccac gcttggacaa attctgccca atgcggtttg
     2881 tcttctgttt gggcggaaag atagtttctg atccgcttga tttccatttc gtattcgtcc
     2941 gcgtcgaatc tgccgctgac cagacaaaaa cgcagataca tcaaatacgt attcacggca
     3001 tcggtttcac aataattgcg gatttccttc aatttaccgg catggaatgc ctcccagacc
     3061 ttgctgccgt ccatacccaa tttacccgga aaaccgcaca gtttcgccat atcgtccagc
     3121 ggcacattcg ccctcggctg gtaaagcgcg agcaaatcca tcaaatcgca atgacgttgg
     3181 tggtagcggc tgatgtagtt gttccacttg aaatcgcggc tgtcgccgaa atcgccgtcg
     3241 cccatatccc aatagcgcgc ggcgttgatg ccgtacagca atgaacggta atgcaggaca
     3301 ggcaggtcga aaccgccgcc gttccaactg accagttgcg gcgtatgttt ttcaatcaac
     3361 tcgaaaaatt tggcaatcac gacttcttcg ccatcgtcca tctcgccgat tgtaccgaca
     3421 tggactttat cctgccccca acgcatgcag cacgaaatcg ccacaatctg atgaagatga
     3481 tgctgcataa aatccccacc cgtctgagca cggcgttttt gctgggcaaa cagcaccact
     3541 tcatcgtcgg gcagcgagga cggcagctcg tacaatgttc ggataccctg cacatcgggt
     3601 acggtttcaa tatcgaaagc caaaatcgtg gtcatgacag caccttgtat ttaaaacaga
     3661 cttgcgccta ttgtgtcatt aaaaggccga taaaaaaaga ggcaaccccc cacaggattg
     3721 ccccaatacc tcaaatcaga gatttacgct tcacaaacaa tacaggcttt cgcctgcggc
     3781 tttacccgcg tagctcaact ctacgccggc aaacttttgt ttcaccgttt ccgatgaaac
     3841 cccgaccaat cgcaagactg accggaaaat cctttcaaac agcatttcct gcctgtcgtg
     3901 taattccatg tagcgaaatg tacgccattt tctacgcttt gccaagcatt ttttacaata
     3961 taaatgtcaa aacattaatt ttataaaatt gctgaaaata ttaaatatat ggatttttat
     4021 tttttatatt tcaataaata taaatttaat tttgatttat atttaaattt aagcataaaa
     4081 tgtcaaatat taaaggaaac atgaaaggca tatattaaat atttatttat aacgctatgt
     4141 ttttaaagaa aattaatttt aatatattaa ctagattgtc tgcatatatt catgggtttg
     4201 cggtatttct tccaaaacct gcttcgaatt tcccgaccaa gtcttaaaaa tattgttttt
     4261 gagatactta aatagcagcg attatcaaat gaaatctgtt catataatct gccattttgc
     4321 atttaaaaaa acaatcagga gtttcgactc gaaatgcctg atatgttttg taattttacg
     4381 tagtcagtaa aaatcggggc tgccttccgg acgggtttta aaacgcttgt gcagccaaaa
     4441 atactgctcg ggatgttcgc gcaccctgtc ttcgataaaa cggttcatgc gctgcgcgtc
     4501 ggcttgcgca tcttcactcg gaaaggattc ccaagcaggg tagaaatgca atgtaaccgt
     4561 attgtcctcc tccctgacgg gaatggcggg tatcactttt gcatttgcaa gcgcggcaat
     4621 gcggctcaat ccggtaatcg ttgccgtctg aataccgaaa aaatccacaa aaaccgaatc
     4681 gttgcgtccg aaatcctgat cgggcagata cagaaacggc gcgctgcttt tgcggaactg
     4741 tttgacgagg gcgcgcagcc cttcggtgcg cccgataagg aagacgttgt gatagcggtt
     4801 gcggcctttc aaaatctgtt cgtccaatat cttgtttttt tgatgggaat acatactgat
     4861 cagcgggata tcctgattaa gcgcgtacac cgccatctcg aacgcggtga agtgcggata
     4921 caggatgatg actttttccc ccgccgccag cgcgtcgtcc aaataatgct tattgcggta
     4981 gcgcaccagc gatttcaaac gtccggcagg cgcgtaccaa tataaaccgt attccaacat
     5041 cagtttcgcc atgtgtttga aatgctgttt caacacggtt ttacgctttt cctcactcca
     5101 ttcggaaaaa cattttgcca aattgatttc gccgatacgg cggcgcggtt tgaccagaag
     5161 gtaggcaagc aaacccgtca ggtcggcaat cttgtgcagc agcgcaaacg gcagaaactg
     5221 caaaacatac agtacaaaaa atataaattt catctcgata cacattttct tttcagacgg
     5281 caaaatacaa atgccgtctg aaactattga aaccttccgc gcttgacctg catccctgaa
     5341 ggattgagtt tggcggcaag cccgtggttg cgtaaggcgt gggtcagcgc gacggcaaga
     5401 ccgtccgccg catccgcctg cggcgttccc gaaagcccca gcatctgcac caccatatgc
     5461 tgcacctgtt ctttcgccgc cttgcctttg ccgactaccg cctgtttgac ctgcaaggcc
     5521 gtgtattccg aaacgggcag cttatggctg accaatgccg ccaatgccgc gcccctagcc
     5581 tgacccagca tcagcgtcga tgccggattg acgttgacga acacctgttc caccgccgcc
     5641 tgctgcggct tgtaaacggc aacgacttcg ccgatgtgcc gcacaatcac ggcaatcctg
     5701 tctgccagag gcgcatcggg aggcgttttg atgcagccgg aggcgacgta aaaatgatcg
     5761 cgccccctga catcgatgat gccgaaaccc gttacgcggc tgcccgggtc gatacctaag
     5821 atacggacgg ttgccgacat attcacagca aaccgtgttg aatcagtttt ttacgcaaag
     5881 tattgcggtt cagtcccaac atgacggagg ctttggactg gttgccgccg cattgctcca
     5941 tcacgcacac cagcagcggc ttttccacct gatgcaatac catatcgtac acgccgcaag
     6001 gttcggtacc gttcaggtct ttgaaatatt gttccaaatt ttgtctgata cattgggaaa
     6061 tatcgggaag ggtatggggc atgattgcac tttcaaagga taatcaagtg ttcagaaggc
     6121 atttgtgcgg tagccgcacg cccaacggtc ggtttttccg gcaagtcttt caagataacc
     6181 tgcaagcatg tcgtattgcg ccgccgcact gtccaagcgg ttgatttcac gccgcgcctg
     6241 ttcgccgtcg ggcatttcgc cgatgtacca acctatgtgt ttgcgtgcga tgcgtacgcc
     6301 tgcactatcg ccgtaaaacg cgtgtatggc gcggatgtgg ttcaaaatag cggcggcgca
     6361 ttctgccaaa ctcaaggcag gcggtaaaac accgtgttcg gcgtaatgtt tcaaatcgcg
     6421 gaagaaccac ggtctgcctt gcgcgccgcg ccctatcatg atgccgtcgg cggcggtttg
     6481 tttgaggacg gcggcggctt tttgcggcga agtgatgtcg ccgttgaccc agaccgggat
     6541 gttcagacgg catttggttt cggcgatgag ttcgtaacgc gcctcgcctt tgtacatttg
     6601 cgtgcgcgtg cgtccgtgga cggcaagggc ggcgatgccg caatcttcgg cgattttggc
     6661 gatgacgggc aggttttgat ggtcttcgtg ccagcccaaa cgggttttga gggtaacggg
     6721 tacgcctgcc gcacggacga cggcttccaa aatggcggca accagcggct cgttctgcat
     6781 cagcgcgctg ccggcttgga cgttgcacac ttttttggcg ggacagccca tattgatgtc
     6841 gataagctgc gccccgaggc tgacgttgta acgcgccgca tccgccatct gctgcggatc
     6901 gctgccggca atctgcacgg caacgatgcc gccttcatcg gcaaaatcgc tgcggtgcag
     6961 ggtctttttg gtatttctaa gtgtcgggtc gctggtcagc atttcgcaca ccgcccaacc
     7021 cgcgccgaaa tcgcggcaga ggcggcggaa gggtttgtcg gtaatgcccg ccatcggtgc
     7081 aagtgcgatg gggttgtcga taaaatagcc gccgatgtgc ataatggatc cgcgtttcaa
     7141 aaaagtgcgc cattgtacat tttttaagca ggatttccaa tctccggacg cgcccgcgat
     7201 tggggcggac accgttttat ggcataatcc gcacacagat tccctgcccc gccactcaca
     7261 ggcgg
//
LOCUS       DAXHRI010000101         7216 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXHRI010000101 DAXHRI010000000
VERSION     DAXHRI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.101"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(67..426)
                     /gene="rnpB"
                     /locus_tag="ACFM75_001499"
     ncRNA           complement(67..426)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFM75_001499"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(505..1608)
                     /gene="prfB"
                     /locus_tag="ACFM75_001500"
     CDS             complement(505..1608)
                     /gene="prfB"
                     /locus_tag="ACFM75_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC6256029.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1711..2604)
                     /locus_tag="ACFM75_001501"
     CDS             complement(1711..2604)
                     /locus_tag="ACFM75_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC6256030.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2946..3395
                     /locus_tag="ACFM75_001502"
     CDS             2946..3395
                     /locus_tag="ACFM75_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6256031.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3469..3777
                     /locus_tag="ACFM75_001503"
     CDS             3469..3777
                     /locus_tag="ACFM75_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256032.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3893..4735
                     /locus_tag="ACFM75_001504"
     CDS             3893..4735
                     /locus_tag="ACFM75_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC6256033.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAERVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            4725..5471
                     /locus_tag="ACFM75_001505"
     CDS             4725..5471
                     /locus_tag="ACFM75_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC6256034.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDSFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5493..6080
                     /locus_tag="ACFM75_001506"
     CDS             5493..6080
                     /locus_tag="ACFM75_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC6256035.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDNFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6086..6265
                     /locus_tag="ACFM75_001507"
     CDS             6086..6265
                     /locus_tag="ACFM75_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC6256036.1"
                     /translation="MKKCRDITLLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6497..6937
                     /locus_tag="ACFM75_001508"
     CDS             6497..6937
                     /locus_tag="ACFM75_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6256037.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1855 a         1916 c         1622 g         1823 t
ORIGIN      
        1 acaacctaaa taaaaatgcc gtctgaacca tatttcaggt ttcagacggt atttgcgtgt
       61 cggatgcaca ccggacaggc ggtaagccgg gttctgtctc ggacagtcat tcctctaggc
      121 ataccgttgc cagtatgctc aagcaaccta cccgaacgct cggcgggcag cgtcattgcg
      181 ttctgtttgg tcttgctccg aatggggttt ggcctgccgc atattgttac caaatgcgcg
      241 gtgcgccctt accgcacctt ttcacccttg cctgtgctgc caaagcagcc atcggcggtt
      301 ttgctttctg ttccactttc cgtcgcgtta ccgcgcccgg ccgttaaccg gcattctacc
      361 ctgcggagcc cggactttcc tccccgtatg ccttacgcga tacgcggcga ctgtctgccc
      421 atcccgtgtg cggcgcggat tataacacga aacgcaaaaa taccgtctga aacggtacag
      481 gtttcagacg gcatacggtt caaactacac gccctgtttc aggctggctt cgataaagcc
      541 gtccaaatcg ccatccaata cggctttggt gttgccgact tcgtagcctg tacgcaagtc
      601 tttgatacgt gaggaatcca aaacatacga acggatttgg ctgccccaac ctacatcgga
      661 tttaccttct tccaacgcct gtttctcttc gttgcgtttg cgcatttcca attcatacag
      721 tttggatttc aacatttcca tcgcggccgc tttgttggcg tgttgcgaac ggtcgttttg
      781 acattgcacc acaatccccg tcggctcgtg ggtaatacgc acggcggagt cggttttatt
      841 gatgtgctga ccgcccgcgc ccgatgcgcg ataggtgtcg atacgcaaat cggcggggtt
      901 gatttcgatt tcaatggaat catcgatttc agggtaaacg aacacggagg caaacgaagt
      961 atggcgtttg ttgttcgagt caaacggtga atagcgcacc aagcggtgaa cgccggtttc
     1021 ggtacgcagc aaaccatagg catattcgcc ttccactcgg atagtcgcgc ggttgatgcc
     1081 cgcgatttcg ccgtcgtctt cttcgaggat ttcgattttg aagcctttgc gctcggcgta
     1141 gcggctgtac atacggaaca gcatacccgc ccagtcttcc gcttccgtac cgcctgcgcc
     1201 tgcggtaatg tcgataaagc agttgttcgg atcggcgggc tggttgaaca tccgtttgaa
     1261 ctccaaatcc gccatctgtt tttccagccc cgccacatct tcctgcacgg cggcaaaacc
     1321 ttcttcgtcg ttttcttcga cggtcatttc aatcagcatg cggttgtctt cgatgcccga
     1381 agcgatgttg tcgagcgtca acacgatgcc ttcgaggatt ttgcgctctt tgccgatttc
     1441 ttgggcgcgt ttcgggtcgt tccaaagttc ggggtcttcg gaaagaccga caacttcttc
     1501 caatcggtct ttcttaccct gataatccat ataaactcgg atgtcttcgc tgcgcttttc
     1561 caaatcattc agggtattgt tgagctggtt gattacttcg gcttccatga ttcttttgtt
     1621 ctttcaaaat tttaggggcg tattgtacgg gattcgggta tttttttcta tggataaagc
     1681 cttctggaaa cacgttcaga cggcatagcg tcaataacgg tatgccgcca gtttgcgttt
     1741 gatttcaggc aatgcggcac gcgctgcctc ctcgcccaac cgaatggcgc gttttttctg
     1801 atcgaaaccg ccgactgcac ccaaatccaa aacctgcggt ttgataacca catccgcctg
     1861 ccccaactca ttttgcaacg cagaaacgct cattacgttc agcgtctgat cgagataaga
     1921 gaagaaacct tggctgatgt ttttgcccgg acgggcggaa atatcgacgg caatcacgaa
     1981 attcgccccc tgccgccggg cggcactgac gggcacgggc tgcgacagac cgccgtcaac
     2041 atatgtatgc ctgccgatga taacgggttg gaacacattg ggaatggcgg cggaagcgcg
     2101 cacagcctgc ccggcattcc cttgattgaa agcgacggcc ttgccggttt caaaatcagt
     2161 agcaacagcg gcaaatttga tgggaaactg ctgaatccgc ctgccgccga cttttcggtt
     2221 gatgtaattt tgcagctttt cgcctttgat aaaaccactg gtggacaagg ttaaatcgac
     2281 taaatcggtt ttgcctaaaa tttcggcttc caattcgagg cggtcgggcg acatacccga
     2341 tgcaaaaagg ctgccgacaa tcgaacctgc cgatgtaccg gtaaccacct tcacaggaat
     2401 accgttttct ttcaaaacct taataatacc tacatgggca aatcctttag atgcgccgcc
     2461 accgagtgcc aaaccgacca ctgcggcggg tttggcggtt tgcaccggct tgcgggcggc
     2521 attatttccc gccgtgccgc aggcggcaag caacgcggcg gcggcgattg ccaaaagcgg
     2581 tctgattttt gaaaacgtta ccatattttc cattccttta tatatcgcac cccgtcaaaa
     2641 agagggattg cttttcttaa caaccccctt tgacagccaa gcaaatgggg gctttgttag
     2701 gtcatcatca aaattaatat ttcttttttt cctttacgga aattatattt gaaggcatac
     2761 tatccaaggt gggaattatc tcacaacacc gccgttatcc aaatatcccg cctttttccc
     2821 ttcctttcca tcaaaatact ttctttttat attcattaac ttgttaaatc attggctgcc
     2881 gggtgtcagt ttttccgaca aaatccgtct aatggggtat caacagaacc aaaacaggaa
     2941 cacttatgaa aatcggaaca acttggcaga cggcatccgc tatgctggtt ttgcgtctgt
     3001 ttgccgcata tgaatttttg gaatcgggtt tgcaaaaatg gaacggggag aattggtttt
     3061 ctgaaatcaa cgatcaattc ccattcccgt tcaacttgct gccggacgcg ttaaactgga
     3121 atctcgctat gtatgcggag cttttactgc ccgtactgtt gcttttgggt ttggcaacgc
     3181 gtctgtcggc attagggctg atggtcgtta ccgccgtcgc ttgggctgcg gttcacgccg
     3241 gttcgggtta caatgtctgc gacaacggtt ataaaatggc tttaatttat atcgtggtat
     3301 taatcccgct gcttttccag ggtgcgggcg gatggtcgct ggatacgctg ctgaaaaaac
     3361 ggttttgccc ccaatgccgt ctgaagcaag attgattcag tcgtggaatc tgactttaaa
     3421 cattccaacc ttatctcgtt aacttgatat tctgaaaagg aaatagaaat gaacaaaaac
     3481 attgctgccg cactcgccgg tgctttatcc ctgtctttgg ccgccggtgc agttgctgcc
     3541 aacaaaccgg caagcaacgc aacaggcgtt cataaatccg cccatggctc ttgcggcgcg
     3601 tccaaatctg ccgaaggttc gtgcggcgcg gctggttcta aagcaggcga aggcaaatgc
     3661 ggcgagggca aatgcggtgc gaccgtaaaa aaaacccaca aacacaccaa agcatctaaa
     3721 gccaaggcca aatctgccga aggcaaatgc ggcgaaggca aatgcggttc taaataatcc
     3781 caccccttca aaccaagccg cgtttttcag taaaatgcgg cttttttaac ggcaaacaaa
     3841 gattttttaa caagcacatc attcttttgt gccatccgaa ccgggtaaaa atatgattca
     3901 acacgcaggc ttgggctacc gccgcgactt ggcggaagac tttctctcgc tttccgaaaa
     3961 cagcccgata tgctttatcg aagccgcacc ggaaaactgg ctgaaaatgg gcggctgggc
     4021 acgcaaacag tttgaccgtg tggcggaacg gctgccgctg gcgttgcacg gattgtctat
     4081 gtcgctgggc gggcaagcac cgctggatac tgatttgata gacggcatca aagaaatgat
     4141 gcgccgttac gattgcacgt ttttctccga ccatttgagc tactgccacg acggcggtca
     4201 tctttacgat ttgttgccgc tgccctttac cgaggaaatg gtgcatcata cggcgcggcg
     4261 tatccgcgaa gtgcaagacc gtttgggctg ccgcatcgcc gtggaaaaca cgtcctacta
     4321 tctgcattcc ccgcttgccg agatgaacga ggtcgagttc ctcaacgccg tcgcacgtga
     4381 ggccgattgc ggcattcatc tggatgtgaa caatatctac gtcaacgccg tcaatcacgg
     4441 tctgctgtcg ccggaggctt ttttggaaaa tgtggatgca gagcgcgtgt gctatatcca
     4501 tattgccgga catgacgtgg aaacgccgga attgttgatt gatacacatg gcgcggcagt
     4561 tttgccgact gtttgggact tgctcgaact tgcctatgcc aagctgccga cgattccgcc
     4621 caccctgttg gaacgcgatt ttaatttccc gccttttgcc gaactcgaag ccgaagtcgc
     4681 caaaatcgcc gattatcaaa cgcgtgccgg aaaggaatgc cgccgtgcag cctgaaacct
     4741 ccgcccaata ccagcaccgt ttcgcccaag ccatacgcgg gggcgaagcc gcagacggtc
     4801 tgccgcaaga ccgactgaac gtctatatcc gcctgatacg caacaatatc cacagcttta
     4861 tcgaccgctg ctataccgaa acgcggcaat actttgacag cgaagaatgg agccgtctga
     4921 aagaaggttt cgtccgcgac gcgcacgccc aaacgcccta ttttcaagaa atccccggcg
     4981 agttcctaca atattgccaa agcctgccgc tttcagacgg cattttggca ctgatggatt
     5041 ttgaatatac ccaattgctg gcagaagttg ctcaaattcc ggatattccc gacattcatt
     5101 attcaaatga cagcaaatac acaccttccc ctgcggcctt tatccggcaa tatcgatatg
     5161 atgttaccga tgatttgcat gaagcggaaa cagccttgtt aatatggcga aacgccgaag
     5221 atgatgtgat gtaccaaaca ttggacagct tcgatatgat gctgctagaa ataatgggtt
     5281 cctccgcgct ttcgtttgac accctcgccc aaacccttgt cgaatttatg cctgaggacg
     5341 ataattggaa aaatattttg cttgggaaat ggtcaggctg gactgaacaa aggattatca
     5401 tcccctcctt gtccgctata tccgaaaata tggaaggcaa ttccccgggc caaaaccatc
     5461 tatccgcata aaattacctt gttcccgata ctatgccgct acccgacctg accgatgccg
     5521 aattaataga gtcgcgtaaa ctgcttctgc attttgcgcg gcttcaattg cccgaccacc
     5581 ctgatttggc tgaagattta gtgcaggaaa cattgctgtc cgcatacagc gcaggcgaca
     5641 attttcaagg cagggcactt gtcaacagct ggctttttgc catattgaaa aacaaaatta
     5701 ttgacgcatt acgtcaaatc ggaaggcaga ggaaagtctt taccacactg gatgacgagc
     5761 tgctggatga agcatttgaa agccattttt cccaaaacgg gcattggacg ccggaagggc
     5821 agccgcaaca ttggaacact ccggaaaaat cattaaacaa caacgaattc caaaaaattc
     5881 tgcaaagctg cctatacaac ttgcctgaaa acaccgcacg ggtatttacc ctgaaggaaa
     5941 tactcggttt ttcatccgac gaaatacaac aaatgtgcgg tatcagcacg tccaactacc
     6001 acaccattat gcaccgcgcc cgagaatcat tgcgccaatg cctgcaaatc aaatggttca
     6061 accaagaaaa cccgaagtaa acgttatgaa aaaatgccgc gatatcaccc tgcttctttc
     6121 caaacatcag gaccgggaaa ccaccccggg cgagaagatt tccatataca cacacctgct
     6181 gttctgtccg tattgccgtg aatataaaag acaacttcaa accatcaaaa gatcactggc
     6241 aaaaacaacc agaacttcaa aataaatgcc gtctgaaaag gcttcagacg gcataagctg
     6301 acggaaacaa atcaaaccga tttactgtta tcggcagttc atccataata cacgcttcaa
     6361 aagcagcata ttcctccata cggaatatat aaatacgcaa aacacgaagg ctgcatcaat
     6421 ttgctttatt tgccttattt cacagacggc gctacccctc ccgcccaacc cgttctttct
     6481 gaatgagcag atttcaatga ttaaggaaac cctaatgcgc ccaatcttcc tatctttcgt
     6541 tttactccct attttgataa ccgcctgcag cacaccggac aagtctgccc gatgggaaaa
     6601 tatcggcaca atctcaaacg gcaatattca tacatatatc aataaagaca gcgtgagaaa
     6661 aaacggaaat ctgatgattt tccaagataa aaaagttgtt accaatctaa aacaagaacg
     6721 ttttgccaac acccccgcat acaagactgc cattgccgag tgggaaatcc actgcaacaa
     6781 caaaacatac cgcttaagtt cgctacagtt gtttgataca aaaaacacgg aaatttccac
     6841 acaaaactac acagcctctt ccctccgccc gatgagcatc ctgtccggga cattaaccga
     6901 aaaacaatat gaaaccgtat gcgggaaaaa actctgatta caacttatac acaaacttat
     6961 ccataagcct tatcataaaa tgccgtctga aatactgaaa tatcagcatt tcagacggca
     7021 ttttaccatt tctccgaaaa gttatcctca aagttatcca cattattttt ttaaaccagc
     7081 ttccatccga aatataaacc tgctatacaa tttcattatc aaaacgtaaa ttgttccatt
     7141 gatacacaaa actgcttacc cccataattt tgataaagca tttcttacat tcccggctcc
     7201 gtcccgtaac caacac
//
LOCUS       DAXHRI010000102         7090 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXHRI010000102 DAXHRI010000000
VERSION     DAXHRI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.102"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="ACFM75_001509"
     CDS             complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="ACFM75_001509"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC6256038.1"
                     /translation="MSDNVPTIAAVATASGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1553..1708
                     /locus_tag="ACFM75_001510"
     CDS             1553..1708
                     /locus_tag="ACFM75_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256039.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1705..2136
                     /locus_tag="ACFM75_001511"
     CDS             1705..2136
                     /locus_tag="ACFM75_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC6256040.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2270..6622
                     /gene="app"
                     /locus_tag="ACFM75_001512"
     CDS             2270..6622
                     /gene="app"
                     /locus_tag="ACFM75_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC6256041.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGQQWQRTDEQQAKGDAYSS
                     WLAGAYNWRIAGNTHSQSGAGNGTVNLSGDITKSNNYGPLPTAGSFGDSGSPMFIYDA
                     IKQKWLINGVLQTGNPYLGAGNGFQLIRKDWFYGNVFAEDLPITFLEPRSNGHYSFSS
                     NNNGTGTVTQTNEKVSMPRFKVRTVQLFNEALKAEDKEPVYAAGGVNAYKPRLNNGKN
                     ISFIDYAKGEVTFTNNINQGAGGLYFEGDFTVSSENNATWQGAGVHISEDSTVTWKVN
                     GVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVSGR
                     GTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKESTVT
                     ITGNKDITTTGNNNSLDSKKEIAYNGWFGEKDSTKTNGRLNLVYQPAAEDRTLLLSGG
                     TNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWIDRTFKAEN
                     FLIKGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTI
                     TDEKVIASLTNTDISGNVSLANHAHLNLTGLATLNGNLTAGGDTHYEVTHNATQNGNL
                     SLVGNAQATFNQATLNGNTSASGNASFNLSDHAAQNGDLTLSGNAKANVSHSAFNGNV
                     SLADKAVFHFENSRLTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAY
                     RHDAAGAQTGSATDAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMSEL
                     FGYRSDKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEH
                     VDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAGRD
                     AVEKTESVAEPARQGGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRA
                     RRARRDLPQPQPQPQPQRDLISRYTNSGLSEFSATLNSVFAVQDELDRVFAEDRRNAV
                     WTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGN
                     SARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDDIGSKIRRRVLHYGIQARYRAGF
                     GGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITP
                     YLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQL
                     EAQHSAGIKLGYRW"
     gene            complement(6731..>7090)
                     /locus_tag="ACFM75_001513"
     CDS             complement(6731..>7090)
                     /locus_tag="ACFM75_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256042.1"
                     /translation="NPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVFWDGNK
                     RTATLFANGLMMERGRGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNCISGID
                     YFGADEDIDFPDPPNMG"
BASE COUNT         1903 a         2011 c         1731 g         1445 t
ORIGIN      
        1 cgaacggatc ggattcccgt ttttatggga taacggaatg ttcagccgga caccgccacg
       61 atgctttcga tccgtttatt ttccgataca aaacctcgaa aaaatcacgc cgagcaggtc
      121 gtccgccgta aactcgcccg tgatttcgcc gcatgcgacc tgcgccaagc gcaagtgttc
      181 ggcaaacagc tcgatttgat ggttgccgca caatgccgcc agcgacaatt cttcctgcgc
      241 tgctttgagt gcgttgacgt gccgcgtccg cgccaaaaac agcccttcgc tttcgccctg
      301 ccaaccggcc tcacgcaaca acgtccgttt cagcgcgtcc aagccgtcgc cggttttcgc
      361 cgacagcgcg atgacggttt ccgcgcccgt accgaacccg cctgccgcgt gtgcgtgcaa
      421 atcggatttg ctgtggattt cgatgcgttt caactccggc ggcaacgcgt ccaaaatcgc
      481 ccgtgtcttt tcattcaaac cctcgcgcgg atcgaccaac accagcgcga catcggcttc
      541 ggatacggct ttgcggctgc gttcgatgcc gatacgctcg accacgtcgt ccgtctcgcg
      601 caaacctgcc gtatcgacaa tatgcaccgg cacgccgtca atcaggatac gttccctgac
      661 cgcgtcgcgc gtcgttccgg caatatcggt aacaatcgcc acttcgtcgc ccgccaacgc
      721 gttcagcagg ctggacttgc ccacattcgg cgcgccgacc aatacgacat tcagaccttc
      781 gcgcaaaatc gcgccctgct gcgcgttggc aagcacatca tccacggcgc ggcgcaagcc
      841 gtccagtttg ccgcgtgcgt ctgccgcttc gagaaaatcg atgtcttcct cgggaaaatc
      901 cagcgtcgct tcgaccagca tccgtaaggt aatcaagtct tcgaccagac cgtgtatccg
      961 ccgcgaaaaa tcgcccttga gcgagcgcaa cgccagacgc gccgccgaac ggctggatgc
     1021 gtcaatcaaa tccgccacgc cttccgcctg tgccaagtcc agtttgtcgt ttaaaaacgc
     1081 acgtttggta aactcgcccg gttcggcaag gcgcgcgccc aactccaaac agcggttcag
     1141 cagcatatcc atcaccaccg gcccgccgtg tccctgaagc tcgatgacat cttcacccgt
     1201 aaaacttgcc ggtgcggcaa aaaacagcaa aagcccgctg tcgattgcct gaccgtccgc
     1261 gtccgtaaaa tcagcatagg ttgccacgcg cggcttgggc gttttcccac acaaagcctc
     1321 tgccatcggc agcaggtttt tccccgatat gcgtatcacg cccacgccgc cgcgccctga
     1381 tgcggtagcg actgccgcaa tcgttggaac gttatccgac ataaaacccc cgaaaattca
     1441 aaacagccgc gattatagca aatgccgtct gaagtccgac ggtttggctt tcagacggca
     1501 taaaaccgca aaaacgcttg ataaatccgt ccgcctgacc taatataacc atatggaaaa
     1561 acgaaacaca tacgccctcc tgctcggtat aggctcgctg ctgggtctgt tccatcccgc
     1621 aaaaaccgcc atccgcccca atcccgccga cgatctcaaa aacatcggcg gcgattttca
     1681 acgcgccata gagaaagcgc gtaaatgacc gaaaacgcac aggacaaggc gcggcaggct
     1741 gtcgaaaccg tcgtcaaatc cccggagctt gtcgagcaaa tcctgtccga cgagtacgtg
     1801 caaataatga tagcccggcg tttccattcg ggaccgttgc cgccgccgtc cgacttggcg
     1861 caatacaacg acattatcag caacggggca gaccgcatta tggcaatggc ggaaaaagaa
     1921 caagccgtcc ggcacgaaac catacggcaa gaccaaacct tcaacaggcg cgggcaactg
     1981 tacggcttca tcagcgtcat cctgatactg ctttttgccg tcttcctcgt atggagcggc
     2041 taccccgcaa ccgccgcctc ccttgccggc ggcacagtgg ttgccttggc gggtgctttc
     2101 gtgattggaa gaagccgaga ccaaggcaaa aattaatgac aaatcctagg gcgtgcttca
     2161 tatccgcccg aacgccgaac cgcacatata ggcacatccc gcgcgccgcc cggaagcgga
     2221 agccgcgccc tcccaaacaa acccgaatcc cgtcagataa ggaaaaataa tgaaaacaac
     2281 cgacaaacgg acaaccgaaa cacaccgcaa agccccgaaa accggccgca tccgcttctc
     2341 gcctgcttac ttagccatat gcctgtcgtt cggcattctt ccccaagcct gggcgggaca
     2401 cacttatttc ggcatcaact accaatacta tcgcgacttt gccgaaaata aaggcaagtt
     2461 tgcagtcggg gcgaaagata ttgaggttta caacaaaaaa ggggagttgg tcggcaaatc
     2521 aatgacaaaa gccccgatga ttgatttttc tgtggtgtcg cgtaacggcg tggcggcatt
     2581 ggtgggcgat caatatattg tgagcgtggc acataacggc ggctataaca acgttgattt
     2641 tggtgcggag ggaagcaatc ccgatcagca ccgcttttct taccaaattg tgaaaagaaa
     2701 taattataaa gcagggacta acggtcatcc ttatggcggc gattatcata tgccgcgttt
     2761 gcataaattt gtcacagatg cagaacctgt tgaaatgacg agtgacatga gggggaatac
     2821 ctattccgat aaagaaaaat atcccgagcg tgtccgcatc ggctctggac aacaatggca
     2881 aagaacggat gaacaacaag cgaaggggga cgcttatagc tcatggcttg ctggcgctta
     2941 taattggaga atagcaggta acacacattc acaaagtgga gcgggcaacg gcacggtaaa
     3001 cttaagtgga gatatcacaa aatctaataa ttatggacct ttaccaactg cgggatcttt
     3061 cggcgatagt ggatcgccaa tgtttattta tgatgcaata aaacaaaaat ggcttattaa
     3121 tggcgtattg caaactggta acccttattt gggggcggga aatggattcc aattaattag
     3181 aaaagattgg ttctatggca atgtctttgc agaagatttg cctataacat ttttagaacc
     3241 aagaagtaac ggtcattatt cattttcttc aaataataat ggaactggta cggttactca
     3301 aacgaatgaa aaagtgagta tgcctcgatt taaagtcaga acggttcagt tatttaatga
     3361 agcattaaaa gcagaagata aagagcctgt ttatgctgca ggtggtgtaa atgcttataa
     3421 accaagacta aataatggta aaaatatttc atttattgac tatgcaaaag gcgaagtcac
     3481 ttttactaat aatattaatc aaggtgctgg tggtttgtat tttgaaggtg actttacggt
     3541 atcttcagaa aataatgcaa cttggcaagg cgcgggcgtt catatcagtg aagacagtac
     3601 cgttacttgg aaagtaaacg gcgtggcaaa cgaccgcctg tccaaaatcg gcaaaggcac
     3661 gctgcacgtt caagccaaag gggaaaacca aggctcgatc agcgtgggcg acggtaaagt
     3721 tattttagat caacaagcag atgaaaataa taaaaaacaa gcctttagtg aaatcggctt
     3781 ggtcagcggc agggggacgg tgcaactgaa tgccgataat cagttcaacc ccgacaaact
     3841 ctatttcggc tttcgcggcg gtcgtttgga tttgaacggg cattcgcttt cgttccaccg
     3901 tattcaaaat accgatgaag gggcgatgat tgtcaaccac aatcaagaca aagaatccac
     3961 cgttaccatt acaggcaata aagatattac tacaaccggc aataacaaca gcttggatag
     4021 caaaaaagaa attgcctaca acggttggtt tggagagaaa gattcaacca aaacgaacgg
     4081 tcggctcaac cttgtttacc agcccgccgc agaagaccgc accctgctgc tttccggcgg
     4141 aacaaattta aacggcaaca tcacccaaac aaacggcaaa ctgtttttca gcggcagacc
     4201 gacaccgcac gcctacaatc atttaaacga ccattggtcg caaaaagagg gcattcctcg
     4261 cggggaaatc gtgtgggaca acgactggat cgaccgcaca tttaaagcgg aaaatttcct
     4321 gattaaaggc ggacaggcgg tggtttcccg caatgttgcc aaagtagaag gcgattggca
     4381 tttgagcaat cacgcccaag cagttttcgg tgtcgcaccg catcaaagcc acacaatctg
     4441 tacacgttcg gactggacgg gtctgacaaa ttgtgtcgaa aaaaccatta ccgacgaaaa
     4501 agtgattgct tcattgacca ataccgacat cagcggcaat gtcagccttg ccaatcacgc
     4561 tcatttaaat ctcacagggc ttgccacact caacggcaat ctgaccgcag gcggagacac
     4621 gcactatgag gttacgcaca acgccaccca aaacggcaac cttagcctcg tgggcaatgc
     4681 ccaagcaaca tttaatcaag ccacattaaa cggcaacaca tcggcttcgg gcaatgcttc
     4741 atttaatcta agcgaccacg ccgcacaaaa cggcgatctg acgctttccg gcaacgctaa
     4801 ggcaaacgta agccattccg cattcaacgg caatgtctcc ctagccgata aggcagtatt
     4861 ccattttgaa aacagccgcc ttaccggaca aatcagcggc ggcaaggata cggcattaca
     4921 cttaaaagac agcgaatgga cgctgccgtc aggcacggaa ttaggcaatt taaaccttga
     4981 caacgccacc attacactca attccgccta tcgccacgat gcggcagggg cgcaaaccgg
     5041 cagtgcgaca gatgcgccgc gccgccgttc gcgccgttcc ctattatccg ttacaccgcc
     5101 aacttcggca gaatcccgtt tcaacacgct gacggtaaac ggcaaattga acggtcaggg
     5161 aacattccgc tttatgtcgg aactcttcgg ctaccgcagc gacaaattga agctggcgga
     5221 aagttccgaa ggcacttaca ccttggcggt caacaatacc ggcaacgaac ccgtaagcct
     5281 cgatcaattg acggtagtgg aagggaaaga caacaaaccg ctgtccgaaa accttaattt
     5341 caccctgcaa aacgaacacg tcgatgccgg cgcgtggcgt taccaactca tccgcaaaga
     5401 cggcgagttc cgcctgcata atccggtcaa agaacaagag ctttccgaca aacttggcaa
     5461 ggcagaagcc aaaaaacagg cgggaaaaga caacgcgcaa agccttgacg cgctgattgc
     5521 ggccgggcgc gatgccgtcg aaaagacaga aagcgttgcc gaaccggccc ggcagggagg
     5581 cggggaaaat gtcggcatta tgcaggcgga ggaagagaaa aaacgggtgc aggcggataa
     5641 agacagcgcc ttggcgaaac agcgcgaagc ggaaacccgg ccggctacca ccgccttccc
     5701 ccgcgcccgc cgcgcccgcc gggatttgcc gcaaccccaa ccgcaacccc aaccgcagcg
     5761 cgacctgatc agccgttata ccaatagcgg tttgagtgaa ttttctgcca cgctcaacag
     5821 cgttttcgcc gtacaggacg aattagaccg cgtatttgcc gaagaccgcc gcaacgccgt
     5881 ttggacaagc ggcatccggg acaccaaaca ctaccgttcg caagatttcc gcgcctaccg
     5941 ccaacaaacc gacctgcgcc aaatcggtat gcagaaaaac ctcggcagcg ggcgcgtcgg
     6001 catcctgttt tcgcacaacc ggaccgaaaa caccttcgac gacggcatcg gcaactcggc
     6061 acggcttgcc cacggcgccg ttttcgggca atacggcatc ggcaggttcg acatcggcat
     6121 cagcacgggc gcgggtttta gcagcggcag tctttcagac gacatcggaa gcaaaatccg
     6181 ccgccgcgtg ctgcattacg gcattcaggc acgataccgc gccggtttcg gcggattcgg
     6241 catcgaaccg cacatcggcg caacgcgcta tttcgtccaa aaagcggatt accgctacga
     6301 aaacgtcaat atcgccaccc ccggccttgc gttcaaccgc taccgcgcgg gcattaaggc
     6361 agattattca ttcaaaccgg cgcaacacat ttccatcacg ccttatttga gcctgtccta
     6421 taccgatgcc gcttcgggca aagtccgaac gcgtgtcaat accgccgtat tggctcagga
     6481 tttcggcaaa acccgcagtg cggaatgggg cgtaaacgcc gaaatcaaag gtttcacgct
     6541 gtccctccac gctgccgccg ccaaaggccc gcaactggaa gcgcaacaca gcgcgggcat
     6601 caaattaggc taccgctggt aacaccggat atgccgaaag gggtttgacg atgccgccgt
     6661 gcgctgtcaa accctttttc tgccgccgcc ttgtgcctgc tccattgtct gataaccgtc
     6721 aaattgattt ttagcccatg tttggtgggt cggggaaatc tatatcttcg tccgcgccga
     6781 aatagtctat gcccgatata caattttgat acacaaactt ggaaatatcg gtatcgtcac
     6841 ccgagcgata gaatgcggac agtttttcat tgaattgcgg catctgcatt tcggagattt
     6901 ccaagatgcc acggcccctc tccatcatca gaccattggc aaataaggtt gccgttcgtt
     6961 tgttgccgtc ccaaaaaacc tgctgccgca tacaataaag cataaaacgg acggctgcct
     7021 ccgtcgtcga accgctttgc agtccgatat tttgtaacac gcgagccact tcaatttcct
     7081 tcaccggatt
//
LOCUS       DAXHRI010000103         7060 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXHRI010000103 DAXHRI010000000
VERSION     DAXHRI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.103"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            147..728
                     /locus_tag="ACFM75_001514"
     CDS             147..728
                     /locus_tag="ACFM75_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6256043.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(789..1643)
                     /gene="folD"
                     /locus_tag="ACFM75_001515"
     CDS             complement(789..1643)
                     /gene="folD"
                     /locus_tag="ACFM75_001515"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC6256044.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHTPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAIVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1866..1943
                     /locus_tag="ACFM75_001516"
     tRNA            1866..1943
                     /locus_tag="ACFM75_001516"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1901..1903,aa:Pro,seq:tgg)
     gene            1968..2044
                     /locus_tag="ACFM75_001517"
     tRNA            1968..2044
                     /locus_tag="ACFM75_001517"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2002..2004,aa:Arg,seq:tct)
     gene            2072..2147
                     /locus_tag="ACFM75_001518"
     tRNA            2072..2147
                     /locus_tag="ACFM75_001518"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2105..2107,aa:His,seq:gtg)
     gene            2283..2789
                     /gene="rfaE2"
                     /locus_tag="ACFM75_001519"
     CDS             2283..2789
                     /gene="rfaE2"
                     /locus_tag="ACFM75_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC6256045.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGDALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2786..4564
                     /locus_tag="ACFM75_001520"
     CDS             2786..4564
                     /locus_tag="ACFM75_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC6256046.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPLVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4677..5501
                     /locus_tag="ACFM75_001521"
     CDS             4677..5501
                     /locus_tag="ACFM75_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC6256047.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6264..7052)
                     /locus_tag="ACFM75_001522"
     CDS             complement(6264..7052)
                     /locus_tag="ACFM75_001522"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC6256048.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         1499 a         1834 c         2124 g         1603 t
ORIGIN      
        1 taaaattatg tatggcgtaa aggaagcgtt ttaacgccgt attgggaaaa tgtaaagttc
       61 tttatcggga tttctttaag tattggcgtt ttcggtgtcg tggcggtaga atcggggtca
      121 gttaagaaca attatcagga acggatatgg aagaaaaaaa cgactacacc gacgcggcat
      181 ccgacaaccg caacgggcag gaaattgaag tcggaattgc cggcgcaggc gaccggatac
      241 ttgccgcgct gctgaaccaa ttgtttacct ttttggtttt gttggtgccg tttgccggac
      301 tgattgcttt tgccatcaag aatgaaggca ggattggcgg cagtgaggaa atattcgggc
      361 tgcttttggg catgacttcc ttttgggtag gactggcggg cattttggtt tataccgtcg
      421 ttcaaatcta ctatatgagt cgggacgggc agtcattggg taagaaaatc atgagaatcc
      481 gcgtgttgaa aaccgacggc cgcaatcccg gttttgtcgg cacggttttg gtacgcgaaa
      541 tcgcatggtc ggttttggtt gccattattg ccgccgttat cggtcttgca gtaggtgaca
      601 acggagaaaa cgccatcaac ctgctggcat tccttgccaa ctttgtcctg ctctttatgg
      661 tcaaacgcga ccgccgcacg ctttacgaca tactggcgga tacggttgtc gtcaagctgc
      721 ccaaataaac ggacacggca aaatgccgct gaaagccttt cagacggcat ttttatcttc
      781 agaaccgctc aagcatcgtg cagtgaagcc gcgtgcaggg tgttttccat caatgtggca
      841 atcgtcatcg gacccacgcc gccgggaacg ggcgtaatca tcgccgcccg ttcttttgcc
      901 gtttcaaatt ccacgtcgcc gcacaggctg ccgtcgtcca aacggttgat gcccacatcg
      961 ataacgaccg cgccgggttt gatccattcg cctttgacaa agttcggaat gcccacgccg
     1021 accaccaaaa tatcggctcc ggcaacctcg tctgccagat tttcggttgc gctgtggcag
     1081 accgttaccg ttgcgcgtgc cagcagcaat tccaacgcct gcgggcggcc gacgatattc
     1141 gacgcgccga ccacgatcgc ttttttcccc ttcggatcaa tgccgtaagc ttccaaaagc
     1201 gtcatcacgc ccttgggcgt acacgggcgc atcagcggca ttttgaccgc cagcctgccg
     1261 acattgtaag gatggaagcc gtccacgtcc ttatccggcg aaatacgttc caaaaccgcc
     1321 tggctgtcga ggtgcttcgg cagcggtagc tgaaccagaa taccgtccac ttcggaatcg
     1381 gcattcaggc ggtcgaccag tgccagcagt tcttcctgcg atgttgattc gggcagctcg
     1441 taagacagtg atttgatgcc gcatttttgg caggcagttt tcttgttgcg gacataaacc
     1501 gcgctggcag ggtcgcctcc gaccaaaacc acggccaggc aaggcgtgtg cagattgtcc
     1561 tgttggcgtt gcgccaccgc ttcggcaacc gcctgcaggc gtttttgcga aacttcttta
     1621 ccattgatca gttgtgccga catatccgtc cttttgttaa acaggtagaa aatgctgaaa
     1681 attatcgcat tttccgtttg tttttacagc aggatgccga aaataaattt gccctgaaat
     1741 aaaatacggc tgtttggacg gattaaatat tttttcaaat caaaaaacta tatttttcat
     1801 cttggtgtct tgaaaataaa tattgacgca tctttccctg agttgtatag ttcgcttctt
     1861 caagtcgggg cgtagcgcag cccggttagc gcatctgctt tgggagcaga gggtcgtgag
     1921 ttcgaatccc accgccccga ccaatcaatc agcacgaatc ggcatgagcg cccgtagctc
     1981 aaccggatag agcaccgacc ttctaagtcg ggggttacag gttcgattcc tgtcgggcgt
     2041 gccaagaatt tgattgaaaa ccgtttttat ggtggctgta gctcagttgg tagagccccg
     2101 gattgtgatt ccggttgtcg tgggttcgag ccccatcagc caccccagat aaaggcccgt
     2161 acttttcggt acgggtcttt tcccgtttgc gcgtactgtc cgcgccgccg gctttgcctt
     2221 tcgcatatgc ttctgtatca tgtgggtttt tctgtctttt atggtttttg tcggaggctt
     2281 tgatggttga cgcttggtct gtccctgatt ttgaatcgaa aatctgtccg cccgaggcgt
     2341 tggcggcgcg tttggcgttg ttgccgcgcc cgctggtgtt taccaacggc tgtttcgaca
     2401 tcctccacag ggggcacgtt acttatctgg cgcaggcgcg ttcgatgggg gacgcgttgg
     2461 tgctggcgtt gaataccgat gcttcggtgc ggcgtctggg caagggcggt gaccgcccga
     2521 ttaatccttt ggagaaccgt gccgccgttg ccgccgcgtt ggaaagtgtg gatttggtaa
     2581 cgtggtttga cggggatacg ccggcggcgt tgattgaggc ggtcaaacct gagattttgg
     2641 tcaagggcgg cgattgggct gcggataaga ttgtcggtgc ggcagaaacg ttggcgcgcg
     2701 gcggtcaggt gttttcaatt ccgtttctgc accagacttc gacaacgaag actttggcaa
     2761 aaatccgtgc ggcagagggc ggaaaatgac ggttttgaag ctttcgcact ggcgggtgtt
     2821 ggcggagctt gccgacggtt tgccgcaaca cgtctcgcaa ctggcgcgta tggcggatat
     2881 gaagccgcag cagctcaacg gtttttggca gcagatgccg gcgcacatac gcgggctgtt
     2941 gcgccaacac gacggctatt ggcggctggt gcgcccattg gcggttttcg atgccgaagg
     3001 tttgcgcgag ctgggggaaa ggtcgggttt tcagacggca ttgaagcacg agtgcgcgtc
     3061 cagcaacgac gagatactgg aattggcgcg gattgcgccg gacaaggcgc acaaaaccat
     3121 atgtgtgacc cacctgcaaa gtaagggcag ggggcggcag gggcggaagt ggtcgcaccg
     3181 tttgggcgag tgtctgatgt tcagttttgg ctgggtgttt gaccggccgc agtatgagtt
     3241 gggttcgctg tcgcctgttg cggcagtggc gtgtcggcgc gccttgtcgc gtttaggttt
     3301 ggatgtgcag attaagtggc ccaatgattt ggttgtcgga cgcgacaaat tgggcggcat
     3361 cctgattgaa acggtcagga cgggcggcaa gacggttgcc gtggtcggta tcggcatcaa
     3421 tttcgtgctg cccaaggaag tggaaaacgc tgcttccgtg caatcgctgt ttcagacggc
     3481 atcgcggcgg ggcaatgccg atgccgccgt gctgctggaa acactgttgg cggaacttga
     3541 tgcggtgttg ttgcaatatg cgcgggacgg atttgcgcct cttgtggcgg aatatcaggc
     3601 tgccaaccgc gaccacggca aggcggtatt gctgttgcgc gacggcgaaa ccgtgttcga
     3661 aggcacggtt aaaggcgtgg acggacaagg cgttctgcac ttggaaacgg cagagggcaa
     3721 acagacggtc gtcagcggcg aaatcagcct gcggcccgac gacaggccgg tttccgtgcc
     3781 gaagcggcgg gattcggaac gttttctgct gttggacggc ggcaacagcc ggctcaagtg
     3841 ggcgtgggtg gaaaacggca cgttcgcaac cgtcggtagc gcgccgtacc gcgatttgtc
     3901 gcctttgggc gcggagtggg cggaaaaggc ggatggaaat gtccgcatcg tcggttgcgc
     3961 cgtgtgcgga gaattcaaaa aggcacaagt gcaggaacag ctcgcccgaa aaatcgagtg
     4021 gctgccgtct tccgcacagg ctttgggcat acgcaaccac taccgccacc ccgaagaaca
     4081 cggttccgac cgctggttca acgccttggg cagccgccgc ttcagccgca acgcctgcgt
     4141 cgtcgtcagt tgcggcacgg cggtaacggt tgacgcgctc accgatgacg gacattatct
     4201 cggcggaacc atcatgcccg gcttccacct gatgaaagaa tcgctcgccg tccgaaccgc
     4261 caacctcaac cggcccgccg gtaagcgtta tcctttcccg accacaacgg gcaatgccgt
     4321 cgccagcggt atgatggatg cggtttgcgg ctcggttatg atgatgcacg ggcgtttgaa
     4381 agaaaaaacc ggggcgggta agcctgtcga tgtcatcatt accggcggcg gcgcggcgaa
     4441 agttgccgaa gccctgccgc ctgcattttt ggcggaaaat accgtgcgcg tggcggacaa
     4501 cctcgtcatt cacgggctgc tgaacctgat tgccgccgaa ggcggggaat cggaacatac
     4561 ttaaaaaata cacccttttt caacacgggg cggcatccgt ccgaatccgt ccggattctg
     4621 ccgcccgaca gactgccgcc caggcgggcg gggtttcaaa cattgaggaa attctaatga
     4681 aatggctctt cacccttttg gcggtactca atattgccgt attcggcggc acggtgggct
     4741 ataaattggc cgttaaagcg acgggcggcg tgccggaaaa ccgggccgtc gaaaacacgc
     4801 cccccgcaac gcccgccgcc gacaatgcgg cggcatccgt ggaggatacg gcggcactgc
     4861 tcaaaccggg cgacatcctg accgaagagc aggccgagca gttgcgcttg aaaaaggaag
     4921 cggaacagaa aaaactgagg gagaaaaaac agcgcgagga aaaagcccgc cgcgaaaaac
     4981 tcgccgccga aaaggcgcag gcggaacgcg aaaacggcgc ggcggatgcc ttatgcgccg
     5041 cgcaggcaag cctcacgatg gacgaagatg actaccaccg catcaaaggg cttttgggca
     5101 aatggtcgca cgttgccagc aggagcgtcg aaaaacgcac cgccaaagcc aaacctgccg
     5161 acaaaaccta ccgcgtcgtc ctgcccgttt ccgccgatgc cgaaaatcag gcggcggagc
     5221 tgtctgccaa aggtttcaac cccataccgt ttgacggcgc attgagtttg ggtgtcggca
     5281 acagccggga aaacgcccaa gccctgcaaa accggcttgc cggcgccgga ttcggcgggg
     5341 cgcatattgt cgaacacttt gccgaagccg acaggcagga cgattctttg agcgtgtcgc
     5401 gtatgacggt tttgtttacc ggcgtgaatg ccgccgatgc ggacgaaatt cgtaaaatca
     5461 cgtccctata cggcaaactg aacctcaagt cttgcaaata ggcgcgaaag ccggacgata
     5521 aacgccaaac ccaccgcgaa ggtgggtttg gcgtttcggt tttgagtatc tgatatataa
     5581 aaagggaatg tatgtctcgt atatgccgac tccaagtgtg aaagtgaatt gtgagggaat
     5641 acagtgttta atggttcgga atggacgact tccgagtggt tttgattata cgctaataat
     5701 aataattatc aataaaaatt taaaaataaa tgcggatttt ttgtaatacg atgaaaaata
     5761 aacaaattaa cctgtccgcc gatgtttgtg attcgggtcg gcaaactttc attgtgtcgg
     5821 acaaatcggg gtcggcagtc atagctgagg gcttcgtgcc tgacggaaat aagcctgccg
     5881 tgtttgaaca tccgtccgat ttggcgcgga cagggttgtc attcgggggt gtttgagcgt
     5941 ggattcgggg atgcaaaagc ggtaaaaccg ttgcggatga aggcttacgg acggctttag
     6001 gtatttttaa gtgtattcaa acaacaacaa aacgcttctt ctattgccgt atttagggct
     6061 tgcgcggcag cctgcacacc gtggaggccg agtgtttcgt tggttgcggc atcgggacgg
     6121 gcggcaaggt tttccggccg gtttttccat tttttccgac agagccgtga tgcttgggaa
     6181 tggcggtttg cccgtttcag accgccgccg ttttgggctg cgatgccgtc tgaaccgttc
     6241 agacggcatt tttgccgcct ttttcaatat tccgccgaat gccaaaacgg ctgtccgact
     6301 gtcggcgtgc tggcttgcgc tttgtcgcgt gcttcgaccg gtccggcttc aaatgccagc
     6361 cgtccggatt cgacggcgag tgcgaaggcg cgcgccatat tgaccggatc gccgctgcgg
     6421 gaaacggcgg tgttcaaaag cacgccgtca aagccccatt ccatcacttg tgccgcctgt
     6481 gagggcaaac ccaagcccgc gtcgataatc agcggcgtgt cgggcaggcg ttcgcgcagg
     6541 acgttcaacg cgtaggcgtg aaccgcgccc aaacccgtgc cgatcggagc cgcccacggc
     6601 attaacgcct gacagcccac atcgagcagg cggcggcagg caatcaggtc ttcggtgcaa
     6661 taaggcagca ctttgaagcc gtctttaatc aggatttccg ccgcttcgac aagttggaac
     6721 acatccggct gcaaggtgtc gtcgtcgccg atgagttcga gtttaatcca atcggtttca
     6781 aacacttcgc gcgccatttg cgccgtcgtt accgcttcct gcacgctttg gcagcctgcc
     6841 gtgttcggca ggacgggaac gccggtttct tgaagcagcg accaaaaccc ctgaccgtgc
     6901 gcctcgccgc cgcttcccgt gcggcgcagc gagacggtaa tcatcgcggg ttgggcgata
     6961 cggacggatt gtttgaggat ttcaggggtc gggtaggcag ccgtgccgag cagcagccgt
     7021 gaagggaaag tttctccgta tagggtgagc ataatgggtt
//
LOCUS       DAXHRI010000104         7008 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXHRI010000104 DAXHRI010000000
VERSION     DAXHRI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.104"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(73..1494)
                     /locus_tag="ACFM75_001523"
     CDS             complement(73..1494)
                     /locus_tag="ACFM75_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC6256049.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1855..2214)
                     /locus_tag="ACFM75_001524"
     CDS             complement(1855..2214)
                     /locus_tag="ACFM75_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC6256050.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFYRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2211..2816)
                     /locus_tag="ACFM75_001525"
     CDS             complement(2211..2816)
                     /locus_tag="ACFM75_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC6256051.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2863..3345)
                     /locus_tag="ACFM75_001526"
     CDS             complement(2863..3345)
                     /locus_tag="ACFM75_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC6256052.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3549..3646
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3857..4957
                     /gene="gcvT"
                     /locus_tag="ACFM75_001527"
     CDS             3857..4957
                     /gene="gcvT"
                     /locus_tag="ACFM75_001527"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC6256053.1"
                     /translation="MITLKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            5120..5506
                     /gene="gcvH"
                     /locus_tag="ACFM75_001528"
     CDS             5120..5506
                     /gene="gcvH"
                     /locus_tag="ACFM75_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC6256054.1"
                     /translation="MSSNIPAELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDVLLTAEQYAGEVD"
     gene            5658..6905
                     /gene="hemA"
                     /locus_tag="ACFM75_001529"
     CDS             5658..6905
                     /gene="hemA"
                     /locus_tag="ACFM75_001529"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC6256055.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1786 a         1689 c         1963 g         1570 t
ORIGIN      
        1 tcggaagggg ggatattgat ttgattatgc cggaatttaa aatgccgtct gaatgttcag
       61 acggcatagc gtttacagca gtttgaaaac gaaaaagata agggtatgta cgatgaagac
      121 gggtgtcagg aaggcgaccg accacatcat atagccgaag aaagtcggca tcggtacgcc
      181 gcgctgttcg gcaatggcct tgaccatgaa gttcggtgcg ttgccgatgt aggtcagtgc
      241 gcccatgaat accgaaccca tagaaaccgc cagcagcgaa tgaaacaggg tacccgtcat
      301 caaggcttgg gcatcgccgc ccgccatatt gaaaaaaacg agataagtgg gcgcgttatc
      361 caagaatgcc gacaatatgc cgctcatcca aaaatacatc acattaatcg gatgacctgc
      421 cgtatcgtga accagcgata ccaccccgcc cagcgcgcct gcctcgcctg ctttcagaat
      481 gctcaggacg ggaaagatgg tgatgaagat gccgaggaag agtttgccca cttcggcgat
      541 gggttcaaag ttgaattcgt tgcctgcgcg gacttgtttg ggcgtgattg ccatagatac
      601 ggcggtcaat gcaatcagga tgacatcgcg gacgaggttt tgcagggcgt aacggctgcc
      661 gaggatttca aatcccgggt gttcgggttt ccaaaggccg gacattagaa ccgcgccgac
      721 cacgcccgaa agcaggagga agttccattt gccgaagatg gcgatttttt cgggtttttc
      781 ctgttgtgcc ggcgtatctt gtgcaatgct ttcctgtttg aagaaacggt tgtcgatgaa
      841 atagaaggcg gtcaacagga cagcggtgct gatcaggacg ggggcgaaca tatgtttgac
      901 cgtccacatg aaatctacgc ctttgaggaa gccgaggaag agtggggggt cgcccaaagg
      961 ggtcagaccg ccgccgatgt ttgcaaccag gaaaatgaag aagatgacga tgtgcacgcg
     1021 gcgggtacgg ttttggttgg ctttcagcag cggacgaatc atcagcattg ctgcgccggt
     1081 cgttcccatg atagaggcaa gtgccgtacc gacggcaagc agggcggtgt tgagcttggg
     1141 tgtgccgttc aagtcgcccc aaaccaaaat gccgcctgaa atggtgtaca gggcaagcag
     1201 cagcaggatg aaggggatgt attcttcaac gagtgcgtgt gcgacggtat ggataccggc
     1261 ggacgcgcca aaaaccaaac tgaacgggat gaggaagagc aatgtccaaa aggcggtgat
     1321 tttgccgtaa tggtgatgcc aggtatgcga aaaaaacaag ggacccaatg cgatagacag
     1381 caaaatcagg gcaaagggca ggctccacag caggtttagg tttgcgccgt ccaaatctgc
     1441 ggcgtaaacc gatgcgggga aaagcattag tgaaaacagg ggtaggtggc gcatcgtgtt
     1501 tcctcgattc aagcactgcc ttgcgcggcg cgtgggagtg atacaggcac cgtgccgccc
     1561 ggacataggc ggactgtgct gcctgtttgt ttttgaaaaa agttttccga ttgattgtaa
     1621 agtaaataat caggataaac cagtttccca aaccggaagg cggcgggaag gcggattgct
     1681 gtgcttggga atatatgttc tttttgataa ataattttat ttaaaacaaa taaatatatg
     1741 tctttaataa ataatctatc gaaaacgaaa aatgaattta ttttaacata tatttgcaat
     1801 gaaacaggtt tgcccccccc cgtttgtttg cccttatccc tttcagtacg gcattcaaga
     1861 ttcgggcctg cgccacatcc atatggcgac aagggaacaa aaaaccgatg aaaccgcccc
     1921 gacccaccag cgttggggaa actgccaaaa cattatcagg caggatgcgg tcatcatact
     1981 gatggcgaat attttggctt tgcgcggcac tgcgccgttt tgttcccagt tatgaaccat
     2041 cgggccgaaa tagcggtgcc ggtgcagcca gcggtaaaag cgcggggatg cctttgccca
     2101 gcaggcggcg gagagcagta cgaacggcgt ggtcggcaac agcggcaaaa aaatgccgat
     2161 gatacccaac agtagggaaa tgcagccgca ggcaattaaa agataacgta tcattttgaa
     2221 atatttttct tattgtgcgg ataagggcag gatgtgatac cgagttttgc ccagccttca
     2281 tgtcccattt tttccagcag ggcgatattg cgttcgaata ttgccgaagc gtcgggaaag
     2341 gcttgtgcgg ctttggcaat gctgtcttcg cggatgaggt gcagcgtcgg atagggagaa
     2401 cggttggtgt agttgccaat gtcgtctgaa tccgtgcctt caaattggaa atcgggatga
     2461 aacggggcga tttggacgat gccttctaag ccgttttcga caacggcggc atcggcaatg
     2521 tcgagcatat cgttgaatac gtcgaaatcg gggaataggg tcgggtgaac cagcagggtg
     2581 gtttccagtt cggtggcggg tgtattgccc agtcgctgca gttcttcgtc caagtcttcc
     2641 aaaaaaccgt caaggtgttt ggcttcgctg atcgcgatgc ggacaaggtt tttaacgtgg
     2701 ggggctttgg caaagggaca caggttcaga ccgatgacgg ctttttccaa ccattgtccg
     2761 gtgtgttcgg caacagcatc tttattttcg gaagtattga tattcattat tgtcatgtaa
     2821 atgtgtttgc agattgcacg tgcgggaaaa tcgggaaggg cactattcct tcagcaggtg
     2881 gttgagcggc agggaggtgg tgtgtttgat ttcttttaaa acaaagctcg attgcgcatc
     2941 ttgtacgccg tggtgggaca ggagcgtatc caaaacaaaa tgggaaaacg cgttcatatc
     3001 ggtaaaaaac gcctgaagca ggtagtcggt ttcccctgtc agggcgaagc agctcaagac
     3061 ttcaggccat tttcgaaccg atgcggcaaa gtcttcccgc gcgtcttttg ctttgcggat
     3121 ggaaacgcgg ataaatgcct gaagccccag gttgacggat tccggagaca gcagcgcggc
     3181 atattggcgg acgatgccgg catcttccaa ctgcttcaga cggcgcaggc acggagaagg
     3241 cgaaagtgcg acacgttcgg acagttcgac attggtcagc ctgccgtttt cctggagaac
     3301 ctgtaagatt ttaatatcgg ttttgtctaa agtgagttgg ggcatatttg cgttccgttt
     3361 taaggaattc ggattgtctg tccgtatgtt tgcggcaatc cgcacagatg gagaccatat
     3421 taacatataa aaagttatac cgtcatccgg gacaaatttt gttttcggaa aatcatgtga
     3481 aaacagaggc ggtcggtttg catctctttt aagacggctt gcccaaaccg ccgattcaag
     3541 acataatcgg gaaatgtgca ggagagtgtt acacccaact acaatgtaac caccgaaggc
     3601 gcagacaccc ttaaatcgct caggtatcag ggactgcaca ttgaaacaaa caatctggag
     3661 agcggcgttg gaataacgtc caccgaaggg gagaaggccg tctgaaccac cattcagaca
     3721 accgcgcaaa gcagtgagca gactggtttg ccatcatgcg gatacggccg aaaatctcag
     3781 gttcaaggac agatagggtc atccgcgcac aggtgcgcgg gtggcatctg aacaaaaaat
     3841 ccggagaaac ttgagaatga ttactctgaa aaccacccca tttcatcaag cccatcaaga
     3901 tgcaggcgcg aagctggtag attttgccgg ctgggagctg cccatccatt atggttcgca
     3961 aatcgccgaa cacgaagccg tgcgcaccga cgcaggtatg tttgacgtat cccatatgct
     4021 tgttaccgac gtagcaggcg caaatgccaa agcctttttc cgcaaattga ttgccaacga
     4081 tgtcgccaag ctcgcttttg tcggcaaagc cctttattcc gctttgctca acgacaacgg
     4141 cggcgtgatt gacgacttga tcgtttaccg caccaatgaa gccgaaaccc aataccgcat
     4201 cgtgtccaac ggcgcgactc gcgaaaaaga tacggcacaa ttccacaaag tcggacaaga
     4261 gttcggtgtt tccttcaatc cgcgctacga cctcggcatg ctcgccgtac aaggccctaa
     4321 agccattgaa aaactcctga ccgtcaaacc cgaatgggca gatgtcgtcc ataacctcaa
     4381 accgttccaa ggcgcggatt tgggcaacga ctggtttgtc gcccgcaccg gctacaccgg
     4441 cgaagacggc gtcgaagtca tcctgcccgg caccgaagcc gtcgcattct tcaaagccct
     4501 gcaagcagca ggcgtacaac cttgcggtct cggcgcgcgc gacaccctgc gcatggaagc
     4561 cggcatgaac ctctacggca acgatatgga cgacgatacc agcccgctcg aagcaggcat
     4621 gggttggacc gttgatttga aagacgaaag ccgcgatttt gtcggcaaag ctgccttgtt
     4681 ggcattgaaa gaaaaaggcg ttgccgtcaa acaggtcggt ttgctgctcg gcaaaggcgg
     4741 catcctgcgc gcgcatatgg aagtgttgac cgacaaaggc aaaggcgaaa ccaccagcgg
     4801 cgtattctct ccaagtctga aacaatccat cgccatcgcc cgcgtaccga aagattttga
     4861 cggcgatacc gccaaagtgc tgattcgcgg caaggaagcg gacgtgcgcg tactgaagct
     4921 gccgttcgtc cgcaacggtc aaaaacagtt tgattaatat cggttcagac ggcattttca
     4981 tttcatatgc cgtctgaaag caggttttaa ttgtcgtccg atacggacgt ttgtagaaag
     5041 cattgaacaa ggcatctgtg gatattgatt catgcagatg ccgtctgaaa ataaccccct
     5101 atcaatggag tatcaaacca tgagcagcaa catcccggca gaactgaaat acgttgccag
     5161 ccacgaatgg ctgcgccttg aagaagacgg tatcatcacc gtcggtatta cccaccacgc
     5221 gcaagagctg ttgggcgaca tcgtgtttgt cgagctgcct gaagtcggcg caaaccttgc
     5281 cgccgaagaa caagccggtg tggttgagtc tgtaaaagcc gcgtccgacg tgtacgcacc
     5341 gattgcaggt gaagtcgttg ccgtcaacga agatttgcca agcgctccgg aaactgccaa
     5401 cagcgatcct tacggtgcag gctggttctt caaactcaaa ccggcaaacg ttgccgatta
     5461 cgacgttctg ctgactgccg aacaatacgc gggcgaagtg gattaaaccg cccggctgcc
     5521 cgatggcaac cgccggacaa acggaaactg caccttcaga cggcattttt gcggtcggag
     5581 gtgcagtttt ttgtccgtgt tttaaggaag cagttaggct ataataacgg tctatattca
     5641 tctttaccga ttttttcatg caacttaccg ctgtcggact caatcatcaa accgcacctt
     5701 taagcatacg ggaaaagctg gcgtttgccg cggcctgcct gcccgaagcc gtccgcaatc
     5761 ttgcccgaag caatgcggca acggaggcgg taatcctttc tacctgcaac cgtaccgagc
     5821 tttactgtgt aggtgattcg gaagaaatca tccgttggct cgcagactat cacagccttc
     5881 ccatagaaga aatcagcccc tacctttata ctttggggat gcaggagact gtgcgccatg
     5941 ctttccgcgt cgcctgcggc ttggattcga tggtgttggg cgagccgcag attttaggac
     6001 agattaagga tgcggtcagg gttgctcaag agcaggaaag tatgggtaag aaactcaatg
     6061 ccctgttcca aaaaaccttt tctgttgcta aagaggtccg taccgatact gccgtcggcg
     6121 aaaactcggt ttccatggct tccgcttccg tcaagttggc agagcagatt ttccccgaca
     6181 tcggcgattt gaatgtcttg tttatcggtg cgggtgagat gattgagctg gttgccactt
     6241 attttgccgc caaaagtccc cggctgatga cggttgccaa ccggacgctg gcgcgtgcac
     6301 aggagttgtg cgacaagctc ggtgtcaacg ccgaaccgtg cctgctgtcc gatctgcctg
     6361 ccattttgca tgagtacgac gtggtggttt cttcaacggc aagccagttg cccattgtcg
     6421 gcaaaggtat ggtggagcgc gcattgaaac aaaggcagag tatgccgttg tttatgcttg
     6481 acttggccgt gccgcgagac attgaggcgg aagtcggcga tttgaacgat gcctatcttt
     6541 atacggtgga cgatatggtc aacatcgtcc aaagcggcaa ggaggcaagg cagaaggccg
     6601 ccgccgccgc cgaaacgctg gtatccgaga aggttgccga atttgtcagg cagcagcagg
     6661 gcaggcagag cgtccccttg attaaagcct tgcgggacga gggcgagaaa gcgcgcaagc
     6721 aggtgttgga aaatgcgatg aaacagcttg ccaaaggcgc aacggcagaa gaggttttgg
     6781 aacggctgtc cgtccaactg accaacaagc tgctgcattc gccgacccaa actttgaata
     6841 aagcggggga agaagataaa gatttggttc atgccgtcgc gcagatttat catttggaca
     6901 aataacggtg cgccggaaaa ggatgcgtct gaaggggttt cagacggcat atttgtgtgc
     6961 cgcctgacaa catcgtgaaa tcccacatta tatcgatgta atcacaaa
//
LOCUS       DAXHRI010000105         6992 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXHRI010000105 DAXHRI010000000
VERSION     DAXHRI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.105"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(1..219)
                     /locus_tag="ACFM75_001530"
                     /pseudo
     CDS             complement(1..219)
                     /locus_tag="ACFM75_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(248..577)
                     /locus_tag="ACFM75_001531"
     CDS             complement(248..577)
                     /locus_tag="ACFM75_001531"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6256056.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(655..873)
                     /locus_tag="ACFM75_001532"
     CDS             complement(655..873)
                     /locus_tag="ACFM75_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6256057.1"
                     /translation="MLKTLTFAALHFSTAFSITYVLTGSIGISGTVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCG"
     gene            995..1948
                     /locus_tag="ACFM75_001533"
     CDS             995..1948
                     /locus_tag="ACFM75_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6256058.1"
                     /translation="MNQKKVVVLDADTLPGRTFHFDFPHELAVYGTTDAAETAGRVRD
                     AHIIITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIQDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMEVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2025..4082)
                     /gene="metG"
                     /locus_tag="ACFM75_001534"
     CDS             complement(2025..4082)
                     /gene="metG"
                     /locus_tag="ACFM75_001534"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC6256059.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAHDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESQYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     AFYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(4198..6036)
                     /gene="glmS"
                     /locus_tag="ACFM75_001535"
     CDS             complement(4198..6036)
                     /gene="glmS"
                     /locus_tag="ACFM75_001535"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC6256060.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRTIADTAEVFLD
                     GGFIPENFGKDAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            6141..6266
                     /locus_tag="ACFM75_001536"
     CDS             6141..6266
                     /locus_tag="ACFM75_001536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256061.1"
                     /translation="MPSEGLGLLKKGRDFPVYPRWAFRRHCLPPVYSFYKSILTS"
     gene            6317..6838
                     /locus_tag="ACFM75_001537"
     CDS             6317..6838
                     /locus_tag="ACFM75_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256062.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGELSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGRSPALLL"
BASE COUNT         1462 a         1669 c         2103 g         1758 t
ORIGIN      
        1 gcaaccggta gatgaatcat cgcggcaggt tgacggcagg tgcttggtgt tgattttgtc
       61 gtcggtcttg ccgttggcgg cggcgacgtc ggtggcggtg gcggtgtcgt tgcgcgtaac
      121 cggctgtccg cagaaccatt ttaccgaacc ggcttgacgc ttggcccaca gggagagttt
      181 tttgtctttg atttcgttgt ttacgttgct tgaagccatt atgtcagacg gtattgcccg
      241 ggcagcttta ttcgtacact ttcagcagct cgacttcaaa tatcaaagtg gcgtgcgggg
      301 gaatcacgcc gcccgcgccg tgtgcgccgt agcccatttc cgaagggatg gtcagcttgc
      361 gtttgccgcc ttccttcatt ccgccgaagc cttcgtccca gcctttgatg acttgtccga
      421 caccgagcgt gatggtcagc ggctggcggc ggtcgaggct ggagtcgaat ttggttccgt
      481 tttccagcca acctgtgtaa tgcacggtaa tctctttgcc tttaactgct tcttttccga
      541 agccttcctg caaatcttca ataatcaggc tgcccatatt tgtcctttcg ttgcttgttg
      601 gtcaaaacgg caagggtaac ataccgtccg gcgaagtcaa atgccgctca aacgtcagcc
      661 gcaccggtgc agttgaaacg gctgtgtttg ttggactgtt ttattttttt cgtaaaggtt
      721 ccatgctttt tcatggaaat agaaaacgac ggtgttgatt aggggctcga ccagcgcgac
      781 cgtcccggat atgcctatac tgcccgtcag tacataggtt atactgaagg cggtgctgaa
      841 atgcagtgcg gcaaaagtca gggttttaag catcatcctc tcccgaattg gacattgacg
      901 gagggatgat aaagattatc ataaggctgt gcggtttaaa tttgctattt gctgttagtg
      961 taaataaatc attttctaaa tagggataaa aattatgaat cagaagaaag tagtcgtatt
     1021 ggatgcggat actttacccg gtcggacttt tcacttcgat ttcccacacg agcttgcggt
     1081 ttacggtacg acagatgctg cggaaacggc agggcgggtg cgcgacgcgc atattatcat
     1141 caccaataag gtgatgattt ccgccgacat tattgcggct aatcctcagc ttgagctgat
     1201 tgccgtcagt gcgaccggtg tgaacaatgt cgatattggg gcggcggagg cggcgggcgt
     1261 tgcggtatgc aatgtccgcg cctacggaaa cgaatcggtt gcggaacatg cctttatgct
     1321 gatgattgcc ctgatgcgga atttgcctgc ctatcagcgt gatgttgcgg caggattatg
     1381 ggaaaaatcg ccgtttttct gccattacgg cgcgccgatt caggatttga acggcaaaac
     1441 gctggcggtt ttcggacgcg gcaatatcgg acggacgctt gccggatacg cgcaggcatt
     1501 cggtatggag gtggtgtttg ccgaacacaa acacgcgtcc gctgtgcgtg aaggctatgt
     1561 ttcctttgaa gatgcggtac gggctgctga tgtgttgtcg ctgcactgtc cgctaaacgc
     1621 ccaaactgaa aatatgatag gcgaaaacga attgcggcag atgaagcctg gcgcggtttt
     1681 aatcaattgt gggcgcggcg ggctggtgga tgaaaacgcg ctgcttgccg cactcaaata
     1741 cgggcagatc ggtggggcag gtgtcgatgt tttgacgaat gagccgccca aaaacggcaa
     1801 tcccttgctg aatgcacgcc tgcccaatct gattgttacg ccgcataccg cgtgggcaag
     1861 tcgtgaggct ttggacaggc tgtttgatat attgttggcg aacattcacg cctttgtgaa
     1921 aggagaggcg caaaaccgcg tggtttgaac ctgtcgggat tgcggaaaaa aatgccgtct
     1981 gaacgcctca agggttcaga cggcattgtt tttgatattt ctacttaacc gactttgtcg
     2041 cccggttgcg cgcctgtatc cacatccaag agcttcagtt tcccgtctgc cgtggcggcg
     2101 gagagaatca tgccttcaga tacgccgaat tttgccattt tgcgcggggc gaagttggcg
     2161 acggcaatga ccattcttcc gtttaactcg gcagggttgg ggtaaaacgc ggcgatgccg
     2221 gagaagatga tacgtttttc aaaaccgaaa tcgaggtcga atttcaggag tttggtgctg
     2281 ccttcgacag cttcgcagtt caatactttg gcaacgcgca tgtcgatttt cataaagtcg
     2341 tcgaagctcg cctgttcggc aactttttcg tattgacttt cttcggcggc aggtgcggct
     2401 gcggcggcga tgctttgttt gttggcttcg attaaatcgt ccacttgttt ttgctccact
     2461 cgttgcatga gatgttcgta tttgttgatg gcgtgttcgc ccaaggtttc gcgtgtattt
     2521 gcccaagtga tggcttccaa attcaggaat ttggcggcgt ttgcggcggt ttgcggcaag
     2581 acgggggcga ggtaggcggt caacatggtg aaggcgttga tgagctcgct gcacacttcg
     2641 tgcaggcgtt cgtcttgacc ttcctgtttc gccagttccc aaggtttgtt ggcatcaacg
     2701 tattcgttca cggcatctgc caatgccatg atatcgcgca gggcgcgggc gtattcgcgg
     2761 ttttcgtatt gctcggcgat ggtgtcgctt tcggcggcaa gttttgccag caatgcgctg
     2821 tcggcaacgt ctttcagacg gccttcaaag cgtttggcga taaaacctga ggcgcgggcg
     2881 gcgatgttga cgtatttgcc gacaaggtcg ctgtttacgc ggctgataaa gtcttgcagg
     2941 ttcaaatcga tgtcttcgat tttgctgttg agtttggcgg cgatgtagta gcgcatccac
     3001 tcggggttta aaccttgttc caaataggat ttggcggtga taaacgtgcc gcgcgatttg
     3061 gacatttttt gtccgtccac ggtcaaaaag ccgtgtgcgt acacgccggt cggggcgcgg
     3121 tggccggaga aatgcagcat ggcaggccag aacagggcgt ggaaatagag gatgtctttg
     3181 ccgatgaagt ggtacatctc ggtttggctg ccggctttga agtattcgtc gaaatcgacg
     3241 ccgatgcggt cgcacaggtt tttaaacgac gccatgtagc cgacgggcgc gtccagccag
     3301 acgtagaagt atttgcccgg cgcgtcgggg atttcaaaac cgaaatacgg cgcgtcgcgg
     3361 gaaatgtccc agtcggacag ggtggtttct tcgccttcgc ccagccattc tttcattttg
     3421 ttgagggctt ctggttgcag atggggtttg ccgtcgtgcg ggttgttgcc ggaagtccat
     3481 gctttgagga agtcggcgca ttcgcccagt ttgaagaaga agtgttcgga ttcgcgcaat
     3541 tcgggtttcg cgccggaaac ggcggaatac gggttgatca gttcggtcgg ggaataggtc
     3601 gtgccgcaga cttcgcagtt gtcgccgtat tggtcgtggg cgtggcattt ggggcattca
     3661 cctttgacga aacggtcggg caggaacatt tgtttttcgg ggtcgaaaag ctgctcgatg
     3721 acgcggctct caatcttgcc gttggctttc agcgcgcggt aaatgtcttg ggaaaactgt
     3781 ttgttttcag gggaatgggt gctgtaataa ttgtcgtagc cgatgttgaa accggtaaag
     3841 tcggcaaggt gctcttcgcg cactttggca atcatgtctt caggcgcgat gccttgtttt
     3901 tgcgcggcaa gcatcacggg cgtgccgtgg gtgtcgtcgg cgcagcagta gtggcattcg
     3961 tggccgcgca gtttttgaaa gcgcacccaa acgtcggttt ggatgtgttc gaccatgtgg
     4021 ccgaggtgga tgctgccgtt ggcatagggc agggcggagg taactaagat tttgcgtgtc
     4081 atattgtgct ttgcaaacaa tgggtaaagg cggattatac cgcaaatcaa acggggaaat
     4141 gccgtctgaa gcctgaaaaa tcaggcttca gacggcattt ttgccaaccg gcgggattta
     4201 ttcgacggtt acggattttg ccagattgcg cggtttgtcc acatccgtgc ctcgggcgag
     4261 ggcggtgtgg taggcgagaa gctgcacggg gatagtatgc acgacggggg acagttcgcc
     4321 gacatggcgc ggcgcacgga taacgtgcac gccttcggtg gcattaaaat tgctgtcgag
     4381 gtcggcaaag acgaaaagtt cgccgccgcg cgcgccgact tcctgcatat tggctttgac
     4441 tttgtccaac aggctgtcgt tgggcgcgat gacgacgacg ggcatatttt cgtccaccag
     4501 ggcaagcggc ccgtgtttca gctcgccggc aggataggct tcggcgtgga tgtaggtgat
     4561 ttccttcagc ttcaacgcgc cttcgagggc aatcgggtaa tggatgccgc gccctaaaaa
     4621 cagcgcgctg gttttcttgg caaactgttg cgcccaggcg gcaatttgag gttcgaggtt
     4681 taaagcgtgc tgcacgctgc cgggaagctg gcggagttct tcggtgtaac gtgcttcatc
     4741 ttcgccggat accaaaccgc gcactttcgc cagcgttacc gccaaaccga acagcgcgac
     4801 cagctgcgtg gtaaacgcct tggtcgaggc gacgccgatt tccgcaccgg cacgggtata
     4861 aagcacgagg ctgctttcgc gcggcagggc ggattccatc acgttgcaga tggagaggct
     4921 gtggcggtgt cccaaagatt tggcgtattt caacgcctcc atcgtgtcca gcgtttcgcc
     4981 ggattgggaa atggtaatga ccagttggtc cggatcggca atcacgctgc ggtatcggta
     5041 ttcgctggcg atttcgacgt cggtcggaat tttggcgatg gattcgagcc agtattttgc
     5101 ggtcagcgcg gcgtaatagg acgtgccgca ggcaaggatt ttgacgctgc ggatgctttc
     5161 aaacacgctt ttggcgtctt tgccgaagtt ttcgggaatg aagccgccgt cgaggaaaac
     5221 ttccgccgta tccgcaatcg tgcgcggctg ctcgtggatt tccttttgca taaagtggct
     5281 gtacggtccc agttccaaag aggcgagcga gagttcggat accttgactt tgcgttcggc
     5341 aggcaggccg tttttatcgg tcagcctttt gatgccgtct gaagccagca gcgcgatgtc
     5401 gccgtcttcg aggtacgcca cgcggcgcgt aaaggcgatg acggcggata cgtccgaagc
     5461 gataaaggtt tcatcgtcgc ccaaagcgac caaaagcggg cagcccatac gcgccacaac
     5521 taattcatca ggcttgtctt gggcaataac cgcgatggcg tatgcgccgt ggaaacgttt
     5581 gaccgcttct tgtaccgctt caaacagcct gccgccgttt tgcgcgtatt cgtgattgat
     5641 gctgtgggcg atgacttcgg tatccgtttg cgattcaaaa cggtatccca aaccttccaa
     5701 acgtttgcgt tcgctttcaa agttttcgat gatgccgttg tgtacgaccg caatcatccc
     5761 gccgctgatg tgcgggtggg cgttcggctc ggtaacgccg ccgtgtgtcg cccagcgcgt
     5821 atgcccgatg ccgatgccgc cgctgatgcc tttttcgcgt gccgcgtcct ccataagctg
     5881 cacgcgtccg acgcggcgca cgcgtttgat tttgccgtcg gtgttgacgg caatgcctga
     5941 tgagtcataa ccccggtatt cgaggcgttt gagaccgtcg gtcagaaaat cgacgacgtt
     6001 gtgatgggcg cggatggcgc cgacgatacc gcacataact gttccttagt atccggttga
     6061 aaaaaacagg cgcggacggc tcccgtgccg caccttcctc ttcggattat aaaccgcctc
     6121 cagcgccgaa aaacagcaaa atgccgtctg aaggcttggg cttgctcaaa aaagggaggg
     6181 atttccctgt ttatccgaga tgggcgttca gacggcattg cctgccgccg gtttatagtt
     6241 tttacaaatc aatattgaca agctgaaaaa aaaaaacaat atactcgctc ggtcttaatg
     6301 ttaaaggagt atggaaatga aacaaatgct tttggccgtc ggcgtggcgg cggtgttggc
     6361 aggctgcggc aaggatgccg gcggttacga gggttattgg cgcgaaaaat cggacaaaaa
     6421 agagggtatg attgccgtca aaaaagaaaa aggcaattac ttccttaata aaatcaacgt
     6481 gtttacaggt aaggaagaat ctatgctttt gtctgaaaaa gacggcgagc tttcgataaa
     6541 cacggggata ggggaaatcc cgatcaaact ttccgacgac gggaaagagc tgtatgtcga
     6601 acgcaggcag tatgtcaaaa ccgatgcggc gatgaaggac aaaatcattg cccatcagaa
     6661 aaagtgcgga caaacggcac aggcatatct cgacgcgcga aatgcgttgc cgtcaaacca
     6721 gacgtatcag cagcatcagg cggcgatcga gcaattgaaa cggcggtttg aagccgagtt
     6781 tgacgaattg gaaaaagaaa tcaaatgcaa cggcagaagc ccggcattgt tgctttagta
     6841 ggggacaacc gggaggatgc cgccgtccga atcggatgtg cggtttctgt accggtacgg
     6901 gcgggcagga atgtccgcct tttttgttcg gatgcgtttg aatacccgtt tgattccgac
     6961 cgtttgcaag gggtatttcc gttcgggcgg aa
//
LOCUS       DAXHRI010000106         6969 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXHRI010000106 DAXHRI010000000
VERSION     DAXHRI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.106"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            56..1294
                     /gene="mtrC"
                     /locus_tag="ACFM75_001538"
     CDS             56..1294
                     /gene="mtrC"
                     /locus_tag="ACFM75_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC6256063.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIASITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
     gene            1306..4509
                     /gene="mtrD"
                     /locus_tag="ACFM75_001539"
     CDS             1306..4509
                     /gene="mtrD"
                     /locus_tag="ACFM75_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC6256064.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLVGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            4564..5967
                     /gene="mtrE"
                     /locus_tag="ACFM75_001540"
     CDS             4564..5967
                     /gene="mtrE"
                     /locus_tag="ACFM75_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC6256065.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWL
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALTAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(6159..6356)
                     /locus_tag="ACFM75_001541"
                     /pseudo
     CDS             complement(6159..6356)
                     /locus_tag="ACFM75_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(6356..6562)
                     /locus_tag="ACFM75_001542"
                     /pseudo
     CDS             complement(6356..6562)
                     /locus_tag="ACFM75_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
BASE COUNT         1572 a         1833 c         1949 g         1615 t
ORIGIN      
        1 aatgcctgtt gattggcaaa accctttaca tcaaaccaga cagataggat aaataatggc
       61 tttttatgct tttaaggcga tgcgtgcggc cgcgttggct gccgccgttg cattggtact
      121 gtcgtcttgc ggtaaaggcg gagacgcggc gcagggcggg cagcctgctg gtcgggaagc
      181 ccctgcgccc gtcgtcggtg tcgtaaccgt ccatccgcaa accgtcgcgt tgaccgtcga
      241 gttgccgggg cgtttggaat cgctgcgtac cgccgatgtc cgcgcccaag tcggcggcat
      301 catccaaaaa cgcctgttcc aagaaggcag ttatgtccgt gccggacagc cgctgtatca
      361 gatcgacagt tccacttatg aagcaggtct ggaaagcgcg cgcgcgcaac tggcaacggc
      421 tcaggcaacg cttgccaaag cggatgcgga tttggcgcga tacaagcctt tggttgccgc
      481 cgaagccgtc agccggcagg aatacgatgc tgcggtaacg gcgaaacgtt ctgccgaggc
      541 aggtgtcaaa gcagcacagg cggcaatcaa atctgccggc attaatctga accgttcgcg
      601 cattaccgcg ccgatttccg gctttatcgg tcagtccaaa gtttccgaag gtacgctgtt
      661 gaatgcgggc gatacgaccg tgctggcaac catccgccaa accaatccga tgtatgtgaa
      721 cgttacccag tctgcatccg aagtgatgaa attgcgccgt cagatagccg aaggcaaact
      781 gctggcggcg gatggtgtga ttgcggtcgg catcaaattt gacgacggca cagtttaccc
      841 tgaaaaaggc cgcctgctgt ttgccgatcc gaccgtcaac gaatcgaccg gtcagattac
      901 cctgcgcgcc gccgtaccga acgatcagaa tatcctgatg cccggcctgt atgtgcgcgt
      961 gctgatggac caagtggcgg tggataacgc atttgttgtg ccgcagcagg cggtaacgcg
     1021 cggtgcgaaa gataccgtga tgattgtgaa tgcccaaggc ggtatggaac cccgcgaggt
     1081 aacggttgcg caacagcagg gtacgaattg gattgttacg tcgggtctga aggacgggga
     1141 caaggtggtt gtggaaggca tcagtatcgc cagtataacg ggtgcgaaaa aggtaacgcc
     1201 caaagaatgg gcgccgtctg aaaaccaagc cgccgcgcct caatccggcg ttcagacggc
     1261 atctgaagcc aaaactgctt ctgaagcgga ataaggaagg catcgatggc taaatttttt
     1321 atcgaccgcc ctatttttgc gtgggttatt tcgattttca ttattgcggc gggtattttc
     1381 ggcatcaaaa gcctgcctgt ttcgcaatat ccgtctgtcg ccgccccgac cattactttg
     1441 cacgccactt atccgggtgc gtccgcgcag gtaatggaag acagcgtgct ttccgtgatc
     1501 gagcggaata tgaacggcgt ggaaggtttg gattatctgt ccacttccgc cgattcgagc
     1561 ggcagcggca gcgtgagcct gacctttacg cccgataccg acgagaatct ggcgcaggtg
     1621 gaagtgcaga acaagctttc cgaagtattg agcacgctgc cggcaaccgt ccagcaatac
     1681 ggcgtaaccg tatccaaggc gcgttccaat ttcctgatga ttgtgatgct ttcgtcggat
     1741 gtgcagtcaa ccgaagagat gaacgactac gcgcagcgta atatcgttcc cgaactgcag
     1801 cgtatcgaag gcgtggggca ggtacgcctg ttcggcgcgc aacgcgcgat gcggatttgg
     1861 gttgatccta agaaactgca aaactacaat ttgtcgtttg ccgatatcgg cagtgcgctg
     1921 tccgcccaga acgtccagat ttcagcgggt tctatcggtt cgcttcccgc cgttcgcgga
     1981 cagacggtta cggctaccgt aacggcgcaa gggcagttgg gtacggcaga agaattcggc
     2041 aacgtcatcc tccgcgccaa taccgacggt tctaatgttt acctgaagga tgtggcaagg
     2101 gtcggactgg gtatggaaga ctattcttcc tcaacccgtc tgaacggtgt aaataccacc
     2161 ggtatggcgg tgatgctgtc caacagcggc aatgcgatgg cgacggcaaa ggcggttaaa
     2221 gaacgcatgg cgacgttgga aaaatacttt cctcagggta tgagctggaa aaccccttac
     2281 gatacttcca aattcgtcga aatttcgatt gaaaaagtga ttcatacttt aatcgaagcg
     2341 atggtgctgg tgtttgtcgt aatgtatctc ttcctgcaaa acatccgtta tacgctgatt
     2401 ccgaccatcg tcgtaccgat ttcgctgttg ggcggtttcg ccttcatctc ttatatgggc
     2461 atgtcgatta acgtactgac catgtttgcg atggttttgg tcatcggcat cgtggtcgat
     2521 gacgcgattg tggtggttga aaacgtcgag cgcattatgg cgggtgaagg cttgccgccc
     2581 aaagaagcga ccaaaaaagc gatgggtcag atttcgggcg cggtcatcgg tattaccgcc
     2641 gttctgattt ccgtgttcgt accgttggcg atgttcagcg gggcgacggg caatatttac
     2701 aaacagtttg ccctgacgat ggcgtcatca atcgcattct ccgccttcct tgcgctgacc
     2761 cttacccctg ctttgtgtgc cacaatgttg aagacaatcc cgaaagggca tcacgaagag
     2821 aaaaaaggtt tcttcggctg gtttaacaag aaattcaaca gttggacgca cggttacgaa
     2881 ggccgggttg ccaaagtgct gcgtaagact ttccgcatga tggttgtcta tatcggcttg
     2941 gcggttgtgg gcgtgttcct gtttatgcgc ctgccgactt cgttcctgcc gaccgaagac
     3001 caaggcttcg tcatggtcag cgtacaactg cctgcggggg cgacccaaga gcgcaccaat
     3061 gcgactttgg cgcaagttac ccaactggcg aaaagcattc ctgaaataga aaacatcatt
     3121 accgtttccg gcttcagctt ttcgggcagc ggtcagaata tggcgatggg ttttgccata
     3181 ttgaaagatt ggaacgagcg taccgcgccc ggcagcgatg ccgttgcgat tgccggcaag
     3241 ctgacgggta tgatgatggg gacgcttaaa gacggttttg gcatcgccgt cgtcccgcct
     3301 ccgattctgg agttgggcaa cggttcgggc ctgagcatca acctgcaaga ccgcaacaat
     3361 accggccata ccgcattgct ggcgaagcgc aacgagttga ttcagaaaat gcgtgccagc
     3421 ggtttgtttg accccagcac cgtccgtgcc ggcggtttgg aagactcgcc gcagttgaaa
     3481 atcgacatca accgtgccgc cgcggcggcg caaggcattt cgtttgccga catccgcacc
     3541 gcattggcaa gcgcgctgag ttcgtcttat gtcagcgact tcccgaacca aggccgtctg
     3601 caacgcgtga tggtgcaggc cgacgaggat gcccgtatgc agcctgccga tattttgaac
     3661 ctgaccgtgc cgaacaaatc cggcgtcgcc gtaccgcttt ccaccattgc tactgtttct
     3721 tgggaaaacg gtacggaaca gagcgtacgc ttcaacggtt atccttcgat gaagctgtcc
     3781 gcttcgcctg caaccggcgt ttccaccggg caggctatgg cggcggttca aaaaatggtt
     3841 gacgaattgg gcggcggtta cagcttcgaa tggggcggac agtcgcgcga agaggcaaaa
     3901 ggcggctcgc aaaccctgat tttgtacggt ttggcggttg cagccgtatt tttggtactt
     3961 gccgcgcttt atgaaagctg gtcgattccg ctggcggtca tccttgtgat tccgttgggt
     4021 ttgatcggtg cggctgcggg cgtaaccggg cgcaacctgt ttgaaggact gttgggcagc
     4081 gttccctcgt tcgccaacga catctacttt caagtcggtt tcgttaccgt gatgggtttg
     4141 agtgcgaaaa acgcgatttt gattatcgaa tttgccaaag accttcaagc gcaagggaaa
     4201 agcgcggtcg aagctgcttt ggaagccgcc cgcctgcgtt tccgtccgat tatcatgacc
     4261 tcgttcgcct ttattttggg cgtggttccc ctgtatattg ccggcggtgc aagttctgcc
     4321 agccagcgcg ccatcggcac gaccgtattc tgggggatgt tggtcggcac gctcttgtcc
     4381 gtgttccttg ttccgctttt ctatgtggtg gtgcgcaaat tcttcaaaga aaccgcgcac
     4441 gaacacgaaa tggcagtaaa acacgccgcc gaagcgggca ttaccggttc ggacgacagc
     4501 caacattaag caaccatgcc gtctgaacgc ccacgggttt tcagacggca tcaggacttt
     4561 tttatggata ctacattgaa aaccaccttg acttctgttg cagcagcctt cgcattatcc
     4621 gcctgcacca tgattcccca atacgagcag cccaaagtcg aagttgccga aacgttcaaa
     4681 aacgataccg ccgacagcgg catccgcgcc gtcgatttag gttggcatga ctattttgcc
     4741 gacccgcgcc tgcaaaagct gatcgacatc gcactcgagc gcaataccag tttgcgtacc
     4801 gccgtattga acagcgaaat ctaccgcaaa caatacatga ttgagcgcaa caacctcctg
     4861 cccacgcttg ccgccaatgc gaacgactcg cgccaaggca gcttgagcgg cggcaatgta
     4921 agcagcagct acaaagtcgg actgggtgcg gcatcttacg aactcgatct gttcgggcgt
     4981 gtacgcagca gcagcgaggc ggcactgcaa ggctatttcg ccagcaccgc caaccgcgat
     5041 gcggcacatt tgagcctgat tgccaccgtt gccaaagcct atttcaacga acgttacgcc
     5101 gaagaagcga tgtctttggc gcaacgtgtt ttgaaaacgc gcgaggaaac ctacaagctg
     5161 tccgaattac gttacaaggc aggcgtgatt tccgccgtcg ccctacgtca gcaggaagcc
     5221 ctgatcgaat ctgccaaagc cgattatgcc catgccgcgc gcagccgcga acaggcgcgc
     5281 aatgccttgg caaccttgat taaccaaccg atacccgacg acctgcccgc cggtttgccg
     5341 ttggacaagc agttttttgt tgagaagctg ccggccggtt tgagttccga agtattgctc
     5401 gaccgtcccg atatccgtgc tgccgaacac gcgctcaaac aggcaaacgc caatatcggt
     5461 gcggcacgcg ccgccttttt cccatccatc cgcctgaccg gaagcgtcga tacgcattct
     5521 gccgaattgg gcgggctgtt caaaagcggc accggcgttt ggttgttcgc accttccatt
     5581 accctgccga tttttacctg gggtacgaac aaggcgaacc tcgatgtggc aaaactgcgc
     5641 caacaggcac aaatcgttgc ctatgaatcc gccgtccaat ccgcatttca agacgtggca
     5701 aacgcattga ccgcgcgcga gcagttggat aaagcctatg acgctttaag caaacaaagc
     5761 cgcgcctcta aagaagcgtt gcgtttggtc ggtctgcgtt acaaacacgg cgtatccggc
     5821 gcgctcgact tgctcgatgc ggaacgcagc agctattcgg cggaaggtgc ggctttgtcg
     5881 gcacaactga cccgcgccga aaaccttgcc gatttgtaca aggcactcgg cggcggattg
     5941 aaacgggata cccaaaccga caaataaccg gtcgggcaat aaaatgccgt ctgaaacgat
     6001 aaggtttcag acggcatttc tttctttata gtggattaaa aaaaaccagt acagcgttgg
     6061 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt tgtactattt
     6121 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat atgtttgacg
     6181 gttgcggtca ggctgcgcgg cgtgccgtaa acgtgaatgt cgggcatagg gcggtagcgt
     6241 ttgttgaaca cgttttcaaa gtccaaaccg atgcgggtgg atttgccgat tttgtaatgc
     6301 gcggtcaaat ccagcgtggc gtaggggcgt tgggtcagtg cggctgcggc ggcagggaag
     6361 aaaccgccat acccgccggc atcccgaagc cgaatacggc aacgacaagc gcaatcagca
     6421 gcgtataaag ccccgcctga tagcctttca gcttcaggac ggtcagcgcg gcaaagaaaa
     6481 agacgatggg taagagtgcg gcggcggcag tcagatacag gctgccgccg attgccgtgt
     6541 agttttgaac ccaagtttcc ataattgaac atctccgaaa atatttttct aatcgtcggc
     6601 aatagtggtc aaaccaatta aagcaacgtt gcattacttt gcgaaacttt aatattcagg
     6661 tcaatagaat ggattaaatt taaaccagta cggcgttgcc tcgccttacc gtactatctg
     6721 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata tatttttggg
     6781 cggttcggca gatttgaatc ggagctttcg tttaaatcct gtcaaaacaa atatttgtat
     6841 aaacaaaaat tgtagtttgg tgtagttttt tcttgtgttt cgggggcggt gcgaggtaaa
     6901 tgccgttgac ggcgggaacg gcgtgtcatt agaatccgcc ctgattggtc agtccaattt
     6961 gatgtttga
//
LOCUS       DAXHRI010000107         6863 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXHRI010000107 DAXHRI010000000
VERSION     DAXHRI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.107"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(1..118)
                     /locus_tag="ACFM75_001543"
                     /pseudo
     CDS             complement(1..118)
                     /locus_tag="ACFM75_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(275..1576)
                     /locus_tag="ACFM75_001544"
     CDS             complement(275..1576)
                     /locus_tag="ACFM75_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC6256066.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPI
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELNKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(1566..2477)
                     /locus_tag="ACFM75_001545"
     CDS             complement(1566..2477)
                     /locus_tag="ACFM75_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6256067.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            2585..2983
                     /locus_tag="ACFM75_001546"
     CDS             2585..2983
                     /locus_tag="ACFM75_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6256068.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            3032..3637
                     /locus_tag="ACFM75_001547"
     CDS             3032..3637
                     /locus_tag="ACFM75_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6256069.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            3823..5934
                     /locus_tag="ACFM75_001548"
                     /pseudo
     CDS             3823..5934
                     /locus_tag="ACFM75_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            6049..6744
                     /locus_tag="ACFM75_001549"
     CDS             6049..6744
                     /locus_tag="ACFM75_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFC6256070.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         1606 a         1783 c         1907 g         1567 t
ORIGIN      
        1 tacgggtatg ttgtttgtaa ccgagtctgt agaggccgtt tttcttgatc caacgggcat
       61 ctttgtcctt actcggtgtg gtttggccgt tgatttgtcc ttcttcatcg acttctattt
      121 ttgccgacga tgggaacggt ggcggcttgg gtggcggtga tttgggcata cctgatgatt
      181 gaaagtgaaa aaaacggaag atattgagtc attcggacgc aatgccgtct gaaacggaag
      241 ttcagacggc atttgtttta ggttgccgta ccgcttaggg aataccggcg acaggatggg
      301 cgggatagcc gtgggtatcg accgaacagg caaaccgcca aggcgtgtgg acggtgtcgg
      361 cggacaggtg ggcgagttcg ggaatgtgcc gtttgataaa agtaccgtcc gggtcggtac
      421 ggtatgaggc ggcggcaatg tcggggcagg tgtgccgtga ggcggcaagc caccagttgc
      481 cttggttgat tgccgcatcg aaatcggtca gctgccgggc aaaccatatc tcgccttcgc
      541 ggcgggggag gtttaaaacg tggcagaaaa aatccgcgct caagcgtctc agggcggggt
      601 ggaggctgcc ggttttgtgc aaacagcgca tcgcggcatc gataatcgga atgccggtct
      661 ggccctgctg ccaaagcgtc aggcgcaggg tgtgttcagg attgccgtct gaagggtcgt
      721 catccgtgtg ctgcaaggca agttgaagga aaaaatcgcg gcggatgatg ttgtccgccc
      781 acgcgttcag acggcgttcg aggctttccc gcgcgagcag gcgcggcgag atgcagccgg
      841 cactcaaata cgcgcccatc agcgaagtgt gtttgcgcga ggggaaatcc tttaaaacgg
      901 agtaggaatc cgcctgttcg agaaaccgcc gccactgccg ccaagccgcc gtttcgccgc
      961 tgttttgcgg caggaagatg ccgtctgaaa gggcggcagg cggcggggcg gaaaggtttt
     1021 cggggaaggg ttggcggtat gccgcgaata ggtccggacc ggcggggggc tgcttggaaa
     1081 agcggtcgag ccatacttcg cggtagcggt cgaaatcggt gtgtgccgtg ccgccgtcgg
     1141 gtatcaggtc ggttttgccg aaaacggcgc ggtcattgac gaaggttaac gcgatgccgt
     1201 gtttgttcaa ttcatgccaa agggcgttgt cggcgagttt gtcggcaaaa gtatgggatt
     1261 cgtcggcgat gacggcgcgg atattgaggc ggacggcgag ccggacgagc tcggcaggcg
     1321 atgccgccgt gtagagcggg atgccgcgcc ctgcaagccc ttgggcgagt tcggcggcgg
     1381 attggcggta gaacgcggcg cggcgagggt tgtctgtttc ggcatcgtca atccaaatgc
     1441 cgataatggg caaaccttcg gcaacggcgg cgcataaggc ggcgttgtcg cggatgcgga
     1501 ggttttggcg gaaccagacg agcgtgtgtg cggcgcacgt gtccgcataa agggggcggg
     1561 cggtttcaga cggcatttcg gcagcctttc ctgctggcga ttttttcgtt cagaaaatcg
     1621 atgaagctgc ggactttcgc gcttaagaat gccctgtctg cataaacggc attcagccgg
     1681 tcggtcggga cggcgtatcc aggcagcagc ctcaccagcg tgccgcagcg caaatcgtgt
     1741 tccgccgccc aaagcggctg ataaccgatg cacgcgcccg ccttaatcat ttcgcgcatc
     1801 atcagcgtgt tgtcggtacg gatgacgggg gtcagttcaa gccggtattt tttgccgtcc
     1861 gatttgcggg tgaggtcgag tttctgctgg ttggtgtagg tcggcaggac ggcgggcagc
     1921 cccgccactt cttccggcgt ttccggcacg ccgttgcgcc tcaggaaatc gggcgaggcg
     1981 agcagggcaa attcgatttc cgccagtggg cgcgcaatca gcgacgggga cagggtttgg
     2041 gaaacgcgca acgccaaatc cacgccttcg gcaatcaaat cgacgtggcg gttgtccaaa
     2101 atcagttcta atgccacttc gggataacgt tcgcggtatt ccgccagcca gttgcatatc
     2161 tggctgccgg caaaccacag cggcatcgtt acgcgcagca gcccctgcgg tttttccgtc
     2221 cctccggcgg ctttttgcgc ggcatcgtcg agcgtgtcga gcgcgtaact gcattgccgg
     2281 tagtattctt ccccggctcc ggtcaggctg aggttgcggc tgttgcggtg caggagtttg
     2341 gcttggacgg tgttttccaa gtggctgacg tgtttgcttg ccattgcggt ggagatgccg
     2401 agcgcgtcgg cggcgcgggt gaagccgccg ctttggacga cttggcggaa aaccttgagg
     2461 ctgaacaggg tgtccatatt ttcttgtgtg gaaaagttgt atcaataaaa gcagtatata
     2521 tttgaaaagg ggaaacatct atactctacc gcctgaaatg aagacaaata tcaaaggagc
     2581 ttttatgtcc gattgctgca accgtatcca accggttttg ctttctgttt tgcgtatcgt
     2641 aaccgcctac ctgtttttgt tgcacggtac gtcgaaaatc ttcgccttcc ccattgaaat
     2701 gggcagcggt tcgcccggcg ggctgttgct gcttgccggt attttagaaa ttgtcggcgg
     2761 cattttgctg gtgttgggcc tgtttgcgcg ccctgccgcg tttgttttgt ccggccagat
     2821 ggcggttgcc tattttatgg cgcacgcttc cggaaatgct ttgttcccga ttgccaacgg
     2881 cggcgagtcc gcagtgctgt tctgcttcgt attcctctat atcgcggcgg cgggcggcgg
     2941 agcatggtcg ctggacaggc tgtttttcaa gcgtaaagcc tgaatcggac tgcctaaagt
     3001 gtattttgtt gaatgttttt gaggaaaaga aatgacccgt caatctctgc aacaggctgc
     3061 cgaaagccgc cgttccattt attcgttaaa taaaaatctg cccgtcggca aagatgaagt
     3121 tgtccaaatc gtcgaacacg ccgttttgca cacaccttct tcgttcaatt cccaatctgc
     3181 ccgcgtggtc gtgctgtttg gcgaagagca tgataaggtg tggcaatttg tcgaagacgc
     3241 gctgcgtgcc gccgtgcctg ccgacagttt tgaaccgacc gcgcaaaaat tgaacctgtt
     3301 taaggcgggt gcggcaacca ttttgtttta tgaagatcaa aatgtcgtca aaggtttgca
     3361 ggagcagttc cctgcttatg ccgccaactt tcccgtttgg gcggaccagg cgaacgcgat
     3421 ggtgcagtat gccgtttgga cgacacttgc cgcggtcggc gtaggtgcaa acctgcaaca
     3481 ttacaatccc ttgcccgatg cggcgattgc caaagcgtgg aatatccccg aaaactggtt
     3541 gttgcgcgcg caaatggtta tcggcggtat tgaaggggcg gcaggtgaaa agacctttga
     3601 accagttgca gaacgtttga aagtgttcgg cgcataattt cgcggtcaaa aaaatgccgt
     3661 ctgaaccctg ttcagacggc atttttcagt atcaggcggc gagttttcct cattctgaga
     3721 cctttgttta caaatatcat gttcaatata gttaaaagaa attattctca tttcctccgt
     3781 gaggcaatat aattcggttg ttttgttaaa ttgagtataa aaatgaaaat atcatttcat
     3841 ttagctttat tacccacgct gattattgct tccttccctg ttgctgccgc cgatacgcag
     3901 gacaatggtg aacattacac cgccacgcta cctaccgttt ccgtggtcgg acagtccgac
     3961 accagcgtac tcaaaggcta catcaactac gacgaagccg ccgttacccg caacggacag
     4021 ctcatcaaag aaacgccgca aaccatcgat acgctcaata tccagaaaaa caaaaattac
     4081 ggtacgaacg atttgagttc catcctcgaa ggcaatgccg gcatcgacgc tgcctacgat
     4141 atgcgcggtg aaagcatttt cctgcgcggt tttcaagccg acgcatccga tatttaccgc
     4201 gacggcgtgc gcgaaagcgg acaagtgcgc cgcagtactg ccaacatcga gcgcgtggaa
     4261 atcctgaaag gcccgtcttc cgtgctttac ggccgcacca acggcggcgg cgtcatcaac
     4321 atggtcagca aatacgccaa cttcaaacaa agccgcaaca tcggtgcggt ttacggttcg
     4381 tgggcaaacc gcagcctgaa tatggacatt aacgaagtgc tgaacaaaaa cgtcgccatc
     4441 cgtctcaccg gcgaagtcgg gcgcgccaat tcgttccgca gcggcataga cagcaaaaat
     4501 gtcatggttt cgcccagcat taccgtcaaa ctcgacaacg gcttgaagtg gacggggcaa
     4561 tacacctacg acaatgtgga gcgcacgccc gaccgcagtc cgaccaagtc cgtgtacgac
     4621 cgcttcggac tgccttaccg catggggttc gcccaccgga acgattttgt caaagacaag
     4681 ctgcaagttt ggcgttccga ccttgaatac gccttcaacg acaaatggcg tgcccaatgg
     4741 cagctcgccc accgcacggc ggcgcaggat tttgatcatt tctatgcagg cagcgaaaat
     4801 ggcaacttaa tcaaacgtaa ctacgcctgg cagtagactg acaacaaaac cctgtcgtcc
     4861 aatttcacgc taaacggcga ctacaccatc ggccgttttg aaaaccacct gaccgtaggc
     4921 atggattaca gccgcgaaca ccgcaacccg acattgggtt tcagcagcgc ctttactgct
     4981 tccatcgatc catacgaccg agcaagctgg ccggcttcgg gcagattgca gcctatcctc
     5041 acccaaaacc gccacaaagc cgactcctac ggcatctttg tgcaaaacat cttctccgcc
     5101 acgcccgatt tgaaattcgt cctcggcggc cgttacgaca aatacacctt taattccgaa
     5161 aacaaactca ccggcagcag ccgccagtac agcggccact cgttcagccc caacatcggc
     5221 gcagtgtgga acatcaaccc cgttcacaca ctttacgcct cgtataacaa aggtttcgcg
     5281 ccttatggcg gacgcggcgg ctatttgagc atcgatacgt catcttctgc cgtgttcaac
     5341 gctgaccccg agtacacccg ccaatacgaa accggcgtta aaagcagttg gctggacgac
     5401 cgcctcagca ccacattgtc cgcctaccaa atcgaacgct tcaatatccg ctaccgcccc
     5461 gacgcacaaa ataatcccta cacttgggca gtcggcggca aacaccgttc gcgtggcgtg
     5521 gaattgtccg ccatcgggca aatcatcccc aaaaaactct atctgcgcgg ttcgttgggc
     5581 gtgattcagg cgaaagtcgt tgaagacaaa gaaaatcccg accgagtggg catccatttg
     5641 aataacacca gcaacgttac cggcaacctg tttttccgtt ataccccgac cgaaaacctc
     5701 tacggcgaaa tcggcgtaac cggtacaggc aaacgctacg gttacaactc aagaaataaa
     5761 gaagtgacta cgcttccagg ctttgcccga gttgatgcca tgcttggctg gaaccataaa
     5821 aatgttaaca ttacctttgc cgcagccaat ctgctcaatc aaaaatattg gcgttcggac
     5881 tctatgccgg gtaatccgcg cggctatact gcccgggtaa attaccgttt ctgatgaaat
     5941 caggcaaagg ctgaaataaa actaaacaca ttttttcact caaatcgaac acgccttcaa
     6001 taaaatgcca taaaatccgc acattaatct gacacacaag agatacctat gaaactgaaa
     6061 accttagctt tgacttcatt gaccctgttg gcattggccg cttgtagcaa acaggctgaa
     6121 accagtgttc cggcagacag cgcccaaagc agctcatctg ctccggcagc ccctgctgag
     6181 ttgaacgaag gtgtgaacta cactgtattg tctacgccta ttccgcaaca gcaggccggt
     6241 aaaatcgaag tattggaatt tttcggctac ttctgcccgc attgcgccca tcttgagccg
     6301 gtcttgagcg agcacatcaa aacgtttaaa gacgatacct atatgcgccg ggagcatgtc
     6361 gtgtggggtg atgaaatgaa acctttggca cgtttggcgg ccgcagtgga aatggccggt
     6421 gaatcagata aagccaacag ccatattttc gatgcgatgg ttaatcaaaa aatcaatctg
     6481 gccgataccg ataccctgaa aaaatggctg tccgagcaaa cagcgtttga cggcaaaaaa
     6541 gtattggctg catttgaggc tcctgaaagc caagcgcgtg cggctcaaat ggaagagttg
     6601 accaataaat tccaaatcag cggcacaccg actgtgattg tcggcggcaa ataccaagtt
     6661 gaatttaaag actggcagtc cggtatgacc acgattgacc agttggtgga taaagtacgc
     6721 gaagagcaga aaaagccgca ataagttgag gattgaatga gtaaaggcca tctgaaaata
     6781 ggatttcaga cggccttttg tatttaggct ttatagaaga gatgattgct taaagcctta
     6841 tggttttaaa tcagaatata tag
//
LOCUS       DAXHRI010000108         6750 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXHRI010000108 DAXHRI010000000
VERSION     DAXHRI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.108"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(110..469)
                     /gene="rplT"
                     /locus_tag="ACFM75_001550"
     CDS             complement(110..469)
                     /gene="rplT"
                     /locus_tag="ACFM75_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC6256071.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(482..679)
                     /gene="rpmI"
                     /locus_tag="ACFM75_001551"
     CDS             complement(482..679)
                     /gene="rpmI"
                     /locus_tag="ACFM75_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC6256072.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(826..1347)
                     /gene="infC"
                     /locus_tag="ACFM75_001552"
     CDS             complement(826..1347)
                     /gene="infC"
                     /locus_tag="ACFM75_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC6256073.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="ACFM75_001553"
     CDS             complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="ACFM75_001553"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC6256074.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWENYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEQRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENYAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3332..3408)
                     /locus_tag="ACFM75_001554"
     tRNA            complement(3332..3408)
                     /locus_tag="ACFM75_001554"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3372..3374),aa:Val,seq:gac)
     gene            3587..4702
                     /gene="tgt"
                     /locus_tag="ACFM75_001555"
     CDS             3587..4702
                     /gene="tgt"
                     /locus_tag="ACFM75_001555"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC6256075.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQAQFHENRARGTD"
     gene            4712..5794
                     /gene="hemH"
                     /locus_tag="ACFM75_001556"
     CDS             4712..5794
                     /gene="hemH"
                     /locus_tag="ACFM75_001556"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC6256076.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETLEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            5985..6386
                     /locus_tag="ACFM75_001557"
     CDS             5985..6386
                     /locus_tag="ACFM75_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6256077.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAQGKKN"
     gene            complement(6449..6739)
                     /locus_tag="ACFM75_001558"
     CDS             complement(6449..6739)
                     /locus_tag="ACFM75_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256078.1"
                     /translation="MAKYFTETGIGVREHFDFFGEFVVSPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVPR"
BASE COUNT         1723 a         1830 c         1572 g         1625 t
ORIGIN      
        1 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
       61 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
      121 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
      181 cagccaatac tttgcggtcg atttcgatag aggcgcgttt cagaccgttc ataaatttgc
      241 tgtaagacaa cccgttttca cgcgcacctg cattgatacg gacaatccac aattgacgga
      301 attggcgttt gcgttggcgg cggtcgcggt acgcgtattg accggctttc attaccgcct
      361 gcttggcaac gcggtaaacg tttttacgac ggccgcggta gcctttggct aacgcgaaga
      421 ttttttggtg acgggcacgg gcggtaacac cgcgttttac gcgtggcata ttctaaactc
      481 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcgttt accatagagg
      541 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
      601 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
      661 tggttttcat tttaggcatg ggaaaactcc attcgttatc agataaggca ttagggggtt
      721 ttaaaacatc ggtttcaaac acttgaaccc acaataccac ggttttttgc cgcaattgaa
      781 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
      841 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
      901 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
      961 ttcacggccg cggaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     1021 gatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttgatttc
     1081 cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgtttcttgg cctgctggta
     1141 tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg gggaaatctc
     1201 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     1261 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     1321 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     1381 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     1441 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     1501 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     1561 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcccgacaa
     1621 taatctgcct gattttcggt aatattcata attaccaatt gaaccggagc cagccataac
     1681 gggaaagagc ctgcatagtt ctcaatcaga atgccgataa atcgctccaa agaacctaaa
     1741 atggcgcgat gcaacataac aggtcgcgca cggtcattgt tttcggttac atattctgca
     1801 tccaagcgtt ccggcaagac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     1861 aaggcatctc tgacatgata ttcgatttta ggcccgtaaa acgcaccctc gcccggcaat
     1921 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     1981 cacacgtcat ctgaacctgc acgttgttca gggcgaagag aaagcttgac ggatacatca
     2041 tggaaaccga actgtttgta gatgcgaacc aacaattcat tgaacgcacg ggcctcgctg
     2101 acgatttgat cttcggtaca gaagatatgc gcatcatctt gcacaaaacc gcgaacacgc
     2161 atcagaccat gcaatgcacc gcttggttca ttgcggtggc aagaaccgaa ttccgccaaa
     2221 cgcatcggca aatcgcgata tgagcgcaaa ccgttattaa aaatttgcac atgacccgga
     2281 cagttcatcg gtttaaccgc atattcgcgt ttttccgaac tggttacgaa catattgtct
     2341 ttatagtttt cccagtggcc ggatttttcc cagaaagttt tatccataat ctgaggcgtt
     2401 ttcacctctt tataaccggc agcattcaac tctttacgca tatgctgttc aatcacttgc
     2461 cacaaagccc agcctttagg atgccaaaac accatgcccg gtgcttcgtc ttgcaaatgg
     2521 aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     2581 tgaatatagg ctttcaactc gtcttttgtc gcccaagccg taccgtatat gcgttgcagc
     2641 atttcattgt tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     2701 ttcaggaaac gggtattcgg aacgtgcggt ccgcggcaca tatcgacata ttcctgatga
     2761 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     2821 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     2881 tcatagtctt gagcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     2941 gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct cttcaatgac ggggccgata
     3001 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg ggcgcaggaa
     3061 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaatct
     3121 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc caccgccgcc
     3181 ttcgccaaac cggcaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     3241 tggcggactg agccgtccgg caaggtaatg ttcaacatca aaaagctcca aaacattaaa
     3301 aaataacggc ataaatgccg ttatgggaat ttggtaggca cgattggatt cgaaccaacg
     3361 acccccacca tgtcaaggtg gtgctctaac caactgagct acgtgccttc aaagaatttt
     3421 tgcattttat cgggtcgttt taatttttgc aagaggtatg tgcgtttctt tatgcaacat
     3481 tgcggcaaga tttggggatg tcgtctgaaa aaccgtggga aatcatttat aatgccgtct
     3541 tttaaaacat atttcgcccc ctgtcgccca actgacattc aatcttatgc ttaaatttac
     3601 cttacacaaa aaagacggat acgcccgacg cggcacgctg gagttgaacc acggcaaaat
     3661 cgaaacgccg gtatttatgc ctgtcggtac ttacggctcg gttaaggcga tgaacccgca
     3721 aaacctgcac gacatcaagg cgcaaattat tttgggcaac acttatcatt tgtggctgcg
     3781 tccgggcttg gaggtcgtcg aacagtttgg agggcttcac ggctttatcg gctgggacaa
     3841 accgattttg accgactcgg gcggttttca ggttttttct ttgtcggata tgcgcaagct
     3901 gacggaagaa ggctgtacgt ttaaaagccc gattaacggc gacaagctgt ttttatcgcc
     3961 tgaaatttct atgaaaatcc aaacggtttt gaactcggac atcgcgatgc agttggacga
     4021 atgcacgccg ggcgaggcga cgcgcgaaca ggcgcgaaaa tcgctgcaaa tgagcctgcg
     4081 ctgggcggaa cggagcaaaa aagcctttga agatttgaaa aacccgaacg cgctgttcgg
     4141 catcgtgcaa ggcgcgatgt atgaggattt gcgcgaagaa tcgctgcgcg gtttggaaca
     4201 gtttgatttt cccggccttg ccgtcggcgg cttgtccgtc ggcgaaccca agcccgaaat
     4261 gtaccgtatg ctgcgcgccg tcggtccgat tctgccggaa cacaagccgc attacctgat
     4321 gggcgtaggc acgccggaag acctcgtgta cggcgtggca cacggcatcg atatgttcga
     4381 ctgcgtgatg cccacccgca atgcacgcaa cggctggctg ttcacccgtt tcggcgattt
     4441 gaaaatcaaa aacgccaaac acaagctcga caagcgtccg atagacgaaa gctgcacctg
     4501 ctacgcctgc caaaacttca gccgcgccta cctgcaccat ctgcaccgcg ccggcgaaat
     4561 cttgggcgca cagctcaaca ccatccacaa cctgcatttc taccaagtca ttatggcgga
     4621 aatgcgcgat gccatagaac aaggcaaatt tgccgactgg caggcacaat tccacgaaaa
     4681 ccgcgcacgc ggcacagact gattcccggc aatgccgtct gaagccgctt cagacggcat
     4741 tttgccgctt taccctaccc gcactatttt tcagaaaccg atcatgcttc cattctttcc
     4801 agaaccctcc ctttcctata cgcaacaaaa ccgtaccgcc gttttgctgt tgaacctcgg
     4861 cacgcccgac gcgccgaccg cgcaggcggt acgaccttat ctgaagtcct ttctgaccga
     4921 ccggcgcatc gtcgaactgc ccaaatggct gtggtatccc attctgcatg gtctggtact
     4981 gacgttgcgc cccaaaaaaa gcgcgcacgc ctatgaaaaa atctggttta aggaaggttc
     5041 gcccctagaa gtttacacag cacgccaagc tgccgcgctt gccgaacgta tgcccgacct
     5101 catcgtccgc cacgccatga cctacggcaa tccttcggtg gcagacgtat tgtcggaact
     5161 gaaggcgcaa ggcgcgggca gactgctggt catcccgatg tacccgcaat atgccgcttc
     5221 cagcagcgga gcggcagtag ataaggtgtg cgaacaatta ctgctgcaac ggaaccaaat
     5281 gagcgtccgc accgtttcgc gcttttatga cgataccggc tacattgatg cgatgaaaaa
     5341 ccacatcctc cgatattggg cggaacacgg acgtgggaaa aaactgatgt tgagttttca
     5401 cggcgtaccg cagaagcact acgacctcgg cgacccctat cccgacgagt gccgccacac
     5461 cgccaaactg cttgccgaag cactggaact gaccgaagac caatatgtcg tatcgttcca
     5521 aagccaattc ggcagggcaa aatgggttac accgagcacg caggatttgt tcggcaaact
     5581 gcccaaacag ggggtaaccg agctggacgt attctgcccg ggctttttgg cagactgttt
     5641 ggaaacactg gaagaaatcg ccctgatggg gcgggaacag ttttatgaag caggcggcaa
     5701 aagctaccgc tatatcccct gcctcaacga caaccccgac tggatagacg cactcgtcgc
     5761 acttgccgaa gaaaaccttg gcggctggcg ttgaaactgt ttgacccatg tctaaaatgc
     5821 cgtctgaacg ccgtgttcag acggcatttt cccgctactt tagcgtatga tggcatctat
     5881 gctggacaac acatcaagtg caaccttact accgattcgg caagaaaatc tttcacccgc
     5941 tacgcaaaat caacaacaag gtttcaaaca ttaaggaaac accaatgaac acaacccgac
     6001 tgccgaccgc cctcgtcttg ggctgcctct gcgccgccgc ttctgccgcc gacaacagca
     6061 tcatgacaaa agggcaaaaa gtgtacgaat ccaactgcgt cgcctgccac ggcaaaaagg
     6121 gcgaaggccg cggaaccatg tttccgccgc tctaccgctc cgacttcatc atgaaaaaac
     6181 cgcaggtgct gctgcacagc atggtcaaag gcatcaacgg tacaatcaaa gtcaacggca
     6241 aaacctacaa cggattcatg cccgccactg ccatcagcga tgcggacatt gccgccgtcg
     6301 ccacttatat catgaacgcc tttgacaacg gcggcggaag cgttaccgaa aaagacgtaa
     6361 aacaggcaca aggcaaaaaa aactaaacag acaaaatgcc gtctgaaacc ggcaatccgg
     6421 cttcagacgg cattcaaatc aaacctttct aacgcggtac tttcagcccc atagacccga
     6481 tattgccgcc accgtcaacc tccttcgcgg tcagcataaa gccgccgatg tcaatacgac
     6541 cgcactcgac cggctcctga ctgtcggaac gcttatcgaa aagctcggca aggctcaaac
     6601 cctcttcgcc cgcttccagc ctcaaaccgt aagcaagtgc caaatcaccc gaacgtgctg
     6661 ccggcgaaac gacaaactcg ccgaagaaat caaaatgctc acggacgccg atacccgtct
     6721 cggtaaagta tttcgccatt ttatagtgaa
//
LOCUS       DAXHRI010000109         6657 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXHRI010000109 DAXHRI010000000
VERSION     DAXHRI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.109"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            230..676
                     /gene="yciA"
                     /locus_tag="ACFM75_001559"
     CDS             230..676
                     /gene="yciA"
                     /locus_tag="ACFM75_001559"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC6256079.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(727..1548)
                     /locus_tag="ACFM75_001560"
     CDS             complement(727..1548)
                     /locus_tag="ACFM75_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6256080.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPNMVARGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1667..2125
                     /locus_tag="ACFM75_001561"
     CDS             1667..2125
                     /locus_tag="ACFM75_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6256081.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2289..3404)
                     /gene="lst"
                     /locus_tag="ACFM75_001562"
     CDS             complement(2289..3404)
                     /gene="lst"
                     /locus_tag="ACFM75_001562"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC6256082.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3632..3772
                     /locus_tag="ACFM75_001563"
     CDS             3632..3772
                     /locus_tag="ACFM75_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HFC6256083.1"
                     /translation="MPPHIRHSTSDTAAKSGKTISPFLSRRRVQTRQDRTKREQSRQN
                     MP"
     gene            3839..6064
                     /locus_tag="ACFM75_001564"
     CDS             3839..6064
                     /locus_tag="ACFM75_001564"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC6256084.1"
                     /translation="MTQKSTIIYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPKYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELRAPVALK
                     AGEITDATVMSKKALLAFLAEQVQDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRILAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     TKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
     gene            6207..6515
                     /locus_tag="ACFM75_001565"
     CDS             6207..6515
                     /locus_tag="ACFM75_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256085.1"
                     /translation="MKTVPTILISILLATALPASAHGMHKSKPLTMDELPPICQQYFK
                     RAETCYNKTGDKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRISNSNAV
                     "
     gene            6505..6657
                     /locus_tag="ACFM75_001566"
     CDS             6505..6657
                     /locus_tag="ACFM75_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256086.1"
                     /translation="MPSETMISDGILTSKRTSLIKPAFPASLTRTGLNEVTALTIMAS
                     SHSQKL"
BASE COUNT         1800 a         1949 c         1469 g         1439 t
ORIGIN      
        1 cctgcgcggg aatgacggtt cagttgctta gggtaggatt tggcgggatt ggtgggctga
       61 agcccaccct acggccccgc cctacgcatc cgccctacaa tgcgccttgc gaatctgccc
      121 tacgcccgcc cttgtatgtc tcaaatgggt taaactacgc acttcccgac ttcccgtttc
      181 gggcagaggt gcaggttcga tttatatttc aataaaacaa ggaaacttta tgcagcacga
      241 agaaggcaac cgccaacgcc ctcaaggcga actgctcctg cgtaccgtcg ctatgccgcg
      301 cgataccaat cccaaccaag acattttcgg cggctggatt atgtcgcaga tggatttggg
      361 cggcggcata ttggcggcgg aaatcgcgcg gggacgcatc gttaccgtcg ccgttcagga
      421 aatgaacttt atccgcccgg tcaaggtcgg caacgtcgtc tgctgctacg ggcattgcgt
      481 ccgcgtgggc aatacttccc tccagcttaa aatcgaagtc tgggtgaaaa ctttgatgaa
      541 cgattgcgtt accgaagacc gctacctcgt aaccgaggcg gtgttcactt atgttgccat
      601 cgatgcggaa ggcaatccgc gcccgattcc gaaagaaggc aaccccaaat tgtcgggctt
      661 attgcctact ccgtaaaaat acccgtaaaa atgccgtctg aaactgtctt cagacggcat
      721 tccgcatcat ttgaaccagg acctgatgcg ggaaaacagc gacatactct gtttttcaaa
      781 gtcttcccgc acttcgggca cggctttttt tatcactttc attcctgcaa aagtctgcgc
      841 cacgggcata atttcaatcg tgttcacatt catatgcgcc ggccggcggt acagccacaa
      901 tgcggtttcc gcaatatctt cggggcggat aaattccaca ccctcataca cgcctgctgc
      961 cttctcgtca tcgcctttga agcgcacgtt ggaaaactcc gtattgccgc acaaacccgg
     1021 ctcgatattg gtaacgcgga tgttcttatc cgccaactcc gcgcgcaaat tcaggctgaa
     1081 ctggcgcaca aacgccttgg tcgccccgta aacgttgctg ccggcataag cataattacc
     1141 cgcaatcgaa cccaaattca tcacatagcc gccgccccga gccaccatat tcggcaaaat
     1201 cttgcgcgtt agaaacgtca aacccaaaac attggtttga atcattgttt cccaatcttc
     1261 aaaatccgcc ttgtccgccg tgtccagacc caaagccagc ccggcattgt tgatgaggca
     1321 gtcgatgtcg gaaaattcat cagggatgcc gtttaaggcg ttttccaccg actcgcggcg
     1381 cgacacgtcc atttccaaag ggtaaaacaa agcacccaat tcatccgcca aggcctgaag
     1441 cctgtccgca cgacgcgccg caccgataac gcggtatccc gccccgacaa acgcgcggca
     1501 catcgcttcg ccgaatcctg ccgaagcacc ggtaattaaa acagccattg tttttccttt
     1561 ctttctcgtt tcccaaacct gtttcaggta tcataatacc cttcagacgg catcgcgccg
     1621 gctgaaaatg acaacaacca taccaccacg acaggacaca ttcaatatga aaacccaaat
     1681 cagccttgct gctgccgcca tcacactgct cctttccgcc tgcggaggca gcggaatacc
     1741 ttcccaaccc aaaggcgaaa tttccgaaaa ccgcaccgcc gcgttcaaat ctatgatgcc
     1801 cgacttctcg cgtatgggca aaatggtcaa aggcgaagaa ccttacgatg tcgaaaaatt
     1861 caaacaggcg gcagcggcgt ttgccgacag cagcaaaaaa ccgtttacgc tttttgagtc
     1921 cgacccgcaa ggcaacgggc gcgcactgcc cgccgtttgg tcggatggtg caaaatttga
     1981 agccgaaaaa acaaaattcg ccgccgccgt cgaaaaactc aacgccgcag cacaaaccgg
     2041 caaactggat gaaatcaaag ccgcctacgg cgaaaccggc gcaagctgca aatcctgcca
     2101 cgacagtttc cgcgcgcccg aataacatcc aaaacaaaac aatgccgtct gaagccttca
     2161 gacggcattt tctttgccat ttcaccaagc gcaaagcaat caaaaattat cgttaattca
     2221 aaaaatacgc catatcctga tttttgcctt acttactccg ccattttcta ttttgatttg
     2281 ctatgcgatt aatctttatc gtcaaatgtc aaaatcggga tgccggattg ggtaaacagg
     2341 gcatacaccg gcttgagcca ataatcttcc ggaagggaag ccggtttcaa tgcgtaaacg
     2401 tgcacattgg gaaaatcctt catcgtcaac gccgcgccgc tgaaaaaggt ataaatttca
     2461 tacctcgtat gcgggttttt cttaatctcg cgcaaaatat agtcttcgat gacatagggc
     2521 gaatttaatg tggttacgcc ggaaagcccg taggtttggc gggggtgcgg tgcgacatat
     2581 tgtatgttga agttttttgc cgccttttcc gaaatttcct tcatctcctt gtcgggcgaa
     2641 cccaaaagta tccgcaccgt gccgcccgtt tcgtcccccg ccttcagttc ggacgcatcg
     2701 aacagcggca ggtaagtcat cttgcggcgg ccgtcgtcca taatgttttt caaacccttg
     2761 aatatcgtgt aatgctcgtc ggaagcattg cgggttttgg cgatgctcca atctccgatc
     2821 atcatccggg caaaattccg cttgatcgtc ccgtttacag aaaactcatc gcccaaatag
     2881 ctgctgcttt gaattaaatt gattgtcccg tcgtcaaagg ttttgatttc cgcatccggg
     2941 taagtgctca aaaaggcggc aatgctgact ttttccaaac ttgccaaata aatccgcttg
     3001 actttcggca gcagcatcga ctttaccttc agctccgcca tcgtcggaat gaaattaaac
     3061 gatttgttca aaccgtaggg caggtggaaa aaatacgccc gctccgcctt atcctttatc
     3121 tgattgaaat aataatcgta tttttcattc ctgttttcag acatcagcac cacataaaac
     3181 cgctcccccg gatgctgcgc cataatcctt tctgccacct tcatctgcaa tatggtatag
     3241 cagaaaatca gattgaccgg ttccccctct tcattgaaga gtttgtcctt cagcagggaa
     3301 accgcattcc tttccccctg atttacccgg tcaaatgtat aaaatatccc gaaacaaaaa
     3361 acaatcaggc acaacacggt caaacaagcc tttttcaagc ccatatccct aaaactccat
     3421 tccgacaaat tgaacatacg ccccgagtgt cccgggacgg aaaaatgcgg aattttagca
     3481 gatattccga cagttaccga atattatagt gatttaaatt taaatcagga caaggcgacg
     3541 aagccgcaga cagtacaagc agtacgacaa ggcgaggcaa cgctgtactg gtttaaattt
     3601 aaaccactat aaaatcaatt tcaccgaaaa tatgccgccg cacatcaggc acagcacctc
     3661 cgacaccgcc gccaaaagcg ggaaaaccat ttcccccttc ctttcacgtc gccgcgttca
     3721 aacacgccaa gatcgaacaa agcgcgaaca aagccgccaa aatatgccat aatagccacg
     3781 cacattcccc gcgcaatgtg ttccaaaaga aaaaaccaac ctacaagaga ataatcctat
     3841 gactcaaaaa tccaccatta tttataccca taccgacgaa gcccccgcgc tggcgaccca
     3901 atcgctgctg ccgattgtgc aggcttttgc ccgccacgcc gatattgatg tcaaaactgc
     3961 cgacatttcc ctgtccggcc gtattttggc ggcatttccc aaatatctga ccgaagcgca
     4021 gcgcgtacct gatgcgcttg ccgaattggg cgaactggtg aaacaacccg atgcaaacgt
     4081 aatcaaactg ccgaacatca gcgcatccgt accgcaactg actgccgcga ttaaagaatt
     4141 gcagtctaaa ggctttgccg ttcccgacta tcccgccgac ccgcaaaccg atgaagaaaa
     4201 agccgtgcgc gaacgctacg accgcatcaa aggcagcgcg gtaaaccctg tcctgcgcga
     4261 aggcaactcc gaccgccgtg cgcctaaagc agtgaaaaac tttgccaaaa aaaatccgca
     4321 cagcatgggc gcatggacga aagactccaa aacccacgtt gccaccatgc aaagcggcga
     4381 ctttttccat aacgaacaat ctgttactgt acccgatgcg acttccgtat ccatcgtgtt
     4441 caccgacaaa caaggcaaca aaaaagagct gcgcgcgcct gttgccctga aagccggcga
     4501 aatcaccgac gcgaccgtga tgagcaaaaa agccctgctc gccttccttg ccgaacaagt
     4561 gcaagacgcg aaagctaaag gcgtattgtt ctcgctgcat atgaaagcca cgatgatgaa
     4621 agtgtccgac ccgattatct tcggacacgc cgtcaaagta ttcttcgcgc ctgtatttga
     4681 aaaattcggc ggcaaactgg ccgccgcagg cgtcaacgtc aacaacggtt tcggcaacct
     4741 gcttgccaat ctggacaaac tggatgcgga cacccgcacc gccgtcgaag ccgaaatcgc
     4801 cgccgtttac gccgccaacc ccgatttggc gatggtcgat tccgataaag gcattaccaa
     4861 cctgcacgtt cccagcgatg tcatcgttga tgcttccatg cctgcgatga ttcgtaactc
     4921 cggccgtatg tgggataaag acggcaaagc gcaagacacc aaagccgtga ttcccgacag
     4981 cagctacgcc ggcgtttacc aagcaaccat cgacttctgc cgcgaacacg gcgcatttga
     5041 cccgacaacc atgggtactg tgcccaacgt cggactgatg gcgcaagcgg cggaagaata
     5101 cggctcgcac aacaaaacct ttgaaatcga agccgacggc caagtccaag tcattgatgc
     5161 ggcaggaaaa gtcctaatgc agcacgacgt tgaagccggc gacatctggc gtatgtgcca
     5221 aaccaaagac gctccggtta aagactgggt acaacttgcc gtcaaccgcg cccgtctgag
     5281 caacacgcct gccgtgttct ggctcgacga aaaccgtccg cacgacaaga gcctgctcgc
     5341 caaggttaaa gcctaccttg ccgaactgga taccaatggc ctcgacatcc gcatcctcgc
     5401 tcctgaagaa gccgccaagt tcagcttggg tcgtctgaaa aacggcgaag acaccatctc
     5461 cgtaaccggt aatgtcttgc gcgactacct gaccgacttg ttcccaattt tggaactcgg
     5521 cacaagcgcg aaaatgctgt ctatcgttcc attgatgaac ggcggcggta tgtttgaaac
     5581 cggcgcgggc ggttctgcac cgaaacatgt tcaacaattc ctcgaagaaa accatttgcg
     5641 ctgggactcg ctgggcgaat ttctcgcact cgccgtatcg tttgaacatc tggcgcaaaa
     5701 aaccggcaac accaaagccc aagtcctcgc cgacactttg gatgcagcca ccgaaaaact
     5761 gctgttgaac gacaaatcgc ccaaacgcaa agcgggcgaa ctcgacaacc gcggcagcca
     5821 tttctacctc accctctact gggcgcaaga attggcggcg caagacaaag atgccgaact
     5881 gaaagccgca tttacgccat tggcagccgc tttgaccgcc gacgaagcga aaatcgttgc
     5941 cgagctttcc gccgtacaag gcaaagcggc cgacatcggc ggctactacg ccgccaatcc
     6001 tgaaaaagcc gcacaagtga tgcgtccgag cgtaaccttt aatcaggcac taaacgcctt
     6061 ataagcacaa aggtaaaaat gccgtctgaa cattcgtcgt tcagacggca ttagttatcc
     6121 ctatctgcct gattatgatt aaacgccgca cccattggat ataaatcata taatctttag
     6181 ttttgaccct acggaaagac aataatatga aaactgttcc aaccatcctg attagcatcc
     6241 ttttggcaac cgcccttccc gcatccgcac acggtatgca taagagcaaa cccctgacta
     6301 tggacgagct gccgccgatt tgccaacaat atttcaaacg cgccgaaacc tgttacaaca
     6361 aaaccggaga caaagcagat ttcgcacgca acaacaccaa attcctattc caagccctgc
     6421 ctgctgccga tttgggacag cgtaagcaaa tgtgccaaat cgctatggat tcgtttgcag
     6481 aaaaaacccg catcagcaat agcaatgccg tctgaaacaa tgatttcaga cggcattctc
     6541 acatcaaaac gcactagctt gataaaaccc gccttccccg catccctgac ccgaacagga
     6601 ttaaacgaag taacggcttt aaccattatg gcaagttccc attctcaaaa attatag
//
LOCUS       DAXHRI010000110         6655 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXHRI010000110 DAXHRI010000000
VERSION     DAXHRI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.110"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            71..997
                     /gene="fabD"
                     /locus_tag="ACFM75_001567"
     CDS             71..997
                     /gene="fabD"
                     /locus_tag="ACFM75_001567"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC6256087.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAGHAVVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LIFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKAICAEAAQSEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIETH"
     gene            1136..3001
                     /gene="msbA"
                     /locus_tag="ACFM75_001568"
     CDS             1136..3001
                     /gene="msbA"
                     /locus_tag="ACFM75_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC6256088.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDSIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3101..4666
                     /gene="guaA"
                     /locus_tag="ACFM75_001569"
     CDS             3101..4666
                     /gene="guaA"
                     /locus_tag="ACFM75_001569"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC6256089.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIVGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4728..5474
                     /gene="fabG"
                     /locus_tag="ACFM75_001570"
     CDS             4728..5474
                     /gene="fabG"
                     /locus_tag="ACFM75_001570"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6256090.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            complement(5738..6001)
                     /locus_tag="ACFM75_001571"
                     /pseudo
     CDS             complement(5738..6001)
                     /locus_tag="ACFM75_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6038..6529)
                     /locus_tag="ACFM75_001572"
     CDS             complement(6038..6529)
                     /locus_tag="ACFM75_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6256091.1"
                     /translation="MKYRRFYRNGGTYFFTVATNKRQRILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSKYLIGGNLRLWQKRFWEH
                     TIRDEADFACHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT         1629 a         2090 c         1605 g         1331 t
ORIGIN      
        1 tttgtcccgc ctgaattttt cgtaaaactc gggcagaata cctgattatc caaccaaata
       61 aaggaatact atgtcttttg ccttcttttt tcccggacaa ggttcccaaa gcctcggtat
      121 gatgaacggc tttgccggac acgccgtcgt taaaaacacc tttgccgaag cctccgccat
      181 attggggcag gacttgtggg cgatgataaa cggcagcgat gccgaaatca tcggtcaaac
      241 cgtcaacacc cagcccatta tgctcgccgc cggcgttgcc gtttaccgcg cctatttaga
      301 agcgggcggc aaaacgcctg ccgccgttgc cggacacagc ctcggcgaat acaccgcact
      361 cgttgccgcc ggcgcattga tttttgccga cgcggtcaaa ctcgtgcgcc tgcgcgccga
      421 actgatgcag tccgccgtac cgcaaggcgt gggcgcaatg gcggcgattc tcggcttgga
      481 agatgagcag gttaaagcca tttgtgccga agccgcccaa agcgaagtgg tcgaagccgt
      541 caacttcaac tcacccggac aaatcgtgat tgcaggcaac gccgccgccg tcggacgcgc
      601 catggctgcc gccaaagaag ccggtgccaa acgcgccctg ccgctgcccg tgtccgtacc
      661 ttcccattgc agcctgatga aacccgccgc cgacaaactt gccgaagccc tgaaaaccgt
      721 tgaaatcaag cagccgcaaa tccgcgttat ccacaacgcc gacgttgccg cctacgatga
      781 tgccgacaaa atcaaagacg cgctcgtccg ccagctttac agccccgtac gctggacgga
      841 aaccgtcaac gccctcgttt cagacggcat tgccgaatcc gccgaatgcg gcccgggcaa
      901 agtgttggcg ggcttggcaa aacgcatcaa caaagccgcc gcgtgcagcg cactgaccga
      961 tgccggacag gttgccgcct ttatcgaaac gcactgactt cgttccgcaa aaagcagcct
     1021 gcatgcctca ggctgctttt cacgtccgaa cggcaaacag ccgcatattt gcgctataat
     1081 tgatgacgac caaaccaccg acagcggctg ccgttgcagt tcccgcccta ccgatatgat
     1141 agaaaaactg actttcggac tgtttaaaaa agaagacgcg cgcagcttta tgcgcctgat
     1201 ggcgtacgtc cgcccctaca aaatccgcat cgttgccgcc ctgattgcca ttttcggcgt
     1261 tgccgccacc gaaagctacc ttgccgcctt catcgccccc ctgattaacc acggcttttc
     1321 cgcacctgcc gcgccgcccg agctgtctgc cgccgccggc atcatttcca ccctgcaaaa
     1381 ctggcgcgaa cagtttacct atatggtttg ggggacggaa aacaaaatct ggaccgtccc
     1441 gctcttcctc atcatcctcg tcgtcatccg tggcatctgc cgctttacca gcacctatct
     1501 gatgacttgg gtctccgtga tgaccatcag caaaatccgc aaagatatgt ttgccaaaat
     1561 gctgaccctt tcctcccgct accatcagga aacgccgtcc ggcaccgtac tgatgaatat
     1621 gctcaacctg accgaacagt cggtcagcaa cgccagcgac atcttcaccg tcctcacgcg
     1681 cgacacgatg atcgttaccg gcctgaccat cgtcctgctt tacctcaact ggcagctcag
     1741 cctcatcgtc gtcctgatgt tccccctgct ctccctgctc tcgcgctact accgcgaccg
     1801 tctgaaacac gtcatttccg actcgcaaaa aagcataggc acgatgaaca acgtgattgc
     1861 cgaaacccat cagggacacc gcgtcgtcaa gctgttcaac gggcaggcgc aggcggcaaa
     1921 ccggttcgac gcggtcaacc gcaccatcgt ccgcctcagc aaaaaaatca cgcaggcaac
     1981 ggcggcacat tccccgttca gcgaactgat cgcctcgatc gccctcgccg tcgtcatctt
     2041 catcgccctg tggcaaagcc aaaacggcta caccaccatc ggcgaattta tggcattcat
     2101 cgtcgcgatg ctgcaaatgt acgcccccat caaaagcctt gccaacatca gcatccctat
     2161 gcagacgatg ttcctcgccg ccgacggtgt atgtgcattt ctcgacaccc cgcccgaaca
     2221 ggacaaaggc acgctcgcgc cgcagcgtgt cgaagggcgc atcagcttcc gcaacgtcga
     2281 tgtcgaatac cgttcagacg gcatcaaagc cctcgacggc ttcaacctcg acatcagaca
     2341 aggcgaacgc gtcgccctgg tcggacgttc cggcagcggc aaatccaccg tcgtcaacct
     2401 gctgccccgc tttgtcgaac cgtctgccgg caacatctgc atagacagta tcgacatcgc
     2461 cgacatcaaa ctcgactgcc tgcgcgccca attcgccctc gtctcccaag acgtattcct
     2521 gtttgacgat accctttttg aaaacgtccg atacagccgt cccgacgcag gcgaagccga
     2581 agtcctgtcc gccctccaag ccgccaacct gcaaagcctg attgacagct ccccgctcgg
     2641 actgcaccag cccatcggat cgaacggcag caacttatcc ggcggacagc ggcaacgcgt
     2701 cgccattgcc cgcgccattt tgaaagacgc gccgatatta ttattggacg aagccaccag
     2761 cgcattagac aacgaatccg aacgcctcgt ccaacaagcg ctcgaacgcc tgatggaaaa
     2821 ccgcaccggc atcatcgtcg cccaccgcct gaccaccatc gaaggggccg accgcatcat
     2881 cgtgatggac gacggcaaaa tcatcgaaca aggcacacac gaacaactga tgtcccaaaa
     2941 cggctactac acaatgttac gcaatatctc aaacaaagat gccgccgtcc ggacggcata
     3001 aacaaaatgc cgtccgaaat ggtacaatcg ccccgaccct ttcagacggc atcatatccg
     3061 ccgacccatc cgattatctt caatcactgt aaaacccatt atgacccaag acaaaatcct
     3121 catcctcgac ttcggttcgc aagttaccca gctcatcgcc cgccgcgtgc gcgaagccca
     3181 cgtttactgc gaactgcatt ccttcgatat gcctttggac gaaatcaaag ccttcaaccc
     3241 caaaggcatc atcctctccg gcggccccaa ttccgtttac gaatccgact atcaagccga
     3301 taccggtatt tttgatttgg gcattccggt tttgggcatc tgttacggca tgcagtttat
     3361 ggcgcaccac ttgggcggcg aagtgcagcc cggcaaccag cgcgaattcg gttatgcgca
     3421 agttaaaacc atagacagcg agctgacacg cggcattcaa gatggtgagc caaacacact
     3481 cgacgtatgg atgagccacg gcgacaaagt gtccaaactg cccgacggtt tcgccgtcat
     3541 cggcaacacc ccgtcctgcc cgattgccat gatggaaaac gccgaaaaac aattctacgg
     3601 catccagttc caccccgaag ttacccacac caaacaaggc cgcgccctgt tgaaccgctt
     3661 tgtcttggat atttgcggcg cacaaccggg ctggacgatg ccgaactaca tcgaagaagc
     3721 cgttgccaaa atccgcgaac aggtcggcag cgacgaagtg attttaggtt tgtcaggcgg
     3781 cgtagactct tccgtagccg ccgcgctgat tcaccgcgcc atcggagacc aactgacctg
     3841 cgtgtttgtc gatcacggtt tgttgcgcct gaacgaaggc aaaatggtga tggatatgtt
     3901 cgcccgcaac ttgggtgtga aagtgattca cgtcgatgcc gaagggcagt ttatggcgaa
     3961 actcgccggc gtaaccgacc ccgagaaaaa acgcaaaatc gtcggcgcgg aatttatcga
     4021 agtatttgat gccgaagaga aaaaactcac caacgccaaa tggctggcgc aaggtacgat
     4081 ttaccctgac gtaatcgaat ccgcaggcgc gaaaaccaaa aaagcccacg ccatcaaatc
     4141 gcaccacaac gtcggcggtc tgcccgaaaa tatgaagctc aagctgcttg agccgttgcg
     4201 cgacttgttt aaagacgaag tgcgcgaact gggcgttgct ttgggtctgc cgcgcgaaat
     4261 ggtgtaccgc catcccttcc ccggccccgg tttgggcgtg cgcatcttgg gcgaagtgaa
     4321 aaaagaatac gccgacttat tgcgtcaagc agacgatatt ttcatccaag aattacgcaa
     4381 caccaccgac gaaaacggca catcttggta cgacctgacc agccaagcct tcgccgtgtt
     4441 cctgcctgtg aaatccgtcg gcgtaatggg cgacggacgc acttacgact acgtcgtcgc
     4501 actgcgcgca gtcatcacca gcgacttcat gaccgcacac tgggcagaac tgccttactc
     4561 actgctcgga cgcgtgtcca accgcatcat caacgaagtc aaaggcatca accgcgtggt
     4621 gtacgatgtc agcggcaaac cgcctgccac catcgagtgg gaataaccag caaacagggc
     4681 tgcaccgtcc ggcgcagtct ttcgattatc ggaaaaaagg aaaaaatatg agcacacaag
     4741 atttaagcgg caaaatcgct ttggtaaccg gcgcatcgcg cggtatcggt gcagcaattg
     4801 ccgacacact ggcggcagcc ggtgccaaag tcatcggtac ggcgaccagt gagagcggtg
     4861 cggcggcgat tagcgagcgg ttggcgcaat ggggcggcga aggccgcgta ttaaattccg
     4921 ccgaacccga aaccgtcgaa aacctgattg ccgacatcga aaaaacgttc ggcaaactcg
     4981 atattctggt caacaacgcc ggcatcaccc gcgacaacct cctgatgcgc atgaaagaag
     5041 aagagtggga cgacatcatg caggtcaacc taaaatccgt cttccgcgcc tccaaagccg
     5101 tcttgcgcgg catgatgaag cagcgcgccg gccgtatcat caacatcaca tccgtcgtcg
     5161 gcgtgatggg caatgccggt caaaccaact atgccgcggc aaaagcaggc ttaatcggtt
     5221 tctccaaatc catggcgcgc gaagtcggca gccggggcat taccgtcaac tgcgtcgccc
     5281 ccggctttat cgataccgac atgacccgcg ccctgccgga agaaacccgc caaaccttta
     5341 ccgcccaaac cgccttgggc agattcggcg acgcgcaaga cattgccgat gcagtcttgt
     5401 tcttggcttc cgatcaggca aaatacatta ccggtcaaac gctgcacgtc aacggcggca
     5461 tgctgatgcc ctgacgggca gccgtaccga caatgccgtc tgaagccctt tcagacggca
     5521 tttgcattcc caaacaaaat gaacacacac cacaccctgc cctgcccctg cgattcaggc
     5581 acaagctgag gcctttgcaa aattcctttc cctcccgaca gccgaaaccc aaacacaggt
     5641 tttcggctgt tttcgcccca aatacctcct aattttaccc aaataccccc ttaatcctcc
     5701 ccggataccc gataatcagg catccggtcg ccttttgggc ggcagcgggc gcacttagcc
     5761 tgttggccgc tttcaaaagg ttcaaacaca tcgccttcag gtggctttgc gcactcactt
     5821 taatcagtcc gaaataggct gcccgcgcat agcggaactt acggtgcagc gtaccgaagc
     5881 tctgttcgac cacataacgg gtcttcgaca aatatcggtt acgtttggtt tgcacttccg
     5941 acagcggacg gttgcggcag gctttgcgca taatgccgtc ctgcagccga tgctctttca
     6001 gttttccgta ggtcggattc tcgaatccga cattacttca atcgtatcca atagaaaagt
     6061 ccgcattgcc gccaccccaa ttatgcggat aaataccctg tttgacataa cggtgaaacg
     6121 tagaaaaccc ccaatcggaa atttgtccta catagccatg tttgaccgga ttgaaatgca
     6181 gataatcaaa atggcaggca aaatcggcct catcgcggat agtatgttcc caaaagcgtt
     6241 tttgccaaag cctgagattg ccgccgatta aatatttgct gtgccgcttg atctgccgcc
     6301 agcgttccga ataagcagaa tcattgtccg gcagccgcca tatggtatgc agatggtcgg
     6361 gcatcaacac ccatgccaaa atttcaaacg gataccgttc gcgcacagcc attaccgcct
     6421 gtcgcaaagc caaacgcacc gcatcatcgg tcaaaatcct ctgccgttta ttggttgcaa
     6481 ccgtaaaaaa gtaagtgccg ccattgcggt aaaaacgacg gtatttcata gtattatact
     6541 cggaatgatt gtataggtcg aattctcgaa tccgacattt cgggcattgc tgcaacggat
     6601 tgcaacgacg ggaatgttaa aggttttgtc ggatacgagt atccgaccta cgctt
//
LOCUS       DAXHRI010000111         6609 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXHRI010000111 DAXHRI010000000
VERSION     DAXHRI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.111"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            131..1036
                     /gene="glyQ"
                     /locus_tag="ACFM75_001573"
     CDS             131..1036
                     /gene="glyQ"
                     /locus_tag="ACFM75_001573"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC6256092.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1113..1523
                     /locus_tag="ACFM75_001574"
     CDS             1113..1523
                     /locus_tag="ACFM75_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HFC6256093.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLAANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLHATPLVDGFYGDDGWENYSETYKQAD"
     gene            1544..3607
                     /gene="glyS"
                     /locus_tag="ACFM75_001575"
     CDS             1544..3607
                     /gene="glyS"
                     /locus_tag="ACFM75_001575"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC6256094.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIINDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSGNQTLGHRFLSGGEIAIENADSYAAQMRGQGKVVA
                     SFAERKAAIQTALNEQAGRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDRNGKLMNRFLLVSNLQTEDPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDNLPNGKVA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEQTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMADDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3624..4670
                     /locus_tag="ACFM75_001576"
     CDS             3624..4670
                     /locus_tag="ACFM75_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6256095.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWCNLDILIVDDGSTDGTLAIAQRFQEQDGRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHEHG
                     KIWKKPIRHEDIAAFFPFGNPIHNNTMIMRRSVIEGGLRYDTECDWAEDYQFWYEVSK
                     QGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKDLSEEDFERVRRFLYQCFKRTDTPPAGAWLDFAADGRMRRLFTM
                     RQYFGILHRLLKNR"
     gene            4712..5539
                     /locus_tag="ACFM75_001577"
     CDS             4712..5539
                     /locus_tag="ACFM75_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6256096.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLKERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISK
                     KAIRFFLQHFVMLQPEQIKPIDLMMFSDFFDKEGMPVYQVSPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5551..5646
                     /locus_tag="ACFM75_001578"
                     /pseudo
     CDS             5551..5646
                     /locus_tag="ACFM75_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            5688..6530
                     /locus_tag="ACFM75_001579"
     CDS             5688..6530
                     /locus_tag="ACFM75_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6256097.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1710 a         1743 c         1733 g         1423 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatatca tgtatgccgt gttttcaaat cagtctgtct
       61 ttgagataaa cctatccaaa cagcaatgga aacagtaaaa taaacccttt attttatcga
      121 atcataagcc atgcttacct tccaacaaat catcttcaaa ctgcaaacct tctgggcaga
      181 caaaggctgc accgtcatcc aacccttcga catggaagtc ggtgccggca catcccatcc
      241 cgccacctgc ctgcgcgcgc tcgggcccga gccttggttt gccgcctacg tccaacccag
      301 ccgccgcccc aaagacggcc gctacggcga caaccccaac cgcctgcaac actattacca
      361 attccaagtc gccctcaagc ccgctcccgc taatatccaa gacctctatc tcgactctct
      421 gcgcgaattg ggcatcgatc ccaaagtcca cgacatccgc tttgtcgaag acgactggga
      481 aaaccccacc ctcggcgcgt ggggtttggg ctgggaagtc tggctcaacg gcatggaagt
      541 aacccagttt acctatttcc aacaagtcgg cggcatcgac tgcacgcccg tactcggcga
      601 aatcacctac ggcatcgaac gcttggcgat gtacttgcaa ggtgtagaaa acgtctacga
      661 cctcgtttgg gcaaaaacgc tcgacggcaa caccgtcacc tacggcgacg tgtaccacca
      721 aaacgaagtc gagcaatcca cctacaactt cgaatacagc gatgccgact ggctgctgcg
      781 ccagttcaac gactacgaag cgcaagccaa acgcctgctt gccgaagaaa acgccgccct
      841 cgccctgcct gcctacgagc ttgtcctcaa agcggggcat acgttcaacc ttttagacgc
      901 acgcggcgcg atttccgtaa ctgagcgggc aacttatatt ggacgtattc gtgcattgag
      961 ccgcgccgtg gcgcaaaaat atgttgaaag ccgcgaaaaa ctgggcttcc ctttaatgaa
     1021 ggcaaacgcc gcctaagggg gcgacgattt tttacgttcg ggcggcacga taaggcagcc
     1081 gtccggaagc cattatattt tttaggaaaa tcatgcctgt ctatttccgc accccaagca
     1141 ggaaacgcct gcacaaaatg aaccgccgcc agcataaaaa gctgcatttg ggcgagtttc
     1201 aaacattgat tttcggcgtt caaggcagtt tgcaccaagc tgaggacgat gccgaagcgt
     1261 tcgaccgctt tatggacgag ctgattgcgt atcttgcggc aaacggcttg gaaatggcgg
     1321 gcgggggcga ttgcggcgat ttttcgtgcc tgtttcaaaa atccggtgcc cgaagcttgg
     1381 gcgaaaccga gaaaaaagcc attatctctt ggctggcagg ccgcgaagat gtcgctactt
     1441 tgcacgccac cccgctggtt gatggttttt acggggatga cggctgggaa aattacagcg
     1501 aaacctataa acaggcagat taaccaaatt caaagaaagc cgaatgatga cccaaaccct
     1561 tttaatcgaa ctccttaccg aagaactccc gcccaaagcc ctgaataatc tgggcaacca
     1621 ttttgccgcc gccgttgccg aaggcttgga aaaagcgcaa ctggttgacg gtgcagccga
     1681 atttacggct tatgcctcgc cgcgccgttt ggcggttcaa gtcaaaaacg tcaaagccgt
     1741 tcaggccgac caaaaaatcg tgaaaaaagg ccctgccgtg gcgaatgccg taaaagacgg
     1801 tgcgccgacc aaggctttgg aaggtttcgc acgcggtgca ggggcgaaaa tcgaagacct
     1861 gaccatcatc aatgacggca agcaagacgt gtacgcctac gaatacgtcc aaaccggcaa
     1921 accattgggt gagcttttgg aagacatcat caatgccgca gtgaaaaaat tgccgattcc
     1981 taaagtcatg cgttggggca gcagcacatt taccttcgtg cgccccgtcc acgggctgat
     2041 cgtgctgcac ggcggcgaca tcgtaaacgt cagcgttttg ggtctgcaaa gcggcaatca
     2101 aacattgggt caccgcttcc tgtccggcgg cgaaattgcc attgaaaacg ccgacagcta
     2161 cgccgcacaa atgcgcgggc aaggcaaagt cgtcgcttcg tttgccgaac gcaaagccgc
     2221 cattcagacg gccttgaacg agcaggcagg ccgtctgaac gcgaccgttg ccgccgatga
     2281 agcattgttg gacgaagtga ccgcattggt cgaatggcct gtggtattgg aagccggttt
     2341 tgaagaacac ttcctcgccg taccgcaaga atgcttgatt ctgaccatgc agcaaaacca
     2401 aaaatacttc ccgctgctcg accgaaacgg caagctgatg aaccgcttcc tgctggtttc
     2461 caacctgcaa accgaagacc catcacacat catccaaggc aacgaacgcg ttttgcgcgc
     2521 gcgcctgtct gatgccgagt tcttctacaa acaagaccaa aaagcgactt tggaaagccg
     2581 cctgcccaaa ctggcaaacg tggtgtatca caacaaaatc ggttcgcaag ccgagcgtat
     2641 cgaacgcctg caaagcatcg ccgcccacat cgccaaagct ttgggcgcgg atgccgccgc
     2701 agccgaacgc gccgcgcgtt tggcgaaagc cgacttggtt accgaaatgg tcggcgagtt
     2761 ccccgaactg caaggcacga tgggcaaata ctatgcccgt ttggatggcg aaaccgaaga
     2821 aattgccgaa gccgtcgagc aacattacca accgcgtttt gccggcgaca acttgccgaa
     2881 cggcaaagtc gccactgccg ttgcacttgc cgacaagctg gaaaccctgg tcggcatttg
     2941 gggcatcggt ttgattccga ccggcgacaa agacccatac gccctgcgcc gcgccgcatt
     3001 gggtattttg cggatgctga tgcagtacgg tttggacgtg aacgagctga ttcagacggc
     3061 attcgacagc ttccccaaag gcttgctcaa cgagcaaacg ccgtctgaaa ccgccgactt
     3121 tatgcaggcg cgccttgccg tgttgctgca aaacgattat ccgcaagaca tcgttgccgc
     3181 cgtattggcg aaacagccgc gccgtttgga cgacgtagtg gcgaaactgc aagccgttgc
     3241 cgcgttcaaa caactgcccg aagccgccgc gctcgccgcc gccaacaaac gcgtgcaaaa
     3301 tctgctgaaa aaagccgatg ccgagttggg cgcggttaac gaaagcctgc tgcaacagga
     3361 cgaagaaaaa gccctctttg ccgccgcgca aggtttgcag ccgaaaatcg ccgccgccgt
     3421 tgccgaaggc aatttccaaa ccgccttgtc cgaactggct tccgtcaagc cgcaagtcga
     3481 tgccttcttt gacggcgtga tggtgatggc ggacgatgcc gccgtgaagc aaaaccgttt
     3541 gaatctgctg aaccgtttgg cagagcagat gaacgcggta gccgacattg cgcttttggg
     3601 cgagtaaccg ttgtacagtc caaatgccgt ctgaagcctt cagacggcat cgtgcctatc
     3661 gggagaataa attgcagcct ttagtcagcg tattgatttg cgcctacaac gtagaaaaat
     3721 atttcgccca atcattagcc gccgtcgtga atcaaacttg gtgcaacttg gatattttga
     3781 ttgtcgatga cggctcgacg gacggtacgc ttgccattgc ccagcgtttc caagaacagg
     3841 acggccgcat caaaatcctt gcacaagctc aaaattccgg tctgattcct tctttaaaca
     3901 tcgggctgga cgaattggca aagtcaggaa tgggggaata tattgcacgc accgatgcag
     3961 acgatattgc cgcccccgat tggattgaga aaatcgtggg cgagatggaa aaagaccgca
     4021 gcatcattgc gatgggcgcg tggctggaag ttttgtcgga agaaaaggac ggcaaccggc
     4081 tggcgcggca ccacgaacac ggcaaaattt ggaaaaagcc gatccggcac gaagacatcg
     4141 ccgccttttt cccttttggc aaccctatac acaacaacac gatgattatg cggcgcagcg
     4201 tcattgaggg aggcttgcgt tacgatactg aatgcgattg ggcggaagat taccaatttt
     4261 ggtacgaagt cagcaaacag ggcaggctgg cttattatcc cgaagccttg gtcaaatacc
     4321 gccttcacgc caatcaggtt tcatccaaat acagcatccg ccaacacgaa atcgcgcaag
     4381 gcatccaaaa aaccgccaga aacgattttt tgcagtctat gggttttaaa acccggttcg
     4441 acagccttga ataccgccaa ataaaagcag tagcgtatga attgctggag aaggatttgt
     4501 cggaagaaga ttttgaacgc gtccgccggt ttttgtacca atgcttcaaa cggacggaca
     4561 cgccgcccgc cggtgcgtgg ctggatttcg cggcagacgg caggatgagg cggctgttta
     4621 ccatgaggca atacttcggc attttgcacc gattgctgaa aaaccgttga aaaacgccgt
     4681 tttatccaac agacaaaaaa caggataaat tatgcaaaac cacgttatca gcttagcttc
     4741 cgccgcagaa cgcagggcgc acattaccga taccttcggc gtgcgcggca tcccgtttca
     4801 gtttttcgac gcactgatgc cgtctgaaag gctggaacag gcaatggcgg aactcgtacc
     4861 cggtttgtcg gcgcacccct atttgagcgg agtggaaaaa gcctgcttta tgagccacgc
     4921 cgtattgtgg aaacaggcat tggacgaagg tctgccgtat atcgccgtat ttgaggacga
     4981 cgttttactc ggcgaaggcg cggagaaatt ccttgccgaa gacgcttggc taaaagaacg
     5041 ctttgacccc gattccgcct ttatcgtccg cttggaaacg atgtttatgc acgtcctgac
     5101 ctcgccctcc ggtgtggcgg actactgcgg gcgtgccttt ccgctgttgg aaagcgaaca
     5161 ctgggggacg gcgggctata tcatttccaa aaaagcgata cggtttttcc tgcaacattt
     5221 cgtaatgttg cagccggaac agataaaacc tatcgactta atgatgttca gtgatttttt
     5281 cgataaggag gggatgcctg tttatcaggt cagtccggcc ttatgcgccc aagagctgca
     5341 ttatgccaag tttcacgacc aaaacagcgc attgggcagc ctgatcgaac acgaccgcct
     5401 cctgaaccgc aaacagcaat ggcgcgattc ccccgccaac acattcaaac gccgcctgat
     5461 ccgcgccttg accaaaatca gcagggaaag ggaaaaacgc cggcaaaggc gcgaacagtt
     5521 cattgtgcct ttccaataaa aggaggagag atggacatcg tatttgcagc agacggcagg
     5581 atgcggcggc tgtttacctt gaggcaatac ttcggcattt tgcgccgatt gctgaaaaac
     5641 cgttgaaaaa cgccgcttta tccaacagac aaaaaacagg ataaattatg caaaaccatg
     5701 ttatcagctt agcttccgcc gcagaacgca gggcgcacat tgccgcaacc ttcggcgcgc
     5761 gcggcatccc gtttcagttt ttcgacgcac tgatgccgtc tgaagaactg aatcggatga
     5821 tggcggaatt ggtgccggga ttggcaaaac agcacctgtt gagcgaagtg gaaaaagcct
     5881 gctttatgag ccacgccgta ttgtggaaac aggcattgga tgaaggtctg ccctatgttg
     5941 ccgtatttga ggatgatgtc ctgcttggca aagacgcaga aaagttcctt gccgaagata
     6001 cttggttgga agagcgtttt gataaggatt ctgcctttat cgttcgtttg gaaacgatgt
     6061 ttgcgaaagt tattgtcaga ccggataaag tcctgaatta tgagaaccgg tcatttcctt
     6121 tgctggagag cgaacattgg gggacggctg gctatatcat ttcgcgtgag gcgatgcggt
     6181 ttttcttgga aaggtttgcc gttttgccgg cagagtggat taaagcggta gattggatga
     6241 tgtttactta tttctttgat aaggagggga tgcctgttta tcaggttaat cccgccttat
     6301 gtacccaaga attgcattat gccaagttcc tcagtaaaaa cagtatgttg ggtagcgatt
     6361 tggaaaaaga tagggaacaa gaaagaagac accgccgttc gttgaaggtg atgtttgact
     6421 tgaagcgtgc tttgggtaaa ttcggtagag aaaagaagaa aagaatggag cgtcaaaggc
     6481 aggcggagct tgagaaagct tacggcaggc gggtcatatc gttcaaatag tttgtgtaaa
     6541 atatagcgga ttaaaattag aaatggatac actgtcattc ccgcgaaagt gggaatccag
     6601 tccgttgag
//
LOCUS       DAXHRI010000112         6471 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXHRI010000112 DAXHRI010000000
VERSION     DAXHRI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.112"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            1..336
                     /locus_tag="ACFM75_001580"
                     /pseudo
     CDS             1..336
                     /locus_tag="ACFM75_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            368..484
                     /locus_tag="ACFM75_001581"
     CDS             368..484
                     /locus_tag="ACFM75_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6256098.1"
                     /translation="MPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYRK"
     gene            complement(644..1981)
                     /gene="ribBA"
                     /locus_tag="ACFM75_001582"
     CDS             complement(644..1981)
                     /gene="ribBA"
                     /locus_tag="ACFM75_001582"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC6256099.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            2089..2217
                     /locus_tag="ACFM75_001583"
     CDS             2089..2217
                     /locus_tag="ACFM75_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256100.1"
                     /translation="MPSEAGFVASDGIAAIPAGGAAVSSRPLPIRGLPEAYSEKNF"
     gene            complement(2196..3209)
                     /locus_tag="ACFM75_001584"
     CDS             complement(2196..3209)
                     /locus_tag="ACFM75_001584"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6256101.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     RGGILFDLQQLADKKVDLILHPSSFNYRDIPKGADFSDNDFVRHFETLVEGRYYIANA
                     WTKIVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGNSISH
                     NIKYKNFSDMLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSISVRLYFSKNII
                     VVYREYFSFKEKCRKIFGAKAIHPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKF
                     FSE"
     gene            complement(3365..3958)
                     /gene="ribA"
                     /locus_tag="ACFM75_001585"
     CDS             complement(3365..3958)
                     /gene="ribA"
                     /locus_tag="ACFM75_001585"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC6256102.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3951..4628)
                     /locus_tag="ACFM75_001586"
     CDS             complement(3951..4628)
                     /locus_tag="ACFM75_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256103.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMGMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPICTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            4782..4866
                     /locus_tag="ACFM75_001587"
     tRNA            4782..4866
                     /locus_tag="ACFM75_001587"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4816..4818,aa:Leu,seq:tag)
     gene            complement(4957..5145)
                     /locus_tag="ACFM75_001588"
     CDS             complement(4957..5145)
                     /locus_tag="ACFM75_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256104.1"
                     /translation="MADERKGFFDAIIKINIFFFPVETPALGAVNLIAGGSTRFRFGA
                     EQMAALYVPFCVLKHIGR"
     gene            5386..6420
                     /gene="purM"
                     /locus_tag="ACFM75_001589"
     CDS             5386..6420
                     /gene="purM"
                     /locus_tag="ACFM75_001589"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC6256105.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1600 a         1576 c         1691 g         1604 t
ORIGIN      
        1 tcgacgcatc gggcttgggc gaaaaaatta aagacggcaa aaaaaccgta ctttcccgcg
       61 aagaagaaca aaaaaatctg caataccttc acgcacaaac aagcagtgga agatttcagc
      121 gtggtgatcg gctacgatga aatcaaagcg aagaatcaca gcttgtcggc ggggcagtat
      181 ttcgaggtaa agattgatta tgtggatatt tccgccgacg aatttgcgca aaaaatggcg
      241 ggattttcag cggatttgga taaacttttc gccgaatctg ccgaattgga aaaagagatt
      301 aaggatagat tggcgatgtt gaaattcaat tcataaatac cgatgaatga aaccggtcaa
      361 acttaaaatg ccgtctgaaa gcctttcaga cggcattttg tttggtttgg aaaatactta
      421 tgccgttctt tccctgaaag agagaatcca aaaaaccaaa gccacaggaa tttatcggaa
      481 atgacaaaaa cccgacgaac cggattcccg cctgcgcggg aatgacgaat tagaagttac
      541 ccgaaatttg caaaaacaaa atccaaccca acagaccggg ttttcgtttg cacggaaata
      601 atgcaataaa taaagcaaat ataaagtatt tgaatttact atattatttt tccgcttctt
      661 caaagccgac gacttccaaa ccgaagccgg tcaggccggt gaaagatgag ggctgcccga
      721 ggacgcgcag ttttttgacg ttgaggccgg cgaggatttg cgcgcctatg ccgtagcttt
      781 tgctgtccca tttgtaggct tggtttgcgc ctttaggcag ggtgcggtcg agcagggatg
      841 cgccgtcttc ggtgcggtgc agaaggatga cgacgccgct ttctgcctgt tggacgcgct
      901 cgagtgcttt gggcaatgac cacgaatggc gcgggttggc ttggatgaag tccatcacgc
      961 tgaagggttc gtggacgcgg acgagggttt cggtgtcggc agagggcgta cctttgacga
     1021 gggcgaggtg ggtttcgccg gagagtttgt cgacgtaaac gtgttgttgg aactcgcccc
     1081 acggggtttg tacaggcgca ttgcccatgt cttcgagcag gctttcggta cggctgcggt
     1141 attcgatgag gtcggtaatg gtgccgattt tgagcttgtg ttcttcggcg aatttcatca
     1201 gttcgggcat acgcgccatc gtgccgtcgt cgttgatgat ttcgcaaata acggcggcag
     1261 gaatcagccc gttcatttgc gccaggtcga cgccggcttc ggtgtgtccg gcgcggacga
     1321 gtacgccgcc tttttgggcg cgaagcggaa agatatgacc gggttggacg atgtcttcgg
     1381 gtttggcggt cggggaaacg gcggtttgaa tagtcagggc gcggtcggcg gcggaaatgc
     1441 cggtggtaat gccgtgtgcg gcttcgatgg agacggtaaa gttggtgccg tattgcgcgc
     1501 cgtttttttg ggtcatcatc ggcagcccga gtttttcgac catttcgccg tccatcggca
     1561 ggcagaccaa gccgcgcgcg tgtttgatca tgaagttgat ggcttcgggc gtgacgaatt
     1621 gcgccgccat cagcaggtcg ccttcgtttt ctcggtcttc ggcatcggtg atgatgacca
     1681 ttttgccggc tttgatgtcg gctaggattt cggggatggg ggaaatatgg gacatggtgt
     1741 gttttcctgt gtgttcgggc gggcgggcgg tgtgttcggt atcgagccaa aaggcgggca
     1801 tttttgccgc ctgttcgatt ttacgggctt ttttctcgcc gaaggatttg tttttcaaaa
     1861 gggcggagag ttcgcctgtg ttgagggcaa cggcttcggc aaaacgggtt tgcaaaccgc
     1921 cgtagtgttc tgctatccat tgccgcaggt tgcggcggcg cagaccggct gtgtcggtca
     1981 tcgtttcatc ctttgtagaa atcggatgta gcggattgta agcgcgtatt taccaaaaag
     2041 caaatctgtt tttttgccaa accgaattat ttgctttttg gtaaacagat gccgtctgaa
     2101 gccggattcg tggcttcaga cggcattgcc gcaatccccg ccggcggggc ggctgtttct
     2161 tcccgcccgc ttccgattcg gggtttgccg gaagcttatt ccgaaaaaaa tttctgataa
     2221 acggccttga ttgccgggta cagcataggc ggtacgagca ggcgcaatat cggcaatcct
     2281 atgatgaatg cggtcttccc gataaaaacc ggatggattg ccttcgcgcc gaatatcttt
     2341 ctgcatttct ctttaaatga aaaatattct cggtagacaa cgataatgtt tttggaaaaa
     2401 taaagcctta ccgatataga tctcagatag ccgatattgt cgaagacaaa tttttgcaaa
     2461 ccgccgtaga tgggggaatt tttgttttcg actaaaaaat ccacgccccg gtcgagatgc
     2521 gtcagcatat cgctgaaatt tttgtatttg atgttgtggc tgatagagtt gccgagaacg
     2581 cggtactggt aaaaagggtt atcgtaaaag gcaaaagtct tgataaaacg cgccaattgc
     2641 aaactgtacg ggaaatcctc gtgaatgtat ccttttggga aaaacagatt gtttttgatg
     2701 attatttccc gcctgacaat ctttgtccac gcgttggcga tatagtaccg cccctccacc
     2761 agcgtttcaa aatggcggac aaaatcatta tccgaaaagt ccgccccttt ggggatgtcg
     2821 cggtaattga aggacgaggg atgcaggatc aaatcaacct ttttgtctgc aagttgttgt
     2881 aaatcaaaga gaatcccccc ccgcgcgttt tttgaacgat tggtgtcggc ccaatagtcg
     2941 tcgctgtcca aaaagattag gtaatcgcct tttgccgccc ggataccggc gttgcgggca
     3001 tccgacagcc cgccgttttc ttgatgaatc acttttatat gcggatattt gcctgcatat
     3061 tcgtcgcaaa tcttcccgca gccgtccggc gaaccgtcat cgaccaaaat catttcataa
     3121 tcggcaaaat tttcggcaag cacggaatcc acgcagcagc ggagatattt ttccacattg
     3181 taaataggga cgatgatgga gaaaatcata aatatcaatc cattatatta agatgtttgc
     3241 gcgtatgcct caaacccgca cccgcaatgc gtttgcatcc actataaaac ttattgcttt
     3301 atcaaggtat ggaaacctgt ttcccgaaag gcggcgcagg atgcccgttc cctgcaactt
     3361 tgccttattc cgacatcaga tgtcccagct tgtctgcttt ggtttggagg tagcgtttgt
     3421 tttcaaggtt ttccccgacg tgcaggggga tgcgttcgac cacgttaatc cccgcatctt
     3481 tcagggtttg gattttttcg gggttgttgg tcagcagttt gaccgagcgg atgcccagat
     3541 attcgtagat agattgcgcc aaacggaaat cgcgggcatc gacgggcagc ccgagtgcca
     3601 aattggcttc aaccgtatcc ataccttggt cttgcagatg ataggcgcgg attttgttaa
     3661 tcagcccgat gccgcgtcct tcctgacgca gatagacgat gatgccgcgc ccctctgtct
     3721 gtaccgccct catggccgct tcaagttgag gtccgcagtc gcattttctc gagaacagcg
     3781 catcgcccgt caggcattcg gagtggatgc gcgtcagaac cggattgccg tctgaaaaat
     3841 taccgacggt cagcgcgacg tgttcctgcc cgtttgcctc ttcaaagccg tgcatcgtaa
     3901 atacgcccca ttcggtcggc aggcggcagg atgccacatg gttcaacatt tcagacatct
     3961 tcactcccat cttttccggc agacataccc aacagcagtt tcaaaggttc ggacaaagcc
     4021 aaggcaagag ccacccattc cacctgtgct gccgtacccg cacactctgc gcattcaaat
     4081 tccacatgaa ccacgcccaa cacaccgcca ctttccgtgc agattggaat ggaaatttgc
     4141 gccgccgaag catgattgcg ttctcccgaa agctctccca aatccaacca acggcgtaca
     4201 tccgaggcaa cattcatcca gccgctttgc gcgcaacggc aggcaagtga aacatcggca
     4261 acattttcat ctagattcca tatgttttcc aaaccttctc cctgttggga caggcatatc
     4321 agttggaaag cctggttttc agacggcatc aaggcataga ccgccgcact ccgcacgcct
     4381 gtcgagcgcg aaaacaccga atccaaagcc atgaaaagcc gtttcagtgc atcttcgcgg
     4441 acattgtcgc acgacaggta atcggcaagt ttccaaccct catcactgcg ccacaaaacc
     4501 gaacggtcta tcgaagccgt ccccataccc attaccgtct gcgccgtcag atacgccgcc
     4561 cgaacctcgt caagcggcag cttcaaaccc tgagtcagca gaaaatcctt aatcaaagca
     4621 gcaggcatac ccaaattccg tcaataaaaa caaaaaaccg cccgattccg atgtcaggcg
     4681 gacgaaaaac agattttacc gccccaaacc ccaagccatc aacagccccg ccgaaaacct
     4741 tgcacaaaaa aacaaaatct gtataatcgt ccaattccca tgcggacgtg gcgaaattgg
     4801 tagacgcacc agatttaggt tctggcgccg agaggtgtga gagttcgagt ctctccgtcc
     4861 gcaccaataa tgataaataa attatatagt taatgcttat cgggacaaat tagtgccaca
     4921 aaacataagt cgtctgcttt tcagcgggcg gcttttttat ctgcctatat gtttcaacac
     4981 gcagaacggt acataaagtg ccgccatttg ttccgcaccg aatcggaaac gggtgcttcc
     5041 gcccgcaatc agattcaccg cccctaatgc cggggtttca accggaaaga agaaaatatt
     5101 gattttgatg atggcatcaa aaaacccctt ccgctcgtct gccaaaagga atggttaaga
     5161 aaagcaatgc gtcttttgat ggaacgcgat atataaggaa ttgaggggaa gggcaggcgg
     5221 gtttctgccc gttaatcctg cggacggtcc cgccaaaccc gacacgcgcg ggtatatggc
     5281 ggactgttta caatattgtc taaaaatctg aacctgcaaa cgtgaaataa ggtagaatac
     5341 gccccgttat tttaccgtcc aatccccatt taccaaggaa aaacgatgag tacttcattg
     5401 agttaccgcg atgcaggtgt cgatatcgac gcaggcgacc aactggtcga aaacatcaaa
     5461 ccgtttgcca aacgcaccat gcgtccggaa gtattggggg atttgggcgg ttttggcgca
     5521 ttggtcgaaa tcggcaagaa atataaaaat cccgtattgg tcagcggtac ggacggcgtg
     5581 ggtaccaagc tcaaacttgc cttcgattgg gataaacacg atacggtggg catcgacctt
     5641 gttgcaatga gtgtcaacga tattttggtt caaggagcag aacctctgtt tttcttggat
     5701 tatttcgcct gcggcaaatt ggatgttccg cgcgcaaccg atgtcatcaa aggcattgca
     5761 caaggttgcg aagaatccgg ctgcgccctg attggcgggg aaactgccga aatgccgggt
     5821 atgtatcccg tcggagaata cgatttggcg ggttttgccg tcggcgtagt ggaaaaagag
     5881 aatgtcatta ccggccgcag catcggcgcg ggcgatgtgg tattgggttt ggcttccaac
     5941 ggcgcacatt caaacggcta ttcccttatc cgtaaaatca tcgaacgcga caatcccgat
     6001 ttggatgccg agtttgataa tggcaaaacc ttgcgtgagg ctgttattgc gccgacccgt
     6061 ctgtatgtga aaccgattct tgccgctttg gaaaaattta ccattaaagg tatggcacac
     6121 attaccggcg gcggcattac cgaaaacgtg ccgcgcgtgt tgcctgaaaa cacggttgca
     6181 caaatcgatg ctaaatcgtg ggaattgccc aagctcttcc aatggcttca aaaggcgggc
     6241 aatgtggaaa cccaagaaat gtaccgaacc tttaactgcg gcatcggcat ggtcgttatt
     6301 gttgctgccg aagatgccga tgcggttcag ggtctcttgg gtgaacaagg tgaaacagtt
     6361 taccgtttag gtttgatccg tgagcgtcag ggagacgagc atcaaaccca ggttgcctga
     6421 ttgcttctat agcgaaatgc cgtctcaaac atgggtttgg acggcatttt t
//
LOCUS       DAXHRI010000113         6375 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXHRI010000113 DAXHRI010000000
VERSION     DAXHRI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.113"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..108)
                     /locus_tag="ACFM75_001590"
     CDS             complement(<1..108)
                     /locus_tag="ACFM75_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6256106.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGINKN"
     gene            175..462
                     /locus_tag="ACFM75_001591"
     CDS             175..462
                     /locus_tag="ACFM75_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256107.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLEEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(544..2631)
                     /locus_tag="ACFM75_001592"
     CDS             complement(544..2631)
                     /locus_tag="ACFM75_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC6256108.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAIFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2634..3899)
                     /locus_tag="ACFM75_001593"
     CDS             complement(2634..3899)
                     /locus_tag="ACFM75_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6256109.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(3906..4082)
                     /locus_tag="ACFM75_001594"
     CDS             complement(3906..4082)
                     /locus_tag="ACFM75_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256110.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGII
                     ILIIGVFLSIFFKK"
     gene            complement(5123..5359)
                     /locus_tag="ACFM75_001595"
     CDS             complement(5123..5359)
                     /locus_tag="ACFM75_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6256111.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGFGVQFSKPTFGFSKKW"
BASE COUNT         2032 a         1124 c         1102 g         2117 t
ORIGIN      
        1 atttttatta atgcctacta actctgctgc tgttcttgca gttacacctg cgacaaatag
       61 ctcaatgagt ttattttgtt tataccggct tagacgactt tttctcatag ggataattct
      121 aacttaattt gaatttccct agttatctag gacagcccct taaaaaatat ccctttgaaa
      181 gattttgatg aagtttgtga aaagtgggag aaatccttta aagaaaatgg aaaagaaatt
      241 gaatataatc aaaaatattc aagaatttat ggtgaagttc gtggagctgg ctttaatggc
      301 gatatcggag aaaagctaca tcgttgtgcc gtgatggaat gggctgaaaa tgagaccgtg
      361 aaattagaag agcagaaaaa acaacaaatt caacagaaaa aggaaactga aaaatcgcca
      421 aaacacaaag ccagtcgtga tgattgggaa atggaacgtt aaaccgctat tttcatgtat
      481 tctattaaat tatttgaaaa aagacttcac tttttgttaa agaaaaatga agtcttgttc
      541 tgttcaacct aaattcagaa ttttatctgc cgattgaatt gtttcttggc ggtgtgcaac
      601 cattattttc ataataccaa gactttttag gttatggtta attttttgtt cattttctac
      661 atctaaatgg ctacttgctt cgtctaaaaa tagaattttg ggtcgtttat acaatgcacg
      721 agccaagata actctctgct tttgtccacc tgataagata tttcccatat cgccaatcaa
      781 ggtctcatag cccattggca ttttaagtat atcgtcatgt atttgtgcca tttttgcaca
      841 ttgttcaatg agctccatat ttgggctttc atcaaaaaat gaaatatttt ccccaataga
      901 acctgcaaaa aggacatcat cttggcgaac aatcccgctc aatcccctaa taaaggatgg
      961 agaaacttga tatatatcat gcccattaat actaactgta ccagtttcag gttttaggct
     1021 acctgttaaa atgtttaaca aagtggactt cccccgacca gattgtcctg ttaaaacaac
     1081 tgcttcatta tctttaaatt ccaaattaat gttttcaaaa agatatggct cattatcagc
     1141 atatctgaat gagacgtttt caactttaag aaccagttgt tcattatcta atttaggtat
     1201 atgattatac ttaataattt cagtttctgt ttcatttaaa gtaatgtcag ccaaacgttc
     1261 agcatgaagc cctaacattt tgatttggat gtattggtca acgagagaag ctgttctgct
     1321 ttcaaattgc cctttataag ccaaaaaagc catcagaaca ccgactgtaa atgaaccatc
     1381 taaaattgcg cttgcaccaa gataaattat gataatattt tccatgctaa acaacagttt
     1441 atttgaaaat tcaaataaag cagagagttt atctgttgtc agcttggtat tgactgtatt
     1501 cacaaatagg ctcatccaag tgccatgtct ttgataatgt ttatcaaata atttaactga
     1561 ttggatacca cgaatggttt ccatgaaata tgagttttgt ttggcttcat gaacaatatt
     1621 ttcttctgtt gcatttctta atgggtaata tgcaagccaa cgaattagta tgtacaaaac
     1681 aagtgttaaa agaacaatca gcgataattg agtgctgtaa attgtcatta acacgaaagt
     1741 aaaaatagcc attaagctat ttaaaactaa aacaaaaaaa gtagaagtta gtgtttcttg
     1801 gatatgatct attgaaccaa atcttgaaat cacatctcct aaatgtcgtt tactgaaata
     1861 gtcattaggt aagtcaagta accttttaaa tatattggct ttccattgta aattaagagt
     1921 tgtagatagg tgcataccta cccatgcttg taacaggcta attaactgtt gcaggatagc
     1981 cagtaaacca aatcccaaag taagggtcag taataaattt ttatcagcag ttacaatgac
     2041 atggtctatt acccattgca taaagaatgg actaaccaat gcaaagactt ccaaagaaat
     2101 agctaatata agcatttgaa ttaaagagcg ttttaagcct gaaatccccc ttaatagaga
     2161 taatattttg attttctttg tttctttttt ctcttcaaaa tgggtattgg ggaataattc
     2221 tagggcaatc cctgtgaatt tttgtgaaac ttcgtccatt ttgatttttc gcataccgac
     2281 agcagggtcc ataatgacga tactgtcttt ggaaatggaa caaagtacaa caaaatggtt
     2341 taagttccaa tggagaatgc agggtagttg taaatttgac agctcatcta actctaaacg
     2401 caaagctcgt ggcgttaaat tcatttcatt gccaaatctc atgatgtctg caagatttgc
     2461 gccctttaat gacagggtgt atttttggcg taacgttctt aaatcagtgt gaaagccata
     2521 ataggacaag atggatgtca ggcatgctaa accacattca gcaacttctg tttgcagaat
     2581 gacaggtagc tttttgttaa atccaaagga cagtcttgat aaataatcca tttttaattg
     2641 atttttcctg aaatgctgta aagtgggtca agtacccatt cgtacaattt ttttcgttca
     2701 tggagaatat ctgcttctaa aatcatgcca atttgaagcg gcttgttttc tccgtatgct
     2761 ttaatcgttt gtttttccaa tttaactttc acaagatagg caggttcatt taataaggtt
     2821 gggttagtga aaatgatacc taaacctgat agctcttgtt taccgagagc agttctggca
     2881 actgaaataa tttctcctgt ggcatgtcca aatttttggt aagggtacgc ttggtaacgt
     2941 aaaacaactt tatctttcgg tttaataaaa ccaacagctt tactgggtat gtaaagattg
     3001 gcgaccaatt cagtttgttc agggacaatg cttaacagca atttagacgg ttcaacttgt
     3061 tgccctatat caacattaat tgttgaaata taacctgatt tactagctcg tatggtttgt
     3121 tcggatttca aatcaaaatc caaaatttct tggttcattt ccgtaatcgc acggttgagt
     3181 tggctcaatt cggttttatg gcgttcaggc aggctgctta atgtgatttt ctgttcatca
     3241 agttccctga ttgcattatt ttgttcacgt tttaggctct ccaaacgtga gcgttgttcc
     3301 aataaatggc tttcggcggt catcttatct tgttgggata ctgcgccttg actggctaaa
     3361 aacttgttct tgttaagggt tttttccgct aaacgaattt gacgattttg ccctgtaatt
     3421 tgctgtttaa tattctctaa ttgattgttt aaacgatgaa tgttgttatg aacatttttt
     3481 tgctcatttt gatgaatgcg ctttaaacgt tccaattctt gtaatgccaa agttttttta
     3541 aggttggctt ctgctgccaa tttggcttgt acgtttcctt tttcgccaaa acgcgatgtg
     3601 gaaagtttga acaatggttc gccagctttg acaaagttac catcttcaac aaatttatgc
     3661 gtaatcgtgc cgatatcgga agagtaaaca cgaaccaccc ccatagttgg aagtaattga
     3721 ccttcaacgg ttgttttatt ggtatagcta ccaaaaatca aaaagataat gatacacaga
     3781 gcaatgagaa aagcgcaaaa agtcagaaat aaaaaagaga atggacgggt caagataacc
     3841 tgacctgtcc acttattttg ttgggctaca aagacttctt ttcggaataa gtgggacatt
     3901 tattattatt tcttaaaaaa aatggataga aaaacaccaa taattaaaat aattatgcct
     3961 aataacataa atttgctatt gattgatgtt tctattttaa ttacattaaa tttaaccaat
     4021 aatccagaaa tggttgcagc taaaatgcaa aagacagtgg cgtaaatggt ttcagtattc
     4081 ataatttttc tctttcaaaa ttgttcaatc taaatagaaa aaggtaactt caatacatat
     4141 tgctgtaact gaaattacct tttccttaat gatgtaatta tcttatggct tcatcatgcc
     4201 acgaccgata ttgtatccgc cagcagcaga gaaaatacca gctccaattg cgccaaccca
     4261 gcctactggt cctgtccact tgttccgttg agctacaaag acttctggtc tgaaaaatga
     4321 gtgattgtta gacatgtgta cttatttatg tttctttgat tgggataaat ttgtaaaaac
     4381 aaaaaaaagg caagttgtta ttatagaaga acttaaaatg cttaacatac tcattgaggt
     4441 ttgtaccaca taatgagata cgagataatt tgttgggagt aaaatcaaaa aaaagattaa
     4501 atatttaaaa taatcactat attttttcat tatttgttcc tttttgtatt taagttctta
     4561 tgaaaaacta gggtattttg aatagaaaat atcaaaatac cctagtttta ttaaggaata
     4621 ttaccaccaa caagtgcttc catattgtac agcaccagtg attgcaccac caatagctcc
     4681 gaatttcgca cctggaatag caccaatacc acctgcaaat gaaccaacaa ttgccccacc
     4741 agctgctcca cctaatgcac taccaatggt attttttgag aaatcacgcc agttacaagc
     4801 agcaccagaa acttgttaaa tttcattcaa agtcaaaact tgcataatat tcacctttgt
     4861 taagtgtggt tgagaaaaaa gtaaattttc atcgttactc attatttgca atcatgcata
     4921 tgctgtaacc aagcactata ttcagttgga tcatagtttt tactactggg ttgtctgata
     4981 tgactattgg cactattggc acaactggga ttagcaactt tattgtgagt acctaatgga
     5041 ttgatatttc ctgtaaagtg gctattgcat gcgcttaatg agcatagatt tattaatgtc
     5101 agaaaggata aaacaaaaaa tcttaccatt ttttactaaa accaaaagta ggtttcgaaa
     5161 actgtacacc aaaaccttgt acatatccaa attgcttgct aacagaaaac tctactccac
     5221 tattgttagg ggaataggaa gcaccaattt taccaacagc aaccatatct tttttaccta
     5281 ggttgccgcc accctgtgca gagatatgga atttgccacc tgacacttca actaattcag
     5341 aggtgtgtaa ctctttcatg attttctcct gtaaagaagt atggttggaa atgtaaatca
     5401 gaattaacct ttctcaagtt taatttaata cttttttttg caagtatttt attttaaatt
     5461 aaatcaatat tttaaaaatg tttacttgta tactgaaaaa gaaactccta atggtggagt
     5521 ggggcttttg tttaagtaag cattgaaaaa atttaaattt cccattatga ttaacttaat
     5581 gcaagagctt tttcaggacg tacaaaccag cgaacgggcg ttctgatgtg ggcaggttgg
     5641 ttgtcgctaa ccaatttcag tagctcaatc gtcagattgg tatccgaccc tatcaagcta
     5701 aggagaggct gcggctacta cacaaagcac ctgaaattat tgggtgcttt tgtcttttta
     5761 tggcattgta ccaatttgtg taccacgagg gcttgttcgc ccaaacgcgg taacaactga
     5821 atacacactt tccccctcaa cttctttgcg ttctaaaacg actgtcccaa tccgagaata
     5881 gacaatcata ggacgatgat tgccccttac atcactaaat tcactaaaaa tttttgcgcc
     5941 tgattgaaaa atcatttcca cataaaaggg ttacacctgt ttcatcgtgg cttaatccaa
     6001 accgcgttaa atctgctttg tgttctgcca aaatgtgaat cacaccaaag ccgtgattaa
     6061 cctttttgtg ctcgccgtgt tttaaaacaa tttgacctgc cgggaagtct gctctattgt
     6121 tcggacaata gggggaatat accaattact ttaactacat cattttttga catttttctt
     6181 gcctttcaat ttaaaatcca aaatcatact gccataattt agcaatccaa aaaaatttag
     6241 gcagcagtca tggtttcgta ttttagaaaa cgaaacttgg tttggtgttt tgaagcattt
     6301 cagtacacaa acatggaatt ttaaattgtc ttgtgttgcc ttgatttagg atgttccagt
     6361 tttttaataa atagt
//
LOCUS       DAXHRI010000114         6313 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXHRI010000114 DAXHRI010000000
VERSION     DAXHRI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.114"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(55..1923)
                     /locus_tag="ACFM75_001596"
     CDS             complement(55..1923)
                     /locus_tag="ACFM75_001596"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6256112.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTASSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGCYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(2003..3373)
                     /gene="purB"
                     /locus_tag="ACFM75_001597"
     CDS             complement(2003..3373)
                     /gene="purB"
                     /locus_tag="ACFM75_001597"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC6256113.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMSNAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIESLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(3445..4077)
                     /locus_tag="ACFM75_001598"
     CDS             complement(3445..4077)
                     /locus_tag="ACFM75_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC6256114.1"
                     /translation="MGNTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFAFRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
     gene            4233..6113
                     /locus_tag="ACFM75_001599"
     CDS             4233..6113
                     /locus_tag="ACFM75_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFC6256115.1"
                     /translation="MNIFYEESGQFKVAAIVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFSAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGMKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPSISIYFDVDGE
                     FNVGAPTCKIEAVNIAENLRIQTIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT         1591 a         1716 c         1644 g         1362 t
ORIGIN      
        1 ccgccgcccc gaaaggcaaa cggcggcaca aacgatatcg gacggcaagg caggctactg
       61 caatgcgccg tcgcgagaag atttaagcag gcgttcgtgt ttgtgaaatt cccgccccat
      121 ataataagaa ccgaaatagg tcaggtggtc ttggtcgccg taaagatagc agccgtgtat
      181 ttcgaccgtg tttttaggca ggtatttctg tgcgtccacc caatgcacat tggggatatc
      241 tttaaccaaa tcaaagaccg cctgattgct cttgccgatg tcgcccatag cctgaatggg
      301 gcggagatat tggtttgcgg caaatctttt caatttttcc tccctcaggg gcgaacggct
      361 gattgatgtg ttgtttgcaa aaacatagac gggtttgacg gcggctatcc ttttgacggt
      421 ttccctgaat cgggcttgga acccgggtat taggaaggat tgcgcttcaa atctcggcac
      481 gggctggccg cccatcctca aatcatagaa ttgggcaatg aaaacggctt cggctttttc
      541 aacttcatcc cggtattttc gacataacgg gttgtctgcc agcttctcat ctacccaaac
      601 caaacactcc gaatcgaggg acaggatttt ggctttccac ccttcccggc tgccgacata
      661 atccagaaac ccccgcaggt gtccggcgtg cgagtcgccg agggtcagga cggtttccgg
      721 aaaatgattt tccgcagcaa ggggcgcgcc gggcaacggg cggaggtgtt cctgtttcaa
      781 tatccccctt gcgtacaggt tgtaaccgac aagtatcagg gacggggcga gatagaggca
      841 gaaaaatgcc tttttgaagg tcatcttccg ttttctaagc ggctgttcaa tcaaataata
      901 actcaacagg gaaaatccgg ccgtcaacgc ggcaaccgcc gatacggcag gcagtccgag
      961 ctgtttgtcg cctgtaatgt aatgggcgaa agcaataaaa atccaatggt acaggtatag
     1021 ggaataagag attttgccga caaatacgat ggggcttgcc gacaggatgc gggtcggaag
     1081 tgtcccgtat tgcatactcc ggataagcag tgccgtcagc aggcagggaa ggagcagggt
     1141 cattcccggg ataaacggat tgtgtttgtc aatcacgaac aggcaggcaa gcaatgcgcc
     1201 gaagcagagt gatgaaagca actgccgttt tccatttgct gtttgccgtc tgccgttttg
     1261 cgtttgcccg taaaccgcca gcagcgaacc tgccaacagc tcgggaaacc tcagtgtcga
     1321 aaggtaataa gtattgggtt ggttgaggat gtcggtataa aacccgcttg gcaaaaacga
     1381 tgaggcagtc aaaatcaaaa acaggatgat gctgatgtta cgcagcaccc gtagcgattt
     1441 ggtttttttg cagcaaaata tcagcaaaag gggatacagg aggtaatact gttcctctac
     1501 cgccaaagac cagatatgca gtacggggtt ctcgtcggca ctcaaatcga aatacccctg
     1561 ctgaaacccc agataaatat tggacaagaa aaccgcagaa agctccacgg ttttccgcat
     1621 ttggttgaaa tcttcgtaaa ggaagatttg agaggcaatc accgaagcca gcgacacggc
     1681 cgcaataaag gcagggtaaa tccgcttaat cctgcgggta taaaaatccc ggaaagaaaa
     1741 agaaccgttc tgtatttcag aaagaatgat gccggtaatg aggaatcccg agatgacaaa
     1801 gaaaatgtcc acccccaaaa accctccggg cagccagcgg ttattcaggt ggaaaataat
     1861 gacggatagc acggcaacgg cccgcaatcc gtcaatttca ggcctgtatc ggacagcttg
     1921 cataaatatc gccccgtatg tcccgttatc ttaaaaatgc cgtctgaacg cgcgttcaga
     1981 cggcatcggt ttcagtaaac gctcaaatcc gtttcgccaa cgcttcagcc ttgcccacat
     2041 acagtgcagg agtcagctca agcaattttg ctttggcttc taccggaatt tccaacgatt
     2101 cgataaagcc tttcagcact tcgggcgtga tgccgccttt gccgcgcgtc aggtctttca
     2161 gtttttcgta aggattggcg acaccgtaac ggcgcattac ggtttgaatc ggctcggcga
     2221 gcagctccca agtggcatcc aaatcggcgg caagcgcggc ggggttgggt tcgagcttgt
     2281 tcagaccgcg caggtgggcg gcgaaaccca atacggcata gcccacgcct acgcccatat
     2341 tgcgcaatac ggtgctgtcg gtcaggtcgc gctgccagcg ggaaatcggc agtttttcgg
     2401 acaaaaagcc caatacggcg ttagacatac cgaggtttcc ttcggagttt tcaaagtcga
     2461 tggggttgac tttgtgcggc atggtggaag aaccgacttc gcccgctttg actttttgtt
     2521 tgaagtaacc caatgaaata taaccccaaa cgtcgcggtt aaagtcgatg agaatcgtgt
     2581 tgatgcggct gagggtttgg aagaattccg ccatatagtc gtgcggttcg atttggatgg
     2641 tgtaggggtt gaaggtcaga ccgaggctga tttcgacaaa gttgcggcag tgggtttccc
     2701 aatccacatc gggataagca accatatggg cgttgtagtt gccgaccgcg ccgttgattt
     2761 tgccgaggaa ttcttgcgct tgcaggtttt taaactggcg ttgcaggcgg tacacgacat
     2821 tggcggtttc tttgcccaaa gtggtcggcg tggcgggctg gccgtgggtg cggctcatca
     2881 tcgggacggc ggcaaggtcg tgcgccatag cggtgagttt gccgatgatt tctgccagtt
     2941 tgggcagcaa aacagcctca cgcgcttctt gcagcattaa agcgtgggac aggttgttga
     3001 tgtcttcgct ggtgcaggcg aagtggatga actcactcac ggcggcgact tcgggcactt
     3061 cggcaaaacg ttttttcagc caatattcga tggctttgac atcgtgattg gtggtggctt
     3121 cgatggcttt gacggcggcc gcgtcttcca atgaaaagtt ttcaatcacc gtgtcgattt
     3181 cggcaagcgt ttcggcactg aagggcggca cttcggcaat cttcggctcg gcggcgaggg
     3241 ctttgagcca gtttaattcg actttgacgc gcgccttcat caggccgtat tcggaaaaaa
     3301 tcgggcgcaa tgcttcaacg gattgggcat aacggccgtc taaaggggaa agcgaggcga
     3361 tggggttgat catttcggca tcctgttcgg gaaagacatc aaaaattgtt aaattgtttg
     3421 caattataca tcactctcag gacgctatgc cgtctgaagc ccgtgttccg acagatacgc
     3481 aacgatttcg tcttcatcgt ccgatatggc gaacaaagag gctgcccctt cggaaatcag
     3541 accgcgcgcc aaaagctgcg cgtttatcca ctccgccaag cccgaccaaa acgcctttcc
     3601 gaccaaaaca atcggacgcg gcggcacttt gcccgtctgt accaaggtca ggatttcaaa
     3661 caattcgtcc agcgtcccga agccgcccgg catcacgaca tatgcttggg aatagcggaa
     3721 aaacaccgcc ttgcgttcgg caaaacggga aaaccgcaag gcgatgtcct gatacggatt
     3781 cggtttctgc tcgtgcggca aaacgatgtt cagccccacc gaaaccgact tccctgcaaa
     3841 cgcgcccttg tttgccgcct ccataatccc cggcccgccg cccgaaatga cggcaatgcc
     3901 cgaatccgac agccgccgcg ccagacggaa ggcgaacgca taatccgcat gattctgcgg
     3961 cgtgcgcgcg ctgccgaaga tactgactgc cggaaacacg cccgccaatg cttcgtctgc
     4021 ctgcctgcgt tcggcatcat aacgtgcctg ctccggcaca cggtttgtat tccccattcc
     4081 gtcctccgtt caaaaatggc gattgtaccc cgtcaaaaac gtaacgcaag caccgcaaag
     4141 acgcgccaac cctcccccaa ccttttttca gacggcattt tcggtaatct gctaaaatcg
     4201 cccgcttgag tttccacaga aaaatccgaa aaatgaatat tttttacgaa gagtccggcc
     4261 aattcaaagt cgccgccatc gtccaaaaaa acgacgccac ctaccaagtc gatacccaac
     4321 acggtaaacg caccaaagtg aaggcaaaca atgtctttgc cgagtttgac ggcgatatgg
     4381 cggcgttttt ggaaaacgcg caggcacagg cggcggacat cgacaccgat ttattgtggg
     4441 aagtatgcgg cgaagaagaa ttttccgccg aggcgattgc cgaagaatat tacggccatg
     4501 cgccgaccaa aaccgagctg gcggcgacct tgattgcgct gtacgccgcg ccgatgtatt
     4561 tctacaaaaa agccaaaggc gtgttcaaag ccgcgcccga agaaacttta aaacaagcac
     4621 ttgccgccat cgaacgcaaa aaacagcaag acgcgcaaat cgacgcttgg gcggaagcct
     4681 tgaaacgcgg cgagatgccg tctgaaatcg cggcggattt gaaaaccatc ctgcacgcgc
     4741 ccgacaagca gtcgctgacc tacaaagcct ttaccaaagc cgccgacgcg ctgaaaacct
     4801 ctgcctacga attggcgaaa aaaacgggcg gcattacgtc cattccccaa tacctgcaag
     4861 acgggtttga aatcaaatat ttccctaaag gaacaggctt ccccgacctt ccgcttcctg
     4921 aaatgcccga cctgcccaaa gctgacgtta ccgccttttc cattgacgac gagtcgacca
     4981 ccgaagtcga tgatgccttg agcctgaccg acttgggcaa tggcatgaaa cgcgtcggta
     5041 tccacatcgc cgcgccgtcg cttgccatta agcaaggcga caaaatggaa aaaaacatca
     5101 tggaacgctt aagcacggtt tatttccccg gcggcaaaat caccatgttg cccgaaaact
     5161 ggattgccgc gttcagcctt gatgcaggcg cataccgccc ttccatcagt atttatttcg
     5221 atgtggacgg agagttcaac gtcggcgcgc caacctgcaa aatcgaagcg gtcaacatcg
     5281 ctgaaaacct gcgcattcaa accatcgaac cgcatttcaa cgccgaagcg ggcttggacg
     5341 aagcgggcga aatgatgttc gcccaccatc aagacctgat ttggttttat caattcgcca
     5401 tcaccctgca aaaagcgcgc ggcaaatacg aacccgaccg cgcgccgcaa tacgattaca
     5461 gcatcgaatt ggatgaggaa ggcaaagtct ccgtcgtccg ccgcgaacgc ggttcgccca
     5521 tcgatacgct ggtcagcgag atgatgattc ttgccaacag cacttgggca caaatgctcc
     5581 atgacaacga cctgcccggc ctcttccgcg tccaacccac cggaaaagta cgcatgagca
     5641 ccaaatccga gccgcatatc ggcatgggcg tgcggcatta cggctggttt acctcgccgc
     5701 tgcgccgcgc cgccgactac atcaaccaaa agcagctgat cagcctgata gacgacactg
     5761 ccgagccgct gtatcaaaac agcgatgccg agcttttcgc cgcactgcgc gactttgatg
     5821 ccgcctatac cgcctacgcc gacttccaac gccaaatgga agcctactgg agcctggttt
     5881 acctgcaaca gcaaggcacg agcgagctga cggcgacgat tctgaaagaa gacctcgtcc
     5941 gcatcgaagg tctgccgcta gtaacacgcg cgaccggtat tccgtttgac gcgctgccca
     6001 aatcacaggc attgttcaaa atcaccgaat tggatgccga gaagcagttt gtctcgctca
     6061 actacatcaa ggcagtcgca cccgcgggta caacggcagg caatgccgtc tgaagcccga
     6121 tacggcaaac tccggcaaaa acagaaacct gaatctcatc attcccacag tcgggaatcc
     6181 ggttttttgg gtttccgttg tttttccgtt tcaatgaact tccaagccgc cgttcccgat
     6241 aaatacccgc aatctaaaat cccgtcattc ccacgcagat gggaatctag gtttatccgc
     6301 acagaaactg ata
//
LOCUS       DAXHRI010000115         6261 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXHRI010000115 DAXHRI010000000
VERSION     DAXHRI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.115"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(391..825)
                     /locus_tag="ACFM75_001600"
     CDS             complement(391..825)
                     /locus_tag="ACFM75_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC6256116.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="ACFM75_001601"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="ACFM75_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC6256117.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="ACFM75_001602"
     CDS             complement(1556..2398)
                     /locus_tag="ACFM75_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC6256118.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="ACFM75_001603"
     CDS             2653..3291
                     /locus_tag="ACFM75_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC6256119.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="ACFM75_001604"
     CDS             complement(3743..4543)
                     /locus_tag="ACFM75_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC6256120.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4679..5185)
                     /locus_tag="ACFM75_001605"
     CDS             complement(4679..5185)
                     /locus_tag="ACFM75_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256121.1"
                     /translation="MDFFDFFAQGKYKKIATEQGLDRKYFIFHYIVCNLSAISSFCLM
                     KVIHSYTILCLLIMIIGIAFGFILRNRVLSIVKNKQKFLSDMFPLEMPFFSRVYKIPI
                     LARFLNRGMKWLYLQNFFCLVMICFSISLILNEERGWMPYFICYWFGLCYPANTYYVF
                     LSRDYKHI"
     gene            complement(5522..6163)
                     /locus_tag="ACFM75_001606"
     CDS             complement(5522..6163)
                     /locus_tag="ACFM75_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC6256122.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMMMSEKSGVPLYFDRAL"
BASE COUNT         1674 a         1568 c         1294 g         1725 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aatactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgagaaaa accactgctt ttcacgattt
     1681 taacggactc gacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 cgtcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt ggtaccggtt cccttatctt
     1921 ccccattacc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgcg gcaggcgcgc
     2221 ctgcaccttc gggtgcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttca cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctactg ctgatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccaa aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt gaccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gtttgctgga taacaaagtc
     4741 caaaccaata gcagataaaa taaggcatcc accccctttc ttcattaagg ataagagata
     4801 tcgagaaaca aatcataact aaacagaaaa aattttggag ataaagccat ttcattcccc
     4861 tattcaagaa tctggccaag ataggtattt tgtatactct agaaaagaaa ggcatttcca
     4921 agggaaacat atcagataaa aacttttgtt tattttttac tatagataga accctatttc
     4981 tcaagataaa tccaaatgca atgccaatta tcattattaa taaacaaagg atagtataac
     5041 tatggataac cttcattaaa caaaaagaag aaatggcaga taaattacat acgatatagt
     5101 ggaatataaa atatttacgg tctaaacctt gttcagttgc aattttttta tacttacctt
     5161 gcgcaaaaaa atcaaagaaa tccattaaac tatctttcac attagaaatt taaaagctaa
     5221 ataatacaac aaacaatgtg aagtactatt catggtttat ttaaaaaata atactattct
     5281 gaacattatt tagatacaga aattaacaaa ttagaactaa acaagctttt aaatacttta
     5341 attttattgg aaagctataa aaggaactat aactttacac actagtcact tctttttaag
     5401 aggcaaaagg gattgggaag gtcgtcttgg agataagcac tggtatttcg gccaatggta
     5461 aatagagttt acctcaaata gggtagaacc tccttcatct gtcagttaat aacagccact
     5521 tttacaatgc cctgtcaaaa taaagcggca cgcccgattt ttcactcatc atcatcaaat
     5581 aacccatcac cttttgaggc cattcgatac cgcgcaccac ggtcagattc ctcaaaacgg
     5641 ggaaaaccaa aatatcctcc atgccgattc cgccgttgat gccgtctgaa gcaccgtcca
     5701 tcaaattctc caactcttgc aaatctgcgt ttatgcgttc gagatattgg gcggttttat
     5761 tcaaattggc ggaaaagctg ccgatgcttt tctctttttt gtctgtaaaa tattttaccg
     5821 cttccggcgt tgcaaattcg ggcagcccga ttttgatcac gcgcggctgc accagtttgt
     5881 cgttgtatcc gcccaccttg tccagccacg cccgtatctc ggggcggact tcgtctttca
     5941 gacggtcttc gcggtcgaaa tgccgcacaa tgtccaaact ctcgcccata aacgaaccgt
     6001 cttctttttg caggacgggc acttgtttcg caccgatcat accgatcggc gttgcctcgt
     6061 cgtcgtttgc cagcacggct tcttcaacgt ccgcgccaaa cagcccggca gccatccgcg
     6121 cacgcacaca aaacgggcaa tggtcgtaaa tatacagttt catcaaaata ttcctcgtca
     6181 acctgtcggt accggctacc ttaacacccc gcgccacccg aaacaagttt atcttcccgc
     6241 ctatgcaccg taaataaata a
//
LOCUS       DAXHRI010000116         6258 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXHRI010000116 DAXHRI010000000
VERSION     DAXHRI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.116"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            35..1390
                     /locus_tag="ACFM75_001607"
     CDS             35..1390
                     /locus_tag="ACFM75_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC6256123.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVLAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="ACFM75_001608"
     CDS             1394..2404
                     /locus_tag="ACFM75_001608"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC6256124.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
     gene            2638..4896
                     /locus_tag="ACFM75_001609"
     CDS             2638..4896
                     /locus_tag="ACFM75_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC6256125.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALSGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPYDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLELQVYGKDI
                     TLNGSHTVALEK"
     gene            4909..5574
                     /gene="pgmB"
                     /locus_tag="ACFM75_001610"
     CDS             4909..5574
                     /gene="pgmB"
                     /locus_tag="ACFM75_001610"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC6256126.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LRANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRHCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSETAGLTYAYLQNVWAQ
                     SGR"
     gene            complement(5784..>6258)
                     /locus_tag="ACFM75_001611"
     CDS             complement(5784..>6258)
                     /locus_tag="ACFM75_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6256127.1"
                     /translation="ARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLPP
                     LLEGIAEGTTVYADKGYDSAENRQHLEEHQLQDGIMRKACRNRPLTETQTKRNRYLSK
                     TRYVVEQSFGTLHRKFRYARAAYFGLLKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT         1375 a         1799 c         1714 g         1370 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcttgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta ccaagaagcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ctagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg gcctttttat agtggattaa caaaaaccgg
     2461 tacggcgttg cctcggctta gctcaaagag aacgattctc taaggtgctg aagcaccaag
     2521 tgaatcggtt ccgtactatt tgtactgttt gcggcttcgt cgccttgtcc tgatttttgt
     2581 taatccacta taagatttca ccattccctc aaatcaatcc aaacaggagc ttcataaatg
     2641 tacacaagaa ttatggaaat cagcccttgg acgctgcgtt cggcaaagct ggaaaaagaa
     2701 cacaaacggc tgcaagagag cctgaccagt ttgggcaacg gctatatggg tatgcgcggc
     2761 agctttgagg aaacctattc cgccgacagc cacttgggta cctacatcgc cggtgtgtgg
     2821 tttcccgaca aaacccgcgt cggctggtgg aaaaacggct accccaaata tttcggtaaa
     2881 gccatcaacg cgctcaattt cagcaaagtc aaaatctttg tcgacggaca agaggtggac
     2941 ttggcgaaaa acgatgttgc cggcttctcc gtcgaactcg atatgcagca cggcgtgttg
     3001 cgccgctcgt tcaccgtatt cggtgtgcgt ttcaatgtgt gcaaattcct gtccgtcgcg
     3061 caaaaagagc tggcggttat ccgctgggaa gccgtatccg ttgacggcaa aacccaccaa
     3121 gtccgcatcg attccatcat cgatgccgac gtgaaaaacg aagactccaa ctacgaagaa
     3181 aaattctggc aggtattgga caaaggcgtt tcagacagtc tctcctacat tgccgcccaa
     3241 accgttgcca accctttcgg cgtggaacaa tttatcgtca acgccgagca aactttcgcc
     3301 ggcagcttca aagccctcag cggcagccaa accgactggc aagtctccaa ttctttcgag
     3361 gtcgaagtcg gcggcacgcc caaaaccttt gaaaaacggg ttattgttac caccagccgc
     3421 gattatcagg gcttggaagc agtgaaagcc gcaggccgcg ccttgtcgga aaaagtcgca
     3481 ggcgttgcgt ttgaaacctt gctggacgcg cacaaagcag gctggctgca ccgttgggaa
     3541 atcgccgacg tggtcatcga aggcaacgac gaagcgcagc aaggcatccg tttcaatctg
     3601 ttccaactgt tctccaccta ctacggcgaa gacgcgcgtc tgaacatcgg cccgaaaggc
     3661 tttaccggag aaaaatacgg cggcgcgacc tattgggaca ccgaagccta cgccgtgccg
     3721 ctctacctcg cactggcgga acccgaagtt acccgcaacc tgctgcaata ccgccgcaac
     3781 caactgccac aggcgcaaca caacgcgcgc gaacagggct tggcgggcgc actctatccg
     3841 atggtaacgt ttacgggcat cgagtgccac aacgaatggg aaatcacctt cgaggaaatc
     3901 caccgcaacg gcgcgattcc ttacgccatc tacaactaca ccaactacac cggcgacgag
     3961 ggctatcttg ccaaagaagg cttggaagtc ttggtcgaag tgtcccgctt ctgggcggac
     4021 cgcgtccact tctccaaacg cagcggcaaa tacatgattc acggcgtaac cggcccgaac
     4081 gaatacgaaa acaacatcaa caacaactgg tacaccaaca ccctcgccgc atgggtattg
     4141 gactacaccc gcgaagccct ggcgaaatac ccgcgtccgg atttgaacgt gagcgccgcc
     4201 gagttggaaa aatgggcgga catcagcgcg aatatgtacc gtccgtatga cgaagaactc
     4261 ggcgtattcg tacaacacga cggattcctc gacaaagaca tccgccccgt gtccgcgctt
     4321 tcgcccgacg atttgccgct caaccagaaa tggtcgtggg acaaaatcct gcgttcgccc
     4381 ttcatcaaac aggcggacgt cttgcaaggc atctacttct tcggcgaccg tttcaatatc
     4441 gacgaaaaac gccgcaactt cgacttctac gaaccgatga ccgtgcacga aagctcgttg
     4501 tcaccttgta tccactctat ccttgccgcc gaactgggca aagaagaaaa agccgtggaa
     4561 atgtaccggc gcaccgcccg cctggacttg gacaactaca acaacgacac cgaagacggc
     4621 ctgcacatta cctccatgac cggttcgtgg ctcgccatcg tccaaggttt cgcccaaatg
     4681 aaaacctggg gcggcaaact cagcttcgct ccgtttctgc cgagcgcgtg gacaggctac
     4741 gccttccaca tcaactaccg aggccgtctg attaaagtcg ccgtcggcaa agaaaacgtc
     4801 gtcttcaccc tgctcaaagg cgagccgctc gagttgcagg tgtacggcaa agacattacg
     4861 ctcaacggca gccacactgt cgcgttggaa aaataaggag ggcacaaaat gacgtttacc
     4921 gctgtgctct tcgatctcga cggtgtcatc accgataccg ccgaatacca ttaccgcgcg
     4981 tggaaaaaac tcgccgaaga actgggcatc ggcatcgacc gcaagtttaa cgagcagctc
     5041 aaaggcgtgt cgcgcgacga ttcgctcaaa cgcatcctcg cgcacggcgg caaaaccgtc
     5101 ggcgaagccg agttcgccga actgacccgc cgcaaaaacg acaactacgt cgagatgatt
     5161 caggcggtca aaccagaaga cgtgtatccc ggcattttgc cgctgttgga agcattgagg
     5221 gcaaacggca aaaaaatcgc cctcgcgtcc gccagcaaaa acggcccgtt cctgctggaa
     5281 cgcatgggac tgacccactt cttcgacacc gtcgccgacc ctgccgccgt cgcgcattcc
     5341 aaacccgccc ccgacatctt cctcgccgcc gccgagggcg tgggcgcgga catccgccac
     5401 tgcatcggca ttgaagacgc cgccgccggc gttgccgcca tcaaagccgc cggcgcactg
     5461 cccatcggcg taggcaaagc cgaagacttg ggcagcgaca tcgcgctggt gtccgagacc
     5521 gccgggctga cctacgccta cctgcaaaac gtgtgggcgc agtcgggcag gtaaagcgcg
     5581 tcagataaag tgtcaaggaa gtaaaaggcc gtctgaacag tgtttcagac ggcctgagac
     5641 ctttgcaaaa ttcctttccc tcccgacagc cgaaacccaa acacaggttt tcggctgttt
     5701 tcacctcaaa tcactcctaa ttctacccaa ataccccctt aatcctcccc ggatacccga
     5761 taatcaggca tccgggctgc cttttaggcg gcagcgggcg cacttagcct gttggcggct
     5821 ttcaacaggt tcaaacacat cgccttcagg tggctttgcg cactcacttt gagcagcccg
     5881 aaataggctg cccgcgcata gcggaattta cggtgcagcg taccgaagct ttgttcgacc
     5941 acataacggg tcttcgacaa ataccggttg cgtttggttt gcgtttccgt cagcggacgg
     6001 ttgcggcagg ctttgcgcat aatgccgtcc tgcaactgat gttcttccag atgttgccgg
     6061 ttttccgcac tgtcatagcc tttgtcggca tagacggtcg taccttcagc tatcccttct
     6121 aacaacggcg gcaggtgttt gcactcatgg gcattggcgg gggtgatgtg cagtttctcg
     6181 atatagcctt ccgcatcggt acgggtatgt tgtttgtaac cgagtttgta gaggccgttt
     6241 ttcttgatcc aacgggca
//
LOCUS       DAXHRI010000117         6181 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXHRI010000117 DAXHRI010000000
VERSION     DAXHRI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.117"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..335)
                     /locus_tag="ACFM75_001612"
     CDS             complement(<1..335)
                     /locus_tag="ACFM75_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256128.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTRADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNRNEHWTAIGGGALIG
                     AMNPINGGRA"
     gene            complement(378..1247)
                     /locus_tag="ACFM75_001613"
     CDS             complement(378..1247)
                     /locus_tag="ACFM75_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC6256129.1"
                     /translation="MKRIFLSALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRAALAAPVYIQTGATSFIPIPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKLGVQHTF"
     gene            1412..2335
                     /locus_tag="ACFM75_001614"
     CDS             1412..2335
                     /locus_tag="ACFM75_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6256130.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGATLDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSRFCL"
     gene            complement(2413..3054)
                     /locus_tag="ACFM75_001615"
     CDS             complement(2413..3054)
                     /locus_tag="ACFM75_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6256131.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVLARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLMGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(3109..3900)
                     /locus_tag="ACFM75_001616"
     CDS             complement(3109..3900)
                     /locus_tag="ACFM75_001616"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC6256132.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDNYLPDTRQTAVDINPQ
                     VIAIARNLFELPFESEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFYL"
     gene            4066..4141
                     /locus_tag="ACFM75_001617"
     tRNA            4066..4141
                     /locus_tag="ACFM75_001617"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4099..4101,aa:Thr,seq:tgt)
     gene            4176..4251
                     /locus_tag="ACFM75_001618"
     tRNA            4176..4251
                     /locus_tag="ACFM75_001618"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4209..4211,aa:Thr,seq:tgt)
     gene            4393..5184
                     /gene="panB"
                     /locus_tag="ACFM75_001619"
     CDS             4393..5184
                     /gene="panB"
                     /locus_tag="ACFM75_001619"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC6256133.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGILVCAHIGLTPQSVFAFGGYKVQGRGSKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMHGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            5174..5350
                     /locus_tag="ACFM75_001620"
     CDS             5174..5350
                     /locus_tag="ACFM75_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256134.1"
                     /translation="MQTEQGLLLMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            5307..6143
                     /gene="panC"
                     /locus_tag="ACFM75_001621"
     CDS             5307..6143
                     /gene="panC"
                     /locus_tag="ACFM75_001621"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC6256135.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAACLGTTRLIDNLEIK
                     LP"
BASE COUNT         1351 a         1382 c         1840 g         1608 t
ORIGIN      
        1 gctcttcctc cgttaatagg gttcatcgca ccaattaacg caccacctcc aattgctgtc
       61 caatgttcat ttcgattata cgagccacta gcaatagcac tagccatata gctgaaatgc
      121 cctccaactc ctcccataat gcctccaaca gcattaggaa tccactcccc ctcagtctcc
      181 ttcatctcct tttgagaaag ctccgccaac tgcatcggtg catctgcccg cgtgtggaac
      241 acttggtttt caaatgtctg attgtccaag ccgtttgcca ttgcgggggc aatcatagac
      301 agcatcatta cggctgcggt gatttgtttt ttcataacaa ctcctttgga ttacaacgtt
      361 ggaaaatcaa aacctgctta aaatgtatgc tgtacgccca atttcagttc ggaactgctt
      421 tgccctgaaa cgttgaaacg tgcggatgcg tttaaagccg tggttttggt gaaaccgaaa
      481 cctgcgccga aatgggcgta ggtggatgtg tttcttgcgg attctttttt gccgtctatg
      541 cggtcgggct gtttgcccag ccattggatg cctccggtca ggctgattct gtcgttggcg
      601 gcaaatgaga tgttggggtt gagcagcagg tagttgcccg atttgtagcg gatgccgtct
      661 gaaagggttt tgctgccgtt gatgcggtag gcggcggtga gggaaaggac aatcggatct
      721 atggctttgt aggtggtggc gccgatgagc cacgattttc ccgacgaggc tttgttgcgc
      781 gatttttcgt aaaccgtgct ttcaagaaag ctgattaggg cggggttttt gtcgtcttta
      841 aggaaggtgt ggctgatgcc gagggatacg tcggacatcc gtttgttgcg ggttttgccg
      901 ttgccgtcga gtttgcgttc ttcgtgccac agatagctgc cgctgccgta aatgtcggta
      961 ttgccggtca gtccgtagcg caaaccgagc gtgccggcga gcatatcggt attgctgccg
     1021 ttttcttgga tttcggtcgg aatggggata aacgaggttg cgccggtttg aatgtaaacc
     1081 ggtgcggcaa gtgcggcgcg gctgttttcg ctattcaggt aggtaaggga agtttccagt
     1141 ttccattttc ccttgtcggt cattatgtct tcaatcgtca agggcaggtc ggcataagtg
     1201 gataaaggca ggatggcggg caaggcggac aaaaagatac gtttcatatt tcctcttatt
     1261 gatattaatt cttatttaag aatagtaatg aatatccgat aattctgtct aaataaattt
     1321 ttaaatcaaa aaaaacgata tatttgtttt tatcaattat gttttgatat gccgtctgaa
     1381 aagtttgtga aaaacggtta caatccgccg catgaaaaaa cgcaataatc ctcttcctct
     1441 gttgaacggt gtcaaaccca gttatttggt gctgccgcat gaaaagcagt tttacgggct
     1501 gccgctgctg cattttctgt gcatccgctt tccttttgtg ggcgcggacg attggcgcag
     1561 gcggttgaac agcggttttg tggtcggttc ggatggtgcg acgttggacg aacatacttt
     1621 gttcgagccg ggtaaggtga tgttttatta tcgggaaacc agcagtgagg atgagccgcg
     1681 tattccgttt gaagaaaaga ttttgcatat tgatgagcat ttgattgtgg tggacaagcc
     1741 gcattttctg cccgtcatcc ccagcgggcg ttttttgagg gaaacgctgc tcacacgtct
     1801 gcgcctgcgt cctgaattgc agcatttgaa tgttgaggac attacgccgc tgcaccgctt
     1861 ggacaaggat acggcaggcg tgatgctgct gtcgcataat cctgccacgc gcggagccta
     1921 tcagacgatg tttcaaaaca aaacggtatg gaaaacgtat gaggcacttg cgccgacaag
     1981 gacggatttg ccgtatccgc tcgatgtggt ttcgcgtttg gtgaggggtg agaaattttt
     2041 tacgacgcaa gaggcggaag gcgaaccaaa cgcacatacg acggtcgaac tgattgaaaa
     2101 caggggggaa ttcagccttt accgccttac gccgcatacc ggcaagaaac accagttgcg
     2161 cgtgcatatg atgggtttgg gtatgccgct gttgaatgac gcgctctatc ccgtgccgtc
     2221 tgaagcgggc agcgaggatt atcggaaacc tttgaagtta ttggcaaaaa agattgcgtt
     2281 tgcagatcct ttgtcgggtc gggaaagggt gttttgcagt aggttttgcc tataatgaga
     2341 ggaaatgcaa acccgtatcg gcagatgccg ccgatacggg ttttacatta tcgatgccgt
     2401 cggaaagggg ttttatccgt tcaggcatac gggcgcgaag cggaggatgg tgtcgcgcag
     2461 gacgatgagt ttgccgtgaa agaaatgcgt ggaaccggcg atggtaatga cgggcaaatc
     2521 ttgcggttcc gcccatttca gggctttttc gatttcgacg acttcatctt ccgcaccgtg
     2581 tatcatcagg gttttggcaa cgttgggaac ggctgacggt tccggacggt cggtatagtg
     2641 gcagactgct gcgcccatga gcagcaataa atcgggtatg cgcgcttgtg cggcaaatgt
     2701 ggcgacataa ccgccgaagg agaagccgga taaggcaaat tcgggggctt cgggatgttg
     2761 ggcgcgggca tagtcgatga cggcgaggca gtcttgcgtt tcgccgcgtc cgtaatcgtg
     2821 tgtgccttcg ctgccgccta cgccgcgaag gttgggcagg tagcagtgga agccgagttt
     2881 gcttaaggct ttggcggcag tttggataac tttgttggtg tttgttccgc cctggagggg
     2941 gttgggatga ttgatgacgg caacaccgcg tgccagtact tgttcggacg ggatatggat
     3001 ggtttctaaa atgccggcag gaccggatat gtgtatggtt tcgggtttca gcataatgat
     3061 ttcctcttga ttttccgcat gttccgatgc agccgtgaag atagttgctt aaaggtagaa
     3121 atgcttgccg ttgttggggt tggatgcttt caggccggcg agcatgcggt ggaagtccaa
     3181 tccgtatgcg ttgctcagtt tgtcggcgcg tttcttgagt ttgtctatgt tttgctcttt
     3241 ggggctgctt tggaaggcca ttaccgcgac attgccgtgg ctttcggcag gaagttccaa
     3301 gacgcgccct tcaaaaacgc tcaacaaccg ttcgatgaag cgttggtagc gtttgtcgcc
     3361 gctccaccag ttggttacga atatgccgtc tgaagagagt gcgtttcggc agtctcggaa
     3421 gaacggttct tcaaccagcg tatcgatgat ttgttcgccg tcgaatccgt ccaccaagat
     3481 gatatcagtg ttgtggcgga agactttgat gtactctgca ccatccgctt caataatttc
     3541 aaatttctca ctctcgaaag gcaactcaaa caggttgcgg gcaatggcga taacctgcgg
     3601 gttgatatct acggcagttt gacgcgtatc aggcaggtag ttatctatcc agcgcgcaaa
     3661 cgagccgccg cccaagccga tttgggtgat atgttgcgga agagcgtcgg taaacagcag
     3721 ccagcccatc atcgcgcggc tgtaagagag caccagctcg gacgggtggt cgaggttcat
     3781 cgagctttga acggtgtcgc tgcccaagtg aagcgaacgg atattgcctt cttcggaaat
     3841 gccgacttcg ggaaagccgg attttgcagg acgcaggcgg cggtagggat gtcttgccat
     3901 cggtgtgaac ggtcggtttg aaaggcggat attttatcag aatgccgatg cttgttctgt
     3961 ttcaaattaa tttcttttca aataaattac ttattcggat ttgccggggc tttcggataa
     4021 attccttgcc aaggtgcggc attgcctgca taattcgctt tctttgccgg tatagctcag
     4081 ttggtagagc acctgacttg taatcagggg gtcccgagtt cgactcttgg tgccggcacc
     4141 atttctgctg atggtgttgc aaaacatttc agtaggccgg tatagctcag ttggtagagc
     4201 acctgacttg taatcagggg gtcccgagtt cgactcttgg tgccggcacc agatttgaca
     4261 gtccgattgt gtaaaacagt cgggctgttt tgcatttgcg gcacggtcgg tggcagggct
     4321 gcgggcattt cactataata gcccgtttca aactgaaaag gccgtctgaa aagggcgggg
     4381 taacaatatc tgatgattac tgtgaacaca ctgcaaaaaa tgaaggcggc gggcgaaaaa
     4441 atcgctatgc tgaccgctta cgaatccagt tttgccgcgc tgatggacga tgccggcgtg
     4501 gaaatgctgc tggtcggcga ttctttgggg atggcggttc aggggcggaa atcgacgctg
     4561 cccgtcagcc tgcgcgatat gtgctatcac accgaatgtg tagcacgcgg tgcaaaaaat
     4621 gcgatgattg tcagcgattt gccgtttggt gcatatcagc agagtaagga gcaggcgttt
     4681 gccgccgccg ccgaactgat ggctgccggc gcgcatatgg ttaaacttga aggcggtgtg
     4741 tggatggcgg aaactaccga attcttgcaa atgcgcggta ttctggtttg tgcgcatatc
     4801 ggtctgacgc cgcaatcagt gtttgccttt ggcggatata aggttcaggg tcgcggaagt
     4861 aaggcgcagg cgttgcttaa cgatgccaag gcgcatgacg atgcgggggc ggcggtcgtg
     4921 ttgatggagt gtgttcctgc ggaactggca aaaaaggtaa ctgaatccgt ttcttgtccg
     4981 accatcggca tcggcgcggg ggtcgattgc gacgggcagg ttttggtgat gcacgatatg
     5041 ctcggtattt ttccgggcaa gacggcgaaa ttcgtcaaaa actttatgca cgggcaaagc
     5101 agcattcagg cggcggttcg ggcgtatgtt gccgaagtca aagccaaaac cttccctgct
     5161 gcggaacata tttttgcaga ctgaacaagg tttgctgctg atgccgtctg aaagccgttt
     5221 cagacggcat tttgttttgc cgtgcgcggc gcgtataatc ggcgcgtttt gtcgggcagg
     5281 aagcccgaag gataaggatt accgttatgc aaatcataca taccattcga gaactgcgcg
     5341 cgtggcgtaa aaatgcggga aaggtggcat ttgtgccgac catgggcaat ctgcatgaag
     5401 gacatcttgc gcttgtgcgt gaggcgaaaa aacgcgcgga cagtgtcgtg gtcagcattt
     5461 tcgtcaaccg cctgcaattc ggtcagggcg aggatttcga caaatatccg cgtactttgc
     5521 aacaggatgc ggacaagctg gaagccgagg gtgttgccgt tgttttcgcg cccgatgaaa
     5581 aagaactcta tccgaacgtg gagcaacggt ataacgtcga accgcccaac ctgcaaaatg
     5641 agttgtgcgg caagtttcgt ccgggacatt tccgcggggt ggcgacggtg gtgtccaagc
     5701 tgttcaatat tgtcgcgccg gatgttgcct gtttcggcaa aaaagactat cagcagcttg
     5761 ccgtgattaa aggtttgacc gaggatttga attttgatgt tgaaatagtg cctgtcgata
     5821 cggggcgcgc ggaagacggg ttggcactgt cgagccgcaa ccagtatttg agtgcggcgg
     5881 aacgcgacga agcaccgcgc ctgtaccgcg aattaaaggc tgttgccgaa tccttggcgc
     5941 agggcagttt ggattatgca ggtttggagc ggcaggctgc cgaccatttg accgctgcgg
     6001 gctgggtggt cgattatgtc gaaatccgcc gcgccgatac gctcgaagtg gcgcgggcgg
     6061 gagataagaa actggtggtc ttggccgccg cctgtctggg gacgacgcgc ctgattgaca
     6121 atttggaaat aaaactccct taaaccgcaa gcgtcgggaa tgccgtctga agcggatttg
     6181 c
//
LOCUS       DAXHRI010000118         6150 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXHRI010000118 DAXHRI010000000
VERSION     DAXHRI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.118"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(441..2094)
                     /locus_tag="ACFM75_001622"
                     /pseudo
     CDS             complement(441..2094)
                     /locus_tag="ACFM75_001622"
                     /inference="COORDINATES: protein motif:HMM:NF017826.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
     gene            complement(2213..3265)
                     /locus_tag="ACFM75_001623"
     CDS             complement(2213..3265)
                     /locus_tag="ACFM75_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFC6256136.1"
                     /translation="MKPTYQDIKAAARYRWQEIHAAIGIDPKFLRNKHQPCPACGGED
                     RFRYDDKDGKGTFICTHWESGAGDGFGLVMHFLGCDFQTALKQVSGILGMDETNPLPI
                     PTTRPQPQPRPEKDYIGKLAALWDGAEPLTPDCPVVQYWTSRGLNAAYLPENIRHHAG
                     MEYWTQGEDGKPLFIGRFHAAIAASTRGSTVCGLHITYLEAQRGGGFAKARIKHPQTG
                     EDLPAKKMRSRKQGAMTGGAVYLFPVPENGRLMVAEGIETALAARELFNAHDWGLCAA
                     LSANGMKRLELSDGIKELAVIADHDAPRPVGETAALSLAKRAQSGGIAVRYWISGTAG
                     YDALDELNDRKRSGKA"
     gene            complement(3262..3483)
                     /locus_tag="ACFM75_001624"
     CDS             complement(3262..3483)
                     /locus_tag="ACFM75_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256137.1"
                     /translation="MRQDNRPRKTPYAGAAVFLIFGFAFAGMTHHAPENHTADIPPPV
                     ETQPQTPAIWETDPMAGVVLEPVSEEAEK"
     gene            complement(3637..3999)
                     /locus_tag="ACFM75_001625"
     CDS             complement(3637..3999)
                     /locus_tag="ACFM75_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256138.1"
                     /translation="MQCDFSQAVPSNQTQNIQNQAQTVTIPHKLANDAADACFYADRA
                     SENLAAILEAVEALIDISTTTQTGQAQRHNLPLIARNLIKTAKNEVETLAEFHPFSLF
                     ETATDLNNEIFSQVHSAK"
     gene            4401..5054
                     /locus_tag="ACFM75_001626"
     CDS             4401..5054
                     /locus_tag="ACFM75_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6256139.1"
                     /translation="MKAVFVELPPFERFRADYFSSEEYRAFQNELIANPEKGDVIQGT
                     GGLRKIRVADSKRGKGKRGGSRVIYYWAVSRAVFLLFTVYGKNVKDDLSPSEKRYFPP
                     CFITSRKITMSERNLFEELKEGLEAAEQYLQGKITLRAIEAEIPENGAAAISPEEIRA
                     IRESLNFSQAVFARKLRCSVRTYQGWEQGKTRPNPQAALLLRMVQKSPKTFKTIAAL"
     gene            complement(5049..5303)
                     /locus_tag="ACFM75_001627"
     CDS             complement(5049..5303)
                     /locus_tag="ACFM75_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6256140.1"
                     /translation="MKHDCINDKPKTPNQRQRILARLRQGSVTSWELTQMGILCYNTR
                     IMELRQQGHNIVTTMEEIQNQFGETVKRGRFSLVSEGRQS"
     gene            complement(5365..5580)
                     /locus_tag="ACFM75_001628"
     CDS             complement(5365..5580)
                     /locus_tag="ACFM75_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6256141.1"
                     /translation="MDQSVLRVNQVAERLGVSRSTVWNWGNTKSRHYRADFPRPFKVS
                     ANVVGWFSSEIDDYIGKLAAKREEQAG"
     gene            complement(5734..>6150)
                     /locus_tag="ACFM75_001629"
     CDS             complement(5734..>6150)
                     /locus_tag="ACFM75_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256142.1"
                     /translation="AIFRMNDIAPKDTTIMQDVLIGWTSEERVFSLRQTIQAEEQARR
                     EAAKAEAEKQEVRALPYCVIPEFETPEFETACLVVRDFWNRQAVGVAPPKAIIIQEFI
                     KNTFKNITGVEPKEAAIRRIDSLTRPPLFKNQSKKQ"
BASE COUNT         1263 a         1573 c         1574 g         1740 t
ORIGIN      
        1 agtttaaatt taatccacta tatttagtct tatctatttc attaaacagc aatagacaaa
       61 aaaataaccg ctctaaaagc ggttgtcaga atctctctcg aaatgaaagc atatcattct
      121 gatttaaata tattttattt ttagccaaat caataatacc gtaaaaaata ccgtaaattt
      181 gtcaaagtca aaatttttca gggacggttt gtggcatttc tgccaagctg tgaaatatgg
      241 gcacattgta acatcggcaa tcatgcgccg ccccgtataa aaaaacagta tatatataaa
      301 aatcacttgt taccgttgtt actcttgtta ccgcttcgca acattctgtt tttcggcttt
      361 tcctgtgccg tgatagcctt tttctttcgt tttcctgctc tcgatagcgt aaaaaagccg
      421 cctttcggcg gctctctgta tcaatcaagt tctagcggct caatgattaa ataatggcgt
      481 atcggcttct tgttcacttt gatttggtgc tggtagcctg ttttggacgg cttcaagcgt
      541 ccttttcccg atagtacctt acacacggta tgaatcggga acgattgggc gatttcttct
      601 ctaaacacca gcggcaggat ataaaactcg tcattgccac cagcctgcac tttaaagcct
      661 gcgtgccctt tgcctttgcc gcctgtcatg gtgtatggcg cgtctctttc gctccacgac
      721 aggaagcgcg gggaatcggc atattgtgcg ataaagtcgt ccaactggct gcatatcgct
      781 tctgcgtcgc ggtctcctgt gccgttctct tccagccatt cgtgaaagca ctgcataacc
      841 gccgccccgc ctgcctttaa tcctgtgatg ggcgcggcat attctaaaac ggcggctaaa
      901 agggtaaaac ggcgggctat cctccgtgcc tgcccttcca taggcggcaa ggcttcaagg
      961 taccgggcgc gtgttgcttc tatggtttcg gcggcggctt ctttgccgtc tgaaaggatt
     1021 tggcggataa gggcgcgccc tgccgtgccg tatttcttgg cggctgccct gttgatatgc
     1081 tccgacagtt tcgcgccgtc tgtgaagccg tgcagcgtgt cgtatatgcc gtgtttggct
     1141 tctgcctgta tatcgggcaa tcgggcgcgg ttacctgccc tccattgggc gcggtctccg
     1201 attaggcttt cggggttggt ttcgcccgtt gagagtatca aattgcgcca tgtcttttgt
     1261 ttgcggttgc caccgtcttt cgcgccctgt gttttcgata tgccgttaat cacgctgtaa
     1321 accatttggg ggattcttcg cgggtctgcg gtctgtccga tttcgtccag tgctaaaaag
     1381 ccgtcgttac gcgctaaggc ggcattctgc aaacctaatg cggtagtgtc ccagttgctt
     1441 tttgtggctt catagctgcc ccatacgctt aatgcaaggt tggcggctgt ggttttgcct
     1501 ttggacgaac tgcccgccaa gtgtacgccg ccattcttca aggctgcccg attccgtata
     1561 gccctccctt tggctggtgt ctccgttgta taggatgttt gcgccctctg cgttgatggt
     1621 ttcgccattg ggcaggatat aggcgttgcc gtgccagcct gcgcggtcgg taatggtgta
     1681 aatatcgccg ctgccctgtg tctgcaaata gtcggaaagc cgctcacgct taaccctgcc
     1741 cgataagaca gccaagccgt aagactgcaa acgctgccag ccttgaactg taccgatttc
     1801 cgattgcggg atggcggcga ttttggttcg ccgtgtgtta gcgtcctgcc agcgtatcac
     1861 gcggtaatat gcgccgtcgt tgtccgtgcc cctgcctatg atttcaatgg gggatgagag
     1921 ttttaccggc gtggcttcct gcgtgttgcc ctccctatcg gtcttaacgc cgatatacca
     1981 cacgcccgcg ccgtccacgt cgtaacgcgg ttgtgcaaac ggcgcggcaa attccgacat
     2041 atcgcccaat ggggcggctt cttctgcgaa attcggcttt acatctttac acatagtgtt
     2101 aaaatctcct gtatgggttt ggttgccttt cgcggttttc cgcgtttggt ttccgtttga
     2161 taaataatcc attgccgcct acaaatgccg cctgtttttc agacggcatt ttttacgcct
     2221 tgccgctgcg tttgcggtcg ttcaattcgt ccagcgcgtc atagcctgcc gtgccgctta
     2281 tccagtatcg gacggctatc ccgccacttt gggcgcgttt tgccaggctt aatgccgccg
     2341 tttcccctac ggggcgcggt gcgtcgtggt ctgcgataac ggcaagctct ttaatgccgt
     2401 ctgaaagctc cagccgcttc atgccgtttg cgcttagggc ggcgcataag ccccaatcat
     2461 gggcgttgaa cagttcacgc gcggctaagg cggtctctat gccctccgct accatcagcc
     2521 gcccgttttc agggacgggg aacaggtaaa ccgcgccgcc cgtcattgcg ccctgtttgc
     2581 ggcttcgcat tttcttggcg ggcaaatctt cgcccgtttg ggggtgctta atccgcgctt
     2641 tggcaaagcc gccgccccgt tgtgcttcca gataggtaat gtgcaaaccg caaacagtgc
     2701 tgccgcgtgt ggaagccgct attgccgcgt ggaaacgccc gatgaacagc ggtttgccgt
     2761 cctccccctg cgtccaatat tccatgcctg cgtgatggcg gatgttttca ggaaggtatg
     2821 ccgcgtttaa gccgcgtgat gtccaatact gcacaacggg gcaatcgggc gttaacggct
     2881 ctgccccgtc ccacaatgcg gcaagtttgc cgatatagtc tttttcgggg cgtggttgag
     2941 gctgtgggcg cgttgtcggt atcggtaagg ggtttgtctc gtccatgcct aaaatgcccg
     3001 aaacctgctt taatgccgtc tgaaaatcgc agcctagaaa gtgcataacc aagccgaaac
     3061 cgtcgcccgc tccgctctcc caatgggtgc aaatgaatgt gcctttgccg tccttgtcgt
     3121 cgtatctgaa gcggtcttcg cctccacacg cggggcaagg ctggtgtttg ttgcgtaaaa
     3181 acttagggtc tatgccgatg gcggcgtgta tctcctgcca gcggtaacgc gcagcggctt
     3241 tgatgtcttg gtaagtcggt ttcatttttc cgcctcctcc gatacaggct ctaacacaac
     3301 accagccatc gggtcggttt cccatattgc gggcgtttgc ggctgcgtct caacaggggg
     3361 cggaatatcc gccgtgtggt tttcgggggc gtggtgcgtc atgcctgcaa aggcgaaacc
     3421 gaaaatcagg aaaaccgccg cccctgcgta tggcgttttg cgcggtctgt tgtcttgtct
     3481 catggctttt tctcctgtgc cgatgtttgg aataagccta aacccgaaac ctaaaacaca
     3541 tataaatcaa tgtgttatct aaaacatagg tttgagtttg cccctgctgc ctaaaaatag
     3601 gcggcattgc ggtttaggtt ttacggggtt tatggtttat ttcgcgctgt gaacttggct
     3661 gaagatttcg ttattcaaat ccgttgcggt ttcaaacagg ctgaaagggt gaaactctgc
     3721 cagtgtctct acttcgttct tggcggtttt gatgaggttg cgggcaatca gcggcaaatt
     3781 gtgccgttgc gcctgccctg tttgcgtggt agtggaaata tctatcagtg cttccaccgc
     3841 ttcaaggatg gcggcaaggt tttcagacgc gcggtctgcg taaaagcaag cgtctgctgc
     3901 gtcgtttgcc agcttgtggg ggatggttac cgtttgggct tggttttgaa tgttttgggt
     3961 ttggtttgat ggtactgctt gagaaaaatc gcattgcatt tggaatgctc ctatttgttt
     4021 tgagatttga gaatgcccga gaaggggggc ggtgctctct cctgcaaata gtcaggcgtg
     4081 ggcgttgccg ctaccacaca ccgccataac ttggatatgc cgtctgaagt cttgaacaga
     4141 ctgcaaacag cataggggaa atggcgataa aaaaattccg caagccgtgc ggaatttcgg
     4201 ctatttgttt agagagaaac cgcaatctag tttctcaatc ccgatttgta aatacttttc
     4261 agacggcatt tttacaagcc gctgattcca ttgactatta aatcgcgtta taccgcgttg
     4321 ccgtacattg gaagccgtgc ggcgttgcct ttctatacgc tttgcgcgta tgatttggtt
     4381 ttcattcaaa ggggctttta atgaaagccg tatttgtcga actgccgccg tttgaacgtt
     4441 tccgtgctga ctacttcagc agtgaggaat accgcgcctt tcaaaatgaa ttaatagcaa
     4501 atcccgaaaa aggcgatgta atacaaggga cgggcggtct gcgtaaaatc cgcgttgccg
     4561 attccaagcg cggcaagggc aagcggggcg gctctagggt tatctactat tgggcggtca
     4621 gccgtgccgt tttccttttg ttcactgttt acggcaaaaa cgttaaagat gatttgtctc
     4681 caagtgaaaa aagatacttt ccgccatgct tcataacctc aaggaaaata accatgtctg
     4741 aacgcaattt gtttgaagaa ctgaaagagg gcttggaagc cgccgaacaa tatttgcaag
     4801 ggaaaatcac attgcgggca atagaagcgg aaatacccga aaacggggcg gctgccattt
     4861 ctcctgaaga aatacgcgcc attcgcgaaa gcctgaattt ctcccaagcg gtctttgccc
     4921 gcaaactccg ttgctccgtc cgtacatatc agggctggga acaggggaaa acgcgcccga
     4981 acccccaagc cgccttattg ctgcgtatgg tgcaaaaatc gcctaaaacg tttaagacga
     5041 ttgccgcgct atgattgcct gccctccgac accagcgaaa accgcccgcg cttcacggtt
     5101 tcgccaaatt ggttttggat ttcctccatt gtggtaacga tgttatgccc ctgttggcga
     5161 agctccataa tgcgcgtgtt gtagcaaagt atccccattt gggtaagctc ccatgaggta
     5221 acgctgccct gcctcaatcg ggctaaaatg cgctgtcttt ggtttggtgt tttgggcttg
     5281 tcgttgatac aatcatgctt catcattgat tcccctttcc gccgcctgaa tgtccgtttt
     5341 ggcggctttg ttttgcccgg taaattaacc cgcctgctct tcgcgtttgg ctgcaagttt
     5401 gccgatatag tcgtcaatct cactggaaaa ccagccaacg acgtttgcgg atactttgaa
     5461 cgggcgcgga aaatcggcgc ggtaatggcg gcttttagta ttgccccaat tccacacggt
     5521 cgaacggctc acgcctaaac gctcggctac ctgattaaca cgcaatactg attggtccat
     5581 gctttcgctc ctatttggtt gtgttggtac ggcgtgaatt gtcagatagc cgtatagggt
     5641 ttaggcaata aaaaaacggt taaaccctcc tttcaagaaa ggggtttaac cgtcctgtaa
     5701 tgtatgattg ataagggctt gggggttggc tgcttattgt tttttagatt ggtttttgaa
     5761 taacggcggg cgcgtcaaac tgtcaatgcg cctgattgcc gcctccttgg gttctacccc
     5821 tgttatattc ttaaaggtgt tcttaataaa ctcttgaata ataattgcct tagggggtgc
     5881 aacccctacc gcctgcctgt tccaaaaatc cctcaccact aaacaggcgg tttcaaattc
     5941 aggggtttca aattcgggaa taacgcaata aggcaatgcc ctcacttcct gtttttctgc
     6001 ctctgcttta gccgcttccc gccttgcctg ttcttctgcc tgaatggttt gcctcaagct
     6061 aaacacacgt tcttccgatg tccagccaat tagcacatct tgcataatgg tcgtgtcctt
     6121 tggtgcgatg tcattcattc taaaaatagc
//
LOCUS       DAXHRI010000119         6136 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXHRI010000119 DAXHRI010000000
VERSION     DAXHRI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.119"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(170..2098)
                     /gene="dnaK"
                     /locus_tag="ACFM75_001630"
     CDS             complement(170..2098)
                     /gene="dnaK"
                     /locus_tag="ACFM75_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC6256143.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIQRSIEPCKVALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGEVLGGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2306..2578)
                     /locus_tag="ACFM75_001631"
     CDS             complement(2306..2578)
                     /locus_tag="ACFM75_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256144.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2764..3450
                     /locus_tag="ACFM75_001632"
     CDS             2764..3450
                     /locus_tag="ACFM75_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6256145.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3582..3920
                     /gene="erpA"
                     /locus_tag="ACFM75_001633"
     CDS             3582..3920
                     /gene="erpA"
                     /locus_tag="ACFM75_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC6256146.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3937..4083
                     /locus_tag="ACFM75_001634"
     CDS             3937..4083
                     /locus_tag="ACFM75_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256147.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4080..4544
                     /locus_tag="ACFM75_001635"
     CDS             4080..4544
                     /locus_tag="ACFM75_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6256148.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPTRNIKPLVFGLLIAFLGYLLGKIF"
     gene            4579..6090
                     /gene="ubiB"
                     /locus_tag="ACFM75_001636"
     CDS             4579..6090
                     /gene="ubiB"
                     /locus_tag="ACFM75_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC6256149.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPVIEQ
                     DLSLMRFGAGWVERLFADGKRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1405 a         1355 c         1619 g         1757 t
ORIGIN      
        1 aatttaatcc actatataaa aaatttccag agaaccgata caacagttgg aacttgggtt
       61 tgggaatatt acggtagatg aacttggaac ctctgttatg ctatggtctt ttatctcaat
      121 tgaaaaaagc gcgaatcaaa cggttcgcgc ttttttcaga cggtattaat tattttttgt
      181 cgtcttttac ttcttcaaag tcggcatcta cgacatcatc gtctttcttt gcagaagcat
      241 tggcttgttc gctttcgcct gcttgggctt cagcttgtgc ttgagcgtaa accatttccc
      301 ccagtttttg gctggctgcg cccagcgcct cggttttggc atcgatagcg gctttgtcgt
      361 cgcctttaac tgcttcttcg gcttctttca gcgcggcttc gattttttct ttctcggctg
      421 cgtcgagttt gtcgccgtag tcggccaaag attttttcac agagtgaatc agggcttcgg
      481 cttggttgcg ggaagcgacc aattcagtca gttttttatc ttcctcggca ttggcttcgg
      541 catctttcac catgcgttcg atttcttctt cgctcaaacc tgaagaacct tggatggtga
      601 tgttggctgc tttgccagtg cctttgtctt tggcggaaac gtgcaggata ccgttggcgt
      661 caatatcgaa ggtgacttcg atttgcggca taccgcgcgg tgcgggtgcg atgtcgccca
      721 agttgaactg acccaaagat ttgttggcag aagcgcgttc gcgttcgcct tgcagtacgt
      781 ggatggttac tgcgctttgg ttgtcttcgg cggtagagaa cacttgcgac gctttggtcg
      841 gaatagtagt gttcttttga atcagcttgg tcatcacgcc gcccatggtt tcgataccca
      901 aagacagagg tgttacgtcc agcagcaata cgtcgctgcg gccgccgccc aatacttcgc
      961 cttgaatcgc tgcacctacg gcaacagctt cgtcagggtt cacgtcttta cgaggctctt
     1021 taccgaagaa ggctttaacg gcttcttgta ctttcggcat acggctttga ccaccgacta
     1081 agatcacgtc gtcgatgtcg ccggtgctca agccggcatc tttcaatgcg actttacaag
     1141 gctcgataga acgttggatc aaatcttcaa ccaggctttc aaatttggca cgggtaattt
     1201 tcattgccaa gtgtttcgga ccggttgcgt ccatagtgat gtatggcagg ttgatttcag
     1261 tttgctggcc gctggacaat tcgattttgg ctttctcagc agcttctttc agacgttgca
     1321 gagccattac gtcttgtttc aaatcaatgc cttgttcttt tttgaactcg tcaatgatgt
     1381 agtcgatcaa gcgttggtcg aagtcttcgc cgcccaagaa agtatcgccg ttggtggcca
     1441 atacttcaaa ttgtttgtcg ccgtcgaggt tggcgatttc gatgatggag atatcgaaag
     1501 taccgccacc caagtcatat acggctactt tgcggtcttt gttgtcgcct ttgtccatac
     1561 cgaatgccaa agctgcagcg gttggttcgt tgatgatacg ttttacttcc aaacctgcga
     1621 tgcggcccgc gtctttagtc gcttgacgtt ggctgtcgtt gaagtaggcg ggaacggtaa
     1681 tcacggcttc ggttactttt tcgcccaagt aagcttcggc ggcttctttc attttacgca
     1741 aaacttctgc ggaaatttga ggaggagaca gctctttgcc ttgcgctttc acccatgcgt
     1801 cgccgttgtt ggctttgatg atttcgaaag gcatggattc gatgtcgcgt tggacttctt
     1861 tgtcttcaaa tttgtggccg atcaggcgtt tggcggcgta aatggtgttt ttagcgttgg
     1921 ttacggcttg gcgtttggca ggcgcaccga cgaggatttc gccgccgtcc aaataagcga
     1981 taacggacgg cgtggtgcgt gcgccttctg cgttttcgat cactttggtt tgaccgtttt
     2041 cggaaatggc caaacaagag ttggttgtac ctaagtcgat accgattact tttgccatgt
     2101 ggataatcct atttgatttt gcttattttg agaaatatgt tggaacattt tgtcccgatg
     2161 ggctgtaaat agggcgggcg gcgggctgtt tcaagctaca gcatggctat aagtatataa
     2221 ctttatgaat atattggttt tatatttgat ttaatacatt tggctccaat gcattcaagc
     2281 ataatgtttc aaatggcagg caggtttatt catagacgat gccggcgagc atttcctgtt
     2341 cgttcaagtt gccgtactct ttttcccagt cgtgtgaaga ctcgatgatg tcgcattctt
     2401 tggaaaggga gacttgttct gcatccatat ctttggcgtt cagtatgttg aattgttcgc
     2461 acagggatgc ggataaagtg atgtcgggct gtttggcttc agaacggttt tcttggaagg
     2521 caaagcagaa tgcggtaaat gccgcagcat agataagata tttgccggtt ttcttcattt
     2581 tctatccttt ttctgtcaac tcaggattaa acctatggaa aatcttaaaa attatgtatt
     2641 gagtaagaaa aatcataatt taaatttagt ttatcatagt tgttccgttt ttgggatagc
     2701 taaggtaaaa tatatttcat gtttacttta gatgattgaa tgaaggggag cggaaggata
     2761 tttatggaaa cctttaaaga cagactggtt tttttatgga aaagcgaagc gaagcaggca
     2821 aaaatcgcat ccgatattga aatgacgatt gcgggcttca gcaggatatg gaatgaaggc
     2881 ggtctgccga agtctgaaac attaaaaaaa atcaagcagt tgaaggggtg tagtatcgat
     2941 tggctgctga ccggggaggg taatccgttt ccggatgaag ccccaaaaaa atcccttgct
     3001 tacgatacct tgggaaatga agtcgatacg gacgagtttg tctttgtgcc gagatatgat
     3061 attcgggcgg ctgcgggata cgggcagttt gtcgatcatg aggaaccggt atttacaatg
     3121 gcgttcagac ggcattggat tgagaattat gttacccgcg atacgaaaaa cctgtctgta
     3181 atttccgtca agggggattc gatggagggg gttttgaatg acggcgattc gattttggtc
     3241 aatcatggtg aaaatacgcc gagggacggt ctgtatgtgt tgcggattaa tgaaaatctg
     3301 ctggttaaac gtttacagat tgtaccgggc gggattatca atgtgatttc tgcaaacgag
     3361 gcttatcctg cttttgaaat caatttgaac gatttgaccg atgatgtgga gattatcggg
     3421 cgtgtcgagt ggttcggcag gacgatttga gtttggggct tgaaattgca ggcggtcaaa
     3481 cttatctatt ggaacaattc ctttttcaaa ggcgaagcct gcttgccttt gaagggggtt
     3541 tgagagagaa tgcagaaaat attatattaa ggaataacac catgtcggat gaaagcccta
     3601 ttatttttac tgacagctgc tgtgccaaag ttgccgattt gattgccgaa gaaaacaatc
     3661 ccgatttgaa attgcgggtt tttgtcaatg gcggcggctg ttcgggtttc cagtacggat
     3721 ttacttttga cgaaatcaaa aacgacgacg attttgaaat tgagaaaaac ggtttggtct
     3781 ttttggtcga tccgatgagc tatcaatatc tggtcggtgc ggaaatcgac tatacggaaa
     3841 gtttgcaggg ttcgcaattc gtcatccgca atccgaatgc ggaaacaacc tgcggttgcg
     3901 gatcgtcgtt ttccgtatga ccgcttggtt tgtgtgatgc cgtctgaacg ttcagacggc
     3961 atttttactt ttagaaaata tattatcggg atgaattcac atataatccg attgtttgaa
     4021 gatgaatcgg gtttcccgaa aggaacgggc ggaacggtat caggcgtatt tgttccctta
     4081 tgattgagat gagtaaagat taccgaaacg atttgtacga tgtatatgtt tcttacccgc
     4141 cccaagtgga tcgcgggctt atccgggagt gccttaagga gaatctcggc gaggaaaagg
     4201 cggaaggatt gatcgaatcg ctcgattcca aacctcaagt gctggttgag gaaaaatgca
     4261 cttgggcgaa acgggaagag ttgcatgatt atttcagcta tttgggtttg gatattatta
     4321 cccggagata tatggagttg gaaacggtcg tgccgccgga ggaaggtgag ggcgaaggag
     4381 agggcgcgga tggggaaatg cccgaatatc ttgaacttca tggcgggcgg gaagatgata
     4441 tttccgcacc ttcgcaaccc gaaccgccga cccgcaatat caaaccgctg gttttcgggc
     4501 tgctgattgc ctttttgggc tatctgctcg gtaagatttt ttgattgtcc gataaatgct
     4561 gtattcggga ttttatatat gaaatggttg aaacgcctga cggtcattgt cgggactttt
     4621 taccgctatc ggctggcagg tctgtgtgct tcgctgatgg gtagcggttg gatatgcgct
     4681 ctgctgaaaa tgatgccgca gtcgtccaaa ttgaaaaacg aaccgcctgc tgtccgtctg
     4741 cgccttgcct tggaaagcct ggggccgatt ttcatcaagt tcgggcaggt attatctacg
     4801 cgccccgatt tgattccgca cgattacgcc gtcgaactgg caaagctgca agacaaagtg
     4861 ccgccttttg acgcgcggct ttcgcgtgaa caaatcgaaa aatcgctggg tcagcccatc
     4921 gaaaagctgt atgcggaatt tgaaaccgag cccgtcgcca gcgcgtccat cgcccaagta
     4981 cacaaagccc gcctgcattc gggcgaacga gtggcggtca aagtcttgcg ccccaacctt
     5041 ttgcccgtga tcgaacagga tttgtcgctg atgcgctttg gtgcaggctg ggtcgagcgt
     5101 ctgtttgccg acggcaagcg tctgaagccg ctcgaagtgg tggcggagtt cgacaaatac
     5161 ctgcacgacg agttggattt gatgcgcgaa gccgccaatg ccggacagct cgggcgcaat
     5221 ttccaaaaca gcaatatgct gattgtgccc aaggtgtttt acgactactg caccagcgac
     5281 gtgctgacaa tcgaatggat ggacggcacg ccggttgccg acatcgccaa actcaaagca
     5341 gacggcatcg atttgcacaa actcgccgat tacggcgtgg aaatcttttt cacgcaggtc
     5401 ttccgcgacg gctttttcca cgcggatatg caccccggca atattttggt tgccgccgac
     5461 aaccgctaca tcgccctcga tttcggcatc gtcggcacgc tgaccgatta cgacaaacgt
     5521 tatctcgcca tcaatttcct cgcctttttc aaccgcgatt accggcgcgt cgccaccgcc
     5581 cacatcgaat cgggctgggt gcctgccgac acgcgtgcgg aagaattgga agccgccgtc
     5641 cgcgccgtgt gcgaaccggt gttcaacaaa ccgatttcgc agatttcctt cggcttggtg
     5701 ctgatgcgcc tgtttgaagt cagccgccgc ttcaatgtcg aaatccaacc gcagcttgtg
     5761 ctgctgcaaa aaacgctgct caacatcgaa ggcttgggcc gccagctcga tcccgatttg
     5821 gacttgtgga aaaccgccaa accgtttttg gtgaaatgga tgaacgggca ggtcggccct
     5881 aaagcccttt ggcgcaacct caaaaacgaa gcccccgact gggcgcaaat cattccttca
     5941 ttgccgcgca aaatcagtgc gttgattgat gaaaaccgcc agcaggaaat gcgtgatgcc
     6001 tatatccatt tggtcaaagt gcagcagcgg caaagcctgt ggctggctgt gattgcggtt
     6061 gttttgctgc tgattttgct tttgaaatag gctttgtccg aatcatcgcc cgactccgcc
     6121 cgtttataag gaaatc
//
LOCUS       DAXHRI010000120         6122 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXHRI010000120 DAXHRI010000000
VERSION     DAXHRI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.120"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(1043..3793)
                     /gene="tbpA"
                     /locus_tag="ACFM75_001637"
     CDS             complement(1043..3793)
                     /gene="tbpA"
                     /locus_tag="ACFM75_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC6256150.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDGSSDYVYFVVEEECKSGSYETCKSK
                     PKKDIVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEH
                     TQQTFDTRDMTVPAFLTKAVFDANKKQAGSLSGNGKYAGNHKYGGLFTNGENGALVGA
                     EYGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSAD
                     GSDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFG
                     SDLRHQDYYYQNAKRAYSSKIPTQNGNQKISPNGSQDKPYWVSIGGGNVVTGQICRLG
                     NNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDSSVSTGTHRT
                     LSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAG
                     IVFKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINVL
                     GKIDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGY
                     DQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYT
                     IKKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEM
                     KF"
     gene            complement(3880..5946)
                     /locus_tag="ACFM75_001638"
     CDS             complement(3880..5946)
                     /locus_tag="ACFM75_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6256151.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPKAQKDQGGYGFAMRFKRRNWHPRVNPKEDKIELSENDWEQTDNGNIKTPS
                     KQKNIINALLGSEGALLQDSSQEGQSISKVTDHHNFQYVWSGFFYKHIQTKFETTGGK
                     MTARSGPDGYIFYKGKDPSRKLPVSGEVMYKGTWDFLTDVKANQKFTDLGNASTKPGD
                     RYSAFSGELDYIVKQENDKKDGHVGLGLTTEITVDFEKKTLSGKLIKNNSVITTNNDK
                     HTTQYYSLEATLKGNRFNGKATATDKPKENETKQHPFVSDSSSLSGSFFGPKGEELGF
                     RFLSDDKKVAVVGSAKTKDKNANGNTPAASSGAGAATMPSETRLTTVLDAVELKSDGK
                     KVENLDNFSNAAQLVVDGIMIPLLPKDSEIGSNQADKGKNGGTDFTRKFDHTPKSDEK
                     DTQAGTAANGNQAASNTAGDANGKTKTYAVEVCCSNLNYLKYGLLTRKTAGNTVGSGN
                     GSQAAAQTDAQSMFLQGERTDEKEIPNDQNVVYRGSWYGHIANGTSWSGNASNENGGN
                     KAEFTVNFAEKKLNGTLTARERTSPTFTITATIQDNGFSGTAKTADLGFDLDQSNTTG
                     TPKAYITDAKVQGGFYGPKAEELGGWFAYPGDKQTEKATVASGDGNSASSATVVFGAK
                     RQQPVQ"
BASE COUNT         1315 a         1554 c         1510 g         1743 t
ORIGIN      
        1 gtcgttccca cggtcaggat ttcagattgc ggacatctgt cagaaaagac aaaaaacctt
       61 ccgccgtcat tccctacagg cgagaatccg atccgttgaa attcggttgt tttaaataaa
      121 ttcttgcagc tttgattttc tgtttttccg ataacgccgt aactttgaaa cgcgaaagcg
      181 gtaatccgat ccgttgggat tttgcaactt caaatcaatc cgcaaactga aatcccgtca
      241 ttcccgcgca gtcgtgaatc cgaacgcgtc cgcacgaaaa cctgcatccc gtcattccca
      301 cggaagtggg aatctaggac gtaaaatctc aagaaaccgt tttacccgat aagtttccgc
      361 accgacagac ctggattccc gcctgcgcgg gaatgacgaa atttcagcga gccgtagggt
      421 gggctgtagg gtgggcttca gcccaccaat ttcaccgcat caaaggtttt tgggaatacg
      481 gcggtttcgg ttttcggtgg gaatgggcgg aatggataac attgacggag ttgggggaat
      541 aggtggaaac ggtgggattg gtgggctgaa gcccacccta cagccagccc tatgtccacg
      601 ctgcacatcc tacacccgcc ttgcgtttct tataagcagc attttacgca atactttgcg
      661 tccgctgaaa aaatttcaaa cggtcttttt tatcaaatgc gaaaaatata acgtcattcc
      721 cgcgcaggcg ggaatctagt ctgtcggtgc ggaaacttat cggataaaac ggtttcttta
      781 gattttacgc cctagattcc cgcctgctcg ggaatgacgg gatttgaggt ttctgttttt
      841 gattttctgt tttcgtagga atgacggttt agagttaccc gaaacctcaa aaaaaaccga
      901 aaccgaacgg actggattcc cgcctgcgtg ggaatgacgg gtttcaagat tacggtattg
      961 tcggggatga tgggaaacgg tgggaattgt gtaaaaaatg ccgtctgaag cctttcagac
     1021 ggcatttgcg gcatttggac gtttaaaact tcatttccaa gctaaatgtg tagtttcggc
     1081 cgggggcggc atatcggttg taaacgccga catttttgtg ttggttgact gcgccgccgg
     1141 cagtttgccg cacattttcc caagtaacat agcggtagtt gaggaggttg tacacgcccg
     1201 cacggagggt aaagtgtttt ttaatcgtgt aataaccgga cacatccaca atataccaag
     1261 ggcgggtacg gcgcgcggtg gcttttgtat tgcggctgtt gccgttgagc aaagcccggc
     1321 tgcccaacaa ctctgtgatt tccttggctt tggaataagt cagcataccg ttcacacccc
     1381 atttgccttc cggttggtca tagcccaagc cgacgacata gcgcgagggt tggatggcat
     1441 caaacaggtg tgattgaata tcggtgcggt ctgcgcgttt tttgatgtcg cggacatgga
     1501 cacgattata ggcaaatgta gaataccaac cttcgggcaa tttatcccat acgccgttcc
     1561 aatcgatttt gcccaaaaca ttgatgccgg taatccgcgc gctttgggca ttgaggtaag
     1621 ccgggtcgcc tttggctgtt tctttgccgt tttgaatttg cgcttcataa ccccggacaa
     1681 tcaaatcgcg gtaggcattg ttgaaccaac ttgcctccaa gttgccgaaa tcgcctttaa
     1741 acacgatgcc ggcttctttg ttgaacgatt tttccggatc gattttgacc gccttgcttt
     1801 gaacacccgc ccgccagccg tacatttccg caaacgaggg caggcggaag ccggttgaag
     1861 tgcggtaagt caaatccagc cagtcggcag gtttgaggac gatgcctgcg ttccaggaca
     1921 gggtgcggtg cgtgccggtg gaaacgctgc tgtcgtccga atgcgtgctg cggtagtcgt
     1981 agcgcaaccc cgcgccgaca tccgcccacc tgcccaaacg gacattgtcc cgaactgccg
     2041 cgtaatagct tttgccgttg atgctgcgcg gcgtgcagtc cgtataagta ttattgccca
     2101 agcggcaaat ttgccccgta accacatttc ccccgcctat gctgacccaa tagggtttgt
     2161 cttggctgcc gttggggctg attttttgat tgccgttttg agtgggtatt ttcgacgaat
     2221 aggcgcgttt ggcgttttga taataataat cctgatggcg gaggtcggaa ccaaagcggt
     2281 caaaaccgag attcacgctc aggttgtggc ggattttggc ggtatcgaag gattttttga
     2341 atgccgcctg caagagcctg tggctttccc cgtaaatcac gcggtcggat ttgtagtagg
     2401 aaaacggctt gtcggcactc ggacggcaat atttgtccga accgtcggca gaacagtgcg
     2461 tctgctgaaa atgattgtcc aaaccgatgc cctgccggtc gtaagagagg cgggcataat
     2521 ccgcccaagt gtctttatcg gcattggtat agacatattc caaaccgtag cggcttttgg
     2581 tgtgcgtctc gtcgtaaaac acgcccgtac cgtattccgc gcccaccagc gcaccgtttt
     2641 cgccgttggt aaacagtccg ccgtatttgt ggttgcccgc gtatttgccg ttgccggaca
     2701 aagaacccgc ctgtttttta tttgcatcaa aaaccgcctt ggtcaggaat gccggaaccg
     2761 tcatatcgcg cgtgtcgaaa gtttgttgcg tgtgttcgag tatgccgccg atgtagtgcc
     2821 gtttgttttc aaaacgaaaa cccgggcgga acagccacga tcggctttcg tatgaaagcg
     2881 gatcggcgag aaagcggttg ggacccgtgt agtctcgggt ggaaaccgtt tgacgttcgt
     2941 ctttgccgac aatatctttt ttcggtttgc ttttacacgt ttcataactc ccgcttttgc
     3001 attcttcttc gaccacaaaa tagacgtagt cgctgctgcc gtcttcaacc ggcaccagcc
     3061 tgttaaagct ctgaacgccg cgtcctgcat cttcgtgggc gcggatttcc cccgcgcgcc
     3121 gacccgtgtg gatcagcaaa gcctccgcac cgccgatgcg ccccgccagc gcgatggatt
     3181 gggtaagccc ccggtttttg ccggaatagg cggttttact ctgaatcccc cactgcctgc
     3241 cttccccgat aacatcgtcg gcggttttgg tttgaaatgc gaccgagccc gccaatgcgc
     3301 cgctgccttg ttcgaccgag tttgagcctt tgctgatttc gacagctttg acgttttcat
     3361 actcgatttc attgattgcg ccgctgctgc ccgccgtcct cgtcccgccc aatgccgcct
     3421 gcgcggtgta ggactgtatt tgcgccaagc cgtccaccgt caaggaaacg cggtttttgt
     3481 ccataccgcg tatcgagtag cccgagcttg cgccgcgccc ctgttcgaca acggcgatgc
     3541 cggggtcgta acgcgtcagg tcgcggatat cgagtacctg ttccttgctg agggtgtcgg
     3601 cggttttgac caatttgccc aaaccggtta cttcgttatc gcggcgggtt ttctgttttt
     3661 tggcttttac ctgtatggta tccaactgtt tttcctgtgc ttgtccggct tgcacatttt
     3721 ctgcataaac gggcagcgcg gtcattaaag acaggcataa aatatttaat cggaacaaat
     3781 gttgctgttg catagtgttt ccctaatctt cgctttcaga cggcatcgga aggagcggtg
     3841 ccgtctgaag ccttattctt gattgttcgg caaccgtgct tattgcacag gctgttggcg
     3901 tttcgcaccg aataccacgg tcgcgctgct tgctgaattt ccatcgccgg atgcaaccgt
     3961 tgccttttcc gtttgtttat cgcccggata ggcaaaccat ccgcccaact cttcggcttt
     4021 aggcccgtaa aaaccgccct gcaccttggc atctgtgata tatgccttag gtgtgccggt
     4081 ggtattgctt tgatcgagat caaaacctaa gtcagcagtt ttcgccgtac cggaaaaacc
     4141 gttgtcctga atcgtggcgg taatggtaaa cgtgggagac gtacgctccc tagccgtcag
     4201 tgttccgttg agttttttct cggcaaaatt cacagtaaat tccgccttgt tgccgccgtt
     4261 ttcatttgaa gcattgccgc tccagcttgt gccgttggca atatgcccgt accaagaccc
     4321 ccgataaacg acgttttggt cgtttggaat ctctttttca tcggtgcgct cgccttgtaa
     4381 gaacatactc tgcgcgtccg tttgggcggc ggcttggctg ccgttgccgc ttcccaccgt
     4441 gttgccggcg gttttgcgtg tcagcaaccc gtatttcaga taattgaggt tggaacagca
     4501 gacttcgact gcataggttt ttgttttgcc attggcatca cctgccgtat ttgaagcggc
     4561 ttgattgcca ttcgccgccg tacctgcttg ggtgtctttt tcatcgcttt tcggcgtgtg
     4621 gtcaaatttg cgggtaaagt ctgttccgcc gtttttacct ttatctgcct gattgctccc
     4681 gatttcggaa tccttgggca ggagcggaat cataatgccg tcgacaacca gttgggcggc
     4741 gttgctgaag ttgtcgagat tttcgacttt cttaccgtcc gatttcagct cgaccgcatc
     4801 caaaaccgtg gtcagcctgg tttcagacgg catagttgcc gcacctgcgc cgcttgaagc
     4861 cgccggagta ttgccatttg cgtttttgtc tttggttttc gcgctgccga caaccgcaac
     4921 ttttttatcg tcgctcaaaa agcggaaacc caattcctca cccttcgggc cgaaaaagct
     4981 gccgcttaaa gaagacgagt cggaaacaaa gggatgttgt ttggtttcat tctcttttgg
     5041 tttgtcggtt gccgttgcct tgccgttgaa gcggtttccc ttaagcgtcg cctcaaggct
     5101 gtaatattgc gtggtgtgtt tatcgttatt agtagtgatt acactgttgt ttttaattaa
     5161 ttttccgctg agggtttttt tctcaaaatc aaccgttatt tccgttgtta atcccaaacc
     5221 tacgtgcccg tcttttttat cattctcttg tttgactata taatccaact ccccggaaaa
     5281 agcactatat cggtcgccgg gtttcgtaga agcattccct aaatctgtaa atttctgatt
     5341 tgctttcaca tcagttaaaa aatcccaagt acctttgtac ataacctccc ctgaaacagg
     5401 aagttttctc gagggatctt tgcctttata aaaaatataa ccgtcaggcc cacttcgggc
     5461 tgtcatcttt ccaccagttg tttcaaattt agtttgaata tgtttataaa aaaaacccga
     5521 ccatacgtat tggaaattgt gatggtccgt aaccttagat atactctgac cttcttgact
     5581 tgaatcttgc aataatgctc cctcacttcc aagtaaggca ttaataatat ttttttgttt
     5641 ggaaggggtt ttgatattac cattatccgt ttgttcccaa tcattttcag agagttctat
     5701 cttatcttct ttaggattca ccctcggatg ccaattccgc cgcttgaagc gcattgcaaa
     5761 accgtatccg ccttggtctt tttgggcttt cggtttttcg gaagaaacat cttgatactt
     5821 tggcgcggga cgcggggctt cggtatcgac agaatcaaga tcgaaactgc cgcctccgcc
     5881 caaacaagca ctcaacaaaa acacaggcag caccatagca gcctgattca ccaatggatt
     5941 gttcataata aatccaattc aattaaagaa tgataaggat tattatttta tttattttta
     6001 acaaatttgc aaatactttt ttattttttt agggaataca ccaaaatccc gtcatttccg
     6061 cgcagtcgtg aatccgaacg cgtccgcacg gaaacctata tcccgtcatt cccacgtata
     6121 tc
//
LOCUS       DAXHRI010000121         6031 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXHRI010000121 DAXHRI010000000
VERSION     DAXHRI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.121"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            160..1503
                     /locus_tag="ACFM75_001639"
     CDS             160..1503
                     /locus_tag="ACFM75_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC6256152.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTGRVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1506..2738
                     /locus_tag="ACFM75_001640"
     CDS             1506..2738
                     /locus_tag="ACFM75_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC6256153.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVSGKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2731..3507
                     /locus_tag="ACFM75_001641"
     CDS             2731..3507
                     /locus_tag="ACFM75_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC6256154.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSVGAQ"
     gene            3507..4133
                     /locus_tag="ACFM75_001642"
     CDS             3507..4133
                     /locus_tag="ACFM75_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC6256155.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4137..4730
                     /gene="nqrE"
                     /locus_tag="ACFM75_001643"
     CDS             4137..4730
                     /gene="nqrE"
                     /locus_tag="ACFM75_001643"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC6256156.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYGFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4744..5961
                     /gene="nqrF"
                     /locus_tag="ACFM75_001644"
     CDS             4744..5961
                     /gene="nqrF"
                     /locus_tag="ACFM75_001644"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC6256157.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHINKREARE
                     GCRLSCQVNVKTDMEIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEKYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDRLAAEFPNFTWHV
                     ALSDPLPKDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
BASE COUNT         1406 a         1638 c         1582 g         1405 t
ORIGIN      
        1 gcttccgcaa ggaaaaaagc agcaaatctg acaaacaggt acaattcctg atagaatcta
       61 acactttgct acattaattt attcaatgct tttgatgcca cgcgacacgg tatcaaaaca
      121 cgccgttttt accttaacca tcacaagtaa tgcaaatata tgattaaaat caaaaaaggt
      181 ctaaacctgc ccatcgcggg cagaccggag caagtcattt atgacgggcc cgtcattacc
      241 gaagtcgcgt tgcttggcga agaatatgcc ggtatgcgcc cctcgatgaa agtcaaggaa
      301 ggcgatgccg tcaaaaaagg ccaagtgctg tttgaagaca aaaagaatcc gggcgtggtg
      361 tttactgcgc cggcttcagg caaaatcgcc gcgattcacc gtggcgaaaa gcgcgtactt
      421 cagtcagtcg tgattgccgt tgaaggcaac gacgaaatcg agtttgaacg ctacgcacct
      481 gaagcgctgg caaacttaag cggcgaagaa gtgcgccgca acctgatcca atccggtttg
      541 tggactgcgc tgcgcacccg tccgttcagc aaaattcctg ccgtcgatgc cgagccgttc
      601 gccatcttcg tcaatgcgat ggacaccaat ccgctggctg ccgaccctac ggtcattatc
      661 aaagaagccg ccgaggattt caaacgcggc ctgttggtat tgagccgttt gaccgaacgc
      721 aaaatccatg tttgtaaggc agctggcgca gacgtgccgt ctgaaaatgc tgccaacatc
      781 gaaacacatg aattcggcgg cccgcatccg gccggtttga gtggcacgca cattcatttc
      841 attgagccgg tcggtgcgaa caaaaccgtt tggaccatca attatcaaga cgtgattgcc
      901 atcggacgtt tgtttgcaac aggccgtctg aacaccgggc gcgtgattgc tttgggtggt
      961 tctcaagtca acaaaccacg cctcttgcgt accgttttgg gtgcgaaagt atcgcaaatt
     1021 actgcgggcg aattggttga cgcagacaac cgcgtgattt ccggttcggt attgaacggc
     1081 gcgattgcac aaggcgcgca cgattatttg ggacgctacc acaatcagat ttccgttatc
     1141 gaagaaggcc gcagcaaaga gctgttcggc tgggttgcgc cgcagccgga caaatactcc
     1201 atcacgcgta caaccctcgg ccatttcctg aaaaacaaac tcttcaagtt caacacagcc
     1261 gtcaacggcg gcgaccgcgc catggtgccg attggtactt acgagcgcgt gatgcccttg
     1321 gatatcctgc ccaccctgct tttgcgcgat ttaatcgtcg gcgataccga cagcgcgcag
     1381 gcattgggtt gcttggaatt ggacgaagaa gacctcgctt tgtgcagctt cgtctgcccg
     1441 ggcaaatacg aatacggccc gctgttgcgc aaagtgctgg aaaccattga gaaggaaggc
     1501 tgattatggg cttgaaacat tttctggaaa aaatcgaacc gcacttcctg ccgggcggca
     1561 aacatgaaaa atggtatgcc ctctacgaag cggcggcgac gattttctac acatccggtg
     1621 cggtaacgcg caaagcggca cacgtccgcg acgcgctcga ctccaagcgc atgatgattt
     1681 tggtgtggct ggctttgttc cccgccatgt tttacggcat gtacaacgtt ggcgcacagg
     1741 cattcggtgc cttaacgccc gatttgctgc aacaaagcat cgccaacgac tggcattaca
     1801 cccttgccga cgctttgggc atcaatatgt cgtctgaagc gggtgtgtcg ggcaaaatgc
     1861 tgttcggcgc gatttacttt ctgccgattt acgcgaccgt atttgtcgtg ggcggttttt
     1921 gggaagtctt gttcgccacc gtgcgcaaac acgaaatcaa cgaaggtttc ttcgtgactt
     1981 cgattctgtt tgccttaatc gttccgccca cgctgccgct gtggcaggct gccttgggta
     2041 ttaccttcgg cgtggtggtt gcaaaagaag tattcggcgg tacgggtaaa aactttatga
     2101 accctgcgct ggcaggccgc gccttcctgt tcttcgccta cccagccaac ttgagcggcg
     2161 atgcggtttg gacggcggtt gacggctatt ccggtgcaac cgcgctggcg caatgggcgg
     2221 cacacggtgc agacggcctg aaaaacgccg taaccggtca aaccatcact tggatggacg
     2281 cgtttatcgg caaactgccc ggctccatcg gcgaagtctc cactttggcg ctcttaatcg
     2341 gcggcgcgtt tatcgtgttt gcccgcatcg cttcttggcg cattattgcc ggcgtgatga
     2401 tcggtatgat tgccatgtct tcgctgttca acttcatcgg ttcggacacc aacgctatgt
     2461 ttgctatgcc ttggtactgg catctggtcg tcggcggctt cgccatcggt atgctgttta
     2521 tggcgaccga ccctgtttcc gcctccttta ccaatgtcgg caaatggtgg tacggcgcgc
     2581 tgatcggcgt gatgtgcgta ttgatccgtg tggtcaaccc cgcttacccc gaaggcatga
     2641 tgttggcgat tctgtttgcc aacctgtttg ccccgatttt cgactatttc gtcgcacaag
     2701 cgaacatcaa acgcagaaag gcgcgcagca atggctaaga aattcgataa agacagcttc
     2761 agcggcacgc tgattgtcgt gttggcggtc agcctgattt gctcggtcat cgttgccggt
     2821 gcggtcgtcg gcttgaaacc catccaagaa aaacaaaaac tccaagacaa acaaggctat
     2881 attttgagcg tggccggttt gatggataag gacaccgaca tcggcaaaac ctttgccgag
     2941 cgtatcgagc aacgcgtggt cgatttggcg accggcgaat atgtgaaaga cgcgccaaaa
     3001 gacttcagcg cgcgcatcgc aggcaaagac cccgcgcaaa gcatccgaat caaacccgaa
     3061 gacgatttgg caggcatcaa aagccgtgcc aaatacactg aggtttattt ggtaaaaggc
     3121 gaagacggca aaatcggcca aatcatcctg ccgatgcacg gtaacggttt gtggtcggtc
     3181 atgtacggct ttgtcgccat tcaaccggac ggcaacacca tcaacggcat tacctactac
     3241 gaacaaggcg aaaccccggg cttgggcggc gaaatcggca atccgttgtg gcagcaaaaa
     3301 ttcgtcggca aaaaactgtt tgacggacaa ggcaaactcg ccctgcacgt cggcaaaggc
     3361 gcgggttcgg acaaagaaca cggcgtagat gccctctccg gcgcgtcgct gacttccaaa
     3421 ggcgtgcaag gttcgttcgc ctactggttt ggcgaaaacg gctatatccc ctacctgaac
     3481 aaattgaaat cagtaggagc acaataatgg ctgatatgaa acgcttgaaa cacttgatgt
     3541 tttcaccctt tatcgacaac aacccgattg ccttgcaggt tttgggtatt tgttcggctt
     3601 tggcggttac caccaaactt cagacggcca tcgtgatggg tatttccgta gctttggtaa
     3661 ccggtttttc cagcttcttc atttcgctgg tacgcaacta catccccaac agcatccgta
     3721 ttatcgtgca aatggcgatt atcgcgtcgc tggttacgct ggtcgaccaa ctcttacagg
     3781 cctttgccta cgaattgtcc aaacagcttt ccgtattcgt cggtctgatt attaccaact
     3841 gtatcgtgat gggccgcgcc gaagcatttg cgatgaaaga gccgccgctg gaaagcctga
     3901 tcgacggcat cggcaacggc gcgggctacg ggatgatgct gcttgtcgtc gccaccgtcc
     3961 gcgaactgat tggctcgggc aaactcttgg gctacaccgt tttccaaacc gtgcaggacg
     4021 gcggctggta tcagaccaac ggcttgttcc tgctcgcccc cagcgcgttc ttcatcatcg
     4081 gctttttgat ttggggactg cgtacctgga aacccgaaca ggcggaggaa taagctatgg
     4141 aacactattt gagcctcttc atcaaatccg tcttcattga aaatatggcg ctatccttct
     4201 ttttgggtat gtgcacgttt ttggcggtat ccaaaaaagt atccaccgca ttcggtttgg
     4261 gtgtggcggt cattttcgta ctcgggctgt ccgtccctgc caaccaactc gtttactcgc
     4321 tgctcaaaga cggcgcgatt gtcgaaggcg tggatttgac ctttttgaaa ttcattacct
     4381 tcatcggcgt gattgccgct ttggtgcaga ttttggaaat gttcttggac aaattcgtcc
     4441 ccgccctcta taacgcgctg ggcatctacc tgccgctgat taccgtgaac tgcgcgattt
     4501 tcggtgccgt atcgtttatg gcgcagcgcg aatacggctt cggcgaatcc gtcgtgtacg
     4561 gcttcggcgc gggcttgggc tggatgttgg cgattgtcgc tttggcaggc attaccgaaa
     4621 aaatgaaata ttcggacgct cccaaaggcc tcaaaggctt gggcattacc tttatcgccg
     4681 ccggcctaat ggcgatggcg tttatgtcgt tctccggcat ccagttataa gaaagggacc
     4741 ggtatggaga ttattttagg tatcgtgatg tttaccgtca tcgttttggc tttggcactg
     4801 atgattctgt ttgccaaatc caagctggtc agcgaaggcg acatcaccat caaagtcaac
     4861 ggcgaaaaag agctgacgat gcccgccggc ggcaaactct tgggcgcgct tgccaacgaa
     4921 ggcatcttta tcccctccgc ctgcggcggg ggcggttcgt gcggtcagtg ccgcgtcgtc
     4981 gtaaaaagcg gcggcggcga catcctgccg accgagctgt cccacatcaa caaacgcgaa
     5041 gcgcgcgaag gctgccggct gtcgtgccaa gtcaacgtca aaaccgatat ggagattgaa
     5101 gtccccgaag aagtgttcgg tgtgaaaaaa tgggaatgca ccgtcatctc caacgacaac
     5161 aaggccacgt tcattaaaga actcaagctt gccattcccg aaggcgaaga agtgcccttc
     5221 cgcgccggcg gctacatcca aatcgaagcc ccgccgcaca ccgttgccta caaagacttc
     5281 gacattcccg agaaatacca cgaagactgg gacaaataca atctgtggca atacgtttcc
     5341 aaagtggacg aaccgatttt gcgcgcctac tctatggctt cgtatcctga ggaaaaaggc
     5401 atcattatgc tgaacgtgcg tatcgccacg ccgcctccgc gcgtacccga tgcgcctccg
     5461 ggacaaatgt cgtcctacat ctggtcgctc aagcccggcg acaaagttac gatttccggc
     5521 ccgttcggcg aattcttcgc caaagacacc gatgccgaaa tggtattcat cggcggcggc
     5581 gcaggtatgg cgccgatgcg ttcccacatt ttcgaccagt tgaaacgttt gaactccaaa
     5641 cgcaaaatca ccttctggta cggcgcgcgt tccaaacgcg agatgttcta tgtcgaagac
     5701 ttcgaccgac tcgcggcaga gtttccgaac ttcacgtggc acgtcgccct gtccgacccg
     5761 ttgcccaaag acaactggga cggctacaca ggcttcatcc acaacgtggt ttacgaaaac
     5821 cacctgaaaa accacgaagc accggaagac tgcgaatttt atatgtgcgg ccctccgatt
     5881 atgaaccagt ccgtcatcaa aatgctcaaa gacttgggcg tggaagacga aaacatcctc
     5941 ttggacgatt tcggcggtta aaacccaacc tcaaatacaa aaggaagaaa cctcggtttc
     6001 ttcctttttc tatccgatac cgtccgaaca c
//
LOCUS       DAXHRI010000122         6014 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXHRI010000122 DAXHRI010000000
VERSION     DAXHRI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.122"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..208
                     /locus_tag="ACFM75_001645"
     CDS             <1..208
                     /locus_tag="ACFM75_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256158.1"
                     /translation="FSELYMFQQRMAHQAPHCVLPERDYPPIRFYRYKQMGFNRKGMG
                     IKSISDISRAMPSENQSPLSDGIV"
     gene            285..1232
                     /locus_tag="ACFM75_001646"
     CDS             285..1232
                     /locus_tag="ACFM75_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6256159.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="ACFM75_001647"
     CDS             complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="ACFM75_001647"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC6256160.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPNDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2508..3311
                     /locus_tag="ACFM75_001648"
     CDS             2508..3311
                     /locus_tag="ACFM75_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC6256161.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEISVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3369..5588)
                     /locus_tag="ACFM75_001649"
     CDS             complement(3369..5588)
                     /locus_tag="ACFM75_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC6256162.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1479 a         1880 c         1446 g         1209 t
ORIGIN      
        1 tttctccgag ctttatatgt ttcaacagag gatggcacat caagcaccgc actgcgtgtt
       61 gcccgaacga gactaccctc cgattagatt ctatcgctat aaacagatgg gtttcaaccg
      121 aaaaggaatg gggataaagt ccatttccga catctctcgg gcgatgccgt ctgaaaacca
      181 atctccactt tcagacggca ttgtttaggc gcggtcattt tgcaaattaa ggttaaggcg
      241 tgtatgcttg ccttccctta aaaacaaaca cgcaacgcat tattatgaat atcctcagta
      301 aaatcagcag ctttatcgga aaaacatttt ccctctgggc cgcgctcttt gccgccgccg
      361 cttttttcgc gcccgacacc ttcaaatggg cggggcctta tattccttgg ctgttgggca
      421 ttattatgtt cggtatgggt ttgacgctca aaccttccga cttcgatatt ttgttcaaac
      481 atcccaaagc cgtcatcatc ggcgtaatcg cacaattcgc cattatgccg gcaaccgcct
      541 ggctgctgtc caaactgttg aacctgcctg ccgaaatcgc ggtcggcgtg attttggtcg
      601 gctgctgccc gggcggtacg gcttccaatg tgatgaccta tctggcgcgt ggcaatgtgg
      661 ctttgtcggt tgccgttacg tctgtttcca ccctgatttc cccattgctg actcccgcca
      721 tcttcctgat gcttgccggt gaaatgctgg aaatccaagc ggccggtatg ttgatgtcca
      781 tcgtcaaaat ggttttgctc cccattgttt tgggtttgat tgtccataag gttttgggca
      841 gtaaaaccga aaagctgacc gatgcgctgc cgctggtttc cgttgccgcc atcgtgctga
      901 ttatcggcgc ggttgttggg gcaagcaaag gcaagattat ggaaagcggt ctgctgattt
      961 ttgcggttgt cgtactccac aacggcatcg gctacctgct cggcttcttt gccgccaaat
     1021 ggaccggcct gccttatgat gcacaaaaaa cgctggccat cgaagtcggt atgcaaaact
     1081 cgggtctggc cgccgcgctg gccgccgcac actttgccgc tgcgccggtc gttgccgttc
     1141 cgggcgcatt gttcagcgtg tggcacaata tctccggctc gctgctggca acttattggg
     1201 cggccaaagc cggtaaacat aaaaaaccct aaacgccttt catcataatg ccgtctgaac
     1261 ggggcaaacg aaaccggcgg tttccgcccg accccttcag acggcctttg tttgaaatcg
     1321 gcgggaacga ttgcccggct gctccggttc ggctgcctgc gttcgccccg cctttcaagc
     1381 cgcctcactc ccttacataa accacttcga catcgtagtc gtcttcgttc caatcgtcat
     1441 cgtcgtccgc gccgaatttg tcctgccatt cctcttcatt gctcaaagac gaatccaaac
     1501 cggcttcaag acggaggaca gacaataaat gatatatgtc gtttggaatc ggtgcttcaa
     1561 acgaaacggt ttctccggtt ttcggatgag tgaaacttaa gcggtaggcg tgcaatgcct
     1621 gacgcgcacc caaactttta acggcttctt tcaccgtgtc gccgcacgga tggcgcgggt
     1681 tgccgtaaac cgggtcgccg gcaagcggat ggttggcctc acgcatatgg acgcggattt
     1741 ggtgcgtcct gcctgtttcg agcgagcatt cgatatagct gtgggcaaga tagcgttcca
     1801 acactttgac gtgggtaacg gctggtttgc cgccgaattt gacgactgcc attttcaggc
     1861 ggttgtgcgg atcgcgtccg atttgggttt cgattttacc gtcaaaggga acgatgccgt
     1921 tggcgacggc gcggtaaatg cgtttgaccg tgcgctcttg aagctgcctc acgagggaat
     1981 tttgcgccga cagggttttg gcaaccacca tcagcccgct ggtttctttg tccaaacggt
     2041 gtacgatgcc cgcgcgcgga atttgggaca gctcggggca atgcgccaac aggccgttga
     2101 gcagcgtccc cgtccagttg cccgccgccg gatgcaccac cagtccggcc ggtttgttga
     2161 cgacgatgac ggtatcgtct tcgtaaacga tatccaaagc cataggctct ggaacaaacg
     2221 ccagattttc ctcactcgga cggacggtta cacaaatttg ctcgccgcct atcattttgt
     2281 ctttgggttg cgaaggttta tcgtttacaa taaccgcgcc ttctttaatc catgatgtca
     2341 ggcggctgcg cgagtagtcg ggcagaagtt tcgccaacac cgcatccaac cgcccgccgg
     2401 caagctcgag cggaacggtc agaccgatac ggttttcagt ttcggaagct gacgcaaagt
     2461 ctgaatcgtc gctataatcg gattcattat caaaggaagt attctgcatg aaaaaaattc
     2521 ttttaacggt ttcattaggt ttggcactga gtgcctgtgc cactcaaggt acggtcgata
     2581 aagatgctca gattacccaa gattggagtg tggagaagct ctatgccgaa gcccaggacg
     2641 agctgaacag cagcaattat acgcgggctg tcaagttata cgaaatcttg gaatcgcgct
     2701 tccccaccag ccgccatgcc cggcagtcgc aactggatac cgcatacgcc tattataaag
     2761 acgatgaaaa agacaaagct ctggcggcaa tcgaacgctt ccgccgcctt cacccgcaac
     2821 acccgaatat ggattacgcg ctgtatctgc gcggcttggt gctgttcaac gaagaccagt
     2881 ccttcttgaa caaactggcc tcgcaagact ggtccgaccg cgacccgaaa gccaaccgcg
     2941 aagcgtacca ggcgtttgcg gaactcgtcc aacgcttccc gaacagcaaa tacgccgccg
     3001 atgcaaccgc acgcatggtc aaactggtcg atgcactggg cggcaatgaa atatcggtgg
     3061 cgcgctacta catgaaacgc ggtgcataca tcgccgccgc caaccgcgcc caaaaaatca
     3121 tcggcagcta ccagaatacg cgttatgtcg aagaatcgct cgccatattg gaacttgcct
     3181 accaaaaact cggcaaacca cagcttgccg ccgatacgcg ccgcgtgttg gaaaccaact
     3241 tcccgaaaag cccgtttttg acgcacgctt ggcagcccga cgatatgccc tggtggcgtt
     3301 actggcatta agcccatctt cgtaatgccg tctgaagccg tttcagacgg cattttatgt
     3361 ttcaaacctc attcaaacgg ttttttctgc caatagaatt tataccctct caaacgttga
     3421 gccttcacgc cgccgcgtcc caagccgaat tgcagtgcgc ccgacaaatc ggtacgcagc
     3481 aatttgatgc cgtgtgcgcg gacacggttc tgcaccgctt cggtcggatg tttgtaggcg
     3541 ttggcataac cgcttgaagc aacggcatat tcgggcgaga cggcattgag gaacacgccc
     3601 gacgaggacg tattgctgcc gtgatgtccc aaaaccaaaa cctggctgta caggttgcct
     3661 ccatacttgc cgaccaggct ttcctcgccc ttcgtatcca aatcgcccgt taccagcagt
     3721 gccgcaccgc ccgccacaac acgcaaaaca caacttttcc cattatcatc gatgtttttg
     3781 cgttcagacg gcatcaaaaa ctcaaaatcc acgccgtccc attgccaacg ctgttccgca
     3841 caatgccgcg ccccctcata aaattccggc tgtccggcat aaatcccgcc gttcggtatc
     3901 ttgccgactg cctgaaaacc gccgtcgtgg tcgctgtcgt gatgcgacag aaccagcttg
     3961 tccaaacggc ggacacccgc cgcattcaaa ctcggcacaa tccccatctg cgccgccgct
     4021 gccgttccgg tgtcaaacaa aagatgatga tttgccgtct gaaccgacac cgacaaaccc
     4081 tgccccgcat cccaaaccgt aaccgcaacc tcattttccg gcacgccggg tgaacggtaa
     4141 aacacaaacc ctgccaacag caacaccgcc cacggacgca aacccaagcc gcgcggcagc
     4201 agcaacagca aagcggcaca caccgccaac accaacagcg gcaaaggtgc ggaggcaacg
     4261 gcaaactcgg gcgacacatc ggcaagccac accaaaaacc gcaaagtata ttccgccaaa
     4321 aatgccccca actgttgcaa aggcgcaaac ggcacgaccg aacccagcaa cgccagcggc
     4381 gtcaataccc aagaaaacca cggaattgcc accgcattga ccaaagggct gaccaaaggc
     4441 agcgaagcaa acagataacc gagcaaaacc agcgacaaca ccgaagccgc ccactgcccg
     4501 cgcaaagcag tttgccgttt tccctcgtgc aaacgccccg agcacgccca tatcagggcc
     4561 gccaccaaac cgaaagacag ccaagtcccc acacccaaga ccgccgacgg gtcgaacagc
     4621 agcaccgccg ccaacgcctg ccaccacgtc gcccacgccg acaatcttcc cctgcgccaa
     4681 gcccacgcaa acgccgccaa catcaaaacg ctgcgctgcg tcggcacgga aaaaccggca
     4741 agcagcgcgt aaaacagcgc gcctgcacac cccgccgcca aaacccacgc gcgcggccgg
     4801 gcaggaaggc gcggggaaca ggcaagcagc cgcttcgcca gccacgcaaa cattaccgcc
     4861 accatcgtaa cgtgcaaacc cgaaatactg accaaatgcg tcagccccaa cggccggaac
     4921 gcctgccaca attcggggcg caatgccgac tgttcgccca cgctcaacgc gcgcatcagc
     4981 ccgatgccgt ctgaaagccc gccgtccgca tccgcctgct gccaattacg gctgatgcgg
     5041 ctgcgccaaa ccgcaatccc ccaaccgctt ccgccatgca gcaaaaccct gtccgcaccg
     5101 accgtccccg cgccgcctat tccgttggat aatgcccacg cctcacggtt caaaccgcgc
     5161 aggttcaact ccccgacgac agggtgcaca cgtgccgtta tccgccatct gctcccgacc
     5221 gcccattcgc gccgtttgta gtccgacagc agcaaatcaa acgtccgacc accgctgtcc
     5281 acagcctttg ccgcaaactg cacacgccgc ccgtccgacc tcggcatatc cgccacttcc
     5341 accgtcaaag gcacgcctga aacggcttct gcccaccatt gggaagacaa tgccgcctcc
     5401 gtcctgaata cgccgtaagc cgcgcccgcc aacacgcaca gcatcagacc ggcaaacgca
     5461 aaacgccttg caaacacagc aaaaaccgca aaagccgcca gccaaaacag ccaatgcggc
     5521 acgaccggca gcgcgaacga agccgccgca ccgacaaccc aacacggcaa aaaccaacga
     5581 cggaacacgt ctccccaccc cagatatttc aaaaataaac ggataataaa gcaaatccga
     5641 aaaaccgtca taacaagatt tgaaaatgat atagtggttt aaatttaaat caggacaagg
     5701 cgacgaagcc gcagacagta caagcagtac gacaaggcga ggcaacgctg tactggtttt
     5761 tgttaaacca ctataagaat gcaaacgaaa agcctcagta ttatccgaga caacggcaaa
     5821 ctttccatcg tcattcccac gaacccacat cccgttatta ccacgaaagc gggaatctag
     5881 gactcagggt taagaaaacc tacatcccgt cattccctca aaaacagaaa accaaaatca
     5941 gaaacctaaa atcccgtcat tcccacgaaa gtgggaatct aggtctgtcg gtgcggaaac
     6001 ttatcgggta aaac
//
LOCUS       DAXHRI010000123         6010 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXHRI010000123 DAXHRI010000000
VERSION     DAXHRI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.123"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            43..456
                     /locus_tag="ACFM75_001650"
     CDS             43..456
                     /locus_tag="ACFM75_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC6256163.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            502..1131
                     /gene="nth"
                     /locus_tag="ACFM75_001651"
     CDS             502..1131
                     /gene="nth"
                     /locus_tag="ACFM75_001651"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC6256164.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1269..2768
                     /locus_tag="ACFM75_001652"
     CDS             1269..2768
                     /locus_tag="ACFM75_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC6256165.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGKEITVAVKLGN
                     ASEQTGSSSEPDKAPYAEHQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            2970..4490
                     /locus_tag="ACFM75_001653"
     CDS             2970..4490
                     /locus_tag="ACFM75_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC6256166.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRTKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDGSWGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISMVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVDPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKLANA"
     gene            4547..5632
                     /gene="nagZ"
                     /locus_tag="ACFM75_001654"
     CDS             4547..5632
                     /gene="nagZ"
                     /locus_tag="ACFM75_001654"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC6256167.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGLKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5856..5932
                     /locus_tag="ACFM75_001655"
     tRNA            5856..5932
                     /locus_tag="ACFM75_001655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5890..5892,aa:Pro,seq:cgg)
BASE COUNT         1380 a         1822 c         1505 g         1303 t
ORIGIN      
        1 tacaatccgt cgccgatttt tgcctgtttg agactgtttg caatgatgcc cgcctacgcc
       61 tacgccgcgc tcgctttttc cgccttcact tccgccaccc tcctgcccgg cacatccgaa
      121 gccgcctttg ccctgttcgt ccaccgcttt cccgaacacg catacggcgc gttgctgtgc
      181 gccggccttg ccaacggatt gggcagtatg gtttcctatt ggatggggcg tttgctgccc
      241 tcccgaaaaa tgccgtctga aaaaacactg aatctgatgc ggcgtttcgg tatttggctg
      301 cttgcgtttg cctggctgcc cgtcgtcggc gacgcactgc cgcttgccgc cggctggctg
      361 cggttgaatc cgtggacaag cgggctgatg ctggttatcg gcaaaacggc gcgttacgcc
      421 ttcattctgt ggggaatgca atattacgcc gcctgaacca tacgttataa tgccgaacac
      481 acaaacccac cgtataaaaa aatgaacaga cacatccgcc aagaaatctt cgaacgcttc
      541 cgcgccgcca acccccatcc gaccacagaa ctcaacttca actccccttt cgagctttta
      601 atcgccgttt tactgtcggc acaggcaacc gacgtcggcg taaacaaagc gacggcgaag
      661 ctgtttccgg ttgccgatac gccgcaggcg atgctggatt tgggtttgga cggcgtgatg
      721 gaatatacga aaaccatcgg gttgtataaa accaagtcca agcacatcat gcagacctgc
      781 cgcatcctgc tggaaaaata caatggcgaa gtgccggaag accgcgaggc tttggaatcg
      841 ttgccgggcg tggggcgcaa aacggcaaac gtggtattga acacagcgtt cggacacccc
      901 gttatggcgg tcgatacgca tattttccgc gtgtccaacc gaaccaaaat cgcccccggg
      961 aaagatgtgc gcgaagtcga agacaaactg atgcgcttca ttcctaaaga atttctgatg
     1021 gacgcgcacc actggctgat tttgcacgga cgctacacct gcaaggcact caaaccgcaa
     1081 tgccaaacct gcatcatcaa cgatttgtgc gaatatcccg ccaaagcctg accgccgccg
     1141 cccttcaaaa tgaaacgctt gaaaattcgg acggacgttg tagaatttca acctatgccg
     1201 tctgaagccg acaaaatgcg cttcagacgg cattaaagcc ggtaccggca tccgacaaag
     1261 gacaaaacgt gttcaaaaaa taccaatacc tcgctttggc agcactgtgt gccgcctcgc
     1321 tggcaggctg cgacaaagcc ggcagctttt tcggtgcgga caaaaaagaa gcatccttcg
     1381 tagaacgcat cgaacacacc aaagacgacg gcagcgtcag tatgctgctg cccgactttg
     1441 tccaactggt tcaaagcgaa ggcccggcag tcgtcaatat tcaggcagcc cccgccccgc
     1501 gcacccaaaa cggcagcggc aatgccgaaa ccgattccga cccgcttgcc gacagcgacc
     1561 cgttctacga atttttcaaa cgcctcgtcc cgaatatgcc cgaaatcccc caagaagaag
     1621 cagatgacgg cggattgaac ttcggttcgg gcttcatcat cagcaaagac ggctatattc
     1681 tgaccaatac gcacgtcgtt accggcatgg gcagtatcaa agtcctgctc aacgacaagc
     1741 gcgaatatac cgccaaactc atcggttcgg atgtccaatc cgatgtcgcc cttctgaaaa
     1801 tcgacgcaac ggaagagctg cccgtcgtca aaatcggcaa tcccaaagat ttgaaaccgg
     1861 gcgaatgggt cgccgccatc ggcgcgccct tcggcttcga caacagcgtg accgccggca
     1921 tcgtgtccgc caaaggcaga agcctgccca acgaaagcta cacacccttc atccaaaccg
     1981 acgttgccat caatccgggc aactccggcg gcccgctgtt caacctgaaa ggacaggtcg
     2041 tcggcatcaa ctcgcaaata tacagccgca gcggcggatt catgggcatt tccttcgcca
     2101 tcccgattga cgttgccatg aatgtcgccg aacagctgaa aaacaccggc aaagtccaac
     2161 gcggacaact gggcgtgatt attcaagaag tatcctacgg tttggcacaa tcgttcggtt
     2221 tggacaaagc cggcggcgca ctgattgcca aaatcctgcc cggcagcccc gcagaacgtg
     2281 ccggcctgca ggcgggcgac atcgtcctca gcctcgacgg cggagaaata cgttcttccg
     2341 gcgaccttcc cgttatggtc ggcgccatta cgccgggaaa agaagtcagc ctcggcgtat
     2401 ggcgcaaagg taaggaaatc accgttgccg tcaaactggg caatgcttcc gaacaaaccg
     2461 gttcctcgtc cgagccggac aaagcccctt atgccgaaca ccaatccggt acgttctcgg
     2521 tcgaatccgc aggcattacc cttcagacac ataccgacag cagcggcgga cacctcgtcg
     2581 tcgtacgggt ttccgacgcg gcagaacgcg caggcttgag gcgcggcgac gaaatcctcg
     2641 ccgtcgggca agtccccgtc aatgacgaag ccggtttccg caaagctatg gacaaggcag
     2701 gcaaaaacgt ccccctgctg gtcatgcgcc gtggcaacac gctgtttatc gcattaaacc
     2761 tgcaataaca cacataattt aaccaaaata ttttttaaat gataaaatgc ccgtcaatac
     2821 cgattaacgg gcattttaca aaaatcagtt ggttgatttt aaacaaatat tttttacgac
     2881 aaaaaatatt tccgcccatt ttgccaacaa aacccaaacc ggaggcggac agatacgctc
     2941 ccatccggac gcttaacacc cccataacca tgcagctgat cgactattca cattcatttt
     3001 tctcggttgt gccacccttt ttggcactgg cacttgccgt cattacccgc cgcgtactgc
     3061 tgtctttagg catcggtatt ctggtcggcg ttgccttttt ggtcggcggc aaccccgtcg
     3121 acggtctgac acacctgaaa gacatggtcg tcggcttggc ttggtcagac ggcgattggt
     3181 cgctgggcaa accaaaaatc ttggttttcc tgatactttt gggtattttt acttccctgc
     3241 tgacctactc cggcagcaat caggcgtttg ccgactgggc aaaacggcac attaaaaacc
     3301 ggcgcggcgc gaaaatgctg accgcctgcc tcgtgttcgt aacctttatc gacgactatt
     3361 tccacagtct cgccgtcggt gcgattgccc gccccgttac cgacaagttt aaagtttccc
     3421 gcaccaaact cgcctacatc ctcgactcca ctgccgcgcc tatgtgcgtg ctgatgcccg
     3481 tttcaagctg gggcgcgtcg attatcgcca cgcttgccgg actgctcgtt acctacaaaa
     3541 tcaccgaata cacgccgatg gggacgtttg tcgccatgag cctgatgaac tattacgcac
     3601 tgtttgccct gattatggtg ttcgtcgtcg catggttttc cttcgacatc ggctcgatgg
     3661 cgcgtttcga acaagccgcg ttgaacgaag cccacgatga aactgccgtt tcagacggca
     3721 gctggggcag ggtttacgca ttgattattc ccgttttggc cttaatcgcc tcaacggttt
     3781 ccgccatgat ctacaccggt gcacaggcaa gcgaaacctt cagcattttg ggggcatttg
     3841 aaaataccga cgtgaacact tcgctggtat tcggcggcac ttgcggcgtc cttgccgtcg
     3901 ttctctgcac gctcggcacg attaaaaccg ccgactatcc caaagccgtt tggcagggtg
     3961 cgaaatccat gttcggcgca atcgccattt taatcctcgc ttggctcatc agtatggttg
     4021 tcggcgaaat gcacaccggc gattacctct ccacactggt tgcgggcaac atccatcccg
     4081 gcttcctgcc cgtcatcctc ttcctgctcg ccagcgtgat ggcgtttgcc acaggcacaa
     4141 gctgggggac gttcggcatt atgctgccga ttgccgccgc catggcggtc aaagtcgatc
     4201 ccgcgctgat tatcccgtgt atgtccgcag taatggcggg ggcggtatgc ggcgaccact
     4261 gttcgcccat ctccgacacg accatcctgt cgtccacagg cgcgcgctgc aaccacatcg
     4321 accacgttac ctcgcaactg ccttacgcct taaccgttgc cgccgccgcc gcatcgggct
     4381 acctcgcatt gggtctgaca aaatccgcgc tgttgggctt tggcacgaca ggcattgtat
     4441 tggcggtgct gatttttctg ttgaaagata aaaaactcgc caacgcctga cccctttccc
     4501 tgttcagacg gcatatgccg tctgaagctt tttggagcaa accgaaatga ctgttcccca
     4561 tattccgcgc ggccccgtca tggcagacat tgccgccttc cgcctgaccg aagaggaaaa
     4621 gcaacgcctg ctcgatcccg ccgtaggcgg catcatcctc ttccgccgca acttccaaaa
     4681 catcgagcag ctcaaaacac tcaccgccga aatcaaagcc ctgcgcacac ccgaactcat
     4741 tatcgccgtc gatcacgaag gcggcagggt gcaacgtttc atcgaaggct tcacccgcct
     4801 gcccgcaatg aacgttttag ggcaaatttg ggacaaagac ggcgcgtccg ccgccgaaac
     4861 cgccgccgga caagtcggcc gggtgttggc aaccgagctt tccgcctgcg gcatcgattt
     4921 gtccttcacg ccagtcttag atttggactg gggaaactgc cccgtcatcg gcaaccgcag
     4981 tttccaccac aatcccgaag cggtcgccca cctcgccctc gcccttcaaa aaggtttggc
     5041 aaaaggcggt atgaaatcct gcggcaaaca ttttcccgga cacggatttg tcgaaggaga
     5101 cagccatctg gtcttgccgg aagacaggcg cagcctgtcc gaactcgaag ccgccgacct
     5161 cgcccccttc cacattatga gccgcgaagg tatggcggcg gttatgcccg cccacgtcgt
     5221 ttatccacaa gtggacacaa agcccgcagg gttttccgaa atctggctca aacaaatttt
     5281 gcgccgcgat atcggattca aaggcgtgat tttctcggac gatttgacta tggagggcgc
     5341 gtgcggtgtc ggcggcctca aagaacgcgc ccgcatttcc tttgaggcag gttgcgacat
     5401 cgtcctcgtc tgcaaccgtc ccgaccttgt ggacgaactg cgcgaagatt tccgcattcc
     5461 tgacaatccc gctttggcgc aacgttggca atatatggcc aacacgctcg gcagtgccgc
     5521 cgcgcaagcc gttatacaga cggcggattt ccaagcggcg caagcctttg ttgccggcct
     5581 tgcctcgccg caggacacgg cgggcggcgt gaaagtcgga gaggcctttt aaaccggctg
     5641 cgtttcaaca atctgccgat ttcaaccgtt tgtcagggaa aggcaatgat tttccggtta
     5701 agtagaaatt tatcctattg aattacttga agataaggat gcttatcaac actgtattca
     5761 aacacaacgt tttaaatccg cccgacacgc ggcaaaaagg caaaacggta taggcacgcc
     5821 gtattttttg cgctataatc acgccttact taattcggaa tgtagcgcag cctggtagcg
     5881 cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tcattccgac catattcaaa
     5941 ccgcctcggc atggattgcc ggggcggttt ttccttttga cgtcaacaga aaaatgccgt
     6001 ctgaaacaga
//
LOCUS       DAXHRI010000124         6001 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXHRI010000124 DAXHRI010000000
VERSION     DAXHRI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.124"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(52..360)
                     /locus_tag="ACFM75_001656"
     CDS             complement(52..360)
                     /locus_tag="ACFM75_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256168.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(344..646)
                     /locus_tag="ACFM75_001657"
     CDS             complement(344..646)
                     /locus_tag="ACFM75_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC6256169.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(720..3083)
                     /gene="pheT"
                     /locus_tag="ACFM75_001658"
     CDS             complement(720..3083)
                     /gene="pheT"
                     /locus_tag="ACFM75_001658"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC6256170.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAGTAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSNGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSVKGIAREMSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3119..3637)
                     /locus_tag="ACFM75_001659"
     CDS             complement(3119..3637)
                     /locus_tag="ACFM75_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256171.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3644..4708)
                     /locus_tag="ACFM75_001660"
     CDS             complement(3644..4708)
                     /locus_tag="ACFM75_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6256172.1"
                     /translation="MIKSFELEKFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4885..5877)
                     /gene="pheS"
                     /locus_tag="ACFM75_001661"
     CDS             complement(4885..5877)
                     /gene="pheS"
                     /locus_tag="ACFM75_001661"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC6256173.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
BASE COUNT         1515 a         1542 c         1268 g         1676 t
ORIGIN      
        1 ttttttagcg tgtaaaacaa aaccggctgc atacctgcaa ccggcctcaa atcagcacaa
       61 ttccttatcc aaatccgcca acaggtcttt cagaccgtct ccgatttcct gaagcgtaac
      121 cggacggttg ccgtccgaac ccgattccgc accttctgca tacggtgcaa acgtgctctg
      181 caatttcaac agtttcacca catccaaacg ggtgtagcgt tcgccgccgt aaccgacaac
      241 cacaccgtga ttatgctgcc attgcgcaaa accatagggg ctgatttgca acagtccgca
      301 caactcgtcc agcgtgaaat agcgttttgc gggaaccttc aaatcaacgt tgtttgtcat
      361 agtagtgttc caccatgctt ttgagtttct ggctggcatg gaaagttacc acgcggcggg
      421 cggtaatcgg cacttcctcg ccggttttag gattacgacc cgggcgttgc ggcttgtcgc
      481 gcaactggaa atttccgaaa ccggaaattt tgatttcttc gccgcttgcc aaagtgctgc
      541 ggatttcttc aaaaaagagt tcgacgattt ctttggcatc gtttttggtg acgttgctga
      601 ctttgtctac caaaatatcg gccagttctg ctttagtgag agtcatatga ttaccttctg
      661 ttgtaacagt tgtggattat taccaatttt tatttaaaat tcaagcgaat atttattttt
      721 taactgcgaa gccgcgcccc tgccgccgtt gccgcgccaa tcagttttcc gataagcggc
      781 tcgactgcct catccgtcag cgtgttttcc atatcctgca aaatcacttt gaccgccacg
      841 ctcttcatcc cttcgggcaa acccgtgccg cgatacacgt caaacacgct gatttcctgt
      901 accaacttgt ttgccgcgcc tttcaagaca agcagcaaat catcatggct catagcttcc
      961 ggcatcacaa acgccaaatc gcggcgcacc ggctggaatt tcgatacggc ccgatagcgc
     1021 gttttcccgc attccaacac ggccgccata tcgatttcaa ataccagcgg cgcttgcggc
     1081 aggtcgtatt tttgcagcca tttcggatgc agttcgccga caaagccgat gactttgccg
     1141 tctgaaacga tattggcggc gcgtccggga tgcagggcag gatgtccggt tttaacgaac
     1201 tcgactgctt tgtttttcaa cagattttcc acatctgcct tgatgtcgta aaaatctgca
     1261 ttgcgcgttt tcccgcccca ttgttcgggc attgccgcgc cgtaccacaa gccgccgatg
     1321 cgttcgtttt ggacaaaccg gccgtctgaa cctttgccga acacgcgggc gatttcaaac
     1381 acgcgcacgc ggttttgttt gcggttcagg ttgttttgca aaatttccac caagccgccg
     1441 atgagtgtgg aacgcatcac ggcatattgc gccgccaacg ggttttgcag gcggatgggg
     1501 tcggcgttgg cggcaaaatc ttgttcccac tgctcgtcaa cgaaggcata gctgaccact
     1561 tcgcggtagc cgcgcgccgc catttcgttg taaacggcaa aacgcgggcg gcgtgtttcg
     1621 ggcagttcca gcattttcag acggcctgac gtgtaatcgt cggggatgtt ttcatagccg
     1681 taaacgcgtc cgatttcttc aatcaaatca gcctcaattt cgatgtcaaa acggaagctc
     1741 ggcgcggtaa cgcggaagcc ttccgccgtt ttctcgggct gcaggcccaa gtgttgcaaa
     1801 atggtttcca cctgttcggc aggaatgtcc acgcccaaca cggttttcag acggcccaaa
     1861 cgcaatccga cctgcttcgc ttcgggcaat tcgccttgcg cttccaccat ttcgcctgcc
     1921 gcaccgccgc aaatctgcaa caccaattcg gtagcacgtt caatggcatc cgcctgcaaa
     1981 cggtaatcca cgccgcgctc gaagcggaac gacgaatccg aaccgaaacc gtattggcgc
     2041 gatttgccgg cgatgatttc gggcgcaaac caagccgctt ccagcacgat attttgcgtg
     2101 ccgtctgaaa ccgcgctcgc cgcgccgccc attaagcccg ccaaactcaa cacgcctttt
     2161 tcgtccgcca cgaccagcgt gttttcagac agggaaacgg ttttctcgtt caggcattcc
     2221 agcgtttccc cttcgcgcgc gcggcggatg tgcaggctgc cggaaagttt gtcggcatca
     2281 aaaacgtgca tcggctgacc gatttccagc atcacataat tgccgatgtc caccagcgcg
     2341 gaaatactgc ggatgccgct gcgctccaaa cgttgtttca tccaatcggg agtagccgct
     2401 ttcgcattga cattttcaat aacgcggctg ataaaacggc cgcaatcggc aggcgcgtta
     2461 atctgcacgg gctgttttcg actgcccgtg atcggcgcgg tatggatttc gggctgcctg
     2521 aacgcgcacc ccgtcaatgc ggacatttcg cgcgcaatgc ccttaacact caagcaatca
     2581 gcgcggttag gcgtaatttt caacgtaaac agcgtatcgt ccaaatccaa gtattcacgg
     2641 atattggtac cgacgggcgc gtcttcaggc agaatgtgca ggccgttcac accgtcgtcg
     2701 ggcagaccga gttcgtcggt ggaacacaac atcccgttcg acaccacacc gcgcatcttg
     2761 gtcggcttga ttttgaaatt gcccggcaaa acggcgcccg gcaacgaaca cggcactttg
     2821 atgcccgctt tcacattcgg cgcaccgcac acgatctgaa ccaactctcc cgtacccgca
     2881 tcaactcggg taacgttcaa acggtctgca tccggatgtt tttcaacgga tttcacttcg
     2941 gcaacaacca cgcccgcaaa ggcaggtgcg gcagtcccgg cttcttccac ttccaagccg
     3001 gacatcgtta acaggtgttc cagcttatcg gcggaaagtt cggtatcggc ttgggttttc
     3061 agccatgagt aggagaattg catgttatgt tctcaattaa ctaattaact ttagattttc
     3121 aagagctagc taaacctttt tgaatggcta agtccctatt aattacttct acagaaatgt
     3181 tccataaaga aatcaaccgc aaagtatctt tcttattaaa ttcttgcttt attaattcat
     3241 tcaattgccc aagataatta agcggcacat ccgggataac cagtaataat ttgatttcta
     3301 tgctacccca ttgcaaattt gaaaaagcac taggcataat tgtttctttg atatcttctc
     3361 tgcctaatat tcccaaaatt tcaacattta atgcattaca ccatttttga taaatctctg
     3421 aaatatattc atcaaaacgc tcaggagatc tttcttgatt tggaatactt tgttttgcct
     3481 caacaaataa caaattatct tcttgcagat agataaactc tactgtctta ataccttctt
     3541 gccttctctt ccggatatat ggttctatat aaaacaggtt attttttact agattaaaag
     3601 ttatacctga ttcagtgaga tttattattt cactcataaa aacctaacca atccatgctg
     3661 tttccggaga taaaacacct atttcttctc tgaacaatcg aatagactca ccaataattt
     3721 cattatccgg catgctattt tgtaaatttt cctgcaccca agtagtctgt tccttttctt
     3781 ttccaggcat aaaaacagga atactttcac tatgctttct agctaataaa cacagcttct
     3841 tgataacaaa atatgaatgc gtactcataa aaatctgtat tcccaatttg gatttagata
     3901 aggaaaacag aatttctaga aattgattaa ttgctttcgg atgtagatta gcttctggct
     3961 catcaataaa taagattgat ccaggactca aaaaccgatt tcctaataga gtgtctaata
     4021 tcccaatctt ttttaccccc tctgctgtag aattgataga gaaagtttgt ctatcttttt
     4081 catataccca tgccttcttt ttatcattgt agctaacata accgccaaac aatgcttcta
     4141 gatcctttct agcttccgct gcctcagggt aattcctccc ttttgttggt ttaatatcca
     4201 atgcttttac caaatcaatg taagtagcat caaaaccaaa tctcttttct tcctctgatt
     4261 ttttaattac atcaaatagt gatagaactt ctttgggtgg taaaaaaata gagttagcgt
     4321 ttattctggg agaaagttcg tatagctccg gtttaattag ctttttggta gttctaccaa
     4381 aagaaaacaa aactgaagaa ctatcttcta aagtcatact taacttaaga ggccgttcgt
     4441 ttggattttc ttttcctctt gtaactaaat caccaatttc ctctacttgg aacgtccaat
     4501 atagcttatc tcgcaattcc tcagaaaaat cacgaatatt ctttccttta tgagcttcct
     4561 catgactttt taaaacacta tataatgcct ttaaaataaa agttttccct ttgctatttt
     4621 ctgcaataat taaatttatt ttccctaaat tatcagcctt caataagcta atcggtccga
     4681 atttttcaag ctcaaatgat ttaatcatat aatcccccta atatatatca acaatatttg
     4741 tacaagccac attgtaaaaa gacagcaacc acagcaccat agtaaacgca tagcaggcgg
     4801 ataatggagc gacatagcgt ttcatgattt agctttctga ggtaggcaca ggctgctttt
     4861 ttcattttta ggcagcctac acaatcattt tacaaactgc ttcaaaaaat tcaaatcgtt
     4921 atcaaaaaac aagcgcaaat cgttcacgtt gtaacgcagc atcgcgaaac ggtcgagacc
     4981 gataccgaaa gcgaaaccgg tgtatttttc agaatcgata ttcacatttt tcaacacgtt
     5041 gggatgcacc atgccgcagc cgccgacttc cagccatttg ccgttttcgc ccataatgtc
     5101 gatttcggca gacggttcgg tgaacgggaa gaaagacggg cggaaacgta cttgcaaatc
     5161 atcgcgttca aagaagcgac ggataaaatc cgtgaacact gcttttaagt cggcaaaagt
     5221 tacgccctct tctacccaca aaccctcggc ctgatggaac ataggcgagt gcgtggcatc
     5281 gctgtccaca cggtaaacgc ggccgggggc gataatgcgg atgggcggct cttttttatc
     5341 gagcatatag cggatttgaa tcggggaagt gtgcgtacgc aaaacatcac cgttttcaac
     5401 gtaaaacgta tcctgcatcg cacgggcagg atggtttgca gggatgttca gggcttggaa
     5461 attgtgaaaa tcgtcttcga tttcaggccc gtccgccact tcgaaaccca ttccgtgaaa
     5521 gagttcgacc acacgttgca aggtcagggt tacgggatgc aggctgccgc cttcctgagc
     5581 gcgtccgggc agggtaatgt cgagggcttc ggcggcaagt cgggcttgca gcttggcttc
     5641 gttgagggca tcgcgtttgg cattaaaagc cgtctgaaac cggtttttgc attcattgat
     5701 atgcgcacct atggttttgc gctcttcagg cgacatttgc cccaaagttt tcagaagtcc
     5761 ggtcaactcg ccggttttgc caagataacg ggctttgatt tgttctagag cgttgaagtc
     5821 ttgcgcagct tctactgcgg caatgccttc tgcaacgatg cggtttacat tttccataat
     5881 atcaagtctg tcaattaatg atataccacc tgaaacaacg aggccgcctg tatagcctga
     5941 catatcagac tcttcagacg accttatttt cttcacggtc aatccattgg ataaattttc
     6001 t
//
LOCUS       DAXHRI010000125         5900 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXHRI010000125 DAXHRI010000000
VERSION     DAXHRI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.125"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            124..2142
                     /gene="uvrB"
                     /locus_tag="ACFM75_001662"
     CDS             124..2142
                     /gene="uvrB"
                     /locus_tag="ACFM75_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC6256174.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACDSIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQMKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKSRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKENLLF"
     gene            2335..2604
                     /locus_tag="ACFM75_001663"
     CDS             2335..2604
                     /locus_tag="ACFM75_001663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256175.1"
                     /translation="MFGLSILYKTYLEIDPKTISESNVANYVLFPNPEQITKEFCSSK
                     IDDDLMLDEEFIPIEIIGENELIPCGYLDFDIEYWSNYLFSEKNQ"
     gene            2697..2942
                     /locus_tag="ACFM75_001664"
     CDS             2697..2942
                     /locus_tag="ACFM75_001664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256176.1"
                     /translation="MHIYAGKCKKDDIGFFVEKEDKNVSFDCNGVSGGIVMRMIDGHS
                     EWVGIAVRANNDIIRFIPYYLVAKSIIQNILNRNQQA"
     gene            3061..3132
                     /locus_tag="ACFM75_001665"
                     /pseudo
     CDS             3061..3132
                     /locus_tag="ACFM75_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3606..4322)
                     /gene="trmB"
                     /locus_tag="ACFM75_001666"
     CDS             complement(3606..4322)
                     /gene="trmB"
                     /locus_tag="ACFM75_001666"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC6256177.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIALRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(4404..5846)
                     /locus_tag="ACFM75_001667"
     CDS             complement(4404..5846)
                     /locus_tag="ACFM75_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6256178.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLISLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALEWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLPEAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKHLERNHKHALHHAEAAAAERHP
                     EGLRILGDIYRYGLGMTSDKEKALHYYRQAAEAGSLAAHQKLVSDSALNHPEQYSGIK
                     DSAIKRQRAEQLYQKAQALHYRLQCVPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGIAADYGKARKWFEQAAAQKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQMPSEQRYTYPAETKQV"
BASE COUNT         1428 a         1455 c         1499 g         1518 t
ORIGIN      
        1 ttgggtagcg attgggtttc agacggcata cattcaggat ttcaccgcct tatgccgtct
       61 gaaagacgtt tccgacccat ccctactaca cgctttgtcc gcaaacgcat cacaggcaag
      121 attatggaag tcattcgata ccccgattcc cccttcaaac tccaccaacc cttcccgccc
      181 gccggcgacc agcccaccgc cattgccggc ctgctcgaag ggctttcaga cggccttgcc
      241 tatcaaaccc tgctcggcgt aaccggttcg ggcaaaacct acaccatggc gaacgtcatc
      301 gcccaaagcg gccgacccgc catcatcatg gcgcacaaca aaacccttgc cgcccagctc
      361 tatgccgaaa tgcgcgagtt tttccccgaa aacgcggtgg aatatttcgt ctcctactac
      421 gactattacc agcccgaagc ctatgtgccc agccgcgact tgttcatcga aaaagacagc
      481 gcgatcaatg aacacatcga acaaatgcgc ctttccgcca ccaaaaacct gatgacgcgc
      541 gacgacgtga ttatcgtcgc caccgtgtcc gccatttacg gtatcggcga cccgactgag
      601 tatcaacaaa tggtgttgtc cgtcaaagaa ggcgacacca tcgagcagcg cgacatcatc
      661 gccacgctcg tttccatgca gtacgaacgc ggcgatttgg acttcaaacg cggcagcttc
      721 cgcgtgcgcg gcgacgtgat tgacgtgtac cccgccgaaa gctccgaaaa cgccttgcgt
      781 atcagcctgt tcgacgacga aatcgaccgc ctcgatatgt tcgacccgct ttcaggcagc
      841 cttatccaac gcgtcggccg ctacaccgtc ttcccgtcca gccactacgt taccccgcgc
      901 gacaccgtat tgcgcgcctg tgactccatc aaagaagaat tgcgcgaacg cattgatttc
      961 tttaccaaag aaaaccgtcc cgtcgaacaa caacgcatcg aacagcgcac ccgtttcgac
     1021 ctcgaaatgc tctacgaaat gggcttctgt aaaggaatcg aaaactactc ccgccacttc
     1081 tccggcaaaa aagaaggcga accgccgccc acgctgatgg actacctgcc cgacaacgcc
     1141 atcatgttca tcgacgaaag ccacgttacc gtcacacaaa tcggcggcat gtacaaaggc
     1201 gacgcatcgc gcaagcaaaa cctcgtcgat tacggcttcc gcctaccttc cgcccgcgac
     1261 aaccgcccgc tcaaattcca cgaatttgaa aaagtcatgc cgcaaaccgt cttcgtttcc
     1321 gccactcccg ccaaatacga agaagaacac gccggacaag tggtcgaaca agtcgtccgc
     1381 cccacagggt tggtcgatcc ccaaatcatc atccgccccg tcgccaccca agtcgacgac
     1441 ttaatgagcg aaatcaacga ccgaattcaa aaaggcgaac gcgtactcgt taccaccctc
     1501 accaaacgca tggcggaaca actcaccgac tattacagcg aactcggcat caaagtgcgc
     1561 tacctgcaca gcgacatcga caccgtcgag cgcgttgaaa tcattagaga tttacggctc
     1621 ggactgtttg acgtactcgt cggtatcaac ctcttgcgtg aaggcctcga catccccgaa
     1681 gtctccctcg ttgccatcct cgacgccgac aaagaaggct tcctgcgctc ccaccgcagc
     1741 ctgattcaaa ccataggccg cgccgcgcgc aacgtgaacg gcgtcgccat cctgtacgcc
     1801 gacaaaatca ccgactccat gaaagccgcc atcgacgaaa ccgaacgccg ccgcgaaaaa
     1861 cagatgaaat tcaacgaaga acacggcatc gtgccgcagc agattaaaaa acaggtcaaa
     1921 gacattatcg acggcgtgta ccacgaagaa gacagcggca aaagccgtct gaaaggtaaa
     1981 aacaaggtta aagtaggcga aatccacaac gaagaagatg cgattaaaga aatcgccaaa
     2041 ctagaaaaag ccatgcaaca ggcggctagg gatttgcagt ttgaagaagc ggcggtgttg
     2101 agggatagga ttcgggggat taaggagaat ttattatttt aggatgtaaa aaatgattaa
     2161 gatgaaagat atttatgagt caatttgttg taaatctatt agaaagataa tgtatatatc
     2221 agatagtttt gtagctattt caggaatggg gtttattatt aaatttcagg aaaaatttta
     2281 tttttgctgt ttatatcatt gcatgtctaa tgaaggaaaa tttgaaaaaa taaattgttt
     2341 gggttaagta ttttatataa aacatatctt gaaatagatc ccaaaactat atctgaatct
     2401 aatgtagcta attatgtact ttttcctaat cctgagcaaa ttacaaaaga gttttgtagt
     2461 tcaaaaattg atgatgattt aatgcttgat gaggaattta tccctattga gattattgga
     2521 gaaaatgaac ttataccatg tgggtatttg gattttgata tcgaatattg gagtaattat
     2581 ttgttttcag aaaaaaacca atagaaaatg aaattctttt agtatgcggg ttcccttttt
     2641 cagataatga aatagattat actgatgatg gtgagattaa aaaagtatcg catcaagtgc
     2701 atatatatgc tggtaaatgt aaaaaagacg atataggctt ttttgttgaa aaagaagata
     2761 aaaatgtttc atttgattgt aatggtgtta gtggtggaat tgtgatgaga atgatagatg
     2821 gacacagtga gtgggttgga attgctgtta gggcaaataa tgacatcatt cgttttattc
     2881 cttattatct cgtagcgaaa tcaataatcc agaatatctt aaatagaaat caacaagcgt
     2941 aggtcggata cttgtatccg acaaaacctt caacattccc gtcgttgcaa tccattgcag
     3001 ctatgtccga aatgtcggat tcgaaaatcc gacctacaaa atcattccga gcataacact
     3061 atgaaataac gtcgttttta ccgcaatgac ggcacttact tttttacggt tgcaaccaat
     3121 aaacggcagc aagcgtaggg tgggcacttg ttgcccacgc gtttatctcc gaaaggcagc
     3181 aactgtaggt cggatacttg tatccaacaa acctttaaca ttctcgttgt tgcaatccat
     3241 tgcagcaatg cccaaaatgt cggattcgag aatccgacct acaaaatcat tccgaacata
     3301 atactatgaa ataccgtcgt tttaccgcaa tggcggcact tactttttta cggttgtaac
     3361 cagcaagccg tagcctacat cacaccaacc gcgtgcgtag ctatcacata ctacctgcgg
     3421 gcggcacaaa ctttaaaacc gatgtgcata aaacctacta aggatgcgtg cggtacgcag
     3481 gcgtttttgc cgttcaaacc attgttttaa accttgtatc gaaacatcac tcgctgcttt
     3541 atcttgaaaa aatgccgtct gtaagcaaac atgcttcaga cggcattttt ttatccggcg
     3601 gtttgttatc cgatccgttt gaataccaag tcccaaacgc cgtgtccgag gcgtttgccg
     3661 cgcgcttcga atttggtttc ggggcggtag tccggggtgg gggcgtagtc tgccgccgta
     3721 ttttgcaggc tgtcgaagct gctgaggact tcaagcatct gctgcgcata ttcttcccag
     3781 tctgtcgcca ggtggatata gccgccggtt ttgagtttgg gcagcagttt ggcgatgaag
     3841 ggggcttgta tcagacggcg tttgtggtgg cgttttttgt gccacgggtc ggggaagaat
     3901 atgtggatgc cgtcgagcga gccgtcttgc agcatatttt cgacaacttc tacggcatcg
     3961 tgccgcatca cgcggatgtt ttccaaatga ttttcgtcta tgagtttgag caggttgcct
     4021 acgccgggac cgtgtacgtc gatggcgagg aagtcggttt cgggcaggcg gagggcgatt
     4081 tctgcggttg ccgtccccat accgaagcct atttcgagga ttttgggtcg gttgcttccg
     4141 aaattggcat caagatcggc cggtgttgct tggtaatcga tgccgaagct gtcccataag
     4201 gtatcgatgg cgcgttgctg tgcggcggtc atatggcttt ggcggaggac gaagctgcgg
     4261 atgctgcggg gatgttcggc ttgccagttg ttttgtgttt ggttttcggc ggtgtctgtc
     4321 ataatgcttt cgtgcgcttg tgcaatcagg atgccgtctg aatgtgaaat gcctgttcgg
     4381 acggcgggaa gggaaagggg cgattatacc tgtttcgttt cggcagggta ggtgtaacgc
     4441 tgttcagacg gcatttgcca ttgttgcagc agttcttgca ggacgctttt ttgcccgtat
     4501 ccgttgttta tggcttcttg caggtagcgg caggcttttt ctttgtccgg cccgacaccg
     4561 tgtccgctgt aatggatgca ggcgaggttg tagaacgcca tactgtcttt ttgcgcggcg
     4621 gcttgttcaa accatttgcg ggctttgccg tagtcggcgg ctatgccctg tccgaagtag
     4681 tacatactgc ccagattggt ttgggctttg ctgtgtccga gttctgcggc ttctgtgtag
     4741 agtttgagcg cggctgcgta ttcgggcacg cattgtaatc tgtaatgcag ggcttgggct
     4801 ttttggtaaa gctgttctgc cctttggcgt ttgatggcgg aatctttgat accgctatat
     4861 tgctcaggat ggtttaacgc gctgtcggac acgagtttct gatgtgcggc aaggctgcca
     4921 gcttcggctg cctgccggta ataatgcagg gctttttctt tatcggaagt catgcccaat
     4981 ccgtagcggt agatgtcgcc cagtatccgc aaaccttcgg gatggcgttc ggctgcggct
     5041 gcttcggcat gatgcagggc atgtttgtgg tttctttcca gatgcttgcc ttgtaggtag
     5101 atgtcggcca gtgcggcatg ggcatcgggg tcattttcct gtgccgcctg ttggaaccgg
     5161 taaacggctt catcgggagc cgtaggtagg atgagttcgg caagcggggt gtaggcggga
     5221 atgtatcctt gttgcgcggc ggcgcgcaaa tgattgacgg cctgtgccgt gtcgactttc
     5281 gtcccttgcc cgaaacgata ttgcagcccg agctgccaat gtgcctccgg caagccttgt
     5341 gccgccgctt ttcggtagag caggtgcgcc gtttcaaaat ccggcggtgt gccgtaccgg
     5401 cagtattggg caagcaggta ttgcccgtgg gggttgttgt tttttgcgct tgtttttgcc
     5461 cattccaagg cttcttcaaa atgccgggac gcatacagca ggtacatcag gcggacttgt
     5521 gcttcaggca gcccttcctc tgctaatggc ctgtaaagcg atatcagggt ttccgggggg
     5581 gcgccttcca attcttggat atatgccaga ttgtaacgcg catagggatg cccctgttca
     5641 gcggcgatgc ggagcatttc cataccttgt tcgcggtctt ggtaggggct gtccgcgtcc
     5701 atcaggtgtt ttgccaactc gaatgcggcg gcgggatgac ctgccgaggc cgcttctgtc
     5761 agcagtcggc ggacttcggt aaagttatat tgtttttgag acaggcgttc taaggcctgc
     5821 cgatagggta agtcgggatt gttcatgtca gatcgttttc cgttttgtcg aatcatcgtt
     5881 attgtgttat gaagttaggt
//
LOCUS       DAXHRI010000126         5883 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXHRI010000126 DAXHRI010000000
VERSION     DAXHRI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.126"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(22..425)
                     /locus_tag="ACFM75_001668"
                     /pseudo
     CDS             complement(22..425)
                     /locus_tag="ACFM75_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(731..1990)
                     /gene="rho"
                     /locus_tag="ACFM75_001669"
     CDS             complement(731..1990)
                     /gene="rho"
                     /locus_tag="ACFM75_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC6256179.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(2209..4593)
                     /gene="ppsA"
                     /locus_tag="ACFM75_001670"
     CDS             complement(2209..4593)
                     /gene="ppsA"
                     /locus_tag="ACFM75_001670"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC6256180.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISEALKQLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            5004..5825
                     /locus_tag="ACFM75_001671"
     CDS             5004..5825
                     /locus_tag="ACFM75_001671"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC6256181.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKACAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1276 a         1366 c         1655 g         1586 t
ORIGIN      
        1 ttatggggct gtcctagata actaggataa actcgatttt actaattgtt ttaaaatgga
       61 acaagaactt ttatctcact gttgttaaaa cgccgttcgt acccctttaa atacagctca
      121 aaatgcgctt tgggaatgcc gttaaacttg cgtaaatgac gttttgcccg gttccaaaag
      181 ttctcaattc cattaatatg gttttgtcgt tcagcaaaat aactttcatc tgcttctact
      241 tcgccatcaa acatttccaa atgtggactg ttttgataaa taagtaatcg taaacgatga
      301 aaataatagg ctgcggtact tttattaacg cctactaact ctgctgctgt tcttgcagtt
      361 acacctgcga caaatagctc aatgagttta ttttgtttat accggcttag acgacttttt
      421 ctcataaagg caactctaac ttaatttgaa tttccctagt tatctaggac agccccaaat
      481 ttatacaaaa atgagcgcgg ttcggcgcaa ccttgaatca agttcccgca tcggttttca
      541 ttgccggtac ggatgcgttc aagccggctt tgcaaaggcc gcgctttcgg caagcggaca
      601 cggacactgc cgacggttgc gccgttaacg gggggaggga ggagctgcgc cgaccgtgtg
      661 aatgaaagtt ccgtctgaaa cccgattttc aggcttcaga cggcatttcg cattaatgcg
      721 ggcggcgcgt ttatttgccg cgcatcagtt caaagaaatc gtcgttgttt ttagaggctt
      781 tgattttccc gattaaaaat tcggctgcct cgatttcgtc catcgggtgc aggaacttgc
      841 gtaagagcca catacgttgt aactggtcgt ttgggacaag cagctcttcg cggcgcgtgc
      901 cggatttgtt gatgttgatg gcggggaaga ggcgtttttc cgccatacgg cggtcaaggt
      961 gcaattccat attgccggtg cctttgaatt cttcgtaaat cacatcgtcc atacggctgc
     1021 cggtttcaac caatgcggtg gcgatgatgg tcagcgaacc gccttcttcc acgttgcgcg
     1081 ccgcgccgaa gaaacgtttg gggcggtgca gtgcgttggc atcgacaccg ccggttagga
     1141 ttttgcccga ggtaggcacg acggtattgt aggcgcgggc aaggcgggta atcgaatcca
     1201 gcaggatgac cacgtctttt ttgtgttcta ccatacgctt ggctttttca agcaccattt
     1261 cggcaacttg gacgtggcgt tgcgccggtt cgtcaaaggt ggaggaaacc acttcgccgc
     1321 ggacggaacg gctcatttcg gttacttctt cgggacgctc gtcaatcaag aggacgatga
     1381 gttcgacttc gggatagttt gaggtaacgg cgtgggcaat gttttgcagc atcacggttt
     1441 taccgctttt gggcggggca accaagaggg cgcgctgacc tttgccgata ggggaaatca
     1501 ggtcgatggc acgtccggtc aggttttctt cggactttaa gtcgcgttcc agcttcaact
     1561 gttcggtcgg aaacagcggg gtcaggtttt caaacaggat tttatggcgg catacttccg
     1621 ggtggtcgcc gttgatggta tcaagcctga ctagggcaaa atagcgttcg ttgtcttttg
     1681 ggacgcgcac gcttccttcg atggtgtcgc ccgtatgcag gttgaagcgg cggatttggg
     1741 tgggtgagac atagatgtcg tcggggccgg caagatagga cgtgtccgcg ctgcggagga
     1801 agccgaagcc gtcgggcagg atttcaagcg tgccggagca ggtgaaaccc tcgccttttt
     1861 tcatcatctg gcggacgatg gcaaatacga ggtcttgttt gcggaatcgg ttggcgtttt
     1921 cgatgccgtg ttcttccgcc aattctaaga gtttggaaat gtgcagggtt tgtaattcgg
     1981 agacgtgcat aataatgatg tattttgaag aggaaaaaga caggcagatg ccgtctgaaa
     2041 gaagaagctg accgttgccg gttgctcggg gaaggggaat tgtaggcagt cggcgcgtgg
     2101 gtgtcaaata ttatcgcgga cggggcatcg gcaggaaatg ccgtctgagc ggagctgctt
     2161 ggaaaaaaat acccccgcgc ttttcaggct cgggggtatg ggcattgatt atttgttcaa
     2221 ttcattcgcc aaatatagcc aagtttcgat gacggtatcc gggttcaggg aaacgctttc
     2281 aatgccttcc tcaaccagcc atttggcgaa gtccggatgg tcggacgggc cttgaccgca
     2341 gatgccgaca tatttgttct gcttgcggca ggcggagatg gcaaggtgca gcatcacttt
     2401 gacggcaggg ttgcgttcgt caaacgattc ggataccaag ccgctgtcgc ggtcgagacc
     2461 gagggtcagt tgggtcatgt cgttcgagcc gatggagaag ccgtcgaagt attgcaggaa
     2521 ttgttccgcc aataccgcgt tgctcggcag ctcgcacatc ataatcaggc gcaggccgtt
     2581 tttgccgcgt tccaagccgt tttctttcag ggctttgaca acggcttcgg cttcgcccaa
     2641 agtgcggacg aacggaatca tgatttcaac gttggtcaac cccatttcat cgcggacgcg
     2701 tttcaaggct ttgcattcca aggcgaaaca gtctttgaag ttgtcggcga cataacgcgc
     2761 cgcaccacgg aagcccaaca tcgggttttc ttcatgcggt tcgtatacgt tgccgccgac
     2821 caggttggcg tattcgttgg atttgaagtc ggacatacgg acgatggttt tacgcggata
     2881 aaccgatgcg gccaatgtcg ccacgccttc ggcgatttta tcgacgtaga agtcgacagg
     2941 ggacgcgtaa ccggcgatac ggcgggtaat ttccgctttt aattcgtcgt cttgtttgtc
     3001 aaattccaac aaggctttgg ggtggatacc gatttggcgg ttgatgataa attccatacg
     3061 cgccaagccg atgccttcgc tgggcaggtt ggcgaagctg aatgcgagtt cgggattgcc
     3121 gacgttcatc atgactttta caggtgcttt aggcatgttg tccaaagcaa catcggtaat
     3181 ttgtacgtcc agcaggccgg agtagatgaa gccggtatcg ccttcggcac aggatacggt
     3241 aacttcttga ccgtttttca gcaattcggt tgcattgccg cagccgacaa cggcaggaat
     3301 acccagttcg cgcgcgatga tggcggcgtg gcaggtacgt ccgcccctgt tggtcacgat
     3361 ggcggaagcg cgtttcatca ccggttccca atccgggtcg gtcatgtcgg taacgagtac
     3421 gtcgccggct tcgacggaat ccatctcgga agcatcttta atcaggcgta ctttgccctg
     3481 tccgactttc tgaccgatgg cgcggccttc gcataatacg gttttttcgc cgttgatggc
     3541 gaagcggcgc aggttgcggc tgccttcttc ctgggatttg acggtttcgg gacgggcttg
     3601 caggatgtag agtttgccgt ccaagccgtc gcgtccccat tcgatatcca tcgggcggcc
     3661 gtagtgtttt tcgatggtca gtgcgtaatg cgccaactca gtaatttctt cgtcggtaat
     3721 ggagaagcgg ttgcggtctt cctcggggac atcgacgttg gttacggatt taccggcttc
     3781 tgctttgtcg gtaaaaatca ttttgatgtg ttttgagccc atggttttgc gcaggatggc
     3841 aggtttgcct gctttcagcg tgggtttgaa cacatagaat tcgtcgggat tgactgcgcc
     3901 ttgtacgacg ttttcgccca gaccgtagga tgaagtgaca aagacgactt ggtcgtaacc
     3961 ggattcggta tcgagggtga acatcacgcc ggacgcgccg ctgtcggaac gcaccatgcg
     4021 ttgtacgccg gcggaaaggg cgacgatgtc gtgttcgaag cctttatgta cgcggtagga
     4081 gatggcgcgg tcgttgtaca gggaggcgaa tacatggcgc atggcttctt taacattttc
     4141 caagccattg atattcagga aagtttcctg ttgtccggcg aatgatgcgt ccggcaggtc
     4201 ttcggcggtt gcggaagagc gtacggcaac agaaatgtcc gcgccgccgg catcggcaac
     4261 cattttgttc catgccgctt cgatttcggc atcgagctgt tcggggaacg gggtatccaa
     4321 aatccattga cggatttctt tacccacgcg tgccaattcg gcaacgtctt caacatccag
     4381 ttgtttcagg gcttcggaaa tacgctcgct cagaccattg tgagccagga aagcacgata
     4441 agcttcggct gtggttgcaa aaccgccagg aacgcgcacg cctttttcgg tcagctgact
     4501 gatcatttcg cccagcgagg cgtttttacc gcccacgcgt tcaacatctg tcatacgcag
     4561 gttttcaaac caaattacgt agttgtcggc catttgtgtg tccaatccaa aatatgttaa
     4621 aaaagaaaca aatccgcgtg cttattttaa gcgattcgtt cctctgctgt catgtgtttt
     4681 atccgtttta aaatcatgat gccgtctgaa aaattgcggt ttcggcgtgt gtagcggttt
     4741 gaaacttgca gccggtatac ttcttttttt gggtattttc tttgtaaaac aggtggtttg
     4801 aataggttaa tgttttttct gtttgatttt tttgtttatt ttttaaaatt ttctgccaaa
     4861 aaatacttta tataaataat tttatttcaa aattatattg tgtctgtttg ggtgtaatcc
     4921 gaggtaggcg tgctgcgggt gctttccttg tgtctgccgc tgctgttatg atgatatttt
     4981 tattctgtat ttaaggatgg aagatgagca gtccgcgcca tgtgttttac atttccgacc
     5041 gtaccggtct gactgccgag aatatcggcg aggcgttgct gaaccagttt ggcaatctgt
     5101 cgttcaaacg ccatacgcat ccgtttgtcg atacgccgga aaaggcgtgc gcagtggtgg
     5161 agaaggtcaa tcggagccgg caggaaaacg gtcagcgtcc gattgcgttt gtcagtgtgg
     5221 ttgatgacga aatccgtcgg attatcaaag gggcggatgc ttttcagatt aatttctttg
     5281 agactttttt gggactgttg gagaaagaac tcaataccga agccacggca tccgggcagg
     5341 ggcatcacag tatcggtaat acgaagcgtt acgatgcgcg tatggaggcg gtcaattttt
     5401 ctttgaacca cgacgacggg gtcagcgata agaaccttca ggaagcggat gtaatcttga
     5461 tgggtgtatc gcgttcgggc aaaacgccga cctgccttta cctcgccctg caatacggca
     5521 tccgtgcggc aaactatccg ctgattcccg acgatttgga atcggccgat ctgccgcgta
     5581 tggtcaagcc ttatagggat aagctgttcg ggttgaccat ccagccggaa cgtttgcagg
     5641 ccatccgcca agagcgccgc ccgaattcaa cttatgccaa aatcgataca tgccgcagcg
     5701 aggtggcgga cgcgcagagt atgttcagac ggcatggcat tccgtttgcg aatacgacgg
     5761 ataaatcggt tgaggaactg gcggtacaca tccttcaggc gtgcaagctc aaacgcaggt
     5821 tttgacgggc tttgattcgg tttgaaggcg gaactgccgt ctgaaatcag gtttcagacg
     5881 gca
//
LOCUS       DAXHRI010000127         5865 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXHRI010000127 DAXHRI010000000
VERSION     DAXHRI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.127"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(41..973)
                     /locus_tag="ACFM75_001672"
     CDS             complement(41..973)
                     /locus_tag="ACFM75_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6256182.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMPNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFSAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSGCKRREERDMAGLSERMAAELDRHG
                     AAVFFREYPNADHGNVSFYSLTDCVEYLRKAWQR"
     gene            complement(1043..3199)
                     /locus_tag="ACFM75_001673"
     CDS             complement(1043..3199)
                     /locus_tag="ACFM75_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC6256183.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTADLETVHIKGQRSYNAIV
                     TEKNGDYSSFAVTVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVRKRPTKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRHETFYAAADWDINPDTVLGAGYLYQQRHLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMNSHDVFADLKHYFGNGGYGKVGMRYSDRDADSNYAFAGSKLGMKT
                     PAGRPGCNTADDKACAVGLGTEIKQKALAFDASYSRPFRLGNTANEFVIGADYNRFRS
                     TNEQGRTTLYARGGLALNEFRSIPQVDLIANARKGVRGYSHTVATENLDEFGIYGKST
                     FHPADGLSLIGGGRLGHYKIESGEGKTLHKASKTKFTGYAGAVYDLNDNNSLYLSLSQ
                     LYTPQTNLDADGKLLKPRQGNQFEVGYKGSYMDDRLNARVSFYRMKDKNAAAPLNPNN
                     KKTRYAALGKRVMEGVETEISGAVTPKWQIHAGYSYLHSQIKTASNSRDDGIFLLMPK
                     HSANLWTTYQVTPELTIGGGVNAMSGITSSAGMHAGGYATFDAMAAYRFTPKLKLQIN
                     ADNIFNRHYYARVGGANTFNIPGSERSLTANLRYSF"
     gene            3359..4018
                     /locus_tag="ACFM75_001674"
     CDS             3359..4018
                     /locus_tag="ACFM75_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6256184.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     RLIGYSLPEIIRALLEMPSETAVADIARTYSAHYLNPNNRNMTLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGSYWLATACAGEHPSKPSPEMVFGICGELGLNPK
                     EALVVGDTAHDLHMAANAGAAAVGVTTGAHSREQLRTAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(4105..5073)
                     /gene="ispH"
                     /locus_tag="ACFM75_001675"
                     /gene_synonym="lytB"
     CDS             complement(4105..5073)
                     /gene="ispH"
                     /locus_tag="ACFM75_001675"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC6256185.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(5102..5629)
                     /gene="lspA"
                     /locus_tag="ACFM75_001676"
     CDS             complement(5102..5629)
                     /gene="lspA"
                     /locus_tag="ACFM75_001676"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC6256186.1"
                     /translation="MVLFYRLLKNMSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQ
                     YRERVNVIPSFFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEF
                     AALGKTGAAMIIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLA
                     VLDNIVHRKTQEEKY"
BASE COUNT         1223 a         1660 c         1620 g         1362 t
ORIGIN      
        1 tttccgatgc aaaacaaagc agtcatatat ttaattcccc ctacctctgc caagccttcc
       61 tcaaatattc gacgcaatcg gtcagcgagt agaacgagac attgccgtgg tcggcattcg
      121 gatattcccg gaaaaagacg gcggccccgt gcctgtccaa ctctgccgcc atgcgttcgg
      181 acagtcctgc catatcgcgt tcttccctgc gtttgcaacc gctaccccgt tcgagcgcgc
      241 cgatgttgag gcagacatcg atgccgttta gccggttttc agacggcata aaatcgagta
      301 tccgcctgtt gtgccaccaa atcgatgggg atacgagcca atgccgtctg aaacggcggt
      361 gggaaagcag ggaatacagt ccgaacagtg cgccgaacga gtgtccgaat acggcggttt
      421 cattgcggtt gagggtgtag cggctttcta aaaaggcggt cagctcgctg tcgataaagg
      481 cggagaagcg gtctgcctgt ccgaactgct gccgttcgtc tgctgtggcg ttgtctccaa
      541 gcggcggcgt gtagtcggcg gcacgttgtg ccaaatcgcg cacactgcct gtcgtgtagc
      601 cgataccgac aatcaggcag ggggcgttgc ttcgggtaac ggggttgggc atcagcgact
      661 gcatgatgtt gaaaagtgcg gggaaaaagg cttcgccgtc caaaacgaag aggacgggat
      721 agccttcaga cggcatttcg ccgagtgtcg ccgtctgaat ccgatagatt cgccccgtgc
      781 aggtggattt gatttcggtt tcaaaggctt ggggcagtat ggcaggttgg aatgtgtcgg
      841 tcggtatggg tttcatgatg ttcggcttgt gggtcagact ggcggcaagc ggtaaaaccg
      901 aaccggcaaa gcggtctgcc tgccggttcg gtctgttttc cttcgcaatg ccatactcca
      961 gttgtgagag catagggtat gcagcgcagc ttgttgtagt ttgatgatgc tgcggctgcc
     1021 gtttcagacg gcatattggt ctttaaaaac tgtaacgcag gtttgccgtc aggctgcgct
     1081 ccgaaccggg aatgttaaag gtgttcgcgc cgccgacgcg ggcgtagtaa tggcggttga
     1141 agatgttgtc ggcgttgatt tgcagcttca gcttgggcgt gaagcggtat gccgccatcg
     1201 catcgaacgt ggcataaccg cctgcatgca tccctgcaga tgaagtaatg ccgctcatcg
     1261 cgttcactcc gccgccgatg gtcagctcgg gcgtaacttg gtaagtcgtc cacaggtttg
     1321 cgctgtgttt gggcatcagc aggaagatgc cgtcgtcgcg tgaattggag gcggttttga
     1381 tttggctgtg cagatagctg taacctgcat ggatttgcca tttcggtgta accgcgccgc
     1441 tgatttcggt ctcaacgcct tccatcacgc gtttgcccaa tgcggcgtaa cgggtttttt
     1501 tgttgttcgg gttcaacggt gcggcggcgt ttttgtcttt catgcggtag aacgaaactc
     1561 gggcattgag gcggtcgtcc atgtagctgc ctttgtaacc gacttcaaac tggttgcctt
     1621 ggcgcggttt gagcagcttg ccgtcggcat cgaggttggt ttgcggtgtg tagagctggg
     1681 acaggctcag gtagaggctg ttgttgtcgt tcaagtcgta aaccgcgcct gcgtagccgg
     1741 tgaacttggt tttgctggct ttgtgcaggg ttttgccttc gcccgactcg attttatagt
     1801 gtcccaaacg tccgccgccg ataagcgaca gcccgtcggc aggatggaag gtggatttgc
     1861 cgtaaatgcc gaattcgtcg aggttttcgg tagcgacggt atggctgtaa ccgcgcacgc
     1921 ctttgcgcgc gttggcaatc aaatcaacct gcggtatgct gcggaactcg tttaaagcca
     1981 ggccgccgcg tgcatataaa gtagtacggc cttgttcgtt ggtgctgcgg aagcggttgt
     2041 aatcggcgcc gatgacaaat tcgttggccg tattgcccaa gcggaaaggc ctgctgtagc
     2101 tggcgtcaaa cgcgagggct ttttgtttga tttctgtacc caaacccacc gcgcaggctt
     2161 tgtcgtcagc cgtattgcag cccgggcggc ctgccggggt tttcatgccc agcttgctgc
     2221 cggcaaaggc atagttggag tcggcatcgc ggtcggaata gcgcataccg actttgccgt
     2281 agccgccgtt gccgaagtaa tgtttcaaat cggcaaacac gtcgtggctg ttcattttaa
     2341 atttgttcca atccgcgccg acaaatacgt gttgcggcag ggacggtaat ttgttattgg
     2401 catcggctgg caagccgttg tacggcgcga ggtggcgttg ctggtaaaga tagcccgcgc
     2461 ccaaaaccgt atcggggttg atgtcccaat ccgccgccgc gtagaaggtt tcgtgccggt
     2521 tgtttttctc ggcgggacgc ggagacgcgc cgacggtctg cgccatcacg cggccgcgca
     2581 cgctgccgtc tgaattgagg ctgcccgata cgtccgcctc ggctttatat tgtttgtgcg
     2641 taccgaaccc tgccgcagca tgaccttgga acgctttggt cgggcgtttg cgcaccagat
     2701 tcacgatacc gcccatctcg ccgctgctgt cgaacagtcc gctcggcccg cgcatcactt
     2761 ccacgcggtc gaaggcgaac agattgggca gcgtgccgtt gatactctgc atctgcgcgg
     2821 gcaggccgtc gatgttgtat tcgctgtatt cgtaaccgcg cgcgtaaacc gaagagcgtc
     2881 cgtcatcgtt gctcaacacg cgcaggccgg gcgttttgcg cgccaactgg tcaaacgtat
     2941 caacattgcg gtctttgacc tgctggttgg tgatgatact gacggattgc ggaatttcgc
     3001 gcaaagaagc ggggattttt gtgccgacgg tgacggcaaa cgagctgtaa tcgccgtttt
     3061 tctcggtgac aatcgcgttg tacgaacgct gccctttgat atggacggtt tccaaatccg
     3121 ccgtttgtgc ggcaaaaacc gaagacgaaa gtgctgccaa aaccgtggcg gcggtcatat
     3181 tgatgcggaa aactgacata aactgtccca ttcacataaa tgataatggt tctattttaa
     3241 taaagcacaa cgcggcttgt tcggaaaaac atatcgcgca gccgacaaat tttgtcgaaa
     3301 atgcgacacg tctgtgtttt ccgcataaaa tctgactttt caccgcaaac aacctgccat
     3361 gaccccgccc aaactcatca tcttcgactg ggacggcacg cttgccgata cgacccaacc
     3421 catcatcgac acgatgcgcc gcagcttcgc cgaatgcggt tttccgccgc ccgaagcgga
     3481 gcgcgtccgc aggctgatcg gatacagcct gcccgaaatc atccgcgccc tgctcgaaat
     3541 gccgtctgaa accgccgttg ccgacatcgc gcgcacttat tccgcacatt acctcaatcc
     3601 caacaaccgc aatatgacgc tctttcccga tgccctgccc tgtctggaca agctcaaagc
     3661 acaaggatac tggcttgccg tcgccacggg caaagggcgg gcgggtttgg acaacgccat
     3721 cagccaaacc gccaccggca gctattggct cgccaccgcc tgcgcggggg aacatccctc
     3781 caaaccctcg cccgaaatgg tattcggaat ctgcggcgaa ctgggactca atccgaaaga
     3841 agcattggtc gtcggcgata cggcgcacga cctgcatatg gcggcaaacg cgggggcggc
     3901 ggcagtcggc gtgaccaccg gcgcacattc gcgcgaacag ctccgcaccg caccgcatct
     3961 cgccgtattg gacggtttgt ccgaactgcc cggttttctt gcacaacatt acgcctgatt
     4021 ggcttccgca tccggcacgc ggcaaaaatg ccgtctgaag cctgttcaga cggcatttgt
     4081 tttgcccaaa cattcaacgc ttgctcaacg tttgcacaaa tcgggtttgg tttcgccctc
     4141 gcggcgcaac tctttgggca ggacgaacac gatgctttct tccgcgccct ccccttcgcg
     4201 cacggtttcg tgcccccatc cgcgtatggt tgccaatact tcccgcacca acacttcggg
     4261 cgcggacgcg cctgccgtta cgccgacttt gtttttgccc tcaaaccatg cgcgttgcag
     4321 gtagcctgca ttatccacca tatacgcatc gattccgcgc gatgccgcca cttcgcgcaa
     4381 gcggttgctg ttagacgaat tgggcgaacc gaccacaatc acgatgtcgc actgttctgc
     4441 caactctttg acggcggttt gccggttggt cgtcgcatag cagatatctt ccttgtgcgg
     4501 attgcggata ttggggaaac gcgcgttcag cgcggcgatg atgtctttgg tttcatcgac
     4561 cgagagcgtg gtttggctga cataggcgag tttgtcgggg tttctgactt cgagttttgc
     4621 cacatctccg accgtttcga ccaaaagcat tttgcccggc gcaagctgcc ccatcgtgcc
     4681 ttcgacctcg acgtgcccct tatgcccgat catgatgatt tcacagtctt gggcatccag
     4741 tcgggcgact tccttatgca ctttcgtcac cagcgggcaa gtcgcatcaa acacgcggaa
     4801 accgcgctcc gccgcttctt gctgcaccgc cttcgatacg ccgtgtgccg aataaaccag
     4861 cgtcgcgccc ggcggcactt ccgccaagtc ttcgataaac accgcgcctt tttcgcgcag
     4921 gttgtccacg acgaatttgt tgtggacgac ttcgtggcgc acataaatcg gcgcgccgaa
     4981 ttcttccaaa gcacgctcga caatactgat tgcccgatcc actccggcgc agaagccgcg
     5041 cggattggca aggataatgg ttttctcgtt cataagcccg gtatttcgtt ttcagacggc
     5101 atcaatattt ttcttcttgg gttttacggt ggacgatgtt gtccaacact gccaacaccg
     5161 caccgacgca gataaagctg tcggcaatat taaaggcggg ataaaaccaa ttttgccaat
     5221 aaaacaataa gaaatcgacg acatgaccgt gtatcaggcg atcgatgaca ttgcctaacg
     5281 caccgccgat aatcatcgcc gcacccgttt tgccgagggc tgcaaactcg tcgcgcaaga
     5341 tggcgcgtac caaatacgcg ctcaccgcca ccgccagcac caaaaaaaag tatttctgcc
     5401 agccgccctg atcggcaagg aagctgaacg ccgcgcccgg gttgtacacc agcgtcagat
     5461 cgaaaaagga aggaatgaca ttgacgcgtt cccgatactg aaacgacgac agcaccgccc
     5521 acttcgacca ctggtccagc acgatggcgg caagtgccaa tacccaatag cgcgttttac
     5581 ttgaaacaga tgaagacata tttttcaaca gccggtaaaa gagtaccatt ttacccgaaa
     5641 accccctttc ctgtacccga aacggcaaat gccgtaatct taaaacccgt cattcccgac
     5701 aacaccgtaa tctcgaaacc cgtcattccc gcgtaggcgg gaatccaaac ctgtccgcac
     5761 agaaacttat cggataaaaa cagttgctca aacccctcgt tctagattcc cactttcgtg
     5821 ggaatgacgg ttcagttgcg ttccaacaac accgcaatct cgaaa
//
LOCUS       DAXHRI010000128         5841 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXHRI010000128 DAXHRI010000000
VERSION     DAXHRI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.128"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(109..1026)
                     /gene="thrB"
                     /locus_tag="ACFM75_001677"
     CDS             complement(109..1026)
                     /gene="thrB"
                     /locus_tag="ACFM75_001677"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC6256187.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSR
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEVLKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQEGEMTFIKDPNAFRNLLLSLG"
     gene            complement(1063..1791)
                     /gene="ubiG"
                     /locus_tag="ACFM75_001678"
     CDS             complement(1063..1791)
                     /gene="ubiG"
                     /locus_tag="ACFM75_001678"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC6256188.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAEFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1943..3184
                     /locus_tag="ACFM75_001679"
     CDS             1943..3184
                     /locus_tag="ACFM75_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC6256189.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFAGSLIVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGNALGGDVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWMGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIINIAAQVLSQMELVPVFKG"
     gene            3317..3880
                     /gene="gmhB"
                     /locus_tag="ACFM75_001680"
     CDS             3317..3880
                     /gene="gmhB"
                     /locus_tag="ACFM75_001680"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC6256190.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            3909..4652
                     /locus_tag="ACFM75_001681"
     CDS             3909..4652
                     /locus_tag="ACFM75_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6256191.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            4649..5338
                     /locus_tag="ACFM75_001682"
     CDS             4649..5338
                     /locus_tag="ACFM75_001682"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6256192.1"
                     /translation="MTAFVHTLSDGMELTVEIKHRAKKNLIIRPIGTHTVSISVPPHL
                     SAAALNRWLRDNEAVLRRTLAKTPPHNTANRLPEHIWFHGRQLALTTHQDTQILLMPS
                     EIRVPEDTHDKQAALLATFLKRQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGVC
                     RKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQWL
                     KIHGRELFALG"
     gene            5378..5563
                     /locus_tag="ACFM75_001683"
     CDS             5378..5563
                     /locus_tag="ACFM75_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256193.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT         1366 a         1899 c         1383 g         1193 t
ORIGIN      
        1 ctataaaatg gaaaataccc ggctatcgtc tcattttcgt tttaatcagc cataaaaatg
       61 ccgtctgaaa ccctttcaga cggcatttct gtcaaacgcc ggacgcactc aacccaaact
      121 caacagcagg ttgcggaacg cgttcgggtc tttgataaac gtcatctcgc cctcctgcgg
      181 aaaatgaaaa tccaacaggc gcgacaccca aaaacggatg cagccggcac gttgggcggt
      241 cgggaaatac gccttttctt cggcactcaa ggggcgcacg ccctcataac cgccgataaa
      301 cgcctttttc aacacctcat ccagcttatt gtccgccgtc cttgcccaat cgttgaccgc
      361 aatcgccaag tcatacataa aattgccccg gcaggcataa tagaaatcga tgaaacccga
      421 tacctgaccg ccgtcaagca acacattgtc cttgaacaaa tcggcgtgga tgatgcccga
      481 aggcagatga ttgccgagat tgtccttcag cgcgtcgatt tcggaacaca gcagcgcggc
      541 atcgtctcgc gacaacacgg gcagcagtcg ggcgcacgcc tccgtccacc acgcatcgta
      601 acgcgggttt tccatttcca aagggaaatc ggcggcggca aggtgcattt tcgccaacat
      661 cgcaccggta tggaaacact gctccgccgt cggcagcgcg gtatccgaac ctttcaggca
      721 ggcaaccagg caggcgggct tgcccgccaa aacggaatca agccgaccgt ctttgcgcgc
      781 aaccggcgcg gcaaccgcca cgcccttcat actcaaatgc cggttaagct ccagaaaaaa
      841 cggcagctct tcctgtttca acacttcaaa cacggtcagc acataacgtc ccgaagtcgt
      901 cgtcagaaaa taattgctgt tggtaatccc ctgcgcgatg ccctgcaggg aaacaaattc
      961 ccccaaatcg taaccgctca ggaagccgcg catttcatca tcggaaacac tggtatagac
     1021 agacatatca actcacttgc tcaaaaacgg gcaaacccgc cgtcagaacg ccggacggca
     1081 ggcaaacata taattcacat cggtcgaatc gcacagggca taagtttgcg acaaaacatg
     1141 gtaagtcata cccttcgtat ccgccacatc caagcccgcc tgacggcaca tccgcgccag
     1201 ctcggcaggc gcgatgaatt ttttccagtc gtgcgtgcct ttggggacaa acttcaacag
     1261 atattccgcc gccacaatca gatgcaggta cgatttcggg tttttattga tggtggaaaa
     1321 aaacaccatg ccgtccggtt tgaccagatt ggcacaagca cgcacgatgg cggcgggatc
     1381 ggggacgtgt tccatcattt ccatgcacgt taccacatcg aacgagtgcg gttccgcctc
     1441 ggcaaggtct tccacgcgga tacattcgta ttcgatatcg gcgacattgt tcaaagccgc
     1501 gtgcaggcgg gcggtttcca gcgactgctc cgccatgtcg atacccttta caaactccgc
     1561 gccgcgccgc gccatacttt ccgccaagat gccgccgccg cagcccacgt ccaaaacccg
     1621 tttgccgcgc aaatccgcgt gtccgtcgat ataatccagc cgcagcggat tgatgtcgtg
     1681 caaggttttg aactcgcccg acttgtccca ccatttgtcg gcaatccggc tgaatttggc
     1741 gatttccccc tcatcgacat tatatttttt gtcggacatt ttccctccca tctgacgaac
     1801 cgcccactcc aaaacccaag attatactat ggaacacaat gccgtctgaa agccttttcg
     1861 ggcggcgcgg cacaaatgcc ttacaaatcc ttacacttta cggcataatg gcggctcgct
     1921 ttttctggca gaaagacaaa atatgcccaa caaaacccct tccctgttcg gcggcgcgat
     1981 gattatcgcc ggtacggtca tcggcgccgg tatgctcgcc aacccaaccg ccacatccgg
     2041 cgtatggttt gccggctcgc tgattgtatt gctctacact tggttttcta tgctctccag
     2101 cggtctgatg attttggaag tcaacaccca ctacccccac ggcgcaagtt tcgacacgat
     2161 ggtcaaagac ctgctcggac gcggctggaa catcatcaac ggcatcgccg tcgccttcgt
     2221 tttatacctg ctcacctacg cctacatctt cgttggcggc gacctgaccg ccaaaggcat
     2281 cggcaacgcc ttgggcggcg acgtttcgct taccgtcgga caactcgtct tcttcggcat
     2341 tctcgccttt tgcgtatggg catctgcacg cttggtcgac cgattcacca gcgtcctcat
     2401 cggcggcatg gtattaacct ttatttgggc aaccggcggc ctgattgccg atgccaaact
     2461 gcccgtcctc ttcgacaccc aagcccctac cggcacaaac tactggattt atgtcgccac
     2521 cgccctgccc gtctgcctcg cttccttcgg cttccacggc aacgtctcca gcctgctcaa
     2581 atactttaaa ggcgacgcgc ccaaagtggc taaatccatc tggatgggca cactgattgc
     2641 gctggtgatt tacgtcctct ggcaaaccgc catccaaggc aacctgccgc gcaacgagtt
     2701 cgcccccgtc atcgccgccg aagggcaagt ctccgtcctc atcgaaaccc tgtccaaatt
     2761 cgcccaaacc ggcaatatgg acaaaatatt gtccctgttt tcctatatgg cgatcgccac
     2821 ctcgttttta ggcgtaacgc tcggactctt cgactacatc gccgacatct tcaaatggaa
     2881 cgacagcgtg tccggccgca ccaaaaccgc cgcgctgacc ttcctgccgc ctctaatttc
     2941 ctgcctgctc ttccccaccg gcttcgttac cgccatcggc tacgtcggcc tggcggcaac
     3001 cgtctggaca ggcatcatcc ccgccatgct gctctaccgt tcgcgcaaaa aattcggcgc
     3061 aggcaaaacc tataaagttt acggcggctt gtggctgatg gtgtgggtct tccttttcgg
     3121 catcatcaac atcgccgcac aggtattgag ccaaatggaa cttgtccccg tatttaaagg
     3181 ataaaggcaa aatgccgttt gaagcccgcc ggcggcttca gacggcattg cctcgttatc
     3241 acgcggcaca ggcgcatcac tgccgatatt tcagtataat gccacccccg acctgcccca
     3301 atccaaagga aacgcgatga aactcatcat tctcgaccgc gacggcgtca tcaatcagga
     3361 ccgcgacgac ttcgtcaaat ccgttgacga gtggatacct gtcgaaggca gcatggatgc
     3421 ggtggcattt ctgacgcagg caggctacac cgtcgccgtt gccaccaacc aatccggcat
     3481 cgggcgcaaa tattttaccg ttcaaaacct taccgaaatg cacgccaaaa tgcaccgcct
     3541 cgtccgtcag gcaggcggcg aaatcaacgg catctggttc tgcccgcaca ccgatgccga
     3601 caactgcaac tgccgcaagc ccaaaccggg tatgattgaa gacatcatcg gacgcttcaa
     3661 cgcccaagcc tcggaaacct ggctggtcgg cgacagcctg cgcgatttgc aggcaatcga
     3721 tgccgtcggc ggaaaacccg cgctggttct gaccggaaaa ggcaaaaaaa cgctctccca
     3781 acacggacac gaattacccg aacacacaca ggttttcgat accctgctcg atttctcaca
     3841 atacatcatg caggaaaaca ccgcaccgca agccgactga acataccgca ttccgacaag
     3901 gcaaaaccat gctcatcatc cgcaacctga tttactggct gatactctgt tccagcctga
     3961 ttttcctctt tccctttatg ctgctcgcct cgcctttccg ggacggggcg cacaagatgg
     4021 cgcgggtctg ggtcaaaatc ctcaacctct cgctcaaaca catcgtcggg ctcaaatacc
     4081 gcatcatcgg cgcggaaaac atccccgacc gccccgccgt catctgcgcc aaacaccaaa
     4141 gcggctggga aacgcttgcc cttcaggaca tttttccgcc gcaggtttac gttgccaaac
     4201 gcgagctgtt caaaatcccc tttttcggct ggggcttgaa actggtcaaa accataggca
     4261 tagaccgcaa caaccgccgc gaagccaacg agcagctcat aaaacagggg ttggcgcgca
     4321 aaaacgaagg ctattggatt accattttcc ccgaaggcac gcgccttgcg cccggaaagc
     4381 gcggcaaata caaactcggc ggcgcgcgca tggcgaaaat gtttgagatg gacatcgtcc
     4441 ccgtcgccct caacagcggc gaattttggc cgaaaaactc ctttctgaaa tatccggggg
     4501 aaatcaccgt cgtcatctgt ccgaccatcc cgcacgcaag cggcagcgaa gccgaattga
     4561 tggaaaaatg cgaacatctc atcgaaacgc agcagccgct catttccggc gcaggtccgt
     4621 ttgccgccaa aatgccgtct gaaaccgcat gaccgccttt gtccacaccc tttcagacgg
     4681 catggaactg accgtcgaaa tcaaacaccg tgccaagaaa aacctgatta tccgccctat
     4741 cggcacacat accgtcagca tcagcgtccc accgcacctg tccgccgccg ccctcaaccg
     4801 ctggctgcgc gacaacgaag ccgtcctgcg gcgaacactg gcaaaaacac cgccgcacaa
     4861 tactgcaaac cggctgcccg aacatatctg gttccacggc agacaacttg ccctcaccac
     4921 ccatcaagac acgcaaatcc tgctgatgcc gtctgaaatc cgcgttcccg aagacacaca
     4981 cgacaaacag gcggcattgt tggcaacctt tctgaaacgg caggcgcaca gttacctgat
     5041 tccccgcctc gaacgccacg cccgcaccac acaactgttc cccgcctcct cctcgctgac
     5101 ctctgccaaa accttctggg gcgtgtgccg caaaaccaca ggcatacgct tcaactggcg
     5161 gctggtcggc gcaccggaat acgttgccga ctatgtctgc atacacgaac tctgccacct
     5221 cgcccatcca gaccacagcc ccgccttttg ggaactgacc cgccgcttcg ccccctacac
     5281 gcccgaagcg aaacagtggc tcaaaatcca cggcagggaa cttttcgcct tgggctgacg
     5341 cggtcccggg ccggcccgcc gctttcagac ggcatccgtg ccggaacagg cacgcgcccg
     5401 cccgattcaa accgcgatga cgctttgccg ccggttcggg gcaggatggc ggcacacgcg
     5461 ccgtctgccg cgtttcattt cacaccgctc ttccgaaacc cgaaacccgc ccggtccgac
     5521 gtgcggtatg aaacgcttaa gctggcgcga agtcttttac tgatttgccc gcgaaaatgc
     5581 cgtctgaaag gttttcggac ggcatttttt tgcgtttccc aggatggcgg cggattcgta
     5641 aaaggcggtc agggtggatt gtaggatggg ttgagacctg ccgaatccgc cgcatctgcc
     5701 aaatctaccg ccgtcattcc tacgaaagtg ggaatctaga acgcggggtt aagaaaacct
     5761 gcatcccgtc attcccacga acctgcatcc cgtcattccc acgaaagtgg gaatctagtt
     5821 ttgtcggtgc ggaaacttat c
//
LOCUS       DAXHRI010000129         5796 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXHRI010000129 DAXHRI010000000
VERSION     DAXHRI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.129"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            119..1294
                     /locus_tag="ACFM75_001684"
     CDS             119..1294
                     /locus_tag="ACFM75_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC6256194.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGEGDDVVVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDDIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKEHIEAVLVEATR"
     gene            1342..3252
                     /gene="pglD"
                     /locus_tag="ACFM75_001685"
     CDS             1342..3252
                     /gene="pglD"
                     /locus_tag="ACFM75_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC6256195.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAFLTACLFFRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMAPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWRTVICGLAVYRPDDIAALIGRYGVEKILLAIPSATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVTPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            3308..4600
                     /locus_tag="ACFM75_001686"
     CDS             3308..4600
                     /locus_tag="ACFM75_001686"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC6256196.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     AEVNAVFADVFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAQEHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(4652..5488)
                     /locus_tag="ACFM75_001687"
     CDS             complement(4652..5488)
                     /locus_tag="ACFM75_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC6256197.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYAFLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
BASE COUNT         1353 a         1789 c         1489 g         1165 t
ORIGIN      
        1 cacccccgta cagaccgatt ttgacaacac ctgcggcgcg cgcccgattc ctcgaaacac
       61 gcccccttca gacggcatag ggtcggaaat gccgtctgaa cccgacggac aaaccatcat
      121 gttgaacact tccctttccc cgtggccctg cttcacccaa gaagaagccg atgccgtttc
      181 caaagtcctc ttgtccaaca aagtcaatta ctggacgggc agcgaatgcc gcgaatttga
      241 aaaagaattt gccgcctttg ccggcacgcg gtacgccgtc gccctttcca acggcacgct
      301 ggcactcgat gccgcgctca aagcaatcgg cataggcgag ggcgatgatg tggttgttac
      361 ctcgcgcacc ttcctcgcct ccgcgtcctg catcgtgaac gcgggcgcaa accccgtgtt
      421 tgccgacgtg gatttgaaca gccaaaacat cagcgcggaa accgtcaagg ctgtgctgac
      481 cccgaatacc aaagcgatta tcgtggttca ccttgccggt atgcctgccg aaatggacga
      541 catcatggct ttggcaaaag aacatgattt gtgggtaatt gaagactgcg cccaagcgca
      601 cggcgcaaaa tacaaaggca aatccgtcgg ctctatcgga cacgtcggcg cgtggtcgtt
      661 ctgccaagac aaaatcatga ccaccggcgg cgaaggcggt atggttacga ccaacgacaa
      721 aaccctgtgg gaaaaaatgt ggtcgtacaa agaccacggc aaaagctacg atgccgtgta
      781 ccaccgcgaa cacgcgcccg gtttccgctg gctgcacgaa agtttcggca caaactggcg
      841 tatgatggaa atgcaggcgg caatcgggcg catccagctc aaacgcctgc ccgaatggac
      901 tgcctgccgt caagcgcacg ccgccaagct ggcggaaagc ctaggcaaat tcaaaagtat
      961 ccgcttggtt gaagtcgccg actacatcgg acacgcgcaa tataagttct acgctttcgt
     1021 caaacccgaa cacctcaaag acggctggac gcgcgaccgc atcgtcagcg aactgaacgc
     1081 gcgcaaagtc ccctgctatc aaggcagctg ctccgaagtc tatttggaaa aatctttcga
     1141 cgacacgcct tggcggccga aagagcgttt gaaaaatgct gtcgaactgg gcgacaccag
     1201 cctgatgttc ttggtgcacc cgacgctgac cgacgacgaa atcgcgttct gcaaagaaca
     1261 catcgaagcc gtattggtag aagccacacg ataacccttc agacggcata tgccgcctga
     1321 aagcacatac cgccccacga tatgactctg gaaaccctga tcgccctgcc gcgcaacatc
     1381 aagaaaatct gtttcctcat acacgatttt ctgatgattt tcattgcatt ctggttcacc
     1441 caaagcctaa aggccgacta ctcgaacgaa tggttcgacc ccgccaactg gcagtctttc
     1501 ctattgaccg cgtttttgac cgcctgcctg ttcttcagaa tggggcttta ccgcgccgtc
     1561 acacgcttca tcagcttccg catcctctcc accgcgctgg caggcagcct cgcctccgcc
     1621 gtattgtttt tcctcaatac gctgatattt gaagaacggc tgcggctcgc cctgccgatt
     1681 gtctatttct tactgctgtt tgtttccgtg accggctcgc gtatggtttt gcgcggactg
     1741 ttgtccgaac accccaaaaa acagatggcc cccgtcatta tttacggcgc agggcaggcg
     1801 ggcagacagc ttctcgaggc cgtcaaacaa atgcgcgaat attccgccgc cgcctttgtg
     1861 gacgacgacc ccaaactgtg gcgcaccgtc atctgcggcc ttgccgttta ccgccccgac
     1921 gacatcgccg ccctcatcgg acgctacggc gtggaaaaaa tcctgctcgc cattcccagc
     1981 gcgactcagg aacaacgccg ccgaatcatc aacaaactgg aagcctatcc gtgcgaagtg
     2041 ttgaccattc ccggaatgaa agacctgatg gacgggaaaa tcagcatcgg cacgctcaaa
     2101 aaaatctctg tgtccgacct gctcgggcgc gattccgtca cacccgacga ccgcctgatg
     2161 agtgccgaca tcgaaggtaa aaccgtcatg gtaaccggcg cgggcggctc catcggttcg
     2221 gaactctgcc gccagattat ccgccgccgc cccgaaaagc tgctgctgtt cgagttatcc
     2281 gaattcgccc tgtacgccgt cgaaaaagaa ttgcgcgaaa cctgcatcca aaaacgcctc
     2341 gacaccgaaa tcctgccctt tctcggttcg gtgcaaaacc gcacgctgct cgaacacgtc
     2401 atgaccgcct tttccgttgc gaccgtctat cacgccgccg cctacaaaca cgtccccatg
     2461 gtcgagttca acaccgtcga aggcatacgc aacaacatct tcggcacact cgagtgcgcg
     2521 cttgccgcca cgacatcggg cgtggaaact ttcgtcctca tctccaccga caaagccgtc
     2581 cgccccacca acaccatggg tgccagcaaa cgcatggcgg aactctgcct tcaggcactc
     2641 gccgccgaac ccggacaaaa aacccgcttc agcatggtac gtttcggcaa tgttttaggt
     2701 tcgtccggct ccgttgtccc gctgtttgaa aaacagattg cagaaggcgg cccgcttacc
     2761 ctgacccacc ccgaaatcac acgttatttc atgaccatac ccgaagccgc ccaactcgtc
     2821 atacaggcag gcgcgatggg tacgggcggc gacgtattcg tcctcgacat gggtgaatcc
     2881 gtcaaaatca tcgaccttgc ccgccaaatg attaccctaa gcggcctcaa acccaacaca
     2941 cccgaacaac ccgacggcga catcgaaatc ctcattaccg gactgcgtcc cggagaaaaa
     3001 ctctacgaag agctgctcat cggcgacaac gtccgcaaaa ccggccatcc gcgcatcatg
     3061 accgccaacg agaccatgct gccgtggcac gagctctccg ccctgctcga ccgcatccgt
     3121 gcggcctgcg accgttacga ccagcaggca atccgcaccc tgctcatcaa cgccccgacc
     3181 ggctttgccc cgagcgacgg catctgcgac ctgctttggg tacgagaaac acacagaaaa
     3241 aatgccgtct gaaccttcag acggcataac gtacaaacca acctacctta cacacacgga
     3301 gtttgacatg cagttctcag cattcggcga aaaattcacg caacacagcg gcatcctcca
     3361 actgatggac gacctcggcg acgcgctcaa aagcgacaag cccgtcaata tgctcggcgg
     3421 cggcaatccg gcgcgaatcg ccgaagtcaa tgccgtgttc gccgacgtat tttccaaact
     3481 ggcagcagaa catgccgtcg aaaacatcgg caactactcc aatcctcaag gagatgccgc
     3541 gctgattgac gcgctgaccg ccttcctcaa ccgcgaatac ggctggaacc tgaccgccga
     3601 caatatcgcg ctgaccaacg gttcgcaaaa cgcgtttttc tatttattca acctcttcgg
     3661 cggcaaattc aacctttcag acggcacatc cgcagaaaaa gccattttgt tgccgctcgc
     3721 gcccgaatac atcggctatg ccgacgtgca tgtcgaaggg cagcacttcg tttccgtcaa
     3781 gcccaaaatc gaaaacgtcg aacacgaagg cgaagcaggc ttcttcaaat accgcgtgga
     3841 ctttgacgca ctggaaaacc tgcccgaact caaagcgggc aaaatcggcg cgatttgctg
     3901 ttcgcgcccg accaacccga ccggcaacgt gctgaccgac agcgaaatgg cgcggctgga
     3961 cgctttggcg caagaacacg ggattccgct gattatcgac aacgcctacg gaatgccgtt
     4021 ccccaacatc atttacagcg acgtaacgct gaattggcat gaaaacatca tcctctgctt
     4081 cagcctttcc aaagtcggcc tgccgggcgt tcgcaccggc atcatcgtcg ccgcgccgga
     4141 agtggtcaaa gccgtcagca gcctgaacgc gattgtgaac cttgccccca cgcgcttcgg
     4201 cgcggccatc gccgccccgc tactggaaag cggcgagatg aaacggcttg ccgaccaagt
     4261 catccggccg ttttaccgca atcaggcgca aaccgccgtc tcgctgctca agcgcgagct
     4321 gggcgcgtac ccgatgaaaa tccacaaacc cgaaggcgcg attttcctgt ggctctggtt
     4381 tgaaaacctg cccgtttctt cgcaaaccct gtacgaaatg ctcaaagccg aaggtacgct
     4441 gattattccg ggcgaacatt tcttcgtcgg catcgacacg caggattacc cgcacgcggg
     4501 cgagtgcatc cgcatgagca tcgcgcagga cgctcaaacg ctggaaaaag gcattgccgc
     4561 catcggcaaa accgtccgaa aactgtacga caacgtttaa accgcaaaaa atgccgtctg
     4621 aaggcctttc agacggcatt tttatccgca ttcaatgtcg ggaaaaatgc gcccacactg
     4681 gcctgcagtt ttccggcagt tcgggacacg cgccgaggat gccgccgctg aagtcgttta
     4741 aacggtggaa gtcgctgccc gcactggcga gcatgccgaa gcgttctgcc aaaaatgcgt
     4801 agttgaggcg gtcgtttttg cagcagttgc cgctgtggac ttcgatgccc gcgccgccga
     4861 ggtttttaaa ttcttcaaac agattgcgct tggcggtagc ggacaaatca tagcgcatcg
     4921 gatgggcgat gactgccatg ccgcccgcgc cgttgacggc ggagacgcag tcttccagcg
     4981 tcgcccattc gtgtcggacg gcgcaggatt tgccgtcgcc caagtatttg gtgaacgcct
     5041 gctgcttgtt tttgacgtgt cccacccgaa tgaggaactc ggcgacgtgt gtgcggctga
     5101 ccatttcttt gtttgccgcc agcgcgagcg cgccgtcgta tgcgcccgaa atgccgcgtt
     5161 tttccagctt ggcggcgatg gcttcgaggc gtttcagacg gccttgccgc acttgcgcca
     5221 acaggttttg caggttttcg tcttgttcgt caaagtctaa gccgacaacg tgtatggtac
     5281 gcccgcgcca cgttacggag atttccacgc cgttaatcag gcgcaaaccg agcctgtcgg
     5341 cttcggcacg cgcttcggcg atgccgccgg tgtggtcgtg gtcggtcaac gccagcagcg
     5401 tgcagccgtt ttgatgcgcg aggcgcacaa cttcggcggg ggaaagcata ccgtcggaaa
     5461 cggtggaatg gcagtgcagg tctatcatgg ggtgttatat gtgtggtgaa tgaaggtcgg
     5521 ggggcaatat tgtcgggcgg tgtggcggcg gcggtcattt taaaaaacag gtgtatggca
     5581 aatgcaaagg aaaagccgct atgccgtcat tccagcgcag gcgggaatcc agactttgat
     5641 ttattggaaa tgtttaaggt taaccgctat ttcaaacttc cggattcctg ctttcgaggg
     5701 aatggcgata tgaccgtttt caattttaat ctactataaa agactgtctg aaaacgtggt
     5761 tttatagtga attaaattta aaccggtaca gcgttg
//
LOCUS       DAXHRI010000130         5790 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXHRI010000130 DAXHRI010000000
VERSION     DAXHRI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.130"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            131..565
                     /locus_tag="ACFM75_001688"
     CDS             131..565
                     /locus_tag="ACFM75_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC6256198.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1441)
                     /locus_tag="ACFM75_001689"
     CDS             complement(578..1441)
                     /locus_tag="ACFM75_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6256199.1"
                     /translation="MELNEFFDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1449..2066)
                     /locus_tag="ACFM75_001690"
     CDS             complement(1449..2066)
                     /locus_tag="ACFM75_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC6256200.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2229..2843)
                     /locus_tag="ACFM75_001691"
     CDS             complement(2229..2843)
                     /locus_tag="ACFM75_001691"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC6256201.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQIDLQEG"
     gene            3049..4821
                     /locus_tag="ACFM75_001692"
     CDS             3049..4821
                     /locus_tag="ACFM75_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC6256202.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFSRFTQQTGTTVETAWENGTHLPTQDEQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LEIRSQPQQGTTVSLTGASEESLK"
     gene            4818..5474
                     /locus_tag="ACFM75_001693"
     CDS             4818..5474
                     /locus_tag="ACFM75_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6256203.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPMLE
                     "
BASE COUNT         1435 a         1637 c         1336 g         1382 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gtcagctttc ccacgccgcc acggcttata tccccccgaa
      241 cgattttcaa ccgaactgcg acatacgccg actcgggctg acccaaagtc agcacaatga
      301 gctgcgtaaa atccgcaccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gccggtctgt cgtcgaaatc atttcctcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accggttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca caacgcccgt tgtttcggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggattt
      841 ccccccgcat ccccgtcgta ccggcattct cgtcaaaata ttcgggcatc aggtgccgtc
      901 tgttggaagt cgcgtaaacc aaaacgttgg cgcaacgttg agacagaccg ccgtctaacg
      961 cggttttcaa tgccttatag gtttcatcgc cgctttcaaa cgacaaatcg tcgcaaaata
     1021 cgataaattt ttccggacgt tccttcaaaa gcgtcaacag gtaaggcagg ccgattaaat
     1081 cgcttttatc gacttcgatc aggcgcaatc ccttatccgc atattcgtgt agcagggctt
     1141 tgaccagcga ggatttgcct gttccgcgcg cgccgctcat caatacattg ttcgcgggtc
     1201 tgccgacaat gaactgttcg gtattacgca ccagcaattc ggtttgcctg ccgactcccg
     1261 ccagccttac caagggaaag gtgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc
     1321 ccgcactctg ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa
     1381 gcacggcatc caaacgccgc aaaacggcat aggctttatc gaaaaattcg ttcaattcca
     1441 tctctacctc actttgcata tctttgcgcc atcagccgtt cgacggtgtc gacgattgcc
     1501 tgcgtattcg gatcgatttc gatgttgatc ctgtcgccga cctttctgct gccgaacagc
     1561 gtccgttcca aagtttcggg aatcagatgg acattgaaac ggccgtcttc gactttgcct
     1621 atggtcaggc tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt
     1681 tcatgcggga gcgaaaacca aaccgtgcgg ttgaacccgt cccgttcgat ttcgacaata
     1741 ggcacggttg ccataatgtg tccgctcatg acgtgtccgc cgatttcgtc tccgaaacgc
     1801 gccgcccgtt cgatgttgac gcaatcgcct tccttcagca gccccaaatt ggtttttgcc
     1861 aaagtttccg ccattaaatc gaaactgacg cggtttcctt cgatttcggt aatcgtcagg
     1921 cagcagccgt tattggcgac cgatgcgccg cgttgcagat tgtccgccgc ctcttgcgga
     1981 agctcgacga cataagtgtg aaatgcctcc gacgggcggt ggattgccgt cagttttccc
     2041 aatccttgaa caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca
     2101 aattgtagca tctccccgcg aaaaatgccg tctgaaatgc cttcagacag cattatgcct
     2161 ccgattcggg caaaaaccgc ccggtatggc ttgacctttc ctttccatgc cggtcggcgg
     2221 tcttgccctt atccctcctg caaatcgatt tgcgtgttca agtcggcaaa atgcccgtca
     2281 aactcgaatc tgaccgaccg cgccctttgc tgctgcaacc agcttcttaa tgttttcata
     2341 cccgaaaaca gatagggaat cgcgctttgc agaatctgcg ggcggatata cataatgttg
     2401 tagtgcatcg ttatcggcgt ttccacataa tacgcattgc acaacggtgt gcgtttcgac
     2461 acggtttcaa acctcgccac caaatcgtcc ggcagatacg gcatatcgca cggcacaacc
     2521 aaaagccagt cagcagtcgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc
     2581 gggccgaaat gctgccactg ccgcgcatcg ggaaaaatat gcggacttct tcgagcatat
     2641 tcttccaaat tccggttggt gctgatggcg atatggctga cctgcggcct gaccctgtcg
     2701 atgacatggt ctatcagtgc cttacccccc aaaagagcaa gccccttgtc ctcgcctccc
     2761 atacggctcg cctgaccgtc ggccagtatc agggcaaaag ttttcattgc ggatgttctc
     2821 ttggaaaagt tcgaggtttt catgattgca gtctgccgtt cccaatgact caaaatgccg
     2881 tctgaagcag atggcaaata aattcatatt atctgaattt tatcataaca tgatttaaca
     2941 ctgaagcggt gcggattaag ttttccttaa ccaattcttt ctgagcagtt gtatctaatc
     3001 ccaaagaatg atattgtttg cattatttag aacaattttt cgccgagcat gatactgcca
     3061 gcccgttttt cagacggcat cagcctttcc ctacgcctga aactcctgac cggactgtgg
     3121 gtcgggttgg cggcattgtc tgtcgttttg acactgctgc tctctttgcg tctggaaaac
     3181 gcggcctccg tcatcgaaga ggcgggcaac ttgagaatgc aggcataccg tctggcatac
     3241 atggcgggtg aaggctcgcc ccgtgcgcaa attgacaatc aggttgccga atttgaaaaa
     3301 agcctgaatc gtatttcaca aagtgatgcc atccatccgc tgattccttc ggacacccct
     3361 cttgcttatg atttgataca atccatgctg attatagatt ggcaggcaca catcctcccc
     3421 ccgctccagt cctaccggcg accgactcag gtcgatctct accgctttgc cggaaacatc
     3481 gaactgtttt tgcaggcatt ggaaaatgcc aacgaaaaaa acacatggtg gctcaggcgt
     3541 tttcaatggg caattatgtt gatgacgctg gtgtcgtctg tactgatgct gttttggcac
     3601 cagatttggg ttatccggcc gctgcaggcg ttaagggaag gtgcggaacg catcggacgg
     3661 aggtgtttcg atattccggt tcccgaaggc ggtacgccgg aattcaaaca ggtcgggcgt
     3721 tgtttcaatc aaatgggcgg caggttgaaa attttatatg atgatttgga aggacaagtc
     3781 gccgagcaga cacgcagtct cgaaaaacaa aatcaaaacc tgaccctgct gtaccaaact
     3841 acacgggacc tgcaccaatc ctacataccg caacaggctg cagaacattt tctaaaccgt
     3901 atcctgcccg ccgtaggagc agattccggc agagtttgtt tggacggcgg atccgatgtt
     3961 tatgtttcca ttcatcatgc ggattgcggc acagcagctt cggatttggg gaagtaccat
     4021 gaggaaatct tccccattga gtaccagaac gaaacattgg gcaggctgtt gctcagcttt
     4081 ccaaacggca tttctcttga tgaagacgac cgcatcctgc ttcaaacact aggcaggcaa
     4141 ttgggcgtat cgcttgccgg cgcaaaacag gaggaagaaa aacgcctgct tgcagtattg
     4201 caggaacgca acctgattgc gcaaggatta catgacagca tcgcacaagc attaacgttc
     4261 ctaaacctac aggtacagat gctggaaacc gcctttgccg aaaacaaacg ggaggaagcc
     4321 gcagaaaaca tcggcttcat caaaacaggc gtgcaggaat gttatgaaga tgtccgcgaa
     4381 ctgctgctca acttccgtac caaaatcagt aataaagaat ttcccgaagc cgttgccgac
     4441 ctattctcgc gctttacgca acagaccggc acgactgtcg aaaccgcttg ggaaaacggc
     4501 acgcacctgc ctacacagga cgagcagctc caaatgattt tcatcctgca ggaaagcctg
     4561 tccaacatcc gcaaacacgc ccgcgccacc catgtaaaat tcaccctttc cgaacacggc
     4621 ggacgcttta ccatgaccat ccaagacaac ggacaaggtt tcgacacgga gaaaatagga
     4681 gaacctacgg gcagccatgt cggactgcac attatgcagg agcgtgccaa acgcatccgt
     4741 gccgttttag aaatccgttc ccaaccccaa caaggaacca ccgtctcatt gacgggtgca
     4801 tctgaagaaa gcttgaaatg actattaaaa ttattctgat agacgaccac accctcttcc
     4861 gcagcggcat taaagccctt ttgtcgcgcc aacacggttt tgaagtcatc ggcgaagccg
     4921 cagacggcct ctcgggcatt aaaatgatca accggctcca acccgatgtc gtcctgcttg
     4981 accttgatat gcccggtatg aacggacgcg aagcactctc ccaaatcatc agcatcaatc
     5041 cgcagcaggc agtgattatg ctgaccgttt ccgaagacaa cgacgacttg accgaatgta
     5101 tgcgcatcgg cgcgcgcggc tacctgctga aaaacatcaa cgccgacttt ctgctcgaaa
     5161 gcatacgcaa agccgccgaa ggcgataatg tattctcgcc cgagatgacc gccaaactcg
     5221 tcaaaagcct gatttccccc caacctgccc aagggacgca ggcactctcc tcacttaccc
     5281 ctcgcgaact ggaaatcttg ggctatctcg ccgcaggaca cagcaacaaa atcatcgccc
     5341 gccacctcga tcttgccgaa tccaccgtca aagtccacgt tcaaaacctg ctccgcaaac
     5401 tcaacctcag cagccgggtg caggccgctg tttacgccat ccggcacaac gtcccccaac
     5461 ccatgctgga ataaggcggg ttcagacggc atattagggg ttttaatccc cgtacggtca
     5521 ttcggataac agaccaagca tgtaagttta tgcccccata agtacgcttg gcatagcagt
     5581 aatattgttc ggttcagtgt tttccgtttg cccctatctg atactgcaat atcagctatg
     5641 ccgtctgaaa acgcatcatc atgatatttt cagacggcat aataaaaagc ggaaatacta
     5701 atgcagggta aaatgttcca ttccgaatcc cataaatata caatggctta tatcgtttaa
     5761 gcatgtgtaa cccaccctca tatcaatcaa
//
LOCUS       DAXHRI010000131         5786 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXHRI010000131 DAXHRI010000000
VERSION     DAXHRI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.131"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            406..5739
                     /gene="iga"
                     /locus_tag="ACFM75_001694"
     CDS             406..5739
                     /gene="iga"
                     /locus_tag="ACFM75_001694"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6256204.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYKPHKAWNASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRVGAGRQLVYEKGAYHQEGKEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQRDNAGTIKG
                     NGEHHWNITSGTNSKIGSTAVRLAGNEKDANNGQNVTFENNGTLVLDQNINQGAGGLF
                     FKGDYTVKGANNGITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQ
                     GQLKVGDGTVILNQQADADKKVQAFSQVGIVSGRGTLVLNSPDQINPNNLYFGFRGGR
                     LDANGNDLTFEHIRNVDEGARIVNHNTDRASTITLTGKSLITNPNSLSVHSIQNDYDE
                     DDYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQE
                     EAKKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGANLNGK
                     ISVTQGNVLLSGRPTPHARDFVNKSSARKDARFSKNNEVVFEDDWINRTFKATEITVN
                     QSASLSSGRNVSNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKAL
                     NSFGATQINGNVNLSQNAALTLGKAALWGQIQGQGNSSVSLNQHSKWHLTGDSQVHNL
                     SLADSHIHLNNASDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGH
                     YQLHVQDKTGEPNQEGLNLFDASSVRDRSHLSVSLANNHVDLGALRYTIKTENGITRL
                     YNPYAENRRRVKPAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQA
                     KAEQVKRQQAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHKKEREA
                     AELSAKQKAEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEE
                     ERRQTAQSQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQE
                     ERKAAELLAKQRAEAEREAQALAAHRKAEAEEAKRQAAELAHRQEAERKAAELSANQK
                     AAAEAQALAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQ
                     EARQTAELARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRK
                     RRAAPQDYMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIP
                     LDALEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQADAVSTNTN
                     SALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFS
                     SKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWY
                     VAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSA
                     DYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGN
                     SFVYKADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1944 a         1385 c         1302 g         1155 t
ORIGIN      
        1 tccatccgta cggaaacctg caccgcgtca ttcccacgaa cccacatccc gtcattacca
       61 cgaaagcggg aatctaggac gcagggttaa gaaaacctac atcccgtcat tccctcaaaa
      121 acagaaaacc aaaatcagaa acctaaaatc ccgtcattcc cgcacaagcg ggaatccaaa
      181 aacaaaaagc cgcagaaatt taacggaaga accgaacccc gaaaatcggc ttcccacctt
      241 cgcctgaata acagaacaaa ggaagcggaa ataaaaacat cgggattctt tttttaaatt
      301 ggcaataaaa caccaaaatt aatgatttac acaacaaaaa atagtcaaca ccgccaaacc
      361 ggcgtaaaat acaagacatt atcaccctta aaacggtaaa accttatgaa aaccaaacgt
      421 tttaaaatta acgccatatc cttatccatc tttcttgcct atgcccttac gccatactca
      481 gaagcggcat tggtcagaga cgatgtcgat tatcaaatat tccgtgactt tgcagaaaac
      541 aaaggcaaat tttttgtcgg cgcaaccgat ttatcagtga aaaacaaaca aggtcaaaac
      601 atcggcaacg cattatccaa cgtaccgatg attgatttta gcgttgcaga tgtcaacaaa
      661 cgcatagcaa cggtagtcga tccccaatat gctgtcagcg tcaaacacgc caaagcagaa
      721 gtccatactt tttattacgg tcaatacaac ggtcataatg atgtagccga caaagaaaat
      781 gaataccgcg ttgtcgaaca aaataactat aaaccccata aagcttggaa tgcgagcaac
      841 ttaggccgtc tcgaagatta taatatggcg cgttttaaca agtttgtaac tgaagttgcg
      901 ccaattgcac caactgatgc cggcggcgga ttggatacct acaaagataa aaaccgtttt
      961 tcctcttttg tcagagtcgg tgcaggcagg caattagttt atgaaaaagg ggcttatcat
     1021 caagaaggaa aggaaaaagg ctacgatttg cgcgatcttt cacaagccta tcgttatgcc
     1081 attgcaggta caccttataa agatattaat attgaccaaa caatgaatac cgaaggtttg
     1141 attggctttg gtaatcataa taaacagtat tcagcagaag aacttaaaca agcactctcg
     1201 caagatgcgt taacaaatta cggcgtgttg ggcgatagcg gctctccgtt atttgctttt
     1261 gacaaacaaa aaaatcaatg ggtctttttg ggaacttatg attattgggc agggtacgga
     1321 aaaaaatctt ggcaagaatg gaatatctat aaaaaagaat ttgcagataa aatcaaacaa
     1381 cgcgataacg ccggcaccat caaaggtaat ggagaacatc attggaacat tacttctggc
     1441 acaaacagca agattggttc gacagcggta aggcttgccg gcaatgaaaa ggatgcaaac
     1501 aacggacaga atgttacctt tgaaaacaac ggcactttgg tattggatca aaacatcaac
     1561 caaggcgcgg gcggtctgtt tttcaaaggc gattacacag tcaaaggtgc aaataatggc
     1621 atcacttggc taggtgcagg gattgatgtt gccgacggca aaaaagtcgt ttggcaagtc
     1681 aaaaatccga atggcgacag attggcaaaa atcggcaaag gcacattaga aataaacggc
     1741 acaggcgtta accaagggca attaaaagtc ggcgacggta cggttattct gaatcaacaa
     1801 gccgatgccg acaaaaaagt ccaggctttc tcccaagtcg gcattgtgag cgggcgcggt
     1861 acattggtgc tgaacagtcc ggaccagatt aacccgaata atctgtattt cggtttccgt
     1921 ggcggtcgtt tggatgccaa tggcaatgac ttgacttttg aacacatccg caacgtggat
     1981 gaaggcgcgc gcattgtcaa ccacaacaca gaccgcgcct ccacaatcac gctaacgggt
     2041 aaatctttaa ttaccaatcc gaacagcctt tcggtacata gtatacaaaa tgactatgat
     2101 gaagatgatt atagttatta ttaccgacca aggcgaccca tcccacaagg taaagatctt
     2161 tattacaaaa actatcgtta ttacgcccta aaatccggcg gcagcgtgaa cgcaccgatg
     2221 cccgagaacg gtgtaacaga aaataacgac tgggtcttta tgggatatac gcaagaagaa
     2281 gccaagaaaa acgcgatgaa ccacaaaaac aatcagcgta ttagcggttt tagcggtttc
     2341 tttggcgaag aaaacggaaa aggacataac ggcgcattaa accttaattt caacggcaaa
     2401 agcgcgcaaa accgtttctt gttaacaggt ggtgccaatt taaacggaaa aataagcgta
     2461 actcaaggca atgtcttgtt atcaggtcgt ccaacaccac acgcaagaga ttttgtgaac
     2521 aaatcttcag cccgaaaaga cgcacgtttc tccaaaaaca atgaagtcgt atttgaagac
     2581 gactggataa accgtacatt caaagccaca gaaattacgg ttaatcaatc cgcatcgtta
     2641 tcttccggca gaaatgtatc caacattacc gccaacatca ccgcaacaga caacgccaaa
     2701 gtaaatttgg gttacaaaaa cggcgatgaa gtttgcgtgc gctcggacta taccggttat
     2761 gttacctgca acacaggcaa cttatccgat aaggctttaa acagctttgg agcgacacag
     2821 attaacggca atgtgaattt gagtcagaat gcggcattga ctttgggcaa ggctgcatta
     2881 tgggggcaaa ttcaagggca aggaaacagc agcgtcagcc tgaaccaaca tagcaaatgg
     2941 catttgaccg gcgacagcca agtgcacaat ctgtcattgg cggatagcca tattcatttg
     3001 aacaacgctt ccgacgcgca aagtgcaaat aaataccaca cgctcaaaat caatcattta
     3061 tccggtaacg ggcattttca ttatctgacg cacttggcaa aaaatcttgg ggataaagtg
     3121 cttgtgaagg aatccgcatc cggtcattat cagctccatg ttcaagataa aacaggcgaa
     3181 cccaatcagg aagggctgaa tctctttgat gcatcatccg tacgagaccg atcgcacctt
     3241 tccgtttcct tggcgaataa ccatgtagat ttaggcgcat tgcgttatac aatcaaaaca
     3301 gaaaacggta ttacccgatt gtacaatcct tatgccgaga accgccgccg agtcaaaccg
     3361 gccccgtctc ctgccacaaa cacggcttct caagcacaaa cggacagtgc acaaattgcc
     3421 aagcctcaaa atatcgtcgt cgcaccgcct agcccgcagg caaatcaagc cgaagaagca
     3481 aaacgccaac aagcaaaagc ggagcaagtg aagcgtcagc aagcagaagc agagcgtaaa
     3541 tcggcagagt tagcaaaaca aaaagcagaa gccgaacgcg aagcaagaga gctggcgacc
     3601 cgccaaaaag cagaacaaga acgcagcagc gccgaacttg cacgtcgaca taaaaaagaa
     3661 cgcgaagcgg cagagttgtc ggcaaaacaa aaagcagaag ccgaacgtga ggctcaagca
     3721 ttggcggtac gccgaaaagc agaagccgaa gaggcaaaac gccaagctgc agagcttgca
     3781 cgccggcatg aaaaagaacg cgaagcggca gagttgtcgg caaaacaaag ggtcggggaa
     3841 gaggaacgcc gtcaaacagc ccaatcccag ccgcaacgcc gcaaacgccg tgccgcaccg
     3901 caggattata tggcagcttc ccaagaccgt cctaaacgcc gcggacaccg ttcggtacag
     3961 caaaacaatg tggaaattgc ccaagcccaa gcagaacttg cgcgccgaca gcaggaagaa
     4021 cgcaaagcgg cagagttgtt ggcaaaacaa agggcagaag ccgaacgtga ggctcaagca
     4081 ttggctgcgc accgaaaagc agaagccgaa gaggcaaaac gccaagctgc cgaacttgca
     4141 catcgccagg aagcagaacg caaagcggca gaattgtcgg caaaccaaaa agccgcagct
     4201 gaagcccaag cattggcggc acgccaacaa aaagcccttg cgcgtcaaca ggaagaagca
     4261 cgaaaagctg cagagttagc cgtcaaacaa aaagcggaaa ccgaacgcaa aaccgccgaa
     4321 cttgccaagc aaagggcagc agccgaagca gcaaaacgcc agcaagaagc tcgccagacc
     4381 gccgaacttg cacgtcgtca agaagcggaa cgccaagcag cagagttgtc ggcaaaacaa
     4441 aaagccgaaa cagatcgaga agcggcagaa tcggcgaaac gaaaagctga ggaagaagag
     4501 caccgtcaag cagcccaatc ccagccgcaa cgccgaaaac gccgtgccgc acctcaggat
     4561 tatatggcag cttcccaaaa tcgtccgaaa cgtcgcggac gcagatctac tctgccggca
     4621 ccgccctcgc catcatttga ttcgtcagct tacgcagcac ccagggcctt gcataatccg
     4681 gactggtatg aaaatgacta tgaagaaatc cccttggatg cgttggaaga tgaagatgta
     4741 tctgaatcag ttgacacatc agacaaacag cctcaagaca atacggaact tcatgaaaaa
     4801 gttgagacag tgagtttgca accaagagcc gctcagccgc gagcccaagc cgccgcgcaa
     4861 gccgatgcag tcagcaccaa taccaactcg gctttatctg acgcaatggc aagcacgcaa
     4921 tctatcttgt tggatacagg tgcttcatta acacggcaca ttgcacaaaa atcacgcgct
     4981 gatgccgaaa aaaacagcgt ttggatgtcg aacatcggtt atggccgtga ttatgcttcc
     5041 gcacaatatc gccggtttag ttcgaaacgc acgcaaacac aaatcggcat tgaccgcagc
     5101 ttgtccgaaa atatgcagat aggcggcgta ttgacttact ctgacagtca gcatactttt
     5161 gatcaggcga gcggcaaaaa tacttttgtg caagccaacc tttatggtaa gtattattta
     5221 aatgatgctt ggtatgtggc cggcgatatt ggtgcaggca gcttgagaag ccggttacaa
     5281 acgcagcaaa aagcaaactt taaccgaaca agcatccaaa ccggccttac tttgggcaat
     5341 acactgaaaa tcaatcaatt cgagattgtc cccagtgcgg ggatccgtta cagccgcctg
     5401 tcatctgcag attacaagtt gggtaacgac agtgttaaag taagttctat gtcagtgaaa
     5461 acactgacgg ccggactgga ttttgcttat cggtttaaag tcggcaacct taccgtaaaa
     5521 cccttgttat ctgcagctta ctttgccaat tatggcaaag gcggcgtgaa tgtgggcggt
     5581 aattccttcg tctataaagc agataatcaa cagcaatatt cagcaggtgc cgcgttactg
     5641 taccgtaatg ttacattaaa cgtaaatggc agtattacaa aaggaaaaca attggaaaaa
     5701 caaaaatccg gacaaattaa aatacagatt cgtttctaaa atactaaatt catagcaaaa
     5761 taaaatgccg tctgaactca ggcttc
//
LOCUS       DAXHRI010000132         5659 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXHRI010000132 DAXHRI010000000
VERSION     DAXHRI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.132"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(129..890)
                     /locus_tag="ACFM75_001695"
     CDS             complement(129..890)
                     /locus_tag="ACFM75_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256205.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            989..1240
                     /locus_tag="ACFM75_001696"
     CDS             989..1240
                     /locus_tag="ACFM75_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256206.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1292..2098
                     /locus_tag="ACFM75_001697"
     CDS             1292..2098
                     /locus_tag="ACFM75_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6256207.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2405..3928
                     /locus_tag="ACFM75_001698"
     CDS             2405..3928
                     /locus_tag="ACFM75_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC6256208.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVES"
     gene            4026..5438
                     /gene="trkA"
                     /locus_tag="ACFM75_001699"
     CDS             4026..5438
                     /gene="trkA"
                     /locus_tag="ACFM75_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC6256209.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1414 a         1700 c         1375 g         1170 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttaaatt taatccacta tattaatatt ttgcgcctgt
       61 tctatgatgc tttcaagtcg ggtgagaatg caaatgccgt ctgaaacggc tttcagacgg
      121 catggcaatc agcgtttgta ttttaactcg tacttgatgt cgttgaggat tttgcggaca
      181 tcgtgttcca acacgtcttc gactaccgca cccgcctgct cgtgcagcat ctgctggagc
      241 tgataggtaa aaaccgccat ctgcttttgc accgccgtac ggatgatgcc gttgacggta
      301 tcggtcagat gcgggcgcag gcgtttgatc agccgttcgg tcagctcctg ttcggacagg
      361 cagaacactt cgcgccggtt gacggctttc ggattcagga tattgatttg gacgggcatc
      421 aacgcctctt ccgcaccgcc ttcgccgttt tccgcaaccc cgtctgccgc cgtacccgat
      481 tctgccgcat cggcgttttc cccgccttca atctgtccgg tttcaaattc ggcactgtct
      541 tttttggcat cgaacaggat tctccgccgc gattcgatgt gtttttccga aaccgacatt
      601 tgcagggaag cctgcgcgtt gagccagttt tcctgaagga caatcatcgg gtcggtttcg
      661 acttcctcgc cgcaatcggc aacggcggca ttgtgttcct cctgccattt tttcagatac
      721 gccttcaaca cacgggctcg gctctcatcg tccagtttcg gcacaggcgc gtccgttccg
      781 gtttcagagg ggcgggacag cggcgcgtaa gtcggcactg ccttcatacg gcgcgtctga
      841 cgcaggtttt ccaaacgttt ttcccaattc ggctctttat tcgcatccat tttcggcttc
      901 cggttcttaa tctttgcaag cagacaaacc cgcgcccaaa gcgcgtttga tataatcgcg
      961 cattttaaca gattcgcgag gatacatcat gggcagcatc gaacagcgtt tggaatatct
     1021 ggaagaggcg aacgacgtgc tgcgtatgca gaaccacgtc ctgtccaccg cattcaaagc
     1081 cttaatccgc gcccttcccg ccgaaaccgc cgaaatcgcg gtcgagtcga ttcagcttgc
     1141 ttttgaggac gccttggcag aattgagcta tgaggacagc ccccataccg atttgttcca
     1201 cgacgttact tatgcgtttt tccgtgaaaa agaacgttaa ttttatgtta aactgatttt
     1261 ttaggctttt tgattaccga aaggaatttt gatgaatatg aaaaaatgga ttgccgccgc
     1321 ccttgcctgt tccgcgcttg cgctgtctgc ctgcggcggt cagggtaaag atgccgccgc
     1381 gcccgccgca aatcccgaca aagtgtaccg cgtggcttcc aacgccgagt ttgccccctt
     1441 tgaatcttta gactcgaaag gcaatgtcga aggtttcgat gtggatttga tgaacgcgat
     1501 ggcgaaggcg ggcaatttta aaatcgaatt caaacaccag ccgtgggaca gccttttccc
     1561 cgccttgaac aacggcgatg cggacgttgt gatgtcgggc gtaaccatta ccgacgaccg
     1621 caaacagtct atggacttca gcgacccgta ttttgaaatc acccaagtcg tcctcgttcc
     1681 gaaaggcaaa aaaatatctt cttccgacga tttgaaaaac atgaacaaag tcggcgtggt
     1741 aaccggctac acgggcgatt tctccgtttc caaactcttg ggcaacgaca atccgaaaat
     1801 cgcgcgcttt gaaaacgttc ccctgattat caaagaactg gaaaacggcg gcttggattc
     1861 cgtggtcagc gacagcgcag tcatcgccaa ttatgtgaaa aacaatccga ccaaagggat
     1921 ggacttcgtt accctgcccg acttcaccac cgaacactac ggcatcgcgg tacgcaaagg
     1981 cgacgaagca accgtcaaaa tgctgaacga tgcgttggaa aaagtacgcg aaagcggcga
     2041 atacgacaag atttacgcca aatattttgc aaaagaagac ggacaggccg caaaataagc
     2101 ccgcccgtcc gaacacaatg ccgtctgaag ccttttcaga cggcattgtt catcaaccgg
     2161 cctacaatga actgcctgct gatttctccc taccgcaaag caacaggcaa agattacaaa
     2221 tatcaaaatc cgagtaaaac agtattttat taaaacaaat tgataatcaa gagattagaa
     2281 ttatgtattg tctttaccgt acaaacgctg gcactatttc aacctgataa aaaacagcct
     2341 tcaaaaaggt tgcttaaaac agcagcagac acttaccgcc acaaccttga aaaggaacac
     2401 aatcatgacc gtcatcaagc aagaagactt tatccaaagt atctgcgatg ccttccaatt
     2461 catcagctac taccatccga aagactacat cgacgcgctt tataaggcgt ggcagaagga
     2521 agaaaatccc gccgccaaag acgcgatgac gcagattttg gtcaacagcc gtatgtgtgc
     2581 ggaaaacaac cgccccatct gccaagatac cggtatcgca accgtgtttt tgaaagtcgg
     2641 tatggatgtg cagtgggatg cggatatgag cgtggaagaa atggttaacg aaggcgtgcg
     2701 ccgcgcctac acttgggaag gcaatacgct gcgcgcttcc gttctcgccg accccgccgg
     2761 caaacgccaa aacaccaaag acaacacccc cgccgtcatc catatgagca tcgtgccggg
     2821 cggtaaagtc gaagtaacct gcgcggcaaa aggcggcggc tctgaaaaca aatccaaact
     2881 cgccatgctc aatccttccg acaacatcgt cgattgggta ttgaaaacca tcccgacgat
     2941 gggcgcgggc tggtgtcctc ccggcatctt gggcatcggc atcggcggca cgcctgaaaa
     3001 agccgtgttg atggcaaaag aatccctgat gagccacatc gacattcaag agttgcagga
     3061 aaaggccgcg tccggcgcag aattgtccac caccgaagcc ctgcgcctcg aactctttga
     3121 aaaagtcaac gcgctgggca tcggcgcaca aggtttgggc ggtctgacca ccgtgttgga
     3181 cgtgaaaatc ctcgattacc cgacccacgc cgcctccaaa ccgattgcca tgattccgaa
     3241 ctgcgccgcc acccgccacg tcgaatttga attggacggc tcaggccctg tcgaactcac
     3301 gccgccgcgc gtcgaagact ggcccgattt gacttacagc cccgacaacg gcaaacgcgt
     3361 cgatgtcgat aagctgacca aagaagaagt ggcaagctgg aaaaccggcg acgtattgct
     3421 gctgaacggt aaaatcctca ccggccgcga tgccgcacac aaacgcctcg tcgatatgct
     3481 caacaaaggc gaagaattgc ccgtcgattt caccaaccgc ctgatttact acgtcggtcc
     3541 cgtcgatccg gtcggcgatg aagtcgtcgg tccggcaggt ccgaccacag ccacccgcat
     3601 ggacaaattc acccgccaaa tgctcgaaca aaccgacctc ttgggcatga tcggcaaatc
     3661 cgagcgcggc gtggccacct gcgaagccat cgccgacaac aaagccgtgt acctcatggc
     3721 agtcggcggc gcggcgtatc tcgtggcaaa agccatcaaa tcttccaaag tcttggcgtt
     3781 ccccgaattg ggcatggaag ccatttacga atttgaagtc aaagacatgc ccgtaaccgt
     3841 cgccgtagac agcaaaggcg aatccatcca cgccaccgcc ccgccccaat ggcaggcgaa
     3901 aatcggcatc atccccgtcg aatcttgagg cgtcatgccg tctgaacaca aaatctgctt
     3961 tcagacggca tttccgcccc cggttgcggt acaatccacc atttcatcac tcggcgaccc
     4021 acaccgtgaa aatcctcatt ttaggcaacg gacaggtagg ttctaccgtc gcacaaaacc
     4081 ttgccgccat acccaacaac gacgtaaccg ttatcgacat cgacgaaaaa gcattgcagg
     4141 aaacaggcag ccgcctcgat gtccaaaccg ttttcggcaa cggcgcatcc cccttcacat
     4201 tagaacgcgc cggcgcggaa gatgccgact tgctgctcgc gctctcccgc agcgacgaaa
     4261 ccaacatcgt cgcctgcaaa gttgccgccg acctgttcaa catccccggc cgcatcgcgc
     4321 gcgtccgttc cagcgaatac ctcgaatacc tcagccccaa gctcgaaaac aacgaaaacg
     4381 gcagcctttc catattcggc ataaccgaaa ccatcagccc cgaacagctc gttaccgaac
     4441 agcttgccgg cctgatagac tgcccgggcg cattgcaggt tttacgtttt gcagacgacc
     4501 gcgtgcggat ggtcatcata caggcgcggc gcggcgggct gcttgtcgga cgcagcattg
     4561 ccgacatcgc ccaagatttg cccgacgggg ccgactgcca aatctgcgcc gtttaccgca
     4621 acaaccgcct catcgtcccc gcgccgcaaa ccgtcatcat cgaaggcgac gaaatcctgt
     4681 ttgccgccgc cgccgaaaac atcggcgcgg tcatacccga attgcgcccc aaagaaacca
     4741 gcacccgccg catcatgatt gccggcggcg gcaacatcgg ctaccgtctc gccaagcagc
     4801 tcgaacacgc atacaacgtc aaaatcatcg aatgccggcc gcgccgtgcc gaatggatag
     4861 ccgaaaacct cgacaacacc ctcgtcctgc aaggttcggc aaccgacgaa accctgctcg
     4921 acaacgaata catcgacgaa atcgacgtat tctgcgccct gaccaacgac gacgaaagca
     4981 acattatgtc cgcccttttg gcgaaaaacc tcggcgcgaa gcgcgtcatc ggcatcgtca
     5041 accgctcaag ctacgtcgat ttgctcgaag gcaacaaaat cgacatcgtc gtctcccccc
     5101 acctcatcac catcggctcg atactcgccc acatccggcg cggcgacatc gttgccgtcc
     5161 accccatccg gcgcggcacg gcggaagcca tcgaagtcgt cgcacacggc gacaaaaaaa
     5221 cttccgccat catcggcagg cgcatcagcg gcatcaaatg gcccgaaggc tgccacattg
     5281 ccgccgtcgt ccgcgccgga accggcgaaa ccattatggg acaccatacc gaaaccgtca
     5341 tccaagacgg cgatcacatc atctttttcg tctcgcgccg gcgcatcctg aacgaactgg
     5401 aaaaactcat ccaagtcaaa atgggctttt tcggataaac cgccccattc cggacatatt
     5461 gccgccaagc ggtatggaag cggaataatg gtaggtgggc ttcagacggc atccgccctc
     5521 cccgtcattc ccgcgtaagc gggaatccag accttgggat agcggcaata ttcaaaggtt
     5581 agctgaagct ttagagattc tagattcccg tttccacggg aatgacgaaa ggttgcggga
     5641 atccaaacca ttgggcaac
//
LOCUS       DAXHRI010000133         5651 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXHRI010000133 DAXHRI010000000
VERSION     DAXHRI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.133"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..588
                     /locus_tag="ACFM75_001700"
     CDS             <1..588
                     /locus_tag="ACFM75_001700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256210.1"
                     /translation="IKRQEQKGELISDFYRSNITKKAIAERWELDILAKQQEKAKIEK
                     AERERKKAEEQAEQARKEKEKQKLEEQRKFLIEQFEVLSPKQQENVINEIFEQVGKGT
                     FKQIFQTARQQGTVHIDARFVKNFYNYFQQEERAKRARKRVDLPIQYTEEDIEQEVPK
                     EKGTIFFGNDWNEYVYKQLIMYIDFKSMMNDDAIK"
     gene            785..1934
                     /locus_tag="ACFM75_001701"
                     /pseudo
     CDS             785..1934
                     /locus_tag="ACFM75_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247816.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            2164..2499
                     /locus_tag="ACFM75_001702"
     CDS             2164..2499
                     /locus_tag="ACFM75_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6256211.1"
                     /translation="MKELNISDLEIVSGGSEPKYFSSLDVIGVTSAATGGWRAVSATA
                     TGDMIARSAARGGLIGLAGIAGWNTGTYLYHETPLGDWLSSGVDKLTGLDQNFNDASG
                     NDYGDGTGY"
     gene            3091..3426
                     /locus_tag="ACFM75_001703"
     CDS             3091..3426
                     /locus_tag="ACFM75_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256212.1"
                     /translation="MYELSIVELELVSGAGPIADFTTGVGGAIGGGAGYIAGKAGGGT
                     IGGILGSPFGPGGTIVGVAAGQQYGGKVGSVIGAGAGSAIGGVIGRGIESILDNDRSG
                     NDYGDGTGY"
     gene            3643..4724
                     /locus_tag="ACFM75_001704"
                     /pseudo
     CDS             3643..4724
                     /locus_tag="ACFM75_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214481.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
     repeat_region   5059..5622
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5125..5160
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
BASE COUNT         1756 a          883 c         1155 g         1857 t
ORIGIN      
        1 atcaaacggc aagaacagaa aggcgagcta attagcgact tctaccgttc caacatcacg
       61 aaaaaagcca ttgccgaacg ttgggagctg gatattttgg ctaaacagca agagaaagcc
      121 aaaattgaaa aagcggaacg tgagcggaaa aaagccgaag aacaggcaga acaagctaga
      181 aaagaaaaag aaaagcaaaa gcttgaagaa caaagaaaat ttttgattga gcagtttgaa
      241 gtattgtctc ccaaacagca agaaaatgtc atcaatgaga tatttgagca agtgggaaaa
      301 ggcacattca agcaaatttt ccaaacggca cggcagcaag gtacggttca tattgatgcg
      361 cgatttgtaa aaaattttta taattatttt caacaagaag aacgcgcaaa aagggctaga
      421 aaacgtgtag atttacccat tcagtacaca gaagaagata ttgagcagga agtaccaaaa
      481 gaaaaaggca caattttttt cggtaacgac tggaacgaat atgtttataa acaactgatt
      541 atgtatatag attttaaaag catgatgaat gatgatgcca ttaaataaat ggaacttgtg
      601 atgagaatgt ttaaattaac ggccgatgac ttggaaaaac tttaatatct tgaaaaattt
      661 taggagagta attgtggctt aaatttgtgg tacttttcaa aaaaattaaa catacttaac
      721 gaaatttgac gttctaatat tttattttta tactcaaaat cattattaat aacaaggatt
      781 atttgtgctt attaactttt caatggaacg caaatatttc catgaaaatt atttatataa
      841 aaagccattt atttttaaaa aatctttaga tgtaagttgt atttcgtgga aagaaatcaa
      901 tgaattgtat caacgtgcag accctactga ttggcaattt aagttccgaa aaggggaaat
      961 aattccaaaa gaagcctatg ttgaatcatt caatgatgtg ggtaggatac gtcatcgatt
     1021 taataaaaca gccgtgtatc agtatttgca agatggcgcg acaatggttt ataaccgtat
     1081 tgataatgag ccatttgttg atagtattgc taaacaaatt gctcagtttg ctcaagcata
     1141 aactgttgtt agtggttatt tagcttttgg tagctcatca tcatatcgta atcattggga
     1201 tactcgagat gtatttgcag tacaattgat tggcaaaaaa cattggacaa tttctgcccc
     1261 aaattttgat atgccgttgt atatgcagca agcaaaagat atgccgcaca ttactccacc
     1321 taaaacagtg gatatggaag tcattttaga agcaggagat attctatata ttccacgtgg
     1381 ttggtggcat aatcctatgc caatgaattg tgaaacattc catttagcta ttggtacatt
     1441 tccaccaaac ggttataact atatggaatg gttgatgaaa aaaatacctg atattcagag
     1501 tattcgtcaa aatttcatag gctgggaaca tgaccaaaaa aaatctagat gattcagctc
     1561 aagcagttac tgaaatgatg aataacccaa aaaattatca agcatttatg caagattttt
     1621 tgggaaatca gcgcaccaat acagcattca atatggattt gtttggaaat gctcataatc
     1681 agacattacc tgagcattgc tttttaagat taaatagtaa tgattgctca actctttcac
     1741 aaggatactt tattgcgaat ggcattaaag taaatattga tgaaatcagt atgaaatttt
     1801 taacgatttt agttaataaa cacataatcc ccttaactga aatgttatcg ttatttaata
     1861 ctaacgaaca agaaagtatt aataaattag tttggcagct aggagagtta gatataattg
     1921 aaattataag atgagatgga tttaatattt taggatataa aatatatgaa aacattttta
     1981 catgcaattg gatattctgt tgtaatatat atttttatga aattatcaga attaggaggg
     2041 tggcgtcttc atcctaatta tgacggagat tacattaata tagtatttag tattatgatt
     2101 ggtattggca tgagcgtact attcaatcat attgaaaaat aagattgtta aaaagggaaa
     2161 gcaatgaaag aattaaatat ttctgacctg gaaatagttt caggtggttc ggaaccaaag
     2221 tattttagtt ctcttgatgt tattggtgta actagtgcag cgacaggtgg atggagagct
     2281 gtttcagcta ctgcaactgg ggatatgata gcaagaagtg ctgctcgagg tggattaatt
     2341 ggtttggcag gcatagcagg ctggaataca ggtacttatt tatatcatga aacgccatta
     2401 ggcgattggt taagttcagg agttgataaa ttaacaggac tagaccaaaa ttttaatgat
     2461 gcatctggta atgattatgg cgatggaaca gggtattaaa caaaattata ggaatagcat
     2521 tgatgaatgc ttaccaagta ctcatggctt tctttcttgc tttacatgat atccctagct
     2581 gaatattatc gttgtttagt gttgatgaac aagaaaatat taagaaatta gttagggagt
     2641 tatgtttatt agatgttatt gaaatcgtga ggtaatcaat gggtgtttta ttaaataaca
     2701 caaaaatttt aatcaatata tcctcaatat catttatttt attgaacgta tacattatat
     2761 acagtaaagt tagatttaat ttatcaacta taccttttgt aaatcatatt tctatattaa
     2821 tagcattttc gtttataatt tcaacattgc ttttgattta taaaatggta atttttgcac
     2881 gaggaataaa taaaaatcaa aataaaatag aatttgtaaa aatatttact ttttatttta
     2941 tggttatttt gtatattgcc ttaataactc tatttagaca ggaaaatttt tattttaata
     3001 tgaatattta ttctatcatt tttatagtga tactattctg tctaactcat ttctttttta
     3061 ttattaaatt ttattttgag gtaaataaaa atgtatgaac tctctattgt agaacttgaa
     3121 ttagttagtg gtgctggtcc cattgctgat ttcactacag gagtgggagg tgctattggt
     3181 ggtggtgctg gatatattgc agggaaagca ggtggtggaa ccatcggggg aattctaggc
     3241 tctccatttg gtccaggtgg tactatagtt ggtgttgcag ctggacaaca atatggtggt
     3301 aaggttggtt ctgtaattgg ggcaggagct ggatctgcca ttggtggtgt gatcggacga
     3361 ggtattgaaa gcattttgga taatgatcga tcaggtaatg attacggtga tggaacaggg
     3421 tattaaacaa aattatagga atagcattga tgaatcctta ccaagtactc atggctttct
     3481 ttcttgtttt gatttttttg gttggcttat ctgtacatta cggattaata catcagcctc
     3541 cattaagtcg taatcaaatg aaaatagttt tattttttta ttatcagtat tattatcggt
     3601 aataataatt ttttttaaat aaaaaataaa ataggaactc taatgccaac atatctctct
     3661 cgcctatcat tcagtttctc gcgccgtttg cccgttattt tgcaaacgga agcagccgaa
     3721 tgtggattag cgtgtttggt atcggtgttg gggtttcatg gtttttatac ggatttgcgc
     3781 catttaaggg cgcgtttttc tttgtcgctt aaaggcgcaa cattggcgga tttggtacgg
     3841 tttgctaata gcatgaattt aaccgctcgg gctgttcggt tggatttaga tgagttagca
     3901 aatttgcgcg taccctgtat tttgcattgg gatttaaatc attttgtggt gttgcatgaa
     3961 gttcatcgca atcacattat gattatgaac cctgcttcgg gttggcaaaa agtgcgtatg
     4021 gaagaagtct cgcgctgttt tacaggtgta gcattggaac tatttcctaa cacgcaattt
     4081 gaagagaaac acgaaacgcg taaaattcgt gtactaccga tgttgcgcgg agtggtggga
     4141 ttaaaaagat ctttatttca gcttttaccg ttggctactg tattacaggt atttgctttg
     4201 gcaagccctt tttttatgca atgggtaata gaccatgcca ttgtatcggc agaccgtgat
     4261 ttattgctga cattggcatt gggttttggc ttgttgatga ttgtgcagca gttggtatca
     4321 ttgttacaaa cttgggcggg tatgtactta tctacctctt tgaatattca atggaaagct
     4381 aatgtattac gccgcttaat ggatttgttg gtgcaatttc tgtgttggac ggtgttttta
     4441 ccgttggggc gttaatggcg tttatgtcct acaaaagtca gtttgagagt aaagcagggt
     4501 cattgattga ccagtttgtg caaatcaaaa tgctaggatt gcacgctgaa cgtttggcgg
     4561 atattgtgct agaagaaaca gaaaacgagc agaacaatga tttcaggctg cctgaaaatt
     4621 tagaacactt tgatattcaa atagaaaatg tttcttttcg ctatgctgaa aatgaaccat
     4681 acattttgca agattttagt ttaatcatca aacagggaac agcaaaaatc cccgctgcaa
     4741 cggggatttt tgttcggctc tcgcctgtgt gattaacttc actaggagaa gctaaaatgc
     4801 ttaaattcct tatgcgtggg aaaaagttgc tgctgtccgt tgaagtgaac cacaagttca
     4861 ttttagcgat tgctttgctg ttaagtcaat gcagtagcta acccgaccac aatcaaaaaa
     4921 acgcaagttt tccgcctact ctgtgagtgg gcggtttttt cttatttcaa atctgtgaca
     4981 caatatctgt cgcctttctt gccataattg cactgtgtct caatgacttt aacgcacgtt
     5041 cgcttatcgc aacggctggt tgtagcgccc attctcattt cgcagtgcta caatttctat
     5101 tataaaagct gcctgactta ctcggttgta gctccctttc tcatttcgca gtgctacaat
     5161 ggcggatggc atacggcgtg ccttttcggt gttgtagctc cctttctcat ttcgcagtgc
     5221 tacaatgagc attgacacta tttgtatggc gttaaggttg tagctccctt tctcatttcg
     5281 cagtgctaca atccgtcact acacgaactg gcggacttct tggttgtagc tccctttctc
     5341 atttcgcagt gctacaatag catttgggcg gcacgggcaa agccgtcagt tgtagctccc
     5401 tttctcattt cgcagtgcta caataatcag accgacttta caacgaaggt ttgagttgta
     5461 gctccctttc tcatttcgca gtgctacaat cgacaacaaa gccgtctatg acctgaccga
     5521 gttgtagctc cctttctcat ttcgcagtgc tacaatttgg gaggtggaaa accgaatcct
     5581 gcacgagttg tagctccctt tctcatttcg cagtgctaca ataagcaaat cattttcata
     5641 gtcagcagct t
//
LOCUS       DAXHRI010000134         5551 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXHRI010000134 DAXHRI010000000
VERSION     DAXHRI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.134"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..86)
                     /locus_tag="ACFM75_001705"
     CDS             complement(<1..86)
                     /locus_tag="ACFM75_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6256213.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPV"
     gene            complement(472..747)
                     /locus_tag="ACFM75_001706"
     CDS             complement(472..747)
                     /locus_tag="ACFM75_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6256214.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(744..2027)
                     /locus_tag="ACFM75_001707"
     CDS             complement(744..2027)
                     /locus_tag="ACFM75_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6256215.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2183..3601)
                     /gene="glnA"
                     /locus_tag="ACFM75_001708"
     CDS             complement(2183..3601)
                     /gene="glnA"
                     /locus_tag="ACFM75_001708"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC6256216.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3685..3834
                     /locus_tag="ACFM75_001709"
     CDS             3685..3834
                     /locus_tag="ACFM75_001709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256217.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLRFVCSKAEYPPFCPNAD
                     KTDAA"
     gene            3912..4721
                     /gene="aroE"
                     /locus_tag="ACFM75_001710"
     CDS             3912..4721
                     /gene="aroE"
                     /locus_tag="ACFM75_001710"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC6256218.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSREIFRNCRLAYDMVYGDA
                     AQAFLNFAQSNGAAEVSDGLGMLVGQAAASYHIWRGFTPDIRPIIEYMKAL"
     gene            4724..5425
                     /gene="mtgA"
                     /locus_tag="ACFM75_001711"
     CDS             4724..5425
                     /gene="mtgA"
                     /locus_tag="ACFM75_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC6256219.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTACVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
BASE COUNT         1359 a         1520 c         1424 g         1248 t
ORIGIN      
        1 ttaacaggtt cacatgaggt catagtaaaa acactcccaa taaacaaaat aaaaagttga
       61 taaatggtag tagcatatgg tctcataatt tcaagcttag aaattagtta aagaatatat
      121 tatggtagat actaactctt taagttgaca tattgtgcga tatcgagggc tgtaccgtat
      181 tttcccatcg tattttcccg ttctttatat gagcggcagg catcctcata aaagaaggga
      241 taccgataag gaaaaatacc ccagcataaa aaatatactg attaatcaat acaaatccaa
      301 caaacagttc atcgtattct tttctgttgg cgtataaaaa atactgatat atgccttcac
      361 tttcataggg cgacgcttta tataccgtcc tgtgtgttga aacgtcggtt ttccttaccg
      421 cctgccccgc ttttccctga ttgctgccaa ctgccgtccg aacctgaacc gtcaggtttc
      481 agacggcatt ttttccgcct gttcccacaa ttgccgtttt tgtgcggcgg tcagtttttt
      541 gacggcgatt tcctgcctga gtgccgtgcc tttatccatc ccgcctgcaa cgatacgcat
      601 cgccaccggt ttgaatacgc gggtatattt cgcgccttta ccggttgtgt gggcggcaag
      661 ccgctgttgc ggattcgggc tgatgccgca atagaacgcg ctgttttcac acaatatcag
      721 gtaaacgctc cagtttgacg cgttcatctg cctgcatcct gagttttctc cccgttccaa
      781 ggcgcaactt tcagcagcag cagcataccc ggtagggcga gtgcgaaaca cagttggaaa
      841 aacggtacat aaccgagcca ttcaatcaga taaccggcaa aggaattgat gaccgtgcgc
      901 gggacggcgg acaggctggt aaacagcgca agctgcgttg cggtaaatgc gggattggtc
      961 tcgcgcgcca tatacgatac gaacgccgcc gtccccaaac ccacgccgac tgcctctgcg
     1021 ccgataactg ccgccagcat cagcctctcg cctgtgccga ccgtgtcgaa atgtccgaac
     1081 cctgccagcc atacaaaccc caaaacggtt acagcctgca ccgcgccaaa tagccacaag
     1141 gctttgttta cgccgatttt cagcatccac acgccgccca agatacctgc cgccactgcc
     1201 ggccacagtc ctgcattttt cgcaatcaaa ccgatgtctg tcttgctgaa acccatatcc
     1261 agataaaacg gcgttgccaa cgcggttgcc atactgtcgc cgagtttgta aaggaagata
     1321 aacagcagca cgcacaccgc cgaagcgatg cccttgcgcg taaaaaattc tttaaacggc
     1381 tctaccacgg tctgcttcaa cgttttagga acggcaggcg gcaacacggg ttcgcgcgca
     1441 agaaacagcg tcatcagaag gccgggcagc ataaataatg aagtgataac aaatacttct
     1501 gaccacggca tcctgtctgc caacaccaaa ctcaatgaac cgggaatcag ggcggcaatc
     1561 cggtaggcgt tcacatgaac cgagttgccc aaacccaatt cttcgtctga caaaatctcg
     1621 cgcctgaacg catccaatac aatatcctga ctggcggaaa aaaaagcgac aagcaccgac
     1681 aagccggcaa tcagcggcag atgattacgg gggtttaaaa aggcataggc cgccaaagcc
     1741 gccagcaacc ctgcctgcgt cagcagcatc cacccgcgcc gccgtcccaa aacgggcagc
     1801 ctgaccgcgt ccatcagcgg cgaccacaaa aatttccaag taaacggcag accgattaac
     1861 gccatcagcc cgatgctctt caaatccacc tgctcgctgc gcaaccacgc cggaatcagg
     1921 ttaatcagaa agtacagcgg cagccccgag gtaaaccccg taaaaataca gacaagcata
     1981 tttcgggaaa agatttgccc gataaaacct gatttcgatg cagtcatcgt tcgtgcagcc
     2041 caaaaaaaac aaaagcctat tgtagcaaat atcgcccgcc tgctgaaaaa tgccgccccg
     2101 cgccgccggt ttgccgttca aatgcaaaat gccgtctgaa actgtgttca gacggcattg
     2161 cttttctgaa aactagacgt gcttacaggc tgtaatacat ttcaaattcc agcgggtgcg
     2221 gcgccatgcg gatgcggcgt acgtcttctt ctttgaaagc gatgtagctg tcgatccagt
     2281 ctttgctgaa cacgccgccg cgcaggagga attcgtggtc ggctttgagg gcggccagtg
     2341 cttcttccaa agaagcgcaa acggtcggca ccaatgcatc ttcttccggc ggcagatcgt
     2401 acaggttttt atcggcaggg tcgcccggat ggattttgtt ttgaatgccg tccaaacccg
     2461 ccatcaacag ggcggcaaat gccaaatacg ggttggcggt cggatcgggg aaacgcgctt
     2521 cgatgcggcg cgccttgctg ctgttcacgg acggaatgcg gatggaagcg gaacggtttt
     2581 tggcggagta tgccagtttg gtcggcgctt caaagtgcgg cacgaggcgt ttgtaggagt
     2641 tggtggacgg attggtaatc gcgttcaagg ctttggcgtg tttgatgatg ccgccgatgt
     2701 agtagagcgc ggtgtcgctc aagccggcat agccgtcgcc tgcgaacagg ttttgaccgt
     2761 ctttccaaat ggattggtga acgtgcatac cgctgccgtt gtcgcccata atcggtttgg
     2821 gcataaaggt ggctgttttg ccgaagttgt gggcaacgtt ttgaatcaca tatttcatgt
     2881 cttgggtttg gtcggcgcgt ttgaccaagg tggcgaaacg cgtgccgatt tccatttggc
     2941 tgccggtacc gacttcgctg tggtggactt cgacttcgat gccgagttct tccaaaatgt
     3001 tcaccatggc ggagcgcaaa tcttggccgc aatcaatcgg cgcgacgggc gcgtaaccgc
     3061 ctttaacggc ggggcggtgg ccggtgtttt gaccgtccat atgcagaccg cttgcccatg
     3121 cgccgctttc ggacgtgatt tcgtaacggg ttttgtgcat atcggtttca aattctacgc
     3181 cgtcgaagac gaaaaactcg ggttcggggc cgaagtatgc cgtgtcgccg ataccggaag
     3241 atttcaaata ggcttcggcg cggcgtgcga tggagcgcgg gtcgcggtcg taaccctgac
     3301 cgtcggcagg gtcgatgacg tcgcaggtaa tgacgacggt aacatcatca taaaaaggat
     3361 caacgaaggc tgtagacgca tcgggacgca gctgcatatc ggaagcctga atgcctttcc
     3421 agccgccgat agacgaaccg tcaaacgcct gaccgttttc gaaccactct tcggggtctt
     3481 ccaacacgat gcgcgcaggc acggtaaagt ggtgctgctt gcctttggta tcggtaaagc
     3541 gcaaatcgac aaagcgggct tcgctttctt caatcaattt tacggcgttt ttaatagaca
     3601 tttttcagct cctggaaaag ggaaatagca aaatatacgg aacgcggcag attagcccaa
     3661 agccagcatg attctaccac gtccatgccg tctgaaactc gccctgcgtc aaatttcaag
     3721 ctggaaaata tagtttcaga cggcaagatt gtcaacaata tgcttcggtt tgtctgttca
     3781 aaagcagaat acccgccctt ttgtccgaat gccgacaaaa cagatgccgc ctgaaaacag
     3841 tttagacggc atcgttccca tcttcgcgcc gccgttataa aatgaccgcc ccgccttgat
     3901 ggaatatgac tatgcacgcc ctcccccgct acgccgtttt tggcaacccc gtcgcccaca
     3961 gcaagtcgcc gcagattcat caacaatttg cccttcagga aggcgttgac attgaatacg
     4021 aacgcatttg cgccgacatc ggcggtttcg cgcaggcggt ttcgacattt tttgaaacag
     4081 gcggttgcgg ggcaaacgtt accgtaccgt tcaagcagga agcgtttcat ctggcggacg
     4141 agcattctga acgcgcattg gctgccggcg cggtcaacac gctgattttt ctgaaaaacg
     4201 gaaaactgcg cggcgacaat accgacggta tcggtttggc caacgacatc acgcaggtca
     4261 aaaacattgc catcgaaggc aaaaccatct tgcttttggg cgcgggcggc gcggtgcgcg
     4321 gcgtgattcc tgttttgaaa gaacaccgtc ctgcccgtat cgtcattgcc aaccgcaccc
     4381 acgccaaagc cgaagaattg gcgcggcttt tcggcattga agccgtcccg atggcggatg
     4441 tgaacggcgg ttttgatatc atcatcaacg gcacgtccgg cggcttgagc ggtcagcttc
     4501 ctgccgtcag ccgtgaaatt tttcgcaact gccgccttgc ctacgatatg gtttacggcg
     4561 acgcggcgca ggcgtttttg aactttgccc aaagcaacgg cgcggccgaa gtttcagacg
     4621 gcctgggcat gctggtcggt caggctgccg cctcctacca tatttggcgc gggtttacgc
     4681 ctgatatccg ccccattatc gaatacatga aagccctgta accatgttcc gcatcatcaa
     4741 atggctgatt gccctgcccg tcggcatctt tatctttttc aatgcctatg tgtacggcaa
     4801 catcattacc taccgcgccg tcgcgcccca tcagactgcc tttatgtcga tgcggatgaa
     4861 gcagtttgaa caggaaggtc gcgatgtcgc actggattac cgctggatgc cctacaaccg
     4921 catctcggtc aacctgaaaa aagccctgat tgcttccgaa gatgcccgtt tcgccgggca
     4981 cggcggcttc gattggggcg gcattcaaaa cgccatcagg cgcaaccgga acagcggcaa
     5041 agtgaaggcg ggcggctcga ccatcagcca gcagcttgcc aaaaacctct tcctcaacga
     5101 aagccgcagc tatatccgca aaggcgaaga agcggcgatt accgcgatga tggaagccgt
     5161 taccgacaaa gacaggattt ttgaactgta tttaaactca atcgaatggc actacggcgt
     5221 tttcggcgcg gaagccgcgt cccggtattt ttatcaaata cccgccgcca agctgaccaa
     5281 acagcaggcg gcaaaactga cggcgtgcgt ccccgccccg ctctactacg ccgaccatcc
     5341 gaaaagcaaa cggctccgca acaaaaccaa tatcgtgctc agacgcatgg gttcggcaga
     5401 gttgcctgaa agcgacacgg actgattgtt tcagatatgg aaatgccgtc tgaactgcgg
     5461 ttcggacggc atatgttttc tggtttttat agtggattaa caaaaatcag gacaaggcga
     5521 cgaagccgca gacagtacaa atagcacgga a
//
LOCUS       DAXHRI010000135         5507 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXHRI010000135 DAXHRI010000000
VERSION     DAXHRI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.135"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(254..2050)
                     /locus_tag="ACFM75_001712"
     CDS             complement(254..2050)
                     /locus_tag="ACFM75_001712"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC6256220.1"
                     /translation="MNTVSNYLSALREAMRAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPNDLLDQVWTSRPALPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPIFVSFLL
                     IGKDNAVLFTEQCRLNAEAAAALQTAGIAVEPYAQVAGKLAQIGGALLIEPNKTAVST
                     LVRLPKSVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2106..2297
                     /locus_tag="ACFM75_001713"
     CDS             2106..2297
                     /locus_tag="ACFM75_001713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256221.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFMPFFGSNTENIVKFEH"
     gene            2508..3761
                     /locus_tag="ACFM75_001714"
     CDS             2508..3761
                     /locus_tag="ACFM75_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6256222.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3767..3919
                     /locus_tag="ACFM75_001715"
     CDS             3767..3919
                     /locus_tag="ACFM75_001715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256223.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            3916..5427
                     /gene="lysS"
                     /locus_tag="ACFM75_001716"
     CDS             3916..5427
                     /gene="lysS"
                     /locus_tag="ACFM75_001716"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC6256224.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEILDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVNERYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTAKISYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
BASE COUNT         1189 a         1402 c         1626 g         1290 t
ORIGIN      
        1 cggaatatag cggattaaca aaaaccagta cggcgttgtc gcgccttagc tcaaagagaa
       61 cgattctctc aggtgctgaa gcaccgagtg aatcggttcc gtactattcg tactgtctgc
      121 ggcttcgtcg ccttgtcctg atttttgtta atccactata tttgcttgcg gcaagatgaa
      181 aacggtgcgg gatgttttgg gaaaccaatg ccgtctgaag ggctttcaga cggcattttt
      241 tgcgccgtgc tgtttaacgc gccagcggtt cggtgcgttt gatcagccac gcttttgccg
      301 cgccttcggt cagcggctcg aggcggcggc ggacttcggc gtggtagcgg ttgacccagt
      361 cgatttcgcc gtcggtcatg agggcggtgt ccatcaggcg ggtgtcgatg gggcagaggg
      421 tcagggtttc aaaacagagg aagctgccga attcggtttc ttgaggggcg gcgacggctt
      481 ggttggcggc aaggttttca atgcggatgc cccattttcc cgggcggtag agtccgggtt
      541 cgatggaggt aaccatgcct tttttcatgg cggtttcggg cgtggcgggg gcggcgaagg
      601 cgatgcgctg cgggccttcg tggacgttga ggaaatagcc tacgccgtgt ccggtgccgt
      661 ggccgtagtc gcattgcgcc tgccacaggg gtttgcggca aatcgcatca atcagcggcg
      721 aggggatgtt ttctgggaac acggcttcgg caagcgcgat atgggctttg agaacgaggg
      781 tgttgtcgcg tttttgttcg gcggtgggtg tgccgacagg gacgacgcgg gtgatgtcgg
      841 tcgtgccgcc tttgtattgc gcgccggagt cgatgagcaa aagcccgttg ccgctgatgg
      901 tgctgtggct ttcgggcgtt gcgctgtaat gcggcagtgc gccgttggcg ttgaagcctg
      961 cgatggtgtc gaaactcaat gaaatgaagc ctgggcgcac gctgcggtgg cgataaagca
     1021 tggtgtccac gtcgatttcg gtcaggctgc cgccgttgcc gatgatgtct tcaaactcgg
     1081 cgaagaaacc gcacaacgcc gcgccgtcgt gttccatcgc ttcgcggatg cgggcgatgt
     1141 cggcttcgga tttgcaggat ttgaacagcg tggacgggtt gatgccttca atcaggcgca
     1201 cgcttttggg caggcgcaca agggtgctga cggcggtttt gttcggttcg ataagcaacg
     1261 cgccgccgat ttgcgcgagt ttgccggcaa cttgggcgta aggctcgacc gcgatgccgg
     1321 cggtttgcag cgcggcggcg gcttcggcgt tcagacggca ttgttcggta aacaggacgg
     1381 cgttgtcttt gccaatcagc aggaaggaga cgaaaatggg attgaaaggc acgtcgctgc
     1441 cgcgcaggtt ggtcagccag gcgatgtcgt caagcgagga aaccaagtgg taatccgcgc
     1501 ctttttccgc catgacggcg cgcacgcggg cgagtttttc ggcggcggtt tcggagacgt
     1561 aggcggggtc gtggatgaac acggtttcgg cggggagggc gggacggctt gtccacactt
     1621 ggtcaagcag atcgtttggg tgttcgatgc ggatgttttt ggcggtcagg gattgcgcca
     1681 aagtgcgttt gccggtgagc gagaccatat cggaagggat gccgacggcg gcgttttcgg
     1741 gcaggtttgc cgcgagccat tcgttatacg gcggcacttg cccgcttttt tgcagctcaa
     1801 tgccgctgcc ggaaagctgt ttggcggctt gttcccaata gcggctgtcc acccacacgc
     1861 ccgcttcgtc ggcggtaacg acaaatgtgc cgaccgagcc ggtaaagccc gacaattcgc
     1921 ggcgcgcctg ccagtgtgcg ggcaggtatt cggacaggtg ggggtcggcg gaagggatga
     1981 cgagtgcgtc caagccttgc gccctcatgg cttcgcgcaa tgcggacagg taattcgata
     2041 cggtattcat aacagttcct ccaatcgggt tttgcggctt cagacggcat ggacggaaat
     2101 ctgaaatgcc gtctgaaaaa cataaccatc ttatcaaatc gccagcctgc tgcaaaacgg
     2161 acgggctgat cggatggtgc gtgtttgcgc gaaaaaagcg atgtaaggtt gtttgttgta
     2221 aaattttagg cataaaatgt cgtttcatgc ctttttttgg aagtaatacg gaaaatatag
     2281 tcaagtttga gcattaagtt ctaatagtgt gtgttggcag gtatttggat tacgttacaa
     2341 tacgcgcttt catcaaaatt tccctaccgg gcggtatcct gtttttgaag acggtaggat
     2401 ttgggtttgt ttgggggcaa tcacgcccaa attggagcaa tagatagggc tgtgtccgaa
     2461 tatgcggctc tgtgtgttga aacacggtta aaaatacgga agactttatg tccgaacaac
     2521 atatttcgac ttggaaaagt aaaatcaacg cattgggacc ggggattatg atggcttcgg
     2581 cggcggtcgg cggttcgcac ctgattgcct cgacgcaggc gggcgcgctt tacggctggc
     2641 agatcgcgct catcatcatc ctgaccaacc tcttcaaata cccgtttttc cgcttcagcg
     2701 cgcattacac gctggacacg ggcaagagcc tgattgaagg ttatgccgag aaaagccgcg
     2761 tttatttgtg ggtattcctg attttgtgca tcctctccgc cacgattaac gcgggcgcgg
     2821 tcgccattgt aaccgccgcc atcgtcaaaa tggcgattcc ctcgctgatg tttgatgccg
     2881 gcacggttgc cgccttgatt atggcatcct gcctgattat tttggtgagc ggacgttacc
     2941 gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt gagtatcgcc acgcttgccg
     3001 ccgccggcat cgctatgtcg cgcggtatgc agatgcagcc cgattttatc gagccgacac
     3061 cgtggacgct tgccggtttg ggcttcctga tcgcgctgat gggctggatg cccgcgccga
     3121 ttgaaatttc cgccatcaat tctttgtggg taaccgaaaa acaacgcatc aatccttccg
     3181 aataccgcga cgggattttt gatttcaacg tcggttatat cgccagtgcg gttttggctt
     3241 tggttttcct tgcactgggt gcgtttgtgc aatacggcaa cggcgaagca gtgcagatgg
     3301 cgggcggcaa atatatcggg caattgatca atatgtacgc cgttaccatc ggcggctggt
     3361 cgcgcccgct ggtggcgttt atcgcgtttg cctgtatgta cggcacgacg attaccgtcg
     3421 tggacggcta tgcccgtgcc attgccgaac ccgtgcgcct gctgcgcgga aaagacaaaa
     3481 cgggcaacgc cgaattcttt gcctggaata tttgggtggc gggcagcggt ttggcggtga
     3541 ttttttggtt tgacggcgta atggcgaatc tgctcaaatt tgcgatgatt gctgcttttg
     3601 tgtccgcccc tgtgtttgcc tggctgaatt accgtttggt caaaggtgat gaaaaacaca
     3661 aactcacatc aggtatgaat gcccttgcat tggcaggctt gatttatctg accggtttta
     3721 ccgttttgtt cttattgaat ttggcgggaa tgttcaaatg ataacgatgc cgtctgaaac
     3781 cgcaaaccgc tttcagacgg cattgccgcg tttatggaaa aaaatgccga catcgcgtaa
     3841 aatacgcgca aattttgtga ttcggtcagg tcgtctgaaa cagattgcgc ctgatttatt
     3901 ttttcggaaa accctatgag cgaacaaaac catccgcaaa ccgagccgca gttggacgaa
     3961 aaccaaatca tcgccctgcg ccgcgaaaaa ctgcacaaca tccgccaaca gcgcaacgcc
     4021 tatcccaacg acttcaaacg cgacagcttc gccgccgatt tgcacgccca atacggcgaa
     4081 atcggcaaag aagaactcga tccgcaaggc attcccgtca aagtggccgg ccgcatgatg
     4141 ctgaagcgtc aaatgggcaa ggcgagtttt gccaccattc aagacgtgtc cgggcaaatc
     4201 cagctttatc tgaacaacaa aggcgtgagc caagaaattt tggacgactt caaccattgg
     4261 gacttgggcg acatcgtcgg tgcggaaggc actttgttca aaaccaacca cggcgaactg
     4321 accgtacgcg tgtccgacat ccgcctgctg tctaaatccc tgcgcccgct gcccgacaaa
     4381 cacaaaggct tgagcgatca ggaaaccaaa taccgccaac gctacgtcga tttgattgcc
     4441 aacgaagaat cgcgcaatac cttcatcaaa cgcagccaaa tcatccaatc tgtgcgcaat
     4501 tttatggtga acgagcgtta tctcgaagtc gaaaccccga tgatgcaccc gattcccggc
     4561 ggcgcgacgg cgaaaccgtt cgtgacccac cacaatgctt tggacatccc gctctacctg
     4621 cgcatcgcgc ccgagctgta tctgaaacgc ttggtggtcg gcggtttgga gcgcgtgttt
     4681 gaaatcaatc gcagcttccg caacgaaggc atgtcggtgc gccacaatcc cgaatttacg
     4741 atgattgaat tttacgaagc cttctccgac tacgaacgta tgatgcagat ggcggaagac
     4801 atcatccgaa acgcatcgcg cacggtaaac ggcacggcga aaatcagcta caacggcaaa
     4861 gaagtcgatt tggaaagccc gtttgagcgt ctgaccatcc tcgaagccat caaaaaatac
     4921 aatccgcact acaccgacga gcagttgaac gatgcggaat ggctgaaaaa agaaatcgtc
     4981 aaacacggcg aaagcctgcc gccgtccccg ggcatcggca gcctgcaact cgcgctgttt
     5041 gaaggttgcg ccgagggcaa gctgtggaat ccgaccttca tcgtcgatta cccggtcgaa
     5101 gtttccccat tggcgcgcgc ttcggatacc aaacaaggtc tgaccgaacg tttcgaattg
     5161 ttcgttgtcg gccgcgaact ggcaaacggc tattccgagt tgaacgaccc cgaagaccaa
     5221 gccgaacgct tcaaagcgca agtggcgcaa aaagacgcgg gcgacgacga agccatgcat
     5281 tacgatgccg actacatccg cgcgatggag ttcggtctgc cgccgacagg cggctgcggc
     5341 atcggtatcg accgcttggt aatgctgctg accgattcgc aaaccatccg cgatgtgatt
     5401 ctgtttccgc agatgcgccc cgagtaatca taaaaacagt tgaaatggca atgccgtctg
     5461 aacctgattg gattcagacg gcattttgta tggcaggatt gatagtg
//
LOCUS       DAXHRI010000136         5402 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXHRI010000136 DAXHRI010000000
VERSION     DAXHRI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.136"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            1..254
                     /locus_tag="ACFM75_001717"
                     /pseudo
     CDS             1..254
                     /locus_tag="ACFM75_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            660..1715
                     /locus_tag="ACFM75_001718"
     CDS             660..1715
                     /locus_tag="ACFM75_001718"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6256225.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESGLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITEEKLEPAKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            1730..3376
                     /locus_tag="ACFM75_001719"
     CDS             1730..3376
                     /locus_tag="ACFM75_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC6256226.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            3380..3802
                     /locus_tag="ACFM75_001720"
     CDS             3380..3802
                     /locus_tag="ACFM75_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC6256227.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEILVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAIGRERLNRLVREIKDAV"
     gene            complement(3825..>5402)
                     /locus_tag="ACFM75_001721"
     CDS             complement(3825..>5402)
                     /locus_tag="ACFM75_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6256228.1"
                     /translation="QEGEQSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWL
                     KTAERENPRLLFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVT
                     MTVPLTVLNRLHAPSLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLENNP
                     DRQAAIIPQLAHFIAKSASDMRILEQIDQDAWAAQDLPEHCYLNLRIIDDGGQELAGG
                     RKLHELQQQLGKAAATTFRDNTQEFERDNVTTWDIGILPESIKFARGKQQLTGYLGLQ
                     KEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHI
                     NADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAA
                     AYAELNGKLGKHPLTHLLRLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSS
                     NPARDAAREADIQELEQMWQEKNDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKT
                     PYPVSVKRLLKMWEDLN"
BASE COUNT         1518 a         1020 c         1417 g         1447 t
ORIGIN      
        1 atcccgaact cgagcacagc ctcatcaccc gcatcgactt caacctgttt tgccgctttg
       61 acgaactgag catccccgat tacagcacct tatgccgtta ccgcaactgg ctggcgcaag
      121 acgacaccct gtccgaattg ctcaaactga ttaaccgcca actgaccgaa aaaaacctaa
      181 aagtagagaa agcatccgct gccgtcgttg acgccaccat tattcagacc gccggcagca
      241 aacagcgcca ggcccgataa cgcccggcgg aagtttttcg gacatcgcga accttgccct
      301 gctgaaaata caaaaggccg cgttcggcga tatggggcgg gaacaggtga ggagggtgga
      361 tacggggcat ggttggcttc caagaagttg ttgaaatatg gggagtttac tggttcgggc
      421 gacgtttggg gagggggtgg gaattcttgt tttgggaagt ggaaaaaggt cgtctgaaaa
      481 tgggtttcag acgacctttc tgtgaagtgt gcatttgaag acatccgctc gtttggagga
      541 aattaagttc tgcttattta gatgcatgaa ttttaataaa atctcaaatt atttttgata
      601 agcaataaat tatatgttat ataaagttag ttctcactag tctaattatt taaatagaaa
      661 tgaaaaacag taagttaaag gcggttgatt ttttctgtgg cggaggtgga atgagctatg
      721 gaatgcaatc cgccggtatt caggttttag ccgggataga ttacgaaccc agctgcaaag
      781 aaacatatga agccaatata aatgccaaat ttatacaggc tgatgttttt gaattgcagc
      841 ctgaaacctt ggaaaaagaa ctcgggttaa aaaagaatga tgatgatttg attcttatag
      901 gttgtagtcc ttgtcaatat tggagtgtta ttcaaacaga caaaagaaaa tcggaaaaat
      961 ccaagagttt acttttggag tttcagcggt ttgtagaata ttttaatccc ggatatgttg
     1021 ttgtagagaa cgtaccggga attctgagca ggatgaaaga aagcgggctt gataatttca
     1081 taaaattatt agaagaaaaa ggatttaccg tccatttcgg tattcacaat acggctgatt
     1141 acggaattcc ccaaagtcgt aagaggttta ctttgattgc aaacagaata accgaagaga
     1201 agctggaacc agccaagtat tcgggcaaac ggcttacggt acgcgatgtt ttaggaatgg
     1261 aaaatggctt tcccaaaatt attgcaggac accaagacga aacggatttt atgcatagct
     1321 gtgcgggatt atctgatatc aacttgaaac gattggcttt gatacctaaa aacggaggaa
     1381 accgtttggc ttttgcgcat attcccgaat tacagctgga atgttttatc ggaaaagaca
     1441 atagttttaa agacacgttt ggtaggctat ggtgggataa gcccgcacct accataacga
     1501 ccaaattctt tagtatttcc aacgggaggt ttgcccatcc tgaagaagat agggcgttat
     1561 cactgcgcga aggtgcaaca ttgcaatcgt tccctcgtaa ctatgtcttt aaagcgggca
     1621 gcagggacaa gatagcccgt ttgattggta atgccgttcc tccgatgtat gcggaaaaaa
     1681 taggcagggc aattgttgat aatatcgaat gttagaaaag gagaaaaaca tgtctgaaga
     1741 aaaattgaaa atgagtttcg agccaaccgt aatcgaacat ttgggtgtaa agatgtattc
     1801 gcacactgtt cctgcgattg ccgagttgat agcgaatgcc tacgatgcat gtgctacgga
     1861 agtggaagtt aggttattcg ataaaccgga gcataaaatc gttatcaaag ataatggtat
     1921 aggaatgagc ttcgatgaaa tcaatgattt ttatttgaga atcggtcgga acagaaggga
     1981 agaaaaacaa gcttccccgt gcggaagaat tccaacgggt aaaaaaggcc ttggtaaatt
     2041 ggcattattc gggcttggca acaaaattga aatttctact atccagggaa acgaaagggt
     2101 tacttttact ttggattatg cagagattcg aagaagcaag ggtatttatc aaccggagtt
     2161 tcgaaaagaa tctgttgaat ccaatatcga aagcgggaca accataactt taaccgaact
     2221 gacgaaaaag caaggatatc cgttagataa ttatgtagag catctttccc gcttgtttga
     2281 ttttccggct caggatttta aaatcaaagt aagcttgaac ggctctgaac ctaaaatcat
     2341 tgatggaaat ctaaaatatg atcttgttac cccacaattc gaatgggaat accaggattt
     2401 agcaaccaat atttcatcgt tatcttcaaa attcgaacag tatgaataca gcggattaat
     2461 acaaggtaag ttcattacaa cggaaaaacc tttaaagaat aatatgaaag gtattacctt
     2521 gtttgccaac ggcagaatgg taaatatgcc cgagtttttc actgatagcg aatccagcca
     2581 tttctattca tatttgactg gatggctgaa tgtggacttt atagataatg acgatgaaga
     2641 tttaattgct accgacagga catcattaga ctggaagcat tcaaaaacct caaaattacg
     2701 tgttttcctt ggggagatcg tccgagccat cgggcaagat tggagaaaaa gaagaaaaaa
     2761 agagaaagat gacgaagtga aaggcgaaag cggagttgat atcgaagatt ggaaaaacaa
     2821 attgccagaa aaggaaaggg aaccagtaga agttattttg aatcgtttgg aagattcgga
     2881 gcttacaaat aaagagcaag cggaagtaat ctctgcctta catagtatta ttccagaata
     2941 tccttactat cattggcggc atttgcatca ggatttgcat actgcctgta atgattttta
     3001 caatgaaaag aaggattatt taagtgctgc aatcgaggca gttaaagttt tcgaagataa
     3061 ggttcagaag caaacaggtc ttcatagcat agacggaaga gaattgattg aacaagcttt
     3121 cgggagtaaa aattctatac ttctattaac gaataacaaa acgaaggcgg aacaaaattt
     3181 agaagatggt cttgaacagc ttgcatgcgg aacatggact ggatttagaa atcctgtaca
     3241 acatgagtta agagcaaatt tatctcctag tatttttaat gataaggatg ctttggattt
     3301 aataagcttg gtttcgtatt tgttaagaaa agtggaacag acaaaaaaac gatcgaaggt
     3361 ggtatcttcc aagtaattta tgaccgatat tttcactaca tccaaacgtt ctttcgtgat
     3421 gtcaaaaatc catagcaaag aaaccaaacc ggaaatattg gtaaggaaat ttcttttctc
     3481 ccaaggtttt cgttatcgga aaaacgataa acgctatgca ggcaaaccgg atatcgtttt
     3541 gccgaaatat aaaacagtcg tgtttataca cggctgtttt tggtacgggc attcttgtaa
     3601 taaaggacat atcccgaaaa gcaatacgga tttttggttg gaaaaaatca caaaaaattg
     3661 cgaacgtgat attaaaaatg aaaccgagct ggaaaagatc ggtttcaaag tgattgtcgt
     3721 ttgggagtgc gaattaaaaa ataaagctat tggtagggag cgattaaatc ggttggtcag
     3781 ggaaataaaa gatgccgtct gaaaaaatca gacggcatct tcctttaatt aagatcctcc
     3841 cacatcttca acaaccgctt caccgacacc ggataaggcg tcttcaattc ctgcgcgaac
     3901 agcgacaccc tcaattcttc gatcatccat ttaaacgcgg tgaggtcgtc tgaaacgggt
     3961 agtccttgtt tcaccaaacc gtcgtttttt tcctgccaca tttgttccaa ttcctgtata
     4021 tcggcttcgc gggctgcgtc gcgggcgggg ttgctgctgt atttttcgag gcgcagggtc
     4081 atggctttga ggtagatggg gaggcgcggc cattgtgccc acggggtgcg ggtggcgaag
     4141 ccggcggcga gcagggtttg caggcgtagt cttagaaggt gggtcaatgg gtgtttgccg
     4201 agtttgccgt tcagttcggc gtaggcggcg gcggtttcct gcaggtagcg gctgagggct
     4261 tctttgacgg cgggcaggcg gctgcgggcg cgtttgattt gttctttgaa ggctttttcg
     4321 ttgcgcggca gctcgtcttc gccgataaag gcgcggtcac aaacggcttg ggtgaggtcg
     4381 tcgcgcaggg tgtcggcgtt gatgtgtttg agcagcatgg cggcttgggt gaagccctgg
     4441 atgcctttgt tcaaatcttt cacctgctct tttaattgca gcttcattaa ttcgatgaca
     4501 ccttgacggt gtgcctgttc ggcggcttcg gtggtgtcaa ataggcgcag ggcgatgcgg
     4561 ccgtcttttt ctttttgcag gccgagatag ccggtaagct gttgtttgcc gcgggcgaat
     4621 ttgatggatt cgggcaggat gccgatgtcc catgtggtta cgttgtcgcg ctcgaattct
     4681 tgggtgttgt cgcggaaggt ggtggcggcg gctttgccga gctgttgttg caattcgtgc
     4741 agtttgcggc cgccggcaag ctcttgtccg ccgtcgtcga taatgcgcag attcagatag
     4801 cagtgttcgg gcaagtcttg cgccgcccat gcgtcttggt cgatttgctc gagaatccgc
     4861 atatcgctgg cgcttttggc gataaagtgc gccaactggg gaatgatggc cgcttggcgg
     4921 tcgggattgt tttcgaggaa tttggtgatg aaatcgggta cgggcacgca aatgcggcgg
     4981 atttgcttcg gcagtgcttt aatcagcagc tggatttttt cgcgcaacat accgggcacc
     5041 agccattcga gcgacggcgc atgcaggcgg ttgaggacgg tcagcggcac ggtcatggtc
     5101 accccgtcga gcggatggtg tggctcaaag cggtaggaaa gtttgaactt gccgtctgcg
     5161 gtttgccaga atttggggaa ctgttcttcg gtaatgtgtg cggcggcgtg ttgcatcaaa
     5221 tcgtcgcggc tgaggaacag caggcgcggg ttctcgcgtt cggcggtttt gagccaggct
     5281 tggaaggtgc ggatgtcggc gagggggagg ggtttcagac ggtctttttg gggtgctggc
     5341 tgcttttggg gttgagggtc gtttgaaacc gctgaagctg cggcactctg ttccccctcc
     5401 tg
//
LOCUS       DAXHRI010000137         5368 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXHRI010000137 DAXHRI010000000
VERSION     DAXHRI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.137"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            126..436
                     /locus_tag="ACFM75_001722"
                     /pseudo
     CDS             126..436
                     /locus_tag="ACFM75_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(493..1551)
                     /gene="trpD"
                     /locus_tag="ACFM75_001723"
     CDS             complement(493..1551)
                     /gene="trpD"
                     /locus_tag="ACFM75_001723"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC6256229.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1615..2205)
                     /locus_tag="ACFM75_001724"
     CDS             complement(1615..2205)
                     /locus_tag="ACFM75_001724"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC6256230.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            2627..4903
                     /gene="znuD"
                     /locus_tag="ACFM75_001725"
                     /gene_synonym="tdfJ"
     CDS             2627..4903
                     /gene="znuD"
                     /locus_tag="ACFM75_001725"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC6256231.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVGLETVTVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDVDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLHNKR
                     YELRAEWKQPLPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNNVQHYSFFGVEQAEWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
BASE COUNT         1384 a         1491 c         1242 g         1251 t
ORIGIN      
        1 cagacacttt aagcccatga aaatggtgcg aatcctcaaa aacgggcaaa aaaaagccct
       61 gttacttgta gaaagtaaag ggcgttaatt tttgttaatc gtcccttctt agggacgcaa
      121 tatataaggt ttataccgtc gttgttccta atgcgcaatc agcgacattg ttgccaatta
      181 tccgaaagaa agttaagcct gatggcattg tatacacgga tacctttcgt agttatgatg
      241 tgcttgatat tagtgaattt agccatttac gtaagtttaa cggcattccc aaagagcatt
      301 tggggctgca tttaaagaaa tgccaatggc attttaaata gaatctcaaa ttcagatttt
      361 aagacaatta gttaatggga atttggtctg gttatctagt acagccccaa gtttttatat
      421 aaaaacagtt tggtaagttc tcttcgtgaa gtgcttatgt tttgccacaa aagttccata
      481 atcatggcgg catcacccaa gctccatctt tccaagaaaa tggcatcttt gttgtggttg
      541 aaaaccgaca aactcctctt ttttcgattt ggccctgcct gaatcgatgg cttcccgtgc
      601 ggcagatatg ccgtctgaaa gcgaagcagc gacatttccg gcatataggg cggcggcggt
      661 attaagcaat acgatatcgc gcgcagcccc ttctttccca tccagcacct cattcatttt
      721 caacaaagat tcctgagtat tggcaacttt gatttcatcc aaattgcggc gggtttcgat
      781 accgaaatct tctgggcgga tgtcgtattc gctgattttt ccgtctttga gttcggcaac
      841 gcgcgttttg cccgtcagtg taatttcatc caaaccgccc tccccgcaaa cgactaaaac
      901 gtgttttgaa ccgagttgtt gcaagacccg cgacaaaatg ccgcacaaat cggtgtggaa
      961 cacgcccaaa agctggttcg gcgcgcccgc aggattggtc aacggaccca atatgttgaa
     1021 aatgcttcgg aaaccgagcg aacggcgtac cggggcgaca tggcgcatgg cactgtggtg
     1081 attgggggcg aacataaacc ctataccggt ctggctgata ctttgggcaa cctgttcggg
     1141 agtcaggttg aggtttgcgc ccatctgctc caccacgtca gccgcaccgc tggaggaaga
     1201 gaccgaccgg cctccgtgtt tggcaacctt cgcgcctgcc gctgcggcaa caaacatcga
     1261 agtcgtcgaa atattgaagg ttttcgcgcc atccccgccc gtaccgacga tatcgaccag
     1321 cccctctgca ttctccagcg gcacttttgt cgcaaactcg cgcatgacgg ctgcagctgc
     1381 ggtaatttcg gaaacggttt caaccttgat acgcaatcct gtcaaaatgg ccgctatctg
     1441 ctccggcaga acctgtcctc tcataatctg acgcatcaag tcggtcattt catcgtaaaa
     1501 caactcgtta ttgctgatta atcgttcgat ggcctgttgc ggtgtaatca ttttttgtcc
     1561 tccgttcaat attcggacga aaatgccgtc tgaagggctt cagacggcat cacgtcagat
     1621 tttttgcggt ttgaagtttt gaaattcgat taaaaaattg tttaacatat catgtccgtg
     1681 ctcggtcaag agggcttcgg ggtggaactg cacgccctcg acggcatatt ccttatggcg
     1741 cacacccata atctcgccgt cctcagtcca agccgttact tccaaacatt cgggcatcgt
     1801 attccgatcg ataacgaggc tgtgataacg agtacaggta accggattgg gcaaaccctt
     1861 aaacataccc ttgcccgaat gggacacggg cgacacctta ccgtgcatca gcgttttggc
     1921 gcggactatc ctgccgccga acgcctcgcc tatcgtctga tgcccgaggc acacgcccat
     1981 aatcggcagc cggccggcga aatggcgcat cgccgccacg gaaatccccg cttctttggg
     2041 cgaacacggg ccggggccga taacgagata ttgcggattc aatgcctcga tttcctccaa
     2101 cgtaatatca tcgttgcggc gcacggcaac ttcctgcccc aattcagtga aatactggac
     2161 gatgttgtaa gtaaaactgt cgtaattgtc gataaacaaa agcattttgt gattaaccta
     2221 ttgatttata atttgtttgt tattaactgc atttccgata cccccatcca taccctctta
     2281 tatcttagcg tgcccgatgc gccctcgtga acctggcgca gagcctcgcg ttctgacaag
     2341 tacgccattt tgccaccttt tcgaccgttc gggaacgtgc cgtttttatg cactcataaa
     2401 ttttggttat cccgcagccg aacataatgg gcaaacacag cggaatctgc gtcaacatca
     2461 accgcgcccc cgaaggacgg cggatacaaa tggacaccgc ccgatgccgc aaccgtttca
     2521 tttgaaaaac ttttgcctat gccctctgag tgttctcaaa atcttatgat ataaaaaatg
     2581 taatgttata taataacaaa cttttgaaat gcgacgagaa gccaaaatgg cacaaactac
     2641 actcaaaccc attgttttat caattctttt aatcaacaca cccctcctcg cccaagcgca
     2701 tgaaactgag caatcggtgg gcttggaaac ggttaccgtc gtcggcaaaa gccgtccgcg
     2761 cgccacatcc gggctgctgc acacttcgac cgcctccgac aaaatcatca gcggcgacac
     2821 cttgcgacaa aaagccgtca acttgggcga tgctttggac ggcgtgccgg gcattcacgc
     2881 ctcgcaatac ggcggcggcg cgtccgctcc cgttattcgc ggtcaaacag gcaggcggat
     2941 taaagtgttg aaccatcacg gcgaaacagg cgatatggcg gatttttcgc ccgatcacgc
     3001 cattatggta gataccgcct tgtcgcaaca ggtcgaaatc ctgcgcgggc cggttacgct
     3061 cttgtacagc tcgggcaatg tggcggggct ggtcgatgtt gccgatggca aaatccccga
     3121 aaaaatgcct gaaaacggcg tatcgggcga actcggattg cgtttgagca gcggcaatct
     3181 ggaaaaactc acgtccggcg gcatcaatat cggtttgggc aaaaactttg tattgcacac
     3241 ggaagggctg taccgcaaat cgggggatta cgccgtaccg cgttaccgca atctgaaacg
     3301 cctgcccgac agccacgccg attcgcaaac gggcagcatc gggctgtctt gggttggcga
     3361 aaaaggtttt atcggcgtag cgtacagcga ccgtcgcgac caatatggtc tgcctgccca
     3421 cagccacgaa tacgatgatt gccacgccga catcatctgg caaaagagct tgattaacaa
     3481 acgctattta cagctttatc cgcacctgtt gaccgaagaa gacgtcgatt acgacaatcc
     3541 gggcttgagc tgcggctttc acgacgacga taatgcacac gcgcataccc atagcggcag
     3601 accgtggata gatttacaca acaaacgtta cgaactccgc gccgaatgga agcagccgct
     3661 ccccggtttt gaagccctgc gcgtacacct gaaccgcaac gactaccgcc acgacgaaaa
     3721 agcaggcgat gcggtcgaaa acttttttaa caaccaaacg caaaacgccc gcatcgagtt
     3781 gcgccaccaa cccataggcc gtctgaaagg cagctggggc gtgcaatatt tacaacaaaa
     3841 atccagtgct ttatctgcca catccgaagc ggtcaaacaa ccgatgctgc ttgacaacaa
     3901 tgtgcaacat tacagctttt tcggtgtaga acaggcagaa tgggacaact tcacgcttga
     3961 aggcggcgta cgcgtggaaa aacaaaaagc ctccatccgg tacgacaaag cattgattga
     4021 tcgggaaaac tactacaacc accccctgcc cgacctcggc gcgcaccgcc aaaccgcccg
     4081 ctcgttcgca ctttcgggca actggtattt cacgccgcaa cacaaactca gcctgaccgc
     4141 ctcccatcag gaacgcctgc cgtcaacgca agagctgtac gcacacggca aacacgttgc
     4201 caccaacact tttgaagtcg gcaacaaaca cctgaacaaa gagcgttcca acaacatcga
     4261 actcgcgttg ggctacgaag gcgaccgctg gcaatacaat ctggcactct accgcaaccg
     4321 cttcggcaac tacatttacg cccaaacctt aaacgacgga cgcggcccca aatccatcga
     4381 agacgacagc gaaatgaagc tcgtgcgcta caaccaatcc ggtgcggact tctacggcgc
     4441 ggaaggcgaa atctacttca aaccgacacc gcgctaccgc atcggcgttt ccggcgacta
     4501 tgtacgaggc cgtctgaaaa acctgccgtc cctacccggc agggaagatg cctacggcaa
     4561 ccgtcctttc atcgcgcagg acgaccaaaa cgcccctcgc gttccggctg cgcgcctcgg
     4621 cttccacctg aaagcctcgc tgaccgaccg catcgatgcc aatttggact actaccgcgt
     4681 gtttgcccaa aacaaactcg cccgctacga aacgcgcacg cccggacacc atatgctcaa
     4741 cctcggcgca aactaccgcc gcaatacgcg ctatggcgag tggaattggt acgtcaaagc
     4801 cgacaacctg ctcaaccaat ccgtttacgc ccacagcagc ttcctctctg atacgccaca
     4861 aatgggccgc agctttaccg gtggcgtaaa cgtgaagttt taaaatcgga caggcaaaca
     4921 aaaaaggttt tcagacggca ttctgaaaac cttttttctt tttaatcaat tttttaggaa
     4981 atatggcaaa gacaaagaac aaaccactat gccatcattc ccattttttc tgtttcatca
     5041 ttcttgcttt cacgccatca ttctcgcaaa agcagaaaaa tcaaaagcaa aaacccaaga
     5101 tcccgtcatt cccgcgcagg cgggaatcca attcattcgg tttcagccgt ttccgataaa
     5161 tcgccttagc atttgggttc tagattccca ctttcgtggg aatgacggcg taaaggtttc
     5221 tgttttttaa gtgttaggta actaatgagt cgtcattccc gcgcaggcgg gaatccaatt
     5281 cgttcggttt cagccgtttc cgataaatca ctgtaacgtt aagttcccgg attcccactt
     5341 tcgtgggaat gacggtgtaa aggtttct
//
LOCUS       DAXHRI010000138         5338 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXHRI010000138 DAXHRI010000000
VERSION     DAXHRI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.138"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(180..3011)
                     /gene="lbpA"
                     /locus_tag="ACFM75_001726"
                     /gene_synonym="tbpA"
     CDS             complement(180..3011)
                     /gene="lbpA"
                     /locus_tag="ACFM75_001726"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC6256232.1"
                     /translation="MNKKHGFQLTLTALAVATAFLAYAAQAGGAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSNKTGQFFLVEGENTLK
                     PVAKLAGYGKYLNNQLNRWVKERIEQNKPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISESTG
                     FDEKNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3008..5182)
                     /locus_tag="ACFM75_001727"
     CDS             complement(3008..5182)
                     /locus_tag="ACFM75_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6256233.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGPSVETTPVNQPAVGAAMRLPRRNIAAFDKNGNEIPNSKQAEEYLPLKEE
                     DILFLDGTPQEQADKLKKKINERYPNVKIYTSDSKDAEYKYVRAGYVYTRFGTDEIEQ
                     NSGGKQVTHRLGYDGFVYYSGERPSQSLPSAGTVKYSGNWQYMTDVKRHRAGKAVGID
                     NLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDQKTLNGKLIKNQYVQRKTDEK
                     KPLTIYDITAKLDGNRFTGSAKVNPDLAKNPAGNERLFFHADADQRLEGGFFGDNGEE
                     LAGRFISNDKSVFGVFAGKQKTETANASDTNPALPFGKHTKILDSLKISVDEATDSNA
                     RKFAISSMPDFGHPDKLLVEGREIPLVSQEKTIKLADGRKMTVSACCDFLTYVKLGRI
                     KTDRPAAKPKAQDEDSEDEIGESEENEDEISDEEGTEDEVVEDEGSEEDEVSESDNGE
                     NEEEIAEEETEEEVDEAEEEKVEEPEEELPAEEGNGGSGSILPTPEAPKGRDIDLFLK
                     GIRTAEADIPKTGKAHYTGTWEARIGVPDKKDDQPGTTFIQKDSYANQGAKAEFDVDF
                     GAKSLSGKLTEKNDTHPAVYIENGVIDGNGFHATARTRDNGIDLSGQGSTSPQSFKAN
                     NLLVTGGFYGPQAAELGGNIIDSDRKFGAVFGAKKDNKEVEK"
BASE COUNT         1135 a         1429 c         1253 g         1521 t
ORIGIN      
        1 ttttcgagtt tcagtcattc ccgataaatt gccttagcat tgaatgtcta gattcccgcc
       61 tgcgcgggaa tgacggcgga aatcttgttt atattgaatc aaaaaaacct gcaccttaat
      121 cagttggcgg tttagtccga cttttggggt gcagatcaag ctttcagacg gtatttcctt
      181 taaaacttca tttcaagcgc gaggctgaag ttcctgcccg gtgcggcata ccttccatag
      241 ttgctgtcgc cgccgtgccg gtttgccgtg ctttccgcgg tctggcgcaa ggattcccaa
      301 gtaacgtagc ggtagttgcc gatattgtag atagccgccc tcaaggtcag ccgttttttc
      361 agattcagat aggcggaaac gtctgccgtc gaccaagaag acgacgctct ttttgtcgaa
      421 tatcgttttt gatcgcctgc cagataagca agctcgtcag ggtttttccc tttggaatag
      481 gtcagcataa tgtttgcgcc ccatttcccc tctggctggt cgtatccgaa ccccaaaaca
      541 taacgcgacg gctgtaccgc atccaaagca tagctgcgga gggacaagtc cggccggttg
      601 gataccgatt tcggtttgat gcggttgtac gccaatgtgg tgtacaaacc ttcgggcagt
      661 ttgccgtaca caccgttcca gtcgattttt cccaatatat taacgccttg gagcgacata
      721 ttttgggcat tgtaataatc gcgtatatca atctctgtca attgtcctgc ctgattcggc
      781 aatttggttt tgtgatcggc aacggcaatc atatcggtat aacggttgcg gaagctgctg
      841 atttccaaaa agccgaaatc gcccttccac tgcaaaccga tttcgcggtt ggctgccttt
      901 tccgatttca gggcgggacg ctgccagcct ttcggataat cgtgataaat gtctatcccg
      961 aaaagttctt ggaatgaggg cgttctgaag ccgctggagg cgcggtaaga caaagaaaaa
     1021 tgccggttcg gtttgaacac gatgccgctg ttccacgaac ggtcaacata ccgcccgctg
     1081 cggacgagtt cttccgacgt ggtgaagttt ttccggtcat acctgccgcc caagctgaaa
     1141 tcaaaatatt tgccgattga aaaacggtcg ttcaaagaaa tatggatatt gctgccattg
     1201 atttttcttg gcacgcattt gcgggaacgc agggtttcga tgtagccgca gaccgaccct
     1261 tcgacgactt cgggcttacc caaaagatac ttatcttgat tcttttcatc gaatcccgtg
     1321 gattccgaaa tccttgccgc attgtgggaa agctgttcgg ggcgggagat cgcgttggaa
     1381 gcatcgtaac cgaagcccaa agtcagatgg tgtttcgtcc atttgttttt cagcgatttc
     1441 tcaaacgagg cattcaaaac attgtgctgt tcgcggtagt ggaaacggtc gctgctgtcg
     1501 taggaatacg gtttgtccgc cgacgcgcgg caggatttgt ccacagcagg atacacggcg
     1561 caattcagct tcagcgtgtt gttgtcggtt gccacgccct gtttgtcaaa cgacaacacc
     1621 gccttatccg cccaattgtc agaatacgct tcgttttcat aacggtacag caaacccata
     1681 cggcggcggc ggtgatgttc gtcaataaat ttggtgcggg aatatttcaa acctatgccc
     1741 ttgaccaact ttttatcgcc cttccactct tctatattcg gcacaaaata caagccgtcg
     1801 cggaaatcgt cgccgtcgta caccccactc ttgtctctaa acttttccgc ctcgtccgta
     1861 ccgtaatact gtttttccgt catatcgcgg atgtcgtaac gctgtttggt atcctcaaac
     1921 acgccgccga cataatgcct gccgccgaag cggtagccca gcttggcaag ccaagagccg
     1981 ctgcggtaat ccatcggatc gggcaatatc ctgccgccgc ccgtgtaagc ttgggcggac
     2041 aggttttcgt ggcgcgcctg cgcctcccgc acctgcgcct cttcttcagc acttaaaggc
     2101 ttattttgtt caatacgttc ttttacccag cggttgagct ggttgttcaa atatttcccg
     2161 tagcccgcca attttgccac gggcttgagt gtattctcgc cctctactaa gaaaaattgc
     2221 cctgttttat tgctttgaat atcgtgtgtc tggcggaacg catccaaacg gtttatgccg
     2281 tatgccaccc cgtctgcaat atcgccgtgc gggcgcgttt cccgcccttg acgttcggta
     2341 cggattagca gcccttccca accgtctttg ctgaaccccg cgccgagcga cttcataaat
     2401 tggcggtttt tactgccgta ggcggttttt gcctgtatcc cccagctttt gccgtctgaa
     2461 atcaggtctg ccgcctcttt ggtgcggaag gcgaccgcgc cgccgagcgc accgctgccg
     2521 tgatcggacg aaccggcgcc tttgtcgatt tccaccgtgc tgatgttttc atattcgatt
     2581 tcgttgattg caccgctgcc gccgcgtccg ccgtatccgc tcaacgatcc ctgcacggta
     2641 aacgcctgta tttgggcaac gccgtcaacc gaaaccgcca cacggttttt atccacgccg
     2701 cgtatcgagt agccgccgct cgcgccgttg ccctgttcga caaccgccac gcccggatcg
     2761 tagcgcgtca ggtcgcggat accgagtacc tgttctttgt tcaacgtttc cgacgttttg
     2821 acgattttgc ccaaaccggt cgcctctttc gatcgccgtc ccactttggc ggcacggacg
     2881 gtaacctctt tcagggattg ggtctgcgcg gcatcaggtg ccgccccccc cgcttgggca
     2941 gcataagcca gaaaagcggt tgcaacagcc aaggcagtca gggtaagctg aaaaccgtgt
     3001 ttcttattca tttttccacc tctttattat ctttcttcgc accgaatacc gcgccgaatt
     3061 tccggtcgct gtcgataata ttaccgccca attccgccgc ctgcggtcca taaaagccgc
     3121 ctgttacaag aagattgttg gctttgaagc tctgcgggct ggtcgaaccc tgcccagaaa
     3181 ggtcaatacc gttatcccga gtgcgtgctg tcgcgtggaa accgttgcca tcaatcacac
     3241 cgttttcaat ataaacagcg gggtgtgtat catttttttc tgtcaactta cctgaaagcg
     3301 acttcgcgcc gaaatcaacg tcaaattctg cttttgcccc ttgattcgca tagctatcct
     3361 tttgaatgaa cgtagtgcca ggctgatcgt ctttcttatc tggtacgccg atacgcgctt
     3421 cccaagtgcc ggtatagtgt gcttttccag tttttggaat gtcggcttcc gccgtgcgga
     3481 tacctttcag gaaaaggtcg atgtccctgc ctttaggggc ttccggagtg ggcaggatgc
     3541 tgcctgaacc gccgttgcct tcttctgccg gcaattcttc ttcgggttct tcaactttct
     3601 cctcttcggc ttcatcaact tcttcttcgg tttcttcttc ggcgatttct tcttcgtttt
     3661 caccgttatc actttcggaa acttcgtctt cttcgctgcc ttcatcttct acgacttcat
     3721 cttccgtgcc ttcttcatcg ctgatttcgt cttcgttttc ttcgctttca ccaatttcat
     3781 cttcggaatc ttcgtcctgt gcctttggtt tggcggcggg gcggtcggtt tttatccgtc
     3841 cgagtttcac ataggtcaga aaatcgcaac aagcactgac ggtcattttc ctgccgtcgg
     3901 caagcttgat ggttttctct tggctaacca aaggaatttc acgcccttcg acaagaagct
     3961 tgtcgggatg accaaaatcg ggcatagagg aaatggcaaa cttacgggca ttgctatcag
     4021 tcgcctcgtc aacggaaatt tttagagaat ccaagatttt ggtgtgtttt ccaaacggca
     4081 gggcaggatt tgtatctgat gcgtttgctg tctctgtttt ttgtttgcct gcgaatacgc
     4141 cgaatacgct tttgtcgttg ctgataaatc gtccggcaag ctcttctccg ttatcgccga
     4201 aaaaaccgcc ctcaagccgc tgatcggcat cggcatggaa aaacaaacgc tcattaccgg
     4261 cagggttttt cgctaaatca ggattgacct tggcactgcc ggtaaagcgg ttgccgtcca
     4321 attttgcagt aatgtcgtaa atggtcagcg gtttcttttc atcggttttc ctttgcacat
     4381 actgattttt aatcagcttg ccattcagag ttttttggtc gaaatcaacc gtatatttgg
     4441 caggatgctt ttccctttcg tcgacatcct tagccgcata agaagttgca ccaacatcgt
     4501 taccataaaa tgtgatataa cccaaattgt caatgccaac cgccttacct gctcgatgac
     4561 gtttgacatc ggtcatatat tgccagtttc cggaatattt caccgttccc gcgctcggta
     4621 aagattggga aggacgttct ccagaataat atacaaaacc atcataacct aagcggtggg
     4681 taacctgctt accacctgag ttctgttcga tttcatctgt tccaaatcta gtataaacat
     4741 atccggcccg gacatattta tactcagcat cttttgaatc ggacgtgtag atttttacat
     4801 taggataccg ttcgttgatt ttctttttaa gtttgtcggc ctgttcttgc ggcgtaccgt
     4861 ctaaaaacag gatatcctcc tctttgagcg gcagatactc ctctgcctgc ttactattgg
     4921 gaatttcatt accattttta tcaaaagctg cgatattccg ccttggcagc cgcattgccg
     4981 caccgacggc gggctgattg accggcgtgg tttctaccga aggcccggca gggggcggag
     5041 tgggaacgtc cttagacttg aaagtgacgg ggtacgcggt cggcgttgat tcgacaacag
     5101 gctgcacgcc gaaattgccg ccgatacaag atgccaaaag taagggcaac aagacaatgc
     5161 cgccataatt cggtttacac atccctactt ttcctcctat ttgattaata ataattatca
     5221 ttatattaat atgtacagat aatatcaagc cgtttttata gtgaattaac aaaaatccgg
     5281 acaaggcgac gagccgcaga cagtacagat acattccgtc attcccacga acctacat
//
LOCUS       DAXHRI010000139         5295 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXHRI010000139 DAXHRI010000000
VERSION     DAXHRI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.139"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..338
                     /locus_tag="ACFM75_001728"
     CDS             <1..338
                     /locus_tag="ACFM75_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6256234.1"
                     /translation="FFDTEPDRGKIEHCACLAMIHEEISAMPMGYETLIGDMGSALSG
                     GQKQRIVLARALYCEPKILFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVE
                     SADRKMSLG"
     gene            complement(365..1585)
                     /locus_tag="ACFM75_001729"
     CDS             complement(365..1585)
                     /locus_tag="ACFM75_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC6256235.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSMMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKRQ"
     gene            complement(1600..2025)
                     /locus_tag="ACFM75_001730"
     CDS             complement(1600..2025)
                     /locus_tag="ACFM75_001730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256236.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2130..2789
                     /gene="queC"
                     /locus_tag="ACFM75_001731"
     CDS             2130..2789
                     /gene="queC"
                     /locus_tag="ACFM75_001731"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC6256237.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYKGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2817..3335
                     /locus_tag="ACFM75_001732"
     CDS             2817..3335
                     /locus_tag="ACFM75_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC6256238.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPTLKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTEMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3342..3764
                     /gene="queD"
                     /locus_tag="ACFM75_001733"
     CDS             3342..3764
                     /gene="queD"
                     /locus_tag="ACFM75_001733"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC6256239.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4033..4404
                     /locus_tag="ACFM75_001734"
     CDS             4033..4404
                     /locus_tag="ACFM75_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC6256240.1"
                     /translation="MKLLSTLLVLLVTAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4401..5039
                     /locus_tag="ACFM75_001735"
     CDS             4401..5039
                     /locus_tag="ACFM75_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC6256241.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
BASE COUNT         1363 a         1526 c         1320 g         1086 t
ORIGIN      
        1 gttttttcga tacggaaccg gatagaggaa aaatcgaaca ttgcgcctgc cttgctatga
       61 ttcacgaaga aatctccgcc atgcctatgg gctatgaaac cttgatcggc gatatgggca
      121 gcgcactgtc aggcggacaa aaacaacgca tcgtattggc gcgggcctta tattgcgaac
      181 cgaaaatcct atttttagat gcagcgacca gccatttgga tattgccaat gaaaaagcag
      241 tcaatgcaaa cttgaatggc ttgtctatca taaaaattat ggcggcacac agaaaggaaa
      301 cggtggaatc agcagatagg aaaatgtctt taggataaaa atacagtttc aaaaatactc
      361 aagactactg ccgttttttc gcctgagcgt caaactctgc cagcgtcatg ttcaaagtct
      421 gcaaacacgg tgtcattacc gcatcgacag cttggttcac atgatccctt tccacaggca
      481 acggacggta aacgaagagc ttgaagagtt cgttcaactc aatcgaatcc gcccccgttt
      541 tcaacaccca accctgtctg ccggaataga tgtagccgtg ccgcgccagc ttttccaaaa
      601 gctcgcccaa ctcgtcgtag cccatattga tatgccgtct gaactcctga acaggcaagg
      661 ctttgccttc tttttgcgcc gcatccagaa gcagcaggat tttcaacacg tcgtcaaacc
      721 gtccgcgcga gtcgaagccc ctgcggaacg cttctccctg ccagtaggag agtgaagaag
      781 tcagcaccgc gccgcccaag accagcgtcc acaacaggtt cagccacaac agaaaaaacg
      841 gcacggcggc aaacgcgccg taaatcgagc ggtagccgtc gaaattgccc atataccaag
      901 tgaagaggga gcgcgcggtt tccagacaaa acgctgttgc caaagccccg acaaacgcct
      961 gccgcgcggg aacgaagcgg tttggcacga agcggtacag cccccacagc aaaagcgtca
     1021 tgaaggtcag cgtcgccgcc gttcgcaacg cgcccgacca ctgcggcgca cctgaggcaa
     1081 gcgcggcatc ctgtaccgag ccgaccataa aggaaatgcc cacgcccaaa gacagcggcc
     1141 cgaacgtcag taaagcccaa tagacgagaa actgcatcat ccacggacgc tgggaattga
     1201 cccgccagat gcggttgaac gtattgtcta tcgtccgaat cagcatcagc gaggtaacga
     1261 ccagcatcat gctgccgatt gccgtcagcc ggttcgcctg ctcgcggaac gcattgatat
     1321 agtcgaacac catgtccgcg ccctgcggca caatggtttg gttgacgaag gagacgaacg
     1381 aatccgacca gcggtcgaac acggggaaaa tcgaagcgac cgccaccatc acggtcagca
     1441 cggggacgag tgccagcagc gtcgtaaacg tcatgcttgc cgccgcctgc ggtacgcgtt
     1501 cttcatcaaa gcggcggacg acgaaccatg caaacgcaca gattttattg tctgccaaac
     1561 cttgcaaacg ttgtaaaaag gtcataattt cttgcccggt cagtaagttg ggcattgatg
     1621 cccgatgtta tagccaattt tgccgtcagg aacaaatgcc tgaactgcgg ctgtttcaga
     1681 cggcatcgga acaactgtta tgccgtctga agaccgaacc attttaacgg aatccgccca
     1741 tgaacccaaa tccccctcaa aatcctcgtc ctctactatt tccaaaacgg cagcacccgc
     1801 aatcccgccc gccgaatcgc tcgcggcatc gacagcgttg aaggttgcga agccgtattg
     1861 cgcaccgtcc ccaaagtctc cgccgtctgc gaagccgtca aaaaagatat tcccgacagc
     1921 ggctcccgtc ctgaccgccg aagaaaacaa tatcgccttc gcacaaagca aacgcttggc
     1981 ggaactcgcc atcaagtcgg cataagccgc gtgttcagac ggcatggcgt tcagatgccg
     2041 tctgaacgcg tttgcctgta taatccgcgt ctttactgtc caacttcgcg gttcgcaaac
     2101 ctcccgcgtt accaaaacta ggattcgata tgtcaaacca aaaagccttg gtcatctttt
     2161 cgggcggtca ggattcgacc acctgcctga ttcaggcaat ccaaatctac gggcgcgaaa
     2221 acgtccaagc cattactttc caatacgggc aacgccatgc cgtcgagctg gaacgtgccc
     2281 gctggattgc gcaggatttg ggcgtcaaac aaaccgtact cgacttgagc ctgatgcggc
     2341 agattacgca caatgccctg atggacgaca ccgccgccat cgaaactgcc gaaaacggcg
     2401 ttccgaatac ctttgtagac ggccgcaacg cgcttttcct gctttacgcc gcgatttacg
     2461 ccaaagggca ggggatacgg cacatcatcg cgggcgtgtg cgaaaccgac ttctccggct
     2521 atcccgactg ccgcgacgtg tttgtcaaat cgatgaacgt tacccttaat ttggcgatgg
     2581 actatgattt tcaaatccac acgccgctga tgtatctgac caaggcgcaa acgtgggcgt
     2641 tggcggacga aatgggcgcg ctggactata tccgcgagca aacccacacc tgctacaagg
     2701 gcatcgtcgg cggctgccgc gaatgcccga gctgtatctt gcgcgaacgc gggctggcgg
     2761 aatatctgga aagtaaaaag gccgtctgaa cacgcgcaaa ccataaggaa tacgatatgc
     2821 ccaagctcca tatgttttac ctcggcggca atgccggcag gtcgaatatc gaagtgcacg
     2881 acatccaatt tgccgtgtgc gacgactacc gtgaagccgt ccccacactc aaagccgcat
     2941 ggttcggcga tgctgacaaa atccacatcg acggctggca ggttgtcgaa tgggcggacg
     3001 gttacgacat cgccgtatcc gaaacgccca aaacggaaat gccgtctgaa cacgccccgc
     3061 gcctgtattt tgccaatgtc ggcggttatc gcgcggggca gcttgccgag gcgcacgcgt
     3121 tcgggctgtt cgccgccgcc acgcctgccg aagccaaaca aaaagccctg caaaccctgt
     3181 tgaccgacag ctatgttcag caacataaag acaacttaaa agacgtggac aacctgcttg
     3241 cgctcgaccg catcggcaat ttccatatcc gcctgacccc gaatccgcac ggcaaacccg
     3301 ccgaaatcgg ctttcaaggc tatttgccca tttgagaacc catgaaaatc accaaaatct
     3361 tcaccttcga ctcctcgcat atgctcgacg ggcatgacgg caaatgccaa aacctgcacg
     3421 ggcataccta caaactcgaa atcaccgttt cagacgaccc cgtcagaggc gggccgaaag
     3481 acggtatggt gatggacttt accgacttga aagccattgt caaacaacac attaccgacc
     3541 ccttcgacca cgccttcatc taccacggcg gcaacgggcg cgaatctcaa atcgccgcac
     3601 tcttggaagg ctggaacatg aaaaccctgc gcctgccctg ccgcaccact gccgaaaata
     3661 tggcggtcga aatgtacgac cgtctgaaaa acgcggggct gaacgtgtgc cgcgtcaaat
     3721 tgtgggaaac gccgacatcg tgtgcggagt atgaagggga gtagggaata tcttgaacgt
     3781 atcgatatag taaattccaa taagacatgc ccaaccgcgt cattcccgcg caggcgggaa
     3841 tccagacctt ggtttttcag gaatatttaa aaattgcagc aattccaaat ctttggattc
     3901 ccgcctgcgc ggaaaggacg gtttagagcg tccttatttg aatttaccgt aaaacggttt
     3961 tttctcctgt acggattccc cgttttttca gacgaccctc catatcaaat acacccatta
     4021 aaaggaatac ccatgaaact tctctccacc ctcttagtcc tccttgtcac cgccgaacat
     4081 ttctacatcg cctggcttga aatgacgcag attcccagcg aaaaagcggc ggaaatattc
     4141 aagctgcctt atgaatttat ggaacaaaag cgcgtgcaga ccctgttcgg caaccaaggg
     4201 ctgtataacg gctttctcgg catcgggctg gtgtggtcgc ggtttgccgc tccggataac
     4261 gcggtgtacg gcgcaacggt actgtttctc ggcttcgtcc tgattgccgc cgcgtggggc
     4321 gcgttctctt ccggcaacaa aggcatactc gtcaaacaag gtttgcccgc atttttggca
     4381 gcggcggcgg tgttggcggt atgaaaaaaa tcaatgttgc ccccgaaaat ccccaatacc
     4441 gcatcgtcga aatctttgaa agcctgcaag gcgaaggctg gaacacgggc atgcccgccg
     4501 ttttcgtccg cttgggcaaa tgcaacctcg cctgcggctg gtgtgatacc gattatttga
     4561 cattcggcat gatgagcctg tccgacatct taggctgtct gaaaacctat gccgcccgca
     4621 acatcatcat caccggcggc gaaccgacca tacagccgca tctcgatatg ctgctggaca
     4681 tgctcaaggc ggaaggttat ttcctctgta tcgaaaccaa cggactcaat cccgcgccgc
     4741 cgcaaatcga ctatgtcgcc accagcccca aagcctgcta tgccgccaaa tacgaaaaaa
     4801 gctgtatcga aacagccgac gaagtgcgga ttgttgccga tggtgatgtc cttgcgttct
     4861 gtgaaaatat ggaacgcaaa atccgcgcgc atcattacta cctttcgccc tgcgaacaag
     4921 acggtgcgat gaacatctac gacaccatcc gccaaatcgg tattttaaac agtcgccccg
     4981 acgcgccggt gcattggcag ttgagcgtgc agacgcacaa atgggcggga atagagtagt
     5041 ttaagcagtg taactcaaag ggacggcgta cggttttacc gatgtttgac atacagggaa
     5101 agtgtgccgc ttctgcgtgg aaatgccggc atttccaccg cccaatcagg acggagcctt
     5161 actgaataag atgctgccgt tgggtacaag ctcggcttcc taaattccga tggtcttttg
     5221 aaccttgccg ataccctttg tcagtgcacg caaatagaag ggttagggaa aacgaaatgc
     5281 cgtctgaaac agcat
//
LOCUS       DAXHRI010000140         5283 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXHRI010000140 DAXHRI010000000
VERSION     DAXHRI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.140"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            113..808
                     /gene="pgl"
                     /locus_tag="ACFM75_001736"
     CDS             113..808
                     /gene="pgl"
                     /locus_tag="ACFM75_001736"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC6256242.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            789..1775
                     /locus_tag="ACFM75_001737"
     CDS             789..1775
                     /locus_tag="ACFM75_001737"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC6256243.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGTSPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1825..2673
                     /gene="hexR"
                     /locus_tag="ACFM75_001738"
     CDS             1825..2673
                     /gene="hexR"
                     /locus_tag="ACFM75_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC6256244.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2794..4440
                     /gene="pgi"
                     /locus_tag="ACFM75_001739"
     CDS             2794..4440
                     /gene="pgi"
                     /locus_tag="ACFM75_001739"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6256245.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELACEAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4454..5005
                     /locus_tag="ACFM75_001740"
     CDS             4454..5005
                     /locus_tag="ACFM75_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFC6256246.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKVKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEVRLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         1342 a         1467 c         1398 g         1076 t
ORIGIN      
        1 aaagcagtcc cgatatttgg tatcaaaagc ataaacaact gttcggccgg catattgctc
       61 aacccgtttc cataacggaa tatgccgtct gaaataaaaa aaggacacaa atatgtttgt
      121 ttggcacgaa tacgaaaatg cggcagaagc ggcgcagtct ttggctgacg cagtggcgga
      181 tgctttgcag ggcgcactgg acgagaaggg cggtgcggtg ttggcagttt ccggcggacg
      241 ttcgccgatt gcatttttca acgccctgtc gcaaaaagat ttggattgga aaaacgtcgg
      301 catcaccttg gcagatgaac gcatcgtgcc gaccgtccac gccgacagca ataccggttt
      361 ggtgcgcgaa tacctgttga agaacaaagc ggaagcggca atgtggattc ctatggtgga
      421 agacggaaaa actgaaaccg aattacatcc cgatgctgtt gtcgattatg cactgaaaca
      481 ttacaaacag cccgatgttt tggttttggg tatgggaaac gacgggcata cggcttcgat
      541 tttcccgaaa gctccgcagt ttcagacggc aatcgacggt tcggcaggtg tcgcgctggt
      601 gcataccacg cccgttaccg cgccgcacga gcgcgtcagt atgaccttgg atgcgattgc
      661 ccatacgggg catgtgtttt tggcgatacg gggcgaagag aaaaaagccg tgttcgacca
      721 agccgcacaa ggcgaaaacc gcgaatatcc gatcaacctc gttttgaacc atcaaggagt
      781 gaactgccat gtcttctacg ccgaataaac aagccggata tccccgactg gtcgccgata
      841 tcggcgggac gaatgcacgc tttgcgctgg aaaccgcgcc gcgcgtcatt gaaaaagccg
      901 ccgtgcttcc gtgtaaagac tacgatacgg ttaccgatgc ggtgcgtgcc tatctgaatc
      961 aaagcggtgc aacagccgta cggcacgcgg catttgccat cgccaacccg attttgggcg
     1021 actgggtgca gatgaccaac caccattggg cgttttccat cgaaaccacc cgtcagactt
     1081 tggggctgga caccctcatc cttttgaacg actttaccgc gcaggcattg gcggtaacgc
     1141 agacttcaag caaagacctg atgcaggtag gcgggcaaaa gcctgtcgaa tttgccccca
     1201 aagccgttat cggccccggt accggcctgg gcgtgagcgg attggtgcac agccacgcag
     1261 gctgggtggc tttggcgggc gagggcgggc ataccagttt cccgccgttt gacgatatgg
     1321 aagtgctgat ttggcagtac gccaaaaaca aatacggcca tgtttccgcc gaacgctttt
     1381 tgagcggagc gggcttgagc ttggtttacg aggctttggc tgcaaaacag aaagccaaac
     1441 ccgccaaact gatgccgtct gaaatcacgg aaaaggcttt gagcggcacg tcgcctttgt
     1501 gccgtcagac tttggacatc ttctgcgcca tgctcggcac ggttgcttcc aacctcgccc
     1561 tgacgctggg cgcgcgcggc ggcgtgtacc tgtgcggcgg cattattccc cgcgtgttgg
     1621 aatacttcaa aacttccccg ttccgcagcc gtttcgagaa caagggcagg tttgaagcat
     1681 atcttgccgc gattcccgtg tatgtcgtct tgagcgagtt tcccggaatt tccggtgcgg
     1741 ctgcggctct tgacaaccat ttgagaaacg tttaaccaca gcggctcctt gcagcggggc
     1801 tgcattatcg aagggcatat cattatgtta agcaaaatca gcgaatcact ggcaaacctt
     1861 tccggtgcgg aacgcaaagt cgccgaatgt gcattggcgg aacccaaatg gttcgtccat
     1921 gccgccgttg ccgaaattgc cgaacgtgcg tccgtcagcc agccgaccgt catccgattc
     1981 tgccgcagct tgggttataa agggctgccc gagttcaagc tcgccttgtc cgccagcatc
     2041 ggtcatgagg gtatgcccta tgtccacgaa gaactcaacg ccgacgacga tatggcaagc
     2101 gtggtcgaga aagtgttggg caatgccgcc gcctcgctgt tgggcgaacg ccgcttcctg
     2161 aaagagtcgg agctggaaaa cgccattgcc acgctgatgc acgcccgtcg cgtcgagttt
     2221 tacggtgtcg gcaattccgg cattgtggca caggacgcgc agcataaatt tttccgtttc
     2281 ggcatgtcca ccgtcgccta tgtcgatacg cacacgcagc tgatggcggc atctgttttg
     2341 agcgatcagg atgttttggt tgccatttcc aacacgggtt cgtctatcga acttttggat
     2401 gcggtcagca tcgccaaaga aaacggcgcg tctgtcatcg cactgacccg caacgattcg
     2461 cctctggcgc aacttgccga ctgcgtgttg agcgttgcca cacaggaaaa tgccgaactc
     2521 tacacgccca tggtttcccg cctcttgcag cttgccgtca tcgacattct tgccatcgga
     2581 cttgccctgc gcttgggcga tgctgccagc ctgcaattgc agaaaagcaa aaaaagcata
     2641 cacaacaagc acatcgatta cgacaaagat tgaccttcag acggcatccc acaaatgccg
     2701 tctgaaatgc cgaacaacgg ccgtcggcgg cttgcggcag tttccagcag ccttttcatc
     2761 ccacaacaaa aaacctcatt caggagcata tagatgaaac actttcacga cttacccgca
     2821 tggtcgaaat tgtggaacca ctttgacgac agcaaaacat tgcatatgcg cgaaatgttc
     2881 gagcaagacc cgcagcgtgc ggaacgctac tggctgcaag tcggcggact gacgctggac
     2941 tactccaaaa accgcatcaa cgacgaaacc atgtcgcttt tgttcgagtt ggcgtgtgaa
     3001 gcaggcgtgc cggagcggat gcggcagatg ttccacggcg aaaaaatcaa taccaccgaa
     3061 aaccgcgccg tcctgcatgt cgcccttcgc aaccgcacca attcgccgat tgtggttgac
     3121 ggtgaagatg tgatgcccaa agtcaaccgc gttttgcaac gtatgggcga atttgcacac
     3181 gaagtccgca gcggaagctg gctgggctat accaaccaag tcattaccga cgttgtcaac
     3241 atcggcatcg gcggatcgga tttgggcccg ctgatgatgt gtaccgcgct caaacctttc
     3301 ggtcatccgc gcctcaatat gcacttcgtc tccaacgtgg acggctcgca actgcgcgac
     3361 gtattgtcca aagtccaccc cgaaaccacg ctgttcatca tcgcctccaa aacatttacc
     3421 acgcaggaaa cgctgaccaa cgcgctgacc gcgcgcgaat ggtttttgaa tcatgcgggc
     3481 gacgaagaag ccgttgccaa acacttcgcc gccgtttcca ccaatcaaaa agccgtcgcc
     3541 gaattcggca tcgacaccgc caatatgttt gaattttggg attgggtcgg cggtcggtac
     3601 agcctgtggt ccgccatcgg attgccgatt atgctgtatc tcggcgaaga aaacttcatt
     3661 gaaatgctca acggcgcgca cctgatggac caacacttca tcaacacacc gctcgagcgc
     3721 aacctgcccg tcattctcgc cctcatcggc atctggtata tcaactacta cggcggcggc
     3781 agccacgtca tcgcgcctta cgaccaacat ttgcaccgcc tgcccaaatt catccagcag
     3841 ctcgatatgg aaagtaacgg caaacaggtt acgttggacg gcaaagcagt cggacacgaa
     3901 acctcgccga ttatctgggg cgaaacgggc attaacggcc agcacgcctt tttccaactg
     3961 ctgcaccaag gcacgcacat tacccccatc gacctgattg cctcgcttga aaaacgcagc
     4021 aacctgcccg gacaccacga aatcctgctt gccaacgtct tcgcccaagc agaagccttt
     4081 atgcgcggca aaacccccga cgaagtccgc gccgaactca aagcgcaggg tatggatgag
     4141 gcgcgcatcg aagagctggt cccgcacaaa accttctccg gcaaccgccc gaccaacctc
     4201 attctcatgg acaaggtcaa cccgcgcaat atgggcagcc tgattgccat gtacgaacac
     4261 aaaaccttcg tacaaggcat catttggggc atcaacagct tcgaccagtg gggcgtggaa
     4321 ctcggcaaac aactggctaa aaccattttg ggcgaactga ccggcgaaac cgggccgcaa
     4381 aagcacgaca gttcgaccga acgcctgatc aacctctacc tgcagaccaa ccgcaaataa
     4441 aacctgcgga aaaatgccgt ctgaacgccg accgtttcaa acggcatttt tatcgaacag
     4501 gaaaaccgtc ggtaaacttg cagagcgtgt gcaatcccga tatgatggtt tgcataaatt
     4561 taaacatata tgttccgcag ctatggcact gattaaagag ccgttggaca aagtgaaaca
     4621 aaggaacgaa gaacttgaag cggcagaaga agcggcggcg caggaggcat tgggtcggga
     4681 gcaggaagcc gcccgcgtat ccgaatggga agaacgctac aagctgtcgc gcagcgagtt
     4741 cgagcagttc tggaaaggat tgcctcaaac cgtacagaat aagctgcaag cctcacagaa
     4801 aacatggaaa agcgggatgg ataaaatctg tgccaacaat gcgaaagctg aaggtaaaac
     4861 gccaaacggc ataaaattca gcgaactggc atgcaaaacg gcgaaaaccg aagtacgctt
     4921 ggaagagctg cacaaccgta aaaaagccct tatcgacgaa atggccaggg aagcggacaa
     4981 gaaagaactg tcaaagcggc tctgaacagc gcggttcagg cactgcccgc cgatattgcc
     5041 gaaaccgtca tgcccgagta ccgcaactga caaaacggtt tgaatgccaa atgcgccgac
     5101 ggcgacgaat acggcgtggc acaatctgac tgccgtacca gagaaatcaa tgcgaaaacc
     5161 aaagaaatcc aaggttatct gattgactga aacttggatg cgggaatgtc ggcggctttt
     5221 gcgtttttgt cgtttttata gtgaattaaa tttaaaccga tacagcgttg gctcgcctta
     5281 gct
//
LOCUS       DAXHRI010000141         5249 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXHRI010000141 DAXHRI010000000
VERSION     DAXHRI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.141"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            345..716
                     /locus_tag="ACFM75_001741"
     CDS             345..716
                     /locus_tag="ACFM75_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256247.1"
                     /translation="MNITLGRNHQINCDEKDLYKFIGYLANHPNDVNLVFEKNSVQGA
                     WGDEGRIQFFSSKAQNIFVPLGFKFTAGVGNIAYRLNCNELFEMLSQLGFVSGGKQNL
                     STIKANIPSQFHAEFDAGANM"
     gene            complement(780..1940)
                     /locus_tag="ACFM75_001742"
     CDS             complement(780..1940)
                     /locus_tag="ACFM75_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6256248.1"
                     /translation="MNMENYFSNLNLDIRTHKLGTFTDQKVTPDVLCAVAECIIEYIE
                     NIGEIFSINDIRYSDYAEYIATAVFKKPSIENAGSEYNKFFSQPIKMLSYCGVLSEEK
                     FSRYYRYGVQNNKILQYIANRERNALNFIQAFSEKLLKDSGIYPKFADFFAQPNKNTF
                     ESMKTAFTDLVIQNTPKNTEVEVRRIFTKIINPLAYKHNTFGTRKGSISNTPITLDEL
                     YYNRLNWRDKGKEKSLTRKEAQALFADSANAANLNYLVNKAKKFVKMLHKTSEVQRFD
                     PTEANQAHHIFMASEFPDLASLPENLICLTPNQHFNLAHPSNKTTVIDKHYQRICLMA
                     KLDSIEQDNRANTGNYDYHEFIHVLNTGFNTDQFDVSMSYETLKHRILMFYF"
     gene            complement(1952..4540)
                     /gene="dcm"
                     /locus_tag="ACFM75_001743"
     CDS             complement(1952..4540)
                     /gene="dcm"
                     /locus_tag="ACFM75_001743"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC6256249.1"
                     /translation="MKNQYSKSLHGVFYLAFYDKIHAFFAEQLFQLIINMNKKPTYIS
                     LFSSAGVGCYGFKTNGFECVATNELLEKRLKIQKYNQKCRFDSGYIAGDITLPETQAK
                     LFAEIRRWNTEIDVVIATPPCQGMSVANHKKNNEMARNSLVVESIKIVREVQPKFFIF
                     ENVKAFLNTPCTDTDHQEKPIEEAIWSNLGGHYNILSNVLNFKDYGANSSRTRTLVIG
                     VRKDIHHITPYDIFPKQTPPKTLRTLLADLPPLMQMGEISDDDIYHSYREFDPRMLPW
                     IAQLKEGESAFQNTDPARIPHQIKNGEIIYNQNKNGDKYARWFLDREGPCIHTRNDIL
                     ASQNTIHPTENRVFSVRELMRMMSIPPSFKWTAQDEKQLNALSFDEKKTFLKKEELNI
                     RHCIGESVPTAVLANVAQNIQAALQQSELSMTEIQNLIKRENLSLADNLYRFIEQHFD
                     AYPFSRLLQIAEYANAARSETAAYFTRIDIAFGLVNALPDFKKKKNLRILEPSVGIGN
                     FIPLLAKKYADKEKVIFDLVDIDSNSLNLLSLLLKKLNLGSKFEFNIIHQDFLTATLP
                     ENYDLVIGNPPYGKVKASDKNLALYRKNATNKDTQNIFSFFIDKALTLSKTVALVVPK
                     SVINAPEFQITRQQLITANINRIIDYGEKAFKGVKIETIAFIADQTDKDNQHITIESH
                     IQETLIQNCKNYFFSDNFPYWLIYRNSEFDQIAQKLEFNVFQSFRDRQITKAHTQNSG
                     NVRVLKSRNIGNNEIIDIEGYDSYVDSVAPFSVGKFINRKNVVMIPNLTYKPRAAFLP
                     ENSIADGSVALLTMKKHEKILTQRDLAYYATPEFEQFYRVARNYGSRSMNIDNHSVFF
                     FGLLKD"
     gene            4703..4873
                     /locus_tag="ACFM75_001744"
                     /pseudo
     CDS             4703..4873
                     /locus_tag="ACFM75_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4951..>5249
                     /locus_tag="ACFM75_001745"
     CDS             4951..>5249
                     /locus_tag="ACFM75_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256250.1"
                     /translation="MTQYLPFFSLSIIRYAQITATQADTVPIAGKKCCQSVIGGTGQR
                     PHSISAPPNNRYSNISAIQSNNGYFKRCNQKTNHVRTIHLTPHSDNDIVPLPCNV"
BASE COUNT         1538 a          986 c         1049 g         1676 t
ORIGIN      
        1 gccaacaggt taagtgcgcc tgttgccgcc taaaaagcag cccggatgcc tgattatcgg
       61 gtatccgggg attaaggggg tatttgggta gaattaggcg gtatttgggg cgaaaataga
      121 cgaaaacctg tgtttgggtt tcggctgtca gcgaaaaggt gaagagaggt actttgcaaa
      181 ggtctcagcc tgaatatgtc aaccaaatca gcgacaagct cggagaattt tttggccgga
      241 cgttgaaata ggagtttcaa ttcttttaat tcagcaatag atactgataa aatgaaaatc
      301 ttgaggaata tatcattaaa attaatttgt taaggagata attaatgaat attacattag
      361 gtcgaaatca tcaaatcaat tgtgatgaaa aggaccttta caagtttatc ggctatttgg
      421 caaatcatcc taatgatgta aatttagtct ttgagaaaaa tagtgtgcaa ggtgcttggg
      481 gtgatgaggg acgtatacag tttttctcaa gtaaagcaca aaatattttt gtgccactag
      541 gatttaaatt tacggcaggc gtaggtaata ttgcatatag gctaaattgt aatgagcttt
      601 ttgaaatgct atctcagtta ggttttgttt ctggtggaaa acaaaatctt tcaacaataa
      661 aagcaaatat tccgtctcaa tttcatgcag aatttgatgc tggtgcgaat atgtaaattc
      721 taatttaaaa cacgaatccg cttcatgaga ttcgtgtttt ttatttttaa aatatttatt
      781 taaaaataaa acattaaaat acgatgcttc aatgtttcgt aactcataga aacatcaaac
      841 tgatctgtat taaatcctgt attgagtaca tgaataaatt catggtaatc gtaattgcct
      901 gtattagcac ggttatcttg ctcaatacta tccagtttcg ccatcaggca aatacgctgg
      961 taatgtttgt caataacggt ggttttattg cttgggtgtg ccaaattgaa atgctggttt
     1021 ggtgtcaggc aaatcagatt ttcaggcagc gatgctaaat caggaaattc gcttgccata
     1081 aaaatatgat gtgcctgatt ggcttctgtg ggatcaaaac gttgaacttc gctggttttg
     1141 tgtagcattt tgacaaattt tttggcttta ttaactaaat aattcaaatt tgcggcattt
     1201 gcactatcgg caaatagagc ttgagcttct ttgcgagtga gcgatttttc tttgccttta
     1261 tctcgccagt ttaggcgatt gtaataaagc tcgtctaatg taatgggggt attggaaata
     1321 ctcccttttc ttgtgccaaa agtattatgt ttataagcca atggattaat gattttggta
     1381 aaaatacggc gaacttctac ttcggtattt ttgggtgtgt tttggataac aagatcggta
     1441 aacgccgttt tcatactttc aaacgtattt ttattaggct gggcaaaaaa atcggcaaat
     1501 tttggataaa taccactgtc ttttaataat ttttcactaa atgcttggat gaaatttagg
     1561 gcatttcttt cacgattggc aatatattgc aggattttat tattctgtac gccgtaccga
     1621 tagtatctag aaaacttttc ttcgcttaat acgccacaat agctcagcat tttaatcggt
     1681 tgtgaaaaaa atttattgta ttcgctaccg gcattttcaa tggatggttt tttaaatacg
     1741 gcggtggcga tatattcggc ataatctgaa taacgaatat cgttaatgct aaatatttct
     1801 ccaatatttt caatatattc gatgatacat tctgctacgg cacataatac atccggggtt
     1861 actttttgat cggtaaatgt gccgagttta tgtgttctga tgtctaaatt taagttagaa
     1921 aaataatttt ccatattcat tttttactcc tctaatcttt tagcaatccg aaaaaaaaca
     1981 cagaatgatt gtctatattc attgaacgag aaccataatt acgggcaaca cggtaaaact
     2041 gctcaaattc gggagttgca tagtatgcga gatctcgttg ggttaggatt ttttcgtgtt
     2101 ttttcattgt cagtagggca accgagccat cagcaatact gttttcaggc aaaaatgctg
     2161 cccgaggttt gtaagtcaga ttgggaatca tcacgacatt tttacggtta ataaatttgc
     2221 cgacagaaaa aggggcaact gaatccacat aactatcata accctcaata tcaataatct
     2281 cgttgttacc gatattacgg gattttaaaa cccgaacatt gccactattc tgtgtatgcg
     2341 ctttggtaat ttggcgatct cggaatgatt gaaatacatt aaattccaat ttttgtgcaa
     2401 tttgatcaaa ttcactatta cgataaatca accaataggg aaaattatca gaaaaaaaat
     2461 agtttttaca gttttgtatc aacgtttctt ggatatggct ttctatcgta atatgttgat
     2521 tgtctttgtc ggtttgatcg gcaataaagg caatcgtctc tatttttacg cctttaaatg
     2581 ctttctcgcc gtaatcaata atgcggttga tattggctgt gataagttgt tgtcgggtta
     2641 tttgaaactc gggggcgtta atcacacttt tgggaacaac caaagccacg gttttactga
     2701 gcgtcagagc cttatcaata aaaaatgaaa aaatattttg cgtatcttta ttggtagcat
     2761 ttttacggta tagtgccaaa tttttatcac ttgccttaac tttgccgtat ggtggattgc
     2821 cgatgactag atcatagttt tcaggcagcg ttgcggttaa aaaatcctga tgaataatat
     2881 tgaactcaaa tttactgcct aaatttaatt ttttcagcag caatgacagg agatttaaac
     2941 tgtttgagtc aatatccacc aaatcaaaaa taactttttc tttgtctgca tatttttttg
     3001 ccagtagtgg aatgaaatta ccaatgccga cagagggttc taaaatccgt aagttttttt
     3061 tctttttgaa atcaggaaga gcattaacca gtccgaaagc aatatcaata cgggtaaaat
     3121 aagcagccgt ttcgctacgg gcagcattag cgtattcagc aatttgcaaa agccgactga
     3181 atggatatgc gtcaaaatgt tgctcaataa atcggtagag attatcggca agggataaat
     3241 tttctcgttt gattaaattt tgaatttccg tcattgacag ttcggactgt tgcaaggctg
     3301 cctgaatgtt ttgtgcgaca ttggccaaaa cagcggttgg cacagattca ccgatacaat
     3361 ggcggatatt cagctcttct tttttcaaaa aggttttttt ctcatcaaat gacagtgcat
     3421 taagctgttt ttcatcttgt gccgtccatt taaatgatgg cggaattgac atcatacgca
     3481 tgagttcacg cacggaaaat acacggtttt cagtggggtg gatggtgttt tggctggcta
     3541 aaatgtcatt acgggtatga atgcacggac cctcacgatc taaaaaccaa cgggcgtatt
     3601 tgtctccatt tttgttttga ttgtagataa tttcaccatt tttaatttga tggggaatgc
     3661 gtgcaggatc agtattttgg aaagctgact cgccttcttt aagctgtgca atccacggca
     3721 acatacgggg atcaaattca cgatagctat ggtaaatatc atcatcgcta atttcgccca
     3781 tttgcattaa aggcggcagg tcagccagca atgtacgcaa ggtttttggc ggtgtttgct
     3841 tggggaaaat atcgtacggc gtaatgtgat gaatatcttt tcgcacgcca atcaccagcg
     3901 ttctggtacg actggaattt gcaccataat ctttaaaatt tagtacattg gatagaatgt
     3961 tgtaatgtcc gcccaaattt gaccaaatgg cttcctcaat cggcttttct tgatgatcgg
     4021 tatcggtgca gggtgtattt aaaaaggctt tgacgttttc aaaaataaaa aacttgggct
     4081 gaacttcccg aacgattttg attgattcca cgaccaaaga atttcttgcc atttcattat
     4141 tttttttatg gttggcaacc gacattcctt ggcaaggcgg tgtagcaatc acaacatcaa
     4201 tttcagtatt ccagcgtcga atttcggcaa acagtttggc ttgggtttca ggcagggtaa
     4261 tatcgccagc aatataacct gaatcaaagc ggcatttttg attatatttt tggattttta
     4321 ggcgtttttc taaaagttcg ttggttgcta cgcattcaaa accattggtt ttaaaaccat
     4381 agcaaccaac gcctgcactg ctaaataaac tgatgtaggt gggttttttg ttcatattta
     4441 tgatgagttg aaacaactgc tctgcaaaaa atgcgtgtat tttatcatag aaggcaaggt
     4501 aaaacacgcc atgcaatgat ttactatatt gatttttcat caaaaaagtg ctgtttgaac
     4561 ggatatacag cggtgttttt tgcaatgcgt ttcaaacttg aggtacgttt ttaatagaaa
     4621 caccgtctga acgccaacct gagacctttg caaaattccc ccaaatcccc caattcccac
     4681 caagacattt aggggatttc tcatgagcac cttcttccgg caaaccgccc aagccatgat
     4741 tgccaaacac atcgaccgct tcccgctatt gaagttggat caagtgattg attggcagcc
     4801 gatcgaacaa tacctgaatc gtcaaaaaac ccgttacctt cgagaccacc gcggctatcc
     4861 cgcctatcct gtcgccggta ttccctaagc ggtacggcaa cctaaaacaa atgccgtctg
     4921 aacttccgtt tcagacggca ttgcgtccgg atgactcaat atcttccgtt tttttcactt
     4981 tcaataatca gatatgccca aatcaccgcc acccaagccg acaccgttcc catcgccggc
     5041 aaaaaatgct gccaatccgt catcggcggc acagggcaac ggccgcattc gatttccgcg
     5101 ccgcccaaca accggtacag caacatatcc gcaatccaaa gcaacaacgg gtatttcaaa
     5161 cgctgcaacc aaaaaaccaa ccacgtccgc acgattcacc tcacccccca ttccgacaac
     5221 gatattgtac cgcttccgtg caatgttaa
//
LOCUS       DAXHRI010000142         5246 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXHRI010000142 DAXHRI010000000
VERSION     DAXHRI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.142"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(201..1244)
                     /gene="argC"
                     /locus_tag="ACFM75_001746"
     CDS             complement(201..1244)
                     /gene="argC"
                     /locus_tag="ACFM75_001746"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC6256251.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLVPMIRGMHATVYLHLSDGICPETVLRDYYRDSPFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(1241..1534)
                     /locus_tag="ACFM75_001747"
     CDS             complement(1241..1534)
                     /locus_tag="ACFM75_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256252.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            1788..2942
                     /locus_tag="ACFM75_001748"
     CDS             1788..2942
                     /locus_tag="ACFM75_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6256253.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            2951..3157
                     /locus_tag="ACFM75_001749"
     CDS             2951..3157
                     /locus_tag="ACFM75_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256254.1"
                     /translation="MKNWKQFTFFVILVIACYQLLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQ"
     gene            3362..3622
                     /locus_tag="ACFM75_001750"
     CDS             3362..3622
                     /locus_tag="ACFM75_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6256255.1"
                     /translation="MKRKNNIIELSIEDLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSSGIPAGPAIILGGTVGAAVGAVTGMGVAAYEYY"
     gene            3917..4498
                     /locus_tag="ACFM75_001751"
     CDS             3917..4498
                     /locus_tag="ACFM75_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFC6256256.1"
                     /translation="MIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVE
                     DGEKVKAGDKLFALSTSRFGAGDSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSL
                     KATVERLENQKLHISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLE
                     QKAKLDAYRREEVGLLQEIRTQNLTLASLPQAA"
     gene            4563..5057
                     /locus_tag="ACFM75_001752"
     CDS             4563..5057
                     /locus_tag="ACFM75_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6256257.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYEWVLELIYSM
                     SGKL"
BASE COUNT         1420 a         1161 c         1329 g         1336 t
ORIGIN      
        1 aatcgaagcg tgtggaaatg tgtcggaaat tgctgaagcc ggataaacta gattcccgct
       61 tttgcgggaa tgacgggatt ttaggttttt gttttcgtgg gaatggatgg gcccaaagcc
      121 gtccgaacca aaaaaaaccg ccgctttcag acggcatcaa aaaaccgcag gcacgatgcc
      181 tgcggtttgc cgtttgcgct tcaggggagc agggggattg cgtctaagcc gtgtgtttcc
      241 ttcagtccga acatgatgtt catattttgg actgcctgac ccgccgcgcc tttgacgagg
      301 ttgtcgatga cggaaaggac gacccacaca tcggattgcg ccgcctgttg gatgctgatg
      361 cggcagaggt ttgcgccgcg cacgctgcgg gtttcgggtg tcgaaccggc aggcaggatg
      421 tccacgaacg ggctgtcgcg gtagtagtcg cgcaggacgg tttcggggca gatgccgtct
      481 gaaaggtgga ggtaaacggt ggcgtgcata ccgcgtatca tcggcacgag gtgcggcgtg
      541 aacacgaatc cttcggcgat gccgtcctga agcccggcga tggtctgcct gatttcgggc
      601 aggtggcggt gtccagctat gccgtaggct ttgaagttgt cgccgacttc gcacaacagc
      661 gaaccgacat tgcctttcct gcccgcgccg gacacgccgg atttgcagtc ggcaatcagc
      721 ggcataccgg gcttcagacg gcattgccgc aacagcggca cgagcggcag ggatacgcag
      781 gtcgggtagc agccggggtt ggcgacgagg cgcgcctgtg cgacggcttc gcggttgagt
      841 tcgctcaatc cgtacacggc ttgggaaacg aggccggggg cggcgtgggt catgccgtac
      901 cagtgttccc aggtcggaat gtcccgtatg cggaagtcgg cggaaaggtc gatgacgcgc
      961 acgccctgtt caatcaggcg cggcgcgtct ttcatggcga tgccgttggg cgtggcgaag
     1021 aagacgatgt cgcattgttc caaacctgcc tcgtcgggcg tttggaaggc gaggccgtac
     1081 acgccgcgca aactcggaaa gtaatcggca actgcagttc ccgcttcgct gcggctggtt
     1141 acggcggcga cttcgacatc gggatgggcg gcaagcaggc gcagcagttc cacgcccgtg
     1201 tagcccgtcg cgccgacaat gccgactttg atttttttac tcatggtttt tcctttgtgt
     1261 ggttggcggg tatgccgtcc gaacgctgtc cgacggcttg tccgacgggg ttttgattgg
     1321 cggcggaagg acggtacaca aagcgtttga aaagcgtgtc taatttaatg gcatccgcct
     1381 ctgtttttaa aacccagccc tgtcgtccgg aataaacata accgtaggcg cgaaagtcgg
     1441 caggatattt cggtgcctgc cccaaaccca aaccgtatgc ggcgaaagcg gtggttgtgg
     1501 agaaagaaag caggaggagg acgagggttt tcataaggtt ccaaaccgaa cggggcgatg
     1561 aagcccgatt atagcaaaag gtcgtctgaa aaccgttttc agaagacctt ttatttaatt
     1621 acaataaaaa tgttaacaac aaaaaacaaa ccgctttttt ccgtttggag acaaattttt
     1681 aactaaaatt gtcaacaatt ccttgacgca cacacaaaaa aaactttaat atttgtaaat
     1741 atatgtaaca ttataaattt taaatatttt ctgagtaagg ggaagtaatg agcatccatc
     1801 tcgacttcgg cattagtcct aaaacgttcc gacagactta tctgtatcaa aagcccaagc
     1861 tctttaaagg agcggttcgg aatctcgaat ccgcatcttg gaaagatatc aacgagatat
     1921 accaacgagc agacccaacc gcaccgctgt ttcatctgcg taaaaaaggc gcaatcgttc
     1981 ctaaagaaga atacgtcgaa agtttcgacg atttgggcaa aactcgctac cgttttatta
     2041 aatccgttat ctacgaacat atgaagaatg gtgcgtcgtt agtctataac catattaaca
     2101 acgagccgtt ttcagaccat atcgcccgtc aagtcgcccg ctttgccggc gcacatacta
     2161 ttgttagtgg atatcttgct tttggcagcg acgaatctta taaaaaccat tgggacaccc
     2221 gcgatgtgta tgccatccag cttttcggca agaaacgttg gcaacttact gcccctgatt
     2281 tccctatgcc attgtatatg caacagacta aagatactga tatttccatt cctgaacata
     2341 tcgatatgga tattatcctt gaagcaggtg atgtcctcta catcccacgc ggttggtggc
     2401 acagacctat cccgctcggc tgtgaaacct tccacttcgc tgtcggtacc ttcccgccca
     2461 acggctataa ttacctcgag tggctaatga agaaattccc cacgatagaa agtctgcgcc
     2521 acagtttctc agactgggag caagatagga cgcgtatcaa cgatactgcc gcacaaattg
     2581 ctgccatgat tgccgacccc gtcaattacg aagccttcag tgaagacttc ctcggcaaag
     2641 aacgcaccga taccgctttt catctcgaac agttcgcgaa tcccaacgct actccgcttt
     2701 cagacgacgt caggttgaga ctaaatgcca ataatttgga tacgttggaa aagggatatt
     2761 tgattgggaa tgggatgaag ataagcgtag atgaattggg gaaaaaagtg ttagaacaca
     2821 tcggtaagaa tgaaccgtta ttgttgaaaa atctactggt taacttcaat cagggaaaac
     2881 atgaagaagt taggaagttg atttatcagt tgatagagtt agattttctg gaacttttgt
     2941 gagggattct atgaaaaact ggaagcaatt tacatttttc gtaatattag taatagcttg
     3001 ttatcaattg ctatattttt tatcagatat gtttcttctc gactacatta acaaatatag
     3061 ttggaatttg aattttattc agggtacttt gaattttttt tcaatatatc tgccatatgt
     3121 ttttgttagc cgaattttta ggaataccaa ccaatgaaag actaggctat gaaatcaaaa
     3181 tttgtgtata agttactggt gcttgcaaag tacctgtata ttttaatttg tatatcaata
     3241 ccaatcgttt ttatttatga aagctaccaa ggagatgatg ttaattttat gatttatatt
     3301 cgtgctttta ttattttttc catattgggt gtattgactc aatattctat gtctaatttt
     3361 tttgaaaagg aaaaataaca tcattgaatt atccattgag gatctggagc taatttatgg
     3421 cggaggctta ggggagagaa tccttgaagg aggagcaaat ggtgcacaaa taggaggatc
     3481 aattggtgca ggtattggtc tgggtgtcgg tatagtttct agtggtattc ctgccggacc
     3541 ggctattatt ctcggtggca cagttggtgc tgctgttggt gctgtaactg gtatgggtgt
     3601 tgcagcatat gagtattact aagaaactca gcccaaaggg acagtaacgg tcggtccgat
     3661 ttctgctttt gctgtggaca aatcaggaaa cggctacact gacagcacaa attactgctg
     3721 atatttctca tcgtccagac aatctctagg ggtcgtctga aactttctta acttcaattt
     3781 tatgaataga cccaagcaac ccttcttccg tcccgaagtc gccgttgccc gccaaaccag
     3841 cctgacgggt aaagtgattc tgacacgacc gttgtcattt tccctatgga cgacatttgc
     3901 atcgatatct gcgttattga ttatcctgtt tttgatattt ggtaactata cgcgaaagac
     3961 aacagtggag ggacaaattt tacctgcatc gggcgtaatc agggtgtatg caccggatac
     4021 ggggacaatt acagcgaaat tcgtggaaga tggagaaaag gttaaggctg gcgacaagct
     4081 atttgcgctt tcgacctcac gtttcggcgc aggagatagc gtgcagcagc agttgaaaac
     4141 ggaggcagtt ttgaagaaaa cgttggcaga acaggaactg ggtcgtctga agctgataca
     4201 cgggaatgaa acgcgcagcc ttaaagcaac tgtcgaacgt ttggaaaacc agaaactcca
     4261 tatttcgcaa cagatagacg gtcagaaaag gcgcattaga cttgcggaag aaatgttgca
     4321 gaaatatcgt ttcctatccg ccaatgatgc agtgccaaaa caagaaatga tgaatgtcaa
     4381 ggcagagctt ttagagcaga aagccaaact tgatgcctac cgccgagaag aagtcgggct
     4441 gcttcaggaa atccgcacgc agaatctgac attggccagc ctcccccaag cggcatgaga
     4501 cagaacaaag ctggcttgaa cgcaccattg ccgatatttc tcaagaagtt ttggattttg
     4561 aaatgcgctc tgaacaaatc atccgtgcag gacggtcggg ttatatagca ataccgaacg
     4621 tcgaagtcgg acagcaggtt gatccttcca aactgctctt gagcattgtt cccgaacgta
     4681 ccgagctata tgcccatcta tatatcccca gcagtgcagc aggctttatc aagccgaaag
     4741 acaaggttgt cctacgttat caggcatatc cctatcaaaa attcgggctt gcttccggca
     4801 gtgtcgtatc agtagcaaaa acggcactgg gcagacagga attgtcggga ttgggcatgg
     4861 tatcctccga tttggcgaag agcaacgaac ctgtttatct cgtgaaaata aaacccgaca
     4921 aaccaaccat cactgcatac ggtgaggaaa aaccgctgca aatcggcatg acgttggaag
     4981 cagacatcct gcacgagaaa cggcggctgt acgaatgggt attggagctg atttatagta
     5041 tgtcgggcaa actgtaagac ttgaggctga aagataaaga agttgtctga aaccttttca
     5101 gacgaccaga agacaataaa aaacacaaac ttcaaaactg cctgaaacca tcctctttta
     5161 caggcactcc gtctgtaaaa gcaaggatag ggcatctgcc cttttgattg tttgattaac
     5221 gatacaagga gtttcaaaat gagagt
//
LOCUS       DAXHRI010000143         5201 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXHRI010000143 DAXHRI010000000
VERSION     DAXHRI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.143"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            210..1394
                     /gene="ubiM"
                     /locus_tag="ACFM75_001753"
     CDS             210..1394
                     /gene="ubiM"
                     /locus_tag="ACFM75_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC6256258.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1665..1820)
                     /gene="rpmG"
                     /locus_tag="ACFM75_001754"
     CDS             complement(1665..1820)
                     /gene="rpmG"
                     /locus_tag="ACFM75_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC6256259.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1852..2085)
                     /gene="rpmB"
                     /locus_tag="ACFM75_001755"
     CDS             complement(1852..2085)
                     /gene="rpmB"
                     /locus_tag="ACFM75_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC6256260.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2335..3861)
                     /gene="farB"
                     /locus_tag="ACFM75_001756"
     CDS             complement(2335..3861)
                     /gene="farB"
                     /locus_tag="ACFM75_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC6256261.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(3885..5039)
                     /gene="farA"
                     /locus_tag="ACFM75_001757"
     CDS             complement(3885..5039)
                     /gene="farA"
                     /locus_tag="ACFM75_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC6256262.1"
                     /translation="MDTHADETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         1324 a         1459 c         1249 g         1169 t
ORIGIN      
        1 gtttaaattt aatccactat acatacccca cacccgattt gatacacatt aagctgaaag
       61 taaaaatccg catacaccct cccctgcata tttcttcaac agtgggtttt gatataatcg
      121 cctatctgtt acagatagtt caaacggcat tcaaaccctt acaaataccg tctgaaatca
      181 tcggcatccg tctatgcaaa ggatatttta tgagtttaca cagcgacatc ctcgtcgtcg
      241 gcgcaggccc ggcgggttta agttttgcag ccgaacttgc cggcagcggt ttgaaagtta
      301 ccctgatcga acgcagccca ctgactgttc tgcaaaatcc tccatatgac ggacgcgaaa
      361 tcgccctgac ccatttttcc cgcgaaatca tgcagcgttt gggtatgtgg gacaaaattc
      421 ccgaaaacga aatctaccct ttgcgcgatg ccaaagtgct gaacggacgt tccgactacc
      481 agctccactt tccccaaccg actgaagcgc gcggcgaacc tgccgactgt ttgggctatc
      541 tgatttccaa ccacaatatc cgccgcgccg cctatgaagt cgtatcacaa ctcgacaatg
      601 tcagcattct gaccgatacg gttgtcaaag aagtcaaaac atctgacaac gaagcacaag
      661 tcattttgga aaacggcaaa atcctgactg cacgcctgct tttggcggca gacagccgtt
      721 tctcacaaac ccgccgacaa ctcggtatct cctccgatat gcacgattac agccgaacca
      781 tgttcgtctg ccgtatgaaa catacccttt ccaaccaaca taccgcttac gaatgcttcc
      841 attacggacg caccatcgca ctgctgccgc tggaagagca cctgaccaat accgtcatta
      901 ccgtcgatac cgataaaatc aacagcgttc aaaacctttc gcccgaagaa ttggcggcaa
      961 gcgtaaaaga acaactcaag ggtaggcttg gcgacatgga actggtcagc agcatccacc
     1021 attaccccct tgtcggtatg attgccaaac gtttctacgg caaacgcagc gcgctgatcg
     1081 gcgatgcggc tgtcggtatg caccccgtta ccgcacacgg tttcaacttg ggtctgtcca
     1141 gcgcagatat tttggcaaaa ctgatactcg aagccgagca gcgcggtcag gacatcggcg
     1201 cggcttcctt gttggaaaaa tacagcagca aacatatgct gcacgcccat ccgctttatc
     1261 acgggaccaa tatgatgctg aagctgttta ccaacgaaac cgcaccggca aaactgctgc
     1321 gcggtttggt attgcgtgcc ggcaacaact tcccgccgct gaaaaagctg attaccaaac
     1381 aactgaccgg ttgaaaactg ccgaaatgcc gtctgaaacg tttcagacgg catttttgta
     1441 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc tgcagacagt acaaatagta
     1501 cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctatc cttggcagac
     1561 ggtttgcagc agattcgcat acggcagatg tttcaagcgg aaaggaaaca cgataaaaat
     1621 aacaaagcct ccgagcagtc ggaggctttg ctttcaaact gaaattattt cagtttggtt
     1681 tctttataca ctacgtgttt gcgggcaacc ggatcgaatt ttttaatttc cagtttgccg
     1741 ggcatagtgc gtttgttttt ggtagtggtg tagaagtgac cagtacctgc actggattcc
     1801 agtttgattt tatcgcgcat tgcagtaatc cttaattaaa tagtgtttaa attaagcttc
     1861 gccgcgagca cgcaaatcag ccaatacgac atcaatgcct actttgtcga tggtacgcag
     1921 tgcagcgttg gaaacgcgca ggcgaaccca gcggttttca ctttctaccc aaaaacgacg
     1981 tgattgcaag ttgggcaaaa aacggcgttt ggttttgttg ttggcgtgcg atacgttgtt
     2041 gccggacatc gggcgtttgc cggtcacttt gcaaactcgt gccataatca gtctccaaac
     2101 ttctaaaata gtaaaacgcg atttatacca cgaaaaacag tgttagttca agtgttttag
     2161 agaaaaacgg ctgttttacg cggataatcc ttccgattgt cgcatctgtg gaacgtttgc
     2221 aggtgcgaaa cagatggggc gcgattatag cggatttcgg gaactgctgc attcaaaatg
     2281 ccgtccgaac atatttccag tattttcaga cggcatttca agttttcaaa tccctcaatg
     2341 tccaccgccg ccgccgttgt ggaacggcgg ttttgccagc catatgacgg gtatcatgat
     2401 aatgaataac aagctgcccg ccataaagat ttcgttcgag ccgataataa aaccctgctg
     2461 ggtaatggta ttgttgatga tgcctagggt ttgaatgtcg gaaacgccgt gctgggacaa
     2521 atgagcggcc gtttcgtgca atgttgcgga atagggcgtg atgtgttcgg caaagcgtgt
     2581 atggtgcaac gcttcgcgcc gttcccacag ggtgctgacg acggatacgc cgacaccgcc
     2641 catcagcacg cgcaagaaat tcgacaggct gcctgcggcg gcaatctgtc cgcccttcat
     2701 gtgcgacagt gtgatggtgg tcagcggcag gaagaacatg gcaacgcctg ccccctgcca
     2761 aaactgcggc cagatgacgt tgccgatatc catgtcggca taaaaatcgg tgcgccaata
     2821 gaaagtaaag gcaaaggtca ggaagctggc ggttacgagc aggcgcatat cgattttatt
     2881 gccgaacctg ccgattaacg gagacaggaa gacgggcagg atgccgacgg gtgcggcggc
     2941 aagccccgcc cacgtggagg tatagcccag gttggtctgc aacactaacg gcagcagggt
     3001 cagcgtcccc atatacacca taaaacccaa tgatgtggca attacgccga cggtaaaatt
     3061 ccgatcttta aacagcgata aatcgacaat cggatatttt tctcccaatt cccaaacaat
     3121 aaaatacgac aaacacacca gtgcgactac gcccaaggta atgatttctc cagaggcgaa
     3181 ccagtcgagt tccttacccc tgtccagcat catctgtaac gcgccgatac cgactaccat
     3241 caatgtaagc ccgacatagt cggtcggcat tttaacggtt tccgtttccc gatatttcaa
     3301 atgtttccat gtaatccatg ccgatatgat accgataggg atattaatga agaaaatcca
     3361 accccaatgc cagtttccgg aaatccaacc gccgagtatc ggcccgagaa caggggcgac
     3421 aacgacggtc attgcccaca atgccagtgc cagcgtccgt tttgcgggcg gataggatgc
     3481 cattaacagg ctttgcgaca ggggaatcag cggcccggcg ataaagccct gcaagatgcg
     3541 gaaaacaacc agcgactgaa ggttgggcgc gatgccgcac agccacgatg tgatgacgaa
     3601 accgacagcg gcggcggtaa acaatttgac ctcgccgata cgttttgcca aaaagcccgt
     3661 taacggcacg gaaacggcgt ttgccacagc aaaagaagta atgacccacg tcccctgcgt
     3721 ggttgccgca ccgaggttgc cggcgatgac gggaacggcg acattggcga tagtcgtatc
     3781 taaaacttcc ataaatacgg caagccccaa agacagcgta acccacgcca atgccgcacc
     3841 cttaagcggt ggataatcca ttgtgtcctc cgtttcagac ggcatcaggg cgcggattgc
     3901 ccgaggattt catcgaccgt ccgatcgact tccgaccaat cggtactttc catttccggc
     3961 aatgccgcac ccggcgtttt tgaaacaggc gcgcctgcgg cggaagtatc cactttaacc
     4021 gtcatcgaca aaccgatacg caacggatgc ctgtccacat cttcgcggtt cagcacgata
     4081 cggacgggga cgcgctgcac cactttaatc cagttgcccg ttgcgttttg cgccggaatc
     4141 agcgaaaacg cgctgcccgt acctgccgaa aaccctgcca ccctgccgcg ataaacaatt
     4201 tgtttgccgt acaaatcgga caccagctcg gcaggctgtc cgattttcat atgccgcaac
     4261 tgcgtctctt taaaattggc atccacccac acatccgaca gcggcaccac cgccatcagc
     4321 ggcgcgcctg ccgccacctg ctgcccgacc tgcaccgaac gcttcgccac ctgaccgtcc
     4381 gccggcgcgc ggatttgcgt ccgctgaagg ttcaaccacg catctttcaa cctgccgatt
     4441 gccgtctgaa ccgccggctg ttcgcgcaaa gaaacctgac cgcccaaagc cgcgcgtgcc
     4501 gaagattctt ccgccaaagc cgctttgacc gccgcctgcg cctgagacac tgccgcacgg
     4561 gcgtgtgcca gctcttcggc ggacaccgcg cccgattccg ccaaagcaga ccggcggcgc
     4621 aaatcatcct gcgcgcgtgc caaatccgca cggcgcaagg caacctgcgc ccccgcctgc
     4681 gaagtggcgg cattttgccg gcggttttgc cgcaccgcct gaatcagctc gttttttgcc
     4741 cgctcgtaag ccagcacatc attatcgtcg tccaataccg ccagcacgtc gccttttttc
     4801 acggcatccg tatcgtcgtg caaaaccttc cgcaccgtac cgcccttttg cggcgtaacc
     4861 tgaaccacgc gcccagcaac ataagcgtct tccgtttctt cttcgtgctg ccaccataaa
     4921 aaaaacgccg acccggcggc tgcggcggca agcgcgaaca gcagcgtcaa tgccgtcagg
     4981 cggcgtttgc gtttggcttg cgtgttttga agttttgttt cgtccgcgtg cgtatccata
     5041 agattgggtt ccgaatttcc tgatttcata taaacaatgt tctttcaata ggaagcagat
     5101 tctatatccg cgcaataacg caatcaagtc agtattttag atgcaatatt caaaaaacaa
     5161 cgcttcaggc ggcaaaactg aaaatatatt tcgtttatat a
//
LOCUS       DAXHRI010000144         5165 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXHRI010000144 DAXHRI010000000
VERSION     DAXHRI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.144"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(193..1548)
                     /locus_tag="ACFM75_001758"
     CDS             complement(193..1548)
                     /locus_tag="ACFM75_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC6256263.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGCEASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1563..1835)
                     /locus_tag="ACFM75_001759"
     CDS             complement(1563..1835)
                     /locus_tag="ACFM75_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC6256264.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1961..2698)
                     /locus_tag="ACFM75_001760"
     CDS             complement(1961..2698)
                     /locus_tag="ACFM75_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC6256265.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVVAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2979..3878)
                     /gene="prmB"
                     /locus_tag="ACFM75_001761"
     CDS             complement(2979..3878)
                     /gene="prmB"
                     /locus_tag="ACFM75_001761"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6256266.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            4023..4616
                     /locus_tag="ACFM75_001762"
     CDS             4023..4616
                     /locus_tag="ACFM75_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256267.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHATSKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDGHKHYDHFILKDE"
     gene            4728..>5165
                     /locus_tag="ACFM75_001763"
     CDS             4728..>5165
                     /locus_tag="ACFM75_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC6256268.1"
                     /translation="MTKLYAEIAKREAQDDGTVKVWGYASSEAVDSDGEVIATAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAVSTCFKADK"
BASE COUNT         1420 a         1318 c         1144 g         1283 t
ORIGIN      
        1 agttcaatga gtttattttg tttatactgg cttagacgac tttttctcat agggataatt
       61 ctaacttaat ttgaatttcc ctagttatct aggacagccc catattcttt tctaaagaca
      121 ttttacccga tgcctataaa caaaaatgcc gtctgaagcc tttcagacgg catttcccca
      181 tcaaaaccgc aatcagtttt tcatcgattg aaccggtgcg ggaatcctgc cgccccggtt
      241 gacgaacact tcgcacgaac cttctttgac cggcattaca ggcgcgtagc ccaacaggcc
      301 gccgaactcg acgctgtcgc cgacggtttt acccgttacc ggaataatgc gcacggcagt
      361 ggttttgctg ttaatcatac cgatggcggc ttcgtcggca atgatgccag aaatggtgtg
      421 cgcgggtgtg tcgccgggaa cggcaatcat atccaagccg accgaacaaa cggcggtcat
      481 cgcttcgagt ttatccaacg tcagcacgcc tgcttcggcg gcggcaatca taccttcgtc
      541 ttcggaaacg gggataaacg cgccactcaa accgccaacc gcgctcgaag ccatcatgcc
      601 gccctttttc acggcatcgt tcagcaatgc caaagctgct gttgtgccgt gcgtaccgca
      661 gacgctcaaa cccatttctt caagaatgcg cgccaccgag tcgccgacgg caggggtcgg
      721 tgccagcgac aagtcgagaa taccaaacgg gatattcagc atttttgagg cttcgcagcc
      781 gatgagttcg cccacgcggg taattttgaa ggcggttttc ttcacaactt cggcaacttc
      841 ggtcaatgtc gtcgcatccg aattttccaa cgcggctttg accacgcccg gaccggatac
      901 gccgacatta atcacagcat ccgcttcgcc cgatccgtga aacgcacccg ccataaacgg
      961 attgtcttcc accgcattac agaacacgac gattttcgcg caaccgaaac cttcaggcgt
     1021 gatttcagcc gtgcgtttga tggtttcgcc tgccagcttg accgcatcca tattgatacc
     1081 ggcacgcgtg ctgccgatat tgatggagct gcacacgatg tcggtggtct tcatcgcttc
     1141 gggaatggaa cggattaaca cctcatctga aggcgacatg cctttttgca ccaacgcgga
     1201 aaagccgccg ataaaggaca cgccgatggc tttggcagcc ttatccaaag tttgcgccac
     1261 gctgacatag gaatcggcat gggtagccgc cgcgatttgg gcaatcggcg tgacagaaat
     1321 gcgctgattc acaatcggca cgccgtattt ggcagacaga tattttgccg tcgccaccaa
     1381 gtctttgccg accgtggtga ttttgttgta aatgttttgg tttaacacgt cgatgtcggt
     1441 gctgatgcag tcgtgcaaat caatgccgat ggtaatggtg cggacatcga aattctggtc
     1501 ggcaaccatt ttgacggttt ctaaaatttc gccggattgg atactcatca cattcctccg
     1561 gctcaaatgc ggtgcatcgc ttggaagatt tcttcgtttt gcatacggat gtcgagcgcg
     1621 agttttttgc tctcttccgc aaacaaatcc aaaacctcct gccgcgattt gctgcatttt
     1681 gaagtgtcca ccaagataat catagtaaaa aaatcgtcca tcagctgttg gctgatgttg
     1741 agaatattga tttggttttc cgccaaaatt ttggaaacat cgtacacgat gccgacgcgg
     1801 tctttaccga tgacggtgat gactgaattg ttcacaggct tactccttgc agatatccgt
     1861 taaagtccga aattatacca ccgttggatt ttgaagaaat attgtcaaca atatatacat
     1921 acaaaatgcc gtctgaaact atttcagaca gcatcaagat tcagggttcg attaaataac
     1981 catccttatc ccactgggtt ttcctgacca acttgtcatc ctgataaaca gcttcgctct
     2041 ttttagaacc atcttcatac cactccaaaa ccaccccgtt gcgttgatgg tggcggatag
     2101 acagttccga gagtaatcgg ccgctttcat cccaagtcag aattttggca ggctcatcgt
     2161 tgaccataac catttccgtc ttgatactgc cgtcggcata ccattgcttc catacgccgt
     2221 ttgccttatt ttgcttaaac tggatttcgc tttccttgcc gccgttacgg taatagcggt
     2281 atcccgtacc ctcactcaag ccatttttat aaggcataac ggcagatttt ttaccgttcg
     2341 gataccagtt gacccactcc ccgtccggct tacccttgct gaagcccccc gccatttttt
     2401 tctgaccatt aaaatgccac aaaatcaaca taccgttttg cagggtaggc acaaaagatt
     2461 tgatttgcgt tgaagcaacg atataaggtt cagaatattt cttcatcgac ggataataaa
     2521 aatcctgcgc gtgcgcaata cccgccacca cactatattg cctgatataa gcggcagaag
     2581 acatcgtcgc cgtcagcttt ccgttctgat taaaataaac agaataggtc tgcgccggca
     2641 aagcggccga aaaacccaac aggacagttg aaaatacaat ccgagataat tttttcattg
     2701 caatagcgat ataaaaacaa ggctgtgttt tagtaatctg ttgatttcaa ttatttgcaa
     2761 gggaaaagac aattattttc cggttaggaa taaacctatt ctattgaata tattgaagcc
     2821 aagtacgcct atcaacacta tattaaaaca ctgccaaaaa aaattaactt ataaacaata
     2881 tggtaaggat ttctctgcca agcatcaaac ccgagacaac gtatcgtaaa aatgccgtct
     2941 gaaaacaaat cgtcttcaga cggcatttcc ccttcaactc actcttcacc caataactgc
     3001 tcgcgcgtca agaggaaaac aaaaccgtcg cccccgctgg tttccaacca agtaaaaggc
     3061 aactccggat acgctgcttc caatacatcc ctgttatgcc cgatttccac cagcaataca
     3121 cctttgggat tcagaaactt tgccgcattc agaagaatct gcctggtggc atccaacccg
     3181 tccgccccgc tgcccaatgc caattccggt tcgtgcaaat actcttcagg caataactca
     3241 accgattccg catccacata aggaggattg gaaacaatca aatcataagt gccttccaat
     3301 ccttcaaaca aatccgtatg aataagccgg atgcgttctt ccaaaccata atcttcgaca
     3361 ttaatccctg ccacttccaa agcatccaag ctcacatcaa ccgcatcaat ttgggcatca
     3421 ggataatgat gcgccatctg aatggcaagg caaccgcttc cggtgcaaag atccaaagca
     3481 ttatgcacca actcatcgta ttctatccaa ggacgaagtc cgtcacccaa caattcataa
     3541 ataaaagaac gaggtatgat tacgcgctca tccacataga aatcaaactc tccctgccat
     3601 gcctggtgtg tcaaataagc ggccggaatg tgttcgacag cacgacgctc aataaccgcc
     3661 agcacttcct ctttttcagc ttccaagagt tttgcatcaa gatatggggc aagcatatcc
     3721 aaaggcaaat tcaaagtatg cagaatcaaa taagctgctt catcatgcgc attatctgtt
     3781 ccatgaccaa aaaagagccc tgcctcatta aaacggctga ctgcaaaacg taaaatatcg
     3841 cggatagtcg tcaattcttg tgctgcctga ttaaacataa tatgaaccat tctgcgtata
     3901 gatactttta attataacag aaacaacaag caaacctttt catatcgcca aataaccacc
     3961 caatctaccc atacaactac ataaatgccc gcgcgaaaac catcgcccga acggaaacga
     4021 caatggccga cggtatgggc aatctgattg gctgggaaaa aacggggctt gttgtcggta
     4081 agcagtggat aaccgcaaaa gacgacaagg tgtccgatgt ctgcaatgcc aacggcgaga
     4141 tgggcgtaat cgggctttac gagcctttct cacacggcgc attgacgata cccggtcatc
     4201 cgaactgccg atgcgaggtt gtttccgtat cgggtggcga attgggggaa tttgccgaaa
     4261 aaaaggagct tcgtaaagcg gctatgcagt atgcgcggga taactttatc ggcaaaagct
     4321 atgtcaatga aaacagcggg catgaactga aggtaacttg gcaaggtgtg aaacacgcta
     4381 cgtcaaaggc aaatcaggcg gaattatcca tcatgacaaa acttgatgac ttattgcgct
     4441 acgcaaaata tgagggttct tattcggata ggaaaggtca tcctaatatt attgcagcac
     4501 ataagtatcg tgccgttgcc aaggttggga atgagtcttt aaatatcggg gtgattgtaa
     4561 gggaatttcc agacggacat aaacattacg accatttcat cttgaaggat gagtaaagcc
     4621 cttttgcagt gtcgttctgg agcggatagc gttaaggcaa gtacacttcc agccttgaaa
     4681 aagggcttta aattcagcat gccatttata caggcaggag taaacccatg acaaagttat
     4741 acgcagaaat cgccaagagg gaggcgcagg acgacggcac ggtcaaggtt tggggttatg
     4801 cctcaagcga ggcggtcgat tcggacggtg aagtcatcgc gacggcagct atgaaggcgg
     4861 cgattcccga ttatatgaag tttgacgcgg ggcgcgagat gcacggctca aacgctgcgg
     4921 gaacggcaat tgaaatcaac gttgaagacg acggcataac ctttttcggt gctcatatca
     4981 tcgaccccgt tgtcgtgtcg aaagtcaaaa caggcgttta caaaggcttt tccatcggcg
     5041 gcagcgttac cgcccgcgat gatttgaaca agtcgcaaat cacgggcttg aagctgacgg
     5101 aaatcagcct tgtcgagcgc cctgccaatc ccgatgcggt gtctacctgc tttaaggcgg
     5161 acaaa
//
LOCUS       DAXHRI010000145         5150 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXHRI010000145 DAXHRI010000000
VERSION     DAXHRI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.145"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(59..1315)
                     /locus_tag="ACFM75_001764"
     CDS             complement(59..1315)
                     /locus_tag="ACFM75_001764"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC6256269.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCVELLQNLKIDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(1663..2004)
                     /locus_tag="ACFM75_001765"
     CDS             complement(1663..2004)
                     /locus_tag="ACFM75_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256270.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2208..3065)
                     /locus_tag="ACFM75_001766"
     CDS             complement(2208..3065)
                     /locus_tag="ACFM75_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC6256271.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLTLDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVARLNGKPYHIELTCKYYGGGTGRPEDMSGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRDVYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3126..3941)
                     /locus_tag="ACFM75_001767"
     CDS             complement(3126..3941)
                     /locus_tag="ACFM75_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC6256272.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFHPDDVQSFVLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEAQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDMPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4005..4175
                     /locus_tag="ACFM75_001768"
     CDS             4005..4175
                     /locus_tag="ACFM75_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256273.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            4185..4970
                     /locus_tag="ACFM75_001769"
     CDS             4185..4970
                     /locus_tag="ACFM75_001769"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6256274.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            complement(4951..>5150)
                     /locus_tag="ACFM75_001770"
     CDS             complement(4951..>5150)
                     /locus_tag="ACFM75_001770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256275.1"
                     /translation="IVQGSPFNQIWVSIFGSFGHLAISTFLPPLPVKMPSEISARYFR
                     RHTRPFSRIPGSGLKFKRVRK"
BASE COUNT         1163 a         1207 c         1410 g         1370 t
ORIGIN      
        1 atgaaatgcc gtctgaacgc tttgaaaagg gttcggacgg cattttgttc aaaccggttt
       61 atttgcgttg caatacggcg gcgaagaagc cgtcggtttg gtgtcgggcg gaatcgaggc
      121 gcaggtattt tccggtatcc aaatcaatct tcaggttttg cagcagttcg acgcagttga
      181 cgggttcaaa ttcgggatgt tcggacagga aacgttcgac ttgcagctcg ttttcttcgg
      241 gcaggatgct gcaagtggcg taaaccaagc ggccttgcgg ttttaccagt ttggaggcag
      301 catcgaggat gctgtgttgc tgttccaaaa ggttggccac ggtctcggca gattggcggt
      361 atttgaggtc gggattgcgg cgtaaagtac ccaaaccgga gcagggcgcg tccaccaaca
      421 cacggtcggc tttgcctgcc agtcgggcga tacggctgtc gtgttcgctg ctgatgcgtt
      481 cggggtggat attggtcagt ccggcgcggg tcatacgcgg tttgaggttg gcaaggcgtt
      541 tttcggcgat gtcgaaggcg tagattctgc ctttattggc catttgcgca ccgacagcca
      601 aggttttgcc gccggcaccg gcacagaaat cgacaatgat ttcgccgcgt tttgcgccca
      661 ccaataaagc aagcagctgg ctgccttcgt cttgaacttc cagcgtaccg tccaaaaaca
      721 gctcgtgttt gttaagcgcg attttgtttt tcaggcggat gccccaaggc gaataaggcg
      781 ttgcctctgc atcggcactt tcggcttgca acaatggcag cactttatcg cgtttgcctt
      841 tcaaggtatt gacgcgtata tcgagcggcg cagcttggtt gatgctgcgg ccgaaggcga
      901 ggatttcttc ttcgcgccaa tgctgctgca gttgttcgac cagccattgc ggcaattctg
      961 cggcggtatt caggccgtct gaaaactcgg ttttacgggc tttcaaattg ccgagaaact
     1021 ctatttcttc ttcatcaagc aggtctttga tttggctgat gttggtgctt ctgccgagaa
     1081 ccagtgcggc gagtgcggct ttgcgcggct gcgcgtgcgg acggcgcagg gcggtactga
     1141 ttttttgata gtggcgcagc gcggcaaagg cggtttcggc gatttcgtgg cggtcttggc
     1201 ggccgagctt tttgtgttcg cggaaatagg cggagaggac ggcatcggaa ggctgtttga
     1261 aagtcagcat ttcagccaaa actttggcgg tatggtcgag ttgtgcggcg ttcataatgt
     1321 aatgggttct cttactgtgg gttattcata atcaatctaa cgtccttaaa ataacaatag
     1381 catctttatc attacttagt attctatccg caataagaaa gatgtgttaa aagtgcgcaa
     1441 tcgggatttg tctaaatttc tccgttaact acaaaggcag cctgaaaaaa gttctatttt
     1501 caggtcgctt gaacagcatc cgatataatc acatcagcga tttcattttg taatcactcc
     1561 ctaaaacatg aggaaaaaaa caaaatttat tttcttcgtt cagtatatct atcctaaata
     1621 tgccatcact ccattgagtt tttaatttgt atttcgctct tgttaaatgt ttttctgttt
     1681 gccgatttct tttgctaatg taaaaaatac tctaccactc tcattggtta aagttttcaa
     1741 gtattcatca acatcagtaa cattaataac aactgtttca tttgtatgtt tattaaattc
     1801 taaatcaata ataatattcc cattaaccaa tatttcatat atatcttctt cttcagggag
     1861 catagacaaa caataagcac ttgaagctga aaactctttt ttcagatact gaagaagaaa
     1921 agtattttta attaaaatat tattaaattt atctaatgat tgcttgactt ctttagttgt
     1981 ccaattttta acattattct tcattgtatt ctacctgggg tcgtagttat taaattacca
     2041 tacttatttg tttggatcgt cataatatta gttggcatgt tgttgttata tttatcatat
     2101 ccaatatcct tattaactcg gatgactctt tcataaattg tatgggaaat gagatttcca
     2161 tcactaagcc tctcaataga tatacgcgtt aatgttggga aagggtttca gacggccttc
     2221 ataatgcgtt cgatttcgtg ccaaaagccg tccggccgac attccaaaac ggcaatcaag
     2281 ccttggtcga tacggcggat ttcggttgcg ttcaatgttt cggtttcggc gacacgcgca
     2341 ggcgcgaggt aggccatacg gtcgagcaga tggtagcgga cttcttggcg tttaaacccg
     2401 tattccgccc aatcttgaat atagacgtcg cgccaaccgt atggattaag cggtggctca
     2461 aaagcatgga aaccgtgtgg aaaaaatccg atgccgcgca cgatggaaac gggttttacg
     2521 tcaagcggca gaccgtgttg ccgcaaggtt cggatgccgt ctgaagtgtg cggcagaccg
     2581 agttgggcgg tcagtttggc ggcttttccc aacagcgtgt ctttggggtc gagtccgctc
     2641 atgtcttcgg gtctgcccgt gccgccgccg taatatttgc aggtcagctc gatatggtag
     2701 ggtttgccgt taagccttgc cacaaaatcc gccgcgcctt gcgtattgcc gtccgaaccg
     2761 gaaaccgtga ggttgtgcgc gagcagttcg gcgtgtggcg cgttggcaaa ccaaaatgcc
     2821 agcagttctt cagcgtaaat gcccaagcgg tgtccgaacg gggcgcgtcg ggtgaggtgt
     2881 tccgtcagcg gcgcgggatc ggcatccaat gtcaaaaggt aacggaaacc gcgttccccc
     2941 agtagttcgc gcacgcccaa ttcgcaaccg ctttgccaca aaggcggcgc ggtcagcagc
     3001 gaggcgaggt cgcggacggg ttggctggtg agcttccacc ataatgcgtc tagggcgtaa
     3061 ttcatgctta tccgaatatc agtcatgccg tctgaaaagc tggaaatgtt ttcagacggc
     3121 cttaatcagt cttttttatg gatacgatgg ctgacggtat tgaaaaaacc gcgcaggata
     3181 tcgatgtctt cggtttgcgt gttggcgcgt ccgaacaggc tctgcatacg gcgcatcaga
     3241 cgctcgccgt tgcggcggtt gaaaaagccg atgtcgttca tcacgctttc catgtgggcg
     3301 accatgcctt tgatttgctc gtgggtcgcg gcgtggtctt cctgttgaag atgggtcatg
     3361 ggcatatcgg tctggctgaa gatttcgtag cacacgacct gcacggcttg ggcgaggttg
     3421 agcgagaaat agtcgggatt gccgttgatg gtcatcagtc ggttgcaggc ttgggcttct
     3481 tcgatgctca agccgaaagt ctcgttgccg aaaaccagcg ccactttctc gccgcggttg
     3541 gcggcctgta gtaattcggg caccaaatcg cgcggcgttt gcagcggcgc ggtaatttcg
     3601 cggcggcggc tggtcagggc gcaggcgatg gtcgtgtcgg caagggcttc gtccaaagaa
     3661 gcggcaatgg tggcattttc caaaacgtcc gccgcgcccg aagcgaggat gaaactttct
     3721 tcgggcagta caaaactttg cacgtcatcg ggatggaaaa cgggcgggtt ttccgtcatc
     3781 ggcgtagcca tcagattggg ggcgacgatg gtcagtttgt gcaggcccat tgttttcatc
     3841 gcgcgcgcgg cagagccgat gttggcggga tggctggtgc gcgtgaggat gatgcggatg
     3901 ttgtccagat aagcgggcag ggcgggtttg agagtagtca tgttcggttt tcggtcggaa
     3961 tggaataggg tataatgcgc cgtctttttc gttcggacgg tcaaatgccg tctgaaatgt
     4021 tctttaacaa cgtcggaaat ttaacgttgc gcctgaagca gacacgcgtc gggcggtatt
     4081 ttacccgatt tggcaggatt taccccaacc aaataaactc cgattttgcc gctccgaagg
     4141 actgcgcgtc cgaaccggcg gtatgccacc cttaaggaaa tctgatgaat ccgtttttga
     4201 atacagcctt taaagccgcc cgccgtgccg gtcagatgat gatacgcgcc gcgggcaatc
     4261 tcgatgccgt caaaaccgac agcaaagcat tcaacgattt tgtttccgat gttgaccgca
     4321 attccgaaat catcctggtt gaggctttga aagaagccta tccgcaccac aaaatcactt
     4381 gcgaagaaag cggctcccac ggtaaagccg cagccgaata cgaatggatt atcgatccgc
     4441 tcgacggcac gaccaacttc cttcacggcc atccccaata cgccatctct atggcactcc
     4501 tgcacaaagg cgtgttgcaa gaagctttgg tgtacgcccc cgaacgcaac gacgtttaca
     4561 tggcttcgcg cggcaaaggc gcgttgctca atgaccgccg catccgcgtt tccaaccgca
     4621 tcgaattgaa ccgctgcctg atcggtaccg gcttccccgt tgtcgatcaa agcatgatgg
     4681 acaagtatct ggcgattttg aaagatttct tggccaaaac cgccggcggc cgtcgtgaag
     4741 gcgcggcttc tttggatttg tgtgctgtag caacaggacg ttttgacggc tttttcgagt
     4801 ttaacctcaa accgtgggac attgccgccg gtgcattgat tgttcaggaa gcgggtggta
     4861 tcgtaaccga tatgacgggt gaagacggct ggctggaaag cggcgatatt gtggctgcca
     4921 atcccaaagt gctggcgcaa atgttgaaaa tcatttccgc acacgtttaa atttcagccc
     4981 gctcccggga atgcgggaaa acgggcgggt atgccgtctg aagtatcgcg ccgatatttc
     5041 agacggcatt tttactggca gcgggggcag gaaggtggaa atggctaaat gcccgaaact
     5101 gccgaagatg gaaacccata tctgattaaa cggagaaccc tgtacgattt
//
LOCUS       DAXHRI010000146         4928 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXHRI010000146 DAXHRI010000000
VERSION     DAXHRI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.146"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(276..1004)
                     /gene="rmpM"
                     /locus_tag="ACFM75_001771"
     CDS             complement(276..1004)
                     /gene="rmpM"
                     /locus_tag="ACFM75_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC6256276.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1368..2318
                     /locus_tag="ACFM75_001772"
     CDS             1368..2318
                     /locus_tag="ACFM75_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC6256277.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2431..3165)
                     /gene="fnr"
                     /locus_tag="ACFM75_001773"
     CDS             complement(2431..3165)
                     /gene="fnr"
                     /locus_tag="ACFM75_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC6256278.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3370..4791
                     /gene="hemN"
                     /locus_tag="ACFM75_001774"
     CDS             3370..4791
                     /gene="hemN"
                     /locus_tag="ACFM75_001774"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC6256279.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1256 a         1347 c         1195 g         1130 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tctgaatctg aatctgaatc tgaatctgaa
       61 tccgaatccg aatccgaatc cgaatccgaa tccgaatccg aacctgcacc ccgattctga
      121 cgatagccgt ttcagaaaac cacccaaaaa aacaaacccc gcaaaagcgg gggtttgttt
      181 cacatcatcg atgccgaccg acgtttcacg aatattgcaa cagtacctgg tacaaaatcc
      241 actaacctca tgcatcggca agatattgcc tagccttagt gttggtgatg attgtgtgcc
      301 ggcacaacct gacgggttac gatgctgcgg attttcacat ccacacggcg gtcaggttcg
      361 atacatgcaa tcagagcctc acgttttttg gctttagaga ctttcgcacc cagtttggca
      421 acttcggctt cacaaacttg agtcatttgc gcttgagatt cgcccaagcc aacagcagaa
      481 attctagaaa caggtacgcc gttgctgacc aggttgtttg ccactacgta tgcgcggcgt
      541 tcggacaggg cctgattgta tttgtcagaa cccataaagt cggtatggcc ttcgacgcgg
      601 acagattgga cattggttcg actcaggcgt tgcgccaata ctttcaggtt gtcttgagct
      661 tcggcgcgca atgaatcctt atcgaaaccg aacagggttt tggcagacag ggaaatggtt
      721 tcatcaacat attgcggagc ctgctccaca acgacgacag gcgcgggtgc gggttcgggt
      781 tctggctcgg gttcgggggc agcaaccgca tcgccgcatt ctacgcgacc ttggcttgct
      841 ttatcaaagt aggcgttttt ccagcattcg ccatagttgt tgcgtacgat ttcgttcgac
      901 tggccgctta cggtgtaacc ctgaacggac gcctcgcccg caacagcagt gccggaagcg
      961 agcaatgcaa cgaataatgc gcttaatttc agctgtttgg tcattttatt ccctcattaa
     1021 atttgtacag cagatacaga agcaactgct gaattgccaa catacaggcc gctgtagaaa
     1081 catggcggca aatcggatat ttcagtctcc actataaaga agcaggggga ggactgtcaa
     1141 tactcaacgc ctgaaaacaa ccgtaaaaca gcctgtttcc tgatagtcta tcatgccgaa
     1201 aaatcaattc cgtgtcatta ctttaggggg attttttcca tatcgcacga ttgccgttgc
     1261 acaaaaacaa caaacacaac ggcaaagccc cataccgtac cggcaagaaa atatgataaa
     1321 ttataaacaa tgttcagcca cccgacacag acccacaccg acccgccatg aaattacaac
     1381 aattgaaata cgccttagaa gtttaccagc acaacctgaa cgtttccgaa gcggccgaag
     1441 ccttgtttac ttcgcaaccc ggcatctcca aacaaatcaa attgctggaa gaagaaatcg
     1501 gcattcagat ttttatccgc agcggcaagc gcgtggtttc ggtctcgcag ccgggcaagg
     1561 tggttttgga tattgcggaa cgtattttac gcgatgtaca gaacattaaa aatatcggca
     1621 gcgagtttac cggacaggac agcggttcgc tgacggttgc cacgacgcat actcaggcac
     1681 gctatgccct acccttgatt gttgccgatt ttgtgaaacg ctatccgaaa gtcaacctga
     1741 ccatcaaaca ggggagccct gccgccatcg cacgtatggt tacctcaggc gaggcggact
     1801 tggcgattgt tacggaacgg atagacgacc atcccgaact gggaaaactt ccctgctatg
     1861 actggactca tgcggttatc gtaccgaacg accacccctt gctcgaatgc agaaaccccc
     1921 tccgcattga agatttggcg aggtttccgc tgattactta tgaatttgca ttcaatgcgg
     1981 gcagcagcat cgcgcgggca ttttccaaag cccgtttgga acagccggat gtcgcattgg
     2041 cggcggcgga tacggacgta ttgaagactt atgtgcgctt gggtttgggc gtgggactga
     2101 tggcgaaaat ggcgtacaac ccggatacgg acggcgattt gcagcttgtg gatgcggcac
     2161 acctgttcga gccgtcgccg acgtggattg ctttgcgcag cgatacttat ttgcgcggat
     2221 atgcctacga ctttatccaa gcgtttgcgc cgcacctgac acgcgagaag gtggatagga
     2281 ttctgtacac gcccatcagc gaggattttt cgatttaggc ggctgccggt tttcagacgg
     2341 cactttgcgg cagatacaac aaacaggaca gatgttttcg tctgccctgt gtttattgag
     2401 aatgctgtct gaaatgttcg tacgggttaa tcaaatggcg tgcgagcagc cggacaccat
     2461 ttttttcaac acctgcagat tgaggatttt gatgtgctta tgctcgacgg aaatcaatcc
     2521 ttcctgatgg aacttggata atgtgcggct gacggtttca agtttcagcc cgagataact
     2581 gccgatttct tcacgagaca tgcgcaggat gaaatcgttg gcagcaaaac cacgggaata
     2641 aaggcgttgg gagaggttca gcaggaaggc ggcaatccgc tcttcggcgc gcatattgcc
     2701 caacagcagc ataacacctt ggtcgcgcac gatttcacgg ctcatcatgc ggaagaagtg
     2761 cgtacgcagg ctggggatgt tttgccccag ttcttcgatg tgggtaaacg gcagttcgca
     2821 cacttcgctg tcttccaagg cgaccgcgtc gcaactgtgc acatgggaac agatgccgtc
     2881 catgccgatg agttcgcccg acataaagaa acccgttacc tgatcgcggc cgtcctgact
     2941 ggcgacggtt gttttgaaga agcccgaacg gatggcaaag agcgaggtaa aggcttcgcc
     3001 gacacagaac aggtattcgc cctttttcag gcggcggctt tgacggatga cggcatcgag
     3061 ttggctgagc tcgttgggca gcagcccgac aggcaggcag agttcccgca aagaacagga
     3121 agaacacagc gttttcatct gatgtgtagt attatgcgaa gccataccgt acctttttgt
     3181 gcgctttgcc ccatcatgat tatagtggat taaatttaaa ccagtacggc gttgcctcgc
     3241 cttgtcctta tttaaattta atccactata tgtgcttatt gacacatatc aagacaggtt
     3301 tatcatactg tggcattcta ccaaactatc caaaatttga ctaacatcat aaaatcgcac
     3361 acacacacca tgaaaatcat tcagatacag aacaatcaca atgtaaacga tgaccgcccc
     3421 gagtttgacc gcgcgctgat tgccagcctg cccgccagcg gcccgcgcta cacttcctac
     3481 cctaccgccg accgtttcca tgacggtttc cgcgaaggcg aatatatcaa agctttacat
     3541 ttgcgcggta tgggcgcgtt aaacaaaccg ctttcccttt acattcacat tccgttctgc
     3601 aacaccatct gctactactg cggctgcaac aaaatcatca ccaaagacaa aagccgcgcc
     3661 gatgcctaca tcgaatatct tgaaaaagaa atggaactgc tcgctccaca tctgaacgga
     3721 cggcaccagc ttgcccaact gcacttcggc ggcggcacgc cgaccttttt gagcgacgaa
     3781 cagatcgaac gtgtcttccg catgatacgc aaacatttcg agttaatccc caccggcgaa
     3841 tactccatcg aaatcgaccc gcgcaaagtc agccgcgaca ccgtcctcat gctcggcaga
     3901 ctcggcttca accgcatgag catcggcatt caggatttcg accccaaagt gcaggcggcg
     3961 gtcaaccgca tccaaagtta cgaagaaacc aaagaagtca tcgatgcggc gcgcgaagcg
     4021 gggttcaaat ccgtcagcgt cgatttgatt tacggcctgc cgcaccagac ttcggaaagc
     4081 atcaaaacca ccatcgatac cgttttgtcg ctcgatcccg accgcctcgc cctttatcac
     4141 tacgcccacc tgccgcacgt gttcaaaccg caacgccgca tcgataccgc cgccgttccc
     4201 gacagcgaag agaagctcga tatgctgcaa tactgcgtcc aaaccctaac cgaacgcggc
     4261 tacgtcttca tcggcatgga tcatttcgcc aaacctgacg acgaactctc catcgccctc
     4321 aaagaaggct tcctccagcg caacttccaa ggctattcga cctacgcgga ttgcgatttg
     4381 gtcgccatcg gcgtgtcgtc catcggcaaa atcggcagca cctattccca aaacgaacgc
     4441 gacatcgatg cctactatgc cgccatcgac gaaggcagac tgcccatcat gcgcggttat
     4501 cagctcaatc aggacgacat cctgcgccgc aacatcattc aggatttgat gtgccgtttc
     4561 gcgctcgact atcggattta cgaaagtatg ttcggcatcc cgttcgaccg ctacttcaaa
     4621 gacgaactgg cggatttgga aaaactcgcc ggtttgggat tggtgcgcct gaacagccac
     4681 gggctgaccg ttaccccgaa aggacgcttc ctcatccgca acatcgccat ggtcttcgat
     4741 taccacctgc gccataaaga aaccaaggcg aaatattcgc aaacggtgtg attgcgtcta
     4801 acgtacaaat gccgtctgaa aggctttttc agacggcatt ttgctgccgg caggataagt
     4861 gttttcaaga acaggcggcg gcatatcata acgttccgca cctttgtgtc cgaccgttcc
     4921 gaaaccaa
//
LOCUS       DAXHRI010000147         4833 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXHRI010000147 DAXHRI010000000
VERSION     DAXHRI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.147"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>4833
                     /locus_tag="ACFM75_001775"
     CDS             <1..>4833
                     /locus_tag="ACFM75_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6256280.1"
                     /translation="IAGHGLDARDTDFTQILSHAVKIDGPVWGKDVRVVAGQNDVVAT
                     GNAHSPILNNAAANTSNNTANNGTHIPLFAIDTGKLGGMYANKITLISTAEQAGIRNQ
                     GQLFASSGNVAIDANGRLVNSGTMAAANAKDTDNTAEHKVNIRSQGVENSGTAVSQQG
                     TQIHSQSIQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTDTLNNQGKLSQTG
                     SQKLHIDAQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASFHSSTTTPTTATGTGT
                     ATVSISNITAPTFADGTIRTHGALDNSGSIIANGQTDVSAQQGLNNAGQIDIHQLNAK
                     GSAFDNHNGTIISDAVHIQAGSLNNQNGNITTRQQLEIETDQLDNAHGKLLSAEIADL
                     AVSGSLNNQNGEIATNQQLIIHDGQQSTAVIDNTNGTIQSGRDVAIQAKSLSNNGTLA
                     ADNKLDIALQDDFYVERNIVAGNELSLSTRGSLKNSHTLQAGKRIRIKANNLDNAAQG
                     NIQSGGATDIGTQHNLTNRGLIDGQQTKIQAGQMNNIGTGRIYGDNIAIAATRLDNQD
                     ENGTGAAIAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNGQATGKAQRIHNAG
                     ATIEAAGKMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHELLREGTQHELGWS
                     VYNDESDHLRTPDGAAHENWHKYDYEKVTQKTQVTQTAPAKIISGNDLTIDGKEVFNT
                     DSQIIAGGNLLVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHKGRDSTGHSEQNY
                     TLPEEITRNISLGSFAYESHRKALSHHAPSQGTELPQSNGISLPYTSNSFTPLPSSSL
                     YIINPVNKGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLGDGYYEQRLINEQ
                     IAELTGHRRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQVAQLTSDIVWLV
                     QKEVKLPDGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSGSLKNSGTIAGRN
                     ALIINTDTLDNIGGRIHAQKSAVTATQDINNIGGILSVEQTLLLNAGNNINSQSTAAS
                     SQNTQGSSTYLDRMAGIYITGKEKGVLAAQAGKDINIIAGQISNQSEQGQTRLQAGRD
                     INLDTVQTSKHQATHFDADNHVIRGSTNEVGSSIQTKGDVTLLSGNNLNAKAAEVSSA
                     NGTLAVSAKNDINISAGINTTHVDDASKHTGRSGGGNKLIITDKAQSHNETAQSSTFE
                     GKQVVLQAGNDANILGSNVISDNGTRIQAGNHVRIGTTQTQSQSETYHQTQKSGLMSA
                     GIGFTIGSKTNTQENQSQSNEHTGSTVGSLKGDTTIVAGKHYEQIGSTVSSPEGNNII
                     HAQSIDIQAAHNKLNSNTTQTYEQKGLTVAFSSPVTDLAQQAIAVAQSSKQVGQSKNG
                     RVNAMAAANAGWQAYQTGKSAQNLANGTTNAKQVSISITYGEQQNRQTTQVQASQAQA
                     SQIQAGGKTTLIATGAAEQSNINIAGSDIAGKAGTILIADNDITLQSAEQSNTERSQN
                     KSTGWNAGAAVSFGQGGWSLGVTAGGNVGKGYGNGDSVTHRHSHIGDKGSQTLIQSGG
                     "
BASE COUNT         1615 a         1166 c         1067 g          985 t
ORIGIN      
        1 aatcgccgga cacggtttgg atgcccgtga taccgatttc acgcaaatat tgagccatgc
       61 ggttaagatt gatgggcctg tgtggggtaa agatgtgcgt gtcgtcgcgg gacaaaacga
      121 tgtggtcgca acaggtaatg cacattcgcc tattctcaat aatgctgctg ccaatacgtc
      181 aaacaataca gccaacaacg gcacacatat ccctttattt gcgattgata caggcaaatt
      241 aggaggtatg tatgccaaca aaatcacctt gatcagtact gccgagcaag caggcattcg
      301 taatcaaggg cagttgtttg cttcttccgg caatgtggcg attgatgcaa atggccgttt
      361 agtcaatagt ggcacgatgg ctgccgccaa tgcgaaagat acggataata cagcggaaca
      421 caaagtcaat atccgcagtc agggcgttga aaacagcggt acggcggtat cgcaacaagg
      481 cactcaaatt cacagtcagt cgattcaaaa cactggcaca ttattgtcct caggcgaaat
      541 attgattcac aattcgggca gcctgaaaaa tgaaacatca ggcaccattg aagccgctcg
      601 tttggctatt gataccgaca cacttaataa tcaaggcaaa ctctctcaaa caggttcaca
      661 aaaactccat attgatgcac aaggcaaaat ggataaccgt ggccgcatgg gtttacaaga
      721 taccgcacca accgcgtcaa atggttcaag caatcaaacc ggcaatagtt acaatgcatc
      781 tttccattca tccactacca caccaacaac ggcaacaggt acgggtactg caaccgtttc
      841 tatatcaaac ataactgcgc ctacctttgc tgatgggaca attcgcactc atggtgcact
      901 ggataattca ggcagtatta ttgccaatgg tcaaacagat gttagtgcgc aacaaggttt
      961 aaataatgca ggacaaatag acattcatca gttaaatgca aaaggttcgg cgtttgacaa
     1021 tcacaatgga acaattatca gtgatgcggt ccacattcaa gccggcagcc tgaataatca
     1081 aaatggcaac atcacaacac gccaacagtt agagattgaa accgatcaac tggataacgc
     1141 tcatggcaag ttattatcag cagaaatagc ggatttagcc gtttcaggca gcctgaacaa
     1201 tcaaaatggc gaaatagcga ccaatcaaca actgattatt cacgatggtc agcaatctac
     1261 cgctgtcatt gataatacga atggcacgat acaatcaggc cgtgatgttg ctattcaggc
     1321 aaaatcgtta tccaacaacg gcacacttgc cgctgataat aaactggata ttgcgttaca
     1381 agatgatttt tatgtagaac gcaatatcgt ggcgggcaat gaattgtcgc tcagtacacg
     1441 aggcagcctg aaaaattcac atactttgca agcaggaaaa cgcattcgga ttaaagcaaa
     1501 taaccttgat aatgcagcac aaggcaacat tcaatccggc ggtgcaacag acattggcac
     1561 gcagcacaat ttaaccaata gaggcttgat tgacggacaa caaaccaaaa tccaagccgg
     1621 gcaaatgaat aatatcggta caggtcggat ttatggcgac aatatcgcta ttgcggctac
     1681 ccgcttagac aatcaagatg aaaacggtac aggtgccgcc attgcggcac gtgaaaacct
     1741 gaatttaggc atcggacaat taaacaaccg tgaaaacagt ctgatttaca gcggtaacga
     1801 tatggcggtt ggcggcgcat tagataccaa tggccaagcc acaggcaaag cccaaaggat
     1861 acacaatgcc ggcgcaacca ttgaagccgc cggcaaaatg cgtttaggcg tagaaaagct
     1921 gcacaatacc aatgagcatt tgaaaacgca gttggtagaa acagggcgcg agcatattgt
     1981 tgattacgaa gcatttggac gacacgaatt attgcgggaa ggcacacaac atgaattagg
     2041 ctggtctgtc tataacgatg aatcagacca cttacgcacc cctgatggag cggcgcatga
     2101 aaattggcat aaatacgatt atgaaaaagt cacccaaaaa acccaagtta cccaaactgc
     2161 gccagccaaa atcatttcag gtaatgattt aaccattgat ggtaaagaag tatttaatac
     2221 cgatagccaa atcatcgccg gcggcaattt gcttgtgcaa acagaaaaag acggtttgca
     2281 taacgagcaa acctttggcg aaaagaaagt attcagtgaa aatggcaaat tacacagcta
     2341 ttggcgtgag aaacataaag gacgagactc aacgggacat agcgaacaaa attacacttt
     2401 gccggaggaa atcacacgca acatttcact gggttcattt gcctatgaat cgcatcgcaa
     2461 agcattaagc catcatgcgc ccagccaagg cactgagttg ccgcaaagca acggtatttc
     2521 gctaccctat acgtccaatt cttttacccc attacccagc agcagcttat atattatcaa
     2581 tcctgtcaat aaaggctatc ttgttgaaac cgatccacgc tttgccaact accgtcaatg
     2641 gttgggtagt gactatatgc tggacagcct caaactagac ccaaacaatt tacataaacg
     2701 tttgggtgat ggttattacg agcaacgttt aatcaatgaa cagatcgcag agctgacagg
     2761 gcatcgtcgt ttagacggtt atcaaaacga cgaagaacaa tttaaagcct taatggataa
     2821 tggcgcgact gcggcacgct ccatgaatct cagcgttggc attgcattaa gtgccgagca
     2881 agtagcgcaa ctgaccagcg atattgtttg gttggtacaa aaagaagtta aacttcctga
     2941 tggcggcaca caaaccgtat tggtgccaca ggtttatgta cgcgttaaga atggcgacat
     3001 agacggtaaa ggtgcattgt tgtcaggcag caatacacaa atcaatgttt caggcagcct
     3061 gaaaaactca ggcacgattg cagggcgcaa tgcgcttatt atcaataccg atacgctaga
     3121 caatatcggt gggcgtattc atgcgcaaaa atcagcggtt acggccacac aagacatcaa
     3181 taatattggc ggcattcttt ctgtcgaaca gacattattg ctcaacgcag gcaacaacat
     3241 caacagccaa agcaccgccg ccagcagtca aaatacacaa ggcagcagca cctacctaga
     3301 ccgaatggca ggtatttata tcacaggcaa agaaaaaggt gttttagcag cgcaggcagg
     3361 aaaagacatc aacatcattg ccggtcaaat cagcaatcaa tcagagcaag ggcaaacccg
     3421 gctgcaagca gggcgcgaca ttaacctaga tacggtacaa accagcaaac atcaagcaac
     3481 ccattttgat gccgataacc atgttattcg cggttcaacg aacgaagtcg gcagcagcat
     3541 tcaaacaaaa ggcgatgtta ccctattgtc agggaataac ctcaatgcca aagctgccga
     3601 agtcagcagc gcaaacggta cactcgctgt gtctgccaaa aatgacatca acatcagtgc
     3661 aggcatcaac acgacccatg ttgatgatgc gtccaaacac acaggcagaa gcggtggtgg
     3721 caataaattg attattaccg acaaagccca gagccataac gaaactgctc aaagcagcac
     3781 ctttgaaggc aagcaagttg tattgcaggc aggaaacgat gccaatatcc ttggcagcaa
     3841 tgttatttcc gataatggca cccggattca agcaggcaat catgttcgca ttggtacaac
     3901 ccaaactcaa agccaaagcg aaacctatca tcaaacccag aaatcaggat tgatgagtgc
     3961 aggtatcggc ttcactattg gcagcaagac aaacacacaa gaaaaccaat cccaaagcaa
     4021 cgaacataca ggcagtaccg taggcagctt gaaaggcgat accaccattg ttgcaggcaa
     4081 acactacgaa caaatcggca gtaccgtttc cagcccagaa ggcaacaata tcatccatgc
     4141 ccaaagcata gacattcaag cggcacacaa caaattaaac agtaatacca cccaaaccta
     4201 tgaacaaaaa ggcttaacgg tggcattcag ttcgcccgtt accgatttgg cacaacaagc
     4261 gattgccgtc gcacaaagca gcaaacaagt cggacaaagc aaaaatggtc gtgtcaatgc
     4321 catggcagct gccaatgcag gctggcaagc ctatcaaaca ggtaagagtg cacaaaactt
     4381 agccaatggt acaaccaatg ccaaacaagt cagcatctcc ataacctacg gcgaacagca
     4441 aaaccgacaa accacccaag ttcaagcaag ccaagcccaa gcgagtcaaa ttcaagcagg
     4501 cggtaaaacc acattaatcg ccacaggcgc agcagaacaa tccaatatca acatcgcagg
     4561 ctcagatatt gccggcaaag caggcacaat cctgattgcc gataacgaca tcacactcca
     4621 atcagccgag caaagcaata ccgaacgcag ccaaaacaaa tcgacaggtt ggaacgcagg
     4681 tgctgccgta tcattcggac aaggaggctg gtcattaggc gttaccgcag gcggcaatgt
     4741 cggcaaaggc tacggcaatg gcgatagcgt aacccaccgc catagccata ttggcgacaa
     4801 aggcagccaa acccttatcc aaagcggtgg cga
//
LOCUS       DAXHRI010000148         4819 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXHRI010000148 DAXHRI010000000
VERSION     DAXHRI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.148"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            95..901
                     /locus_tag="ACFM75_001776"
     CDS             95..901
                     /locus_tag="ACFM75_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC6256281.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(959..2224)
                     /locus_tag="ACFM75_001777"
     CDS             complement(959..2224)
                     /locus_tag="ACFM75_001777"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC6256282.1"
                     /translation="MSDAVAKETFNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYAEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            2631..3341
                     /locus_tag="ACFM75_001778"
     CDS             2631..3341
                     /locus_tag="ACFM75_001778"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC6256283.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSCNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            3414..3875
                     /gene="recX"
                     /locus_tag="ACFM75_001779"
     CDS             3414..3875
                     /gene="recX"
                     /locus_tag="ACFM75_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC6256284.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPYAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            3949..4110
                     /locus_tag="ACFM75_001780"
     CDS             3949..4110
                     /locus_tag="ACFM75_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256285.1"
                     /translation="MSGQPEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            4263..4745
                     /locus_tag="ACFM75_001781"
     CDS             4263..4745
                     /locus_tag="ACFM75_001781"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6256286.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
BASE COUNT         1259 a         1420 c         1100 g         1040 t
ORIGIN      
        1 agacagtttt cagacagcaa atccgtcttc acacgatacc tattttgtta taacataaca
       61 aaatctttaa cccacacgag acaaaggctg caccatgaag aaaacattga cactgctcgc
      121 cgtttccgcc ctatttgcca catccgccca cgcccaccgc gtctgggtcg aaaccgccca
      181 cacgcacggc ggcgaatacc ttaaagccga cttgggctac ggcgaatttc ccgaactcga
      241 acccatcgcc aaagaccgcc tgcacatctt cagcaaaccg atgcagctgg ttaccgaaaa
      301 aggcaaggaa aacatgattc aacgcggcac atacaactac cagtaccgaa gcaaccgtcc
      361 cgttaaggac ggcagttacc tcgtcatcgc cgaatatcag cctactttct ggtcaaaaaa
      421 caaagcaggc tggaaacagg cgggcatcaa agaaatgcct gacgcaagct attgcgaaca
      481 aacccgaatg ttcggcaaaa acatcgtcaa cgtcggacac gaaagcgcgg acaccgccat
      541 catcaccaaa ccggtcggac aaaacttgga aatcgtcccg ctggacaatc ccgccaacat
      601 tcacgtaggc gaacgcttca aagtccgcgt tctgttccgt ggcgaaccgc tgcccaatgc
      661 caccgttacc gccacctttg acggcttcga caccagcgac cgcagcaaaa cgcacaaaac
      721 cgaagcacag gctttctccg acagcacaga cgacaaaggc gaagtggaca tcatcccctt
      781 gcgccaaggc ttctggaaag ccaatgtcga acacaaagcc gacttccccg atcaaagcgt
      841 gtgccaaaaa caggcgaact actcgacttt aaccttccaa atcggccatt cgcaccatta
      901 atcccgcccg cacaaaaatg ccgtctgaag gcttcagacg gcattttttg ttcaaacatc
      961 aataccaacc gcgcagtttc atcgcttttt caacacggcg gatactcatc atgtaagacg
     1021 cggttcgcaa atcgacatcg tattcttgcg ccaagttcca tatatcgcgg aacgcacggc
     1081 gcaggacgac ggtttctttt tcctgaactt cgtcaaactc ccaataatag ccttgcagat
     1141 tttgtaccca ttcaaaataa gataccacca cgccgccgca gttcgccaaa atatcgggca
     1201 cgaccaatac gccgttttga cgcaggataa cgtcggcttc gggcgtagtc gggccgttcg
     1261 cgccttcgac cacgattgtc gcgcggactt taccggcatt ttcagaagtc agttggtttt
     1321 ccagtgcgca aggggcaagt acgtccactt ccagagccaa aagttcggca ttgctgattt
     1381 ctttaccgta gccggcttca ttggtgatga aacctttggt ttggaattct ttgatgattg
     1441 cttccatatc caagccgttt tcgttgtaaa tggcaacgtc aacagtagaa accgcaacaa
     1501 ctttcgcgcc ggattgatgc gcgtaataac ctgtgtggta accgacatta ccgaagcctt
     1561 ggatggcgta agtggcaccc ttcacgtctt tgcccagttt ttccaaggct tggacggcgg
     1621 cgaggttcac gccgtaaccc gtggcttcgg tacgcgccaa agagccgccg aactcgaccg
     1681 gtttgccggt aaatacgccc ggtgcggaat gtttcaccac gttttcataa gcatccacca
     1741 tccacgacat aattttgccg ttggtgttca catcgggggc gggaatgtcg attttctcgc
     1801 cgatgagcgg agaaatggct tctgcataag cgcgggcgat gcgttccagt tccgcctcgg
     1861 aataatcgcg cggatccaag gtaacgccgc ctttgccgcc gccgtaagga atgccggcca
     1921 cgcagcattt gatggtcatc caaatcgaca gggctttgac ttcgtccaga ttcacaccgg
     1981 gatggaagcg cacgccgcct ttataggggc cgacggcgtt gttgtgttgc gaacggtagc
     2041 ccgtgaaggt tttgaccgtg ccgtcgtcga gtttgacggg gaaattgact tccaacacgc
     2101 gggtcggact cttcaggatt tcataaacgg ccggatcggt tttcagccgg tcgcaggcgg
     2161 ttttgacctg ttttcgcgcg atttcaaacg gattgaaggt ttcttttgca acggcatcag
     2221 acattttgct tccttttcac aaaaagattt tcaggggcgt gcatttcgct cattactata
     2281 accaatttcc cgacaaaatg taatagcgtg tagtcggaat cagcccgatt tgattaatct
     2341 atatatgatt ttatttttca agccgcacgg aatcagcccg gactccgcct gaaaaagcag
     2401 cgcgcgcatc cgaaaaataa atgtcaaatt aaataaagat ataggcatcc ttttattttt
     2461 taaaaattta attgaaacat cgccgggatt tcaaaccctt gcccgattcc gccccgaaat
     2521 ccgaaaacac cgcctgcaaa acctgttgcg attgttaaca atctatacat tagaagccct
     2581 gtgcaaacga tgttaaaata aaccctttca acccgacagg aaatcagatt atgaatgcag
     2641 ccatcgaaca cgtccaagcc gtcgccttcg atttggacgg cacactgtgc gattccgtcc
     2701 ccgaccttgc cgccgccgca gaagcgatgt tggaacaact cggtatgaaa ccgctgcccg
     2761 ccaaagtggt cgaaagctat gtgggcgacg gcatcggcaa actggttcac cgcgtcctca
     2821 ccaacgaccg cgaccgcgaa gccgattccg aactgtggga aaaaggtttc gtatcctata
     2881 tgaaatacta ccgcgaccat ttgagcgtct tcacccgccc ctatcccgaa accgaagccg
     2941 ggctggcatt gctcaaatct ttgggcatcc cgctggcagt cattaccaac aaaaacgaaa
     3001 tccttgccgt cgaactgttg aaacagctcg gacttgccga ctacttcagc ctgatactcg
     3061 gcggcgacag cctgcccgag aaaaaaccca gccccctgcc gctgcggcat gccgccgaag
     3121 ttttgggtat cgatgttgca aacatggtta tggtcggcga ctcgtgcaac gacatcatcg
     3181 ccgccaaagc cgccggctgc ctgagcgtcg gcgttacctt cggttacggc gatatgacgc
     3241 tgctctcgca agacgatgcg acccgccccg actggattat cggctcgctg cccgaaattt
     3301 acgaaaacct gcaaccgcag aaaaacaaag acgaagaata aaacaatctg ttcagacggc
     3361 atcggaatcc gtttcccatg ccgtctgaat ctttccgtgc cgaaaccgcc gccatgaaac
     3421 cgcaaaaatc cctacgcgcc cgcgcgatgg acatcctctc gcgccaagaa gtcagccgca
     3481 tcggtctgaa acgcaaactt gcaccgtacg ccgaaagcga agaggagttg gaaaacgtgt
     3541 taaacgaatt tgccgaacgc aactggcagt cggatttgcg ctatgccgaa gcctatatcc
     3601 gcagcaaaag ccgcaaacac ggttcattga ggctgaaaca ggctttggcg caacagggca
     3661 tagatgaaga aaccagccgc aacctgcttc ccgaccgctc aagcgaaaaa caggccgcca
     3721 tagccgtgtt gcgtaaaaaa ttcaaacacc ccgccgccga cctcaaagaa aaacaaaaac
     3781 aggcgcgttt cctcgcctat cgcggattcg atgccgatac cgttcagacg gcattgaaac
     3841 acgcttggga cgaaaattgg gaagacggct gctgaccttc cttcttgaat ctttttgcac
     3901 ggcaacataa ccttacgccc gtttccaact tttccgattg agaacaaaat gtccggacaa
     3961 cccgaaaaaa atcataaccc cctccttgag gacgaacgca agaaccccgt gtaccgcatg
     4021 gggcaggcag ttgccggatt catgctcgtc gtttgggcag gcgtattggc actcgtgttt
     4081 ttcctggtct tccgtttttg gctttcctaa acaaaatgcc gtctgaaggc ttcagacggc
     4141 atcggcggcc catttctgca gactatcccg ccatagcttt ttttagcttg aattccactt
     4201 ccccattccc taaaattttc ccacacccat ttcaaaacac cctttcttaa aacaggtaca
     4261 ctatgacaca acaacgccaa ctgccttccc acgaactcat tatgtccgaa ctgatgatgc
     4321 cggacaccgc caatttcagc ggcaacgtac acggcggcga actcctgctc ctgctcgacc
     4381 aagtcgccta ttcctgcgcc agccgttaca gcggcaatta ttgcgttacc ctgtcggttg
     4441 acaaagtcct gtttaaagaa cccatccaca tcggcgattt ggttaccttt tacgccgctg
     4501 tcaactacac gggacgcacc tcgatggaaa tcggcatccg tgtcgaagca caaaacatcc
     4561 gcaccggcga aatccgccat accaacagct gctacttcac gatggttgcg gtcaaagacg
     4621 gcaaacccgt ccctgtccct ccgctggaaa tcctgaccga ccgccaacgc tgccgctacg
     4681 aaaaagccaa aaaacgcaga gacatcagcc tgcaagcctc tgaagacatg tcctgcggct
     4741 gctgacggcg gactatgccg tctgaaagac aggcacatcg cgccatccgt ttccattgca
     4801 aacggatgaa atcaagcaa
//
LOCUS       DAXHRI010000149         4780 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXHRI010000149 DAXHRI010000000
VERSION     DAXHRI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.149"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            110..514
                     /locus_tag="ACFM75_001782"
     CDS             110..514
                     /locus_tag="ACFM75_001782"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC6256287.1"
                     /translation="MTAQPCPICTAQDEDVLLQTAKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEIVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            554..1741
                     /locus_tag="ACFM75_001783"
     CDS             554..1741
                     /locus_tag="ACFM75_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC6256288.1"
                     /translation="MTEPTSRRRFLKTCTATAGAGLLQACGTAPASNNAPSAPSHPVA
                     KARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRR
                     FQRFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGTRMREHG
                     TLFFGFSDVCAVQLALLAKGNMMSFAGPMAYSEFGKLSPSVFTMDAFIKGATQNRLTV
                     DVPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLN
                     TLYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHI
                     ADKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVAD
                     ISE"
     gene            complement(1804..2337)
                     /locus_tag="ACFM75_001784"
     CDS             complement(1804..2337)
                     /locus_tag="ACFM75_001784"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC6256289.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(2516..4771)
                     /locus_tag="ACFM75_001785"
     CDS             complement(2516..4771)
                     /locus_tag="ACFM75_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC6256290.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSVLGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPDETFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPELNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTISAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
BASE COUNT         1190 a         1329 c         1202 g         1059 t
ORIGIN      
        1 tgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
       61 atcagaaatt atcctaccgt tttttaaaca ctttcaggaa taaggaaaaa tgaccgccca
      121 accctgcccc atctgcacgg cgcaagatga agacgttttg ttgcaaaccg ccaagctgcg
      181 cgtcatcgcc gtccataacg acagcggttc gcctgcattc tgccgcgtca tttggcgtaa
      241 gcatattgcc gaaatgaccg acctttcggc agcggaacgc ggcgaattga tggaaatagt
      301 gtacaaagtc gaagccgcta tgcgccaagt gttccggccg gcaaaaatca acctcgccag
      361 cttgggcaat gtcgtgccgc acctgcactg gcatattatc gcccgctttg aaaacgatgc
      421 gactttccct gcgccgattt gggcaaaccc cgtccggaaa cacggtatga ccctgccgca
      481 agattggacg gaacagctta aaaagctgct ttaagcccgc caatgccgtc tgaaaccgta
      541 tgaaagggaa attatgaccg aaccgacctc ccgccgccgt tttctgaaaa cctgcaccgc
      601 caccgcaggc gcggggctgc ttcaggcttg cggtactgcg cctgcttcaa acaatgcccc
      661 atccgcgccc tctcaccctg ttgcgaaagc ccgaaccgtg cctctccaaa cgccacgccg
      721 tcaaagttcg gacggcaacc ttctgcgcgt tgtcgcttcg tcaggatttg ccgaagacac
      781 caaccgcgtc aacacagcct taacccgcct ttacaatgcc ggttttaccg taaccaacca
      841 acaggcgggc agccgccgtt tccaacggtt tgccggtacg gacgcgcaac gtgccgccga
      901 tttccaagag gtcgcctccg gccgcgtcgc cacacctaaa gtgctgatgg gcttgcgcgg
      961 cggctatggt gcggcgcgga ttctgccgca tatcgatttt gcttcgctcg gcacaaggat
     1021 gcgcgaacac ggcacgctgt ttttcggatt cagcgatgtt tgcgccgtcc aactggcatt
     1081 gttggcaaaa ggcaatatga tgagttttgc cggcccgatg gcttatagcg aattcggcaa
     1141 actctccccc agcgtgttta caatggatgc ctttatcaag ggggcaaccc aaaaccgcct
     1201 gaccgttgat gttccttata tccaacgcgc cgatgtcgaa accgaaggca tattgtgggg
     1261 cggcaactta agcgtcctcg cctcgctcgc cggcacgcct tatatgcccg acatcgacgg
     1321 cggcattttg ttcctcgaag atgtcggcga acagccctac cgcatcgaac gtatgctcaa
     1381 tacgctgtat ctttcgggta ttttgaagaa acagcgcgcc atcgtgttcg gcaatttccg
     1441 tatggaaaaa attcgagatg tctatgatcc gtcttatgat ttttctgccg ttgccaacca
     1501 tgtttcgcgc acggcgaaaa tccccgtgct gacgggcttc ccgttcggac acattgccga
     1561 caaaatcact ttccctctag gcgcgcacgc ccgaatccgt atgaacggaa acagcggtta
     1621 ttcggtcgcg tttgaaggct acccgacact cgatgcgtcc gccctgactt tggataccct
     1681 gctcccaccg ccggatttgc ccatcttccc cgaaagcggt gttgccgata tttcggaata
     1741 aacccgcaaa cggacaaatg ccgtctgaag ccttcagacg gcatttccca agacggcggc
     1801 agattacagc aatgcccgaa tatcggcttc gatttcttcg ggcgtaacac taggcgcaaa
     1861 acgctcgacc acttcgccgt cgcggttgac gaggaatttg gtaaagttcc atttgatgtc
     1921 gccttcgtcg cgcttctctc ccaaagctgc gagcttcaac acgaaatctt taaacagatg
     1981 attgccttta tcttgcggtt tgacggattt caggtaggca tacaagggcg cggtatttgc
     2041 tccattgact tcgattttgt cgaaaatctt aaacttcgtg ccaaacttca tcatacacac
     2101 ttgggcaatt tctccgctgc tttcgggagc ctgttcgcgg aactggttgc acggaaaatc
     2161 caaaatctcc aagccttctg cggtatattg tgcatacagc ttctgcaaag cctcgtattg
     2221 cggggtcaga ccgcaacgcg ttgccgtgtt gacaatcagc agaaccttgc cgcgatagcc
     2281 tgacaaatca accgcattgc cttccgcatc tttcatttga aaatcgtaaa tacccatttt
     2341 tatccttatc tgatgtaaac cgatgccatc tgaaacgtgc ttcagacggc atgaaagcag
     2401 caattgtata gccgattaaa ataaaaaatc cacatccttt tccattcccg tcccaatccg
     2461 caataaaaaa ctgcacccga aaacgggtgc agttgctcat ttcataccgc aaaacttatt
     2521 tgtcgcggcc gaatacgatt ttagtggctt ggatggcaac gcagattgca ccgccgataa
     2581 agaccaagtc agctgccgta cgtacccaac gcaaggtgtc gaggatttcc atttgcagga
     2641 actcttcgct gcgtgcatac cacaggccgt gcgtgatgga ggcgtatgcc tgaatcacgc
     2701 caaccggcaa caggctgatg gcaatcatac cgaccaagcc gccgttgagc agccagaagc
     2761 cccaagtcat cagtttgtcg tcaaactgcg cgttcggttt caaataacgg gcaaccagca
     2821 atacgaagcc caatgccaag aaaccgtaca caccgaacaa ggcggcgtgc gcatgaacag
     2881 cagaagtatt caaaccttgg atatagaaca gggaaatcgg cgggttaatc aggaaaccga
     2941 atacaccggc accgatcata ttccaaaagg cgactgccac gaagcacatc agcggccaac
     3001 gcaggcgttt cgcccagtcg gacaggtgtt ggtaagacca atgctcgtat gcttcacggc
     3061 ccagcaacac cagcggcacg acttccaaag cggagaagca ggcaccgatt gccatagagg
     3121 cggaggtaga gccggagaag tacaggtggt gcagcgtacc cggaacgccg cccaacataa
     3181 agatggcggc agcggccaga gtagaagctg tggcggtact gcggcggaca aagcccatat
     3241 tgtagaagac aaaggcaaaa gcggcagtgg caaatacttc aaagaagcct tccacccaca
     3301 ggtgaaccac ccaccaacgc cagtattcca ttacggcaat cggggatttt tcgccataga
     3361 acaggcctgg tgcgtagaat acgcccacac cgaccataga agcgacaaag attgccagca
     3421 ggtttttgtc cacgcctttt tctttaaagg cggagatggt gcaacgcagc atcaggaaca
     3481 gccacaacag cagaccgacc atcagcagga gttgccagaa acggcccaaa tcgaggtatt
     3541 cgtaaccttg gtgtccgaac cagaaattca attcgggggg aaggatgtgc gtcaacgcga
     3601 agaagttgcc cgcgtaagaa ccgccgacca cgatgaagag ggcgatatag aggaagttca
     3661 cgcctgcacg ttggaacttg ggatctttgc cgccgttgac aatcggcgcg aggaacaaac
     3721 ctgccgtcaa aaagccggtt gcaatccaaa agatggcgga ttggatgtgc caagtacggg
     3781 tcagggcata gggaaaccag tcggacattt caaagcccaa cgcctcatcg atgccgtaga
     3841 aaccctgacc ttcaacggtg tagtgcgcgg tcaggccgcc cagcaatact tgtaccacaa
     3901 acagggcaac cgtcaggaag acgtatttgc ccaatgcttt ttgcgaaggg gtcagttgga
     3961 ttttggaaat cgggtcttca gacggcactt ccacttcctc gtgtttggtc aggaaggaat
     4021 aaccccacat caacaaaccg atgcccatca agagcagtac aacactggta aacgaccaca
     4081 tatagttttc agtggtcggt acgttgttga tcaaaggctc gtgcggccag ttgttggtat
     4141 aagtgaacgt ctcgtcagga cggttggtcg aagcagacca agaagtccag aagaagaagt
     4201 cgaacagttt ttcacgcgct tcttggcttg gcaatgtgtt gtttttcatt gcaaagtgtt
     4261 cgcgggtggt ttgcagcgcg ggatcatcgc cgtaaacacc gtgataataa ggcaggatgc
     4321 tttcgatggc tttcacgcgc gtatcgctga tgacgacgct gccgtcttct ttaatacggc
     4381 tttggttgcg gtattcgtca gccagtcggg ttttcagaac ggcttgttct tcaggggaaa
     4441 cttcatcgaa ttttttgccg taagtctgtt gcgcggtcaa atccaaccag gcggacaact
     4501 cacgatgcag ccagtcggca gtccagtccg gagcttggta tgcaccgtga cccaatacag
     4561 agccgacttc cataccgccg gtagtctgcc atgcagactg acctgccaaa atatcgtctt
     4621 tcgtcatcaa gactttgccg gatgcggaaa cgacctgttc ggggtaaggc ggggctttct
     4681 tgtaaacctc gctgcccata tagccaagaa tggtaaagca taccgccaga acggcaaaca
     4741 gcaagtacca cagcttcttg tactgtccca ttttgagagc
//
LOCUS       DAXHRI010000150         4726 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXHRI010000150 DAXHRI010000000
VERSION     DAXHRI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.150"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            165..944
                     /gene="pgeF"
                     /locus_tag="ACFM75_001786"
     CDS             165..944
                     /gene="pgeF"
                     /locus_tag="ACFM75_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC6256291.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            996..1475
                     /gene="lptE"
                     /locus_tag="ACFM75_001787"
     CDS             996..1475
                     /gene="lptE"
                     /locus_tag="ACFM75_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC6256292.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1477..2475
                     /gene="holA"
                     /locus_tag="ACFM75_001788"
     CDS             1477..2475
                     /gene="holA"
                     /locus_tag="ACFM75_001788"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC6256293.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            2596..3015
                     /locus_tag="ACFM75_001789"
     CDS             2596..3015
                     /locus_tag="ACFM75_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256294.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3090..3605
                     /locus_tag="ACFM75_001790"
     CDS             3090..3605
                     /locus_tag="ACFM75_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6256295.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALIAGIAAIFLNPV
                     SIAVSAWVTSILIMESWWLYFRANWHGQGQTLAMRTWRIGLCDVNGMQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3689..4573)
                     /locus_tag="ACFM75_001791"
     CDS             complement(3689..4573)
                     /locus_tag="ACFM75_001791"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC6256296.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIVRGKVECKIQIQDAENGSQSLELNRDLVGQLAEINQTLR
                     KHHDLAKLGVADILRFPGVLASQRESTEELAKSITELTEKALKDFTAARKREGKKLGE
                     HLLQRLEAMEEIIDALSELFPTLLETHKEKIRARLVEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTRAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1063 a         1248 c         1319 g         1096 t
ORIGIN      
        1 taagggcatc attcccgcac ctgcaagaat ccgaatccga acgtttgaaa acaatcccga
       61 atctccgaat tcccgcctgt gcgggaatga cgaaaaaaca agcatccatt tgccccgaag
      121 gcagttaatc aaccttttcc gccacacacc tattccaata tccaatgaaa accatcacag
      181 aaaccctaaa tcttgccccg aaaggcaaaa acttcctgac cgccgattgg cccgcgcccg
      241 ccaatgtgaa aaccctgatt accacgcgca acggcggcgt gagccaaggt gcgtatcaga
      301 gtttgaacct cggtacgcac gtcggcgaca atcccgaagc cgtgcgccgc aaccgcgaaa
      361 tcgtgcaaca gcaggtcgga ttgcccgttg cctacctcaa tcaaatccac agcaccattg
      421 tcgtcaatgc tgccgaagca ttggacggca cgcccgatgc ggacgcttcc gtggacgaca
      481 cgggcaaggc cgcctgtgcc gtgatgaccg cagactgtct gcccgttcta ttttgcgaca
      541 gggcgggtac ggcggttgcc gccgcacacg cgggctggcg cggtttggcg ggcggcgtat
      601 tgcaaaacac cattgctgcg atgaaggttc cgcctgtcga aatgatggcg tatctcggcc
      661 ccgccatcag tgcggatgcg tttgaagtcg gacaggatgt gtttgatgcg ttctgcacgc
      721 ccatgcccga agccgccgcc gcatttgaag gcataggcgg cggcaaattc cttgccgacc
      781 tttacgcgct cgcccgcctg attctgaagc gtgaaggcgt gggcggcgta tatggcggca
      841 cgcattgtac ggttttggaa cgggacactt tcttttccta ccgccgcgac ggagcgacag
      901 ggcgtatggc gagcctgatt tggctggacg gcaatgccgt ctgaacacgc cgctgatata
      961 atctaccgac tttgtgcttt tgagaaaggc aagccatgaa caaactgttt cttactgccg
     1021 cagtgctgat gctgggcgcg tgcggtttcc acctgaaagg tgcagacggc atttctccgc
     1081 cgctgaccta ccggagctgg cacatcgaag gcggacaggc attgcagttt cctttggaaa
     1141 ccgcgctgta tcaggcttcg ggcagggtgg acgatgctgc cggcgcgcag atgaccctgc
     1201 gtatagacag cgtttcccaa aacaaggaaa cctacaccgt tacccgtgcg gcagtcatca
     1261 acgaatatct tttgatattg acggttgaag cgcaggtatt gaaacgcggc gagccggtcg
     1321 gcaaaccgat gaccgtgtcc gtccgccgcg tccttgctta tgccgacaac gagatcttgg
     1381 gcaaacagga agaggaagcg gcattgtggg cggaaatgcg gcaggatgcc gccgaacaga
     1441 ttgtccgccg cctgaccttt ctgaaggcgg aatgacgtgg cggcacatat cggacgcatt
     1501 gatacggacg cgcctttgaa acccctgtac gtcatccacg gcgaggaaga actgttgcgt
     1561 atcgaggcat tggacgcatt gagggcggcg gcgaagaaac aaggttacct caatcgggaa
     1621 gtttatacgg cagacaatgc cttcgattgg aacgaactgt tgcaaaccgc aggcagtgcg
     1681 ggtctgtttg ccgatttgaa gctgttggaa ctgcatatcc ccaacggaaa acccggcaaa
     1741 accggcggcg aggcgttgca ggattttgcc gcccgcctgc cggaagatac ggtaacgctg
     1801 gttttgctgc ccaaactgga gaaaacccag ctccagtcca aatggtttgc cgcattggcg
     1861 gcaaaggggg aagtgtggga agccaaaccg gtcggcgcgg cggctttgcc ccaatggata
     1921 cgcggacggc tggacaaaat cggtttgggt atcggggcag acgcattggc actgtttgcc
     1981 gagcgcgtgg aaggcaatct gttggcggca cgtcaggaaa tcgacaagct cgcgctgctg
     2041 tatccgaaag ggcataccgt caatatcgat gaggcgcaaa ccgccgtcgc caacgtcgcc
     2101 cgctttgacg cgttccaact ggcaggcgcg tggatgaagg gcgatgtccc gcgcgtatgc
     2161 agattgttgg acggattgca ggaggaaggc gaagaaccgg tgctgcttct gtgggcggtt
     2221 gccgaagacg tgcggacgct gatccggctt gccgccgccc tgaagcaggg gcagagcatc
     2281 caatccgtcc gcaacagcct caggctttgg ggcgacaagc agacgctcgc accgcttgca
     2341 gtcaagcgga tttccgtcgt ccgcctgctt gacgcgctca aaacctgtgc ccaaatcgac
     2401 cgaatcatca aaggcgcgga agagggcgac gcatggacgg tattcaaacg gcttgtcgtg
     2461 tcgctggcgg aataaagcgg taatccccaa aatccgaaaa tactgtaaaa taccgttaat
     2521 cctgaaaagt attcaccaat ccgtccgaaa acatttcaga cggcacgacc acctcaataa
     2581 aggaacatta accctatgga caataagacc aaactgcgct tgggcggcct gattttactg
     2641 accaccgccg ttttaagcct cattatcgta ttgattgtcg attcctggcc gcttgccatc
     2701 ctgcttgcag ccgtcatcgt cgctgccgct gcgggcggtt ttgtttggac atcccgccga
     2761 cagcaacgcc agtttatcga acgcctgaaa aaattcgaca tcgatcccga aaaaggcaga
     2821 atcaacgagg caaacctgcg ccgtatgtac cacagcggcg gacaacacca gaaagatgcg
     2881 attaccctga tctgcctgtc gcaaaaatgt tcggtggacg aggcgcacgc tatgttcaaa
     2941 aaacgcccga cacgtcagga aatcaatcaa atggcggcaa aacagtcgcg cggtcagaaa
     3001 cgtccgcacc gttaaccgtc cgaggcatat ctgtatgaat accgtctgaa gcctgttggc
     3061 gtttcagacg gcatattccg attgaaaaga tgatgatact gaaaaccgcc ccgctcaaac
     3121 gccgctttgc cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag
     3181 cattgattgc cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat
     3241 gggtaacaag tatcctgata atggaatcat ggtggcttta tttccgtgcc aactggcacg
     3301 ggcagggaca gaccttggcg atgaggacat ggaggatagg cttgtgcgat gttaacggta
     3361 tgcagccgtc tctacacttg ctccgcctgc gctttatttg ggcgtgcata ttcatcgtct
     3421 tcattcctat gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg
     3481 cggccggtgc ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg
     3541 atcggcagtt tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc
     3601 cttaagcctt tataccgcaa aggtttcaaa ctgaaaaaat gccgtttgaa agggctttca
     3661 gacggcattt ggttatcggg gaaaccgatt attcgatatt ttgcacttgt tcccgcatct
     3721 gctcgattaa gactttcagt tcgacagagg ctcgggtgca ttcggcggca atggatttgc
     3781 tgcccaaggt gttggcctca cggtttaatt cctgcatcag gaaatccaag cgttttccgc
     3841 tgctgccttt gtgttcggta acgatgcggc gcacttcggc aatgtgggtg cgtaggcggc
     3901 tgaactcttc gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt
     3961 cgttgtcgat gctgccgacc gcttcgacga ggcgggcgcg gattttttct ttatgtgttt
     4021 ccaacagggt aggaaagagt tcgcttaatg catctatgat ttcttccata gcctcaaggc
     4081 gttgcagcag gtgttcgcct aatttcttac cttcccgctt gcgtgcggca gtgaagtctt
     4141 ttaacgcttt ttcggtcagt tcggtaatgc tttttgccaa ttcttccgta ctttcccttt
     4201 ggcttgccaa tacgccggga aaacgtaaaa tatcggcaac ccctaatttt gccaggtcat
     4261 gatgtttacg gagggtttgg ttgatttctg ccagttgtcc gaccaaatcg cgattcagtt
     4321 ccaaggactg actgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc
     4381 gcacgatatg ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcatccg
     4441 gcatcctgat ttgaatgtcc aaaaagcggt ggttgacggc gcggatgtcg agattgatgc
     4501 gtttgctgcc gcactctgcc gccgcgttgg caaatccggt catgctgtgg atgtggatat
     4561 ttccgctgct catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt
     4621 atggcggcaa gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc
     4681 acgcgctttt gttgctgcaa ggaagggaaa tgtatagtgg attaac
//
LOCUS       DAXHRI010000151         4531 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXHRI010000151 DAXHRI010000000
VERSION     DAXHRI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.151"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            168..1400
                     /locus_tag="ACFM75_001792"
     CDS             168..1400
                     /locus_tag="ACFM75_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC6256297.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQHFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            1439..2566
                     /gene="pheA"
                     /locus_tag="ACFM75_001793"
     CDS             1439..2566
                     /gene="pheA"
                     /locus_tag="ACFM75_001793"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC6256298.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGITKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASSSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRQDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            2600..3391
                     /gene="recO"
                     /locus_tag="ACFM75_001794"
     CDS             2600..3391
                     /gene="recO"
                     /locus_tag="ACFM75_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC6256299.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVSFVPVSASWYGSQELKTLHRAEWVGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            3415..4143
                     /gene="pdxJ"
                     /locus_tag="ACFM75_001795"
     CDS             3415..4143
                     /gene="pdxJ"
                     /locus_tag="ACFM75_001795"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC6256300.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            4189..4296
                     /locus_tag="ACFM75_001796"
                     /pseudo
     CDS             4189..4296
                     /locus_tag="ACFM75_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4295..4516
                     /locus_tag="ACFM75_001797"
                     /pseudo
     CDS             4295..4516
                     /locus_tag="ACFM75_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine catabolism dioxygenase TauD"
BASE COUNT          977 a         1256 c         1297 g         1001 t
ORIGIN      
        1 aggcaacgct gtactggttt ttgttaatcc actatatcgt tccggttcgt ccggttttgc
       61 cggggctttt gttgccgcct gtttgtgccg gtgtgttaaa attttccgtt tccgcgtatt
      121 gtgttttccg ccgccgggcg gtttgtttgc gaatcggacg agaatttatg ccttctgccc
      181 attatcctga aatgagcgaa aaactgatgg cggttttgat ggcgatgctg gttacgctga
      241 tgccgttttc catcgatgcc tacctgcccg cgattcccga aatggcgcag tcgctgaacg
      301 cggatgtcca ccgcatcgaa cagagcctga gtttgtttat gttcggcacg gcgttcggac
      361 aggtggtcgg cggttcggtg tccgacatca aagggcgcaa acccgtcgcg ctgaccggtc
      421 tgattgtata ttgccttgcc gttgccgcca tcgtatttgc ttcgagtgcc gaacagctcc
      481 tcaacctgcg cgtcgtgcag gcattcggtg cgggcatgac tgtggtcatc gtcggcgcaa
      541 tggtgcgcga ttattattcc ggacgcaaag ccgcccagat gtttgccctt atcggcatca
      601 ttttgatggt tgtgccgctg gtcgcaccca tggtcggcgc attgttgcag ggcttgggtg
      661 gctggcaggc gatttttgtt tttctggcgg cgtattcgct ggtgctgctc ggtttggtgc
      721 agcatttcct gcccaagcct gccgtcggcg gcaaaatcgg acgggacgtg ttcgggctgg
      781 tggcggggcg gttcaagcgc gtattgaaaa cccgtgccgc catggggtat ctgtttttcc
      841 aagcattcag tttcggctcg atgtttgctt ttttgaccga atcgtccttc gtgtaccagc
      901 aactttaccg cgttacgccg catcaatacg cttgggcgtt tgcgctcaac atcatcacga
      961 tgatgttttt caaccgcgtt actgcgtggc ggctcaaaac cggcgcgcat ccgcaaagca
     1021 tcctgctgtg ggggattgtc gtccagtttg ccgccaacct gtcccaactc gccgccgtgc
     1081 tgtttttcgg gttgcccccg ttttggctgc tggtcgcgtg cgtgatgttt tccgtcggta
     1141 cgcagggctt ggtcggtgca aacacgcagg catgctttat gtcttatttc aaagaagagg
     1201 gcggcagcgc gaacgccgtg ttgggcgtgt tccagtcctt aatcggcgcg ggggtgggta
     1261 tggcggcgac cttcttgcac gacggttcgg caaccgtgat ggcggcaacg atgaccgcgt
     1321 ccacctcttg cggcattgcg cttctgtggc tgtgttcgca ccgggcgtgg aaggaaaacg
     1381 aacagagcga atacctttaa cggaaaatgc cgtctgaaac cgtttcagac ggcatttgat
     1441 gttagaatgc acgataaatt actgttcagg cgaaattatg tcccaaacta tcgacgaact
     1501 cctccttccc caccgcaacg ccatcgacac catcgatgcc gaaatcctgc gcctgctcaa
     1561 cgaacgtgcg caacacgccc acgccatcgg cgagctgaaa ggcacgggcg cagtgtaccg
     1621 ccccgaacgc gaagtcgccg tgttgcgccg cattcaggat ttgaacaaag gcccgctgcc
     1681 cgacgaatcg gtagcacgcc tgtttcggga agtgatgagc gagtgcctcg ccgtcgaacg
     1741 cccgctgacc atcgcctatc tggggccgca gggcacgttt acccaacagg cggcgattaa
     1801 gcatttcgga cacgccgcgc acaccatggc gtgtccgacc atagacaact gcttcaagca
     1861 ggttgaaacc cgtcaggcag actatctggt cgcccctgtg gaaaactcga ccgaaggctc
     1921 ggtcgggcgc acgttggatt tgcttgccgt taccgcgttg caggcgtgcg gcgaagtcgt
     1981 tttgcgcatc caccacaacc ttttgcgtaa aaacaacggc agcaccgaag gcatcaccaa
     2041 agtcttttcc cacgcgcagg cgttggcgca atgcaacgac tggttgggca gacaccttcc
     2101 caacgccgaa cggattgccg tgtccagcaa tgccgaagcc gcaaggctgg ttgccgaatc
     2161 ggacgacggt acggttgccg ccatcgccgg acgcacggcg gcggaaatct acggactcga
     2221 tatggttgcc gagtgcatcg aagacgagcc gaacaacacc acgcgcttct tggtgatggg
     2281 acatcacgaa accggtgcaa gcagcagcga caagacttcg ctggccgttt ccgcgcccaa
     2341 ccgcgccggt gcggttgcct cgctgctgca accgctgacc gagtcgggta tttccatgac
     2401 caagtttgag agccgtccga gcaaatccgt tttgtgggaa tacctgttct tcatcgacat
     2461 cgaaggacac cgccaggacg cgcagattca gacggcattg gaacgcttgg gcgaacgcgc
     2521 ttcgttcgtc aaagtcatcg gttcgtaccc gaccgccgtt ttgtagcggc agcagcgttc
     2581 agacggcatt tcaggcatga tgtccgaaca ccgagtcaac cacgaacccg tcttcctgtt
     2641 ggcttcctcg ccatggcgcg aaagcagcct gcgggtcgaa gcgttcagcc gccgttacgg
     2701 gcgtgtggca cttttggcaa gaagcgcgcg caagcggcag agcgaattgc gcggcgtatt
     2761 ggtttctttc gtgcccgtca gtgcgtcgtg gtacggcagt caggaactca aaaccctgca
     2821 ccgtgccgaa tgggtcggcg gatggcggca gccgcagggc agggcgttgt tcagcggtct
     2881 gtatgtgaac gaactggtgt tgaaactgac cgcgcgcgaa gacccgatgc ccgaactgta
     2941 cgacgcgttg gcaaaagtga tggaggcggt gtgccgtgaa gccaaccata ttgccgattt
     3001 gcgccgcttc gagtggaagc tgctcaatgc cttgggcgtt gcccccgatt tgcacgccga
     3061 cggcacgggc ggcgatattt tggcggacaa aacctaccgg ctgatgcccg aagaggcggt
     3121 catgcccgta tgcgaggacg cagacgcgct ttcgcacgaa gccggcgcaa ttgtcgaagg
     3181 gcaaagcctg atcgatttgc gcgaaggcag tttccgcacc gccgaaagcc tgcaacaggc
     3241 attgaaaatc acacggcttt ttatccgcca cctgttgccc gaggggctga aatcgcggca
     3301 ggtgttggaa cagatacggc agtttgaccg caaagaaacc gcccgggaaa ccgtcccgac
     3361 ttcggacggc acggcttcaa atgccgtctg aaggcagaaa taaaaggaaa gattatgctt
     3421 ttaggtgtca acatcgacca catcgctacc gtccgcaatg cgcgcggcac gacttatccc
     3481 agccccgtgg aggcggcact ggttgccgaa acgcacggtg cggatttgat taccatgcac
     3541 ctgcgcgaag accgccgcca catcaaagac gcggatgtgt ttgccgtcaa aaacgccatc
     3601 cgcacgcgcc tgaaccttga aatggcgttg acggaagaaa tgctcgaaaa cgcgcttaaa
     3661 gtgatgccgg aagacgtgtg catcgtgcct gaaaaacgtc aggaaatcac gaccgaaggc
     3721 ggcttggacg tattggcgca acaggaaaaa atcgccgggt tcaccaaaat cctgaccgac
     3781 gcaggcatcc gagtctctct ctttatcgat gccgacgaca ggcaaatcca agccgcctat
     3841 gatgtcggcg cgcccgttgt cgagctgcac acaggcgcgt atgccgacgc gcgcagccac
     3901 gccgaacaac tcaagcaatt cgagcgtctg caaaacggcg cgcattttgc cggcgatttg
     3961 ggcttggtcg tcaacgccgg acacggactg accatacaca acgttacccc catcgcccaa
     4021 atcctcgcca tccgcgaact gaacatcggg cattcgctga ttgcccaagc cctcttcctc
     4081 ggactgcccg aagccgtgcg ccaaatgaag gaggcgatgt tcagggcaag gctgctgccg
     4141 taagggcagg caaacccttt cagacagcat ttcacgacag ggatatgtta tagtggatta
     4201 aatttaaatc aggacaaggc ggcgaagccg cagacagtac aaatagtacg gcaaggcaag
     4261 ccaacgccgt actggtttaa atttaattca ctatatgaat caaaagtata ttttatctgc
     4321 aaacaataat agtttgatag aagaaattca caatacagta cagcgtattg ggtattgtat
     4381 tgttcgaggt cttaatctaa accatcttga tggcagccgg agaaacaaga aattatttga
     4441 ctttctatct caattaggaa tgctgacaaa ccacaaaggc gatggtttta aatctatatt
     4501 ttgggatatt aaatattgag gcgatgatta t
//
LOCUS       DAXHRI010000152         4527 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXHRI010000152 DAXHRI010000000
VERSION     DAXHRI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.152"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFM75_001798"
     CDS             complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFM75_001798"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6256301.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIVLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1846..2133)
                     /locus_tag="ACFM75_001799"
     CDS             complement(1846..2133)
                     /locus_tag="ACFM75_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFC6256302.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            2190..2468
                     /locus_tag="ACFM75_001800"
     CDS             2190..2468
                     /locus_tag="ACFM75_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256303.1"
                     /translation="MIVIIFINRIINQIFMDDCTGLYVPFPTFFANTRKGGLSDGIKL
                     PPLPCLTLLPNGIIRIVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(2601..3482)
                     /gene="nadC"
                     /locus_tag="ACFM75_001801"
     CDS             complement(2601..3482)
                     /gene="nadC"
                     /locus_tag="ACFM75_001801"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC6256304.1"
                     /translation="MPSENTLFPLPDPLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGISTATARAVAEVAKYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3557..4495)
                     /locus_tag="ACFM75_001802"
     CDS             complement(3557..4495)
                     /locus_tag="ACFM75_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256305.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1036 a         1138 c         1218 g         1135 t
ORIGIN      
        1 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
       61 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      121 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      181 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      241 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      301 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      361 aaaaattcat caatatcggt actggatatt ttgtcatcac taataaacat cgctagagat
      421 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      481 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      541 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      601 tatagttatt gcctttggga gaatcaattc tgacagcaca taacataggt tcatctttac
      661 agtcgttata ttgggtttca tcaaactcaa ctgcatagca caataaaggc aataggaaaa
      721 aaaggagatt aatttattac tatacataag gcagcctgac tattcattct aaaataaatc
      781 cggtttttaa tatttgattc tgctccggat tatatacata ataaatttta ctgctccaaa
      841 atctgatatt aaccctatgg atttcataaa tttttcagaa aaataggctg tgcttgaaca
      901 atccgccgac tccggctgtt tgtcagggaa aaggcaatta ttttcggtta gtcagcagcc
      961 tatcctattg aatcacttaa agatatggat gtttgtcaac gccatatcaa aacacggtct
     1021 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1081 tctccaaatc aacgcggaca tccttcaacg ccgccgccat cgcaggcgac accaaaatat
     1141 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccaggtat
     1201 agaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1261 ttttgaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1321 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc cagtagcacc agtttgccgt
     1381 agcgggtcat ttcatcgcgg acaaattcgg ttaacgcggc aaaccattgt tctttatatt
     1441 tgaatttcgg gctgtcgagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1501 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcacga
     1561 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1621 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1681 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1741 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1801 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac accataccgc
     1861 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     1921 gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc agaccgcccg ccctcaggcg
     1981 gttcaaatgg ccggaaacgg cggtggccgg cagggataag gattttgcca gttcggcgat
     2041 attgcgttcg ctgtccaaca gttggatcag tatcgccata cgttcgggat gcgccataag
     2101 tttgagtatg gtgtggagcg gtattgtgtt catcggtatt cctaaaacct ttgccgcccg
     2161 ccaagcggat tgacgggcga cagatatgga tgattgtgat tatctttata aatcgtatta
     2221 taaatcaaat atttatggat gattgtacag gattgtatgt tccttttccc actttttttg
     2281 caaacacgcg taaaggcggg ctttcagacg gcataaaact gccgccccta ccctgcctca
     2341 ccctgttgcc gaacggaatc atccgcattg ttcaaaagat gccttacaac cgccgcacgt
     2401 cttgccctgc cgatgcctcc gtgccttcgg gcaaacaaaa tacctgcctg aaacgctgtt
     2461 ttttctaaag catcacgacc ttcacgaaaa atgccgtctg aagaatattt cagacggcat
     2521 ttcctccttg aggcaataac cggattacgg ttaggcggtt ccttgtctga catatcagac
     2581 aactgccgac accctaaaac tcatgccaag aaatccaaac ctatgtccaa cgagcggctg
     2641 ctgtgggtca gatagccgag ggcgatgccg tccacgccgg tttgcgccac gcgcttcagg
     2701 cggtcgaagc cgatgccgcc cgatgcttcg caatagacgg tgtgggggtg ggcggtttgc
     2761 gtgtggcagc ggtttgccgc ttctttcagg gtttcgtcgt ccatgttgtc cagcaaaatc
     2821 cgttccgcgc ccgctgcgat ggcttcgtcc agttgtgcca acgtgtccac ttcgatttcc
     2881 acgcaggtca atgctccgac cgcctgtttt gcctgccgaa cggcttgggc gatgctgccg
     2941 caataggcga ggtggttgtc tttgatgagt acggcatcgt ccaaacccat gcggtggttc
     3001 acgccgccgc ccgccctgac ggcgtatttt tgcaggacgc gcagcagggg gatggttttg
     3061 cggctgcaca cgatgtctgt accgtatttg gcgacttcgg caacggcacg cgcggtggcg
     3121 gtggagatgc cgctcaagtg cgtgaggtag ttgagcgcgg tgcgttcggc ggcgagcagc
     3181 gcgcgggcgt tgccttcgac ggcggcaagc gtctgacctg cgcggacgac ttgcccgtct
     3241 cggatttcgg cttggaagcg gacggacgga tccatcgtct gaaaggcgag gcgcgccaag
     3301 tccataccgg cgataacacc gtcttcgcgg ctgacgagga agagtttggc ggttttgtcg
     3361 ggggcgatga cggcggcgga cgtaatatcg ccgcgcctgc ccaaatcttc gctcaaggct
     3421 tgttccacca tggggcgcaa cagggggtcg ggcaggggaa agagggtgtt ttcagacggc
     3481 atggggtttc ctttgttgaa aaagatgggt gttgcaccga tgggcaaaac ttcgtttgat
     3541 gccgtctgaa aacgggctaa cggctgccca gcggcagtcc gatgtccgaa atcagcctgt
     3601 cgggcaggac gtcgtcgcgg aagcggcaaa gctgcgcggg acggcctttg ctgcccgata
     3661 cgccggtatc cgacggctcg atgaggtttt gctgctgaat ctggcggcgg aagttttgct
     3721 tgtgcagcaa tctgccgctg atggcttcga cgctgttttg cagttgcagc agcgtgaatt
     3781 cgggcggcat cagttcaaaa atcacggggc ggtatttgat tttggcgcgc aggcgagaca
     3841 gggcggtcgc cagtacgcgg cggtggtcgt ggcgcatggg ctgccccgtg agcgcgaagt
     3901 cgaagtttgc ctgcggctcg gcggcttcca ctatcagacc gctttcatac agcatttcat
     3961 agcgttgtaa aacgtattct tccgaccagt tttccggttc gaccccccag cacaaatgaa
     4021 tgcgcttgag ccgcatttga cgcacttcct ccgtgtccgc cgaatttgcc caaatgcgca
     4081 ggcggctgac gacggcgtcg cgctgtccgc cgtcggtgcg caagtcttcc cacgggaaat
     4141 agccgtagca gtcctgccat ttcgcatccg ggtgcaggat gctgtcggct gcctcgcgca
     4201 ccagccccaa atagctgacg tacaagacgg gcatgccgtg ttcgttgcgg cggtgggtat
     4261 cgacaaaggt gtaaagctgt tccacatagc ccataggctg cgaagtctgc ttggcaaccc
     4321 acagcttcac gcccgcctgc agggaattgc gcagagggga gagcgggccg ttgggcagga
     4381 gtgtgccttg ggcgacggtt aatacccgca ggccgccgtc ggtaacggca atcaatacgg
     4441 gaaccagctc gacgatgctt tgcggcgggg cttcggcttc ggggtaggca tccatgctcg
     4501 gctttctgcg ggtgggaaag gctttat
//
LOCUS       DAXHRI010000153         4520 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXHRI010000153 DAXHRI010000000
VERSION     DAXHRI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.153"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(132..1070)
                     /locus_tag="ACFM75_001803"
     CDS             complement(132..1070)
                     /locus_tag="ACFM75_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6256306.1"
                     /translation="MLELNGLCKRFGDKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1079..1954)
                     /locus_tag="ACFM75_001804"
     CDS             complement(1079..1954)
                     /locus_tag="ACFM75_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6256307.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWVERVKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLALGWHCAGLLDGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSETVCRESSEEAGLDKTLLPLIRPVSQLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLAAMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2136..2212)
                     /locus_tag="ACFM75_001805"
     tRNA            complement(2136..2212)
                     /locus_tag="ACFM75_001805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2176..2178),aa:Met,seq:cat)
     regulatory      2362..2461
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2552..4453
                     /gene="thiC"
                     /locus_tag="ACFM75_001806"
     CDS             2552..4453
                     /gene="thiC"
                     /locus_tag="ACFM75_001806"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC6256308.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1083 a         1302 c         1200 g          935 t
ORIGIN      
        1 tttgcgggaa tgacggcagg gtggtttctg ttgtttcgga taaattttgc agccctgata
       61 aaaaatatgg ctgctttggt aaaaaatgcc gtctgaaagg ttttcagacg gcattttgtt
      121 tttaagaagc atcagcggaa gcggacgatt tccctgtctt cgatatggat gcgtaccgta
      181 tcgtttcccg atatttcacc ggcgtgcggc atatcgaggt tcagccacag gatgccgtgt
      241 tccggatgga ggacggacag gctgaacgat tcgggcaaac aggtacggga taatacgcgg
      301 cactccatgc cgtcttggtc gaaacgcacc gcatgttgcg gaatatggcg gtcatcgtcg
      361 gtattgggca aacccatcag tcgggcgacc tgcacgccgg caggcgtttg aaccaaggtt
      421 tcgggcgtac cgcattgaag gattttcccc tcgtgcatga cggcgatttc gtccgccgcc
      481 gtgcaggcct cttcgggcga atgcgttacc aaaacggcag ggatgccgcc cttgcggatg
      541 cgttcggcgg tcatgcggcg cagccggtcg cgcaaatgcg tgtccaaact ggaaaacgat
      601 tcgtccagca gcagcaggga agggcggaca accaaagcgc gcgccagtgc caaccgttgt
      661 ttctcgcctc cggaaagttt ttcaggcttg cggtgcacct cgttttccag tccgacttcg
      721 gcaagtgccg ccatggcaag gcgttcggct tcggctttcg gcattttttg cattttcaaa
      781 ccgaatgccg cattttccag cgcactcata tggggaaaca gcgcgtaatc ttgaaacatc
      841 agagaaatac ggcgtttttc gggcggcata cgggtaatgt tttccccgtt cagctgaatt
      901 tcgccgccgt ccggccggac gatgcccgca atcatattca gcagggtgga ttttccgcag
      961 cccgaccgcc ccaaaacggc gagtattttg ccgcgcccga cagtcaggca gatgttgtcg
     1021 gcgacggttt tatcgccgaa gcgtttgcag agtccgttca gttcaagcat ggcgcatcct
     1081 ataaacgtat gccgtccagc cactcggaca gcggatgggc ggcatcaatc agaccgtaac
     1141 ggcaaaacgc gtccagcgta accagttgcg cgtcgtgcat catgtttccc gacaacatgg
     1201 cagccaacag accgccgatg tccattttct caaaacccgc cacttcgcca tcctgatttt
     1261 caggcaggaa ggtttcgggc aggacggcat cgaatacata caggatttca ttgtgcacac
     1321 cccggctgac ggggcgcagg ctgtgcagct gcgataccgg gcggatgagc ggaagcagcg
     1381 ttttatccaa accggcttct tcgctgcttt cgcgacacac ggtttcagac ggcaattcac
     1441 cgctggaaac accgccggcg gcagtattgt cgagtttgtc gggatcgact gctttgtgcg
     1501 gactgcgcct gcctatccag aaatgccatc ggccgtccga ttcggtcagt ccgttgagat
     1561 ggacggcgcg gctgagcagt ccgaacggac ggaaagcggc gcgttcgagc gtgaacaagg
     1621 ggttgccgcc gccgtcggtc aggtcgaaac actcgttgcg ccagccgtcc aacagccccg
     1681 cacagtgcca accgagggcg aggtgctgta agcgtccgcc catatccggc cagccgtccg
     1741 cattcaggaa aatgccgtct gaagactccg agcagcctgc ctcccagtct tttttgacgc
     1801 gttccaccca ttccggcgac aggttgccca aaggcaggcc gttcagatac agcgttttcc
     1861 agcaactttc tgcaccgtaa cttgcttttg cccactcgaa cagggcatca aggtcttgtt
     1921 tgctgacgga ttcggtaaaa cggacggtcg gcatggttgg ctttcttgaa gacaatgcgg
     1981 aagattgtag ccaaactgcc tgaatttgca accgcaaaca gacggcgggc tgccgttccg
     2041 atattgcggc aggaaggaaa atccggcaaa aaacggaggc ggcaggaaac aggcaaaaca
     2101 aaacccctcg gcattgaacc gaggggtttg atatttggtt gcgggagcag gattcgaacc
     2161 tacgaccttc gggttatgag cccgacgagc taccatgctg ctccatcccg cgtcagaaaa
     2221 cgaaactata cggcagattt tttatgttgt caaacattac ttccgcgaaa aatcataaaa
     2281 ttttgccgtc cggtggttta tgcctgattt gaaatattat tttctttaca aaagttcatg
     2341 ttcgtgattt aattttggtt aacattgaaa caggggtgct gcatgatgtt caggcggctg
     2401 agaaataccc tttacacccg atctggataa tacctgcgtg gggagttttc acggattctg
     2461 cttttcagac ggcattggtt ttcaaatgcc gtctgaaaac gcaaaacgct cctgtttctt
     2521 tattctaaac gagaaaacag gagcattttt tatgactacg ccaaaaaaaa ctgccaaaac
     2581 ttccggcaac gaagcgcgcg agcttgccga cttgagcgaa gacatcggca tccgctttaa
     2641 atatccgaac tcggagcgcg tgtatctgca aggcagccgc gacgacatcc gcgtgccttt
     2701 gcgcgaaatc cgtcaggacg acacctacac ggcgcaaggc acggaagcca acccgccgat
     2761 tcccgtctat gacaccagcg gcgtgtacgg cgatccggcg gcgcacatcg acctgaaaca
     2821 aggcctgccg cacatccgca ctgcatggct gaacgaacgc ggtgataccg aaatcctgcc
     2881 caagctttcc agcgaatacg gcatcgaacg cgcacacgat ccgaaaaccg cccatctgcg
     2941 cttcaaccaa atcacccgcc cgcgccgcgc caaagccggc agcaacgtaa cccagcttca
     3001 ctacgcgcgc caaggcatta tcacgcccga aatggagttt gtcgccatac gcgaacgttt
     3061 aaaattagac gaattgtccc aaaaatcgga atacgccaaa ctcttgaaac aacacgcagg
     3121 gcaaagtttc ggcgcgaaca tcccaacccg tcccgaccaa atcacgcccg aattcgtgcg
     3181 ccaagaaatc gccgccggac gcgcgattat ccccgccaac atcaaccacc ccgaactcga
     3241 accgatgatt atcggccgca acttccgtgt caaaatcaac ggcaacttgg gcaattccgc
     3301 cgtcacttcc agcctgaccg aagaagtcga aaaaatggtg tggtcgctgc gttggggcgc
     3361 ggacacaatt atggacttat ccacaggcgc gcacatccat gaaacgcgcg aatggattat
     3421 ccgcaacgcg cccgtcccca tcggcaccgt gcccatctat caggctttgg aaaaaaccgg
     3481 cggcattgcc gaagatttga cttgggattt gttccgcgac accttaatcg agcaggcgga
     3541 gcaaggcgtg gactatttca ccatacacgc gggcgtgttg ctgcgttatg tgccgatgac
     3601 cgccaaccgc ctcaccggca tcgtctcgcg cggcggttcg attatggcga aatggtgttt
     3661 ggcacatcat cgggaaaatt tcctctacac gcatttcgac gaaatctgcg aaattatgaa
     3721 agcgtatgac gtatcgttca gcctcggcga cggcctgcgc cccggctgca ttgccgatgc
     3781 caacgacgaa tcccaattcg ccgaactgca caccttgggc gaattgaccg ataaagcgtg
     3841 gaaacatgac gtacaagtca tgatcgaagg ccccggccat gtgccgctgc aacgcgtcaa
     3901 agaaaacatg accgaagagc tgcaacactg ctttgaagca cctttttaca cgctcggccc
     3961 gctcgttacc gacatcgcac ccggctacga ccacatcacc tcgggcatag gcgcggccaa
     4021 tatcggctgg tacggcacgg cgatgctttg ttacgttacc cccaaagaac atttggggct
     4081 gcccgacaaa gaagacgtgc gtaccggcat catcacctac aaactcgccg cccacgccgc
     4141 cgatctcgcc aaaggctggc cgggcgcaca attacgtgac aacgccctga gcaaagcgcg
     4201 tttcgagttc cgctggcgcg accaattccg cctcagcctc gaccctgaac gtgccgagag
     4261 cttccacgac gaaaccctgc ctgccgaagg cgcgaaaatc gcccacttct gctcaatgtg
     4321 cggccccaaa ttctgctcga tgaaaatcac gcaggaagtg cgcgactacg ccgacaagca
     4381 aaaagcccaa cggcagggca tggaggaaaa agcggtcgag ttcgtcaaaa aaggcgcgaa
     4441 gatttacagt tgaaacgtca agcaaaaaat gccgtctgaa acccggaaaa aaggcttcag
     4501 acggtattct ttcgcttgtg
//
LOCUS       DAXHRI010000154         4476 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXHRI010000154 DAXHRI010000000
VERSION     DAXHRI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.154"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..373
                     /locus_tag="ACFM75_001807"
     CDS             <1..373
                     /locus_tag="ACFM75_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC6256309.1"
                     /translation="QISGKRYQRLSLVSAQVGNRLIAPMVYQNTMTGVFFEAWFQQCL
                     LPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPLAPYSPELNPIEKVWANIKRYL
                     RTVLSDYARFDDALLSYFDFN"
     gene            complement(537..1940)
                     /locus_tag="ACFM75_001808"
     CDS             complement(537..1940)
                     /locus_tag="ACFM75_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC6256310.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(2009..3436)
                     /locus_tag="ACFM75_001809"
     CDS             complement(2009..3436)
                     /locus_tag="ACFM75_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC6256311.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYCRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3672..4025
                     /locus_tag="ACFM75_001810"
     CDS             3672..4025
                     /locus_tag="ACFM75_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256312.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            4022..4462
                     /locus_tag="ACFM75_001811"
     CDS             4022..4462
                     /locus_tag="ACFM75_001811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256313.1"
                     /translation="MKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFSDG
                     EYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHPARYAVTFENNVDSKLRRHWVAGAT
                     IRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAILCNKER"
BASE COUNT         1064 a         1169 c         1102 g         1141 t
ORIGIN      
        1 gcagataagt ggaaaaagat accaacgctt atctttggtg tccgcacaag tcggcaaccg
       61 gctgattgct ccgatggttt atcaaaatac gatgaccgga gtcttttttg aagcgtggtt
      121 tcagcaatgc ctactgcccg cattgactca aaaatcggta attattttag ataatgcgcg
      181 atttcaccgt atgggtgtct tacgggaaat ggcggaaaaa ttgggacata aggtattgcc
      241 tcttgcacct tattcacctg agctcaaccc gattgagaaa gtgtgggcga atattaagcg
      301 gtatctgcga accgttttgt ctgattacgc ccgatttgac gatgcactac tgtcctattt
      361 tgattttaat tgaatataca tttgaattaa ttcgcactta atttaaatgt gtttttaact
      421 gtgctttatt taaaggcaat gagaatgtga aagtatcgga tcaatcccaa agcagcctgc
      481 actttcgaaa cggggtgcag gctgctttgg gaatttcata accgtttcag cctgctttat
      541 tccgcaaata ccgtttccaa ccctaacccg ctctctttca ccaagcgcaa ataagccagc
      601 atgaatttat accgtgcttg agccagtttc tgttctgctt gggcgacttc ctgccgcgcc
      661 cgtattactt ccagccggtt gcggatgccg tattgttggc cggtttcggt cgatttcagt
      721 ttcaaacggc tgctttccaa aacccgttct tgcgccatga tttggtaacg cgccgcaccg
      781 ctttcggtat aagcctggcg tacggcgagt ttgatgtgcc gctcggttgc ggtcagctgt
      841 gcttcggcgg ccccgtattg cgcttcggct tcatggattt tgcccgacaa ttctccgccg
      901 gtataaagcg gcaaattcaa ctgtacgccg acgctcatcc ctttgccccg atagtggtag
      961 tcattattct gcgcagatga agtgtagagg ttattctgat agccgacatg ggcagaaacg
     1021 gtgggatagc ggctgttctg tgctgcccga agcgcctgtc cgctgctttg cagggcaagc
     1081 tgctgcatcc ggtattcatg attgttggat aaggcaatgc gctgccattc atccagactg
     1141 taacgttcca gcttgggcag atagcgtgcc aacaggttgg cggtatctat ggcctcgatt
     1201 tgtttgctat ccaggtcggt gtagtcgttc aactggtttt cataggtttg tttctcagcc
     1261 aatacggcga tttcttgggc cagggcattg tcgtaaccgg ctttggcttc gtgaatatcc
     1321 agcgcggtgg cagcaccttt attgaataaa gcctgcgcct gccttacctg ctgggcataa
     1381 gcctcttttt ccgccgcatg ggcggcaacg gtgtctcggc tgagtaaaac gttgaaataa
     1441 ctttcggcaa ctttcaacag caattcttcg cgtgccgcat cgaaacgctg ttctgcagcc
     1501 tgcgtatcga acctgctttg gcggtattgt gcaaatttgg cagcgtcaaa taaggtttgt
     1561 cccacctgca cgctccatcc ctgtgtttcg cgggtggaag aaatcgatgg cggctggcgc
     1621 tggtagctgg cattggcgga tacatgggga aggaatgcgg ccttggcttg ttgttgccgt
     1681 gcgcgcactg catcacgctg gtaatgggac gcttgaaaat cagccgaatg ttgctgcgcc
     1741 gcccgccatg cttcaggcag cgtaaaagcc gaaacggatg gggaaaggga tagtggcaag
     1801 gtaaaaagtg aaacgggtag gatatatttg gaaaaatagg atttcatagc cgaaaatagt
     1861 tcatgttgca aatagggcgt cagtgtcagg caaacggaaa taccgtaatc ttgcattatc
     1921 attagattga gcaatgtcat ccgggcaatg gtttcaggca gtctgcatgt ccgaaccggc
     1981 ggataacaaa tgcccagtac ggatccgcct atcgctccct aaagctttcg tccaatttgg
     2041 tttgcagcgg gcttaacaga taatccagca cccgccgttt acccgtttta atctccgccg
     2101 tgacattcat gcccgccgtc agattcactg ctttgccgtc aatattcaag gtatgtttgt
     2161 ccagcgacac caccgccgta taaaccaagc ccaactgttc gtggcttacc gcatcatggc
     2221 tgacactttt caccttgccc gtcagataac cgtagcgcgt atagggaaag ctctcaatct
     2281 tcaccaccgc atcctgtccc tgttccacaa aaccgatgtc tttgttcaat accaaaactt
     2341 ccacgtccat tttgtcgtca tcgggcgcaa tcaccatcat tttttgggca gcctgcacca
     2401 caccgcccac cgtataggtg gccaattcct gcaccgtgcc gtccgcaggc gactgtattg
     2461 tcatcagctg ctgccgctgc tttgccttat ccgtttggcc gcggtattgg tcaatctgtt
     2521 cgtttgcctg gcgcagcgca tccagcgtat cgcgtttcag gttctgcgta ttcagcaccc
     2581 gattctgctc cgcctgtgca atggccgcct gaatctgcct catctgaccg cgcgtacttt
     2641 ccaaatcgtt ccaattgctg accgatttgc tctgctgctc caaaaacgca tgttccgaaa
     2701 taaaattgtc ggcccgcaaa cggcagtagt ctgctgtttt ctgctgctcg atcgccccca
     2761 ccgaaaccag cttctgctcc tgcgccttgg ccgactgcaa ttccgcctga tggccgcgca
     2821 aagccgactg caattgcgca tcctgcgccg cccatgcctg atactggtgc tgcgccaaca
     2881 cctgcgccga ttgcacatcg gcatcggaga gacctaaaga ccgtgcttgc gccatatcga
     2941 tatgcggcac ggtacggctt tccaatgccg ccaataccgc ttcataacgc agtttggaca
     3001 attgggcagc ctgcaaagcc tgctccgact gcaccacatc gctgtctgtt cccacagcct
     3061 ccagttccgc cagcgtttct ccctgtttca catgctgccc gtcgcgcaca tgtaccgcct
     3121 taaccaccgc cgtttccagc ggctggatgg ttttgctgcg cccgcccgac accgttttgc
     3181 ccgaagccgc cgccacaata tcgattttgc cgaaccagga ccacaacaaa gccaaaagcg
     3241 caaacgccat aataaaacgc gccgcccatt tcggagcggc agagaccggc gtatcggtca
     3301 gttccaaatg cgcgggcaaa aacgcctgtt cttccgccgt gcgtttgggc ggtttcaact
     3361 ggtcgcgcac cgcccaaaca ttgcgccata cagtaatgta tcgagaaaga aaggatttca
     3421 gggcggagaa aaacataacg ggtataacct tggcaatata gaaacaggaa acaatataaa
     3481 tatgtaaagg aattttaacg gaaagcgcgg cagctgttaa gggaaaggcg ggaatattga
     3541 caaaaaatac ccaagtcgtt acaaatattc attattttac tgcgtaacgc aacgctgaag
     3601 cgcaggctgc ttttgagatg cggcaaggtt cggcaaaaag cagcctgcac atttaaccac
     3661 aggaacaacc catgtttacc acaaacgatt tacgccattt cctagaaggt ttggccatcc
     3721 tattctcaat cggctattgg ggcaccatgc tgctgttgct ttggtttctc gtccgctttg
     3781 cctataaaaa gcccaaacgg aaccccggca aaatcctttc aggcagcctg accgccgccg
     3841 ctatcctgat gctgtttgtt tggattattc ccaaacaatt cggcccgatc aaagaagaaa
     3901 tacaggcaca agaagagtgg gacagaaaat acaaagaagc cgaagccgtg tttaacgaac
     3961 aatgcaaaac ggcgggggaa agatttacca gacggcggac aatgtggaag ggattatgct
     4021 gttgaaggta gtacctgagc gtaccgtttc ggcagatgca aaaaccagag acccgatgtg
     4081 ggacaatgcg gctttacaga ccagcgaagg cgtaaatttt attgctcgtt tcctaggatt
     4141 ttttagcgat ggggaatacc gctatgtgga tgtcctgcaa cccaaccatt ccgatattat
     4201 tcggtattca ggtaaagatt ttccgataaa tcaaatactt aatcatatac accccgcccg
     4261 ttatgcggta acgttcgaaa acaatgtcga ttccaagctg cgcaggcact gggtggcagg
     4321 tgcgaccata cggattatcg accgccaaac tgacgaagtg attgccaaga aaaccatcta
     4381 tgtctttgaa aaaggcttgg acggcacggg tggggcgaga atgccgtgga agtttgctat
     4441 cttgtgcaat aaagaaagat agtgtatttc acggtc
//
LOCUS       DAXHRI010000155         4455 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXHRI010000155 DAXHRI010000000
VERSION     DAXHRI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.155"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(6..81)
                     /locus_tag="ACFM75_001812"
     tRNA            complement(6..81)
                     /locus_tag="ACFM75_001812"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Val,seq:tac)
     gene            complement(151..2127)
                     /locus_tag="ACFM75_001813"
     CDS             complement(151..2127)
                     /locus_tag="ACFM75_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC6256314.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2276..3115
                     /locus_tag="ACFM75_001814"
     CDS             2276..3115
                     /locus_tag="ACFM75_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC6256315.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALAQIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQDAAQN
                     TAS"
     gene            3316..4347
                     /gene="gap"
                     /locus_tag="ACFM75_001815"
     CDS             3316..4347
                     /gene="gap"
                     /locus_tag="ACFM75_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6256316.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1135 a         1487 c         1030 g          803 t
ORIGIN      
        1 aaacctggtg ggtgatgacg gagtcgaacc gccgacattc tgcttgtaag gcagacgctc
       61 taccaactga gctaatcacc cttgcgattt gcgaaagcgt tattaaacca aataaccgga
      121 aatggcgcaa gccttatttc ggcatttcat ttatattccc tgcaaactcc agttttcaaa
      181 agagataatt tcttcttttt tgcctgcgac caccaacacg tcaccgcctt ccaatgtaaa
      241 cgaggcatcc gggttttcaa tccggccggt gcggcggcgg acgaacaaga gtttgatgcc
      301 gtaagccgcc atcggaagcg tgccgactgt tttgccgacg gcgtatgctt ctgcagccaa
      361 aggaaaggcg tgacggacgg tttcgccgtt ttcgccgaat cctgcctcat catcactacc
      421 gacaaacagt ccctccaacg cggcataacg gctgtggcgg atatttgcca tcgtctgata
      481 gacgtgccga tacgacgcgc cgttgcctaa catcgcatag cctgccagca cgagtccggt
      541 ttctttggtg tccgacacgg cttcttccgc acctatatcg gtaaacgttt tcacataatc
      601 gtcgttggtg gcgcgcacat atacgggcat attgggatac atggacagca cattgtctaa
      661 aacgtgttgc gtttcgtgca tattgttgag cgtaaccacc accattttcg cccgtcccag
      721 accggcggct tccaatactt ccctgcgttt cgcatcgccg aacgacaccg gttcgcccgc
      781 acttctggca acctgcaccc gcgcaatgtc caagtcgagc gcgaaatacg gaatatcctc
      841 ttgggcaagg acgcgtccga ccgtctgccc gcccctgccg aagccgacaa tcagcacatg
      901 gtcggacttg ctcatggttt ctaccagcat actgtgcaga tcgagcgact tcatgtccca
      961 gcttgacttg accaaacgcc cgaccagcgc atcgctgccg cccaagagga agggcgcgat
     1021 aatcatcgac agcagaaccg ccgctgtcgc cgcctgttcc cattctggcg aaaccatatc
     1081 aagctgcccg gcaatggcca gcatcacgaa gccgaactcg ccgccctgcg cgagatacaa
     1141 agccgttttg aggctgtcgc cgaccgaatg tttcattttg aaggcaatgg caaacacaac
     1201 cagtgccttc aacaccagca gcattgccaa cagcatcaat acctgccgcc agccgccgat
     1261 caatgcctga atgtccagct tcatgccgac cgtgataaag aaaaagccga gcaaaatatc
     1321 gcggaacggg cggatgtcgt cttcgacttg gaaacggtat tccgtttccg aaagcagcat
     1381 gccggcaacg aatgcgccca acgccataga caaaccttcc agctcagtca gataagccac
     1441 acccaaggtt accagcagca cattgatcat aaagagttcg gacgatttgc gttttgccac
     1501 catcctgaac catcgcgaca taattttgct gccgacgaaa aacagcagcc ccagcgtcag
     1561 cagcattttt gcaaacgcca aacccaaggc cgcccaaata tttccgtccc ctccgcccgc
     1621 cagcgcggga atcagaatca tcagcggcac gacggcgatg tcctgcatca gcagcacgcc
     1681 catcgccatc tgaccgtgcg gctgccccaa ttccgtcttt tccgacaaaa tccggctcac
     1741 aatcgccgtg gacgacatcg ccaacgcgcc cgacacggca aacgcccaat tgaacggcac
     1801 gcccgtcagc atcagtatgc ccattaccga cagcatcgta acgccgacct gcaaaccgcc
     1861 cagcccgaac accagccgcc tcatcgccct caacttgggc agcgagaact ccaaaccgat
     1921 gctgaacatc aggaacacaa tcccgatttc acccaaataa tccgtcgcat ggcttttcgg
     1981 aatcaggctg agcataccgg gccccgccaa aaagcccacc agcaggtagc ccagcatgga
     2041 gggaatgttg aacttgcggc acaggatcac cgtaatgacc gacaccagca aaacaaccac
     2101 aataggggca agcgaaaatt cgttcatagg tcgtccgaac aggaaaatac agaaaaatgc
     2161 cgtctgaaac ggcatacgcc gccgtattat aacaaaacac cgcgcaccat ccgaaacggg
     2221 cgcggcatac aattctgcta aaatacgccc tttcgatttt gagccgcaca ccgacatgac
     2281 cgccacagcc gccgccatag accgcctgct tccccaaacc caatgccgcg aatgcggcta
     2341 cgacggctgc ctgccctacg cacaggcagt cgcaacaggc gaagcgtaca acctctgcgc
     2401 cccgggcgga gaagccgtca ttcgggacat ttccgccctg ctcggcaaac cccttgtcgc
     2461 acctgccaaa acccaagcca aagcactcgc ccagatagac gaaaccgcct gtatcggctg
     2521 caccgcctgc atccgcgcct gccctgccga tgccattatg ggcgcgggca aacttatgca
     2581 caccgtcatc gccgacgaat gcaccggctg cggactctgc gtcgccccct gccccgtcga
     2641 ctgcatccat atgcagcccg ttgccgacac cgtcctgccc cgcgcgcgtc gcttcagcct
     2701 gtccgccgac agccgttttg ccgccgccga acacgcgcgc gcgcgctacc tcaaacgcaa
     2761 cgaacgcaaa cagcgcgaag ccgacgaacg caaggccatg cttgccgaac gcgaagccgc
     2821 cgtccgcaac gcgcgtccgc aaacacccga cacaccgaaa aaaccggcgt ttaaccctgc
     2881 cgacctcatc gccaaagcca tggcaaaagc gcaaacccaa caagaccgcc tcgccgccgc
     2941 cgacaaccgc aaagactatc aggcgaaaca gatagccgaa gcccgcgaac gcgccgagtt
     3001 gcgccgcgcc caacgcgata tgaaatacgg cagcgacagc gaaaaagccg ccgccctcga
     3061 atacctcaaa caatacaaag ccaagcagga tgccgcacaa aacaccgcct cctgaccctt
     3121 ccccgaaatg ccgtctgaaa accgtttcag acggcatttt atcaagctct ccgtccgccg
     3181 cacccgtgcc gtccgcatct taccgcccac ccttccggcc gcgccgtttt caataaaata
     3241 ttaattacac gccactacaa atttgctata atccgccccg aaaatctacc caacccctca
     3301 acaaaggaac aaaccatggg catcaaagtc gccatcaacg gctacggacg catcggccgc
     3361 caggttttgc gcgccatcta cgattatcag attcaagacc aactccaaat cgtcgccgtc
     3421 aacgccagcg gcagccttga aaccaacgcc cacctcacca aattcgacac cgtgcacgga
     3481 cgctttgaag ccgacgtatc ccacgacggc ggcaacctca tcgtcaacgg cgacaaaatc
     3541 cccttcttct cgacccgcaa ccctgccgaa ctgccgtgga aagaactcgg tgtcgatttg
     3601 gtcatggaat gcaccggcgc gttcaccagc aaagaaaaag ccaaagttca tctggaaagc
     3661 ggcgcgaaaa aagtcctcat ttccgcaccg ggcggcgacg atgtcgatgc aaccgtcgtg
     3721 tacggcgtga acgacagcgt cctgaccgcc gacatgaccg tcgtttccaa cgcttcctgc
     3781 accaccaact gcctctcgcc ggttgccaaa gtgttgagcg aaagcgtcgg catcgtcaaa
     3841 ggcgcgatga ccaccatcca cgcgctgacc aacgaccaaa ccgttaccga cgtgcgccac
     3901 aaagacctgc gccgcgcccg cagcggcgtg gaaaacatga ttccgaccaa aactggcgcg
     3961 gcaaaagccg tcggtttggt actgcccgaa ttgaaagaca aactcgacgg gcttgccatc
     4021 cgcgtgccga ccgtcaacgt atcattggta gatttgaact tccaagccgc gcgcgacacg
     4081 acaatcgaag aaatcaacgc actgatgaaa gccgcctcgg aagcaggcgc gctcaaaggc
     4141 gttttaggct acaacaccct gcccttggtt tccatggact tcaaccacac taccgaagcc
     4201 agccacttcg acgcaacact gaccaaagtc gttgacggca acatgattaa agtcttcgca
     4261 tggtatgaca acgaatgggg cttcagctgc caaatgctga acaccgcacg ccgtatgttc
     4321 ggacttgaag tgcgcccgct caaataagca ataaaccgtc aaacaaaatg ccgtctgaaa
     4381 cccgatgttt tcaagttcca gacggcattt ttcatttttc accgtgattt tacccggctg
     4441 tcgtcatttc taatt
//
LOCUS       DAXHRI010000156         4409 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXHRI010000156 DAXHRI010000000
VERSION     DAXHRI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.156"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            176..2026
                     /gene="ltgA"
                     /locus_tag="ACFM75_001816"
     CDS             176..2026
                     /gene="ltgA"
                     /locus_tag="ACFM75_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC6256317.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPADKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENASDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNNAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            2315..2527
                     /gene="rpsU"
                     /locus_tag="ACFM75_001817"
     CDS             2315..2527
                     /gene="rpsU"
                     /locus_tag="ACFM75_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC6256318.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2659..3105
                     /locus_tag="ACFM75_001818"
     CDS             2659..3105
                     /locus_tag="ACFM75_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC6256319.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            3126..3275
                     /locus_tag="ACFM75_001819"
     CDS             3126..3275
                     /locus_tag="ACFM75_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256320.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQRGFDTQKQVLPINGFRLRPI
                     GQNAV"
     gene            complement(3304..3696)
                     /locus_tag="ACFM75_001820"
     CDS             complement(3304..3696)
                     /locus_tag="ACFM75_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC6256321.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3768..4373)
                     /locus_tag="ACFM75_001821"
     CDS             complement(3768..4373)
                     /locus_tag="ACFM75_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC6256322.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         1080 a         1246 c         1220 g          863 t
ORIGIN      
        1 gggcaaaggc agaggttcgc accaaaacgg caaatgattg aaaaatataa attatgtcgg
       61 aaacattcca aagccggtgc agtttcggca tataattcgg gcaaacgcct gttcagacgg
      121 cattttgtct tttccaaccc tgaccgttca gggttccgat ccttaaggaa atccgatgta
      181 cctaccctct atgaagcatt ccctgccgct gctggcggcc ctggtgcttg ccgcgtgttc
      241 ttcgacaaac acactgccag ccgacaagac cccggcagac aatatagaaa ctgccgacct
      301 ttcggcaagc gttcccaccc gccctgccga acccgaagga aaaacgttgg cagattacgg
      361 cggctacccg tccgcactgg atgcagtgaa acagaaaaac gatgccgccg tcgccgccta
      421 tttggaaaac gccagcgaca gtgcgatggc ggaaaatgtc cgcaacgagt ggctgaagtc
      481 tttgggcgca cgcaaacagt ggacgctgtt tgcacaggaa tacgccaaac tcgaacctga
      541 agggcgcgcc caagaagtcg aatgctacgc cgattcgagc cgcaacgact atacgcgtgc
      601 cgccgaactg gtcaaaaata cgggcaaact gccttcgggc tgcaccaaac tgttggaaca
      661 ggcagccgca tccggcttgt tggacggcaa caatgcctgg aggcgcgttc gcggactgct
      721 ggccggccgc caaaccacag acgcacgcaa ccttgccgcc gcattgggca gcccgtttga
      781 cggcggtaca caaggttcgc gcgaatatgc cctgttgaac gtcatcggca aagaagcacg
      841 caaatcgccg aatgccgccg ccctgctgtc cgaaatggaa agcggtttaa gcctcgaaca
      901 acgcagtttc gcgtggggcg tattggggca ttatcagtcg caaaacctca atgtgcctgc
      961 cgccttggac tattacggca aggttgccga ccgccgccaa ctgaccgacg accaaatcga
     1021 gtggtacgcc cgcgccgcct tgcgcgcccg acgttgggac gagctggcct ccgttatctc
     1081 gcatatgccc gaaaaactgc aaaaaagccc gacctggctc tactggctgg cacgcagccg
     1141 cgccgcaacg ggcaacacgc aagaggcgga aaaactttac aaacaggcgg cagcgacggg
     1201 caggaatttt tatgcggtgc tggcagggga agagttgggg cgcaaaatcg atacgcgcaa
     1261 caatgttccc gatgccgacg caaacagcgt cctccgtatg gcggaagacg gcgcgattaa
     1321 gcgcgcgctg gtgctgttcc gaaacagccg aaccgccggc gatgcgaaaa tgcgccgtca
     1381 ggctcaggcg gaatggcgtt tcgccacacg cggcttcgat gaagacaagc tgctgaccgc
     1441 cgcgcaaacc gcgttcgacc acggttttta cgatatggcg gtcaacagcg cggaacgcac
     1501 cgaccgcaaa ctcaactaca ccttgcgcta catttcgccg tttaaagaca cggtaatccg
     1561 ccacgcgcaa aatgttaatg tcgatccggc gtgggtttac gggctgattc gtcaggaaag
     1621 ccgcttcgtt atgggcgcgc aatcccgcgt aggcgcgcag gggctgatgc aggttatgcc
     1681 tgccaccgcg cgcgaaatcg ccggcaaaat cggtatggat gccgcacaac tttacaccgc
     1741 cgacggcaat atccgtatgg ggacgtggta tatggcggac accaaacgcc gcctgcaaaa
     1801 caacgaagtc ctcgccaccg caggctataa cgccggtccc ggcagggcgc gccgatggca
     1861 ggcggacacg cccctcgaag gcgcggtata tgccgaaacc atcccgtttt ccgaaacgcg
     1921 cgactatgtc aaaaaagtga tggccaatgc cgcctactac gccgccctct tcggcgcgcc
     1981 gcacatcccg ctcaaacagc gtatgggcat tgtccccgcc cgctgacgta ccgatgccgt
     2041 ctgaaacccg cccggtcttt cagacggcat ttttatcccg aacggcattg acggcgaacc
     2101 ataaatataa gacaatccga aaattgtttt tcctgctttt tcaagcagct tgacacggca
     2161 caagccgacc cgttaggagg tgatgtttcc gtcacggcgc gtatcccgcc gccgaaaggc
     2221 acagcgatac ggaaaaccta caacaccgtc tgccctaccc tttccaccga tatgatgggc
     2281 agatgcaaca accgaattta ttaaaggaaa taaaatgcct gcaatccgcg taaaagagaa
     2341 tgaaccattt gaagtcgcta tgcgccgttt caaacgcgcc gtagaaaaaa ccggcctgct
     2401 gaccgagctg cgcgcccgcg aagcctacga aaaaccgact accgaacgca aacgcaaaaa
     2461 agcggcagcc gtaaaacgcc tgcaaaaacg cctgcgcagc caacaactgc cgcccaaaat
     2521 gtactaaacg ttcaagtaca gattacaggt cagccctgtg atatgaggac acaccgcaag
     2581 acctgctctg cggtgtgttt tgcttttcag acggcatcga aacccgccgt ttccatccga
     2641 catcccagcg aggacattat gagcctgaaa atccgcctta ccgaagacat gaaaaccgcg
     2701 atgcgcgcca aagaccaagt ttccctcggc accatccgcc tcatcaacgc cgccgtcaaa
     2761 cagtttgaag tggacgaacg caccgaagcc gacgatgcca aaatcaccgc catcctgacc
     2821 aaaatggtca aacagcgaaa agacagcgcg aaaatctaca ctgaagccgg ccgccaggat
     2881 ttggcagaca aagaaaacgc cgaaatcgag gtactgcacc gctaccttcc ccaaatgctc
     2941 tctgccggcg aaatccgtac tgcggtcgaa gccgccgttg ccgaaaccgg cgcggcaggt
     3001 atggcggata tgggcaaagt gatgggcgta ttgaaaaccc gacttgccgg caaagccgat
     3061 atgggcgaag tcaacaaaat cctgaaagcc gtactgaccg cctgattgcc cgaatatcgg
     3121 acaaaatgcc gtctgaagcc cgtatcgcag gttcagacgg cattttcaat gtcccaatat
     3181 cgaatcggca ggggcaacgc ggtttcgata cgcagaaaca ggttttgccg ataaacggat
     3241 tccgtttgcg ccccatcgga caaaatgccg tctgaaacat gattccgttc agacggcata
     3301 gatttatttg accaatttca agcctttttt ggcgggccgg ggcgcggttt cggcagaggt
     3361 gttttcaggc ggcgtgtcgg ggcgatacgc ttccaattca aaccccatac cttcgccggt
     3421 ctcccgtgcg aaaaggctga ggacgtgtcc gacaggtatc catatatcgt gcgcctgtcc
     3481 gccgaagcgg gcggaaaaac tgatccaatc gttgtcgatt tgaaggtttt gcgtggcggt
     3541 cgcgccgatg ttgagcataa tttcgttgtc gcggacgtac tgcatgggga cgcgcgtgtg
     3601 ttcgttgacc cagacaagga tgtgcggtgt gaggctgttg tcgctgcacc attcgcagag
     3661 ggcgcggagg atgtagggtt tggtggaagt gggcataatg ggttccgtgt tgtacgccaa
     3721 aataggaaaa tgcctgcaaa acggtgggtt ttgcaagcat ttcggactta tttgcgcatg
     3781 gctttttcgg cgggtgtcag tgcttcgata aaggcttcgc gctggaagat gcgctcggcg
     3841 tatttgagca gcggcgcggc acttttgccc agtttgacat cgtagtggtc gagccgccac
     3901 agcagcggag caagggcgac atcaatcata gaaaaatctt cgccgaggat gtatttgctt
     3961 ttgctgaacg aaggggcaag catggtcaga ccgttgccga tggcttcgcg cgcttttgcc
     4021 tgttccttgt tggtggcggc ggggttttct aacacttgga cgtggttgaa caattctttt
     4081 tccatacggt acagcaccag ccggccccga ccgcgcataa cgggatcgcc gggcatcagc
     4141 tgcggatggg ggaagcgttc gtcaatgtat tcgttgatga tattggactc gtgcagcacc
     4201 aaatcgcgct cgaccagcac gggaacttgg ttatacggat tcatgacggc gaggtcttcg
     4261 ggtttgttgt aaatatcgac gtctttgatt tcaaaatcca tacctttttc gtacaaaacg
     4321 aagcggcagc ggtggctgaa ggggcaggta atgccggaat agagggtcat cataataatt
     4381 gtcgctcctg tgtgatgcct gcaaaacgg
//
LOCUS       DAXHRI010000157         4262 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXHRI010000157 DAXHRI010000000
VERSION     DAXHRI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.157"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            538..2265
                     /gene="ilvB"
                     /locus_tag="ACFM75_001822"
     CDS             538..2265
                     /gene="ilvB"
                     /locus_tag="ACFM75_001822"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC6256323.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            2276..2767
                     /gene="ilvN"
                     /locus_tag="ACFM75_001823"
     CDS             2276..2767
                     /gene="ilvN"
                     /locus_tag="ACFM75_001823"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC6256324.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2834..3127
                     /locus_tag="ACFM75_001824"
     CDS             2834..3127
                     /locus_tag="ACFM75_001824"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC6256325.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3207..4220
                     /gene="ilvC"
                     /locus_tag="ACFM75_001825"
     CDS             3207..4220
                     /gene="ilvC"
                     /locus_tag="ACFM75_001825"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC6256326.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1106 a         1022 c         1075 g         1059 t
ORIGIN      
        1 atcaatatat taagtcgttg ttagccctct tggttctgtc aatgccgtcc aatacagtcc
       61 ggtgcaatga gccattttca ggaagtatgg ttgggaatat ttttgatata tcaaaaataa
      121 aatcaagttc aatcaatatc ccgagtcttt attgggatga aaccctgtaa attttacaat
      181 tcttgatatc gaagaagggt ttttcacatc tgcccctcct acacaaactg tcaacaattt
      241 ccatctttca aaaaaggtac tcctgatttt attcttttaa aatcaatcaa taatttctct
      301 gctttcaaaa aatattaaaa gatgaaagat ttttcttgat tgaactgtcg gtttgcgtta
      361 tcgtttctac tctttttcac aaactctgtg ttcctttcca attgattgga taatccgtct
      421 gttgccgtgt tcccccaatg cgcgtaccct aaatcgctgc ttggtggaat tgcattacag
      481 gtgctgtggc aaagcggttt gtccctattg gtttgaaacc gtataaaaga ggtcgttatg
      541 cagttatcag gcgcgcaaat catagtgcag agtctcaaag ccgaaggtgt cgagtacgtt
      601 ttcggttatc ccggcggtgc ggttatcgaa atctacgatg cccttttcca actcaataaa
      661 ttcaaacaca ttcttacccg ccatgagcag gcggcagtac acgcggcaga tgcgtatgcg
      721 cgcgtcagcg gtaaggtggg cgtggcattg gttacatccg gcccgggcgt taccaatgca
      781 ctgaccggta ttgctactgc ctatacggat tcgattccga tggtggtcat cagcgggcag
      841 gtaggcaatt ccctgattgg tacggatgcg ttccaagaag ttgatacggt gggtattacc
      901 cgtccgtgcg tcaaacacaa tttcctggtt acggacatca atgagttggc ggaaaccatt
      961 aaaaaggcgt tccaaattgc cgcaagcggc cgaccggggc ccgtggtggt tgatgtcccg
     1021 aaagatgtta cgcaggcgat ggcgaaattc agctatcctc aggaagacat ttttatccgt
     1081 tcgtatcaac cggttgttca aggacatatc ggtcagatta aaaaggccgt gcagatgttg
     1141 gcatctgcca aacgtccggt cgtctatttt ggcggcggcg tggtgttggg taatgcttct
     1201 gaagagctga cccgatttgt ccgaatgacg ggtgctccgt gcacgggtac gctgatgggc
     1261 ttgggcgctt atccttccgg cgaccgccaa ttcctcggta tgctcggtat gcacggtact
     1321 tacgaggcaa accttgccat gcagaatgcg gatgtcgttc ttgccgtagg tgcgcgtttt
     1381 gacgaccgtg tggtttccgt accgtccaaa tttttcgaga aggcgaagaa ggttatccat
     1441 atcgatgtcg atccttccag catcgccaaa cgcgtgaagg tggacattcc gattgtcggc
     1501 gacgtgaaaa acgttttgtc cgagatggtt gcgctgtggc aaaaacaaga gtccgtgccg
     1561 tctgaagatg ctttgggcaa atggtggaaa accatagagg aatggcgttc ccgagattgc
     1621 ttgtggtttg acaacggcag cgaaattatc aagccccaat atgtgattca gaagcttgcc
     1681 gagattaccg gcaattcggc aatcatcaca tcggatgtag ggcagcatca aatgtttgcg
     1741 gctcaatatt atcccttcga acgtccgcgc caatggctca attccggcgg tttgggtacg
     1801 atgggcgtgg gtctgcctta tgcgattggt gcaaaacttg ccgccccgga tcaagacgta
     1861 ttctgtatta ccggcgacgg ctcgatccag atgaacatcc aagagctgtc cacctgtttc
     1921 caatatcgga ttccggttaa cgtcattacg ctgaacaacg gttatcttgg tatggtacgc
     1981 cagtggcagg aaatatatta cggcggtcga gagtcggaaa cctatttcga ttctttgccc
     2041 gatttcgtca aacttgccga ggcatacggc catatcggta tccgcgtgga caagaagtct
     2101 gatgtggagg gtgccttgtt ggaagcattg aaccaaaaag acaggctggt gtttatcgac
     2161 ttcctgactg accagaaaca gaatgtgatg cctatggtcg gcaacggcaa aggtttggac
     2221 gaaatggtac ttccgccgca tatgcgtgcg gacggaaagg cgtaaggaga gacaaatgcg
     2281 acatatctta tctgttctga tcgaaaacga atcaggtgcg atgagccgcg tggtcggttt
     2341 gttctctgca cgcgattaca atatcgattc tttggcggtt gcgccgaccg aagacaaaac
     2401 cctgtcccgt atgaccatcg ttacccacgg cgacgagcag gttatcgaac aaatcaccaa
     2461 gcaactcaat aaattgattg aagtgattaa agtggtcgat ttgaacgaaa gccgttttgt
     2521 cgaacgcgaa ctgatgttgg taaaagtccg tgccgccggc aaagaccgcg acgaattttt
     2581 acgcttgacc gaaatctacc ggggcagcat catcgacgta accgaccgca gctatacgat
     2641 tgaaattacc ggctcgaccg acaagctgga ttcctttttg gaaacggtcg gacgcgccca
     2701 aattttggaa accgtacgta caggcgcggc cggcatcggt cgcggtgagc gtattttgaa
     2761 aatttaacgc cgtaaccttt cagacggcac ggtatttgaa tgccgtctga aaaacgaacg
     2821 gcaggagaga tttatgtcga acattaaaat tgtcgcactg gttaccgtca aaccggaata
     2881 cacagaaaca ctggcagcac agtttaaaga actggtcaaa gccagccgtg cagaagaggg
     2941 caacatcagc tacgatctcc atcaggaaat cggcaaaccg aaccgttttg ttttcgtgga
     3001 aaattggaaa tctcaagcag ctattgacga acataacgcc agcgcgcatt tccaagcctt
     3061 cgtccagtcc gtcgacggca aaaccgaagc gttggaaatc gtattgatga atgaagttgc
     3121 cgattaagcg gcacactctg tttaacccgt ccgaagccgc tcaacatttt aaggcttcga
     3181 caaccattta ccttaaagga aatcaaatgc aagtctatta cgataaagat gccgatctgt
     3241 ccctaatcaa aggcaaaacc gttgccatca tcggttacgg ttcgcaaggt catgcccatg
     3301 ctgccaacct gaaagattcg ggtgtaaacg tggtgatcgg tctgcgccaa ggttcttctt
     3361 ggaaaaaagc cgaagcagcc ggccatgtcg tcaaaaccgt tgctgaagcg accaaagaag
     3421 ccgatgtcgt tatgctgctg ctgcctgacg aaaccatgcc tgccgtctat cacgccgaag
     3481 ttacagccaa tttgaaagaa ggcgcaacgc tggcatttgc acacggcttc aacgtgcact
     3541 acaaccaaat cgttccgcgt gccgacttgg acgtgattat ggttgccccc aaaggtccgg
     3601 gtcataccgt acgcagtgaa tacaaacgcg gcggcggcgt gccttctctg attgccgttt
     3661 accaagacaa ttccggcaaa gccaaagaca tcgccctgtc ttatgcggct gccaacggcg
     3721 gcaccaaagg cggtgtgatt gaaaccactt tccgcgaaga aaccgaaacc gatctgttcg
     3781 gcgaacaagc cgtattgtgc ggcggcgtgg tcgagttgat caaggcgggt tttgaaaccc
     3841 tgaccgaagc cggttacgcg cctgaaatgg cttacttcga atgtctgcac gaaatgaaac
     3901 tgatcgttga cctgattttc gaaggcggta ttgccaatat gaactactcc atttccaaca
     3961 atgcggagta cggcgaatac gttaccggcc ctgaagtggt caatgcttcc agcaaagaag
     4021 ccatgcgcaa tgccctgaaa cgcattcaaa ccggcgaata cgcaaaaatg tttatccaag
     4081 agggtaatgt caactacgcg tctatgactg cccgccgccg tctgaatgcc gaccaccaag
     4141 ttgaaaaagt cggcgcacaa ctgcgtgcca tgatgccttg gattactgcc aacaaattgg
     4201 ttgaccaaga taaaaactga ttgttttcaa acgggactgc ctacacatcg tgtaggcagt
     4261 tt
//
LOCUS       DAXHRI010000158         4245 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXHRI010000158 DAXHRI010000000
VERSION     DAXHRI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.158"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..480
                     /locus_tag="ACFM75_001826"
     CDS             <1..480
                     /locus_tag="ACFM75_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256327.1"
                     /translation="AAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRK
                     GAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVL
                     PDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            499..873
                     /locus_tag="ACFM75_001827"
     CDS             499..873
                     /locus_tag="ACFM75_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6256328.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            1258..2229
                     /locus_tag="ACFM75_001828"
                     /pseudo
     CDS             1258..2229
                     /locus_tag="ACFM75_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            2267..2932
                     /locus_tag="ACFM75_001829"
     CDS             2267..2932
                     /locus_tag="ACFM75_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6256329.1"
                     /translation="MSIKYIAMPAAWKYMPTDVSRVAKEVFGYLYNSQGASTRFDKYF
                     NTIDNHADFFAASNLYEQNLGSKARWFGGADFVSRAAITGLGADGEASYITFAAGKVV
                     GNPPIYEWRAASGNALIVNGFYNFRDLFNKKTNPREWDIQQLKSEQKLLQPIHQKYLS
                     NEKDYLSLIKGVTSNKIFSIIPNPLDERKKIEDGINMLDYKSRIKYGCKLMGYSEKQG
                     CKP"
     gene            2929..3363
                     /locus_tag="ACFM75_001830"
     CDS             2929..3363
                     /locus_tag="ACFM75_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256330.1"
                     /translation="MNKNYLFYLIQNIKSIFLIGGVFFGLLIFSGLWGNDFKLSFLGV
                     IDFIFGMVGGGFIVMLWFLIPVILSTLFRWVFQNQLAVSVILTFIVFYTYGYFSLFNS
                     SERVGSFWYLVIPTIMFIGLSEWFIYKSSVHSLGENVTPRSN"
     gene            complement(3431..3699)
                     /locus_tag="ACFM75_001831"
                     /pseudo
     CDS             complement(3431..3699)
                     /locus_tag="ACFM75_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244123.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            3698..3871
                     /locus_tag="ACFM75_001832"
     CDS             3698..3871
                     /locus_tag="ACFM75_001832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256331.1"
                     /translation="MIKQVANNLPDGSPAKAAVFKAEREGKLKTAIAGVDRQTGKAVI
                     LPVKVPSKANIRR"
     gene            3874..>4245
                     /locus_tag="ACFM75_001833"
     CDS             3874..>4245
                     /locus_tag="ACFM75_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6256332.1"
                     /translation="MGYNMMTTQKWHEHITDVIIGTTQRFNTEKSTDSIEYINERKGS
                     PIGAICIVSIDVYAACSHAYLFEHNLKKFKQYAYAYSKLEILASMGWSDPTPFFFPCD
                     MLNIQNPMFLMLMSDSPQLREF"
BASE COUNT         1313 a          788 c          962 g         1182 t
ORIGIN      
        1 gctgctccta aagaaacaat aaacaatttg gcaaatttag ccaaagcaga acagcagatt
       61 ttattccgta ttgcccaacg cgatacgcaa ctggatgcat ggaagacggg atttaacaat
      121 agagtaagga aaggagcagg cttgcttgat gcaagtaata ttccgataac cattaacgga
      181 aaaaccatca aacctgtaca agccataagc ttaaagggag cacccgttta cagcggcgta
      241 agcgaacagg agatttttgc gctttatcgg cagatgactg gccagaatcc gaattttaga
      301 gttttgcctg acggaagatt agcaaatggc attatcagta ctggagaatg ggcaggaaca
      361 aaaattgcat taagaaattt ttcaaaaaca gagaattcaa ctcaagcacg atggacatta
      421 gatttgcaga atcctccatc atttattaaa ggtactaaat tggagcttaa attccaataa
      481 tttacaaagg attttaccgt ggatgagaaa caaaaaatta agattcttga ttttcaaatc
      541 gatttatcct caatttttaa ctcttataaa aatcaaatgg gtattaatat tcaagataaa
      601 aacttaaaaa acaatttctg ttcttttatg gaagaactgt taaatgacgg ttcaatccgt
      661 ttacatgatt ataccgacgg tatcggaatt cctctaactg gaacttcaaa agaacaagtg
      721 cagaaattga aagacatatg gcctactttg gaagatgccc aagcaatatg gcctgaagac
      781 ccttggtatt acttagaatg gctttggtgg gatattgcgt gtccaataga tttggccgat
      841 ttgccgaata ttgatattta tgagcaagcg taggtatggt tagccgcctt tagcggcgta
      901 accgtacgca tatcagcaaa ctttataaaa taacaaggcc gtctgaaatc tgtttttcaa
      961 ctttttcaga cggccttgca acttggcatt tcattcgtac ggttacgccg ctaaaggcgg
     1021 ctaaccgtac ctacgagctc tgataaaaat gatttatgga agcaagctgt agcctgcatg
     1081 aaacctaaaa tccatgcgta aggtgtgtgc ttcagcgcgc acgcgttcca tgatttacgg
     1141 ctcaatgccg tctgaaaagc tcaatttttt cagacggcat ttgttatgca agtaaatatt
     1201 cagattccct atatactgcc cagatgcgtg cgtgctgaag acacccccta cgcttgctat
     1261 ttgaaacagc tccaagtcac caaagacgtc aactggaacc aagtacagct cgcttacgac
     1321 aaatgggact ataaacagga aggcctaacc ggagccggag ccgcaattat cgcactggcc
     1381 gttaccgtgg tcacctcagg cgcaggaacc ggagccgtat tgggattaaa cggtgcggcc
     1441 gccgccgcaa ccgatgcagc attcgcctct ttggccagcc aggcttccgt atcgttcatc
     1501 aacaacaaag gcgatgtcgg caaaaccctg aaagagctgg gcagaagcag cacggtgaaa
     1561 aatctggtgg ttgccgccgc taccgcaggc gtagccgaca aaatcggcgc ttcggcactg
     1621 aacaatgtca gcgataagca gtggatcaac aacctgaccg tcaacctagc caatgcgggc
     1681 agtgccgcac tgattaatac cgctgtcaac ggcggcagcc tgaaagacaa tctggaagcg
     1741 aatatccttg cggctttggt gaatactgcg catggagagg cagcaagtaa aatcaaacaa
     1801 ttggatcagc actacatagt ccacaagatt gcccatgcca tagcgggctg tgcggcagcg
     1861 gcggcgaata agggcaagtg tcaagacggt gcgataggag cggcggtcgg tgaaatcctc
     1921 ggcgaagccc tactggacgg cagagaccct ggcagcctga atgtgaagga cagggcaaaa
     1981 atcattgcta aggcgaagct ggcagcaggg acggttgcgg cgttgagcaa gggggatgtg
     2041 aatgctgcgg cgaatgcggc tgctgtggcg gtagagagta atgcgcttag caaggaaaga
     2101 atggataaat tgacaaaatg cctttccggt aaaacttgtt ctactacgat ggaaaaagta
     2161 aatgccatca aaaaggatga acaatttagc aaagtaattg atacggaaat tcaaaaaagt
     2221 ctgttctagg aacccattgg gcgatggttg cagaaacggc attaatatgt ctattaaata
     2281 tattgccatg cctgctgcgt ggaagtatat gcctacggat gtatcacggg ttgccaaaga
     2341 agtttttggc tatttatata actcacaagg ggcatctaca agatttgaca agtatttcaa
     2401 caccattgac aatcatgcag atttctttgc tgccagcaat ctttatgagc aaaatttggg
     2461 ttcaaaagca cgatggtttg gtggagctga ttttgtatcg cgtgctgcta taactgggtt
     2521 aggggcagac ggagaagctt cttatataac ttttgcggca ggtaaagttg ttggtaatcc
     2581 tccaatttat gaatggaggg ctgcgtcagg caatgcactg atagtaaatg gattttataa
     2641 ttttagagac ttgttcaata aaaaaactaa tcctagggag tgggatattc aacagttgaa
     2701 aagcgagcaa aagttattac agcctattca ccaaaaatat ttgagtaatg agaaggatta
     2761 tttgtcattg attaaagggg taacatcaaa taagatattt tcaataattc caaatccttt
     2821 agatgagagg aaaaaaatag aggatggaat taatatgtta gattacaaat ctagaattaa
     2881 atatggttgt aagcttatgg gatattctga gaaacaggga tgcaagccat gaataaaaat
     2941 tatttatttt atcttattca aaatattaaa tctattttct taataggtgg agtatttttt
     3001 ggccttttaa ttttttcagg tttatggggt aatgatttta aactatcttt tttaggggta
     3061 atagatttta tatttgggat ggtagggggt gggtttatag ttatgttatg gtttctaatt
     3121 ccagtgatat tatcaacttt attcagatgg gtatttcaaa atcaacttgc tgtatctgtc
     3181 atattaacct tcattgtttt ttatacatat gggtactttt ctttatttaa ttcatctgaa
     3241 agagtcggta gtttttggta tttggtaatc ccaaccataa tgtttattgg actgtctgaa
     3301 tggtttatct ataaatctag cgtgcatagt ttaggtgaaa atgtaacgcc acgttccaat
     3361 taaagaaaaa gattgtctga aaactaaatt taatttcaga tgaccttaga ttcggatttc
     3421 aagtacaaca ctagggtatc gatggttgga acagattcaa gaataaaaca cttggcgttt
     3481 cgtagccaag tgtttttctc ggtcggtggt tcaactcatc ttgaacccta cgtatctccc
     3541 gatcgctgat gtttcggaaa acggtttgtt tggggaatat tgtcggatga gtccattggt
     3601 gttttcattc agccctttct cccaagaatg gtaagggcgg taaaaatagg tttccgcctt
     3661 caatgttttg gccatttgga tgtgtcggta gaatgctttg attaaacaag ttgcaaataa
     3721 cttacctgat ggtagtcctg ctaaagctgc tgtcttcaaa gcagagcggg aaggcaaatt
     3781 aaaaacagca atagcaggcg ttgatcgtca aacaggtaag gccgttattc ttcctgtcaa
     3841 agttccttct aaagccaata taaggagata acaatgggat acaatatgat gaccacccaa
     3901 aaatggcatg agcatattac agatgtaatt ataggcacca ctcaaagatt taatacagag
     3961 aaaagtactg atagtataga gtatattaat gaaagaaaag gatctccaat tggagcaatc
     4021 tgtatcgttt ctatagatgt ttatgcggca tgctcacatg cttatctgtt tgaacacaac
     4081 cttaaaaagt tcaaacagta tgcttatgct tacagtaaac ttgagatact ggcaagtatg
     4141 ggatggtctg accccacgcc tttcttcttt ccctgtgaca tgctcaacat tcaaaatccg
     4201 atgtttctga tgctgatgag cgacagcccg cagctgcgcg agttt
//
LOCUS       DAXHRI010000159         4219 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXHRI010000159 DAXHRI010000000
VERSION     DAXHRI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.159"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(248..1111)
                     /gene="rpoH"
                     /locus_tag="ACFM75_001834"
     CDS             complement(248..1111)
                     /gene="rpoH"
                     /locus_tag="ACFM75_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC6256333.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLAEKAEAV"
     gene            complement(1375..2913)
                     /gene="lnt"
                     /locus_tag="ACFM75_001835"
     CDS             complement(1375..2913)
                     /gene="lnt"
                     /locus_tag="ACFM75_001835"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC6256334.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTTRSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(2982..3708)
                     /locus_tag="ACFM75_001836"
                     /pseudo
     CDS             complement(2982..3708)
                     /locus_tag="ACFM75_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            3902..>4219
                     /locus_tag="ACFM75_001837"
     CDS             3902..>4219
                     /locus_tag="ACFM75_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HFC6256335.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSARLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARSI"
BASE COUNT          912 a          965 c         1307 g         1035 t
ORIGIN      
        1 atttgtactg tctgcggctt cgccgccttg tcctgatttt tgttaatcca ctatatcaat
       61 tccgccaatc tgtcggaaaa gcagctgatg cggcagtgtc tggtgcatgt ctgctttttg
      121 atttcggcaa ttgcaacggc gtggacggat aaaatcgtgt acagcacgat acacaagcct
      181 cattggggtt tctattataa aaaaacaccc gacaaaccgg cttgtcgggt gtttaattat
      241 gtctatttca aaccgcttcg gctttttcgg caagaaaacc acgcagtttc tgcatagctt
      301 tcgcttcaat ctggcggatg cgctcggcag agacgccgta ttcggcggca agctggtgca
      361 gcgtcagccc gccgtcgtct tgaagccagc ggctttccac aatacggcgg ctcctgtcat
      421 ccagttgcgc caaagcgttt tgtaaacctt ctgtttgcag ggcgtaatgc gcctgtttcg
      481 atagttgtcg gctcggttcg gaatcgtggt cggcaagcca gtcgatgggg gcgaaactgt
      541 cctcgtcgtc gctgttgtct gccatgatgg cgatgtcgtg tcccgtcatg cgctgttcca
      601 tttccagaac ttcggaaagt ttgacaccca aatcgtcggc gatgtcttgt gcctctttgg
      661 gagacagggc gttgaggttt ttacgcatac tgcgtaaatt gaaaaacagc ttgcgttgcg
      721 gtttggtggt ggcaacgcga accaaacgcc agtttctcaa aataaactcg tggatttcgg
      781 ctttaatcca gtgtacggca aatgaaaaca gacgcgcgcc tctaccgggc tcgtagcgtt
      841 tgaccgcctt catcagtccg atattgcctt cctgaatcag gtctgcctga ttcagcccgt
      901 agccgtcata gccgcgcgcg atggaaacga cgacgcgcag gtgggacagg atgagttgtt
      961 tggcggcgtt gaggtcgccc tttatccggc gttcggcgag gcgggtttct tcttcctggg
     1021 acagcatcgg gatgctgttg acggtgtgga tgtattgttc gaggctgccg ttgccgcttt
     1081 ggatggcggg taatgcgaaa gcgttattca tttggggcat ttcctttcgg ctgaaactgc
     1141 gtatcggcgg tttgctgtgt tgggatgcag tatatcactg cttggcttgt attttgtatg
     1201 tttggcagga gatatgcgct aaggtttgaa agacaggaaa attttgtaag gcaggtttga
     1261 ttgattttgt aaacctgatg gctcaattcg atttcagaat tatattacat acgtaattgt
     1321 atgtaaatag ccggtttgaa aaaagacagc ccgtccggac gggctgtgca ggtatcagtg
     1381 ttctttgttt cggaagatga aaagaatcag tgcggctagg gtcaatatgc ccatcaacca
     1441 ccatgaactg ccggttttca tatagggcgt ttcgccgaca tagcctttga tgtgtccttc
     1501 caatacggtt tccgtatcgg gttgggcttg ggcgatgatg ttgcctttgg gggagatgat
     1561 ggcggttgcg ccggtgttgg tggcacggac catatagcgt ccgagttcca tagcccgcgc
     1621 ctgcgattgt tggaggtgct ggtacatggc gttggatttt ccgtaccacg ccatattgct
     1681 ggcattggca agcagtgtgg catctttggc ggcggcaatc agttcgtcgc cgaatccgtc
     1741 ttcgtaacag atgttgaagg cgattttttg gtttttcatc agcagggcgg attgcttacc
     1801 gccgcctttg cggaagtcgg aaaggggcat attcatcatt ttgtaaagcg gcgtggtcag
     1861 gaagggcagc ggtttgtatt cgccgaaggg gacgaggtgg tttttggcgt agtaggggat
     1921 gccgtcctga ttgttttcct gataaccggt caggttgatg acggcgtttt cgtaaccgtt
     1981 gccgtccgaa gtgtattggc tgatgccgac ggcgagcgcg ctgccgttgt tttgcgcctg
     2041 ttcggcaaat tttgccagta tgttttctgg caggttttgg cgcataacgg ggatggcggt
     2101 ttcgggcagg atgacgatgt cggcagtggt tttgccgacc tgttcgtaat atttctgtat
     2161 ggtcgggatg acttggtctt cacgccattt gagggtttgg tcgatgttgc cttgaagcag
     2221 ggcgacggtg ctgcggctgc cgtcggggcg ggtgaagtcg gtttgtcggg cggtgtagcc
     2281 tgcggcaagc agggcggcaa tcaggataat tggaagcagg cgtttgcccg aacgtgtggt
     2341 gttatcactc gccaaaacca gccagacacc gagaaaggcg gttgccagtg taaccatgtg
     2401 gatgccgccc aatggggcaa agccggcgag cgggctgtcc ggggtgattt gggagtagcc
     2461 gattgcgccc cagccgaatc cggtcaggaa acgttcgcgg gcaaactcgg tcagcgtcca
     2521 caggatgggc agtaccaaac cgatttttat gccccgaggc agggtaaatt ttttccatag
     2581 ccagaaacac agtgccggat aaagggcaag gtaggcgggg agtaggaagg tcagcggtac
     2641 ggcatagagg tcgggcaggc cggaaacgtc gtgcagggcg gtgtgtatcc agtagaactg
     2701 tgtcgtgtat gcggtcaggc cgaacaggta ggcggaagag acagcaaaac gcggacgcag
     2761 ttcgatgagg cggacgaagg caccgaaaat caagggcatc agccaaaagt ggtagtaggg
     2821 tgcgaaggta aagggggtgg cggcggcaaa aaggatgagc aaaggccagt agagggcggg
     2881 gtgctgccag tatttgtcca gtttggaaac catattcatc tgtctgttcg gaagataccg
     2941 tctgaacatc tttcaaacgg catcggtatt tgaaaaagaa atcaatgcct gccgaaacga
     3001 ttcatcagcg gcaaggcggg ggcgcaggca atcgaaccgg aaagcagccc gacaacgagg
     3061 ttggcgaagt actccgccca gccagcgtcc cagtgttgcg cgtgcaggaa atcgtgcaga
     3121 aggccgaggt tgtgggcgac cagtacgccg ccgataagaa acatggcaag cgtgccgacc
     3181 acgctcaaac cgcgcataaa gcaaggcata aaggcagtca gcatttgccc caaactgcgc
     3241 gaaaaggttt gtgggcggcg catcagcagc atgcctaagt cgtcgagttt gacgatgacg
     3301 gcaacgattc cgtacaccaa aacagtcatg ccgatgccga ttgccgccat tacgagcatg
     3361 cgcgtcatgt cggtgcagaa acttgtgcag cagcttttct acgccgtcaa agcacagata
     3421 aatgccgcct gccgtcaaaa gcggcgtaat gagttgcggc aggaaggcgg aaagcagcag
     3481 agccgcaggt accaaaaccg gcttgttgga aaaagaacct ttcgccatcg accaaacaat
     3541 cggcaactcg cgttctgccg atacgcccgt aacccggttg gcattgggtg ccaaatcgtc
     3601 gccgaccatg ccggcggttt tctttgcggc ggctttggtc atcagggcaa catcgtccaa
     3661 aacggcggtg atgtcgtccg gcagggtaaa tagtgaggca aatgccatta aagaatcctg
     3721 aaatgcggcg caaagtccga cattatatag gagaacgcgg atttgggcgg tttcaggcgg
     3781 catgaaacag gaaaatgccg tctgaaggcg gtggcggacg tgaagtaaag tttcgtgaaa
     3841 aaaatgccgt gttacagtct ttcgatttta atttaatgaa ttttaaggga gaatcgttag
     3901 cgtggattgg atgggcagtc tgttcctgcc gggtggcgca ctgttgttcc tgagcgtggt
     3961 ttcgaccact ttgtccgcac gtttgggaat gcctttgctg ctggtttctc ccgccggcgt
     4021 gttggatggg gcggcggaag ccttggcgat tgcggcgttc ctgatgttgg tcgcgcgtcc
     4081 gccggcagtg ttcggcggtt tgtggaaatt caattatagc ctgcgtgaaa aggcgtatag
     4141 ccgaatagaa atgcagtccg acaccgtgct tcaggcgggg atttggcgtg gtacatcctg
     4201 cccgacggca aggtcgata
//
LOCUS       DAXHRI010000160         4183 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXHRI010000160 DAXHRI010000000
VERSION     DAXHRI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.160"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>4183
                     /locus_tag="ACFM75_001838"
     CDS             <1..>4183
                     /locus_tag="ACFM75_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6256336.1"
                     /translation="SHAVKIDGPVWGKDVRVSAGKNNVSADGSIRSSHSPATNINSDN
                     SLYAIDTGALGGMYAGKITLISTDRDASVRNQGQWFASAGNVAVNAEGKLVNTGMIAA
                     TGENHAVSLHARNVHNSGTVASQDDANIHSQTLDNSGTVLSSGQLTVRNLGRLKNHNN
                     GTIQAARLDISTGGLDNTGNITQTGSQALDLVSAGKFDNSGKIGVSDVPQTGLNPNPS
                     VIPQIPSTATGSGSSTISASKPGSNNPVSPTAPAKNYAGGRIQTTGAFDNAGSINAGG
                     QIDIAAQNGLENSGSLNAAKLRVSGDSFNNTVKGKLQAHDLAVNTQTAKNSGHLLTLT
                     GKIDNRELHNAGEIAANNLTLIHSGRLSNDKKGNIRAAYLQLDTAGLHNAGNILADSG
                     TVTTKNNLHNTGKVSVARLNTEGQTLDNTHGHIEAETVNIQSQQLTNQSGHITATEQL
                     TINSRNVDNQNGKLLSANQAQLAVSDGLYNQHGEIATNRQLSIHDKNQNTLALNNADG
                     TIQSAGNVSLQAKSLANNGTLTAGNKLDIALTDDFVVERDLTAGKQLNLSIKGRLKNT
                     HTLQAGHTLKLNAGNIDNQVTGKIIGGEQTDITSEQHVDNRGLINSDGLTHIGAGQTL
                     TNTGTGKIYGNHIALDAQILLNREETTEGSTKAGAIAARKRLDIGAKEIHNQEGALLS
                     SEGIFAVGNRLDEQHHAAGMADTFVNGSAGLEVQGDALMSVRNMQNINNHFKTETYLA
                     KAEKQVRDYTVLGQNTYYQAGKDGLFDNSQGQKDQTTATFHLKNGSRIEANQWHVRDY
                     HIETYKERIIENRPAHITVGGNLTASGQNWLNKDSRIVVGGRIITDDLNQKEITNQST
                     TGKGRTDAVGTQWDSVTKKGWYSGRKRQRRTERNHTPYHDTQLFTHDFDTPVSVIQQN
                     AASPSFQPAASAIKLIDGVSTAAVNGQRIHTGNVVSLNNATVTLPNSSLYTTHPDNKG
                     WLVETDPQFADYRRWLGSDYMLQQLKLDTNHLHKRLGDGYYEQKLVNEQIHQLTGYRR
                     LDGYRSDEEQFKALMDNGLTAAKTFGLTPGIALSAEQVARLTSDIVWMENQTVTLSDG
                     STQTVLVPKVYALARKGDLNTSGGLISAEQVLLKLQNGNLTNSGTIAGRQAVLIQARN
                     INSNGNIQADQIGLKAEKSINIDGGQVQAGRLLTAQAQNINLNGTTQTSGNERNGNTA
                     IDRMAGINVVGSHTEQVDNRASDGILSLHADNDINLNAATVSNQVKGGTTQITAGNNL
                     NLDTIRTEHREAYGALDDKNHRHVRQSAEVGSSIRTQNGAMLRAGNDLKIRQGELEAD
                     EGKTVLAAGRDVNISEGRQITELDASVSGKSKGILSSTKTHDRYRFSHDEAVGSNIGG
                     GKMIVAAGQDINVRGS"
BASE COUNT         1312 a         1052 c          942 g          877 t
ORIGIN      
        1 agccatgcgg ttaagattga tgggcctgtg tggggtaaag atgtgcgtgt gtcggcaggg
       61 aaaaataatg tctcagctga tggctctatc cggtcttcac actcccctgc tacgaatata
      121 aattccgaca actcactgta tgccatcgat acaggggcat tgggcggtat gtatgccggt
      181 aagatcaccc tgatcagcac tgatcgtgat gcatccgtgc gcaatcaggg gcaatggttt
      241 gcctcagccg gcaatgtggc agtgaatgct gagggtaaac tggtcaacac aggcatgatt
      301 gcagcgacgg gagaaaatca tgcggtttca cttcatgccc gcaatgttca taatagcggt
      361 acggttgcct cacaggatga tgccaatatt cacagccaga cgctggacaa ttcaggtacg
      421 gtcttatcct caggtcaatt aactgtccgt aatttaggcc gtctgaaaaa ccataacaac
      481 ggtacgatcc aggctgcccg cttagatata tcaacaggtg gtttggataa cacaggtaat
      541 attactcaaa caggttcaca agcattggat ttggtatctg ccggcaaatt cgataacagt
      601 ggcaagattg gtgtgagcga cgttccacag accggtttga atcccaatcc atcagtcata
      661 ccacagattc cgagtactgc aacaggttca ggcagcagca ctatctcggc atctaagcct
      721 ggttcaaaca atcccgtttc acctacagca cctgcaaaaa actacgccgg aggacgcatt
      781 caaacaacag gagcatttga caatgcagga tcaatcaatg cgggtgggca aattgacatt
      841 gccgcccaaa acggtttgga aaattcgggt agtctgaatg cggctaaact acgagtatca
      901 ggcgattcat ttaacaatac ggtaaaaggc aaactccagg cacacgatct ggctgttaac
      961 actcaaactg ctaaaaacag cggtcactta ttaactctaa ccggcaagat tgacaaccgt
     1021 gaactgcata atgccggaga aattgccgcc aacaatctga cactcattca ttcgggccgc
     1081 ttgagcaatg ataaaaaagg caatattcga gctgcatatt tacagcttga taccgccggt
     1141 ttacataatg caggtaacat tcttgccgat agtggaaccg ttaccaccaa gaataatctt
     1201 cacaatacag gaaaagtttc tgttgcacga ctgaataccg aaggtcagac tctagataat
     1261 acgcacggac atatagaggc tgaaacggtt aacatccaaa gtcagcaact gactaaccaa
     1321 agcggccata ttactgctac cgaacaactg actatcaata gtcgaaatgt agacaaccaa
     1381 aacggcaaac tcctatctgc aaaccaagca caattagctg tttcagacgg cctatacaac
     1441 caacatggtg aaattgccac caaccggcag ttgtctattc acgataaaaa tcaaaacact
     1501 ttggcgttaa acaatgcgga tggcacgatt caatctgccg gtaatgtatc gctacaagcc
     1561 aaatcactcg ccaacaatgg cacattaaca gccggtaaca aactggatat tgctttgacg
     1621 gacgatttcg tcgtagagcg cgacctcact gcaggcaaac aattaaatct aagcataaaa
     1681 ggccgtctga aaaataccca taccctacaa gcaggccata cgctcaaact caatgccggc
     1741 aatatagata accaagttac aggcaaaatt attggcggag aacaaacgga catcacatcc
     1801 gaacagcatg ttgacaacag gggcttgatc aacagcgacg gtttgaccca catcggtgca
     1861 ggtcaaaccc tgaccaacac cgggacaggc aaaatctatg gcaaccatat tgccctggac
     1921 gcgcaaatac tgcttaaccg ggaagaaacg acggaaggca gtaccaaagc gggggcaata
     1981 gctgcaagga aacgtttgga tattggagcg aaagagattc ataaccaaga aggtgcccta
     2041 ctatccagcg aaggtatttt tgccgtaggt aatcgactgg atgaacaaca tcatgcggca
     2101 ggcatggccg atacctttgt taatggcagt gccggtttgg aagtacaagg tgatgcattg
     2161 atgtccgttc ggaatatgca gaatatcaat aatcacttta aaacagagac atacttagcc
     2221 aaagcagaaa agcaagttcg tgactacacc gtactggggc aaaataccta ctatcaggca
     2281 ggaaaagacg gtttattcga caactcgcaa ggacaaaaag accaaactac tgctacgttc
     2341 catttaaaaa atggttctcg tattgaggcc aaccaatggc atgtccgaga ctaccacatc
     2401 gagacttata aagaacgcat catcgaaaac cggccggcac acattactgt gggcggtaat
     2461 ttgactgcct caggtcaaaa ttggctgaac aaagacagcc ggattgtagt aggcgggcgt
     2521 attatcactg atgatttaaa ccagaaagaa attaccaatc aaagtacaac aggcaaaggt
     2581 cgcacagatg ctgtcggcac acagtgggat tcagttacaa aaaaaggatg gtacagcggt
     2641 agaaaaagac aacgccgtac tgaaagaaac catactcctt accatgatac ccaactattt
     2701 acccacgact tcgacacgcc tgtatccgtc atccaacaga atgccgcctc cccttccttt
     2761 caacccgctg catctgcaat caaactgatt gacggagtat ccacggcagc cgtcaatggt
     2821 cagcgcatcc ataccggtaa tgtggtctcg ttaaataacg ctactgttac tctgcctaac
     2881 agcagcctct ataccaccca tcctgacaat aaaggctggt tggttgaaac cgatcctcaa
     2941 tttgcagact accgccgctg gttgggcagc gactacatgt tgcaacaact gaaattggac
     3001 accaatcatc tacacaaacg gcttggcgac ggctactacg aacaaaaact tgttaatgaa
     3061 caaatccatc agttaacagg ctaccgccga ctcgacggct acaggagtga tgaagaacaa
     3121 ttcaaagctc tgatggacaa cggccttact gctgccaaaa cattcggtct caccccaggt
     3181 atcgccttga gtgcagagca agttgcccgc ttaacttcag atatcgtttg gatggaaaat
     3241 caaaccgtca ccctgtctga cggttcgact caaaccgtac tggttcctaa agtctatgcc
     3301 ctggcgcgca aaggtgatct caatacctcc ggtggcctga ttagtgccga acaagtctta
     3361 cttaaactgc aaaacggcaa cctgactaac agcggtacca ttgcggggcg acaggccgta
     3421 ctcatccagg cacggaatat taacagcaac ggtaacattc aagccgacca aatcggctta
     3481 aaagctgaaa aaagtatcaa tatcgacggc gggcaggtac aagcaggcag actgctgact
     3541 gcccaagcgc aaaatatcaa ccttaacggt acaacccaaa cttccggtaa tgaacgtaac
     3601 ggcaataccg ccatcgatcg tatggccggc attaacgtgg tcggaagcca tactgaacaa
     3661 gtagataaca gagcttcaga cggcatccta tccctgcatg ccgacaacga tatcaacctt
     3721 aatgcagcca ccgtctctaa ccaagttaaa ggcggcacca cccaaattac cgctggtaat
     3781 aatctcaacc tcgacaccat ccgtaccgaa catcgcgaag cctatggtgc attagatgat
     3841 aaaaatcatc gtcatgtccg ccaaagtgcc gaagtcggca gcagtatccg cacgcaaaac
     3901 ggcgcaatgc ttagagccgg caacgactta aaaatccgtc aaggtgaact ggaggccgac
     3961 gagggtaaaa ccgtccttgc cgcgggacgt gatgtcaata ttagcgaagg acgccaaata
     4021 accgaattgg acgcctctgt aagcgggaaa agcaaaggca tcctttccag taccaaaaca
     4081 cacgaccgct accgcttcag tcatgatgaa gcagtcggca gcaacatcgg cggcggcaaa
     4141 atgattgttg cagccgggca ggatatcaat gtacgcggca gca
//
LOCUS       DAXHRI010000161         4122 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXHRI010000161 DAXHRI010000000
VERSION     DAXHRI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.161"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..302)
                     /locus_tag="ACFM75_001839"
     CDS             complement(<1..302)
                     /locus_tag="ACFM75_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6256337.1"
                     /translation="MEIINDAELIRSMQRQQHIDAELLTDANVRFEQPLEKNNYVLSE
                     DETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTGMCLGSNNLSRLQKAAQQIL"
     gene            330..1232
                     /locus_tag="ACFM75_001840"
     CDS             330..1232
                     /locus_tag="ACFM75_001840"
                     /inference="COORDINATES: protein motif:HMM:NF038290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFC6256338.1"
                     /translation="MKDACNNLFERQFSFIEKTPKGEKVVRTRWVSQVAYIEQQATVE
                     LVFAPNVAPLITMLEKNFTSYELDQVSSLSSKYAVRLYEIIISWRAAGKTPMFSTMEL
                     RERLGVMPDEYQKMELFKRKVLDFAVKQINDKTDISITYEQHKEGRKIVGFTFSILHK
                     IGSKDIPLENQSELFAGMTDLEAGTIRVREEAYIASLIAKGQNVTKAHRLNILKKAVD
                     ERWGFEDAAKDNGTKNPENKNAKVVLNEWEKIPNGTRFKDKDGTIWVKDSGLLRTEET
                     NRWIADSQIARLFPMLTVMVEEGI"
     gene            1329..1415
                     /locus_tag="ACFM75_001841"
                     /pseudo
     CDS             1329..1415
                     /locus_tag="ACFM75_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(1414..1512)
                     /locus_tag="ACFM75_001842"
                     /pseudo
     CDS             complement(1414..1512)
                     /locus_tag="ACFM75_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(1727..2866)
                     /locus_tag="ACFM75_001843"
                     /pseudo
     CDS             complement(1727..2866)
                     /locus_tag="ACFM75_001843"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            2892..3326
                     /gene="tnpA"
                     /locus_tag="ACFM75_001844"
     CDS             2892..3326
                     /gene="tnpA"
                     /locus_tag="ACFM75_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFC6256339.1"
                     /translation="MILHLVYGKETDLRRGRHVVFNLHVHLVFVAKYRRKVFTKEILD
                     DMRQIFESVCTDFEAQLVEFDGENDHVLLLVNYPPKVSISKLVNSLKGVSSRMIRQKN
                     YPGIREKLWGGALWSPSYFAGSCDGASISIIRQYIEQQNTPD"
     gene            3436..>4122
                     /locus_tag="ACFM75_001845"
     CDS             3436..>4122
                     /locus_tag="ACFM75_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFC6256340.1"
                     /translation="MNRTLYKVVFNKHRNCMIAVAENAKREGKNTADTQAVGILPNDI
                     AGFAGFIHSISVISFSLSLLLGSALILTSSSANAQGIVADKSAPAQQQPTILQTGNGI
                     PQVNIQTPTSAGVSVNQYTQFDVGNRGAILNNSRSNTQTQLGGWIQGNLWLARGEARV
                     VVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFINASRATLTTGQPQYQA
                     GALTGFQVRSG"
BASE COUNT         1119 a          874 c          914 g         1215 t
ORIGIN      
        1 atcagtatct gttgcgcagc tttttgtagc ctgctcaaat tattggaacc taaacacatc
       61 ccagttttaa aagctgtttc tttcatgagc acagaaggaa gaaaagaaaa tttgcgcacc
      121 gtcttatcat ctaaactaat gtaatttacc cgagtacacg gtgtttcatc ttcactcagg
      181 acataattgt tcttctccaa tggttgctcg aaacggacat ttgcatcagt taacaattca
      241 gcatctatgt gctgctgacg ctgcatggaa cggataagtt ctgcatcgtt aataatttcc
      301 ataccaaccg taatacggcg tataaggctt tgaaagatgc ctgtaacaac ctttttgaac
      361 gccaattcag ttttatcgaa aaaacaccaa aaggggaaaa ggtagtacgg acaaggtggg
      421 tatctcaagt cgcctatatt gaacaacagg caacggtaga gttagttttt gcaccaaatg
      481 ttgccccttt gattacgatg ctagaaaaaa acttcacaag ctacgagctt gatcaggtct
      541 catcgttgag cagtaaatac gcggtgcggc tctacgaaat tattatttca tggcgtgcag
      601 ccggtaagac accgatgttc agtacaatgg agttgcgcga acgtttgggt gtgatgcctg
      661 acgagtatca aaaaatggag ctattcaaac gtaaggtttt ggatttcgcc gtcaagcaga
      721 tcaatgataa aacggatatt tccattacct acgagcagca taaagaagga cgaaaaattg
      781 taggttttac attctcaatc ctacataaaa tagggtcgaa agacatccct cttgaaaatc
      841 aatcggaact ttttgccgga atgactgatt tggaagccgg aacgatacgg gtcagggagg
      901 aagcatatat cgcttcgctc attgcaaaag gtcagaacgt gactaaagcc cataggctga
      961 atattctgaa aaaagccgta gatgaacgtt ggggatttga agatgcggct aaagataatg
     1021 ggaccaaaaa tcctgaaaac aaaaatgcaa aagtcgtttt aaacgaatgg gaaaagattc
     1081 ctaatggcac acgttttaag gataaggatg gaacaatttg ggttaaagat tcaggtctgc
     1141 ttaggacaga agagacaaac aggtggattg ccgattctca gattgccaga ttatttccta
     1201 tgttaactgt gatggttgag gaaggtattt aacccggcag cccgcgtatc ctacctgttt
     1261 tgcggtacgg aaaccaattg aacctgcttt acgctacaat agaagattgc aatttcggcg
     1321 gatgtaccat gaacgattac acagccatgc cgtctgaaga cggaggaatc agctcattat
     1381 cgcttccgcc gcactcaatg gaggcggagc agtcaaaata tcggcagcgg aacgggtggt
     1441 agcttggagt acgaaaaatt caagcagttt cttttgaact ctattgttta atttacaatt
     1501 ggttatcttt attttcgtag cctggcataa ctgctaatct acgtcagccc ctaaaaatta
     1561 atctaattta cgattttgac atcctccccc gcctaaaggg cgggggattc ctgctgcggt
     1621 cggcggtgtt ctgccaaacc gcttcggtgg gttcgtgctg ctgacaacct taccgcattg
     1681 ttcacttcac acgcgctacg ggcatgtcct gccctgattt gactgcttat tgcgccgcca
     1741 agatttcatg accgcgtcgt aatatattta tcgccccagc cacatccgca ttttctgtat
     1801 agccgcattc tacgcattcg aaatcagcct gcgtttggcg gttttccttt gcggtataac
     1861 cgcaacaagg acaggttcga ctggtatttt gcggcggtac ggcaaccaga tgtccgccat
     1921 tccacaataa tttgtaatcc aactgctgtc tgaactcaaa ccaagactga tccagtatca
     1981 tccggttcaa acctgatttc tgtttcacat tcttgccgtg ctgttcaaca tctcctttgg
     2041 cggatttgga catgtttgtt acctgcaaat cttcaacata cacaatcgcg tggctttggc
     2101 tgatttggct gctgatttgg tgcaagtagt ttttacggat attgctgatt ttgtggtgca
     2161 atttggcgat tttcgccttt agtttctgcc agttttagct aaatttaacc ttgtttttaa
     2221 gtcggcgttg tagttttgcc agtttgcctt tcaggttctt gaatgcgtta atcggctcaa
     2281 aatattcgct gcttgaaagt gtcgcaaaac gaacaatacc tatatcgata ccgatttctc
     2341 cgcctttggg ttgcggtgtt ttttgttcaa attctgtttg aacggagata taccacttac
     2401 cgcattttcg gctgacggtt acgttcttca tttcgccgac aatggcacgg ctgttgcgat
     2461 aacgtatcca accgattttg ggcagataga tgcggttatt ttgttgttcc agtttaaagc
     2521 cttgcggaaa tctgaaactg tctttttcgc ccttgcgttt gaattttgga aagtccgaac
     2581 gtttggcgaa gaagtttttg aatgagcttt ctaaatcttc gagcgattgc tgcaaaactt
     2641 ggctgtggca gtcttttagc caagttaatt cacgtttcca ttcaggcaac aaattcgcga
     2701 ttttgttgta actgaatttg aatgatttat ctgcttcgta ctgttcattt tgatacgcca
     2761 aagcgcgatt gaatacaaaa cgggaacaac cgcaaaactg cttaagtctg cggatttgtg
     2821 cgccgtttgg catcaattca aatttgaacg ctttgagtat ttgcatggtt tcgtagataa
     2881 tggaattttt gattattcta cacttggtct atggaaaaga aactgattta agacgtggta
     2941 gacacgttgt ttttaactta catgttcatt tagtctttgt cgcaaaatac cgccgaaaag
     3001 tgttcacaaa agaaatttta gacgatatgc gacaaatttt tgaaagcgtt tgcaccgatt
     3061 ttgaggcaca actagtggaa tttgacggtg aaaatgacca tgttcttttg cttgtaaact
     3121 atccacccaa agtatctatt tcaaaactcg tgaacagctt gaaaggtgta tctagtcgta
     3181 tgattaggca aaaaaattat cccggtattc gtgagaaact ttggggtggt gcgttgtggt
     3241 cgccgtctta ttttgcaggt agttgtgatg gtgcatctat ttccattatt cgacaatata
     3301 ttgagcagca aaatacgcct gactgaaatt caacgttttt aggactgctt tgcagtccgt
     3361 gcctgatatc tccgccgtga acggcgaagt tttacggcgc attcggataa aaatattatt
     3421 aagaaagccc ttactatgaa ccgcaccctg tacaaagttg tatttaacaa acatcgaaac
     3481 tgcatgatag ccgttgctga aaatgccaaa cgcgagggca aaaacacagc cgacacccaa
     3541 gctgtaggta ttttgccaaa tgatattgcg ggctttgcgg gttttatcca ttctatctct
     3601 gttatctcat tctccctttc attactgctc ggttctgccc ttatcctgac ttcttcttct
     3661 gctaatgccc aaggtatcgt tgccgacaaa tccgcacctg cacagcaaca gcctaccatc
     3721 ctgcaaacag gtaacggcat accgcaagtc aatattcaaa cccctacttc ggcaggggtt
     3781 tctgttaatc aatatacaca gtttgatgtg ggtaatcgcg gggcgatttt aaacaacagc
     3841 cgcagcaaca cccaaacaca gctaggcggt tggattcaag gcaatctttg gttggcaagg
     3901 ggcgaagcac gtgtggttgt aaaccaaatc aacagcagcc attcttcaca aatgaatggc
     3961 tatattgaag tgggcggacg acgtgcagaa gtcgttattg ccaatccggc agggattgca
     4021 gtcaatggtg gtggttttat caatgcttcc cgtgccactt tgacgacagg ccaaccgcaa
     4081 tatcaagcag gcgcattaac aggctttcag gttcgttcgg gt
//
LOCUS       DAXHRI010000162         4105 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXHRI010000162 DAXHRI010000000
VERSION     DAXHRI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.162"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            30..557
                     /locus_tag="ACFM75_001846"
     CDS             30..557
                     /locus_tag="ACFM75_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC6256341.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            645..1700
                     /gene="plsX"
                     /locus_tag="ACFM75_001847"
     CDS             645..1700
                     /gene="plsX"
                     /locus_tag="ACFM75_001847"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC6256342.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            1836..2087
                     /locus_tag="ACFM75_001848"
     CDS             1836..2087
                     /locus_tag="ACFM75_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6256343.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            2114..2529
                     /locus_tag="ACFM75_001849"
                     /pseudo
     CDS             2114..2529
                     /locus_tag="ACFM75_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2549..2752
                     /locus_tag="ACFM75_001850"
     CDS             2549..2752
                     /locus_tag="ACFM75_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256344.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            2779..3741
                     /locus_tag="ACFM75_001851"
     CDS             2779..3741
                     /locus_tag="ACFM75_001851"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC6256345.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            3788..>4105
                     /locus_tag="ACFM75_001852"
     CDS             3788..>4105
                     /locus_tag="ACFM75_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC6256346.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         1101 a         1199 c          968 g          837 t
ORIGIN      
        1 caaaagcccc aaccaccaag gaaattccga tgaaacagaa aatctggtac acctacgatg
       61 acatccaccg cgtcatcaaa gcattggcag aaaaaatccg gaacgccgac atcaaatacg
      121 atgccatgat tgccatcggc ggcggcggct ttattccggc acgtatgctg cgctgttttc
      181 tggaaattcc gatttatgcc gtaaccaccg cctattacga cagcgacaac gaaggacagg
      241 ttacagaaga agtcaaaaaa gtccaatggc tcgaccccgt tcccgaagcc ctgcggggca
      301 aaaacgtact cgtcgtcgat gaagtggacg acagccgcgt aaccatggaa ttttgcctga
      361 cagagctgct caaagaagac ttcggtacga tcggcgtcgc cgtactgcac gaaaaaatca
      421 aagccaaagc aggcaaaatc cccgaaggca ttccctattt cagcggcatc accgtagaag
      481 actggtggat caactatccg tgggacgcac tcgacatcga cgaacacaac cgccttgccg
      541 aggccggccg aggctgaccc tttcagacgg catattttcc gaaccgatgc cgtctgaagc
      601 ccgcacgacc cctgccgcag accgaaaacc taccggagaa ccctatgatt acacttgccg
      661 tagatgccat gggcggcgac caaggacttg ccgttaccgt acccggcgca accgcattcc
      721 tccaagcaca ccccgatgtc cgcctgatta tgaccggcga cgaaacgcaa ctgcgccaag
      781 ccctgaccgc ggcaggcgca ccgatggaac gcatcgacat ctgccatacc acccaagttg
      841 tcggcatgga tgaatcccct cagtccgccc taaagaacaa aaaagactcc tccatgcgcg
      901 ttgccatcaa ccaagttaaa gaaggcaaag cccaagccgc cgtatccgca ggcaacacgg
      961 gcgcactcat ggcaaccgca cgtttcgtcc tcaaaaccat tcccggcatc gaacgccccg
     1021 ccatcgccaa attccttcct tccgacaccg accacgttac cctcgccctc gacctcggcg
     1081 caaacgtcga ctgcacgccc gaacagctcg cccaatttgc cgttatcggc agcgaactcg
     1141 tccacgcact ccatcctcaa aaaggacagc cgcgtgtcgg gctggtcaac gtcggcacgg
     1201 aagacatcaa aggtacggac accgtcaaac aaacctacaa actgctgcaa aacagcaaac
     1261 tcaactttat cggcaacatc gaaagcaacg gcatcctgta cggcgaagca gatgtcgtcg
     1321 tcgccgacgg ctttgtcggc aacgtcatgc tcaaaaccat cgaaggcgcg gtcaaattca
     1381 tgagcggagc catccgccgc gaattccaaa gcaacctgtt caacaaactt gccgccgttg
     1441 ccgccctacc cgccctcaaa gggctgaaaa acaaactcga tccgcgcaaa ttcaacgggg
     1501 ccatcctgct cgggctgcgc ggcatcgtca tcaaaagcca cggcggcaca gacgaaaccg
     1561 gtttccgcta cgcactcgaa gaagcctacc acgaagccaa gtccgccggc ctttccaaaa
     1621 tcgaacaagg cgttgccgaa caactcgccg cactcgaaac tgccaaagcc gtccaaaacg
     1681 aaaatgccga cggtctgtaa cacacacgat gccgtctgaa cgcccccgcc cctttcagac
     1741 ggcatccgcc cgcaccaaac ctgcgggcgc ggacggcgat gcgcctgtcc ggcacttccc
     1801 aaatacttcc ctgtaaaata aggagtattt gaaaaatgaa gacattagaa aaacggatga
     1861 aagctctaga caaacagatg atgaagttcg gaaaatccct tgaaggcagg cttgatgccc
     1921 gtctgattga atccgcattg gattatattc attattcgga acgttttttg gcttttgaaa
     1981 tcctgtgtac ttatatcgaa gatttcgatg tccggctgac ggaacaagaa tcccgggaaa
     2041 tttcttttat caacaaggaa tttgagatag aaagcacgtc cgattaacca ataaagccaa
     2101 tgggttgata aacatgaaaa catcgacggt cgtttttggc ggatttttta tggcagacaa
     2161 cggagagcga atccaaatcc ccgttttgga aaatcctgac attagggaaa tcaatcactt
     2221 ttttccgtat caaattttga gaaaaaagcc ggcgtccttg ttttcagaat catccccgag
     2281 ccggaatttg gcgataccga attaactatc tattttgaaa aaggatatta tttgccaatg
     2341 attcaaacaa ttttagaaga cggggatatt gaagtcaaaa accttaaaac agaaaattac
     2401 agcgggaagg caagggaaat cttgggagat tcctacccta ttgagcatat ctctaaaaat
     2461 atttctacaa ttcaaaatat aatttctgaa tttatgatac aaaaacaaac accggcacca
     2521 ataatttgat ggaaaaccaa caatcaaaat gccgtctgaa gcatgtttgg ttttcagacg
     2581 gcatttcctt cgcttaaaac agcgtatcgg caaccccgcc ctgcctgtcc acggcaatct
     2641 gcatctgaaa accatctgta tcccaaacca cacccccatc cctgtttccg tcatttgcac
     2701 cctgcccgta ttgggcaatc atcggttttt cgcttacaat agccgaatct gaaccaactc
     2761 tctaaaaagg ccgttcccat gcagtatgcc aaaatttccg gcacgggcag ctatcttcct
     2821 gccaaccgcg tcagcaatga cgaccttgcc caaaaggtag atacctctga cgagtggatt
     2881 accgcgcgca cgggcatcaa attccgccat attgcagccg aaaacgaaaa aaccagtgac
     2941 cttgccgccg aagcggcgcg ccgcgcgctg gatgcagccg gattagacag cggcgaaatc
     3001 gatttgatta tcgtggcaac ggcaacgccg gatatgcagt ttccttctac cgccaccatc
     3061 gtgcagcaaa aattgggcat caccaacggc tgccccgcgt ttgacgtaca ggcggtgtgc
     3121 gccggcttta tgtacgcgct gaccacggca aacgcctaca ttaaaagtgg tatggcgaaa
     3181 aacgcgctgg tcatcggcgc ggaaaccttc agccgcattg tagactggaa cgaccgcaca
     3241 acctgcgtat tgttcggcga cggtgcgggc gcggtggttt taagcgcggc ggacaaaccg
     3301 ggcatcatcc acagcaaact caaggccgac ggcaattatc tgaaactctt aaacgtcccc
     3361 gggcaaatcg cctgcggcaa agtttccggt tcgccgtaca tttcgatgga cggtcccggc
     3421 gtgttcaagt ttgccgtcaa aatgctgtcc aaaatcgccg atgacgttat cgaagaagcc
     3481 ggttacaccg ccgctcaaat cgactggatc gtgccgcatc aggcaaaccg ccgcattatc
     3541 gaatcgaccg cgaaacattt aggtttgagt atggacaaag tcgtcctgac cgtccaagac
     3601 cacggcaaca catccgccgc ctcgattccg ctggctttgg atacgggcat ccgcagcgga
     3661 caaatcaaac gcggtcaaaa cctgctgctc gaaggcatcg gcggcggttt cgcgtggggc
     3721 gcggtgctgt tgcaatattg aacccgatgc cgtctgaaac aggctttcag acggcatttc
     3781 ccatatcatg aagcggcagg ctttcttcaa actgatggcg tgtgcggcat ttctgtctgc
     3841 cgtttcgctg cgcctccccg tattgggcgc gtgttacgca atattgtccc tctatgcgtt
     3901 tgcactttac ggcatcgaca aacggcgtgc cgtgcgggga aaacgccgca ttcccgaaca
     3961 ccgcctgctg ctgcctgcct tgttcggcgg ttgggcgggc gcatacttgg gcagcaggat
     4021 attcaggcat aaaacggcga aaaagcgttt tgttgtgctg ttccgtctga ctgtttcggg
     4081 caatgtcctg gcgaccctca tcctg
//
LOCUS       DAXHRI010000163         4037 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXHRI010000163 DAXHRI010000000
VERSION     DAXHRI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.163"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..275)
                     /locus_tag="ACFM75_001853"
     CDS             complement(<1..275)
                     /locus_tag="ACFM75_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256347.1"
                     /translation="MSNNLTLADVPDFSDWRLAPLWTLEQAALLWAGINPAFSTFEDI
                     WKEHPRKQAWAKIALQAFLGGIVLKTLPVHELYLLDAGGWAYCDNQKT"
     gene            complement(279..1376)
                     /locus_tag="ACFM75_001854"
     CDS             complement(279..1376)
                     /locus_tag="ACFM75_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6256348.1"
                     /translation="MRKNAMMYDPLYFPRSELAEIIAKSLYGGITHAFTLFAPRRMGK
                     TQFLLKDIKPTAEAAGFNVFYFSFMDSTAPTVDFQAALLRFAESIKTGGKVQNLIGQI
                     SKIEIMGSSIERRTERQQAELRLSDIIADIAGGSRPSLLLLDEIQELARQPDTGGLIR
                     SLRTGLDIHQQQVKTIFTGSSTNGLRAMFNDIKAPFFHFAHALDFPTLGREFTDFLAG
                     VYQERTGRGLDGGELYRAFERLNKTPLYMRAMLQDMIINPDLSIEQAESIRLEEMHKH
                     ADTAAQWRHLSLIEQLILSELANGQTAIYSVQTRQALARVIGVDDIGTSTVQNNIRKL
                     ERKDLITRDGANRLQINSPILKTWILENVER"
     gene            complement(1606..2022)
                     /locus_tag="ACFM75_001855"
     CDS             complement(1606..2022)
                     /locus_tag="ACFM75_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC6256349.1"
                     /translation="MYLLDTNVISETRRANRADGNVRAWLNTIDPAEMYTSAIVMMEL
                     ERGVLGMERKDGQQGGILRRWLEEVVKPTFKDRILPINETTAEICARLHIPDKSPEND
                     AWIAATAKQHGLTLATRNIKDFEHSGVKLINPFETV"
     gene            complement(2025..2300)
                     /locus_tag="ACFM75_001856"
     CDS             complement(2025..2300)
                     /locus_tag="ACFM75_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFC6256350.1"
                     /translation="MLNTVSSREFNQYPSQTQKKAAKSPVFITTRGKPSHVLISYAEY
                     RKLTGKPQSALEALTANAELAAKLESIDFEIPPRSTAQRPPVDFGED"
     gene            complement(2863..3954)
                     /locus_tag="ACFM75_001857"
     CDS             complement(2863..3954)
                     /locus_tag="ACFM75_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC6256351.1"
                     /translation="MYLYVNTGGGKLWRFDFSHNGKRKTLSIGKYPTVSLVEARQTAE
                     NARRLLAAGQDPGEAKQQEKRERQAAVSNTFRSIAAEWHKHRETGLTGNHAARVWDSL
                     KKDVFPMLGGLPVSEIKVSQVKEVIEAVAARGALETAGRILQRIKAVFTYAIQTERAE
                     NNPAVPLVGLIKAPKQKHQPALPQSELTEFYRRLMLEQAERQTKIAMQLIMLTFVRNG
                     ELRAAEWVEFDLNGAEWSIPAAKMKMKAPHIVPLADWTLELLAELKEITGHSRYLFPS
                     VKDPDKHMSENILSYLMGRMGYKGIATPHGFRSLATDVLNENGFDSDVIERQLAHVEQ
                     NKVRAAYHRTEYIPQRKELMQWYSDCLRE"
BASE COUNT          826 a         1052 c          931 g         1228 t
ORIGIN      
        1 gttttttggt tgtcgcaata agcccaaccg cctgcatcaa gcaaatacaa ctcatgcacc
       61 ggcagtgttt ttaaaacaat accgcccaaa aaagcctgta atgcaatttt tgcccacgcc
      121 tgtttgcgcg ggtgttcctt ccatatatcc tcaaaagtgg aaaacgccgg gttaattccc
      181 gcccacaata aagcggcttg ttccagcgtc cacaacgggg ctaaacgcca atcagaaaaa
      241 tcaggaacat cagcaagggt taaattgtta ctcattccct accgctccac attctctaaa
      301 atccaagtct ttaaaatcgg gctgtttatc tgcaacctgt tcgcgccgtc tctggttatc
      361 aggtctttac gctctaattt gcggatattg ttttgcaccg tgcttgtgcc tatatcgtcc
      421 acaccgatta cacgggctag ggcttggcgc gtttgcacgg aatatatcgc ggtctgcccg
      481 tttgccaact ccgataaaat caactgttct atcaggctca aatgccgcca ttgcgcggct
      541 gtgtctgcgt gtttgtgcat ttcctccagc cttatgcttt cagcctgctc tatgcttaaa
      601 tcggggttga ttatcatgtc ctgcagcatg gcgcgcatat aaaggggcgt tttgttcaac
      661 cgttcaaatg cgcggtacag ttcgccgccg tctaaccccc tgcccgtcct ctcctgatac
      721 acaccagcaa gaaaatcggt aaattcccgt ccaagcgtgg gaaagtctaa ggcgtgggcg
      781 aaatggaaaa acggggcttt gatgtcgttg aacatggcgc gtaaaccgtt tgtgctgctg
      841 cccgtgaata tggtttttac ctgctgctgg tgtatgtcca agcctgtacg caaactgcgt
      901 atcaagccgc ccgtatcagg ttgccgtgcc agttcctgta tttcgtccaa cagcaacagg
      961 ctggggcggc tgccccctgc aatgtcggcg atgatgtcgg acagccgcaa ttccgcctgc
     1021 tgccgctccg tccgtctctc tatgctgctg cccatgattt ctattttgct gatttgccca
     1081 atcaggtttt gcacctttcc gcccgtcttg atactttcgg caaagcgcaa taatgccgcc
     1141 tgaaagtcaa cagtgggcgc ggtgctgtcc atgaatgaga aataaaagac attgaagccc
     1201 gccgcctctg ccgtgggctt gatgtctttc aaaagaaact gtgttttgcc cattcttcgc
     1261 ggcgcaaaca gcgtaaaggc gtgtgttatg cctccataca ggcttttggc gatgatttcc
     1321 gccaactctg aacgggggaa atataagggg tcatacatca ttgcattctt tcgcattgtt
     1381 actcattagc ccgtattata caccgtaaag ctaattatta atacggtcaa taattttgta
     1441 taacgcgcgg cgcaccgtca gcgcgtttta gctggtgtag gtgtgaaaaa tccccgtacc
     1501 tgtcggttat acggggatgt tttcggcggc tttcgcgcct gtttgcgccg cgcctttcat
     1561 attggttatg ccgtccttgt tatccattcg ttgaggctga tatttttaga cggtctcaaa
     1621 cgggtttatc aatttcacgc cgctatgctc aaagtctttg atatttcgcg ttgccaaagt
     1681 taaaccgtgc tgcttggctg ttgccgctat ccaggcgtcg ttttcggggc ttttgtcggg
     1741 tatgtgcaaa cgggcgcata tttcggcggt ggtttcgttt atgggcaata tgcggtcttt
     1801 gaatgtgggc tttacaactt cttccagcca gcggcgcaat attccgccct gctgtccgtc
     1861 tttgcgctcc atacccaaaa cgccccgttc aagttccatc atgacaattg cgctggtgta
     1921 catttcggcc gggtcaatgg tgtttagcca tgctctaacg ttgccgtctg cccgatttgc
     1981 ccggcgtgtt tcgctgataa cgttggtatc caacagatac attttcaatc ctccccaaaa
     2041 tcaacgggcg gtctttgcgc ggtgcttcgc ggcggtattt caaaatcgat gctttccagt
     2101 ttggcggcaa gctcggcgtt tgccgtcagt gcctccagtg cgctttgcgg cttgcctgtc
     2161 agtttgcggt attccgcata actgattaat acatggctcg gcttgccgcg tgtggtaata
     2221 aatacggggg actttgcggc tttcttttgt gtttggctgg ggtattggtt aaattccctg
     2281 ctgctgacag tgtttaacat tacgccctcc ttgtgtcttg tttgtagtta tgtagcctct
     2341 ttttacgcga ttgtgcctgt cgtgtcaatg tgcattgccc ttgtgcctgt gttgccgtct
     2401 gaaacaccag cggccaacct gattattttt taatcaatcg cttccaatgg ctcaaatttg
     2461 cgtttttagc gtattttgcc gtctgcctat gtcttggata tatccaagcg gtttaaccgc
     2521 cattcctgct caaatttggc gcatttccgt gcgttttagc ttttcattcc gctacgcccc
     2581 cactgccaag acaggaaaaa agccgaaaat gcggggcagt tgttaccact tgttacgatt
     2641 tgctaccgca cttgttaccg attttttgta tttttattat tttatttcat atacttattc
     2701 atctaacttt ttctcggtaa caacgtaaca actaagggta tggtattcct atatagggat
     2761 tttcagaaag ccaaaaaaaa ccgtcctgta tcttggacgg tctcccctca ttccctacag
     2821 cttggcggta tctcctgcaa gctctttttc cgcttggcgg tatcactccc gcaaacaatc
     2881 actataccat tgcataagct ctttacgctg tgggatatat tccgttctgt ggtatgccgc
     2941 cctgactttg ttttgttcca catgggcaag ctgtcgctct atcacatcgc tgtcaaagcc
     3001 gttctcattc aaaacatctg tggctaggct tctaaagccg tgcggtgttg ctatgccctt
     3061 gtagcccatt ctgcccatta agtaagacag gatgttttca ctcatgtgct tatcagggtc
     3121 ttttaccgat gggaataaat agcggctatg ccctgttatt tcctttagtt ctgccaaaag
     3181 ctcaagcgtc caatcagcaa gggggacgat atgcggggct ttcatcttca tcttggcggc
     3241 aggtatgctc cattctgccc cgtttaaatc aaattccacc cattccgccg ccctaagctc
     3301 tccattcctg acaaaagtca gcatgattaa ctgcatggct atttttgttt gccgttcggc
     3361 ttgttccaac atcaaacggc ggtaaaactc ggtcagctcg gattgcggta atgcgggctg
     3421 gtgtttctgc tttggtgctt taattaaacc taccaaaggg acggcgggat tattctctgc
     3481 ccgctccgtc tgtatggcat aagtgaaaac agccttgata cgctgcaata tccgccctgc
     3541 cgtttcaagt gctcctcttg ccgctaccgc ttcaatcact tcttttactt ggctgacttt
     3601 gatttcactg acaggcaaac caccaagcat ggggaaaacg tcctttttta aactatccca
     3661 tacacgggcg gcatgattgc ccgtcaatcc tgtttcacgg tgtttgtgcc attcggcggc
     3721 aatgctccga aatgtgttac tgacagcggc ttgccgttca cgcttttcct gctgcttggc
     3781 ttcgccgggg tcttgccccg ctgcaagcaa gcggcgggca ttttcagcgg tttggcgggc
     3841 ttccaccagt gatacggtcg gatatttgcc gattgaaagc gttttacgtt tgccgttatg
     3901 cgaaaagtca aaccgccata acttcccgcc gcccgtattg acatacagat acaaaccttt
     3961 gccgtcattc agcttatacg gttttttcgg cgggcttggc gtttttgatt tgtcgatcgt
     4021 tcagcttcat ttttact
//
LOCUS       DAXHRI010000164         3962 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXHRI010000164 DAXHRI010000000
VERSION     DAXHRI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.164"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..591)
                     /locus_tag="ACFM75_001858"
     CDS             complement(<1..591)
                     /locus_tag="ACFM75_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6256352.1"
                     /translation="MGRHFGRRRFLTAAAVAAAGAAVSFLPNPFAAGGEKRNGDEKRD
                     ENVFFWKGVALGSGAELRLFGVDNRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPSADFLELLSLCRDIHALTDGAFDPTVQALWKLYADYFAARPDAETPPPQN
                     SVKEALKRVGFGKVSFDDREIRFAEKGMGLSLNGIAQ"
     gene            complement(600..1394)
                     /locus_tag="ACFM75_001859"
     CDS             complement(600..1394)
                     /locus_tag="ACFM75_001859"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC6256353.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1817..3151
                     /gene="gdhA"
                     /locus_tag="ACFM75_001860"
     CDS             1817..3151
                     /gene="gdhA"
                     /locus_tag="ACFM75_001860"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC6256354.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3293..3667
                     /locus_tag="ACFM75_001861"
     CDS             3293..3667
                     /locus_tag="ACFM75_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256355.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3711..>3962
                     /locus_tag="ACFM75_001862"
     CDS             3711..>3962
                     /locus_tag="ACFM75_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HFC6256356.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYW
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQ"
BASE COUNT         1069 a         1043 c          942 g          908 t
ORIGIN      
        1 ttgcgcgatg ccgttcaagg acaaacccat acccttttcg gcaaaacgga tttcacggtc
       61 gtcaaacgag actttgccga agccgacccg tttcagggct tccttcacgc tgttttgagg
      121 aggcggcgtt tccgcatcgg gacgggcggc aaaataatcg gcatacagtt tccacaacgc
      181 ctgcactgtc ggatcgaacg cgccgtccgt cagcgcgtga atatcgcggc acaggctcaa
      241 cagttccaaa aaatccgccg acggcgaagt cagataaccg tccctgttca ggcggctgat
      301 caggctgtct tcacggtaaa ggctgaacat tttttccaaa cgcgccactt ccgccaaaac
      361 cttgttgacc aaatccgccg cctgtctgtt atccacgccg aacaggcgca gctccgcgcc
      421 ggaacccagc gcgacacctt tccagaaaaa cacattttca tcgcgttttt catccccgtt
      481 gcgtttttcg ccgccggcgg caaaaggatt cggcaaaaaa gaaaccgccg cgcccgccgc
      541 cgcaacggcg gcagccgtca aaaaacgcct gcgcccgaaa tgcctgccca taccgcctcc
      601 taaaccgaca ctgccgcctt gatgtgcgga tgggggtcgt agccttccaa ctcgaaatct
      661 tcaaacttga aggaaaacaa atctttgact tcaggattga ttttcatcac tggcaaggcg
      721 cgcggttcgc gttccaactg caatgcggcc tgctcgaaat ggttgcggta caaatgcgcg
      781 tcgccaaacg tatggacaaa ctcgcccgcc tccagcccgc acacttgcgc catcatcatc
      841 gtcaacagcg cgtagctggc aatattaaac ggcacaccaa ggaaaatatc tgcactacgc
      901 tggtaaagct ggcaggacag tttgccgtcg gcaacgtaaa actgaaacag cgcgtggcag
      961 ggcggcaagg ccatttcatc gaccaaagcc ggattccacg ccgatacaat caggcggcgc
     1021 gagtcgggat tcttcttgat ttgttccagc acattggcga tttggtcgat atgcctgccg
     1081 tcgggcgcgg gccagttacg ccactggtag ccgtaaaccg ggcccaagtc gccgttctcg
     1141 tccgcccact cgtcccaaat ggaaacattg ttgtccttca ggtatttgat attggtatcg
     1201 cctttgagaa accaaagcag ctcgtggata atcgagcgca aatgcagctt tttggtcgtc
     1261 agcagcggaa aacctttgcc caagtcaaaa cgcatctgat aaccgaatac ggagcgcgtg
     1321 cccgtaccgg tgcggtctga tttgtccgta ccgttgtcga ggacgtggcg catcaagtcc
     1381 aaataggcct tcatcatcat ctttcatcaa attaaacggc gcatattgta acatttccgg
     1441 ccaacgcccg agtccggaca aacgcacacg ccgccccgcc ggacgataaa aacgcagata
     1501 cgggcaggca ggattgttgg caatttcagt ccttttccac agtaaaaccc ggtgggaaaa
     1561 caaaattacc ttgattggaa tcaaaaaatc tagtttaatt acttagaata aaatttcaat
     1621 aatattttta tttacgaaat taatttatga ttattgatta attatcggca acaaacatca
     1681 aacatcgaaa acatggaaaa aataatgtca acaatttttg ccaaatcggg cttggcatca
     1741 gaaaaaaata ggtttatatt cccacctaca aatttgtttt cccattagta cactatcaac
     1801 caaaaggagt atccgaatga ctgacctgaa caccctgttt gccaacctca aacaacgcaa
     1861 tcccaatcag gagccgttcc atcaggcggt tgaagaagtc ttcatgagtc tcgatccgtt
     1921 tttggcaaaa aatccgaaat acacccagca aagcctgctg gaacgcatcg tcgaacccga
     1981 acgcgtcgtg atgttccgcg taacctggca ggacgataaa gggcaagtcc aagtcaaccg
     2041 gggctaccgc gtgcaaatga gttccgccat cggtccttac aaaggcggcc tgcgcttcca
     2101 tccgaccgtc gatttgggcg tattgaaatt cctcgctttt gaacaagtgt tcaaaaacgc
     2161 cttgaccacc ctgcctatgg gcggcggcaa aggcggttcc gacttcgacc ccaaaggcaa
     2221 atccgatgcc gaagtaatgc gcttctgcca agcctttatg accgaactct accgccacat
     2281 cggcgcggac accgatgttc cggccggcga catcggcgta ggcgggcgcg aaatcggcta
     2341 cctgttcgga caatacaaaa aaatccgcaa cgagttttct tccgtcctga ccggcaaagg
     2401 tttggaatgg ggcggcagcc tcatccgtcc cgaagcgacc ggctacggct gcgtctattt
     2461 cgcccaagcg atgctgcaaa cccgcaacga tagttttgaa ggcaaacgcg tcctgatttc
     2521 cggttccggc aacgtggcgc aatacgccgc cgaaaaagcc atccaactgg gcgcgaaagt
     2581 gctgaccgtt tccgactcca acggcttcgt cctcttcccc gacagcggta tgaccgaagc
     2641 gcaactcgcc gcgcttatcg aactgaaaga agtccgccgc gaacgggttg ccacctacgc
     2701 caaagagcaa ggtctgcaat actttgaaaa ccaaaaaccg tggggcgtcg ccgccgaaat
     2761 cgccctgccc tgcgcgaccc agaacgaatt ggacgaagaa gccgccaaaa ccctgttggc
     2821 aaacggctgc tacgtcgttg ccgaaggtgc gaatatgccg tcgactttgg gcgcggtcga
     2881 gcaatttatc aaagccggca tcctctacgc cccgggaaaa gcctccaatg ccggcggcgt
     2941 agcaacttca ggtttggaaa tgagccaaaa cgccatccgc ctgtcttgga ctcgtgaaga
     3001 agtcgaccaa cgcctgttcg gcatcatgca aagcatccac gaatcctgtc tgaaatacgg
     3061 caaagtcggc gacacagtaa actacgtcaa tggtgcgaac attgccggtt tcgtcaaagt
     3121 tgccgatgcg atgctggcgc aaggcttcta agcaaacgcc gccgctccgc aaacaaaatg
     3181 ccgtccgaac cgcaaatgct gttcagacgg catttcatta tccgcccgtt cgaatcgggt
     3241 gatactattt gtatccagaa ttgttggata gtttgggagt ggagaggaag aaatgattat
     3301 ggaccaatac tatatggagt taaagaataa gttatctaac agaccaatcc tactggataa
     3361 tactaatgat tttctttttg ttttagtaaa tacagtgaaa gctatgatag aaaatactga
     3421 taaaagtcag ctttcagagt tagataaaat tttagacggt gtcacaagtc aagaattgaa
     3481 acttgcctat gatttttgtc aagggaaatt cggtcaagca ggtttttctt atcgtaggca
     3541 cccgaattat ttttatttgt ccagtttaat tgcgactttt ccagaatttg agttgtctaa
     3601 agcagaccga gattacctca aaggaatcat aaattttgat aactaccttt tatatgagtt
     3661 agattagtct ttaatcacct caaaacccta tttattcaaa aggagaaaaa atgattgagc
     3721 tgaaattagt agatgagagc agttttcagg cagtgctgga tttgaaaata tctgaagctg
     3781 atgaacgagc acgttttgtg gctccaaatg tacgctcttt agctgacgca tggctctact
     3841 gggaaaatga agatgtgttt ccgagggcaa tctattggga taagcaagtg gttggctttc
     3901 tcctgctgga aatagacaag gatgaagcag aatactttat ctggcggata atgattggtc
     3961 ag
//
LOCUS       DAXHRI010000165         3859 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXHRI010000165 DAXHRI010000000
VERSION     DAXHRI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.165"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="ACFM75_001863"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="ACFM75_001863"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC6256357.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGIAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDDGGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="ACFM75_001864"
     CDS             1439..1924
                     /locus_tag="ACFM75_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC6256358.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM75_001865"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM75_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC6256359.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPSRQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          788 a          940 c         1076 g         1055 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcatcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaagttttag atgacggcgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgcccaa
     2041 acggcgtttc ttatcggctt cgtattcctg atagttgccg tcgaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccaac ggtcgtgaga
     2161 aatcaccatc acgctgccgg caaattccaa caatgcgtct tccagcgcgc gcagggtttc
     2221 tacatcaagg tcgttagacg gttcgtccag cagcaacaca ttgccgccgc tcaacaaggt
     2281 ttttgctaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccctgcga ttttgctttg
     2341 gtcgctgcct ttaaagttga agcgtcccaa atattggcgg gagggaattt caaactgtcc
     2401 gacctgtaaa atgtcgcggc cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttgaacagc gtagatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttatcg ccgaacgatt tggaaacatt tacaaattca atcacttcgt tacccaaacg
     2701 ctcggcaacg ggaataaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcctca aaacgcgcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcctccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt ccggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt gatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacgcccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAXHRI010000166         3846 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXHRI010000166 DAXHRI010000000
VERSION     DAXHRI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.166"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            235..2115
                     /locus_tag="ACFM75_001866"
     CDS             235..2115
                     /locus_tag="ACFM75_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC6256360.1"
                     /translation="MPYFALFDDAVSGRAKRYQNYVESRFFRPEELDALDGALQKGWQ
                     KGLHSVLFADYEFGLPLTGVESERGGNLALHWFADCTDTDAASWLARHSDDLPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRAENGRCTLLDRHLCRLKTSARALN
                     LPLPDGCENQIKQYIADLPDGVFRIKALLASDGISLSRAVLNRLTDKQRVIISPAVLP
                     AQNYLRHFKTTHRALFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKYQGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
     gene            complement(2174..3691)
                     /locus_tag="ACFM75_001867"
     CDS             complement(2174..3691)
                     /locus_tag="ACFM75_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6256361.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAEKLDLPAPVLENVGRFRKKAEGLTGWRFSGGISPAVNRNANNAAPQYCWQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSCYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT          907 a         1287 c          866 g          786 t
ORIGIN      
        1 ttggagctga tcttgctttt cgcctaattc tacgttttca aacggttgca gctggtggtc
       61 tgccataaag gtctccttat tgtatttcag gttggaaatc ggaatttgtt ttcacaattt
      121 cacaccttcg cccccgcttt ctctacataa aattacattt tgccgatatt tgccgaatcg
      181 tctgaaaata tgtgtaaaaa gagcgtataa tcaaaacatt tgccccgaat tgccatgccc
      241 tatttcgccc tgtttgacga tgccgtaagc ggccgcgcaa aacgctatca aaattatgta
      301 gaaagccgtt ttttccgtcc cgaagaactc gatgctttgg acggcgcgct gcaaaagggc
      361 tggcaaaaag ggctgcattc ggtgttgttt gcagactacg aattcggttt gccgctgacg
      421 ggggttgagt ccgagcgcgg cggcaacctt gccctgcact ggtttgccga ctgcaccgac
      481 accgatgccg caagctggct tgcccgacac tcagacgacc ttcccgccgg catttccacg
      541 ccgcaatcct ccgtatccga aaccgattac ctcgaccgca tccgccaaat ccacgaagcc
      601 atccggcgcg gcgacaccta tcaaatcaac tacaccactc gcctgcacct gcaagcctac
      661 ggcaatcccg tcagcctcta ccgccgcctg cgccagcccg tcccctatgc cgtcttgtcc
      721 cacctgcccg atgcggaggg gcaatccgcg tggacgctgt gtttctcgcc cgaactcttc
      781 ctcaaaatcg gttcagacgg caccatcagc accgaaccga tgaaaggcac cgcgccgatt
      841 ttgggcgacg gacaagacga acgccgcgcc gccgagttgc aagccgaccc gaaaaaccgc
      901 gccgaaaacg tgatgattgt cgatttgctg cgcaacgacc tcggcaaaat cgcccaaacc
      961 ggcaaagtat gcgtgcccga gccgtttaaa gtatcacgtt tcggcagcgt ttggcagatg
     1021 accagcacca tccaagccca agccctgccg cacacctcgt tcgccgacat cctccgcgcc
     1081 gccttcccct gcggcagcat caccggcgcg cccaaaaaaa tgagtatgca gattatcgaa
     1141 tcgctcgaaa ccgaagcacg cggactttat acgggcagca tcggctattt gaacccgtgt
     1201 tccggcggct tggggtttga aggcacgttc aacgtcgtta tccgcacctt atcgctcaaa
     1261 cccgtttcgg cttcagacgg cattgtttca ggcatcggcg gcccggattc aaacgcgcaa
     1321 gcccgaacgg cagggcaagg cggggcaacg ccgcatccgt ttgactccaa tccgccgtat
     1381 cggggcgtgt acggtgtcgg ttccggcatc gtcatcgaca gcgaccccgc cgccgaatat
     1441 cgcgaatgcg gctggaaagc ccgtttcctc aacgaattgc gccccgactt cggcattttt
     1501 gaaaccctgc gcgcggaaaa cggacgctgc accctgctcg accgccacct atgccgtctg
     1561 aaaacctccg cacgcgccct caacctgccc ctgcccgacg gttgcgaaaa tcaaatcaaa
     1621 caatacattg ccgacttgcc cgacggcgtg ttccgcatca aagccctgct cgcttcagac
     1681 ggcatcagcc tgtcccgcgc cgttttaaac cgtctgaccg acaaacaacg cgtcatcatt
     1741 tcgcccgccg tcctgcccgc acaaaactac ctgcgccact tcaaaaccac acaccgcgcc
     1801 ctcttcgacc aagcgtggca aaccgccgaa acacaaggcg cgttcgacag cctgtttttc
     1861 aactcggacg gcattctgct cgaaggcggc agaagcaacg tgttcgtcaa ataccaagga
     1921 caatggctta cgccctcctt agatttagac attttaaacg gcataatgcg ccaagccgta
     1981 ttggacgaac cgcaaaaata tttgcaaaca aatcaagtaa tcgaaacaca catcacacaa
     2041 aaaacactgc aagaagccga agaaatccgc ctctccaacg ccctgcgcgg cgtatttgcc
     2101 gccgcccttg cctgaacgcg caaaaatgcc gtccgaacct gtttccaaag ttcggacggc
     2161 attatcccac cattcaaaac cgccaatccg ccgacacaaa cacctcgctg ttgcggcgtt
     2221 tcgcatacgg cacattactt tccgtcctgc cgaaacgata attcaacgcg ggcacgatac
     2281 ctttgtacga caacttgtcg tggctcaaag ccagcgagac attccattcg cggttgcgtt
     2341 gcgcctctgt cgagaaagcc gcaacgccct tatagttgcg gcgggcataa gacgcggaaa
     2401 cccgactatt caaaccgccc aactgccgcc actcctgcgc ccaaccggca taaacaccgt
     2461 tgcgccggta ggcggcatta ttgaccgcgc cgcccaccgt ttcgcgtttc ggcacaaacc
     2521 gcacaaactg ccagccgccg aacacagttg ccgattcgcc caaacgtttt gccgacgaaa
     2581 cataaaaccc gtcctgcctg ccgttattgt attccgccct atcctgttcg cggtagcgtt
     2641 ggcggtaatg ttccagcgcg accgaaaatt gccatcccgg gtttggtcgg taagtatggg
     2701 acagctgcac gccgactccg tgcgccagca tatacggcgg caggcggcgg ttgtttaccc
     2761 gttttgtttt cgcatcaaag ccgtcgctgc ccgacaactg cacctgataa aacggcaaaa
     2821 tccccgccgt ctgccgtgca tttttatact gccaacccaa atacgccctg ccgaacccgt
     2881 catcataagc tgatttttta ctgaaataac agctcgtgcc gccgatattg gaacggaaca
     2941 acaaataatg attgtccgcc aacgccgtca gcttttccgc ctcgatttca taattcaacc
     3001 ctgccgcccg ctccgcccgg ctgacactgc atatctgccg gcctccgttt tgccagcaat
     3061 actgcggcgc ggcattattg gcatttctat tgaccgccgg gctgataccg cccgaaaaac
     3121 gccagcccgt cagcccctcc gcttttttcc gaaaacgccc cacattttcc aaaaccggcg
     3181 ccggcaaatc caatttttcc gcctcggcaa aatgcctttc tgccgacttc agccggaaat
     3241 cgtcaaactc cgccgccgcc aaatccagca aaatccgctc gtctgccgca ttttccccgt
     3301 gcagttcccg ataccgcgcc accgcctccg ccggccttcc cgccaatttc gccagcaaag
     3361 cccgcgccct gccgtacaaa accgcgtcat aatccggcag cttggcatac aaatccgcca
     3421 acgaagcgat taaatccgcc tgattgccgt tgagcgcgtc gcgcaaacta tgttccaaca
     3481 ttttcgggtg cgccaacaaa aaatccccgt caaccacgcg cggggcatca ttttcaactt
     3541 tccaatccga ttccgcccat ttatccgaca ccgaccgctg cacctgcaac aatgccttgt
     3601 catccaaaat cgcgggcgca tccgccccat aggcggcaga aacacctgcc gcacaccaaa
     3661 caaccaaaaa accgtatctg aaatacaaca taccctgtca tttacctttc tggcaaacac
     3721 gccgccgaag cacgtcaaac catccgaaaa acaggcagaa acccgtgaaa accggctttg
     3781 ccgtctgaaa gcgggcaggc aaaaaaccgc cgcccgattt tcaaagggcg gatttcacat
     3841 ttatag
//
LOCUS       DAXHRI010000167         3840 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXHRI010000167 DAXHRI010000000
VERSION     DAXHRI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.167"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            330..1451
                     /gene="dnaJ"
                     /locus_tag="ACFM75_001868"
     CDS             330..1451
                     /gene="dnaJ"
                     /locus_tag="ACFM75_001868"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6256362.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEVCDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1647..3665
                     /locus_tag="ACFM75_001869"
     CDS             1647..3665
                     /locus_tag="ACFM75_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC6256363.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(3709..>3840)
                     /locus_tag="ACFM75_001870"
     CDS             complement(3709..>3840)
                     /locus_tag="ACFM75_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6256364.1"
                     /translation="VLIWNDPAIGIGWPLQTAPLLSPKDLAGKTWAQAEKLRLTLSR"
BASE COUNT          810 a          867 c         1154 g         1009 t
ORIGIN      
        1 gtcggcgcgc ccgcaccaaa aggcccagcc aaaccagtgc tgcccgcgga taaaaaataa
       61 aatagggggg aagtctgcag tcgcatcaaa tgccgtctga acatgcgttc gggcggcgtt
      121 tttataacaa aaacacttca tggcggttgg ttttatgcct gtctaagttt ttgtgtcgtg
      181 catacctgaa gatttcagac ggcatcggtt tatgctgtct gaaaagtgta ttccgtttca
      241 gtttgtaagc tatggcagtc tgtttgtctt gtgttttgcg caattgccct tattttgagc
      301 cgtgatttta ttttgaatta gatgaaaaaa tgagtaatca agatttttat gcgacgctgg
      361 gtgtggcaag aacagctacc gatgatgaga ttaaaaaagc ctaccggaaa ttggcgatga
      421 aataccatcc cgaccgcaat cctgacaata aagaggcgga agagaagttt aaagaagtac
      481 aaaaggcgta tgaaactttg tccgacaagg aaaagcgcgc tatgtacgac cagtatggtc
      541 atgcggcgtt tgaaggcggc ggacaggggg gcttcggagg gtttggcgga tttggcggtg
      601 cgcagggttt tgactttggg gatattttca gccaaatgtt tggaggcggt tcggggcgcg
      661 cccagcctga ttatcagggt gaggacgttc aagtcggtat cgaaatcacg cttgaagaag
      721 ccgcaaaagg tgtgaagaaa cgcatcaata ttccgactta tgaagtgtgt gatgtctgta
      781 acggcagtgg cgcgaaaccg gggacatccc cggaaacctg cccgacttgc aaaggttcgg
      841 gtacggtgca catccagcag gcgattttcc gtatgcagca gacttgtccg acctgccacg
      901 gtgcgggcaa acacattaaa gaaccttgcg tcaaatgccg tggcgcgggg cggaataagg
      961 cggtcaagac ggtggaagtc aatattcccg ccggtatcga tgacgggcag cgtatccgtt
     1021 tgagcggcga aggcggaccg ggtatgcacg gtgcgcctgc tggcgacctg tatgtaaccg
     1081 tccgcattcg ggcgcataag attttccaac gcgacggtct ggacttgcat tgcgaactgc
     1141 cgatcagttt tgccacggct gctttgggcg gggagttgga agtgccgacc ttggacggaa
     1201 aggtcaagct caccgtcccc aaagaaaccc aaaccggcag gaggatgcgc gtgaagggta
     1261 agggtgtcaa atctttacgc agcagcgcga ccggcgattt gtactgccat attgttgtcg
     1321 aaacgcctgt caatttgacc gaccgtcaaa aagagctttt ggaagaattt gagcggattt
     1381 ctaccggctt ggaaaaccaa acaccgcgca agaaatcgtt tttagacaag ctgcgcgatt
     1441 tgtttgattg attttaaggt tcggaaacaa gcagccgtat cggggaatct ccctgatacg
     1501 gctgtttttg tttgtttaaa aatagttttt attttcaatg gggtatgggg cagggtggga
     1561 taactgtttt taactgttct ttttaaaact tgacatcatg gcgcgatgcc aacaatatgt
     1621 gaacgtctgt tgtcaaagga agaataatga ataaatcttt atccgggtcg gtagaagaat
     1681 accgcgaact gacgctccga ggcatgatac tcggtgcatt gatcactgta atttttactg
     1741 cgtccaacgt ttacctcggt ttgaaagtcg ggctgacctt tgcctcgtcg attccggcgg
     1801 cggtaatttc gatggcggtt ttaaagtttt tcaaaggcag caatattttg gaaaacaaca
     1861 tggtgcagac ccaagcttcg gcggcgggta cgctttcgac catcatcttc gtcctgcccg
     1921 gtttgctgat ggcgggctac tggagcggtt tcccgttctg gcagacgacg cttttatgta
     1981 ttgccggcgg gattttgggg gtaattttta ccattccgct gcgttacgcg atggtggtga
     2041 aaagcgattt gccttatccg gaaggtgtgg cggctgctga aattttgaaa gtgggcggtc
     2101 atgaagaagg ggataaccgt cagggaggca gcggcatcaa agagctggcg gccggcggtg
     2161 cgttggcggg attgatgagc ttttgcgccg gaggtctgcg cgtgattgcc gacagcgcga
     2221 gttattggtt taaaagcggt acggcgattt tccaactgcc gatgggcttt tcactggcat
     2281 tgttgggcgc gggctatttg gtcggactga cgggcggtat cgccatcctg ttgggcattt
     2341 cgattgcttg gggcattgcc gtgccgtatt tctcctcaca cattccgcaa ccttccgata
     2401 tggaaatggc ggcgtttgcg atgaagctgt ggaaggagaa agtgcgtttt atcggtgcgg
     2461 ggactattgg cattgcggcg gtttggacgc tgttgatgct gctcaagccg atggtggaag
     2521 gcatgaagat gtcgttcaag agttttggcg gcggtgcgcc cgctgcggaa cgcgccgaac
     2581 aggatttgtc gcctaaagcc atgatttttt gggtgctggc gatgatgttt gttttaggcg
     2641 tgtcgtttta ccactttatc ggcgattcgc acattacggg cggcatggct tggcttttgg
     2701 tggtcgtttg cacgcttttg gcttccgtca tcggcttttt ggtcgccgcc gcctgcggtt
     2761 atatggcagg tttggtcggc tcgtcttcca gcccgatttc cggcgtgggc atcgtgtccg
     2821 tcgtcgttat ttcactggtt ttgctgctgg taggcgaatc cggaggtttg ttggcggatg
     2881 aggctaaccg caaatttttg ctggcactga ctttgttttg cggctcggca gtaatctgcg
     2941 tggcttcgat ttccaatgac aacctgcaag acttgaaaac cggctacctg ctcaaagcca
     3001 cgccttggcg gcagcaagtc gccctgatta tcggctgtat cgttggtgcg ctggttattt
     3061 cgcccgtgtt ggaactgctt tacgaagcct acggctttac cggcgcaatg ccgcgcgaag
     3121 gcatggatgc ggcgcaggct ttggcagcac ctcaagcgac tttgatgacg accatcgcgt
     3181 cgggcatttt cgcccacaac ctcgaatggg tctacatctt taccggcatc gcgattggcg
     3241 cggtgttgat tgccgccgat ttggtgttga aaaaatcatc aggcggcaaa cttgccctgc
     3301 ccgtccttgc ggtcggtatg ggtatttatc tgccgccgtc cgtcaatatg cccatcgtgg
     3361 caggcgcggt gttggcggcg gtgttgaaac acatcatcgg taaaaaagcg gaaaaccgcg
     3421 aaggccgtct gaaaaacgcc gagcgcatcg gaaccttgtt ctccgccggc ctgattgtcg
     3481 gtgaaagcct gatcggtgtg attatggcgt ttattattgc cttctccgtg accaacggcg
     3541 gctcggatgc gccgcttgcg ttgaatctgc aaaactggga tgccgccgct tcttggttgg
     3601 gtttggcgtt cttcgttacc gggatgtttt tctttgcaca gcgcgtactg aaggcgggta
     3661 ggtagcctgt cggagaaaat gccgtctgaa acgttcagac ggcatttttt atcgggaaag
     3721 tgtaaggcgg agcttttcgg cttgcgccca cgttttgccg gcaaggtctt tgggcgacag
     3781 cagcggcgcg gtttgaagcg gccagcctat gccgattgcc gggtcgttcc atattaaaac
//
LOCUS       DAXHRI010000168         3638 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXHRI010000168 DAXHRI010000000
VERSION     DAXHRI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.168"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            264..3467
                     /gene="nalP"
                     /locus_tag="ACFM75_001871"
     CDS             264..3467
                     /gene="nalP"
                     /locus_tag="ACFM75_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HFC6256365.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHT
                     GDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFI
                     FSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     GTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT          982 a          997 c          975 g          684 t
ORIGIN      
        1 ctagggaaat tcaaattaag ttagaattat ccctatgaga aaaagccgtc taagccggta
       61 taaacaaaat aaactcattg agctatttgt cgaaagttca aatttccatt ttaaaacaat
      121 tagtaaaatc gagtttatcc tagttgtcca agacaacccc tataataata taattcaaaa
      181 tataaaaatg ggttacatct aaacattacg gaatttttat tccctcgcct gaattctatt
      241 gtcagattca aggagacctc atcatgcgaa cgaccccaac cttccctaca aaaactttca
      301 aaccggctgc catggcgtta gctgttgcaa caacactttc tgcctgctta ggcggcggcg
      361 gcggcacttc tgcgcccgac ttcaatgcag gcggcaccgg tatcggcagc aacagcagag
      421 caacaacagc gaaatcagca gcagtatctt acgccggtat caagaacgaa atgtgcaaag
      481 acagaagcat gctctgtgcc ggtcgggatg acgttgcggt tacagacagg gatgccaaaa
      541 tcaatgcccc ccccccgaat ctgcataccg gagactttac aaacccaaat gacgcataca
      601 agaatttgat caacctcaaa cctgcaattg aagcaggcta tacaggacgc ggggtagagg
      661 taggtatcgt cgacacaggc gaatccgtcg gcagcatatc ctttcccgaa ctgtatggca
      721 gaaaagaaca cggctataac gaaaattaca aaaactatac ggcgtatatg cggaaggaag
      781 cgcctgaaga cggaggcggt aaagacattg aagcttcttt cgacgatgag gccgttatag
      841 agactgaagc aaagccgacg gatatccgcc acgtaaaaga aatcggacac atcgatgtgg
      901 tctcccatat tattggcggg cgttccgtgg acggcagacc tgcaggcggt attgcgcccg
      961 atgcgacgct acacataatg aatacgcatg atggaaccaa gaacgaaata atgtctgcag
     1021 ccatccgcaa tgcatgggtc aagctgggcg aacgtggcgt gcgcatcgtc aataacagtt
     1081 ttggaacaac atcgagggca ggcactgccg accatttcca aatagccaat tcggaggagc
     1141 agtaccgcca agcgttgctc gcctattccg gcggtgataa aacagacgag ggtatccgcc
     1201 tgatgcaaca gagcgattac ggcaacttgt cctaccacat ccgtaataaa aacatgcttt
     1261 tcattttttc ggcaagcaat gacgcacaag ctcagcccaa cacactgacc ctattgccat
     1321 tttatgaaaa agatgctcaa aaaggcatta tcacagtcgc aggcgtagac cgcagtggag
     1381 aaaagttcaa tggctccaac cattgcggaa ttactgccat gtggtgccta tcggcaccct
     1441 atgaagcaag cgtccgtttc acccgtacaa acccgattca aattgccgga acatcctttt
     1501 ccgcacccat cgtaaccggc acggcggctc tgctgctgca gaaatacccg tggatgagca
     1561 acgacaacct gcgtaccacg ctgctgacaa cggctcagga catcggtgca gtcggcgtgg
     1621 acagcaagtt cggctgggga ctgctggatg cgggtaaggc catgaacgga cccgcgtcct
     1681 ttccgttcgg cgactttacc gccgatacga aaggtacatc cgatattgcc tactccttcc
     1741 gtaacgacat ttcaggcacg ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc
     1801 acggcaacaa cacctatacg ggcaaaacca ttatcgaagg cggttcgctg gtgttgtacg
     1861 gcaacaacaa atcggatatg cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg
     1921 catccggcgg tagcctgaac agcgacggca ttgtctatct ggcagatacc gaccgatccg
     1981 gcgcaaacga aaccgtgcac atcaaaggcg atctgcagct gggcggcgaa ggtacgctgt
     2041 acacacgttt gggcaaactg ctgaaagtgg acggtacggc gatgaccggc ggcaagctgt
     2101 acatgtcggc acgcggcaaa ggggcaggct atctcaaccg taccggacaa cgtgttccct
     2161 tcctgagtgc cgccaaaatc gggcgggatt attctttctt cacaaacatc gaaaccgacg
     2221 gtggtctgct ggcttccctc gacagcgtcg aaaaaacagc gggcagtgaa ggcgacacgc
     2281 tgtcctatta tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg gcacattccg
     2341 cgcccgccgg tctgaaacac gccgtagaac agggcggcag caatctggaa aacctgatgg
     2401 tcgaactgga tgcctccgaa tcatccgcaa cacccgagac ggttgaaact gcggccgccg
     2461 accgcacaga tatgccgggc atccgcccct acggcgcaac tttccgcgca gcggcagccg
     2521 tacagcatgc gaatgccgcc gacggtgtac gcatcttcaa cagtctcgcc gctaccgtct
     2581 atgccgacag taccgccgcc catgccgata tgcagggacg ccggctgaaa gccgtatcgg
     2641 acgggttgga ccacaacggc acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg
     2701 gaacgtggga acagggcggt gttgaaggca aaatgcgcgg cagtacccaa accgtcggca
     2761 ttgccgcgaa aaccggcgaa aatacgacag cagccgccac actgggcatg ggacgcagca
     2821 catggagcga aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt gcaggcatac
     2881 ggcacgatgc gggcgatatc ggctatctca aaggcctgtt ctcctacgga cgctacaaaa
     2941 acagcatcag ccgcagcacc ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc
     3001 tgatgcagct gggcgcactg ggcggtgtca acgttccgtt tgccgcaacg ggagatttga
     3061 cggtcgaagg cggtctgcgc tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca
     3121 gtgctttggg ctggagcggc aacagcctca ctgaaggcac actggtcgga ctcgcgggtc
     3181 tgaagctgtc gcaacccttg agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac
     3241 gcgacctgaa cggacgcgac tacacggtaa cgggcggctt taccggcgcg actgcagcaa
     3301 ccggcaagac gggggcacgc aatatgccgc acacccgcct ggttgccggt ctgggcgcgg
     3361 atgtcgaatt cggcaacggc tggaacggct tggcacgtta cagctacgcc ggttccaaac
     3421 agtacggcaa ccacagcgga cgagtcggcg taggctaccg gttctgacgg acaggaagca
     3481 gacagccgca aagatcacgg tctttgcggc tgtttcttat gaaaagaaaa ccctattcca
     3541 attgcctgct tctattgttt caagacttct tccaaagatt cggcatcaat cagatgtata
     3601 gcggattaac aaaaatcagg acaaggcggc gaagccgc
//
LOCUS       DAXHRI010000169         3617 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXHRI010000169 DAXHRI010000000
VERSION     DAXHRI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.169"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..107)
                     /locus_tag="ACFM75_001872"
     CDS             complement(<1..107)
                     /locus_tag="ACFM75_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6256366.1"
                     /translation="MSTFFRQTAQAMIARHIDRFPLLKLDQVIDWQQIE"
     gene            complement(331..2559)
                     /locus_tag="ACFM75_001873"
     CDS             complement(331..2559)
                     /locus_tag="ACFM75_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFC6256367.1"
                     /translation="MRSAVLFIYFFLLNIFLFQPAVIHIQSNYILFMSIVSAPLPALS
                     ALIILAHYHGIAANPADIQHEFCTSAQSDLNETQWLLAAKSLGLKAKVVRQPIKRLAM
                     ATLPALVWCDDGNHFILAKTDGEGEHAQFLIQDLVTNKSAVLSFAEFSNRYSGKLILV
                     ASRASVLGSLEKFDFTWFIPAVIKYRRLFFEVLVVSVVLQLFALITPLFFQVVMDKVL
                     VHRGFSTLDVVSVALLVVSLFEIVLGGLRTYLFAHTTSRIDVELGARLFRHLLSLPLS
                     YFEHRRVGDTVARVRELEQIRNFLTGQALTSVLDLAFSFIFLAVMWYYSSTLTWVVLA
                     SLPAYAFWSAFISPILRTRLNDKFARNADNQSFLVESITAVGTVKAMAVEPQMTQRWD
                     NQLAAYVASGFRVTKLAVVGQQGVQLIQKLVTVATLWIGARLVIEGKLTVGQLIAFNM
                     LSGQVAAPVIRLAQLWQDFQQVGISVARLGDILNAPTENASSHLALPDIRGEITFEHV
                     DFRYKADGRLILQDLNLRIRAGEVLGIVGRSGSGKSTLTKLVQRLYVPEQGRVLVDGN
                     DLALAAPAWLRRQVGVVLQENVLLNRSIRDNIALTDTGMPLERIIEAAKLAGAHEFIM
                     ELPEGYGTVVGEQGAGLSGGQRQRIAIARALITNPRILIFDEATSALDYESERAIMQN
                     MQAICANRTVLIIAHRLSTVKTAHRIIAMDKGRIVEAGTQQELLAKPNGYYRYLYDLQ
                     NG"
     gene            complement(3228..3617)
                     /locus_tag="ACFM75_001874"
                     /pseudo
     CDS             complement(3228..3617)
                     /locus_tag="ACFM75_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1026 a          952 c          817 g          822 t
ORIGIN      
        1 tgttcgatct gctgccaatc aatcacttga tccaacttca atagcgggaa gcggtcgatg
       61 tgtctggcaa tcatggcttg tgcggtttgc cggaagaagg tgctcatgag aaatctccta
      121 aatgtcttgg tgggaattta ggggattttg gggaattttg caaaggtctc tagatgagtg
      181 aaaaagaagt gcaggctgcc taaaaagaca gaaaaagtct ttccggcagc ctgcactttg
      241 gtttcatttc agtcagtaaa cccagtaaac gacggtctaa aaacgcagaa cgttacgaaa
      301 aaagcagcct acacgcccat cccccgcctt ctacccgttc tgtaaatcat acagatagcg
      361 gtaatatccg ttcggcttcg ccagcaattc ctgctgtgtt cccgcttcca caatcctgcc
      421 tttatccatg gcaatgatcc ggtgtgccgt tttaacggtg gacagacggt gggcgataat
      481 cagcaccgtc cggttggcgc aaatggcctg catgttctgc ataatcgctc gttcactttc
      541 ataatccagc gcgctggtgg cttcgtcaaa aatcagaatg cgcggattgg tgattaacgc
      601 gcgggcaatc gcaatacgct gccgctgtcc gcccgacaag ccggcccctt gttcgcccac
      661 cacggtgccg tagccttccg gcagctccat aataaactcg tgtgcgcccg ccagtttggc
      721 tgcttcgata atgcgttcca gcggcatacc cgtatccgtc agcgcgatat tgtcgcgtat
      781 gctgcggttg agcagcacat tctcctgcaa gaccacgccg acctgccgcc gcagccaggc
      841 aggagcggcc aaagccaaat cgttgccgtc caccaacacc cgtccctgct ccggtacata
      901 cagacgctgc accaatttgg tgagtgtgga tttgcccgac cccgaacgtc ccacaatccc
      961 cagcacttcc cccgcccgaa tccgcaggtt caaatcctgc aaaatcagcc tgccgtccgc
     1021 cttatagcgg aaatcgacat gttcgaacgt aatctccccc cggatatcgg gcaaagccaa
     1081 atgcgaagac gcattctcgg tcggcgcatt cagaatatcc cccaaacgcg ccaccgaaat
     1141 ccccacctgc tggaaatcct gccacaactg cgccaaacgg ataacaggcg ccgccacctg
     1201 tcccgagagc atattaaacg caatcagctg ccccaccgtc agcttgccct caattaccag
     1261 ccgtgcgcca atccacaacg tcgccaccgt caccagcttc tgaatcagct gcaccccctg
     1321 ctggccgacc accgccaact tcgttacccg aaatcccgaa gccacataag ccgccaactg
     1381 attgtcccaa cgctgcgtca tctgcggctc caccgccatc gcctttaccg tacccaccgc
     1441 agtgatgctt tctactaaaa acgactggtt gtctgcattg cgcgcgaact tatcgttcag
     1501 acgcgtccgc agtatcggac tgataaatgc cgaccaaaac gcataggcag gcaacgaagc
     1561 caataccacc caagtcagag tggagctgta ataccacatc accgccagaa agataaacga
     1621 aaacgccaaa tccaacaccg aagtcagcgc ctgaccggtc aggaaattgc gaatctgctc
     1681 caattcccgc acccgagcca ccgtatcacc cactcgtctg tgctcgaaat aggataaagg
     1741 cagggaaagc agatgccgga acaaacgcgc gcccaattcc acatcaatac gtgaagtcgt
     1801 atgtgcaaac agatacgtcc gcaaaccgcc caacacaatc tcaaacagcg acaccaccaa
     1861 caaagccacc gacaccacat ccaaagtaga gaatccccga tgtaccagca ccttgtccat
     1921 caccacttgg aaaaacagag gcgtaatcag cgcaaacagc tgcaacacca ccgacaccac
     1981 caatacttca aaaaacaacc ggcggtattt gattaccgcc ggaataaacc aggtaaagtc
     2041 aaacttttcc aaactgccca ataccgaagc gcgggaagca accaatatca gtttgcccga
     2101 atatctgtta gaaaattcgg caaaagacaa taccgcagac ttattcgtaa ccaaatcctg
     2161 tatcaaaaat tgggcatgct caccctcacc gtctgttttg gccaaaatga aatggttgcc
     2221 gtcatcacac cataccaatg cgggtaaagt cgccatagcc aaacgtttaa taggctggcg
     2281 gactaccttt gccttcaatc ccaaagattt ggcggctaac agccattgcg tttcatttaa
     2341 atcgctctgt gcggaagtac aaaattcatg ctgtatatcg gcaggattgg cggcaatgcc
     2401 gtggtaatgg gcgaggatga tgagggcgga aagggcgggg agcggtgcgg atacgataga
     2461 cataaataaa atatagttag attggatgtg gataacggct ggctggaaaa ggaatatatt
     2521 aagtagaaag aaatatataa ataaaacagc agaacgcatt gtaaggatat atatgggaat
     2581 tgtaaagaga aagtatggaa aagttctcgt ttcaggaagg taaaacggct taggaatcga
     2641 gttagatgag gatgcctcgc acctctcgtg cctcctgcat accgttaagg cacagggtta
     2701 aggtgcaggc tgctccgaac tctgttgcgg tcgggtaatg ttattttttg tgtttcaggc
     2761 agcctgaaat atctgtatat ttttgtttta aatagatttt aaagattgat aactgttctt
     2821 gacgattttt caagaaagga gtaaatttca agaaaggagt aaagtgactt attatcaatg
     2881 acaagcaacg cgcgaagtga caaggaaaac tatctactta aattctaagg aggcttcgaa
     2941 tatcataaac caatcagaaa catagagata aaaattatgt acaaatataa tcctcttata
     3001 caatttattg cacagttgat tatgtcttat ggagcaagcg tagggtgggc acttgctgcc
     3061 ccacgcgttt catatttcaa ggcagcctga aaccgtgtgg gcataaatgc ctaccctaca
     3121 tcccaaaaaa caagcgcagc ctgcgtgtgt agggtgcgaa ctttcggcag gtagacacgc
     3181 agttttatat tttcaagctg agggatgctt aagaaaagta caaaacatta aaaaataagg
     3241 ggctgtacta gattagccct aaatccacac caatcccgca agatttttag ctgtcgggac
     3301 ggtgtgccga agttaaatcg aaattcgcat tctttcaaga acagcgggaa agatttgcga
     3361 tcaattccgt tctatttgcg caagacgcgt tttgcctgat tccaaaagtt ctcaatgccg
     3421 tttatgtggt tctgacggtc agcaaattcc ttggaatggt tgatgcggta atggataaaa
     3481 ccgcttacgt ccaacttgtc gtaactgctc aggctgtcgg tataaacaat gctgtccggc
     3541 atgattttct gtttgataac aggcattaaa gtatcggact tggcattatc tacgacaacg
     3601 gtatagaccc gtccgtt
//
LOCUS       DAXHRI010000170         3532 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXHRI010000170 DAXHRI010000000
VERSION     DAXHRI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.170"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(58..390)
                     /locus_tag="ACFM75_001875"
                     /pseudo
     CDS             complement(58..390)
                     /locus_tag="ACFM75_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(574..1191)
                     /locus_tag="ACFM75_001876"
     CDS             complement(574..1191)
                     /locus_tag="ACFM75_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256368.1"
                     /translation="MSTDISAFWEVIDPEMPDCEVVLTAKGYKLDGAKGLKVVLRITD
                     NAGNTPKSVDYLQLFDKIPLFVEFSDLQTQHIRCSATLESLDLGGLSKSERKRVSSKI
                     SSDTDILNELRGKIVDTDFIFREVSDKALLDGLPELHGGHILVVWHPRSSLSKVDTAK
                     LLDGLRGRLLAELSRYNLKFLNVPLHFLTVEAYACRYGCPTASDT"
     gene            complement(1188..2546)
                     /locus_tag="ACFM75_001877"
     CDS             complement(1188..2546)
                     /locus_tag="ACFM75_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6256369.1"
                     /translation="MDLNVEIKNLGKVAHARVSIRPLTVITGCNSSGKSFITRGLYSI
                     LHTLNQDVVSAYILNVLSLTNSALLRLSDGIPRRSADDDALLADIGLLLEGVRQSVAD
                     RLSPSPLYQPPASQLVLNDLKTAVAELARRLKVGKGTKMQAVKQHYTDIEDYLSDLEF
                     CLSGDVGFYTEAVSRALHSAFSGNFQVADIKRLLSSDSANFSLGDGTEVSIGDKGLGF
                     KLSAAMPGSLQSLKNVVYLESPVYYKMRNALKDARLFRLNMLRRGAVNPVPQYFYDLD
                     EMLEAELPPVNEVFGQIADKVEALINGRLAVTPSGEIVYSERGRQSVPLNMASSGISN
                     IGMIALLLRRNVLDEGSFLFIDEPEMNLHTSWQHAMLDVLLDLSRAGVNVVVATHSLD
                     MLHRLEAKVEADASLLGSVGVCRLSSDGISLEDGSVLGGLRAAKEALGAPYLEVIEER
                     LP"
     gene            complement(2694..3113)
                     /locus_tag="ACFM75_001878"
     CDS             complement(2694..3113)
                     /locus_tag="ACFM75_001878"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6256370.1"
                     /translation="MAYTVYSFEKKFLEKFGVYGLSVLNFRGSMYPLDIHCPKHGNQT
                     VSNATSCLRSKLGCPACGREHQQSKASERLKQSNKSAKPLLILDTTTNETLTFPSVTA
                     AGTALGVHFQQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(3379..3532)
                     /locus_tag="ACFM75_001879"
                     /pseudo
     CDS             complement(3379..3532)
                     /locus_tag="ACFM75_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          922 a          917 c          827 g          866 t
ORIGIN      
        1 atcgagccgt agcaactgta tttttcaccc cgtcgggcaa aaataccaaa actcaaatca
       61 agccgtccgg ataccgtttt cggcggtatc gttttcggca aaataatcac gcatccgggc
      121 attcgatatc gtcagcagtt tgcgcataca tgccgtaacg gcaaccttat acggcttacc
      181 ctcggacagc aggcgttggt agaaatcccg aataagcggt tcaaaacgtg tcgctgccac
      241 ggtagccata tacagtgcct tacgcaccgc agaccttccg ccaaagcagc ggcttttaaa
      301 tttggtttcc ccgccctccc tcgggtgcgg ggcaatgccg gccaaacccg ctatccgttt
      361 gtgcgacagc cgcctcaatt cgggcagcat cgccatccag gtcttacaat cttaccaacg
      421 tataaaacac gcacagtagg ggcaacgcaa tgcagcaacg tctgaaacgc ccgattcata
      481 agggtttcag acaacaaaga aaaaagccgc gcaatcaaac gaaattgcgc ggattctgtc
      541 aatttttgaa aattgcgtgg agttactgcc ccttcaggtg tcggaagccg ttggacatcc
      601 gtacctgcaa gcgtacgctt ccacagtcag aaagtgcaag gggacattta gaaacttaag
      661 gttataccgg ctcaactccg ccaataacct acccctcaat ccgtccaaaa gttttgccgt
      721 atcaaccttg ctcaagctgc tacgcggatg ccaaacaaca aggatatgcc cgccgtgcag
      781 ttcaggcagt ccgtccagca gggctttatc cgaaacttcc ctgaaaataa agtccgtatc
      841 gacgattttg ccgcgcagtt cgttcaaaat atccgtgtct gaactgattt tagatgaaac
      901 acgcttgcgt tcggacttag acagaccgcc caagtccaag gattccagtg ttgcgctgca
      961 tctgatatgc tgtgtctgca agtccgaaaa ctcgacaaat aagggaattt tgtcaaacag
     1021 ttgcagataa tccacacttt tcggcgtatt tcccgcatta tcggttatcc gtagaacgac
     1081 cttcaaaccc tttgcaccgt ccaacttata acccttggca gtcaatacga cttcacagtc
     1141 gggcatctca gggtctatga cttcccaaaa tgccgaaata tccgtactca aggcagccgc
     1201 tcctcgatga cctcaagata aggcgcaccc aaagcttctt tggcagcccg taaaccgccc
     1261 aagacgctgc cgtcttcaag ggatatgccg tctgaagaca gcctgcatac gcccacactg
     1321 cctaataatg acgcatcggc ttccaccttg gcttccagcc tgtgcaacat atccaagctg
     1381 tgcgttgcca ccaccacatt gacacccgcc ctgctcaaat cgagcagaac atccagcata
     1441 gcgtgctgcc aagacgtatg cagattcatc tcaggctcgt cgataaacag gaaactgccc
     1501 tcgtccagca cattgcgccg caacagcagc gcaatcatac cgatgtttga aatccccgac
     1561 gaagccatat tgagcggcac ggactgcctg ccgcgttcgc tgtacacgat ttcccccgag
     1621 ggcgtaacag ccagtctgcc gtttatcagt gcctctacct tatccgcaat ctgcccgaac
     1681 acttcgttca caggaggcag ttcggcctcc aacatttcgt ccaaatcata gaaatactgg
     1741 ggcacaggat tgaccgcccc gcgtcggagc atattcaacc tgaacagcct tgcgtccttt
     1801 aaggcattcc tcatcttgta gtagacgggc gactccaaat agacgacgtt tttcaggctt
     1861 tgcagactgc ccggcatggc tgcggacagt ttgaatccca aacctttgtc cccaatggaa
     1921 acctccgtac cgtcgcccaa tgagaaattt gcactatcgg aggataaaag ccttttgatg
     1981 tcagccactt ggaaatttcc ggaaaatgcc gagtgcaaag ctctgctgac agcttcggtg
     2041 tagaaaccga catctccgga cagacagaac tcaaggtcgg acaggtaatc ctcaatatct
     2101 gtgtaatgtt gtttgaccgc ctgcatcttc gtccccttgc cgactttcag acggcgtgca
     2161 agctcggcaa cagcagtttt caaatcgttt aaaacaagct gcgaggcagg aggttggtac
     2221 aacgggctgg gagacagcct gtctgccacg ctttgcctga caccttccaa aagcagaccg
     2281 atgtcggcca gcagtgcgtc atcatccgcc gaacgtctcg ggatgccgtc tgaaagcctc
     2341 aataaggcgg agttcgtcag ggacagcacg ttcaggatat aggcagaaac aacatcctga
     2401 ttgagcgtat gcaggatact gtacagcccg cgtgtgatga aacttttgcc gctggagttg
     2461 caacccgtga tgacggtaag cgggcgaatg gaaaccctgg catgggcaac cttgccaaga
     2521 ttcttgattt cgacatttaa atccataaaa aacaaactcc tgcaaaagtt aggagtttat
     2581 tatagcacga agtttcgaga ttacggcact actttgtacc tgtttgaaat aaggttgtcg
     2641 gtggacgaac gtccgtcgag cttggttcgt agggatttag agtgttgtga accttaccta
     2701 tcgtacccaa gcactttgta cctgtttgaa ataaggttgt cgggcgacgt tcgacccttg
     2761 agcctgtgat tgatttgttg gaaatgtaca cctaaagcag tgcctgcggc agtaacgctg
     2821 gggaatgtga gtgtttcgtt ggtcgttgtg tcgaggatga ggaggggttt ggcagacttg
     2881 tttgactgtt ttagacgttc agaggctttt gactgttggt gttcccttcc gcaagcaggg
     2941 cagccgagtt tagagcggag acaggatgtc gcattggaga ccgtctgatt cccgtgcttt
     3001 gggcaatgta tatccaacgg gtacatagac cctcggaaat taagaacact cagaccataa
     3061 acaccaaact tctcaagaaa tttcttctca aaactataaa ctgtataagc catgataacc
     3121 tcctagagtt gtcatttatt agaatacagg aaattatgga aaagtcaaaa tttttcgaga
     3181 agtacctgaa agtggtgagt ggtgcatatt gttaaaaagt gtatacgtct gttaagatta
     3241 ggtaaataat ataaactatt tattatatta tatattttcc ttaatgcttt gtttgacaaa
     3301 caacaggcgg aggcgtacaa tgcgccctgt aattagatag ttagaaagga taaaaacaat
     3361 gacttagccc cgcgccccga gcgtagccgt cgttatcgaa ccgatgcctt tgatttgctc
     3421 cgccacttgg gctttgccgt caaaatgcgt gtgggtgtgg tcgtcgattt gtttgtccaa
     3481 ttcgtcaatc agccggtcaa aatgggcaat cagttgtttg acgcttccga ct
//
LOCUS       DAXHRI010000171         3474 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXHRI010000171 DAXHRI010000000
VERSION     DAXHRI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.171"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            185..1060
                     /gene="dapA"
                     /locus_tag="ACFM75_001880"
     CDS             185..1060
                     /gene="dapA"
                     /locus_tag="ACFM75_001880"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC6256371.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGIYRHFKAVAEAAAIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1069..2265
                     /gene="bamC"
                     /locus_tag="ACFM75_001881"
     CDS             1069..2265
                     /gene="bamC"
                     /locus_tag="ACFM75_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC6256372.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGSLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKNPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2322..3254)
                     /gene="pip"
                     /locus_tag="ACFM75_001882"
     CDS             complement(2322..3254)
                     /gene="pip"
                     /locus_tag="ACFM75_001882"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC6256373.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKDWADWESYLICFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIQHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT          918 a         1145 c          778 g          633 t
ORIGIN      
        1 cgacggcacc ggcataaacc ggcgaatata ataaaatgat ttcaaacagg aaaaatcaat
       61 ttccaccata cctttcggcg caaacgcccc acatctgcaa cccgccggat ttcacacctg
      121 cggtaaagta ggctacaatc agaagcatta ccgcccctac accacaactg ataaaaggtt
      181 ctgtatgtta caaggtagct tggttgccct gattaccccg atgaatcaag acggcagcat
      241 ccattacgaa caactccgcg acttaatcga ctggcacatt gaaaacggca cggacggcat
      301 cgtcgccgtc ggcacgacag gcgaatccgc caccctctcc gtcgaagaac acaccgccgt
      361 tatcgaagcc gtcgtcaaac acgttgccaa acgcgtcccc gtcatcgccg gcacaggggc
      421 gaacaacacc gtcgaagcca tcgccctttc ccaagccgcc gaaaaagccg gcgcggacta
      481 caccttatcc gtcgtcccct attacaataa gccctcccaa gaaggcattt accgccattt
      541 caaagccgtt gccgaagccg ccgcgattcc gatgattatc tacaacgtgc ccggccgtac
      601 cgtcgtcagc atgactaacg acactatcct gcgccttgcc gaaatcccca acatcgtcgg
      661 cgtgaaagaa gccagcggca acatcggcag caacatcgaa ctcatcaacc gcgcgcccga
      721 aggcttcgtc gtcctgtccg gcgacgacca caccgcccta ccgttcatgc tctgcggcgg
      781 acacggcgtt attaccgtag ccgccaacgc cgcgcccaag ctctttgccg atatgtgccg
      841 cgccgcgctt caaggcgata tcgcccttgc acgcgaactc aacgaccggc tgattccgat
      901 ttacgacacc atgttctgcg aacccagccc cgccgcaccg aaatgggcgg tatccgccct
      961 gggcagatgc gaaccgcacg tccgcctgcc gctcgttccg ctgacggaag gcggacaggc
     1021 aaaagtccgc gccgccctga aagcctcagg acaactctaa atataactat gccgtctgaa
     1081 ccgttcggac ggcataacgc aacaaacact ttaatatcca tcacacagga tgacacgatg
     1141 acccatatca aacccgtcat tgccgcgctc gcactcatcg ggcttgccgc ctgctccggc
     1201 agcaaaaccg aacagcccaa gctcgactac caaagccggt cgcaccgcct gatcaaactt
     1261 gaagtcccac ccgatttgaa caaccccgac caaggcagcc tctaccgcct gcctgccggt
     1321 tcgggcgccg tccgcgccag cgatttggaa aaacgccgca cacccgccgt ccaacagcct
     1381 gccgatgccg aagtattgaa aagcgtcaaa ggtgtccgcc tcgagcgcga cggcagccaa
     1441 cgttggcttg tcgttgacgg caaatcccct gccgaaatct ggccgcttct gaaagccttt
     1501 tggcaggaaa acggcttcga catcaaatcc gaagaacccg ccatcggaca aatggaaacc
     1561 gagtgggcgg aaaaccgtgc caaaatcccc caagacagct tgcgccgcct attcgacaca
     1621 gtcggtttgg gcggcatcta ctccaccggc gagcgcgaca aattcatcgt ccgtatcgaa
     1681 cagggcaaaa acggcgtttc cgacatcttc ttcgcccaca aagccatgaa agaagtgtac
     1741 ggcggcaaag acaaagacac gaccgtatgg cagccctccc cgtccgatcc caacctcgaa
     1801 gccgctttcc tgacgcgctt tatgcaatat ttgggcgttg acggacagca ggcggaaaac
     1861 gcatcggcaa aaaacccgac cctgcccgcc gccaacgaaa tggcgcgtat cgaaggcaaa
     1921 agcctgattg tctttggcga ctacggcaga aactggcggc gcaccgcgct cgccctcgac
     1981 cgcatcgggc tgaccgtcgt cggtcaaaac accgaacgcc acgctttcct ggttcaaaaa
     2041 gccccgaacg aaagcaatgc agttaccgaa caaaaacccg gcctgttcaa acgcctgctg
     2101 ggcaaaggca aagcggagaa acctgccgaa cagccggaac tgattgtcta tgccgagcct
     2161 gtcgccaacg gctcgcgcat cgtcctgctc aacaaagacg gcagcgcata tgccggcaaa
     2221 gacgcatccg cattattggg caaactccat tccgaactgc gttaaaccat tcaaaacaaa
     2281 agcagcttcc aaacagaagc tgctttttta tgcggaactt tttacaacaa atggggcaaa
     2341 atatcctcaa ctgcctgaac caacgcatcc accaaaggcg gatcgaacgc acgatgccct
     2401 gcctgaacca ccctcaattc cgcttcggga aaggctttcg acagcgccca cgcactctgc
     2461 atcggcgtac acaaatcata ccgcccctgc acgataatgg tcgggatatg ctgtattttg
     2521 ccgatattgt tcaaaatcgc cctatcgccc tgcaaccagc cgccgttgac aaaataatgg
     2581 ttttccaaac gcgcgattgc cagcgaggca taagcatctt catccacttc ctccggctcg
     2641 aaacagatca gatagctttc ccaatccgcc caatccttcg cggcagacag gcacacttct
     2701 tcatcttgat gaaacagcaa cccgtgatac gcctcaatca gccggttccg ccgattttca
     2761 gcaatcggcg cgacaaattt ttgccattgt tccggataaa tccggctcac accgcccgcc
     2821 tcgttcagcc acgccgtttc agacggcctg cacaaaaata tcccgcgcaa caccaatccc
     2881 tttacccgtt ccggatgggt ttgggcataa gccagcgaca aagtgctgcc ccacgaaccg
     2941 ccgaacacca gccatttccc gatacccagc atttcacgga ctttttcaat atccgccacc
     3001 aaatcccaag tcgtattgtc ttccgcacaa gcatacgggc gcgaacgtcc gcaaccgcgc
     3061 tggtcgatga tgacgatgcg gaacacatcg ggattaaaaa aaccccgaca ttcaggcgaa
     3121 gcccccgcgc ccggcccgcc atgtaaaaaa ataaccggca caccgtcagg attgcccgat
     3181 tcctcccaat aaatttgatg aatttcagac acctgcaagt atccgctatg aaaaggctgt
     3241 tttatctcat acatcatctt ctcccatatt gcttctgata atccgaacat tcccacacgc
     3301 aacatcacgc acgagacaca agccgccccg aaaggcaaat gccgaaaaat cgcgatatag
     3361 tgaattaaca aaaatcagga caaggcggcg aagcctcaga cagtacagat agtacggaac
     3421 cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagctaa agcg
//
LOCUS       DAXHRI010000172         3448 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXHRI010000172 DAXHRI010000000
VERSION     DAXHRI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.172"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            43..720
                     /locus_tag="ACFM75_001883"
     CDS             43..720
                     /locus_tag="ACFM75_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC6256374.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTPNNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYST
                     NQDLTNTSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRGDRRTR
                     RTMCQYK"
     gene            744..1355
                     /gene="pilV"
                     /locus_tag="ACFM75_001884"
     CDS             744..1355
                     /gene="pilV"
                     /locus_tag="ACFM75_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC6256375.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHHTLSAVDGDFQVDA
                     IKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGNAPTFSGNAFSSNCDNKAN
                     GDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            1352..2290
                     /locus_tag="ACFM75_001885"
     CDS             1352..2290
                     /locus_tag="ACFM75_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC6256376.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLSAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVISDTTQQNSP
                     FSLKRNGIDKLIPITESLNINYQNFFQVDSALIFQYGIDDLDASAETVVVSSCSKIAK
                     PGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLDDKGK
                     WGNPQLLAKKIRHMKVRYIYVSDCPEDDDAGKEETFKYTDTFNSAKDAVTPAGVEVLL
                     SSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2269..2862
                     /locus_tag="ACFM75_001886"
     CDS             2269..2862
                     /locus_tag="ACFM75_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC6256377.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSEDCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCTANSTNLCIDNKGTEYKKGTQSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2867..3340
                     /locus_tag="ACFM75_001887"
     CDS             2867..3340
                     /locus_tag="ACFM75_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC6256378.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQNIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLKTLSADAGCEAFSNRKK"
BASE COUNT          992 a          690 c          864 g          902 t
ORIGIN      
        1 aatttaatcc actatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacgccca acaataaatg tgactccggc
      301 aagaaggggc agggaatgtt ggctttcggc gacaaaaacg gcaataaggg atatgacaat
      361 gatacggcgg atgttcttct ccgcagtgtg gtattgaatg atgatatcaa tgataagcgg
      421 attaattatg ccttcaacca tatcgctttc ggtcagactc agccgaccgc tgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttattcta ccaatcagga tctcacgaat
      541 acttccaaat ttgtttactc cgacggttat atccaaatcg tgctgacaga tgcgagagca
      601 gtttcagatg ccgataagaa attccgttcg gcggtggttt tgattgacag cagcggcagg
      661 gtcgaagttt gccctagggg tgatcggcgt actcggcgta ctatgtgcca gtataaataa
      721 cagtttcagt tttaaaaatg aatatgaaga ataatgattg cttccgcctg aaagattccc
      781 agtccggtat ggcgctgata gaagtcttgg ttgctatgct cgttctgacc atcggtattt
      841 tggcgttact gtctgtgcag ttgcggacag tcgcttccgt cagggaggcg gagacacaaa
      901 ccatcgtcag ccaaatcacg caaaacctga tggaaggaat gttgatgaat ccgaccattg
      961 attcggacag caacaagaaa aactataatc tttacatggg aaaccatcat acactatcag
     1021 ctgtggatgg cgattttcag gttgatgcca taaaaactaa gacgcagttg gcagaggcac
     1081 aattgaagag atttagttat gagctgaaaa atgccttgcc ggatgcggca gccatccatt
     1141 acgccgtctg caaggattcg tcgggtaacg cgccgacatt ctccggcaat gctttttctt
     1201 caaattgcga caataaggca aacggggata ctttaattaa agtattgtgg gtaaatgatt
     1261 cggcagggga ttcggatatt tcccgtacga atcttgaggt gagcggcgac aatatcgtat
     1321 atacttatca ggcaagggtc ggaggtcggg aatgagacgt aaaatgctaa acgtaccaaa
     1381 aggcagttat gatggtatga agggttttac cattattgaa tttttggttg cgggcatgct
     1441 cagtatgatt gtcctgatgg cggtcggatc gagttacttc acatcccgga aattaaatga
     1501 tgcggcaaac gagcgtcttt ccgcacaaca ggatttgcgg aatgcggcaa cattgattgt
     1561 ccgcgatgcg agaatggcgg gcggcttcgg ttgtttcaat atgtccgagc atcctgcaac
     1621 tgatgttatt tccgatacga cgcaacaaaa ttctcctttt tccttaaaaa ggaacggtat
     1681 agataaactt atccccataa cggaatcttt aaatatcaat tatcagaatt ttttccaggt
     1741 tgatagcgca ttgatttttc aatacggaat cgatgatctt gatgcgagtg ctgagactgt
     1801 tgtagtcagc agctgttcca aaatagcaaa accgggtaag aaaatatcta ccttgcaaga
     1861 agcaaagagt gcattacaga ttactaatga tgataaacaa aatggaaata tcacccgtca
     1921 aaggcatgtg gtcaatgcct atgcggtcgg caggattgcc ggtgaggaag gtttgttccg
     1981 cttccaattg gatgataagg gcaagtgggg taatcctcag ttgctcgcga aaaagattag
     2041 acatatgaaa gtgcggtata tctatgtttc cgactgtcct gaagatgacg atgccggcaa
     2101 agaggaaaca ttcaaatata cggatacatt caacagcgcc aaagatgctg ttacgcccgc
     2161 cggggtggag gttttattga gtagcggtac tgataccaag attgccgctt cttcagacaa
     2221 tcatatttat gcttaccgta tcgatgcgac aatacgcggg ggaaatgtat gcgcaaacag
     2281 aacactttga cgggaatccc gacttctgac ggacagaggg ggtttgcact gtttatcgtg
     2341 ctgatggtga tgatcgtcgt ggcttttttg gttgtaactg ccgcgcagtc ttacaatacc
     2401 gagcagcgga tcagtgccaa cgaatcagac aggaaattgg ctttgtcttt ggccgaggcg
     2461 gctttgcggg aaggcgaact tcaggttttg gatttggaat atgatacgga cagtaaggtt
     2521 acatttagcg aagactgtgg aaaaggtctg tgtaccgcag tgaatgtgcg gacaaataat
     2581 gataatgaag aggcttttga caatatcgtg gtgcaaggca agcccaccgt tgaggcggtg
     2641 aagcgttctt gcactgcaaa ttctaccaac ctgtgcattg acaataaagg gacggaatat
     2701 aagaaaggca cgcaaagcgt cagcaaaatg ccacgttata ttatcgaata tttgggcgtg
     2761 aagaacggag aaaatgttta tcgggttact gccaaggctt ggggtaagaa tgccaatacc
     2821 gtggtcgtcc ttcaatctta tgtaagcaat aatgatgagt aataaaatgg aacaaaaagg
     2881 gtttacattg attgagatga tgatagtcgt cgcgatactc ggcatcatca gcgtcattgc
     2941 cataccttct tatcaaagtt atattgaaaa aggctatcag tcccagcttt atacggagat
     3001 ggtcggtatc aacaatattt ccaaacagtt tattttgaaa aatcccctgg acgataatca
     3061 gaacatcaag agcaaactgg aaatatttgt ctcaggctat aagatgaatc cgaaaattgc
     3121 cgaaaaatat aatgtttcgg tgcattttgt caataaggaa aaaccaaggg catacagctt
     3181 ggtcggcgtt ccaaagacgg ggacgggtta tactttgtcg gtatggatga acagcgtggg
     3241 cgacggatac aaatgccgtg atgccgcttc tgcccgagcc catttgaaga ccttgtccgc
     3301 agatgccggc tgtgaagcct tctctaatcg taaaaaataa ggttgttttg ccgatgccgt
     3361 ctgaaaatca atgttcagac ggtattttta tgggtatagt ggattaacaa aaatcgggac
     3421 aaggcgacga agccgcagac agtacaga
//
LOCUS       DAXHRI010000173         3426 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXHRI010000173 DAXHRI010000000
VERSION     DAXHRI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.173"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(56..565)
                     /locus_tag="ACFM75_001888"
     CDS             complement(56..565)
                     /locus_tag="ACFM75_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC6256379.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGATQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(898..1230)
                     /locus_tag="ACFM75_001889"
     CDS             complement(898..1230)
                     /locus_tag="ACFM75_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256380.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            complement(1275..1382)
                     /locus_tag="ACFM75_001890"
                     /pseudo
     CDS             complement(1275..1382)
                     /locus_tag="ACFM75_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1521..2357
                     /gene="cysT"
                     /locus_tag="ACFM75_001891"
     CDS             1521..2357
                     /gene="cysT"
                     /locus_tag="ACFM75_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC6256381.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     SGFWNTITEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGSGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            2612..>3426
                     /gene="cysW"
                     /locus_tag="ACFM75_001892"
     CDS             2612..>3426
                     /gene="cysW"
                     /locus_tag="ACFM75_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC6256382.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLQSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIVLATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGCQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIIT"
BASE COUNT          776 a          882 c          846 g          922 t
ORIGIN      
        1 aggcgaggca acgccgtact ggtttttgtt aatccgctat aattgatgaa acgggttaaa
       61 aaagtgttgc catcgcctgt tcttcttgtc ggatacgctt aaataagacc agcaaataaa
      121 tgggcaggcc aatcaataaa acataccacg cttgacataa taaggcgatg ccaatcagtt
      181 cgggtatgat gtttaaaaaa taattggggt ggcggaatgt tttaaacaac cacgaacgat
      241 taatttgatg atttggtaaa atatagattt taaccgtcca aatctccccc aactgcttaa
      301 taatcaatga caatatcaca aatgaagcca tcaccgtcag cgtaccaatc aaggatatgc
      361 cattaaaagc agtgtcagaa agccataccc aaacaaaaca cgccaaataa taaagcgtat
      421 gaactgccgc aagcactgtg gaattggttt ttccgtattg cgttgcccct ttggcaatca
      481 aggctttttc atgtttaata gagacggcta aaaataacag tctgatgata aaaaacaggc
      541 ttaaaatgct taaaatcatt gtcattgatg tttttccatt gaaattgaaa taaatataaa
      601 tcggattaat ggtattttta attaatgatg tttcagacca tcatgctcta taaacaattc
      661 ccattaagtc cgcgccgcaa cctgctataa taagtctgca atcggcgcaa atcaatgctt
      721 tgcgtttatt gccatcccaa aataattgat gatgccttaa ttataatacc aagataagtt
      781 ttttattcaa taaaatacaa agggaagcgt tcagcccatt gcaaacagat gcaatccacc
      841 gattatttaa aaaacggcaa agccttgccc cccccttgcg gcaagcctgc aatgccttta
      901 atgtccgcag gcgcaagcgt cgccgtggtg cccgcaaccc tgtgccgttt cgagccattc
      961 gtcgtcatcg ccgtcaaact cttccacttc gtcaaactcg ccgcgttcca ccatgacgca
     1021 caactcttca aggctttccg cgccttccac ccaaaaacag ggaatgcccg gcggcgtatc
     1081 gggcgcaagc agtgccgcta cgccttcatg ccacgcaatc caatagccgt tgtccgtacg
     1141 gacgaattgt gcgttttcgt tgaccgactc gccttccgta ccttccagca tacgttccgc
     1201 aatgtccggc tgaaaagata aaatctgcat aagtgttcct ttatatgatg gttttccgtc
     1261 aaaacaaggc gttatagtgg attaaattta aaccagtacg gcgttgcctc gccttgccgt
     1321 actatttgta ctgtctgcgg cttcgtcgcc ttatcctgat ttaaatttaa tccactatat
     1381 tttaaaacat cgcgcccgct tgagaaactg ccaaccgctt tataacaaat tcgtctttgc
     1441 accaaacttt ccattctttc cgtttttcgg acggcatcgt taaagtagtc cttccttttc
     1501 cttattttca gcattgtttt atgttagcgc tcaaaacgcc cggcgtactg cccggcttca
     1561 aactcagcct tggtctgacc gtattgtgcc tgtcgctgct tgtggtcttg ccgtttgcga
     1621 tgatggcggc gaaggcggcg gaaatcggct ggagcggatt ttggaacacg attaccgaac
     1681 ccaatgtctt ggcggccgtc tggctgagtt tgcggatgtc gttttatgcg atgctgacca
     1741 atgtcgtgtt cggcacgctg gtggcgtggg tattggtgcg ttatgaattc ccgggcaagg
     1801 gtctggcgaa cgcgctggtc gatttgccgt ttgcgctgcc gacggcggtt acgggtatcg
     1861 cgttggcgac cctgtatgcg cccaacggtt ggataggccg ttttttcgag cctttgggca
     1921 tcaaaatcgc gtttacgccc gtcggcattt ggattgcgct ggtcgtcgtc agcctgccct
     1981 ttatcgtccg cgccgtgcag ccggtgttgg aggaattatc gggcgaatat gaggaagcgg
     2041 cggcgacttt gggcgcaaac cgctttacga cgttccgccg cgtgctgctg ccggaaatca
     2101 cgccggcact cttgaccggc tcgggaatga tgttcgcgcg ggcaacgggg gaatacggct
     2161 cggtgatttt tatcgcgggc aatattccga tggtttctga aatcctgccg ctgattatta
     2221 cgggcaagct ggaacagttc gacgtgcagg gcgcgtcggc ggtggcgttg tttatgctgc
     2281 tggtttcgtt tgtgattctg tttgcgctga acgtgatgca gtgggcgttg ggcaggcgtt
     2341 cgggcgcgaa gggttgaggt cgtctgaaat acctgttacc gtcattcccg cgcaggcggg
     2401 aatccattgg tgaatttcgg ctgccttatt tattttctgt tttcctgttg ccctgcggtg
     2461 gattcccgcc tgcgcgggaa tgacggtagc tagacgtttt tattccctta atcaataaaa
     2521 ggttgtctga aaacgaatcc gccccacaaa aaacggtttt tcagacggca tccgaacacc
     2581 ttaaaacaac caaccgggga acaccaccgc tatgaaaccc tattccgcca atcccaacct
     2641 gaccgaaccg cgccggctgc gcgttttgct gattgccgcc gcgctgggct ttctgctgct
     2701 gatgctggtc gtgccgctcg tcgccgtgtt ttacgaagcc ttaaaaggcg gttgggattt
     2761 gtacctgcaa tccttaaacg atcccgaagc gtggtctgcc atcaaattga cgctgattac
     2821 cgcgctgatt gtcgttcccg tcaatgccgt cttgggcgta gcgatggcgt ggctgctgac
     2881 ccgttttgat tttcgcggca agcagttgct gaccaccctg ctcgatttgc cgttttccgt
     2941 atcgcccgtg gtggccggtt tgatgttcgt cttattgttc ggtgcgcaca cggcattggg
     3001 tggctggctc gaagcgcaag gcatacagat tatcttcgcc atccccggta ttgttttggc
     3061 gacgctgttc gttactttcc cctttgtcgc acgcgaaatc atcccgctga tgcaggctca
     3121 gggcgacagc gaagaacagg cggcattgat actcggcgca agcggctgtc agatgttttg
     3181 gcgcgttacc ctgcccaaca tcaaatgggc gttactctac ggcatcatcc tcaccaacgc
     3241 ccgcgcgatg ggcgagttcg gcgcggtcag cgtggtatcg ggacacatac gcggcgaaac
     3301 caacaccgtc ccgcttttgg tcgaaatctt ctacaacgaa tacaacttta ccggcgcatt
     3361 cgccctctcc ggcgtattgg cacttttagc actggcgacg ctggcggtgc agaacatcat
     3421 caccaa
//
LOCUS       DAXHRI010000174         3381 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXHRI010000174 DAXHRI010000000
VERSION     DAXHRI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.174"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     regulatory      65..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            234..1457
                     /gene="cytX"
                     /locus_tag="ACFM75_001893"
     CDS             234..1457
                     /gene="cytX"
                     /locus_tag="ACFM75_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC6256383.1"
                     /translation="MSGNASSLSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            1454..2554
                     /locus_tag="ACFM75_001894"
     CDS             1454..2554
                     /locus_tag="ACFM75_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC6256384.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWHADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            2575..3192
                     /gene="thiE"
                     /locus_tag="ACFM75_001895"
     CDS             2575..3192
                     /gene="thiE"
                     /locus_tag="ACFM75_001895"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC6256385.1"
                     /translation="MTFLPLKSPLKFYAVVPTADWVERMVEAGADTVQLRCKALHGDE
                     LKREIARCAAACRGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLEKLREYVKQAGSTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            3180..>3381
                     /locus_tag="ACFM75_001896"
     CDS             3180..>3381
                     /locus_tag="ACFM75_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256386.1"
                     /translation="MGWIKPKEENSIAVSAKLSPLSQTYLTLNVAYHQIPSFPRKRES
                     ALKLEKPSFEKQFPNFKNGFPPA"
BASE COUNT          675 a         1066 c          934 g          706 t
ORIGIN      
        1 aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg ccccctgctt ttgtgtacac
       61 ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt
      121 agaacctgtc ggggtaatgc ctgcgtagga aacaaaacct ccccgccctt tcgggcttcc
      181 gttccctttt ccgcacttcc ccgccccatt ttcatgtttt ttaaggactt gatatgtcgg
      241 gcaatgcctc ctctctttca tcttcctccg ccatcgggct gatttggttc ggcgcggcgg
      301 tatcgattgc cgaaatcagc acgggtacgc tgcttgcgcc tttgggctgg cagcgcggtc
      361 tggcggctct acttttgggt catgccgtcg gcggcgcgct gttttttgcg gcggcgtata
      421 tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg cctgtcgttc ggcaaacgcg
      481 gttcagtgct gttttccgtg gcgaatatgc tgcaactggc cggctggacg gcggtgatga
      541 tttacgccgg cgcaacggtc agctccgctt tgggcaaagt gttgtgggac ggcgaatctt
      601 ttgtctggtg ggcattggca aacggcgcgc tgattgtgct gtggctggtt ttcggcgcac
      661 gcaaaacagg cgggctgaaa accgtttcga tgctgctgat gctgttggcg gttctgtggc
      721 tgagtgccga agtcttttcc acggcaggca gcaccgccgc acaggtttca gacggcatga
      781 gcttcggaac ggcagtcgaa ttgtccgccg tcatgccgct ttcctggctg ccgctggccg
      841 ccgactacac acgccaagca cgccgcccgt ttgcggcaac cctgacggca acgctcgcct
      901 atacgctgac gggctgctgg atgtatgcct tgggtttggc agcggcgttg ttcaccggag
      961 aaaccgacgt ggcaaaaatc ctgctgggcg caggtttggg tgcggcaggc attttggcgg
     1021 tcgtcctctc caccgttacc accacttttc tcgatgccta ctccgccggc gtaagtgcga
     1081 acaacatttc cgcgcgtttt gcggaaacac ccgtcgctgt cggcgttacc ctgatcggca
     1141 cggtgcttgc cgtcatgctg cccgttaccg aatatgaaaa cttcctactg cttatcggct
     1201 cggtatttgc gccgatggcg gcggttttga ttgccgactt tttcgtcttg aaacggcgtg
     1261 aggagattga aggctttgac tttgccggac tggttctgtg gcttgcgggc ttcatcctct
     1321 accgcttcct gctctcgtcc ggctgggaaa gcagcatcgg tctgaccgcc cccgtaatgt
     1381 ctgccgttgc cattgccacc gtatcggtac gccttttctt taaaaaaacc caatctttac
     1441 aaaggaaccc gtcatgaccc gtatcgccat cctcggcggc ggcctctcgg gaaggctgac
     1501 cgcactgcag cttgcagaac aaggttatca gattgcactt ttcgataaag gctgccgccg
     1561 gggcgaacac gccgccgcct atgttgccgc cgccatgctc gcgcctgcgg cggaagcggt
     1621 cgaagccacg cccgaagtgg tcaggctggg caggcagagc atcccgcttt ggcgcggcat
     1681 ccgatgccgt ctgaacacgc acacgatgat gcaggaaaac ggcagcctga ttgtgtggca
     1741 cgggcaggac aagccattat ccagcgagtt cgtccgccat ctcaaacgcg gcggcgtagc
     1801 ggatgacgaa atcgtccgtt ggcacgccga cgacatcgcc gaacgcgaac cgcaactcgg
     1861 cggacgtttt tcagacggca tctacctgcc gaccgaaggc cagctcgacg ggcggcaaat
     1921 attgtctgca cttgccgacg ctttggacga actgaacgtc ccctgccatt gggaacacga
     1981 atgcgtcccc gaagacctgc aagcccaata cgactggctg atcgactgcc gcggctacgg
     2041 cgcaaaaacc gcgtggaatg cttctgccga acacggcagc accctgcgcg gcatacgcgg
     2101 cgaagtggcg cgggtttaca cacccgaaat cacgctcaac cgccccgtgc gcctgctgca
     2161 cccgcgttat ccgctctaca tcgccccgaa agaaaaccac gtcttcgtca tcggcgcgac
     2221 ccaaatcgaa agcgaaagcc aagcccccgc cagcgtgcgt tcagggttgg aactcttgtc
     2281 cgcactctat gccgtccacc ccgccttcgg cgaagccgac atcctcgaaa tcgccaccgg
     2341 cctgcgcccc acgctcaacc accacaaccc cgaaatccgc tacagccgca aacgccgcct
     2401 catcgaaatc aacggccttt tccgccacgg tttcatgatc tcccccgccg taaccgccgc
     2461 cgccgtcaga ttggcagtgg cactgtttga cggaaaagat gcacccgaac gcgatgaaga
     2521 aagcggtttg gcgtatatcc gaagacaaga ttaaagccgt ctgaaaggaa ccccatgacc
     2581 ttcctgccac tcaaatcccc gctcaaattc tacgccgtcg tccccaccgc cgattgggtc
     2641 gagcgcatgg tcgaagcagg tgccgacacg gtgcaactgc gctgcaaggc cctgcacggc
     2701 gatgaattga aacgcgaaat cgcccgctgc gccgcagcct gccgtggcag ccgtacgcag
     2761 cttttcatca acgaccactg gcgcgaggca atcgaagcag gcgcgtacgg cgtgcatctc
     2821 ggacaagaag acatggacac cgccgacctc gccgccattg ccgctgccgg tttgcgtttg
     2881 ggtttgagta cgcactccgt cgccgaactc gaccgcgccc tgtccgtgca ccccagctac
     2941 gtcgccagcg gcgcgatttt cccgaccacg accaaacaaa tgcccaccgc ccctcaaggc
     3001 ttggaaaaac tgcgtgaata tgtgaaacag gcaggcagca cgcccgtcgt cgccattggc
     3061 ggcatcgatt tgaacaacgc ccgagccgta ctcgccaccg gcgtttcctc actcgccgcc
     3121 gtccgcgccg taaccgaagc ggcaaatccc gaagcggtgg ttaaagcgtt tcaggctttg
     3181 tgggatggat aaaaccgaaa gaagaaaatt caattgccgt gtcggcaaaa cttagtccgt
     3241 tatcgcaaac atacttaact ttaaatgtgg catatcatca aattccgtca ttcccgcgta
     3301 agcgggaatc cgccttaaaa cttgagaaac catcatttga aaaacagttt ccgaatttca
     3361 aaaatggatt cccgcctgcg c
//
LOCUS       DAXHRI010000175         3349 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXHRI010000175 DAXHRI010000000
VERSION     DAXHRI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.175"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(205..1023)
                     /gene="cysE"
                     /locus_tag="ACFM75_001897"
     CDS             complement(205..1023)
                     /gene="cysE"
                     /locus_tag="ACFM75_001897"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC6256387.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            1207..1785
                     /gene="grpE"
                     /locus_tag="ACFM75_001898"
     CDS             1207..1785
                     /gene="grpE"
                     /locus_tag="ACFM75_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC6256388.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(1809..1949)
                     /locus_tag="ACFM75_001899"
     CDS             complement(1809..1949)
                     /locus_tag="ACFM75_001899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256389.1"
                     /translation="MKKNRQAAYGLTVNTETKCRLKPDRRVQTALPLYFDRFIRKTRF
                     RK"
     gene            complement(1909..2124)
                     /gene="nqrM"
                     /locus_tag="ACFM75_001900"
     CDS             complement(1909..2124)
                     /gene="nqrM"
                     /locus_tag="ACFM75_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC6256390.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(2150..3205)
                     /locus_tag="ACFM75_001901"
     CDS             complement(2150..3205)
                     /locus_tag="ACFM75_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6256391.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAYVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
BASE COUNT          734 a          756 c         1007 g          852 t
ORIGIN      
        1 cgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tatttccggt
       61 tgcgtgggaa tcgggtgtat cgaataaaag gcattttgtc cgactggcaa gtgccgacat
      121 cggcggcata tcaaggcgca ggcttgaagc gggcaatgtc gtctgaagcc cgtttggcgt
      181 ttcagacggc attggtgcgg atattcaaat cataaagtcg atttcggcaa actggatatt
      241 ttgatccata tccgccgacg gtgttttgag cgatcgcgcc acgggtttgg cgggtacgcc
      301 gacaaccgtg atggacggcg gcacgtctga aaccacgacg ctgcccgccc cgattttggc
      361 attgctgccg atgcggatat tgcccaatat cgaggcgttt gcgccgatca tcacgccgtc
      421 gccgatttta gggtggcggt cgccgccttc tttgcccgaa ccgccgagcg ttacgccgtg
      481 caaaatcgaa atattgttgc ccaacacggc ggtttcgccg gcaacaaagc cggtggcgtg
      541 gtcgagcatc agcccgtatc cgaaacgggc ggcgggatgg atgtccacgc cgaatacttc
      601 ggacatacgg ttttgcagga aatacgccag cgttttgcgc ccgtcgagat acagccggtg
      661 gttgatgcgg tgtgcctgaa tcgcgtggaa gcctttgaaa tataaaagcg gcagcgaata
      721 ttcgtcgcag gcgggatcgc gttcgtagat ggcttttaag tctgcttcga cgcatttgcc
      781 gatttgggtg tcgctgccca acgcctgctg gtagatttca aacagcgcgc gcacgtccat
      841 aatcgggctg ccgagtttgc tggaaaggtg gtaggcaagg acggagccga gggactcgtg
      901 gcgcaacacg gtttggtgca aaaaacttgc cagcatcggt tcggcggaga ccgcggccgc
      961 ggtttcttcg cggatggtgt gccagaggtc gaaaccggtt gtgtttaaat ggtctttttt
     1021 catgagtgat gacgtttgaa aatcgatatg gtcggcagta tcttaccgtc tatattattt
     1081 tttcggtagg ggatttgaaa atgaatttga aattctctgc ttttgcttga agtttcttga
     1141 aaatgtcctt atcttgcgcg ggtaataact ggattttgat ttccaatttg ttttaaggga
     1201 tacgatatga gcgaacagac acagcagcaa aacagtgaag aagcggttga aaatgtggag
     1261 gcggtggaaa ccgtcgagac agtaggaaat gcggacggtg tgcaggaaca ggctgccgca
     1321 gagccggctt atgaggattt gcaggcgcgg attgccgagc tggaagcgca gttgaaagac
     1381 gagcagctgc gcgctttggc aaacgagcaa aacctgcgcc gccgccacca gcaggaaatt
     1441 gcggatacgc acaagttcgc cggacagaag tttgccgtgg aaatgctgcc ggtcaaggat
     1501 tatctggaaa tggcgctttt ggatcagagc ggcaatttcg atgcgctgaa aatgggcgtg
     1561 cagatgactt tgaacgagtt gcagaaagca tttgatgcta cgcaaatcaa ggaaatcaac
     1621 cctaaagcgg gcgataagct cgatccgaat atccatcagg cgatgcaggc ggtggcaagc
     1681 gaacaggagc cgaataccgt ggtgggtgtg atgaagaagg gttatacgct gtccgaccgc
     1741 gtgttgcgcc cggctatggt tacggtggca cagaaggaag cctgaaggcg tctggggaat
     1801 aatctgattt atttcctgaa gcgcgttttg cgtataaacc gatcgaagta aagcggcaat
     1861 gccgtctgaa cccgcctgtc gggcttcaga cggcattttg tttcggtgtt aacggtcaac
     1921 ccgtatgccg cctgcctgtt tttcttcatc cagtttcttt tgcagggtgt cgcaaggtgt
     1981 gtcgcagtcg cacatttttt tcatacccaa ggcagtaatg ccgccgcaac tgcctttgat
     2041 gctgcgtttg gagaaaatat agccgaccgc cataccgatg atgacggtca ggaagatgcc
     2101 gaaggtaagg agcagggttt tcatggtgtt tcctaatcgg tttgtatgtt tagcggagca
     2161 gtttttcaaa ttcggaagac atggcggtgc ggtagccgcc tttatccctg acaatcagga
     2221 aaacagcgag tttttcgcgc tctgccagct ttaaggcttc ggtttcgccc aatacgaata
     2281 atcctgtgga caagccgtca gccgtcatcg cactgtctgc gaccacgctg atggaggcga
     2341 ggttgtggct gatgggtcgt ttgttgttcg ggttgatgat atgggagagg cgtttgccgt
     2401 ttttatcgac gtggaaaata cggtaatcgc cggaagtggc aagcgaacgg ttgttcagcg
     2461 ggacgataat ctgcgtattg ccgccttgga cgatattggg ctgctcgata ccgatgcgcc
     2521 acggttcgcc gcgcgcgttt ttgcctttgc cgtgcaactc gccgccgatt tcgaccagat
     2581 aattttgaat gccgtatttt tccagttcgc ccgcaacttt atcaacgccg aagcctttgg
     2641 caatcgaaga taaatccaaa taggccttgg ggtgggtttt gctcaaggaa gcgtaatctt
     2701 tgccttgttt caaaatgatt ttgtctatgc ccgtataaga tgccgcctgt ttgatttgtt
     2761 ccggcgacgg ttcacgggta acggatttgt cggggccgaa tccccaaagg ttgaccaaag
     2821 ggccgacggt tacgtccagc gcgccgtgtg tcaggcggtt taggcggacg gcttcggcag
     2881 taacgtatgc gaagtcgctt gaaatgcgga ggggcttgcc ggctgtgtgt tggttgaacc
     2941 ggctgatttc ggagtcgggc tgataggtgg acatctgccg gttgacttct ttaagcgcgt
     3001 catcgatgcg cttttgtatt tcggcaggtg aggggagttt gtcccgatta tttgaaaggt
     3061 atttgacggt ataggtcgtg cccatcgttt cgccttgcag ggtaacggtt tgcgcggttt
     3121 gttccgaaca ggcgttcagg aagatgaaac ccagggcaaa tatcaagacg cggataaagt
     3181 tcggcaggcg tgtttcagac ggcatagtgt ttgacggttt tggcaaatgg tttgaattat
     3241 atcgcaaaac ggccgatatg tttctatgcc gatgccgtct gaagggcatc aggggcatcg
     3301 cccgctgttt gggggcggta ataaaaaaac aaaccggtgt tttacaaac
//
LOCUS       DAXHRI010000176         3343 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXHRI010000176 DAXHRI010000000
VERSION     DAXHRI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.176"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            73..702
                     /locus_tag="ACFM75_001902"
     CDS             73..702
                     /locus_tag="ACFM75_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC6256392.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            877..3242
                     /gene="hmbR"
                     /locus_tag="ACFM75_001903"
                     /pseudo
     CDS             877..3242
                     /gene="hmbR"
                     /locus_tag="ACFM75_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
BASE COUNT          902 a          880 c          822 g          739 t
ORIGIN      
        1 ccactatata tgcgggcaag tgcggatttt gcccgccccg catccttatc ccttaccgac
       61 aaaggaaaac ttatgagtga aaccgaaaat caagccctga cgtttgccaa acgtctgaag
      121 gcggatacca cggcggttca cgacagcgtg gataacctcg ttatgtctgt ccaaccgttt
      181 gtcagcaaag aaaactacat caaatttttg aaactccaat ccgttttcca caaggccgtc
      241 gaccacatct ataaagatgc cgaattaaac aaagccattc ccgagctgga atacatggcg
      301 cgatacgatg ccgtaacgca agaccttgca gatttgggcg aagagcctta caaattcgac
      361 aaagaattac cgcatgaaac cggcaacaaa gcaatcggct ggctttattg cgccgaagga
      421 tccaatttgg gcgcggcgtt tttgttcaaa cacgcccaaa aactcgatta caacggcgaa
      481 cacggcgcgc gccacctcgc tccgcatccc gacgggcgcg gcaaacactg gcgcgccttc
      541 gtcgagcatc tgaacgcttt gaacctgact cccgaagccg aagcggaagc cattcaaggc
      601 gcgcgcgaag cctttgcatt ctacaaagtc gtgttgcgcg aaaccttcgg cttggcagcc
      661 gatgccgaag cccccgaagg tatgatgccg cacaggcact aaaaaataat cgaaccaaat
      721 aaacaaggtc tcggcatagc tgtttgcagg gacctttaat tacacggcgc ggctttgttt
      781 acatggatta ctgtcttatt aaatattaat gattatcata aaatctatta ttcgctaacc
      841 gatggatgaa caatccatac atcttgagtt gataatatga aaccattaca aatgctccct
      901 atcgccgcgc tggtcggcag tattttcggc aatccggtct ttgcggcaga tgaagctgca
      961 actgaaacca cacccgttaa ggcagaggta aaagcagtgc gcgttaaagg ccagcgcaat
     1021 gcgcctgcgg ctgtggaacg cgtcaacctt aaccgtatca aacaagaaat gatacgcgac
     1081 aacaaagact tggtgcgcta ttccaccgat gtcggcttga gcgacagcgg ccgccatcaa
     1141 aaaggctttg ctgttcgcgg cgtggaaggc aaccgtgtcg gcgtgagcat agacggcgta
     1201 aacctgcctg attccgaaga aaactcgctg tacgcccgtt atggcaactt caacagctcg
     1261 cgtttgtcta tcgaccccga actcgtgcgc aacatcgaca tcgtaaaagg ggcggactct
     1321 ttcaataccg gcagcggcgc cttgggcggc ggtgtgaatt accaaaccct gcaaggacgt
     1381 gacttactgt tgcctgaacg gcagttcggc gtgatgatga aaaacggtta cagcacgcgt
     1441 aaccgtgaat ggacaaatac cctcggtttc ggcgtgagca acgaccgcgt ggatgctgct
     1501 ttgctgtatt cgcaacgtcg cggtcatgaa accgaaagcg cgggaaaccg aggctatgct
     1561 gtggaagggg aaggcagtgg cgcgaatatc cgtggttcgg cacgcggtat ccctgattcg
     1621 tccaaacaca aataccacag ctttttgggt aagattgctt accaaattaa cgataaccac
     1681 cgcatcggcg catcgcttaa cggccagcag ggacataatt acacggttga agagtcttat
     1741 aacctgaccg cttcttcctg gcgcgaagcc gatgacgtaa acagacggcg caatgccaac
     1801 ctcttttacg aatggatgcc tgattcaaat tggttgtcgt ctttgaaggc ggatgtcgat
     1861 tatcaaaaaa ccaaagtatc tgcggtcaac tacaaaggtt cgttcccgat agaggattct
     1921 tccaccttga cacgtaacta caatcaaaag gacttggatg aaatctacaa ccgcagtatg
     1981 gatacccgct tcaaacgcat taccctgcgt ttggacagcc atccgttgca actcgggggg
     2041 ggcgacaccg cctgtcgttt aaaactttcg ccagccgccg tgattttgaa aacctaaacc
     2101 gcgacgatta ttacttcagc ggccgtattt tacgcacgtc cagcacgatt cagcatccag
     2161 tgaaaaccaa caactacggt ttctcgctgt ctgaccaaat ccaatggaac gacgtgttca
     2221 gcagccgcgc aggtatccgt tacgactaca ccaaaatgac gccgcagcaa ctgaatgccg
     2281 agtgtcatgc ttgtgacaaa acaccacctg cagccaacac ttataaaggc tggagcggtt
     2341 ttgtcggctt ggcggcgcaa ctgaatcagg cttggcgtgt cggttacgac attacttccg
     2401 gctaccgtgt ccccaatgcg tccgaagtgt atttcactta caaccacggt tcgggtaatt
     2461 ggctgcccaa tcccaacctg aaagccgagc gcagcaccac ccacaccctc tctctgcaag
     2521 gccgcagcga aaaaggtact ttggatgcca acctgtatca aagcaattac cgcaatttcc
     2581 tgtctgaaga gcagaagctg accaccagcg gcactcccgg ctgtactgag gaaaatgctt
     2641 actacggtat atgcagcgac ccctacaaag aaaaactgga ttggcagatg aaaaatatcg
     2701 acaaggccag aatccgcggt atcgagctga caggccgtct gaatgtggac aaagtagtgt
     2761 cttttgttcc tgagggctgg aaactgttcg gctcgctggg ttatgcgaaa agcaaactgt
     2821 cgggcgacaa cagcctgctg tccacacagc cgctgaaagt gattgccggt atcgactatg
     2881 aaagtccgag cgaaaaatgg ggcgtgttct cccgcctgac ctatctgggc gcgaaaaagg
     2941 tcaaagacgc gcaatatact gagtatgagg gctaccgatt tatcaaaaaa gtcaaagatt
     3001 atccgtggtt gaataagtcg gcttatgtgt tcgatatgta cggcttctac aaaccggtga
     3061 aaaacctgac cctgcgtgcg ggcgtgtaca acctgttcaa ccgcaaatac accacttggg
     3121 attccctgcg cggtttgtat agctacagca ccaccaatgc ggtcgaccgc gatggcaaag
     3181 gcttagaccg ctaccgcgcc ccaggccgca attacgccgt atcgctggaa tggaagtttt
     3241 aatctagtat tattggatta attgtctcgt tggaaattaa agccgtccga attgtgttca
     3301 agaactcatt cggacggctt tttaccgtaa atctgtgtga tgg
//
LOCUS       DAXHRI010000177         3318 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXHRI010000177 DAXHRI010000000
VERSION     DAXHRI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.177"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(181..360)
                     /locus_tag="ACFM75_001904"
     CDS             complement(181..360)
                     /locus_tag="ACFM75_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256393.1"
                     /translation="MPSERTFCRSDGIFISFFRLPPLENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            complement(376..1383)
                     /locus_tag="ACFM75_001905"
     CDS             complement(376..1383)
                     /locus_tag="ACFM75_001905"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC6256394.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGGDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            1615..1851
                     /gene="acpP"
                     /locus_tag="ACFM75_001906"
     CDS             1615..1851
                     /gene="acpP"
                     /locus_tag="ACFM75_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6256395.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            2007..3254
                     /gene="fabF"
                     /locus_tag="ACFM75_001907"
     CDS             2007..3254
                     /gene="fabF"
                     /locus_tag="ACFM75_001907"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC6256396.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPGIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSLRMIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT          788 a          911 c          872 g          747 t
ORIGIN      
        1 aaaatcccct aaatgtcttg gtgggaattt aggggatttt ggggaatttt gcaaaggtct
       61 caatcttttg attatccgcc gtatccttat tttccacatc atgaatttca acgccgttca
      121 tatttttcgt tttctcaaac cgtccaccac ggcataaaat tattttttca tatcaattat
      181 ttatatgttt aacaacttac cgccggactt gctttggctg cgtatcaaga ttgtccggtt
      241 cgcggcgggc gtttgctttg tttcaaacag ggcggcgcgt aagggatttt caaggggcgg
      301 caggcggaaa aaggaaatga aaatgccgtc tgaacggcaa aacgtgcgtt cagacggcat
      361 tttgggcggg acgcgtcatc gcgccaaagc cttcaaacat tctttgacca atgccggacc
      421 tttgtatatc aatccgctgt acacttggac ggcggtcgcg cccaagcgga ttttctctgc
      481 cgcgtccccg ccttccataa tgccgcctac gccgataatc ggcagcttgc cgtcgatgtg
      541 gtctgccaac agcttcaaca cccgattact tttttcatga acgggcagcc cgctcaaacc
      601 gccctgctcg cctgcgagcg gatggctgcc gagacttgat ttgtcgatgg tggtattggt
      661 agcgatgatg ccgtccattt cgacggattt gacaacgtgg gcgatgtctt cgatttgtgc
      721 ttcatccaaa tcgggggcga ttttgacggc gagcggaacg tatttcccgt atacagaggc
      781 aagctgtgcc tgtttgtttt tcaaagcctc aagcaatgcg ctcaactcgt cgccaccttg
      841 cagcgcgcgg aggtttttgg tgttgggcga ggaaatattg acggtaatgt agcttgcgtg
      901 tgcgtaggct ttttcaaggc agattaaata atcatcggca gcgttttcga tgggtgtaac
      961 cgcgttttta ccgatgttga tgcccaatac gccactgaat ttactttttt cgatgttgcg
     1021 tatcatggtg tcgataccgt ggttgttgaa acccatgcgg ttgatgatgc cttggtgttc
     1081 gggaacgcga aagaggcgcg gctgcgggtt gccgggctgc gggttgggcg ttaccgtgcc
     1141 gatttcgatg aagccgaaac cgagcgcgcc caatgcgtcg atgtattcgc cgtttttgtc
     1201 gagtccggcg gcaagcccga cagggttggg caaatccaaa cccatcagtt ttacaggttt
     1261 ggtacggttg ccggttacgg gaatcagacc caatttataa accatgttga gcgcgtcgag
     1321 cgtgaagtgg tgggctttct cggcatcgag tgcaaacagg atgcggcggg caagtggata
     1381 catgacaggg ctttctgctt cagatggaaa tgccgtcatt ttaaccgaaa tcccgtcaaa
     1441 catacggcac acaggtcaaa cttgaacgac aaaacccgcc gcacccaaaa atgtacaaat
     1501 cggcaaatat tgatataata gtcattagat agaaaactct attaaaagtc ggtacaatac
     1561 caccgattta tttcccttaa acaacctgcc gcaaggcata aaggaacgac tgatatgtca
     1621 aacatcgaac aacaagttaa aaaaattgtt gctgaacaac tgggcgtaaa cgaagccgac
     1681 gtgaaaaacg aatcttcttt ccaagacgac ttgggcgcgg attctttgga caccgtggag
     1741 ctggttatgg ctttggaaga agcattcggc tgcgaaatcc ccgacgaaga tgccgaaaaa
     1801 atcaccaccg tccaactggc tatcgactac atcaatgccc acaacggcta accggtcgtc
     1861 gcccgacaca acagcctctg ctgcaccgcg caatagaggc ttttccctta tatggcaaac
     1921 tgtttgaacc cctgccgcaa attacagacg gcatctgcac gacagccgca tatgccccca
     1981 ttccgacaac caacagcgag gttatcatga gtcagagaag agtagtcatt acaggcttag
     2041 gtcaggtttc ccctgtcggc aacactgtcg cagaggcttg ggacaccctg ctcgccggca
     2101 aaagcggcat cggcgcgatt acccgctttg acgcatccga catcaacagc cgtgtcgccg
     2161 gcgaggtgcg cggtttcgac atcggacaat acatcagcgc gaaagaagcg cgccggatgg
     2221 acgtattcat ccactacggc attgccgccg cattgcaggc aatcgccgat tcgggtttgg
     2281 acgatgtgga aaacctcgac aaagaccgca tcggcgtgaa catcggttcc ggcatcggcg
     2341 gactgcccgg catcgaggtc accggcaaag ccgtaatcga aggcggcgcg cgcaaaatca
     2401 accctttctt tatccccggt tctctgatta atctgatttc cggacacgtt accatcctca
     2461 aaggctaccg cggcccgagc tacgggatgg tttccgcctg caccaccggc gcgcacgcca
     2521 tcggcgattc cctccgtatg attaaatacg gcgacgcgga cataatggtt gccggcggcg
     2581 cggaaggcgc aatcagcact ttgggcgtgg gcggttttgc cgcgatgaaa gccctctcca
     2641 cccgcaacga cgaccccgcc accgcttccc gtccgtggga caaaggccgc gacggcttcg
     2701 ttatcggcga aggcgcgggc atattggtgt tggaagaatt ggaacacgcc aaaaaacgcg
     2761 gcgcgaaaat ctacgccgaa atcgtcggct tcggcatgag ttccgatgct taccatatca
     2821 ccgcgccgaa cgaagaaggc cccgcccttg ccgttacccg cgcgctgaaa gatgccggca
     2881 tcaatcccga agacgtggat tacgtcaacg cgcacggcac gtccaccccc ttgggcgatg
     2941 ccaacgaaac caaagccctc aaacgcgcgt tcggcgaaca cgcctacaaa accgtcgtca
     3001 gctcgaccaa atccatgacc ggccacctgc tcggcgcggc gggcggcgtg gaggccgtgt
     3061 acagcatttt ggcgatacac gacggcaaaa tcccgccgac catcaacatt tttgaacaag
     3121 acgttgaagc cggctgcgat ttggactact gcgccaacga agcgcgcgac gcggaaatcg
     3181 acgttgccat ttccaactcc ttcggcttcg gcggcaccaa cggcacgctg gtcttcaaac
     3241 gcttcaaagg ctgattccgc aaagccgccg ccgacatcga aatgccgtct gaaaccgttt
     3301 cagacggcat ttttatag
//
LOCUS       DAXHRI010000178         3242 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXHRI010000178 DAXHRI010000000
VERSION     DAXHRI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.178"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(205..1055)
                     /locus_tag="ACFM75_001908"
                     /pseudo
     CDS             complement(205..1055)
                     /locus_tag="ACFM75_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            1405..2919
                     /gene="katA"
                     /locus_tag="ACFM75_001909"
     CDS             1405..2919
                     /gene="katA"
                     /locus_tag="ACFM75_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC6256397.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2995..3216
                     /locus_tag="ACFM75_001910"
     CDS             2995..3216
                     /locus_tag="ACFM75_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256398.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFPRHSRESGNLERKI"
BASE COUNT          786 a          915 c          826 g          715 t
ORIGIN      
        1 aaacccccgt cattcccgcg aaagcgggaa tccaggttcg ttgagtttcg gtcatttccg
       61 ataaattctt gcagctttgc gtttctagat tccctctttc gcgggaatga cgggatgcag
      121 gtttccgcgc ggacggattc ggcattcccg ctttcgcaga aatgccggct tttggtttgt
      181 tgtctttata agattattcg gcaattattc tgcggttttg cgtttgtgtg ctgccggaat
      241 ctcaagggat tctctgtatt tggcaatggt gcggcgagaa acctccatgc cgcggaaagc
      301 cagcaggttg gcgagtgcct cgtcagaata cggcgtgggt gtggacgagg caaaaccggc
      361 ggcgggaaag ggattgagcg aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc
      421 ggtttggggc aggtgttttt tattcgtgcg agggtttggc tgcggttgcc gtcaatgctg
      481 tcgagggcgt ttcggacgat atgcagggcg gcgggtttgg cagcacattc gcccaatctt
      541 tctatctgca gtatcagcga ttcgttcaaa tcggcggcgg ccacgcctgc cgggtcgaat
      601 tttttcaatg cggtcagcgc gtgttgcagc attgcttcat ccaacatcca ctctaagggc
      661 gtatggtcga ggatgtcttc gatgctgtcg gtcagataac cctgctcgtc aaggaaatcg
      721 ataaggatgt ggacacaggc ggcttcttgg tcggaaagcg ggtgttcgca tacttgcgcg
      781 tgcaggtatt gcttgaaatc ctgctcaccg gcgatgttgg acagcatatc ttcgccttcg
      841 tctccgccga tttgacgggc aggcgcggtg taatgggaaa acctagcatc ggaaaattca
      901 tccgtgtctt tgcgttcgag caggggattg tccgacagcc agttttcgac ctcgcgttca
      961 agttcgatac ccgacatctg caatacgcgc aaagattgtt gcagccgctg gttgagctgc
     1021 tgggtctgtt tgagctttat tccgattaag gtcatgataa tgtgggggaa attgttattt
     1081 tcagcctgtc ggcgcaaaaa aaatgccgca aagcgtcatt gcattataaa tggttttaat
     1141 gagcgggttc ggattctttc tgataacaaa aaacaaacga aaatcaaaaa ccgattgctt
     1201 ataacaatat taaatcgatt tcatagtttt aatagcgaaa atcttggcgt atagtcgcat
     1261 ccatagtttt tacaaaagga aaacaaaatg tcgattcaag aaatttattg caatcaagaa
     1321 accggttacg aatacgcttt ccgccaaatc gtactgtaag ccgtttaccc ccatttgacc
     1381 aacctgagaa aaggaacaag agcgatgact acctccaaat gccccgtcac ccatctgacc
     1441 atgaacaacg gcgcgcctgt tgccgacaat caaaacagcc tgaccgccgg tccgcgcggc
     1501 cccctgctgg cgcaggattt gtggctgaat gaaaaactcg ccgacttcgt gcgcgaagtc
     1561 attcccgaac gccgtatgca cgccaaaggt tcgggcgcgt tcggtacgtt taccgtaaca
     1621 cacgacatca ccaaatacac ccgcgccaaa atcttcagcg aagtcggcaa aaaaaccgag
     1681 atgttcgccc gtttcaccac cgtggcaggc gaacgcggcg cagccgatgc cgaacgcgac
     1741 atccgcggct ttgccttgaa attctatacc gaagaaggca actgggatat ggtcggcaac
     1801 aacacgcccg tgttcttcct gcgcgatccg cgtaagttcc ctgacctgaa caaagccgtc
     1861 aaacgcgatc cgcgcaccaa tatgcgctct gccacaaaca actgggactt ctggacgctg
     1921 ctgcccgaag cactgcacca agttaccatc gtgatgagcg accgcggcat ccccgccggc
     1981 taccgccata tgcacggctt cggttcgcat acctacagct tctggaacga agcaggcgag
     2041 cgtttttggg tgaaattcca tttccgcacc caacaaggca ttaaaaacct gaccaacgaa
     2101 gaagccgcca aaatcatcgc cgacgaccgc gaaagccatc agcgcgactt atacgaagcc
     2161 atcgaacgcg gcgagtttcc gaaatggacg atgtacatcc aagtcatgcc tgaagcagac
     2221 gcggaaaaag taccttatca tccgtttgac ttgaccaaag tttggccgaa aaaagactat
     2281 ccgctgattg aagtgggcga attcgagttg aaccgcaatc ccgaaaactt cttcgccgat
     2341 gtggaacaat ccgccttcgc accgagcaac ctcgttcccg gtgtcggcgc cagcccagat
     2401 aaaatgttgc aagcccgttt gttcaattac gccgacgcgc aacgctaccg cttgggcgtg
     2461 aacttccgcc aaatccctgt caaccgtccg cgttgccctg ttcacagcaa ccagcgcgac
     2521 ggacaaggac gcgccgacgg caactacggc agcctgccgc actacgaacc caacagcttc
     2581 ggccaatggc agcagcagcc cgacttcgcc gaaccgcctt tgaaaatcaa cggcgacgcg
     2641 gcacactggg actaccgcca agacgatgac gactatttca gccaaccgcg cgccctgttc
     2701 aacctgatga acgacgcgca gaaacaggca ttgttcggca acaccgccgc cgcaatgggc
     2761 gacgcgcccg acttcatcaa ataccgccat atccgcaact gctaccgttg cgatccggca
     2821 tacggcgaag gcgtggcaaa agccctcgga ctgactgtcg aagacgcaca agccgcccgc
     2881 gcgaccgacc ccgcactcgg ccagcccggt ttgctgtaag ggggcattat gtggatggaa
     2941 attgaagaaa tcctgtcccg cgccgtccgc taccggaaat aaccgggcat aaaaatgccg
     3001 tctgaaacgt cgtttgaccg cttcagacgg catttcccca tcccgccccc gccgtttcag
     3061 tgggcgtttt ttattaaacg caaaatatcc cgtcattccc gccccacagc ccacttttcc
     3121 gccgtcattc ccgcaccatc cccaccggtt caaaccggca cgaaaacttt tccgcgtcat
     3181 tcccgcgaaa gcgggaatct agaacgtaaa atctaaagaa accgttttat ccaataagtt
     3241 tc
//
LOCUS       DAXHRI010000179         3239 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXHRI010000179 DAXHRI010000000
VERSION     DAXHRI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.179"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(126..1226)
                     /locus_tag="ACFM75_001911"
     CDS             complement(126..1226)
                     /locus_tag="ACFM75_001911"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC6256399.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAEYFGTRVSLRSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSSHKATGASKPCILGAGYYY"
     gene            1551..3125
                     /locus_tag="ACFM75_001912"
     CDS             1551..3125
                     /locus_tag="ACFM75_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC6256400.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          674 a          807 c          939 g          819 t
ORIGIN      
        1 ttttcatggc agggtttgtt ttgttttgaa cgggtttgag tgtaaatgga ttttaagggt
       61 ttgtaaacga aaggggcgaa aatgccgtct gaacggcgga acgggctttc agacggtatt
      121 tgcgctcaat aataatatcc cgcgcccaga atacacggtt tggatgcgcc ggttgctttg
      181 tgcgaactac cgggaatgcg attaatccaa cacgccgcca accacgcaaa tgcggctgct
      241 tccacccatt gcggatcgag cttcagttcg gcggtgctgc gtaaggaaac gcgtgtgccg
      301 aaatattccg ccaaatccgc cattaaaaca ggattgcgga tgccgccgcc gcaaatgtac
      361 atttgacggg catctgccgc tgcgtgtgag acggcgtcgc aaacggtttg cgcggtaaaa
      421 cgggaaagcg tccgcaatac gtcgtatcgg ttttcaccgc cgtcaaggta ggtttcgagc
      481 caatttaggg caaacagttc gcgccccgtg cttttagggt ggggttgtgc gaaatacggg
      541 tgggcgagca gcctgtcgag cagttgcggc aatatgttgc cttgtgccgc ctttgcaccg
      601 tttttgtcgt aaggaagctg ccagtgtgcc tgcgtccacg cgtccatcag catattgccc
      661 ggccctgtgt cgaagccgaa ggcgggtgcg tcggagggga gtacgctgat gttggcaatc
      721 ccgccgatgt tcagtaccac gcgtgtttct ttgtcatcgc ggaacagggc tttgtgaaag
      781 gcggggacga gcggcgcacc ttgtccgccg gccgcaaggt cgcggctgcg gaagtcgccg
      841 acggtaaaaa tctgagtcag ttccgccagc agcggcaaat cggcaagctg tatgctgtaa
      901 ccgtgttccg gcgcgtgtcg gacggtttgc ccgtggcagc cgagggcggt aatgtcggac
      961 ggcgcgaggt tttgactgca cagcagttcg gcggcggttt gcgcgtacag gcggctgagt
     1021 tcttgcgaca aaatcctgct gcggtgcagt tcgtccgccc ccgtgtcctg caaatccagc
     1081 aattggcggc gcagcctgtc gggatagggg gtaaaggcgt gcccttccgc gcccagccat
     1141 ttgccgccgt ccatccgtac cagcacggca tccgccccat ccatactggt tcccgacatg
     1201 atgccgatgt aaagctgtgt ttccatcatc actcccaaac tggtgcaaaa cgccatttta
     1261 acgtgtattg acgctcgtat accgatttgc cgccgcagtg taaataaagt gtaaataaat
     1321 gtttcaagac ggatggaaaa atattataat gcgcccgcaa catccagtag tagaagtgtc
     1381 atacaaaccg tttccggcag cagttttgca ttcggtcagg tttgggggta ttcggatgcg
     1441 gttcagaagg aaggatgcgt ctgccatatc ccgaaacggc agttcgaccg gaggcagcag
     1501 tacagtgtcg gcaacactca tgatttccac cacattaaag gaagattgcc atggctcaaa
     1561 tccaaatgag cgcaaatgtt aaaaccatca acgccgtctt tgccgccatg ctggtaggta
     1621 cagtcggcta ttttatttat tggggcttgg gttataccca ttacaattac gccgccttat
     1681 tcattattgc cacgatgttc ggcgtgttta tggcgttcaa catcggcggc aacgatgttg
     1741 ccaattcttt cggcaccagt gtcggtgcgg gtacgctgac catcccgcag gctttgctga
     1801 ttgcggcggt atttgaggtc agcggcgcgg tcatcgcggg cggcgaggta accaatacca
     1861 tacgcaaagg catcgtcgat ttgaagggtg ttgatttcga acccatacag tttgtgttta
     1921 ttatgatgtc cgcgcttttg gcggcggcgt tgtggctgtt gtttgcctcg aaaaaagggc
     1981 ttccggtatc taccacccat tccattatcg gcggcattgt cggcagcgcg gtatgtatgg
     2041 cggtaatgaa cgatgccgca tcgggcgatt tgatacgttg gggcaagctg ggcggtattg
     2101 gtgtttcttg ggtattgtcg cccgtgttgg gcggcgcggt gtcctatttt ctgttttcgc
     2161 gcgtcaagaa aaacgtctta gattacaacg cttgggcgga aggcacgctc aagggcatca
     2221 agcaggaaaa aaaggcctat aaagaacggc accgcctgtt tttcgagggt ttgtccgaag
     2281 ccgaaaaagt cgagtacgcc accaaaatgg cgcacgacgc gcaaatttac gacgaacccg
     2341 aattcgatcc gcaagagctg caatcggagt attaccgcgg tctttatgcg ttcgacaacc
     2401 gtaaaaacaa tgtcgattcc tacaaagcgc tgcattcttg gattcccttt atcgcttcgt
     2461 tcggcgcgat gatgatttcc gctatgctga ttttcaaggg cttgaaaaac ctgcatttgg
     2521 ggatgagcaa cgtcaacagc ttcctgacca tctttatgat aggcgcggcg gtgtggatgg
     2581 ggacgtttgt gtttgccaaa agcctcaagc gtaaagactt gggcaaatcg acctttcaga
     2641 tgttttcatg gatgcaggtc tttaccgcct gcggcttcgc attcagccac ggtgcgaacg
     2701 atatcgccaa cgccatcggt ccgtttgccg cgattatgga tgttttgcgt accaacagcg
     2761 ttgccgcgca aaatgtcgtc cccccgattg cgatgctgac tttcggtatc gcgctgattg
     2821 tcggtttgtg gtttgtcggt aaagaggtga ttaaaaccgt cggtacgagt ttggcggaaa
     2881 tgcatcctgc ttcgggtttt accgccgaac tgtccgccgc ctccgtcgtg atgggcgcgt
     2941 cgctgatggg gctgcccgtg tccagtacgc atatcttggt cggcgcggta ctcggcatcg
     3001 gtctggtcaa ccgcaatgcc aactggaaac tgatgaagcc catcggtttg gcgtgggtca
     3061 ttaccctgcc tgccgccgcc gtattgtcgg ttgtctgcta cttggtttta caggcagtat
     3121 tctgattgta aaatactgat gccgtctgaa cccgtgttca gacggcattt tgttgatgga
     3181 atgtgcgggc ttgtgcctta tgcacaatct gttctgtcgg gatatgccgt ttggtatag
//
LOCUS       DAXHRI010000180         3224 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXHRI010000180 DAXHRI010000000
VERSION     DAXHRI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.180"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..142
                     /locus_tag="ACFM75_001913"
     CDS             <1..142
                     /locus_tag="ACFM75_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6256401.1"
                     /translation="VNPLYAGMAGLSGQISLTVPVLLTAQVLWVIIPLVLAAGIFRKR
                     QI"
     gene            139..633
                     /locus_tag="ACFM75_001914"
     CDS             139..633
                     /locus_tag="ACFM75_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC6256402.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVSFDDMPDT
                     YIFK"
     gene            complement(721..2382)
                     /locus_tag="ACFM75_001915"
     CDS             complement(721..2382)
                     /locus_tag="ACFM75_001915"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC6256403.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKNGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGMKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGFQSLPKLSFKGGVLVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(2512..3061)
                     /locus_tag="ACFM75_001916"
                     /pseudo
     CDS             complement(2512..3061)
                     /locus_tag="ACFM75_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(3006..3179)
                     /locus_tag="ACFM75_001917"
                     /pseudo
     CDS             complement(3006..3179)
                     /locus_tag="ACFM75_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          729 a          871 c          799 g          825 t
ORIGIN      
        1 tgttaatcca ctatatgcgg gtatggcggg tttgagcgga caaatcagcc tgaccgtccc
       61 cgttttgctg accgctcagg ttttatgggt tatcattccg cttgttttgg cagccggaat
      121 ttttagaaag cgacaaatat gaaaaaaacc ctgttggcaa ttgttgccgt ttccgcctta
      181 agtgcctgcc ggcaggcgga agaggggccg ccgcctttac cccggcagat tagcgaccgt
      241 tcggtcggac actattgcag tatgaacctg accgaacaca acggccccaa agcccagatt
      301 ttcttgaacg gcaaacccga tcagcccgtt tggttctcga ccatcaagca gatgttcggc
      361 tataccaagc tgcccgaaga gcctaaaggc atccgcgtga tttacgttac cgatatgggc
      421 aatgttaccg attggacgaa tcccaatgcc gacacggagt ggatggatgc gaaaaaagcc
      481 ttttacgtca tcgacagcgg ctttatcggc ggtatgggtg cggaagacgc gctaccgttc
      541 ggcaacaagg agcaggctga aaaatttgca aaggataaag gcggtaaggt tgtcagtttc
      601 gacgatatgc ctgataccta tattttcaaa taatattgaa aaaaccggcc gaaactttaa
      661 aaacgagttt cggccggttt ttcttttctt gttcggtata gtgtcggcag gaaagaacct
      721 tcacatcccg ccgtaattcg gcccgctcgc gccttcgggg caaatccaag tgatgttttg
      781 cgtcgggtct ttgatgtcgc aggttttgca gtgcacgcag tttgccgcgt tgatttgcag
      841 gcgcggattg ccgttttctt caacaatttc gtacacgccg gccggacaat agcgcgtttc
      901 gggcgaggcg tattctttgt agttcacgtc tatcatcgtt tgcggattgt tcagcaccaa
      961 atggtcgggc tggttttctt cgtgcgcgag attggcaagg aagacgctgc tcaagcggtc
     1021 gaaggtcaac acgccgtcgg gtttcggata atcaatcggc ttgcacgcgg cggctttttt
     1081 aagctgctcg ttgtctttgc cgtgatgttt caaggtccac ggggctttgc ctctgaaaat
     1141 catctgatcg atgccggtgt agattgagcc gaggtaaacg ccccatttga atgacggacg
     1201 gacattgcgc gcggcgtaaa gctcttgata cagccagctt tgttcaaaac gttgctgata
     1261 atccgccgcc tctttgccgc tgtcgaaacc ctccacttct tcaaggtttt ccaacaaggg
     1321 gaacacggct tcggcggcga gcatggcgga tttcatcgcg gtatgaatgc ctttgatgcg
     1381 cggcatattg aggaaacccg ccgcatcgcc gaccaaaacg ccgcctttga acgagagctt
     1441 cggcaaactt tgaaaaccgc cttcaatcag cgaacgcgca ccgtaagcaa tgcggcggcc
     1501 gccttcaaag gttttgcgga tttcgggatg ggttttgaaa cgttggaact cttcaaacgg
     1561 cgacagatac gggttttgat agtccaaacc gaccacgaaa ccgacggcga ctttgttgtc
     1621 gtcgaaatgg taaacaaacg cgccgccgta ggttttgctg tccagcggcc agcctgcgct
     1681 gtgcaccacc aaaccgggct gatgctgttc ggacggcact tcccaaactt ctttcatgcc
     1741 caagccgtaa gtttgcggct ggctgttttg gtcgagtttg aagcgttcga tgatttgttt
     1801 ggaaagcgaa cctcggcagc cctcggcaaa cagggtttgc cgtgcccaaa gctccatgcc
     1861 gggctggaat gaatcggtcg gctcgccgtt tttgcccacg cccatattgc cggttgcaat
     1921 gcctttgacc gaaccgtctt tgtgatacag cacttcggca gcggcaaagc ccggatagat
     1981 ttccacgccc atattttccg cctgctccgc caaccagcgc acgacttcgc ccaagctgac
     2041 gatgtagttg ccgtgattgt cgaaattggg ggtaatgggc agattgaacg cttttttctc
     2101 cgtcaggaac aacaccttgt cctgcgttac cgtgcgcgtc agcggtgcgc ctttttcttt
     2161 ccagtcggga atcagctcat tcagcgcaat cggatcgata accgcgcccg ccagcgaatg
     2221 cgcacctgct tcagaacctt tctccaccac gcaaacgctg atttcgcgcc cgttttgttc
     2281 ggcaagctgc ttgagtttga tggcggcaga caaacccgac gggcctgcgc cgacaatcac
     2341 gacatcgtat tgcatactgt cgcgggtgat ggattctgtc atggcggttc ctgtgtattt
     2401 attattgaat tgcaaatccg taattataca acgagaacat atagttacca aatacaacaa
     2461 aggtcgtctg aaaaccatat tttcggtttt cagacgacct ttgtcgaaat ttcaataagc
     2521 acgccaccat tttacctgtc cgaccgcaaa ctccgtctga cgtttcggac tgcgtgtgaa
     2581 aaacgcctta tccccgccgg catccctccc tttcggcacg accgccaaaa tcttacctgc
     2641 caaatttccc tcacgggttt gccaagcatc caaaaactgc gccctgctca ttgaaacatg
     2701 acccagcgac gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgagaa
     2761 atgatcatcc ttgcggtatt tcagatacac gatgacgggg atttgcaact gtgcaagctg
     2821 ctcgaaagac agggcatagc ctttcgcttc aaaacccaaa tcaggcataa tgcgccgcat
     2881 atcctcaaac gacgctggca tctgctcctt atccagtttt tttaacacgt cctcttccgt
     2941 cagcttttgc ccgtaaaaat tgttcaaaag ccgtcgccac cgaagccgcc ccgcaggaaa
     3001 aatccaaatc ctgctttaca atattgaaat cgcgcctttc tttccaactc tgcactttga
     3061 tttttccata agcaacagga ttatagtgga ttaaatttaa accagtacgg cgttgcctcg
     3121 ccttgccgta ctatctgtac tgtctgcggt ttcgtcgcct tgtcctgatt tttgttaatc
     3181 cactataggt ttccgtgcgg acgtgttcag attcccgcct tcgc
//
LOCUS       DAXHRI010000181         3140 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXHRI010000181 DAXHRI010000000
VERSION     DAXHRI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.181"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            231..2921
                     /gene="glnE"
                     /locus_tag="ACFM75_001918"
     CDS             231..2921
                     /gene="glnE"
                     /locus_tag="ACFM75_001918"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC6256404.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVWTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFTYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYSAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAELGIML
                     SEHVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYAAFSDDL
                     NAHRTKVNQLFNEILSEPEEQTLDTSEWQWAWQEKPDEEERQGRLKEHGFDAETIAAR
                     LDQIRNGHKYRHLSAHAQPRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNISADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
BASE COUNT          751 a          955 c          817 g          617 t
ORIGIN      
        1 tatgctattt taaaagagcg tggcgaggtg tgaacaggaa acggcggcat ttgccgctgt
       61 tttttatcgg caggcatccg tctggatgtc ggggcaaggt ttcagacggc atcgaaggtt
      121 gctatgatat agtggcttga ctttaaaccg gtacggcatc ccctcgccct gtcctgattt
      181 gaagtgaatc cgccatcatc ttcctgccat tttttcaaac ggaatccgaa atgtccgaca
      241 accgcctcga caccgcccgc cgccattccc tcttcctcgc ccgccagctc gacaacggca
      301 aactcaagcc cgaaattttc ctgcctatgc tggacaaggt ttggaccgaa gcggatttcc
      361 aagcctttgc cgactgggac aaaatccgtg cggaagaaaa cgaggaagaa ttggcgcggc
      421 agttgcgcgg gttgcgccgt tatgtggtgt cgcagattat cgtgcgcgat atcaaccgca
      481 tcagcgattt gaacgaagta acccgcacga ttacgctgtt tgccgatttt gccgtcaata
      541 ccgcgctgga ttttgcctac gcctattatc gggacatgta cggcacgccg atcgggcgtt
      601 ataccaaatc gccgcagcat ttgagcgtgg tggcgatggg caaggcgggc ggctatgagt
      661 tgaacgtgtc ttccgacatc gatttgattt tcacctatcc cgaatcgggc gacaccgacg
      721 gcaggcgcga acggggcaat caagagtttt tcaccaaagt cgggcagaaa ctgattgcgc
      781 tgctgaacga catcaccgcc gacggacagg tgttccgcgt cgatatgcgg ctgcggccgg
      841 acggtgattc gggcgcgttg gtattgagcg aaaccgcgct ggagcaatat ttgattacgc
      901 aggggcgcga atgggaacgc tacgcgtggt gcaaaggccg cgtggttacg ccgtatccga
      961 acggaatcaa atcgctggtg cgccctttcg tgttccgcaa atatttggat tacagcgcgt
     1021 atgaggcgat gcgtaagctg caccgccaaa tcagcagcga agtcagcaaa aaaggcatgg
     1081 cggacaacat caaactcggc gcgggcggca tccgcgaagt cgaatttatc gcccagattt
     1141 tccagatgat acgcggcgga caaatgcgcg cgctgcaact gaaaggcacg caggaaacgc
     1201 tgaagaagct tgccgagctg ggcatcatgc tgtctgaaca cgtcgaaacc ctgcttgccg
     1261 cctaccgatt cctgcgcgat gtcgaacacc gcctgcaata ctgggatgac cagcaaaccc
     1321 aaaccctgcc cacttcgcct gaacagcggc aactgctcgc tgaaagcatg ggtttcgaca
     1381 gctacgccgc tttttcagac gacctcaatg cccaccgcac caaagtcaat cagttgttta
     1441 acgaaatttt gagcgaaccc gaagagcaaa ccctagacac cagcgaatgg caatgggcat
     1501 ggcaggaaaa gcccgacgaa gaagagcggc aaggtcgtct gaaagaacac gggttcgatg
     1561 ccgaaaccat cgccgcaagg ctcgaccaaa tccgaaacgg tcataaatac cgccatctct
     1621 ccgcacatgc ccagccgcgt ttcgatgcgg ttgtgccgct gttcgtacag gcggcggcag
     1681 agcaaagcaa cccgaccgat acattgatgc ggctgttgga ttttctcgaa aacatcagcc
     1741 gccgatccgc ctatctcgcc ttcctcaacg aacatccgca aaccttggcg caattggcgc
     1801 agattatggg ccaaagttct tgggtggcgg cgtatctgaa caaatatccg attttgttgg
     1861 acgaactcat cagcgcgcag cttttggata ccgcgtttga ttggcaggcg ctcgccgccg
     1921 ccctttcaga cgacctcaaa gcctgcggcg gcgatactga agcgcaaatg gacaccctgc
     1981 gccgcttcca gcacgcccaa gtcttccgtc tcgccgtcca agacctcgcc ggactgtgga
     2041 cggtagaatc cctctccgac caactctccg ccctcgccga caccatcctc gccgccgccc
     2101 tgctgtgcgc atgggcggac atgcccaaaa aacaccgcga cacaccgcaa ttcgccgtcg
     2161 tcggctacgg caaactcggc ggtaaagaac tcggctacgc ctccgacctc gacctcgtct
     2221 acctctacga cgacccccac cccgacgcag gcgacgtgta cagccgcctc gcccgccgcc
     2281 tgaccaactg gctttccgcc gccactggcg caggcagcct ctacgaaacc gacctgcgcc
     2341 tgcgccctaa tggcgacgcc ggtttcctcg cccacagcat cgccgccttt gaaaaatacc
     2401 agcgcgaaaa cgcctggacg tgggaacacc aatcccttac ccgcgcccgc ttcatctgcg
     2461 gcacgcccga aattcagacg gccttcgacc gcatccgcac cgaaatcctc accgccgaac
     2521 gcgaccaaac cgccttgtca ggcgaaatca tcgaaatgcg cgaaaaaatg ttccccaccc
     2581 acccgcctgc cgacagcaac gtcaaatacg cacgcggcgg cgtggtcgat gtcgaattta
     2641 tcgtccaata tctgatactt gcccatgccc gccagtatcc gcaactcttg gacaactacg
     2701 gcaacatcgc cctcttaaac atctccgccg actgcggttt gattgacaaa accctcgccg
     2761 gacaaagccg caccgcctat cgcttctacc gccggcagca gcacaacacc aaactgcgcg
     2821 acgcggcaaa aaccgaagta agcgacgaat tactgtccca ttacggcaat gtcaggaaat
     2881 tgtggcggga agtgttcggc gaagaagcgg caaccgcctg aacaaaaaat gccgtctgaa
     2941 gcctgacaat ctgggtttca gacggtattt tcgtaccgtg ccgttttaag gttgcggcag
     3001 agctaaagcg atttatcggg aatggctgaa acccaaaaac cggattcctc tttcgcggga
     3061 atgacgggat ttcaggcttc tgtttttgtg ggaatgatgg gattttttat ccaagcaaaa
     3121 atcaaaacaa acaaataaga
//
LOCUS       DAXHRI010000182         3130 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXHRI010000182 DAXHRI010000000
VERSION     DAXHRI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.182"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            1..1363
                     /locus_tag="ACFM75_001919"
                     /pseudo
     CDS             1..1363
                     /locus_tag="ACFM75_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            1487..3037
                     /locus_tag="ACFM75_001920"
     CDS             1487..3037
                     /locus_tag="ACFM75_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC6256405.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT          876 a          744 c          794 g          716 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcaccgatt accgtcgtca attacgacga acaagccctc
       61 aataacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gcgaaagcaa cacgctgacc ggactgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat accggtacgg cccgcctgac cgaccaatac ttccgcactt taagcatgaa
      241 cctgtctgca cgcggcgaat ttgaaaccgg cccggtaaca cataactgga gtatggcttt
      301 cgaccgcgtt atccgccagc ggaaaaccat tcgcggaacg gcagccggag ctagcaaggt
      361 agaagtgaag gcaaacggaa atattgagaa tcaattggca tcgtttacag ccgactatcc
      421 caattcttgg gagaactctg ccaacttgga tgccaatatc aaagtcaaca gcctggcttt
      481 gtccgacacc ttgggcttcg tggataacaa ataccgcctg actttgggcg gacgtttcca
      541 agcggttgaa tacaccgaca agaaaaaatc acagagcggt aatgccaaac gcttcagccc
      601 gatgctgatg gcggcttggg tgccgcagcc cgatttggtg gtttacggca actatatgga
      661 agacttggag cctgccgaca ttaaaaccga tgattccggt gaaacgacaa tggcgaaacc
      721 gcgcgtcagc cgccagtttg aagtgggcgt tcgtaaaaac tggggcgact ttgttacgac
      781 gttgaacgcg tttcaaatca aacgcccggg ctactggcgc ggcaatacca aaaaaggtac
      841 tgattttgca gcatataaag cagcaggagg cgcagacgga gatgaacaag gcatggaacg
      901 cagccgcggt atcgaattca atgcctatgc caacctgtta aacaaaaccc tgcgcccgac
      961 tttcggcctg atgtacctgc aatcgaccgt gaaagactac ccaaattcac gcgatatgct
     1021 ggttaacggc gtacaagtcg ccaatccgcg cgtaattgcc aaggcaggtt tggaatggga
     1081 tacaccgttt gccaaaggct tgactttaaa cagcaatgtt tcgtatttcg gcaagtctta
     1141 ccaagacacg caaaaacaat acgccttccc atcctatacc ttggttgatg taggcgcgcg
     1201 ctacaaaacc aagctcggca aaaatacgtt gaccgtcagc agctcggtgg aaaatctgtt
     1261 caacaaaaac tactggcagg tacagcgcgg ccaatacgac cgcagcttcg ccgtcgtcgg
     1321 tctgccgcgt acttactggc tgaaggcgga attggatttc tgatttctcc gcatctggaa
     1381 aatgccgtcc gaacaggttt cagacggcat ttcttttaaa gagatatggc gggatacgct
     1441 taaataatgc tattctagcg tctttgaaac ctgcttgttt gaagtgatga aaaaaccttt
     1501 gatttcggtt gcggcagcat tgctcggcgt tgctttgggc acgccttatt atttgggtgt
     1561 caaagccgaa gaaagcttga cgcagcagca aaaaatattg caggaagcgg gcttcttgac
     1621 cgtcgaatcg caccaatatg agcgcggctg gtttacctct acggaaacga cggtcatccg
     1681 cttgaaaccg gagttgctga ataatgcccg gaaatacctg ccggacaacc tgaaaacagt
     1741 gttggaacag ccggttacgc tggtaaacca tatcacgcac ggccctttcg ccggcggatt
     1801 cggcacgcag gcgtacattg aaaccgagtt caaatacgcg cctgaaacgg aaaaagttct
     1861 ggaacgcttt tttggaaaac aagtcccggt ttcccttgcc aataccgttt attttaacgg
     1921 cagcggtaaa atggaagtca gtgttcccgc cttcgattat gaagagctgt cgggcatcag
     1981 gctgcactgg gaaggattga cgggaaaaac ggtttatcaa aaaggtttca aaagctaccg
     2041 caacagctat gatgcgccct tgttcaaaat caagctggca gacaaaggcg atgccgcgtt
     2101 tgaaaaagcg catttcgatt cggaaacttc agacggtatc aatccgcttg ctttgggcag
     2161 cagcaatctg accttggaaa aattctccct agaatggaaa gagggtgtcg attacaacgt
     2221 caagttaaac gaactggtca atcttgttac cgatttgcag attggcgcgt ttatcaatcc
     2281 caacggcagc atcgcacctt ccaaaatcga agtcggcaaa ctggcttttt caaccaagac
     2341 cggggaatca ggcgcgttta tcgacagtga agggcagttc cgttttggca cactggttta
     2401 cggcgatgaa aaatacggcc cgctggatat tcatatcgct gccgaacacc tcgatgcttc
     2461 tgccttaacc gtattgaaac gcaagtttgc acaaatttct gccaaaaaaa tgactgagga
     2521 acaaatccgc aatgatttga ttgccgccgt caaaggcgag gcttctggac tgttcaccaa
     2581 caatcccgta ttggacatta aaacttttcg tttcacgctg ccgtcaggaa aaatcgatgt
     2641 gggcggaaaa atcatgttta aagatttgaa gaaggaagat ttgaaccaat tgggtttgat
     2701 gctgaagaaa accgaggcag acatcagaat gagtattcct caaaaaatgt tggaagactt
     2761 ggcggtcagt caggcaggca atattttcag cgtcaatgcc gaagatgagg cggaaggcag
     2821 ggcaagtctt gacgacatca acgagacctt gcgcctgatg gtggacagta cggttcagag
     2881 tatggcaagg gaaaaatatc tgactttgaa cggcgaccag attgatactg ccatttctct
     2941 gaaaaacaat cagttgaaat tgaacggtaa aacgttgcaa aacgaaccgg agccggattt
     3001 tgatgaaggc ggtatggttt cagagccgca gcagtaatgg gtcaaatgcc gtctgaaatg
     3061 ttcaggcggc attttgttgt attggaaact gccgttttca accattcctt tagataagcc
     3121 gtgtcggatt
//
LOCUS       DAXHRI010000183         3121 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXHRI010000183 DAXHRI010000000
VERSION     DAXHRI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.183"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..561
                     /locus_tag="ACFM75_001921"
     CDS             <1..561
                     /locus_tag="ACFM75_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFC6256406.1"
                     /translation="KSMVERILTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGI
                     PNNLLPYICQVAAGKLPQLAVFGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVA
                     GTHLLNLGSGRASSVLEIIRAFEAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGW
                     QTQRDLTQMMEDSWRWVSNNPNGYDD"
     gene            677..1744
                     /gene="rfbB"
                     /locus_tag="ACFM75_001922"
     CDS             677..1744
                     /gene="rfbB"
                     /locus_tag="ACFM75_001922"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC6256407.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAGNLESL
                     TDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNI
                     VGTFNLLEAARAYWQQMPSEQREAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVRTICALLEELVPN
                     KPQGVARYEDLITYVKDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDN
                     KTWWQNVLNGSYRLERLGTGK"
     gene            1807..2673
                     /gene="rfbA"
                     /locus_tag="ACFM75_001923"
     CDS             1807..2673
                     /gene="rfbA"
                     /locus_tag="ACFM75_001923"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6256408.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKNVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            2718..>3121
                     /gene="rfbC"
                     /locus_tag="ACFM75_001924"
     CDS             2718..>3121
                     /gene="rfbC"
                     /locus_tag="ACFM75_001924"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6256409.1"
                     /translation="MNIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFYVLSDTTELTYKCTD"
BASE COUNT          788 a          853 c          775 g          705 t
ORIGIN      
        1 aaatcgatgg ttgagcgcat tctcactgac attcaaaaag ccgatccgcg ctggagcatg
       61 attttgttgc gttatttcaa tccaattggc gcgcatgaaa gcggcttgat tggcgagcag
      121 ccaaacggta tcccaaataa tttgttgcct tatatctgcc aagtggcggc aggcaaactg
      181 ccgcaattgg cggtatttgg cgatgactac cctacccccg acggcacggg gatgcgtgac
      241 tatattcatg tgatggattt ggcagaaggc catgtcgcgg ctatgcaggc gaaaagcaat
      301 gtagcaggca cgcatttgtt gaacttaggt tccggccgcg cttcttcggt attggaaatc
      361 atccgcgcat ttgaagcggc ttcgggttta accattccat ttgaaatcaa accgcgccgc
      421 gccggtgatt tggcgtgctt ctatgccgac ccttcttata cgaaggcgca aatcggctgg
      481 caaacgcagc gtgatttaac ccaaatgatg gaagattcat ggcgctgggt aagtaataat
      541 ccgaatggct acgacgatta agttgacctg atacaggccg tctgaaagag atgttttcag
      601 acggcctctt tatctgaaaa acacacattc tgtctgctat aatctgttta tattttttgg
      661 ctatcctctg aaatttatga gaaaaatcct cgttaccggc ggtgcgggct ttatcggttc
      721 tgccgttgtc cgtcatatca ttcaaaacac ccaagattcc gtcgtcaacc tcgacaaact
      781 gacctacgcg ggcaatctcg aatcgctgac cgacattgcc gataatcccc gctatgcttt
      841 cgagcaagtg gacatttgcg accgcgccga actcgaccgc gtattcgcac aacaccggcc
      901 cgatgccgtg atgcacttgg cggcggaaag ccatgtcgac cgctctatcg gttcggcagg
      961 cgagtttatc caaaccaata tcgtcggcac attcaatctg cttgaagcag cccgcgccta
     1021 ctggcaacaa atgccgtctg aacagcgcga agctttccgt ttccaccata tttccaccga
     1081 tgaagtctat ggcgatttag gcggcacgga cgatttgttt accgaaaccg cgccctacgc
     1141 gccgtccagc ccctactctg cctctaaagc gtccagcgac cacctcgtcc gcgcgtggtt
     1201 gcgtacttac ggcttgccga ccattgtaac caactgctcc aacaactacg gtccttacca
     1261 ttttccggaa aaactcattc ctttgatgat tctgaacgcg cttgacggca aaccgctgcc
     1321 tgtgtacggc gacggtatgc aaatccgcga ctggctgttt gtcgaagacc acgcgcgcgc
     1381 actgtatcag gttgttaccg aaggtgttgt cggcgaaacc tacaatatcg gcggccacaa
     1441 tgaaaaagcc aatattgaag tggtgcgcac catttgcgcc ctgctggaag aattggtgcc
     1501 gaacaaaccg caaggcgtgg cgcgttatga agatttgatt acctatgtca aagaccgccc
     1561 cggccatgat gtgcgctacg ccgtcgacgc agccaaaatc aggcgggatt tgggctggct
     1621 gcctttggaa accttcgagt ccggcctccg caaaaccgtg caatggtatc tggacaacaa
     1681 aacctggtgg caaaatgtat tgaacggcag ctatcgtttg gaacgtttag gtactggaaa
     1741 atagttttca gacggcatcc cgacgcaatg ccgtctgaaa acccatcgca aaggaagaaa
     1801 gaaaagatga aaggcatcat actggcaggc ggcagcggca cgcgcctcta ccccatcacg
     1861 cgcggcgtat ccaaacagct cctgcccgtg tacgacaaac cgatgattta ttaccccttg
     1921 tcggttttga tgctggcggg aatccgcgat attttggtga ttaccgcgcc tgaagacaac
     1981 gcctctttca aacgcctgct tggcgacggc agcgatttcg gcatttccat cagttatgcc
     2041 gtgcaaccca gtccggacgg cttggcacag gcatttatca tcggcgaaga atttatcggc
     2101 aacgacaatg tttgcttggt tttgggcgac aatatttttt acggtcagtc gtttacgcaa
     2161 acattgaaac aggcggcagc gcaaacgcac ggcgcaaccg tgtttgctta tcaggtcaaa
     2221 aaccccgaac gtttcggcgt ggttgaattt aacgaaaact tccgcgccgt ttccatcgaa
     2281 gaaaaaccgc aacggcccaa atccgattgg gcggtaaccg gcttgtactt ctacgacaac
     2341 cgtgccgtcg agttcgccaa acaaatcaaa ccgtccgcac gcggcgaatt ggaaattacc
     2401 gacctcaacc ggatgtattt ggaagacggc tcgctctccg ttcaaatatt gggacgcggt
     2461 ttcgcgtggc tggacaccgg tacgcacgaa agcctgcacg aggcggcttc gttcgtccaa
     2521 accgtgcaaa acatccaaaa cctgcacatc gcctgcctgg aagaaatcgc ttggcgcaac
     2581 ggttggctga cgaaaaaaaa tgtggaaaca cgggcaaagc ctttggaaaa aaccgcctac
     2641 gggcaatacc tgctgcgcct aatcggcaaa taaatgccgt ctgaaccact ttcttcagac
     2701 ggcatacaga gaaaatcatg aacatcatcg acaccgccct ccccgacgta aaacttttaa
     2761 aaccgcaagt cttcacagac gggcgcggct tttttatgga aaccttccgc gacgggtggt
     2821 ttaaaaccca agtctgcgaa cgcaccttcg tgcaggaaaa ccactccaaa tccggcaaag
     2881 gcgtattgcg cggcctgcac taccaaaccg aaaacacaca aggcaaactc gtacgcgtag
     2941 ttgtcggcga agtgttcgac gtggccgtcg atatgcgtaa agactccccc actttcggca
     3001 aatgggtagg cgagattctg tccgcagaaa acaaacgcca actgtgggta cccgaaggtt
     3061 tcgcgcacgg gttttacgtt ttgagcgaca cgaccgaact tacctacaaa tgcacggact
     3121 a
//
LOCUS       DAXHRI010000184         3117 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXHRI010000184 DAXHRI010000000
VERSION     DAXHRI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.184"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..423
                     /gene="mafB"
                     /locus_tag="ACFM75_001925"
     CDS             <1..423
                     /gene="mafB"
                     /locus_tag="ACFM75_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6256410.1"
                     /translation="PPKPSTQQHPTHSDNNIGLPASYVKPDTSISPTGTIQDRIRWTK
                     SKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHRRVRYDI
                     NNNIYVLANPKTFKIKTMFKPNLGKKYYDGEFKKDMGN"
     gene            389..595
                     /locus_tag="ACFM75_001926"
     CDS             389..595
                     /locus_tag="ACFM75_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC6256411.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            616..885
                     /locus_tag="ACFM75_001927"
     CDS             616..885
                     /locus_tag="ACFM75_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256412.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTY"
     gene            908..1210
                     /gene="imm40"
                     /locus_tag="ACFM75_001928"
     CDS             908..1210
                     /gene="imm40"
                     /locus_tag="ACFM75_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HFC6256413.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            1294..1647
                     /locus_tag="ACFM75_001929"
                     /pseudo
     CDS             1294..1647
                     /locus_tag="ACFM75_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1645..1905
                     /locus_tag="ACFM75_001930"
     CDS             1645..1905
                     /locus_tag="ACFM75_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6256414.1"
                     /translation="MRPTTRDELRQALQEQGFRRTGSDEAQYETWKGPDGVKIDIRPN
                     GEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNRHHSGYFVK"
     gene            1902..2219
                     /locus_tag="ACFM75_001931"
     CDS             1902..2219
                     /locus_tag="ACFM75_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256415.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            2289..2624
                     /locus_tag="ACFM75_001932"
                     /pseudo
     CDS             2289..2624
                     /locus_tag="ACFM75_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            2598..3041
                     /locus_tag="ACFM75_001933"
     CDS             2598..3041
                     /locus_tag="ACFM75_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256416.1"
                     /translation="MASGNRPPKSITSEGKANAATYPQLVNQLTEQNLKNIAAQDSRL
                     ASAVNDWKTIQPNKKGEINFGIGSATRQEAEQLGKIWVGDGAKPVNSPSCQGCMLSAD
                     GTRLYRPPTTKSNTPESLNPTGVQANFVTRSVDGKTLTNGHLNIK"
BASE COUNT         1132 a          535 c          606 g          844 t
ORIGIN      
        1 ccaccaaaac caagtacgca gcaacaccct acacactctg ataacaatat cggcttacct
       61 gcctcatatg ttaaacctga tacatctatt tctccgacag gaacaattca agaccgcatc
      121 agatggacaa agtccaagtt tcctactgag aaatctttaa atggacattt caaagctcat
      181 ggaaaagaat ttggcgatat aaccattgaa gactaccaaa aaatggcgtc tgatttgtta
      241 tcaaaacaga catcggacaa gatattaggt tatcagacgg aacatagacg agtgcgctat
      301 gatatcaata acaatatcta tgttttggcc aatccaaaaa cattcaaaat caaaacaatg
      361 tttaaaccaa acttaggaaa gaagtattat gatggagaat tcaaaaaaga catgggaaat
      421 tgacggagaa atatggctac attgtcctgt ttgcggaact gaagttatgg actatgatat
      481 ctgtgacgtt tgtcagtggc aaaatacagg agaaactaat atagatggtg gccctaatga
      541 aatgacactt gcggaggcga aagaagctta cgcaaaaggc ttaccaatca gataaataag
      601 cacctagaga aatcaatgat gacggaatcc catggttacc tatcaaataa tttacctgtt
      661 aaaatcataa atgatattat ttatgcaacc cagttagtcg aagatttggt tttaggaaaa
      721 ataaaaattg ttgatttttt aaaatcatat aacaattttt attgttggct tggttttgat
      781 gagttgcctc aatctgagaa aataaaattc ctaagctatc tcaatatatt aagtattcat
      841 aaagaaatac aagatgaaac tgtgaatagg gtttatacct attgaaaaat agtagataga
      901 gattaacatg ttaaatgaaa tttttgaaat ttattcgaga caaggggaat ctttgatagg
      961 aattggaatt agagaagccg cattacccgt ccctattgca atagatatat taaatttatt
     1021 tatcaatgag agaatacttg tattgggggg agatatttat atcaagaaag ataattattt
     1081 ttatcaaaca tatgataatt ggtattacga gggaagtaat ttatttaaca gtatcgacaa
     1141 agcaatgcat tatttatctc aaataaaatt agagaatgca tacgtatctt ttgtgttgaa
     1201 atttatctaa caaaggaagc acaagaatag atttatagta aaacatcaag atgttgaaaa
     1261 tgctgggttt taatccaacc tacactgacc ggctcagata cagccgctca gcagactcta
     1321 caaggtatta atgatttagg aaaattaagt ccggaagcac aacttgctgc cgcgagccta
     1381 ttacaggaca gtgcttttgc ggtaaaagac ggcatcaatt ccgccagaca atgggctgat
     1441 gcccatccga atataactgc aacagcccaa actgctcttg ccatagcaga ggccgcaggt
     1501 acggtttggg gcggtaaaaa agtaaacctt aacccgacca aatgggattg ggttaaaaat
     1561 accggctata aaaaacctgc tgcccgacct atgcagacgt tggacggtga gatggcagga
     1621 ggaaacaagc cagttgttaa atctatcaga ccaactacgc gagatgaatt acgtcaagca
     1681 ttgcaagaac aaggttttag acgtactggt tcagatgagg ctcaatatga aacatggaaa
     1741 ggtcctgatg gcgtgaaaat agatattcgt ccaaatggag aggttataag aacccaaaga
     1801 gtgccgcgaa ccgatggtgt acagggaaaa tatccgcaac gacaagatta tgaaggcaat
     1861 ccattgccaa ataatcgtca tcattctgga tattttgtca aatgaaaaaa aatatttttc
     1921 acaatgtaag cctttatgaa ataatctttt ccgataatgg aaataccctt acattatctt
     1981 ttacagatac aattgaaggt aattatttcg gatatatcaa atgcagtaat attttgaatt
     2041 ttaaattaga tacaaataat ttcgtagatt atgaggataa ggaagatagc ttgtttccct
     2101 tgtttatacc cgaaatagag ctatataaat accaatttta tagtgaaatt attattgatg
     2161 tagggattat tataaaaata tctgctgaaa caattaattt tgagccactg ggaaaatagt
     2221 aactgctttc ccagcagccg tagcaactgt atttttaccc gacggggtaa aaatacagtt
     2281 gctacatctc tgcgcagcag actctacaag gtattaataa tttaggaaat ttaagtcccg
     2341 aagcacaact tgcggccgca accgcattac aagacagtgc ttttgcggta aaagacagta
     2401 tcaactctgc cagacaatgg gctgatgccc atccgaatat aactgcaaca gcccaaactg
     2461 ccctttccgt agcagaggcc gcaggtacgg tttggagagg taaaaaagta gaacttaacc
     2521 cgactaaatg ggattgggtt aaaaataccg gctataaaaa acctgctgcc cgccctatgc
     2581 agactttaga tggggagatg gcaagtggga atagaccgcc taaatctata acgtcggaag
     2641 gaaaagccaa tgctgcaact tatcctcaat tagttaatca attaactgag caaaacttaa
     2701 aaaacattgc tgcacaagat tcaaggttag caagtgctgt aaatgattgg aaaacaatac
     2761 aaccaaataa aaagggagaa attaattttg gtattggttc tgcaactcgt caagaggctg
     2821 aacagttagg aaaaatttgg gttggagacg gagctaaacc agttaatagt ccatcttgtc
     2881 aaggatgtat gttaagtgca gatggcacaa ggctatatcg tcctccaact acaaaatcaa
     2941 acacccctga atcacttaac ccaacgggag tgcaagctaa ttttgttact agaagtgttg
     3001 atggaaaaac cttaactaat ggacatttga atataaaatg attaaattgg atttaaaaag
     3061 cataaattta tatgatattg actttgaaaa atttaccccc gaaattccag ataattt
//
LOCUS       DAXHRI010000185         3096 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXHRI010000185 DAXHRI010000000
VERSION     DAXHRI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.185"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..335
                     /locus_tag="ACFM75_001934"
     CDS             <1..335
                     /locus_tag="ACFM75_001934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256417.1"
                     /translation="KTAENLCWGFGCREGKEFCKGLKQKQKPKVPSFPRRRESRPPTR
                     QESIGNNRNRTNLDSRFRGNDGGKVAVFPINSCRSSFLGWREIKQKRAYQKTTMQRTA
                     LAILRFAP"
     gene            complement(411..2045)
                     /gene="groL"
                     /locus_tag="ACFM75_001935"
     CDS             complement(411..2045)
                     /gene="groL"
                     /locus_tag="ACFM75_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC6256418.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGQVISEEVGLSLEKATLEDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(2138..2428)
                     /locus_tag="ACFM75_001936"
     CDS             complement(2138..2428)
                     /locus_tag="ACFM75_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC6256419.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            2657..>3096
                     /locus_tag="ACFM75_001937"
     CDS             2657..>3096
                     /locus_tag="ACFM75_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6256420.1"
                     /translation="MKITHCKLKKKVQKELLRFFALEITARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNRRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLDVSGFI"
BASE COUNT          674 a          802 c          808 g          812 t
ORIGIN      
        1 cgaaaacagc cgaaaacctg tgttggggtt tcggctgtcg ggagggaaag gaattttgca
       61 aaggtctcaa acaaaaacag aaacctaaag tcccgtcatt cccgcgcagg cgggaatcca
      121 gaccaccaac gcggcaggaa tctatcggaa ataaccgaaa ccggacgaac ctagattccc
      181 gctttcgcgg gaatgacggc ggaaaggttg ctgtttttcc gataaattcc tgccgctctt
      241 cgtttttggg atggcgggaa ataaaacaaa agcgcgcgta tcaaaaaaca acaatgcaaa
      301 gaacggcgtt ggcaatttta aggtttgcgc cttagccgca cttattcgca aaaaaaaccg
      361 cacggcgttg accgtgcggc ttgttgtctg aaggcttcag acggcattgc ttacatcatg
      421 ccacccatac cgcccatgcc gcccatatcg ggcatagcag gtttttcttc agggatttcg
      481 gcaatcatgc aatcggtcgt cagcatcagg ccggcgatgg acgcggcgtg ttgcagcgcg
      541 gaacgggtta ctttggcggg gtcgagtacg cccatttcga tcatgtcgcc gtattcgccg
      601 gagcctgcgt tgtaaccgta gttgcctttg ccttccaaca ctttgttcac caccacgcta
      661 ggttcgccgc ctgcgttggc aacgatttgg cgcagcggag actcaacggc gcgcaagacg
      721 atttgtacgc ctgcgtcttg gtcggcattg ccggtgtgca ggttttccag agcggcacgg
      781 gcgcgcaaca gggctacgcc gccgcctgca accacgcctt cttcaacggc tgcgcgggta
      841 gcgtgcagcg cgtcttccac gcggtctttt ttctctttca tttcgacttc ggtagcggca
      901 ccgaccttga ttactgccac gccgcctgcc agtttggcaa cgcgctcttg cagtttttct
      961 ttgtcgtaat cgctggttgc ggtttcgatt tgttggcgga tttcggcaac acgcgcttcg
     1021 atttgggcag cgtcgccgaa gccgtcgatg atggtggtgt tttctttacc gatttcgatg
     1081 cgtttggctt gacccaaatc ttccaaagtg gctttttcca aagacaagcc cacttcttcc
     1141 gaaatcactt ggccaccggt caaaatggcg atgtcttgca acatggcttt gcggcggtcg
     1201 ccgaagcccg gagctttaac ggcaacggtt ttcagaatgc cgcggatgtt gttcacgacc
     1261 aaagtcgcca aggcttcgcc ttctacgtct tcagcgataa tcaacagcgg acggctggct
     1321 ttggccactt gttccaaaac aggcagcagg tcgcggatat tgctgatttt tttgtcgaac
     1381 agcaatacaa acggattgtc caagccggcg atttgttttt ccgcatcgtt gatgaagtaa
     1441 ggagacaggt agccgcggtc gaattgcata ccttcaacca catccagctc gttttccaaa
     1501 gatttgccgt cttcaacggt aatcacgcct tctttgccga ctttttccat cgcttcggcg
     1561 ataatcgcgc cgacttgttc gtcagagttg gcggaaatcg agccgacttg ggcgatttct
     1621 ttagaagtgt cgcaaggttt ggcgatgttt ttcagctcgt caaccaaagc ggcaacggct
     1681 ttgtcgatac cgcgtttcag gtcggtcggg ttcataccgg cggtaacgta tttcatacct
     1741 tcggcaacga tggattgcgc caatacggtg gcggtagtcg taccgtcgcc cgccacgtcg
     1801 ttggttttgg acgcgacttc tttcaccatt tgcgcgccca tattttcaaa cttgtctttc
     1861 aattcgattt ctttggcgac ggttacgccg tctttggtga tgtgcgggcc gccgaatgcg
     1921 cggtcaacca ccacgttgcg gcctttggga cccaaggtta cgcgtacggc gtttgccaga
     1981 atgttcacgc cgtttaccat tttttgacgg acttcattgc cgaactgtac gtcttttgct
     2041 gccatttcaa ttctccaaaa atcattaaaa ctgtctgata aaaccgttta tgccgtctga
     2101 aggcggtttg ccgtttcaga cggcatcgtg tccgtattta tttttcaacg atgccgaaaa
     2161 tatcttcttc gcgcattacc aacagctctt cgccgtcggc ttttacggtt tggccgctgt
     2221 atttgccgaa gatgattttg tcgccgactt tgacatccag cggacggcgc gcgccgtctt
     2281 taccgatttt gcccgcgccc acggcgatga cttcgcccat gtcgggtttt tcggcggccg
     2341 cacccggcaa aacgatgccc gatgcggttt tctcttcagc ttccaagcgt ttgacgacaa
     2401 cgcggtcgtg taaaggacgg atggtcatat ttatgctccg ataaaatgat ttgaaaacaa
     2461 tcatctgccc gaacggttcg gacagattga agtggaaacc gggctgccgc ctgccggccc
     2521 gcccgtataa gtcggcaaaa ttagggtgtg tgcgggcaaa ttcaagtgag gcggaaaaaa
     2581 tttatttccg ccgtttttta tagtggacta aatttaaggg gctgtactag attagcagat
     2641 atgttaccct cgaaatatga agataacaca ctgcaaatta aagaaaaaag tacagaaaga
     2701 actgctccgt ttttttgcgc tggaaattac cgcccgttct gccgccgata ttttgggtat
     2761 ccatcccaat tcggcagcac tgttctaccg taaaatccgc acggttatca accatcattt
     2821 ggccttggct gccgatgagg tttttgaggg ccctgtcgag ccggacgaaa gcgatttcgg
     2881 cggacggcgt aaaggtagac gtggtcgcgg tgcagcagga aaagtggttg ttttcggcat
     2941 tctgaaacgc aacagacggg tctataccgt tgtggtggat aatgccaagt ctgaaacgtt
     3001 actccctgtc atcaaaaaga aaatcatgcc ggacagtatt gtttataccg atagtctgag
     3061 cagctatgac aagctggacg taagcggttt tatcca
//
LOCUS       DAXHRI010000186         3082 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXHRI010000186 DAXHRI010000000
VERSION     DAXHRI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.186"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            112..2880
                     /gene="tdfH"
                     /locus_tag="ACFM75_001938"
     CDS             112..2880
                     /gene="tdfH"
                     /locus_tag="ACFM75_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC6256421.1"
                     /translation="MKSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGGLKFNSNSGKWER
                     DFQRPYWKTKWYQKYNDPQELQKYIEGHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYREHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEEVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          865 a          769 c          752 g          696 t
ORIGIN      
        1 caattgaaat cttcagacgg tatatcgaat ttacactttt taatgtttat gccgcctaaa
       61 aaaatgctaa tatatttctt aattgtctga ctgtttattg ttgaggaaaa tatgaaatct
      121 tctttccggt tgaagccgat ttgtttttat cttatgggtg ttatgctata tcatcatagt
      181 tatgccgaag atgcagggcg cgcgggcagc gaggcgcaga tacaggtttt ggaagatgtg
      241 cacgtcaagg cgaagcgcgt accgaaagac aaaaaagtgt ttaccgatgc gcgtgccgta
      301 tcgacccgtc aggatatatt caaatccagc gaaaacctcg acaacatcgt acgcagcatc
      361 cccggtgcgt ttacacagca agataaaagc tcgggcattg tgtctttgaa tattcgcggc
      421 gacagcgggt tcgggcgggt caatacgatg gtggacggca tcacgcagac cttttattcg
      481 acttctaccg atgcgggcag ggcaggcggt tcatctcaat tcggtgcatc tgtcgacagc
      541 aattttattg ccggactgga tgtcgtcaaa ggcagcttca gcggctcggc aggcatcaac
      601 agccttgccg gttcggcgaa tctgcggact ttaggcgtgg atgacgtcgt tcagggcaat
      661 aatacctacg gcctgctgct aaaaggtctg accggcacca attcaaccaa aggtaatgcg
      721 atggcggcga taggtgcgcg caaatggctg gaaagcggag catctgtcgg tgtgctttac
      781 gggcacagca ggcgcagcgt ggcgcaaaat taccgcgtgg gcggcggcgg gcagcacatc
      841 ggaaattttg gcgcggaata tctggaacgg cgcaagcagc gatattttgt acaagaaggc
      901 gggttgaaat tcaattccaa cagcggaaaa tgggagcggg atttccaaag gccgtactgg
      961 aaaaccaagt ggtatcaaaa atacaatgac ccccaagaac tgcaaaaata catcgaaggt
     1021 catgacaaaa gctggcggga aaacctggcg ccgcaatacg acatcacccc catcgatccg
     1081 tccagcctga agcagcagtc ggcaggtaat ctgtttaaat tggaatacga cggcgtattc
     1141 aataaataca cggcgcaatt tcgcgattta aacaccaaaa tcggcagccg caaaatcatc
     1201 aaccgcaatt atcaattcaa ttacggtttg tctttgaacc cgtataccaa cctcaatctg
     1261 accgcagcct acaattcggg caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg
     1321 gggcttttaa aggattttga aacctacaac aacgcaaaaa tcctcgacct caacaacacc
     1381 gccaccttcc ggctgccccg cgaaaccgag ttgcaaacca ctttgggctt caattatttc
     1441 cacaacgaat acggcaaaaa ccgctttcct gaagaattgg ggctgttttt cgacggtcct
     1501 gatcaggaca acgggcttta ttcctatttg gggcggttta agggcgataa agggctgctg
     1561 ccccaaaaat caaccattgt ccaaccggcc ggcagccaat atttcaacac gttctacttc
     1621 gatgccgcgc tcaaaaaaga catttaccgc ttaaactaca gcaccaatac cgtcggctac
     1681 cgtttcggcg gcgaatatac gggctattac agctcggaag gcgaatttaa gcgggcattc
     1741 ggagaaaact cgccgacata cagggaacat tgcaacccga gctgcgggct ttatgaaccc
     1801 gtattgaaaa aatacggcaa aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac
     1861 ttcggcgatt atttcatgcc gttcgccagc tattcgcgca cacaccgtat gcccaacatc
     1921 caagaaatgt atttttccca aatcggcgac tccggcgttc acaccgcctt aaaaccagag
     1981 cgcgcaaaca cttggcaatt tggcttcaat acctataaaa aaggactgtt aaaacaagat
     2041 gatacattag gattaaaact ggtcggctac cgcagccgca tcgacaacta catccacaac
     2101 gtttacggga aatggtggga tttgaacggg gatattccga gctgggtcag cagcaccggg
     2161 cttgcctaca ccatccaaca tcgcaatttc aaagacaaag tgcacaaaca cggttttgag
     2221 ttggagctga attacgatta tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa
     2281 agcacgcaac cgaccaactt cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa
     2341 gaccaactca aacaaggtta tgggttgagc agggtttccg ccctgccgcg agattacgga
     2401 cgtttggaag tcggtacgcg ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc
     2461 tatttcggca agagcatccg cgcgacggct gaagaacgct atatcgacgg caccaacggg
     2521 ggaaatacca gcaatgtccg gcaactgggc aagcgttcca tcaaacaaac cgaaaccctt
     2581 gcccgccagc ctttgatttt tgatttttac gccgcttacg agccgaagaa aaaccttatt
     2641 ttccgcgccg aagtcaaaaa tctgttcgac aggcgttata tcgatccgct cgatgcgggc
     2701 aatgatgcgg caacgcagcg ttattacagt tcgttcgacc cgaaagacaa ggacgaagaa
     2761 gtaacgtgta atgctgataa aacgttgtgc aacggcaaat acggcggcac aagcaaaagc
     2821 gtattgacca attttgcacg cggacgcacc tttttgatga cgatgagcta caagttttaa
     2881 aggcagcccg cgttttgtag aaaaccgcaa tgccgtctga aagcccttca gacggcattt
     2941 gtttccccaa acgcatcatc ctgccgcaag cctatgccaa tccgttttat cgcatcggca
     3001 actcaaagaa aaatccattt cattcccacg cagggaagcc ggttgatttc ggttattttt
     3061 ggttgtttcg ggtaatttat ga
//
LOCUS       DAXHRI010000187         2925 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXHRI010000187 DAXHRI010000000
VERSION     DAXHRI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.187"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(111..1421)
                     /locus_tag="ACFM75_001939"
     CDS             complement(111..1421)
                     /locus_tag="ACFM75_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC6256422.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPYVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDGLKKLIAKVLALPEYRDFTIEADAQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKTLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRMAWEDIGLADPRALTIANDAAATYERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVAENSSDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYRPVPRGLEIKIGEKLAWLKSLDEEVLKAK"
     gene            1733..2155
                     /locus_tag="ACFM75_001940"
     CDS             1733..2155
                     /locus_tag="ACFM75_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256423.1"
                     /translation="MEWVSLFSDPQKDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADPQLGYTPELSVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVSDGDGVQDVASGKKYRQTIVP"
     gene            2843..2925
                     /locus_tag="ACFM75_001941"
                     /pseudo
     CDS             2843..2925
                     /locus_tag="ACFM75_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          812 a          712 c          700 g          701 t
ORIGIN      
        1 accttgtctt tattggcttt atcgataaac aggataagac ctgaaaaaag ccgatacgcc
       61 tttttggtgt accggctttg ccatactgtt ctgcttcaga cggcattgct tcattttgcc
      121 tttaatactt cttcgtccag cgatttcaac cacgccagct tttcgccgat tttgatttcc
      181 agcccgcgcg ggacgggtcg gtagaaatcg ggttcgtcca ggccgtcggg catatagctt
      241 tcgccggcgg cgtaggcgtt cggttcgtcg tgggcgtagc ggtattcgcg tccgtagccc
      301 aattccttca tcagcttggt cggggcgttg cgaagatgga caggcacttc gtcgctactg
      361 ttttctgcaa caaatcggcg catttggttg tatgccttgt agcccgcgtt ggatttcgcg
      421 gcggcggcga gatacaacac ggcttgcgct aaagcgagct ccccttctgg cgagcctaag
      481 cgctcataag tagcggcggc atcattggca atcgttaagg cacgcggatc ggccaagccg
      541 atgtcttccc atgccatgcg gacaatgcgg cgggcgagat agcgcgggtc ggtgccgccg
      601 tcgagcatac ggcaaaacca atacagcgcg gcgtttggat gcgaaccgcg cacggatttg
      661 tgcagggcgg agatttggtt gtagaaactc tcgccgcctt tgtcgaaacg gcggatttgc
      721 gccccgagac tgtcggcgag aaattcggcg gtcagggttt tcagacggcg cgtgtcggcg
      781 gcgcgtaaaa gttgttccaa caaattcaac aatctgcgcg catcgccgtc ggcggtattg
      841 acgagcagct cctgcgcgtc ggcttcaatc gtgaaatccc ggtattcggg caaagccaac
      901 actttggcaa tcagtttctt gaggccgtct gaagacaagg attgcaagac ataaacttgc
      961 gccctactga gcaatgctgg attgacttca aacgacgggt tttccgtggt agcgccgata
     1021 aaggttaata agccgctttc gacataaggc aaaaacgcgt cctgctgcgc cttgttgaaa
     1081 cggtggactt catcgacaaa caaaatcgtc gcgcgtccct gttgcaaagc gatttcagcc
     1141 ttatcgattg ccccgcgtat gtccttcacg ccggaaaaca cggcggaaac aggcaaaaac
     1201 tgggcattga aactctgcgc caaaatccgt gccaacgtcg tcttgcccac acccggcggc
     1261 ccccacagca acatagaatg cggcttgccg ccttctaccg ccacgcgcaa aggtttacct
     1321 tcgccgatga ggtgttcctg ccccaccacg tcgtcaagcg tatgcggacg caatcgttcg
     1381 gcaagcggcg cgtcgggttc tcgggcaaac aaatcggtca taacggctcc gtcaacaggt
     1441 tttcaaacaa tatgattata cggcagggaa cggcggcgtg ccgcatacgg attccgcccc
     1501 tccgtttgcc ttaagccgat attaggcgca tactggaaaa gacgagagac ttcacacaat
     1561 atatccggca cggagaccga ttccgcaccg gcatgacaat acccaaatca gcgtttcaat
     1621 taaacattaa ggagactaaa atagaaaatt tgctttatct accattgctt tgttgattca
     1681 atcggcatta tgttttgagg cggaagccca tgaatatact aatattcaag agatggaatg
     1741 ggtgtcttta ttttctgatc cgcaaaaaga cgatgatagt cttataaccc ttaaagatga
     1801 aaaaatcact gtaaaaaact atattgtgcc ttggtggaaa aaaggtgaaa actttagaaa
     1861 attagaactt attaccactg gtaaaaatga taatggaaat ttaacggtaa atggaattta
     1921 cggcatccaa tcttttgcag acccgcaatt aggatatacg cctgaattat cggtgaaaag
     1981 caataatgat attctattta aaaataatct ccaatctgtt gacgtgaagc cccattccgg
     2041 aaatatccat ttggatgcca aaggaaaaat tcaatttaat ttgaatgttt ccgatggtga
     2101 tggggtacaa gatgtagcca gtggaaaaaa ataccgacaa acaatagtgc cttagtgcgg
     2161 tatgtcaaca ctatcagccc cccgatgaaa atgacaaagg cagcatatcc tttcattcaa
     2221 aaacaggcaa tttgttcaat atgaacgtta acgttcataa agacacacac catttcggta
     2281 ttcacaacct caacaatacc gatgttcatc tgactgccga agacagcgat aatgtgattg
     2341 agattaaagg ggaactcgcc gaccctatgc cgacaggaaa tgttgagagt acaagttttt
     2401 taaggggcat tgaaacggct tttaaatcca ataccgtatt aacggggaat aacaataaaa
     2461 tatccatcgc cactgagggc aagggcttgg gattgggcgt aaatggtctc cagtccaact
     2521 ccccctattc cggacacacg acgggcattt tcatcaataa tcaatcaagc gaccttaccc
     2581 aaccttccct tgatggcatc agcaccatca aactcaaagc cgtcaaggga aataatgaag
     2641 ttgatttaaa tgtaaaaaac cacgcctccg taaaaggcat tatcgcgtcg cattctgcaa
     2701 aagcaacgct tgaagccgaa ggggacaata ttgtccaggt taaaaatccg gacaccaata
     2761 cggctttaat cgatgctttg aaaaaacaat accccaatgc ttcgaaagaa ctatacaggg
     2821 tgggggttca atccacgact ggaacagtac gcatttcgcc aaacggcaaa atcgcattaa
     2881 cggaattgaa aacttttgga attgtacaaa acggcatttg cgtca
//
LOCUS       DAXHRI010000188         2925 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXHRI010000188 DAXHRI010000000
VERSION     DAXHRI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.188"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            31..1248
                     /locus_tag="ACFM75_001942"
     CDS             31..1248
                     /locus_tag="ACFM75_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6256424.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTAIAIIGLANIAGCVF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            1245..1376
                     /locus_tag="ACFM75_001943"
     CDS             1245..1376
                     /locus_tag="ACFM75_001943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256425.1"
                     /translation="MKECRLKTLRRHFPNTPPVSGLHDTQISLIKPQTTAAIKPPFK"
     gene            complement(1451..1591)
                     /locus_tag="ACFM75_001944"
     CDS             complement(1451..1591)
                     /locus_tag="ACFM75_001944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256426.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAVSDGI
                     LS"
     gene            1604..2735
                     /locus_tag="ACFM75_001945"
                     /pseudo
     CDS             1604..2735
                     /locus_tag="ACFM75_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          683 a          866 c          695 g          681 t
ORIGIN      
        1 cgcaaactta accgactcaa aatacacatt atgacgcaca ccgcatcgaa aacacccaaa
       61 ttttggctga ccatcaccgc cgccgcattc atcctgctga ttaccatcgg catgaggatg
      121 acgctcggac tgttcgtcca acccgtcgtc aacaccaccg aattgagcat tgcccaattc
      181 agcctcatca tcgccgtttt ccaactgatg tggggcatat cgcaaccatt gtccggcgcg
      241 cttgccgacc gtttcggcgc gttcagggta ttaagcggcg gcgccgtcct gctcgtctgc
      301 gcctgcctga ttgcccccaa catcccgact tattgggggc tgatgattgc cgtcgggctg
      361 ctgctcgcat tcggcaccgg ttccggcggt ttctccatca ttatggggca ggtcgccgcc
      421 caagtccccg cacacaaacg cggcttggca tccggactgg tcaacgccgg cggttcggca
      481 ggacaattcc tgttcgcacc gctggttcaa ggactcgtcg tcctgcccga agtcggctgg
      541 acgggcacat tttacgtttg gggcgcaatc gccctgctga ttctgcctgt ctcatggtgg
      601 cttgccggcg gcaacaacgc cgcccacacc caacacgccc aagccacaca cggacaaagc
      661 ctcggagaag cagtcaaaac cgccttcaaa accccaagct acatcctgct gcacctaagc
      721 ttttttgcct gcggcttcca catcgccttt ctcgtaaccc acctacccac ggaagtcgcc
      781 ctgtgcggac tgcccgccac cgtcgcatcc acagccatcg ccatcatcgg actggcaaac
      841 atcgccggtt gtgtgttctc aggctggtgc acaagccgcg ttcggggcaa atacgtcctg
      901 ttcggactgt acgcctcgcg cgccgcgatg atactcatct acattttttc gcccaagacc
      961 gacctcaact tctacatttt cgccgccgca ctcggattta cctggctcgc caccgtcacg
     1021 ccgaccgcct ccattaccgg caaactcttc ggcacacgct acctcgccac cctgttcgga
     1081 ctgacgatgc tcagccacca aatcggcgga ttcctcggct cgtacatcgg cggcatcgtg
     1141 attacacaat tcggcgacta cggctggatg tggtacgccg acgcactgct ggcgggaacc
     1201 gccgccctcc tcaacctccc catccgcgaa ccccgaaccg ccgcatgaaa gaatgccgtc
     1261 tgaaaaccct cagacggcat tttccaaaca ccccgcccgt ttccggactg cacgacacgc
     1321 aaatcagcct tatcaaaccg caaacaaccg ccgcgattaa accgccattc aaataagtcc
     1381 gttttccctt cgggtgtaaa atcatcacaa aacgcgatac tgtatatcgc cgggcgttat
     1441 gcagcggatt tcaggataaa atgccgtctg aaaccgcccc tgcccgggcg ggcgaagcgt
     1501 cggatgcggt gcgcttggca aaggtttgcc cgccgcgccc cgtatccggc atttcatcaa
     1561 tgcagaccgc atttccaatc ctataattca aggtgtccgc cttatgaaaa tcgtttttat
     1621 cacaacagtc gcatccagca tttacggttt ccgcgccccc gtcattaaaa aattaatcgg
     1681 caaaaaccat caggtgtatg cctttgtatc ggagttttcc gacaatgaat tggatattat
     1741 cagggaaatg ggggttacac ccgttaccta ccgttcaaac cgcagcgggc tgaacccgtt
     1801 ttccgatata aaatccacct tcctcatctt taaagaactc aaaaaaatat cgccggattt
     1861 ggttttccct tatttcgcaa aacccgtgat tttcggcact tttgccgcaa aactggcagg
     1921 cgtgcccaga atcgtcggga tgctggaagg tttgggattc gcatttaccc cgcagccgga
     1981 aggcataccg ttaaaaacaa aaatcataaa ggggattttg attgccttgt accacattgc
     2041 cctgccgatg ttggaaagcc tgattgtatt aaaccccgac gacaaagacg aactgacgga
     2101 caaatacggc atcaaaataa aaaacatcca tattttgggc ggaatcggtc tggatttgcg
     2161 gcaatatcct tattccgagg cggatattcc cgatgaaaaa gaacccgtaa aatttctctt
     2221 tatcggcaga ttgttgaaag aaaaagggat tgatgagttt attcgggcgg cggaacaggt
     2281 taaggacaaa taccccgata cggtttttac cgctttgggc gcaatcgaca aatcacgcgg
     2341 gggggggggg cgatttggaa cgccttatcg cccgcgatat tatccgtttc cccggttttg
     2401 tgaacaatgt ttccgaagtg ataaaggcgc atcatatatt cgtattgccg tcttattata
     2461 gggaaggcgt tccccgaagc actcaggagg caatggccgt cggcagggca gtgattacga
     2521 cggatgtccc cggatgcagg gaaacggtcg ccgacaaggt caacggcttc ctgatcgaac
     2581 cttggaatcc ccgcatcttg gccgaaaaaa tgatttattt tatcgaaaac agggaagccg
     2641 tccgcctgat ggggaatgca agttatgcga ttgccaaaga taaattcgat gccgaaaaag
     2701 tcgatttgaa attgctcgat attttgaagg cgtaaacaag gctgcccgct tttgagtttc
     2761 ggtcatttct gataaacccc cgtcattccc gccacacccc cgtcattccc tcgaaagcgg
     2821 gaattcaggt tcgtttagtt tcggtcattt ccgataaatt cttgcaactt tgcgtttcta
     2881 gattcccact ttcgtgggaa tgacggcgga gggttgctgt tttcc
//
LOCUS       DAXHRI010000189         2922 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXHRI010000189 DAXHRI010000000
VERSION     DAXHRI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.189"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(121..1572)
                     /locus_tag="ACFM75_001946"
     CDS             complement(121..1572)
                     /locus_tag="ACFM75_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6256427.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFSNVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1595..2215)
                     /locus_tag="ACFM75_001947"
     CDS             complement(1595..2215)
                     /locus_tag="ACFM75_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6256428.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPESIIPLFCIAEASIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            complement(2414..2790)
                     /locus_tag="ACFM75_001948"
                     /pseudo
     CDS             complement(2414..2790)
                     /locus_tag="ACFM75_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          910 a          669 c          647 g          696 t
ORIGIN      
        1 taggaataag aattcgggac aaaatgttta aaccattttg tcccgactgc tgtgatgcgg
       61 tttttttgaa ctagcaggac gttacctaaa acctgcgttg cctgaaaccc tttaagacgg
      121 ttaaatccct ctgccgtatt tgtattctac aatcatatta tcgtcggtaa tgacttccgc
      181 cccagccgaa ggttccgtca tctgaatcag catacgagag acaacctttt gtgctgcagc
      241 atccacggtg ctgctgtcaa atacgtgcct gccgctttcc ggccaaatca accgggagag
      301 acgttgcttg agcagttctt tattagggaa aactaccggg gttgccgagc cgactaccat
      361 atgcccatag cggtatgcat agggaatact gtgtacggcg gtagcaaaag catgcgggct
      421 gtgcgtggta ttaaacatta caataccatc cggggtaagg tggctttgca cctgttttaa
      481 aaattccgca ctcaacaggt tggtggaata ggcacgccag taccaagtcg tattcatcaa
      541 aatcaggtcg aatttttcat caggatggcg acgcagccat ttcctaccgt catccaatac
      601 aatttcaaca cgtttgtcct gcaaaagcgg ggcgatttgc ggctcatccg cgataaggct
      661 acggtatgcc ggattgattt ccgcaacgat catcgactgc atttccggaa tggcagacaa
      721 gacgcgcgcc cacgaacctg tactcaatcc aacgacgaaa atgcggcgta tgccagactt
      781 cagggagggt agcagatagg cacgttcgat gccgttgaca ctattgaata catcggtatt
      841 gtatgcgccg tcgtatacat tcgccccata aacaacctta tcaccatctc tatggtaaac
      901 cgcaacaatg ccgtgtttgt tttcaatcag cctatccgga cggtcagcaa tattttgaaa
      961 gacagaatcc ggcagtagga acatgaggat gccgaacatt agggaaactg ctaccgacac
     1021 tgcattcagt cggagacttt tttggaacag tgtacaaaac aaagggacag cagcagaaat
     1081 caaacagatg agcaggtaaa tctgttgggt ggacaagaaa tcaagtatca caaagccgat
     1141 aaggaccgga cccaatgcac tgccggcaac gttggagaaa taaacattgg aaacctgtcg
     1201 tccggatttg ttgccatccg tacccacatg gtgtacgagc gggaaaatca accctctgac
     1261 gacggcagac agggtaatga agataccggc gtggtggacg aagccggaaa aacccgtcaa
     1321 taaccacgca gcacccaaaa tcaaaaagtc ggcaataccc gcccacaaga agcactgccc
     1381 gataaaggga atatcaacaa agcggctgcg gcaaatccgt ttgccaaaat acgcgccgac
     1441 ggcgataccg gtcagaaaac aggcaagggt aaatgaaaat gcctgaggca cggactgtgc
     1501 tgcgaacgaa aacatcctta cccacaagac ttctataccc aagctcaata agccgctaag
     1561 gaaagaaagc atataaatca aactagtatt cggtttagtg ttcactatat ccatcctttc
     1621 tgtaacgcag ccatactgaa gcagcaatca gaaggttaaa gcaggctgtc agcgcaatgg
     1681 tttgggagag ggtaaaaaag acgtagaaaa attcggcggc ggcaagcgat ccgagtgccg
     1741 cacccaaagt gttgaaaaaa tataaggtac cgatagactc gccaacatta tgtattttcc
     1801 ggttaaaaaa acaggtcagc aagggcaagg tcgcgcccat cataaaggta ggaagcagca
     1861 ataagaggaa attggcagca gcgatgatgg gcaaatcagc ctcaactaaa agatgcccca
     1921 agccggaaat cagacccctg cttaccaaac cgaacagacc gatggatgct tcagcgatgc
     1981 aaaacagagg gatgatactt tcaggaaaac ggtcagcaat gcgtccaccg aaatacgcac
     2041 ctacacccaa gccgaccata aatacagaaa taatgacagt aatcgaactc aaatcgatac
     2101 ctatgtgact gaatagaagc ctctgccagc tgacctggta aatcagggcg cagaatccag
     2161 aggcgaaaaa caccagcgac aatcctttca gggttttact tgcggtcgaa ttcatatttt
     2221 ctattttcaa ataggcggaa ctataaatga aatgtatcaa tgtgtaaaca tttgtattaa
     2281 ccgtaggaaa tcaccgtatt gctcaaactg caggccggga aaataaactc gtaagcccgc
     2341 aaacggcgta tcactgttca aactgctgtg cggcttcacg gtgttataaa aataatgggg
     2401 ctgtcctgga caactaagat aaactcgatt ttactaattg ttttaaaatg gaacaagaac
     2461 ttttatctca ctgttgttaa aacgccattc gcactccttt aaatacagct caaaatgcgc
     2521 tttgggaatg ccgttaaact tgcgtaaatg acgttttgcc cggttccaaa aattctcaat
     2581 tccattgata tggttttgtc gttcagcaaa atgtgtgctg tgattgatac ggaaatggct
     2641 aaattcgctc acatctaata catcatagct acgataacaa tccgtataaa caatgctgtc
     2701 aggctctgtt gctgttcttg cagttacacc tgcgacaaat agctcaatga gtttattttg
     2761 tttataccgg cttagacgac tttttctcat agggataatt ctaacttaat ttgaatttcc
     2821 ctatttatct aggacagccc taatgattaa attggattta aaaagcataa atttatatga
     2881 tattgatttt gaaaaattta cccccgaaat tccagataat tt
//
LOCUS       DAXHRI010000190         2900 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXHRI010000190 DAXHRI010000000
VERSION     DAXHRI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.190"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(106..181)
                     /locus_tag="ACFM75_001949"
     tRNA            complement(106..181)
                     /locus_tag="ACFM75_001949"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Phe,seq:gaa)
     gene            complement(372..1106)
                     /gene="lptB"
                     /locus_tag="ACFM75_001950"
     CDS             complement(372..1106)
                     /gene="lptB"
                     /locus_tag="ACFM75_001950"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC6256429.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(1180..1710)
                     /gene="lptA"
                     /locus_tag="ACFM75_001951"
     CDS             complement(1180..1710)
                     /gene="lptA"
                     /locus_tag="ACFM75_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC6256430.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(1691..2272)
                     /gene="lptC"
                     /locus_tag="ACFM75_001952"
     CDS             complement(1691..2272)
                     /gene="lptC"
                     /locus_tag="ACFM75_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC6256431.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(2269..2805)
                     /locus_tag="ACFM75_001953"
     CDS             complement(2269..2805)
                     /locus_tag="ACFM75_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC6256432.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT          636 a          744 c          758 g          762 t
ORIGIN      
        1 ctgcccgaca gccgcaaaga ccgtatcata catagcaggc attgcggtgc gggattactg
       61 ccgcaaacgg caggcaaaaa aaataaccgc ttcaaggcgg ttttttggtg gccagaggcg
      121 gaatcgaacc gccgacacac ggattttcaa tccgttgctc taccaactga gctatctggc
      181 ctttgtctgt ctcgttaaga gatgcgtatt aaaccaaatt acgggacgac atgcaagcat
      241 atcgggcaat attctttgaa aacaggttta agtcaatgtt ttaaatttga attaatttat
      301 gtaaaaacaa cataaatccg tatttttgcc gcctgcccga cgatattagg tcgcccgtct
      361 gaaaaatatt ttcaatattt gaagttctta cccagataaa cagaacgaac ctgttcgttt
      421 ccgaccaaat catcaggttt tcccgatgcc agcaccgcac cgtctgaaat gatgtaggcg
      481 cggtcgcaga tgctgagggt ttcgcgtacg ttgtggtcgg taatcagtac gccgataccg
      541 cgcgacttga gaaatccgat gattttttgg atgtcgatga cggcgatggg atcaacaccg
      601 gcaaaaggct catccagcaa aataaaatgc ggtttcatgg cgagtacgcg ggcgatttcg
      661 acgcgccgcc gttcgccgcc ggacagcgac ggcgcggggc tgcggcgcaa gtgtccgata
      721 ttaaggtcgg cgagcagttt ttcgatttcc ctgtcgattt gatttttatc tttggttctg
      781 atttccaaga tggcgcggat gttttgttcg acggtcattt tgcggaatat cgaggcttcc
      841 tgcggcaggt agccgacacc gaggcgggcg cgttcgtgta tgggcaggtg gcgcaattct
      901 tgtccgtcta gggttacgct gcctgcgtcg gcggcaatga gtccgactat catgtagaag
      961 ctggtggttt tacccgcgcc gttgggcccg agcaggccga cgacttctcc gctttcgatt
     1021 tcgagggaga agcttttaac gacttggcgt tttttgaaac ttttttgcag gttttgaaca
     1081 acaaggcggc tgacgtttgc gctcataaat atctcttcaa tatcttcggt cggcaaatgc
     1141 cgtccgaacc aggtttatgt ttcagacggc attttgggat tattcggatt tttgcgtact
     1201 cgaaggctgg ataacgacgc tgaccctgcc ggatttggaa gcggattttg cgccggattt
     1261 tgtgctgccg ctgatggtat agacttcggt tttggtgttg tatgtaatca ccgcaccttc
     1321 ggcgacatcg ccgccgcgct gtactttggc attaccggtt aagactacgg tgctgcctgc
     1381 agatgaataa gcaacgttgt ttgcctgtcc gcgcaccgtg cctttgccgc cgtccaatgt
     1441 ctggctgaag cggactggcg aaccttccgc cctcacggat tcgccgcctt tgccgccgcg
     1501 tgtaacattg acgcgggcgg cggaaatatt gagcgtaccc tgtctgatga cgacgtttcc
     1561 gctgaatgtg gtgctttggt tggcttgatc gagcgaacct tggtcggcct caatctgaat
     1621 aggctgcctg ctgtcgcttt gaagggcaaa agcgggggac gccgaaaaaa atgctattaa
     1681 aacaaatagc ttacatatct tttgtatcat aaatcgtggc tttcacttta gatgagaagt
     1741 tcaacatgcc tgttttgtgg tcgtaagtca tgccgcccgc ctgaccgtgc gatgcaccat
     1801 attggaaact gacaggcgta tcggtttggg catattgaga ttcggtatcg acgtgcagct
     1861 tttcggcttc aactttaccc gcctgccgtt tgccgtcggc ggttttggtc agcacaacgt
     1921 tgtttttaaa aagaacctgt ttgttttcgg tatggtaaac ggcttcatcg ctgccgactt
     1981 cgtacaacaa cctgccttct tggaagaaca cgagatgcgg tgagtcgaaa tgaatgtcgc
     2041 tgctttcggg aaactgtttc gcgcccttcg aactcaaatg ttctttcaag tatccctgtt
     2101 cgtcaaaccg cctgccatcc aatccgtcca ttgtgtattg cggttcgtcg ggattgagcc
     2161 tgacttcttc aatctcgact tcgctgatgc gtcccaacca tgccgacagg ctgcccaagg
     2221 caaccgccaa tatcaatggg aacgcaattc cgtaccgcca tcttactttc atttgatgta
     2281 ctcgttcaaa gccgcgccca aagtcccttg cgcctgcata atcaggtcgc acacttcgcg
     2341 caccgcgccc gcaccgccgg ggcgtttggc aatataggcg gcgtgttgca gcgtaaacca
     2401 atgcgcgtcg gggacggcaa ccggcaatcc gcagcgtacc ataacgggca aatcgaccac
     2461 gtcgtcgccg acaaaggcac actcggcttc ttccacgccg acctgagcgc gcagctcttc
     2521 atacgcggcg cgtttgtccg aaatgccttt gaagtagtaa tttatgccca actgtttgac
     2581 gcggatgccg acggagggcg cgtcccgacc tgtgataatc gcagtctgca cgccgcttgc
     2641 ctgaagcatt ttcagaccgt gtccgtccag tgtgtgaaac gatttgattt cttcgccgtt
     2701 atcgcggata aagatgcgcc cgtcggtcaa aacaccgtcc acatccaaga tcagcagttt
     2761 gattttggcg gcgcgcgcct gtaattcggg agaaattgcc tgcatgggat ttccttgttt
     2821 tcagacggca tatcttacca caaatgccgt ctgaaacggc tgcaacgcct tatatagtgg
     2881 attaacaaaa atcaggacaa
//
LOCUS       DAXHRI010000191         2889 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXHRI010000191 DAXHRI010000000
VERSION     DAXHRI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.191"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            28..2178
                     /gene="dinG"
                     /locus_tag="ACFM75_001954"
     CDS             28..2178
                     /gene="dinG"
                     /locus_tag="ACFM75_001954"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC6256433.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            2215..2828
                     /locus_tag="ACFM75_001955"
                     /pseudo
     CDS             2215..2828
                     /locus_tag="ACFM75_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          711 a          944 c          738 g          496 t
ORIGIN      
        1 cccacttcta ccgccgtaca aaacgccatg ctcaccgatt tagaaaaaaa cgccatccgc
       61 gaccattacc aaaacatcgg caaaaacctg cccggtttcc gtccgcgcgc ttcgcagcgg
      121 gaaatgattg cggcggttgc caacgctttt tcgcggacgt tggcgcgcga agaaggcggc
      181 gagccgccca agcgcgaggg cgagagcatc gccgtaatcg aagggccgac cggcgtgggc
      241 aaatcgcttg cctacctgct ggcgggcggc atcatggcgc agacgcgcgg caagcggctg
      301 attgtgagca gcgcaacggt tgccttgcag gagcagttgg tggaccgcga cctgccgttt
      361 ttggtcgaaa aaagcggttt ggaactgagc ttcgcgcttg ccaaagggcg cggccgctat
      421 ctctgcccct acaaactcta tcaactgacg caaagcaatg cccagcaaaa cctgctcggc
      481 tttgaagccc ccgccgtctt gtgggacagc aaacccaagc ccgaagaatt gaagctgctg
      541 cgcgacattg ccgacgaatt ttctgcccga cggttcaatg gcgaccgcga cgcctggccg
      601 gaaaaaatcg acgacgcgat ttggctcaaa gtgaccaacg accgccacgg ctgcctgaaa
      661 accgcctgtc ccaaccgtcc ggaatgtccg ttttacctgg cacgcgatgt cttggaaacc
      721 gtcgatgtcg tcgttgccaa ccacgatctt ctgcttgccg acatcagtat gggcggcggc
      781 gtgattctgc ctgcgcccga aaacagtttc tattgcatag acgaagcgca ccacctgccc
      841 aaaaaagccc tcagccgttt tgccgccgaa cattcatgga atattgccgt ttggacgctg
      901 gaaaaactgc cgcagctgac cggcaaaatt gccgcgctta ccgataaagc cgaacttgcc
      961 aacctggccg acgaagccgc cgcatccttg ctcgacagcc tgcacgaatg gcagttccat
     1021 ttggcggaag agccgtcttt aagtatgggg ccgtctgaaa acgacagacg aaccaacagc
     1081 gaaccgactt ggctgtggga agacggcaaa atccccgaag gcctcgaaac caccgtttcc
     1141 aatacggcca ttgctgcgcg cagcctgctc aaacacgttg tcgggctgaa cgatgcgctt
     1201 tccgccgcgc gccgcgaaaa agaacaggac ggcgcgctcc tcgaccgcct gaccggcgag
     1261 ttcggcatct tcatcgtccg tatcgaacaa atcagcgcgg tttgggattt gctctccacc
     1321 gtccccctcg aaggcgaaga gccgctggca aaatggataa cccgccgtgc cgacgacaaa
     1381 aacgactaca ttttcaacgc cagccccatc agcagcgcat cccaccttgc caacagcctg
     1441 tggcggcgtg cggcaggcgc ggtattgact tccgccaccc tgcaatcctt gggcaacttc
     1501 aacctgatgc tgcgccaaac cgggctgcaa tggctgcccg aaaccaccac cctcgccctc
     1561 aaaagcccct ttgactttga aaaacagggc gaactctaca tcccccccat atacgccagc
     1621 cccaaagacc ccgaagccca caccgccgcc gtcatcgaat ggctgcccaa gcttatttcg
     1681 cccaccgaag ccatcggcac gctcgtcttg ttttcctcgc gcaaacaaat gcaggatgtc
     1741 gccctgcgcc tgcccggaga ctacctgccg ctcttgctcg tacaaggcga attacccaaa
     1801 gccgtcctcc tgcaaaaaca ccaccaggcc atagaagaag gcaaagccag catcatcttc
     1861 ggactcgaca gcttcgccga aggactcgac ctgcccggca ccgcctgcgt gcaagtcatc
     1921 atcgccaaac ttcccttcgc catgcccgac aaccccatcg aaaaaaccca aaaccgctgg
     1981 atagaacagc gcggcggcaa ccccttcatc gaaatcaccg tccccgaagc cggcatcaaa
     2041 ctcatccagg ccgtcggccg cctcatccgc accgaacaag actacggccg cgtaaccatc
     2101 ctcgacaacc gcatcaaaac acagcggtac ggccaacaat tattggccgg cctgccgccg
     2161 tttaaaagga tagggtaaac ataccgccct tcccgcaaca gaaaggaaaa attcatgaac
     2221 tttacccgac tgctcaacca agtcttaagc acggttcaaa aaaaggcaac acattctccg
     2281 acagcccgct caattcattc ggcggaggcg cgctggttgc cggtgtcgcc tccatgctgc
     2341 tgaacggtaa aaaccgcaaa accatcacca aaatcggttc gaccgccgct ttgggctacc
     2401 tcgcctaccg gggctatcag atgtggcagc aaaacaaagg gcgggcaacc gtaacacaaa
     2461 gcgatttcca acctgccgga gaaaccgaag aaacatacag ccgcaccgta ttgcgcacca
     2521 tgatagccac cgccgcttca gacggcatga tagacgaagc cgaacgccgg actatcgaac
     2581 aggaaagcgg cacagacccc gaaactgctg catggctcgc cgccgaatac cgcctgcccg
     2641 caagcatcga agacatcgcc gccgccgtcg gcaacgatga ggcgttggcg gcggaggcct
     2701 atttggcggc aagactggta tgtgccgatt tgtcgcggaa agaaaccgtc ttcctcgccc
     2761 gcctgtcgca ggctttggaa ctggatgaca atctggtgga gagtttggaa aggcaattgg
     2821 ggttttgatg acacagccgg ggagggaaaa tcatcaaaaa acggttattc cgaccccggc
     2881 ctcaaaacg
//
LOCUS       DAXHRI010000192         2888 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXHRI010000192 DAXHRI010000000
VERSION     DAXHRI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.192"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(117..1106)
                     /gene="pdxA"
                     /locus_tag="ACFM75_001956"
     CDS             complement(117..1106)
                     /gene="pdxA"
                     /locus_tag="ACFM75_001956"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC6256434.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGQGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1266..2693
                     /locus_tag="ACFM75_001957"
     CDS             1266..2693
                     /locus_tag="ACFM75_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6256435.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          573 a          788 c          830 g          697 t
ORIGIN      
        1 ctggattccc gcctgcgcgg gaatgacgat ttggaaatta cccgaaaccc aaaaacaact
       61 gaaaccgaac agaccggatt cccgcctgcg cggaaatgac gggtctttta tcatctttaa
      121 aggctgccgc gcgccatctc gacggcggtc tccacggcag ttatcaggct gccggaatcc
      181 gccctgccgg ttgccgccaa atcgagcgcg gtgccgtgat cgacggaggt gcggataaag
      241 ggcagcccca gtgtgatgtt cacgccctgt ccgaagctgt ggtatttcaa cacgggcagg
      301 ccttggtcgt ggtacatcgc caatacggca tccgcacctt cgagcataaa cggttggaac
      361 aaggtatccg ccggatacgg gccggcgagg tttatccctt cgcggcgcag gttttcgagc
      421 gcggggataa tggtgtcggt ttcttcgtgt ccgaggtgtc cgccttcgcc ggcgtggggg
      481 ttgagtccgg cgactaggat tttgggattt ttgatgccga atttgtgttt taagtcgtga
      541 tgcaaaatgc gtgcgacgct ttcaatcagc ggttgcgtga tggcggcggc aacgtctttc
      601 agcggcaggt gggtcgttac gagggcgacg cgcaggcctt ggccggcaag catcatcaca
      661 acctgccccg tgccgctttt ttccgccaga tattcggtgt gtccgctgaa aaaacctgtg
      721 cttgcgcgcg cgtcgttgat gatgcctttg tgcagcggcg cggtaacgat gccgtcgaaa
      781 atgccgtctg aaatgcctgc gagcgcggtg tccaaaagtt gcagcacata ggcggcgttg
      841 gcgggattga gtttgcccgc ctcaaccgct tcgacggcag ggatgtgcag cacttccagc
      901 tcgccgtatg ccgcgccgcc tgattccgga tcgaagtcgc gcaagacgac gcttttgccc
      961 aaggcttcgg cgcgcgcgcg caacaggtgt ttgtcgccca ataccgcgca gcggcagggc
     1021 aggcgtgcaa acgccaagtc caaacaaata tcggggccga tgccggcagg ctcgccggaa
     1081 gtaacggcaa aaacaggctg tttcatcgtg tttgctccaa acaaaatgcg attctaacgc
     1141 cgcagccgcg cggcgatgta aatttttctg attttgttga caatctgcta gaatgggcgt
     1201 ttacaaaatt taaaccctgc ttgcataccg ccaatatgtg cgggtttcaa ctttaaggaa
     1261 gcgatatgaa cgagaacttt accgaatggc tgcacggctg ggtcggcgcc atcaacgatc
     1321 cgatgtggtc atacttggtt tatatgcttt tgggtacggg gcttttcttc accgtaacca
     1381 cgggctttgt ccaattccgc ctgttcgggc gcagcatcaa agaaatgctc ggcggccgca
     1441 aacaggggga cgaccctcac ggcatcacgc cgtttcaggc atttgtaacc ggccttgcca
     1501 gccgcgtggg cgtgggcaat atcgcgggcg tggccatcgc catcaaagtc ggcggaccgg
     1561 gcgcggtgtt ttggatgtgg gtaaccgcct taatcggtat gagttcggcg tttgtcgaat
     1621 cttcgctggc gcagctcttt aaagtccgcg actacgacaa ccaccatttc cggggcggcc
     1681 ctgcctacta catcactcaa gggctggggc agaaatggct gggcgtgttg ttcgccctga
     1741 gcctgatttt ctgtttcggc tttgtgtttg aagcggttca gaccaatacc attgccgata
     1801 ccgtcaaagc ggcgtggggt tgggagcctc attatgtcgg cgtcgccctg gtgattttaa
     1861 ccgcgccgat tatcttcggc ggcatcaggc gcatatctaa agcggcggaa atcgtcgtcc
     1921 ccctgatggc ggttttgtac ctctttatcg cgcttttcat cattttgacc aatattccga
     1981 tgattccgga cgtgttcggt cagatttttt cgggcgcgtt caaattcgac gcggcagcag
     2041 gcggcttact cggcggtctg atttcgcaaa cgatgatgat gggcatcaaa cgcggcctgt
     2101 attccaacga ggcgggtatg ggttccgcgc cgaacgccgc cgccgccgcc gaagtgaaac
     2161 accctgtttc gcaaggtatg attcaaatgc tgggcgtgtt tgtcgatacc atcatcgttt
     2221 gttcttgcac cgccttcatc atcttgattt accaacagcc ttacggcgat ttgagcggtg
     2281 cggcgctgac gcaggcggcg attgtcagcc aagtggggca atggggcgcg ggcttcctcg
     2341 ccgtcatcct gtttatgttt gccttttcca ccgttatcgg caactatgcc tatgccgagt
     2401 ccaacgtcca attcatcaaa agccattggc tgattaccgc cgttttccgt atgctggttt
     2461 tggcgtgggt ctatttcggc gcggttgcca atgtgccttt ggtctgggat atggcggata
     2521 tggcgatggg cattatggcg tggatcaacc ttgtcgccat cctgctgctc tcgcccttgg
     2581 cgtttatgct gctgcgcgat tacaccgcca agctgaaaat gggcaaagac cccgagttca
     2641 aactttccga acatccgggc ctgaaacgcc gtatcaaatc cgacgtttgg taaatcccgc
     2701 ccttaccaga gccgcttccc cgcgaagcgg cttttccctt tccgcacact gtaaaaacag
     2761 ggcgaacaag cgtacaatcc caacccttta cttttgaatc catttcgttt ttcagacggc
     2821 atattgaata tagtggttta acaaaaatcg ggacaaggcg gcgaagccgc agacagtaca
     2881 gatagtac
//
LOCUS       DAXHRI010000193         2856 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXHRI010000193 DAXHRI010000000
VERSION     DAXHRI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.193"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>2856
                     /locus_tag="ACFM75_001958"
     CDS             <1..>2856
                     /locus_tag="ACFM75_001958"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6256436.1"
                     /translation="VLKAGRDIQLEAASNRHFDAETHEYKKSGLSGGFRDGVLSVGYT
                     KASTKSNQDQTVTTLTNSQVGSKQGNTVVVAERTLNTKAAILASGSDMHLQGRDVALN
                     AGYTETNNNNDIEQKQSGLSMGITLNPIDAAKANYKRNMQGSGYSNSIVGKTLQRADA
                     GAKASTAAFTPIVINGGRQKTTENRHQEDTQAVVTAVSAQGNLNIVADGGSIRSEGTK
                     LTAEGDILLSATESITLDVARNTADQHGSRKRSGFSIDNRELIPAGTFNDRGNAQGNL
                     DKAIGTQLSAGGMAVLQAQKADINIFGSSVAAQDDTTLSAGRNINIRSSQNNQTKSER
                     QITSGIGTAVISDTEHFAGWMKNRKDSNSNQVEQIKSQVGSLGGNVNMQAGGNYTQQI
                     ANVVAAKDINITAKSVEILADHNRNNSHQSERDVKIGTFAKVSSPLIDLVNAAEGAVK
                     SKADDRTQALQTLAAGAQAYSTYNTATSGGALAKAEVGFGFKTANSSQDQSQNHSQAN
                     VLNAGGNLNIRSTEGNIHLQNTQAQAAETLRLDSAKDLILESGQSSQSADGKNSSLGA
                     SVGVGVSVGAQTGVYAYGEVGGSKGKNHYLAQTHDHTTLQAKKIELASKGDTTLKGAT
                     ATASRIDADVKGRLNIESVQDHTEQDNKQTGAGVRVQVSLGTAWEASGNFNQSKASGS
                     SDSAAIQSGLFAGEGGYHIEADSIHLKGGAITSTAPKEQNELTAKRLTFENIQNQSSY
                     SASNVSLSGSYGSDSPSETPDNADFRQTNLGKAFARSAGKNGTDFNPGLPQYEKGGDN
                     STTYATLSEGRLNIGGKETTTQELGINSDSSNAHRAVAALPDLAKITEKQQIIAKATS
                     DIVSAAHTFSRNRQKTAAENKAKAEAEFEGRLKAQNDGSYEAYAKLDETERQKILINY
                     SETYRKANAEAQNWGVGGKHSRALNAGITLTTGLLGG"
BASE COUNT          899 a          749 c          663 g          545 t
ORIGIN      
        1 tgttttaaaa gcaggtagag acatccagtt agaagcagct tctaatcgcc attttgatgc
       61 tgaaacacac gaatataaaa aatcgggtct gagcggaggt ttccgcgatg gtgttttaag
      121 tgtcggatac actaaagctt ccacaaaatc caaccaagac caaactgtca ccaccctgac
      181 aaactcccaa gtcggtagta aacaaggtaa taccgtagtt gttgccgaac gaaccctaaa
      241 caccaaagct gcgatactgg catcaggtag cgatatgcac ctacagggac gtgatgttgc
      301 tttaaatgca gggtacacgg aaacaaataa caacaacgat atcgaacaaa aacaatcagg
      361 tctatccatg ggcataaccc tgaatcccat tgatgctgcc aaagctaatt acaaacgcaa
      421 tatgcaaggc agtggctaca gcaatagcat agtgggtaag accttacaac gcgccgatgc
      481 cggagcaaag gcatccacag cagcttttac tcctatcgtc atcaacggtg gtcgccaaaa
      541 aactacggaa aatcgtcatc aagaagatac gcaagcagtg gtaacggccg tttctgcgca
      601 aggcaaccta aacattgttg ccgacggcgg cagtatcaga agtgaaggga caaaactgac
      661 tgccgaaggc gatatcctgc tttcggcaac agaaagtata acgttggatg ttgcccgcaa
      721 cactgccgat caacacggtt cccgtaagcg cagtggcttc agtatagata accgagaatt
      781 gattccggca gggacgttta atgaccgtgg caatgcacag ggcaatctcg ataaagccat
      841 cggcacacaa ttgtccgcgg gtggtatggc cgtattacaa gcgcaaaaag cggatatcaa
      901 catcttcggt agcagcgttg ccgcgcaaga tgatactacc ttgtctgccg gccgtaatat
      961 caatatccgt tccagccaaa acaatcaaac caaatcagaa cgtcaaatca ccagcggcat
     1021 cggcactgcg gtaatttccg ataccgaaca ctttgccggg tggatgaaaa accggaaaga
     1081 tagtaacagt aatcaagttg aacagattaa atcccaagtt gggagcttgg gcggcaacgt
     1141 caatatgcag gctggtggca actataccca acagattgcc aatgtggtgg ccgccaaaga
     1201 catcaatatt acagcaaaaa gcgttgagat tctggctgac cacaatcgca acaacagcca
     1261 ccaatccgag cgcgacgtta aaattggcac ctttgccaag gtatcttctc cactgatcga
     1321 cctggtaaac gctgccgaag gcgcggtgaa gagtaaagca gacgaccgca cgcaggcgct
     1381 gcaaacactg gctgcaggtg cacaagctta ttctacctac aataccgcta ccagcggcgg
     1441 tgcattagcc aaagcagaag taggtttcgg ctttaaaact gccaacagca gccaagacca
     1501 aagccaaaac catagccaag ccaatgtttt gaatgccggt ggtaacctca atatccgttc
     1561 taccgaaggt aacatccacc ttcaaaacac ccaagcacaa gcggctgaaa ccctccgctt
     1621 agattcagcc aaagatttga tactcgaatc tggccaatct tcccaatccg ccgatggaaa
     1681 aaatagcagc cttggtgcat cagtgggtgt aggcgtctcc gtaggcgcgc aaaccggtgt
     1741 ttatgcttat ggcgaagtgg gtggcagcaa aggtaaaaac cattatcttg cccaaacaca
     1801 cgatcacacg accctccaag ctaaaaaaat cgaattggca agcaaaggtg ataccactct
     1861 caaaggggct acagccactg ccagccgcat tgatgccgat gtaaaaggcc gtctgaacat
     1921 cgaaagtgtt caagaccata ccgaacagga caacaaacaa accggcgccg gcgtacgtgt
     1981 acaagtttcc ttgggcaccg cctgggaggc ttcgggcaac ttcaaccaaa gcaaagcttc
     2041 aggcagcagt gacagtgcgg ctatccaatc cggacttttt gcaggagaag gcggctacca
     2101 catcgaggcc gacagcatcc acctaaaagg cggagccatt acctctaccg cgccgaagga
     2161 gcaaaatgaa ttaactgcca aacgtcttac ttttgaaaat attcaaaacc aaagcagtta
     2221 tagcgcatcc aatgtcagct tgagcggtag ttacggtagc gacagcccct ctgaaacccc
     2281 cgacaacgcc gacttccgtc aaacaaactt aggtaaagca tttgcacgct ctgcaggcaa
     2341 aaatggtacc gacttcaacc ctggcttgcc gcaatatgaa aaaggcggcg acaacagcac
     2401 cacctatgct accctgagcg aaggccgtct gaacatcggc ggcaaagaga ccactacgca
     2461 agaactgggt atcaatagtg acagcagtaa cgcacaccgt gctgttgcgg ctctgcccga
     2521 tttagccaag attactgaaa agcaacagat tatagctaaa gcaacttccg atatagtatc
     2581 cgctgcgcac actttcagca ggaaccgcca aaaaaccgct gcagaaaata aagccaaagc
     2641 cgaagcggag ttcgaaggcc gtctgaaagc ccaaaatgac ggtagctatg aagcctacgc
     2701 caaactggac gaaaccgaac ggcaaaaaat tctaatcaac tactcagaaa cctatcgcaa
     2761 agctaatgcc gaagcccaaa attggggggt gggaggtaaa cacagccgtg cccttaatgc
     2821 cggcatcacc ctcacgaccg gtctacttgg tggtca
//
LOCUS       DAXHRI010000194         2829 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXHRI010000194 DAXHRI010000000
VERSION     DAXHRI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.194"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(36..512)
                     /gene="greA"
                     /locus_tag="ACFM75_001959"
     CDS             complement(36..512)
                     /gene="greA"
                     /locus_tag="ACFM75_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC6256437.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            904..2205
                     /gene="aroA"
                     /locus_tag="ACFM75_001960"
     CDS             904..2205
                     /gene="aroA"
                     /locus_tag="ACFM75_001960"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC6256438.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLTDALRIAGANVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADARYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2248..2781)
                     /locus_tag="ACFM75_001961"
     CDS             complement(2248..2781)
                     /locus_tag="ACFM75_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6256439.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPGSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          586 a          787 c          781 g          675 t
ORIGIN      
        1 cctgcgtgtg tcgggtgtat cgaaatcaag ccgaatcaaa tatatcggac ttcgataatg
       61 tcgtattcgc gcacgccgcc cggggcttgg acttccgccg tatccccctc ttccttgccg
      121 attaaggcgc gggcgatggg tgagccgaca tagattttgc cctgtttgat gtcggcttcg
      181 tcttcgccga caatttgata ggtaacgtgt tcttccgttt ccaaatcttc cagcgtaacc
      241 gtcgtaccga acacgatttt gccttcggcg tggatttcgg tcggattgat gatgtgggca
      301 acggaaagtt tgtgttccag ctcggaaatg cggccctcga taaagccttg gcgttctttg
      361 gcggcttcgt attcggcgtt ttcggacaaa tcgccgtgcg aacgggcttc ggcaatcgct
      421 tcgatcactt cgggacgcgc cacgcttttg agctgctgca attcctgttt cagcaattcc
      481 gcaccgcgta cggtcagggg gattttttgc atcggtcttt tttctccata ttccggcaca
      541 ccggtttgcg gcagcaaaca taccgcgtac cgtcttgttt tgtgcgtccg gatattaaaa
      601 taaaaataca agccgcccgg aaaatcggcg gcttgtctgt cgttgaacag cggctattct
      661 accaaattct atgaaattgg caatcgtgcc gcgccgccgg caaacgcgcc atgtccgcaa
      721 caaaagctga aaatatgccg acaaagaaat tttagaaaca aaaaatttaa aaataatcaa
      781 ttttcggcat aaaaaaccac atttacggac tttaaaaccg aaaatgccaa acctgagatt
      841 tttcagacgg catttgcacc agtataatgc aggctgtttt tatccctata ttgacgtttt
      901 gccatgaccg aatccgtccg cctccccgtc gcccgtctca aaccttccac cgtcgccctg
      961 cccggctcca aaagcatcag caaccgcacc ctgctgcttg ccgccttgtc cgacaatgct
     1021 tgcgaaatcc attccctgct caaatccgac gataccgacc gtatgctcga agcactcgat
     1081 aaactcggcg ttgaaatcga atatcttgcc gaagaccgtc tgaaagtgca cggcacgggc
     1141 ggacgcttcc ccaaccgcac tgccgatttg tttttgggca acgcgggcac ggcgttccgc
     1201 ccgctgaccg ccgcgcttgc cgttttgggc ggcgattatc atctgcacgg cgtgcctcgt
     1261 atgcacgaac gccccatcgg cgatttgacc gatgcactgc ggattgccgg ggccaatgtc
     1321 gaatatctcg gcaaggaaca ctatccgccg cttcatatcg gcgaacgcca agacaacggc
     1381 gagcgcgtga ttccgattaa aggcaatgtg tccagccagt ttctgaccgc ccttttaatg
     1441 gcgttgccgc tgaccgggca ggcgtttgaa atccgtatgg tcggcgagtt aatttccaag
     1501 ccctatatcg acattacttt aaaactgatg gcgcaattcg gcgtacaggt tatcaatgaa
     1561 ggctaccgcg tcttcaaaat tcccgccgat gcgcgctacc acgcgcccga acacttgcac
     1621 gtcgaaggcg atgcctccag cgcgtcctac ttcctcgcag ccggtttgat tgccgccacg
     1681 cccgtccgcg ttaccggtat cggcgcaaac agcatacagg gcgatgtcgc ctttgcccgc
     1741 gagctggaaa aaatcggtgc ggacgtggtt tggggcgaaa acttcgtcga agtctcgcgc
     1801 ccgaaagaac gtgccgtcca agcctttgat ttggatgcaa accatatccc cgatgccgcc
     1861 atgaccctcg ccatcgtcgc gcttgctaca gggcaaacct gcacgcttcg caacatcggt
     1921 tcgtggcgcg tcaaagagac cgaccgcatt gccgcaatgg cgaacgagtt gcgcaaactc
     1981 ggtgcaaaag tcgccgaaga agccgaagca attcacatca ccccgcccga aacgccgaca
     2041 cccgacgccg tcatcgacac ttacgacgac caccgcatgg cgatgtgttt ctcgctggtt
     2101 tcgctgttgg gcgtacccgt cgtcatcaac gatccgaaat gcacccacaa aaccttcccg
     2161 acttatttcg acgtgttctc atcgctgacc gaaacagcgg aataaggcgg cattttgccg
     2221 cgattccggc gcggcgcggc gggcggctca ttctgtaaag aaagtatgtg cgccgaggta
     2281 gtttttggcg taaaacggtg tggagagttt ttcggttttg atggttttgc cgctgctggg
     2341 ggcatggatg aattcgccgt tgccgatata gagtccgacg tgtgagtagc ggtgtgcgcc
     2401 gccggtgttg aagaatacga ggtcgccggc cttaaggcgg ctgccgggga ttttgcggct
     2461 tgccgccgcc atgtcgcggg cggtgcgcgg cagcttgacg ttgagggcgt ttttgtaaac
     2521 gaattgaatc atgccgctgc aatcgaagcc ggttgcggtg ctgctgccgc cccatttgta
     2581 gggcgtgccg atgagtccga ggctgtggag catgagttcc tgcgagcctt gtgtgcggtc
     2641 gatgtggctg atgcggacgg cttggatttg ccggactgtc tgtttgggtt tcggttggcg
     2701 gtgtttgccg gaggtcgtgc cgcatgaggc gaggagcagt gcgctgagac agaggaaaag
     2761 ggttttgtcg gggggaaaca tggtttttcc tttgcgggtt cggatatccg tctgaaggtg
     2821 tttcagacg
//
LOCUS       DAXHRI010000195         2775 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXHRI010000195 DAXHRI010000000
VERSION     DAXHRI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.195"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            34..1929
                     /gene="mnmG"
                     /locus_tag="ACFM75_001962"
     CDS             34..1929
                     /gene="mnmG"
                     /locus_tag="ACFM75_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC6256440.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            1997..2581
                     /gene="rnhB"
                     /locus_tag="ACFM75_001963"
     CDS             1997..2581
                     /gene="rnhB"
                     /locus_tag="ACFM75_001963"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC6256441.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
BASE COUNT          738 a          885 c          693 g          459 t
ORIGIN      
        1 atgccgtctg aaacaccgtc aggcaataca cacatgaccc acatgattta ccccaaaacc
       61 tacgacgtta tcgtcgtcgg cggcggacac gccggcacag aagccgcgct cgccgccgcc
      121 cgtatgggcg cgcagacgct tttgctcaca cacaatattg aaacgctcgg acaaatgtcg
      181 tgcaaccctt ccatcggcgg catcggcaaa gggcatttgg tgcgcgaact cgacgcgctc
      241 ggcggcgcga tggcgttggc aaccgacaaa tccggcatcc agttccgccg tctgaacgcc
      301 agcaaaggcg cggcagtacg ggccacgcgc gcgcaggcgg atcgtatcct atataaagcc
      361 gccatccgcg aaatgttgga aaaccaagaa aacctcgacc ttttccaaca agccgtcgaa
      421 gacgtaacgc tcgatggcga ccgcatttca ggcgtaatca ccgcgatggg cgtggagttt
      481 aaagcacgcg ccgtggtgtt gaccgcaggc acgtttttgt ccggcaaaat ccacatcggt
      541 ttggaaaact acgagggcgg acgcgccggc gaccccgccg ccaaatcgct gggcggacgt
      601 ttgcgcgaat tgaacctgcc gcaaggccgt ctgaaaaccg gcacgccgcc gcgcatcgac
      661 ggacgcacga ttgacttctc ccaactgacc gaacagcccg gcgacacgcc cgttcccgtc
      721 atgtccgtac gcggcaacgc cgaaatgcac ccgcgccaag tgtcctgctg gattacgcat
      781 accaacacgc aaacccacga catcatccgc tcaggcttcg accgcagccc gatgtttacc
      841 ggcaaaatcg aaggcgtggg cccgcgttat tgtccgtcta tcgaagacaa aatcaaccgc
      901 ttcgccgaca aagacagcca ccagattttc ctagaacccg aaggcctgac tacgcacgaa
      961 tactacccca acggtatctc caccagcctg ccgttcgaca tccaaatcgc gctcgtccgc
     1021 agtatgaaag gtttggaaaa cgcccatatc ctgcgccccg gctacgccat cgaatacgac
     1081 tacttcgatc cgcgcaacct caaagcaagc ctcgaaacca aaaccattgc cggattgttt
     1141 ttcgccgggc aaatcaacgg tacgacaggt tatgaagaag ccgccgcgca aggactgctg
     1201 gcaggcgcga acgccgtgca atacgtccgc gaacaagacc cgctcctgct gcgccgcgaa
     1261 caagcctatc tcggcgtatt ggtggacgac ctcatcacca aaggcgtgaa cgaaccctac
     1321 cgaatgttca ccagccgtgc cgaataccgc ctgcaactca gggaagacaa cgccgatatg
     1381 cgcctgaccg aagacggcta caaaatcggc ttggtgggtg aagcgcaatg gcgcatgttc
     1441 aacgaaaaac gcgaagccat cgaacgcgaa atccaacgtt tgaaaacaac gtggtacacg
     1501 ccgcaaaaac tcgccgaaga cgaacaaatc cgcgtattcg ggcaaaagct cagccgcgaa
     1561 gccaacctgc acgacctcct gcgccgcccg aacctcgact acgccgcgct gatgacgctc
     1621 gaaggcgcga tgccgtctga acgcctctcc gccgaagtgg tcgaacaagt cgaaatccaa
     1681 gtcaaatacc aaggctatat cgaccgccaa aacgaagaaa tcgacagccg ccgcgacatc
     1741 gaaaccttaa agctgcccga ggatatcgat tacagcaaag ttaaaggttt gtcggcagaa
     1801 gtgcagcaaa agctcaacca gcacaaaccc gaaaccgtcg ggcaggcaag ccgcatttcc
     1861 ggcgtaaccc ctgcggcagt ggcattgctg atggtgcatt tgaagcgcgg gtttaaagac
     1921 gcgaaataaa cacatcggcg cgatgccgtc tgaaacaccg ttcagacggc atcgcgccat
     1981 cccgacagga aacatcatgc acatactgac cgccggcgtg gacgaggcag ggcgcgggcc
     2041 tttagtcggc agcgtgtttg ccgccgccgt catccttccg gaaatattcg acctgcccgg
     2101 actgaccgac tccaaaaaac tcagcgagaa aaaacgcgac gcgcttgccg aaatgataaa
     2161 aaatcaggcg gttgcgtggc acgttgccgc cgcctcgccc gaagaaatcg ccagcctcaa
     2221 catcctgcac gccaccatgc tcgcgatgaa acgcgccgtt gacggtttgg ctgtgcgtcc
     2281 cgaaagaata ttcatcgacg gcaaccgcat tcccgaacat ttgaacatcc ctgccgaagc
     2341 cgtcgtcaaa ggcgacagca aaatcatcga aatctccgcc gcatccgttt tggcaaagac
     2401 cgcacgcgat gcagaaatgt acgcactggc gcaacgccat ccccaatacg gtttcgacaa
     2461 acacaaaggt tacggcacga agcagcatct ggaagccctc aaacaatacg gcgtgctgcc
     2521 cgaacaccgc cgagacttcg cccccgtcag aaacctgctc gcgcagcagg ccttgtttta
     2581 aaccggcaca aaaatgccgt ctgaacttca gacggcattt tcagcatcgc aacttcaaag
     2641 agaaagaacc caaaccgtca ttcccgcgaa aaatagaaaa tcaaaaaaaa acctaaaatc
     2701 cgtcattccc gcgcaggcgg gaatccaatc cgtccggttt ccgttttttt tgaatttcag
     2761 gtaacttcca aaccg
//
LOCUS       DAXHRI010000196         2692 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXHRI010000196 DAXHRI010000000
VERSION     DAXHRI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.196"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(81..488)
                     /locus_tag="ACFM75_001964"
     CDS             complement(81..488)
                     /locus_tag="ACFM75_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC6256442.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECPVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            636..1745
                     /locus_tag="ACFM75_001965"
     CDS             636..1745
                     /locus_tag="ACFM75_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC6256443.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            1754..2581
                     /gene="fghA"
                     /locus_tag="ACFM75_001966"
     CDS             1754..2581
                     /gene="fghA"
                     /locus_tag="ACFM75_001966"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC6256444.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT          744 a          578 c          650 g          720 t
ORIGIN      
        1 tgcttacaat ccgcttttta aacaacaaat ttttgatttc tattacgaac aggacaaaaa
       61 tcctgcttat tgcactaaaa ctaagccgtt tcaggaattt gcggcaaatt tacggctttt
      121 accgaaccta atgctttcgc tttttggtaa aacgccaatt tgtattcaag caaatctaaa
      181 tagcgtttta attcggcaat ttgacacttc acattttcta tttgattttc aaacaaggaa
      241 aggcgttctt caatggtatc gtcgccaatg acgggacatt ccgcaaagcg tttgatgtct
      301 tttaagctca ttcccgtatt tttcaagcat tgcaataagc ccaaccattg caaatcgtta
      361 tcggtaaaac agcggttacc gtattcatca cgtccgatat taggcaacaa gccttcttta
      421 tcgtaaaaac gtagggtgtg ggcggagatg cctatttttt cggcggcttt ggcagtggta
      481 taagtcattt tccctccttc taaacaaaaa cagtaaaaaa cacttgcctt agagttaact
      541 ctaaagtgta aactgttgct atgttgctca ggtaaggcaa ctttgtcaat gaattaagag
      601 gaaagacaat ggaaatgaaa caaaccgatt caaccatcaa atctcgtgcg gcggtggcat
      661 tcgcccctaa ccaaccctta caaattgtgg aaatcgacgt agaaatgccg cgtaaaggcg
      721 aggtgttaat ccgcaatacc cacactggcg tgtgccatac tgatgcgttt acgttatcag
      781 gaagcgatcc tgaaggcgta ttccctgtgg tgcttggaca cgaaggtgcg ggtgtggtcg
      841 ttgctgtggg cgaaggtgtg tcaagcgtaa aaccgggtga tcacgtgatt ccgctttaca
      901 ccgccgaatg tggcgaatgt gagttttgtt gttcaggtaa aaccaacttg tgcgtctcag
      961 tgcgtgatac acaaggtaaa ggcttaatgc cggacggcac gacgcgtttt tcttatcaag
     1021 gtcagccaat ctatcactat atgggctgtt cgactttcag tgaatactcc gttgttgccg
     1081 aagtttcact ggcgaaaatc aaccctgaag ccaaccatga acaagtatgt ttgctcggct
     1141 gcggcgttac cacaggtatt ggtgcggtac ataatacggc aaaagtgcaa gaaggcgact
     1201 ctgttgccgt gtttggctta ggcgcgattg gtttggcggt ggtgcaaggt gcgcgtcaag
     1261 ccaaagccgg ccgcattatc gccattgata ccaatccatc aaaatttgag ttggcaaaac
     1321 agtttggtgc aacggattgt ttgaacccga acgattacga taaaccgatc aaagatgtgt
     1381 tgttagacat taataaatgg ggcattgacc atacctttga atgtatcggc aacgtaaacg
     1441 taatgcgtca ggcattagaa agtgcacatc gtggttgggg tcaatccatt atcatcggcg
     1501 tagcaggtgc aggacaagaa atttcaacgc gtccgttcca gttggtaaca ggtcgtgttt
     1561 ggaaaggttc agcatttggc ggtgtgaaag gtcgctctga actgccgaaa atggtggaag
     1621 attcaatgaa aggcgacatt gagttagaac cgtttgtaac ccacacaatg acactcgatc
     1681 aaatcaataa agcctttgac ttaatgcacg aaggtaaatc gatccgcgcc gttattcact
     1741 actaaggtat gcgatgaaac tgattgaaca acatcaaatt tttggcggtt cgcaacaagt
     1801 ttgggcgcat catgcccaaa cgctgcaatg cgaaatgaaa tttgccgtct atttgccaaa
     1861 taatccagaa aatcgaccgc ttggtgtgat ttattggctt tccggcttga cgtgtacgga
     1921 acaaaatttc attaccaaat caggctttca gcgttatgcg gcagaacatc aagtaattgt
     1981 ggtggcctcc gataccagcc ctcgcggaga gcaagtgccg aacgatgatg cttacgattt
     2041 aggacagggt gcaggctttt atttgaatgc gaccgaacag ccttgggcgg cgaattatca
     2101 aatgtatgat tacattttga acgagctgcc ccgtctgatt gagaaacact ttcctaccaa
     2161 cggcaaacgt tccattatgg ggcattcaat gggcggacac ggcgcattgg tattggcgct
     2221 gcggaatcag gaacgttatc aaagtgtttc tgccttttcg cctattttat cgccaagcct
     2281 cgtgccgtgg ggagaaaaag cctttactgc ttatttaggg aaagaccgtg aaaaatggca
     2341 gcaatatgat gcgaactcac tcattcaaca aggctataaa gtgcaaggta tgcgcatcga
     2401 tcaaggcttg gaagatgagt ttttgccgac acaattgcgt accgaagatt ttatcgaaac
     2461 ctgccgtgcg gcaaaccagc cggtcgatgt gcgtttccat aaaggctacg atcacagcta
     2521 ttacttcatc gccagtttta ttggcgagca tattgcttat cacgccgcgt ttttgaagta
     2581 aaccaaagag cgttcagtgt tcaaagcagt tttgggatag ccggcacgag ggcggtaaga
     2641 agtgccggca taaacgtatg ccgtctgagc cggaaggagc cgactctacg ga
//
LOCUS       DAXHRI010000197         2653 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXHRI010000197 DAXHRI010000000
VERSION     DAXHRI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.197"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>2653
                     /locus_tag="ACFM75_001967"
     CDS             <1..>2653
                     /locus_tag="ACFM75_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6256445.1"
                     /translation="VLKAGNDIDISTAHNRYTGNEYHESKKSGVMGTGGLGFTIGNRK
                     TTDDTDRTNIVHTGSIIGSLNGDTVTVAGNRYRQTGSTVSSPEGRNTVTAKSIDVESA
                     NNRYATDYVHTREQKGLTVALNVPVVQAAQNFVQAAQNVGKSKNKRVNAMAAANAAWQ
                     GYQAAQQMQQFAPSSSAGQGQNNNQSSGISVSVTYGEQKSRNEQKSRYTEAAASQIIG
                     KGQTTLAATGSGEQSNINITGSDVIGHAGTALIADNHIRLQSAKQDGSEQSKNKSSGW
                     NAGVAVKIGNGIRFGITAGGNIGKGKEQGGNTTHRHTHVGSTAGQTTIRSGGDTTLKG
                     AQLIGKGIQADTRNLHIESVQDTETYQSKQQNGNVQVTVGYGFSASGSYRQSKVKADH
                     ASVTEQSGIYTGEDGYQIKVRDNTDLKGGIITSTRSAESKGKNRFQTATLTHRDIQNH
                     SRYEGRSFGIGGSFDLNGGWDGTVTDKQGRPTDRISPAAGYGSDGDSKNSTTRSGINT
                     RNIHITDEAGQLARTGRTAKETEARIYTGIDTETADQHTGRLKNSFDKDAVAKEINLQ
                     REVTKEFGRNAAQAVAAVADKLGNTQSYERYQEARTLLEDELQNTDSEAEKAAIRASL
                     GQVNAYLAENQSRYDTWKEGGIGRSILHGAAGGLTTGSLGGILAGGGTSLAAPYLDKA
                     AENLGPAGKAAVNALGGAAIGYATGGSGGAVVGANVDWNNRQLHPSEYKLAEQYADIV
                     ASRLNISKEEALGRIIRHMQRTVDYATAKADNFRTDDAIISILGANNLPKQDQHYTNP
                     NYNKQYIQNYVRDFNLANKYNYFGKTPTQSRDYKVGVVTDIGKAALNSGIGIFESAAN
                     LATGEIDPNSWAYVHMGRIGYTHPEYGN"
BASE COUNT          849 a          696 c          661 g          447 t
ORIGIN      
        1 tgtcttaaaa gcaggaaacg acatcgatat ttctactgcc cataatcgct acaccggcaa
       61 tgaataccac gagagcaaaa aatcaggcgt catgggcaca ggcggattgg gctttactat
      121 cggtaaccga aaaactaccg atgacactga tcgtaccaat attgtccata caggcagcat
      181 tataggcagc ctgaatggag acaccgttac agttgccgga aaccgctacc gacaaaccgg
      241 cagtaccgtc tccagccccg aggggcgcaa tactgtcact gccaaaagca tagatgtaga
      301 gtccgcaaac aaccggtatg ccactgacta cgtccatacc cgggaacaaa aaggcctcac
      361 cgtcgccctc aatgtcccgg ttgtccaagc tgcacaaaac ttcgtacaag cagcccaaaa
      421 tgtgggcaaa agtaaaaata aacgcgttaa tgccatggct gcagccaatg ctgcatggca
      481 gggttatcaa gcagcccaac aaatgcaaca atttgctcca agcagcagtg cgggacaagg
      541 tcaaaacaac aatcaaagct ccggtatcag tgtgtccgtt acctacggcg aacagaaaag
      601 ccgtaacgag caaaaaagcc gttacaccga agcggcagca agtcaaatta tcggcaaagg
      661 gcaaaccaca cttgcggcaa caggaagtgg ggagcagtcc aatatcaata ttacaggttc
      721 cgatgtcatc ggccatgcag gtactgccct cattgccgac aaccatatca gactccaatc
      781 tgccaaacag gacggcagcg agcaaagcaa aaacaaaagc agtggttgga atgcaggcgt
      841 agccgtcaaa ataggcaacg gcatcaggtt tggaattacc gccggaggaa atatcggtaa
      901 aggtaaagag caagggggaa atactaccca ccgccacacc catgtcggca gcacagccgg
      961 acaaaccacc atccgaagcg gcggggatac caccctcaaa ggtgcgcagc tcatcggcaa
     1021 aggcatacag gcagatacgc gcaacctgca tatagaaagt gttcaagata ctgaaaccta
     1081 tcagagcaaa cagcaaaacg gcaatgtcca agttactgtc ggttacggat tcagtgcaag
     1141 cggcagttac cgccaaagca aagtcaaagc agaccatgcc tccgtaaccg agcaaagcgg
     1201 tatttatacc ggagaagacg gctatcaaat caaagtcaga gacaacacag acctcaaggg
     1261 cggtatcatc acctccaccc gaagtgccga aagcaaaggg aaaaaccgct ttcagacggc
     1321 caccctcacc catcgcgaca ttcaaaacca cagccgctac gaaggcagaa gcttcggcat
     1381 aggcggcagt ttcgacctga acggcggctg ggacggcacg gttaccgaca aacaaggcag
     1441 gcctaccgac aggataagcc cggcagccgg ctacggcagc gacggagaca gcaaaaacag
     1501 caccacccgc agcggcatca acacccgcaa catacacatc accgacgaag cgggacaact
     1561 tgcccgaaca ggcaggactg caaaagaaac cgaagcgcgt atctacaccg gcatcgacac
     1621 agaaactgcg gatcaacaca caggccgtct gaaaaacagc ttcgacaaag acgcggtcgc
     1681 caaagagatc aacctgcaaa gggaagtaac gaaggagttc ggcagaaacg ccgcccaagc
     1741 cgtagcggcc gttgccgaca aactcggcaa tacccaaagt tacgaacggt atcaggaagc
     1801 ccgaaccctg ctggaggacg aactgcaaaa cacggacagc gaagccgaaa aagccgccat
     1861 ccgcgcatcc ctcggccaag taaacgccta tcttgccgaa aaccaaagcc gctacgacac
     1921 ctggaaagaa ggcggcatag gcaggagtat cctgcacggg gcggcaggcg gactgacgac
     1981 cggcagcctc ggcggcatac tggccggcgg cggcacttcc cttgctgcac cgtatttgga
     2041 caaagcggcg gaaaacctcg gcccggcagg caaagcggcg gtcaacgcac tgggcggtgc
     2101 ggccatcggc tatgcaactg gtggtagtgg tggtgctgtg gtgggtgcga atgtagattg
     2161 gaacaatagg cagctgcatc cgagtgaata taaattagca gaacaatatg cagatatagt
     2221 ggcttctcgt ctaaacattt caaaagaaga agcacttggg cgaattattc ggcatatgca
     2281 acgaaccgta gattacgcaa cggctaaagc ggataatttc cgtactgatg acgccattat
     2341 ttcaatttta ggagcaaaca atttacccaa gcaagatcaa cattacacta atccgaacta
     2401 caacaaacaa tacattcaaa attatgttcg tgatttcaat ttagccaata aatacaatta
     2461 ttttgggaaa actcctactc aatcaaggga ttataaagtt ggagttgtta cagatattgg
     2521 taaggctgca ttaaatagcg gtataggtat atttgaatcg gctgcgaatt tagcgacagg
     2581 ggagatagat ccgaactcat gggcttatgt acacatgggg cgtataggtt atacacatcc
     2641 tgaatatgga aat
//
LOCUS       DAXHRI010000198         2612 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXHRI010000198 DAXHRI010000000
VERSION     DAXHRI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.198"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            195..785
                     /locus_tag="ACFM75_001968"
                     /pseudo
     CDS             195..785
                     /locus_tag="ACFM75_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            890..1558
                     /gene="hda"
                     /locus_tag="ACFM75_001969"
     CDS             890..1558
                     /gene="hda"
                     /locus_tag="ACFM75_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC6256446.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            1555..2223
                     /locus_tag="ACFM75_001970"
     CDS             1555..2223
                     /locus_tag="ACFM75_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6256447.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEEEAKEKGWPV
                     LNFK"
BASE COUNT          709 a          798 c          576 g          529 t
ORIGIN      
        1 cctcttaaaa cagttttaaa aattccctaa tctcgtcagc tttttcataa aaccgcgcat
       61 tccggcgcgg ttttatgaaa aagctggcgt aacttttccg gatacaaaaa aacgccgaaa
      121 tccacgtctt ccgaagattt cagcgttttt ttactctatt tgttccttgt gcaaaaacta
      181 acagtccccc acaggaaaac gtcgctctgt tccgccacaa cggcgaaatc ctgttgagcg
      241 gcatcaacat caacctcaaa aacggcgatt ccctgctgat acgcggcccg agcggttgcg
      301 gcaaaacctc gctgttgcgc gcgccggcgg ggctttggcc gttcggcagc agcggcaaag
      361 tcagccgtcc gccgcatcaa gacatcctct tcctgccgca acgcccgtac acggcacagg
      421 gcagcctgcg cgacgcggtt tgttaccccg atatcgacaa acagcatccc gagttagccg
      481 aagccatgaa cacctgccgc ttgggttatc tgattgacaa attggacaaa acagacgact
      541 ggcagcacaa actctcccct ggcgaactgc aacgcgtcgc cttcgtccgc gccctgctct
      601 cgcagcccaa agttatcctg ctcgacgaag ccaccgccgc cttggacgaa ccggccgaag
      661 ccgcgctcta ccgcgcctta aagcaaaaac tgcccgacag catcatcatc agcatcggac
      721 accgaagcac gctcaacgcg ttccacaata tgcacctcga tgtcggcaac gttgcctgcg
      781 gctgaacgat gccgtctgaa gccgttttca gacggcattt ttcaaattga taaagcacgc
      841 gaaaacaacg tacaataaac ccgtttacca cccctcggaa tgccgaaccg tgaaccagct
      901 tattttcgac tttgccgcac acgactatcc gagtttcgac aaattcctcg gcacggaaaa
      961 cgcggaactg gtctatgtcc tccgacacaa acacggacag tttatctatg tctggggtga
     1021 ggaaggtgcg ggcaaaagcc atcttttgca ggcgtgggtc gcacaggcgc tcgaagccgg
     1081 caaaaacgcc gcctatatcg atgccgcctc catgccgctg accgatgccg cgttcgaggc
     1141 ggaatacctc gccgtcgatc aagtcgaaaa gctgggcaac gaagaacaag ccctgctttt
     1201 ttccatcttc aaccgcttcc gcaacagcgg caaaggcttt ttgctgctcg gttcggaata
     1261 cacgccccag cagctcgtca tccgcgaaga tttgcggacg cgtatggcgt actgcctcgt
     1321 ttacgaagtc aagcccttaa ccgaccaaga aaaaatcgac gcgctcgcca gtatggcggc
     1381 ggcacgccaa gtaaccgtcg attccgaaat tttcgaatac ctgctcaaac actggcggcg
     1441 cgatatggac agcctgatga tgatgctcga cacgctggac aactacgccg tcacaatggg
     1501 caaacgcatc accctgccgc ttttgcgcca gcttttgaaa caacaggaaa cccaatgaaa
     1561 aacctcgcca tcttcgacct cgacaacacc ctcatcaaca ccgattccga ccacgcctgg
     1621 ccgcaatacc tcatcaaaaa aggcttggtt gacgctgccg aaaccgaagc gcaaaacgaa
     1681 aaattctacc gcgactacca aaacggctgc ctcgacatcg acgcattcct caaattccac
     1741 ctcgccccgc tcgcccgcta cagcaaagaa gagctggcag agtttcaccg cgaatttatg
     1801 gcggaataca tcatccccca catctcgcct atgcagcgta tgctggtgca gagccaccaa
     1861 atggcaggcg acgaaaccct tgtgatttcc tcaaccaacg agttcatcat cacccccgtc
     1921 tgccgccttt tcggcatcac caacatcatc ggcacacaac tcgaaaccgg ttctgacggc
     1981 cgctacaccg gcaattacat cggcacgccc agcctcaaag aaggcaaaat cacccgcctg
     2041 aaccaatggc ttgccgaacg cggcgaaacg ctcgaaagct acggcaaaac ctatttttac
     2101 agcgactcca aaaacgacct gccgctgctg cgccttgtca gcgaacccgt cgccgtcaac
     2161 cccgatgccg agctggaaga agaagccaaa gaaaaaggct ggccggtttt aaatttcaaa
     2221 tgacgcaatg ccgtctgaag cccgattcgg cgttcagacg gcattgaggc tgaaatccaa
     2281 aaaactcaaa gccacaggaa tttatccgga aaaacaaaaa acacaggaat tcatcgggaa
     2341 aaacaacaat ctttccaccg tcattcccac gaaagtggga atcttgatcc gtccgcacgg
     2401 aaacttatat ttcgtcattt gcacccgaca taattcaata ctttattttg caataaatta
     2461 acggatgctg acaaattaga ttggattaat taccctatta tccatattgg gttatcgggt
     2521 tgctaattga gcagagcgta gcaaaccata aagtgagtgt ttccacccac tctgcattta
     2581 tagtggatta acaaaaatca ggacaaggcg ac
//
LOCUS       DAXHRI010000199         2598 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXHRI010000199 DAXHRI010000000
VERSION     DAXHRI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.199"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..169)
                     /locus_tag="ACFM75_001971"
     rRNA            complement(<1..169)
                     /locus_tag="ACFM75_001971"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(576..651)
                     /locus_tag="ACFM75_001972"
     tRNA            complement(576..651)
                     /locus_tag="ACFM75_001972"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(616..618),aa:Ala,seq:tgc)
     gene            complement(657..733)
                     /locus_tag="ACFM75_001973"
     tRNA            complement(657..733)
                     /locus_tag="ACFM75_001973"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(697..699),aa:Ile,seq:gat)
     gene            complement(834..2374)
                     /locus_tag="ACFM75_001974"
     rRNA            complement(834..2374)
                     /locus_tag="ACFM75_001974"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          563 a          743 c          547 g          745 t
ORIGIN      
        1 gtctatgtat tcaacttagg atactgcaca gaatgcagtg ggtttcccca ttcggacatc
       61 gcgggatcat tgctttattg ccggctcccc cgcgcttttc gcaggcttac acgtccttcg
      121 tcgcctatca tcgccaaggc atccacctga tgcacttatt cacttgactc tatcatttca
      181 agaacttctt tgacttcgtt tgcctacctg ttgactaagt aaaagcaaac ttgaaattcc
      241 tactttgata aagcttactg ctttgttgtg tcttaatcct gccttttgtg tttcaggatt
      301 aagtcgatac aatcatcacc caaatactgt gtttgttttc ttttctctcg cgagagattt
      361 ttaccctttg caaaaggtaa aaatcaaaac aaactcattg tctttgtttg ttgatttcgg
      421 ctttccaatt tgttaaagat cgatgcgttc gatattgcta tctactgcgc aaatcaaaac
      481 gagctgatta ttatatcagc attttgttct tggtcaagtg tgacgtcgcc ctgaatggat
      541 tctgttccat tcttccgttt tgatttgtac agtattggtg gaggcaaacg ggatcgaacc
      601 gatgaccccc tgcttgcaaa gcaggtgctc taccaactga gctatgcccc cgttcttggt
      661 gggtctggga ggacttgaac ctccgacccc acgcttatca agcgtgtgct ctaaccagct
      721 gagctacaaa cccggattct cttcttaagc gaatcttgcc ttcactcaag cttcttccgc
      781 atcttttcag tttaccgata agtgtgaatg cctaaagcct cttctttctc tagaaaggag
      841 gtgatccagc cgcaggttcc cctacggcta ccttgttacg acttcacccc agtcatgaag
      901 cataccgtgg taagcggact ccttgcggtt accctaccta cttctggtat cccccactcc
      961 catggtgtga cgggcggtgt gtacaagacc cgggaacgta ttcaccgcag tatgctgacc
     1021 tgcgattact agcgattccg acttcatgca ctcgagttgc agagtgcaat ccggactacg
     1081 atcggttttg tgagattggc tccgcctcgc ggcttggcta ccctctgtac cgaccattgt
     1141 atgacgtgtg aagccctggt cataagggcc atgaggactt gacgtcatcc ccaccttcct
     1201 ccggcttgtc accggcagtc tcattagagt gcccaactga atgatggcaa ctaatgacaa
     1261 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacagccat
     1321 gcagcacctg tgttacggct cccgaaggca ctcctccgtc tccggaggat tccgtacatg
     1381 tcaagaccag gtaaggttct tcgcgttgca tcgaattaat ccacatcatc caccgcttgt
     1441 gcgggtcccc gtcaattcct ttgagtttta atcttgcgac cgtactcccc aggcggtcaa
     1501 tttcacgcgt tagctacgct actaagcaat caggttgccc aacagctaat tgacatcgtt
     1561 tagggcgtgg actaccaggg tatctaatcc tgtttgctac ccacgctttc gggcatgaac
     1621 gtcagtgtta tcccaggagg ctgccttcgc catcggtatt cctccacatc tctacgcatt
     1681 tcactgctac acgtggaatt ctacctccct ctgacacact cgagtcaccc agttcagaac
     1741 gcagttcccg ggttgagccc ggggatttca catcctgctt aagtaaccgt ctgcgcccgc
     1801 tttacgccca gtaattccga ttaacgctcg caccctacgt attaccgcgg ctgctggcac
     1861 gtagttagcc ggtgcttatt cttcaggtac cgtcatcagc cgctggtatt agcaacagcc
     1921 ttttcttccc tgacaaaagt cctttacaac ccgaaggcct tcttcagaca cgcggcatgg
     1981 ctggatcagg cttgcgccca ttgtccaaaa ttccccactg ctgcctcccg taggagtctg
     2041 ggccgtgtct cagtcccagt gtggcggatc atcctctcag acccgctact gatcgtcgcc
     2101 ttggtgagcc tttaccccac caactagcta atcagatatc ggccgctcga atagcgcaag
     2161 acccgaaggt cccctgcttt ctctctcaag acgtatgcgg tattagctga tctttcgatc
     2221 agttatcccc cactactcgg tacgttccga tatgttactc acccgttcgc cactcgccac
     2281 ccgagaagca agcttctctg tgctgccgtc cgacttgcat gtgtaaagca tgccgccagc
     2341 gttcaatctg agccaggatc aaactcttat gttcaatctc taacttttta acttctggtc
     2401 tgcttcaaag aaaccgacag gacaatgttc aaaacattat cttgtctgtc tttcaaacag
     2461 tgtgaggctc aaggcactca cacttatcgg taatctgttt tgttaaagag cgtttgaatt
     2521 ataaagtatt tcttccgcct gtcaagatat ctctcgatat ccccaacatt ctgtgctata
     2581 cttttcagtt cgcccgcc
//
LOCUS       DAXHRI010000200         2581 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXHRI010000200 DAXHRI010000000
VERSION     DAXHRI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.200"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            74..703
                     /locus_tag="ACFM75_001975"
     CDS             74..703
                     /locus_tag="ACFM75_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC6256448.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            849..2525
                     /locus_tag="ACFM75_001976"
     CDS             849..2525
                     /locus_tag="ACFM75_001976"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC6256449.1"
                     /translation="MLTYTPSDARPPAKTHEKPWLLMLMAFAWLWPGVFSRDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVVHFLNPAAAAFAAAGLV
                     LHGYSLVRRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
BASE COUNT          505 a          659 c          671 g          746 t
ORIGIN      
        1 tggggttgaa gttgacatct acaaagttta atgtttgaat acattaaaaa aatatatttg
       61 aatgagatgt tttatgattc aaaatgttgt tacttcaata atcctgtatt ctgggacagc
      121 cgtagactta cttattatcc taatgttatt ttttgccaaa agaaaaagca gaaaagacat
      181 cattaacatc tatttaggac aatttctagg ctctgttagc ctgatattgc taagtttgct
      241 ttttgcattt gtcttagatt atattcctag taaagagatt ttaggtttgc tcggcttgat
      301 tccaattttc ctaggcatca aagttttgct gttaggagat tctgatggag aggctattgc
      361 caaagagggt ttgcgcaaag ataataaaaa cctgattttt ctagtcgcta tgattacttt
      421 tgcaagttgt ggtgctgaca atattggtgt ctttgtccca tattttacta ccttaaattt
      481 agcgaatttg atagtggctt tacttacctt tctagtcatg atttatctct tggttttttc
      541 tgcccaaaaa ttagcacaag tcccttctgt tggagaaact ttggaaaaat atagcagatg
      601 gtttgttgct gttgtttatt taggattggg ggtatatatc ctgattgaaa acaacagttt
      661 tgatatgcta tggactgtgt cgggccagga aaaaatatta tgaaaaagat agtatctgca
      721 aagactgcca tgtgcagtct tttttgttgc cggtcttttt gtgtctgatg ccgtctgaag
      781 cagtctctgc acgacctttg tgcgaatatt tgctacactt ggcaatccgt ccatttcttc
      841 tcacgcttat gctgacctac actccgtccg atgcccgccc gcccgccaaa acccacgaaa
      901 aaccgtggct gctgatgctg atggcgtttg cctggttgtg gcccggcgtg ttttcccgtg
      961 atttgtggaa tcctgccgaa cctgccgtct ataccgccgt cgaagcactg gcaggcagcc
     1021 ccaccccctt ggttgcccat ctgttcgggc atgccgattt cggcatacca cccgtgtatc
     1081 tttgggttgc cgccgcgttc aaacatttgc tgtcgccgtg ggcagccgac ccgtatgatg
     1141 ccgcacgctt tgcaggcgtg tttttcgccg ttgtcggact gacttcctgc ggctttgctg
     1201 gcttcaactt tttgggcaga caccacgggc gcagcgtcgt cctgattctc atcggctgta
     1261 tcgggctgat tccggttgtc cattttctca atcccgccgc cgccgccttt gccgccgccg
     1321 gactggtgct gcacggctac tcgctggtac gccgccgtgt gattgccgcc tctttcctgc
     1381 tcggtacggg ctggacgctg atgtcgctgg cggcagctta tccggcagca tttgccctga
     1441 tgctgccctt gcccgtactg atgtttttcc gtccgtggca aagcaggcgt ttgatgttga
     1501 cggcagtcgc ctcacttgcc tttgccctgc cgcttatgac cgtttacccg ctgctcttgg
     1561 caaaaacgca gcccgcgctg ttcgcgcaat ggctcgacga tcacgttttc ggtacgttcg
     1621 gcggcgtgcg gcacgttcag acggcattca gtttgtttta ctatctgaaa aacctgcttt
     1681 ggtttgcatt gcctgcgctg ccgctggcgg tttggacggt ttgccgcacg cgcctgtttt
     1741 cgaccgactg ggggattttg ggcattgtct ggatgcttgc cgttttggtg ctgcttgccg
     1801 tcaatccgca gcgttttcag gataacctcg tctggctgct tccgccgctt gccctgttcg
     1861 gcgcggcgca actggacagc ctgaggcgcg gcgcggcggc gtttgtcaac tggttcggca
     1921 ttatggcgtt cggactgttt gccgtgttcc tgtggacggg ctttttcgcc atgaattacg
     1981 gctggcccgc caagcttgcc gaacgcgccg cctacttcag cccgtattac gttcccgaca
     2041 tcgatcccat tccgatggcg gttgccgtac tgttcacacc cttgtggctg tgggcgatta
     2101 cccgcaaaaa catacgcggc aggcaggcgg ttaccaactg ggcggcaggc gttaccctga
     2161 cctgggcttt gctgatgacg ctgttcctgc cgtggctgga cgcggcgaaa agccacgcgc
     2221 ccgtcgtccg gagtatggag gcatcgcttt ccccggaatt aaaacgggag ctttcagacg
     2281 gcatcgagtg tatcgacata ggcggcggcg acctgcacac gcggattgtt tggacgcagt
     2341 acggcacatt gccgcaccgc gtcggcgatg tacaatgccg ctaccgcatc gtccgcttgc
     2401 cccaaaatgc ggatgcgcct caaggctggc agacggtctg gcagggtgcg cgcccgcgca
     2461 acaaagacag taagttcgca ctgatacgga aaatcgggga aaatatatta aaaacaacag
     2521 attgagccga atttctggat taagtgccgg aaatcgcgta taattgcgcg attaaacctt
     2581 t
//
LOCUS       DAXHRI010000201         2580 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXHRI010000201 DAXHRI010000000
VERSION     DAXHRI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.201"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(197..268)
                     /locus_tag="ACFM75_001977"
     tRNA            complement(197..268)
                     /locus_tag="ACFM75_001977"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Arg,seq:cct)
     gene            complement(288..734)
                     /locus_tag="ACFM75_001978"
     CDS             complement(288..734)
                     /locus_tag="ACFM75_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC6256450.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKARVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(738..1712)
                     /gene="ispB"
                     /locus_tag="ACFM75_001979"
     CDS             complement(738..1712)
                     /gene="ispB"
                     /locus_tag="ACFM75_001979"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC6256451.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1936..2244
                     /gene="rplU"
                     /locus_tag="ACFM75_001980"
     CDS             1936..2244
                     /gene="rplU"
                     /locus_tag="ACFM75_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC6256452.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2269..2541
                     /gene="rpmA"
                     /locus_tag="ACFM75_001981"
     CDS             2269..2541
                     /gene="rpmA"
                     /locus_tag="ACFM75_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC6256453.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          758 a          695 c          573 g          554 t
ORIGIN      
        1 cataaaacaa cagatttcca agccggaaca tcttttacga agcctgaaaa tcatttcatg
       61 attctaccgt cctaaaggtc ggtttttcaa gcaggaagaa aaagttttca gatggcaaaa
      121 aagccctcca acactgaaag gccttatatc ggaaacttcc cgcaacacgg gaaacagaca
      181 aatgcaaatc gtcaaacctc gccaacagga atcgaacctg tattttacgc ttaggaggca
      241 tacgttctat ccgttgaact atggcgagcc gaaatgaaaa ggagatttta accctttccg
      301 acgacaaaag acaagatgcc ggccgcaatc agcggcccga cagggatacc gcccataaat
      361 gccaccccga taaccgtccc gattaacagc cctgtaatta aaacaggctg ctgtcccatc
      421 aaaggcacgc cgcgtcccgc cagccaagcc acgaaaatac cgataaaaac ggcggatatc
      481 attttaaaat tcaaaaattc ggcaacagga ggaacccgcg cctttcctga aaccaacgga
      541 ctcaaaaccc ctatggtcaa aagaatgata ccgaggttca acccgtgctt ctcgaccaac
      601 gggacaaact gtaccaatgc cgtctgctgc atcagcagca atatggttgc cgagatggta
      661 atcgaattgt tgttgctgac cacccccaac agaatcagcg taaccaaaaa caggggaaca
      721 aaactgaaat tcatcggtta cgacaccctg accaaagatt ccttcgccag ctgaatcatg
      781 gcatccttca cttcgctgtc gggcagggca tccaaggcgg caacggcaca atcgactgct
      841 ttgcgcgcct cgcctattga atatgccaaa gcatccgaac ggacgacata atcgtggatt
      901 ttctcaaaat agctgcgatc tgcattttcc aaagcagtac gcacatcgtt cgcaacctgt
      961 tcggaaccct gacgcatcag ataaatcaaa ggcaaagtcg gttttccttc cgccaaatcg
     1021 tcgccgacgt ttttgccggt ttcttcggtt tcgccagaat agtccagcac atcgtcaata
     1081 atttggaatg ccgtaccgac atacataccg tagtctttca acgcccgttc gtgttcgggg
     1141 gaagccttgc ccaaaattgc gccgacttga gcggcagctt caaacaattt tgccgtttta
     1201 tattggatga cttggatata ttgttcttcg gtaatgtccg tattgccgat gttcatcagc
     1261 tgcatgactt cgccctcggc aataatgttg gttgcatccg ccatcacttc caaaacgcgc
     1321 atactgcccg aggcaaccat cagttgaaag gcgcgcgtgt ataaaaagtc gccaaccaac
     1381 acagccgccg cattgccgaa cagattgttt gccgttgccc gcccacggcg caaatcgctt
     1441 tcatcgacga catcgtcgtg caggagcgtc gaagtgtgga taaactcgac catcgccgcc
     1501 agcgaataca gtttctcgtc atcataaccg accgccttac ccgccaaaat cgtcataatc
     1561 ggacgcaggc gtttgccgcc cgcgctgatg atatatgtac cgatttgcga aatcagtgcg
     1621 acatcggatt gcaccgcacg gttgatgact tcattgactt tggcaaggtc ttcaggcaga
     1681 tgtcgctgga aatagggcag attctcgagc atggcagact ctcaggttta cgtttccgtc
     1741 aaacggcaga taccgcttga aagctgattc aaatagcggg caattatatc agcaaagcta
     1801 tgaggacaca tcttttcaga cggcatatgg ttatccgaac cgcgcaaact ttgacaaaac
     1861 aggcaaataa caatacaatg ccgagttccg cacaatggtg tgcggatatt aaccataacc
     1921 cttatggagt tgagtatgta cgcggtcgta aaaaccggcg gcaaacagta taaagtttcc
     1981 gtcggcgaaa aattgaaagt agaacagata ccagccgaac tcgacagcca aatcgaactg
     2041 accgaagttt tgatgattgc tgacggcgaa tctgtaaaag ttggcgcacc ctttatcgaa
     2101 ggtgcaaaag taacggctaa agtagtggca cacggtcgtg gcgaaaaagt ccgcatcttc
     2161 aaaatgcgcc gccgcaaaca ctaccaaaaa cgccaaggcc accgccaaaa tttcacccaa
     2221 atcgaaatcg tggcaatcgc ctaaccttaa aacttaggag cattacaaat ggcaagtaaa
     2281 aaagcaggcg gcagcacccg caacggtcgc gattcagaag ccaaacgctt gggcgttaaa
     2341 gcctacggca acgagctgat tcccgcaggt tccatcatcg tacgccaacg cggtaccaaa
     2401 ttccacgcag gcgacaacgt aggtatgggc aaagaccaca ctttgttcgc caaagttgac
     2461 ggttatgtcg aattcaaaac caaaggcgcg ctgaaccgta aaactgtcag catccgtcct
     2521 tacaccggtt ctgaagaata atccaaccga tacctgaagc cgcatctttt cacgatgcgg
//
LOCUS       DAXHRI010000202         2572 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXHRI010000202 DAXHRI010000000
VERSION     DAXHRI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.202"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..275)
                     /locus_tag="ACFM75_001982"
     CDS             complement(<1..275)
                     /locus_tag="ACFM75_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HFC6256454.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PFGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKT"
     gene            431..583
                     /locus_tag="ACFM75_001983"
                     /pseudo
     CDS             431..583
                     /locus_tag="ACFM75_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            735..827
                     /locus_tag="ACFM75_001984"
                     /pseudo
     CDS             735..827
                     /locus_tag="ACFM75_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1033..1266
                     /locus_tag="ACFM75_001985"
                     /pseudo
     CDS             1033..1266
                     /locus_tag="ACFM75_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1194..1778)
                     /locus_tag="ACFM75_001986"
     CDS             complement(1194..1778)
                     /locus_tag="ACFM75_001986"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFC6256455.1"
                     /translation="MASIILNYLEKDGRPVIHGMGYISEWKINNGKVFYNISDTILNE
                     LLLKSGRSMFLLMDWSVFNSLPGKYCGILYKLCKDYAGIGKTPYFTVQEFRHYLGLND
                     KEYEEFRALKAKIIKPAIEHINNSENVDIKIIPQLNQNMADEDIETAFSESKIEPLAT
                     LIKFLETKTPRSSSGLERLCHCPSRTALNMDNSG"
     gene            complement(2213..>2572)
                     /locus_tag="ACFM75_001987"
     CDS             complement(2213..>2572)
                     /locus_tag="ACFM75_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256456.1"
                     /translation="NPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVFWDGNK
                     RTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNCISGID
                     YFGTDEDIDFPDSPNID"
BASE COUNT          721 a          555 c          489 g          807 t
ORIGIN      
        1 gttttaggag cagctttatc agcaataatg ccatcagcaa aagcaatatt ggccgtacct
       61 acagccaaac ataaagaaaa acccaataaa gaaaaagaaa agatatttga acgacaaaca
      121 ggtgcatgag tagtaccaaa aggaacagat ttcacatgag cgctgcctga atcactatcg
      181 gcacagcttt taccttcgcg cttggtagtt tcagcaacgg ctaccacagc cccacgtttg
      241 cggttgaaaa ttacacgata gagagtttta ttcatgattt cagttatttg atttttatag
      301 agttattaga aaaaattgga tagtctgacc attctagatc aaggattttg gcgagtcaat
      361 taccgccatt ttactgccat ttgtttatta aattagggac tttactagat aacggttaaa
      421 aatcccattc gaacgaaatg gcaaggttta taccgtcgtt gtccctaatg cgcaatcagc
      481 aacattattg ccgattatcc gagagaaagt taagtctgat ggcattgtgt atacggatac
      541 ctttcgtagt tatgatgtac ttgatgtcag tgaatttagc catttacgca agttttccag
      601 tatttgactg gcaatttaaa acagtcggat tttgtcccat ttgttggcca agtctttact
      661 tgcttggccg tttgaattta aaaagcagtc tttctacttt ccgacctttt tttctgttgt
      721 aaggtctatg atccaatagc attcccaaag agcattttgg acggtattta aaggaatgtg
      781 aatagcgttt taacaacagt gaaatagaat ctaaaattca gatttgaaga caattagtta
      841 atgggaattt ggtctggtta tctagtacag cctctaaaat tttagtgacc gaacaaactc
      901 gaaaaatcca catcgccgat gcgttccagc cggtcggcaa agctgtcaag attcacagat
      961 tcagcgtttg agacctttgc aaaattcccc aaaatcccct aaattcccac caagacattt
     1021 aggagatttc tcatgagcac cttcttccgg caaaccgccc aagccatgat tgccaaacac
     1081 atcgaccgtt tcccactatt gaagttggac caggtgattg attggcaacc gatcgaacaa
     1141 tacctgaacc gtcaaaaaac ccgttacctc cgagaccacc gcggtcgtcc cgcctacccg
     1201 ctgttgtcca tgttcaaagc cgttctgctc ggacaatggc acagcctctc caatcccgaa
     1261 ctcgaacgtg gtgttttggt ttccagaaat ttaatcagtg tggctagcgg ctctattttc
     1321 gattcagaaa aggctgtttc tatatcctcg tctgccatgt tctggtttaa ttgcggtata
     1381 attttgatgt ccacattttc actgttgttg atatgctcta ttgccggttt aattattttg
     1441 gccttcaacg ctctaaattc ttcgtattct ttgtcattca atcccaaata gtgtctaaat
     1501 tcttgaacgg taaaataggg ggttttgccg atgcctgcgt aatccttgca caatttgtat
     1561 aggattccgc aatattttcc cggtaagcta ttgaacacac tccaatccat cagcaaaaac
     1621 atgcttcttc cgcttttcaa tagtaactca ttaagaatgg tgtctgatat attgtagaaa
     1681 actttgccat tgttgatctt ccactctgaa atatagccca taccgtgtat gacgggacgg
     1741 ccgtctttct ccaaataatt caaaatgatt gatgccattc gtagagcatc caagtctttt
     1801 ttagttgttt tctttgaagt tgatatccaa tacaacgttc cagatctaag atatctatcg
     1861 caacaaaacc gttgtcatca taagtaccac cttattcaaa atgtgtttta atcttctgca
     1921 aaaaaagata ccaatacttg tattgcattt cagtcaagga atggcccatg tggattaagg
     1981 attgcggtgt tttttgttta tcaatatggt ttgatgtcat tttgatttcc atttcatgtg
     2041 gctactatat ctctctatta tgcactaata cacagagttt cttgatgtta gaacgaatac
     2101 ggcgtccgtg tcggcgtgaa attctgattt tccttatacg aaaccgcaaa cctttattac
     2161 atctcctgaa taatttagga tactttttag gtattgaaaa acaccttggt ttttagtcta
     2221 tgttcggtga gtcggggaaa tctatatctt cgtccgtgcc gaaatagtct atgcccgata
     2281 tacaattttg atacacaaac ttggaaatat cggtatcgtc gccggagcga tagaatgcgg
     2341 acagtttttc attgaattgc ggcatctgca tttcggagat ttccaagatg ccgcagcccc
     2401 ccgccatcat cagaccgttg gcaaataagg ttgccgttcg tttgttgccg tcccaaaaaa
     2461 cctgctgccg catacaataa agcataaaac ggacggctgc ctccgtcgtc gaaccgcttt
     2521 gcagtccgat attttgtaac acgcgggcca cttcaatttc cttcactgga tt
//
LOCUS       DAXHRI010000203         2545 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXHRI010000203 DAXHRI010000000
VERSION     DAXHRI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.203"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..859
                     /gene="hrpB"
                     /locus_tag="ACFM75_001988"
     CDS             <1..859
                     /gene="hrpB"
                     /locus_tag="ACFM75_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HFC6256457.1"
                     /translation="VHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQYQSSLA
                     AERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYLNRWQL
                     DGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQFFYATA
                     IQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNHQFYLG
                     ADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQTTNTV
                     YGFNLNYSF"
     gene            968..>2545
                     /locus_tag="ACFM75_001989"
     CDS             968..>2545
                     /locus_tag="ACFM75_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFC6256458.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFLVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGTPQIGKDGALTGFDVRQGTLTVGT
                     SGWNDKGGADYTEVLARAVALQGKLQGKNLAVSIGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGSANLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIEAGKPLSLETSNVASNIRLNNGSIKGG
                     KQVVLMADDDIQAKASHL"
BASE COUNT          701 a          632 c          627 g          585 t
ORIGIN      
        1 cgtgcattat tcggtgcccg taaaaaaatg gctgttttct tttaatcaca atggacatcg
       61 ttaccacgaa gcaaccgaag gctattccgt caattacgat tacaacggca aacaatatca
      121 gagcagcctg gccgccgagc gcatgctttg gcgtaacaga cttcataaaa cttcagtcgg
      181 aatgaaatta tggacacgcc aaacctataa atacatcgac gatgccgaaa tcgaagtgca
      241 acgccgccgc tctgcaggct gggaagccga attgcgccac cgtgcttacc tcaaccgttg
      301 gcagcttgac ggcaagttgt cttacaaacg cgggaccggc atgcgccaaa gtatgcctgc
      361 accggaagaa aacggcggcg atattcttcc aggtacatct cgtatgaaaa tcattactgc
      421 cggtttggac gcagccgccc catttatttt aggcaaacag cagtttttct acgcaaccgc
      481 cattcaagct caatggaaca aaacgccgtt ggttgcccaa gataaattgt caatcggcag
      541 ccgctacacc gttcgcggat ttgatgggga gcagagtctt ttcggagagc gaggtttcta
      601 ctggcagaat actttaactt ggtattttca tccgaaccat cagttctatc tcggtgcgga
      661 ctatggccgc gtatctggcg aaagtgcaca atatgtatcg ggcaagcagc tgatgggtgc
      721 agtggtcggc ttcagaggag ggcataaagt aggcggtatg tttgcttatg atctgtttgc
      781 cggcaagccg cttcataaac ccaaaggctt tcagacgacc aacaccgttt acggcttcaa
      841 cttgaattac agtttctaac ctctgaattt tttactgata tttagacggt ctttccttat
      901 cctcagaccg tcaaacttta cctacgtact tggcgcgcag tacgttcatc ttcaaaatgg
      961 aatagacatg aataaaggtt tacatcgcat tatctttagt aaaaagcaca gcaccatggt
     1021 tgcagtagcc gaaactgcca acagccaggg caaaggtaaa caggcaggca gttcggtttc
     1081 tgtttcactg aaaacttcag gcgacctttg cggcaaactc aaaaccaccc ttaaaacctt
     1141 ggtctgctct ttggtttccc tgagtatggt attgcctgcc catgcccaaa ttaccaccga
     1201 caaatcagca cctaaaaacc agcaggtcgt tatccttaaa accaacactg gtgccccctt
     1261 ggtgaatatc caaactccga atggacgcgg attgagccac aaccgctata cgcagtttga
     1321 tgttgacaac aaaggggcag tgttaaacaa cgaccgtaac aataatccgt ttctggtcaa
     1381 aggcagtgcg caattgattt tgaacgaggt acgcggtacg gctagcaaac tcaacggcat
     1441 cgttaccgta ggcggtcaaa aggccgatgt gattatcgcc aaccccaacg gcattaccgt
     1501 gaacggcggc ggctttaaaa acgtcggccg cggtatctta accaccggta cgcctcaaat
     1561 cggcaaagac ggtgccttga cgggatttga cgtgcgccaa ggcacattga ccgtcggtac
     1621 gtcaggttgg aacgacaaag gcggagccga ttacaccgaa gtgcttgccc gtgcagttgc
     1681 tttgcagggg aaattacagg gtaaaaacct ggcggtttct atcggtcctc agaaagtaga
     1741 ttacgccagc ggcgaaatca gtgcaggtac ggcagcgggt acgaaaccga ctattgccct
     1801 tgatactgcc gcactgggcg gtatgtacgc agacagcatc acactgattg ccaatgaaaa
     1861 aggcgtaggc gtcaaaaatg ccggcacact cgaagcggcc aagcaattga ttgtgacttc
     1921 gtcaggccgc attgaaaaca gcggccgcat cgccaccact gccgacggca ccgaagcttc
     1981 accgacttat ctctccatcg aaaccaccga aaaaggagcg gcaggcacat ttatctccaa
     2041 tggtggtcgg atcgagagca aaggcttatt ggttattgag acgggagaag atatcagctt
     2101 gcgtaacgga gccgtggtgc agaataacgg cagtcgccca gctaccacgg tattaaatgc
     2161 tggtcataat ttggtgattg agagtaaaac taatgtgaac aatgccaaag gctcggctaa
     2221 tctgtcggcc ggcggtcgta ctacgatcaa tgatgctact attcaagcgg gcagttccgt
     2281 gtacagctcc accaaaggcg atactgaatt gggtgaaaat acccgtatta ttgctgaaaa
     2341 cgtaaccgta ttgtccaacg gcagtattgg cagtgctgct gtaattgagg ctaaagacac
     2401 tgcacacatt gaagcgggta aaccgctttc tttagagact tcaaacgttg cttctaacat
     2461 ccgtttgaac aacggtagca ttaaaggcgg aaagcaggtg gtattgatgg ctgatgacga
     2521 cattcaggca aaagcctccc acctg
//
LOCUS       DAXHRI010000204         2539 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXHRI010000204 DAXHRI010000000
VERSION     DAXHRI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.204"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(1361..1798)
                     /gene="rnhA"
                     /locus_tag="ACFM75_001990"
     CDS             complement(1361..1798)
                     /gene="rnhA"
                     /locus_tag="ACFM75_001990"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC6256459.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRHCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1912..2301
                     /locus_tag="ACFM75_001991"
                     /pseudo
     CDS             1912..2301
                     /locus_tag="ACFM75_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
BASE COUNT          516 a          643 c          687 g          693 t
ORIGIN      
        1 tttaatttgt ctgtccgcat tgccgttaat tgtaccactt catcgtgttt ccttttcggt
       61 tgaaacccct ccctttgtgg cggcaggaac agactttatt tgggaggggt gtagcccctt
      121 ccgaatcagg acgacacata gggcggtgct ttatgtgtcg tcctgtgtgt tgaaacatat
      181 cggacgcgtt tgaacagttg tgcggcaaac tgttttagcg gcaggcaaga gatgccgtct
      241 gaagcccatt gtccgtcatt cccacacagg cagttgctac ggttgctgaa gccccttagc
      301 cgtcgcgcag gcggttgcta cggttactaa aatcccgtca ttcccgcgca ggcgggaatc
      361 cagtccgttc ggtttcagtt gtttctgata aatcttgcag cgttgggttt ttagattccc
      421 gctttcgcgg gaatgacggg gcggtcggat gccgcctgaa actccgtcat tcttgcaact
      481 tttcgtcatt cccgtgaaaa cgggaatcta gaacttcaaa ctttcagata atctttgaat
      541 attgccgttg cccgacggtt tggattcccg cttgcgcggg aatgacggta gagtggtttc
      601 agttgctccc gataaatgcc gcaatctcaa atcccgtcat tccctcaaaa acagaaaaac
      661 aaaatcagaa acctaaaatc ccgtcattcc cgcagctctt cgtcattccc gtgaaagcgg
      721 gaatctagac cttttaaatt ttcacacggc ttttgaatat tgccgttacc cgaaggtctg
      781 gattcccgcc tgcacgggaa tgacggcaga gcggtttcag ttgtttctaa ataaattttt
      841 gcagcgttgg atttttagat tcccgctttc gcgggaatga ctgggcggtc ggatgccgcc
      901 tgaaactccg tcattcctgc aacttttcgt cattcccgtg aaaatgggaa tctagaactt
      961 caaactttca gataatcttt gaatattgcc gttacccgac ggactggatt cccgcctgca
     1021 cgggaatgac ggcagagcgg ttttagttgt ttctaaataa atttttgcag cgttggattt
     1081 ttagattccc gctttcgcgg gaatgacggg gcggtcggat gccgcctgaa gcccgacaaa
     1141 gcccccaatg ccgtctgaaa cctcgtcatt cctctgctgt ccgcaacttt tcgtcattcc
     1201 cgcaaaagcg ggaatctaga gcctctaaat tttcagacgg cttttgaata ttgccgttgc
     1261 ccgacggttt ggattcccgc ctgcgcggga atgacggtgg aggacgatgc cgtctaaagc
     1321 ccattagcgg tttcagacgg catttttgcc ggagcggcag tcaggaaaac tgcgctgcgc
     1381 cacggtttgc caaatcgtcg gcgcgttcgt tttccgcgtg tcccgcgtgt cctttaaccc
     1441 aagtccagct gacttgatgc cgtccgacca gagcgtcgag ttctttccat aagtcgtcgt
     1501 ttttgacggg ctgtttggcg gcggttttcc agccgttgcg cttccaaccg tgtatccagt
     1561 tttccatgcc gtttttaacg tattgcgagt cggtgcagat gatgacggtg cagtggcgtt
     1621 tgagggattt cagtccttcg atgacggcag tcagttccat gcggttgttg gtggtttgcg
     1681 cttcgccgcc gaaaagttct ttttcgtggc taccgtagcg cattaacacg ccccagccac
     1741 ccgcgccggg attgcctttg cacgcgccgt cggtgtaaag gtaaacggtt tggtccatta
     1801 ttgtgccttt gtgtggtgtc cggcgtgatt ataagcggtt tgtgcgccgt gccgtctgaa
     1861 agattgcggc ggattaaaat aggcatataa tgtgcaactt tatttttgaa tatgaaggag
     1921 cggatagtgg gtcaaagcgg cgagttgttt tgtttcggac aaatgcccgt atggaaggcg
     1981 gaaaacctgc cggaaatttt gttgtcgggt tattcgtccg aaggagggga atgggtctgc
     2041 ctgaatgtgt tgcagggcga tgtcgaagtc cgcacgcagg acggggcggc ggaggtttgg
     2101 tcggcagaaa gcggcgattg cgtgtttgcg ccgcagcagg tgttttcggt caaactgaaa
     2161 acggacgatg ccgaaatccg tttgtcgctg tattgcgcgg cggcagacta ttttcacaaa
     2221 aaatacggga tgagtgccac gcattctgcg gtcgcggcgg cacgggatac cgtaccggcg
     2281 ggcagggcgt tggatatggg ttgagacctt tgcaaaattc ctttccctcc cgacagccga
     2341 aacccaaaca caggttttcg gctgtttccg ccccagatac ctcctaattc tacccaaacg
     2401 cccccttaat cctccccgga tacccgataa tcaggcatcc gggctgcctt ttaggcggca
     2461 gcgggcgcac ttagcctgtt ggccgctttc aacaggttca aacacatcgc tttcaggtgg
     2521 ctttgcgcac tcactttga
//
LOCUS       DAXHRI010000205         2538 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXHRI010000205 DAXHRI010000000
VERSION     DAXHRI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.205"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..768)
                     /locus_tag="ACFM75_001992"
     CDS             complement(<1..768)
                     /locus_tag="ACFM75_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6256460.1"
                     /translation="MNVQKSTRFIGIKVGKSNYSKNELNETKLPVRVIAQTAKTRSGW
                     DTVLEGTEFKTTLSGADIQAGVGEKARADAKIILKGIVNRIQTEEKLESNSTVWQKQA
                     GSGSTVETLKLPSFEGPALPKLTAPGGYIADIPKGNLKTEIEKLAKQPEYAYLKQLQT
                     VKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVAAVTGGVGAGLIGAAQGTASAAV
                     ADAAFTALVSQASISLINNKGNIGNTLKELGRSRTVKN"
     gene            complement(914..1177)
                     /locus_tag="ACFM75_001993"
     CDS             complement(914..1177)
                     /locus_tag="ACFM75_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256461.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(1196..>2538)
                     /locus_tag="ACFM75_001994"
     CDS             complement(1196..>2538)
                     /locus_tag="ACFM75_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6256462.1"
                     /translation="TEAGAAIIALAVTVVTSGAGTGAVLGLNGAAAAATDAAFASLAS
                     QASVSFINNKGDVGKTLKELGRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWINN
                     LTVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVHK
                     IAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALVKNTDFSRMSATEIEKAKANITA
                     YAKLVAGATVGVTGGNVDVAANASETAVKNNALDIIWDIGNLVWDGGKWIYAKSIGDK
                     QMAREAAIDFGVDAAAAAVPFVPAGATKISRGGAYVLKAGDEAVDTAKAIQEIQKQTG
                     IKLTYDKVNKVWTTPAGLDYGLDAKHGNRIKHVLAHTIPNPNKPVHSVFNVSRKEVLP
                     LVDEAWRMKGNPLPNDSSVYLVDMKKPIGTKGETKVRIVVQKGTNKIISAYPQK"
BASE COUNT          556 a          656 c          557 g          769 t
ORIGIN      
        1 atttttcacc gtgcggcttc tgcccagctc tttcagggtg ttaccgatat tgcctttgtt
       61 gttgatcagg gaaatggaag cctggctcac caatgcggta aaggcagcat cggccacggc
      121 tgccgatgcg gtaccctgtg ccgctccgat aaggcctgcg cctactcctc cggttaccgc
      181 cgctactgcc agtgcgataa ttgcggctcc ggctccggtt aggccttcct gtttatagtc
      241 ccatttgtcg taagcgagct gtacttggtt ccagttcacg tccttgaccg tctgaagctg
      301 tttcagatag gcatattcgg gctgtttggc cagcttttcg atttcggttt tgaggttgcc
      361 tttggggatg tcggcgatat agccgccggg agcggtcagc ttaggcagtg ccggcccttc
      421 aaagctcggt agcttcagcg tttcaaccgt gctgccgctt ccggcctgct tttgccatac
      481 ggtcgagttg gattccagct tttcttcggt ttggatgcgg ttaacgatgc cttttaggat
      541 aattttcgca tcggctcggg ctttttcacc cacccctgcc tgtatgtcgg ctccggaaag
      601 ggtggttttg aattcggtgc cttcgagtac ggtatcccag ccggaacggg ttttggctgt
      661 ttgggcgata acgcgtacgg gcagtttggt ttcgttcagc tcgtttttgc tgtaattgct
      721 tttacccact ttgatgccga tgaaacgggt acttttctga acattcaatt ggtgttggtg
      781 aatgccttcg gctgccagca gttgcattga ggccgtctga aaaacggata ttcagacggc
      841 ctattgctac tttaaataat atttgggttt aaaatatcat cgtatttccc aaattcgggg
      901 aacagaaaat caattattta aatttttcat cctcataata tgggtgatca aaaagctctc
      961 cttcccacat atttatttct tctatgcctt tatctttcat aaactcatga taatcataaa
     1021 aatcttttcc atattgtccg taaaaaattt tcatatcccc aagccacata gcataacatt
     1081 catcgcataa aatgacctcc ccagagaaat atttaggctt tgcatgataa aggtagcctt
     1141 gctcacatct tgggcaaatt ttaatttctt tatccataag agattccttt cttaattatt
     1201 tctgaggata tgcagaaatg attttatttg ttcctttttg cacaacaatc cgcacttttg
     1261 tttctccttt tgttccaata ggtttcttca tatctacaag atatacggat gaatcatttg
     1321 gcaaaggatt tcctttcatt ctccaagctt catcaaccaa aggcaaaact tctttacggg
     1381 acacattaaa aacagaatga acaggtttgt ttggatttgg aattgtatgg gctaaaacat
     1441 gtttaatcct attaccatgc ttagcatcta acccataatc taaccccgcc ggtgttgtcc
     1501 aaaccttatt aaccttatca taagtaagct tgattccggt ctgcttctga atttcctgta
     1561 tggctttagc cgtatcaact gcttcgtctc ccgccttcag aacataagcc ccgcctcggc
     1621 tgattttagt cgcacctgcc ggaacaaagg gaacggcagc tgcggcggca tccacaccaa
     1681 aatcaatcgc cgcttctcga gccatctgct tatcgccaat agatttggcg taaatccatt
     1741 taccgccgtc ccatacgagg ttgccgatat cccaaataat atctaatgca ttatttttaa
     1801 cagctgtttc ggaagcattt gccgccacat caacattgcc tcctgtaaca cctacagtcg
     1861 ctccggctac caattttgca tacgcagtaa tattcgcttt agctttttcg atttcggtcg
     1921 cactcatacg actgaaatca gtattcttaa ccaaagcctc accaacaatc tcaccgactg
     1981 cagcgcctat cgcgccatcc tgacacttgc ccttattcgc cgccgcagcc gcacagcccg
     2041 ctatggcatg ggcaatcttg tggactatgt agtgctgatc caactgtttg attttactgg
     2101 ctgcttctcc atgcgcggta ttgaccaaag ccgcaaggat attcgcttcc agattgtctt
     2161 tcaggctgcc gccgttgaca gcggtattaa tcagtgcggc actgcccgca ttggccaggt
     2221 tgacggtcag gttgttgatc cactgcttat cgctgacatt gttcagtgcc gaagcgccga
     2281 ttttgtcggc tacgcctgcg gtagcggcgg caaccaccag atttttcacc gtgctgcttc
     2341 tgcccagctc tttcagggtt ttgccgacat cgcctttgtt gttgatgaac gatacggaag
     2401 cctggctggc caaagaggca aatgctgcat cggttgcggc ggcggccgca ccgtttaatc
     2461 ccaatacggc tccggttcct gcgcctgagg tgaccacggt aacggccagt gcgataatcg
     2521 ccgcacctgc ttcggtta
//
LOCUS       DAXHRI010000206         2485 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXHRI010000206 DAXHRI010000000
VERSION     DAXHRI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.206"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(13..819)
                     /locus_tag="ACFM75_001995"
     CDS             complement(13..819)
                     /locus_tag="ACFM75_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC6256463.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQVMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILNR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="ACFM75_001996"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="ACFM75_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC6256464.1"
                     /translation="MLDNLTGRFSNVFKNIRGQVKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALTELMGRENKTLDLSVSPPAI
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
BASE COUNT          682 a          626 c          643 g          534 t
ORIGIN      
        1 tgttggctct tcttatctgt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcatcagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 tgttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg acctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 gcgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagcgat gtgccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcgaat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 catcagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccgaccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtctgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccgaca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggtcaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt cgtcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagtg gcgggcagcc tgacgcctga ccaagccttt
     1261 atcggcgtgg tcaaccaagc cttgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttt caccgcctgc cattgttttg atggccggtt tgcagggtgc cggtaagaca
     1381 acaaccgtcg gcaaactcgc ccgcctgctg aaaaacgatc agaagaaaaa agttttggtg
     1441 gtatccgccg acgtttaccg ccctgccgcg attgaacaac tgcgtctgtt ggccgaacag
     1501 gtcggcgtgg actttttccc atccgatacc aaccaaaaac cggttgagat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgtattga tggtcgatac cgccggccgt
     1621 ttggcaatcg atgaggagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccggt gtcgtattga ccaagatgga cggcgactcg
     1801 cgcggcggtg cggcattgtc cgtacgccat gtgaccggca aaccgattaa atttatcggc
     1861 gtcggcgaga aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cagccgtatc
     1921 ctcggcatgg gcgacgtatt gaccctgatt gaagacgttc aaaaaggtat agacgaagaa
     1981 gccgccgcca aaatggcgaa aaagctgcac aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccaacagat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat atcgaaacaa attcccgaag gcacggctga aaaagcgatg
     2161 ggcaaagtag aagccatcat caactcgatg acccctaaag aacgcgccaa ccctgccctg
     2221 ctcaaagccg gccgcaaacg ccgtattgca atgggtgcgg gcacaaccgt gcaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacagatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaggg ggataaaagg gatgttcccg
     2401 ggtttgtaag ccgatttaac agaaaacgcc gtctgaaaag tttcagacgg cgtttttgtt
     2461 ttatattctg atttatagtg gatta
//
LOCUS       DAXHRI010000207         2481 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXHRI010000207 DAXHRI010000000
VERSION     DAXHRI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.207"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            419..494
                     /locus_tag="ACFM75_001997"
     tRNA            419..494
                     /locus_tag="ACFM75_001997"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            879..1044
                     /locus_tag="ACFM75_001998"
                     /pseudo
     CDS             879..1044
                     /locus_tag="ACFM75_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
     gene            complement(1094..1900)
                     /gene="thiD"
                     /locus_tag="ACFM75_001999"
     CDS             complement(1094..1900)
                     /gene="thiD"
                     /locus_tag="ACFM75_001999"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC6256465.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCEAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            2344..>2481
                     /locus_tag="ACFM75_002000"
     CDS             2344..>2481
                     /locus_tag="ACFM75_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6256466.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDRVIDWQPIEQYLNRQKTR
                     YL"
BASE COUNT          600 a          642 c          626 g          613 t
ORIGIN      
        1 aggttctaga ttcccgcttt cgcgggaatg acgaaaagtg gcgggaatga cgaagtttca
       61 gacggcatcg cccgcctgtt ttgatatggc ggcacccccc cgacaaaaaa aacaatccgg
      121 aacgcatctg accgttccgg cttgttttca ggcgaatccg ccgcatcaga acatactgcg
      181 cacgcccata ttgacctgcc aagtctggcg catcgtgtgc atcgaagacc tttgcgcctc
      241 aaaataaagc tgccttccgt tgtcggcatt accacgcaaa aaaatgaatt gattgatatt
      301 ccaatgtttt ttatatgttt ttatattgtg atgcgatcag acaaacgccc ccctgacatt
      361 tgtttagacg gcatcgtatt gctaaatttc tataagtatg tataatgtcc gtttccacgc
      421 gcccatcgtc tagaggccta ggacactgcc ctttcacggc ggcaaccggg gttcgaatcc
      481 ccgtgggcgt gccaattcaa aaacctgctt gtttcaagca ggttttttta ttatgaatcg
      541 tcattcccgc acccttcgtc attcccgcga aagcgggaat ctggagcgca ggattaaaga
      601 aaccgtttta tccgataagc ttccgtgccg acaggtctgg attcccgcct gcgcgggaag
      661 gacggcagag ggtggacgat gccgtctaaa gcctgacaag gcatttgatg ccgtctgaaa
      721 cttcgtcatt cccgcaccct tcgtcattcc cgcaaaagcg ggaatctaga gcgtagggtt
      781 gaagaaaccg ttttatccaa taagtttccg tgccgacagg tctggattcc cactttcgtg
      841 ggaatgacga gtcttttata acctgagacc tttgcaaaat gcctttccct tttaaattta
      901 aagagggggg agctgaagga ttattatcgg gattgggagc tggtcgaata taaagaagaa
      961 ttgggcgcga tgcacgccaa agacgaaaac ggcaatccga tacggtttaa atttgtaacc
     1021 atgctggcga aaaagcctga ataatcctca aaaatgccgt ctgaacattt tgtttcagac
     1081 ggcattttta cgtctagtcc cgatacgccc aatgattgac cgggccgtgt cccgcgccga
     1141 tttccaaagg gtttgagatt gccgccgtaa tgtaggactt ggcagtccgt acggcttcgc
     1201 aaacgtccga gccttttgcc aactcggcgg tgatgcaggc ggaaaacgtg cagcccgtgc
     1261 cgtgcgtgtg ggcggttgga aagcgcgggc tgtccaattc cagcgtttca ttttgcgtaa
     1321 acagccaatc cgtgcagcgt ccgcttgtgc tgccgttcaa atgtccgcct ttgataacga
     1381 catttttgac accgtaatca agcaggattt ttgccgcacg ttccgcatct ttacggtttt
     1441 cgatatgcac gccggtcagg gcttccgcct cgggcaggtt gggggtcaat acatccgtat
     1501 cgggaagcag caggcgcgtc agtgccgcaa cggcggaatc ctgcaacagc ggcgcaccgc
     1561 ctttggcaat catcacaggg tcgagtacgc gcctgccgaa gtcgcagtgt ttcagcttgt
     1621 cggcaacgca ttcgatgatt tccgccgtgc cgagcattcc tattttgtag gcgcggatgt
     1681 caaagtcttc cctgattgct tggatttgtg cggtgatggt ttcggtcggg acgagatgaa
     1741 ccgccgacac gcccaaggta ttttgcgcgg taacggcggt gatgacgcac gttccgaaca
     1801 cgccgcgcat ctgaaacgtt ttcaaatccg cctgaatgcc cgcaccgccg cccgaatccg
     1861 aaccggcgat ggtaagcgtt tgcacaaaag agcttttcat ttgttcctcc ttaatattgt
     1921 gtccgataaa aagggctaat gcctgtaaaa aagcacggcg atgccgtctg aaaaccgcag
     1981 cgcggggttt gtggtgcatt atatgccgta ttgcaggaca aggcacaggg cagggcggcg
     2041 gggtgcatcg tagcaactgt atttttgccc gacggggtga aaaatacagt tgctacaccc
     2101 actgataatg aaaaaagaga gcaatgccgt ctgaaccgcg tgttgcggcg agcataaacc
     2161 ggcacgcttt attcgtcatt cccacaaaaa cagaaaccta aaattccgtc attcctacga
     2221 aagcgggaat ccagtgtgtt cggtttcagt tgtttttggg tttcgggtga tttccaaatc
     2281 gaaacctttg caaaattccc caaaatcccc taaattccca ccaagacatt taggggattt
     2341 ctcatgagca ccttcttcca acaaaccgcc caagccatga ttgccaaaca catcgaccgt
     2401 ttcccgctat tgaaattgga ccgggtgatt gattggcagc cgatcgaaca atacctgaat
     2461 cgtcaaaaaa cccgttacct c
//
LOCUS       DAXHRI010000208         2473 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXHRI010000208 DAXHRI010000000
VERSION     DAXHRI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.208"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>2473
                     /locus_tag="ACFM75_002001"
     CDS             <1..>2473
                     /locus_tag="ACFM75_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6256467.1"
                     /translation="HTGKDLNLNVDKDLSAASIHLKSDNAAHITGTSKTLTASKDMGV
                     EAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTALQGNIVSDGLHAV
                     SADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNITSSSGDITLVAGN
                     GIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSDRALSIENTKLES
                     THNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLVANGVLALNARYS
                     QIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPLAGRLNIEAGSGT
                     LTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKGNIRLVTGETDLR
                     GSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKELEQQIAQLKKSS
                     PKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQNIDLISAQGIEIS
                     GSDITASKKLNLHAAGVLPKAADSESAAILIDGITDQYEIGKPTYKSHYDKAALNKPS
                     RLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLEAGQNDAYTFLKT
                     KGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTTRFNAPAGKVTLV
                     AGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKLPVRVVAQTAATR
                     SGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKGIVNRIQSEEKLETNSTVWQ
                     KQAGRGSTIETLKLPSFESPTPPKLS"
BASE COUNT          779 a          648 c          549 g          497 t
ORIGIN      
        1 ttcatacagg taaagatctg aatttgaatg ttgataaaga tttgtctgcc gccagcatcc
       61 atttgaaatc ggataacgct gcccatatta ccggcaccag taaaaccctc actgcctcaa
      121 aagacatggg tgtggaggca ggctcgctga atgttaccaa taccaatctg cgtaccaact
      181 cgggtaatct gcacattcag gcagccaaag gcaatattca gcttcgcaat accaagctga
      241 acgcagccaa ggctctcgaa accaccgcat tgcagggcaa tatcgtttca gacggccttc
      301 atgctgtttc tgcagacggt catgtatcct tattggccaa cggtaatgcc gactttaccg
      361 gtcacaatac cctgacagcc aaggccgatg tcaatgcagg atcggttggt aaaggccgtc
      421 tgaaagcaga caataccaat atcacttcat cttcaggaga tattacgttg gttgccggca
      481 acggtattca gcttggtgac ggaaaacaac gcaattcaat caacggaaaa cacatcagca
      541 tcaaaaacaa cggtggtaat gccgacttaa aaaaccttaa cgtccatgcc aaaagcgggg
      601 cattgaacat tcattccgac cgggcattga gcatagaaaa taccaagctg gagtctaccc
      661 ataatacgca tcttaatgca caacacgagc gggtaacgct caaccaagta gatgcctacg
      721 cacaccgtca tctaagcatt accggcagcc agatttggca aaacgacaaa ctgccttctg
      781 ccaacaagct ggtggctaac ggtgtattgg cactcaatgc gcgctattcc caaattgccg
      841 acaacaccac gctgagagcg ggtgcaatca accttactgc cggtaccgcc ctagtcaagc
      901 gcggcaacat caattggagt accgtttcga ccaaaacttt ggaagataat gccgaattaa
      961 aaccattggc cggacggctg aatattgaag caggtagcgg cacattaacc atcgaacctg
     1021 ccaaccgcat cagtgcgcat accgacctga gcatcaaaac aggcggaaaa ttgctgttgt
     1081 ctgcaaaagg aggaaatgca ggtgcgccta gtgctcaagt ttcctcattg gaagcaaaag
     1141 gcaatatccg tctggttaca ggagaaacag atttaagagg ttctaaaatt acagcaggta
     1201 aaaacttggt tgtcgccacc accaaaggca agttgaatat cgaagccgta aacaactcat
     1261 tcagcaatta ttttcctaca caaaaagcgg ctgaactcaa ccaaaaatcc aaagaattgg
     1321 aacagcagat tgcgcagttg aaaaaaagct cgcctaagag caagctgatt ccaaccttgc
     1381 aagaagaacg cgaccgtctc gctttctata ttcaagccat caacaaggaa gttaaaggta
     1441 aaaaacccaa aggcaaagaa tatctgcaag ccaagctttc tgcacaaaat attgacttga
     1501 tttccgcaca aggcatcgaa atcagcggtt ccgatattac cgcttccaaa aaactgaacc
     1561 ttcacgccgc aggcgtattg ccaaaggcag cagattcaga gtcggctgct attctgattg
     1621 acggcataac cgaccaatat gaaattggca agcccaccta caagagtcac tacgacaaag
     1681 ctgctctgaa caagccttca cgtttgaccg gacgtacggg ggtaagtatt catgcagctg
     1741 cggcactcga tgatgcacgt attattatcg gtgcatccga aatcaaagct ccctcaggca
     1801 gcatagacat caaagcccat agtgatattg tactggaggc tggacaaaac gatgcctata
     1861 ccttcttaaa aaccaaaggt aaaagcggca aaatcatcag aaaaaccaag tttaccagca
     1921 cccgcgacca cctgattatg ccagcccccg tcgagctgac cgccaacggt atcacgcttc
     1981 aggcaggcgg caacatcgaa gctaatacca cccgcttcaa tgcccctgca ggtaaagtta
     2041 ccctggttgc gggtgaagag ctgcaactgc tggcagaaga aggcatccac aagcacgagt
     2101 tggatgtcca aaaaagccgc cgctttatcg gcatcaaggt aggtaagagc aattacagta
     2161 aaaacgaact gaacgaaacc aaattgcctg tccgcgtcgt cgcccaaact gcagccaccc
     2221 gttcaggctg ggataccgtg ctcgaaggta ccgaattcaa aaccacgctg gccggtgccg
     2281 acattcaggc aggtgtaggc gaaaaagccc gtgccgatgc gaaaattatc ctcaaaggca
     2341 ttgtgaaccg tatccagtcg gaagaaaaat tagaaaccaa ctcaaccgta tggcagaaac
     2401 aggccggacg cggcagcact atcgaaacgc taaaactgcc cagcttcgaa agccctactc
     2461 cgcccaaact gtc
//
LOCUS       DAXHRI010000209         2469 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXHRI010000209 DAXHRI010000000
VERSION     DAXHRI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.209"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..475
                     /locus_tag="ACFM75_002002"
     CDS             <1..475
                     /locus_tag="ACFM75_002002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256468.1"
                     /translation="PRHSRAGGNPETQRNRNLSEKTETPTASFPRRRESRNTTRQDFI
                     GKNRNPHHRHSRAGGNPKTQRNRNLSEKTETPPTVIPAKAGIQQHNAAGIYRKKQKPP
                     PTVIPAKAGIQQPKNHRNLSAKTETPTAVIPAKAGIQTRRHGNLPNKTVSLDSTS"
     gene            621..2402
                     /locus_tag="ACFM75_002003"
     CDS             621..2402
                     /locus_tag="ACFM75_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFC6256469.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVETAVLATLLF
                     ATVQASANNEEQEEDLDPVQRTVAVLIVNSDKEGTGEKVEENSDWAVYFNEKGVLKAG
                     TITLKAGDNLKIKQNTDENTDENTNASSFTYSLKKDLTDLTSVGTEELSFGANGNNKV
                     NITSDTKGLNFAKKTAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTDDEKKRAASVK
                     DVLNAGWNIKGVKPGTASDNVDFVHTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIG
                     AKTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQT
                     GQADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWN
                     LDSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSS
                     VSLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNL
                     NNRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDG
                     GNWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          791 a          625 c          604 g          449 t
ORIGIN      
        1 accgcgtcat tcccgcgcag gcgggaatcc agaaacgcaa cgcaacagga atttatcgga
       61 aaaaacagaa acccccaccg cgtcattccc gcgcaggcgg gaatccagaa acacaacgcg
      121 gcaggacttt atcggaaaaa acagaaaccc tcaccaccgt cattcccgcg caggcgggaa
      181 tccaaaaacg caacgcaaca ggaatttatc agaaaaaaca gaaaccccac cgaccgtcat
      241 tcccgcaaaa gcgggaatcc agcaacacaa cgcagcagga atttatcgga aaaaacagaa
      301 acccccaccg accgtcattc ccgcaaaagc gggaatccag caaccgaaaa accacaggaa
      361 tctatcagca aaaacagaaa cccccaccgc cgtcattccc gcaaaagcgg gaatccagac
      421 ccgtcggcac ggaaacttac cgaataaaac agtttcctta gattccacgt cctagattcc
      481 cgccttcgcg ggaatgacga gattttaagt tgggggaatt tatcagaaaa cccccaaact
      541 cccaaaaacc gggcggatgc cgcaccatcc gcccccaaac cccgatttaa ccattcaaac
      601 aaaccaaaag aaaaaacaaa atgaacaaaa tataccgcat catttggaat agtgccctca
      661 atgcctgggt cgtcgtatcc gagctcacac gcaaccacac caaacgcgcc tccgcaaccg
      721 tggagaccgc cgtattggcg acactgttgt ttgcaacggt tcaggcaagt gctaacaatg
      781 aagagcaaga agaagattta gaccccgtac aacgcactgt tgccgtgttg atagtcaatt
      841 ccgataaaga aggcacggga gaaaaagtag aagaaaattc agattgggca gtatatttca
      901 acgagaaagg agtactaaaa gccggaacaa tcaccctcaa agccggcgac aacctgaaaa
      961 tcaaacaaaa caccgatgaa aacaccgatg aaaacaccaa tgccagtagc ttcacctact
     1021 cgctgaaaaa agacctcaca gatctgacca gtgttggaac tgaagaatta tcgtttggcg
     1081 caaacggcaa taataaagtc aacatcacaa gcgacaccaa aggcttgaat tttgcgaaaa
     1141 aaacggctgg gacgaacggc gacaccacgg ttcatctgaa cggtattggt tcgactttga
     1201 ccgatacgct gctgaatacc ggagcgacca caaacgttac cgatgacgag aaaaaacgtg
     1261 cggcaagcgt taaagacgta ttaaacgcag gctggaacat taaaggcgtt aaacccggta
     1321 cagcttccga taacgttgat ttcgtccaca cttacgacac agtcgagttc ttgagcgcag
     1381 atacgaaaac aacgactgtt aatgtggaaa gcaaagacaa cggcaagaga accgaagtta
     1441 aaatcggtgc gaagacttct gttatcaaag aaaaagacgg taagttggtt actggtaaag
     1501 gcaaaggcga gaatggttct tctacagacg aaggcgaagg cttagtgact gcaaaagaag
     1561 tgattgatgc agtaaacaag gctggttgga gaatgaaaac aacaaccgct aatggtcaaa
     1621 caggtcaagc tgacaagttt gaaaccgtta catcaggcac aaatgtaacc tttgctagtg
     1681 gtaaaggtac aactgcgact gtaagtaaag atgatcaagg caacatcact gttaagtatg
     1741 atgtaaatgt cggcgatgcc ctaaacgtca atcagctgca aaacagcggt tggaatttgg
     1801 attccaaagc ggttgcaggt tcttcgggca aagtcatcag cggcaatgtt tcgccgagca
     1861 agggaaagat ggatgaaacc gtcaacatta atgccggcaa caacatcgag attacccgca
     1921 acggcaaaaa tatcgacatc gccacttcga tgaccccgca attttccagc gtttcgctcg
     1981 gcgcgggggc ggatgcgccc actttaagcg tggatgacga gggcgcgttg aatgtcggca
     2041 gcaaggatgc caacaaaccc gtccgcatta ccaatgtcgc cccgggcgtt aaagaggggg
     2101 atgttacaaa cgtcgcacaa cttaaaggtg tggcgcaaaa cttgaacaac cgcatcgaca
     2161 atgtggacgg caacgcgcgc gcgggtatcg cccaagcgat tgcaaccgca ggtctggttc
     2221 aggcgtattt gcccggcaag agtatgatgg cgatcggcgg cggcacttat cgcggcgaag
     2281 ccggttacgc catcggctac tccagtattt ccgacggcgg aaattggatt atcaaaggca
     2341 cggcttccgg caattcgcgc ggccatttcg gtgcttccgc atctgtcggt tatcagtggt
     2401 aagggctttt tcgcctgtct gctgttggga caggcggaag gtttgaaggg aagggtggcg
     2461 atttgccgc
//
LOCUS       DAXHRI010000210         2437 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXHRI010000210 DAXHRI010000000
VERSION     DAXHRI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.210"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            41..2254
                     /locus_tag="ACFM75_002004"
     CDS             41..2254
                     /locus_tag="ACFM75_002004"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6256470.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRTVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILIDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          647 a          776 c          574 g          440 t
ORIGIN      
        1 gttacaataa actcgttttt atcggaacga aagacccatc atgaccgcca tcagcccgat
       61 tcaagacacg caaagcgcga ctctgcaaga attgcgcgaa tggttcgaca gctactgcgc
      121 cgcgctgccg gacaacgata aaaaacttgt cttagccgcc cgttcgctgg cggaagcaca
      181 ttaccccgcc gatgccgtca cgccgtatgg cgagccgttg tccgaccact tcctcggcgc
      241 ggcacaaatg gtcgacgaac tcgacctgct gcccgatgcc gtcgccgcca ccctgcttgc
      301 cgacatcgga cgctacgtcc ccgattggaa tctattggtt tccgaacgct gcaacagcac
      361 cgtcgccgag ctggtcaaag gtgtggacga agtgcagaaa ctcacccact tcgcccgggt
      421 ggacagcctc gccacgccag aagaacgcgc ccagcaggca gaaactatgc ggaaaatgct
      481 gctggcgatg gttaccgaca tccgcgtcgt gttaatcaaa ctggcgatgc gtacgcgcac
      541 cctgcaattc ttaagcaacg ttcccgacaa tcctgaaaag cgcaccgttg ctaaagaaac
      601 cctcgacatt tttgccccac tcgccaaccg cttgggcgtg tggcaactca aatggcagct
      661 cgaagatttg ggcttccgtc atcaagagcc ggaaaaatac cgcgaaatcg ccctgctttt
      721 ggacgaaaaa cgcaccgagc gcctcgaata catcgaaaac ttcctcaata tcctgcgtac
      781 ggaactcaaa aaatacaata tccactttga agtcgccggc cgtccgaaac acatctactc
      841 catttacaaa aaaatggtga agaaaaaact cagcttcgac ggcctctttg acatccgcgc
      901 cgtgcggatt ctgattgata ccgtccccga gtgttacacc acgctgggca tcgtccacag
      961 cctctggcag cccattcccg gcgagttcga cgactacatc gccaacccca aaggcaacgg
     1021 ctataaaagt ttgcacaccg tcatcgtcgg cccggaagac aaaggcgtgg aagtgcaaat
     1081 ccgcaccttc gatatgcacc aattcaacga attcggtgtc gccgcccact ggcgttacaa
     1141 agagggcggc aaaggcgatt ccgcctacga acagaaaatc gcctggttac gccaactttt
     1201 ggactggcgc gaaaacatgg cggaaagcgg caaggaagac ctcgccgccg ccttcaaaac
     1261 cgagcttttc aacgacacga tttatgtcct gaccccgcac ggcaaagtcc tctccctgcc
     1321 cacgggcgcg acccccatcg acttcgccta cgccctgcac agcagcatcg gcgaccgttg
     1381 ccgcggtgcg aaagtcgaag ggcaaattgt gccgctgtcc accccgctcg aaaacggaca
     1441 gcgcgtcgaa atcattaccg ccaaagaagg gcatccttcc gtcaactggc tttacgaagg
     1501 ctgggtcaaa tccaacaagg caatcggcaa aatccgcgcc tacatccgcc agcaaaacgc
     1561 cgacaccgtg cgcgaagaag gccgcgtcca actcgacaaa cagcttgcca aactcacgcc
     1621 caaacccaac ctgcaagagc ttgccgaaaa tctcggctac aaaaagccag aagacctcta
     1681 caccgccgtc ggacaaggcg aaatttccaa ccgcgccatc caaaaagcct gcggcacgct
     1741 gaacgaaccg ccgcccgtac ccgtcagcga aaccaccatc gtcaaacagt ccaaaatcaa
     1801 aaaaggcggc aaaaacggcg tgctcatcga cggcgaagac ggtctgatga ccacgcttgc
     1861 caaatgctgc aaacccgcgc cgcccgacga cattgtcggc ttcgttaccc gcgatcgcgg
     1921 catttcggta caccgcaaaa cctgcccctc tttccgacac ctcgccgaac acgcgcccga
     1981 aaaagtgctg gacgcaagct gggcggcgtt gcaggaagga caagtgttcg ccgtcgatat
     2041 cgaaatccgc gcccaagacc gctccgggct tttgcgcgac gtatccgacg cactcgcccg
     2101 ccacaaactc aacgttaccg ccgtgcaaac ccagtcccgc gacttggaag ccagcatgag
     2161 gttcacgctc gaagtcaaac aagtcaacga cctcccgcgc gtcctcgcca gcctcggcga
     2221 cgtcaaaggc gtattgagcg ttacccggct ttaaatacaa aaatgccgtc tgaaagccgt
     2281 ataacgcttc agacgacatt ttgattgccg gggtttgcta ttttttgttg cataatcaat
     2341 taaaaacaaa atagtacaat attcaacttt gaaggtctaa ccatggcata ctctgcggac
     2401 ttaagaaaca aagctttaaa ctatagtgga ttaaatt
//
LOCUS       DAXHRI010000211         2393 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXHRI010000211 DAXHRI010000000
VERSION     DAXHRI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.211"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            116..637
                     /locus_tag="ACFM75_002005"
     CDS             116..637
                     /locus_tag="ACFM75_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC6256471.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAVNTRGYSSKPIDTLMALA
                     NDGTIAGAKLVDHHEPIMLIGIPHLPAPGRAIRLNWLPAYIKPNFISTNRLRLKGLPT
                     VLQPSKGTGAGVP"
     gene            526..1725
                     /locus_tag="ACFM75_002006"
     CDS             526..1725
                     /locus_tag="ACFM75_002006"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC6256472.1"
                     /translation="MASGVYQTKLYIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSAEAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRF
                     AVHYMYTNDSEVSGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDGLLKEVETRQSEWGR
                     AENGSLN"
     gene            1783..>2393
                     /locus_tag="ACFM75_002007"
     CDS             1783..>2393
                     /locus_tag="ACFM75_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6256473.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRI"
BASE COUNT          591 a          658 c          633 g          511 t
ORIGIN      
        1 tatcccgtat gcggattggt tttaagattt gtaaatttga tttgcatcaa aaaatcgccg
       61 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
      121 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacggcgt tttttgccgc
      181 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
      241 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacggcaagc cggaaggtaa
      301 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
      361 cgatgcggtc aatacgcgcg gttattcgag caaaccgatc gatacgctga tggctttggc
      421 aaatgacggc acgatagccg gagcgaaact ggtcgatcat cacgaaccga ttatgctgat
      481 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc ttaaactggc ttccggcgta
      541 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc cgaccgttct
      601 gcaaccatcg aaggggacag gagcgggcgt accatagccg tacacgcgcc ggacgtaacg
      661 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc cagcaatgga tgctggtatt
      721 tatctcgaag aaaccgcccc gcgcgccctg attgaacaca acaatatttt ggataattcg
      781 gtcggcgtct atctgcatgg ttctgccgaa gcgatggtac gcgagaataa aatcgtcggc
      841 gatgcgactt tgcgcgtgaa cgagcgcggc aacggcgtta ccgtttggaa cgcgcccggc
      901 gcgcaggtcg tcggcaacga catttccaaa gggcgggatg gcattttttc caacaccagc
      961 acgcacaaca cctataaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
     1021 tacaccaacg acagcgaagt cagcggcaat atttccgtgg gcaacaatat gggctatgtg
     1081 ctgatgtttt ccgagcggct caaagtgttt gacaatatcg ccgtcggcag ccgcgatcag
     1141 ggcattatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaggcg
     1201 ggcaagtgcg tttttgccta caatgccaac tacgataaac tgtccgccaa tcattttgaa
     1261 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtccct gcatgacaat
     1321 tcctttatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
     1381 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
     1441 ttcggagaca gcgcgtaccg tcccaacggc atcatcgacc aaatcatctg gcgcgcaccc
     1501 gtatcgcgcc tcttgatgaa cagtcccgca atcagcatcg tcaaatgggc gcaggcgcag
     1561 tttcccgccg ttttgcctgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
     1621 cccaaaattc aaacccgtta tcaggcgatg aaggacgggc tactcaaaga agtcgaaacg
     1681 cggcagtcgg aatggggcag ggcggaaaac ggttctttga actagtctgc ttcagacggc
     1741 atccggattc aaatgccgtc tgaaaacaca aaaggaacaa ccatgaccac acatcatgtc
     1801 gaattgagga aggtaaccaa acgcttcggc gcgcaaaaag ccgtcaacca agtcgatttg
     1861 gttttgaagg caggagaaag cgtcgggctt gccggacaca acggtgcggg caagtccacc
     1921 atcatgaagc tgatactcgg gctgattacc ccgaccgaag gcgaagtgat gcttttgggc
     1981 gaacgtaccg gcagcaaagc gggggcgcgg cttcgcagcc aaatcggcta cctgcccgaa
     2041 accgttgcgc tgcacccttc gctgaccggc atcgaaacgc tggattttta tgccaaactt
     2101 aaaaaacagc cgctcacgca gaatcggggg ctgctcgagc gcgtcggcat ttcacaggcg
     2161 gcacaccgcc gcgtcggcac ttattctaaa gggatgcgcc aacgccttgc cttggcacaa
     2221 gccctgctgg gcgagcccaa agtcctgctg tttgacgaac cgacaaccgg tcttgaccct
     2281 gcatcaaggc aaatgtttta cgaagtcgtg cgcgaactca acgggcgcgg cgcgaccgta
     2341 ttgctcagca cccacgccct tgccgagttg gacggacacg ccgaccgcat tat
//
LOCUS       DAXHRI010000212         2309 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXHRI010000212 DAXHRI010000000
VERSION     DAXHRI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.212"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..568)
                     /locus_tag="ACFM75_002008"
     CDS             complement(<1..568)
                     /locus_tag="ACFM75_002008"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC6256474.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWNEVAK"
     gene            731..2137
                     /gene="dnaB"
                     /locus_tag="ACFM75_002009"
     CDS             731..2137
                     /gene="dnaB"
                     /locus_tag="ACFM75_002009"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC6256475.1"
                     /translation="MNDYTAMPSEDREIGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          570 a          588 c          615 g          536 t
ORIGIN      
        1 gtttggcgac ttcgttccag ttgacgattt cccaaaaacc tttcaggtag ttgggacggc
       61 tgttgcggta gtcgatgtaa taggcgtgtt cccacacgtc gcaggtcagc agcggcgtgt
      121 tttcagtggt cagcggcgta gcggcgttgg aagtagaaac caaatccaat ccgccggcag
      181 gggtttttac cagccacgcc caaccggagc cgaaagtacc ggccgcgcag gcattgaacg
      241 cttcttggaa tttctcgaag ctgccccatt tcgcgtcgat ggcggcggcc agttcgccgg
      301 caggtttgcc ttgacctttg gacgtgaaac ccagccagta gaaggtgtgg ttccaagttt
      361 gtgccgcgtt gttgaacacg ccgcccgaag attttttcac aatctcttcc aaaggcaggt
      421 tttcaaattc ggtgcctttg atttgattgt tcaggttggt gatgtaggtt tgatggtgtt
      481 tgccgtagtg gaattccaaa gtctctttgc tcagatgcgg ggacaatgcg tccagttcat
      541 aaggcagttg cggcagctta tgttccattt tgtgctccta atattgtttt aaatgttgta
      601 ttttggcagt gttgctgcaa atagctcagc agcgcacata ttttacctgt tttgagggat
      661 ggaaaccaat taaacctgct ttacgctata atagaagatt gcaatttcgg cacgacagat
      721 aggatgtacc atgaacgatt acacagccat gccgtctgaa gaccgtgaga tcggcgcatt
      781 gtcgctgcct ccacattcga tggaggcgga acaatccgtt ttgggcgggt tgatgctgga
      841 aaatccggca tgggacagga ttgccgatgt ggtttcggga gaggatttct accggcatga
      901 acaccgcctg attttccgat ccattgccaa attaatcaat gagagccgtc ccgccgatgt
      961 gattacggtt caggaagatt tgcagcggaa cgaagaattg gaagcggcag gcggatttga
     1021 atatctgatt acgctggcgc aaaacacccc gtctgccgcc aacatccgcc gctacgccga
     1081 aatcgtgcgc gagcgttcca ttatgcgcca actcgccgaa gtggggacgg aaatcgcccg
     1141 cagcgcatac aatccgcaag gcagggacgc gggtcagctt ttggacgagg cggaaaacaa
     1201 agtattccaa atcgccgaaa gcaccgccaa atccaagcag ggctttttgg agatgcccga
     1261 tttgctgaaa gaagtcgtac agcgcatcga tatgctctac tcgcgcgaca atcccgatga
     1321 agttaccggc gtgccgacag ggttcatcga cctcgacaaa aaaacctcgg gtctgcaacc
     1381 cggcgacctg attatcgttg ccggtcgtcc gtctatgggt aagaccgcct tttctatcaa
     1441 tatcgccgaa cacgttgccg tagaaggcaa gctgccggtt gccgtcttct caatggaaat
     1501 gggcggggcg caactggtca tgcgtatgct cggctcggtc ggacgcttgg atcaaagcgt
     1561 tttgaaaacc ggcaggctcg aagacgaaca ctggggtcgc ctgaacgaag cagtcgtcaa
     1621 actctccgac gcgcccgtgt acatcgacga gaccccgggt ctgaccgcgc tcgaactgcg
     1681 cgcccgtgcc cgccgtctcg cccgtcaatt taacaataag cttggattga ttgtcatcga
     1741 ctacctgcaa ctgatggcag gatccggccg ttccgacaac cgagcttcgg agctgggaga
     1801 gatttcacgt tcgctcaaag cgttggcgaa agaattgcaa gtccccatca tcgccctgtc
     1861 gcaattgagc cgcacggtcg aatcgcgtac cgacaaacgc ccgatgatgt ccgaccttcg
     1921 cgaatccggt gctatcgagc aggatgccga cctgattatg tttatgtacc gcgacgaata
     1981 ctacaaccaa gactcgccca tgaaaggcct tgccgaatgt atcatcggca aacaccgcaa
     2041 cggtcccgtc ggtaaaatct tcctcacatg gacgggacaa ttcaccaaat tcgacaatgc
     2101 tgcctatatt cccgaggagg caaagataga ggattaaatg gctatataaa aatttattag
     2161 gcgaaatcag gcaaaatcgt ttaaaatcat gctgagagat tgccctaaaa aataaaacgc
     2221 ggtcttgagg catttttgca ttcagcccgc atataattga aaatatagtg gattaacaaa
     2281 aatcaggaca aggcaacgaa gccgcagac
//
LOCUS       DAXHRI010000213         2305 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXHRI010000213 DAXHRI010000000
VERSION     DAXHRI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.213"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(264..695)
                     /locus_tag="ACFM75_002010"
     CDS             complement(264..695)
                     /locus_tag="ACFM75_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256476.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TITGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYYYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(711..1850)
                     /locus_tag="ACFM75_002011"
     CDS             complement(711..1850)
                     /locus_tag="ACFM75_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6256477.1"
                     /translation="MGEIVGEALTNGKNPATLTAKEREQILAYSKLVAGTVSGVVGGD
                     VNAAAKAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLA
                     ASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLA
                     LQSYHLNTAAKSWLQSGNTKPLSEWMADQGYTLISGVNPRFIPIPRGFVKQNTPITNV
                     KYPESISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEG
                     ITRIKYGIPTLDRTGKPDGGFKEISSIKTVYDPKKFSDDKILQMAQKAASQGYSTASK
                     IAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
     gene            complement(2030..2302)
                     /locus_tag="ACFM75_002012"
     CDS             complement(2030..2302)
                     /locus_tag="ACFM75_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256478.1"
                     /translation="MNKRMKICPTWQQGYLYHSKPKYLHDEIILCDECDAVWLKGVNI
                     FYGEYKKDFYSYVPFMESQGITGECIWEGDLFDHPYYEDENSNGMD"
BASE COUNT          627 a          461 c          409 g          808 t
ORIGIN      
        1 gaactgatat ccgtggtgct gagcaggatg cgactgttct cattggcata cggtgcgcta
       61 atgctcacac cggttttacc cgaaagttct gcagcaagcg tagggggtgt cttcagcacg
      121 cacgcgtctg ggcagtatat agggaatctg aatatttact tgcataacaa atgccgtctg
      181 aaaaattgtg agcttttcag acggcattga gccgtaaatc atggaacgcg tgcgtgctgg
      241 agcacacacc ttacgcatgg attttaggtt tcatgcaggc tacagtttgt ttgagaatat
      301 gttttttgta tttctttaca ctttttttga tattcagggt gagctattaa gaaatcagat
      361 attgcaatat taaagtaata atatgctaaa ttaataggta taattaattc ttcaggattg
      421 aaatcatctc caactgaaaa ataaatagaa ggagctttat caatatactc aaatccgttt
      481 acctcttcat aatctccaat ccatctggta gcatctattt tttcatattg agcacctatt
      541 actccaaaat gtccggtaat tgtaatattt ttaaaacact ctaaaagcgt atctttagtt
      601 tcaaattggt aaattgtttc aaaaaaaatc gaaataacaa aatggttgtt atttatagat
      661 tttttattta agtttagaaa aatattattt ttcataattt ttcctttaaa ttattctggg
      721 tgaatgtttg taattcttcc tgtatttaca tcaaaatatg atctaaattt gattccgtca
      781 aaggtttctg agaattgaat gacatttttt ctttccgata ttgatttagt tctttcattt
      841 tgagcaattt tagaggctgt tgaatatcct tgtgaagcag ctttttgagc catttgaagt
      901 attttatcat cagaaaattt tttaggatca taaacagttt ttatacttga aatttcctta
      961 aatccaccat caggtttacc tgtcctgtct agtgtaggaa tcccatattt aattcgggta
     1021 atgccttcaa tatcagtttg ggtttcagat tttacgcgtc ctcctcgtga atttagttct
     1081 gccataaaat tggtgcggtt atgggctcct ttaatgccct gtttttgact aaaaccatca
     1141 gcatttgcca gatgtctttt taggtttgta tcgaaactga tgctttccgg gtatttgaca
     1201 ttagtaatag gtgtattttg ttttacaaac cctcttggta ttggaatgaa tctaggatta
     1261 actcctgaaa taagtgtata accttggtcg gccatccatt cggataaagg ctttgtattg
     1321 cccgattgaa gccaagattt agcagcagta ttcaaatgat aagactgtaa agccaaatct
     1381 gcttgggtgt aagatttgct gaataattta taccattcat catctttacc aattagacct
     1441 gcttcccaag atgaatgaag gcttctacta tcgatcaaat gttgttttct gattgttcta
     1501 cattcagtac tacgggatat atccgtacat attgcaatcg aagcagcaag tcttttatca
     1561 gcaacatttt gatacttttt tacagtattt tttctgcaca gttgaggatt attctgtttg
     1621 gcgcatgcag tcatttcgtt atcaaattct ctaccctctt tgtcgctaag ctgattattt
     1681 ttcaccgcta cctcagccgc cttcgccgcc gcatttacat cgccgccaac cacaccgctt
     1741 accgtaccgg caaccagttt gctgtatgcc aaaatctgtt cgcgttcttt agctgtcaaa
     1801 gtggcaggat ttttgccgtt tgtcaaagcc tccccgacta tctcgcccac agccgcaccg
     1861 atcgcaccat cctgacactt gcccttattc gccgctgccg ccgcacagcc cgctacggca
     1921 tgggcaatct tgtgggtggt atatcgctgc cagccctctc tgttgatgat cttgcgtatg
     1981 attacgccgt ttgatggcta atcgtaccta cgcaggctta aagtttccat caatccatac
     2041 catttgagtt ttcatcttca taatatggat gatcaaacaa atctccttcc cagatacatt
     2101 cacccgttat accttgggat tccatgaaag gaacataaga ataaaaatct tttttatatt
     2161 ctccataaaa tatattcact cctttgagcc atactgcatc acattcatca cacagaataa
     2221 tttcatcatg aagatattta ggtttcgaat ggtagagata gccttgttgc caagtgggac
     2281 atattttcat tctcttattt atgat
//
LOCUS       DAXHRI010000214         2284 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXHRI010000214 DAXHRI010000000
VERSION     DAXHRI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.214"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            19..747
                     /gene="rpe"
                     /locus_tag="ACFM75_002013"
     CDS             19..747
                     /gene="rpe"
                     /locus_tag="ACFM75_002013"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC6256479.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(770..1801)
                     /gene="ruvB"
                     /locus_tag="ACFM75_002014"
     CDS             complement(770..1801)
                     /gene="ruvB"
                     /locus_tag="ACFM75_002014"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC6256480.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1883..2191)
                     /locus_tag="ACFM75_002015"
     CDS             complement(1883..2191)
                     /locus_tag="ACFM75_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256481.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          455 a          546 c          736 g          547 t
ORIGIN      
        1 gatattgaag gaaaaaacat gactacttac cgtatcgcac ccagcatttt atcggcagac
       61 tttgcccgcc tcggagaaga ggtggaaagc gtcattgcgg caggtgcaga tctgatccat
      121 tttgacgtga tggacaacca ttatgtgccg aacctgacct tcggccctat ggtttgcgcg
      181 gcgttgaagc cttatgcaag cgtgccgatt gatgtgcatc tgatggtcga acccgttgac
      241 gacctgattc agtcgtttgc caaagcagga gcatcaatca tcacgttcca tcccgaggcg
      301 agccgccata tcgaccgcag cttgagcctg attcgtgata tgggctgtca ggcggggctg
      361 gtgttgaatc cggcaacgcc cgtatatctg ttggaaaacg tattggacag gctggatatg
      421 gttttgctga tgtcggtcaa ccccggattc ggcggacaaa gcttcatccc gcacaccctt
      481 gaaaaaatcc gccgggtgcg ggcgatgctg gatcggtacg aagcacaaag cgggcggcac
      541 atcgccatcg aagtggacgg cggcatcaaa accgacaata ttgccgccgt tgcccgagct
      601 ggtgcggata cctttgttgc cggttctgcg attttcggca aaccagacta taaggcggtg
      661 attgatgcga tgagggcgga actggaaaaa agcggcaggc tgaaccttgc gccaatgatt
      721 gaaatagaaa tgccgtctga aaattagctc tgtttcagac ggcattgcgt tatttttcga
      781 cgggcagccc gaaatgcagg taggcgcgtt cggtcgccat cctgccgcgc ggggtgcgtt
      841 gcaggaagcc ttgttggata aggtagggtt cgataacgtc ttcgatggtg tctgtagatt
      901 cgccgatggc ggcggcaaca ttgtccaaac cgaccgggcc gccgccgaat ttgtgcaaaa
      961 cggcttcgag aaatttcctg tccatcacgt ccagcccctg cgcgtccacg tccagcatac
     1021 ttaaagcggc atcggcgatg ccgccgtcga ttgtgccgtt gtttttcacg tcggcgaaat
     1081 cgcgcacgcg tcgcaacagg cggttggcga tgcgcggcgt accgcggctg cgtttggcga
     1141 tttcttccgc gccttcttcg gacatatcga gctgcaacag ttgtgccgaa cggctgacga
     1201 tggtggtaag gtctcggttt tcgtaaaact caaggcggga gacgatgccg aagcggtcgc
     1261 gcaacggatt ggtcagcata ccggcgcggg tggtcgcgcc gatgagcgtg aagggcggca
     1321 ggtcgatttt gacggaacgg gcggcgggtc cttcgcctat cataatgtcg agccggtagt
     1381 cttcgagcgc gggatagagg atttcttcga caacagggct gaggcggtgg atttcgtcga
     1441 taaacaatac gtcgtgcgga tcaaggttgg tcaaaagggc ggcgaggtcg cctgcgcgtt
     1501 cgaggacggg gccgctggtt tggcgcaaat ttacgcccaa ttctttggcg atgatgtgcg
     1561 ccagtgtggt tttgcccagt ccgggcgggc cgaagagcaa aacgtggtcg agtgcttcgc
     1621 cgcgtttttt ggcggcttgg atgaaaatgg caagctgttc tttggctttg tcttgcccga
     1681 tgtagtcgtc cagcgttttg gggcggaggg cgcgttcgag cagttcttcc tgtgcggagg
     1741 cggtttgggc ggcaacgatg cgttgcggtt gcgcggcggt caggttgtcg gtttgcagca
     1801 tagtgttccc tttgtcgggt atgccgtccg aacggtcggc ggcgtttcag acggcattga
     1861 aaagataacg gtcaaaagcg ttttatccgc gggtttcgcg ccaaacgaga tagtcgcgca
     1921 ggggcggcat aggcgggttg atgcagtggg ggcagatgcc ggtggtgtct tcgtcgtcgc
     1981 tgtcgatgtg gtaggcgttg gggtcgaagc ggatttgctg cattaccgcc tgcgccaatg
     2041 cctgcgcctg aacaacgtcg aaattgaatt tttcgaggat ttcgttcaac aggcggattt
     2101 cgccaccgct aaattccgca ctgtgtttga ggatgaggcg ttgataatct tcgttgttga
     2161 ttttaggcaa taaatcggtt ttcagggtca tgattttatg tcgggggcgg aatggctgaa
     2221 gcgtgaatta tagcggatat ggcggcggct gttgcaaagc ttggattggg tatcgtcctc
     2281 aaaa
//
LOCUS       DAXHRI010000215         2281 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXHRI010000215 DAXHRI010000000
VERSION     DAXHRI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.215"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(26..1435)
                     /gene="dacB"
                     /locus_tag="ACFM75_002016"
     CDS             complement(26..1435)
                     /gene="dacB"
                     /locus_tag="ACFM75_002016"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC6256482.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDIADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1600..2166
                     /locus_tag="ACFM75_002017"
     CDS             1600..2166
                     /locus_tag="ACFM75_002017"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC6256483.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          518 a          555 c          661 g          547 t
ORIGIN      
        1 atttaatcca ctatattttc ctgtttcagg ctcggcgttc tttgcacatc agtttcgcat
       61 ccagccagcc gtcgccgccg gagatgatgt ttttggcaac gaagttgtcc aagtcgggca
      121 gcagggaaac ggcgcggccg ctgttgatga tgacgaccac cgccatcggt ttgtcgccca
      181 gccaataacc tgcaagggcg cggacattgt tgagcgtgcc ggtttttaag cgcaacagcc
      241 cgccgctttg tttgaagcgg ttgcgtaaag ttccgtctgt gccggcgatg ggcagcgtgt
      301 cgatgaaatc ttgtgcaaac gggctgaaat aagccgtttc caacatttgc gccatcattc
      361 tcgccgttac cctttctttt ctggacagac ctgaaccgtt ttccaaaacc aaatccgcaa
      421 tatcgatgcc cgacacggca agttcgcgcc ggacggcaga cgccgcctgt tcggaaacgg
      481 cgggcagttt gccgtcgccg ccgagtttga ggaagacgga acgcgcaatt agattgtccg
      541 aacgcttgtt catgtccgtc aaaatttcct tcatcggctt tgagtgtgca acggcaagcg
      601 tctgcgcgcc ttccggcgtg tcggatatgc cgatgccgtc tgaaatccgt ccgccgccga
      661 gcagccagtg gttggtaaaa ctttgccgga tcagttcgtc aagcgcgaac atccggacac
      721 cgacaggctt gcccaaacag ctctcgggaa tattgccgcg caatttcagc gtattgtccg
      781 aaaaagatgc acgcatcagt tttttgatcg aagggcaggc agcttgggag gcggtaattt
      841 tcaagttgtt ttgggcgaaa atatgcggca aaggcggatc ggtgaggatg tcggtactgt
      901 cggcggcatt gcgttcggcg cgcaccataa ccataccggc agacagcata gttggattgg
      961 ggggcgtcat aaacggcgaa ccgctgtcgg cttcgaaatc gtcggggctg ccgacttcgc
     1021 cccacaggct gtggtcgagc atcaggtgtc ccgtgatatt gcgtatgcct tgttcgcgca
     1081 actggcgttg gacggcaagc aggttttcct gattgaaaac ggggtcgccg ctgcccgccc
     1141 aatacaggtt tccgtcaagc gtgccgtcgt ttaccgtacc gttgctttta aactcggtcg
     1201 cccagcggta attgctgccg aaggttttga aggcggcaaa cgcggtaacg agtttcattg
     1261 tggaggcggg gttgacgggg acatccgagc ggtggtcaat gatgactttt ccgctgtcaa
     1321 gctcttggac atatacggcg atttcgtttt gcggaatgcg acctgtatcg agcgcgtgtg
     1381 cggcgaggga ggcgagaagc agcagcaggg aggccgctgt tttggggaaa ttcataggta
     1441 aatctgttac ataaaaaaag caattataag gcaaagccgg ataagtggga acgctgtggc
     1501 ggcgggccgg cttgtttgca ggggaaatca catatataat aatcgctatc attatgaaat
     1561 gattgagacg cacaaactta atattcagga ggaatgatta tggctaagaa aatcagtatt
     1621 ttggtgggca gcctgcgccg tgcttctttt gcgcgcaaag tggcattgaa tgcggcggag
     1681 atgttccccg aaggctggca ggcggaaatc gtcgaaatcg gacatctgcc gctttacaat
     1741 ttcgattatg acgaccctgc ggtggaagat gtgccgctgc ccgaaagcta cacggttttc
     1801 cgcgaaacga ttaaggcttc ggacggcatt ttgttcgtta cgtccgaaaa taaccgcacc
     1861 attcccgcct gtttgaaaaa tgcggtggac atcggctcga aaccgaatgc cgacgtggcg
     1921 tggaaaaaca aaccggccgg catcatcagc cattccgtcg gcaagatggg cggttacagt
     1981 tcgcaaaaaa acctgcgcct tgcgctgtct tatttcgata tgcccgtaac cggacagccg
     2041 gaagtgtttt tgggcaattc gccgacactg tttgatgaaa acggcaaatt gatcgactcg
     2101 gcaagggatt ttgttcagtc atatattaat cagtttgtcg gtttgattga aagaaatgcc
     2161 aaataaacaa acaagtcgaa aggccgcaaa accggttcgg gttttgcggc ttttttatat
     2221 gggctgggca gcgtcaaggt ttgccgtttg ccggataatg cgcggcaaat gaccggcgtg
     2281 t
//
LOCUS       DAXHRI010000216         2195 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXHRI010000216 DAXHRI010000000
VERSION     DAXHRI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.216"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..188
                     /locus_tag="ACFM75_002018"
     CDS             <1..188
                     /locus_tag="ACFM75_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256484.1"
                     /translation="RSRALIAAKIDQAEISDSEKSWLKKELGKIKDTALSTLTENAIN
                     AIPAATLITLLKKFVGL"
     gene            complement(185..691)
                     /locus_tag="ACFM75_002019"
     CDS             complement(185..691)
                     /locus_tag="ACFM75_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC6256485.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLTDAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPVFVEVLEPFG
                     AEVEKKFG"
     gene            complement(694..891)
                     /locus_tag="ACFM75_002020"
     CDS             complement(694..891)
                     /locus_tag="ACFM75_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256486.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(888..1154)
                     /locus_tag="ACFM75_002021"
     CDS             complement(888..1154)
                     /locus_tag="ACFM75_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256487.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAQADRKNIAASLERIENKLEKVNEKLDRKADKS"
     gene            complement(1166..1504)
                     /locus_tag="ACFM75_002022"
     CDS             complement(1166..1504)
                     /locus_tag="ACFM75_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256488.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYNTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(1501..1719)
                     /locus_tag="ACFM75_002023"
     CDS             complement(1501..1719)
                     /locus_tag="ACFM75_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256489.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1732..1902
                     /locus_tag="ACFM75_002024"
     CDS             1732..1902
                     /locus_tag="ACFM75_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC6256490.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(1947..>2195)
                     /locus_tag="ACFM75_002025"
     CDS             complement(1947..>2195)
                     /locus_tag="ACFM75_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256491.1"
                     /translation="ITACSSTTAPSATEIKVVEKAVLPTPPAALMVAPVRPNPPKDGK
                     TVTLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
BASE COUNT          533 a          611 c          484 g          567 t
ORIGIN      
        1 aaaggtctcg ggctttaatt gcggcaaaaa tagaccaagc ggagatttct gattcagaaa
       61 aaagctggtt gaaaaaagaa ttagggaaaa tcaaggacac tgcattaagt acgcttacgg
      121 aaaatgcaat taatgctatc cctgccgcca ccctcattac tcttttaaaa aaatttgttg
      181 gcctctaacc aaacttcttc tccacttccg ccccaaacgg ctccaatacc tccacaaaga
      241 caggcaaatg tttgggatgt ttttcttgca caaacgccat caagaactca atcaattcca
      301 aagccgtcgc cagttttgat gtttccggca tcacacgggc attggcggat acggttttcg
      361 taaacgcatc ggtcaggctg gccaacagct tggcgcggtc ggacggaggc aaatcttcgg
      421 tactcgaatc ctgcagcatc gtcatcgtgc tgttgtactg caccataaaa ccggccaaca
      481 tcgcgcggct caaatcctca ataccgccgc cggccaaagt gtaggcggcg cgcatcttat
      541 cccagtcgtc gcctttttcc ttatccgcac gtttccacgc acgcgcagtg gtctgcggga
      601 tttcgcacat caaggccgcc gtttccaaag tttgctcgcc gctcacatag agccggcgta
      661 acttttcacg gatttcttgc gggtgagcca taattacagt cccattttcg ctttaagcaa
      721 ttcccagccg accgtaatca cgccgccgcc cagtgcgccg aatgtaatgg ccgtgcgttt
      781 cgtgtcttgg cggatttgcg caatttccgc ctgcatttct ttctgatttt tcagcgtttg
      841 atcagtcttg ttttcaatac gcgccaaggc ttctaaaatc gggtcgctca tgatttgtct
      901 gctttcctgt ccagtttttc gtttactttt tccaacttgt tttcgatgcg ctccaaagac
      961 gctgcgatat ttttgcggtc ggcttgggca tcctgcttgg tgtggtacga gagcttgacc
     1021 gcgtgcagct cctctttcag gtcgtcgata cgcttgtccg cttctttcag acggcctgaa
     1081 ataccgttga cccaaaacca aaacgccgct gtcgcaatcg gccacagggt cctaaatcca
     1141 aattcaaaat ccatttaaaa cccctttaaa caggcacatc gccgaatacg atacggacgg
     1201 cgtaaccgtc agggcgattg ctggcaatct ccagtccatc accatcatta caaacacagt
     1261 aatacgcccc caaagtttgc caaaccgcac gtttaaaggt gttatagttc gtaattggat
     1321 attcaaggtg aaacgtcgtc tgaaaatcct tatccatgcg taccgcgtac tcaatgcctg
     1381 ccttatccaa cagatcggaa acatgaatga caaacggctc ttgttcgcgt gcgcggctta
     1441 agcccaattc caaatcggca tggcgcacag ccaactgacg ttcaactaat tcacggtagg
     1501 tcattctttg atacccatta aatattttgt ccgcttgtac aatttcttaa cccacgaaat
     1561 atttacaaat gtataaacct ttgctacaac ttcgccgtca tagtgcgcat tttctcgtgc
     1621 agcccgaaat ttcgccctgg cttcttcagg gttgtccgcc caaatgctca atgaccagga
     1681 cttgccgtta aagcggtaag aaaacgtgta ctcattcata ggagaaacct tatgtatttt
     1741 gaaatctata aagacgcaaa aggcgaatac cgttggcgtt tgaaggcagc caaccatgaa
     1801 atcatcgctc agggcgaagg ctacaccagc aagcaaaact gccagcacgc agtcgatttg
     1861 ctgaaaagca ctaccgccgc tacccctgta aaagaggtat aaaatccgct ttcaccctca
     1921 gcccgcgccc tacgcgggct tttttgtcag tttccgactt tgcgggagtg attgcccgcc
     1981 cagtcgcgcc aagcctgatt ttgattttcc aactccgcca catagccgcc aaactcggcg
     2041 gcgtgttcca acagcgttac tgtcttgccg tctttcggcg gattcgggcg taccggcgca
     2101 accatcaatg cggcaggcgg tgtcggcaag accgcctttt cgacaacctt aatttccgta
     2161 gccgagggcg cggttgtaga gctgcaggcc gtgat
//
LOCUS       DAXHRI010000217         2176 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXHRI010000217 DAXHRI010000000
VERSION     DAXHRI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.217"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="ACFM75_002026"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="ACFM75_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC6256492.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKHTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(1935..>2176)
                     /locus_tag="ACFM75_002027"
     CDS             complement(1935..>2176)
                     /locus_tag="ACFM75_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256493.1"
                     /translation="LPKLVRTQKAYSFKRHCSTCCREAEPDTETRRGDMPLEKWRQFL
                     DWVGKTPSETASRSRKARPNKKCRLNFFQTASSII"
BASE COUNT          547 a          630 c          572 g          427 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 cctcgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg atacgccggg acacgccgac ttcggcggcg aagtagagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caataaaatc gacaagccgt ctgcacgtcc gagctgggtt atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt acgcttcagg
      601 tttgagcggc tttgccaagc tggaagaaac cgacgagagc agcgatatgc gcccgctgtt
      661 cgacaccatc ttaaaacaca cgcccgcacc gagcggcagc gcggacgagc cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggcatcg gtcgtatctt
      781 gaacggacgc atcaaacccg gccaaaccgt tgccgtcatg aaccacgagc agcaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggcttg gaacgcgtac cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtatcgaa gacatcggca tcggcgtaac
      961 catcaccgac aaagacaacc ccaaaggcct gccgatgttg agcgtggacg aaccgacgct
     1021 gacgatggac tttatggtca acaccagccc gctggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagacacc gccgatgccg acgtgttccg cgtatccgga cgcggcgagc tgcacctgac
     1201 cattttgttg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccttat gaaaacctga ccgtggacgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgacggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aatttatgac cctgacgcgc ggggtcgggc tgatgagcca
     1501 cgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg
     1621 ccgtatgttc gtatcgccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 tcgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgtgc
     1741 cagcggtacc gacgaagccg ttcgcctgac cacgccgatt aagctgacgc tggaaggtgc
     1801 ggtcgagttt atcgacgatg atgagctggt agaaatcacg ccgcaatcca tccgcctgcg
     1861 caagcgttac ctgagcgaat tggaacgccg ccgccatttc aaaaagctag attgatgttt
     1921 actggattta atgtttaaat gatactcgat gccgtctgaa aaaagttcag acggcatttt
     1981 ttattcggac gggctttgcg gcttcttgaa gctgtttcag acggcgtttt tcctacccaa
     2041 tcaagaaact gccgccattt ttccagcggc atatcgcccc gtcgcgtttc ggtatcgggt
     2101 tcggcttccc ggcagcatgt cgaacaatgc cgtttgaagg aatacgcctt ttgagtcctt
     2161 acaagcttag gaagag
//
LOCUS       DAXHRI010000218         2146 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXHRI010000218 DAXHRI010000000
VERSION     DAXHRI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.218"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            32..465
                     /locus_tag="ACFM75_002028"
                     /pseudo
     CDS             32..465
                     /locus_tag="ACFM75_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            561..1184
                     /gene="rsmG"
                     /locus_tag="ACFM75_002029"
     CDS             561..1184
                     /gene="rsmG"
                     /locus_tag="ACFM75_002029"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC6256494.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRLDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1283..2056
                     /locus_tag="ACFM75_002030"
     CDS             1283..2056
                     /locus_tag="ACFM75_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC6256495.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVKSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
BASE COUNT          429 a          486 c          733 g          498 t
ORIGIN      
        1 atgcggtctg cttcggaaga gggggacggc gatgtttgtg aacgagaaag atccttatgc
       61 ggctctgttt gcgggactgg tgtttttgac gctgccgttt gcgttggcgg tgcatgatgc
      121 ctttgcgctt gcgttcggac gggcggggct gctggtgtcg gtgtcggacg gcggattcgg
      181 ctggcgtggc ggttgggacg gcactgtttg gtttgtgttc ggtgtgtttg cgtttttgaa
      241 tgtggttgtg tcggcgggcc tgacgaaact ggcgtacaaa aagatgatgc ggcggcattc
      301 gcgtggattt tcgagctgct gtttggcagt gccgccttgg gcgggctgcg tggggaggcg
      361 gtgttggaat atgcgtttgc cgtgtggctg gtgtcgatgc tgacactgcc caaacgcttg
      421 acgcgcgcgc cggtgcagcc ggtggtgttt cacaggaaaa aataggacgg aacgggggat
      481 gccgtctgaa acccgacacg cggtttcaga cggcatgttt ttccgttaac attacgcctg
      541 aatatggaca ggaagcagat atggaacgca aagaacgcct gcgtgcaggc attgccgcga
      601 tggggctgga tatttcggaa acggcacagg acaggctttt ggcctatgtg gatctgttga
      661 aaaagtggaa caaaacctac aatctgaccg ccctgcgcga cgaggaaaaa atgattgtcc
      721 atcatctttt ggacagcctg acgctgctgc cccatatcga gggtgtgcaa acgatgctgg
      781 atgtcggttc gggcggcggt cagcccggca ttccggcggc ggtgtgccgt ctggatgtgc
      841 agataaccct tttggatgcg aatacgaaga aaacggcttt tttacagcag gcggttatcg
      901 agttggggtt ggacaatgtg cgcgtggtat ccggacgcgt ggaggcggtt tcggacgtgc
      961 gtgccgatgt ggttaccagc cgtgcgtttg cagaactggc ggattttgtg tcgtggacgg
     1021 tgcatctgtt gaaagacggc ggctactggg cggcgatgaa gggcgtgtat ccgcaggaag
     1081 aaatcggccg cctgccgcag gatgtgtgcg ttgaaaaagt ccaaaggctc gacgtgccgg
     1141 gcttggatgc ggaacgccat atcgtcatcc tgagcaagcg ttgagcgcac ttcagacggc
     1201 atgaatacct tttttgtgcg gataaaggta aaattccgca ctgtttttct tttttcaaca
     1261 tcagacggga cacgggcggg acatgagtgc gaacatcctt gccatcgcca atcagaaggg
     1321 cggtgtgggc aaaacgacga cgacggtaaa tttggcggct tcgctggcat cgcgcggcaa
     1381 acgcgtgctg gtggtcgatt tggatccgca gggcaatgcg acgacgggca gcggcatcga
     1441 caaggcgagt ttgcagtccg gcgtttatca ggtcttattg ggcgatgcgg acgtgaaatc
     1501 ggcggcggta cgcagcaaag agggcggata cgctgtgttg ggtgcgaacc gcgcgctggc
     1561 cggtgcggaa atcgagctgg tgcaggaaat cgcccgggaa gtgcgtttga aaaacgcact
     1621 caaggcagtg gcggaagatt acgactttat cctgatagac tgcccgcctt cgctgacgct
     1681 gttgacgctt aacggcttgg tggcggcagg cggcgtgatt gtgccgatgt tgtgcgaata
     1741 ttacgcgctt gaggggattt ccgatttgat tgcgaccgtg cgtaaaatcc gtcaggcggt
     1801 caatcccgat ttggacatca cgggcatcgt gcgcacgatg tacgacagcc gcagcaggct
     1861 ggttgccgaa gtcagcgaac agttgcgcag ccatttcggg gatttgcttt ttgaaaccgt
     1921 catcccgcgc aatatccgcc ttgcagaagc accgagccac ggtatgccgg tgatggctta
     1981 cgacgcgcag gcaaagggta ccaaggcgta tcttgccttg gcggacgagc tgatggcgag
     2041 ggtgtcgggg aaataggtca atccaaatcg ggctgcccgt gcctttatgc tgtttggccg
     2101 ggtgcgttaa tatggcggat taaaataaaa atacttatat cgtcat
//
LOCUS       DAXHRI010000219         2145 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXHRI010000219 DAXHRI010000000
VERSION     DAXHRI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.219"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(102..1181)
                     /gene="aroB"
                     /locus_tag="ACFM75_002031"
     CDS             complement(102..1181)
                     /gene="aroB"
                     /locus_tag="ACFM75_002031"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC6256496.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLHTLQTALDAAGVSHFSIILPDGEAYKNWQTLNLIFDGLMQHRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGTLGDISFFEWLEQHMPELMALERAPLIQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKISAADTARLAALFEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
     gene            complement(1261..1773)
                     /locus_tag="ACFM75_002032"
     CDS             complement(1261..1773)
                     /locus_tag="ACFM75_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC6256497.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
BASE COUNT          433 a          522 c          677 g          513 t
ORIGIN      
        1 gaccgaaacc gaacggactg gattcccgct tttgcggaga aagatgatgg aaaatcatca
       61 tcgtgcgtca aacccgcgcg gcagcacatc ggcagaggaa atcagagata cggttgcagg
      121 gtgcggcgga ggatgtccgt gtcggtaatt tcggtgatgt tggcttcgcc cagccggttc
      181 agaccgataa agcgcatgat gccgccgctg acttttttat cgtggctcat atgttcgagc
      241 catttttcaa aggcaaacac gggcggtgcg gacggcagtc cggcggcttc aaacagggcg
      301 gcgagccgcg cggtatcagc ggcggagatt ttgcccagtt gttcggacaa acgcgccgcc
      361 aacacgcagc cggcggcgac ggcttcgccg tgcagccata cgccgtaacc catttccgct
      421 tcgacggcgt gtccgaaggt gtgtccgagg ttgagccatg cgcgtatgcc ctgttcggtt
      481 tcgtcttggg cgacgatgtc tgccttcatt tggcaacagc ggtacacggc ttggatgagg
      541 ggcgcgcgtt cgagtgccat cagttcgggc atatgctgtt ccagccattc aaaaaaacta
      601 atgtcgccga gcgtgccgta tttgatgact tccgccatac cggcggacag ttcgcgggcg
      661 ggcagggtgt gcagcgtgtc caagtcggca agcaccgcct gcggctggta gaacgcgccg
      721 atcatatttt tgccgagcgg gtggttgatg gcggttttgc cgccgacgga ggaatcgacc
      781 tgactcaaca gtgtggtcgg tatttggatg aacggagccc cacgctggta ggtggcagcg
      841 gcaaagccga ccatgtcgcc gataacgccg ccgcccagcg cgattaatgt ggttttgcgt
      901 tcggcgcggt gttgcatcag accgtcaaag atgaggttga gcgtctgcca gtttttgtac
      961 gcctcgccgt cgggcaggat gatgctgaaa tgggacacgc ctgccgcgtc caatgccgtc
     1021 tgaagcgtgt ggagataaag cggggcgacg gtttcgttgg tgatgatggc ggcgcgtttg
     1081 cccaaatgcg gtttgagcag gctgcctgcc tgcggcagca gcccgttgcc gataaagatg
     1141 gggtagctgt gggacggggt gtgtacggtc agtgttttca ttgttgttcc ttaaagtttg
     1201 aaccgccggc ccgccgggcg gggcgcggct tggttttctg ggcgcggcgc atatgccggt
     1261 ttatcgggat aagcgtttga gcagggtttg cacggtttcc cggcagtttg ccgattctac
     1321 ggtaaagtcg gcggtttggc ggtaaacggg gtcgcgtgcg gcgtagagtt cacgtaattt
     1381 cgccaaagga tcggcaactt gcagcaaagg acggctgttg tcgcagcgcg tgcgttcgag
     1441 cagggtttcg ggcggggcgt gcagatagac gaccgtgccg cttttgcgga taagggcgcg
     1501 gttttcttct tttaacaccg cgccgccgcc ggtggacagg acgatatggg gcaggataac
     1561 cagctttttg agtatggcgg tttcgcgcga acgaaatccc tgttcgcctt ccatttcaaa
     1621 tatggtgggg atgggaacgc cggcggctgc ggcgatttcg tgatcgctgt cgtaaaaacg
     1681 gtaatccagc cgctgcgcca tttgccggcc cagcgtggtt ttgcccgcgc ccatcagtcc
     1741 gatgaggatg agtttgccgt taaagttttt catcacaatt ccttaatgtt tgacaccccg
     1801 cctttcgggg cgacagggtt cgggcttgtc gggttacggc ggaattttat acgaaatcgg
     1861 cagggcggcg gtacgtttgg aaaaataacc cgaccatccc gaacctttct gattttaagg
     1921 aaaaataaaa gaaatcaggg aggtttttta tttcaggctg tgttttgaca atccgttgat
     1981 ttcacttatt tgtcaggaaa aggcaattat ctttgcttag gtaaacaatt atccaattga
     2041 atatattgaa gataatatgt ttatcaatac tatagtggat taacaaaaac cagtacagcg
     2101 ttgcctcgcc ttggctcaaa gagaacgatt ctctaaggtg ctgaa
//
LOCUS       DAXHRI010000220         2105 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXHRI010000220 DAXHRI010000000
VERSION     DAXHRI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.220"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..1945
                     /gene="pilC"
                     /locus_tag="ACFM75_002033"
     CDS             <1..1945
                     /gene="pilC"
                     /locus_tag="ACFM75_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6256498.1"
                     /translation="HSLATLTENDIKNREPNFTGRQTIIRLDGGVQQIKLGRNNDEVV
                     NFNGNNGNNATFGIVKDLGVDPDANEWKKVLLPWTVRAFNDDGRFNTVNKEENNGKPK
                     YSQRYRIRENGNNGKRNLGDIVNSPIVAVGEYLATSANDGMVHIFKQSGGDERNYSLK
                     LSYIPGTMPRKDIQSQDSTLAKELRAFAEKGYVGDRYGVDGGFVLRQVNNLNGQDRVF
                     MFGAMGLGGRGAYALDLTKADGSDPTAVSLFDVKHDNNGKNSNNSVQLGYTVGTPQIG
                     KTHNDKYAAFLASGYATKDINNGENKTALYVYDLENNGNLIKKIEVKDGKGGLSSPTL
                     VDKDLDGTVDIAYAGDRGGSMYRFDLSSQDPQQWSVRTIFSGNKPITSAPAISQLKDK
                     RVVIFGTGSDLSEDDVDNNDMQSIYGIFDNDTAAGNANANLSGLGGGLLEQKLTQEDR
                     TLFLTDYKRSDGSGSKGWVVNLQPGQRVTVKPTVVLRTAFVTIRKYKDNGCGAETAIL
                     GINTADGGKLTKKSARPIVPADNTAVAQYSGHKQTAKGKSIPIGCMEKNGGTVCPNGY
                     VYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPDGKRAGKNNRCFSQKGVRTLLMND
                     LDSLDITGPTCGMKRISWREVFF"
BASE COUNT          589 a          527 c          556 g          433 t
ORIGIN      
        1 ccattccctc gcaaccttga ctgagaatga tattaaaaat cgagagccga atttcacagg
       61 gcggcaaacc atcatccgat tggatggcgg cgtacagcag atcaaactgg gtagaaacaa
      121 tgatgaggtc gtcaatttta atggaaacaa cggcaacaac gcaactttcg gcattgttaa
      181 ggacttgggc gtcgatcctg atgccaatga gtggaaaaaa gtattgctgc cttggacggt
      241 tcgtgctttc aatgatgacg gtcgatttaa cacagtcaac aaagaagaaa acaacggcaa
      301 gccaaaatac agccaaagat accgcatccg cgaaaacggc aacaacggca agcgcaattt
      361 gggcgacatc gtcaacagcc ccatcgtggc ggtcggcgaa tatctagcta cttccgccaa
      421 cgacgggatg gtgcatatct tcaaacaaag cggcggggac gagcgcaact atagtctgaa
      481 gctcagttat atccccggca cgatgccgcg caaggatatt caaagccaag actccaccct
      541 tgccaaagag ctgcgcgcct ttgccgaaaa aggctatgtt ggcgaccgct acggcgtgga
      601 cggcggcttt gtcttacgcc aagttaacaa cttaaatggg caagaccgcg tgtttatgtt
      661 cggcgcgatg ggcctgggcg gcagaggcgc atacgccttg gatttgacca aagccgacgg
      721 cagtgacccg accgccgttt ccctgtttga tgtcaaacat gacaataatg gcaagaatag
      781 caataatagc gtgcaattag gctacaccgt cggcacgccg caaatcggca aaacccacaa
      841 cgacaaatac gccgccttcc tcgcctccgg ttatgcgact aaagacatta acaacggcga
      901 gaataaaacc gcgctgtatg tgtatgattt ggaaaacaac ggcaatttga ttaaaaaaat
      961 cgaagtgaag gacggcaagg gcgggctttc gtcccccacg ctggtggata aagatttgga
     1021 cggcacggtc gatatcgcct atgccggcga tcgcggcggc agtatgtacc gctttgattt
     1081 gagcagtcaa gatcctcaac aatggtctgt acgcaccatt ttcagcggca acaaaccgat
     1141 tacttccgcg cctgctattt cccaactgaa agacaaacgc gtggtcatct tcggcacggg
     1201 cagcgatttg agtgaggatg atgttgataa taatgatatg caatctattt acggtatttt
     1261 tgacaacgat acggcggcgg gtaatgcaaa tgcaaacctc agcggtttgg gaggcgggct
     1321 gctcgagcaa aagcttactc aggaggatag aaccttattc ctgaccgatt acaagcgatc
     1381 cgacggatcg ggcagtaagg gatgggtagt gaacttgcag cccggacagc gcgttaccgt
     1441 caaaccgacc gtggtattgc gtaccgcctt tgtaaccatc cgcaaatata aagacaacgg
     1501 ctgcggcgcg gaaaccgcca ttttgggcat caataccgcc gacggcggca agctgaccaa
     1561 gaaaagcgcg cgcccgattg tgccggccga caatacggct gtcgcgcaat attccggtca
     1621 taagcaaacc gccaaaggca aatccatccc tataggttgt atggaaaaaa acgggggaac
     1681 cgtctgcccg aacggatatg tttacgacaa accggttaat gtgcgttatc tggatgaaaa
     1741 gaaaacagac ggattttcaa caacggcaga cggcgatgcg ggcggcagcg gtatagaccc
     1801 cgacggcaag cgtgccggca aaaacaaccg ctgcttctcc caaaaagggg tgcgcaccct
     1861 gctgatgaac gatttggaca gcttggacat taccggcccg acgtgcggta tgaaacgaat
     1921 cagctggcgt gaagtcttct tctgatttgc acgcgaaaat gccgtccgaa aggttttcgg
     1981 acggcatttt ttgcgttttt cgggaggggc gggtttgtaa aaggcgggct atagggtagg
     2041 cttcatctcg ccaatctcac tgaatccatc aatttccaca attcaattaa ataccgtcaa
     2101 accga
//
LOCUS       DAXHRI010000221         2006 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXHRI010000221 DAXHRI010000000
VERSION     DAXHRI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.221"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..672
                     /locus_tag="ACFM75_002034"
     CDS             <1..672
                     /locus_tag="ACFM75_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFC6256499.1"
                     /translation="VTSSYDHVITGTKNFGKKASEQRPTITVQTLKQLGLSEQAAEYV
                     QFSIDLFSVGKSGASMPKAKPVSDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNS
                     NFSQHAQLEREINKLKSADEINFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEY
                     VEINGKAYIVRGNNRVFAAEYLGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRP
                     RYRSK"
     gene            675..1139
                     /locus_tag="ACFM75_002035"
     CDS             675..1139
                     /locus_tag="ACFM75_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256500.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            1364..1510
                     /locus_tag="ACFM75_002036"
     CDS             1364..1510
                     /locus_tag="ACFM75_002036"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256501.1"
                     /translation="MWYEANIGLINTMSRSNQAGTRLLYSNYGLLYITTDHYISATRF
                     VAWK"
     gene            1522..1809
                     /locus_tag="ACFM75_002037"
     CDS             1522..1809
                     /locus_tag="ACFM75_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFC6256502.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            complement(1826..>2006)
                     /locus_tag="ACFM75_002038"
     CDS             complement(1826..>2006)
                     /locus_tag="ACFM75_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256503.1"
                     /translation="IGGVKQRPSETVFLFLDGLLAQTLRTACVRSAHMLLVEFKFYMG
                     YGLLNWLWGVLSLCT"
BASE COUNT          730 a          301 c          401 g          574 t
ORIGIN      
        1 gtaacgtctt cctacgatca tgtcattact gggacgaaaa acttcggaaa aaaagccagc
       61 gagcaacgac cgacgattac ggtccagacc ttgaagcagt tggggttgtc ggaacaggct
      121 gcggaatatg ttcagttttc tatagatttg ttcagtgtgg gtaaatcagg agccagtatg
      181 cctaaggcta agcctgtgtc tgatgcaaaa ccgagatggg aggttgatag gaagcttaat
      241 aaattgacaa ctcgtgagca ggtggagaaa aatgttcagg aaataaggaa cggtaataaa
      301 aacagtaact ttagccaaca tgctcaacta gagagggaaa ttaataaact aaaatctgcc
      361 gatgaaatta attttgcaga tggaatggga aaatttaccg atagcatgaa tgacaaggct
      421 tttagtaggc ttgtgaaatc agttaaagag aatggcttca caaatccagt tgtggagtac
      481 gttgaaataa atggaaaagc atatatcgta agaggaaata atagggtttt tgctgcagaa
      541 taccttggca ggatacatga attaaaattt aaaaaagttg actttcctgt tcctaatact
      601 agttggaaaa atcctactga tgtcttgaat gaatcaggta atgttaagag acctcgttat
      661 aggagtaaat aaaaatggca atttggaatt atcggtacta ccttatccca tcagctgcta
      721 tcagaaataa atttaatgca aataaagata tcatacttga tgagtatcga tctaatggtt
      781 ttcagaattt taatgagaat aaaagttttg aaaattactt tatcgataat gatgttatat
      841 tattatcaat aataaatgaa gcaaaaaaac agcttaaatt gaaagaatct tgggataaag
      901 acgcaatcat gttttgtgat aattttggta atagtcttac cgtttggcca gatgatatag
      961 agtgcgaact tgatttaaga tttgattata ctaaatttat tcagaaaacc attgattggg
     1021 caataaaata taattgtcta cttgtaatag aaaaaacagg aaatgtagtt tcccctaata
     1081 taaataatct gatgtatgaa ataaaagcat atttggaaag caagccgtgg cccatatgaa
     1141 acctaaactc aacaagtagg atgtgtgcgg aacgcacgta tgcggttctc aaggtttgag
     1201 ctaagaggcc gtctgaaaac agaaaaactg tttcagacga cctttctttt aaccagttgc
     1261 cacagcaacc ggacaaaagc agcctacctc cacatccata taggcaatac aggggagata
     1321 ttttgtaaat tctacgaata ttttacctgc taaacagggt aggatatggt atgaagcgaa
     1381 cattggctta ataaacacta tgtcaagatc gaatcaggct ggtactagat tgttgtattc
     1441 caattatgga ttgctatata taacaactga tcattatatc tctgcaacac ggtttgtagc
     1501 ttggaaatag gagtataact tatgcaatta gagattatcg gtagtaaaat ttatacggaa
     1561 caagattttc ataatcaaat ttcaaaaata ttttctatac aagattatta tgggaacaat
     1621 cttgatgctt tatgggattt attaagcaca aatgtagaac gaccgattac tttggtatgg
     1681 aaagatgcta tgttctcaaa aaatcaatta gaaaatatat ttattgaaat cgtaaatgtt
     1741 ctagaaagag ttaagaaaca agatgaggat tatggattcg aagaaaaatt taattatatt
     1801 ttagagtaag taaaccctaa ttacattacg tacacaggct taaaactccc cagagccaat
     1861 taagcaagcc gtaacccata taaaacttaa actcaacaag tagcatgtgt gcggaacgta
     1921 cgcatgcggt tcttaaagtt tgagctaaga ggccgtctaa aaacagaaaa accgtttcag
     1981 acggtctttg tttaacgcca ccgatc
//
LOCUS       DAXHRI010000222         1949 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXHRI010000222 DAXHRI010000000
VERSION     DAXHRI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.222"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            248..1705
                     /locus_tag="ACFM75_002039"
     CDS             248..1705
                     /locus_tag="ACFM75_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HFC6256504.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          440 a          584 c          445 g          480 t
ORIGIN      
        1 aaaaccgccc tatcggataa gcccttaaca gaaaaggctt tacccgcgcc gtatcggaac
       61 acatcctcta aaatacaatc cgttgaattg aaaaaaatat aaaaacatcc gcccgcgaaa
      121 aacggcagcg cgtcgtttga caaagaatga aaatatcggt taaaaaccga ttttcataca
      181 aaaaacaccg ctgccgtccg catccgtttc agacggtatt gagagaaaat cttttaggag
      241 aacctttatg tcccggcatc ccgcccccac cggagaaaaa acattcttcg gccacccctt
      301 ccagctttcc accctcttcc atatcgaatt gtgggaacgt ttttcatttt acggaatgca
      361 gggcatcctg ctgatttacc tctactacac cgccgacaaa ggcggcttgg gcatagacaa
      421 aaccctcgcc ggcggcattg tcggcgcata cagcggcagc gtgtacctgt ccaccatttt
      481 gggggcgtgg tttgccgacc gagtatgggg tgcggaaaaa accctcttcc tctcgggcat
      541 cgtcgtgatg ctcggacaca tcgtccttgc cgccgccccg ggcctgtacg gccttttaat
      601 cgggctgata ttcatcgcat tgggcagcgg cggcgtgaaa tctacggcca gttctatggt
      661 gggcgcatta tacgaacagg acgaaatgcg cccgctgcgc gatgcgggat tttccatttt
      721 ctacatcgcc atcaatatcg gcggcttcct aggcccgctg ctgaccggcc tgctgcagga
      781 aaacatcggt ttccattatg gtttcggcgc ggcggcggtc ggtatggcat tcggcttgtg
      841 gcgttattcc ttgggacgta aaaacctgcc ccatcccatc gtcccccatc cgctttcaaa
      901 aggacagggc aaaactgcgg ccgccgtcgg cattgctctc atcgccgcac ttgcaaccgc
      961 catcaaaacc gggcttgtca acctcgacaa tttctccggc atcctcttat ctaccgtcat
     1021 ccttgccgtc attgcctatt ttgcccgcct gctgactaat ccccgcgtca gttccgacaa
     1081 caaacggcac atcatcgcct acatcccgct tttcctgacc atctgtatgt tttgggccgt
     1141 ctggtttcag atttacaccg tagcaaccgt ctatttcgac gaaaccgtca accgcaccat
     1201 cggttcgttt accgtgcccg tcgcctggaa agattccatg caaagcctgt gggtcatcct
     1261 gttttccgga ctgatggcgg caatgtggac aaaaatgggg cgcaaacagc ccaaaacccc
     1321 gctgaaattc gctatggcgg tatttgttac cggcgcgtcg tttttgggat tcgtcccctt
     1381 tatttccgcc ggcacgccga tgcctattgc ggttttcgca ctgatcgtcc tcgccatcac
     1441 gataggcgaa ctgatgattt ccccgattgc gctgtccatc tccaccaaaa tcgcaccgcc
     1501 tttattcaaa acccaaatgg tcgcccttaa tttccttgcc ttttcattag gcttcacttt
     1561 gggcggcgta ttgtttgaaa aaggctatca ggcgggcgac gaaatcggct tctaccggct
     1621 gctgttctac atcggcgcag ccacaggctt cctgctgctc ctgctcgtcc ccaaattgaa
     1681 caaaatgctc gaaggcacag actaagtccc gccccgatgc cgtctaaacc cttcagacgg
     1741 catttttccg cataatgaaa ccaaaccgtt tccacccgac aggacaggct cccccccaac
     1801 cggaaggcag cctgccgatt gtcatttaaa tatctgactg attccgtctg tttgcaaggg
     1861 aaatagccgt ttatttccgc ttgggcggaa accggttcta ttgagtaaaa ggcattttat
     1921 ccgactaaat tagtgctgca tcaacggaa
//
LOCUS       DAXHRI010000223         1942 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXHRI010000223 DAXHRI010000000
VERSION     DAXHRI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.223"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..597
                     /locus_tag="ACFM75_002040"
     CDS             <1..597
                     /locus_tag="ACFM75_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6256505.1"
                     /translation="YGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGWV
                     LEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPEY
                     EAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDNYSTSVGSCINGFTV
                     QYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            614..>1942
                     /locus_tag="ACFM75_002041"
     CDS             614..>1942
                     /locus_tag="ACFM75_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein frpC"
                     /protein_id="HFC6256506.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFRQYARLINENNGR
                     LPNTSEIERSYYKAVTDNGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSEWDNRKQLISALDKGFDGSFKEKPFTFLQSVIMDVTKLSVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGALFDHRNQGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQSLRVW
                     QDLNQDGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKT"
BASE COUNT          615 a          363 c          447 g          517 t
ORIGIN      
        1 tatggctatc ttgcacggta tacgcaagac aatgccacaa aatggctttc cgacacgccc
       61 gggcaggatg cttactccat taatttgata gagattagcg tctattacaa aaaaaccgac
      121 caaggctggg ttcttgagcc atacaaccag caaaacaaag cacactttat ccaatttcta
      181 cgcgatggtt tggatagcgt ggacgatatt gttatccgaa aagatgcgtg tagtttaagt
      241 acgactatgg gagaaagatt gcttacttac ggggttaaaa aaatgccatc tgcctatcct
      301 gaatatgaag cttatgaaga taaaagacat attcctgaaa atccatattt tcatgaattt
      361 tactatatta aaaaaggaga aaatccggcg attattactc attggaataa tcgagtaaac
      421 caggctgaag aagataatta tagcactagc gtaggttcct gtattaacgg tttcacggta
      481 cagtattacc cgtttattcg ggaaaagcag cagctcacac agcaggagtt ggtaggttat
      541 caccaacaag tagagcaatt ggtacagagt tttgtaaaca attcaagtaa aaaataattt
      601 aaaggatctt attatgaatg agggtgaagt tgttttaaca ccagaacaaa tccaaacctt
      661 gcgtggttat gctttccgtg gcgataccta tggcggttgg cgttatttgg ctaatttggg
      721 tgaccgttat gcggatgatg ctgctgcaat tgtcggtaag gatacaaact taaatggttt
      781 gaatttatgg atgaaaaaag gggtggaaaa cctatgggat gatacggtcg gtaaaaagac
      841 ccgtttagag aaatttgatc gggttgcact gcaacatttc aggcaatatg cgcgtctaat
      901 taatgaaaat aatggtagat tacctaacac tagtgaaatt gagagaagtt actataaagc
      961 cgttaccgat aatggcgttt cttccagtgc agctattgat ttagttatta atcgttcact
     1021 tccggatatg gcggatggtt attgggcatt aggtttgggg atagaagccg aacgtatcca
     1081 caatgagcaa gcagtaaata atccgaacgg tagcgaatgg gataatagaa agcagttaat
     1141 atctgcttta gataaaggat ttgatggatc ttttaaagag aagcctttta cttttttaca
     1201 atctgtgata atggatgtaa caaagttaag tgttgaatat acaatagatg gttggcaaaa
     1261 aattggaggt tggggtaatg ggataatcaa tgatttatat aaaagtgttg caaaaagaga
     1321 gtggactgga atatttgaga tcgttaataa taacatcaag caatttagag atctgttccc
     1381 aaatccggaa ggctggatcg atgatggtca ccaatgtttc gctccttggg ttaaagaaac
     1441 taaaaaacgc aatggcaaat atcatgtcta cgaccccctc gccctagatt tggacggaga
     1501 cggcatagaa accgttgcca ccaaaggctt tgcaggtgca ttgttcgacc accgcaatca
     1561 aggcatccgc accgccaccg gttgggtttc tgccgatgac ggtttactcg tccgcgattt
     1621 gaacggcaac ggcatcatcg acaacggcgc ggaactcttc ggcgacaaca ccaaactggc
     1681 agacggttct tttgccaaac acggctatgc agctttggcc gaattggatt caaacggcga
     1741 caacatcatc aacgcggcag acgccgcatt ccaatccctg cgtgtatggc aggatctcaa
     1801 ccaggacggc atttcccaag ctaatgaatt gcgtaccctt gaagaattgg gtatccaatc
     1861 tttggatctc gcctataaag atgtaaataa aaatctcggc aatggtaaca ctttggctca
     1921 gcaaggcagc tacaccaaaa ca
//
LOCUS       DAXHRI010000224         1935 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXHRI010000224 DAXHRI010000000
VERSION     DAXHRI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.224"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            1..1935
                     /locus_tag="ACFM75_002042"
                     /pseudo
     CDS             1..1935
                     /locus_tag="ACFM75_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201115.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
BASE COUNT          607 a          416 c          470 g          442 t
ORIGIN      
        1 ttcatgcagg taaggatctg gatttgaatg ccgataaaga cttatcaaca caaagtatca
       61 gcctgagggc agacaacacc gccctcatca gcagcaacgg taacaccttg actgcagaaa
      121 agaatctgga tattcaggca ggtagcttga gtgtgcgtca aagtaatctg caatccagtg
      181 gcggtaatgt gcagatgagc gcgactaaag gcaacatcag cttgaatcag agttggataa
      241 atgccagtca gaatattgat acggcagcac ttcaaggcaa tattatttca gacggtctga
      301 ctgctgttgc cgaagttgga cgcgtatctc ttcttgccaa cggcaatgtt gatttcaacg
      361 gtctaaatac cttgattgcg gaaggagaca ttaatgctgg ttcagtcggt aaaggccgtc
      421 tgaaaatgga caataccgat atttatgctt ctgcaggcga tgtgaaactg gttgccggag
      481 gtcaattaga tttaggcaac ggcaccgtta acggcggtca tatcagctta gacagcaata
      541 aaggcagcat ggtggtgcag gatgtacacc tgaatgcccg cgcatcactg aaagtggatg
      601 cggatcaaac tttaaccatt aataacagca agctcaattc cggtcacaat acctagatta
      661 atacaaatca tggtcatatg acgcttaatc agcttgatgc tcattcacgt cgtcatatga
      721 gtatcagtgc acagggtaaa ggcaaaggaa aagacagcgg tcaaatttta caaaacgacc
      781 agcaaaacag taaaagtact ttggcggcag atggtgtatt gtcattgaac agcagcgcat
      841 tacaggtttt ggacaatact accctgcgcg gtggcgcgat aaacatcaaa gctggcggag
      901 gcatcatcaa acggagccat atcgattggg aaacccaaga tacggcaact atgcgttcag
      961 cggaactgaa accactgtcc ggtatgatgt cgatagaatc gggcggtgat aaccctttaa
     1021 ccgttgaacc cggtaaccgt atcgtttctg caggtgattt ggccgtgaac cacaacggca
     1081 cattccaaat cagtgccaga gcaggaaata acggcaatcc gagtgcgcaa acagccagcg
     1141 tttcagctaa aggcaatatc gggattgtgg cgggagaggt ggatattgat gcagcaaata
     1201 ttgccgcagg gaaagatttg gctttagtgg caactaaagg gaatatttca ctaaacagta
     1261 ttaggaatac gtttagtaac tatcaattga aaacagataa acacaatatc acacagcaac
     1321 ttactgatgt agagcaggag cttagtaagt taacgagcga tcctaaatat cgtaaagcac
     1381 aggatcttcc acaaatgttg aggcgtaaat ataagaggcg tgacaaagta ttcggtgata
     1441 gtgaggccag attacgtggt ttacgagcaa agataaatgc tgctgatgag gcttgggcag
     1501 aactccaatc cccagtaaaa gcgttacttg aacgtaaaca attgttacaa caagcccttt
     1561 taacagtttc ccaaccgggt agtggtcacg aaaaccaagg tagcacccta gcggtcaaaa
     1621 tatcaaactg ctcgctgcag gtggtatccg tatccagggc tccaaagttg ctgccaccca
     1681 acaggcaaat atccaagctg caggattttt acctgcacca gccgcagagg aactgcaaga
     1741 aggtcgactt caatcagcca ttgacatcag cggtgtattt gatacttttg aatatggtca
     1801 gcaaggcagc gataaatacg gctatgccat tttcagcagg ccatctgaaa tttcaggaaa
     1861 aacaggtgtt accctctctg caccaaatgc caatgagaac agccgcatca gtctgagtgc
     1921 ggcaaacatc gaagc
//
LOCUS       DAXHRI010000225         1897 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXHRI010000225 DAXHRI010000000
VERSION     DAXHRI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.225"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            297..1463
                     /locus_tag="ACFM75_002043"
     CDS             297..1463
                     /locus_tag="ACFM75_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFC6256507.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEQP
                     SKAQGQTNNQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGDELKAVWQLEQDVSVAGG
                     GATRWGNRESFVGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDD
                     MPVSVRYDSPDFSGFSGSVQFVPIQNSKSAYKPAYVDEKKMVHAAVVGKPGSDVYYAG
                     LNYKNGGFAGSYAFKYARHANVGRDAFELFLLGSTSDEAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENGDKAKTKNSTTEIAATASYRFGNAVPRISYAHGFDLIERGK
                     KGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          495 a          426 c          488 g          488 t
ORIGIN      
        1 gtgccgcgtg tgttttttta tggcgtttta aaaagccggg actgcatcca ggcagtagtg
       61 catcggctcg cacgaggtct gcgcttgaat tgtgttgtag aaacacaacg ttttttgaaa
      121 aaataagcta ttgttttata tcaaaatata atcattttta aaataaaggt tgcggcattt
      181 atcagatatt tgttctgaaa aatggttttt tgcggggggg ggggtataat tgaagacgta
      241 tcgggtgttt gcccgatgtt tttaggtttt tatcaaattt acaaaaggaa gccgatatgc
      301 gaaaaaaact taccgccctc gtattgtccg cactgccgct tgcggccgtt gccgatgtca
      361 gcctgtacgg cgaaatcaaa gccggcgtgg aaggcaggaa catccagctg caattgaccg
      421 agcagccctc aaaggctcaa ggtcaaacga acaatcaggt aaaagttact aaggcaaaaa
      481 gccgcatcag gacgaaaatc agcgatttcg gctcgtttat cggctttaag gggagtgagg
      541 atttgggcga cgagctgaag gctgtttggc agcttgagca agacgtatcc gttgccggcg
      601 gcggcgcgac ccgttggggc aatagggaat cctttgtcgg cttggcaggc gaattcggca
      661 cgctgcgcgc cggccgcgtt gcgaatcagt ttgacgatgc cagccaagcc attgatcctt
      721 gggacagcaa caatgatgtg gcttcgcaat tgggtatttt caaacgccac gacgatatgc
      781 cggtttccgt acgctacgac tctccggact tttccggttt cagcggcagc gtccaattcg
      841 ttccgatcca aaacagcaag tccgcctata agccggctta tgtggatgag aagaagatgg
      901 ttcatgcggc tgttgtcggc aagcccggat cggatgtgta ttatgccggt ctgaattaca
      961 aaaatggcgg atttgccggg agctatgcct ttaaatatgc gagacacgcc aatgtcggac
     1021 gtgatgcttt tgagttgttc ttgctcggca gcacgagtga tgaagccaaa ggtaccgatc
     1081 ccttgaaaaa ccatcaggta caccgcctga cgggcggcta tgaggaaggc ggcttgaatc
     1141 tcgccttggc ggcccagttg gatttgtctg aaaatggcga caaagccaaa accaaaaaca
     1201 gtacgaccga aattgccgcg actgcttcct accgcttcgg taatgcagtt ccacgcatca
     1261 gctatgccca tggtttcgac ttgatcgaac gcggtaaaaa aggcgaaaat accagctacg
     1321 atcaaatcat cgccggcgtt gattatgatt tttccaaacg cacttccgcc atcgtgtctg
     1381 gcgcttggct gaaacgcaat accggcatcg gcaactacac tcaaattaat gccgcctccg
     1441 tcggtttgcg ccacaaattc taaatatcgg ggcggtaaag cggatagctt tgtttttgac
     1501 ggcttgcctt cattctttga ttgcaatctg actgccaatc tgcttcagcc ccaaacaaaa
     1561 atccggatac ggaagaaaaa cggcaataaa gacagcaaat accgtctgaa agattttcag
     1621 acggtatttc gcattttcgg cttggtttgc acatattgtg ggggactgtt agtttttgca
     1681 caaggaacaa atagagtaaa aaaacgctga aatcttcgga agacgtggat ttcggcgttt
     1741 tttgcatccg gaaaagttac gccagctttt tcacgaaacc gcgccggaat gcgcggtttt
     1801 ctgtttaaag ctgacgagat tagggaattt ttaaaactgt tttaagaggt ttttaaaatg
     1861 gatttaatca atactccggc cataccactc aacctgt
//
LOCUS       DAXHRI010000226         1853 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXHRI010000226 DAXHRI010000000
VERSION     DAXHRI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.226"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            208..1680
                     /gene="pyk"
                     /locus_tag="ACFM75_002044"
     CDS             208..1680
                     /gene="pyk"
                     /locus_tag="ACFM75_002044"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC6256508.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRTGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          472 a          537 c          508 g          336 t
ORIGIN      
        1 aaataaattt agcctaagtc ataaaaccgt gtaattttgc gccaaaaaag gcggtttgca
       61 ggtaaaatat caaactatta atttaaatca tatttttaca gaatattccg ccgcgttcat
      121 caaatggaca tcaaaccggt tccaaatttc tgtaattttg taacaaaata ccgcaaaaca
      181 cccgattgag accaaaagga ctttcatatg aaccagacaa gccgcgatct gacccgcatc
      241 agccacaaca ctaaaatcgt cgccaccctt gggccgggca gcaacaacgt cgaactgttg
      301 gaagacatga tccgcgtcgg cggtctgaac gtcgtccgct tcaacttcag ccacggcacg
      361 cccgaattcc atcaggaaaa cgcccgcatc gtgcgcgagg cggcaaaacg caccggacag
      421 gaaatcgcca tcattgccga cctgcagggc ccgaaaatcc gcgtgggcaa aatcgccggc
      481 ggcggcatcg aattgaacaa aggcgaaacg ctggtactgg atgccgcgct cgaaggcgaa
      541 ggcacgcgcg aggcggtcgg tttggactac cgcgacctgc ccgacgacgt tgccgcaggc
      601 gacgtattgt ggctggacga cggcctgctg accctgaccg tggaatccgt cgaaggcagc
      661 aggattatca caagggtgga aaacagccat gtcctgaaaa gcaacaaagg catcaacaaa
      721 cgcggcggcg ggctgtccgc aggcgcgttg accgaaaaag acttccgcga cctgaaaacc
      781 gcgattgcca tcggttgcga ctacctcgcc atcagctttg tgaaatccgc cgaagatttg
      841 catatcgcgc gcgccaaagt cgaagaagaa atgaagggca gcactgccgt gcgccccggt
      901 ttggtttcca aaatcgaacg cgtggaagcg attgaaaact tggacgaaat catccttgcc
      961 ggcgacggca ttatggttgc gcgcggcgac ttggcggtcg aagtcggaca cgccgccgtc
     1021 cccgccctgc aaaaacggat gatccgccgc gcccgcgagt tgcgccgctt cagcattacg
     1081 gcgacgcaaa tgatggaatc gatgattacc aaccccgtgc cgacccgcgc ggaagtcagc
     1141 gatgtggcaa acgcggtatt ggacggtacc gatgcggtga tgtgttccgc cgaaaccgcc
     1201 gtcggcgcgt atccgtttga aaccgtcagc caaatggcaa ttatctgcgc ggctgcggaa
     1261 aaagagcagg attcgctcaa cggcgttgcc gaacaggcgg agtatcccga agcggtcagc
     1321 accaacctgg cggttgccgg cggcgcggtc agcgtggcgc gcgcggttca cgccaaagcc
     1381 atcgtcgccc tgaccgaaag cggctcgacc gcctttgaaa tcagccgcca caacatcacc
     1441 ctgccgattt tcgcgctgac cccgagcgtt tccgcccaac gccgtatggc gatgtaccgg
     1501 ggcgtgcgcc cgctgatttt ggcaaccagt accgaccacg acacggcgtt gaacgaagtc
     1561 gaaacgatgc tggtggaaca caacatcctg cattccggcg accagtacat catcaccagc
     1621 ggttcgcaaa tgcgcgaatc cggttcgacc aatacgctgg aagtgctgcg cgtcaaataa
     1681 tccgccctga gtggaaaatg ccgtctgaag tcgatgcccg aggcttcaga cggcattttt
     1741 tgcggcggcg cggcggttcg ggcaaactct gaaaaaattg tgcaaatccg gcaacatcgg
     1801 ataaaatcga gtacctatac taaagcgaaa caaggcattt ccgactgcct ttt
//
LOCUS       DAXHRI010000227         1831 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXHRI010000227 DAXHRI010000000
VERSION     DAXHRI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.227"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..921
                     /locus_tag="ACFM75_002045"
     CDS             <1..921
                     /locus_tag="ACFM75_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6256509.1"
                     /translation="NTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGA
                     IGAAVGEILGETLLDGRDPGSLNVKGRAKIIAKAKLAAGAVAALSKGDVSTAANAAAV
                     AVENNSLNDIQDRLLSGNYALCMSAGGAESFCESYRPLGLPHFVSVSGEMKLPNKFGN
                     RMVNGKLIINTRNGNVYFSVGKIWSTVKSTKSNISGVSVGWVLNVSPNDYLKEASMND
                     FRNSNQNKAYAEMISQTLVGESVGGSLCLTRACFSVSSTISKSKSPFKDSKIIGEIGL
                     GSGVAAGVEKTIYIGNIKDIDKFISANIKK"
     gene            930..1352
                     /locus_tag="ACFM75_002046"
     CDS             930..1352
                     /locus_tag="ACFM75_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256510.1"
                     /translation="MKYMISFLKKTFELMSWVLVILIIGTFYDYYQIRQYAELEKKSI
                     SNILLYAQKEKFRLESKDKYMRGGYTKYKFIFSEYSNTTFLNFINDLKKDNYLPLDGY
                     GHGFLCRKGESISINIYPEINKFILVWGYPENLCADSN"
     gene            1389..1790
                     /locus_tag="ACFM75_002047"
     CDS             1389..1790
                     /locus_tag="ACFM75_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6256511.1"
                     /translation="MRKLNNHDVHKRYQDRLEEDVEFTINYELPLSCLWSTIKDFSSD
                     FEEKTEAFFILFKELLRRGHLKLQRDGQIIGHTPEEWEQIFREVWPEYEIEPNPLPGY
                     APFDIGMWLTVEAPAYAVWIDPEDGSEYWAG"
BASE COUNT          619 a          274 c          421 g          517 t
ORIGIN      
        1 aatactgcgc atggagaggc agcaagtaaa atcaaacagt tggatcagca ctacattgcc
       61 cataagattg cccatgccat agcgggctgt gcggcagcgg cggcgaataa gggcaagtgt
      121 caagatggtg cgatcggtgc ggcggtcggt gaaatccttg gcgaaaccct actggacggc
      181 agagaccctg gcagcctgaa tgtgaagggc agggcaaaaa tcattgctaa ggcgaagctg
      241 gcagcagggg cggttgcggc gttgagtaag ggggatgtga gtacggcggc gaatgcggct
      301 gctgtggcgg tagagaataa ttctttaaat gatatacagg atcgtttgtt gagtggaaat
      361 tatgctttat gtatgagtgc aggaggagca gaaagctttt gtgagtctta tcgaccactg
      421 ggcttgccac actttgtaag tgtttcagga gaaatgaaat tacctaataa attcgggaat
      481 cgtatggtta atggaaaatt aattattaac actagaaatg gcaatgtata tttctctgta
      541 ggtaaaatat ggagtactgt aaaatcaaca aaatcaaata taagtggggt atctgtcggt
      601 tgggttttaa atgtttcccc taatgattat ttaaaagaag catctatgaa tgatttcaga
      661 aatagtaatc aaaataaagc ctatgcagaa atgatttccc agactttggt aggtgagagt
      721 gttggtggta gtctttgtct gacaagagcc tgcttttcgg taagttcaac aatatctaaa
      781 tctaaatctc cttttaaaga ttcaaaaatt attggggaaa tcggtttggg aagtggtgtt
      841 gctgcaggag tagaaaaaac aatatacata ggtaacataa aagatattga taaatttatt
      901 agtgcaaaca taaaaaaata ggagttagta tgaaatatat gattagtttt ctaaaaaaaa
      961 catttgaatt aatgagttgg gtgttagtca tactaataat tgggacattt tatgactatt
     1021 atcaaataag gcaatatgct gaattagaaa agaaatctat atcaaatatc ttgctatatg
     1081 cccaaaaaga aaaatttcgc ttagagagta aagataaata catgcgagga ggatatacaa
     1141 aatataaatt tattttttca gaatatagta atactacttt tttaaatttc ataaatgacc
     1201 tgaaaaaaga taattattta ccacttgacg gctatggaca tggttttcta tgtagaaaag
     1261 gagagtctat atcaatcaat atataccctg aaattaataa atttatttta gtatggggat
     1321 accctgaaaa tctttgcgct gattctaatt aagtaagcaa gaataggtta tagggaaata
     1381 aaattcaaat gagaaaattg aataatcacg atgttcataa acggtatcaa gatcgtttag
     1441 aagaggatgt agagttcact atcaactatg agcttccttt gagttgtttg tggtcaacca
     1501 tcaaagactt ttccagcgat tttgaggaaa aaactgaagc gttctttatt cttttcaaag
     1561 agctgctgcg cagaggtcat ctgaaactgc aacgcgacgg gcaaattatc gggcatacgc
     1621 ccgaagaatg ggaacaaata tttagggaag tatggcctga atatgaaatc gaacccaatc
     1681 cacttcccgg ctatgcccca tttgatattg gaatgtggct tacggtcgag gctcctgcct
     1741 acgccgtatg gatagatccc gaagacggta gcgaatactg ggcgggataa aataccaatg
     1801 tttggaataa atcccgtctg aaaaacagct t
//
LOCUS       DAXHRI010000228         1831 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXHRI010000228 DAXHRI010000000
VERSION     DAXHRI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.228"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            95..382
                     /locus_tag="ACFM75_002048"
     CDS             95..382
                     /locus_tag="ACFM75_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256512.1"
                     /translation="MDDLIAAFLGYLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITEMMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
     gene            653..877
                     /locus_tag="ACFM75_002049"
     CDS             653..877
                     /locus_tag="ACFM75_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256513.1"
                     /translation="MMKNKYILTKAAISITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            965..1438
                     /locus_tag="ACFM75_002050"
     CDS             965..1438
                     /locus_tag="ACFM75_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256514.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
BASE COUNT          572 a          283 c          383 g          593 t
ORIGIN      
        1 gaggctgaag ggtaagagac gcttatatca tagatatagt ggcaaaacag gtaatgaatt
       61 aaagacatta cctgttttta cagaaatttt taaaatggat gatttgattg cagctttttt
      121 aggctattta tcaggtaaat atagcgaagg gaaaataagc agattagaaa tatctctaac
      181 ggcaggaagc attttctttg cagtattctt tattcatgaa ctgataattt ttattgttga
      241 tggaaataag gaaaatcttt ggaattttat tactgagatg atgaaagatt tatttttatt
      301 ttcatgttct gtttgtttat tattctactt cttattattt attgcggaaa ctattgggaa
      361 aacaatcaat aaaattaaat aagatattta agccgctaaa aaccatttaa gtattttatc
      421 ttttctatgc ggcatttatc ttggaattga aatactgcaa tcgggaggct aaggcttccg
      481 ttgtaacgtt taccatatat ttcattattt taatttcatc aaaaaaagaa attcaagatt
      541 atacatactg ccaatagttt cttgatcgtt ttgtatattt atctgatata tcaacatctc
      601 tctatcttat tccgaatttt attgttgaaa cttgcataaa aaaccataca agatgatgaa
      661 aaacaaatat attttaacaa aagcagcaat cagcataacc gccatatcta tattttatct
      721 tctaactcaa gaaagcagcg gcaaaactga agaaccatca tatttcttga tgtttatgtt
      781 tctcaacttg ctttggtttg aagaaaataa aacagttatg gctgccgtta cagcaatgat
      841 tgccgcccac tttatatttg tcgcactatc cgattaataa gcaatcaaaa aggctgcttg
      901 gaaatttcag gcagcctttc aggaggattt tgatggaaca ttatctcctc cctaagaatt
      961 tgttatgaaa aaacaaatca ctgaagccac gatgatgctg tctataatcg cccccgcaat
     1021 ggcaaacggc ttggacaatc aggcatttga agaccaagtg ttccacacgc gggcagatgc
     1081 gccgatgcag ttggcggagc tttctcaaaa ggagatgaag gagactgagg gggcgtttct
     1141 tcctattgtg gcaggtgctt tgatgggagg ggcaattagt gcttgggcta atcacggcat
     1201 ttctaaaatt aaaacagggc aatttgcttc aacaagaagt accttggctg caaccgctgg
     1261 tggtatgata ggaggcggat atagcagtgc gatgttgcgc ggtgcaggga ttactactag
     1321 tgtttttgcc cgttctgctt ggaaaggggg aaatgctatt cctaatgcag ttattcgtgc
     1381 aaatggtgca gcattaaatc agtcttctag cgccgcaatg ggaaaacata gaagataaac
     1441 gaaatggatg atctaatact atatttttta tcaggtatat tcggaaatca agttgctgaa
     1501 tatattataa aaaacaaccg tgagataaaa gttcccttta ttgtgcttta cgcaatattt
     1561 tttacgttga tttatactgt ggctttgttg tttctcagtc tgatctattg ggtcaatggt
     1621 gcggagattg catggaaggg gataggtatt ttcagtatgt cggtcagttt ttgtatagtc
     1681 ttctgtcttt atttgattga caaggcagga agatgtaagg ataagaaaca atagggatca
     1741 gatgcggcgg caaacgtaga ttgggtctag gctcaacctg cgtttgctgc atctttggaa
     1801 gaaatagtgg attcgaaaaa ataggaagaa t
//
LOCUS       DAXHRI010000229         1781 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXHRI010000229 DAXHRI010000000
VERSION     DAXHRI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.229"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(416..1411)
                     /gene="porB"
                     /locus_tag="ACFM75_002051"
     CDS             complement(416..1411)
                     /gene="porB"
                     /locus_tag="ACFM75_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC6256515.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVEHNGGQV
                     VSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGKYNSESYHAGFNYKNGGFFVQYGGAYKRHVRVDEKMNIEKYQIHRLVS
                     GYDNDALHASVAVQQQDAKLVEDNYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSF
                     DDADLSNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVSTAGGVGLRHKF"
BASE COUNT          457 a          405 c          440 g          479 t
ORIGIN      
        1 tcggaaatgg ctgaaactca aaaaactaga ttcccacttt cgtgggaatg acggcagagc
       61 ggattctgtt gctcccgata aatgccgcaa cctcaaatcc cgtcattccc gcgcaggcgg
      121 gaatctaggt ctgtcggtgc ggaaacttat cggataaaac ggtttcttga gattttacgt
      181 tctggattcc cactttcgtg ggaatgacgg gattaaagtt tcaaaattta ttctaaatag
      241 ctgaaactca acgcactaga ttcccgcctg cgcgggaatg acggcatatt ttgacattga
      301 ataaaaaaga ctaaaaacag aaaaaagcca aacagaaaaa agccaaacag aaaaaagcca
      361 aacagaaaaa agcctgtctg gcgacaggct ttttgttgat accaatcttt gcagattaga
      421 atttgtggcg cagaccgaca ccgccggcag tcgatacgaa tttgttttcg cctttacctt
      481 cttgcaacca accggcagaa accaaggcag aagtgcgttt ggagaagtcg tattccgcac
      541 cgacaaccac ttggtcgtaa tcgttgctta agtctgcatc atcaaacgag cctttgaagc
      601 cgtgggcgta agaaacgcgg ggcgttacgt tgccgaagcg gtatgccaag gtagcggcaa
      661 cttcggtttg agagttgtgc gaataattgt cttcaaccaa tttggcatct tgttgctgta
      721 cggctacgga agcgtgcagg gcatcattgt cgtaaccgct gaccaaacgg tgaatctggt
      781 atttctcaat attcatcttc tcatccaccc gcacatgtct tttataggca ccgccatatt
      841 gcacgaagaa gccgccgttt ttgtagttga agccggcgtg gtaagattcg ctgttatatt
      901 tgcctgcatt gtcgttaagc gcgtattgta cgctgccgct gaggccggca aattcgggag
      961 aatcgtagcg tacggaaatg aggcgtgctt cgggttcggc aattttgttt acacccaaat
     1021 agtcgctttt gctatcccaa ggattgatgt cgccggtgtc tttcaggacg ctgttcaaac
     1081 ggccgacgcg caatttaccg aagccgcctt tcaaaccgat gaaggattgg cggttgcccc
     1141 aaccggagtc agtaccggcg atagatgcct tttgctcaac ctgccaaatg gctttcagac
     1201 cgttaccgag gtcttcttgg cctttgaagc cgatttttga acccaaatca acgatgccgg
     1261 taccggtttc aacgctaacc acctgacctc cattgtgctc tacagagcgg gaagtttcta
     1321 cgccggcttt gatggtgccg tacagggtaa cgtcagccat tgctgcaaca ggaagggctg
     1381 ccaaagtcag ggcaatcagg gattttttca ttgctgtatt ccttttttgg ttaagaaatt
     1441 taagcaggcc gggctttcca agccgcttag ctttgcattt accgccgacg ttttcggcga
     1501 ttccatgtct gaaactatag atgttcctgt ctgaaaaaac aaacttctgc ctgtttttgt
     1561 gtggaattcg atgtgtattt tgaagggcgg atagagatat tgtgggtatt ttttgtttta
     1621 taacatatgg ttatttaaat tttttaagat ttgcattttt acaacactta ctcgggaggg
     1681 tattggaggg cattgcaaac cgggggttat aaagacggca aaaaacgcac acgcggtttc
     1741 tttcggaacg ggcggatgca aaacccgacc ctgcggcagg a
//
LOCUS       DAXHRI010000230         1773 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXHRI010000230 DAXHRI010000000
VERSION     DAXHRI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.230"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(540..1598)
                     /gene="alr"
                     /locus_tag="ACFM75_002052"
     CDS             complement(540..1598)
                     /gene="alr"
                     /locus_tag="ACFM75_002052"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC6256516.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWSAVGNQ
                     WQLEALLARHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT          444 a          450 c          401 g          478 t
ORIGIN      
        1 tccctgtgtc cttatttctg ctttcttacg tcattctttt aaaggtggga atctggaaac
       61 caaaaaacaa caggaattta tccgaaacaa caaaacctat ccgccggcat ccccgcgaaa
      121 gcgggaatct agtttgttgt tcaaataaat tctgaaactt taattttgtc attcccgcgc
      181 aggcgggaat ctagaaatga aaaacgacga gaatttatca gaaatgacgg aaactcaaaa
      241 aaccggattc ccgcctgcgc gggaatgacg aaattttagg tttctgattt tgattttccg
      301 tttttgcggg aaggatgggg tttcagattg tgggcattat cagtattttc tatttggcaa
      361 tgtcttttat aatggattaa atttaaacca gtacggcgtt gcctcgcctt gccgtacgat
      421 ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atactgattt
      481 tgtcaaaacg catttatgcc gtctgaaagg cttcagacgg catttttgtt tggacttgat
      541 tactcgatat aagtgaattt tgcacgtttg atattgcaca tcaattcgta agggatggtt
      601 ccggccgctt cggcaacggt attgatatta accgtatcgc cccacagttc gacctcgtgt
      661 cccaaacctt cttgcgaagc gtccagctcg atggtcatca tatccataga gaccctgccg
      721 atgacccggg tcaatttacc gtcgacggcg acgggggaat tgcttggggc gcggcgcgga
      781 taaccgtccg cataaccgca ggcaatcagg ccgacgcgcg tagatttact ggtataaaat
      841 gttgcgccat aaccgatagg ggagtgcggc tgtaaaacgc gttcgccgaa aatacgggtg
      901 gaaagcctca tcacgggttt cagcctgtca tcgctgcctc cgaacgggga aatgccgtac
      961 aacgccaagc cggctcgccc ccagtccctg cgtgcttcgg gaacattcaa aatagcggcg
     1021 gagttggcaa ggctttcttc gccttccagc ccttccgtac ccaaatcgaa tgcttccatc
     1081 tgtatttccg tcataccgct ttcgggttcg tccgcacagg agaaatgcga gaatttgaca
     1141 atactgtcca catattccga ctgcttcaat gccgcatatg ccgaagcgta atcatgaggg
     1201 aaaaatccgg tacggtgcat tcccgaatcc attttcaacc agaccttgac aggttttttc
     1261 caatggcggg ccagcaaagc ctcaagctgc cattggtttc cgactgccga ccagagcgag
     1321 tattgttcga ccgcttcgta ctccgatgct tcaaatacgc cttccaaaag gacaatcgga
     1381 tgggtaatgc cgctctcacg cagcctgatt ccctcatcga ttgtcgccac ggcaaagccg
     1441 tctgccaagt ctgccagcgc gaaagcacat ctgaccgcac cgtgtccgta tgcatccgcc
     1501 ttaactaccg ccaacagctt gcctccgtgc atttccttta aaatccgata attgtgccta
     1561 aggttgccca acctgatctg cacgttcaaa gggcgcataa attcattcct taccaaacaa
     1621 gtgtttatta gcaaaattaa gaaatcaatt agggaattat aatccaacgg caaggctttt
     1681 aaaatattaa agacccttgt gtcttcaagc cggagcagca gttttcaggt aggaaggcgg
     1741 tttgcttttc ctgatataat cgctttccca aat
//
LOCUS       DAXHRI010000231         1763 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXHRI010000231 DAXHRI010000000
VERSION     DAXHRI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.231"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(148..804)
                     /locus_tag="ACFM75_002053"
     CDS             complement(148..804)
                     /locus_tag="ACFM75_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6256517.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIRDYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALVAAAILLLS
                     "
     gene            869..1759
                     /locus_tag="ACFM75_002054"
     CDS             869..1759
                     /locus_tag="ACFM75_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC6256518.1"
                     /translation="MFPVFHLSGESRRRMLQAALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGVWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          389 a          419 c          583 g          372 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aaacggtttt ggacggaaaa acggcctgaa
       61 gccgtttcgg gcattcagac cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag
      121 ggcttcagac ggcattgatg tcgggtttca ggacaggagc aggatggcgg ctgcgacaag
      181 cgcggcaacc gataatgcgg cggcaagcgc ggttttgccg gcaaagcgga ttgaggtttt
      241 gccttcgatg tatttgaagc cggttatcat cgggaggacg aggtttttct ttttgaacac
      301 gcggtatgcg gcgacggcgg cgatatgaat ggcggaaaaa acggcgagca gcttgaaaaa
      361 gttgaggtgg attttccgca taaggctgcc tgtatgttcg gaaaccaaat ggttgaggta
      421 gccgttggtg ctgaaggtgt tttcatcggc ggcaaaaagc cccgtgccga cttgaaatga
      481 gacggcggcc aaaagcgcga cgaccatcag tgcgcccaag gggttgtgtc cgggctggat
      541 atgttcggga ataccgtttt tcagataatc cctgataccc gaccatccgc ggacgaaacg
      601 ggagaaacgg gcggtatcgc tgccccaaat gccccagcag aggcggaata cgagcaggaa
      661 aaggataaac agcccgacgc gcgtgtgcca ttgcagcata tcgccgccgg ttttcgcgct
      721 ataccacata aagggtaggg atgcggcaag cagccagtgg aaaaggcggg tggggaagtc
      781 ccagactttg gttttgtttt tcataatcgg tttccggcgg tagaatcggt ttgttttcga
      841 gccttaattt taaccgattg gaggggaaat gtttcccgtt ttccatcttt caggcgaaag
      901 ccgccgccgg atgcttcagg cggcattgcg ttttcctcat gttttcaaag cccgtgcgga
      961 agattcgcac aaagggactt tcggcacgct cgccgtagtc ggcggatcgg cagggatgag
     1021 cggcgcgccc gtattggcgg catcggcggc gatgtatctc ggctgcggca aggtgtgggc
     1081 gggtttcaat caggatgcgc ttccgtttgc cgtcattgcc ggttttcccg aaattatgtt
     1141 ggacacggca gacggtttga ccaagcgtca aggcataaac gcttggacgg cgggttgcgg
     1201 cttgggtacg gataaggcgg cggtcggaac gcttgccgga attttgacgg aacacacgga
     1261 caagcccgtc cttttggatg cggacgcgct gaacatatta tcaaccgatg ccgaaacccg
     1321 aaatctggca cgcgggtgta aaaacctgat tttaacgcca caccccgccg aagccgcgcg
     1381 cctgcttgga acgacggttg cacaggttca ggcggatcgg acggcggcag tgaggaagat
     1441 aggggcaatt ttcggcgcaa ccgtggtttt aaaggggcac aaaacattgg ttgccgcgtc
     1501 cgatacggaa atctatgtca acgaaagcgg caacgcggga ttggcaacgg cgggcagtgg
     1561 cgacgtattg ggcggcatca tcggcagtct gctcgcacag ggcgtgccgg tttttgaagc
     1621 cgcctgcgcg ggcgtgtggc tgcacggcgc ggcggcggat gtcataaaag aatcggcagg
     1681 cattgcggcg gggctgttgg ctggcgaaat cgccccggcg gcaaggtggc tgcgcaaccg
     1741 gataactgaa agtatgtaag aag
//
LOCUS       DAXHRI010000232         1732 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXHRI010000232 DAXHRI010000000
VERSION     DAXHRI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.232"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(44..367)
                     /locus_tag="ACFM75_002055"
     CDS             complement(44..367)
                     /locus_tag="ACFM75_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6256519.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(355..594)
                     /locus_tag="ACFM75_002056"
     CDS             complement(355..594)
                     /locus_tag="ACFM75_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC6256520.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(717..1514)
                     /gene="truA"
                     /locus_tag="ACFM75_002057"
     CDS             complement(717..1514)
                     /gene="truA"
                     /locus_tag="ACFM75_002057"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC6256521.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          380 a          408 c          506 g          438 t
ORIGIN      
        1 tgttaatcca ctataatgaa cacaatccat tcagactatt caatcaggca aacatctcct
       61 gcaatactgc aaacagtttt tcagccgtac tgttgtctaa atttccaaga tgtttgacca
      121 atccggcttt atccacagcc ctaatctgtt cgggcaaaag caaaccgtct ttatcctgaa
      181 agcggacatt gacgcggaac ggggcaggac ggcttccgct cgtcatggga acgatcagca
      241 cagtcttgag atagttgtgt atttcaggag gagagactac gacacaagga cgtgtctttt
      301 tgatttcgct tcctacggtc gggtctaagg agaccagata gattccgccg cgtactacca
      361 tatccattct ttatccgctt cgttttcaat ttcggaaaaa aaatgctcct gctcggtttc
      421 gacaagcatt gcggcagctt ctgcccatcc cctgcgaacg gtaggacagc ttaaaataat
      481 attgcccttt tcaactgtaa cagccaagct gtctactgcc cctatttgac ccaataatga
      541 tttgggcaga atcacgcctt gcgagtttcc cattttgcgt atgttgagaa tcatatacgt
      601 accctattta ccagtttatg taataacaat gttattacct tgatgaggta gtgtcaatat
      661 tgaaaaagat tgcctgacag tttgtccgat ttcaaaatct ccgcgacaag aatgttttaa
      721 agccattcgg ggatttgggg gcggatgatg ccgtatgcct cgggatagtc gacgccggtc
      781 aggtagagtc cgtcgggcat gaaggtcggt ggggctttga ggcggctgcg ttcttgaatc
      841 agtgcggcga agccttcgac gctgagtctg ccgctgccga cataaacgag cgcgcccatg
      901 atgttgcgta ccatatggtg caaaaaggcg ttgccgtgca aatcgaggcg gacgagtcct
      961 gagctttggg taaggtcggc gcggtagatg gttttgacgg gggattttgc ttggcattcg
     1021 gcggcgcgga agctggagaa gtcttgttcg ccgatcaaat aggcggcagc ccgacgcatc
     1081 ggttcgatgt cgagtttgag gtgtgtccag cctgccctgt ttttgagcag gggggaacgg
     1141 acgggggcgg attcgagcag gtagcggtag tgccgtccgt atgcgtcgaa gcgggcgtga
     1201 aattcggggg cgacctgtcg ggcgtgcaaa acggcaatgc cttcgggcag gtgggcattt
     1261 acgccgcgca cccatgcctg ttggggacgg gcggcagttg tgtcgaagtg gacgacttgg
     1321 gcggtggcat gcacgccggt gtcggtcctg ccggcaacgg tggtggaaac cgcttcccct
     1381 gctatttggg cgagcgcggt ttccaatgcc gcctgaacgg tcggtacgcc gtcagcctgt
     1441 ttctgccagc cgtaaaagcg gctgccgtca taggataggg ttattgccca gcgttgtttt
     1501 tgtgcggtat ccatcggatt tgggattcgg ataaatgttc agactgcatt gtatcgcaga
     1561 ttttgcaggg aaacggcaaa cgcccagggc gagcggcgtt gtttggggag ttgggggggg
     1621 gtggatgcag ttgctacgaa tcgctatcct gtgaattcac cctgtcagga gtgcccgaat
     1681 cgtcattccc gcgcagtcgg gaatctagga cgtaaaatct aaagaaacca tt
//
LOCUS       DAXHRI010000233         1705 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXHRI010000233 DAXHRI010000000
VERSION     DAXHRI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.233"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            52..1560
                     /gene="nadB"
                     /locus_tag="ACFM75_002058"
     CDS             52..1560
                     /gene="nadB"
                     /locus_tag="ACFM75_002058"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC6256522.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKLAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLTVQIRCRPNIRVCEQQMALDIQTES
                     GAACGLTVLDCRTQATYSIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYSLGETACTGLHGANRLASNSLLECVVTARLTAQT
                     IADGQAFQTATSQRSSENTSAEADIFSDDLQNAFSRPTLQAFNQRHLGIIRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT          409 a          570 c          429 g          297 t
ORIGIN      
        1 aatttaatcc actataccgt ttcagacgat ctctcaaaca aggacaacac catgcaaacc
       61 gattgcgacg tattgattgc cggaaacggg ctggcggcac tgacgctcgc cctgtcgctg
      121 cctgaatcgt tccgcatcgt cattttgtgc aaaaaccggc tggacgacac tgccagccgt
      181 catgcgcaag gcgggattgc ggcggcgtgg tcgggagagg acgacatcga aaaacacgtt
      241 gccgatacct tggaagcggg cgcgggtttg tgcgacgaag ccgccgtccg caccatcctg
      301 tcgcagggca aactggcaat cgaatggctg ctggcgcagg gcgtggcgtt cgaccaaaat
      361 aataacggcc tgcacctgac gcgcgaaggc gggcacacct gccgccgaat cgcccacgtc
      421 gccgactaca cgggcgaagc cgtcatgcag agcctgactg tccaaatacg ctgccgcccg
      481 aacatccgcg tttgcgagca gcagatggcg ttggacatcc aaaccgaatc aggtgcggcg
      541 tgcggactga ccgtcctcga ctgccgaacg caagcaacct acagcatccg cgcccgccat
      601 accgtactcg caggcggcgg cttgggacag atttacgccg ccaccaccac gccgcccgaa
      661 tgcacgggcg acgccatcgc catggcgata cgcgcaggct gcgcagtcga aaacctcgaa
      721 tttatccaat tccaccccac aggcttggca agatcgtctg aaaacggccg caccttcctg
      781 atttccgaag ccgtgcgcgg cgaaggcggc atcctgacca accaagcggg cgaacggttt
      841 atgccgtatt acgaccgccg cgccgaactc gcgccgcgcg acatcgttgc ccgcgccatc
      901 gccgccgaaa tcgccaaaca aacgcaagac ttcgtctcac tcgatatcag ccatcaaccc
      961 gcagcgttcg tccgccggca tttcccgtcc atccatcggc actgtttatc ccaatgcggc
     1021 ctggatatca cgcgccaagc catccccgtc cgccccgtgc aacactacac ctgcggcggc
     1081 atccaaaccg acccctgcgg cagaacttcc ctgccgcagc tctactcctt aggcgaaacc
     1141 gcctgcaccg gcttgcacgg agccaaccgc ctcgccagca actccctgct cgaatgtgtc
     1201 gttaccgcca gacttaccgc ccaaaccatc gcagacggac aagcattcca aaccgcaacg
     1261 tcccaaaggt cgtctgaaaa cacctccgcc gaagcagaca tcttttcaga cgacctccaa
     1321 aacgcattca gccgccccac cctgcaagcg ttcaaccaac gccatctggg catcatacgc
     1381 aacgataccg gcctacgctg cgccatcgcc cagctgcggc tttggaagca aaaccaaacc
     1441 gaaccgcaca ccgcgtccga atacgaaaac cgtaacctgc tcgaatgcag cctcgccgtc
     1501 gcccaagccg catacaggcg gagacagaac atcggcgcgc attttaatag tgattgttaa
     1561 ggttggctgg ttagaaaaaa tcgttcaaat gaattttaat agcatggtta agagattggc
     1621 attcagtttt tagggttgcg taagttgggt tatcaatctg ttctaaaagc agaaaggccg
     1681 agacctttgc aaaatccccc aaaat
//
LOCUS       DAXHRI010000234         1672 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXHRI010000234 DAXHRI010000000
VERSION     DAXHRI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.234"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..732
                     /locus_tag="ACFM75_002059"
     CDS             <1..732
                     /locus_tag="ACFM75_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFC6256523.1"
                     /translation="VTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEYV
                     QFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSS
                     QFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVY
                     QATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKW
                     QGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            763..1182
                     /locus_tag="ACFM75_002060"
     CDS             763..1182
                     /locus_tag="ACFM75_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256524.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            1449..>1672
                     /locus_tag="ACFM75_002061"
     CDS             1449..>1672
                     /locus_tag="ACFM75_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256525.1"
                     /translation="MVVAENVKREGKSCADSDSGSAHVKSVPFGTTHAPVCRSNIFSF
                     SLLGFSLCLAVGTANIAFADGIIADKAAPKT"
BASE COUNT          545 a          248 c          385 g          494 t
ORIGIN      
        1 gtaacgtctt cctacgatca tgtcattact gggacaaaaa acttcggaaa aaaagccagc
       61 gagcaacgac cgacgattgc ggttcaggcc ttgaagcagt tggggctgtc ggagcaggct
      121 gcggaatatg ttcagttctc tatagatttg ttcagtgtgg gtaaatcggg gggcggtata
      181 cctaaggcta agcctgtgtt tgatgcgaaa ccgagatggg aggttgatag gaagcttaat
      241 aaattgacaa ctcgtgagca ggtggagaaa aatgttcagg aaacgagaag aaggagtcag
      301 agtagtcagt ttaaagccca tgcgcaacga gaatgggaaa ataaaacagg gttagatttt
      361 aatcatttta taggtggtga tatcaataag aaaggcacag taacaggagg gcatagtcta
      421 acccgtggtg atgtacgggt gatacaacaa acctcggcac ctgataaaca tggggtttat
      481 caagcgacag tggaaattaa aaagcctgat ggaagttggg aggtgaaaac gaaaaaaggt
      541 gggaaagtga tgaccaagca caccatgttc ccaaaagatt gggatgaggc tagaattagg
      601 gctgaagtta cttcggcttg ggaaagtaga ataatgctta aggataataa atggcagggt
      661 acaagtaaat cgggtattaa aatagaagga tttaccgaac ctaatagaac agcatatccc
      721 atttatgaat agtaatattt atgaaaaatt aggagattaa tgatgaaaag aattaagtgc
      781 ttttgtgata aatttccatc aggagataca tttagaatgt gtatcattct ggatgactat
      841 gataataggg ttgattatta tgtaggaata tatgattaca ttacgtctac cttaatgagc
      901 gatatttact atcgatccac gattgatgag catttcaaga ttatagaatt aatagaaaat
      961 aatccaaatg aaatttatga tgatggcggt ggtcaacaat tttgcctaga atttcatcat
     1021 gataaggtca ttttttacca caatgaattt gatgaagaag atggttatcc agtattaagc
     1081 tgttcgctgc atacttttaa aactgcttta attgcttgga atgctttttt gcaattgcct
     1141 aaaagtattc attcggtggt ggagactgtg attgaggaat aagcataatt agcttaatga
     1201 atagaatcag cgatatagat tggactgcaa atccacgctt atacgctgtg ccatgattaa
     1261 gatgttagaa cttgtattga atacaagttc tcataaacga atggcagtaa gcatttgatt
     1321 tagataaaat ccttgaatta gaataattaa gattatctat tttttaataa ctctctaaaa
     1381 atcaaggaac taaaatcata aacaaaaccc tctatcgtat catcttcaac tcttgtcgcg
     1441 gttgcctgat ggttgttgca gagaatgtga agcgcgaggg taaaagctgt gccgatagtg
     1501 attcaggcag cgctcatgtg aaatctgttc cttttggtac tactcatgca cctgtttgtc
     1561 gttcaaatat cttttctttt tctttattgg gcttttcttt atgtttggct gtaggtacgg
     1621 ccaatattgc ttttgctgat ggcattattg ctgataaagc tgctcctaaa ac
//
LOCUS       DAXHRI010000235         1659 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXHRI010000235 DAXHRI010000000
VERSION     DAXHRI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.235"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(1..1148)
                     /gene="pilC"
                     /locus_tag="ACFM75_002062"
                     /pseudo
     CDS             complement(1..1148)
                     /gene="pilC"
                     /locus_tag="ACFM75_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
BASE COUNT          341 a          435 c          448 g          435 t
ORIGIN      
        1 cttttctttt cagattttga aggaaaatgc ggctgctcga actgtcgggg taggtagaaa
       61 ccgaagcgga atacatctgc gccttaccat tttgcacagg tccgaaccac agcgcggggg
      121 cagtcagtgc gggcgaaggg gctttggatt tgggattggg gttcggttta ttgatgcagc
      181 ggcctgcttt gacttgctgc aggccgagct tgactggaat ctgcccgtta tctttaatgt
      241 cccacttagc ccaaaaactt agccagttgg caccttgccg ctgccgcaca tcgatgctcg
      301 gcttcaagac acccaactgc actgttttgt cggtgatatc tttaatccct tttttgtcca
      361 gcaggtgcag tttggcgttc agataatagg ccatggcggt atgttcgcgc tgcccgtcgc
      421 caagggaaat agggtggcct tctgtcgtag agacgatggt ttgattatta ttgtcttcac
      481 taaaagaaga tatcaagctg ctggatgatt ggctattttt gacggtattc ttgctgccca
      541 aattgaaaga tacgcccaac catggataat ctttcaattt gtaaatgggc gaaccgtcgc
      601 ggcttttgtc ttcgtatata tccagcctgc cgtctgtttt cttttttgac aacccggaat
      661 gaccgaagga aaatcgggtt ttatagacaa gttggggaca gcctgtgttg cttctgcata
      721 tgacgttggc gtagctgtag cctgccagcg cgatgcgggt ggtacgaatc caaccggcgg
      781 cattgttgcg cttttccagc ccgtcggcat caaaaccgga aaccttgccg tagggcggca
      841 ggtaggtggc tgtgccaaaa acggcagtac cgctttgtcg ggaaacaagg gtatcggtat
      901 tgtcgaaaaa gacagagcct ccacccgttc cgcctttatt atagataaat ttgcgttccc
      961 tgtctttttc ctttaaggtt gaatatgaac cctcctgctt taccttcggc tgatttcgtt
     1021 cgtccatgac aatagcgtag ttatgggttt ccgcctgcgc cccccccccc gccggtatgg
     1081 gaaaacatca agatggcggc ataaagcgcg gtatggcgga aaacccgcct ttttaaagtt
     1141 ttattcatcg tatttccttt tcggtcaaac cccccgccct tcgggcatcc gtccttcggg
     1201 gcggcaggat caggctttgt ccgggagggg tgcaagccct tccgaatcag ggcggcgcct
     1261 tatgtgccgc cctgcgtgtt ggaacatatt ttatccctta aatcttgctt cgcgggatac
     1321 cgtcggcggc aggcgcgccg cgaacgccgg ggttcggtaa accgcccgat tccgcgcccg
     1381 ccgaattgct ggttggaaaa cccctttggc ccggctgcca aagggggatg ttcggaaaag
     1441 taacacgccc ctttggccgg atgcaatgta tagggttacc gcattattct acccttgcgc
     1501 gctgatcatg gtaaagcggt tttcggcaat ccgcaaccgc ctccttggcc cgccattgcc
     1561 gcgaaagcgg gaatccggaa cgtaaaatct caaaaaaacc gttttatccg gcaagtttgc
     1621 ccgccggcag gcccgccatt gccgcctgcg cggcaatgg
//
LOCUS       DAXHRI010000236         1652 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXHRI010000236 DAXHRI010000000
VERSION     DAXHRI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.236"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(48..710)
                     /locus_tag="ACFM75_002063"
     CDS             complement(48..710)
                     /locus_tag="ACFM75_002063"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6256526.1"
                     /translation="MIHAVLFDLDGTLADTARDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(723..1550)
                     /locus_tag="ACFM75_002064"
     CDS             complement(723..1550)
                     /locus_tag="ACFM75_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC6256527.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAINLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVCHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGHVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          309 a          438 c          508 g          397 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatgtt tgtggggcgg atatttttca gggctgtatt
       61 ttgtccagac attcgagcag atcgagtggc gtgcggatgt ggaaatccgc ctgccatgag
      121 ccggtatcgt cttcgggagc gatgtagccc cattcggcga ggacggtttt catgccggcg
      181 ttgcggcctg cttggatgtc gcgttcggca tcaccgacgt agagtgtgtg ttgcgggtcg
      241 gcgtggattt gtccgcacgc atacagcatg ggtttgatgc tgggcttggg ttcgccgcag
      301 gtgtcgccgc tgacgacgac ggcgggtggg atgatgaagc cgagtttggg gacgagtttg
      361 tcggtgaagc gcatgggttt gttggtgatg atgccccatt tgatgccgcg ttggttgagt
      421 tcggcaatga gttcgttcac gccgtcaaag agctcggtgt cttgggcgta gcggcggtcg
      481 tattcctcca gatattcggt gcgccatcgg gcatagtcgg ggtggtcggg ggtgatgcct
      541 gcgccgagtt tgatcagtcc tgccgcgccg tggctggctt gggtgcggat ctcgtccatg
      601 ctttttgcag gtagtccgtg gcgagcgagc agggtgttga gtgcgcctcc gaggtcgcgg
      661 gcggtgtcgg caagtgtgcc gtcgaggtcg aagagtacgg cgtgtatcat gtgtgttcct
      721 tttcagacgg tatttttgtt ctgtttcaaa ccctgctcga aatcttccag catatccaat
      781 tcaaagcggc tgaagcccgc tttttcgcgc gcttcgatgt tcacatggcc gcggaagata
      841 aacatatcgt aacgggcaat cagactgcgg aacagggcga caggttccaa accgcgttcg
      901 cggcagaggt gttgatacca atggttgccg atggcgacat ggcccacttc gtcgcggtaa
      961 atgatgtcca acacgccgca ggtttccgaa tcgccgcgct gctccacctt cgcgcgtatc
     1021 cccggcgtaa cgtccagccc gcgcgcctcc aacacgcgcg gtaccaacgc catacgcaac
     1081 agcgggtcgt aggcggtttt gtacgccata tcccacaaat ggttgtgcgc ctcgaaactg
     1141 ccgtaatcga agccgaaagc gcgcaatctg tcgcgcatca ggcggaagtg gcacacctct
     1201 tccttcgcca ctttcaccca gtcgcggaca aactgaaacg gcagcgtgcg gaaacgatat
     1261 gccgcgtcca aagccagatt gatggcgttg aattcgatat gcgcaatcgc gtgcagcatc
     1321 gccgcatagc cttcggttgt gttcattttg cgcggtgtca gctgcgacgg cgcgaccaaa
     1381 acaggcttgt ccggtcgtcc cgcgcggggg aaatctgccg gtggcgcgtt tgtttccgcc
     1441 ccgtccgcat tttgaacggc ggcaaacgcc tcatccgtca gccgtccttt ttcatctggg
     1501 tcgcccgaaa gcagggcgcg ttccagcaaa gcataaatat cgggtttcat ttcaagtccg
     1561 ccgtgttcgg aaaacaaata ttatagcgtt taaaaaaaac aagatgaggc atataatctc
     1621 cgcgattcgg cattccgcgc ccaaaccgtc aa
//
LOCUS       DAXHRI010000237         1647 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXHRI010000237 DAXHRI010000000
VERSION     DAXHRI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.237"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            1..171
                     /locus_tag="ACFM75_002065"
                     /pseudo
     CDS             1..171
                     /locus_tag="ACFM75_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
     gene            211..594
                     /locus_tag="ACFM75_002066"
     CDS             211..594
                     /locus_tag="ACFM75_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC6256528.1"
                     /translation="MYCLRLRRLVLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETE
                     GLGLNVISHKILGKDHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPA
                     MKQPCICGSGKKFKACCGKYLKPVA"
     gene            complement(812..1606)
                     /locus_tag="ACFM75_002067"
     CDS             complement(812..1606)
                     /locus_tag="ACFM75_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6256529.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTVRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          424 a          495 c          366 g          362 t
ORIGIN      
        1 agctatgtcg gctgctgccg cccattgcac cttcgccata tcctaccgcc caccgctgaa
       61 gccctcatgc gttcgcggta cagcgcgtat gtgctgcacc ttatcgacta tagtggatta
      121 acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga gaacgattct ctaagatgct
      181 gaagcaccga gtgaatcggt tccgtactat ttgtactgtc ttcggcttcg tcgccttgtc
      241 ctgatttttg ttaatccact atacatcatc gctactaccg ttccggcgca acaggcattc
      301 ctcgatgccg ccgaactgat gcaatggagt atagaaaccg aagggctggg cttgaacgtc
      361 atctcgcaca agatactcgg caaagaccac gcccaagtcg aatttgaagc ctacttccga
      421 gacggacaac accgatccgc gcatcacgaa ctgtccggct tcgtcaacat cggcggacaa
      481 tggtatttta tcgatccgac cgttccgcat cctgcgatga aacaaccctg catttgcgga
      541 tcgggcaaaa aatttaaagc ctgctgcggc aaatatctga aacctgtcgc ataaaaatgc
      601 cgtctgaacg ttcagacggc atttttaacg cgcaaaaaaa aaccattcat accaagggta
      661 agtatgaatg gtcaatacat tgcgggaaaa cgtcttactt gctgcactgc cgaaaaggga
      721 gaaacggcag cggtaatcag cggaaaggat tgcacccgaa ttaatgttaa gaaacgttaa
      781 ttgtgaaaat atattaacaa acctgttgaa cctattggtt ttcccgtatc cacccgaccc
      841 agcgttcaaa cagcttcggt tcgagcgcgg caacgaccga gcgtttgaac acgtgttcac
      901 cactccaaaa cccgtcgcct tccaaagtcg tcagcctgcc gcccgcctcc tcgaaaatca
      961 acgcacccgc agcatagtcc cacagcttct gcccgccgtg gacataaaca tcataacgcc
     1021 cgcacgccag ataacaccag tccagcgtgc tgctgcccat actccgcacc gtaccgaaag
     1081 gtgcgagcgt actcatgcgg ctggaaagtt tgcccgaacg cagatatttg atttccacgc
     1141 ccgcaatcgc ctcattgagt tttttatcca cgagacgcaa aggcaggcgg gttccattaa
     1201 gaaacgcacc ctgcccccgt tcggcataaa aacattcgcc gctgaccgga ttgtaaatca
     1261 cacccaattc ggcgcgcccg ttgcggacaa acgccaccga cacggcaaaa tgcggcagcc
     1321 cgttgacaaa attgttcgtc ccgtctatcg gatcgacaat ccacagcccc ttttcccccg
     1381 aatattgttc ccacaaagcc gactgttcct gccgcgacat ttcctcgccc aacatcggac
     1441 tgtcgattaa aagcggcaac gcggcggcaa aagccgtctg cgcggcaatg tccgcctcgc
     1501 tcaacatcga accgtcttcc ttgcggtgag acggcgtatt caaaaaacgc ggcataattt
     1561 cggtttgcgc gatatggcgc acgactttct gcaaacggtg taacacttcc tactgtcctc
     1621 atattttgaa cttgcggcac gcgaacg
//
LOCUS       DAXHRI010000238         1641 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXHRI010000238 DAXHRI010000000
VERSION     DAXHRI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.238"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..289)
                     /locus_tag="ACFM75_002068"
     CDS             complement(<1..289)
                     /locus_tag="ACFM75_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256530.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSDGFLKIPHGRKV"
     gene            complement(303..776)
                     /locus_tag="ACFM75_002069"
     CDS             complement(303..776)
                     /locus_tag="ACFM75_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC6256531.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(829..1110)
                     /locus_tag="ACFM75_002070"
     CDS             complement(829..1110)
                     /locus_tag="ACFM75_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256532.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKMDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(1197..1520)
                     /locus_tag="ACFM75_002071"
     CDS             complement(1197..1520)
                     /locus_tag="ACFM75_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256533.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
BASE COUNT          469 a          425 c          252 g          495 t
ORIGIN      
        1 gcacttttct cccgtgtgga atttttaaaa aaccatcact cttgacgatt tcatgcagct
       61 cctcacttga tttctccgca tattccattt tcaacgcctt atgctcattg ccggcttcaa
      121 tcattccctc tcccactgct tttgccacac cattccaaaa acccataatc atgtcctcca
      181 atcgtttcaa ataaataagg tcgttgtttc tgtcaagaaa caacgacagt ttataaactg
      241 tcggaaaaca aaatgccgtc tgaaggccgg ataatgcttc agacggcatc ggattgccgg
      301 ttttaaccct gccctccgcc gttgttcaaa tagtgttccc agtgagaata taaccgggca
      361 atattttctt cataggtgtg ggcagaagga atataaccgt ctttgggcgg catatacctg
      421 acaaatgatt ctatttcttc cggataatca aaatctgcat aaattttttc aacttccccc
      481 aaaggatcct caaattcttc ttttctatgg aatacatcgc ttaagaggac gtacagccaa
      541 tttttagggg aataatccct catttccgaa gccaaatctt ccagaacagg ctttaattca
      601 agaatattgg atttgttgat taaagatagt ttgaacacac gttcatcatg atctgaaagc
      661 gtcatttttc gggcataagc tgccacatca gcccaaccca agtatttatt cccatacccc
      721 cacagaatat ccttccaaga aagacggact ttttgcgcgg ttaaatcaag gttcattttt
      781 tactccgatt gcctgccccg tcattccgcc ccttccgatt gacgcctgtt acgaaaagtc
      841 ttccaaatga cggctgattg cctcttcaac agtcggatgc caatccataa atgaagcctg
      901 caattcctct aaaaccaact cattgaataa atcatccaaa ttccgccaat ccattttttc
      961 atccggatag atgaaatttg ccataacgac agtatgtttg ttttgggaat aatccgataa
     1021 ggcgcaaatc atcagatacc gccccgaatg ctccagataa aaaattcgtt tttccaaagg
     1081 aaaccggtta ttccgttgcc cgttttccat ttttcatcct ttttaacgta tcggcttccg
     1141 cgtccttcaa attgagttac ctgccttccg ccaagcaaac gcccgcgccc ctttcatcat
     1201 ttgaatatcc gtttgaccat atttaccaga taactatctt ctttatataa atcaatcagc
     1261 aaaggcacga tacccggctc ataaaataat tctgtcgtat cagatgtttc ttttaacgcg
     1321 gaaattatct ccaacagttc atacccgtat cggggataag caaacaaata ggggactgct
     1381 tcccgcaaac atgaaaatgc ttcgtaactt tgcatttccc atatatgttc atctttaaaa
     1441 tcactcaaat accattcatc caattcactt tccggatcgg atatcaatcg gataaattca
     1501 tttagttcgt ccttaaacat atcaaatcct taaatataaa atctgagacc tttgcaaaat
     1561 tcctttccct cccgacagcc gaaaccccaa cacaggtttt cggctgtttt cgccccaaat
     1621 accgcctaat tctacccaaa t
//
LOCUS       DAXHRI010000239         1637 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXHRI010000239 DAXHRI010000000
VERSION     DAXHRI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.239"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..199
                     /locus_tag="ACFM75_002072"
     CDS             <1..199
                     /locus_tag="ACFM75_002072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256534.1"
                     /translation="DVFGNHGLGGLLKEGAHEKSPKCLGGNLGDFGEFCKGLSLFKPQ
                     PFPTKPKGRLNPIFRRPFATL"
     gene            284..496
                     /locus_tag="ACFM75_002073"
     CDS             284..496
                     /locus_tag="ACFM75_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HFC6256535.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            680..951
                     /locus_tag="ACFM75_002074"
                     /pseudo
     CDS             680..951
                     /locus_tag="ACFM75_002074"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(1041..1490)
                     /locus_tag="ACFM75_002075"
     CDS             complement(1041..1490)
                     /locus_tag="ACFM75_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6256536.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          501 a          381 c          276 g          479 t
ORIGIN      
        1 cgatgtgttt ggcaatcatg gcttgggcgg tttgctgaaa gaaggtgctc atgagaaatc
       61 tcctaaatgt cttggtggga atttagggga ttttggggag ttttgcaaag gtctcagtct
      121 ttttaaacca cagcccttcc caactaaacc aaaaggtcgt ctgaacccta ttttcagacg
      181 accttttgcc actttgtaaa acaaatcttc ccaccatcct ctccccaaac atcgcccgaa
      241 ccagtaaact tctcatattt caacaacttc ttggaagcaa accatgcccc atatccaccc
      301 gctccacctg ttcccgcccc atatcgccga acgcggactg ctctattttc agcagggcaa
      361 agttggtgct gtccaaaaaa cttccaccgg gcgttatcgg gcggaggtat gcggtttgga
      421 aaactattgg gtatggctga agctggatag tgatttgtat attaaagacg aaagctgcga
      481 ttgcccttat gtctaaaggt gcaaacatac cttaaattac tatattacat aggcaaaata
      541 caagcctata acgaattgga aacaaaatgc cgtctgaaag cattataaaa tctgttcacc
      601 ttttcagatg agtaatgtac acccctatac aatttttgct actatacccc ataaatccac
      661 ggctaaagat atccttatta tgtcctatga tttatcgaaa cgacttgtaa tcggcttagc
      721 atcaagtgcc ctattcgact tatccgaatc ggataatata tttagaatgg aaggggcaga
      781 aacctatagg caatatcaga gaaaaaaaca aaaccatccc ctaaaaaagg cgttgtcttt
      841 ccatttatta aaaaacttct gtcaatcaat gaaataaacc caaacgaccc aacgattggg
      901 tttattattc ttttatccag aaacaatcca gatacagatt acgagtcata actataggct
      961 taatattaca cgattttcat tccatcaagg cggaaaaccg cacaaatact gaaacactat
     1021 cgatcgattt gtaaacaagc ctacttaagt aacttgcagt ccttatcatt tcctttaaaa
     1081 taatccagcc cgtcactaca cgaactggcg gacttcttgc aaataaaggt tactagattt
     1141 tcattcatct taataataaa aggattttta tctttatcta tggctaccgc cttcaacatg
     1201 aatttactgt ctaaggcccc gcgcgcgatt ccattcaaac ggatacaaaa gccttctgcc
     1261 tctttaatcg gcaaacttgg ccatttggta gatgtttgtt taaatctccc attctgcaga
     1321 taaaactttt ccataaaatg tgcattttct aacaaggctg cccgcactgc atttatcttt
     1381 gctttctcaa cataattgcg atagctcgga taaacaatta aagcaagtac agacaatatc
     1441 aagaccactg atattaattc aaccagcgta aacccccgat tatcagtcat tactttactt
     1501 ccaataagaa cagattattc aacatatttc tttgaacaga cttactatcc cattcagcag
     1561 tatgcatatt tcccactcta ttttttatcc gcgggcagca ctggtttgtc tgggcctttt
     1621 ggtgcgggtg cgccgac
//
LOCUS       DAXHRI010000240         1625 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXHRI010000240 DAXHRI010000000
VERSION     DAXHRI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.240"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            56..1225
                     /gene="metZ"
                     /locus_tag="ACFM75_002076"
     CDS             56..1225
                     /gene="metZ"
                     /locus_tag="ACFM75_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC6256537.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1320..1526
                     /locus_tag="ACFM75_002077"
     CDS             1320..1526
                     /locus_tag="ACFM75_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC6256538.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          392 a          426 c          433 g          374 t
ORIGIN      
        1 attaaacaac gggcgcacgc tccgttgccg aacggataaa accgaggaat caccgatgag
       61 caaaaaactc catccgcaaa cgctcgccat acgcggcggc aaagaacaaa ccgaataccg
      121 cgaacacaat caggcattgt tcctgaccag cagcttcatg tgggacaacg cccaacacgc
      181 tgccgatttg ttttcaaaaa aaatcaaagg cttcacttat acccgtaccg ccaacccgac
      241 cacagccgcc tttgaaaaac gcatcgccgc tttggaaggt gcggaacgcg cggtcgccac
      301 ttcgaccggc atgtccgcga ttcaggcggc gtttttcacc tttttgcagg cgggcgacca
      361 tgtgatttcc agccgcagcc tgttcggcac gaccgtcggc tttatcaata acatcgttac
      421 caaattcggc atcgaagtga gtcacgtgtc gccgactgat ataaacgaat ggaaagccgc
      481 cgtcaaagcc aacaccaaac tgctgttttt ggaaacgccg tccaatccct tgggagaagt
      541 ggccgacttg gaagccttgg cggaattggc gcacggcatc ggcgcgctct tggtggtgga
      601 caacagcctg ctgtcgcctg tcggctctca gcctttgaaa cacggcgcgg atatttccgt
      661 ttcctccgcg accaaagcca tagacggaca cgggcgcgtg atgggcggcg tgttggcggg
      721 ttcggaagaa ctgatggcgc aggtcgccgt ttactgcaac tcttgcgggc tggcaatgtc
      781 gccgtttaac gcatggcagt tgttgagcgg cgtggaaacc ctgtctctgc gtatggaaaa
      841 acagttcgac aacgccttga aaatcgcgca atggctgcaa gcgcagccgc aggttcaagc
      901 cgtgtattac accggattac ccgaccatcc tcaggcggag cttatccgca aacagcaaaa
      961 cggcggcggc atcgtcatcg gctttgaagt cgccgaccaa gcagccgcgt ggaaagtcgt
     1021 ggacggcgtc gaacttttct cccgtaccgc caacctcggc gacgtgcgct cgaccattac
     1081 ccatccgtgg acaaccacgc acggcaggat gcagcccgaa gaaaaactcg ccgccaacat
     1141 ccgccccgga ttggtgcgcc tgtcggtcgg tttggaatat gttggcgatt tgattgacga
     1201 tttgaaacag gcactcgcgc gttgattgcg tgttcagacg gcatcggcgg aaaaacaccg
     1261 caaatgccgt ctgaaaccct tatcttatat gacatacacg tcaacaatag gaaaaaaata
     1321 tggatgaagc ggtttttgcc gattgggcgt tgaaaatctg cctgaccggc ttgattattt
     1381 ttttgggttt tatcgtttgg aatttgggca aggagtctaa agcgggcaaa ttcggcatcg
     1441 ccattttgtt tttggttttg ggcttgggcg tgtttggttt tgtgttcaaa gagttattga
     1501 ttaagttttt ggtattgccg aaataggttt gcggtctgtc aaaatgccgt ctgaaatcat
     1561 aaaaagtaat ctgcacccta aaagtttgat ttaaccgcca actgattaag gtgcggattt
     1621 ttatg
//
LOCUS       DAXHRI010000241         1622 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXHRI010000241 DAXHRI010000000
VERSION     DAXHRI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.241"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            114..1487
                     /locus_tag="ACFM75_002078"
     CDS             114..1487
                     /locus_tag="ACFM75_002078"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6256539.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          416 a          530 c          389 g          287 t
ORIGIN      
        1 tgtactgtct gcggctcgcc gccttgtcct gatttttgtt aatccactat atttccccgt
       61 cctatcggtt tcccgtttca gacgacataa ggtctgaaag aaagactaca attatgagta
      121 atccattttc ctctttaggt ttgggtacgg aactcgtttc cgcactgacc gcgcaaggtt
      181 acgaaaaccc gacgcccatc caagccgccg ccattcccaa agcactcgcc ggtcatgatt
      241 tgctagccgc cgcgcaaacc ggcacaggca aaaccgccgc ctttatgctg cccagtctgg
      301 aacgcctcaa acgttacgcc accgccagca cctcgcccgc gatgcacccc gtgcgtatgc
      361 tcgtccttac ccccacgcgc gaacttgccg accaaatcga ccaaaacgtg cagtcctaca
      421 tcaaaaacct gccgcttcgc cacaccgtct tgttcggcgg tatgaatatg gacaaacaga
      481 ccgccgattt gcgtgccggc tgcgaaatcg tcgtcgccac cgtcggacgg ctgctcgacc
      541 acgtcaaaca aaaaaacatc agtttgaaca aagtcgaaat cgtcgttctg gacgaagcag
      601 accgcatgtt ggatatgggg tttatcgacg acatccgcaa aatcatgcag atgctgccca
      661 aacaacgcca aaccctgctc ttttccgcca ccttctccgc accgatacgc aaactggcgc
      721 aagacttcat gaacgcgccc gaaaccgtcg aagtcgccgc gcaaaacacc accaacgcca
      781 acgtcgaaca acacatcatc gccgtcgata ccttccaaaa gcgcaacctg ctcgaacggc
      841 tgattgtcga tctgcatatg aaccaggtca tcgtgttctg caaaaccaaa caaagcgtcg
      901 accgcgtaac gcgcgaactg gtgcgccgca acctgtccgc acaggcgata cacggagacc
      961 gttcccaaca aagccggctc gaaacactca acgccttcaa agacggcagc ctgcgcgtcc
     1021 tcgtcgccac cgacatcgcc gcgcgggggc tggacattgc cgaactgcct ttcgtcatca
     1081 attacgaaat gcccgcccag cccgaagact acgtccaccg catcgggcgc acggggcgcg
     1141 cgggcgcgga cggcgtggcg atttccctga tggacgaatc cgaacagaaa atgtttgaat
     1201 ccattaaaga gctgaccggc aacaagctgc tcatcgagcg catcgagggc ttcgagccgc
     1261 aatggtggga acagggcggc gcaaaaccgg aaaaaaccga aacaagcgaa ccgagacaac
     1321 gcaaccgcta cgaatccgcc agggctcagc gcgaaaaaaa tacccgaccg gaaaccacgg
     1381 caaacgatgc gggcgcggct tgcggaaaaa ttgccggacg cagccgccga agccgtcggg
     1441 aacaccggac gtgcgccctg ctccaaccgc gttacggcgt aaaataaccc tgaaaatcaa
     1501 atgccgtctg aacatttccc gtttcagacg gcatttttca aaccggactg acgcatcggg
     1561 agcaaccgcc cgcaccggat aaatttcttc cgcaaacagt ttcagacggc atttgccgcc
     1621 tg
//
LOCUS       DAXHRI010000242         1562 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXHRI010000242 DAXHRI010000000
VERSION     DAXHRI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.242"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..439
                     /locus_tag="ACFM75_002079"
     CDS             <1..439
                     /locus_tag="ACFM75_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6256540.1"
                     /translation="DKQIDDHTHTHFDGKDQVAEQIKGIGSITTATLMAMLPELGRLS
                     HKRIAGLAGIAPHPREGGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFHQRP
                     LSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
     gene            596..1030
                     /locus_tag="ACFM75_002080"
     CDS             596..1030
                     /locus_tag="ACFM75_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC6256541.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
     gene            1011..1439
                     /locus_tag="ACFM75_002081"
     CDS             1011..1439
                     /locus_tag="ACFM75_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6256542.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
BASE COUNT          400 a          314 c          465 g          383 t
ORIGIN      
        1 ggacaaacaa atcgacgacc acacccacac gcattttgac ggcaaagacc aagtggcgga
       61 gcaaatcaaa ggcatcggtt cgataacgac ggctacgctg atggcgatgt tgcccgaatt
      121 ggggcggctg tcgcacaaac ggatagcggg tttggccggc attgccccgc acccgaggga
      181 gggcggggaa accaaattca aaagccgctg ctttggcgga aggtctgcgg tgcgtaaagc
      241 actgtatatg gctaccgtgg cagcgacacg ttttgaaccg cttattcggg atttccacca
      301 acgcccgctg tccgagggta agccgtataa ggttgccgtt acggcatgta tgcgcaaact
      361 gctgacgata tcgaatgccc ggatgcgtga ttattttgcc gaaaacgata ccgccgaaaa
      421 cggtatccgg acggcttgat ttgagttttg gtatttttgc ccgacggggt gaaaaataca
      481 gttgcttttt catgtctgtc cgtttcgcaa aaaaaatcgg cttaatacta tatattgtat
      541 tttattggta taatatgcgt taattagtca atatattgtg ttttatgggt ttgagatgcg
      601 ccgggcgttg attgcaaaaa ttaaaattgc ccaaaaggag ctgggcttgg acgacggtac
      661 ctatcgcgcg gtgttggagc gcgtgacggg caagcggtcg tgtgcggata tggatgtttc
      721 cgaacttgag tctgttgtcg ctgatatgcg gtcgcacgga tttaagccta aagcaaaagg
      781 taacccacac ggtaaaccac atctgcgtcg gacatcatca gcggcaatgt tggacaaagt
      841 cgaagccctg ctgaccgtcg gcggcaaaca ttggaactat gcacacgcaa tggcgcggcg
      901 gatgtttggt aaggataagg tcgaatattt agacgatacg cagctacata aactggttgc
      961 tgcgttgcag attgcggaaa acaggaaaac ggaaaaagcg ggtggggatg atggggttcg
     1021 aaaaagttga acatttattg ccggataccg tgttggacat tgtggatgtc atcggactgg
     1081 cagcgacgga acagctggtc aaggcgattg gcggggcgcg gtttaaattt ggtaagggca
     1141 aggtggacac cgagcgtttg gcaattttgg tcgaagccat cggcgaagtg aaaacacatg
     1201 agctgttgca ggtatatggt ggcgaggaat tgtatgtccc acggtgcggc aaggcgttaa
     1261 tacagttgag aaaccatagg ttttatcagg agtttgtcaa attgcgcgat attgataagg
     1321 agagcgggct tatggcgatg acgaagctat gccctaaata cggcatctct tcacgaacgg
     1381 gatatacgat tatcaatgaa atgagccgac ctgcggcaca gcaggcagct ttattttagg
     1441 cagtgatgtg tgaccaggct ttggccgtct gtattcagac ggtctttttt ttggtttgca
     1501 ggggtgaaac atctaccgtt cgggacgatg ggttaaagac ggtttaatgg ggttttcaaa
     1561 tg
//
LOCUS       DAXHRI010000243         1524 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXHRI010000243 DAXHRI010000000
VERSION     DAXHRI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.243"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            34..1425
                     /locus_tag="ACFM75_002082"
     CDS             34..1425
                     /locus_tag="ACFM75_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC6256543.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIMEKAKALKETPPNPTKAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWSTML
                     AANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKEK
                     TVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSLP
                     DGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTTYRTAKASGNDVDSKGASSARFKNH
                     ADIIGLQYTYKFK"
BASE COUNT          407 a          491 c          358 g          268 t
ORIGIN      
        1 ttatccaccg tccgcctttt tacggaaacc gaaatgaccc cttccgcact gaaaaaaacc
       61 gtcctgctgc tcggcactgc ctttgccgcc gcatccgcac aagcctccgg ctaccacttc
      121 ggcacacagt cggtcaacgc gcaaagcacg gcaaatgccg ccgccgcaga agccgccgac
      181 gcatcgacca tcttctacaa ccctgccggc ctgaccaaac tcgacagcag ccagatttcc
      241 gtcaacgcca acatcgtgct gcccagcatt cattatgagg cggattccgc caccgacttt
      301 accgggcttc ccgtccaagg ttcgaaaagc ggcaaaatca ccaaaaccac ggtcgcgccc
      361 cacatctacg gcgcatacaa agtcaacgac aatctgaccg tgggcttggg cgtgtacgtc
      421 cccttcggtt ctgccaccga atacgaaaaa gattccgtgt tgcgccacaa catcaacaaa
      481 ctcggtctga ccagcatcgc cgtcgaacct gtcgccgcgt ggaaactcaa cgaccgccat
      541 tccttcggcg caggcatcat cgcccaacat acttccgccg agctgcgcaa atatgccgac
      601 tgggggatta tggaaaaagc gaaagcacta aaagaaacac cccccaatcc aactaaagcc
      661 gcccaaatca aagccgacgg acacgccgat gtcaaaggca gcgattgggg cttcggctac
      721 caactggcgt ggatgtggga catcaacgac cgtgcgcgcg tgggcgtgaa ctaccgttcc
      781 aaagtctcgc acacgctcaa aggcgatgcc gaatgggcgg cagacggcgc ggcggcgaaa
      841 gcaatgtgga gtacgatgct tgcagcaaac ggctacacgg cgaatgaaaa agcccgcgtt
      901 aaaatcgtta cgcctgagtc tttgtccgta cacggtatgt acaaagtgtc cgataaagcc
      961 gacctgttcg gcgacgtaac ttggacgcgc cacagccgct tcgataaggc ggaactggtt
     1021 tttgaaaaag aaaaaaccgt cgtcaaaggc aaatccgacc gcaccaccat cacccccaac
     1081 tggcgcaaca cctacaaagt cggcttcggc ggttcttatc aaatcagcga accgctgcaa
     1141 ctgcgcgccg gcatcgcttt tgacaaatcg cccgtccgca acgccgacta ccgcatgaac
     1201 agcctgcccg acggcaaccg catctggttc tccgccggta tgaaatacca tatcggtaaa
     1261 aaccacgtcg tcgatgccgc ctacacccac atccacatca acgacaccac ctaccgcacg
     1321 gcgaaggcaa gcggcaacga tgtggacagc aaaggcgcgt cttccgcacg tttcaaaaac
     1381 cacgccgaca tcatcggcct gcaatacacc tacaaattca aataaacggc ccgccgtttg
     1441 attgtagaaa tgccgtctga aacagatttc cgtttcagac ggcattgttt taggcgcgat
     1501 ttatagtgga ttaacaaaaa tcag
//
LOCUS       DAXHRI010000244         1498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXHRI010000244 DAXHRI010000000
VERSION     DAXHRI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.244"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            80..1417
                     /locus_tag="ACFM75_002083"
     CDS             80..1417
                     /locus_tag="ACFM75_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6256544.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          272 a          359 c          402 g          465 t
ORIGIN      
        1 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tactatcttc
       61 acatttctta ataaatttta tgagtaacca tacttcttgg tcgtccaaaa tcggtttcgt
      121 gcttgctgcg gcgggttcgg ccatcggttt gggcgcgatt tggaaattcc cttatacggc
      181 gggcaccaac ggcggcgcgg tgtttttcct gctgtttttg atatttacta tcttggtcgc
      241 cctgcccgtt cagcttgccg aattttatat cgggcgcacg ggcggtaaaa atgccgtcga
      301 ttccttcagg gctctgcgtc cgggtacgca atggctttgg gtcgggcgca tgggcgttgc
      361 cgcctgcttt attttgctgt cgttttacag cgtggtcggc ggatgggtat tgaattatgt
      421 cgtccacagt tttacagggg caatccatgc gggagcggac tttgaagccc tgttcggcac
      481 aacgatttcc aatccggcag gttcgctgtc ctatcaggca ctgtttatgc tgattacggt
      541 ttgggtggtc aaaggcggga tttcagacgg catcgaaaag gcaaaccgtt atctgatgcc
      601 ggggctgttt atcctcttta ttgcgctggc aatccgttcg ctgacgctgc cgggtgcaat
      661 ggagggcgtg tctttcctgc tcaaaccgaa ttggtcgtac tttaaagccg atacgatgat
      721 tacggcttta ggccaggcgt tttttgccct gagcatcggc gtttccgcca tgattaccta
      781 cgcttcatat ttgggaaaag atcaggatat gttccgctcg gggcatacga ttatgtggat
      841 gaacctcttg gtttcgctgt tggcaggttt ggtgattttt ccggcggtgt tcgccttcgg
      901 ttttgaaccg agccaggggc cgggattgat ttttatcgta ttgccggcgg tgtttatgaa
      961 aatgccgttc ggtacggttt tgtttgcggt atttatgctg ctggtcgttt tcgccacgct
     1021 gacttcggca ttttcgatgt tggaaacggt catcgccgca accatccgcc aagacgagcg
     1081 caaacgcaaa aaacacactt ggcttatcgg cacggccatt ttcattgtcg gcatcccatc
     1141 cgcgctgtct ttcggcgtat ggggtgagtt taaggttttc ggcaaaacca tttttgattt
     1201 gtgggactat gttatttccg ccgtcattat gcctgtcggc gcgttgggcg ttgccgtatt
     1261 taccgcctgg attcaggaca agcagtctgt gttaaaagat gccggcgcgg gcagcaccgt
     1321 accacgggca gtgctgctgc tgtggctgaa taccttgcgc taccttgccc cgattgccat
     1381 tattattgtt ttcgtcaatt ctttggacat cctttaaaag ccatccaaac agcaaaaatg
     1441 ccgtctgaaa gcctttcaga cggcattttt gcttcaggtt cagcctattt cgttcaaa
//
LOCUS       DAXHRI010000245         1497 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXHRI010000245 DAXHRI010000000
VERSION     DAXHRI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.245"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..455)
                     /locus_tag="ACFM75_002084"
     CDS             complement(<1..455)
                     /locus_tag="ACFM75_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNme5 family transposase"
                     /protein_id="HFC6256545.1"
                     /translation="MLIHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIEYLKKHK
                     VALVVTESTGGLEIPAAKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFF
                     AQMTAQKEDSQTMPYQPPTEAEEVLEALVNRRNQPADMRTAEKNRLHQV"
     gene            683..1489
                     /locus_tag="ACFM75_002085"
     CDS             683..1489
                     /locus_tag="ACFM75_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6256546.1"
                     /translation="MSSLLFSSLLFSSLLFSSLLFSSLLFSSAAQAASKDNDRSPYYV
                     QADLAYAAERITHNYPEPTGADKDKISTVSDYFRNIRAHSIHPRVSVGYDFGDWRIAA
                     DYASYRKWNSNKYSVNTKEVQENGNTRQVLKTENQENGTFHAVSSLGLSAIYDFKLND
                     KFKPYIGARVAYGHVRHSIDSTKKTTNVVTVAGAANIAPTIYYAPETQNAYHESHSIR
                     RLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYRF"
BASE COUNT          367 a          396 c          354 g          380 t
ORIGIN      
        1 tgaacctgat gcagacggtt tttctcggca gtccgcatat ccgccggttg gttgcggcgg
       61 ttaaccaagg cttccaacac ttcttccgct tcggtgggcg gttggtaggg catggtttgc
      121 gaatcttctt tctgtgccgt catctgtgcg aagaaggcgg gcattttggc atctttggcg
      181 tcggttttgg tcagcggctg cgattgggca aactgatgcg tctgacgcgg gttggcgata
      241 atcacggcta tgcctgctcg gcggatggct ttggcggcgg ggatttcgag accgccagta
      301 ctttccgtca cgacgagggc gaccttgtgt tttttaaggt attcgatagt atgggcgata
      361 cctttggggt tgttggtttc ggttttggtt ttagacaaag acgaaacggc gatgacgaag
      421 tttcgtttgg cgatgtcgat atagtgaatt aacaaaaatc aggacaaggc ggcgagccgc
      481 agacagtacg gatagtacgg aaccgactca cttggtgctt cagcacctta gagaatcgtt
      541 ctcttttttg ttcatccgct atattgtgtt gaaacatcgc cccaaacccg atacaatcca
      601 cccctacaac atcagtgaaa atctttcttt ttaatcagtt aaaaccgaat acggagtcga
      661 aaatgaatcc agcccccaaa aaatctcttc tcttctcttc tcttctcttc tcttctcttc
      721 tcttctcttc tcttctcttc tcttctcttc tcttctcttc tcttccgcag cgcaggcggc
      781 aagtaaagac aatgatcgca gcccgtatta tgtgcaggcg gatttagcct acgccgccga
      841 acgcattacc cacaattatc cggaaccaac cggtgcagac aaagacaaaa taagcacagt
      901 aagcgactat ttcagaaaca tccgtgcgca ttccatccac ccccgggtgt cggtcggcta
      961 cgatttcggc gactggagga tagcggcaga ttatgccagt tacagaaaat ggaacagcaa
     1021 taaatattcc gtaaacacaa aagaggtgca agaaaacggt aacaccaggc aagtcctgaa
     1081 gacggaaaat caggaaaacg gtacattcca cgccgtctca tcgctcggtt tgtccgccat
     1141 ttacgatttc aaactcaacg ataaattcaa accctatatc ggtgcgcgcg tcgcctacgg
     1201 acacgtcaga cacagcatcg attcgaccaa aaaaacaaca aatgttgtta ccgtcgccgg
     1261 tgctgctaac atagcaccta cgatttatta tgcaccagag acgcaaaacg cctatcacga
     1321 aagccacagc atccgccgct tgggtcttgg tgtcatcgcc ggtgtcggtt tcgacatcac
     1381 gcccaagctg accttggaca ctggataccg ttaccacaac tggggacgct tggaaaacac
     1441 ccgcttcaaa acccacgaag tctcattggg cgtgcgctac cgcttctgat tccccga
//
LOCUS       DAXHRI010000246         1459 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXHRI010000246 DAXHRI010000000
VERSION     DAXHRI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.246"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(9..1354)
                     /locus_tag="ACFM75_002086"
                     /pseudo
     CDS             complement(9..1354)
                     /locus_tag="ACFM75_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
BASE COUNT          345 a          344 c          339 g          431 t
ORIGIN      
        1 cagacacttt aagcccatga aatcggtagt cttgcgaatt tgtcgtaaat gaagttgtta
       61 tagctttctt cattgttgac gtgtttttgc tgttcaagct gtttttcaag attctcgtaa
      121 tattcgtaca tatagtaagg gtcattgtac ggtttgaatg cgggctgttc atgaatggct
      181 tgagctttca aaaaggcgca gtcgtaggct tcgggagcca aagacttggg cagcttgtga
      241 tgactcggct caatcagttc aaacagtttg gctttgtcca attcgggaaa aatgaatttc
      301 agaccgtttg ccgcacgtcc gaactgtttt tttacccatt caaggtagcg gtcggctgaa
      361 atgaccttat cttccttaac cgcgtgtatg cgcgttgcct tttgggcgaa tcgttcgcaa
      421 atcggatatg cgccgccgaa atattcgccc ggattctgca aaacttcgaa agggataacg
      481 atgtcttttg ctttgaattc aatttcaaag cgcgtccatg tgcttgtttt atcgcccaac
      541 tgcttgcctt tttcatagac gcggacatat ttggacgatt cacgggagcc gataccatag
      601 gtcttgcctt tggtcatttt ggcttcatcg tcttcttccc aatctgaccc caaacattcg
      661 ccttttggtt tgacgtgatg acaggtaaac atacctttat ttcggtcttc acgggcttgg
      721 ttcgggctgt attcgccgtt gaaaaagtct tttgcgatgt caacgcgtgt gatttttggg
      781 cggattgcat tagtcaggaa tgtgaaaagt cgtgattccc agccttcttt tgcgacgccg
      841 caaccggtgc cggtcagttc gaaaaggatg gtattgtttt gtccgccaaa atggacgcga
      901 ccgtatagga cgtcttccga acccatcaac caacagcgct cataaaaacg accgcccgag
      961 cctttggact ctttgtagat accgaaaccg aaaacttctt cggcgagcat ggacgcggcg
     1021 cgaataaaat cttcgtcttc caaaagactt acacgaacgc cgtatttatc gaaaaaggtt
     1081 ttttcatgaa atgaaaagct aatttgatca atgaaagccg aatctgatac accgcgccga
     1141 agaggaacgc ctaacaggtt tcctttaccg tccgttatgt acgtttcgta acattcgaag
     1201 acttcctgaa ccctgcctgc cgtttcggtt tctgtacccc ccccctgtta gataaggggg
     1261 gaaggtttga agcggttgcc ggcttcctgc cgtccgtcgg cgcgtccgtc atcacgccgg
     1321 caaccgcctt tgtcatccct tgcttatctt ccatggtgcg aatcctcaaa aacgggcaaa
     1381 aaaaagccct gttacttgta gaaagtaaag gacgttaatt tttgttaatc gtcccttctt
     1441 agggacgcaa tatataagg
//
LOCUS       DAXHRI010000247         1411 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXHRI010000247 DAXHRI010000000
VERSION     DAXHRI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.247"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            54..623
                     /gene="rsmD"
                     /locus_tag="ACFM75_002087"
     CDS             54..623
                     /gene="rsmD"
                     /locus_tag="ACFM75_002087"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC6256547.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDSIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGRL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            746..997
                     /locus_tag="ACFM75_002088"
     CDS             746..997
                     /locus_tag="ACFM75_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC6256548.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            1094..1177
                     /locus_tag="ACFM75_002089"
     tRNA            1094..1177
                     /locus_tag="ACFM75_002089"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1128..1130,aa:Tyr,seq:gta)
     gene            1208..1281
                     /locus_tag="ACFM75_002090"
     tRNA            1208..1281
                     /locus_tag="ACFM75_002090"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1240..1242,aa:Gly,seq:tcc)
     gene            1291..1365
                     /locus_tag="ACFM75_002091"
     tRNA            1291..1365
                     /locus_tag="ACFM75_002091"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1323..1325,aa:Thr,seq:ggt)
BASE COUNT          382 a          329 c          356 g          344 t
ORIGIN      
        1 atattctttg acaggtcttg tataataccg tttgaactta caggtttttg attatggcgg
       61 caggcaaaca taccaaacac agcaaccggg tacgcattat cggcgggcaa tgccggggca
      121 ggaaattgag tttcacatcc gccgacggac tgcgtccgac acccgacagc gtgcgtgaaa
      181 agctgtttaa ctggctggga caggatttga cgggtaaaac ggttttggat ctcttcggag
      241 gcagcggcgc actcggtata gaagccgctt cgcgcaacgc caaacgcgtg ctgatttcgg
      301 ataacaaccg ccaaaccgtg cagaccttgc agaaaaacag tcgcgaactg ggtttggggc
      361 aggtgcaaat cgtctgttca gacagcatcg catatttgaa gaccgtatcc gaacagtttg
      421 atgtcgtctt tctcgacccg ccgtttgcat ggcaggactg gcaaatcctg ttcgatgcct
      481 tgaagccgtg cctgaacccc cgggcattcg tctatctcga ggcgggtagg ctgccggata
      541 tccccgattg gctgacggaa tatagagagg ggaaatcggg gcagagtaca tttgaattaa
      601 gggttttcca agtggctgaa taatatgcgc tttgatgatc atttccgagt tgtaaacatt
      661 cgtttgcaac cgtccggttc aaaaaaacct tgtgctataa tccgcgcccg cccggttttg
      721 ataatttagt ggaaaaggaa aagaaatgtc gctttttatt accgacgagt gcatcaactg
      781 cgacgtatgc gaacccgaat gccccaatga tgccatctcg caaggcgagg aaatttacga
      841 aatcaacccc aacctctgca cgcagtgcgt cggacactac gatgaaccgc agtgccagca
      901 ggtttgcccg gtggactgca tcctgattga cgaagaacat cccgaaaccc atgacgagtt
      961 gatggcgaaa tacgaaaaga ttatccagtt taaataaatt ctttttaaaa catcaaatta
     1021 tgtctgtttt gaaataaaat caaaaaaaaa cttgacggaa aagcaagccg ctaataaact
     1081 aacgttctct tttggaggga ttcccgagcg gtcaaagggg gcagactgta aatctgttgc
     1141 gaaagcttcg aaggttcgaa tccttctccc tccaccaaaa ttcttacttg gggcagtagc
     1201 gagtaatgcg ggtgtagctc aatggtagag cagaagcctt ccaagcttac ggtgagggtt
     1261 cgattccctt cacccgctcc aaacaattag gcccatgtag ctcaggggta gagcactccc
     1321 ttggtaaggg agaggtcggc agttcaaatc tgcccatggg caccatatct cgattattca
     1381 tttctttaag gcttagatat ataggatatt g
//
LOCUS       DAXHRI010000248         1405 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXHRI010000248 DAXHRI010000000
VERSION     DAXHRI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.248"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            4..234
                     /locus_tag="ACFM75_002092"
     CDS             4..234
                     /locus_tag="ACFM75_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6256549.1"
                     /translation="MAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNIWKIYFSDESV
                     IFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
     gene            176..616
                     /locus_tag="ACFM75_002093"
     CDS             176..616
                     /locus_tag="ACFM75_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6256550.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLERSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLAHDVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            1214..>1405
                     /locus_tag="ACFM75_002094"
     CDS             1214..>1405
                     /locus_tag="ACFM75_002094"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6256551.1"
                     /translation="MTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKK
                     YIDGVLTAGHGKLYQSDGRH"
BASE COUNT          422 a          297 c          325 g          361 t
ORIGIN      
        1 ttaattgctc agaaagaatt tgaagaattt gttaataacg taaaagagta cgatatttta
       61 gagagtttct cttatttctt aaataagaga gtcgcacata atatttggaa gatttatttt
      121 tctgatgaat ctgttatttt tataagaaaa tcagaagaga acggaaaaac tgttcatgaa
      181 ttcgtttatc aagagtacac cgatagttct gattttaatt ctatgttcga gtaattctta
      241 ttctgaacct gtaagattag aaagatcaca aattaaattt caatcaagta ataatttaaa
      301 atcaagtggt tttaaattag attcaagttc taagagtttt gccaaattca tagaagcggc
      361 aaattttgag cacatcccta cgggcgcaaa agcccgaatc aacgccaaaa taaccgccag
      421 cgtttcccgc gccggcgtat tggcgggggt cggcaaactt gcccgcttag gcgcgaaatt
      481 cagcacaagg gcggttccct atgtcggaac agccctttta gcccacgacg tatacgaaac
      541 tttcaaagaa gacatacagg cacgaggcta ccaatacgac cccgaaaccg acaaatttgc
      601 aaaggtctca ggctaagtgc gcctgttgcc gcctaaaagg taacccggat gcctgattat
      661 cgggtatccg gggaggatta agggggtatt tgggtaaaat taggaggtgt tatggagcga
      721 aaacagccga aaacctgtgt ttgggtttcg gctgtcggga gggaaaggaa ttttgcaaag
      781 gtctcttttc gtcattcccg ccacttttcg tcattcccgc gaaagcggga atctagaata
      841 tcggactttc agataatctt tgaatattgc tgttgttcta aggtctagat tcccgcctgc
      901 gcgggaatga caactcatta gttacctaaa acttaaaaag cagaaacctt tacgccgtca
      961 ttcccacgaa agtgggaatc cgggaactta acgttacagt gatttatcgg aaacggctga
     1021 aaccgaatga attggattcc cgcctgcgcg ggaatgacga atccatccgt acggaaacct
     1081 gcaccacgtc attcccacga acctacattc cgtcattccc acgaaagtgg gaatccagga
     1141 cgaaaaatct ccagaaaccg ttttacccga taagtttccg caccgacagg tctggattcc
     1201 cgcctgcgcg ggaatgacga tgcaggtatt tctgacgatt cccggctatg atgttgaggc
     1261 agaaatcgaa aaattcgttt ggatggatgc tgtgatttgg cagatgccgg gctggtggat
     1321 gcacgagcct tggacagtga aaaaatacat agacggagta ttaaccgctg gacacggcaa
     1381 actctaccaa agcgacggca gacac
//
LOCUS       DAXHRI010000249         1399 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXHRI010000249 DAXHRI010000000
VERSION     DAXHRI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.249"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(39..725)
                     /locus_tag="ACFM75_002095"
     CDS             complement(39..725)
                     /locus_tag="ACFM75_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC6256552.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARQIIFWVMFPALLLSYIFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(801..1274)
                     /gene="queF"
                     /locus_tag="ACFM75_002096"
     CDS             complement(801..1274)
                     /gene="queF"
                     /locus_tag="ACFM75_002096"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC6256553.1"
                     /translation="MSRNTEELQGISLLGNQKTQYPAEYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDSQ"
     regulatory      complement(1278..1322)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT          345 a          291 c          435 g          328 t
ORIGIN      
        1 aagaaaaccc aaccgtcccg attcccggca gggctgtttt acggattttg cagcgagggc
       61 gcggggcggt cttgcgcctg tttggtttgc agggtcgtca gttttttcgt cagcagattc
      121 agaatcacgc cgtaggcggg caggaaaaac agaccgcaga cggtgagttt gaacaggtaa
      181 tcgacaaaag cgatgccctg ccagtttgcc gccataaatt catcgctgct tgcatagaag
      241 gcaacggcga aaaataccaa cgtatccaag gcgttgccga tgacggttga tgcggttggg
      301 gcaatccacc acgctttcag acggcgtaat ttgttgaata caaaaatatc aaggatttgt
      361 ccgagcgcgt aggcggcaaa actggctaag gcgatgcgtc cgacaaaggt attgaattcg
      421 gacagtgcgc ccaagcctgt ccaactgccg ttgtggaaca aaacggaaaa gatgtaggaa
      481 agcaaaaggg cggggaacat gacccaaaag ataatctgcc gtgccaagtg cgaaccgaaa
      541 atgcggacgg tcaggtcggt ggcgaggaag atgaagggaa aggaaaacgc gccccaagtg
      601 gtgtggatgc cgaaaatttg gaaggggaac tgcaccagat agttgctggc ggcgatgatg
      661 aggatatgaa aaagcaccag ccggaagagt gccttctgtt gctgtgcgtc ggtaaatgcg
      721 tacataaaaa aacctttcgg aaaggcgttc agacggcata ccgtatcgaa agaatgccgt
      781 ctgaaagtgg gaaggacgga ttattggctg tcgtgttcga acaagcgttt acgcgccaat
      841 gcttcaaact cggtgcctgc tttgccgtag ttggcaaacg gatgaatggc gatgccgccg
      901 cgcggtgtga actcgccgaa tacttcgatg tatttcggat ccatcagggc aatgaggtct
      961 ttcatgatga tgttgacgca gtcttcatga aaatcgccgt ggttgcggaa gctgaagagg
     1021 tagagtttca gggatttgct ttccaccatt ttgatgtgcg gaatgtagcg gatgtagatg
     1081 gtggcgaagt cgggctgccc ggtcatgggg cagaggctgg tgaactctgg gcagacgaat
     1141 ttgacgaaat agtcgttgtc gggatgtttg ttgtcgaacg cttccaaaat ttcgggcgcg
     1201 tattcggccg ggtattgggt tttctgatta cccaaaagcg agatgccttg cagctcttcg
     1261 gtattgcggg acatagggtt tccttagttt tttagtgtgg gaggttttcg aaccacgggg
     1321 cggcgattgt aatataagcg gcggtatctg tgtagttttc ttcagacggc atggtttgga
     1381 cggcggcgtt ttccgtgtc
//
LOCUS       DAXHRI010000250         1372 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXHRI010000250 DAXHRI010000000
VERSION     DAXHRI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.250"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            4..276
                     /locus_tag="ACFM75_002097"
     CDS             4..276
                     /locus_tag="ACFM75_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256554.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            589..1167
                     /locus_tag="ACFM75_002098"
     CDS             589..1167
                     /locus_tag="ACFM75_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFC6256555.1"
                     /translation="MSPTHEISNVERLIDEINRIHAGYHERVSKLYGGNLPNLSKTLR
                     HLDIDFVLQYRLNLHESINDYLIRSDIGDLPLAYYYRVKTTESVFDKIRWFSERESGY
                     PANKVLNDIFGVRIIADSALMEEIHAALDEWVSTKGLKNWNVQEKNGYIGTHVYFNQK
                     NNLFFPWELQIWDKADALKNIESHERDKRLFV"
BASE COUNT          418 a          228 c          300 g          426 t
ORIGIN      
        1 atcatgaata agagaatgaa aatgtgtcct gcttgtcaac aaggctatct ctaccattcg
       61 aaacctaaat atcttcatga tgaaattatt ctgtgtgatg aatgcgatgc agtatggctc
      121 aaaggtatga atatatttta tggagaatat gaaaaagatt tttattctta tgttcctttc
      181 atggaatccc aaggtataac gagtgaatgt atttgggaag gagatttgtt tgatcatcca
      241 tattatgaag atgaaaactc aaatgatatg gattgatgga aattttaagc ctgcgtaggt
      301 acgattagcc atcaaacggc gctgtcttta atgcaatgtc gttttcagta cgggaggctg
      361 cttggaggcc gagtagtcgc tcctttgtct tcatgggatg gattggattc attgatgacc
      421 tgtaatctgt taccgtgtat gttttttgga tttgcaattc ctcctatttt agcgtattaa
      481 cggtaatgaa aaattaaaag gaagttttga aatttgtttt gtataatata tagttgaaag
      541 caattagtat gttattttca tataattgga atgaaagagt aacaaggaat gtcccccact
      601 catgaaatca gtaatgtaga acggctgatt gacgaaatta accgcataca cgccggatat
      661 cacgaaaggg tttccaaact gtatggcgga aaccttccca atttgtcaaa gacgctccgg
      721 catctggaca tcgattttgt gttgcaatac cgacttaatt tgcatgagtc aatcaacgac
      781 tatctgattc gatctgacat cggggacttg cctttggcat actactaccg cgttaaaacc
      841 acggagtccg ttttcgacaa aatccgctgg ttttcggaac gcgagtccgg gtatccagcg
      901 aataaggtgt taaatgatat ttttggggtg agaatcatcg cagattccgc actaatggaa
      961 gaaatacatg ctgctttaga tgaatgggtt tcgacgaagg gcttaaaaaa ctggaacgtt
     1021 caggaaaaaa acggctatat cggaacgcat gtgtatttta atcaaaaaaa taatctgttt
     1081 tttccgtggg aacttcagat atgggacaag gcggatgcct tgaaaaatat tgagagccat
     1141 gaacgggaca agcgactttt tgtttaacct ttaatgggtc tttttaatat agcaccgttt
     1201 accgtgcata ccttgggtgt acagatttaa ttattaaagg aactctcgtg tttcccgaca
     1261 aatacaagtt gagtttgaaa gaaaatattt ttttggcaaa gaaagtattg gttgcccaaa
     1321 ttcacaacct cagccgtttt gagaattgtc agacgacctt attgcagact ga
//
LOCUS       DAXHRI010000251         1356 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXHRI010000251 DAXHRI010000000
VERSION     DAXHRI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.251"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>1356
                     /locus_tag="ACFM75_002099"
     CDS             <1..>1356
                     /locus_tag="ACFM75_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6256556.1"
                     /translation="EIEKLAKQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTG
                     AGAAIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALA
                     SLYSQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVR
                     TELFSSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQ
                     GEAASKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRN
                     PATLSDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKK
                     HQPQKPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVY
                     AAGWTAPLIGTAGKAAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGA
                     LS"
BASE COUNT          385 a          325 c          372 g          274 t
ORIGIN      
        1 cgaaatcgaa aagctggcca aacagcccga atacgcctac ctgaaacagc ttcagacggc
       61 caagaacgtc gattggaaac aggtgcagct ggcctacgac aaatgggact ataaacagga
      121 aggcttgacc ggagccggtg cagcgattgt ggtgattatt gtaaccgctc taacttatgg
      181 atacggagcg gctgcagcgg gtagcgtaac tgccgcagga agtagtacag ccgcagctgc
      241 aacaacgaca gcggcagcaa ctaccactgt ttctactgca gctgccatgc aaaccgcagc
      301 tttagcctcc ttgtatagcc aagcagctgt agccatcatc aataataaag gcgatgtagg
      361 caaagcatta aaagatctcg gcaccagtga tacggtcaag cagattgtta cttccgcact
      421 gacggcgggt gcattaaacc agatgggagc agatattgcc caattgaaca gcaaggtaag
      481 aaccgaactg ttcagcagta cgggcaatca aaccattgcc aaccttggag gcagattggc
      541 caccaacctc agcaatgcag gtatctcagc tggtatcaat accgccgtta acggcggcag
      601 cttgaaagac aacttaggca atgccgcatt gggagcattg gttaatagct tccaagggga
      661 agccgccagc aaaatcaaaa caaccttcag cgacgattat gttgccaaac agttcgccca
      721 cgctttggcg ggttgtgtta gcggactggt acagggaaaa tgtaaagatg gggcaattgg
      781 cgcagcagtt ggggaaatcg tagctgaatc catacttggc ggcagaaacc ctgctacact
      841 cagcgatgca gaaaagcata aagttatcag ttactcgaag attattgcgg gcagcgtggc
      901 ggcactcaac ggcggcgatg tgaatactgc ggcgaatgcg gctgaggtag ctgtggtgaa
      961 taatgctttg aattttgaca gcacccctac caatgcgaaa aagcatcaac cgcagaagcc
     1021 cgacaaaacc gcactggaaa aaatcatcca aagtattatg cctgcacatg cggcaggtgc
     1081 gatgactaat ccgcaggata aggatgctgc catttggata agcaatatcc gtaatggcat
     1141 cacaggcccg attgtgatta ccagctatgg agtttatgca gcaggttgga cagctccgct
     1201 gatcggtaca gctggtaaag cagctatcag cacctgtatg gctaatcctt ctggttgtac
     1261 tgtcatggtt acgcaggctg ctgaagcggg cgcgggaatc gccacgggtg cggtaacggt
     1321 aggcaacgct tgggaagctc cagtaggagc gttgtc
//
LOCUS       DAXHRI010000252         1355 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXHRI010000252 DAXHRI010000000
VERSION     DAXHRI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.252"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..380)
                     /locus_tag="ACFM75_002100"
     CDS             complement(<1..380)
                     /locus_tag="ACFM75_002100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256557.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="ACFM75_002101"
     CDS             complement(438..803)
                     /locus_tag="ACFM75_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256558.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1333)
                     /locus_tag="ACFM75_002102"
     CDS             complement(785..1333)
                     /locus_tag="ACFM75_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256559.1"
                     /translation="MKKQITAAMMILTMIAAPAMANSMDNQIFENQVFHTRADAPMQL
                     AELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIP
                     GGVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPG
                     QGIGRHRPWESKSTDRSWKNRF"
BASE COUNT          415 a          315 c          204 g          421 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcaccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccccctcag tctccttcat ctccttctga gaaagctccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccatcgcc ggagcggcaa tcattgtcag aatcatcatc gctgcggtaa
     1321 tttgtttttt cataataact cctttggatt acaag
//
LOCUS       DAXHRI010000253         1318 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXHRI010000253 DAXHRI010000000
VERSION     DAXHRI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.253"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(39..962)
                     /gene="lpxC"
                     /locus_tag="ACFM75_002103"
     CDS             complement(39..962)
                     /gene="lpxC"
                     /locus_tag="ACFM75_002103"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC6256560.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPAFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          346 a          325 c          330 g          317 t
ORIGIN      
        1 tttaagtttt aggaatttac cggaaaaaac agaaagcgtt atccacaagt tctgatgttc
       61 agctcgtgaa atgcgtcggg caaatcatcg ctgtcggcaa attccacccg gtcgtaagcc
      121 gtttcgtctg ccaaaaccgc gcgcaagagt gcgttgttga tggcgtgtcc tgatttgtag
      181 ccttcaaacg cgccgacaat cggatgtccg acgatataca aatcaccgat ggcatcaagg
      241 attttgtggc gcacaaactc atcgggatag cgcaagcctt caggattcag gacatccgtg
      301 tcgtcaatca cgatggcgtt gttcaaattg ccgcccaaac ccagattgtg ggcgcgcatc
      361 atttccactt cgtgcataaa gccgaaagtg cgagcgcgcg cgatttcgtc gatgtaggat
      421 ttgccggcga aatcgatttc aaaagtgggc gagctgcggt tgaaagccgg atggtcgaat
      481 tcgatggtca gcgttacctt aaagccgtca tacggcgtaa agcgcaccca tttgcccgct
      541 tctttgattt cgacaggctt gaggattttc aaaaaacgct tttgcgcctt ttgatcgacc
      601 acgcccgcat cttgcaaaag gtaaataaac ggcaggctgg agccgtccat aatcgggatt
      661 tcgggtgcgt tcagctcaat cagcgcgttg tcgataccgt aggcggacag cgcggacata
      721 atgtgttcga tcgtgccgac gcgcacgcct ttgtcggtaa cgatggtgga ggaaaggcgg
      781 gtatcgttga tcaaataagg ggtcagcttg atttgttcgc ccatctcgcc gtccaaatcg
      841 gtacggcgga aggaaatccc gctgttttca ggcgcggggt gcagggtcag tgcgacccgt
      901 tcgcccgaat gcagcccgac tccggtaacg ctgatggatt tcgccaaagt tctttgcagc
      961 ataaaccgct tccttatcaa tggggtaagt tttggaataa tacgataaaa ccggaaaaac
     1021 aggctatgtt tttccatagt atttgccaat gtatccgttt tcaatacgta agccgcataa
     1081 aaatgtatag tggattaaca aaaatcaaga caaggcgacg aacccgcccc ctcctgaaaa
     1141 acgcaaaaaa tgccgtccga aaacctttcg gacggcattt tcgcgtgaac cgtcattccc
     1201 acaaggacat aaaaccaaaa cagaaaaccc aaaacagcaa cctaaaattc gtcattccca
     1261 cgaaagtggg aatccagttc gttcggtttc gcttgtttta agtttcgggt aacttcca
//
LOCUS       DAXHRI010000254         1289 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXHRI010000254 DAXHRI010000000
VERSION     DAXHRI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.254"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            166..1278
                     /gene="nadA"
                     /locus_tag="ACFM75_002104"
     CDS             166..1278
                     /gene="nadA"
                     /locus_tag="ACFM75_002104"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC6256561.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSHPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
BASE COUNT          308 a          343 c          377 g          261 t
ORIGIN      
        1 accaaagcgg tttgtcggaa cgaagcgggt cggttttacg gtatcgcaaa cctgttttca
       61 gacgaccttg cttattatac tcaatttgag cataatataa aaatatttca aaatataaac
      121 ggcaaatctt tatgccgtct gaaaaaccag ccataaggaa gtctcatgca aaccgccgcc
      181 cgccgctcgt tcgattacga tatgcccctc atccaaaccc cgacttccgc ctgccaaatc
      241 cgtcaggcgt gggcgaaggt tgccgacacg cccgaccgcg agacggcagg ccgtctgaaa
      301 gacgaaatca aggctttgct gaaggagaaa aacgcggtct tggtggcgca ttattacgtc
      361 gatccgctga ttcaggattt ggctttagaa acgggcggat gcgtgggcga ttcgctggaa
      421 atggcgcgct tcggcgcgga acacgaagct agtacattgg tggtggcggg tgtgcgcttc
      481 atgggcgaga gcgcgaaaat cctctgccct gaaaaaacag tgctgatgcc tgatttggag
      541 gcggaatgtt ctttggattt gggctgtccg gaagaagcgt tttcagcgtt ttgcgaccaa
      601 caccccgacc gcacggtcgt agtgtacgcc aacacttccg ccgccgtgaa agcgcgtgcc
      661 gattgggtgg taacgtcttc ggtggcgttg gaaatcgtat cgtatttgaa atcgcgcggc
      721 gagaagctga tttggggacc tgaccgccac ctcggcgact acatccgccg cgaaacgggc
      781 gcggatatgc tgctgtggca gggttcgtgc atcgtccaca acgaattcaa agggcaggag
      841 ctggcggcgt tgaaggcgga acaccccgaa gcggtggtgc tggttcatcc cgaatcgcca
      901 caaagcgtca tcgaactggg cgacgtggtc ggctcgacca gcaaactgct caaagccgcc
      961 gtatcgcacc ctgaaaaaaa attcatcgta gcgaccgact tgggcatcct gcacgaaatg
     1021 caaaagcagg cacccgacaa agaatttatc gccgcaccga cggcgggcaa cggtggaagc
     1081 tgtaaaagct gcgcgttctg cccttggatg gcgatgaatt cgctgggcgg catcaaatac
     1141 gccctgacaa gcggacacaa cgaaatcctg ttggacagga agctgggcga agccgccaaa
     1201 ctgcctttgc agcgtatgct cgacttcgcg gcaggactca agcgcgggga tgtgttcaac
     1261 ggcatgggac ccgcctgatt tgccgtcca
//
LOCUS       DAXHRI010000255         1261 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXHRI010000255 DAXHRI010000000
VERSION     DAXHRI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.255"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..365)
                     /locus_tag="ACFM75_002105"
     CDS             complement(<1..365)
                     /locus_tag="ACFM75_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6256562.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQIL"
     gene            complement(513..665)
                     /locus_tag="ACFM75_002106"
                     /pseudo
     CDS             complement(513..665)
                     /locus_tag="ACFM75_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
     gene            complement(949..>1261)
                     /locus_tag="ACFM75_002107"
     CDS             complement(949..>1261)
                     /locus_tag="ACFM75_002107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256563.1"
                     /translation="VHEMTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNT
                     FSNPPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGL
                     P"
BASE COUNT          383 a          266 c          246 g          366 t
ORIGIN      
        1 atcagtatct gttgcgcggc tttttgtagc ctgctcaaat tattggaacc taaacacatc
       61 ccagttttaa aagctgtttc tttcatgagc acagaaggaa gaaaagaaaa tttgcgcgcc
      121 gtcttatcat ctaaactaat gtaatttacc cgagtacacg gtgtttcatc ttcactcagg
      181 acataattgt tcttctccaa tggttgctcg aaacggacat ttgcatcagt taacaattca
      241 gcatctatgt gctgctgacg ctgcatggaa cggataagtt ctgcatcgtt ttcatcggca
      301 gctaaggttt taaggggtat gacagccagg ataaccaaca gacatggagc aggaaaaaat
      361 ttcatgacat caatattatt ttagcaatat ttactatttt gtcataaatt taaaagtatt
      421 tacagttata gaatgagacc tttgcaaaat tccccaaaat tcccaccaag acatttaggg
      481 gattttgggg aattttgcaa aggtctcgga cagtattttg aacgcagtgc gcgtaaatcc
      541 gtatggaaac catgaaatcc cgccacagcc gccagacatg ccaagccgca ttctgatatt
      601 tctgtttgca ggataacagg cagctttttc tttaagccca aagacaggtt ttgcagatgg
      661 ggcatagatt tcctttttga aaaataggga ttaggaagtt ggatgtattt tagaaaggcc
      721 gcctgaaaag gtttcagacg accttttgcg actagctgct attttattta aagcttttct
      781 ctaacaaacg agctaatatt tttcctgtaa taaaacagat aaaaaacagc atccaatacg
      841 tcagattgga aaaatcggtc gtatagagaa tcaacatata aagaagcagc atgatgccga
      901 gtgcgatgaa ttgataatgt ttggcaaaca tcatgacctc ctcaactatt aaggcaaacc
      961 gcctgaatat tctcgttcaa tcgtttcggc aatttcccta taacgtcgat accatgacca
     1021 gtcgaaattt tcaatggcat ggctcgcaaa cgtaccaaat tcaggcatcc ctatgcggct
     1081 acctgctaaa gctccgattg tagctcccca accaggcgga ttactgaacg tattgtttgg
     1141 caatcctaaa gatttatcag gatttcccgt atcgttaagc cctgcagatt cgcaaatttc
     1201 gcaataatat gagctttgtt gcgcattacc tgagcctccg acccaagtca tttcatgaac
     1261 a
//
LOCUS       DAXHRI010000256         1257 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXHRI010000256 DAXHRI010000000
VERSION     DAXHRI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.256"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            254..1207
                     /locus_tag="ACFM75_002108"
     CDS             254..1207
                     /locus_tag="ACFM75_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6256564.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          205 a          272 c          427 g          353 t
ORIGIN      
        1 ttgttaatcc actataacaa accggatgat tcgggctaaa taattcctga ttctttggat
       61 atagccaacg gtatgcctgc cgatgccgtc agaaagggct tgatgggcgt ttgcggttcg
      121 ggttaatatt tgtgctgtat ctgttaattc cggttagtaa aatggggcgg ggacgatgag
      181 tggccgtcgg tcggttttgc acgggcggta ataatgtccg gcgcaaggtg cgccgtttcg
      241 ggaggggtgt gtgatgggtt ttctgattgg tttgagccgc aacctgacgc ggtttacggc
      301 tttggtcatt ttgcttgcgt cggcggttgc gtttatcgga ccggcttcgt ttgcgtgggt
      361 taagggcgat gttcaggtct tggtgctggg catcattatg ctgggtatgg ggatgacttt
      421 gggcagggag gactaccgga ttttggcgca gcgtcctttg gatgtgttta tcggtgcggc
      481 ggcgcaatat accgtgatgc cgttgctcgc catcggtatt gccaagctgt tcgacctttc
      541 gccggggctg actttggggc tggtgttggt ggggacgtgt ccgggcggcg tgtcgtccaa
      601 cattatgagt ttccttgcca aaggcgatgt ggcgttttca gtcggtatga cgacggtgtc
      661 tacggtgctg gctccggccg ttacgccttt gtggatgacc tgcctggtcg gtcagacggt
      721 ggatatggac ggttggggga tgttccggtt tatgctgctg gtcacgcttt tgccggtggt
      781 gctgggttcg gcggcaaata tcctgctgca cggaaaacat tggtttgaaa atgtccgtgc
      841 cgttatgccg ggcgtggcgg tattggcatt tgcctgcatt gtcggcggcg tggcttcggt
      901 tcacggacac cgttttgccg aatcggcgtt ggtaatggtg ttggcgattg cggtacacaa
      961 tatcgccggc tatgtgtcag gctattacgc cgccgcgctg tttggtatgg atacggcaaa
     1021 aaaacgcacc ttggcaatcg aagtcggcgt gcagaatgcc ggtttggcaa caggattgag
     1081 cgcgaaattt ttcccaggca accccgaatc ggcggttgcg gcggcggtcg cctgcgtgtg
     1141 gcattcggtt tcggggacgg tattgggcaa cttgttcgct ctgtgggaca agcgtcggga
     1201 cgcttagtcg ggcgtgtccg aaccgtccca cgatatatag tggattaact ttaaacc
//
LOCUS       DAXHRI010000257         1220 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXHRI010000257 DAXHRI010000000
VERSION     DAXHRI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.257"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..192
                     /locus_tag="ACFM75_002109"
     CDS             <1..192
                     /locus_tag="ACFM75_002109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256565.1"
                     /translation="NSDLPSFNTIWQGTADHLSISDSLVRRWVTKYRLHGESGIKRRK
                     HTTKYSVEYKLEAIRLVTG"
     gene            196..309
                     /locus_tag="ACFM75_002110"
     CDS             196..309
                     /locus_tag="ACFM75_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6256566.1"
                     /translation="MSQKAAADQLNLPDCSILLQWLRLYHLNGINGLKPKP"
     gene            649..885
                     /locus_tag="ACFM75_002111"
                     /pseudo
     CDS             649..885
                     /locus_tag="ACFM75_002111"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(938..>1220)
                     /locus_tag="ACFM75_002112"
     CDS             complement(938..>1220)
                     /locus_tag="ACFM75_002112"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6256567.1"
                     /translation="GLRGYLGRIRRYLGRKQPKTCVWVSAVGRDRNFAKVSIFLSTSS
                     HSNQTKAVHRHTHVGSTAGQTTIRSGGDTTLKGAQLIGIGIQADPQLQP"
BASE COUNT          345 a          283 c          292 g          300 t
ORIGIN      
        1 aattccgact tgccgtcgtt caatactatt tggcagggaa cagcagacca tctttctatt
       61 tccgattcat tggtacgcag atgggtgaca aaatacagat tacacggaga gagtggcatc
      121 aaacgcagaa agcatacgac aaaatattcg gtcgaataca aacttgaggc aatccgcctg
      181 gtgacggggt agggaatgtc ccaaaaagct gccgcagacc aactgaattt gcccgactgc
      241 tccatcttgc tgcaatggtt gcgcctctac catttgaatg gtattaacgg tttaaagccc
      301 aaaccctaaa cccaaaggaa gaaagcccgt gaaaaaacag catccgccgg aaacgaaaaa
      361 gccgactatc tgaaaaccaa ggaagaactg cttgcggaat tggcttgcct taaagcggaa
      421 atggctgccc taaaaaagct cgatgcctta atctatggga agaagtgcgg tagaaagaac
      481 gcaactcgtc gcagggttaa ggcaatgcca tccgttgaac tgctgttggt gattgtcgga
      541 ctgccacgcg gcaccttcta ttaccaattg gttgtccaat cggcagaaga caaatatgtc
      601 gatttgaaac ggcatatcca tgatatttat caaaagcagt tgaaagacaa cggtctggtt
      661 cagagtatgt cccgcaaggg aaactgcttg gacaatgcgg caatggaaag tttcttcgga
      721 acgttgaaat cggaatgttt ccatacgtgc aaatatgatt ccgttaccga attggaagct
      781 gtactgcacg aatatatccg ttactacaac aacgatagaa tcaagttgaa attaaaagga
      841 ctgagccctg ttcagtacag aattcagtcc ctgaaagccg cttgattaaa ctgtccaact
      901 tttgggggtc agttaatatc ggttccacca atagtcgtca gggttgtagt tgggggtctg
      961 cctgtatgcc tatgccgata agctgcgcac ctttgagggt ggtatccccg ccgcttcgga
     1021 tggtggtttg tccggccgta ctgccgacat gggtgtggcg gtggactgct tttgtctgat
     1081 tgctgtggct acttgttgaa agaaagatag agacctttgc aaaattcctg tccctcccga
     1141 cagccgaaac ccaaacacag gttttcggct gttttcgccc caaataccgc ctaattctac
     1201 ccaaatatcc ccttaatcct
//
LOCUS       DAXHRI010000258         1192 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXHRI010000258 DAXHRI010000000
VERSION     DAXHRI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.258"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(4..462)
                     /locus_tag="ACFM75_002113"
     CDS             complement(4..462)
                     /locus_tag="ACFM75_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256568.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
BASE COUNT          429 a          217 c          122 g          424 t
ORIGIN      
        1 cgtttagtct agagtctgta tattacattt atttttaggg tctgctagcc aatttcttgt
       61 tcccttcatt attttatctt ctgaaagaaa attatttttt tccatgctat taatattaat
      121 gatatgattt ttatttaaaa taatttttcc atataaaatt tccggtttgt aatagcaaga
      181 gtctgaagca aatccttgcc cgtcaatata agaaaaataa atgttatcat cctccttata
      241 atattctaaa accaatccgg atattatttt atcaaaattc ccttcatttc catctttcat
      301 gactaaaata taattagcag gccaaccttt atcgtaaaca tattcaaact gatagttccc
      361 gaaactctcg atatccgaac taaaaaagaa gaaaagcaaa ttaatagcca atacaaaaac
      421 aaataagaac aaaaatagca aaattttcaa cttagttaac aatatttacc tctcctttaa
      481 attcaatcct gaaaggtacc ccttaccctg ggctaccaat tatagtttcc atatttctaa
      541 atattgtttt tacattactt ttttctcccc ccaaaggaat gcattttaaa atcatgcttt
      601 tcaggtgcta atcgatactt accattaaca tctttaatca cagatatatt tccatgtata
      661 gcccaacgtg aaaaatctga gtattatata cagttatacc ttactgaaac aaagctagct
      721 gtacatatct gtaccatatt tttactacaa acaaaaaata tttataatta ttaaggataa
      781 gataaaaacc cccaacgcca gattaattct taataatttc aaataaaaaa tatttgagat
      841 tttttctttt ccaaataata tattgaaata tttaaagtta aggtagcttg atttaattag
      901 aaaatcaaaa aaaaccatta attaattttt cttctttaaa tattttatat gatattataa
      961 aattaaataa cattgcgaaa acatatgaaa tcatcaaaat accaaatata tagtgagaat
     1021 gcattattat ttagctcctt cttttggctc tgacaaattg acagtccatt gatatccaat
     1081 gcttgcattt gcaattgctg acgcacctgc ccctgttttc cctgttagta tcaatggttc
     1141 ttttcttgca agatgtgcac ctgcacaacc acctaatact aaaccaccac ct
//
LOCUS       DAXHRI010000259         1189 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXHRI010000259 DAXHRI010000000
VERSION     DAXHRI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.259"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..429
                     /locus_tag="ACFM75_002114"
     CDS             <1..429
                     /locus_tag="ACFM75_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6256569.1"
                     /translation="AIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQ
                     PVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTV
                     DVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            436..945
                     /locus_tag="ACFM75_002115"
     CDS             436..945
                     /locus_tag="ACFM75_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256570.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          405 a          216 c          205 g          363 t
ORIGIN      
        1 gctataccaa cccagacagt taaagaactt gatggcttac tacaagaatc aaaaaatata
       61 ggtgctgtaa atacacgaat taatatagcg aatagtacta ctcgatatac accaatgaga
      121 caaacgggac aaccggtatc tgctggcttt gagcatgttc ttgaggggca cttccatagg
      181 cctattgcga ataaccgttc agtttttacc atctccccaa atgaattgaa ggttatactt
      241 caaagtaata aagtagtttc ttctcccgta tcgatgactc ctgatggcca atatatgcgg
      301 actgtcgatg taggaaaagt tattggtact acttctatta aagaaggtgg acaacccaca
      361 actacaatta aagtatttac agataagtca ggaaatttga ttactacata cccagtaaaa
      421 ggaaactaac taaatatgag taactttgaa aaaaaatata ttttagaatt aaatgatgct
      481 ttaagccatt taaatcataa ctctacctca tttgatttat tgaaagtttt gatttcatgg
      541 ttatcaaacg atattgtcat tgataaattt aaaattttag gttatgactt tagtaaatat
      601 atcgaaatga atcccgatga ctatccggtt gaaaaatcta tattgaatag agaggaaatt
      661 atttatctca aaaacaatat ttatcgtaaa atatcatcag gaaattttaa atttcaatac
      721 tttgtacaat atattagaga tattttagaa tatttattta ttgaacatat tgaaagagtc
      781 tgtccttact gcgaatgggg tgaaatgcaa aaattagaag aacaaaatac gcatgaaacg
      841 gtgtatctct gtactcaatg tggatgtgct ttttataacg ataattcaca atttttatta
      901 aaaacccctt taaccattcc aatgaaacgt gatgaattta aataaacaag ccgtagcctg
      961 catgaaccct aaaatccacg tgtagcgtgt gtgcgccagc acgcatgcgt tccatgattt
     1021 acggctcaat gccgtctgaa aagctcacaa tttttcagac ggcatttgtt atgcaagtaa
     1081 atattcagat tccctgtatg ctgtacagac gcgggagtgt taagcccccc ttgtttgaag
     1141 ctccgcggct cctgccgagc ttcaccgacc ccgttgtgcc caagctctc
//
LOCUS       DAXHRI010000260         1168 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXHRI010000260 DAXHRI010000000
VERSION     DAXHRI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.260"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(231..1085)
                     /locus_tag="ACFM75_002116"
     CDS             complement(231..1085)
                     /locus_tag="ACFM75_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6256571.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          328 a          348 c          308 g          184 t
ORIGIN      
        1 tcccgtcatt cccgcgcagg cgggaatccg gacatccaac gctgcagcaa tttgtcggaa
       61 ataaccgaaa ctcaaaaaac cggatgcccg cctgcgcggg aatgacgacg caaagatggt
      121 cgttatttcg gataaatatc cgtcttttcg ttccggatac ccgctttcgc aggaatggag
      181 atggaaagat ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga
      241 tgcgacagcc ggccggcaac caaggtggcg gcgatgacga caaacacgcc caaggcggac
      301 acgggcgaca ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg
      361 agcgaaatca acagtcccga aacgttggca ggaacacggc tcatcccctt gttccacagc
      421 caataggcgt accagccgca ccccaaaccc aaatacagca gcgacaatac catcccgacg
      481 ctccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
      541 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc
      601 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
      661 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
      721 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
      781 acaaacacca tcagcagtgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
      841 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc
      901 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
      961 gcaggcagcg cggcaattag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
     1021 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
     1081 aacatagtga gtgataaagt cagatggtca tacaaaagta tagcggatga acaaaaatca
     1141 ggacaaggcg gcgagccgca gacagtac
//
LOCUS       DAXHRI010000261         1158 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXHRI010000261 DAXHRI010000000
VERSION     DAXHRI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.261"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..773
                     /gene="mafB"
                     /locus_tag="ACFM75_002117"
     CDS             <1..773
                     /gene="mafB"
                     /locus_tag="ACFM75_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6256572.1"
                     /translation="RGIVQGAVNPFLMGFQGVGIGAITDSAVSPVTDTAAQQTLQGIN
                     DLGKLSPEAQLAAASLLQDSAFAVKDGINSARQWADAHPNITATAQTALAVAEAATTV
                     WGGKKVELNPTKWDWVKNTGYKTPAVRTMHTLDGEMAGGNRPPKSITSNSKADASTQP
                     SLQAQLIGEQISSGHAYNKHVIRQQEFTDLNINSPADFARHIENIVSHPTNMKELPRG
                     RTAYWDDKTGTIVIRDKNSDDGGTAFRPTSGKKYYDDL"
     gene            783..989
                     /locus_tag="ACFM75_002118"
     CDS             783..989
                     /locus_tag="ACFM75_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256573.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
BASE COUNT          396 a          216 c          254 g          292 t
ORIGIN      
        1 ttcgcggcat cgtccaaggt gcggttaatc cttttttaat gggttttcaa ggagtaggga
       61 ttggggcaat tacagacagt gcggtaagcc ctgtcacaga tacagccgct cagcagactc
      121 tacaaggtat taatgattta ggaaaattaa gtccggaagc acaacttgct gccgcgagcc
      181 tattacagga cagtgccttt gcggtaaaag acggcattaa ttccgccaga caatgggctg
      241 atgcccatcc gaatataaca gcaacagccc aaactgccct tgccgtagca gaggccgcaa
      301 ctacggtttg gggcggtaaa aaagtagaac ttaacccgac caaatgggat tgggttaaaa
      361 ataccggcta taaaacacct gctgttcgca ccatgcatac tttggatggg gaaatggccg
      421 gtgggaatag accgcctaaa tctataacgt ccaacagcaa agcagatgct tccacacaac
      481 cgtctttaca agcgcaacta attggagaac aaattagtag tgggcatgct tataacaagc
      541 atgtcataag acaacaagaa tttacggatt taaatatcaa ttcaccagca gattttgctc
      601 ggcatattga aaatattgtt agccatccaa caaatatgaa agagttacct cgcggtagaa
      661 ctgcgtattg ggatgataaa acagggacaa tagttatccg agataaaaat tctgacgatg
      721 gaggtacagc atttagacca acatcaggta aaaaatatta tgatgattta taggaaaaag
      781 ccatgaatat actatccata aataatcaaa actcaactat ttcactaact caagatgaag
      841 tttttgtttt acgagctatc ttgaatgaga tatatgcggg cgtatgtgta gattcaagag
      901 aatttgaaaa tgtatctggt gttagaaaac atgaagtaga taatttacaa caacagtttg
      961 ctggaattta taaaaaaatg acaacttaac aacccaaatt tttgtcagag cctagtgcaa
     1021 attacaacta tgattctatt gtagccgaaa tgaaagaaaa aaatcatggg ttggcgacag
     1081 ggttgatgtt gttaatatgc ctgatggagc acctactagt atggataaca cgcgtattat
     1141 ggcagcacgt gaagcagg
//
LOCUS       DAXHRI010000262         1114 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXHRI010000262 DAXHRI010000000
VERSION     DAXHRI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.262"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            57..782
                     /locus_tag="ACFM75_002119"
     CDS             57..782
                     /locus_tag="ACFM75_002119"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC6256574.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLADIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          307 a          369 c          247 g          191 t
ORIGIN      
        1 gtccgtttcc atcacgccgc tgcgacagat tataaccgtc cgaaccgcca aaaactatgc
       61 cccgattcta cctgcccgaa aacctttccg tcggacaaac cgtcgccctg cccgacaaca
      121 tcgtccgcca cctcaacgtc ctacgcgtcc gccccaacga aaacatcacc ctcttcgacg
      181 gcaaaggcaa ggcacacgcc gcacagctga ccgttttgga aaaacgccgc gccgaagccg
      241 aaatcctgca cgaagacaca accgacaacg agtccccgct caacatcaca ctgatacaat
      301 ccatctcctc cggcgatcgc atggatttca ccctgcaaaa aagcgtcgaa ctcggcgtaa
      361 ccgccataca gcccgtcatc agcgaacgct gcatcgtccg cctcgacgga gaacgcgccg
      421 ccaaacgcct cgcacgctgg caggaaatcg tcatctccgc gtgcgaacaa agcggcagga
      481 acaccgttcc ccccgtactg cccatcatcg gctaccgtga agcactcgac aaaatgccgt
      541 ctgaaagcac caagctgatt atgagcatca accgcgcccg caaacttgcc gacatccgcc
      601 acccgtccgg cgcaatcgtc tttatggtcg ggcccgaagg cggctggaca gaacaggaag
      661 aacaacaggc atttgaagcc ggctttcagg cggttacact cggcaaacgg attttacgca
      721 cagaaaccgc cccactcgcc gccctcgccg ccatgcagac gctttggggc gatttcgcat
      781 aaacagaaat gccgtctgaa acccgttcag acggcatttt gcagccgatt aagatagtag
      841 gttcaaataa gatttcccgt gtcgtcattc ccgcgaaagc gggaatctag aaacgaaaaa
      901 ctacagagat ttatccgaaa caacaaccct ctccgccgtc attcccgcaa aagcgggaat
      961 ctagaaacga aaaactacag ggatttatcc gaaacaacaa accctctccg ccgtcattcc
     1021 cacgaaagcg ggaatctaga aatttaacgt tgcggtgatt tatcggaaat gactgaaact
     1081 caacggactg gattcccgcc tgcgcgggaa tgac
//
LOCUS       DAXHRI010000263         1088 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXHRI010000263 DAXHRI010000000
VERSION     DAXHRI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.263"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>1088
                     /gene="pilC"
                     /locus_tag="ACFM75_002120"
     CDS             <1..>1088
                     /gene="pilC"
                     /locus_tag="ACFM75_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6256575.1"
                     /translation="AQTYSYVIVMNGQKLPEVKNRNQSSNQDKRRQATFTARFIGAKR
                     NTTFSFDNTDELVAEKKDVVVFGAATYLPPYGKVSGFDDKRLAERKNALDWIGTTAPG
                     LVGYSYQGSTCNSGDCPDVSYKTQFTFGNQGLKRKTNGRLDIDEDKSRNNSLIYKLSD
                     YSWLGVSFNLSSESIVESKKLKKVESSFNEEVTQNNGTFSQYKDKNLIYTTGDVRNRD
                     NQAHRDKPHAVAFYLNAKLHLLDKKQIKDIMRGSELNLGDLKTRIEPTDAWKNKRHRT
                     LTVGQWEFKDTGKISVKLKLPEVKAGRCINKPNPNPKAQALSPALTAPALWFGPVQNG
                     KAEMYSASVSTYPDSSSSRIYLQNLKRK"
BASE COUNT          341 a          255 c          256 g          236 t
ORIGIN      
        1 ggcgcagacg tattcatacg ttattgtaat gaacggacaa aagctacctg aagtaaagaa
       61 taggaaccaa tcgtcgaacc aagacaagcg ccgccaagct acctttacgg caaggtttat
      121 tggagcaaaa agaaacacta ctttctcatt cgataatact gatgagcttg ttgctgaaaa
      181 aaaagatgtt gtcgttttcg gcgcagccac ctacctgccg ccctacggca aggtttctgg
      241 ttttgatgac aaaaggctgg ccgagcgcaa aaatgccctt gattggattg gtacgaccgc
      301 cccggggctg gtaggctaca gctaccaagg tagcacatgc aacagcggag actgtcctga
      361 tgttagctat aaaacccaat ttacctttgg taatcaaggg ttgaaaagaa agacaaacgg
      421 caggctggat atagacgaag acaaaagccg caacaattcg ctcatttaca aattgtcgga
      481 ttattcttgg ttgggcgtgt ctttcaattt gagcagcgag agcatcgtcg aatcaaagaa
      541 actcaagaaa gtggaatctt cttttaacga ggaggtgacg cagaataatg gtacgttcag
      601 ccaatacaaa gacaaaaacc tcatttatac gacaggcgat gtccgtaata gggataatca
      661 agcccatcgg gacaaacccc acgccgtcgc cttttatctg aacgccaaac tgcacctgct
      721 ggataaaaaa caaatcaaag atatcatgcg aggcagtgaa cttaatttgg gtgatttgaa
      781 aacgcgtatc gagccgacgg acgcgtggaa gaataagagg catcgcactt taactgttgg
      841 tcagtgggag ttcaaagata ccggaaaaat cagcgtcaaa ctcaaattgc cggaagtcaa
      901 agcaggccgc tgcatcaata aaccgaaccc caatcccaaa gcccaagccc tttcgccggc
      961 actgactgcc cccgcgttgt ggttcggacc tgtgcaaaat ggtaaggcgg agatgtattc
     1021 cgcttcggtt tccacctacc ccgacagttc gagcagccgc atctaccttc aaaatctgaa
     1081 aagaaaaa
//
LOCUS       DAXHRI010000264         1082 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXHRI010000264 DAXHRI010000000
VERSION     DAXHRI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.264"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            284..721
                     /locus_tag="ACFM75_002121"
     CDS             284..721
                     /locus_tag="ACFM75_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HFC6256576.1"
                     /translation="MSRYLITFDMDTNCLKDNYHGNNYTNAYSDIKTILARHGFENIQ
                     GSVYLGREGISEAHGTIAIQELTARFDWFYSCISNIKFYRLESDLNAQFIADGVYQAK
                     QAFLQRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
BASE COUNT          338 a          248 c          219 g          277 t
ORIGIN      
        1 gccaccaaaa cccttgctag ctaagggtta aacagctcac ttgaaatcta cttaagtcta
       61 atctaaacta tccaatatgg atagattttt aaacataggg caagcagcaa aattattgta
      121 gctgaaagca caatcactcg ctggtggtct caaacacgtg ccgactacct cgccgaaaac
      181 actatcagcc gcgataaacc gtgggaaaag ctcgttatca gccgccgcac ttggtactat
      241 cgcgggaaac cgatgctgtc tgaaacgcaa caggagaaaa ataatgagcc gttacctgat
      301 tacctttgat atggatacca actgcctgaa agacaattac cacggaaata actataccaa
      361 tgcctactcc gatattaaaa ccatcttggc tagacatgga tttgagaaca ttcagggcag
      421 tgtttatcta ggccgtgaag gcatcagtga agcacacgga acaatagcca ttcaggaact
      481 gaccgctcgg tttgattggt tttactcctg tatttcaaac attaagtttt accgccttga
      541 aagtgatttg aacgcacaat ttatcgctga tggtgtgtat caagccaaac aggctttcct
      601 tcaacgtgtt gaacaacttc gtatatccct aacagaagct ggattgtctg atgagcaaat
      661 caatcaggtt ctggaaaaac agaaatttga attggaaagt cctaacctga aattaaatta
      721 acctccttta ctcaccaaca tccgccgcag ctctgtcagt ttttggcgcg ctgcggcgat
      781 ttctgtgcgt tttagagctt cgggtagggt gtgaaacaac tcactcgaaa tttacttaag
      841 tctaatctaa actatccaag cagtaattag tacaaaaaag gcaaacttat tttaggagct
      901 taaaattgca gccgcgataa accgtgggag agtctcggca tttcccgcgc cacttggtac
      961 aaacgtggca aaccgatgcc gtctgaaacc gtacaacagg agacaaaata atgcttgctc
     1021 aaatcgaact cacaccctgc cagctaatgg tttacgtaac ttaaggttac tggatttacg
     1081 ca
//
LOCUS       DAXHRI010000265         1074 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXHRI010000265 DAXHRI010000000
VERSION     DAXHRI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.265"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            7..>1074
                     /locus_tag="ACFM75_002122"
     CDS             7..>1074
                     /locus_tag="ACFM75_002122"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6256577.1"
                     /translation="MILFELRRGNAVLVFVNPLYQVCVLEKQHFPRFVIGESLLPHCM
                     EMLEEAGFADAVRAEPCFQLKNGAAFSWGSRYTEFDFTDKFSDGPGTVYQVRRAVFDK
                     ILIGEAAKQGVEVRFGHGVTAFDNSGDFARLNIETDTGKRYELTAKFVLDASGYGRVL
                     PRLLNLETPSHLPPRQAHFTHIDDNITNPKFDRNKILITTHPQHRDVWIWLIPFGGNR
                     CSIGVVGTPDKLVGESETVLKKFVYECPMLNEILDKAVWENDFPFRSIQGYSANVKSL
                     HGRHFALLGNAAEFLDPVFSSGVTIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMI
                     GVDAFRTYSGLNLNQDKATKPQ"
BASE COUNT          255 a          313 c          276 g          230 t
ORIGIN      
        1 tagagaatca ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
       61 ctatatcaag tctgcgtgtt ggaaaaacag cactttccgc gcttcgtcat cggcgaaagc
      121 ctgctgccgc actgtatgga aatgctggaa gaagccggat ttgccgatgc cgtgcgcgcc
      181 gagccctgct ttcagttgaa aaacggcgcg gcgttttcgt ggggcagccg ttataccgaa
      241 ttcgatttta ccgataaatt ttcagacggc cccggcacgg tttaccaagt ccgccgcgcc
      301 gtgttcgaca aaatcctgat tggcgaagcc gccaaacaag gcgttgaagt acgtttcggg
      361 cacggcgtaa ccgcgttcga caacagcggc gattttgccc gcttgaacat cgaaaccgac
      421 accggcaagc gctatgaact gaccgcgaaa ttcgtcttgg acgcaagcgg ttacggacgc
      481 gtgctgccgc gcctgctaaa cttggaaacg ccctcgcacc tgccgccgcg ccaagcgcat
      541 ttcacgcaca tcgacgacaa cattaccaac ccgaaattcg accgcaacaa aatcctgatt
      601 accacccatc cgcaacaccg cgacgtgtgg atttggctga ttcccttcgg cggcaaccgc
      661 tgttccatcg gcgtggtcgg cacgcccgac aaacttgtcg gcgaatcgga aacggtgttg
      721 aaaaaatttg tttacgaatg cccgatgctg aacgaaattc tggacaaagc cgtttgggaa
      781 aacgattttc cgttccgctc catccaaggc tattccgcca atgtgaaatc gctgcacggc
      841 aggcatttcg cgctattggg caatgccgcc gagttcctcg atcccgtgtt ctcgtcgggc
      901 gtaaccatcg cgctgcactc cgccaaactt gccgccgatc tgttggcaaa acaactcaaa
      961 ggcgaagccg ccgattggca aaccgaattt gccgaaccct tgatgatcgg cgtggacgcg
     1021 ttccgcacct atagtggatt aaatttaaat caggacaagg caacgaagcc gcag
//
LOCUS       DAXHRI010000266         1068 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXHRI010000266 DAXHRI010000000
VERSION     DAXHRI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.266"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            248..418
                     /locus_tag="ACFM75_002123"
     CDS             248..418
                     /locus_tag="ACFM75_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC6256578.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT          331 a          290 c          237 g          210 t
ORIGIN      
        1 ctaacgcaac aggaattcat cgaaaaaacc gaaacctcca aaccgtcatt cccacggaag
       61 tgggaatcca gtaatcgaaa aaccacagga atctatcgaa aaaaacccca acccccaaaa
      121 aaaccgggcg gataccgcac catcctccca aaccctgatt tatagtggat gaacaaaaat
      181 caggacaagg cgaggcaacg ccgtaccggt ttttgttcat ccgctataca aaaacaagga
      241 aacacaaatg gcgcaatata tgtgcggccc ctgcggctgg atttacgatg aagaacacgg
      301 cgaccccgaa cacggcatcc cccctgggac aaagtttgaa gacatccccg acgactggaa
      361 atgccccgaa tgcggcgtag gtaaagaaga tttctacctg ttggatttcg tgatatagcg
      421 gcagccgcac ccttagggaa aaccccgtca tccggcgttc cgcctgcggc ggcagggaac
      481 gccgttttat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
      541 atagtacgga accgattcac ttgttaaaga atcgttctct ttgagctaag gcgaggcaac
      601 gccgtactgg tttttgttaa tccactatat ccgtttgcgc ccgccaagcc gtgttgcaca
      661 gatgcgacac ggcttttttc tttctgttgc cagaatgtaa attttcgtca aaaaacatca
      721 aaaatcaccc cccccccgaa tttgcgtaaa ataaagtgta atccctttga tcataaggga
      781 atgttgacaa ttagatcgat tctcctaggc tgactttcgc aataaatggg gttgtcaata
      841 attgtgtatg gtcgtttgcc gtccggcggc cgtcattccc gcgaacgcag gaatccaaaa
      901 acgcaacgca acaggaattt atcagaaaaa acagaaaccc caccgaccgt cattcccgca
      961 aaagcgggaa tccagaaaca caacgcggca ggactttatc ggaaaaaaca gaaaccccca
     1021 ccaccgtcat tcccgcgcag gcgggaatcc agacccgtcg gcacggaa
//
LOCUS       DAXHRI010000267         1038 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXHRI010000267 DAXHRI010000000
VERSION     DAXHRI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.267"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            11..1012
                     /locus_tag="ACFM75_002124"
     CDS             11..1012
                     /locus_tag="ACFM75_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC6256579.1"
                     /translation="MIDGQTNKPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAENALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRNILAEFEREMNT"
BASE COUNT          294 a          277 c          269 g          198 t
ORIGIN      
        1 tacccaaatc atgattgacg gacaaacaaa caaacccaaa caaaaaaccc gaatcatcct
       61 tgcccccatg caagggctgg tagacgatgt gatgcgcgac ctgctgacgc ggataggcgg
      121 ctacgacgaa tgcgtcagcg aatttgtacg cattacccat accgtgcatt cccgatctat
      181 atggttaaaa tatgtccccg aaatcgccaa cggaaacaaa acgttttccg gcacgccctg
      241 caccgtccaa cttctgggca gcgatgcgga caatatggcg gagaatgcgc tggaagccgt
      301 ccgcttcggt gcgaacaaaa tcgatttgaa cttcggctgc cccgcgccca ccgtcaacaa
      361 acacaaaggc ggcgcaatcc ttttaaaaga gccggaactg atattccaca tcgtcaaaac
      421 gctgcgcgga cgtttgcccg cacatattcc gctcaccgca aaaatgcggc tcggttacga
      481 agacaaaagc cgggctttgg aatgcgcctg tgcgattgcc gaagggggcg catgcggact
      541 gaccgtacac gcgcgtacca aagccgaggg ttacgaaccg ccggcgcatt gggaatggat
      601 aaggaaaatc cgagacagcg tcaatattcc cgttaccgcc aacggcgacg ttttcagcct
      661 gcaagactat atcggcatca aaacaatcag cggctgcaac agcgtgatgc tcggtcgcgg
      721 cgcggtcatc cgccccgatt tggcgcggca aatcaagcaa tacgagaacg gcgggccggt
      781 caaagacacg gattttgccg aagtttctaa atggatacgg cagtttttcg agctgtgcct
      841 gacaaaagag gcaaacaata aatacccgat cgcccgtctt aagcaatggt tgggcatgat
      901 gaaaaaaacg ttcgacccgg cgcaaacgct gtttgaccgc gtccgaacgg ttaaggatgc
      961 ggatgaagtt cggaacatct tggctgaatt tgagcgagaa atgaatactt gaatatgtat
     1021 agtggattaa caaaaacc
//
LOCUS       DAXHRI010000268         1037 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXHRI010000268 DAXHRI010000000
VERSION     DAXHRI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.268"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(301..687)
                     /locus_tag="ACFM75_002125"
     CDS             complement(301..687)
                     /locus_tag="ACFM75_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC6256580.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(684..932)
                     /locus_tag="ACFM75_002126"
     CDS             complement(684..932)
                     /locus_tag="ACFM75_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFC6256581.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
BASE COUNT          285 a          245 c          181 g          326 t
ORIGIN      
        1 cacagccata tatattaagt acctgctctt tatgcgcttt tttaggaaag gaagatcaaa
       61 cggttcaata gcgaagtctt gcccaaattt gatggacagg atatagaata catgcgtgca
      121 taccacaccc tgccaaagtt gggagatgca cttaaggttg cacttgtggt cgccaactat
      181 gcctatacac gcggatttcc cgcgtttata ggccgcgacg gaagtctggc ttttaagcga
      241 agacaataac cgaaaagacc gcctcggcgg tctttcttgt gtacacagtt tttatatttg
      301 tcagcttgat gcattgacaa ctctaattcc atattgcgga atatattcat cgacagtcat
      361 cagttcaaag ccttccgctt gggtttgtgc aatcaacatc ctatcgaaag ggtctttgtg
      421 tatttccgga aggcttccag cctgttttgc atgaaacaga cctataggca gcatttcaaa
      481 atcctcttct tgaagcacat caaaaaactc ttccggtaat ttcaacaacc ccttgttctg
      541 cttgatggaa atttcccaaa tacttgctgc actgacaaag atcgcatttc tcggattttc
      601 tatcagtttg cgtgcagata tccccagttt cttgtcatcc aacaaccacc acagcaacgc
      661 atgggtatca agcagaatct ttctcacaga gccgactcct caaaaaataa agctgccgtt
      721 tcattgtcat cctcaagaat acgtgaaata tccgtatttt ccatatgact gaattttttc
      781 aaccttcctg catttcgtgc cggtttttca ataccgatta gttggacgca aggcttacct
      841 gccttcgcaa taataacaat ttcccctgct tctgctcttt gaatcaattg actcaaattg
      901 gtttttgcct gatgaatatt tgcttgaaac ataacacttc tcatgattag ctaacttaac
      961 taatatacat cattaccaag attttgggaa tctcattaca tatatttgat tatatccgcc
     1021 gttttattca caccttg
//
LOCUS       DAXHRI010000269         1012 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXHRI010000269 DAXHRI010000000
VERSION     DAXHRI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.269"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            111..866
                     /locus_tag="ACFM75_002127"
     CDS             111..866
                     /locus_tag="ACFM75_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6256582.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQSLRLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSQRLSERWRLTLNVGNMWKRYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASVRKGLRIGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTIVYRFPRKDHEYQANLSFWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDETFAKFPKIP"
     gene            897..>1012
                     /locus_tag="ACFM75_002128"
     CDS             897..>1012
                     /locus_tag="ACFM75_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6256583.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYL"
BASE COUNT          254 a          280 c          272 g          206 t
ORIGIN      
        1 ctataaacaa cgcttcgtcc gaaaaaacga ttgaatttgc gggcagaagc tggacgaaaa
       61 ccgccgacag cctgccgcaa aaggcacacg gtttgcgcta gggcttaggc gtgtcgcgcg
      121 aaatcaatgc gggcgggcat catttcctct acggcggcat cagcggcggc ggcgtgcatt
      181 attgggataa caaagatttc agcgaacaga gcctgcgcct gtcgctcggc tataaaaacc
      241 gttcggtaac gcgctcgttc ggcatcgtgc cgtttgtcgg gcaaaacctc ttaggcggca
      301 gccgatacaa tttcgtcggc ggcttcaatg ccgatttctc ccaacgcttg agcgaacgct
      361 ggcggctgac actaaacgtg ggcaatatgt ggaagcgtta tcaggaagac cgcaccgccg
      421 cccgatacga cagccatatg ccgctggcgg gcgcgacgct gatgtattcc gcgccgaaag
      481 actggctgct ttacggcggg gcggactggt cgcacgactt gacgaaggag gcggaacagg
      541 cttccgtccg caagggtttg cgtatcggcg cggtcaaaac gttcgacggc ggcttgggtc
      601 tgcgggcaaa cctgcgctat acacgcagga cgtttgacgc gcccgggacc attgtgtacc
      661 gcttcccgcg caaagaccac gaatatcagg caaacctgtc gttttggcac gacaaaatct
      721 cttggaaggg ctttacgccg caactcaatt tccgctatct gaaaatcgac agcaatatga
      781 aaagttttta cacacgcaaa aacacgcaga ttttcatgag cgtggaaaag gatgagacct
      841 ttgcaaaatt ccccaaaatc ccctaaattc ccaccaagcc atttagggga ttttccatga
      901 gcaccttctt ccggcaaacc gcacaagcca tgattgccaa acacatcgac cgtttcccgc
      961 tattgaagtt ggaccaggta attgattggc agccgatcga acaatacctg aa
//
LOCUS       DAXHRI010000270          983 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXHRI010000270 DAXHRI010000000
VERSION     DAXHRI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.270"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>983
                     /locus_tag="ACFM75_002129"
     CDS             <1..>983
                     /locus_tag="ACFM75_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6256584.1"
                     /translation="TPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTA
                     AGTGVAAGTAATTGVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKEL
                     GKSSTVRQAATAAVTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASV
                     HTAINGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAAN
                     KGKCQDGAIGAAVGEIVGEALVKNTDFSRMSATEIEKAKAKITAYSKLVAGTASAVVG
                     GDVNTAANAAQIAVENNTLYPRCVGAKCDEFQKEQQKWIRENPEEYREVLL"
BASE COUNT          273 a          259 c          284 g          167 t
ORIGIN      
        1 tgacacccgc agcagcagct gtcgtcgtta tcgtcgtaac cgtattgacc tacggtgcac
       61 tgtccgcccc ggcagccgcc ggaacggcgg gcgcggcagg cgcaggagcg ggaggagccg
      121 cagcaggaac ggcagccgga actggagtag cagcaggaac ggcagccaca accggagtag
      181 cagcaggcac atcagctgca gctatcacca cagccgcagg caaagccgca ctggccagtc
      241 tcgccagcca agccgcagtt tccctcatca acaacaaagg agacataaac cataccctga
      301 aagaactggg caaaagcagc accgtcagac aggccgccac cgccgccgta accgcaggcg
      361 tactgcaggg cataagcggg ctgaacaccc aagcagccga agccgtcagc aaacattttc
      421 acagtcccgc agcaggcaaa ctgaccgcta acctgatcaa cagcaccgct gccgcaagtg
      481 tccataccgc catcaacggc ggcagcctga aagacaatct ggaagcgaat atccttgcgg
      541 ctttggtgaa tactgcgcat ggagaggcag ccagtaaaat caaacagttg gatcagcact
      601 acattgccca taagattgcc catgccatag cgggctgtgc ggcagcggcg gcgaataagg
      661 gcaagtgtca ggatggtgcg ataggcgctg cagtcggtga gattgttggt gaggctttgg
      721 ttaagaatac tgatttcagt cgtatgagtg cgaccgaaat cgaaaaagct aaagcgaaga
      781 ttactgccta ttcaaaactg gttgccggca ctgcgtctgc cgttgtaggc ggggatgtga
      841 atacagcggc gaatgcggca cagatagcgg tggagaataa tactttgtat cctagatgcg
      901 ttggtgcaaa gtgtgatgaa tttcaaaagg aacaacaaaa atggatacgt gaaaatcctg
      961 aagaatatcg agaagttttg ctt
//
LOCUS       DAXHRI010000271          982 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXHRI010000271 DAXHRI010000000
VERSION     DAXHRI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.271"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..751
                     /locus_tag="ACFM75_002130"
     rRNA            <1..751
                     /locus_tag="ACFM75_002130"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            848..961
                     /gene="rrf"
                     /locus_tag="ACFM75_002131"
     rRNA            848..961
                     /gene="rrf"
                     /locus_tag="ACFM75_002131"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          240 a          228 c          288 g          226 t
ORIGIN      
        1 gtggagccgt ccttgaaata ccaccctggt gtctttgagg ttctaaccca gacccgtcat
       61 ccgggtcggg gaccgtgcat ggtaggcagt ttgactgggg cggtctcctc ccaaagcgta
      121 acggaggagt tcgaaggtta cctaggtccg gtcggaaatc ggactgatag tgcaatggca
      181 aaaggtagct taactgcgag accgacaagt cgagcaggtg cgaaagcagg acatagtgat
      241 ccggtggttc tgtatggaag ggccatcgct caacggataa aaggtactcc ggggataaca
      301 ggctgattcc gcccaagagt tcatatcgac ggcggagttt ggcacctcga tgtcggctca
      361 tcacatcctg gggctgtagt cggtcccaag ggtatggctg ttcgccattt aaagtggtac
      421 gtgagctggg tttaaaacgt cgtgagacag tttggtccct atctgcagtg ggcgttggaa
      481 gtttgacggg ggctgctcct agtacgagag gaccggagtg gacgaacctc tggtgtaccg
      541 gttgtcacgc cagtggcata gccgggtagc taagttcgga agagataagc gctgaaagca
      601 tctaagcgcg aaactcgcct gaagatgaga cttcccttgc ggtttaaccg cactaaagag
      661 tcgttcgaga ccaggacgtt gataggtggg gtgtggaagc gcggtaacgc gtgaagctaa
      721 cccatactaa ttgctcgtga ggcttgactc tatcatttga agaacttcaa gagataaaag
      781 cttactgact gattcagtca ttaccgaata tattgattaa ggctttaccg atttgtaaca
      841 gtttaagttt ggcggccata gcgagttggt cccacgcctt cccatcccga acaggaccgt
      901 gaaacgactc agcgccgatg atagtgtggt tcttccatgc gaaagtaggt cactgccaaa
      961 caccctttca gaaaaccccc ga
//
LOCUS       DAXHRI010000272          960 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXHRI010000272 DAXHRI010000000
VERSION     DAXHRI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.272"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..242
                     /locus_tag="ACFM75_002132"
     CDS             <1..242
                     /locus_tag="ACFM75_002132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256585.1"
                     /translation="GETTTYRFLLIRYTTMNPSQYLFIDLNFDKIPDTRLCCFSIFQP
                     TVNLKQPVHAMLQFLFLSADCFDKELKIHFMHLKI"
     gene            350..910
                     /locus_tag="ACFM75_002133"
     CDS             350..910
                     /locus_tag="ACFM75_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HFC6256586.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINFPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          271 a          266 c          193 g          230 t
ORIGIN      
        1 aaggcgagac aacgacgtac cggtttttgt taatccgcta taccacgatg aatccttcgc
       61 aatatctgtt tatcgacctc aattttgaca aaataccgga tacgcgcctt tgttgctttt
      121 ccatcttcca accaactgta aatctcaaac agccggtaca cgccatgctt cagtttcttt
      181 tcctgtcggc ggattgtttc gacaaagaat tgaaaatcca tttcatgcac cttaaaattt
      241 aatctgcatt caaacctttt cactttggaa gcaccattta tcggatgtcc cttcgcaata
      301 aacaaatttt cccgataccg ccgcccattt caacccaaac ccaaaagcta tgaaaaacct
      361 catcgccttc aacaaaccct atggcgttat ctgccaattt tcaccgcacg aaaaacacaa
      421 aagcctcaaa gactttatca attttcccgg cttctacccc gccggacggc tcgacaccga
      481 cagcgagggg ctgctgctgc tgaccgacga cggcaggctt caggcacaaa ttaccgaccc
      541 caaattcaaa caccctaaaa cctactgggc gcaactggag ggcgtacccg acgaaagccg
      601 attggaaagc ctaagaaaag ggatagactt aggcggtttc gttacccgtc cggcaagcat
      661 ccgcatcttg aaacacggag aagcagattc gttatgggag cgcatcccgc cgatacgcgt
      721 ccgcaaaacc gttcccgatt tttggattga aattaccatt tctgagggca aaaaccgcca
      781 agtcaggcga atgaccgcca aggcgggcta tccctgcctg cgtctgatca gagtggcaag
      841 cggcagactg aaactgtttg atttggattt aaaacccggg gaatgggcat acgccccgtt
      901 taaaccataa tcacgtttat ctcatcattt ccacaaaagt gggaatccgg aattttatag
//
LOCUS       DAXHRI010000273          954 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXHRI010000273 DAXHRI010000000
VERSION     DAXHRI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.273"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..186)
                     /locus_tag="ACFM75_002134"
     CDS             complement(<1..186)
                     /locus_tag="ACFM75_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256587.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSV"
     gene            complement(183..>954)
                     /locus_tag="ACFM75_002135"
     CDS             complement(183..>954)
                     /locus_tag="ACFM75_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6256588.1"
                     /translation="SPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVENNSLL
                     ARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAIMRNPREPWTRHDVQT
                     YRQYYNQLRESRGIPVLPIYRMRINNGNEFNRIMSSKYPYNELYVANPKSATGYFRVD
                     SYDPATREIISRKFTQFSQIQESTGIGYIKEAVRKYSPGTVISNVPSTPTTIRGRKLE
                     GKLILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
BASE COUNT          252 a          215 c          167 g          320 t
ORIGIN      
        1 taccgaaaaa aaacctactt cctctcgcaa ggtaattaca tattcaggtt gtgtcgccga
       61 acctattacg gcagtatata actccccttt aagatgtttt tcctcagctt gtttctcaga
      121 aaaaggctta tgaagtctct tataaccata agaccactgc tcacaataaa aaatatcttt
      181 cttcatttgt aaatccttcc tgttgtatct ctaatgataa cattttcttc ttgtgccgcc
      241 cttaatacag attgtggaat tggattgacc tgagcaggaa cttctaaaat aagttttcct
      301 tcaagctttc ttcctcttat cgtagtaggt gtacttggaa cattggaaat gacagtacca
      361 gggctatatt ttctaacagc ctccttgata taaccaatcc ccgtactttc ttggatttga
      421 gaaaattggg taaattttct tgaaataatt tcccttgtcg caggatcata cgaatctacc
      481 ctaaaatacc ccgtcgccga tttaggattg gctacataaa gctcattata agggtatttt
      541 gatgacatga tacggttaaa ttcattgccg ttgtttatcc tcattctata aattgggaga
      601 acaggaatgc ctctagattc ccttaattga ttataatatt gcctgtatgt ttgtacatca
      661 tgtcttgtcc acggctctct aggattcctc ataatagcaa tcccgttaaa tttcggatcc
      721 agccttcgga tttgattggt aatggcctgg atttcaagaa tggcatattc atgttccaat
      781 tcttgtcgcg gagtccaacg tatatttacc ctcctgcgag ctaaaagact attattctcc
      841 actgccacag tagccgcatt cgccgccgta ttcacatccc ctttaaccaa tgccactgcg
      901 ctgcctgcga taatctgcga gtaggctatg actttttggc gttcttgggg tgac
//
LOCUS       DAXHRI010000274          941 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXHRI010000274 DAXHRI010000000
VERSION     DAXHRI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.274"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(410..694)
                     /locus_tag="ACFM75_002136"
     CDS             complement(410..694)
                     /locus_tag="ACFM75_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6256589.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          268 a          252 c          170 g          251 t
ORIGIN      
        1 agaatctcta aagcttcagc taacctttga atattactgt tgttctaagg cctagattcc
       61 cgcctacgcg ggaatgacga acccatccat acggaaacct gcaccgcgtc attcccgcga
      121 acctacattc cgtcattccc acgaaagtgg gaatccagga cgcggaatct caagaaaccg
      181 tttacccgat aagtttccgt gccgacagac ctagattccc gcctgcgcgg gaatgacggt
      241 ttagaagttg cccgaaatct caaaaaaaac cgaaaccgaa caaaccggat tcccgcaacc
      301 tttcgtcatt cccgtgaaaa cgggaatcta gaacctcaaa ctttcagata atctttgaat
      361 attgctgttg cccaatggtt tggattcccg catgcgcgag aatgacgggc taaataatat
      421 caaaccataa atcctgccaa gaaacattat tttcttcaat cagttgcaat ttccaagccc
      481 tgttccattt cttcaactgt ttttcccgag taattgcgct ctccatcgta ggatgcagtt
      541 cataccaaac cagcatagta acgttgtacc gtgatgtaaa tccctcaatc aaatgttccc
      601 tatgttggta aatacgttgc accaaatcag atgtaacgcc aatgtataac gtgccattac
      661 gttggcttgc taaaatataa accgcaggct gcatataaca cccttttgaa ttatttaaat
      721 ttatattccc gcgaacacca tcccgtgatt actttaaccc ttcgttattc ccatagcttt
      781 ccatcattcc cgcaactctt cgtcattccc gcgaaagtgg gaatctagaa cgcaaaatct
      841 aaagaaaccg ttttacccga taagtttccg caccgacaaa cctagattcc cgcctgcgcg
      901 ggaatgacgg cggagcggtt tctgtttttt ccgataaatg c
//
LOCUS       DAXHRI010000275          926 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXHRI010000275 DAXHRI010000000
VERSION     DAXHRI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.275"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..807
                     /locus_tag="ACFM75_002137"
     CDS             <1..807
                     /locus_tag="ACFM75_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HFC6256590.1"
                     /translation="PLAAMRHILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILRS
                     VNSTDPEPFFFPCDMLNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLN
                     RHMIYNTLLMVEGKQLDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMK
                     AALEPLFDKKTARMAAKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIV
                     YDPLPADEYQDIFDFMKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT          256 a          217 c          204 g          249 t
ORIGIN      
        1 ccgcttgcag cgatgagaca tattttaatg tatactgaag ctgcggcttc ccatgcctat
       61 ctatttgaac atgatcttaa gaaattcaag caatatgctt atgttgcagg aaagctgggt
      121 attttgcgga gtgtaaattc tacagaccct gaacccttct tctttccctg tgacatgctc
      181 aacattcaag atccgatgtt tctgatgctg atgagcgaca gcccgcagct gcgtgagttt
      241 ctggtgcgca atatcgacaa catcgccaac gatacagaag ccttcgtaaa ccgctacgac
      301 ctcaaccgtc atatgattta caatactctg ctgatggtgg aaggtaagca gcttgatcgg
      361 ttgaaacaac gtagcgagaa agtcttggcg catcccaccc ctagcaaatg gctgcaaaag
      421 cggttgtacg attaccgctt cttcctcgct ttcgccgaac aggatgccga ggcgatgaaa
      481 gccgccttag agccgctttt cgataaaaaa accgcgcgta tggctgccaa agaaacattg
      541 tcctatttcg atttctacct gcagccgcaa atcgttacct acgccaaaat cgcatccatg
      601 cacggtttcg atttgggcat agatcaagaa atcgcaccga gggatttgat tgtttacgat
      661 ccgctgccgg cagacgaata tcaagacatc ttcgatttta tgaaacagta cgacttgtct
      721 tacccgtatg aatatctgca ggattggata gattactata cgttcaaaac cgataagctg
      781 gtatttggta acgcgaagcg agagtgagcc gtaaaactct gagctcctgt tttatagatt
      841 acaactttag gccgtcttaa agctgaaaga ttttcgaaag ctataaattg aaacccttcc
      901 acagtacata gatctgtgtt gtggcg
//
LOCUS       DAXHRI010000276          900 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXHRI010000276 DAXHRI010000000
VERSION     DAXHRI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.276"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(87..710)
                     /gene="mtrR"
                     /locus_tag="ACFM75_002138"
     CDS             complement(87..710)
                     /gene="mtrR"
                     /locus_tag="ACFM75_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC6256591.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEGGSWAVFRHTLLHFFERLQSNDIY
                     YKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKETAV
                     IFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          202 a          200 c          236 g          262 t
ORIGIN      
        1 tgtattttta gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt
       61 cagacggcat tgctaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag
      121 ttgtccatca ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag
      181 gaagagaacc accgccaaat cagcccgtcc aaggttgatt tgatgaagat aactgccgtt
      241 tccttgtcca aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta
      301 attttctcgc gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc
      361 tccgtgtgtt cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt
      421 tgcagccgct cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg
      481 gcatcttgcg cgatgcagtt ttcgatgtcg tcgcagatac gttggaacag cgcgtcgaac
      541 aagtcttcct tatttttgaa atgccaatag agcgcgccgc gcgttacgcc ggcggcttgg
      601 gcgatttcgt tgagcgaggt gcgcgcaatc cctttgcggt aaaaggtttc caaggcggca
      661 agcatcaggt gttctttggt tttcaaggct tcggttttgg tttttctcat aatggcgttt
      721 tcgtttcggg tcggtttgat gagggcggat tataaaaaag actttgtaac catgcaatcg
      781 tgtatgtata atgaaaccca tgaaattgag actacatctc aactttgaaa acaggcataa
      841 cctgctttgc aacccgttcg aacattcggg tatcaaagca ggcggttttt atatcctgaa
//
LOCUS       DAXHRI010000277          872 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXHRI010000277 DAXHRI010000000
VERSION     DAXHRI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.277"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            4..810
                     /locus_tag="ACFM75_002139"
     CDS             4..810
                     /locus_tag="ACFM75_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6256592.1"
                     /translation="MNPAPKKPSLLFSSLLFSSLLFSSAAQAASENSSRSPYYVQADL
                     AYAAERITHNYPEPTGADKDKISTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAADYAS
                     YRKWKESNSSTKKVTEEINNNYKETQTKHQGNGSFHAASSLGLSAIYDFKLNDKFDKF
                     KPYIGARVAYGHVKHQVHSVESKNTIVTSKPTQGAAQGGPVPTGFVPKSAYHESHSIS
                     SLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT          245 a          255 c          176 g          196 t
ORIGIN      
        1 aaaatgaatc cagcccccaa aaaaccttct cttctcttct cttctcttct cttctcttct
       61 cttctcttct cttccgcagc gcaggcggca agtgaaaaca gcagccgcag cccgtattat
      121 gtgcaggcgg atttagccta cgccgccgaa cgcattaccc acaattatcc ggaaccaacc
      181 ggtgcagaca aagacaaaat aagcacagta agcgattatt tcagaaacat ccgtgcgcat
      241 tccatccacc cccgggtgtc ggtcggctac gacttcggcg gctggagaat agcggcagat
      301 tatgccagtt acagaaaatg gaaagaaagt aattcttcta ctaaaaaagt tactgaagag
      361 ataaacaaca actacaaaga aacccaaaca aaacatcagg gaaacggcag cttccacgcc
      421 gcttcttctc tcggcttatc cgccatttac gatttcaaac tcaacgataa attcgataaa
      481 ttcaaaccct atatcggcgc gcgcgtcgcc tacggacacg ttaaacatca ggttcattca
      541 gtggaaagca aaaacacgat tgttacctct aaaccaacgc aaggcgctgc acagggaggt
      601 cctgtcccaa caggttttgt ccccaaatct gcctatcacg aaagccacag catcagcagc
      661 ttgggtcttg gtgtcatcgc cggtgtcggt ttcgacatca cgcccaagct gaccttggac
      721 accggatacc gttaccacaa ctggggacgc ttggaaaaca cccgcttcaa aacccacgaa
      781 gtctcattgg gcatgcgcta ccgcttctga ttccccgata ccgatgccgt ctgaaccttc
      841 agacggcatt tttgatttac ctgccgttga ca
//
LOCUS       DAXHRI010000278          872 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXHRI010000278 DAXHRI010000000
VERSION     DAXHRI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.278"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(55..834)
                     /locus_tag="ACFM75_002140"
     CDS             complement(55..834)
                     /locus_tag="ACFM75_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC6256593.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          202 a          288 c          173 g          209 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcgaccaaa acattcaatg ccgacctgac cgacgggcgg gacacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttcca cgatgcggcc
      781 ttccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg ccgaaccctt cc
//
LOCUS       DAXHRI010000279          865 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXHRI010000279 DAXHRI010000000
VERSION     DAXHRI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.279"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            144..530
                     /locus_tag="ACFM75_002141"
     CDS             144..530
                     /locus_tag="ACFM75_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6256594.1"
                     /translation="MKGLDKYILFKNCETLGELFRIYQDYLSLNIDDPNLKIKFFDLL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRKIWPDMEEMLLYFPDNPWHYVEWF
                     WWGATCPIELTQLPKIEIYEEQMKQA"
     gene            579..>865
                     /locus_tag="ACFM75_002142"
     CDS             579..>865
                     /locus_tag="ACFM75_002142"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6256595.1"
                     /translation="MHITGSDVAGKEGTGLIADKQIHLQSAEQSNTERSQNKSAGWNA
                     GAAVSFGQGGWSLGVTAGGNIGKGHGNGDSITHRHSHIGDKGSQTLIQSGG"
BASE COUNT          307 a          162 c          186 g          210 t
ORIGIN      
        1 ccttggaaag gaacgaccat cattttaagg aatttttcaa ctagccgaga acaaactgga
       61 gcaaaatgga cgattgagtt tcaaaatcat ccattagtag gtaggaaaaa aatggaatta
      121 aaatttagat aaggaattga ataatgaaag gtttagataa gtatatccta tttaaaaatt
      181 gtgaaacatt aggagaattg tttcgaatat atcaagacta tctatcatta aatattgatg
      241 acccaaattt aaaaattaaa ttttttgacc ttttggaaga acttttatca gatggtgtta
      301 ttgaattatg cgattatcgt gaaaatcctc caaaaatatt aaccggttcg ccaaaaacac
      361 aggtagatga attaagaaaa atttggccgg atatggaaga gatgttgcta tattttccag
      421 ataacccttg gcattatgtt gaatggtttt ggtggggagc aacttgccct attgaactga
      481 cacaattgcc taaaatagaa atttacgaag aacaaatgaa acaagcttaa agccagcgta
      541 accagcctgt atgacaaggt gcaggtgaac aatccaatat ccacattacc ggctcagatg
      601 ttgccggcaa agaaggaacc ggcctgattg cagataagca aatccatctg caatcagccg
      661 agcaaagtaa taccgaacgc agccaaaaca aatcggcagg ctggaacgca ggtgctgccg
      721 tatcattcgg acaaggaggc tggtcattag gcgttaccgc aggcggcaat atcggcaaag
      781 gccacggcaa tggcgacagc atcacccacc gccatagcca tatcggcgac aaaggcagcc
      841 aaacccttat ccaaagtggt ggcga
//
LOCUS       DAXHRI010000280          819 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXHRI010000280 DAXHRI010000000
VERSION     DAXHRI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.280"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>819
                     /locus_tag="ACFM75_002143"
     CDS             <1..>819
                     /locus_tag="ACFM75_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFC6256596.1"
                     /translation="QSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAK
                     PDAVTLSASQGIDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQ
                     KRRRFLGISYSKAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATI
                     RAGVGEQARADAKIILEGIKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFTGPV
                     APVLTAPGGYIADIPKGNLKTEIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDR"
BASE COUNT          264 a          248 c          180 g          127 t
ORIGIN      
        1 caatcttacg gtgaccaata ttactatgcg agacagagcg aactctatac ctttgaacgc
       61 cgcagctaca aaaccggcaa atggtacaac cgcaaacaca ttaccgaagt caaagaacac
      121 aaaaacgcca agcccgacgc agtaaccctc agcgcatccc aaggcatcga catcaaatct
      181 ggtggcagca tcgacgccta cgccaccgca ttcgatgccc ccaaaggcag cattaacatc
      241 gaagccgggc ggaaattgac actctatgcc gtagaagagc tcaactacga caaactagac
      301 agccaaaaaa ggcgcagatt tctcggcatc agctacagca aagcacacga caccaccacc
      361 caagtcatga aaaccgcgct gccctcaagg gtagttgcag aatcagccaa cctccaatcg
      421 ggctgggata ccaaactgca aggcacacag tttgaaacca cactgggtgg cgcaaccata
      481 cgcgcaggcg taggtgagca ggcacgggca gatgccaaga ttatcctcga aggcatcaaa
      541 accacgatcc acaacgaaac cgtgagcagc agcaaatctg ctctatggca aaaacaggca
      601 ggacggggca gtaacatcga aaccttgcaa ttgccgagtt tcaccggtcc cgttgcgccc
      661 gtactgaccg ctcccggcgg ctatatcgcc gacatcccca aaggcaacct caaaaccgaa
      721 atcgaaaccc tcaccaagca gcccgagtat gcttatttga aacaacttca agttgcgaaa
      781 aacatcaact ggaatcaggt gcagcttgct tacgacaga
//
LOCUS       DAXHRI010000281          815 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXHRI010000281 DAXHRI010000000
VERSION     DAXHRI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.281"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..241)
                     /locus_tag="ACFM75_002144"
     CDS             complement(<1..241)
                     /locus_tag="ACFM75_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6256597.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAVLFY
                     RKIRTVINHHLALAADEVFEGPVEQDESDFGGRRKG"
BASE COUNT          259 a          146 c          162 g          248 t
ORIGIN      
        1 tgcctttacg ccgtccgccg aaatcgcttt cgtcctgctc gacagggccc tcaaaaacct
       61 catcggcagc caaggccaaa tgatggttga taaccgtgcg gattttacgg tagaacagta
      121 ctgccgaatt gggatggata cccaaaatat cggcggcaga acgggcggta acttccagca
      181 caaaaaaacg gagcagttct ttctgtactt ttttctttaa tttgcagtgc gttatcttca
      241 tatttcgagg gtaacatatc tgctaatgta gtacagcccc aaaaatatac caaaaacagc
      301 aaaacaaatt gtaaggatac gtataggctt tgtaaaggta aattgtgaaa aaagcagttt
      361 tttaaacgaa tgaaacggct tcgggctgaa atatatgctg atgccctgtt cttcccgtat
      421 ttctcgtgtg ttgtcaaagt gcaggctgct ttgaaatcgg tattgccatc tatgaaccac
      481 cactttgctt tatttcagcg ggcttgagat gtgtataaga atattgtttt gaataaattt
      541 aaagaaaatg ataatcgtta ttgacgattt ttaaaggaaa gcgtagagtg ccaattctat
      601 gaagcaatac ggtaagtaac aatgaaaata tctactgctt gggtatagag catatttcac
      661 aacccgtaac tattcttgcg gaaacagaga aaaaagtttc tcttctatct tggataaata
      721 tatttaccct cagtttagtt aagtattgga atttatacct aagtagtaaa agttagtaaa
      781 ttatttttaa ctaaagagtt agtatctacc ataat
//
LOCUS       DAXHRI010000282          784 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXHRI010000282 DAXHRI010000000
VERSION     DAXHRI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.282"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(225..596)
                     /locus_tag="ACFM75_002145"
     CDS             complement(225..596)
                     /locus_tag="ACFM75_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6256598.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(593..>784)
                     /locus_tag="ACFM75_002146"
     CDS             complement(593..>784)
                     /locus_tag="ACFM75_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256599.1"
                     /translation="HNFNDRLSSKERIRFKHDGIEPQTWGEAIQLRIRKQETQKGVPE
                     GWSKRFPNGSIYDVKVLRK"
BASE COUNT          226 a          151 c          130 g          277 t
ORIGIN      
        1 gaattaatgc cgtcttttac cgcaaaagca ctgtcctgta ataggctcgc ggcagcaagt
       61 tgtgcttccg gacttaaatt tcctaattca ttaatatctt gtaaagtttg ctgcgcagag
      121 atgtaggtcg gatatttgta tccgacaaaa atatttgacg catctatcgt ttccgaaatg
      181 ccgctgttgg aaaatgtcgg attcgagaat ccgacctacg gctgctacca accatcattt
      241 tcagcttctt ttagccaagt atcaaaattt tcaaaagtct cgtaatattt tgtattatct
      301 aaattaacta ctataatttt aggattacct gatttatctg aaatatcaaa acaggcagcc
      361 cactcttggt ttctagcaaa aggtaatagg tttggaatgc cagttatttc aaaataaatg
      421 tcaattactt ctgatatatt gctttctgca ttttcaaacc accaaggaaa agaatattgt
      481 tcatcgtagt taatggcatg agtggattga gctaatttta aataactttg cggatattta
      541 aatcctttag gaagcattgc ttcaggatac ggaacaaaac tattttgttt tatcatttcc
      601 taagtacctt tacatcataa atacttccgt taggaaatct tttgctccac ccttctggaa
      661 ctcctttttg tgtttcttgc tttctaattc gtagctggat agcttctccc caagtttgag
      721 gctcaatacc atcatgctta aacctgattc tctcttttga tgataatcgg tcattaaaat
      781 tatg
//
LOCUS       DAXHRI010000283          779 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXHRI010000283 DAXHRI010000000
VERSION     DAXHRI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.283"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            253..>779
                     /locus_tag="ACFM75_002147"
     CDS             253..>779
                     /locus_tag="ACFM75_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6256600.1"
                     /translation="MPEHIKEEIKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVEN
                     NLYLTSEALKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDS
                     RFKDLKKESLYYLNQHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVL
                     SGVAGGGSCAETFGLG"
BASE COUNT          235 a          178 c          204 g          162 t
ORIGIN      
        1 aatgccgctg cgccttatgc ctccgaagca ataggaagaa catttggaca cggtgaaaac
       61 aaaaacgaaa ccgcccaagc cgtcggacat ttccttttag gagcagctat tgcccgcgtc
      121 aacggtggta attttgctgc cggcggctcg gcagcagttg cagctgaaaa ggcggcggaa
      181 catcttgccc aacagtataa cgacggtaaa accgcaatcg atccgcaaac aggcgagttc
      241 aatgccaacc tgctgccgga acatatcaaa gaggaaatca aatcaaagag cggggtgatt
      301 gcatcgctga cgggcgcggc cgtgggcggc acgccggtag atgcgcaaac cggaggtgcg
      361 gtcggacaga atgcggtgga aaacaacctc tatctgacat cggaagcctt aaagaaggac
      421 aagcagacag ctcgtaaaat ttattccgtc ataaaagagc aagtcaagca tgaatgcagt
      481 tccacaggaa gaattaccga atgtcgtcaa aatataggac gcattatcga atttacccaa
      541 gacaaacgct ttgacagtag gtttaaggac ttaaaaaaag aatccttata ttacctaaat
      601 caacatcctg atttagtagc ctcttatttg aaggctgaat acgaaaagct ggatagggaa
      661 gacaaaagta tcctgcaccg ctacatctca cctggggctg aaatcgtttc gggcagtttg
      721 ggggttgttc tttcaggagt agccggaggc ggatcttgtg ccgagacttt cggcttagg
//
LOCUS       DAXHRI010000284          726 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXHRI010000284 DAXHRI010000000
VERSION     DAXHRI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.284"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(44..610)
                     /locus_tag="ACFM75_002148"
     CDS             complement(44..610)
                     /locus_tag="ACFM75_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC6256601.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGS
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          209 a          246 c          142 g          129 t
ORIGIN      
        1 caacgacgta ctggtttttg ttaatccact atattttccg acatcattga atcaaaccca
       61 aatgcgacaa gagcgtccat gtgccgatgg caatcaacac caaacctccg gcaaattccg
      121 cacgcctgcc gaacaatacg cccaaagccc ttcccgccgt cagcccgacc gccaccatca
      181 ccgtcgtcgc cataccgatg attgcggcgg caaaggcgat gtttacctcc ataaacgcca
      241 agcccacccc gactatcatg gaatcaatac tggttccaaa agcagtcaaa accgtcatcc
      301 ataggctttc ccgtttgctt tcgcgcacat cttccgcctt gccggacagc ccttcgcgca
      361 tcattttcag acccagcccg cccagcagga cgaaagccgc ccaatggtcc cattcgctga
      421 taaacggttt ggcataaaaa ccgcctaccc agcctgccag cggcgtgagt gcttcaaccg
      481 agccgaacac caaggccgtt gctgcaattt tgcgcggagg catcctgacc gccgcaccct
      541 ttgccaatgc gacggcaaac gcatccatcg acatccccag cgcaatcaag agcaaagcgt
      601 aaagactcat actgcacccg tcctcaaaaa gggcagatta tagcaaaaca gcaaagcgcg
      661 aaaaacgccg cacgaaaacc cacatcccgt cattcctgca aaaacaaaaa atcaaaaaca
      721 gaaatc
//
LOCUS       DAXHRI010000285          725 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXHRI010000285 DAXHRI010000000
VERSION     DAXHRI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.285"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(128..>725)
                     /locus_tag="ACFM75_002149"
     CDS             complement(128..>725)
                     /locus_tag="ACFM75_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFC6256602.1"
                     /translation="LSIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          162 a          207 c          198 g          158 t
ORIGIN      
        1 caaacacagt caaacaaatt tatatgcccc atcccttccg aataatttga aaacacagcc
       61 gccaaaaaca aaaatgccgt ctgaaaacct ttcagacggc atttccaatt tgatttcagg
      121 cagaaagtca gaacgcgata tagctgttcg ggttaaccgg tttgccgttt tgccgcacct
      181 cgaaatgaag ctgcgttctg gaagcgtcgg tattgcccat caaagcgacc tgctgcccgc
      241 gtttgacctg ctggccttcg ccgaccagca atttttggtt gtgcccgtat gcggtcagga
      301 aggaagaatt atgctggatg atgaccagat tgccgtatcc cctcaaaccg gaacctgcat
      361 aaaccacttt gccgtcagcc gccgccaaaa cgggctgtcc cgcattaccg gcaatatcga
      421 cacccttgtt gttgccgccg aaatcggcaa ccactttacc ttgcgtcgga cgctgccaaa
      481 caatgccgcc gaccgaacgc gtgccggaag gcgaagcggc aggagattgc ggggcgggcg
      541 cgggaaccgc tttattttcc gcagcgggcg cggcaggttg cggcgcggac tgcacaggcg
      601 gttgcgcggc gggtttcaca ggggtttgca cggcagccgg tacggcgggc ctgctttcta
      661 cggctgaggt tttcggtgcg gcatatcctg ccggtttgac tttaacaatc tgaccgatgc
      721 tcaac
//
LOCUS       DAXHRI010000286          702 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXHRI010000286 DAXHRI010000000
VERSION     DAXHRI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.286"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            544..>702
                     /gene="pilE"
                     /locus_tag="ACFM75_002150"
     CDS             544..>702
                     /gene="pilE"
                     /locus_tag="ACFM75_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HFC6256603.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSA"
BASE COUNT          239 a          197 c          110 g          156 t
ORIGIN      
        1 ccgacgaggc tggattcccg cctgctcggg aatgacgaag ccatccgcac ggaaacctgc
       61 cgcgatgatt caactgccgt ttgcacagga acaccacgcg ccgatttcaa tcactttcca
      121 agaaaacgga aatttttaaa aattaaattt aaaatttccc acccaaccca ccctatgcta
      181 ccgcgcaaat tcaaaaatct caaattccga cccaatcaac acacccgatg ccccatgcca
      241 atagaaatac cccacgccaa taaaaaagtg ccgaaaatcg gcactaaaac tgacaatttt
      301 caacactcct gacaaaaaac ggtaaccccc tgcttcctca aaccacaccc aaacgaaaga
      361 aaatgtaaaa taaaaacaac catataaaga taaacgcata aattttcacc tcaaaacaca
      421 aacttgatac caatcttgct ttataataca caggtatcgc aacaaaatac cgatggtcaa
      481 atacattgca taatgccgat ggcgtaagct tgaggcattt cctttccaat taggagtaat
      541 tttatgaaca cccttcaaaa aggttttacc cttatcgagc tgatgattgt gattgccatc
      601 gtcggcattt tggcggcagt cgcccttcct gcttatcaag actacacagc ccgcgcacaa
      661 gtttccgaag ccatcctttt ggccgaaggt caaaaatcag cc
//
LOCUS       DAXHRI010000287          701 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXHRI010000287 DAXHRI010000000
VERSION     DAXHRI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.287"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..349
                     /locus_tag="ACFM75_002151"
     CDS             <1..349
                     /locus_tag="ACFM75_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256604.1"
                     /translation="TYPQLVNQLTGQNLKNIAAQDPRLSLALHKSEKNFPIGTATYEE
                     ADRLGKIWVGEGARQTSGGGWLSIDGTRQYRPPTEKNSQFATTGIQANFETYTIDSNG
                     KRNKIKNGHLNIR"
     gene            354..>701
                     /locus_tag="ACFM75_002152"
     CDS             354..>701
                     /locus_tag="ACFM75_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6256605.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLN"
BASE COUNT          273 a           85 c          120 g          223 t
ORIGIN      
        1 aacttatcct caattagtta atcaattaac tgggcaaaac ttaaaaaaca ttgcggctca
       61 agatccaaga ttgagtctag ctcttcataa gagtgaaaaa aattttccaa taggaactgc
      121 aacttatgaa gaggcagata ggctaggtaa aatttgggtt ggtgagggtg caagacaaac
      181 tagtggaggc ggatggttaa gtatagatgg cactcgacaa tatcggccac caacagaaaa
      241 aaattcacaa tttgcaacta caggtattca agcaaatttt gaaacttata ctattgattc
      301 aaatggaaaa aggaataaaa ttaaaaatgg acatttaaat attaggtaat tatatgagat
      361 taaaattaaa aaattatgat tgttctgact ttgatttgga agatttccca caagacaagt
      421 tagaaaattt ttgtatactt ttgacattat caataggttt tgatgaaagt aatggtgctg
      481 attattttta tgtttatata tatagcactg aatggttact ttcaaatata catagaccga
      541 tgtcattaaa aaactcaatt gttacaaacc gttttaatat tgaacacata ttaaaattga
      601 taaatgatat attggaaata tgtaattcaa cgtctgaaga taagtctata tcaaatttgg
      661 caaaatattt tgactgggaa tttgacgatt ataatctaaa t
//
LOCUS       DAXHRI010000288          698 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXHRI010000288 DAXHRI010000000
VERSION     DAXHRI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.288"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..464
                     /locus_tag="ACFM75_002153"
     CDS             <1..464
                     /locus_tag="ACFM75_002153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256606.1"
                     /translation="LTGKTAAQTKADIDTNVTTDTAERHSGSLKNVFDKDRVQSELDL
                     QRTVSQDFSKNVQQANTEINQHLDKLKADKEAAETAAAEALANGDMETAKHKAHEAQD
                     AAAKADNWQQGKVILNMLASGLAAPTQSGAGIAAATASPAVSYAIGQHFKD"
BASE COUNT          242 a          168 c          179 g          109 t
ORIGIN      
        1 ggctgacagg aaaaacagcg gcacaaacca aagccgatat tgatacaaac gtaaccacag
       61 acaccgccga acgacattcg ggcagcctga aaaacgtatt tgacaaagat agagtgcaaa
      121 gtgaactgga tttacaaaga accgtcagcc aagattttag taaaaatgtt caacaagcca
      181 ataccgagat taaccaacat ttagacaaac tcaaagcaga caaagaagca gccgaaacag
      241 cagcagccga ggcattagcc aatggcgata tggaaacagc caaacacaaa gcccatgaag
      301 ctcaagatgc ggcagcaaaa gcagataatt ggcaacaagg caaagtcatt ctcaacatgt
      361 tagcctcagg tttagctgcg ccgacccaaa gcggagcggg catcgctgcg gctaccgcat
      421 cgccagccgt atcgtatgcg attggacagc actttaaaga ttgagccggt caaaacgcga
      481 atggcaaact aaccgccagt caagaaaccg ctcacgttct tgcccacgcg gtattaggag
      541 cagcggttgc agcagcagga ggcaacaatg ccccggcggg agcattgagt gcgggcgggt
      601 cggaagcggc tgcgccttac atcagcaaat ggttatatgg caaagaaaaa ggaagtgact
      661 taacggcgga agagaaagag actgagacct ttgcaaaa
//
LOCUS       DAXHRI010000289          693 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXHRI010000289 DAXHRI010000000
VERSION     DAXHRI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.289"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(102..413)
                     /locus_tag="ACFM75_002154"
     CDS             complement(102..413)
                     /locus_tag="ACFM75_002154"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFC6256607.1"
                     /translation="MYEIEQVAQLSSGYAMRLYEFFMQYFDKQTGKGWLEVSLVDLRF
                     RFGLLPNEYARIGNFKTRVIDYSINEINKKTDLTATYEQRKNGRVITGFRFEFTRKQQ
                     Q"
     gene            complement(400..>693)
                     /locus_tag="ACFM75_002155"
     CDS             complement(400..>693)
                     /locus_tag="ACFM75_002155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256608.1"
                     /translation="IDKELTICADDYINTFKVEKHTAYGSLKNAVMGLFEAKWGYQYI
                     NEKVILLFVMKNLLNQHNMLGMKDLYDLLFQLQSFLNHSFFNGDRKTVYRVRN"
BASE COUNT          217 a          138 c          110 g          228 t
ORIGIN      
        1 tcatcaattc gggcttgaat ggctttgcac tcaacatctg aaaaatcggc gaacaaatca
       61 agctcgggcg caacttttgg cagttcagga actttttcag gctactgctg ttgctttctc
      121 gtgaattcaa agcgaaaacc agtaataacg cgcccatttt ttctttgttc gtaagtagcg
      181 gttaaatcag ttttcttatt gatttcattg atagaataat caataaccct agttttaaaa
      241 tttcctattc ttgcatattc attaggaagt agaccaaaac gaaaacgcaa atcaacaagg
      301 gaaacttcaa gccagccttt tcctgtttgt ttatcaaagt actgcataaa aaactcgtac
      361 aagcgcatag cataaccact agacaactgt gcgacttgct caatttcgta cacggtaaac
      421 cgtttttcta tctccattaa aaaaagaatg attaaggaat gattgcagtt gaaaaagtaa
      481 atcgtacaag tccttcattc ctaacatatt atgctgattg agtaaattct tcataacgaa
      541 cagcaatatt actttttcat tgatgtactg ataaccccat tttgcttcaa ataatcccat
      601 gactgcattt tttaggcttc cataggcagt gtgtttttct accttgaaag tgtttatgta
      661 atcatctgca caaatagtta gctctttatc tat
//
LOCUS       DAXHRI010000290          673 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXHRI010000290 DAXHRI010000000
VERSION     DAXHRI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.290"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(89..640)
                     /locus_tag="ACFM75_002156"
     CDS             complement(89..640)
                     /locus_tag="ACFM75_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256609.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          187 a          270 c          125 g           91 t
ORIGIN      
        1 aagaaaccgc cattagcact acaccccccc aagctgcggc tgccaaacac atcggctgct
       61 tctcgacgca cccgctgaaa aatgcgtcct atccgtccgc cttgcccgca ccccctgact
      121 ttgccgccct gcccgatgcc gtctgaagcc tgaaccgccc caccagcccg atgacgcacg
      181 acaccgccgg aaagggcata cacaaagccg tcagcacata acgcgcattc cccgtattga
      241 ccacgtccgc gcccttcagc agcagcggca gcgcgttcgc ccactcccac accgacggcg
      301 cgctgaacgc caataccgcc accagcccca acatccggaa caccccgatt tccaacacct
      361 gccgccgccg caccgaacgg ttgagcaaaa acacaaccgc caacccgaaa cagaccaccg
      421 ccgccatacc gccgttggcg acaatctgca aactgttgaa tgcaatatcg ggcgtaaccg
      481 ccaacacgcc ttccgaatcc ggcacggcac gctgcaaatc caaaccctgc aacacattca
      541 acacaaaacc gtagatcaaa tcaaaaaaca ccacgcctat cggcaaagca ctcaaaaccc
      601 gccacatctt cctatccgac caaccgttca aaactttcat tttacccgac cacgcaagcc
      661 gccgaacaaa aaa
//
LOCUS       DAXHRI010000291          654 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXHRI010000291 DAXHRI010000000
VERSION     DAXHRI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.291"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(53..538)
                     /locus_tag="ACFM75_002157"
     CDS             complement(53..538)
                     /locus_tag="ACFM75_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC6256610.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          191 a          208 c          121 g          134 t
ORIGIN      
        1 tttcaaaccg gaaaaatgcc gtctgaatct tcagacggca ttccattcat atttattcgt
       61 cttttttgtg ttcgatggcg tttcgggaac gattgccgcc gtaaggctct tcaaccgtat
      121 attctccctc gataatatcg tcatcgcggg aaaagccctc ttttctgccc gattggttca
      181 tgttgaaaaa attttccgca cctcctgcct gcaacactgc acctccctta aacggcagca
      241 gcagcaatac cgccaacacc gaggatacga atcccggact catcagacac accgccgcca
      301 ccgtataacg gataggccac aacatctgat aaacggatac cctcccgccg cttctcattg
      361 ccgcgcccgc caataaaaga ccggacagcc ccgtatgcct gagcatcagc acaccggcgg
      421 caaaggttgc cgccatcaga aacagcgtcc aaccgccgcc cagccaatcg gcaacccaca
      481 caatcgacat aatctccaaa aacagcagca ccaaaaaacc gataccgaaa aatctcattg
      541 accgtcatcc ttatatttaa gtaaacagca aaccgcccga acaggactcc aagcgacctg
      601 cctgtaaatg attacaaaac catgtgcttc aagccgaaac aatgtgaaat cacg
//
LOCUS       DAXHRI010000292          644 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXHRI010000292 DAXHRI010000000
VERSION     DAXHRI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.292"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            1..402
                     /locus_tag="ACFM75_002158"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACFM75_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          180 a          194 c          149 g          121 t
ORIGIN      
        1 aatgacgaag ccattctttt ggctgaaggt caaaaatcag ccgtcaccga gtattacctg
       61 aatcacggcg aatggcccgc caacaacagt tctgccggcg tggcatccgt ctccaccgac
      121 atcaaaggca aatatgttca aagcgttaca gtcgcaaacg gcgtcattac cgcccaaatg
      181 gcttcaagcg gcgtaaacaa agaaatccaa ggcaaaaaac tctccctgtg ggccaagcgt
      241 caaaacggtt cggtaaaatg gttctgcgga cagccggtta cgcgcgccga caacgccaaa
      301 gacgacgcca aagacgacgc cgttaccgcc gccgccaccg gcaccgacaa aatcgacacc
      361 aagcacctgc cgtcaacctg ccgcgacgct tcatctgcca gccgtacaaa aacacccggt
      421 ttcgctttca aatacttcca aaaaatcagc cgttaccgag tattgcccga atcacggcaa
      481 atgactaaaa aacttcgtca ttcccgcgca ggcgggaatc taggtttgtc ggcgcagaaa
      541 cttatcgggt aaaaaggttt ctccggtcct gagttctaga ttcccacttt cgtgggaatg
      601 acgggattca atgatgccgc cggcgtggca acctccaccg acat
//
LOCUS       DAXHRI010000293          629 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXHRI010000293 DAXHRI010000000
VERSION     DAXHRI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.293"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..621
                     /locus_tag="ACFM75_002159"
     CDS             <1..621
                     /locus_tag="ACFM75_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6256611.1"
                     /translation="TSTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAADYASYRKWNDN
                     KYSVNTKELQRNKEHGNWIELKTENQGNGSFHAVSSLGLSAIYDFKLNDKFDKFKPYI
                     GARIAYGHVKHQVHSVETETKIIFTTPTKGAKVGGTIPNKSMSKPPYHESNSISSLGL
                     GVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYRF"
BASE COUNT          191 a          171 c          141 g          126 t
ORIGIN      
        1 acaagcacag taagcgatta tttcagaaac atccgtgcgc attccatcca cccccgggtg
       61 tcggtcggct acgacttcgg cggctggaga atagcggcag attatgccag ttacagaaaa
      121 tggaacgaca ataaatattc cgtcaacaca aaagagttgc aaagaaacaa ggagcatggc
      181 aactggatag aactgaagac ggaaaatcag ggaaacggca gcttccacgc cgtctcatcg
      241 ctcggcttat ccgccattta cgatttcaaa ctcaacgata aattcgataa attcaaaccc
      301 tatatcggtg cgcgcatcgc ctacggacac gttaaacatc aggttcattc ggtggaaaca
      361 gaaaccaaga ttattttcac tacaccaacg aaaggcgcta aagttggagg cacgatccca
      421 aacaaatcga tgagcaaacc tccctatcac gaaagcaaca gcatcagcag cttgggtctt
      481 ggtgtcatcg ccggtgtcgg tttcgacatc acgcccaagc tgaccttgga caccggatac
      541 cgttaccaca actggggacg cttggaaaac acccgcttca aaacccacga agtctcattg
      601 ggcgtgcgct accgcttctg attccccga
//
LOCUS       DAXHRI010000294          626 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXHRI010000294 DAXHRI010000000
VERSION     DAXHRI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.294"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(1..235)
                     /locus_tag="ACFM75_002160"
                     /pseudo
     CDS             complement(1..235)
                     /locus_tag="ACFM75_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            342..>626
                     /locus_tag="ACFM75_002161"
     CDS             342..>626
                     /locus_tag="ACFM75_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6256612.1"
                     /translation="MHYKLIELFVAGVTARKAAELVGVNKNTAAYYFHRLRLLIYQNS
                     PHLEMFDGEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVTVP"
BASE COUNT          166 a          142 c          151 g          167 t
ORIGIN      
        1 cactatattc ggtttccaac tgaccgaccc attctgataa gaccgacagg cgttgttcgg
       61 atttcgccac gaacgggttt tcggtatccc acgcgtagcc tgccaaaatc gaagaaagca
      121 tacggctgat ttccgggctg cggtcgggcg cgtacacgac gttttggcag cggaaatcgt
      181 accagccgtc cacatatagt ggattaaatt taaaccggta cagcgttgcc tcgccttagc
      241 tcaaagagaa cgattctcta aggtgctcaa gcaccaagtg aatcggttcc gtgctatttg
      301 tactgcctgc ggcttcgtcg ccttgtcctg atttaaattt aatccactat aaactcattg
      361 aactgtttgt cgcaggcgta actgcaagaa aggcagcaga gttagtaggc gttaataaaa
      421 ataccgcagc ctattatttt catcgtttac gattacttat ttatcaaaac agcccacatt
      481 tagaaatgtt tgatggcgaa gtagaagcag atgaaagtta ttttggcgga caacgcaaag
      541 gcaaacgtgg tcgcggtgct gccggtaaag tcgccgtatt cggtcttttg aagcgaaatg
      601 gtaaggttta tacggttaca gtaccg
//
LOCUS       DAXHRI010000295          608 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXHRI010000295 DAXHRI010000000
VERSION     DAXHRI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.295"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..351
                     /locus_tag="ACFM75_002162"
     CDS             <1..351
                     /locus_tag="ACFM75_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6256613.1"
                     /translation="EYYLNHGEWPANNSSAGVASTATDIKGKYVQSVTVANGVITAQM
                     ASSNVNNEIKGKKLSLWAKRQDGSVKWFCGQPVKRDATATATDDDVTADSGNEKIDTK
                     HLPSTAPTRKSTPN"
     gene            418..608
                     /locus_tag="ACFM75_002163"
                     /pseudo
     CDS             418..608
                     /locus_tag="ACFM75_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          187 a          186 c          137 g           98 t
ORIGIN      
        1 gagtattacc tgaatcacgg cgaatggccc gccaacaaca gttctgccgg cgtggcatcc
       61 accgccaccg acatcaaagg caaatatgtt caaagcgtta cagtcgcaaa cggcgtcatt
      121 accgcccaaa tggcttcaag caacgtaaac aacgaaatca aaggcaaaaa actctccctg
      181 tgggccaagc gtcaagacgg ttcggtaaaa tggttctgcg gacagccggt taagcgcgac
      241 gccaccgcca ccgccaccga cgacgacgtc accgccgaca gcggcaacga aaaaatcgac
      301 accaagcacc tgccgtcaac agcaccgaca agaaaatcga caccaaacta gagaaacctt
      361 tttacccgat aagtttccgt accgacaagg ctggattccc gcctgcgcgg gaatgacgaa
      421 gctatccttt tggccgaagg tcaaaaatca gccgtcaccg agtattacct gaatcacggc
      481 atatggcccg ccaacaacaa ttctgccggc gtggcatcca ccgccaccga catcaaaggc
      541 aaatatgttc aaagcgttga agtcaaaaac ggcgtcgtta ccgccacaat ggcttcaagc
      601 aacgtaaa
//
LOCUS       DAXHRI010000296          599 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXHRI010000296 DAXHRI010000000
VERSION     DAXHRI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.296"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            172..465
                     /locus_tag="ACFM75_002164"
     CDS             172..465
                     /locus_tag="ACFM75_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256614.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          158 a          150 c          160 g          131 t
ORIGIN      
        1 tccattcggc agggaaagcc tgaagaagaa ttttcccgta agatggccgg ctgcctgacc
       61 gacttggaaa actgtttgga aaaatacctc gaacagttcg ggccggtatc ggaaagtatc
      121 gaagcctgta ccgtcaaact gcaggagtaa ccgtcgttct tcaacaggct gatgaaaacc
      181 aacgacaagc tcaataggca gattgacgtg ttgcagaaac agtcggcagc catccacaac
      241 gaagcctata tcgaaatgaa cacgctgctt taccgccacc gcgaagtggt ttccgtccac
      301 aaccgcaagg cggattatgc ggaaaagggc aaagagcgga ttgcgctgtt cccgcgcggc
      361 ttgaacggca tcaccaagct gcctgccgcc gtcctgttgc ccgagcgtcc cgattgtttt
      421 gatatgaagg cagtttggca aatgtttaat agaataccga ggtaggcgta tcggctgccg
      481 gataaaaatg ccgtctgaaa cggaagcgtt cagacggcat tgttgcagtg gaacaaatca
      541 aaatgcaccc cgtcattcct gtctgtacgg tgatagcggc agtgttttcc tgcttcgtt
//
LOCUS       DAXHRI010000297          596 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXHRI010000297 DAXHRI010000000
VERSION     DAXHRI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.297"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..379)
                     /locus_tag="ACFM75_002165"
     CDS             complement(<1..379)
                     /locus_tag="ACFM75_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6256615.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISHAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKF"
     gene            complement(363..>596)
                     /locus_tag="ACFM75_002166"
     CDS             complement(363..>596)
                     /locus_tag="ACFM75_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6256616.1"
                     /translation="VIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSDNS
                     EMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          166 a          117 c          111 g          202 t
ORIGIN      
        1 caaatttgtc ggtttcggtg tcgtattggt agcctcgtgc ctgtatgtct tctttgaaag
       61 tttcgtatac gtcatgggct aaaagggctg ttccgacata gggaaccgcc cttgtgctga
      121 atttcgcgcc taagcgggca agtttgccgc cccccgccaa tacgccggcg tgggaaatgc
      181 tggcggttat tttggcgttg attcgggctt ttgcgcccgt agggatgtgt gttaaatcta
      241 ccgtttttat taaatcagat gaataagttt tactattttt aggtacaaac ttatgaattt
      301 tcgcaccttg tccggtatca actgaaacag tttcagatat ttttactgca tttgcattcg
      361 cttcaaacga atacatcatc aaaattgcaa ttatcgacaa tttcgcaaaa ttcaaatttg
      421 tatattttat gaccatcttt cagggattct ttaattacca tttctgaatt atcagaaaat
      481 gagattagcc aaatatcatg tttaattctt ctattccaga aaaaagagaa acaatcaata
      541 acattttcag acttattaat cttcgcaaat tcaacaaatt cagattgcgc tataac
//
LOCUS       DAXHRI010000298          586 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXHRI010000298 DAXHRI010000000
VERSION     DAXHRI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.298"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>586
                     /locus_tag="ACFM75_002167"
     rRNA            <1..>586
                     /locus_tag="ACFM75_002167"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          169 a          124 c          173 g          120 t
ORIGIN      
        1 ccttttgtat aatgggtcaa cgacttacat tcagtagcga gcttaaccga ataggggagg
       61 cgtagggaaa ccgagtctta atagggcgat gagttgctgg gtgtagaccc gaaaccgagt
      121 gatctatcca tggccaggtt gaaggtgccg taacaggtac tggaggaccg aacccacgca
      181 tgttgcaaaa tgcggggatg agctgtggat aggggtgaaa ggctaaacaa actcggagat
      241 agctggttct ccccgaaaac tatttaggta gtgcctcgag caagacactg atgggggtaa
      301 agcactgtta tggctagggg gttattgcaa cttaccaacc catggcaaac tcagaatacc
      361 atcaagtggt tcctcgggag acagacagcg ggtgctaacg tccgttgtca agagggaaac
      421 aacccagacc gccagctaag gtccccaatg atagattaag tggtaaacga agtgggaagg
      481 cccagacagc caggatgttg gcttagaagc agccatcatt taaagaaagc gtaatagctc
      541 actggtcgag tcgtcctgcg cggaagatgt aacggggctc aaatct
//
LOCUS       DAXHRI010000299          555 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXHRI010000299 DAXHRI010000000
VERSION     DAXHRI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.299"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..85)
                     /locus_tag="ACFM75_002168"
     CDS             complement(<1..85)
                     /locus_tag="ACFM75_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6256617.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAPFFCAG"
     gene            complement(189..515)
                     /locus_tag="ACFM75_002169"
     CDS             complement(189..515)
                     /locus_tag="ACFM75_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256618.1"
                     /translation="MFGFRLSGGTGILQRSQIRRCAVIVRMPIQPKQTSVFRKWMFDG
                     AKVVRRMEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSVYLKQTGKGSNILQSSYSH
                     KRFIVN"
BASE COUNT          147 a          137 c           91 g          180 t
ORIGIN      
        1 ttccagcaca aaaaaacgga gcagttcttt ctgtactttt ttctttaatt tgcagtgcgt
       61 tatcttcata tttcgagggt aacatatctg ctaatctagt acagccccta gtttaaagct
      121 ttgtttctta agtccgcaga gtatgccatg gttagacctt caaagttgag tattgtacta
      181 ttttgttttt aattaactat aaaacgcttg tgtgaatagg aagactgcag aatattgctg
      241 ccttttcctg tttgctttaa ataaacagaa aatgccgtct gaaaccgatt ttcagacggc
      301 atttgtccct taaaacacgt tttttcaagc gcgaccatac caaacaccca caggttgcct
      361 tccatccttc gtacaacctt tgctccatca aacatccact tcctaaatac tgatgtctgc
      421 ttaggttgaa tcggcattcg tacgattacg gcacaccgcc taatctgaga cctttgcaaa
      481 attcctgtcc ctcccgacag ccgaaaccca aacacaggtt ttcggctgtt ttcgccccaa
      541 atacctcctg atttt
//
LOCUS       DAXHRI010000300          546 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXHRI010000300 DAXHRI010000000
VERSION     DAXHRI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.300"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            15..305
                     /locus_tag="ACFM75_002170"
     CDS             15..305
                     /locus_tag="ACFM75_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256619.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGRSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            310..507
                     /locus_tag="ACFM75_002171"
     CDS             310..507
                     /locus_tag="ACFM75_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256620.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
BASE COUNT          168 a          133 c          117 g          128 t
ORIGIN      
        1 aaggaaacca aaaaatgaac atccaacttc aaggccacat cgtcggcgtt aaaaaattca
       61 acggacaaat cgaaggcagg agcttcgact attgccgcct gattgtcgcc acacccttag
      121 acagctccca aggcaacgca ttgggcagct ctactactga atacgatttc ggcggctctg
      181 ccaatttcga gcagttccga aacgcccaat ttccgatcga agcaaacctg aacgtagaaa
      241 tcgttactac gggcaaaacc caaaaactga aagtcatcgg ttttcaactc gtgaagaaag
      301 gctgattgaa tgcagaaagt ctatgttgtc cagtccgtat caacagggga ctttctgtat
      361 ctctctcctg aaacgggcga catcggacat accaaattaa tcaccaatgc cgattatttc
      421 tacgacttcg aagaagcgat taacgcaggt ttggaagaaa tcggcaacca atacgaattt
      481 gtcgtattcg gatttttgaa agactgattt tcggatgttc ggcggtcgtc tgaaaaacgc
      541 tccatc
//
LOCUS       DAXHRI010000301          537 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXHRI010000301 DAXHRI010000000
VERSION     DAXHRI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.301"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(119..391)
                     /locus_tag="ACFM75_002172"
     CDS             complement(119..391)
                     /locus_tag="ACFM75_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256621.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINIIIS
                     YVLIKNILVSVSDEGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
BASE COUNT          177 a          104 c           74 g          182 t
ORIGIN      
        1 tagaacgtat gttgcacacc caatttcaat aggtaaatca gattgcaaat ccagcaattt
       61 gaatattgtc attgttccgt gcaaaaggga tttttattga tgagttgtgt actgggtttc
      121 agcttggctt tttagataat cttttcacaa gaacatacat caatactgtc caaaaaaaaa
      181 gatttgataa atatattgca acatatttta ttccaccttc atccgaaaca gaaaccaata
      241 tatttttaat taatacatag ctgattatta tatttattat tcctataata tataaggatc
      301 ttgccaaaat tttttttgat tttattttag gaatatctct tatccatgct aaaatacagc
      361 ccaatgtcga aaagaaaata acagcaaaca ctaccttacc tcctctcact tccaaccaaa
      421 ccgatagcag gtttggttgg aagttggttt atttattatt taacggcgaa tgtcagtgtt
      481 cttccccgta gaacctgcat aaccatttat gccaccagca acagtcccca ctgctac
//
LOCUS       DAXHRI010000302          526 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXHRI010000302 DAXHRI010000000
VERSION     DAXHRI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.302"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..121)
                     /locus_tag="ACFM75_002173"
     CDS             complement(<1..121)
                     /locus_tag="ACFM75_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6256622.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNR"
BASE COUNT          135 a          102 c          127 g          162 t
ORIGIN      
        1 gacgattcag gtactgttcg atcggctgcc aatcaattac ctgatccaac ttcaatagcg
       61 ggaaacggtc gatgtgtttg gcaatcatgg cttgggcggt ttgctggaag aaggtgctca
      121 tgagaaatct cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg
      181 tctcaaaccg gtttgtcagg gtttaatggt cagcgcgtcc tgcgttgcca tttgttgtag
      241 tagtattcgt aatcttcatc acgaccgccc gattcgatcc aattatcgta ttcggggctg
      301 ttttcaaagc tgtcagacat tatccgtacc tcgcaaaatg ggtttatcga tactttttta
      361 gttatggaat caaccataac taaaaagaag tatacttctt tgcttttccg ccaaaatatt
      421 taaccgcaca tccgctgcag ggtaaaaaat ggaagttcaa cgatattgat acgggtatgg
      481 gcggatgccc ttggattcgg atttcaagtg caacactagt gtatta
//
LOCUS       DAXHRI010000303          525 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXHRI010000303 DAXHRI010000000
VERSION     DAXHRI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.303"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(328..>525)
                     /locus_tag="ACFM75_002174"
     CDS             complement(328..>525)
                     /locus_tag="ACFM75_002174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256623.1"
                     /translation="RFLFLVFCSRGNNEILSFRNLSEKQKSRRRHSRKSGNLETKNYR
                     DLSETTNPLRHHSRKSGNLEI"
BASE COUNT          133 a          120 c          116 g          156 t
ORIGIN      
        1 cgcaggcggg aatccagtct gttgagtttc agtcatttcc gataaatcac cgcaacatta
       61 aatttccaga ttcccgcttt tgcgggaatg acggcggggg gatttctgtt tttccgacaa
      121 attcctaaaa cttaaagttc cgttattccc gcgagaacag aaaaccaaaa acagaaacct
      181 aaaatctcgt cattcccgcg caggcgggaa tccagtccgt tcggtttcgc ttgttttggg
      241 tttcgggtaa cttccacttc gtcattcccg cgaaagtggg aatccggttc gttgagtttc
      301 agtcatttcc gataaatcac cgcaacatta aatttctaga ttcccgcttt tgcgggaatg
      361 atggcggaga gggtttgttg tttcggataa atccctgtag tttttcgttt ctagattccc
      421 gcttttgcgg gaatgacggc ggcgggattt ctgtttttcc gacaaattcc taaaacttaa
      481 aatttcgtta tttccgcgag aacagaaaac caaaaacaga aacct
//
LOCUS       DAXHRI010000304          521 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXHRI010000304 DAXHRI010000000
VERSION     DAXHRI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.304"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>521
                     /gene="mafB"
                     /locus_tag="ACFM75_002175"
     CDS             <1..>521
                     /gene="mafB"
                     /locus_tag="ACFM75_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6256624.1"
                     /translation="EAQLAAATALQDSAFAVKDGIHSAKQWADAHPNITATAQTALSA
                     AEAAGTVWGGKKVELNPTKWDWVKNTGYETTVTRKTLDGQIAGKNKPIKPSSGDYVLP
                     VGRQIIDPTRTSFSQATVSYQKRGANYNYDSLVAAMNEKKSWVGDRVDVVNMPDGAPT
                     SMDNTRIMAAREAG"
BASE COUNT          163 a          113 c          123 g          122 t
ORIGIN      
        1 gaagcacaac ttgcggctgc aaccgcatta caagacagtg cttttgcggt aaaagacggt
       61 atccactctg ccaaacaatg ggctgatgcc catccgaata taactgcaac agcccaaact
      121 gccctttccg cagcagaggc cgcaggtacg gtttggggcg gtaaaaaagt agaacttaac
      181 ccgactaaat gggattgggt taaaaatacc ggctatgaaa caactgttac ccgaaagact
      241 ttagatgggc aaatagctgg caagaacaag cctattaaac cttcttcagg agattatgtc
      301 ttgccggtag gcagacaaat catagatcct actcgtacat ctttttcaca agctacagtt
      361 agctatcaga agcgtggtgc aaattacaac tatgattctc ttgtagctgc aatgaacgaa
      421 aaaaaatcat gggttggcga cagggttgat gttgttaata tgcctgatgg agcacctact
      481 agtatggata acacgcgtat tatggcagca cgtgaagcag g
//
LOCUS       DAXHRI010000305          521 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXHRI010000305 DAXHRI010000000
VERSION     DAXHRI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.305"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>521
                     /locus_tag="ACFM75_002176"
     CDS             <1..>521
                     /locus_tag="ACFM75_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFC6256625.1"
                     /translation="LEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSIS
                     GRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLR
                     GTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDV
                     TGAVTLEEGVEMV"
BASE COUNT          135 a          138 c          142 g          106 t
ORIGIN      
        1 ttggaaggcg atgccgctta tgaagagaaa atcttcgaac tggctgccgc attggacagc
       61 tacatcccga ctcccgagcg tgccgtggac aaaccgttcc tgctgcctat cgaagacgtg
      121 ttctccattt ccggccgagg tacagtagta accggccgtg tagagcgcgg tatcatccac
      181 gttggtgacg agatcgaaat cgtcggtctg aaagaaaccc aaaaaaccac ctgtaccggt
      241 gttgaaatgt tccgcaaact gctggacgaa ggtcaggcgg gcgacaacgt aggcgtattg
      301 ctgcgcggta ccaaacgtga agacgtggaa cgcggtcagg tattggccaa accgggtact
      361 atcactcctc acaccaaatt caaagcagaa gtgtacgtac tgagcaaaga agagggcggc
      421 cgccatactc cgttcttcgc caactaccgt ccgcaattct acttccgtac caccgacgta
      481 accggcgcgg ttactttgga agaaggtgtg gaaatggtaa t
//
LOCUS       DAXHRI010000306          520 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXHRI010000306 DAXHRI010000000
VERSION     DAXHRI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.306"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>520
                     /locus_tag="ACFM75_002177"
     rRNA            <1..>520
                     /locus_tag="ACFM75_002177"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          148 a          103 c          160 g          109 t
ORIGIN      
        1 gtaggccgat gaaggtgcat tgtaaagtgt gctggaggta tcagaagtgc gaatgttgac
       61 atgagtagcg ataaagcggg tgaaaagccc gctcgccgaa agcccaaggt ttcctgcgca
      121 acgttcatcg gcgtagggtg agtcggcccc taaggcgagg cagaaatgcg tagtcgatgg
      181 gaaacaggtt aatattcctg tacttgattc aaatgcgatg tggggacgga gaaggttagg
      241 ttggcaagct gttggaatag cttgtttaag ccggtaggtg gaagacttag gcaaatccgg
      301 gtcttcttaa caccgagaag tgacgacgag tgtctacgga cacgaagtaa ccgataccac
      361 gcttccagga aaagccacta agcttcagtt tgaatcgaac cgtaccgcaa accgacacag
      421 gtgggcagga tgagaattct aaggcgcttg agagaactca ggagaaggaa ctcggcaaat
      481 tgataccgta acttcgggag aaggtatgcc ctctaaggtt
//
LOCUS       DAXHRI010000307          505 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXHRI010000307 DAXHRI010000000
VERSION     DAXHRI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.307"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            166..505
                     /locus_tag="ACFM75_002178"
                     /pseudo
     CDS             166..505
                     /locus_tag="ACFM75_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          156 a          141 c          112 g           96 t
ORIGIN      
        1 aagaaaccgt tttcccgata agtttctgca ccgacaaggc tggattcccg cctgcgcggg
       61 aatgacagcg gagcggtttc tgttgctccc gataaattcc taaaactcaa aatttcatca
      121 ttccctcaaa gacagaaaac caaaataaga aacctaaaat tcgtcattct tttggctgaa
      181 ggtcaaaaat cagccgtcac cgagtattac ctgaatcacg gcatatggcc cggcggcaac
      241 agttctgccg gcgtggcaac ctcctctgaa atcaaaggca aatatgttgc aggggttaaa
      301 gttgaaaacg gcgtcgttac cgccgaaatg aaatcaaccg gcgtaaacaa agaaatccaa
      361 ggcaaaaaac tctccctgtg ggccaagcgt caaaacggtt cggtaaaatg gttctgcgga
      421 cagccggtta cgcgcaacgc caacaacacc gccaacgacg ccgtcaccgc caccgacaag
      481 cagatcgaca ccaagcacct gccgt
//
LOCUS       DAXHRI010000308          493 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXHRI010000308 DAXHRI010000000
VERSION     DAXHRI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.308"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(57..368)
                     /gene="rpsJ"
                     /locus_tag="ACFM75_002179"
     CDS             complement(57..368)
                     /gene="rpsJ"
                     /locus_tag="ACFM75_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC6256626.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(386..>493)
                     /locus_tag="ACFM75_002180"
     CDS             complement(386..>493)
                     /locus_tag="ACFM75_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6256627.1"
                     /translation="TVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT          128 a          118 c          115 g          132 t
ORIGIN      
        1 gcataacata aaaaatcccc aagcattgat tgcttgggga ttttcaatag tccgaattat
       61 tggactttga tttctacatc aacaccggcc ggcaaatcca gcttcatcag cgcatcggta
      121 gttttatcgg tccaatccac gatgtccatc aggcgcaagt gggtgcggat ttccaattgt
      181 tcacgggaag ttttgttcac gtgcggagaa cgcagaatgt tgaaacgttc gattttggtc
      241 ggcaacggaa tcggaccttt tacaacggca ccggtacgtt ttgcagtttc aacgatttct
      301 tgtgcagaac ggtcaatcag ggcgtaatca taagctttca ggcggatacg gattttttgg
      361 tttgccattt atcaatatcc ttcaattaag cgataacaga agaaaccacg ccggcaccca
      421 cggtacgacc gccttcgcga atcgcaaagc gcaggccttc ttccatagcg ataggcgcaa
      481 tcagttctac ggt
//
LOCUS       DAXHRI010000309          492 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXHRI010000309 DAXHRI010000000
VERSION     DAXHRI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.309"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            125..492
                     /locus_tag="ACFM75_002181"
                     /pseudo
     CDS             125..492
                     /locus_tag="ACFM75_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          145 a          140 c          114 g           93 t
ORIGIN      
        1 ttttgaaact taaatcccgt cattcccacg aaagtgggaa tctagaacgt aaaatctcaa
       61 gaaaccgttt tcccgataag tttctgcacc gacagacctg gattcccgcc tgcgcgggaa
      121 tgacgaatcc attcttttgg ccgaaggtca aaaatcagcc gttaccgagt attacctgaa
      181 tcacggcaaa tggcccggcg gcaacagtga tgccggcgtg gcatcttctt caacaatcaa
      241 aggcaaatat gttgagaaag ttgaagtcgc aaaaggcgtc attaccgcca caatgctttc
      301 aaccggcgta aacaaagaaa tccaaggcaa aaaactctcc ctgtgggcca agcgtcaaga
      361 cggttcggta aaatggttct gcggactgcc ggttgcgcgc gacggcaccg acagcgccac
      421 cgacgtcaaa gccgacaccg acgccaccaa caaaatcgac accaagcacc tgccgtcaac
      481 ctgccgcgat ga
//
LOCUS       DAXHRI010000310          471 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXHRI010000310 DAXHRI010000000
VERSION     DAXHRI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.310"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>471
                     /locus_tag="ACFM75_002182"
     CDS             <1..>471
                     /locus_tag="ACFM75_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6256628.1"
                     /translation="RSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQID
                     NAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA
                     DGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFP"
BASE COUNT          131 a          142 c          109 g           89 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggtggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc c
//
LOCUS       DAXHRI010000311          433 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXHRI010000311 DAXHRI010000000
VERSION     DAXHRI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.311"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..183
                     /locus_tag="ACFM75_002183"
     CDS             <1..183
                     /locus_tag="ACFM75_002183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256629.1"
                     /translation="PPPTSTPSTCRQPAAMIHLPFAQEHHAPISNIFKKSAVTEYCPN
                     HGTWPKNFVIPTKVGI"
BASE COUNT          129 a          121 c           84 g           99 t
ORIGIN      
        1 ccaccgccaa catcaacacc aagcacctgc cgtcaacctg ccgcgatgat tcatctgccg
       61 tttgcacaag aacaccacgc gccgatttca aacattttca aaaaatcagc cgttaccgag
      121 tattgcccga atcacggcac atggccgaaa aacttcgtca ttcccacgaa agtgggaatc
      181 tagttcgttg agtttcagtt atttagaata aattttgaaa ctttaatccc gtcattcccg
      241 cgaaagtggg gatccagaac gcaaaatctc aagaaaccgt tttcccgata aatttccgtg
      301 ccgacgaggc tggattcccg cctgcgcggg aatgacgaag ctatccatac ggaaatctac
      361 atcccgtcat tcccacgaaa gtgggaatcc agaacgcaaa atctaaagaa accgttttcc
      421 cgataagttt ctg
//
LOCUS       DAXHRI010000312          415 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXHRI010000312 DAXHRI010000000
VERSION     DAXHRI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.312"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(120..314)
                     /gene="thiS"
                     /locus_tag="ACFM75_002184"
     CDS             complement(120..314)
                     /gene="thiS"
                     /locus_tag="ACFM75_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC6256630.1"
                     /translation="MNIILNGEPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT           89 a           99 c          113 g          114 t
ORIGIN      
        1 aagtgaatcg gttccgtact atccgtactg tctgcggctt cgtcgccttg tcctgattta
       61 aagttaatcc accataacgt tgttttgggg acaggggtcg tctgaaaagg caaaaccgcc
      121 tagccgccga ccaccggccg cacgatatcg attttgtcgt tttcgtttaa aaccgtttcc
      181 gcatacgcgc ctttggggac gaaaacggtg ttgaccgcca cggcaaaggg cttttgcggc
      241 gcggtttggg cgatgaggtc ggcaacgctt gtgccgtgaa gttcggcggg ttcgccgttt
      301 aagatgatgt tcatggtttc tcgatgtttc tgtatttata gtggattaac aaaaaccagt
      361 acggcgttgc ctcgccttag ctcaaagaaa acgattctct aaggtgctga agcac
//
LOCUS       DAXHRI010000313          412 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXHRI010000313 DAXHRI010000000
VERSION     DAXHRI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.313"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>412
                     /locus_tag="ACFM75_002185"
     CDS             <1..>412
                     /locus_tag="ACFM75_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC6256631.1"
                     /translation="QAIIQPQNMDSGILKLRVSAGEIGDIRYEEKRDGKSAEGSISTF
                     NNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKSDLQIKWQQNKPIR
                     FSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFY"
BASE COUNT          137 a           75 c           98 g          102 t
ORIGIN      
        1 caagctatta tccaaccaca gaatatggat tcgggaattc tgaaattacg ggtatcagca
       61 ggcgaaatag gggatatccg ctatgaagaa aaacgggatg ggaagtctgc cgaaggcagt
      121 attagtacat tcaataacaa atttccctta tataggaaca aaattctcaa tcttcgcgat
      181 gtagagcagg gcttggaaaa cctgcgtcgt ttgtcgagtg ttaaaacaga tattcagatt
      241 ataccgtccg aagaagaagg caaaagcgat ttacagatca aatggcagca gaataaaccc
      301 atacggttca gtatcggtat agatgatgcg ggcggcaaaa cgaccggcaa atatcaagga
      361 aatgtcgctt tatcgttcga taaccctttg ggcttaagcg atttgtttta tg
//
LOCUS       DAXHRI010000314          405 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXHRI010000314 DAXHRI010000000
VERSION     DAXHRI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.314"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            112..327
                     /gene="rpmE"
                     /locus_tag="ACFM75_002186"
     CDS             112..327
                     /gene="rpmE"
                     /locus_tag="ACFM75_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC6256632.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          126 a          106 c           74 g           99 t
ORIGIN      
        1 atacatttta tcgaatatcc tcttgacgaa acaacagaaa atagcgagaa tgccgagctt
       61 gtctgaagca taggtacatc ccctatgccc gcttttttga aaggaaatca aatgaaacaa
      121 ggtattcacc cgaactacca cgaagtcaac gttacctgct cttgcggcaa caaattcgca
      181 accaaatccg cgatggaaaa agaaaacttc aatatcgagg tttgctccct gtgccacccg
      241 ttctataccg gcacccaaaa aatcgtcgat accaccggcc gcgtggacaa attcaacaac
      301 aaattcggca acctgttcaa acgctgattg ccgctttgca agaattaagc cctgccatca
      361 tttcgatggc agggcttttt aatatttggc acactgactt cttat
//
LOCUS       DAXHRI010000315          399 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXHRI010000315 DAXHRI010000000
VERSION     DAXHRI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.315"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           77 a           84 c          121 g          117 t
ORIGIN      
        1 ggcgaaccaa cgctgtactg gtttagattt aattcactat acctgcgtaa tggtatcggg
       61 tactcatcat caacctgcct tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc
      121 gatgggttgc cctgccgctc cctgtgttgc gcaacggttg tgtgccttgg gtgggcgcgg
      181 gtggcggccg ggcggtttgt tgctatgata cgggagttcc gatatacaag ggtgggcttc
      241 agcccaccaa tcaacgaccg aaaaagaaaa aacgttgggc tgaagcccac tctacgcaat
      301 tacgcaactg ttatcgtggg gatgacggga ttttaggttt ttgtttttct gtttttgcgg
      361 gaaggatggg ttttaggttg cggtcattta tcggaaaag
//
LOCUS       DAXHRI010000316          394 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXHRI010000316 DAXHRI010000000
VERSION     DAXHRI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.316"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..260
                     /locus_tag="ACFM75_002187"
     CDS             <1..260
                     /locus_tag="ACFM75_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6256633.1"
                     /translation="RQLLDGIMRKTCRNRPLTETQTKRNRYLSKTRYVVEQSFGTLHR
                     KFRYARAAYFRLIKVSAQSHLKAMCLNLLKAANRLSVPVAA"
BASE COUNT          102 a           83 c          117 g           92 t
ORIGIN      
        1 agcgtcagtt gttagacggt attatgcgca aaacctgccg caaccgtccg ctgacggaaa
       61 cgcaaaccaa acgcaaccga tatttgtcga agacccgtta tgtggtcgaa cagagcttcg
      121 gtacgttgca ccgtaagttc cgctatgcgc gggcagccta tttcagactg attaaagtga
      181 gtgcgcaaag ccacctgaag gcgatgtgtt tgaacctttt gaaagccgcc aacaggctaa
      241 gtgtgcctgt tgccgcctaa aaagcagccc ggatgcctga ttatcgggtg tccggtgagg
      301 attaaggggg tatttgggta gaattaggag gtatttgggg cgaaaataga cgaaaacctg
      361 tgtttggatt tcggctgtcg tgagggaaag gaat
//
LOCUS       DAXHRI010000317          389 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXHRI010000317 DAXHRI010000000
VERSION     DAXHRI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.317"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(188..>389)
                     /locus_tag="ACFM75_002188"
     CDS             complement(188..>389)
                     /locus_tag="ACFM75_002188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256634.1"
                     /translation="TIMQNHDKTGARLHKRMKSTDIQTQSFLAHFQRIVNAENFVNRF
                     FAQASASFRGMGNRIRKRTHAT"
BASE COUNT          106 a           80 c           76 g          127 t
ORIGIN      
        1 tcggtctgca acaaggtcgt ctgacaattc tcaaaacggc tgaggttgtg aatttgggca
       61 accaatactt tctttgccaa aaaaatattt tctttcaaac tcaacttgta tttgtcggga
      121 aacacgataa ttccttaaat aattaaagca gtacgttaaa tcatcatctt atcatcactt
      181 tcggggttta tgttgcatgc gtccgttttc taatacggtt tcccatcccg cgaaacgagg
      241 cggaggcctg tgcaaaaaac ctgttcacga aattttccgc attaacgata cgctgaaaat
      301 gcgctaaaaa tgactgtgtt tgaatatcgg ttgatttcat ccgtttgtgt aacctcgcgc
      361 cggttttgtc atgattttgc attatagtg
//
LOCUS       DAXHRI010000318          382 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXHRI010000318 DAXHRI010000000
VERSION     DAXHRI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.318"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            123..302
                     /locus_tag="ACFM75_002189"
     CDS             123..302
                     /locus_tag="ACFM75_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256635.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
BASE COUNT           98 a           71 c          102 g          111 t
ORIGIN      
        1 atttgtactg tcttcggctc gccgccttgt cctgatttaa atttaattca ctatatttgc
       61 ccgtgcgttt gtgataaatt cggcactgta tttgatatga ttttaacaaa tcgggagggg
      121 caatgttttg gtatgtgatt ggcttttgcg cctttgtcgt cgcgctgctg tcgctgtggg
      181 tcaatgccgg cgcgttcggt atgcaggaag acgatactcc gcaatcggat tatgaaaggc
      241 gtttgggctt gggtgcgaag ctgaaaaaca aaaatccgga tacggacgaa aaacggcaat
      301 aaagacaaca aatgccgtct gaaaggtttt cagacggcat ttgctttgga gcggatatag
      361 tggattaaca aaaatcagga ca
//
LOCUS       DAXHRI010000319          382 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXHRI010000319 DAXHRI010000000
VERSION     DAXHRI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.319"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>382
                     /locus_tag="ACFM75_002190"
     CDS             <1..>382
                     /locus_tag="ACFM75_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256636.1"
                     /translation="FKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYS
                     AAETKEAQLALLDNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKK
                     NALVSLSDKAKAAIDAARDRIAVLD"
BASE COUNT          111 a           95 c           91 g           85 t
ORIGIN      
        1 tttaaagata aagtggaact cactgccgaa caggcaaaag ccgccaatct tgcgggcatc
       61 ggccgtctgc gcgatttgcg cgaagctgcc gcattgtccg gcgatttggc caatatgctg
      121 aaagcttatt ctgccgccga aactaaagaa gcacagttgg cattgttaga taatttgatt
      181 cacaaatggg cggaaaccga ttcgaactgg ggcaaaaaat cgccaatgcg actttcaacc
      241 gattggacgc aaacggctaa tgaaggtatt gcactgacac catcccaagt agcacaacta
      301 aaaaagaacg ctttagtttc cctttctgat aaagctaaag cagctattga cgccgcccgc
      361 gaccgcattg ccgtgcttga tg
//
LOCUS       DAXHRI010000320          375 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXHRI010000320 DAXHRI010000000
VERSION     DAXHRI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.320"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>375
                     /locus_tag="ACFM75_002191"
     CDS             <1..>375
                     /locus_tag="ACFM75_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC6256637.1"
                     /translation="PEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARCGLL
                     FATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAEYRA
                     QNGAFKSVDDLTKVKGIGPAVLA"
BASE COUNT           80 a           86 c          118 g           91 t
ORIGIN      
        1 cccggaaaaa atgtcgggga tggcgggacg gcatccgtac ggcgtccggt cgggtttgcg
       61 gaggaacggc ttgaaacttt gggatattca ttttagaatg actcgtttta tcgtcgcaag
      121 atgcggttta ttgtttgcaa cccttaaagg aaaaaccatg aagaaaatgt tcgtgctgtt
      181 ctgtatgctg ttctcctgcg ccttctccct tgcggcggta aacatcaatg cggcttcgct
      241 gcaggagttg gaggcgctgc cgggcatagg ccctgcgaag gcgaaggcca ttgcggaata
      301 ccgtgcgcaa aacggtgcgt tcaagtctgt ggacgatttg accaaggtaa agggcatcgg
      361 ccctgcggtg ctggc
//
LOCUS       DAXHRI010000321          368 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXHRI010000321 DAXHRI010000000
VERSION     DAXHRI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.321"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           78 a           86 c          113 g           91 t
ORIGIN      
        1 gcgcaggcgg gaatccagtc cgcttgttcg gtaaatgaga gggcggattg cgcgcctgtc
       61 agataaaccc cgtgtttaaa cgggcggcaa tgaggtacgc gcagagcctt gaagcgcaat
      121 cgatatatta ttttcagcca aaacggacgc ccccgcttgc cttgcaaacc cttaaaaagg
      181 aagccacccg gattaatccg agtggccgtg gaaaatcact taccgcttga tttatttaaa
      241 atttatggta taatttgcct tagctggcat cacttgcgtc gcggcaggtt gacggcaggt
      301 gcttggtgtt gatgttggtg gtggtgtcgg cggtaacggc gtcggcgtcg gcgtcggcgt
      361 cggctttg
//
LOCUS       DAXHRI010000322          364 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXHRI010000322 DAXHRI010000000
VERSION     DAXHRI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.322"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..283)
                     /locus_tag="ACFM75_002192"
     CDS             complement(<1..283)
                     /locus_tag="ACFM75_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6256638.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDTPSSAV
                     YNNPYGATPYSPAPASDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYIPS"
BASE COUNT           97 a           89 c          108 g           70 t
ORIGIN      
        1 aagaaggaat gtatgtaccc gaaacggcag gtgcagacgg cggaacataa gcaggaggcg
       61 tataaaccgg cgcgctttgc accggcggca cataaggcgc atcgctggca ggagccgggc
      121 tgtacggcgt tgctccatag gggttgttgt aaactgccga agacggcgtg tcctgcatac
      181 ctgaattgcc tgcaatgacg ggggcaggct gctgggtagc acaaccgccc aacagtgcgg
      241 caaaggaggc gcaagccgcc aaaagtgtcg tttgtttcaa cataagataa ccttcatgtt
      301 ccgatatata gcctgaatgc ggtatatcat aataaaaatg cgcgttcttc tcaagcgcaa
      361 agcc
//
LOCUS       DAXHRI010000323          355 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXHRI010000323 DAXHRI010000000
VERSION     DAXHRI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.323"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..107)
                     /locus_tag="ACFM75_002193"
     CDS             complement(<1..107)
                     /locus_tag="ACFM75_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6256639.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDRVIDWQPIE"
     gene            complement(104..>355)
                     /locus_tag="ACFM75_002194"
     CDS             complement(104..>355)
                     /locus_tag="ACFM75_002194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256640.1"
                     /translation="PDVIVVEDNCPLFGIFLKSGLCPEYCSYKSNISLRLTLEYIGIP
                     FLLADEFGLIVLKPICKLRPLQNSPKSPKFPPRHLGDFS"
BASE COUNT          106 a           62 c           85 g          102 t
ORIGIN      
        1 tgctcgatcg gctgccaatc aattacccgg tccaacttca atagtgggaa ccggtcgatg
       61 tgtttggcaa tcatggcttg ggcggtttgc tggaagaagg tgctcatgag aaatccccta
      121 aatgtcttgg tgggaattta ggggattttg gggaattttg caaaggtctc aacttacaaa
      181 ttggttttaa aacgatcaga ccaaattcat ctgccagcaa aaatggaatg ccgatatatt
      241 ctaacgtaag tcgaagagaa atatttgatt tgtatgaaca atattcaggg cagaggcctg
      301 attttaggaa tattccgaat aaagggcaat tatcttctac gactattaca tcagg
//
LOCUS       DAXHRI010000324          352 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXHRI010000324 DAXHRI010000000
VERSION     DAXHRI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.324"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..243
                     /locus_tag="ACFM75_002195"
     CDS             <1..243
                     /locus_tag="ACFM75_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256641.1"
                     /translation="QIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPDGSPAKAVVLK
                     ANQNGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            246..>352
                     /locus_tag="ACFM75_002196"
     CDS             246..>352
                     /locus_tag="ACFM75_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6256642.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESID"
BASE COUNT          123 a           61 c           79 g           89 t
ORIGIN      
        1 cagattagga acggtacggt acagctgaat ccgaatggtg cgggtggata tacgcagatg
       61 agtcgtgaat ggattaaaca agttgtaaaa agtttacctg atggtagtcc tgctaaggca
      121 gttgtcttaa aagcaaatca gaacggcaaa ttaaaaacgg caatagcagg cgttgatcgt
      181 caaacaggta aggccgttat tctttctgtc aaagttcctt ctaaaaccaa tataaggaga
      241 taacaatggg gcacaatatg atgaccaccc aaaaatggta tgaacatatt actaatgtaa
      301 tcataggcaa tactgctaat ttcaatagcg gttgccccga atctatagat ta
//
LOCUS       DAXHRI010000325          349 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXHRI010000325 DAXHRI010000000
VERSION     DAXHRI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.325"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(<1..>349)
                     /locus_tag="ACFM75_002197"
     CDS             complement(<1..>349)
                     /locus_tag="ACFM75_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256643.1"
                     /translation="VKEYQPPVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     DVVPVFGALIRDADFQYIFLSYNNEGLMSVGQVREIFERFGKYNLVQTEYRRFKADKT
                     ENRNHKANSIFEYL"
BASE COUNT          118 a           86 c           57 g           88 t
ORIGIN      
        1 cagatattcg aatatcgaat ttgccttatg attacggttt tctgtcttat ctgccttaaa
       61 acgccgatat tccgtttgaa ccaaattata tttgccgaaa cgctcaaaaa tctcccttac
      121 ctgcccgaca gacatcaagc cttcgttgtt gtagctgaga aaaatatatt gaaagtccgc
      181 atccctaatc aaagccccaa ataccggaac cacatccgat tttgagcaag tccgcgctaa
      241 aacacatcgt acagcgcaaa accggatggg aatggcaaaa tccggaaaca aacatttttc
      301 aaagcataca aacaaaaaaa taccaaccgg aggttggtat tccttaact
//
LOCUS       DAXHRI010000326          341 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXHRI010000326 DAXHRI010000000
VERSION     DAXHRI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.326"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..318
                     /locus_tag="ACFM75_002198"
     CDS             <1..318
                     /locus_tag="ACFM75_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6256644.1"
                     /translation="DTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFYGENHR
                     VDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANTQSVQQPLLVT
                     PSA"
BASE COUNT           94 a           67 c           74 g          106 t
ORIGIN      
        1 gacactatgc actttaaagg atttaaagca gcagatgttc attttatccg ttccggaagt
       61 gatttggtgc ttagcgcttc tgaacaagac aacgtacgta tttccggatt cttctatggt
      121 gaaaaccatc gtgtagatac atttgtcttt gatgatgcag ctatcagtaa tccagatttt
      181 gccaagtata ttaatgctgg caataatttg gtacagtcta tgtctgtgtt cggttctaat
      241 actgctgcga caggaggaaa tgtggatgcc aatacacaat ccgtacagca gccgttattg
      301 gtaacgccat ctgcataagg agcctaatca cattcatggc t
//
LOCUS       DAXHRI010000327          340 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXHRI010000327 DAXHRI010000000
VERSION     DAXHRI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.327"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT          111 a           92 c           66 g           71 t
ORIGIN      
        1 cgaaagtggg aatccagaac gcaaaatctc aagaaaccgt tttcccgata agtttccgta
       61 ccgacagacc tagattcccg cctgcgcggg aatgacggcg gagcggtttc tgttgctccc
      121 gataaattcc taaaactcaa aatttcatca ttccctcaaa aacagaaaac caaaaacagc
      181 aacctaaaat tcatcattcc cacgaaagtg ggaatccaga acgcaaaatc taaagaaacc
      241 gttttacccg ataagtttcc gtaccgacag acctggattc ccgcctgcgc gggaatgacg
      301 aagctatcct tttggctgaa ggtcaaaaat cagccgtcac
//
LOCUS       DAXHRI010000328          337 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.328, whole genome shotgun sequence.
ACCESSION   DAXHRI010000328 DAXHRI010000000
VERSION     DAXHRI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.328"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>337
                     /gene="mafA"
                     /locus_tag="ACFM75_002199"
     CDS             <1..>337
                     /gene="mafA"
                     /locus_tag="ACFM75_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6256645.1"
                     /translation="GRKGERSAGLSVNGMGDYRNETLLANPRDVSFLTNLIQTVFYLR
                     GIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLI
                     APKTAAYES"
BASE COUNT           81 a          131 c           72 g           53 t
ORIGIN      
        1 gcggacgcaa aggcgaacgc tccgccggac tgtccgtcaa cggcatgggc gactaccgca
       61 acgaaaccct gctcgccaac ccccgcgacg tttccttcct gaccaacctc atccaaaccg
      121 tcttctacct gcgcggcatc gaagtcgtac cgcccgaata cgccgacacc gacgtattcg
      181 taaccgtcga cgtattcggc accgtccgca gccgtaccga actgcacctc tacaacgccg
      241 aaacccttaa agcccaaacc aagctcgaat atttcgccgt cgaccgcgac agccggaaac
      301 tgctgattgc ccctaaaacc gccgcctacg aatccca
//
LOCUS       DAXHRI010000329          336 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.329, whole genome shotgun sequence.
ACCESSION   DAXHRI010000329 DAXHRI010000000
VERSION     DAXHRI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.329"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>336
                     /locus_tag="ACFM75_002200"
     rRNA            <1..>336
                     /locus_tag="ACFM75_002200"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           79 c           92 g           78 t
ORIGIN      
        1 ggctgcgact gtttattaaa aacacagcac tctgctaaca cgaaagtgga cgtatagggt
       61 gtgacgcctg cccggtgctg gaaggttaat tgaagatgtg agagcatcgg atcgaagccc
      121 cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag
      181 ttccgacccg cacgaatggc gtaacgatgg ccacactgtc tcctcctgag actcagcgaa
      241 gttgaagtgg ttgtgaagat gcaatctacc cgctgctaga cggaaagacc ccgtgaacct
      301 ttactgtagc tttgcattgg actttgaagt cacttg
//
LOCUS       DAXHRI010000330          336 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.330, whole genome shotgun sequence.
ACCESSION   DAXHRI010000330 DAXHRI010000000
VERSION     DAXHRI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.330"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           91 a           83 c           68 g           94 t
ORIGIN      
        1 taaaaaattt atgctattaa atcagtaatt tctgatgaat tttgaaaact taatcccgtc
       61 attcccgcgc aagcgggaat ccggctcgtt cggtttcgct tgttttaagt ttcgggtaac
      121 ttccacttcg tcattcccgc gcaggcggga atccggttca ttgaatttca gctatttaga
      181 ataaattttg aaactctaat cgcgtcattc ccacgaaagt gggaatccag gacgcaaaat
      241 ctcaagaaac cgttttaccc gataagtttc cgcaccgaca gacctggatt cccgcctgcg
      301 cgggaatgac gaatccatcc gtacggaaac ctgcat
//
LOCUS       DAXHRI010000331          330 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.331, whole genome shotgun sequence.
ACCESSION   DAXHRI010000331 DAXHRI010000000
VERSION     DAXHRI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.331"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(126..>330)
                     /locus_tag="ACFM75_002201"
     CDS             complement(126..>330)
                     /locus_tag="ACFM75_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256646.1"
                     /translation="IGGQSTAAIADLAGEMRRLAKKSKMLLKGLWPFLSSTISAFKIN
                     QSLFKHYCKTVQLLNCKHRKKNY"
BASE COUNT          102 a           58 c           65 g          105 t
ORIGIN      
        1 cgaaaaaacg cgacgtaacc agcatcaaca tatataagaa cagcacaaat agcatcaata
       61 catcaggcaa cgaaaatgca gaataatgca cttaatggtg tttggatatc tgttgttttg
      121 tgctgttagt aattcttctt tctgtgttta cagtttagca gttgtacagt tttacagtaa
      181 tgtttaaaca atgactgatt tattttaaat gcagatattg tagaggataa aaatggccaa
      241 agtcctttca gtaacatttt tgatttttta gcgagccttc tcatttcccc ggcgagatcg
      301 gcaatggcag cggtactttg gccgccgata
//
LOCUS       DAXHRI010000332          328 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.332, whole genome shotgun sequence.
ACCESSION   DAXHRI010000332 DAXHRI010000000
VERSION     DAXHRI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.332"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT          104 a           92 c           77 g           55 t
ORIGIN      
        1 ttcgcgggaa tgacggcatc agtttgcagg attcggcgga aacggtaaaa acggcagaat
       61 cgatgggaat cggcaggctg aagcccaccc taccattatt tacacatccg taccgcttaa
      121 atgccgtctg aaacttcgtc attcccgtga aaacgggaat ccagcctcgt gggagcagaa
      181 acttacaccc cgtcattccc gcgaacgcgg gaatccagta accgaaaaac cacaggaatc
      241 tatcggaaaa acagaaaccc tcgaccgtca ttcccgcgaa cgcgggaatc cagtaaccga
      301 aaaaccacgg gaatctatcg gaaaaaac
//
LOCUS       DAXHRI010000333          323 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.333, whole genome shotgun sequence.
ACCESSION   DAXHRI010000333 DAXHRI010000000
VERSION     DAXHRI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.333"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>323
                     /locus_tag="ACFM75_002202"
     CDS             <1..>323
                     /locus_tag="ACFM75_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6256647.1"
                     /translation="LELINRQLTEKGLKIEKASAAVVDVTIIQTADSKQRQAIEVDEE
                     GQINGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDAEGYIGKLHITPANAHECKHLSP
                     LLEGIA"
BASE COUNT          110 a           85 c           73 g           55 t
ORIGIN      
        1 ctggaactga ttaaccgcca actgaccgaa aaaggtttaa aaatagagaa agcatccgcc
       61 gccgtcgttg atgtcaccat tattcagacc gccgacagca aacagcgtca ggccatagaa
      121 gtcgatgaag aaggacaaat caacggccaa accacaccga gtaaggacaa agatgcccgt
      181 tggatcaaga aaaacggtct ctacaaactc ggttacaaac aacatacccg taccgatgca
      241 gaaggctata tcgggaaact gcacattacc cccgccaatg ctcatgagtg caaacacctg
      301 tcgccgttgt tggaagggat agc
//
LOCUS       DAXHRI010000334          313 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.334, whole genome shotgun sequence.
ACCESSION   DAXHRI010000334 DAXHRI010000000
VERSION     DAXHRI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.334"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           76 a           88 c           77 g           72 t
ORIGIN      
        1 atggcggtat aaagcgcggt atggcggaaa acctgccgtt tccaagtttt attcatcttt
       61 tattccttga gtttgccttc acgggacggg gcggcgcgcc gcgaacgccg ggcttcggta
      121 aaccgcccga ttccgcgccc gccgaattgc tgattgaaaa gcttacctcc ccattttaac
      181 tttgcacact gatcacggta aagcgttttt tagcaaaccg ctgcagatgc ccgacagtct
      241 ggattcccgc ctacgcggga atgacggttt agaagttgcc cgaaacctca aaaaaaaccg
      301 aaaccgaaca agc
//
LOCUS       DAXHRI010000335          305 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.335, whole genome shotgun sequence.
ACCESSION   DAXHRI010000335 DAXHRI010000000
VERSION     DAXHRI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.335"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            63..305
                     /locus_tag="ACFM75_002203"
                     /pseudo
     CDS             63..305
                     /locus_tag="ACFM75_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT           82 a           85 c           76 g           62 t
ORIGIN      
        1 cggtttcttg agattttgcg ttctagattc ccactttcgt gggaatgacg ggatttaatg
       61 atgccgccgg cgtggcatcc tccccctccg acatcaaagg caaatatgtt gagaaagttg
      121 aagtcgcaaa cggcgtcatt accgcccaaa tggcttcaag caacgtaaac aacgaaatca
      181 aaggcaaaaa actctctctg tgggccaagc gtcaagacgg ttcggtaaaa tggttctgcg
      241 gacagccggt tacgcgcgac gcccccaacg ccagcgccga cgccgttaac aaagtcaccg
      301 gcaac
//
LOCUS       DAXHRI010000336          303 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.336, whole genome shotgun sequence.
ACCESSION   DAXHRI010000336 DAXHRI010000000
VERSION     DAXHRI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.336"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(108..302)
                     /locus_tag="ACFM75_002204"
     CDS             complement(108..302)
                     /locus_tag="ACFM75_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256648.1"
                     /translation="MSHSKLWANVACAAGTVKFVMLPDPSAEIWAVYLGIVGGYAVAR
                     SLVSVKRQEVENESRETAGE"
BASE COUNT           70 a          103 c           73 g           57 t
ORIGIN      
        1 tcttgtcaat gaccgcctgc tgcttcgcaa aagccgactt gtagccttga tggtgcgaca
       61 ccgccaagag cgtgccgaca agcgcgatga tggcaatcgg ttgccagtta ttcgccagca
      121 gtttcacgag attcattctc gacctcctga cgtttgacgc tgaccaacga acgcgccacc
      181 gcatagccgc cgacaatgcc caaatacacc gcccaaatct ccgccgacgg gtcgggcagc
      241 atcacaaact taaccgtccc cgccgcgcag gcaacgtttg cccacagttt cgagtgcgac
      301 aca
//
LOCUS       DAXHRI010000337          298 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.337, whole genome shotgun sequence.
ACCESSION   DAXHRI010000337 DAXHRI010000000
VERSION     DAXHRI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.337"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           76 a           79 c           71 g           72 t
ORIGIN      
        1 cgtatgctac cgcatactgg cctttttctg ttagggaaag ttgcacttca aatgcgaatc
       61 cgccaacctt tgaatattgc cgccgtcccg cgttctagat tcccgcctgc gcgggaatga
      121 cgattcatca gtttcccgaa acgcccgaaa aaccgaaacc gagcaaaccg gattcccgcc
      181 tgcgcgggaa tgacgattta taagtttccc gaaacgccca aaaaaaccga aatccgacag
      241 gcgggatttt tgctttcgtg gggatgacgg gattttaagt tacggtcatt tatcggaa
//
LOCUS       DAXHRI010000338          296 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.338, whole genome shotgun sequence.
ACCESSION   DAXHRI010000338 DAXHRI010000000
VERSION     DAXHRI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.338"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            48..260
                     /locus_tag="ACFM75_002205"
     CDS             48..260
                     /locus_tag="ACFM75_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256649.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
BASE COUNT          105 a           28 c           63 g          100 t
ORIGIN      
        1 caggcaaacg gtattttgat gacgaattaa aataaaagga attattcatg aaattaatta
       61 attttgaaga gagttcagtt aaaattagtc tcgataaaga tgagttgtat attattcaag
      121 caattgtagg agaaatttat tcaggcgtat gcgtggattg tagagatttt gagataattc
      181 atggtgttga aaaaataaag ttctttcttt ggataaagaa cttaaaaaaa tatatgatac
      241 ttgggataaa tgttagctag gtaaggtggg cacttgttgc ccaccttttg ccgtat
//
LOCUS       DAXHRI010000339          274 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.339, whole genome shotgun sequence.
ACCESSION   DAXHRI010000339 DAXHRI010000000
VERSION     DAXHRI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.339"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            complement(17..>274)
                     /locus_tag="ACFM75_002206"
     CDS             complement(17..>274)
                     /locus_tag="ACFM75_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm8 family immunity protein"
                     /protein_id="HFC6256650.1"
                     /translation="IGIEGEQGSSIFSLCICSPKWISHHCNKEGFFWSNALILEQFDH
                     KIIKSEIDKILEYCSKETWDLTLSNLLRFFSWEFEDYNPNT"
BASE COUNT           97 a           45 c           36 g           96 t
ORIGIN      
        1 tcatcccaaa ttgctcttat gtgtttggat tgtaatcttc gaattcccaa gaaaaaaatc
       61 gtaataagtt tgaaagtgtc aaatcccaag tttcttttga gcaatattct aatattttat
      121 caatttcact tttaataatc ttatgatcaa actgttctaa tattaatgca ttagaccaaa
      181 aaaaaccttc tttattacaa tgatgggaaa tccatttagg agaacaaatg caaagtgaaa
      241 aaatagatga gccttgttct ccttcgattc cgat
//
LOCUS       DAXHRI010000340          251 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.340, whole genome shotgun sequence.
ACCESSION   DAXHRI010000340 DAXHRI010000000
VERSION     DAXHRI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.340"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>251
                     /locus_tag="ACFM75_002207"
     CDS             <1..>251
                     /locus_tag="ACFM75_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6256651.1"
                     /translation="VRGKGVQVNAKNLSIQSVQDSETYQSKQQNASAQVTVGYGFSAS
                     GDYSQSKIRANHASVTEQSGIYAGEDGYQIKVGNHTDLKG"
BASE COUNT           91 a           61 c           58 g           41 t
ORIGIN      
        1 gtacgcggca aaggcgtaca agtcaatgcc aaaaacctaa gtattcaaag cgtacaagat
       61 agcgaaacct atcaaagcaa acaacaaaac gccagtgcac aagtgaccgt cggttatggc
      121 ttcagtgcca gtggcgatta cagccaaagc aaaatccgag ccaatcatgc ttcggtaacc
      181 gagcaaagcg gtatttatgc cggagaagac ggctatcaaa tcaaggtcgg aaaccataca
      241 gacctcaaag g
//
LOCUS       DAXHRI010000341          250 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.341, whole genome shotgun sequence.
ACCESSION   DAXHRI010000341 DAXHRI010000000
VERSION     DAXHRI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.341"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           82 a           59 c           57 g           52 t
ORIGIN      
        1 tggatggatt cgtcgttccc gcgaaagcgg gaatccggaa ccagaaacca caaaaattta
       61 tcgggaaaac cgaaactcaa aaacaacgga aaccgaacgg acaggattcc cgcctgcgcg
      121 ggaatgacgg ctcatacatc acccaaaaaa aattgaaacc aaacagatgg gattccttct
      181 tgcgtgaggc tgacagatgc cgtctgaaag actttcagac ggcatagctt tttctctttg
      241 aatttatagt
//
LOCUS       DAXHRI010000342          247 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.342, whole genome shotgun sequence.
ACCESSION   DAXHRI010000342 DAXHRI010000000
VERSION     DAXHRI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.342"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           85 a           54 c           66 g           42 t
ORIGIN      
        1 actcaaagct gcaagaattt atcagaaaca actggaactt aaaaaactag attcccgcct
       61 gcgcgggaat gacgaagggt taaagaaatg acgagaaacg gcggtaattt atcgggaata
      121 accgaaaacg aacggaaatc aggacaaggc ggcgaagccg cagacagtac ggatagtgcg
      181 gaaccgactc actcggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag
      241 gcaacgc
//
LOCUS       DAXHRI010000343          239 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.343, whole genome shotgun sequence.
ACCESSION   DAXHRI010000343 DAXHRI010000000
VERSION     DAXHRI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.343"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            <1..>239
                     /gene="mafA"
                     /locus_tag="ACFM75_002208"
     CDS             <1..>239
                     /gene="mafA"
                     /locus_tag="ACFM75_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6256652.1"
                     /translation="VLTACGTLTGIPAHGGGKRFAVEQELVAASSRAAVKEMDLSALK
                     GRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYH"
BASE COUNT           52 a           84 c           64 g           39 t
ORIGIN      
        1 cgtcctaacc gcctgcggca cactgaccgg catacccgcc cacggcggcg gcaaacgctt
       61 tgccgtcgaa caagaactcg tcgccgcatc gtcccgcgcc gccgtcaaag aaatggattt
      121 gtccgccctg aaaggacgca aagccgccct ttacgtctcc gttatgggcg accaaggttc
      181 gggcaacata agcggcggac gctactctat cgacgcactg atacgcggtg gctaccaca
//
LOCUS       DAXHRI010000344          235 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.344, whole genome shotgun sequence.
ACCESSION   DAXHRI010000344 DAXHRI010000000
VERSION     DAXHRI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.344"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           81 a           52 c           51 g           51 t
ORIGIN      
        1 attgaagatg gctgaaattc aaagttacag aaatttataa acaaaaaaca gcaacccgcc
       61 gccgtcattc ccgcgaaagc gggaatccag tcccttgacc ttaagataat ctttaattcg
      121 tcattcccgc aaaagcgaga atccggaagc gaaaagtggt gggaatgtat tagaaatgac
      181 tgaaactcaa aaaaccggat tcccgcctgc gcgggaatga cgattgatac gtttc
//
LOCUS       DAXHRI010000345          234 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.345, whole genome shotgun sequence.
ACCESSION   DAXHRI010000345 DAXHRI010000000
VERSION     DAXHRI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.345"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           75 a           51 c           45 g           63 t
ORIGIN      
        1 ttgctagacg gctgatattt ataaagtgcc aactgcgcct gagtgataaa cggcttgttc
       61 atggttctgc ctttcaatga ttgttttgaa agcctgattt tgacaccata acttcatgcg
      121 ctcaattctt aaacagaacc gccccgatta atacgggtac ggaaacgccg agataaaaat
      181 aaaaatccat catttcaaaa cctttttcag cagggaaaca aagtaaacgg acgc
//
LOCUS       DAXHRI010000346          234 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.346, whole genome shotgun sequence.
ACCESSION   DAXHRI010000346 DAXHRI010000000
VERSION     DAXHRI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.346"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            51..>234
                     /locus_tag="ACFM75_002209"
     CDS             51..>234
                     /locus_tag="ACFM75_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256653.1"
                     /translation="MSSEHVFGRRFVGGYRSGTSEKGFQAVFGRVVTVENVIRLPEKF
                     GRFSDSLWYWAQQTQVQ"
BASE COUNT           61 a           40 c           75 g           58 t
ORIGIN      
        1 tttgtccgcc ttaaagcagg taaacaccgc aatgaatcga cgcaaagaaa atgtcgtctg
       61 aacatgtgtt cgggcggcgt tttgttgggg ggtatcggag tggaacgtct gaaaaagggt
      121 ttcaggcggt ctttgggcgt gtggtgacag tcgaaaacgt gataaggcta cctgaaaagt
      181 ttgggagatt ttcagatagc ctttggtatt gggcgcaaca gacgcaggta caga
//
LOCUS       DAXHRI010000347          230 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.347, whole genome shotgun sequence.
ACCESSION   DAXHRI010000347 DAXHRI010000000
VERSION     DAXHRI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.347"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
     gene            40..>230
                     /locus_tag="ACFM75_002210"
     CDS             40..>230
                     /locus_tag="ACFM75_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6256654.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNL"
BASE COUNT           73 a           44 c           61 g           52 t
ORIGIN      
        1 gtgttttcca tcgttaagag agtgatgaag taagacggca tgtactacca agtcggaaat
       61 aaatgtcttg agaagcacca ggctgaaaac ctttatttca gcttggtagt accaagaatc
      121 aaagaaaacg gacagattgt caggccggaa tataacggca gcctgtggaa gatgtcggac
      181 ggtcagccgc taaggctttt attggcggaa tgcagtccga aagacaacct
//
LOCUS       DAXHRI010000348          229 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.348, whole genome shotgun sequence.
ACCESSION   DAXHRI010000348 DAXHRI010000000
VERSION     DAXHRI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.348"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           60 a           62 c           42 g           65 t
ORIGIN      
        1 accttagaga atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa
       61 tccactatat ttttgcccga cggggtgaaa aatacagttg ctacagcccg acctacgccc
      121 gctttgcctc tatcctgcgc ccttttatgt gcaacacttt gcacttgctg aacaaaattc
      181 aaacgaccct ttatatcaaa tgcaaaaaat atgccgtcat tcccgcgaa
//
LOCUS       DAXHRI010000349          227 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.349, whole genome shotgun sequence.
ACCESSION   DAXHRI010000349 DAXHRI010000000
VERSION     DAXHRI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.349"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           70 a           33 c           55 g           69 t
ORIGIN      
        1 tttaaaattt ttatattcac atagatgggg atgatgggat ttaggattct gattttgttt
       61 ttgagagaat gaaggaattt gagattgtgg gcgattatcg ggaaaaatag aatctttccg
      121 ccgtcattcc cgcgcaggcg ggaatctaga cattcaatgc taaggcaatt tatcgggaat
      181 gactgaaact caaaaaacta gattcccact ttcgtgggaa tgacgaa
//
LOCUS       DAXHRI010000350          225 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.350, whole genome shotgun sequence.
ACCESSION   DAXHRI010000350 DAXHRI010000000
VERSION     DAXHRI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.350"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           87 a           44 c           52 g           42 t
ORIGIN      
        1 ctgcggacgc gcattgtcgg gcagcaaaat tgtagcactg cgaaatgaga aagggagcta
       61 caacacgcca tcgaattcat agacgaactc ccacattgta gcactgcgaa atgagaaagg
      121 gagctacaac tgtatagaaa aaatcaaaat caaaaattta attgtagcac tgcgaaatga
      181 gaaagggagc tacaactata tgccgaagcc attactgaag tgaaa
//
LOCUS       DAXHRI010000351          222 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.351, whole genome shotgun sequence.
ACCESSION   DAXHRI010000351 DAXHRI010000000
VERSION     DAXHRI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.351"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           62 a           61 c           51 g           48 t
ORIGIN      
        1 aagccgtagg tcggatactt gtatccgaca aaagcctgcc atctcaaata gccgtcggat
       61 tcgagaatcc gacctgccaa accgggcgcg gacgctccgg ccggcagtta gtacgcaaat
      121 cgaacagaac atcacaaaaa agcccgattc ggattttcca atcgggcttt tttgcgcccg
      181 ttttgtcatc ccgtgaaata tccgcatgac aaaaatatag tg
//
LOCUS       DAXHRI010000352          221 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.352, whole genome shotgun sequence.
ACCESSION   DAXHRI010000352 DAXHRI010000000
VERSION     DAXHRI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.352"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           62 a           54 c           43 g           62 t
ORIGIN      
        1 tcatctgaat attttgccat aaaaaactgc accccctaaa gtcggtaagg tgtccaactt
       61 ttggggtgca gttcagaagc ggtctttttt tgcctgccgg ttttgaatca tcctccgtgt
      121 atattccctt gacgaaaaaa atgatgatat tacggatacc aaaactaagg tcgtatccgc
      181 ccccctactc tccctaagca aagagatgaa acagcgtatc g
//
LOCUS       DAXHRI010000353          215 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M03 241054
            SAMEA1706843-rid23335003.denovo.353, whole genome shotgun sequence.
ACCESSION   DAXHRI010000353 DAXHRI010000000
VERSION     DAXHRI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706843
            Sequence Read Archive: ERR484121
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378109.1
            Genome Coverage       :: 211x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 09:55:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,210
            CDSs (total)                      :: 2,150
            Genes (coding)                    :: 2,029
            CDSs (with protein)               :: 2,029
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 19 of 121
            Pseudo Genes (multiple problems)  :: 19 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706843-rid23335003.denovo.353"
                     /strain="M03 241054"
                     /db_xref="taxon:487"
BASE COUNT           75 a           30 c           39 g           71 t
ORIGIN      
        1 tatttccaaa gtttggtgaa tatgatttgg tgttaaatcc ggacattatt taattaagtt
       61 ttaaggccgt ctgaaaaaaa tttcaaacgg cttttattat tgggtttgga atctgaggat
      121 aaagctgata aaaaccagga aattatcaga ttgctatata cgtattgttg tacagactaa
      181 aggcagcaat caaatcacta ttgcttaccc acaaa
//
